characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

characteristic: description

text: embryo

text: normal

text: L40764_Track-80115: 153673; L40764_Track-79540: 304283

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


status: PUBLIC

tag: attribute

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

tag: Namespace:The Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: Protocols: hESC-derived pancreatic cells at Stage 4 Day 1 of the pancreatic differentiation protocol (Rezania et al., 2014; Petersen et al., 2017) were dissociated into single cells with TrypLE and resuspended into a FACS buffer containing 0.5% BSA and 2 mM EDTA (Thermo Fisher) in 1X PBS. The cells were index-sorted according to their GFP expression into 384-well plates on a BD FACSAria and snap-frozen. Cells are processed with SMARTseq-2 protocol miniaturized and modified based on Picelli et al. 2013 (doi: 10.1038/nmeth.2639). In short, cells are sorted in 0.5 µl 0.2% Triton X-100 solution with RNase Inhibitor into a 384-well plate, frozen at -80°C. After thawing, the cell lysate is mixed with 0.5 ul of a primer mix (containing 5 mM dNTP (Invitrogen), 0.5 uM dT-primer*, 1 U RNase Inhibitor (NEB)). RNA is denatured (3 minutes 72 °C), followed by reverse transcription (42 °C 90 min + 70°C 15 min) after adding 1.5 ul RT buffer mix (1x superscript II buffer (Invitrogen), 1 M betaine, 5 mM DTT, 6 mM MgCl2, 1 uM TSO-primer*, 2.3 U RNase Inhibitor and 25 U Superscript II). The cDNA is amplified using 1x Kapa HiFi HotStart Readymix (Roche) and 0.1 uM UP-primer under following cycling conditions: 98°C for 3 min, 23 cycles [98°C 20 sec, 67°C 15 sec, 72°C 6 min] and final elongation at 72°C for 5 min. The amplified cDNA is purified using 1x volume of Sera-Mag SpeedBeads (GE Healthcare) and eluted in 12 ul nuclease free water. For library preparation 1.5 µl cDNA is desiccated and rehydrated in 1 ul Tagmentation mix (1x TTBL buffer, 0.1 ul Tagment DNA Enzyme; from TruePrep DNA Library Prep Kit V2 for Illumina; Vazyme) and tagmented (55°C, 5 min), followed by an index PCR with 1x concentrated KAPA Hifi HotStart Ready Mix and 0.7 µM dual indexing primers (72°C 3 min, 98°C 30 sec, 13 cycles [98°C 10 sec, 63°C 20 sec, 72°C 1 min], 72°C 5 min). After PCR, libraries are quantified with AccuBlue Broad range chemistry (Biotium), equimolarly pooled and purified twice with 0.9x volume Sera-Mag SpeedBeads.

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40724_Track-80102: 233318; L40724_Track-79490: 465318

characteristic: number of reads

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP positive

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: The Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

characteristic: description

text: embryo

text: normal

text: L40782_Track-79994: 163997; L40782_Track-79317: 319477

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40815_Track-80260: 152179; L40815_Track-79637: 367647

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40749_Track-80215: 46773; L40749_Track-79601: 114245

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP positive

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40757_Track-80156: 210660; L40757_Track-79330: 422443

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40794_Track-80160: 229543; L40794_Track-79512: 452093

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

text: normal

text: L40786_Track-80208: 60342; L40786_Track-79597: 130492

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP positive

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

characteristic: description

text: embryo

text: normal

text: L40744_Track-80187: 198771; L40744_Track-79281: 389554

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40743_Track-79970: 227565; L40743_Track-79443: 456695

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40767_Track-80067: 267386; L40767_Track-79282: 537748

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP positive

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40780_Track-80276: 19866; L40780_Track-79627: 41600

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40761_Track-79949: 161294; L40761_Track-79487: 321940

