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text: Schizosaccharomyces pombe 972h-

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text: Schizosaccharomyces pombe 972h-

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text: A

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text: Schizosaccharomyces pombe 972h-

text: Schizosaccharomyces pombe 972h-

text: Schizosaccharomyces pombe 972h-


text: Infravec

text: public

text: Aedes Mariae

characteristic: common name

text: DNA barcoding (mtDNA COI gene sequencing) and genomic assembly of Aedes marinae and Aedes zammitii species. These species are part of the Aedes marinae complex distributed along the central-western Mediterranean coasts, and acting as vectors of the Plasmodium relictum (bird malaria).

characteristic: infravec request number

text: Acartomyia mariae

text: Acartomyia mariae

characteristic: study accession

text: Speciation and adaptation to saline habitats in the sea rock-pool mosquitoes Aedes mariae complex


text: Infravec

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Aedes�Zammitii

text: Acartomyia zammitii

text: Acartomyia zammitii


text: public

characteristic: SRA accession

tag: Namespace:A*STAR Institute of Molecular and Cell Biology

characteristic: Submitter Id

text: ArrayExpress

text: BJ

text: human

text: Protocols: Human iPSC-derived spinal cord organoids were collected as pools of 6 organoids at each timepoints. RNA were extracted by TRIzol followed by phase separation with chloroform. The aqueous phase were then purified with Qiagen RNeasy® Mini Kit. Directional mRNA library preparation with poly A enrichment.

text: neonate

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: spinal cord organoid culture in 1% alginate gel

characteristic: growth condition

text: Homo sapiens

text: Homo sapiens

text: skin of prepuce of penis

characteristic: organism part

text: induced pluripotent stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: male

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: A*STAR Institute of Molecular and Cell Biology

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: BJ

text: human

characteristic: description

text: neonate

text: normal

text: wild type genotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: skin of prepuce of penis

text: induced pluripotent stem cell

text: Homo sapiens

text: male

characteristic: title


characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: BJ

text: human

characteristic: common name

text: neonate

text: normal

text: wild type genotype

text: spinal cord organoid culture in 2% alginate gel

text: Homo sapiens

text: Homo sapiens

text: skin of prepuce of penis

text: induced pluripotent stem cell

text: Homo sapiens

text: male


text: public

text: ArrayExpress

text: BJ

text: human

text: neonate

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: spinal cord organoid culture in matrigel

characteristic: growth condition

text: Homo sapiens

text: Homo sapiens

text: skin of prepuce of penis

characteristic: organism part

text: induced pluripotent stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: male


text: female

characteristic: ArrayExpress-SEX

text: Streptococcus pneumoniae

characteristic: ArrayExpress-SPECIES

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Female

characteristic: GENDER

text: SC

characteristic: INSDC center name

text: public

text: Streptococcus pneumoniae

text: Streptococcus pneumoniae

text: Streptococcus pneumoniae


text: male

text: Streptococcus pneumoniae

text: Male

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Streptococcus pneumoniae

text: Streptococcus pneumoniae

characteristic: organism

text: Streptococcus pneumoniae

characteristic: title


text: Infravec

text: public

text: northern house mosquito

characteristic: common name

text: Transcriptome sequencing of diflubenzuron (DFB) insecticide resistant and susceptible Culex pipiens mosquitoes from Greece and Italy. This work focuses on assessing whether metabolic resistance due to the over expression of detoxifying genes (e.g cytochrome-P450s, glutathione-S-transferases, ABC transporters) co-occus in DFB resistant Culex pipiens mosquitoes.

characteristic: description

characteristic: infravec request number

text: Culex pipiens

text: Culex pipiens

characteristic: study accession

text: Identification of metabolic resistance mechanisms in diflubenzuron resistant Culex pipiens mosquitoes


