characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.14.1

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Environmental

text: Aquatic

text: Freshwater

text: India

text: metagenome-assembled genome

text: freshwater metagenome

text: freshwater metagenome

text: uncultured bacterium

characteristic: organism

text: uncultured bacterium

text: NextSeq 500

text: multi-marker approach

text: Metagenome-assembled genome: SRR8868559_bin.180_CONCOCT_v1.1_MAG

characteristic: title


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes v3.14.1

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR8868559 of study SRP191499.

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Freshwater

characteristic: environment (material)

text: India

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: freshwater metagenome

text: freshwater metagenome

characteristic: metagenomic source

text: uncultured Paludibacter sp.

text: To understand the microbial communities found in the contaminated sediments of the river Yamuna.

characteristic: project name

characteristic: sample derived from

text: uncultured Paludibacter sp.

text: NextSeq 500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR8868559_bin.182_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.14.1

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Environmental

text: Aquatic

text: Freshwater

text: India

text: metagenome-assembled genome

text: freshwater metagenome

text: freshwater metagenome

text: uncultured Alphaproteobacteria bacterium

characteristic: organism

text: NextSeq 500

text: multi-marker approach

text: Metagenome-assembled genome: SRR8868559_bin.26_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.14.1

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Freshwater

characteristic: environment (material)

text: India

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: freshwater metagenome

text: freshwater metagenome

characteristic: metagenomic source

text: uncultured Thermomonas sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Thermomonas sp.

text: NextSeq 500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR8868559_bin.38_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.14.1

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Environmental

text: Aquatic

text: Freshwater

text: India

text: metagenome-assembled genome

text: freshwater metagenome

text: freshwater metagenome

text: uncultured Sphaerochaeta sp.

characteristic: organism

text: uncultured Sphaerochaeta sp.

text: NextSeq 500

text: multi-marker approach

text: Metagenome-assembled genome: SRR8868559_bin.41_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.14.1

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Freshwater

characteristic: environment (material)

text: India

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: freshwater metagenome

text: freshwater metagenome

characteristic: metagenomic source

text: uncultured Desulfobacteraceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Desulfobacteraceae bacterium

text: NextSeq 500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR8868559_bin.44_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.14.1

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Environmental

text: Aquatic

text: Freshwater

text: India

text: metagenome-assembled genome

text: freshwater metagenome

text: freshwater metagenome

text: uncultured Bacteroidetes bacterium

characteristic: organism

text: uncultured Bacteroidetes bacterium

text: NextSeq 500

text: multi-marker approach

text: Metagenome-assembled genome: SRR8868559_bin.49_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.14.1

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Freshwater

characteristic: environment (material)

text: India

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: freshwater metagenome

text: freshwater metagenome

characteristic: metagenomic source

text: uncultured proteobacterium

characteristic: project name

characteristic: sample derived from

text: uncultured proteobacterium

text: NextSeq 500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR8868559_bin.50_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.14.1

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Environmental

text: Aquatic

text: Freshwater

text: India

text: metagenome-assembled genome

text: freshwater metagenome

text: freshwater metagenome

text: uncultured Desulfomicrobium sp.

characteristic: organism

text: uncultured Desulfomicrobium sp.

text: NextSeq 500

text: multi-marker approach

text: Metagenome-assembled genome: SRR8868559_bin.55_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.14.1

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Freshwater

characteristic: environment (material)

text: India

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: freshwater metagenome

text: freshwater metagenome

characteristic: metagenomic source

text: uncultured Anaerolineaceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Anaerolineaceae bacterium

text: NextSeq 500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR8868559_bin.57_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.14.1

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Environmental

text: Aquatic

text: Freshwater

text: India

text: metagenome-assembled genome

text: freshwater metagenome

text: freshwater metagenome

text: uncultured Holophagaceae bacterium

characteristic: organism

text: uncultured Holophagaceae bacterium

text: NextSeq 500

text: multi-marker approach

text: Metagenome-assembled genome: SRR8868559_bin.79_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.14.1

