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characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: library2 prokayotic 16S rRNA-ITS of core depth 128-129

text: aquatic

text: lake

text: sediment

text: Germany

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: sediment metagenome

text: sediment metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina MiSeq v3 2x300bp

characteristic: sequencing method


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: library2 prokayotic 16S rRNA-ITS of core depth 138-139

characteristic: description

text: aquatic

characteristic: environment (biome)

text: lake

characteristic: environment (feature)

text: sediment

text: Germany

text: mimarks-survey

text: sediment metagenome

text: sediment metagenome

text: sediment

text: Illumina MiSeq v3 2x300bp


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: library2 prokayotic 16S rRNA-ITS of core depth 148-149

text: aquatic

text: lake

text: sediment

characteristic: environment (material)

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: mimarks-survey

text: sediment metagenome

characteristic: organism

characteristic: project name

text: sediment metagenome

text: sediment

text: Illumina MiSeq v3 2x300bp

characteristic: title


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: library2 prokayotic 16S rRNA-ITS of core depth 158-159

text: aquatic

text: lake

text: sediment

text: Germany

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: sediment metagenome

text: sediment metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina MiSeq v3 2x300bp

characteristic: sequencing method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: library2 prokayotic 16S rRNA-ITS of core depth 168-169

characteristic: description

text: aquatic

characteristic: environment (biome)

text: lake

characteristic: environment (feature)

text: sediment

text: Germany

text: mimarks-survey

text: sediment metagenome

text: sediment metagenome

text: sediment

text: Illumina MiSeq v3 2x300bp


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: library2 prokayotic 16S rRNA-ITS of core depth 178-179

text: aquatic

text: lake

text: sediment

characteristic: environment (material)

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: mimarks-survey

text: sediment metagenome

characteristic: organism

characteristic: project name

text: sediment metagenome

text: sediment

text: Illumina MiSeq v3 2x300bp

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: library2 prokayotic 16S rRNA-ITS of core depth 188-189

text: aquatic

text: lake

text: sediment

text: Germany

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: sediment metagenome

text: sediment metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina MiSeq v3 2x300bp

characteristic: sequencing method


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: library2 prokayotic 16S rRNA-ITS of core depth 408-409

characteristic: description

text: aquatic

characteristic: environment (biome)

text: lake

characteristic: environment (feature)

text: sediment

text: Germany

text: mimarks-survey

text: sediment metagenome

text: sediment metagenome

text: sediment

text: Illumina MiSeq v3 2x300bp


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: library2 prokayotic 16S rRNA-ITS of NC_Ext

text: aquatic

text: lake

text: sediment

characteristic: environment (material)

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: mimarks-survey

text: sediment metagenome

characteristic: organism

characteristic: project name

text: sediment metagenome

text: sediment

text: Illumina MiSeq v3 2x300bp

text: Ext_Negative control_L2

characteristic: title


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: library2 prokayotic 16S rRNA-ITS of NC_PCR

text: aquatic

text: lake

text: sediment

text: Germany

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: sediment metagenome

text: Anthropogenic-induced increase in lake cyanobacteria abundance since the Bronze Age revealed by sedimentary ancient DNA reveals

text: sediment metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina MiSeq v3 2x300bp

characteristic: sequencing method

text: PCR_Negative control_L2


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: GFZ German Research Centre for Geosciences

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: library2 prokayotic 16S rRNA-ITS of PC

characteristic: description

text: aquatic

characteristic: environment (biome)

text: lake

characteristic: environment (feature)

