characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

text: uncultured Acholeplasmataceae bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Acholeplasmataceae bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901694_bin.60_CONCOCT_v1.1_MAG

characteristic: title


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR3901694 of study SRP056932.

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

text: uncultured actinobacterium

text: Gold mining and other industrial activities make extensive use of cyanide (CN-) and produce large volumes of thiocyanate- (SCN-) contaminated wastewater that must be treated before reuse in mining processes. Furthermore, contaminated wastewater stored in tailings dams may pose an environmental threat through the potential for leakage into local groundwater. Microbial communities are often used for biodegradation and environmental remediation, however, little is known about the membership and metabolic potential of SCN-degrading consortia. Application and improvement of the remediation strategy will benefit from an understanding of organisms that can contribute to the breakdown of SCN- into sulfur, carbon and nitrogen compounds. To this end, we performed metagenomic analysis of samples from two laboratory-scale bioreactors used to study SCN- and CN- degradation by established microbial consortia. Community analysis revealed the dominance of Thiobacillus spp. whose genomes harbor a previously unreported operon for SCN- degradation. Metabolic predictions suggest that a large portion of each bioreactor community is autotrophic, relying not on molasses in reactor feed, but using energy gained from oxidation of the sulfur compounds produced during SCN- degradation. Heterotrophs compose a smaller portion of the reactor bacterial community, and predation by phage and eukaryotes is predicted to affect community dynamics. Furthermore, genes for ammonium oxidation and denitrification were detected, possibly allowing removal of nitrogen as required for complete remediation of wastewater. These findings suggest possible optimization strategies for reactor design such as improved aerobic/anaerobic partitioning and elimination of organic carbon from reactor feed.

characteristic: project name

text: uncultured actinobacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901694_bin.69_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

text: uncultured Bacteroidia bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Bacteroidia bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901694_bin.72_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: project name

text: uncultured Clostridia bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901694_bin.76_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

text: uncultured Sphingobacteriales bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Sphingobacteriales bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901694_bin.79_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

text: uncultured Bacteroidetes bacterium

characteristic: project name

text: uncultured Bacteroidetes bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901694_bin.93_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR3901695 of study SRP056932.

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

text: Acidovorax radicis

characteristic: organism

characteristic: sample derived from

text: Acidovorax radicis

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901695_bin.102_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

text: uncultured Flavobacterium sp.

characteristic: project name

text: uncultured Flavobacterium sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901695_bin.114_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

text: uncultured Planctomycetales bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Planctomycetales bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901695_bin.14_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

text: uncultured Dyadobacter sp.

characteristic: project name

text: uncultured Dyadobacter sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901695_bin.18_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

text: uncultured Bacteroidetes bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Bacteroidetes bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901695_bin.34_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

text: uncultured Thiobacillus sp.

characteristic: project name

text: uncultured Thiobacillus sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901695_bin.40_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

text: uncultured Candidatus Saccharibacteria bacterium

characteristic: organism

characteristic: sample derived from

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901695_bin.43_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

text: uncultured Myxococcales bacterium

characteristic: project name

text: uncultured Myxococcales bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901695_bin.44_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

text: uncultured Sphingobacteriales bacterium

characteristic: organism

text: Gold mining and other industrial activities make extensive use of cyanide (CN-) and produce large volumes of thiocyanate- (SCN-) contaminated wastewater that must be treated before reuse in mining processes. Furthermore, contaminated wastewater stored in tailings dams may pose an environmental threat through the potential for leakage into local groundwater. Microbial communities are often used for biodegradation and environmental remediation, however, little is known about the membership and metabolic potential of SCN-degrading consortia. Application and improvement of the remediation strategy will benefit from an understanding of organisms that can contribute to the breakdown of SCN- into sulfur, carbon and nitrogen compounds. To this end, we performed metagenomic analysis of samples from two laboratory-scale bioreactors used to study SCN- and CN- degradation by established microbial consortia. Community analysis revealed the dominance of Thiobacillus spp. whose genomes harbor a previously unreported operon for SCN- degradation. Metabolic predictions suggest that a large portion of each bioreactor community is autotrophic, relying not on molasses in reactor feed, but using energy gained from oxidation of the sulfur compounds produced during SCN- degradation. Heterotrophs compose a smaller portion of the reactor bacterial community, and predation by phage and eukaryotes is predicted to affect community dynamics. Furthermore, genes for ammonium oxidation and denitrification were detected, possibly allowing removal of nitrogen as required for complete remediation of wastewater. These findings suggest possible optimization strategies for reactor design such as improved aerobic/anaerobic partitioning and elimination of organic carbon from reactor feed.