characteristic: ENA-CHECKLIST

text: Qieenie

text: public

text: SPF barrier environment

characteristic: broad-scale environmental context

characteristic: collection date

text: house mouse

text: EFC020106 Mouse fecal sample

text: air

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Single cage

characteristic: local environmental context

text: Mus musculus

characteristic: project name

text: Mus musculus

text: Illumina NovaSeq

characteristic: sequencing method


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Qieenie

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: SPF barrier environment

text: house mouse

text: EFC020107 Mouse fecal sample

text: air

text: China

text: Single cage

text: Mus musculus

text: Mus musculus

text: Illumina NovaSeq


characteristic: ENA-CHECKLIST

text: Qieenie

text: public

text: SPF barrier environment

characteristic: broad-scale environmental context

characteristic: collection date

text: house mouse

characteristic: common name

text: EFC020108 Mouse fecal sample

characteristic: description

text: air

text: China

text: Single cage

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Illumina NovaSeq

characteristic: title


text: Qieenie

text: public

text: SPF barrier environment

text: house mouse

text: EFC020109 Mouse fecal sample

text: air

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Single cage

characteristic: local environmental context

text: Mus musculus

characteristic: project name

text: Mus musculus

text: Illumina NovaSeq

characteristic: sequencing method


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Qieenie

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: SPF barrier environment

text: house mouse

text: EFC020110 Mouse fecal sample

text: air

text: China

text: Single cage

text: Mus musculus

text: Mus musculus

text: Illumina NovaSeq


characteristic: ENA-CHECKLIST

text: Qieenie

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: SPF barrier environment

characteristic: broad-scale environmental context

characteristic: collection date

text: house mouse

characteristic: common name

text: EFC020111 Mouse fecal sample

characteristic: description

text: air

text: China

text: Single cage

text: Mus musculus

text: Mus musculus

text: Illumina NovaSeq


text: Qieenie

text: public

text: SPF barrier environment

text: house mouse

text: EFC020112 Mouse fecal sample

text: air

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Single cage

characteristic: local environmental context

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Illumina NovaSeq

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Qieenie

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: SPF barrier environment

text: house mouse

text: EFC020113 Mouse fecal sample

text: air

text: China

text: Single cage

text: Mus musculus

characteristic: project name

text: Mus musculus

text: Illumina NovaSeq

characteristic: sequencing method


characteristic: ENA-CHECKLIST

text: Qieenie

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: SPF barrier environment

characteristic: broad-scale environmental context

characteristic: collection date

text: house mouse

text: EFC020114 Mouse fecal sample

text: air

text: China

text: Single cage

text: Mus musculus

text: Mus musculus

text: Illumina NovaSeq


text: Qieenie

text: public

text: SPF barrier environment

text: house mouse

characteristic: common name

text: EFC020115 Mouse fecal sample

characteristic: description

text: air

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Single cage

characteristic: local environmental context

text: Mus musculus

text: Mus musculus

text: Illumina NovaSeq


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Qieenie

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: SPF barrier environment

text: house mouse

text: EFC020116 Mouse fecal sample

text: air

text: China

text: Single cage

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Illumina NovaSeq

characteristic: title


characteristic: ENA-CHECKLIST

text: Qieenie

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: SPF barrier environment

text: house mouse

text: EFC020117 Mouse fecal sample

text: air

text: China

text: Single cage

text: Mus musculus

characteristic: project name

text: Mus musculus

text: Illumina NovaSeq

characteristic: sequencing method


text: UNIVERSITY OF PERUGIA

text: public

tag: Namespace:UNIVERSITY OF PERUGIA

text: human

characteristic: common name

text: AML cell lines

characteristic: description

text: Homo sapiens

text: Homo sapiens


text: Infravec

text: public

text: castor bean tick

text: Characterisation of the viral communities present in Ixodes ricinus ticks from Portugal with the aim of developing PCR primers for the screening of specific bacterial and viral species.

characteristic: infravec request number

text: Ixodes ricinus

text: Ixodes ricinus

characteristic: study accession

text: Metagenomic profiling of the viral and bacterial communities in Ixodes ricinus from Portugal


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: NATIONAL VETERINARY INSTITUTE (SVA)

text: public

tag: Namespace:NATIONAL VETERINARY INSTITUTE (SVA)

text: E. coli from Swedish wheat flour

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: isolate

text: Wheat flour

text: Escherichia coli

text: Escherichia coli

text: E. coli isolate 21-MIK138561


characteristic: External Id

text: NATIONAL VETERINARY INSTITUTE (SVA)

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E. coli from Swedish wheat flour

text: Sweden

text: Wheat flour

text: Escherichia coli

characteristic: organism

text: Escherichia coli

text: E. coli isolate 21-MIK138611


characteristic: ENA-CHECKLIST

text: NATIONAL VETERINARY INSTITUTE (SVA)

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E. coli from Swedish wheat flour

text: Sweden

text: Wheat flour

text: Escherichia coli

text: Escherichia coli

text: E. coli isolate 21-MIK138636

characteristic: title


text: NATIONAL VETERINARY INSTITUTE (SVA)

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: E. coli from Swedish wheat flour

text: Sweden

text: Wheat flour

text: Escherichia coli

text: Escherichia coli

text: E. coli isolate 21-MIK138645


text: NATIONAL VETERINARY INSTITUTE (SVA)

text: public

text: E. coli from Swedish wheat flour

characteristic: description

text: Sweden

text: Wheat flour

text: Escherichia coli

text: Escherichia coli

text: E. coli isolate 21-MIK138647


text: NATIONAL VETERINARY INSTITUTE (SVA)

text: public

text: E. coli from Swedish wheat flour

text: Sweden

text: Wheat flour

text: Escherichia coli

text: Escherichia coli

text: E. coli isolate 21-MIK138664


text: NATIONAL VETERINARY INSTITUTE (SVA)

text: public

text: E. coli from Swedish wheat flour

text: Sweden

text: Wheat flour

text: Escherichia coli

text: Escherichia coli

text: E. coli isolate 21-MIK138684


text: NATIONAL VETERINARY INSTITUTE (SVA)

text: public

text: E. coli from Swedish wheat flour

text: Sweden

text: Wheat flour

text: Escherichia coli

text: Escherichia coli

text: E. coli isolate 21-MIK138717


text: NATIONAL VETERINARY INSTITUTE (SVA)

