characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: not provided

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR15730543 of study SRP335854.

text: feces

characteristic: environmental medium

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Prevotella sp.

characteristic: organism

text: We performed a deep whole metagenomic shotgun sequencing on mouse samples. Metagenomic de-novo assembly was performed on the samples.

characteristic: project name

text: uncultured Prevotella sp.

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Prevotellaceae;g__Prevotella;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR15730543_bin.13_metawrap_v1.3_MAG

characteristic: title


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: not provided

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: feces

text: not provided

text: not provided

unit: DD

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

characteristic: metagenomic source

text: Faecalibaculum rodentium

characteristic: sample derived from

text: Faecalibaculum rodentium

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Erysipelotrichia;o__Erysipelotrichales;f__Erysipelotrichaceae;g__Faecalibaculum;s__Faecalibaculum rodentium

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR15730543_bin.44_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: not provided

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

text: CheckM

text: feces

characteristic: environmental medium

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Eubacteriaceae bacterium

characteristic: organism

characteristic: project name

text: uncultured Eubacteriaceae bacterium

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Eubacteriaceae;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR15730543_bin.86_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR15730547 of study SRP335854.

characteristic: description

text: feces

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Roseburia sp.

characteristic: sample derived from

text: uncultured Roseburia sp.

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Roseburia;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR15730547_bin.23_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: not provided

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR15730548 of study SRP335854.

text: feces

characteristic: environmental medium

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Clostridia bacterium

characteristic: organism

characteristic: project name

text: uncultured Clostridia bacterium

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR15730548_bin.13_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: feces

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina HiSeq 4000

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR15730548_bin.26_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: not provided

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

text: CheckM

text: feces

characteristic: environmental medium

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Roseburia sp.

characteristic: organism

characteristic: project name

text: uncultured Roseburia sp.

text: Illumina HiSeq 4000

text: multi-marker approach

text: Metagenome-assembled genome: SRR15730548_bin.39_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: feces

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Lachnospiraceae bacterium

text: We performed a deep whole metagenomic shotgun sequencing on mouse samples. Metagenomic de-novo assembly was performed on the samples.

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR15730548_bin.74_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: not provided

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR15730549 of study SRP335854.

text: feces

characteristic: environmental medium

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Clostridia bacterium

characteristic: organism

characteristic: project name

text: uncultured Clostridia bacterium

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Ruminococcaceae;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR15730549_bin.24_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: feces

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina HiSeq 4000

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR15730549_bin.60_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: not provided

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR15730550 of study SRP335854.

text: feces

characteristic: environmental medium

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Angelakisella sp.

characteristic: organism

characteristic: project name

text: uncultured Angelakisella sp.

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Ruminococcaceae;g__Angelakisella;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR15730550_bin.33_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: feces

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina HiSeq 4000

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR15730550_bin.62_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: not provided

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR15730551 of study SRP335854.

text: feces

characteristic: environmental medium

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Clostridia bacterium

characteristic: organism

characteristic: project name

text: uncultured Clostridia bacterium

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR15730551_bin.100_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: feces

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Lachnospiraceae bacterium

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: Illumina HiSeq 4000

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR15730551_bin.24_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: not provided

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

text: CheckM

text: feces

characteristic: environmental medium

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

text: Acutalibacter muris

characteristic: organism

text: We performed a deep whole metagenomic shotgun sequencing on mouse samples. Metagenomic de-novo assembly was performed on the samples.

characteristic: project name

text: Acutalibacter muris

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Ruminococcaceae;g__Acutalibacter;s__Acutalibacter muris

text: multi-marker approach

text: Metagenome-assembled genome: SRR15730551_bin.37_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: feces

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

characteristic: metagenomic source

text: Emergencia timonensis

characteristic: sample derived from

text: Emergencia timonensis

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Clostridiales Family XIII. Incertae Sedis;g__Emergencia;s__Emergencia timonensis

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR15730551_bin.93_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: not provided

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR15730552 of study SRP335854.

text: feces

characteristic: environmental medium

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Eubacteriaceae bacterium

characteristic: organism

characteristic: project name

text: uncultured Eubacteriaceae bacterium

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Eubacteriaceae;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR15730552_bin.19_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: feces

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina HiSeq 4000

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR15730552_bin.40_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: not provided

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

text: CheckM

text: feces

characteristic: environmental medium

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

characteristic: project name

text: uncultured Lachnospiraceae bacterium

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR15730552_bin.93_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR15730553 of study SRP335854.