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

text: normal

text: L40760_Track-80219: 24882; L40760_Track-79585: 48257

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP positive

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

characteristic: description

text: embryo

text: normal

text: L40726_Track-79917: 126871; L40726_Track-79544: 248175

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40809_Track-80183: 177673; L40809_Track-79399: 355560

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: Protocols: hESC-derived pancreatic cells at Stage 4 Day 1 of the pancreatic differentiation protocol (Rezania et al., 2014; Petersen et al., 2017) were dissociated into single cells with TrypLE and resuspended into a FACS buffer containing 0.5% BSA and 2 mM EDTA (Thermo Fisher) in 1X PBS. The cells were index-sorted according to their GFP expression into 384-well plates on a BD FACSAria and snap-frozen. Cells are processed with SMARTseq-2 protocol miniaturized and modified based on Picelli et al. 2013 (doi: 10.1038/nmeth.2639). In short, cells are sorted in 0.5 µl 0.2% Triton X-100 solution with RNase Inhibitor into a 384-well plate, frozen at -80°C. After thawing, the cell lysate is mixed with 0.5 ul of a primer mix (containing 5 mM dNTP (Invitrogen), 0.5 uM dT-primer*, 1 U RNase Inhibitor (NEB)). RNA is denatured (3 minutes 72 °C), followed by reverse transcription (42 °C 90 min + 70°C 15 min) after adding 1.5 ul RT buffer mix (1x superscript II buffer (Invitrogen), 1 M betaine, 5 mM DTT, 6 mM MgCl2, 1 uM TSO-primer*, 2.3 U RNase Inhibitor and 25 U Superscript II). The cDNA is amplified using 1x Kapa HiFi HotStart Readymix (Roche) and 0.1 uM UP-primer under following cycling conditions: 98°C for 3 min, 23 cycles [98°C 20 sec, 67°C 15 sec, 72°C 6 min] and final elongation at 72°C for 5 min. The amplified cDNA is purified using 1x volume of Sera-Mag SpeedBeads (GE Healthcare) and eluted in 12 ul nuclease free water. For library preparation 1.5 µl cDNA is desiccated and rehydrated in 1 ul Tagmentation mix (1x TTBL buffer, 0.1 ul Tagment DNA Enzyme; from TruePrep DNA Library Prep Kit V2 for Illumina; Vazyme) and tagmented (55°C, 5 min), followed by an index PCR with 1x concentrated KAPA Hifi HotStart Ready Mix and 0.7 µM dual indexing primers (72°C 3 min, 98°C 30 sec, 13 cycles [98°C 10 sec, 63°C 20 sec, 72°C 1 min], 72°C 5 min). After PCR, libraries are quantified with AccuBlue Broad range chemistry (Biotium), equimolarly pooled and purified twice with 0.9x volume Sera-Mag SpeedBeads.

text: embryo

text: normal

text: L40763_Track-80069: 169930; L40763_Track-79291: 340766

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP positive

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: External Id

text: The Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40796_Track-80241: 123867; L40796_Track-79625: 299586

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40793_Track-80245: 214770; L40793_Track-79644: 515697

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

text: normal

text: L40748_Track-79646: 89768

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP positive

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

characteristic: description

text: embryo

text: normal

text: L40746_Track-80016: 243684; L40746_Track-79297: 479279

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40735_Track-80155: 137856; L40735_Track-79320: 276427

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40772_Track-79911: 170812; L40772_Track-79442: 313850

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP positive

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40770_Track-79966: 256751; L40770_Track-79389: 503451

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40750_Track-80013: 95595; L40750_Track-79537: 188421

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

text: normal

text: L40723_Track-80170: 195579; L40723_Track-79508: 391147

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP positive

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

characteristic: description

text: embryo

text: normal

text: L40820_Track-80176: 167085; L40820_Track-79504: 327144

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40728_Track-79648: 296801

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40814_Track-80197: 183457; L40814_Track-79305: 364028

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP positive

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40739_Track-80230: 85322; L40739_Track-79581: 207631