text: public

text: E. coli from Swedish wheat flour

text: Sweden

text: Wheat flour

text: Escherichia coli

text: Escherichia coli

text: E. coli isolate 21-MIK138728


text: NATIONAL VETERINARY INSTITUTE (SVA)

text: public

text: E. coli from Swedish wheat flour

text: Sweden

text: Wheat flour

text: Escherichia coli

text: Escherichia coli

text: E. coli isolate 21-MIK138733


text: UoH

text: public

text: 5 to 6

unit: week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: golden hamster

characteristic: common name

text: Protocols: HapT1 cell line described in this publication DOI: 10.18632/oncotarget.9729 Thin needle biospies were taken from PDAC tumours on the day before the last injection. Biopsies were placed in RNA-later for extraction. 5-6 week old Syrian hamsters were engrafted with HapT1 cells grown in culture. The cells were grown (3x passages) in RPMI (10%FBS, 1%L-Glut, 1% P/s) until 80% confluency and were washed three times before engraftment. Hamsters were intraperitoneally injected with either 300ug of IgG2a or anti-PD-L1 every three days for a total of 8 injections. After extraction of gene hit counts, the gene hit counts table was used for downstream differential expression analysis. Using DESeq2, a comparison of gene expression between the customer-defined groups of samples was performed. The Wald test was used to generate p-values and log2 fold changes. Genes with an adjusted p-value < 0.05 and absolute log2 fold change > 1 were called as differentially expressed genes for each comparison. Below are the results of the number of significantly differentially expressed genes for all comparisons provided. RNAeasy mini kit (qiagen) was used according to the manufacturers instructions PolyA selection

text: pancreatic ductal adenocarcinoma

characteristic: disease

text: wild type genotype

characteristic: genotype

text: Mesocricetus auratus

text: Mesocricetus auratus

text: pancreas

characteristic: organism part

text: neoplasm

characteristic: sampling site

text: Mesocricetus auratus

text: male

characteristic: sex

text: HsdHan®:AURA

characteristic: strain

text: 5 x10E+6 HapT1 cells into left flank until neoplasm formation

characteristic: xenograft


KE

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: KE

tag: Namespace:Hungarian University of Agriculture and Life Sciences

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: collection date

unit: m

characteristic: depth

text: Kengyel

text: soil

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: freshwater biome

characteristic: local environmental context

text: uncultured bacterium

characteristic: organism

text: BOKU

characteristic: project name

text: uncultured bacterium

text: MiSeq

characteristic: sequencing method


KA

characteristic: ENA-CHECKLIST

text: Hungarian University of Agriculture and Life Sciences

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: KA

characteristic: Submitter Id

text: aquatic biome

text: Karcag

characteristic: description

text: soil

text: Hungary

text: freshwater biome

text: uncultured bacterium

text: BOKU

text: uncultured bacterium

text: MiSeq

characteristic: title


RA

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: RA

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: collection date

text: Rakoczifalva

text: soil

text: Hungary

text: freshwater biome

text: uncultured bacterium

text: BOKU

text: uncultured bacterium

text: MiSeq


TH

text: public

text: TH

text: aquatic biome

characteristic: depth

text: Tisza-H

text: soil

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: freshwater biome

characteristic: local environmental context

text: uncultured bacterium

characteristic: organism

text: BOKU

text: uncultured bacterium

text: MiSeq


SZ

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: SZ

characteristic: Submitter Id

text: aquatic biome

text: Szolnok

text: soil

text: Hungary

text: freshwater biome

text: uncultured bacterium

text: BOKU

characteristic: project name

text: uncultured bacterium

text: MiSeq

characteristic: sequencing method


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Shanghai Tenth People's Hospital

characteristic: INSDC center name

characteristic: INSDC first public

text: public

tag: Namespace:Shanghai Tenth People's Hospital

text: ArrayExpress

characteristic: broker name

text: Protocols: Cells were grown in YPD broth at 37˚C from OD600=0.01 till OD600=1.0. Cells were treated with 0.06 µg/ml or 0.5 µg/ml of tunicamycin, or DMSO (control) for 1h. Pellets of 5ml cell culture were obtained by centrifuging at maximum speed for 1min. the pellets were fast frozen in liquid nitrogen. RNA was extracted using the TRIzol method following the manufacturer's protocol. Cell pellets were transferred from liquid nitrogen into the 2ml tub containing 1.5ml Trizol reagent. The mix was shared for 3min, and kept for 5in at room temperature, then centrifuged at 10,000xg for 5min at 4˚C. The supernatant was added 200µl of chloroform/isoamyl alcohol (24:1) with 1ml lysis reagents. After centrifuged at 10,000g for 10min at 4˚C, the supernatant was transferred into another new tube with equal volume of isopropanol and put in the refrigerator at -20˚C for 1h. After centrifuged at 13600xg for 20min at 4˚C, the supernatant was precipitated by 1ml of 75% ethanol and let dry for 3-5min. The RNA pellet was dissolved with 30-100µl of DEPC water. The concentration of the extracted RNA was determined using a Nanodrop system (NanoDrop, Madison, USA), and the integrity of the RNA was examined by the RNA integrity number (RIN) using an Agilent 2100 bioanalyzer (Agilent, Santa Clara, USA) buffer at appropriate temperature. Then First-strand cDNA was generated using random hexamer-primed reverse transcription, followed by a second-strand cDNA synthesis. afterwards, A-Tailing Mix and RNA Index Adapters were added by incubating to end repair. The cDNA fragments obtained from previous step were amplified by PCR, and products were purified by Ampure XP Beads, then dissolved in EB solution. The product was validated on the Agilent Technologies 2100 bioanalyzer for quality control. The double stranded PCR products from previous step were heated denatured and circularized by the splint oligo sequence to get the final library. The single strand circle DNA (ssCir DNA) was formatted as the final library.