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Freshwater

characteristic: environment (material)

text: India

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: freshwater metagenome

text: freshwater metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: NextSeq 500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR8868559_bin.82_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.14.1

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Environmental

text: Aquatic

text: Freshwater

text: India

text: metagenome-assembled genome

text: freshwater metagenome

text: freshwater metagenome

text: uncultured bacterium

characteristic: organism

text: uncultured bacterium

text: NextSeq 500

text: multi-marker approach

text: Metagenome-assembled genome: SRR8868559_bin.84_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes version 3.10.139

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1414211 of study ERP015657.

text: Engineered

characteristic: environment (biome)

text: Wastewater

characteristic: environment (feature)

text: Wastewater

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: wastewater metagenome

text: wastewater metagenome

characteristic: metagenomic source

text: uncultured Ruminococcus sp.

text: Sewage treatment plants (STPs) have repeatedly been suggested as “hotspots” for the emergence and dissemination of antibiotic-resistant pathogens. A critical question still unanswered is if selection pressures within STPs, caused by residual antibiotics or other co-selective agents, are sufficient to specifically promote resistance. To address this, we employed shotgun metagenomic sequencing of samples from different steps of the treatment process in three Swedish STPs. In parallel, concentrations of selected antibiotics, biocides and metals were analyzed.

characteristic: project name

characteristic: sample derived from

text: uncultured Ruminococcus sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1414211_bin.38_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1414267 of study ERP015657.

characteristic: description

text: Engineered

text: Wastewater

text: Wastewater

text: Sweden

text: metagenome-assembled genome

text: wastewater metagenome

text: wastewater metagenome

text: Lactococcus raffinolactis

characteristic: organism

text: Lactococcus raffinolactis

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1414267_bin.11_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes version 3.10.139

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Wastewater

characteristic: environment (feature)

text: Wastewater

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: wastewater metagenome

text: wastewater metagenome

characteristic: metagenomic source

text: Streptococcus suis

characteristic: project name

characteristic: sample derived from

text: Streptococcus suis

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1414267_bin.21_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Engineered

text: Wastewater

text: Wastewater

text: Sweden

text: metagenome-assembled genome

text: wastewater metagenome

text: wastewater metagenome

text: uncultured Prevotella sp.

characteristic: organism

text: uncultured Prevotella sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1414267_bin.24_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes version 3.10.139

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Wastewater

characteristic: environment (feature)

text: Wastewater

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: wastewater metagenome

text: wastewater metagenome

characteristic: metagenomic source

text: uncultured Acidimicrobiales bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Acidimicrobiales bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1414267_bin.40_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Multiple fragments where gaps span repetitive regions. Presence of the 23S, 16S, and 5S rRNA genes and at least 18 tRNAs

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Engineered

text: Wastewater

text: Wastewater

text: Sweden

text: metagenome-assembled genome

text: wastewater metagenome

text: wastewater metagenome

text: uncultured Coriobacteriales bacterium

characteristic: organism

text: uncultured Coriobacteriales bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1414267_bin.44_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes version 3.10.139

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Wastewater

characteristic: environment (feature)

text: Wastewater

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: wastewater metagenome

text: wastewater metagenome

characteristic: metagenomic source

text: uncultured Saprospiraceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Saprospiraceae bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1414267_bin.48_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Engineered

text: Wastewater

text: Wastewater

text: Sweden

text: metagenome-assembled genome

text: wastewater metagenome

text: wastewater metagenome

text: uncultured Acidimicrobiaceae bacterium

characteristic: organism

text: uncultured Acidimicrobiaceae bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1414267_bin.52_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes version 3.10.139

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Wastewater

characteristic: environment (feature)

text: Wastewater

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: wastewater metagenome

text: wastewater metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1414267_bin.67_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Engineered

text: Wastewater

text: Wastewater

text: Sweden

text: metagenome-assembled genome

text: wastewater metagenome

text: wastewater metagenome

text: uncultured Prevotella sp.

characteristic: organism

text: uncultured Prevotella sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1414267_bin.9_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes version 3.10.139

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1414271 of study ERP015657.