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40779_Track-79928: 145327; L40779_Track-79279: 284412

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

text: normal

text: L40801_Track-80150: 128059; L40801_Track-79419: 253894

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP positive

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

characteristic: description

text: embryo

text: normal

text: L40776_Track-79914: 176525; L40776_Track-79310: 348335

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: Protocols: hESC-derived pancreatic cells at Stage 4 Day 1 of the pancreatic differentiation protocol (Rezania et al., 2014; Petersen et al., 2017) were dissociated into single cells with TrypLE and resuspended into a FACS buffer containing 0.5% BSA and 2 mM EDTA (Thermo Fisher) in 1X PBS. The cells were index-sorted according to their GFP expression into 384-well plates on a BD FACSAria and snap-frozen. Cells are processed with SMARTseq-2 protocol miniaturized and modified based on Picelli et al. 2013 (doi: 10.1038/nmeth.2639). In short, cells are sorted in 0.5 µl 0.2% Triton X-100 solution with RNase Inhibitor into a 384-well plate, frozen at -80°C. After thawing, the cell lysate is mixed with 0.5 ul of a primer mix (containing 5 mM dNTP (Invitrogen), 0.5 uM dT-primer*, 1 U RNase Inhibitor (NEB)). RNA is denatured (3 minutes 72 °C), followed by reverse transcription (42 °C 90 min + 70°C 15 min) after adding 1.5 ul RT buffer mix (1x superscript II buffer (Invitrogen), 1 M betaine, 5 mM DTT, 6 mM MgCl2, 1 uM TSO-primer*, 2.3 U RNase Inhibitor and 25 U Superscript II). The cDNA is amplified using 1x Kapa HiFi HotStart Readymix (Roche) and 0.1 uM UP-primer under following cycling conditions: 98°C for 3 min, 23 cycles [98°C 20 sec, 67°C 15 sec, 72°C 6 min] and final elongation at 72°C for 5 min. The amplified cDNA is purified using 1x volume of Sera-Mag SpeedBeads (GE Healthcare) and eluted in 12 ul nuclease free water. For library preparation 1.5 µl cDNA is desiccated and rehydrated in 1 ul Tagmentation mix (1x TTBL buffer, 0.1 ul Tagment DNA Enzyme; from TruePrep DNA Library Prep Kit V2 for Illumina; Vazyme) and tagmented (55°C, 5 min), followed by an index PCR with 1x concentrated KAPA Hifi HotStart Ready Mix and 0.7 µM dual indexing primers (72°C 3 min, 98°C 30 sec, 13 cycles [98°C 10 sec, 63°C 20 sec, 72°C 1 min], 72°C 5 min). After PCR, libraries are quantified with AccuBlue Broad range chemistry (Biotium), equimolarly pooled and purified twice with 0.9x volume Sera-Mag SpeedBeads.

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40751_Track-80032: 130620; L40751_Track-79454: 260258

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: The Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40752_Track-79993: 130168; L40752_Track-79359: 254078

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP positive

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40771_Track-80054: 236645; L40771_Track-79316: 475540

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40795_Track-80006: 229227; L40795_Track-79362: 459828

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

text: normal

text: L40732_Track-79950: 21126; L40732_Track-79558: 33749

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP positive

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

characteristic: description

text: embryo

text: normal

text: L40800_Track-79946: 120213; L40800_Track-79513: 235366

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40811_Track-80007: 208372; L40811_Track-79551: 418957

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40769_Track-79972: 255992; L40769_Track-79554: 511034

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP positive

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40787_Track-80017: 167822; L40787_Track-79340: 337800

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40791_Track-80248: 118919; L40791_Track-79628: 288817

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

text: normal

text: L40730_Track-80200: 37376; L40730_Track-79334: 59389

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP positive

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

characteristic: description

text: embryo

text: normal

text: L40731_Track-80229: 5748; L40731_Track-79575: 12297

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40792_Track-80211: 98677; L40792_Track-79623: 232059

