characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR209484 of study ERP002061.

characteristic: description

text: Host-associated

text: Human

text: Digestive system

text: Denmark

text: metagenome-assembled genome

text: human gut metagenome

text: human gut metagenome

text: uncultured Tenericutes bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Tenericutes bacterium

text: Illumina Genome Analyzer IIx

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR209484_bin.33_CONCOCT_v1.1_MAG

characteristic: title


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR209509 of study ERP002061.

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Denmark

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

characteristic: metagenomic source

text: uncultured Parabacteroides sp.

text: The microbial diversity of environments like the human gut extends far beyond what is covered by reference genomes. Here we present a method for exhaustive and unsupervised co-abundance gene binning across a series of highly complex metagenomic samples. As the method does not rely on previously sequenced reference genomes it allows for discovery of new species, viruses and clonal heterogeneity. We demonstrate the method on human gut microbiome data and identify 7,381 co-abundance gene groups (CAGs) ranging in size from 3 to 6,319 genes. The CAGs represent a wide variety of biological entities including microbial genomes, phages and clonal differences. We name the 741 largest of these metagenomic species (MGS), because they correspond to microbial species. In addition, we establish microbial host affiliations by dependency-associations for many small CAGs. Longitudinal sampling in the same individuals indicates that some of these dependency-associations are important for the persistence of their microbial host

characteristic: project name

text: uncultured Parabacteroides sp.

text: Illumina Genome Analyzer IIx

text: multi-marker approach

text: Metagenome-assembled genome: ERR209509_bin.0_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR209519 of study ERP002061.

characteristic: description

text: Host-associated

text: Human

text: Digestive system

text: Denmark

text: metagenome-assembled genome

text: human gut metagenome

text: human gut metagenome

text: uncultured Candidatus Melainabacteria bacterium

characteristic: organism

characteristic: sample derived from

text: Illumina Genome Analyzer IIx

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR209519_bin.73_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR209536 of study ERP002061.

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

characteristic: metagenomic source

text: uncultured Porphyromonas sp.

characteristic: project name

text: uncultured Porphyromonas sp.

text: Illumina Genome Analyzer IIx

text: multi-marker approach

text: Metagenome-assembled genome: ERR209536_bin.31_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR209569 of study ERP002061.

characteristic: description

text: Host-associated

text: Human

text: Digestive system

text: Spain

text: metagenome-assembled genome

text: human gut metagenome

text: human gut metagenome

text: Helicobacter winghamensis

characteristic: organism

characteristic: sample derived from

text: Helicobacter winghamensis

text: Illumina Genome Analyzer IIx

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR209569_bin.10_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR209574 of study ERP002061.

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

characteristic: metagenomic source

text: uncultured Phascolarctobacterium sp.

characteristic: project name

text: uncultured Phascolarctobacterium sp.

text: Illumina Genome Analyzer IIx

text: multi-marker approach

text: Metagenome-assembled genome: ERR209574_bin.37_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR209578 of study ERP002061.

characteristic: description

text: Host-associated

text: Human

text: Digestive system

text: Spain

text: metagenome-assembled genome

text: human gut metagenome

text: human gut metagenome

text: uncultured Mollicutes bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Mollicutes bacterium

text: Illumina Genome Analyzer IIx

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR209578_bin.64_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR209581 of study ERP002061.

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

characteristic: metagenomic source

text: uncultured Ruminococcus sp.

characteristic: project name

text: uncultured Ruminococcus sp.

text: Illumina Genome Analyzer IIx

text: multi-marker approach

text: Metagenome-assembled genome: ERR209581_bin.13_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR209583 of study ERP002061.

characteristic: description

text: Host-associated

text: Human

text: Digestive system

text: Spain

text: metagenome-assembled genome

text: human gut metagenome

text: human gut metagenome

text: uncultured Firmicutes bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Firmicutes bacterium

text: Illumina Genome Analyzer IIx

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR209583_bin.64_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR209654 of study ERP002061.

