characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Prevotellaceae bacterium

characteristic: organism

characteristic: project name

text: uncultured Prevotellaceae bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278074_bin.77_CONCOCT_v1.1_MAG

characteristic: title


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1278074 of study ERP014306.

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Prevotellaceae bacterium

text: The moose (Alces alces) manages to utilize energy from fiber-rich lignocellulose material through enzymes produced by the microbes in its rumen. Some of these specialized enzymes may outperform cellulases and hemicellulases that are used in bio-refinery processes today. Shotgun metagenomics offers a method to study microbial communities in detail and to capture the gene sequences coding for enzymes that can be produced in the laboratory. We applied the technique on rumen material from moose. Through a binning approach, a large number of metagenome-assembled genomes (MAGs) from single species and strains were isolated and characterized based on taxonomy and carbohydrate active enzyme (CAZY) profile. Most of the 99 MAGs were classified to the bacterial phyla Bacteriodetes and Firmicutes, which is in agreement with previous studies of rumen from moose and other herbivores. Phylogenomic analysis of MAGs revealed novel clades within Bacteriodetes with no related previously characterized genome. In addition, new genomes that were closely related to the recently described Melainabacteria, newly proposed candidate divisions SR1 and TM7 and previously uncharacterized Alphaproteobacteria were also retrieved. Putative cellulolytic functions were observed in several clades of Bacteroidetes and for MAGs of genus Ruminococcus, Fibrobacteres, and Treponema. Cellulosome components were present in MAGs of Ruminococcus but also in certain clades of Bacteroidetes. Cellulosomes are generally described in the Firmicutes family Clostridiales. Multiple putative cellulases were identified within species from several phyla. At a general level, the most frequently occurring polysaccharide-degrading enzymes targeted hemicellulose, starch and pectin. This study describes the first shotgun-metagenomic characterization of moose rumen and the high number of reconstructed genomes provides a valuable link between phylogeny and cellulolytic role.

characteristic: sample derived from

text: uncultured Prevotellaceae bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278074_bin.97_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1278103 of study ERP014306.

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Lachnospiraceae bacterium

characteristic: organism

characteristic: project name

text: uncultured Lachnospiraceae bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278103_bin.107_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278103_bin.114_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: organism

characteristic: project name

text: uncultured Clostridia bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278103_bin.119_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Prevotellaceae bacterium

characteristic: sample derived from

text: uncultured Prevotellaceae bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278103_bin.128_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Bacteroidales bacterium

characteristic: organism

characteristic: project name

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278103_bin.131_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Fretibacterium sp.

characteristic: sample derived from

text: uncultured Fretibacterium sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278103_bin.14_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Candidatus Saccharibacteria bacterium

characteristic: organism

characteristic: project name

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278103_bin.189_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Prevotellaceae bacterium

characteristic: sample derived from

text: uncultured Prevotellaceae bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278103_bin.191_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Selenomonas sp.

characteristic: organism

characteristic: project name

text: uncultured Selenomonas sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278103_bin.195_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Clostridia bacterium

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278103_bin.198_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Methanobrevibacter sp.

characteristic: organism

characteristic: project name

text: uncultured Methanobrevibacter sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278103_bin.200_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Selenomonadales bacterium

characteristic: sample derived from

text: uncultured Selenomonadales bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278103_bin.24_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Treponema sp.

characteristic: organism

text: The moose (Alces alces) manages to utilize energy from fiber-rich lignocellulose material through enzymes produced by the microbes in its rumen. Some of these specialized enzymes may outperform cellulases and hemicellulases that are used in bio-refinery processes today. Shotgun metagenomics offers a method to study microbial communities in detail and to capture the gene sequences coding for enzymes that can be produced in the laboratory. We applied the technique on rumen material from moose. Through a binning approach, a large number of metagenome-assembled genomes (MAGs) from single species and strains were isolated and characterized based on taxonomy and carbohydrate active enzyme (CAZY) profile. Most of the 99 MAGs were classified to the bacterial phyla Bacteriodetes and Firmicutes, which is in agreement with previous studies of rumen from moose and other herbivores. Phylogenomic analysis of MAGs revealed novel clades within Bacteriodetes with no related previously characterized genome. In addition, new genomes that were closely related to the recently described Melainabacteria, newly proposed candidate divisions SR1 and TM7 and previously uncharacterized Alphaproteobacteria were also retrieved. Putative cellulolytic functions were observed in several clades of Bacteroidetes and for MAGs of genus Ruminococcus, Fibrobacteres, and Treponema. Cellulosome components were present in MAGs of Ruminococcus but also in certain clades of Bacteroidetes. Cellulosomes are generally described in the Firmicutes family Clostridiales. Multiple putative cellulases were identified within species from several phyla. At a general level, the most frequently occurring polysaccharide-degrading enzymes targeted hemicellulose, starch and pectin. This study describes the first shotgun-metagenomic characterization of moose rumen and the high number of reconstructed genomes provides a valuable link between phylogeny and cellulolytic role.

characteristic: project name

text: uncultured Treponema sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278103_bin.26_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1278103 of study ERP014306.

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Clostridia bacterium

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278103_bin.37_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Treponema sp.

characteristic: organism

characteristic: project name

text: uncultured Treponema sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278103_bin.49_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Treponema sp.

characteristic: sample derived from

text: uncultured Treponema sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278103_bin.5_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Selenomonadales bacterium

characteristic: organism

characteristic: project name

text: uncultured Selenomonadales bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278103_bin.51_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Porphyromonadaceae bacterium

characteristic: sample derived from

text: uncultured Porphyromonadaceae bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278103_bin.68_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1278104 of study ERP014306.