text: Engineered

characteristic: environment (biome)

text: Wastewater

characteristic: environment (feature)

text: Wastewater

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: wastewater metagenome

text: wastewater metagenome

characteristic: metagenomic source

text: uncultured Bacteroidales bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1414271_bin.44_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1414273 of study ERP015657.

characteristic: description

text: Engineered

text: Wastewater

text: Wastewater

text: Sweden

text: metagenome-assembled genome

text: wastewater metagenome

text: wastewater metagenome

text: uncultured Ruminococcus sp.

characteristic: organism

text: uncultured Ruminococcus sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1414273_bin.0_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes version 3.10.139

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Wastewater

characteristic: environment (feature)

text: Wastewater

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: wastewater metagenome

text: wastewater metagenome

characteristic: metagenomic source

text: uncultured Fusobacteriaceae bacterium

text: Sewage treatment plants (STPs) have repeatedly been suggested as “hotspots” for the emergence and dissemination of antibiotic-resistant pathogens. A critical question still unanswered is if selection pressures within STPs, caused by residual antibiotics or other co-selective agents, are sufficient to specifically promote resistance. To address this, we employed shotgun metagenomic sequencing of samples from different steps of the treatment process in three Swedish STPs. In parallel, concentrations of selected antibiotics, biocides and metals were analyzed.

characteristic: project name

characteristic: sample derived from

text: uncultured Fusobacteriaceae bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1414273_bin.105_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Engineered

text: Wastewater

text: Wastewater

text: Sweden

text: metagenome-assembled genome

text: wastewater metagenome

text: wastewater metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text: uncultured Lachnospiraceae bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1414273_bin.30_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes version 3.10.139

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Wastewater

characteristic: environment (feature)

text: Wastewater

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: wastewater metagenome

text: wastewater metagenome

characteristic: metagenomic source

text: uncultured Bacteroidales bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1414273_bin.64_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Engineered

text: Wastewater

text: Wastewater

text: Sweden

text: metagenome-assembled genome

text: wastewater metagenome

text: wastewater metagenome

text: uncultured Moraxella sp.

characteristic: organism

text: uncultured Moraxella sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1414273_bin.70_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes version 3.10.139

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Wastewater

characteristic: environment (feature)

text: Wastewater

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: wastewater metagenome

text: wastewater metagenome

characteristic: metagenomic source

text: uncultured Lachnospiraceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1414273_bin.76_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR2113683 of study ERP024437.

characteristic: description

text: Engineered

text: Wastewater

text: Wastewater

text: Denmark

text: metagenome-assembled genome

text: wastewater metagenome

text: wastewater metagenome

text: uncultured bacterium

characteristic: organism

text: Members of the candidate phylum Acetothermia are globally distributed and have been detected in diverse natural habitats as well as engineered systems. However, little is known about their physiology and ecological importance. In this study, an OTU belonging to Acetothermia was identified as one of the most abundant genus-level bacterial taxa in full-scale mesophilic anaerobic digesters at two Danish wastewater treatment plants. A closed genome was obtained by differential coverage binning of metagenomes and long Nanopore reads. Genome annotation and metabolic reconstruction revealed an anaerobic chemoheterotrophic lifestyle in which the bacterium obtain energy and carbon from the fermentation of peptides, amino acids, and simple sugars to acetate, formate and hydrogen. In contrast to previously studied Acetothermia, the genome did not encode a functional acetyl-CoA pathway required for acetogenesis. Fluorescent in situ hybridization probes targeting Acetothermia were designed and used in combination with confocal laser scanning microscopy and atomic force microscopy. This revealed an unusual morphology composed of a central rod cell with bipolar prosthecae similar to those observed in Caulobacter and Asticcacaulis. These prosthecae greatly expand the cell surface to volume ratio and allow for increased nutrient uptake, providing the bacteria with a competitive advantage under nutrient limited conditions.