text: sediment

text: Germany

text: mimarks-survey

text: sediment metagenome

text: sediment metagenome

text: sediment

text: Illumina MiSeq v3 2x300bp

text: Positive control_L2


text: INRAE Jouy-en-Josas

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

tag: Namespace:INRAE Jouy-en-Josas

text: rainbow trout

characteristic: common name

text: INRAE isogenic line 02 individual 2

text: Oncorhynchus mykiss

characteristic: organism

text: Oncorhynchus mykiss

text: female

characteristic: sex

text: fin

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: INRAE Jouy-en-Josas

text: public

text: rainbow trout

text: INRAE isogenic line 03 individual 2

text: Oncorhynchus mykiss

text: Oncorhynchus mykiss

text: female

text: fin


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRAE Jouy-en-Josas

text: public

text: rainbow trout

text: INRAE isogenic line 04

text: Oncorhynchus mykiss

text: Oncorhynchus mykiss

text: female

text: fin


text: INRAE Jouy-en-Josas

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: rainbow trout

text: INRAE isogenic line 12

text: Oncorhynchus mykiss

text: Oncorhynchus mykiss

text: female

text: fin


text: INRAE Jouy-en-Josas

text: public

text: rainbow trout

text: INRAE isogenic line 06 individual 2

characteristic: description

text: Oncorhynchus mykiss

text: Oncorhynchus mykiss

text: female

text: fin


text: INRAE Jouy-en-Josas

text: public

text: rainbow trout

text: INRAE isogenic line 13

text: Oncorhynchus mykiss

text: Oncorhynchus mykiss

text: female

text: fin


text: INRAE Jouy-en-Josas

text: public

text: rainbow trout

text: INRAE isogenic line 09 individual 2

text: Oncorhynchus mykiss

text: Oncorhynchus mykiss

text: female

text: fin


text: INRAE Jouy-en-Josas

text: public

text: rainbow trout

text: INRAE isogenic line 14

text: Oncorhynchus mykiss

text: Oncorhynchus mykiss

text: female

text: fin


text: INRAE Jouy-en-Josas

text: public

text: rainbow trout

text: INRAE isogenic line 15

text: Oncorhynchus mykiss

text: Oncorhynchus mykiss

text: female

text: fin


text: INRAE Jouy-en-Josas

text: public

text: rainbow trout

text: INRAE isogenic line 16

text: Oncorhynchus mykiss

text: Oncorhynchus mykiss

text: female

text: fin


text: INRAE Jouy-en-Josas

text: public

text: rainbow trout

text: INRAE isogenic line 17

text: Oncorhynchus mykiss

text: Oncorhynchus mykiss

text: female

text: fin


text: INRAE Jouy-en-Josas

text: public

text: rainbow trout

text: INRAE isogenic line 18

text: Oncorhynchus mykiss

text: Oncorhynchus mykiss

text: female

text: fin


text: INRAE Jouy-en-Josas

text: public

text: rainbow trout

text: INRAE isogenic line 19

text: Oncorhynchus mykiss

text: Oncorhynchus mykiss

text: female

text: fin


text: public

characteristic: collection date

text: mg_4-5 TSK19_H1_4-5_mg cm

text: aquatic

characteristic: environment (biome)

text: lake

text: sediment

characteristic: environment (material)

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: sediment metagenome

characteristic: project name

text: sediment metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina NovaSeq

characteristic: sequencing method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: mg_9-10 TSK19_H1_9-10_mg cm

text: aquatic

text: lake

characteristic: environment (feature)

text: sediment

text: Germany

text: metagenome

text: sediment metagenome

characteristic: organism

text: sediment metagenome

text: sediment

text: Illumina NovaSeq

characteristic: title


characteristic: External Id

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: mg_20-21 TSK19_H1_20-21_mg cm

characteristic: description

text: aquatic

characteristic: environment (biome)

text: lake

text: sediment

characteristic: environment (material)

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

text: metagenome

text: sediment metagenome

text: sediment metagenome

text: sediment

text: Illumina NovaSeq


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC center name

text: public

text: mg_210-211 TSK19_H2_10-11_mg cm

text: aquatic

text: lake

text: sediment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: sediment metagenome

characteristic: project name

text: sediment metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina NovaSeq

characteristic: sequencing method


characteristic: External Id

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: mg_260-261 TSK19_H2_60-61_mg cm

text: aquatic

text: lake

characteristic: environment (feature)

text: sediment

text: Germany

text: metagenome

text: sediment metagenome

characteristic: organism

text: sediment metagenome

text: sediment

text: Illumina NovaSeq

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC center name

text: public

text: mg_280-281 TSK19_H2_80-81_mg cm

characteristic: description

text: aquatic

characteristic: environment (biome)

text: lake

text: sediment

characteristic: environment (material)

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

text: metagenome

text: sediment metagenome

text: sediment metagenome

text: sediment

text: Illumina NovaSeq


characteristic: External Id

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: mg_385-386 TSK19_H2_185-186_mg cm

text: aquatic

text: lake

text: sediment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: sediment metagenome

characteristic: project name

text: sediment metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina NovaSeq

characteristic: sequencing method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: mg_525-526 TSK19_H3_125-126_mg cm

text: aquatic

text: lake

characteristic: environment (feature)

text: sediment

text: Germany

text: metagenome

text: sediment metagenome

characteristic: organism

text: sediment metagenome

text: sediment

text: Illumina NovaSeq

characteristic: title


characteristic: External Id

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: mg_650-651 TSK19_H4_50-51_mg cm

characteristic: description

text: aquatic

characteristic: environment (biome)

text: lake

text: sediment

characteristic: environment (material)