characteristic: description

text: wild type genotype

characteristic: genotype

text: YPD medium

characteristic: growth condition

text: Cryptococcus neoformans var. grubii H99

text: Cryptococcus neoformans var. grubii H99

text: whole organism

characteristic: organism part

text: Cryptococcus neoformans var. grubii H99

characteristic: title


name: E-MTAB-117041666018888:F Exconjugant #1 corrected

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

tag: Namespace:Institute for Integrative Biology of the Cell (I2BC)

text: ArrayExpress

text: Protocols: ~210 000 colonies were scraped from the LB agar plate containing appropriate antibiotics total gDNA was purified by GenElute Bacterial Genomic DNA kit (Sigma-Aldrich, St. Louis, MO) Library preparation was carried out as described by Espinosa et al. (https://doi.org/10.1016/j.xpro.2020.100202). In brief, DNA was first digested with MmeI. Fragment containing Tn was size-fractioned and then adaptor-ligated. PCR was carried out to add appropriate adapters while amplify the target. Size-fractioned and purified PCR product was subjected to sequencing.

text: MC1061::Tn / F

characteristic: individual

text: Escherichia coli K-12

characteristic: organism

text: Escherichia coli K-12

text: Escherichia coli K-12

text: F Exconjugant #1 corrected


name: E-MTAB-117041666018888:pESBL Exconjugant #1 corrected

text: Institute for Integrative Biology of the Cell (I2BC)

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: MC1061::Tn / pESBL

text: Escherichia coli K-12

text: Escherichia coli K-12

text: Escherichia coli K-12

text: pESBL Exconjugant #1 corrected


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

tag: Namespace:1. Institut Pasteur, Universite Paris Cite, Plate-forme Technologique Biomics, F-75015 Paris, France    2. Institut Pasteur, Universite Paris Cite, Bioinformatics and Biostatistics Hub, F-75015 Paris, France

text: ArrayExpress

text: TMC 102

text: Protocols: To isolate resistant mutants, exponentially growing cultures were concentrated, and about 1010 cells were plated onto 7H10 agar plates, containing different concentrations of a given drug, ranging from 4X- to 20X-MIC. Plates were incubated at 37 °C for 4 weeks. The resistant phenotype was validated by assessing the MIC at least twice on the isolated colonies. Resistant colonies were reinoculated in the presence of the compound (8X-MIC) and 10 mL of exponentially growing cultures were used for genomic DNA isolation. In short, each cell pellet was heat-inactivated at 80 ˚C for 1, washed and resuspended in 450 µL of bug lysis solution (25 mM Tris-HCl pH 7.9; 10 mM EDTA 50 mM glucose). 50 µL of lysozyme (10 mg/mL) were added and the cell suspension was incubated 37 ˚C overnight. Next, 100 µL of sodium dodecyl sulfate (10 %) and 50 µL of proteinase K (10 mg/mL) were added, mixed gently by inversion, and incubated at 55 ˚C for 30 min. Next, 200 µL of sodium chloride (5 M) were added and mixed gently by inversion, followed by 160 µL of preheated cetrimide saline solution (4 %), mixed and incubated at 65˚C for 10 min. Genomic DNA was extracted twice, adding equal volume (1 mL) of chloroform: isoamyl alcohol (24:1), mixing by inversion, and centrifuging for 5 min at 12000 RPM. The aqueous layer was transferred to a clean tube (800 µL) and 560 µL (0.7X volumes) of isopropanol were added at room temperature, mixed by inversion until DNA precipitation. DNA was collected by centrifugation for 10 min and the pellet was washed with 70 % ethanol, dried and resuspended in 100 µL of TE buffer. Libraries were built using a TruSeq DNA PCR-Free Low Throughput Library Prep Kit (Illumina, USA) following the manufacturer’s protocol. Quality control was performed on an Agilent BioAnalyzer.