text: uncultured bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR2113683_bin.0_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Wastewater

characteristic: environment (feature)

text: Wastewater

characteristic: environment (material)

text: Denmark

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: wastewater metagenome

text: wastewater metagenome

characteristic: metagenomic source

text: uncultured Anaerolineaceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Anaerolineaceae bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR2113683_bin.125_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Multiple fragments where gaps span repetitive regions. Presence of the 23S, 16S, and 5S rRNA genes and at least 18 tRNAs

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Engineered

text: Wastewater

text: Wastewater

text: Denmark

text: metagenome-assembled genome

text: wastewater metagenome

text: wastewater metagenome

text: uncultured Spirochaetaceae bacterium

characteristic: organism

text: uncultured Spirochaetaceae bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR2113683_bin.27_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Wastewater

characteristic: environment (feature)

text: Wastewater

characteristic: environment (material)

text: Denmark

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: wastewater metagenome

text: wastewater metagenome

characteristic: metagenomic source

text: uncultured Syntrophaceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Syntrophaceae bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR2113683_bin.45_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Engineered

text: Wastewater

text: Wastewater

text: Denmark

text: metagenome-assembled genome

text: wastewater metagenome

text: wastewater metagenome

text: uncultured Anaerolineaceae bacterium

characteristic: organism

text: uncultured Anaerolineaceae bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR2113683_bin.54_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR2113684 of study ERP024437.

text: Engineered

characteristic: environment (biome)

text: Wastewater

characteristic: environment (feature)

text: Wastewater

characteristic: environment (material)

text: Denmark

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: wastewater metagenome

text: wastewater metagenome

characteristic: metagenomic source

text: uncultured Syntrophaceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Syntrophaceae bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR2113684_bin.127_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Engineered

text: Wastewater

text: Wastewater

text: Denmark

text: metagenome-assembled genome

text: wastewater metagenome

text: wastewater metagenome

text: uncultured bacterium

characteristic: organism

text: uncultured bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR2113684_bin.59_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Wastewater

characteristic: environment (feature)

text: Wastewater

characteristic: environment (material)

text: Denmark

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: wastewater metagenome

text: wastewater metagenome

characteristic: metagenomic source

text: uncultured Firmicutes bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Firmicutes bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR2113684_bin.63_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR2113685 of study ERP024437.

characteristic: description

text: Engineered

text: Wastewater

text: Wastewater

text: Denmark

text: metagenome-assembled genome

text: wastewater metagenome

text: wastewater metagenome

text: uncultured Prolixibacteraceae bacterium

characteristic: organism

text: uncultured Prolixibacteraceae bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR2113685_bin.105_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Wastewater

characteristic: environment (feature)

text: Wastewater

characteristic: environment (material)

text: Denmark

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: wastewater metagenome

text: wastewater metagenome

characteristic: metagenomic source

text: uncultured Synergistales bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Synergistales bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR2113685_bin.131_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Engineered

text: Wastewater

text: Wastewater

text: Denmark

text: metagenome-assembled genome

text: wastewater metagenome

text: wastewater metagenome

text: uncultured Clostridia bacterium

characteristic: organism

text: uncultured Clostridia bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR2113685_bin.133_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR2113686 of study ERP024437.

text: Engineered

characteristic: environment (biome)

text: Wastewater

characteristic: environment (feature)

text: Wastewater

characteristic: environment (material)

text: Denmark

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: wastewater metagenome

text: wastewater metagenome

characteristic: metagenomic source

text: uncultured Syntrophaceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Syntrophaceae bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR2113686_bin.126_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Engineered

text: Wastewater

text: Wastewater

text: Denmark

text: metagenome-assembled genome

text: wastewater metagenome

text: wastewater metagenome

text: uncultured Anaerolineaceae bacterium

characteristic: organism

text: Members of the candidate phylum Acetothermia are globally distributed and have been detected in diverse natural habitats as well as engineered systems. However, little is known about their physiology and ecological importance. In this study, an OTU belonging to Acetothermia was identified as one of the most abundant genus-level bacterial taxa in full-scale mesophilic anaerobic digesters at two Danish wastewater treatment plants. A closed genome was obtained by differential coverage binning of metagenomes and long Nanopore reads. Genome annotation and metabolic reconstruction revealed an anaerobic chemoheterotrophic lifestyle in which the bacterium obtain energy and carbon from the fermentation of peptides, amino acids, and simple sugars to acetate, formate and hydrogen. In contrast to previously studied Acetothermia, the genome did not encode a functional acetyl-CoA pathway required for acetogenesis. Fluorescent in situ hybridization probes targeting Acetothermia were designed and used in combination with confocal laser scanning microscopy and atomic force microscopy. This revealed an unusual morphology composed of a central rod cell with bipolar prosthecae similar to those observed in Caulobacter and Asticcacaulis. These prosthecae greatly expand the cell surface to volume ratio and allow for increased nutrient uptake, providing the bacteria with a competitive advantage under nutrient limited conditions.

text: uncultured Anaerolineaceae bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR2113686_bin.17_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Wastewater

characteristic: environment (feature)

text: Wastewater

characteristic: environment (material)

text: Denmark

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: wastewater metagenome

text: wastewater metagenome

characteristic: metagenomic source

text: uncultured Armatimonadetes bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Armatimonadetes bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR2113686_bin.28_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Engineered

text: Wastewater

text: Wastewater

text: Denmark

text: metagenome-assembled genome

text: wastewater metagenome

text: wastewater metagenome

text: uncultured Bacteroidales bacterium

characteristic: organism

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR2113686_bin.34_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Wastewater

characteristic: environment (feature)

text: Wastewater

characteristic: environment (material)

text: Denmark

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: wastewater metagenome

text: wastewater metagenome

characteristic: metagenomic source

text: uncultured Thermoleophilia bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Thermoleophilia bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR2113686_bin.62_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR2113687 of study ERP024437.

characteristic: description

text: Engineered

text: Wastewater

text: Wastewater

text: Denmark

text: metagenome-assembled genome

text: wastewater metagenome

text: wastewater metagenome

text: Ruminococcus flavefaciens

characteristic: organism

text: Ruminococcus flavefaciens

text: Illumina MiSeq

text: multi-marker approach

text: Metagenome-assembled genome: ERR2113687_bin.13_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Multiple fragments where gaps span repetitive regions. Presence of the 23S, 16S, and 5S rRNA genes and at least 18 tRNAs

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Wastewater

characteristic: environment (feature)

text: Wastewater

characteristic: environment (material)

text: Denmark

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: wastewater metagenome

text: wastewater metagenome

characteristic: metagenomic source

text: Candidatus Fermentibacter daniensis

characteristic: project name

characteristic: sample derived from

text: Candidatus Fermentibacter daniensis

text: Illumina MiSeq

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR2113687_bin.41_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Engineered

text: Wastewater

text: Wastewater

text: Denmark

text: metagenome-assembled genome

text: wastewater metagenome

text: wastewater metagenome

text: uncultured bacterium

characteristic: organism

text: uncultured bacterium

text: Illumina MiSeq

text: multi-marker approach

text: Metagenome-assembled genome: ERR2113687_bin.60_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR2113688 of study ERP024437.

text: Engineered

characteristic: environment (biome)

text: Wastewater

characteristic: environment (feature)

text: Wastewater

characteristic: environment (material)

text: Denmark

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: wastewater metagenome

text: wastewater metagenome

characteristic: metagenomic source

text: uncultured bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured bacterium

text: Illumina MiSeq

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR2113688_bin.0_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Engineered

text: Wastewater

text: Wastewater

text: Denmark

text: metagenome-assembled genome

text: wastewater metagenome

text: wastewater metagenome

text: uncultured Acidobacteria bacterium

characteristic: organism

text: uncultured Acidobacteria bacterium

text: Illumina MiSeq

text: multi-marker approach

text: Metagenome-assembled genome: ERR2113688_bin.17_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Wastewater

characteristic: environment (feature)

text: Wastewater

characteristic: environment (material)

text: Denmark

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: wastewater metagenome

text: wastewater metagenome

characteristic: metagenomic source

text: uncultured Methanolinea sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Methanolinea sp.

text: Illumina MiSeq

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR2113688_bin.28_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Engineered

text: Wastewater

text: Wastewater

text: Denmark

text: metagenome-assembled genome

text: wastewater metagenome

text: wastewater metagenome

text: uncultured bacterium

characteristic: organism

text: uncultured bacterium

text: Illumina MiSeq

text: multi-marker approach

text: Metagenome-assembled genome: ERR2113688_bin.44_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Wastewater

characteristic: environment (feature)

text: Wastewater

characteristic: environment (material)

text: Denmark

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: wastewater metagenome

text: wastewater metagenome

characteristic: metagenomic source

text: uncultured bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured bacterium

text: Illumina MiSeq

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR2113688_bin.71_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.13.1

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR3519521 of study ERP117308.

characteristic: description

text: Engineered

text: Wastewater

text: Wastewater

text: United Kingdom

text: not provided

text: not provided

text: metagenome-assembled genome

text: wastewater metagenome

text: wastewater metagenome

text: uncultured Flavobacteriales bacterium

characteristic: organism

text: Hospitals are nodal points for antimicrobial consumption and identification of resistant pathogens and therefore hospital wastewater is a potential major source of AMR. This study uses metagenomics to study how the abundances of resistance genes in hospital wastewater are related to clinical activity within different buildings of the hospital. Sewage was collected over a 24-hour period from multiple wastewater collection points representing different specialties within a tertiary hospital site and simultaneously from community sewage works. High throughput shotgun sequencing was performed using Illumina HiSeq4000. Sequence reads were assigned taxonomically and to the AMR genes in the ResFinder database. The measured AMR gene abundances were then correlated to hospital antimicrobial usage (AMU), other data on clinical activity such as patient/ward characteristics and resistance prevalence in clinical isolates.

text: uncultured Flavobacteriales bacterium

text: Illumina HiSeq 4000

text: multi-marker approach

text: Metagenome-assembled genome: ERR3519521_bin.166_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.13.1

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Wastewater

characteristic: environment (feature)

text: Wastewater

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: wastewater metagenome

text: wastewater metagenome

characteristic: metagenomic source

text: Veillonella seminalis

characteristic: project name

characteristic: sample derived from

text: Veillonella seminalis

text: Illumina HiSeq 4000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR3519521_bin.172_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.13.1

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Engineered

text: Wastewater

text: Wastewater

text: United Kingdom

text: not provided

text: not provided

text: metagenome-assembled genome

text: wastewater metagenome

text: wastewater metagenome

text: Tepidiphilus thermophilus

characteristic: organism

text: Tepidiphilus thermophilus

text: Illumina HiSeq 4000

text: multi-marker approach

text: Metagenome-assembled genome: ERR3519521_bin.95_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.13.1

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Wastewater

characteristic: environment (feature)

text: Wastewater

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: wastewater metagenome

text: wastewater metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina HiSeq 4000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR3519521_bin.98_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.13.1

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR3519522 of study ERP117308.

characteristic: description

text: Engineered

text: Wastewater

text: Wastewater

text: United Kingdom

text: not provided

text: not provided

text: metagenome-assembled genome

text: wastewater metagenome

text: wastewater metagenome

text: uncultured Chitinophagaceae bacterium

characteristic: organism

text: uncultured Chitinophagaceae bacterium

text: Illumina HiSeq 4000

text: multi-marker approach

text: Metagenome-assembled genome: ERR3519522_bin.65_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.13.1

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Wastewater

characteristic: environment (feature)

text: Wastewater

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: wastewater metagenome

text: wastewater metagenome

characteristic: metagenomic source

text: uncultured Micropruina sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Micropruina sp.

text: Illumina HiSeq 4000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR3519522_bin.78_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.13.1

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR3519523 of study ERP117308.

characteristic: description

text: Engineered

text: Wastewater

text: Wastewater

text: United Kingdom

text: not provided

text: not provided

text: metagenome-assembled genome

text: wastewater metagenome

text: wastewater metagenome

text: uncultured Meiothermus sp.

characteristic: organism

text: uncultured Meiothermus sp.