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

text: The microbial diversity of environments like the human gut extends far beyond what is covered by reference genomes. Here we present a method for exhaustive and unsupervised co-abundance gene binning across a series of highly complex metagenomic samples. As the method does not rely on previously sequenced reference genomes it allows for discovery of new species, viruses and clonal heterogeneity. We demonstrate the method on human gut microbiome data and identify 7,381 co-abundance gene groups (CAGs) ranging in size from 3 to 6,319 genes. The CAGs represent a wide variety of biological entities including microbial genomes, phages and clonal differences. We name the 741 largest of these metagenomic species (MGS), because they correspond to microbial species. In addition, we establish microbial host affiliations by dependency-associations for many small CAGs. Longitudinal sampling in the same individuals indicates that some of these dependency-associations are important for the persistence of their microbial host

characteristic: project name

text: uncultured Clostridia bacterium

text: Illumina Genome Analyzer IIx

text: multi-marker approach

text: Metagenome-assembled genome: ERR209654_bin.42_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR209703 of study ERP002061.

characteristic: description

text: Host-associated

text: Human

text: Digestive system

text: Spain

text: metagenome-assembled genome

text: human gut metagenome

text: human gut metagenome

text: uncultured Collinsella sp.

characteristic: organism

characteristic: sample derived from

text: uncultured Collinsella sp.

text: Illumina Genome Analyzer IIx

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR209703_bin.5_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

characteristic: metagenomic source

text: uncultured Bacteroides sp.

characteristic: project name

text: uncultured Bacteroides sp.

text: Illumina Genome Analyzer IIx

text: multi-marker approach

text: Metagenome-assembled genome: ERR209703_bin.51_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR209713 of study ERP002061.

characteristic: description

text: Host-associated

text: Human

text: Digestive system

text: Spain

text: metagenome-assembled genome

text: human gut metagenome

text: human gut metagenome

text: uncultured Megasphaera sp.

characteristic: organism

characteristic: sample derived from

text: uncultured Megasphaera sp.

text: Illumina Genome Analyzer IIx

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR209713_bin.61_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR209721 of study ERP002061.

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

characteristic: metagenomic source

text: Faecalibacterium prausnitzii

characteristic: project name

text: Faecalibacterium prausnitzii

text: Illumina Genome Analyzer IIx

text: multi-marker approach

text: Metagenome-assembled genome: ERR209721_bin.0_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR209782 of study ERP002061.

characteristic: description

text: Host-associated

text: Human

text: Digestive system

text: Spain

text: metagenome-assembled genome

text: human gut metagenome

text: human gut metagenome

text: Peptoniphilus senegalensis

characteristic: organism

characteristic: sample derived from

text: Peptoniphilus senegalensis

text: Illumina Genome Analyzer IIx

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR209782_bin.1_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: project name

text: uncultured Clostridia bacterium

text: Illumina Genome Analyzer IIx

text: multi-marker approach

text: Metagenome-assembled genome: ERR209782_bin.6_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR209783 of study ERP002061.

characteristic: description

text: Host-associated

text: Human

text: Digestive system

text: Spain

text: metagenome-assembled genome

text: human gut metagenome

text: human gut metagenome

text: uncultured Clostridia bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina Genome Analyzer IIx

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR209783_bin.59_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

characteristic: metagenomic source

text: uncultured Firmicutes bacterium

characteristic: project name

text: uncultured Firmicutes bacterium

text: Illumina Genome Analyzer IIx

text: multi-marker approach

text: Metagenome-assembled genome: ERR209783_bin.9_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR209807 of study ERP002061.

characteristic: description

text: Host-associated

text: Human

text: Digestive system

text: Spain

text: metagenome-assembled genome

text: human gut metagenome

text: human gut metagenome

text: uncultured Porphyromonas sp.

characteristic: organism

characteristic: sample derived from

text: uncultured Porphyromonas sp.

text: Illumina Genome Analyzer IIx

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR209807_bin.28_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

characteristic: metagenomic source

text: uncultured Olsenella sp.

text: The microbial diversity of environments like the human gut extends far beyond what is covered by reference genomes. Here we present a method for exhaustive and unsupervised co-abundance gene binning across a series of highly complex metagenomic samples. As the method does not rely on previously sequenced reference genomes it allows for discovery of new species, viruses and clonal heterogeneity. We demonstrate the method on human gut microbiome data and identify 7,381 co-abundance gene groups (CAGs) ranging in size from 3 to 6,319 genes. The CAGs represent a wide variety of biological entities including microbial genomes, phages and clonal differences. We name the 741 largest of these metagenomic species (MGS), because they correspond to microbial species. In addition, we establish microbial host affiliations by dependency-associations for many small CAGs. Longitudinal sampling in the same individuals indicates that some of these dependency-associations are important for the persistence of their microbial host

characteristic: project name

text: uncultured Olsenella sp.