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40737_Track-79944: 118762; L40737_Track-79293: 236446

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP positive

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40755_Track-80138: 178116; L40755_Track-79557: 357752

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: Protocols: hESC-derived pancreatic cells at Stage 4 Day 1 of the pancreatic differentiation protocol (Rezania et al., 2014; Petersen et al., 2017) were dissociated into single cells with TrypLE and resuspended into a FACS buffer containing 0.5% BSA and 2 mM EDTA (Thermo Fisher) in 1X PBS. The cells were index-sorted according to their GFP expression into 384-well plates on a BD FACSAria and snap-frozen. Cells are processed with SMARTseq-2 protocol miniaturized and modified based on Picelli et al. 2013 (doi: 10.1038/nmeth.2639). In short, cells are sorted in 0.5 µl 0.2% Triton X-100 solution with RNase Inhibitor into a 384-well plate, frozen at -80°C. After thawing, the cell lysate is mixed with 0.5 ul of a primer mix (containing 5 mM dNTP (Invitrogen), 0.5 uM dT-primer*, 1 U RNase Inhibitor (NEB)). RNA is denatured (3 minutes 72 °C), followed by reverse transcription (42 °C 90 min + 70°C 15 min) after adding 1.5 ul RT buffer mix (1x superscript II buffer (Invitrogen), 1 M betaine, 5 mM DTT, 6 mM MgCl2, 1 uM TSO-primer*, 2.3 U RNase Inhibitor and 25 U Superscript II). The cDNA is amplified using 1x Kapa HiFi HotStart Readymix (Roche) and 0.1 uM UP-primer under following cycling conditions: 98°C for 3 min, 23 cycles [98°C 20 sec, 67°C 15 sec, 72°C 6 min] and final elongation at 72°C for 5 min. The amplified cDNA is purified using 1x volume of Sera-Mag SpeedBeads (GE Healthcare) and eluted in 12 ul nuclease free water. For library preparation 1.5 µl cDNA is desiccated and rehydrated in 1 ul Tagmentation mix (1x TTBL buffer, 0.1 ul Tagment DNA Enzyme; from TruePrep DNA Library Prep Kit V2 for Illumina; Vazyme) and tagmented (55°C, 5 min), followed by an index PCR with 1x concentrated KAPA Hifi HotStart Ready Mix and 0.7 µM dual indexing primers (72°C 3 min, 98°C 30 sec, 13 cycles [98°C 10 sec, 63°C 20 sec, 72°C 1 min], 72°C 5 min). After PCR, libraries are quantified with AccuBlue Broad range chemistry (Biotium), equimolarly pooled and purified twice with 0.9x volume Sera-Mag SpeedBeads.

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40824_Track-79675: 347309

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP negative

text: plate 0

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

text: normal

text: L40473_Track-80058: 143482; L40473_Track-79388: 285512

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP negative

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: The Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

characteristic: description

text: embryo

text: normal

text: L40529_Track-80025: 193497; L40529_Track-79373: 388117

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP negative

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40818_Track-80205: 212913; L40818_Track-79474: 419301

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40781_Track-79649: 155

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP positive

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40808_Track-80128: 147274; L40808_Track-79401: 289397

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40789_Track-79961: 198856; L40789_Track-79526: 400194

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

text: normal

text: L40835_Track-79669: 502628

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP positive

characteristic: phenotype

text: plate 0

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

characteristic: description

text: embryo

text: normal

text: L40816_Track-80099: 218869; L40816_Track-79541: 435887

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40740_Track-80065: 182031; L40740_Track-79407: 365508

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40729_Track-80038: 140597; L40729_Track-79444: 280789

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP positive

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40837_Track-79661: 794833

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 0

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40806_Track-80011: 262562; L40806_Track-79566: 519106