characteristic: description

text: feces

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Tenericutes bacterium

characteristic: sample derived from

text: uncultured Tenericutes bacterium

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Tenericutes;c__;o__;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR15730553_bin.107_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: not provided

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

text: CheckM

text: feces

characteristic: environmental medium

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Clostridia bacterium

characteristic: organism

characteristic: project name

text: uncultured Clostridia bacterium

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR15730553_bin.13_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: feces

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured bacterium

text: We performed a deep whole metagenomic shotgun sequencing on mouse samples. Metagenomic de-novo assembly was performed on the samples.

characteristic: sample derived from

text: uncultured bacterium

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__;c__;o__;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR15730553_bin.22_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: not provided

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

text: CheckM

text: feces

characteristic: environmental medium

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Clostridia bacterium

characteristic: organism

characteristic: project name

text: uncultured Clostridia bacterium

text: Illumina HiSeq 4000

text: multi-marker approach

text: Metagenome-assembled genome: SRR15730553_bin.3_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: feces

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Clostridium sp.

characteristic: sample derived from

text: uncultured Clostridium sp.

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Clostridium;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR15730553_bin.32_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: not provided

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

text: CheckM

text: feces

characteristic: environmental medium

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Clostridia bacterium

characteristic: organism

characteristic: project name

text: uncultured Clostridia bacterium

text: Illumina HiSeq 4000

text: multi-marker approach

text: Metagenome-assembled genome: SRR15730553_bin.39_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: feces

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Ruminococcaceae;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR15730553_bin.60_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: not provided

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

text: CheckM

text: feces

characteristic: environmental medium

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Emergencia sp.

characteristic: organism

characteristic: project name

text: uncultured Emergencia sp.

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Clostridiales Family XIII. Incertae Sedis;g__Emergencia;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR15730553_bin.86_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR15730554 of study SRP335854.

characteristic: description

text: feces

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Catabacteriaceae;g__Catabacter;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR15730554_bin.17_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: not provided

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

text: CheckM

text: feces

characteristic: environmental medium

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

text: Adlercreutzia equolifaciens

characteristic: organism

characteristic: project name

text: Adlercreutzia equolifaciens

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Actinobacteria;c__Coriobacteriia;o__Eggerthellales;f__Eggerthellaceae;g__Adlercreutzia;s__Adlercreutzia equolifaciens

text: multi-marker approach

text: Metagenome-assembled genome: SRR15730554_bin.27_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: feces

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Roseburia sp.

text: We performed a deep whole metagenomic shotgun sequencing on mouse samples. Metagenomic de-novo assembly was performed on the samples.

characteristic: sample derived from

text: uncultured Roseburia sp.

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Roseburia;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR15730554_bin.42_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: not provided

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

text: CheckM

text: feces

characteristic: environmental medium

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Clostridia bacterium

characteristic: organism

characteristic: project name

text: uncultured Clostridia bacterium

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR15730554_bin.61_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: feces

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina HiSeq 4000

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR15730554_bin.7_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: not provided

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR15730555 of study SRP335854.

text: feces

characteristic: environmental medium

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

characteristic: project name

text: uncultured Lachnospiraceae bacterium

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR15730555_bin.29_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: feces

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Firmicutes bacterium

characteristic: sample derived from

text: uncultured Firmicutes bacterium

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__;o__;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR15730555_bin.36_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: not provided

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR15730556 of study SRP335854.

text: feces

characteristic: environmental medium

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured bacterium

characteristic: organism

characteristic: project name

text: uncultured bacterium

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__;c__;o__;f__;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR15730556_bin.62_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR15730557 of study SRP335854.

characteristic: description

text: feces

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured bacterium

characteristic: sample derived from

text: uncultured bacterium

text: Illumina HiSeq 4000

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR15730557_bin.32_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: not provided

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR15730558 of study SRP335854.

text: feces

characteristic: environmental medium

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Clostridia bacterium

characteristic: organism

text: We performed a deep whole metagenomic shotgun sequencing on mouse samples. Metagenomic de-novo assembly was performed on the samples.

characteristic: project name

text: uncultured Clostridia bacterium

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Ruminococcaceae;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR15730558_bin.31_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: feces

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Hungatella sp.

characteristic: sample derived from

text: uncultured Hungatella sp.