characteristic: sample derived from

text: uncultured Sphingobacteriales bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901695_bin.72_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

text: uncultured Magnetospirillum sp.

characteristic: project name

text: uncultured Magnetospirillum sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901695_bin.8_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

text: uncultured Sphingobacteriales bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Sphingobacteriales bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901695_bin.83_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

text: uncultured Gemmobacter sp.

characteristic: project name

text: uncultured Gemmobacter sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901695_bin.9_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR3901696 of study SRP056932.

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

text: uncultured Sphingobacteriales bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Sphingobacteriales bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901696_bin.16_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

text: uncultured Novosphingobium sp.

characteristic: project name

text: uncultured Novosphingobium sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901696_bin.21_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

text: uncultured Leifsonia sp.

characteristic: organism

characteristic: sample derived from

text: uncultured Leifsonia sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901696_bin.78_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR3901697 of study SRP056932.

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

text: uncultured Bosea sp.

characteristic: project name

text: uncultured Bosea sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901697_bin.14_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

text: uncultured Nitrosospira sp.

characteristic: organism

characteristic: sample derived from

text: uncultured Nitrosospira sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901697_bin.19_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

text: uncultured Terrimonas sp.

characteristic: project name

text: uncultured Terrimonas sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901697_bin.2_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

text: uncultured Acidovorax sp.

characteristic: organism

characteristic: sample derived from

text: uncultured Acidovorax sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901697_bin.34_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

text: uncultured Burkholderiales bacterium

characteristic: project name

text: uncultured Burkholderiales bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901697_bin.39_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR3901698 of study SRP056932.

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

text: uncultured bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901698_bin.10_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

text: uncultured Novosphingobium sp.

text: Gold mining and other industrial activities make extensive use of cyanide (CN-) and produce large volumes of thiocyanate- (SCN-) contaminated wastewater that must be treated before reuse in mining processes. Furthermore, contaminated wastewater stored in tailings dams may pose an environmental threat through the potential for leakage into local groundwater. Microbial communities are often used for biodegradation and environmental remediation, however, little is known about the membership and metabolic potential of SCN-degrading consortia. Application and improvement of the remediation strategy will benefit from an understanding of organisms that can contribute to the breakdown of SCN- into sulfur, carbon and nitrogen compounds. To this end, we performed metagenomic analysis of samples from two laboratory-scale bioreactors used to study SCN- and CN- degradation by established microbial consortia. Community analysis revealed the dominance of Thiobacillus spp. whose genomes harbor a previously unreported operon for SCN- degradation. Metabolic predictions suggest that a large portion of each bioreactor community is autotrophic, relying not on molasses in reactor feed, but using energy gained from oxidation of the sulfur compounds produced during SCN- degradation. Heterotrophs compose a smaller portion of the reactor bacterial community, and predation by phage and eukaryotes is predicted to affect community dynamics. Furthermore, genes for ammonium oxidation and denitrification were detected, possibly allowing removal of nitrogen as required for complete remediation of wastewater. These findings suggest possible optimization strategies for reactor design such as improved aerobic/anaerobic partitioning and elimination of organic carbon from reactor feed.

characteristic: project name

text: uncultured Novosphingobium sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901698_bin.109_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

text: uncultured Sulfurimonas sp.

characteristic: organism

characteristic: sample derived from

text: uncultured Sulfurimonas sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901698_bin.24_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

text: uncultured Sphingobium sp.