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

text: metagenome

text: sediment metagenome

text: sediment metagenome

text: sediment

text: Illumina NovaSeq


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC center name

text: public

text: mg_820-821 TSK19_H5_20-21_mg cm

text: aquatic

text: lake

text: sediment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: sediment metagenome

characteristic: project name

text: sediment metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina NovaSeq

characteristic: sequencing method


characteristic: External Id

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: mg_1015-1016 TSK19_H6_15-16_mg cm

text: aquatic

text: lake

characteristic: environment (feature)

text: sediment

text: Germany

text: metagenome

text: sediment metagenome

characteristic: organism

text: sediment metagenome

text: sediment

text: Illumina NovaSeq

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Marine Biological Association

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

characteristic: INSDC center name

text: public

text: NATURAL HISTORY MUSEUM

characteristic: barcoding center

text: COPO

characteristic: broker name

text: KES SCOTT-SOMME | RACHEL BRITTON

characteristic: collected by

text: MARINE BIOLOGICAL ASSOCIATION | MARINE BIOLOGICAL ASSOCIATION

characteristic: collecting institution

text: greater sand-eel

characteristic: common name

characteristic: depth

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Cornwall | whitsand bay

characteristic: geographic location (region and locality)

text: sand/mud

characteristic: habitat

text: KES SCOTT-SOMME

characteristic: identified by

text: MARINE BIOLOGICAL ASSOCIATION

text: adult

characteristic: lifestage

text: Hyperoplus immaculatus

text: WHOLE ORGANISM

characteristic: organism part

characteristic: original collection date

text: United Kingdom | Cornwall | whitsand bay

characteristic: original collection location

text: DTOL

text: Hyperoplus immaculatus

text: NOT COLLECTED

characteristic: sex

characteristic: specimen id

characteristic: specimen voucher

text: 4b3182d9-f6f4-4900-8032-19a5c222b618-DTOL-specimen

characteristic: tolid

type: derived from


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Marine Biological Association

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: NATURAL HISTORY MUSEUM

characteristic: barcoding center

text: COPO

characteristic: broker name

text: KES SCOTT-SOMME | RACHEL BRITTON

characteristic: collection date

text: greater sand-eel

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Cornwall | whitsand bay

text: sand/mud

text: KES SCOTT-SOMME

text: MARINE BIOLOGICAL ASSOCIATION

text: adult

text: Hyperoplus immaculatus

characteristic: organism

text: WHOLE ORGANISM

text: DTOL

characteristic: project name

text: Hyperoplus immaculatus

text: NOT COLLECTED

text: 4af23664-c8ca-493d-8a8b-203c79339ee9-DTOL-specimen

characteristic: title


Sga mRNA

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF OSLO

text: public

text: Sga mRNA

tag: Namespace:UNIVERSITY OF OSLO

text: mixed

text: mRNAs from Sphaeroforma gastrica

characteristic: description

text: mixed

text: Sphaeroforma gastrica

text: Sphaeroforma gastrica

text: Sga mRNA


E-MTAB-11618:Sample 3

characteristic: External Id

text: NEWCASTLE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11618:Sample 3

tag: Namespace:NEWCASTLE UNIVERSITY

text: ArrayExpress

text: human

characteristic: common name

text: Protocols: Samples were collected by the Human Developmental Biology Resource from the clinic and transported to the lab immediately before dissection. Livers were digested for30 mins using collagenase type 4 to create a single cell suspension. Nucleic acids were extracted following the manufacturers guidelines for the 10x Genomics Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry) (CG000204_ChromiumNextGEMSingleCell3'v3.1_Rev D.pdf).CITE-Seq libraries were amplified following the TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3' Reagent Kit v3 or v3.1 (Single Index) Protocol (https://www.biolegend.com/en-us/protocols/totalseq-a-antibodies-and-cell-hashing-with-10x-single-cell-3-reagent-kit-v3-3-1-protocol). Nucleic acid libraries were constructed following the manufacturers guidelines for the 10x Genomics Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry) (CG000204_ChromiumNextGEMSingleCell3'v3.1_Rev D.pdf).CITE-Seq libraries were constructed following the TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3' Reagent Kit v3 or v3.1 (Single Index) Protocol (https://www.biolegend.com/en-us/protocols/totalseq-a-antibodies-and-cell-hashing-with-10x-single-cell-3-reagent-kit-v3-3-1-protocol).