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Hungatella;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR15730558_bin.49_metawrap_v1.3_MAG


E-MTAB-12466:Sample 16

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Hebrew University of Jerusalem

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12466:Sample 16

tag: Namespace:The Hebrew University of Jerusalem

characteristic: Submitter Id

text: ArrayExpress

text: Protocols: As a model sRNA, we use the well-known sRNA GcvB, a sRNA that regulates genes involved in amino acid metabolism and transport. To study the cooperation between GcvB and RNase E we apply the mutant cycle approach to four strains of E. coli: wt; GcvB mutant/RNase E wt; GcvB wt/RNase E mutant; GcvB mutant/RNase E mutant. Bacteria were centrifuged for 5 min at 4°C,4500g, resuspended in 50 µl TE (10 mM Tris-HCl pH 7.5, 1 mM EDTA) and mixed with 5 ul of 9 mg/ml lysozyme prior to freezing the sample at liquid nitrogen and storing it at -80°C. Single colonies were grown in LB-Amp at 30°C while shaking over night. The next day, each culture was diluted 1:100 into two flasks containing fresh LB-Amp and grown at 30°C to an OD600 of 0.3. Then, one flask was transferred to 44°C while the other one was left at 30°C. After 20 min both cultures were treated with IPTG at a final concentration of 1 mM, and left for an additional 10 min at the respective temperatures. Cultures were collected. Frozen samples were subjected to two cycles of thawing at 37°C and refreezing in liquid nitrogen. Next, the samples were re-suspended thoroughly to homogenization with 1 mL TriReagent (pre-warmed to room temperature) and incubated for 5 min at room temperature. Two hundred microliters of chloroform were added, and the tubes content was mixed by inversing the tubes for 15 s. The samples were incubated for 10 min at room temperature, centrifuged (17,000g, 10 min at 4C) and the upper phase was collected and transferred into new Eppendorf tubes. For RNA precipitation, 500 l isopropanol were added, the tube contents were mixed thoroughly by inversion of the tubes and incubated for 10 min at room temperature. The tubes were centrifuged (17,000g,15 min at 4°C) and the supernatant was discarded. The pellets were washed twice by addition of 1 mL of freshly made 75% (vol/vol) ethanol, followed by centrifugation (17,000g, 5 min at 4°C) and removal of the supernatant. Pellets were air dried by at room temperature, and then re-suspended in 300 L nuclease free water and stored at -20°C. The RNA concentration was measured using Nanodrop (ThermoFisher Scientific). tagRNA-seq analysis of the transcriptional organization of a bacterium by vertis Biotechnology AG- tagRNA-seq is a modified RNA-seq method which is based on the differential labelling of 5' RNA ends. It enables to discriminate primary from processed 5' RNA ends. The method is described in detail by Innocenti, N. et al. 2015. RNA (New York, N.Y.). 21, 5 (May 2015), 1018-30 It is based on specific ligation of distinct sequence tags to processed start sites (PSS) and transcription start sites (TSS), thereby gaining insight into regulatory processes at transcriptional and post-transcriptional levels.

text: wild type genotype

characteristic: genotype

text: LB-Amp broth

characteristic: growth condition

text: Escherichia coli str. K-12 substr. MG1655

characteristic: organism

text: whole organism

characteristic: organism part

text: RNA-seq

characteristic: protocol

characteristic: strain

unit: Celsius

characteristic: temperature

text: Sample 16

characteristic: title


E-MTAB-12466:Sample 17

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: The Hebrew University of Jerusalem

text: public

text: E-MTAB-12466:Sample 17

text: ArrayExpress

characteristic: broker name

characteristic: description

text: wild type genotype

text: LB-Amp broth

text: whole organism

text: RNA-seq

text: Sample 17


E-MTAB-12466:Sample 18

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Hebrew University of Jerusalem

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12466:Sample 18

text: ArrayExpress

text: wild type genotype

text: LB-Amp broth

text: whole organism

text: RNA-seq

text: Sample 18


E-MTAB-12466:Sample 4

text: The Hebrew University of Jerusalem

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12466:Sample 4

characteristic: Submitter Id

text: ArrayExpress

text: wild type genotype

text: LB-Amp broth

text: whole organism

text: RNA-seq

text: Sample 4


E-MTAB-12466:Sample 5

text: The Hebrew University of Jerusalem

text: public

text: E-MTAB-12466:Sample 5

text: ArrayExpress

text: wild type genotype

characteristic: genotype

text: LB-Amp broth

characteristic: growth condition

characteristic: organism

text: whole organism

characteristic: organism part

text: RNA-seq

text: Sample 5


E-MTAB-12466:Sample 6

text: The Hebrew University of Jerusalem

text: public

text: E-MTAB-12466:Sample 6

text: ArrayExpress

text: wild type genotype

text: LB-Amp broth

text: whole organism

text: RNA-seq

characteristic: protocol

characteristic: strain

characteristic: temperature

text: Sample 6

characteristic: title


E-MTAB-12466:Sample 13

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: The Hebrew University of Jerusalem