text: Illumina Genome Analyzer IIx

text: multi-marker approach

text: Metagenome-assembled genome: ERR209807_bin.42_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR209808 of study ERP002061.

characteristic: description

text: Host-associated

text: Human

text: Digestive system

text: Spain

text: metagenome-assembled genome

text: human gut metagenome

text: human gut metagenome

text: uncultured Parvimonas sp.

characteristic: organism

characteristic: sample derived from

text: uncultured Parvimonas sp.

text: Illumina Genome Analyzer IIx

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR209808_bin.33_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR209832 of study ERP002061.

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

characteristic: metagenomic source

text: uncultured Tenericutes bacterium

characteristic: project name

text: uncultured Tenericutes bacterium

text: Illumina Genome Analyzer IIx

text: multi-marker approach

text: Metagenome-assembled genome: ERR209832_bin.33_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Human

text: Digestive system

text: Spain

text: metagenome-assembled genome

text: human gut metagenome

text: human gut metagenome

text: uncultured Mollicutes bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Mollicutes bacterium

text: Illumina Genome Analyzer IIx

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR209832_bin.64_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR209878 of study ERP002061.

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

characteristic: metagenomic source

text: uncultured Prevotella sp.

characteristic: project name

text: uncultured Prevotella sp.

text: Illumina Genome Analyzer IIx

text: multi-marker approach

text: Metagenome-assembled genome: ERR209878_bin.16_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR209884 of study ERP002061.

characteristic: description

text: Host-associated

text: Human

text: Digestive system

text: Spain

text: metagenome-assembled genome

text: human gut metagenome

text: human gut metagenome

text: uncultured Ruminococcus sp.

characteristic: organism

characteristic: sample derived from

text: uncultured Ruminococcus sp.

text: Illumina Genome Analyzer IIx

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR209884_bin.41_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes v3.12.0

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR2198611 of study ERP105282.

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

text: The metadata for this study is available on Dropbox:dropbox [DOT] com/sh/kfiu4wb53oemn6j/AAB13sgaKS7MV4lVqXLCxTuMa?dl=0Abstract: Fecal microbiota transplantation (FMT) is a treatment for microbiome-associated diseases in which gut microbiota are transferred from a healthy donor to a patient. Although the success of FMT requires donor bacteria to engraft in the patient's gut, the forces governing bacterial engraftment in humans are unknown. Here, we use a vast, ongoing clinical experiment - the treatment of recurrent Clostridium difficile infection with FMT - to uncover the rules of engraftment in humans. First, we built a machine learning model that accurately predicts which bacterial species will engraft in a given host. We then developed a maximum-likelihood strain inference method, Strain Finder, allowing us to infer the genotypes of donor strains and to track them through patients' guts over time. Surprisingly, engraftment could be predicted largely from the abundance and phylogeny of bacteria in the donor and the pre-FMT patient. We also found that donor strains within a species engraft in an all-or-nothing manner and that previously undetected strains frequently colonize the patient after FMT. We validated these findings in another disease context, metabolic syndrome, suggesting that the same principles of engraftment extend to other indications. These findings may guide the design of bacterial therapeutics that target diseases ranging from ulcerative colitis to cancer.

characteristic: project name

text: uncultured Clostridia bacterium

text: Illumina Genome Analyzer IIx

text: multi-marker approach

text: Metagenome-assembled genome: ERR2198611_bin.101_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.12.0

characteristic: assembly software

text: Default

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text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Human

text: Digestive system

text: USA

text: metagenome-assembled genome

text: human gut metagenome

text: human gut metagenome

text: Lachnoclostridium phocaeense

characteristic: organism

characteristic: sample derived from

text: Lachnoclostridium phocaeense

text: Illumina Genome Analyzer IIx

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR2198611_bin.2_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.12.0

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

characteristic: metagenomic source

text: uncultured Butyricicoccus sp.

characteristic: project name

text: uncultured Butyricicoccus sp.

text: Illumina Genome Analyzer IIx

text: multi-marker approach

text: Metagenome-assembled genome: ERR2198611_bin.27_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.12.0

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Human

text: Digestive system

text: USA

text: metagenome-assembled genome

text: human gut metagenome

text: human gut metagenome

text: uncultured Alistipes sp.