text: Illumina HiSeq 4000

text: multi-marker approach

text: Metagenome-assembled genome: ERR3519523_bin.18_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes v3.13.1

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Wastewater

characteristic: environment (feature)

text: Wastewater

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: wastewater metagenome

text: wastewater metagenome

characteristic: metagenomic source

text: uncultured Leucobacter sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Leucobacter sp.

text: Illumina HiSeq 4000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR3519523_bin.22_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.13.1

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Engineered

text: Wastewater

text: Wastewater

text: United Kingdom

text: not provided

text: not provided

text: metagenome-assembled genome

text: wastewater metagenome

text: wastewater metagenome

text: uncultured Candidatus Saccharibacteria bacterium

characteristic: organism

text: Illumina HiSeq 4000

text: multi-marker approach

text: Metagenome-assembled genome: ERR3519523_bin.44_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.13.1

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Wastewater

characteristic: environment (feature)

text: Wastewater

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: wastewater metagenome

text: wastewater metagenome

characteristic: metagenomic source

text: Enterococcus aquimarinus

characteristic: project name

characteristic: sample derived from

text: Enterococcus aquimarinus

text: Illumina HiSeq 4000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR3519523_bin.63_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.13.1

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR3519524 of study ERP117308.

characteristic: description

text: Engineered

text: Wastewater

text: Wastewater

text: United Kingdom

text: not provided

text: not provided

text: metagenome-assembled genome

text: wastewater metagenome

text: wastewater metagenome

text: uncultured Pleomorphomonas sp.

characteristic: organism

text: uncultured Pleomorphomonas sp.

text: Illumina HiSeq 4000

text: multi-marker approach

text: Metagenome-assembled genome: ERR3519524_bin.105_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.13.1

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Wastewater

characteristic: environment (feature)

text: Wastewater

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: wastewater metagenome

text: wastewater metagenome

characteristic: metagenomic source

text: uncultured Alphaproteobacteria bacterium

text: Hospitals are nodal points for antimicrobial consumption and identification of resistant pathogens and therefore hospital wastewater is a potential major source of AMR. This study uses metagenomics to study how the abundances of resistance genes in hospital wastewater are related to clinical activity within different buildings of the hospital. Sewage was collected over a 24-hour period from multiple wastewater collection points representing different specialties within a tertiary hospital site and simultaneously from community sewage works. High throughput shotgun sequencing was performed using Illumina HiSeq4000. Sequence reads were assigned taxonomically and to the AMR genes in the ResFinder database. The measured AMR gene abundances were then correlated to hospital antimicrobial usage (AMU), other data on clinical activity such as patient/ward characteristics and resistance prevalence in clinical isolates.

characteristic: project name

characteristic: sample derived from

text: Illumina HiSeq 4000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR3519524_bin.59_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.13.1

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Engineered

text: Wastewater

text: Wastewater

text: United Kingdom

text: not provided

text: not provided

text: metagenome-assembled genome

text: wastewater metagenome

text: wastewater metagenome

text: uncultured Propioniciclava sp.

characteristic: organism

text: uncultured Propioniciclava sp.

text: Illumina HiSeq 4000

text: multi-marker approach

text: Metagenome-assembled genome: ERR3519524_bin.60_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.13.1

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR3519525 of study ERP117308.

text: Engineered

characteristic: environment (biome)

text: Wastewater

characteristic: environment (feature)

text: Wastewater

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: wastewater metagenome

text: wastewater metagenome

characteristic: metagenomic source

text: uncultured Lactobacillus sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Lactobacillus sp.

text: Illumina HiSeq 4000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR3519525_bin.44_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.13.1

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Engineered

text: Wastewater

text: Wastewater

text: United Kingdom

text: not provided

text: not provided

text: metagenome-assembled genome

text: wastewater metagenome

text: wastewater metagenome

text: Acinetobacter johnsonii

characteristic: organism

text: Acinetobacter johnsonii

text: Illumina HiSeq 4000

text: multi-marker approach

text: Metagenome-assembled genome: ERR3519525_bin.54_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.13.1