text: wild type genotype

text: logarithmic phase culture

text: Mycobacterium tuberculosis H37Rv

text: Mycobacterium tuberculosis H37Rv

text: whole organism

text: Mycobacterium tuberculosis H37Rv


characteristic: External Id

text: 1. Institut Pasteur, Universite Paris Cite, Plate-forme Technologique Biomics, F-75015 Paris, France 2. Institut Pasteur, Universite Paris Cite, Bioinformatics and Biostatistics Hub, F-75015 Paris, France

characteristic: INSDC center name

text: public

text: ArrayExpress

text: TMC 102

text: wild type genotype

text: logarithmic phase culture

text: Mycobacterium tuberculosis H37Rv

text: Mycobacterium tuberculosis H37Rv

text: whole organism

text: Mycobacterium tuberculosis H37Rv


characteristic: INSDC first public

text: public

text: ArrayExpress

characteristic: broker name

text: TMC 102

text: Protocols: Primary cultures were grown up to OD600nm 0.5/0.6 and diluted to OD600nm 0.025 using pre-warmed Middlebrook 7H9 broth. Secondary cultures were grown up to OD600nm 0.25 and drugs were added at a concentration equal to 10 fold the minimal inhibitory concentration for 6 hours. Cells were collected by centrifugation for 10 minutes and frozen at -80 °C, before RNA isolation. RNA was extracted by the hot phenol method. In detail, bacterial cell pellets were processed in 400 µL of hot lysis buffer (50 mM Tris pH 8.0, 1 mM EDTA, 1% SDS) and then mixed in another screw cap tube containing 400 µL of hot phenol (pH 6.6) and 400 µL of acidified glass beads (425-600-µm diameter). Cells were incubated at 65 °C for 15 minutes with intermittent vortexing every 5 minutes. Samples were centrifuged at 10,000 rpm for 15 minutes at 4 °C. The aqueous phase was separated into another fresh screw-capped microcentrifuge tube, an equal amount of Trizol and 100 µl of chloroform were added and mixed gently by inversion. The samples were again centrifuged at 10,000 rpm for 10 minutes at 4 °C, the aqueous phase separated into a fresh tube, and the RNA was precipitated with 0.5 M LiCl and three volumes of cold 100% ethanol. Samples were mixed gently by inversion, and then precipitated for 2 hours at -20 °C. Samples were centrifuged at 13,500 rpm for 35 minutes at 4 °C and RNA was washed with 80% ethanol. RNA samples were air-dried for 30 minutes and resuspended in 20 µL of nuclease-free water. Finally, DNA was depleted by TURBO DNase treatment (Ambion), according to the manufacturer's instructions. The RNA integrity index was examined on the Agilent Bioanalyzer RNA 6000 Pico kit to confirm the quality and integrity of the RNA for RNAseq purposes. For RTq-PCR, the concentration was measured via NanoDrop 2000/2000c spectrophotometer. rRNA was depleted using the QIAseq FastSelect 5S/16S/23S kit for bacteria (Qiagen),Ribosoma vailableaccording to manufacturer’s recommendations. The libraries were prepared using the TruSeq Stranded mRNA Library Preparation kit (Illumina) according to manufacturer’s protocol https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseq-stranded-mrna-workflow/truseq-stranded-mrna-workflow-checklist-1000000040600-00.pdf. The quality, integrity and amount of the libraries were assayed with the Agilent 2100 Bioanalyzer and Qubit fluorimeter.

text: wild type genotype

text: logarithmic phase culture

text: Mycobacterium tuberculosis H37Rv

text: Mycobacterium tuberculosis H37Rv

text: whole organism

text: Mycobacterium tuberculosis H37Rv


text: public

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:172-2926

tag: Namespace:FRIEDRICH-LOEFFLER-INSTITUT (FLI) Institute for Bacterial Infections and Zoonoses (IBIZ)

characteristic: collection date

text: isolate

characteristic: description

text: No

text: Greece

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: geographic location (region and locality)

text: not collected

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

characteristic: isolate

text: Blood

text: Brucella melitensis

text: Brucella melitensis

characteristic: strain


characteristic: ENA-CHECKLIST

text: FRIEDRICH-LOEFFLER-INSTITUT (FLI) Institute for Bacterial Infections and Zoonoses (IBIZ)

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:172-2927

text: isolate

text: No

text: Greece

text: not collected

text: not collected

text: Homo sapiens

text: Blood

text: Brucella melitensis

characteristic: organism

text: Brucella melitensis

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:172-2928

characteristic: Submitter Id

text: isolate

text: No

text: Greece

text: not collected

text: not collected

text: Homo sapiens

text: Blood

text: Brucella melitensis

text: Brucella melitensis


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:172-2929

characteristic: collection date

text: isolate

characteristic: description

text: No

text: Greece

text: not collected

text: not collected

text: Homo sapiens

text: Blood

text: Brucella melitensis

text: Brucella melitensis


text: public

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:172-2930

text: isolate

text: No

text: Greece

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: geographic location (region and locality)

