characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR599165 of study ERP001736.

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: not provided

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: uncultured euryarchaeote

characteristic: organism

characteristic: sample derived from

text: uncultured euryarchaeote

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR599165_bin.110_CONCOCT_v1.1_MAG

characteristic: title


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: not provided

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured euryarchaeote

text: Seawater was filtered from different depths to retain small cell sizes (Bacteria Organisms). The DNA was extracted and submitted to high throughput sequencing.

characteristic: project name

text: uncultured euryarchaeote

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: ERR599165_bin.117_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: not provided

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: uncultured Alphaproteobacteria bacterium

characteristic: organism

characteristic: sample derived from

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR599165_bin.3_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: not provided

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured euryarchaeote

characteristic: project name

text: uncultured euryarchaeote

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: ERR599165_bin.4_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: not provided

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: uncultured Verrucomicrobiales bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Verrucomicrobiales bacterium

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR599165_bin.90_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: not provided

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured euryarchaeote

characteristic: project name

text: uncultured euryarchaeote

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: ERR599165_bin.92_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR599166 of study ERP001736.

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: not provided

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: uncultured euryarchaeote

characteristic: organism

characteristic: sample derived from

text: uncultured euryarchaeote

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR599166_bin.113_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: not provided

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured Rhodobacteraceae bacterium

characteristic: project name

text: uncultured Rhodobacteraceae bacterium

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: ERR599166_bin.139_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: not provided

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: uncultured euryarchaeote

characteristic: organism

characteristic: sample derived from

text: uncultured euryarchaeote

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR599166_bin.148_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: not provided

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured Aequorivita sp.

characteristic: project name

text: uncultured Aequorivita sp.

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: ERR599166_bin.164_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: not provided

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: uncultured bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured bacterium

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR599166_bin.18_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: not provided

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured Gammaproteobacteria bacterium

characteristic: project name

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: ERR599166_bin.21_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: not provided

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: uncultured Flavobacteriaceae bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Flavobacteriaceae bacterium

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR599166_bin.73_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: not provided

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured euryarchaeote

characteristic: project name

text: uncultured euryarchaeote

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: ERR599166_bin.80_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR599167 of study ERP001736.

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: not provided

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: uncultured Bdellovibrionaceae bacterium

characteristic: organism

text: Seawater was filtered from different depths to retain small cell sizes (Bacteria Organisms). The DNA was extracted and submitted to high throughput sequencing.

characteristic: sample derived from

text: uncultured Bdellovibrionaceae bacterium

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR599167_bin.112_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: not provided

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured euryarchaeote

characteristic: project name

text: uncultured euryarchaeote

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: ERR599167_bin.114_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: not provided

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: uncultured Rhodospirillaceae bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Rhodospirillaceae bacterium

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR599167_bin.128_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: not provided

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured euryarchaeote

characteristic: project name

text: uncultured euryarchaeote

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: ERR599167_bin.133_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: not provided

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: uncultured Desulfobacteraceae bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Desulfobacteraceae bacterium

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR599167_bin.136_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: not provided

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured Nitrospinae bacterium

characteristic: project name

text: uncultured Nitrospinae bacterium

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: ERR599167_bin.44_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: not provided

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: uncultured Rhodospirillaceae bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Rhodospirillaceae bacterium

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR599167_bin.64_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: not provided

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured bacterium

characteristic: project name

text: uncultured bacterium

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: ERR599167_bin.97_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR599169 of study ERP001736.

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: not provided

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: uncultured euryarchaeote

characteristic: organism

characteristic: sample derived from

text: uncultured euryarchaeote

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR599169_bin.29_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: not provided

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured Alphaproteobacteria bacterium

characteristic: project name

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: ERR599169_bin.38_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR599171 of study ERP001736.

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: not provided

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: Candidatus Nitrosopelagicus brevis

characteristic: organism

characteristic: sample derived from

text: Candidatus Nitrosopelagicus brevis

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR599171_bin.43_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR599172 of study ERP001736.

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: not provided

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured euryarchaeote

characteristic: project name

text: uncultured euryarchaeote

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: ERR599172_bin.16_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: not provided

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: uncultured bacterium

characteristic: organism

text: Seawater was filtered from different depths to retain small cell sizes (Bacteria Organisms). The DNA was extracted and submitted to high throughput sequencing.

characteristic: sample derived from

text: uncultured bacterium

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR599172_bin.55_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR599175 of study ERP001736.

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: not provided

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured Rhodospirillaceae bacterium

characteristic: project name

text: uncultured Rhodospirillaceae bacterium

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: ERR599175_bin.15_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR864073 of study ERP010213.

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: USA

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: uncultured Rhodobacteraceae bacterium

characteristic: organism

text: Microbial communities mediate the biogeochemical cycles that drive ecosystems, and it is important to understand how these communities are affected by changing environmental conditions, especially in complex coastal zones. As fresh and marine waters mix in estuaries and river plumes, the salinity, temperature, and nutrient gradients that are generated strongly influence bacterioplankton community structure, yet, a parallel shift in functional diversity has not been described. Metagenomic and metatranscriptomic analyses were conducted on five water samples spanning the salinity gradient of the Columbia River coastal margin, including river, estuary, plume, and ocean, in August 2010. Samples were pre-filtered through 3 μm filters and collected on 0.2 μm filters, thus results were focused on changes among free-living microbial communities. Results from metagenomic 16S rRNA sequences showed taxonomically distinct bacterial communities in river, estuary, and coastal ocean. Despite the strong salinity gradient observed over sampling locations (0 to 33), the functional gene profiles in the metagenomes were very similar from river to ocean with an average similarity of 82%. The metatranscriptomes, however, were an average of only 31% similar. We observed shifts from river to ocean in the abundance of genes encoding specific functions, such as catabolic pathways, osmoregulators, and metal transporters. Additionally, genes specifying both bacterial oxygenic and anoxygenic photosynthesis were highly abundant and expressed in the estuary and plume. Denitrification genes were found throughout the Columbia River coastal margin, and most highly expressed in the estuary. Across a river to ocean gradient, the free-living microbial community followed three different patterns of diversity: 1) the taxonomy of the community shifted strongly with salinity, 2) metabolic potential was highly similar across samples, with only small differences in functional gene abundance from river to ocean, and 3) gene expression was highly variable and generally was independent of changes in salinity.

characteristic: sample derived from

text: uncultured Rhodobacteraceae bacterium

text: Illumina HiSeq 1000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR864073_bin.111_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes version 3.10.139

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured Caulobacterales bacterium

characteristic: project name

text: uncultured Caulobacterales bacterium

text: Illumina HiSeq 1000

text: multi-marker approach

text: Metagenome-assembled genome: ERR864073_bin.141_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: USA

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

characteristic: organism

characteristic: sample derived from

text: Illumina HiSeq 1000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR864073_bin.20_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes version 3.10.139

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured euryarchaeote

characteristic: project name

text: uncultured euryarchaeote

text: Illumina HiSeq 1000

text: multi-marker approach

text: Metagenome-assembled genome: ERR864073_bin.44_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: USA

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: uncultured Rhodobiaceae bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Rhodobiaceae bacterium

text: Illumina HiSeq 1000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR864073_bin.60_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes version 3.10.139

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured Flavobacteriales bacterium

characteristic: project name

text: uncultured Flavobacteriales bacterium

text: Illumina HiSeq 1000

text: multi-marker approach

text: Metagenome-assembled genome: ERR864073_bin.66_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Multiple fragments where gaps span repetitive regions. Presence of the 23S, 16S, and 5S rRNA genes and at least 18 tRNAs

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: USA

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: uncultured Bacteroidetes bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Bacteroidetes bacterium

text: Illumina HiSeq 1000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR864073_bin.76_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes version 3.10.139

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured Flavobacteriaceae bacterium

characteristic: project name

text: uncultured Flavobacteriaceae bacterium

text: Illumina HiSeq 1000

text: multi-marker approach

text: Metagenome-assembled genome: ERR864073_bin.77_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR864075 of study ERP010213.

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: USA

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: uncultured euryarchaeote

characteristic: organism

characteristic: sample derived from

text: uncultured euryarchaeote

text: Illumina HiSeq 1000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR864075_bin.102_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes version 3.10.139

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: Thermus thermophilus

characteristic: project name

text: Thermus thermophilus

text: Illumina HiSeq 1000

text: multi-marker approach

text: Metagenome-assembled genome: ERR864075_bin.129_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: USA

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: uncultured Verrucomicrobiales bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Verrucomicrobiales bacterium

text: Illumina HiSeq 1000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR864075_bin.13_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes version 3.10.139

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured Phycisphaerae bacterium

characteristic: project name

text: uncultured Phycisphaerae bacterium

text: Illumina HiSeq 1000

text: multi-marker approach

text: Metagenome-assembled genome: ERR864075_bin.163_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: USA

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: uncultured euryarchaeote

characteristic: organism

characteristic: sample derived from

text: uncultured euryarchaeote

text: Illumina HiSeq 1000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR864075_bin.166_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes version 3.10.139

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured Planctomycetaceae bacterium

text: Microbial communities mediate the biogeochemical cycles that drive ecosystems, and it is important to understand how these communities are affected by changing environmental conditions, especially in complex coastal zones. As fresh and marine waters mix in estuaries and river plumes, the salinity, temperature, and nutrient gradients that are generated strongly influence bacterioplankton community structure, yet, a parallel shift in functional diversity has not been described. Metagenomic and metatranscriptomic analyses were conducted on five water samples spanning the salinity gradient of the Columbia River coastal margin, including river, estuary, plume, and ocean, in August 2010. Samples were pre-filtered through 3 μm filters and collected on 0.2 μm filters, thus results were focused on changes among free-living microbial communities. Results from metagenomic 16S rRNA sequences showed taxonomically distinct bacterial communities in river, estuary, and coastal ocean. Despite the strong salinity gradient observed over sampling locations (0 to 33), the functional gene profiles in the metagenomes were very similar from river to ocean with an average similarity of 82%. The metatranscriptomes, however, were an average of only 31% similar. We observed shifts from river to ocean in the abundance of genes encoding specific functions, such as catabolic pathways, osmoregulators, and metal transporters. Additionally, genes specifying both bacterial oxygenic and anoxygenic photosynthesis were highly abundant and expressed in the estuary and plume. Denitrification genes were found throughout the Columbia River coastal margin, and most highly expressed in the estuary. Across a river to ocean gradient, the free-living microbial community followed three different patterns of diversity: 1) the taxonomy of the community shifted strongly with salinity, 2) metabolic potential was highly similar across samples, with only small differences in functional gene abundance from river to ocean, and 3) gene expression was highly variable and generally was independent of changes in salinity.

characteristic: project name

text: uncultured Planctomycetaceae bacterium

text: Illumina HiSeq 1000

text: multi-marker approach

text: Metagenome-assembled genome: ERR864075_bin.167_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: USA

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: uncultured bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured bacterium

text: Illumina HiSeq 1000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR864075_bin.172_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes version 3.10.139

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured Chromatiales bacterium

characteristic: project name

text: uncultured Chromatiales bacterium

text: Illumina HiSeq 1000

text: multi-marker approach

text: Metagenome-assembled genome: ERR864075_bin.37_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: USA

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: uncultured Flavobacteriales bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Flavobacteriales bacterium

text: Illumina HiSeq 1000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR864075_bin.8_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes version 3.10.139

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR864077 of study ERP010213.

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured Alphaproteobacteria bacterium

characteristic: project name

text: Illumina HiSeq 1000

text: multi-marker approach

text: Metagenome-assembled genome: ERR864077_bin.114_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: USA

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: uncultured Micrococcales bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Micrococcales bacterium

text: Illumina HiSeq 1000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR864077_bin.127_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes version 3.10.139

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured Bacteroidetes bacterium

characteristic: project name

text: uncultured Bacteroidetes bacterium

text: Illumina HiSeq 1000

text: multi-marker approach

text: Metagenome-assembled genome: ERR864077_bin.138_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: USA

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: uncultured Burkholderiales bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Burkholderiales bacterium

text: Illumina HiSeq 1000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR864077_bin.40_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes version 3.10.139

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured Sphingobacteriaceae bacterium

characteristic: project name

text: Illumina HiSeq 1000

text: multi-marker approach

text: Metagenome-assembled genome: ERR864077_bin.88_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: MegaHit

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR868378 of study ERP003628.

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: not provided

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: uncultured Myxococcales bacterium

characteristic: organism

text: Seawater was filtered from different depths to retain small and large cell sizes. The DNA was extracted and submitted to high throughput sequencing.

characteristic: sample derived from

text: uncultured Myxococcales bacterium

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR868378_bin.19_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR868484 of study ERP003628.

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: not provided

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: project name

text: uncultured Clostridia bacterium

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: ERR868484_bin.32_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR1819825 of study SRP055532.

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: Australia

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: uncultured euryarchaeote

characteristic: organism

text: Metagenomic survey of the microbial communities in the Great Barrier Reef lagoon in 2009 and 2010

characteristic: sample derived from

text: uncultured euryarchaeote

text: Illumina Genome Analyzer II

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR1819825_bin.15_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured Rhodobacteraceae bacterium

characteristic: project name

text: uncultured Rhodobacteraceae bacterium

text: Illumina Genome Analyzer II

text: multi-marker approach

text: Metagenome-assembled genome: SRR1819825_bin.51_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: Australia

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: uncultured euryarchaeote

characteristic: organism

characteristic: sample derived from

text: uncultured euryarchaeote

text: Illumina Genome Analyzer II

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR1819825_bin.54_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.14.1

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR3401481 of study SRP073479.

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: Pacific Ocean

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured Flammeovirgaceae bacterium

text: Marine plastic debris has become a significant concern in ocean ecosystems worldwide. Little is known however, about its influence on microbial community structure and function. In 2008, we surveyed microbial communities and metabolic activities in seawater and on plastic on an oceanographic expedition through the ‘Great Pacific garbage patch’. The concentration of plastic particles in surface seawater within different size classes (2-5 mm and > 5 mm) ranged from 0.35-3.7 particles m-3 across sampling stations. These densities and the particle size distribution were consistent with previous values reported in the North Pacific Ocean. Net community oxygen production (NCP = gross primary production - community respiration) on plastic debris was positive and so net autotrophic, whereas NCP in bulk seawater was close to zero. Scanning electron microscopy and metagenomic sequencing of plastic-attached communities revealed the dominance of a few metazoan taxa, and a diverse assemblage of photoautotrophic and heterotrophic protists and bacteria. Bryozoa, Cyanobacteria, Alphaproteobacteria and Bacteroidetes dominated all plastic particles, regardless of particle size. Bacteria inhabiting plastic were taxonomically distinct from surrounding picoplankton, and appeared well adapted to a surface-associated lifestyle. Genes with significantly higher abundances among plastic-attached bacteria included che genes, secretion system genes and nifH genes, suggesting enrichment for chemotaxis, frequent cell-to-cell interactions and nitrogen fixation. In aggregate, our findings suggest that plastic debris forms a habitat for complex microbial assemblages that have lifestyles, metabolic pathways and biogeochemical activities that are distinct from free-living planktonic microbial communities.

characteristic: project name

text: uncultured Flammeovirgaceae bacterium

text: NextSeq 500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3401481_bin.23_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaSPAdes v3.14.1

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: Pacific Ocean

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: uncultured Rhodobacteraceae bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Rhodobacteraceae bacterium

text: NextSeq 500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3401481_bin.56_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.14.1

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: Pacific Ocean

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured Halomicronema sp.

characteristic: project name

text: uncultured Halomicronema sp.

text: NextSeq 500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3401481_bin.65_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.14.1

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: Pacific Ocean

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: uncultured Flammeovirgaceae bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Flammeovirgaceae bacterium

text: NextSeq 500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3401481_bin.67_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.14.1

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: Pacific Ocean

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured Phycisphaerales bacterium

characteristic: project name

text: uncultured Phycisphaerales bacterium

text: NextSeq 500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3401481_bin.71_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.14.1

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR3401482 of study SRP073479.

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: Pacific Ocean

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: uncultured cyanobacterium

characteristic: organism

characteristic: sample derived from

text: uncultured cyanobacterium

text: NextSeq 500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3401482_bin.11_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.14.1

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: Pacific Ocean

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured Labrenzia sp.

characteristic: project name

text: uncultured Labrenzia sp.

text: NextSeq 500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3401482_bin.53_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.14.1

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: Pacific Ocean

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: uncultured Parvularcula sp.

characteristic: organism

characteristic: sample derived from

text: uncultured Parvularcula sp.

text: NextSeq 500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3401482_bin.62_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.14.1

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: Pacific Ocean

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured Sphingomonadales bacterium

characteristic: project name

text: uncultured Sphingomonadales bacterium

text: NextSeq 500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3401482_bin.73_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.14.1

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: Pacific Ocean

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: uncultured Shimia sp.

characteristic: organism

characteristic: sample derived from

text: uncultured Shimia sp.

text: NextSeq 500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3401482_bin.76_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.14.1

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR3401483 of study SRP073479.

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: Pacific Ocean

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured Ilumatobacter sp.

characteristic: project name

text: uncultured Ilumatobacter sp.

text: NextSeq 500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3401483_bin.19_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.14.1

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: Pacific Ocean

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: uncultured Chlamydiae bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Chlamydiae bacterium

text: NextSeq 500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3401483_bin.28_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.14.1

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: Pacific Ocean

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured Neomegalonema sp.

characteristic: project name

text: uncultured Neomegalonema sp.

text: NextSeq 500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3401483_bin.43_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.14.1

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: Pacific Ocean

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: uncultured Calditrichaeota bacterium

characteristic: organism

text: Marine plastic debris has become a significant concern in ocean ecosystems worldwide. Little is known however, about its influence on microbial community structure and function. In 2008, we surveyed microbial communities and metabolic activities in seawater and on plastic on an oceanographic expedition through the ‘Great Pacific garbage patch’. The concentration of plastic particles in surface seawater within different size classes (2-5 mm and > 5 mm) ranged from 0.35-3.7 particles m-3 across sampling stations. These densities and the particle size distribution were consistent with previous values reported in the North Pacific Ocean. Net community oxygen production (NCP = gross primary production - community respiration) on plastic debris was positive and so net autotrophic, whereas NCP in bulk seawater was close to zero. Scanning electron microscopy and metagenomic sequencing of plastic-attached communities revealed the dominance of a few metazoan taxa, and a diverse assemblage of photoautotrophic and heterotrophic protists and bacteria. Bryozoa, Cyanobacteria, Alphaproteobacteria and Bacteroidetes dominated all plastic particles, regardless of particle size. Bacteria inhabiting plastic were taxonomically distinct from surrounding picoplankton, and appeared well adapted to a surface-associated lifestyle. Genes with significantly higher abundances among plastic-attached bacteria included che genes, secretion system genes and nifH genes, suggesting enrichment for chemotaxis, frequent cell-to-cell interactions and nitrogen fixation. In aggregate, our findings suggest that plastic debris forms a habitat for complex microbial assemblages that have lifestyles, metabolic pathways and biogeochemical activities that are distinct from free-living planktonic microbial communities.

characteristic: sample derived from

text: uncultured Calditrichaeota bacterium

text: NextSeq 500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3401483_bin.45_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes v3.14.1

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: Pacific Ocean

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured Flammeovirgaceae bacterium

characteristic: project name

text: uncultured Flammeovirgaceae bacterium

text: NextSeq 500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3401483_bin.57_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.14.1

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: Pacific Ocean

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: uncultured Spirochaetia bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Spirochaetia bacterium

text: NextSeq 500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3401483_bin.59_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.14.1

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: Pacific Ocean

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured Lewinella sp.

characteristic: project name

text: uncultured Lewinella sp.

text: NextSeq 500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3401483_bin.64_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.14.1

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: Pacific Ocean

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: uncultured Muricauda sp.

characteristic: organism

characteristic: sample derived from

text: uncultured Muricauda sp.

text: NextSeq 500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3401483_bin.85_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.14.1

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: Pacific Ocean

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured Pleurocapsa sp.

characteristic: project name

text: uncultured Pleurocapsa sp.

text: NextSeq 500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3401483_bin.89_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.14.1

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: Pacific Ocean

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: uncultured Myxococcales bacterium

characteristic: organism

characteristic: sample derived from

text: uncultured Myxococcales bacterium

text: NextSeq 500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3401483_bin.95_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.14.1

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR3401485 of study SRP073479.

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: Pacific Ocean

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured Flavobacteriales bacterium

characteristic: project name

text: uncultured Flavobacteriales bacterium

text: NextSeq 500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3401485_bin.10_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.14.1

characteristic: assembly software

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Environmental

text: Aquatic

text: Marine

text: Pacific Ocean

text: metagenome-assembled genome

text: marine metagenome

text: marine metagenome

text: uncultured Roseivirga sp.

characteristic: organism

characteristic: sample derived from

text: uncultured Roseivirga sp.

text: NextSeq 500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3401485_bin.38_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes v3.14.1

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Aquatic

characteristic: environment (feature)

text: Marine

characteristic: environment (material)

text: Pacific Ocean

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: marine metagenome

text: marine metagenome

characteristic: metagenomic source

text: uncultured cyanobacterium

characteristic: project name

text: uncultured cyanobacterium

text: NextSeq 500

text: multi-marker approach

text: Metagenome-assembled genome: SRR3401485_bin.73_CONCOCT_v1.1_MAG