characteristic: project name

text: uncultured Sphingobium sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901698_bin.25_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

text: Candidatus Altiarchaeota archaeon

characteristic: organism

characteristic: sample derived from

text: Candidatus Altiarchaeota archaeon

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901698_bin.30_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

text: uncultured Sphingobacteriales bacterium

characteristic: project name

text: uncultured Sphingobacteriales bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901698_bin.4_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

text: uncultured Thiobacillus sp.

characteristic: organism

characteristic: sample derived from

text: uncultured Thiobacillus sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901698_bin.54_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

text: uncultured Alphaproteobacteria bacterium

characteristic: project name

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901698_bin.6_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

text: uncultured Microbacterium sp.

characteristic: organism

characteristic: sample derived from

text: uncultured Microbacterium sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901698_bin.67_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

text: uncultured Bacteroidetes bacterium

characteristic: project name

text: uncultured Bacteroidetes bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901698_bin.7_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

text: Salana multivorans

characteristic: organism

characteristic: sample derived from

text: Salana multivorans

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901698_bin.75_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

text: uncultured Devosia sp.

characteristic: project name

text: uncultured Devosia sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901698_bin.80_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

text: uncultured Burkholderiales bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Burkholderiales bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901698_bin.98_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR3901699 of study SRP056932.

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

text: uncultured Chlamydiales bacterium

characteristic: project name

text: uncultured Chlamydiales bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901699_bin.0_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

text: uncultured Variovorax sp.

characteristic: organism

text: Gold mining and other industrial activities make extensive use of cyanide (CN-) and produce large volumes of thiocyanate- (SCN-) contaminated wastewater that must be treated before reuse in mining processes. Furthermore, contaminated wastewater stored in tailings dams may pose an environmental threat through the potential for leakage into local groundwater. Microbial communities are often used for biodegradation and environmental remediation, however, little is known about the membership and metabolic potential of SCN-degrading consortia. Application and improvement of the remediation strategy will benefit from an understanding of organisms that can contribute to the breakdown of SCN- into sulfur, carbon and nitrogen compounds. To this end, we performed metagenomic analysis of samples from two laboratory-scale bioreactors used to study SCN- and CN- degradation by established microbial consortia. Community analysis revealed the dominance of Thiobacillus spp. whose genomes harbor a previously unreported operon for SCN- degradation. Metabolic predictions suggest that a large portion of each bioreactor community is autotrophic, relying not on molasses in reactor feed, but using energy gained from oxidation of the sulfur compounds produced during SCN- degradation. Heterotrophs compose a smaller portion of the reactor bacterial community, and predation by phage and eukaryotes is predicted to affect community dynamics. Furthermore, genes for ammonium oxidation and denitrification were detected, possibly allowing removal of nitrogen as required for complete remediation of wastewater. These findings suggest possible optimization strategies for reactor design such as improved aerobic/anaerobic partitioning and elimination of organic carbon from reactor feed.

characteristic: sample derived from

text: uncultured Variovorax sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901699_bin.104_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

text: uncultured Chlamydia sp.

characteristic: project name

text: uncultured Chlamydia sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901699_bin.47_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

text: uncultured Candidatus Saccharibacteria bacterium

characteristic: organism

characteristic: sample derived from

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901699_bin.76_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR3901700 of study SRP056932.

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

text: uncultured Bacteroidales bacterium

characteristic: project name

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901700_bin.119_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

text: uncultured Sphingobacteriales bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Sphingobacteriales bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901700_bin.15_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

text: uncultured Sphingobacterium sp.

characteristic: project name

text: uncultured Sphingobacterium sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901700_bin.18_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

text: uncultured Microbacterium sp.

characteristic: organism

characteristic: sample derived from

text: uncultured Microbacterium sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901700_bin.23_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

text: uncultured Micrococcales bacterium

characteristic: project name

text: uncultured Micrococcales bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901700_bin.25_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

text: uncultured Alphaproteobacteria bacterium

characteristic: organism

characteristic: sample derived from

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901700_bin.36_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

characteristic: project name

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901700_bin.4_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

text: uncultured Dysgonomonas sp.