characteristic: organism

characteristic: sample derived from

text: uncultured Alistipes sp.

text: Illumina Genome Analyzer IIx

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR2198611_bin.70_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.12.0

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

characteristic: metagenomic source

text: uncultured Dialister sp.

characteristic: project name

text: uncultured Dialister sp.

text: Illumina Genome Analyzer IIx

text: multi-marker approach

text: Metagenome-assembled genome: ERR2198611_bin.87_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

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characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.12.0

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR2198616 of study ERP105282.

characteristic: description

text: Host-associated

text: Human

text: Digestive system

text: USA

text: metagenome-assembled genome

text: human gut metagenome

text: human gut metagenome

text: Faecalibacterium prausnitzii

characteristic: organism

characteristic: sample derived from

text: Faecalibacterium prausnitzii

text: Illumina Genome Analyzer IIx

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR2198616_bin.106_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.12.0

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

characteristic: metagenomic source

text: Emergencia timonensis

characteristic: project name

text: Emergencia timonensis

text: Illumina Genome Analyzer IIx

text: multi-marker approach

text: Metagenome-assembled genome: ERR2198616_bin.93_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.12.0

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR2198617 of study ERP105282.

characteristic: description

text: Host-associated

text: Human

text: Digestive system

text: USA

text: metagenome-assembled genome

text: human gut metagenome

text: human gut metagenome

text: Enterococcus dispar

characteristic: organism

characteristic: sample derived from

text: Enterococcus dispar

text: Illumina Genome Analyzer IIx

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR2198617_bin.46_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.12.0

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

characteristic: metagenomic source

text: uncultured Acidaminococcus sp.

characteristic: project name

text: uncultured Acidaminococcus sp.

text: Illumina Genome Analyzer IIx

text: multi-marker approach

text: Metagenome-assembled genome: ERR2198617_bin.62_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.12.0

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Human

text: Digestive system

text: USA

text: metagenome-assembled genome

text: human gut metagenome

text: human gut metagenome

text: uncultured Leuconostoc sp.

characteristic: organism

characteristic: sample derived from

text: uncultured Leuconostoc sp.

text: Illumina Genome Analyzer IIx

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR2198617_bin.63_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.12.0

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

characteristic: metagenomic source

text: Klebsiella oxytoca

characteristic: project name

text: Klebsiella oxytoca

text: Illumina Genome Analyzer IIx

text: multi-marker approach

text: Metagenome-assembled genome: ERR2198617_bin.70_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.12.0

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Human

text: Digestive system

text: USA

text: metagenome-assembled genome

text: human gut metagenome

text: human gut metagenome

text: uncultured Succinatimonas sp.

characteristic: organism

characteristic: sample derived from

text: uncultured Succinatimonas sp.

text: Illumina Genome Analyzer IIx

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR2198617_bin.74_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes v3.12.0

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR2198621 of study ERP105282.

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

characteristic: metagenomic source

text: Isoptericola variabilis

characteristic: project name

text: Isoptericola variabilis

text: Illumina Genome Analyzer IIx

text: multi-marker approach

text: Metagenome-assembled genome: ERR2198621_bin.100_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.12.0

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Human

text: Digestive system

text: USA

text: metagenome-assembled genome

text: human gut metagenome

text: human gut metagenome

text: uncultured Coriobacteriaceae bacterium

characteristic: organism

text: The metadata for this study is available on Dropbox:dropbox [DOT] com/sh/kfiu4wb53oemn6j/AAB13sgaKS7MV4lVqXLCxTuMa?dl=0Abstract: Fecal microbiota transplantation (FMT) is a treatment for microbiome-associated diseases in which gut microbiota are transferred from a healthy donor to a patient. Although the success of FMT requires donor bacteria to engraft in the patient's gut, the forces governing bacterial engraftment in humans are unknown. Here, we use a vast, ongoing clinical experiment - the treatment of recurrent Clostridium difficile infection with FMT - to uncover the rules of engraftment in humans. First, we built a machine learning model that accurately predicts which bacterial species will engraft in a given host. We then developed a maximum-likelihood strain inference method, Strain Finder, allowing us to infer the genotypes of donor strains and to track them through patients' guts over time. Surprisingly, engraftment could be predicted largely from the abundance and phylogeny of bacteria in the donor and the pre-FMT patient. We also found that donor strains within a species engraft in an all-or-nothing manner and that previously undetected strains frequently colonize the patient after FMT. We validated these findings in another disease context, metabolic syndrome, suggesting that the same principles of engraftment extend to other indications. These findings may guide the design of bacterial therapeutics that target diseases ranging from ulcerative colitis to cancer.

characteristic: sample derived from

text: uncultured Coriobacteriaceae bacterium

text: Illumina Genome Analyzer IIx

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR2198621_bin.120_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.12.0

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR2198623 of study ERP105282.