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

text: normal

text: L40790_Track-79926: 178269; L40790_Track-79336: 345039

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP positive

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

characteristic: description

text: embryo

text: normal

text: L40812_Track-80118: 188727; L40812_Track-79371: 369735

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40777_Track-80247: 141948; L40777_Track-79588: 342111

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40774_Track-80262: 186906; L40774_Track-79634: 396999

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP positive

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40784_Track-80246: 138866; L40784_Track-79610: 331245

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40768_Track-80111: 186769; L40768_Track-79496: 372121

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: Protocols: hESC-derived pancreatic cells at Stage 4 Day 1 of the pancreatic differentiation protocol (Rezania et al., 2014; Petersen et al., 2017) were dissociated into single cells with TrypLE and resuspended into a FACS buffer containing 0.5% BSA and 2 mM EDTA (Thermo Fisher) in 1X PBS. The cells were index-sorted according to their GFP expression into 384-well plates on a BD FACSAria and snap-frozen. Cells are processed with SMARTseq-2 protocol miniaturized and modified based on Picelli et al. 2013 (doi: 10.1038/nmeth.2639). In short, cells are sorted in 0.5 µl 0.2% Triton X-100 solution with RNase Inhibitor into a 384-well plate, frozen at -80°C. After thawing, the cell lysate is mixed with 0.5 ul of a primer mix (containing 5 mM dNTP (Invitrogen), 0.5 uM dT-primer*, 1 U RNase Inhibitor (NEB)). RNA is denatured (3 minutes 72 °C), followed by reverse transcription (42 °C 90 min + 70°C 15 min) after adding 1.5 ul RT buffer mix (1x superscript II buffer (Invitrogen), 1 M betaine, 5 mM DTT, 6 mM MgCl2, 1 uM TSO-primer*, 2.3 U RNase Inhibitor and 25 U Superscript II). The cDNA is amplified using 1x Kapa HiFi HotStart Readymix (Roche) and 0.1 uM UP-primer under following cycling conditions: 98°C for 3 min, 23 cycles [98°C 20 sec, 67°C 15 sec, 72°C 6 min] and final elongation at 72°C for 5 min. The amplified cDNA is purified using 1x volume of Sera-Mag SpeedBeads (GE Healthcare) and eluted in 12 ul nuclease free water. For library preparation 1.5 µl cDNA is desiccated and rehydrated in 1 ul Tagmentation mix (1x TTBL buffer, 0.1 ul Tagment DNA Enzyme; from TruePrep DNA Library Prep Kit V2 for Illumina; Vazyme) and tagmented (55°C, 5 min), followed by an index PCR with 1x concentrated KAPA Hifi HotStart Ready Mix and 0.7 µM dual indexing primers (72°C 3 min, 98°C 30 sec, 13 cycles [98°C 10 sec, 63°C 20 sec, 72°C 1 min], 72°C 5 min). After PCR, libraries are quantified with AccuBlue Broad range chemistry (Biotium), equimolarly pooled and purified twice with 0.9x volume Sera-Mag SpeedBeads.

text: embryo

text: normal

text: L40836_Track-79667: 485869

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP positive

characteristic: phenotype

text: plate 0

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

characteristic: description

text: embryo

text: normal

text: L40803_Track-80254: 75999; L40803_Track-79619: 185652

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: External Id

text: The Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40745_Track-79985: 287499; L40745_Track-79572: 575878

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40758_Track-80169: 154296; L40758_Track-79468: 300345

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP positive

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40733_Track-80136: 179227; L40733_Track-79352: 358719

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40725_Track-80213: 132022; L40725_Track-79607: 319318

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

text: normal

text: L40788_Track-79938: 174301; L40788_Track-79378: 346065

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP positive

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

characteristic: description

text: embryo

text: normal

text: L40747_Track-80027: 201867; L40747_Track-79447: 403163

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40765_Track-80182: 243513; L40765_Track-79410: 486725

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40804_Track-79647: 465931

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP positive

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40756_Track-79948: 120104; L40756_Track-79452: 237540