characteristic: organism

characteristic: sample derived from

text: uncultured Dysgonomonas sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901700_bin.52_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

text: uncultured Flavobacteriia bacterium

characteristic: project name

text: uncultured Flavobacteriia bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901700_bin.58_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

text: uncultured Legionella sp.

characteristic: organism

text: Gold mining and other industrial activities make extensive use of cyanide (CN-) and produce large volumes of thiocyanate- (SCN-) contaminated wastewater that must be treated before reuse in mining processes. Furthermore, contaminated wastewater stored in tailings dams may pose an environmental threat through the potential for leakage into local groundwater. Microbial communities are often used for biodegradation and environmental remediation, however, little is known about the membership and metabolic potential of SCN-degrading consortia. Application and improvement of the remediation strategy will benefit from an understanding of organisms that can contribute to the breakdown of SCN- into sulfur, carbon and nitrogen compounds. To this end, we performed metagenomic analysis of samples from two laboratory-scale bioreactors used to study SCN- and CN- degradation by established microbial consortia. Community analysis revealed the dominance of Thiobacillus spp. whose genomes harbor a previously unreported operon for SCN- degradation. Metabolic predictions suggest that a large portion of each bioreactor community is autotrophic, relying not on molasses in reactor feed, but using energy gained from oxidation of the sulfur compounds produced during SCN- degradation. Heterotrophs compose a smaller portion of the reactor bacterial community, and predation by phage and eukaryotes is predicted to affect community dynamics. Furthermore, genes for ammonium oxidation and denitrification were detected, possibly allowing removal of nitrogen as required for complete remediation of wastewater. These findings suggest possible optimization strategies for reactor design such as improved aerobic/anaerobic partitioning and elimination of organic carbon from reactor feed.

characteristic: sample derived from

text: uncultured Legionella sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901700_bin.93_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Multiple fragments where gaps span repetitive regions. Presence of the 23S, 16S, and 5S rRNA genes and at least 18 tRNAs

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR3901701 of study SRP056932.

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

text: uncultured Armatimonadetes bacterium

characteristic: project name

text: uncultured Armatimonadetes bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901701_bin.101_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

characteristic: organism

characteristic: sample derived from

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901701_bin.105_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

text: uncultured Sphingobium sp.

characteristic: project name

text: uncultured Sphingobium sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901701_bin.2_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

text: uncultured Rhizobium sp.

characteristic: organism

characteristic: sample derived from

text: uncultured Rhizobium sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901701_bin.3_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

characteristic: project name

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901701_bin.31_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

characteristic: organism

characteristic: sample derived from

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901701_bin.32_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

text: uncultured Micrococcales bacterium

characteristic: project name

text: uncultured Micrococcales bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901701_bin.41_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

text: uncultured Rhizobium sp.

characteristic: organism

characteristic: sample derived from

text: uncultured Rhizobium sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901701_bin.43_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

text: uncultured Bacteroidetes bacterium

characteristic: project name

text: uncultured Bacteroidetes bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901701_bin.57_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

text: uncultured Devosia sp.

characteristic: organism

characteristic: sample derived from

text: uncultured Devosia sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901701_bin.62_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

text: uncultured Chryseobacterium sp.

characteristic: project name

text: uncultured Chryseobacterium sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901701_bin.77_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

text: uncultured Spirosoma sp.

characteristic: organism

characteristic: sample derived from

text: uncultured Spirosoma sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901701_bin.89_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

text: uncultured Mesorhizobium sp.