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: project name

text: uncultured Clostridia bacterium

text: Illumina Genome Analyzer IIx

text: multi-marker approach

text: Metagenome-assembled genome: ERR2198623_bin.1_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.12.0

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Human

text: Digestive system

text: USA

text: metagenome-assembled genome

text: human gut metagenome

text: human gut metagenome

text: uncultured Desulfovibrio sp.

characteristic: organism

characteristic: sample derived from

text: uncultured Desulfovibrio sp.

text: Illumina Genome Analyzer IIx

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR2198623_bin.120_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.12.0

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

characteristic: metagenomic source

text: uncultured Blautia sp.

characteristic: project name

text: uncultured Blautia sp.

text: Illumina Genome Analyzer IIx

text: multi-marker approach

text: Metagenome-assembled genome: ERR2198623_bin.124_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.12.0

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Human

text: Digestive system

text: USA

text: metagenome-assembled genome

text: human gut metagenome

text: human gut metagenome

text: Anaerofustis stercorihominis

characteristic: organism

characteristic: sample derived from

text: Anaerofustis stercorihominis

text: Illumina Genome Analyzer IIx

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR2198623_bin.17_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.12.0

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

characteristic: metagenomic source

text: uncultured Lachnospiraceae bacterium

characteristic: project name

text: uncultured Lachnospiraceae bacterium

text: Illumina Genome Analyzer IIx

text: multi-marker approach

text: Metagenome-assembled genome: ERR2198623_bin.28_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.12.0

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Human

text: Digestive system

text: USA

text: metagenome-assembled genome

text: human gut metagenome

text: human gut metagenome

text: uncultured Eubacterium sp.

characteristic: organism

characteristic: sample derived from

text: uncultured Eubacterium sp.

text: Illumina Genome Analyzer IIx

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR2198623_bin.52_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.12.0

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

characteristic: metagenomic source

text: uncultured Eubacteriaceae bacterium

characteristic: project name

text: uncultured Eubacteriaceae bacterium

text: Illumina Genome Analyzer IIx

text: multi-marker approach

text: Metagenome-assembled genome: ERR2198623_bin.7_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.12.0

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Human

text: Digestive system

text: USA

text: metagenome-assembled genome

text: human gut metagenome

text: human gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: Illumina Genome Analyzer IIx

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR2198623_bin.84_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.12.0

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: project name

text: uncultured Clostridia bacterium

text: Illumina Genome Analyzer IIx

text: multi-marker approach

text: Metagenome-assembled genome: ERR2198623_bin.89_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.12.0

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR2198624 of study ERP105282.

characteristic: description

text: Host-associated

text: Human

text: Digestive system

text: USA

text: metagenome-assembled genome

text: human gut metagenome

text: human gut metagenome

text: uncultured Clostridia bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina Genome Analyzer IIx

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR2198624_bin.115_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes v3.12.0

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

characteristic: metagenomic source

text: uncultured Erysipelotrichaceae bacterium

characteristic: project name

text: Illumina Genome Analyzer IIx

text: multi-marker approach

text: Metagenome-assembled genome: ERR2198624_bin.148_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.12.0