text: public

text: gcvB knockdown

characteristic: RNA interference

text: E-MTAB-12466:Sample 13

text: ArrayExpress

characteristic: broker name

characteristic: description

text: wild type genotype

text: LB-Amp broth

text: whole organism

text: RNA-seq

text: Sample 13


E-MTAB-12466:Sample 14

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Hebrew University of Jerusalem

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: gcvB knockdown

text: E-MTAB-12466:Sample 14

text: ArrayExpress

text: wild type genotype

text: LB-Amp broth

text: whole organism

text: RNA-seq

text: Sample 14


E-MTAB-12466:Sample 15

text: The Hebrew University of Jerusalem

text: public

characteristic: INSDC status

text: gcvB knockdown

characteristic: SRA accession

text: E-MTAB-12466:Sample 15

characteristic: Submitter Id

text: ArrayExpress

text: wild type genotype

characteristic: genotype

text: LB-Amp broth

characteristic: growth condition

text: whole organism

text: RNA-seq

text: Sample 15


E-MTAB-12466:Sample 31

text: The Hebrew University of Jerusalem

text: public

text: gcvB knockdown

text: E-MTAB-12466:Sample 31

text: ArrayExpress

text: Protocols: As a model sRNA, we use the well-known sRNA GcvB, a sRNA that regulates genes involved in amino acid metabolism and transport. To study the cooperation between GcvB and RNase E we apply the mutant cycle approach to four strains of E. coli: wt; GcvB mutant/RNase E wt; GcvB wt/RNase E mutant; GcvB mutant/RNase E mutant. Bacteria were centrifuged for 5 min at 4°C,4500g, resuspended in 50 µl TE (10 mM Tris-HCl pH 7.5, 1 mM EDTA) and mixed with 5 ul of 9 mg/ml lysozyme prior to freezing the sample at liquid nitrogen and storing it at -80°C. Single colonies were grown in LB-Amp at 30°C while shaking over night. The next day, each culture was diluted 1:100 into two flasks containing fresh LB-Amp and grown at 30°C to an OD600 of 0.3. Then, one flask was transferred to 44°C while the other one was left at 30°C. After 20 min both cultures were treated with IPTG at a final concentration of 1 mM, and left for an additional 10 min at the respective temperatures. Cultures were collected. Frozen samples were subjected to two cycles of thawing at 37°C and refreezing in liquid nitrogen. Next, the samples were re-suspended thoroughly to homogenization with 1 mL TriReagent (pre-warmed to room temperature) and incubated for 5 min at room temperature. Two hundred microliters of chloroform were added, and the tubes content was mixed by inversing the tubes for 15 s. The samples were incubated for 10 min at room temperature, centrifuged (17,000g, 10 min at 4C) and the upper phase was collected and transferred into new Eppendorf tubes. For RNA precipitation, 500 l isopropanol were added, the tube contents were mixed thoroughly by inversion of the tubes and incubated for 10 min at room temperature. The tubes were centrifuged (17,000g,15 min at 4°C) and the supernatant was discarded. The pellets were washed twice by addition of 1 mL of freshly made 75% (vol/vol) ethanol, followed by centrifugation (17,000g, 5 min at 4°C) and removal of the supernatant. Pellets were air dried by at room temperature, and then re-suspended in 300 L nuclease free water and stored at -20°C. The RNA concentration was measured using Nanodrop (ThermoFisher Scientific). TIER-seq by Vertis Biotechnologie AG - enables the sequencing of 5′-ends of bacterial transcripts through the adaptor ligation site to the RNA 5′-starts.

text: wild type genotype

text: LB-Amp broth

characteristic: organism

text: whole organism

characteristic: organism part

text: TIER-seq

characteristic: protocol

characteristic: strain

characteristic: temperature

text: Sample 31


E-MTAB-12466:Sample 32

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: The Hebrew University of Jerusalem

text: public

text: gcvB knockdown

characteristic: RNA interference

text: E-MTAB-12466:Sample 32

text: ArrayExpress

characteristic: broker name

text: wild type genotype

text: LB-Amp broth

text: whole organism

text: TIER-seq

text: Sample 32

characteristic: title


E-MTAB-12466:Sample 33

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Hebrew University of Jerusalem