text: Gold mining and other industrial activities make extensive use of cyanide (CN-) and produce large volumes of thiocyanate- (SCN-) contaminated wastewater that must be treated before reuse in mining processes. Furthermore, contaminated wastewater stored in tailings dams may pose an environmental threat through the potential for leakage into local groundwater. Microbial communities are often used for biodegradation and environmental remediation, however, little is known about the membership and metabolic potential of SCN-degrading consortia. Application and improvement of the remediation strategy will benefit from an understanding of organisms that can contribute to the breakdown of SCN- into sulfur, carbon and nitrogen compounds. To this end, we performed metagenomic analysis of samples from two laboratory-scale bioreactors used to study SCN- and CN- degradation by established microbial consortia. Community analysis revealed the dominance of Thiobacillus spp. whose genomes harbor a previously unreported operon for SCN- degradation. Metabolic predictions suggest that a large portion of each bioreactor community is autotrophic, relying not on molasses in reactor feed, but using energy gained from oxidation of the sulfur compounds produced during SCN- degradation. Heterotrophs compose a smaller portion of the reactor bacterial community, and predation by phage and eukaryotes is predicted to affect community dynamics. Furthermore, genes for ammonium oxidation and denitrification were detected, possibly allowing removal of nitrogen as required for complete remediation of wastewater. These findings suggest possible optimization strategies for reactor design such as improved aerobic/anaerobic partitioning and elimination of organic carbon from reactor feed.

characteristic: project name

text: uncultured Mesorhizobium sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901701_bin.95_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR3901702 of study SRP056932.

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

text: uncultured Alphaproteobacteria bacterium

characteristic: organism

characteristic: sample derived from

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901702_bin.0_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

text: uncultured Chryseobacterium sp.

characteristic: project name

text: uncultured Chryseobacterium sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901702_bin.10_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

text: uncultured Acidobacteriales bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Acidobacteriales bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901702_bin.25_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

text: uncultured Sphingomonas sp.

characteristic: project name

text: uncultured Sphingomonas sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901702_bin.29_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

text: Hyphomicrobium denitrificans

characteristic: organism

characteristic: sample derived from

text: Hyphomicrobium denitrificans

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901702_bin.60_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

text: uncultured Sphingomonas sp.

characteristic: project name

text: uncultured Sphingomonas sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901702_bin.63_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

text: uncultured Microbacterium sp.

characteristic: organism

characteristic: sample derived from

text: uncultured Microbacterium sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901702_bin.71_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

characteristic: project name

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901702_bin.9_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR3901703 of study SRP056932.

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

text: uncultured Rhodospirillales bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Rhodospirillales bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901703_bin.59_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

characteristic: project name

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901703_bin.65_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR3901704 of study SRP056932.

characteristic: description

text: Engineered

text: Engineered

text: Engineered

text: South Africa

text: metagenome-assembled genome

text: metagenome

text: metagenome

text: uncultured actinobacterium

characteristic: organism

characteristic: sample derived from

text: uncultured actinobacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3901704_bin.37_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Engineered

characteristic: environment (feature)

text: Engineered

characteristic: environment (material)

text: South Africa

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: metagenome

text: metagenome

characteristic: metagenomic source

text: Gold mining and other industrial activities make extensive use of cyanide (CN-) and produce large volumes of thiocyanate- (SCN-) contaminated wastewater that must be treated before reuse in mining processes. Furthermore, contaminated wastewater stored in tailings dams may pose an environmental threat through the potential for leakage into local groundwater. Microbial communities are often used for biodegradation and environmental remediation, however, little is known about the membership and metabolic potential of SCN-degrading consortia. Application and improvement of the remediation strategy will benefit from an understanding of organisms that can contribute to the breakdown of SCN- into sulfur, carbon and nitrogen compounds. To this end, we performed metagenomic analysis of samples from two laboratory-scale bioreactors used to study SCN- and CN- degradation by established microbial consortia. Community analysis revealed the dominance of Thiobacillus spp. whose genomes harbor a previously unreported operon for SCN- degradation. Metabolic predictions suggest that a large portion of each bioreactor community is autotrophic, relying not on molasses in reactor feed, but using energy gained from oxidation of the sulfur compounds produced during SCN- degradation. Heterotrophs compose a smaller portion of the reactor bacterial community, and predation by phage and eukaryotes is predicted to affect community dynamics. Furthermore, genes for ammonium oxidation and denitrification were detected, possibly allowing removal of nitrogen as required for complete remediation of wastewater. These findings suggest possible optimization strategies for reactor design such as improved aerobic/anaerobic partitioning and elimination of organic carbon from reactor feed.

characteristic: project name

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3901704_bin.42_CONCOCT_v1.1_MAG