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Human

text: Digestive system

text: USA

text: metagenome-assembled genome

text: human gut metagenome

text: human gut metagenome

text: uncultured Clostridia bacterium

characteristic: organism

text: The metadata for this study is available on Dropbox:dropbox [DOT] com/sh/kfiu4wb53oemn6j/AAB13sgaKS7MV4lVqXLCxTuMa?dl=0Abstract: Fecal microbiota transplantation (FMT) is a treatment for microbiome-associated diseases in which gut microbiota are transferred from a healthy donor to a patient. Although the success of FMT requires donor bacteria to engraft in the patient's gut, the forces governing bacterial engraftment in humans are unknown. Here, we use a vast, ongoing clinical experiment - the treatment of recurrent Clostridium difficile infection with FMT - to uncover the rules of engraftment in humans. First, we built a machine learning model that accurately predicts which bacterial species will engraft in a given host. We then developed a maximum-likelihood strain inference method, Strain Finder, allowing us to infer the genotypes of donor strains and to track them through patients' guts over time. Surprisingly, engraftment could be predicted largely from the abundance and phylogeny of bacteria in the donor and the pre-FMT patient. We also found that donor strains within a species engraft in an all-or-nothing manner and that previously undetected strains frequently colonize the patient after FMT. We validated these findings in another disease context, metabolic syndrome, suggesting that the same principles of engraftment extend to other indications. These findings may guide the design of bacterial therapeutics that target diseases ranging from ulcerative colitis to cancer.

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina Genome Analyzer IIx

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR2198624_bin.151_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.12.0

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: project name

text: uncultured Clostridia bacterium

text: Illumina Genome Analyzer IIx

text: multi-marker approach

text: Metagenome-assembled genome: ERR2198624_bin.20_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.12.0

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Human

text: Digestive system

text: USA

text: metagenome-assembled genome

text: human gut metagenome

text: human gut metagenome

text: uncultured Clostridia bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina Genome Analyzer IIx

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR2198624_bin.33_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.12.0

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

characteristic: metagenomic source

text: uncultured Acidiphilium sp.

characteristic: project name

text: uncultured Acidiphilium sp.

text: Illumina Genome Analyzer IIx

text: multi-marker approach

text: Metagenome-assembled genome: ERR2198624_bin.59_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.12.0

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Human

text: Digestive system

text: USA

text: metagenome-assembled genome

text: human gut metagenome

text: human gut metagenome

text: uncultured Blautia sp.

characteristic: organism

characteristic: sample derived from

text: uncultured Blautia sp.

text: Illumina Genome Analyzer IIx

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR2198624_bin.78_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.12.0

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR2198626 of study ERP105282.

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

characteristic: metagenomic source

text: uncultured Lachnoclostridium sp.

characteristic: project name

text: uncultured Lachnoclostridium sp.

text: Illumina Genome Analyzer IIx

text: multi-marker approach

text: Metagenome-assembled genome: ERR2198626_bin.22_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.12.0

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Human

text: Digestive system

text: USA

text: metagenome-assembled genome

text: human gut metagenome

text: human gut metagenome

text: uncultured Desulfovibrio sp.

characteristic: organism

characteristic: sample derived from

text: uncultured Desulfovibrio sp.

text: Illumina Genome Analyzer IIx

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR2198626_bin.77_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.12.0

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

characteristic: metagenomic source

text: Enterococcus raffinosus

characteristic: project name

text: Enterococcus raffinosus

text: Illumina Genome Analyzer IIx

text: multi-marker approach

text: Metagenome-assembled genome: ERR2198626_bin.9_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.12.0

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR2198629 of study ERP105282.

characteristic: description

text: Host-associated

text: Human

text: Digestive system

text: USA

text: metagenome-assembled genome

text: human gut metagenome

text: human gut metagenome

text: uncultured Mollicutes bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Mollicutes bacterium

text: Illumina Genome Analyzer IIx

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR2198629_bin.100_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.12.0

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

characteristic: metagenomic source

text: Lawsonibacter asaccharolyticus

characteristic: project name

text: Lawsonibacter asaccharolyticus

text: Illumina Genome Analyzer IIx

text: multi-marker approach

text: Metagenome-assembled genome: ERR2198629_bin.111_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.12.0

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Human

text: Digestive system

text: USA

text: metagenome-assembled genome

text: human gut metagenome

text: human gut metagenome

text: uncultured Firmicutes bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Firmicutes bacterium

text: Illumina Genome Analyzer IIx

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR2198629_bin.20_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes v3.12.0

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: project name

text: uncultured Clostridia bacterium

text: Illumina Genome Analyzer IIx

text: multi-marker approach

text: Metagenome-assembled genome: ERR2198629_bin.23_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.12.0

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Human

text: Digestive system

text: USA

text: metagenome-assembled genome

text: human gut metagenome

text: human gut metagenome

text: uncultured Clostridia bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina Genome Analyzer IIx

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR2198629_bin.92_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.12.0

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR2198631 of study ERP105282.