text: not collected

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: Blood

text: Brucella melitensis

text: Brucella melitensis


characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:172-2931

text: isolate

text: No

text: Greece

text: not collected

text: not collected

text: Homo sapiens

characteristic: isolate

text: Blood

text: Brucella melitensis

text: Brucella melitensis

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:172-2932

text: isolate

text: No

text: Greece

text: not collected

text: not collected

text: Homo sapiens

text: Blood

text: Brucella melitensis

characteristic: organism

text: Brucella melitensis

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:173-2933

characteristic: Submitter Id

text: isolate

text: No

text: Greece

text: not collected

text: not collected

text: Homo sapiens

text: Blood

text: Brucella melitensis

text: Brucella melitensis


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:173-2934

characteristic: collection date

text: isolate

characteristic: description

text: No

text: Greece

text: not collected

text: not collected

text: Homo sapiens

text: Blood

text: Brucella melitensis

text: Brucella melitensis


text: public

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:173-2935

text: isolate

text: No

text: Greece

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: geographic location (region and locality)

text: not collected

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: Blood

text: Brucella melitensis

text: Brucella melitensis


characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:173-2936

text: isolate

text: No

text: Greece

text: not collected

text: not collected

text: Homo sapiens

characteristic: isolate

text: Blood

text: Brucella melitensis

text: Brucella melitensis

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:173-2937

text: isolate

text: No

text: Greece

text: not collected

text: not collected

text: Homo sapiens

text: Blood

text: Brucella melitensis

characteristic: organism

text: Brucella melitensis

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:173-2938

characteristic: Submitter Id

text: isolate

text: No

text: Greece

text: not collected

text: not collected

text: Homo sapiens

text: Blood

text: Brucella melitensis

text: Brucella melitensis


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:173-2939

characteristic: collection date

text: isolate

characteristic: description

text: No

text: Greece

text: not collected

text: not collected

text: Homo sapiens

text: Blood

text: Brucella melitensis

text: Brucella melitensis


text: public

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:173-2940

text: isolate

text: No

text: Greece

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: geographic location (region and locality)

text: not collected

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: CFS

text: Brucella melitensis

text: Brucella melitensis


characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:173-2941

text: not collected

text: isolate

text: No

text: Greece

text: not collected

text: not collected

text: Ovis aries

characteristic: isolate

text: Liver

text: Brucella melitensis

text: Brucella melitensis

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:173-2942

text: not collected

text: isolate

text: No

text: Greece

text: not collected

text: not collected

text: Capra aegagrus hircus

text: Liver

text: Brucella melitensis

characteristic: organism

text: Brucella melitensis

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: FRIEDRICH-LOEFFLER-INSTITUT (FLI) Institute for Bacterial Infections and Zoonoses (IBIZ)

text: public

characteristic: SRA accession

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:173-2943

characteristic: Submitter Id

text: not collected

text: isolate

text: No

text: Greece

text: not collected

text: not collected

text: Ovis aries

text: no data

text: Brucella melitensis

text: Brucella melitensis


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:173-2944

text: not collected

characteristic: collection date

text: isolate

characteristic: description

text: No

text: Greece

text: not collected

text: not collected

text: Ovis aries

text: Spleen

text: Brucella melitensis

text: Brucella melitensis


text: public

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:173-2945

text: not collected

text: isolate

text: No

text: Greece

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: geographic location (region and locality)

text: not collected

characteristic: host health state

text: Ovis aries

characteristic: host scientific name

text: no data

text: Brucella melitensis

text: Brucella melitensis


characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:173-2946

text: not collected

text: isolate

text: No

text: Greece

text: not collected

text: not collected

text: Capra aegagrus hircus

characteristic: isolate

text: Liver

text: Brucella melitensis

text: Brucella melitensis

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:173-2947

text: not collected

text: isolate

text: No

text: Greece

text: not collected

text: not collected

text: Ovis aries

text: Liver

text: Brucella melitensis

characteristic: organism

text: Brucella melitensis

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:173-2948

characteristic: Submitter Id

text: not collected

text: isolate

text: No

text: Greece

text: not collected

text: not collected

text: Ovis aries

text: no data

text: Brucella melitensis

text: Brucella melitensis


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:174-2949

text: not collected

characteristic: collection date

text: isolate

characteristic: description

text: No

text: Greece

text: not collected

text: not collected

text: Ovis aries

text: Liver

text: Brucella melitensis

text: Brucella melitensis


text: public

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:174-2950

text: not collected

text: isolate

text: No

text: Greece

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: geographic location (region and locality)

text: not collected

characteristic: host health state

text: Ovis aries

characteristic: host scientific name

text: Fetus

text: Brucella melitensis

text: Brucella melitensis


characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:174-2951

text: not collected

text: isolate

text: No

text: Greece

text: not collected

text: not collected

text: Ovis aries

characteristic: isolate

text: no data

text: Brucella melitensis

text: Brucella melitensis

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:174-2952

text: not collected

text: isolate

text: No

text: Greece

text: not collected

text: not collected

text: Ovis aries

text: no data

text: Brucella melitensis

characteristic: organism

text: Brucella melitensis

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:174-2953

characteristic: Submitter Id

text: not collected

text: isolate

text: No

text: Greece

text: not collected

text: not collected

text: Ovis aries

text: no data

text: Brucella melitensis

text: Brucella melitensis


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:174-2954

text: not collected

characteristic: collection date

text: isolate

characteristic: description

text: No

text: Greece

text: not collected

text: not collected

text: Ovis aries

text: no data

text: Brucella melitensis

text: Brucella melitensis


text: public

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:174-2955

text: not collected

text: isolate

text: No

text: Greece

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: geographic location (region and locality)