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: gcvB knockdown

text: E-MTAB-12466:Sample 33

text: ArrayExpress

characteristic: description

text: wild type genotype

text: LB-Amp broth

text: whole organism

text: TIER-seq

text: Sample 33


E-MTAB-12466:Sample 1

text: The Hebrew University of Jerusalem

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: gcvB knockdown

characteristic: SRA accession

text: E-MTAB-12466:Sample 1

characteristic: Submitter Id

text: ArrayExpress

text: wild type genotype

characteristic: genotype

text: LB-Amp broth

text: whole organism

text: RNA-seq

text: Sample 1


E-MTAB-12466:Sample 2

text: The Hebrew University of Jerusalem

text: public

text: gcvB knockdown

text: E-MTAB-12466:Sample 2

text: ArrayExpress

text: wild type genotype

text: LB-Amp broth

characteristic: growth condition

characteristic: organism

text: whole organism

characteristic: organism part

text: RNA-seq

characteristic: protocol

characteristic: strain

text: Sample 2


E-MTAB-12466:Sample 25

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: The Hebrew University of Jerusalem

text: public

text: gcvB knockdown

characteristic: RNA interference

text: E-MTAB-12466:Sample 25

text: ArrayExpress

characteristic: broker name

text: wild type genotype

text: LB-Amp broth

text: whole organism

text: TIER-seq

characteristic: temperature

text: Sample 25


E-MTAB-12466:Sample 26

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Hebrew University of Jerusalem

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: gcvB knockdown

text: E-MTAB-12466:Sample 26

text: ArrayExpress

text: wild type genotype

text: LB-Amp broth

text: whole organism

text: TIER-seq

text: Sample 26

characteristic: title


E-MTAB-12466:Sample 27

text: The Hebrew University of Jerusalem

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: gcvB knockdown

characteristic: SRA accession

text: E-MTAB-12466:Sample 27

characteristic: Submitter Id

text: ArrayExpress

characteristic: description

text: wild type genotype

text: LB-Amp broth

text: whole organism

text: TIER-seq

text: Sample 27


E-MTAB-12466:Sample 3

text: The Hebrew University of Jerusalem

text: public

text: gcvB knockdown

text: E-MTAB-12466:Sample 3

text: ArrayExpress

text: wild type genotype

characteristic: genotype

text: LB-Amp broth

characteristic: growth condition

characteristic: organism

text: whole organism

characteristic: organism part

text: RNA-seq

characteristic: protocol

text: Sample 3


E-MTAB-12466:Sample 8

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: The Hebrew University of Jerusalem

text: public

text: gcvB knockdown

characteristic: RNA interference

text: E-MTAB-12466:Sample 8

text: ArrayExpress

characteristic: broker name

text: RNAseE 3071 loss of function mutation

text: LB-Amp broth

text: whole organism

text: RNA-seq

characteristic: strain

text: Sample 8


E-MTAB-12466:Sample 22

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Hebrew University of Jerusalem

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12466:Sample 22

text: ArrayExpress

text: RNAseE 3071 loss of function mutation

text: LB-Amp broth

text: whole organism

text: RNA-seq

characteristic: temperature

text: Sample 22


E-MTAB-12466:Sample 23

text: The Hebrew University of Jerusalem

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12466:Sample 23

characteristic: Submitter Id

text: ArrayExpress

text: RNAseE 3071 loss of function mutation

text: LB-Amp broth

text: whole organism

text: RNA-seq

text: Sample 23

characteristic: title


E-MTAB-12466:Sample 24

text: The Hebrew University of Jerusalem

text: public

text: E-MTAB-12466:Sample 24

text: ArrayExpress

characteristic: description

text: RNAseE 3071 loss of function mutation

characteristic: genotype

text: LB-Amp broth

characteristic: growth condition

characteristic: organism

text: whole organism

characteristic: organism part

text: RNA-seq

text: Sample 24


E-MTAB-12466:Sample 10

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: The Hebrew University of Jerusalem

text: public

text: E-MTAB-12466:Sample 10

text: ArrayExpress

characteristic: broker name

text: RNAseE 3071 loss of function mutation

text: LB-Amp broth

text: whole organism

text: RNA-seq

characteristic: protocol

text: Sample 10


E-MTAB-12466:Sample 11

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Hebrew University of Jerusalem