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

text: The metadata for this study is available on Dropbox:dropbox [DOT] com/sh/kfiu4wb53oemn6j/AAB13sgaKS7MV4lVqXLCxTuMa?dl=0Abstract: Fecal microbiota transplantation (FMT) is a treatment for microbiome-associated diseases in which gut microbiota are transferred from a healthy donor to a patient. Although the success of FMT requires donor bacteria to engraft in the patient's gut, the forces governing bacterial engraftment in humans are unknown. Here, we use a vast, ongoing clinical experiment - the treatment of recurrent Clostridium difficile infection with FMT - to uncover the rules of engraftment in humans. First, we built a machine learning model that accurately predicts which bacterial species will engraft in a given host. We then developed a maximum-likelihood strain inference method, Strain Finder, allowing us to infer the genotypes of donor strains and to track them through patients' guts over time. Surprisingly, engraftment could be predicted largely from the abundance and phylogeny of bacteria in the donor and the pre-FMT patient. We also found that donor strains within a species engraft in an all-or-nothing manner and that previously undetected strains frequently colonize the patient after FMT. We validated these findings in another disease context, metabolic syndrome, suggesting that the same principles of engraftment extend to other indications. These findings may guide the design of bacterial therapeutics that target diseases ranging from ulcerative colitis to cancer.

characteristic: project name

text: uncultured Clostridia bacterium

text: Illumina Genome Analyzer IIx

text: multi-marker approach

text: Metagenome-assembled genome: ERR2198631_bin.107_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.12.0

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Human

text: Digestive system

text: USA

text: metagenome-assembled genome

text: human gut metagenome

text: human gut metagenome

text: uncultured Clostridia bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina Genome Analyzer IIx

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR2198631_bin.112_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.12.0

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

characteristic: metagenomic source

text: Veillonella parvula

characteristic: project name

text: Veillonella parvula

text: Illumina Genome Analyzer IIx

text: multi-marker approach

text: Metagenome-assembled genome: ERR2198631_bin.113_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.12.0

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Human

text: Digestive system

text: USA

text: metagenome-assembled genome

text: human gut metagenome

text: human gut metagenome

text: uncultured Solobacterium sp.

characteristic: organism

characteristic: sample derived from

text: uncultured Solobacterium sp.

text: Illumina Genome Analyzer IIx

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR2198631_bin.27_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.12.0

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

characteristic: metagenomic source

text: Dorea longicatena

characteristic: project name

text: Dorea longicatena

text: Illumina Genome Analyzer IIx

text: multi-marker approach

text: Metagenome-assembled genome: ERR2198631_bin.28_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.12.0

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Human

text: Digestive system

text: USA

text: metagenome-assembled genome

text: human gut metagenome

text: human gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: Illumina Genome Analyzer IIx

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR2198631_bin.37_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.12.0

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

characteristic: metagenomic source

text: uncultured Lactobacillus sp.

characteristic: project name

text: uncultured Lactobacillus sp.

text: Illumina Genome Analyzer IIx

text: multi-marker approach

text: Metagenome-assembled genome: ERR2198631_bin.53_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.12.0

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Human

text: Digestive system

text: USA

text: metagenome-assembled genome

text: human gut metagenome

text: human gut metagenome

text: uncultured Eggerthella sp.

characteristic: organism

characteristic: sample derived from

text: uncultured Eggerthella sp.

text: Illumina Genome Analyzer IIx

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR2198631_bin.55_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.12.0

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR2198632 of study ERP105282.

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

characteristic: metagenomic source

text: Streptococcus gordonii

characteristic: project name

text: Streptococcus gordonii

text: Illumina Genome Analyzer IIx

text: multi-marker approach

text: Metagenome-assembled genome: ERR2198632_bin.80_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.12.0

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR2198634 of study ERP105282.

characteristic: description

text: Host-associated

text: Human

text: Digestive system

text: USA

text: metagenome-assembled genome

text: human gut metagenome

text: human gut metagenome

text: uncultured Clostridia bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina Genome Analyzer IIx

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR2198634_bin.71_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes v3.12.0

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR2198636 of study ERP105282.

text: Host-associated

characteristic: environment (biome)

text: Human

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

characteristic: metagenomic source

text: uncultured Clostridium sp.

characteristic: project name

text: uncultured Clostridium sp.

text: Illumina Genome Analyzer IIx

text: multi-marker approach

text: Metagenome-assembled genome: ERR2198636_bin.57_CONCOCT_v1.1_MAG