text: embryo stage

characteristic: developmental stage

text: normal

characteristic: disease

text: Carnegie stage 19, Carnegie stage 16, 11 weeks post conception

characteristic: embryo stage

characteristic: immunophenotype

text: F102, F110, F108

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: liver

characteristic: organism part

text: Homo sapiens

text: female

characteristic: sex

text: Sample 3


E-MTAB-11618:Sample 1

text: NEWCASTLE UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11618:Sample 1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: human

text: embryo stage

text: normal

text: F102, F110, F108

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: liver

text: Homo sapiens

text: female

text: Sample 1


E-MTAB-11618:Sample 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NEWCASTLE UNIVERSITY

text: public

text: E-MTAB-11618:Sample 2

text: ArrayExpress

text: human

characteristic: description

text: embryo stage

text: normal

text: Carnegie stage 19, 11 weeks post conception

text: F102, F108

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: female

text: Sample 2

characteristic: title


text: KAUST

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: collection date

text: bread wheat

characteristic: common name

text: introgression line Sr43 wild type

text: Israel

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Greenhouse

characteristic: isolation and growth condition

text: Triticum aestivum

text: seedlings

characteristic: plant developmental stage

text: soil mix

characteristic: plant growth medium

text: Roots

characteristic: plant structure

text: Triticum aestivum


characteristic: ENA-CHECKLIST

text: EBI

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR1621004 of study SRP002480.

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Skin

characteristic: environment (material)

text: USA

text: metagenome-assembled genome

characteristic: investigation type

text: human skin metagenome

text: human skin metagenome

characteristic: metagenomic source

text: Lawsonella clevelandensis

characteristic: organism

text: 16S rRNA gene sequences amplified from subjects with eczema and age-matched healthy controls. <p> Microbes living in and on humans are ten times more numerous than human cells. Culture-based methods have been the primary techniques used to study microbes inhabiting humans; however, many species are not successfully grown in culture. The NIH Roadmap for Medical Research Human Microbiome Project (HMP) aims to investigate the microbes in the gastrointestinal tract, oral cavity, skin, vagina, and nares. The goal of the HMP is to comprehensively characterize the human microbiota and analyze its role in human health and disease. <p> The skin serves not only as a barrier against invading pathogens and moisture loss, but also as a host to microbial communities. The skin of an adult is an approximately 2 square meter surface with a myriad of microenvironments. Atopic dermatitis (AD), more commonly known as eczema, is a common skin disorder that is exacerbated by Staphylococcus aureus colonization. This study aims to investigate the skin microbiota of AD patients at specific timepoints (quiescence, disease flares, and post-treatment) to examine how disease state correlates with changes in the skin microflora. <p> The 16S small subunit ribosomal (rRNA) gene is present in every bacterial cell, serving as a universal marker. The gene is sufficiently conserved to allow accurate alignment but adequately varied to enable phylogenetic analyses. Sequencing via 16S rRNA-based phylotyping has been used to survey the bacterial microbes on the skin. Initially, we will examine the bacterial diversity associated with AD using a 16S rRNA survey and later broadening to include fungi, viruses, archaea and mites. In addition, bacteria will be cultured on a wide variety of media and isolates representing both abundant and novel species will be selected for whole genome sequencing. <p> To characterize fungal diversity, we utilize two phylogenetic markers within the rDNA region: 18S rRNA and the Intervening Internal Transcribed Spacer (ITS) region. Full microbial diversity will be explored with shotgun metagenomic sequencing.

characteristic: project name

characteristic: sample derived from

text: Lawsonella clevelandensis

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR1621004_bin.14_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR1621010 of study SRP002480.

characteristic: description

text: Host-associated

text: Human

text: Skin

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: human skin metagenome

text: human skin metagenome

text: Staphylococcus auricularis

text: Staphylococcus auricularis

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: SRR1621010_bin.14_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR1623046 of study SRP002480.

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Skin

characteristic: environment (material)

text: USA

text: metagenome-assembled genome

characteristic: investigation type

text: human skin metagenome

text: human skin metagenome

characteristic: metagenomic source

text: uncultured Pseudomonas sp.