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Wastewater

characteristic: environment (feature)

text: Wastewater

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: wastewater metagenome

text: wastewater metagenome

characteristic: metagenomic source

text: uncultured Janthinobacterium sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Janthinobacterium sp.

text: Illumina HiSeq 4000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR3519525_bin.78_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.13.1

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Engineered

text: Wastewater

text: Wastewater

text: United Kingdom

text: not provided

text: not provided

text: metagenome-assembled genome

text: wastewater metagenome

text: wastewater metagenome

text: uncultured Propionibacteriaceae bacterium

characteristic: organism

text: Illumina HiSeq 4000

text: multi-marker approach

text: Metagenome-assembled genome: ERR3519525_bin.84_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes v3.13.1

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR3519526 of study ERP117308.

text: Engineered

characteristic: environment (biome)

text: Wastewater

characteristic: environment (feature)

text: Wastewater

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: wastewater metagenome

text: wastewater metagenome

characteristic: metagenomic source

text: uncultured Flavobacterium sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Flavobacterium sp.

text: Illumina HiSeq 4000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR3519526_bin.16_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.13.1

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Engineered

text: Wastewater

text: Wastewater

text: United Kingdom

text: not provided

text: not provided

text: metagenome-assembled genome

text: wastewater metagenome

text: wastewater metagenome

text: uncultured Clostridia bacterium

characteristic: organism

text: uncultured Clostridia bacterium

text: Illumina HiSeq 4000

text: multi-marker approach

text: Metagenome-assembled genome: ERR3519526_bin.62_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.13.1

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Wastewater

characteristic: environment (feature)

text: Wastewater

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: wastewater metagenome

text: wastewater metagenome

characteristic: metagenomic source

text: Aeromonas salmonicida

characteristic: project name

characteristic: sample derived from

text: Aeromonas salmonicida

text: Illumina HiSeq 4000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR3519526_bin.95_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.13.1

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR3519527 of study ERP117308.

characteristic: description

text: Engineered

text: Wastewater

text: Wastewater

text: United Kingdom

text: not provided

text: not provided

text: metagenome-assembled genome

text: wastewater metagenome

text: wastewater metagenome

text: uncultured Microbacterium sp.

characteristic: organism

text: uncultured Microbacterium sp.

text: Illumina HiSeq 4000

text: multi-marker approach

text: Metagenome-assembled genome: ERR3519527_bin.43_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.13.1

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR3519528 of study ERP117308.

text: Engineered

characteristic: environment (biome)

text: Wastewater

characteristic: environment (feature)

text: Wastewater

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: wastewater metagenome

text: wastewater metagenome

characteristic: metagenomic source

text: uncultured Burkholderiaceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Burkholderiaceae bacterium

text: Illumina HiSeq 4000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR3519528_bin.106_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.13.1

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Engineered

text: Wastewater

text: Wastewater

text: United Kingdom

text: not provided

text: not provided

text: metagenome-assembled genome

text: wastewater metagenome

text: wastewater metagenome

text: uncultured Burkholderiaceae bacterium

characteristic: organism

text: Hospitals are nodal points for antimicrobial consumption and identification of resistant pathogens and therefore hospital wastewater is a potential major source of AMR. This study uses metagenomics to study how the abundances of resistance genes in hospital wastewater are related to clinical activity within different buildings of the hospital. Sewage was collected over a 24-hour period from multiple wastewater collection points representing different specialties within a tertiary hospital site and simultaneously from community sewage works. High throughput shotgun sequencing was performed using Illumina HiSeq4000. Sequence reads were assigned taxonomically and to the AMR genes in the ResFinder database. The measured AMR gene abundances were then correlated to hospital antimicrobial usage (AMU), other data on clinical activity such as patient/ward characteristics and resistance prevalence in clinical isolates.

text: uncultured Burkholderiaceae bacterium

text: Illumina HiSeq 4000

text: multi-marker approach

text: Metagenome-assembled genome: ERR3519528_bin.13_CONCOCT_v1.1_MAG

characteristic: title