text: not collected

characteristic: host health state

text: Ovis aries

characteristic: host scientific name

text: no data

text: Brucella melitensis

text: Brucella melitensis


characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:174-2956

text: not collected

text: isolate

text: No

text: Greece

text: not collected

text: not collected

text: Ovis aries

characteristic: isolate

text: no data

text: Brucella melitensis

text: Brucella melitensis

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:174-2957

text: not collected

text: isolate

text: No

text: Greece

text: not collected

text: not collected

text: Ovis aries

text: no data

text: Brucella melitensis

characteristic: organism

text: Brucella melitensis

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:174-2958

characteristic: Submitter Id

text: not collected

text: isolate

text: No

text: Greece

text: not collected

text: not collected

text: Ovis aries

text: no data

text: Brucella melitensis

text: Brucella melitensis


text: FRIEDRICH-LOEFFLER-INSTITUT (FLI) Institute for Bacterial Infections and Zoonoses (IBIZ)

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:174-2959

text: not collected

characteristic: collection date

text: isolate

characteristic: description

text: No

text: Greece

text: not collected

text: not collected

text: Ovis aries

text: no data

text: Brucella melitensis

text: Brucella melitensis


text: public

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:174-2960

text: not collected

text: isolate

text: No

text: Greece

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: geographic location (region and locality)

text: not collected

characteristic: host health state

text: Ovis aries

characteristic: host scientific name

text: no data

text: Brucella melitensis

text: Brucella melitensis


characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:174-2961

text: not collected

text: isolate

text: No

text: Greece

text: not collected

text: not collected

text: Ovis aries

characteristic: isolate

text: no data

text: Brucella melitensis

text: Brucella melitensis

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:174-2962

text: not collected

text: isolate

text: No

text: Greece

text: not collected

text: not collected

text: Ovis aries

text: no data

text: Brucella melitensis

characteristic: organism

text: Brucella melitensis

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:174-2963

characteristic: Submitter Id

text: not collected

text: isolate

text: No

text: Greece

text: not collected

text: not collected

text: Ovis aries

text: no data

text: Brucella melitensis

text: Brucella melitensis


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:174-2964

text: not collected

characteristic: collection date

text: isolate

characteristic: description

text: No

text: Greece

text: not collected

text: not collected

text: Ovis aries

text: no data

text: Brucella melitensis

text: Brucella melitensis


text: public

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:175-2965

text: not collected

text: isolate

text: No

text: Greece

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: geographic location (region and locality)

text: not collected

characteristic: host health state

text: Ovis aries

characteristic: host scientific name

text: no data

text: Brucella melitensis

text: Brucella melitensis


characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:175-2966

text: not collected

text: isolate

text: No

text: Greece

text: not collected

text: not collected

text: Ovis aries

characteristic: isolate

text: no data

text: Brucella melitensis

text: Brucella melitensis

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:175-2967

text: not collected

text: isolate

text: No

text: Greece

text: not collected

text: not collected

text: Ovis aries

text: no data

text: Brucella melitensis

characteristic: organism

text: Brucella melitensis

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:175-2968

characteristic: Submitter Id

text: not collected

text: isolate

text: No

text: Greece

text: not collected

text: not collected

text: Ovis aries

text: no data

text: Brucella melitensis

text: Brucella melitensis


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ena-SAMPLE-TAB-18-10-2022-07:16:33:175-2969

text: not collected

characteristic: collection date

text: isolate

characteristic: description

text: No

text: Greece

text: not collected

text: not collected

text: Ovis aries

text: no data

text: Brucella abortus

text: Brucella abortus


C

text: WAGENINGEN UNIVERSIT

text: public

text: C

tag: Namespace:WAGENINGEN UNIVERSIT

text: potato

characteristic: common name

text: Solanum tuberosum clone C (USW5337.3)

characteristic: estimated size

text: C

characteristic: genotype

text: Netherlands

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: isolation and growth condition

characteristic: number of replicons

text: Solanum tuberosum

text: potato

characteristic: organism common name

characteristic: plant developmental stage

text: soil

characteristic: plant growth medium

characteristic: plant structure

characteristic: ploidy

text: Solanum tuberosum

text: Potato clone C


E

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: WAGENINGEN UNIVERSIT

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E

characteristic: Submitter Id

text: potato

text: Solanum tuberosum clone E (77.2102.37)

text: E

text: Netherlands

text: Solanum tuberosum

characteristic: organism

text: potato

text: soil

text: Solanum tuberosum

text: Potato clone E

characteristic: title


text: WAGENINGEN UNIVERSIT

characteristic: INSDC first public

text: public

characteristic: collection date

text: potato

characteristic: common name

text: Solanum tuberosum C x E offspring genotype

characteristic: description

characteristic: estimated size

characteristic: genotype

text: Netherlands

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: isolation and growth condition