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40762_Track-80103: 108634; L40762_Track-79286: 213319

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

text: normal

text: L40742_Track-80059: 150900; L40742_Track-79280: 296768

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP positive

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

characteristic: description

text: embryo

text: normal

text: L40819_Track-80079: 206748; L40819_Track-79565: 415444

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40810_Track-80094: 9734; L40810_Track-79510: 17592

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40773_Track-80244: 441621; L40773_Track-79591: 1073399

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP positive

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40759_Track-79932: 305180; L40759_Track-79397: 610874

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: Protocols: hESC-derived pancreatic cells at Stage 4 Day 1 of the pancreatic differentiation protocol (Rezania et al., 2014; Petersen et al., 2017) were dissociated into single cells with TrypLE and resuspended into a FACS buffer containing 0.5% BSA and 2 mM EDTA (Thermo Fisher) in 1X PBS. The cells were index-sorted according to their GFP expression into 384-well plates on a BD FACSAria and snap-frozen. Cells are processed with SMARTseq-2 protocol miniaturized and modified based on Picelli et al. 2013 (doi: 10.1038/nmeth.2639). In short, cells are sorted in 0.5 µl 0.2% Triton X-100 solution with RNase Inhibitor into a 384-well plate, frozen at -80°C. After thawing, the cell lysate is mixed with 0.5 ul of a primer mix (containing 5 mM dNTP (Invitrogen), 0.5 uM dT-primer*, 1 U RNase Inhibitor (NEB)). RNA is denatured (3 minutes 72 °C), followed by reverse transcription (42 °C 90 min + 70°C 15 min) after adding 1.5 ul RT buffer mix (1x superscript II buffer (Invitrogen), 1 M betaine, 5 mM DTT, 6 mM MgCl2, 1 uM TSO-primer*, 2.3 U RNase Inhibitor and 25 U Superscript II). The cDNA is amplified using 1x Kapa HiFi HotStart Readymix (Roche) and 0.1 uM UP-primer under following cycling conditions: 98°C for 3 min, 23 cycles [98°C 20 sec, 67°C 15 sec, 72°C 6 min] and final elongation at 72°C for 5 min. The amplified cDNA is purified using 1x volume of Sera-Mag SpeedBeads (GE Healthcare) and eluted in 12 ul nuclease free water. For library preparation 1.5 µl cDNA is desiccated and rehydrated in 1 ul Tagmentation mix (1x TTBL buffer, 0.1 ul Tagment DNA Enzyme; from TruePrep DNA Library Prep Kit V2 for Illumina; Vazyme) and tagmented (55°C, 5 min), followed by an index PCR with 1x concentrated KAPA Hifi HotStart Ready Mix and 0.7 µM dual indexing primers (72°C 3 min, 98°C 30 sec, 13 cycles [98°C 10 sec, 63°C 20 sec, 72°C 1 min], 72°C 5 min). After PCR, libraries are quantified with AccuBlue Broad range chemistry (Biotium), equimolarly pooled and purified twice with 0.9x volume Sera-Mag SpeedBeads.

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40734_Track-80080: 106599; L40734_Track-79313: 140309

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

text: The Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

text: normal

text: L40807_Track-79956: 154075; L40807_Track-79405: 310193

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP positive

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

characteristic: description

text: embryo

text: normal

text: L40738_Track-80249: 42624; L40738_Track-79624: 59739

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40753_Track-80222: 117687; L40753_Track-79596: 284199

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40439_Track-80231: 51458; L40439_Track-79586: 124544

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP negative

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40510_Track-80220: 175064; L40510_Track-79633: 423159

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP negative

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40518_Track-80126: 182997; L40518_Track-79395: 363649

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP negative

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

text: normal

text: L40524_Track-80143: 168855; L40524_Track-79482: 339655

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP negative

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

characteristic: description

text: embryo

text: normal

text: L40822_Track-79664: 420470

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP negative

text: plate 0

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40825_Track-79666: 530454

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP negative

text: plate 0

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40462_Track-80225: 648460; L40462_Track-79626: 1582524

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP negative

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40805_Track-80142: 91827; L40805_Track-79385: 182647

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40493_Track-80000: 155512; L40493_Track-79381: 313148

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP negative

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

text: normal

text: L40470_Track-79990: 129503; L40470_Track-79368: 258829

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP negative

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

characteristic: description

text: embryo

text: normal

text: L40513_Track-80047: 155827; L40513_Track-79431: 310311

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP negative

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40488_Track-79951: 241557; L40488_Track-79350: 479863

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP negative

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40525_Track-80068: 171838; L40525_Track-79387: 344522

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP negative

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: Protocols: hESC-derived pancreatic cells at Stage 4 Day 1 of the pancreatic differentiation protocol (Rezania et al., 2014; Petersen et al., 2017) were dissociated into single cells with TrypLE and resuspended into a FACS buffer containing 0.5% BSA and 2 mM EDTA (Thermo Fisher) in 1X PBS. The cells were index-sorted according to their GFP expression into 384-well plates on a BD FACSAria and snap-frozen. Cells are processed with SMARTseq-2 protocol miniaturized and modified based on Picelli et al. 2013 (doi: 10.1038/nmeth.2639). In short, cells are sorted in 0.5 µl 0.2% Triton X-100 solution with RNase Inhibitor into a 384-well plate, frozen at -80°C. After thawing, the cell lysate is mixed with 0.5 ul of a primer mix (containing 5 mM dNTP (Invitrogen), 0.5 uM dT-primer*, 1 U RNase Inhibitor (NEB)). RNA is denatured (3 minutes 72 °C), followed by reverse transcription (42 °C 90 min + 70°C 15 min) after adding 1.5 ul RT buffer mix (1x superscript II buffer (Invitrogen), 1 M betaine, 5 mM DTT, 6 mM MgCl2, 1 uM TSO-primer*, 2.3 U RNase Inhibitor and 25 U Superscript II). The cDNA is amplified using 1x Kapa HiFi HotStart Readymix (Roche) and 0.1 uM UP-primer under following cycling conditions: 98°C for 3 min, 23 cycles [98°C 20 sec, 67°C 15 sec, 72°C 6 min] and final elongation at 72°C for 5 min. The amplified cDNA is purified using 1x volume of Sera-Mag SpeedBeads (GE Healthcare) and eluted in 12 ul nuclease free water. For library preparation 1.5 µl cDNA is desiccated and rehydrated in 1 ul Tagmentation mix (1x TTBL buffer, 0.1 ul Tagment DNA Enzyme; from TruePrep DNA Library Prep Kit V2 for Illumina; Vazyme) and tagmented (55°C, 5 min), followed by an index PCR with 1x concentrated KAPA Hifi HotStart Ready Mix and 0.7 µM dual indexing primers (72°C 3 min, 98°C 30 sec, 13 cycles [98°C 10 sec, 63°C 20 sec, 72°C 1 min], 72°C 5 min). After PCR, libraries are quantified with AccuBlue Broad range chemistry (Biotium), equimolarly pooled and purified twice with 0.9x volume Sera-Mag SpeedBeads.

characteristic: description

text: embryo

text: normal

text: L40443_Track-79645: 131216

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP negative

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40478_Track-80026: 216068; L40478_Track-79351: 434148

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP negative

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

text: The Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

text: normal

text: L40527_Track-80031: 225333; L40527_Track-79428: 451389

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP negative

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

characteristic: description

text: embryo

text: normal

text: L40506_Track-80196: 241832; L40506_Track-79400: 529262

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP negative

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40517_Track-80173: 193401; L40517_Track-79318: 389246

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP negative

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40447_Track-79935: 208930; L40447_Track-79404: 418247

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP negative

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40504_Track-80023: 264462; L40504_Track-79518: 527942