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: uncultured Pseudomonas sp.

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR1623046_bin.25_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR1630987 of study SRP002480.

characteristic: description

text: Host-associated

text: Human

text: Skin

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: human skin metagenome

text: human skin metagenome

text: Cutibacterium acnes

text: Cutibacterium acnes

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: SRR1630987_bin.21_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR1631065 of study SRP002480.

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Skin

characteristic: environment (material)

text: USA

text: metagenome-assembled genome

characteristic: investigation type

text: human skin metagenome

text: human skin metagenome

characteristic: metagenomic source

text: Streptococcus canis

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: Streptococcus canis

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR1631065_bin.46_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

text: CheckM

characteristic: description

text: Host-associated

text: Human

text: Skin

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: human skin metagenome

text: human skin metagenome

text: Gordonia bronchialis

text: Gordonia bronchialis

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: SRR1631065_bin.61_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Skin

characteristic: environment (material)

text: USA

text: metagenome-assembled genome

characteristic: investigation type

text: human skin metagenome

text: human skin metagenome

characteristic: metagenomic source

text: uncultured Simkaniaceae bacterium

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: uncultured Simkaniaceae bacterium

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR1631065_bin.62_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR1631109 of study SRP002480.

characteristic: description

text: Host-associated

text: Human

text: Skin

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: human skin metagenome

text: human skin metagenome

text: Gardnerella vaginalis

text: Gardnerella vaginalis

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: SRR1631109_bin.36_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR1631112 of study SRP002480.

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Skin

characteristic: environment (material)

text: USA

text: metagenome-assembled genome

characteristic: investigation type

text: human skin metagenome

text: human skin metagenome

characteristic: metagenomic source

text: Staphylococcus capitis

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: Staphylococcus capitis

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR1631112_bin.36_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR1631695 of study SRP002480.

characteristic: description

text: Host-associated

text: Human

text: Skin

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: human skin metagenome

text: human skin metagenome

text: uncultured Cutibacterium sp.

text: uncultured Cutibacterium sp.

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: SRR1631695_bin.22_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR1632388 of study SRP002480.

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Skin

characteristic: environment (material)

text: USA

text: metagenome-assembled genome

characteristic: investigation type

text: human skin metagenome

text: human skin metagenome

characteristic: metagenomic source

text: Corynebacterium hadale

characteristic: organism

text: 16S rRNA gene sequences amplified from subjects with eczema and age-matched healthy controls. <p> Microbes living in and on humans are ten times more numerous than human cells. Culture-based methods have been the primary techniques used to study microbes inhabiting humans; however, many species are not successfully grown in culture. The NIH Roadmap for Medical Research Human Microbiome Project (HMP) aims to investigate the microbes in the gastrointestinal tract, oral cavity, skin, vagina, and nares. The goal of the HMP is to comprehensively characterize the human microbiota and analyze its role in human health and disease. <p> The skin serves not only as a barrier against invading pathogens and moisture loss, but also as a host to microbial communities. The skin of an adult is an approximately 2 square meter surface with a myriad of microenvironments. Atopic dermatitis (AD), more commonly known as eczema, is a common skin disorder that is exacerbated by Staphylococcus aureus colonization. This study aims to investigate the skin microbiota of AD patients at specific timepoints (quiescence, disease flares, and post-treatment) to examine how disease state correlates with changes in the skin microflora. <p> The 16S small subunit ribosomal (rRNA) gene is present in every bacterial cell, serving as a universal marker. The gene is sufficiently conserved to allow accurate alignment but adequately varied to enable phylogenetic analyses. Sequencing via 16S rRNA-based phylotyping has been used to survey the bacterial microbes on the skin. Initially, we will examine the bacterial diversity associated with AD using a 16S rRNA survey and later broadening to include fungi, viruses, archaea and mites. In addition, bacteria will be cultured on a wide variety of media and isolates representing both abundant and novel species will be selected for whole genome sequencing. <p> To characterize fungal diversity, we utilize two phylogenetic markers within the rDNA region: 18S rRNA and the Intervening Internal Transcribed Spacer (ITS) region. Full microbial diversity will be explored with shotgun metagenomic sequencing.

characteristic: project name

characteristic: sample derived from

text: Corynebacterium hadale

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR1632388_bin.7_CONCOCT_v1.1_MAG