characteristic: number of replicons

text: Solanum tuberosum

text: potato

text: soil

text: Solanum tuberosum

text: Potato CxE offspring


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: ena-upload-cli

tag: Namespace:Clinical Bioinformatics Area

text: Servicio de Microbiologia, Hospital Universitario Virgen del Rocio, Sevilla, Spain

characteristic: collecting institution

text: not provided

characteristic: collector name

text: Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region. Sequencing supported by RELECOV consortium, funded by the European Centre for Diseases Prevention and Control (ECDC) under Grant Agreement ECDC/HERA/2021/024 ECDC.12241

text: Spain

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: active surveillance in response to outbreak

characteristic: sample capture status

text: AND23717, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


text: Clinical Bioinformatics Area

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collection date

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

text: AND24168, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: ena-upload-cli

characteristic: collecting institution

text: not provided

characteristic: collector name

text: Spain

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND24169, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


text: Clinical Bioinformatics Area

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collection date

text: not provided

text: Spain

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status

text: AND24170, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

text: public

text: ena-upload-cli

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

text: AND24171, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

text: Servicio de Microbiologia, Hospital Juan Ramon Jimenez, Huelva, Spain

characteristic: collecting institution

text: not provided

characteristic: collector name

text: Spain

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND24172, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

text: public

text: ena-upload-cli

characteristic: collection date

text: not provided

text: Spain

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status

text: AND24173, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-upload-cli

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

text: AND24174, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

text: public

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collecting institution

text: not provided

characteristic: collector name

text: Spain

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND24175, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-upload-cli

characteristic: collection date

text: not provided

text: Spain

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status

text: AND24176, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

text: public

text: ena-upload-cli

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

text: AND24177, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collecting institution

text: not provided

characteristic: collector name

text: Spain

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND24178, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

text: public

text: ena-upload-cli

characteristic: collection date

text: not provided

text: Spain

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status

text: AND24180, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-upload-cli

text: Servicio de Microbiologia, Hospital Universitario Virgen del Rocio, Sevilla, Spain

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

text: AND24181, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

text: public

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collecting institution

text: not provided

characteristic: collector name

text: Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region. Sequencing supported by RELECOV consortium, funded by the European Centre for Diseases Prevention and Control (ECDC) under Grant Agreement ECDC/HERA/2021/024 ECDC.12241

text: Spain

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: active surveillance in response to outbreak

text: AND24182, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-upload-cli

characteristic: collection date

text: not provided

text: Spain

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status

text: AND24183, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

text: public

text: ena-upload-cli

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

text: AND24184, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collecting institution

text: not provided

characteristic: collector name

text: Spain

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND24185, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

text: public

text: ena-upload-cli

characteristic: collection date

text: not provided

text: Spain

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status

text: AND24186, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-upload-cli

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

text: AND24187, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

text: public

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collecting institution

text: not provided

characteristic: collector name

text: Spain

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND24188, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-upload-cli

characteristic: collection date

text: not provided

text: Spain

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status

text: AND24189, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

text: public

text: ena-upload-cli

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

text: AND24190, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collecting institution

text: not provided

characteristic: collector name

text: Spain

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND24191, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

text: public

text: ena-upload-cli

characteristic: collection date

text: not provided

text: Spain

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status

text: AND24192, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-upload-cli

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

text: AND24193, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

text: public

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collecting institution

text: not provided

characteristic: collector name

text: Spain

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND24194, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-upload-cli

text: Servicio de Microbiologia, Hospital Universitario Virgen del Rocio, Sevilla, Spain

characteristic: collection date

text: not provided

text: Spain

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status

text: AND24195, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

text: public

text: ena-upload-cli

text: not provided

text: Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region. Sequencing supported by RELECOV consortium, funded by the European Centre for Diseases Prevention and Control (ECDC) under Grant Agreement ECDC/HERA/2021/024 ECDC.12241

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

text: AND24196, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collecting institution

text: not provided

characteristic: collector name

text: Spain

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: active surveillance in response to outbreak

text: AND24197, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

text: public

text: ena-upload-cli

characteristic: collection date

text: not provided

text: Spain

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status

text: AND24198, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-upload-cli

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

text: AND24199, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

text: public

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collecting institution

text: not provided

characteristic: collector name

text: Spain

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND24200, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-upload-cli

characteristic: collection date

text: not provided

text: Spain

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status

text: AND24201, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

text: public

text: ena-upload-cli

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

text: AND24202, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collecting institution

text: not provided

characteristic: collector name

text: Spain

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND24203, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

text: public

text: ena-upload-cli

characteristic: collection date

text: not provided

text: Spain

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status

text: AND24204, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-upload-cli

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

text: AND24205, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

text: public

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collecting institution

text: not provided

characteristic: collector name

text: Spain

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND24206, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region