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP negative

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40526_Track-80180: 212186; L40526_Track-79319: 424947

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP negative

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

text: normal

text: L40446_Track-80030: 195293; L40446_Track-79402: 391771

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP negative

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

characteristic: description

text: embryo

text: normal

text: L40453_Track-80127: 224221; L40453_Track-79481: 452411

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP negative

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40514_Track-80202: 129250; L40514_Track-79422: 258128

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP negative

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40516_Track-79999: 167979; L40516_Track-79463: 336143

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP negative

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40823_Track-79673: 379511

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP negative

text: plate 0

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40466_Track-80270: 117560; L40466_Track-79643: 272575

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP negative

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

text: normal

text: L40511_Track-80266: 41576; L40511_Track-79595: 102187

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP negative

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

characteristic: description

text: embryo

text: normal

text: L40460_Track-80259: 254425; L40460_Track-79578: 618966

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP negative

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40497_Track-80082: 156262; L40497_Track-79326: 313496

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP negative

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: Protocols: hESC-derived pancreatic cells at Stage 4 Day 1 of the pancreatic differentiation protocol (Rezania et al., 2014; Petersen et al., 2017) were dissociated into single cells with TrypLE and resuspended into a FACS buffer containing 0.5% BSA and 2 mM EDTA (Thermo Fisher) in 1X PBS. The cells were index-sorted according to their GFP expression into 384-well plates on a BD FACSAria and snap-frozen. Cells are processed with SMARTseq-2 protocol miniaturized and modified based on Picelli et al. 2013 (doi: 10.1038/nmeth.2639). In short, cells are sorted in 0.5 µl 0.2% Triton X-100 solution with RNase Inhibitor into a 384-well plate, frozen at -80°C. After thawing, the cell lysate is mixed with 0.5 ul of a primer mix (containing 5 mM dNTP (Invitrogen), 0.5 uM dT-primer*, 1 U RNase Inhibitor (NEB)). RNA is denatured (3 minutes 72 °C), followed by reverse transcription (42 °C 90 min + 70°C 15 min) after adding 1.5 ul RT buffer mix (1x superscript II buffer (Invitrogen), 1 M betaine, 5 mM DTT, 6 mM MgCl2, 1 uM TSO-primer*, 2.3 U RNase Inhibitor and 25 U Superscript II). The cDNA is amplified using 1x Kapa HiFi HotStart Readymix (Roche) and 0.1 uM UP-primer under following cycling conditions: 98°C for 3 min, 23 cycles [98°C 20 sec, 67°C 15 sec, 72°C 6 min] and final elongation at 72°C for 5 min. The amplified cDNA is purified using 1x volume of Sera-Mag SpeedBeads (GE Healthcare) and eluted in 12 ul nuclease free water. For library preparation 1.5 µl cDNA is desiccated and rehydrated in 1 ul Tagmentation mix (1x TTBL buffer, 0.1 ul Tagment DNA Enzyme; from TruePrep DNA Library Prep Kit V2 for Illumina; Vazyme) and tagmented (55°C, 5 min), followed by an index PCR with 1x concentrated KAPA Hifi HotStart Ready Mix and 0.7 µM dual indexing primers (72°C 3 min, 98°C 30 sec, 13 cycles [98°C 10 sec, 63°C 20 sec, 72°C 1 min], 72°C 5 min). After PCR, libraries are quantified with AccuBlue Broad range chemistry (Biotium), equimolarly pooled and purified twice with 0.9x volume Sera-Mag SpeedBeads.

text: embryo

text: normal

text: L40454_Track-80074: 255872; L40454_Track-79529: 517473

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP negative

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title


characteristic: External Id

text: The Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40459_Track-80258: 377164; L40459_Track-79592: 906260

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP negative

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40490_Track-80063: 129871; L40490_Track-79524: 260462

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP negative

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

text: normal

text: L40480_Track-80100: 172452; L40480_Track-79484: 347042

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP negative

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1

characteristic: title