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text: human

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text: Protocols: For Reversine/DMSO treatment: hTERT RPE-1 cells were synchronized with a single thymidine block. After thymidine release, synchronized cells were treated with DMSO or 500 nM Reversine for 24 hours. After Reversine/DMSO washout, cells were allowed to divide for about 60 hours. Then, they were either harvested or incubated with the fluorescent substrate of the B-galactosidase enzyme DDAO-Galactoside (DDAOG) 10 mM for 90 minutes. At the end of the incubation, cells were harvested for FACS sorting and acquired using a FACSAria Fusion flow cytometer (BD). Cells were gated for singlets and alive cells and then FSC-A, SSC-A and Alexa-647 intensity were used to distinguish cycling from arrested cells, i.e. cycling cells were gated using the same Alexa-647 Mean Fluorescence Intensity of the control sample without DDAOG, while arrested cells were gated imposing 0,1% on Alexa-647 signal to the control sample without DDAOG. For whole-transcriptome analysis, RNA was isolated using the RNeasy mini kit (Qiagen) in accordance to manufacturer's protocol and RNA quality was examined using the Agilent 2100 Bioanalyzer (Agilent Technologies). 500 ng of total RNA was used as input for library preparation using TruSeq total stranded RNA Illumina.

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: retinal pigment epithelium

characteristic: organism part

text: Homo sapiens

text: Aneuploid Arrested Replicate 1


name: E-MTAB-12537:Aneuploid Arrested Replicate 2

text: European Research Institute for the Biology of Ageing

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: hTERT RPE-1

text: human

text: adult

text: normal

text: wild type genotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: retinal pigment epithelium

text: Homo sapiens

text: Aneuploid Arrested Replicate 2


name: E-MTAB-12537:Aneuploid Cycling Replicate 1

characteristic: ENA-CHECKLIST

text: public

text: ArrayExpress

text: hTERT RPE-1

text: human

characteristic: description

text: adult

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: retinal pigment epithelium

text: Homo sapiens

text: Aneuploid Cycling Replicate 1

characteristic: title


name: E-MTAB-12537:Aneuploid Cycling Replicate 2

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: ArrayExpress

text: hTERT RPE-1

text: human

text: adult

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: retinal pigment epithelium

text: Homo sapiens

text: Aneuploid Cycling Replicate 2


E-MTAB-12537:Aneuploid Replicate 1

text: public

text: E-MTAB-12537:Aneuploid Replicate 1

text: ArrayExpress

characteristic: broker name

text: hTERT RPE-1

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: retinal pigment epithelium

text: Homo sapiens

text: Aneuploid Replicate 1


E-MTAB-12537:Aneuploid Replicate 2

text: public

text: E-MTAB-12537:Aneuploid Replicate 2

text: ArrayExpress

text: hTERT RPE-1

text: human

text: adult

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: retinal pigment epithelium

characteristic: organism part

text: Homo sapiens

text: Aneuploid Replicate 2


name: E-MTAB-12537:Pseudo-diploid Control Replicate 1

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: hTERT RPE-1

text: human

text: adult

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: retinal pigment epithelium

text: Homo sapiens

text: Pseudo-diploid Control Replicate 1


name: E-MTAB-12537:Pseudo-diploid Control Replicate 2

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: hTERT RPE-1

text: human

text: adult

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: retinal pigment epithelium

text: Homo sapiens

text: Pseudo-diploid Control Replicate 2


characteristic: ENA-CHECKLIST

text: NGS Competence Center Tuebingen NCCT

text: public

tag: Namespace:NGS Competence Center Tuebingen NCCT

text: isolate 1

characteristic: description

text: Nigeria

characteristic: geographic location (country and/or sea)

text: diseased

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: wound swab

text: Pseudomonas aeruginosa

characteristic: organism

text: Pseudomonas aeruginosa


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 2

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa

characteristic: title


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 3

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 4

text: Nigeria

text: diseased

text: Homo sapiens

text: urine

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 5

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 6

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 7

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 8

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 9

text: Nigeria

text: diseased

text: Homo sapiens

text: tissue sample

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 10

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 11

text: Nigeria

text: diseased

text: Homo sapiens

text: sputum

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 12

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 13

text: Nigeria

text: diseased

text: Homo sapiens

text: ear swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 14

text: Nigeria

text: diseased

text: Homo sapiens

text: tissue sample

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 15

text: Nigeria

text: diseased

text: Homo sapiens

text: sputum

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 16

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 17

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 18

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 19

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 20

text: Nigeria

text: diseased

text: Homo sapiens

text: catheter tip

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 21

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 22

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 23

text: Nigeria

text: diseased

text: Homo sapiens

text: urine

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 24

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 25

text: Nigeria

text: diseased

text: Homo sapiens

text: urine

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 26

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 27

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 28

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 29

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 30

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 31

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 32

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 33

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 34

text: Nigeria

text: diseased

text: Homo sapiens

text: urine

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 35

text: Nigeria

text: diseased

text: Homo sapiens

text: urine

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 36

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 37

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 38

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 39

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 40

text: Nigeria

text: diseased

text: Homo sapiens

text: urine

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 41

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 42

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 43

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 44

text: Nigeria

text: diseased

text: Homo sapiens

text: sputum

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 45

text: Nigeria

text: diseased

text: Homo sapiens

text: urine

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 46

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 47

text: Nigeria

text: diseased

text: Homo sapiens

text: urine

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 48

text: Nigeria

text: diseased

text: Homo sapiens

text: ear swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 49

text: Nigeria

text: diseased

text: Homo sapiens

text: urine

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 50

text: Nigeria

text: diseased

text: Homo sapiens

text: urine

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 51

text: Nigeria

text: diseased

text: Homo sapiens

text: urine

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 52

text: Nigeria

text: diseased

text: Homo sapiens

text: ear swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 53

text: Nigeria

text: diseased

text: Homo sapiens

text: ear swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 54

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 55

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 56

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 57

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 58

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 59

text: Nigeria

text: diseased

text: Homo sapiens

text: urine

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 60

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 61

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 62

text: Nigeria

text: diseased

text: Homo sapiens

text: catheter tip

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 63

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 64

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 65

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 66

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 67

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 68

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 69

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 70

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 71

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 72

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 73

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 74

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 75

text: Nigeria

text: diseased

text: Homo sapiens

text: urine

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 76

text: Nigeria

text: diseased

text: Homo sapiens

text: sputum

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 77

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 78

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: NGS Competence Center Tuebingen NCCT

text: public

text: isolate 79

text: Nigeria

text: diseased

text: Homo sapiens

text: wound swab

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa