characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: not provided

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Alistipes sp.

characteristic: organism

characteristic: project name

text: uncultured Alistipes sp.

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: DRR014734_bin.23_CONCOCT_v1.1_MAG

characteristic: title


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: not provided

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run DRR014734 of study DRP001303.

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

unit: DD

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Lachnospiraceae bacterium

text: Polyamines (PAs), such as putrescine, Spermidine, and spermine are related with cvarious bioactivity include strong anti-inflammatory function. Increasing PA concentration in intestine using probiotics; Bifidobacterium animalis subsp. lactis LKM512 caused inhibition of senescence and enhancing longevity of mice. We found that Arg intake also showed increase PAs concentration especially Putrescine. Mouse intakes Arg and LKM 512 resulted inhibited systemic inflammation, prolonged longevity, promoted spatial learning and memory. Metagenome analysis of intestinal mictobiota in those mice showed that detection of PA synthesis-related gene were different significantly between control and test mice.

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: DRR014734_bin.25_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: not provided

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Tenericutes bacterium

characteristic: organism

characteristic: project name

text: uncultured Tenericutes bacterium

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: DRR014734_bin.4_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: not provided

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Clostridia bacterium

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: DRR014734_bin.50_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: not provided

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: organism

characteristic: project name

text: uncultured Clostridia bacterium

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: DRR014734_bin.68_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: not provided

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Parabacteroides sp.

characteristic: sample derived from

text: uncultured Parabacteroides sp.

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: DRR014734_bin.96_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: not provided

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run DRR014783 of study DRP001306.

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: organism

characteristic: project name

text: uncultured Clostridia bacterium

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: DRR014783_bin.10_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: not provided

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Butyrivibrio sp.

characteristic: sample derived from

text: uncultured Butyrivibrio sp.

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: DRR014783_bin.131_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: not provided

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: Candidatus Homeothermus arabinoxylanisolvens

characteristic: organism

characteristic: project name

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: DRR014783_bin.20_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: not provided

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: DRR014783_bin.22_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: not provided

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Bacillales bacterium

characteristic: organism

characteristic: project name

text: uncultured Bacillales bacterium

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: DRR014783_bin.34_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: not provided

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Prevotella sp.

characteristic: sample derived from

text: uncultured Prevotella sp.

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: DRR014783_bin.61_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: not provided

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: organism

characteristic: project name

text: uncultured Clostridia bacterium

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: DRR014783_bin.64_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: not provided

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Porphyromonadaceae bacterium

characteristic: sample derived from

text: uncultured Porphyromonadaceae bacterium

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: DRR014783_bin.70_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: not provided

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Porphyromonadaceae bacterium

characteristic: organism

text: Polyamines (PAs), such as putrescine, Spermidine, and spermine are related with cvarious bioactivity include strong anti-inflammatory function. Increasing PA concentration in intestine using probiotics; Bifidobacterium animalis subsp. lactis LKM512 caused inhibition of senescence and enhancing longevity of mice. We found that Arg intake also showed increase PAs concentration especially Putrescine. Mouse intakes Arg and LKM 512 resulted inhibited systemic inflammation, prolonged longevity, promoted spatial learning and memory. Metagenome analysis of intestinal mictobiota in those mice showed that detection of PA synthesis-related gene were different significantly between control and test mice.

characteristic: project name

text: uncultured Porphyromonadaceae bacterium

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: DRR014783_bin.80_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: not provided

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run DRR014784 of study DRP001307.

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Clostridia bacterium

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: DRR014784_bin.101_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: not provided

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Lachnospiraceae bacterium

characteristic: organism

characteristic: project name

text: uncultured Lachnospiraceae bacterium

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: DRR014784_bin.129_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: not provided

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Turicibacter sp.

characteristic: sample derived from

text: uncultured Turicibacter sp.

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: DRR014784_bin.131_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: not provided

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Lachnospiraceae bacterium

characteristic: organism

characteristic: project name

text: uncultured Lachnospiraceae bacterium

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: DRR014784_bin.138_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: not provided

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Porphyromonadaceae bacterium

characteristic: sample derived from

text: uncultured Porphyromonadaceae bacterium

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: DRR014784_bin.144_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: not provided

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Lachnospiraceae bacterium

characteristic: organism

characteristic: project name

text: uncultured Lachnospiraceae bacterium

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: DRR014784_bin.160_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: not provided

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Clostridia bacterium

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: DRR014784_bin.19_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: not provided

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: organism

characteristic: project name

text: uncultured Clostridia bacterium

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: DRR014784_bin.29_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: not provided

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: DRR014784_bin.33_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: not provided

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: organism

characteristic: project name

text: uncultured Clostridia bacterium

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: DRR014784_bin.62_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: not provided

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: DRR014784_bin.65_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: not provided

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Porphyromonadaceae bacterium

characteristic: organism

characteristic: project name

text: uncultured Porphyromonadaceae bacterium

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: DRR014784_bin.69_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: not provided

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured bacterium

characteristic: sample derived from

text: uncultured bacterium

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: DRR014784_bin.70_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: not provided

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text: Polyamines (PAs), such as putrescine, Spermidine, and spermine are related with cvarious bioactivity include strong anti-inflammatory function. Increasing PA concentration in intestine using probiotics; Bifidobacterium animalis subsp. lactis LKM512 caused inhibition of senescence and enhancing longevity of mice. We found that Arg intake also showed increase PAs concentration especially Putrescine. Mouse intakes Arg and LKM 512 resulted inhibited systemic inflammation, prolonged longevity, promoted spatial learning and memory. Metagenome analysis of intestinal mictobiota in those mice showed that detection of PA synthesis-related gene were different significantly between control and test mice.

characteristic: project name

text: uncultured Lachnospiraceae bacterium

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: DRR014784_bin.78_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: not provided

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run DRR014784 of study DRP001307.

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: DRR014784_bin.92_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1135178 of study ERP013165.

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: France

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Veillonellaceae bacterium

characteristic: organism

text: Pig is a main species for livestock and biomedicine. The pig genome sequence was recently reported. To boost research, we established a catalogue of the genes of the gut microbiome based on faecal samples of 287 pigs from France, Denmark and China. More than 7.6 million non-redundant genes representing 719 metagenomic species were identified by deep metagenome sequencing, highlighting more similarities with the human than with the mouse catalogue. The pig and human catalogues share only 12.6 and 9.3 % of their genes, respectively, but 70 and 95% of their functional pathways. The pig gut microbiota is influenced by gender, age and breed. Analysis of the prevalence of antibiotics resistance genes (ARGs) reflected antibiotics supplementation in each farm system, and revealed that non-antibiotics-fed animals still harbour ARGs. The pig catalogue creates a resource for whole metagenomics-based studies, highly valuable for research in biomedicine and for sustainable knowledge-based pig farming.

characteristic: project name

text: uncultured Veillonellaceae bacterium

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: ERR1135178_bin.0_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes version 3.10.139

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: France

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Parabacteroides sp.

characteristic: sample derived from

text: uncultured Parabacteroides sp.

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1135178_bin.104_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: France

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Bacteroidales bacterium

characteristic: organism

characteristic: project name

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: ERR1135178_bin.112_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes version 3.10.139

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: France

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Prevotella sp.

characteristic: sample derived from

text: uncultured Prevotella sp.

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1135178_bin.122_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: France

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: Streptococcus hyointestinalis

characteristic: organism

characteristic: project name

text: Streptococcus hyointestinalis

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: ERR1135178_bin.141_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes version 3.10.139

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: France

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Bacteroidales bacterium

characteristic: sample derived from

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1135178_bin.158_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: France

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Bacteroidales bacterium

characteristic: organism

characteristic: project name

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: ERR1135178_bin.49_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes version 3.10.139

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: France

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Clostridia bacterium

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1135178_bin.53_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: France

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Campylobacter sp.

characteristic: organism

characteristic: project name

text: uncultured Campylobacter sp.

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: ERR1135178_bin.57_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes version 3.10.139

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: France

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Ruminococcus sp.

characteristic: sample derived from

text: uncultured Ruminococcus sp.

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1135178_bin.82_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: France

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Erysipelotrichaceae bacterium

characteristic: organism

characteristic: project name

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: ERR1135178_bin.89_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1278072 of study ERP014306.

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Bacteroidales bacterium

text: The moose (Alces alces) manages to utilize energy from fiber-rich lignocellulose material through enzymes produced by the microbes in its rumen. Some of these specialized enzymes may outperform cellulases and hemicellulases that are used in bio-refinery processes today. Shotgun metagenomics offers a method to study microbial communities in detail and to capture the gene sequences coding for enzymes that can be produced in the laboratory. We applied the technique on rumen material from moose. Through a binning approach, a large number of metagenome-assembled genomes (MAGs) from single species and strains were isolated and characterized based on taxonomy and carbohydrate active enzyme (CAZY) profile. Most of the 99 MAGs were classified to the bacterial phyla Bacteriodetes and Firmicutes, which is in agreement with previous studies of rumen from moose and other herbivores. Phylogenomic analysis of MAGs revealed novel clades within Bacteriodetes with no related previously characterized genome. In addition, new genomes that were closely related to the recently described Melainabacteria, newly proposed candidate divisions SR1 and TM7 and previously uncharacterized Alphaproteobacteria were also retrieved. Putative cellulolytic functions were observed in several clades of Bacteroidetes and for MAGs of genus Ruminococcus, Fibrobacteres, and Treponema. Cellulosome components were present in MAGs of Ruminococcus but also in certain clades of Bacteroidetes. Cellulosomes are generally described in the Firmicutes family Clostridiales. Multiple putative cellulases were identified within species from several phyla. At a general level, the most frequently occurring polysaccharide-degrading enzymes targeted hemicellulose, starch and pectin. This study describes the first shotgun-metagenomic characterization of moose rumen and the high number of reconstructed genomes provides a valuable link between phylogeny and cellulolytic role.

characteristic: sample derived from

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278072_bin.1_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Fretibacterium sp.

characteristic: organism

characteristic: project name

text: uncultured Fretibacterium sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278072_bin.130_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Bacillales bacterium

characteristic: sample derived from

text: uncultured Bacillales bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278072_bin.141_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Selenomonadales bacterium

characteristic: organism

characteristic: project name

text: uncultured Selenomonadales bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278072_bin.153_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Fretibacterium sp.

characteristic: sample derived from

text: uncultured Fretibacterium sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278072_bin.155_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Prevotella sp.

characteristic: organism

characteristic: project name

text: uncultured Prevotella sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278072_bin.159_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Ruminobacter sp.

characteristic: sample derived from

text: uncultured Ruminobacter sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278072_bin.16_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Bacteroidales bacterium

characteristic: organism

characteristic: project name

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278072_bin.161_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured bacterium

characteristic: sample derived from

text: uncultured bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278072_bin.18_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Lachnospiraceae bacterium

characteristic: organism

characteristic: project name

text: uncultured Lachnospiraceae bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278072_bin.2_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Bacteroidales bacterium

characteristic: sample derived from

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278072_bin.48_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Oxalobacter sp.

characteristic: organism

characteristic: project name

text: uncultured Oxalobacter sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278072_bin.5_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Ruminococcus sp.

characteristic: sample derived from

text: uncultured Ruminococcus sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278072_bin.68_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Elusimicrobia bacterium

characteristic: organism

characteristic: project name

text: uncultured Elusimicrobia bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278072_bin.71_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1278072 of study ERP014306.

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Fretibacterium sp.

characteristic: sample derived from

text: uncultured Fretibacterium sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278072_bin.79_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Bacteroidales bacterium

characteristic: organism

text: The moose (Alces alces) manages to utilize energy from fiber-rich lignocellulose material through enzymes produced by the microbes in its rumen. Some of these specialized enzymes may outperform cellulases and hemicellulases that are used in bio-refinery processes today. Shotgun metagenomics offers a method to study microbial communities in detail and to capture the gene sequences coding for enzymes that can be produced in the laboratory. We applied the technique on rumen material from moose. Through a binning approach, a large number of metagenome-assembled genomes (MAGs) from single species and strains were isolated and characterized based on taxonomy and carbohydrate active enzyme (CAZY) profile. Most of the 99 MAGs were classified to the bacterial phyla Bacteriodetes and Firmicutes, which is in agreement with previous studies of rumen from moose and other herbivores. Phylogenomic analysis of MAGs revealed novel clades within Bacteriodetes with no related previously characterized genome. In addition, new genomes that were closely related to the recently described Melainabacteria, newly proposed candidate divisions SR1 and TM7 and previously uncharacterized Alphaproteobacteria were also retrieved. Putative cellulolytic functions were observed in several clades of Bacteroidetes and for MAGs of genus Ruminococcus, Fibrobacteres, and Treponema. Cellulosome components were present in MAGs of Ruminococcus but also in certain clades of Bacteroidetes. Cellulosomes are generally described in the Firmicutes family Clostridiales. Multiple putative cellulases were identified within species from several phyla. At a general level, the most frequently occurring polysaccharide-degrading enzymes targeted hemicellulose, starch and pectin. This study describes the first shotgun-metagenomic characterization of moose rumen and the high number of reconstructed genomes provides a valuable link between phylogeny and cellulolytic role.

characteristic: project name

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278072_bin.9_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1278073 of study ERP014306.

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Selenomonadales bacterium

characteristic: sample derived from

text: uncultured Selenomonadales bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278073_bin.106_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Bacteroidales bacterium

characteristic: organism

characteristic: project name

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278073_bin.115_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Methanobrevibacter sp.

characteristic: sample derived from

text: uncultured Methanobrevibacter sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278073_bin.120_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Bacteroidales bacterium

characteristic: organism

characteristic: project name

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278073_bin.13_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Bacteroidales bacterium

characteristic: sample derived from

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278073_bin.19_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Succiniclasticum sp.

characteristic: organism

characteristic: project name

text: uncultured Succiniclasticum sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278073_bin.30_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured bacterium

characteristic: sample derived from

text: uncultured bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278073_bin.4_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Prevotella sp.

characteristic: organism

characteristic: project name

text: uncultured Prevotella sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278073_bin.50_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Prevotellaceae bacterium

characteristic: sample derived from

text: uncultured Prevotellaceae bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278073_bin.54_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Bacteroidales bacterium

characteristic: organism

characteristic: project name

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278073_bin.58_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1278074 of study ERP014306.

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Clostridia bacterium

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278074_bin.0_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Lachnospiraceae bacterium

characteristic: organism

characteristic: project name

text: uncultured Lachnospiraceae bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278074_bin.115_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Bacteroidetes bacterium

text: The moose (Alces alces) manages to utilize energy from fiber-rich lignocellulose material through enzymes produced by the microbes in its rumen. Some of these specialized enzymes may outperform cellulases and hemicellulases that are used in bio-refinery processes today. Shotgun metagenomics offers a method to study microbial communities in detail and to capture the gene sequences coding for enzymes that can be produced in the laboratory. We applied the technique on rumen material from moose. Through a binning approach, a large number of metagenome-assembled genomes (MAGs) from single species and strains were isolated and characterized based on taxonomy and carbohydrate active enzyme (CAZY) profile. Most of the 99 MAGs were classified to the bacterial phyla Bacteriodetes and Firmicutes, which is in agreement with previous studies of rumen from moose and other herbivores. Phylogenomic analysis of MAGs revealed novel clades within Bacteriodetes with no related previously characterized genome. In addition, new genomes that were closely related to the recently described Melainabacteria, newly proposed candidate divisions SR1 and TM7 and previously uncharacterized Alphaproteobacteria were also retrieved. Putative cellulolytic functions were observed in several clades of Bacteroidetes and for MAGs of genus Ruminococcus, Fibrobacteres, and Treponema. Cellulosome components were present in MAGs of Ruminococcus but also in certain clades of Bacteroidetes. Cellulosomes are generally described in the Firmicutes family Clostridiales. Multiple putative cellulases were identified within species from several phyla. At a general level, the most frequently occurring polysaccharide-degrading enzymes targeted hemicellulose, starch and pectin. This study describes the first shotgun-metagenomic characterization of moose rumen and the high number of reconstructed genomes provides a valuable link between phylogeny and cellulolytic role.

characteristic: sample derived from

text: uncultured Bacteroidetes bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278074_bin.127_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: organism

characteristic: project name

text: uncultured Clostridia bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278074_bin.128_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Prevotellaceae bacterium

characteristic: sample derived from

text: uncultured Prevotellaceae bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278074_bin.151_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Lachnospiraceae bacterium

characteristic: organism

characteristic: project name

text: uncultured Lachnospiraceae bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278074_bin.24_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Methanomicrobiales archaeon

characteristic: sample derived from

text: uncultured Methanomicrobiales archaeon

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278074_bin.27_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Prevotellaceae bacterium

characteristic: organism

characteristic: project name

text: uncultured Prevotellaceae bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278074_bin.33_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Bacteroidales bacterium

characteristic: sample derived from

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278074_bin.44_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Selenomonadales bacterium

characteristic: organism

characteristic: project name

text: uncultured Selenomonadales bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278074_bin.57_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Bacteroidales bacterium

characteristic: sample derived from

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278074_bin.74_CONCOCT_v1.1_MAG