characteristic: INSDC center name

text: public

text: E-MTAB-12466:Sample 11

text: ArrayExpress

text: RNAseE 3071 loss of function mutation

text: LB-Amp broth

text: whole organism

text: RNA-seq

characteristic: strain

text: Sample 11


E-MTAB-12466:Sample 12

text: The Hebrew University of Jerusalem

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12466:Sample 12

text: ArrayExpress

text: RNAseE 3071 loss of function mutation

text: LB-Amp broth

text: whole organism

text: RNA-seq

characteristic: temperature

text: Sample 12


E-MTAB-12466:Sample 19

text: The Hebrew University of Jerusalem

text: public

text: gcvB knockdown

characteristic: RNA interference

characteristic: SRA accession

text: E-MTAB-12466:Sample 19

characteristic: Submitter Id

text: ArrayExpress

characteristic: description

text: RNAseE 3071 loss of function mutation

text: LB-Amp broth

text: whole organism

text: RNA-seq

text: Sample 19

characteristic: title


E-MTAB-12466:Sample 20

characteristic: ENA-CHECKLIST

text: The Hebrew University of Jerusalem

text: public

text: gcvB knockdown

text: E-MTAB-12466:Sample 20

text: ArrayExpress

text: RNAseE 3071 loss of function mutation

characteristic: genotype

text: LB-Amp broth

characteristic: growth condition

characteristic: organism

text: whole organism

characteristic: organism part

text: RNA-seq

text: Sample 20


E-MTAB-12466:Sample 21

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Hebrew University of Jerusalem

text: public

text: gcvB knockdown

text: E-MTAB-12466:Sample 21

text: ArrayExpress

characteristic: broker name

text: RNAseE 3071 loss of function mutation

text: LB-Amp broth

text: whole organism

text: RNA-seq

characteristic: protocol

text: Sample 21


E-MTAB-12466:Sample 34

text: The Hebrew University of Jerusalem

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: gcvB knockdown

text: E-MTAB-12466:Sample 34

text: ArrayExpress

text: RNAseE 3071 loss of function mutation

text: LB-Amp broth

text: whole organism

text: TIER-seq

characteristic: strain

text: Sample 34


E-MTAB-12466:Sample 35

text: The Hebrew University of Jerusalem

text: public

text: gcvB knockdown

characteristic: RNA interference

characteristic: SRA accession

text: E-MTAB-12466:Sample 35

characteristic: Submitter Id

text: ArrayExpress

characteristic: description

text: RNAseE 3071 loss of function mutation

text: LB-Amp broth

text: whole organism

text: TIER-seq

characteristic: temperature

text: Sample 35


E-MTAB-12466:Sample 36

characteristic: ENA-CHECKLIST

text: The Hebrew University of Jerusalem

text: public

text: gcvB knockdown

text: E-MTAB-12466:Sample 36

text: ArrayExpress

text: RNAseE 3071 loss of function mutation

characteristic: genotype

text: LB-Amp broth

characteristic: growth condition

characteristic: organism

text: whole organism

text: TIER-seq

text: Sample 36

characteristic: title


E-MTAB-12466:Sample 28

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Hebrew University of Jerusalem

text: public

text: gcvB knockdown

text: E-MTAB-12466:Sample 28

text: ArrayExpress

characteristic: broker name

text: RNAseE 3071 loss of function mutation

text: LB-Amp broth

text: whole organism

characteristic: organism part

text: TIER-seq

text: Sample 28


E-MTAB-12466:Sample 29

text: The Hebrew University of Jerusalem

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: gcvB knockdown

text: E-MTAB-12466:Sample 29

text: ArrayExpress

text: RNAseE 3071 loss of function mutation

text: LB-Amp broth

text: whole organism

text: TIER-seq

characteristic: protocol

text: Sample 29


E-MTAB-12466:Sample 30

text: The Hebrew University of Jerusalem

text: public

text: gcvB knockdown

characteristic: RNA interference

characteristic: SRA accession

text: E-MTAB-12466:Sample 30

characteristic: Submitter Id

text: ArrayExpress

characteristic: description

text: RNAseE 3071 loss of function mutation

text: LB-Amp broth

text: whole organism

text: TIER-seq

characteristic: strain

text: Sample 30


E-MTAB-12466:Sample 7

characteristic: ENA-CHECKLIST

text: The Hebrew University of Jerusalem

text: public

text: gcvB knockdown

text: E-MTAB-12466:Sample 7

text: ArrayExpress

text: RNAseE 3071 loss of function mutation

characteristic: genotype

text: LB-Amp broth

characteristic: growth condition

characteristic: organism

text: whole organism

text: RNA-seq

characteristic: temperature

text: Sample 7


E-MTAB-12466:Sample 9

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Hebrew University of Jerusalem

text: public

text: gcvB knockdown

text: E-MTAB-12466:Sample 9

text: ArrayExpress

characteristic: broker name

text: RNAseE 3071 loss of function mutation

text: LB-Amp broth

text: whole organism

text: RNA-seq

text: Sample 9

characteristic: title


text: Humboldt Universitaet zu Berlin

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-14-12-2022-17:14:53:542-31140

tag: Namespace:Humboldt Universitaet zu Berlin

text: Humboldt-Universit�t zu Berlin / Phytomedicine

characteristic: collecting institution

characteristic: collection date

text: Carmen B�ttner

characteristic: collector name

text: mixed leaf sample

text: Germany

characteristic: geographic location (country and/or sea)

text: Brandenburg

characteristic: geographic location (region and locality)

text: small-leaved lime

characteristic: host common name

text: not provided

characteristic: host health state

text: Tilia cordata

characteristic: host scientific name

text: hermaphrodite

characteristic: host sex

characteristic: host subject id

characteristic: isolate

text: leaves

characteristic: isolation source host-associated

text: Cytorhabdovirus tiliae

text: Cytorhabdovirus tiliae

characteristic: virus identifier


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Dundee

characteristic: INSDC center name

text: public

characteristic: SRA accession

tag: Namespace:University of Dundee

characteristic: Submitter Id

characteristic: description

text: Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10

characteristic: organism

text: DK1042_NCIB 3610 comIQ12L

characteristic: title


text: Shanghai Tenth People's Hospital

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

tag: Namespace:Shanghai Tenth People's Hospital

text: ArrayExpress

characteristic: broker name

text: Protocols: Strains were maintained at -80˚C. Strains were streaked on YPD plates. The plates were incubated at 37˚C overnight. Then several colonies were picked up and suspended in DDW. Cell density was adjusted to 10,000 cells/ml, then 100µl of cell suspension was plated on YPD plates. The plates were incubated at 37˚C overnight. Colonies were picked up and suspended in DDW, then cell pellets were obtained by centrifuging at highest speed for 1min. We used the phenol-chloroform method to extract Genomic DNA. Cell pellets were suspended in 200µl of lysis buffer, add 100µl of acid washed glass beads, and 0,2ml phenol:chloroform:isoamyl alcohol (25:24:1). Vortex for 30min at 4˚C. Add 200µl TE pH7.5. Centrifuge for 5min at maximum speed. Transfer aqueous layer to a new 1.5ml EP tube. Add 1ml 100% ethanol. Precipitate 1h at -20˚C. Pellet DNA by centrifuging for 10min at maximum speed. Discard the supernatant. Add 400µl TE pH 7.5. Add 3ul of RNase A (10mg/ml). Incubate at 37˚C for 30min. Add 10µl of 3M NaOAc and 1ml of 100% ethanol. Mix by inversion. Precipitate 30min at -20˚C. Pellet DNA by centrifuging for 10min at maximum speed. Discard supernatant. Air-dry the pellet overnight. Resuspend in 50µl water. 1µg Genomic DNA was randomly fragmented by Covaris, followed by fragments selection by Agencourt AMPure XP-Medium kit to an average size of 200~400 bp. Selected fragments were end repaired and 3\adenylate, then the adaptors were ligated to the end of these 3\adenylate fragments. The products were amplified by PCR and purified by the Agencourt AMPure XP-Medium kit. The purified double stranded PCR products were heat denatured to single strand, then circularised by the splint oligo sequence. The single strand circle DNA (ssCirDNA) were formatted as the final library and qualified by QC.

text: wild type genotype

characteristic: genotype

text: Candida albicans

text: Candida albicans

text: Candida albicans


text: Shanghai Tenth People's Hospital

text: public

text: ArrayExpress

text: ANEUPLOID

text: Candida albicans

text: Candida albicans

text: Candida albicans


text: public

tag: Namespace:Omics Core Facility NGS Unit Medical School OWL & CeBiTec Bielefeld University

text: ArrayExpress

text: Protocols: Here, we explored membrane adaptations of Pseudomonas putida KT2440, in particular outer membrane vesicle (OMV) formation in response to 1-octanol, PQS and prodigiosin, causing chemical membrane stress via RNA-seq of mRNA. Pseudomonas putida wild type KT2440 (Nelson et al. 2002) and the derived strains P. putida pig21 were cultivated in biological triplicates under continuous shaking (130 rpm) at 30 °C in 10 mL LB (lysogeny broth) medium (10 g L-1 tryptone, 5 g L-1 yeast extract, 10 g L-1 sodium chloride; Carl Roth®, Karlsruhe, Germany). Antibiotics were added to the culture medium when appropriate to the following final concentrations: 25 µg mL-1 kanamycin, 25 µg mL-1 irgasan, 25 µg mL-1 gentamicin, 50 µg mL-1 tetracycline. For chemical induction of OMV formation, P. putida KT2440 was exposed to 1 mM 1-octanol or 50 µM PQS (Pseudomonas quinolone signal) after reaching the logarithmic growth phase. Cell pellet was harvested after 7 h, adjusted to an optical density (OD700 nm) of 1 by centirfugation and flash frozen in liquid nitrogen. Total RNA was isolated from 3 biological replicates using Quick-RNA Miniprep Plus kit (Zymo Research). The samples were treated with DNase (Zymo Research) and RNA was again purified with an RNA Clean&Concentrator-5 kit (Zymo Research). Ribosomal rRNA was removed with a riboPOOL for bacteria (siTOOLs Biotech GmbH). The purity of RNA and removal of rRNA was then tested with an Agilent RNA Pico 6000 kit and an Agilent 2100 Bioanalyzer (Agilent Technologies). After ribosomal rRNA was removed with a riboPOOL for bacteria (siTOOLs Biotech GmbH), Illumina TruSeq Stranded mRNA Sample Preparation guide was then used to construct the cDNA library.

text: wild type genotype

text: LB broth

characteristic: growth condition

text: Pseudomonas putida KT2440

text: Pseudomonas putida KT2440

text: whole organism

characteristic: organism part

text: Pseudomonas putida KT2440

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Omics Core Facility NGS Unit Medical School OWL & CeBiTec Bielefeld University

text: public

text: ArrayExpress

text: wild type genotype

text: LB broth

text: Pseudomonas putida KT2440

text: Pseudomonas putida KT2440

text: whole organism

text: Pseudomonas putida KT2440


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR15510217 of study SRP333077.

text: feces

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Firmicutes bacterium

text: Intestinal Virome Diversity Correlates with Intestinal Microbiome Diversity in Ob/Ob and Apoe-/- Mice

characteristic: project name

characteristic: sample derived from

text: uncultured Firmicutes bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__;o__;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR15510217_bin.50_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR15510223 of study SRP333077.

characteristic: description

text: feces

text: China

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured bacterium

characteristic: organism

text: uncultured bacterium

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__;c__;o__;f__;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR15510223_bin.42_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Bacillales bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Bacillales bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR15510223_bin.53_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR15510224 of study SRP333077.

characteristic: description

text: feces

text: China

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Hungatella sp.

characteristic: organism

text: uncultured Hungatella sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Hungatella;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR15510224_bin.34_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR15510225 of study SRP333077.

text: feces

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Enterorhabdus sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Enterorhabdus sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Actinobacteria;c__Coriobacteriia;o__Eggerthellales;f__Eggerthellaceae;g__Enterorhabdus;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR15510225_bin.45_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR15510227 of study SRP333077.

characteristic: description

text: feces

text: China

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Alistipes sp.

characteristic: organism

text: uncultured Alistipes sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Rikenellaceae;g__Alistipes;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR15510227_bin.21_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR15510228 of study SRP333077.

text: feces

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Prevotella sp.

text: Intestinal Virome Diversity Correlates with Intestinal Microbiome Diversity in Ob/Ob and Apoe-/- Mice

characteristic: project name

characteristic: sample derived from

text: uncultured Prevotella sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Prevotellaceae;g__Prevotella;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR15510228_bin.14_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR15510229 of study SRP333077.

characteristic: description

text: feces

text: China

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Clostridia bacterium

characteristic: organism

text: uncultured Clostridia bacterium

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR15510229_bin.12_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR15510232 of study SRP333077.

text: feces

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Tenericutes bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Tenericutes bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Tenericutes;c__;o__;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR15510232_bin.23_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR15510236 of study SRP333077.

characteristic: description

text: feces

text: China

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Porphyromonadaceae bacterium

characteristic: organism

text: uncultured Porphyromonadaceae bacterium

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Porphyromonadaceae;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR15510236_bin.33_metawrap_v1.3_MAG

characteristic: title