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Fretibacterium sp.

characteristic: organism

characteristic: project name

text: uncultured Fretibacterium sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278104_bin.105_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Treponema sp.

characteristic: sample derived from

text: uncultured Treponema sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278104_bin.132_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Porphyromonadaceae bacterium

characteristic: organism

characteristic: project name

text: uncultured Porphyromonadaceae bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278104_bin.144_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Treponema sp.

characteristic: sample derived from

text: uncultured Treponema sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278104_bin.15_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Prevotellaceae bacterium

characteristic: organism

characteristic: project name

text: uncultured Prevotellaceae bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278104_bin.219_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Eubacterium sp.

characteristic: sample derived from

text: uncultured Eubacterium sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278104_bin.22_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Lachnospiraceae bacterium

characteristic: organism

characteristic: project name

text: uncultured Lachnospiraceae bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278104_bin.223_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Elusimicrobium sp.

text: The moose (Alces alces) manages to utilize energy from fiber-rich lignocellulose material through enzymes produced by the microbes in its rumen. Some of these specialized enzymes may outperform cellulases and hemicellulases that are used in bio-refinery processes today. Shotgun metagenomics offers a method to study microbial communities in detail and to capture the gene sequences coding for enzymes that can be produced in the laboratory. We applied the technique on rumen material from moose. Through a binning approach, a large number of metagenome-assembled genomes (MAGs) from single species and strains were isolated and characterized based on taxonomy and carbohydrate active enzyme (CAZY) profile. Most of the 99 MAGs were classified to the bacterial phyla Bacteriodetes and Firmicutes, which is in agreement with previous studies of rumen from moose and other herbivores. Phylogenomic analysis of MAGs revealed novel clades within Bacteriodetes with no related previously characterized genome. In addition, new genomes that were closely related to the recently described Melainabacteria, newly proposed candidate divisions SR1 and TM7 and previously uncharacterized Alphaproteobacteria were also retrieved. Putative cellulolytic functions were observed in several clades of Bacteroidetes and for MAGs of genus Ruminococcus, Fibrobacteres, and Treponema. Cellulosome components were present in MAGs of Ruminococcus but also in certain clades of Bacteroidetes. Cellulosomes are generally described in the Firmicutes family Clostridiales. Multiple putative cellulases were identified within species from several phyla. At a general level, the most frequently occurring polysaccharide-degrading enzymes targeted hemicellulose, starch and pectin. This study describes the first shotgun-metagenomic characterization of moose rumen and the high number of reconstructed genomes provides a valuable link between phylogeny and cellulolytic role.

characteristic: sample derived from

text: uncultured Elusimicrobium sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278104_bin.225_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Treponema sp.

characteristic: organism

characteristic: project name

text: uncultured Treponema sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278104_bin.228_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Bacteroidetes bacterium

characteristic: sample derived from

text: uncultured Bacteroidetes bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278104_bin.231_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Prevotella sp.

characteristic: organism

characteristic: project name

text: uncultured Prevotella sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278104_bin.238_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278104_bin.25_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Selenomonadales bacterium

characteristic: organism

characteristic: project name

text: uncultured Selenomonadales bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278104_bin.38_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Marinilabiliales bacterium

characteristic: sample derived from

text: uncultured Marinilabiliales bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278104_bin.43_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1278104 of study ERP014306.

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Ruminococcus sp.

characteristic: organism

characteristic: project name

text: uncultured Ruminococcus sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278104_bin.47_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Prevotellaceae bacterium

characteristic: sample derived from

text: uncultured Prevotellaceae bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278104_bin.57_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Treponema sp.

characteristic: organism

characteristic: project name

text: uncultured Treponema sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278104_bin.75_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Clostridia bacterium

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278104_bin.87_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Lachnospiraceae bacterium

characteristic: organism

characteristic: project name

text: uncultured Lachnospiraceae bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278104_bin.99_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1278105 of study ERP014306.

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Porphyromonadaceae bacterium

characteristic: sample derived from

text: uncultured Porphyromonadaceae bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278105_bin.101_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Ruminococcus sp.

characteristic: organism

text: The moose (Alces alces) manages to utilize energy from fiber-rich lignocellulose material through enzymes produced by the microbes in its rumen. Some of these specialized enzymes may outperform cellulases and hemicellulases that are used in bio-refinery processes today. Shotgun metagenomics offers a method to study microbial communities in detail and to capture the gene sequences coding for enzymes that can be produced in the laboratory. We applied the technique on rumen material from moose. Through a binning approach, a large number of metagenome-assembled genomes (MAGs) from single species and strains were isolated and characterized based on taxonomy and carbohydrate active enzyme (CAZY) profile. Most of the 99 MAGs were classified to the bacterial phyla Bacteriodetes and Firmicutes, which is in agreement with previous studies of rumen from moose and other herbivores. Phylogenomic analysis of MAGs revealed novel clades within Bacteriodetes with no related previously characterized genome. In addition, new genomes that were closely related to the recently described Melainabacteria, newly proposed candidate divisions SR1 and TM7 and previously uncharacterized Alphaproteobacteria were also retrieved. Putative cellulolytic functions were observed in several clades of Bacteroidetes and for MAGs of genus Ruminococcus, Fibrobacteres, and Treponema. Cellulosome components were present in MAGs of Ruminococcus but also in certain clades of Bacteroidetes. Cellulosomes are generally described in the Firmicutes family Clostridiales. Multiple putative cellulases were identified within species from several phyla. At a general level, the most frequently occurring polysaccharide-degrading enzymes targeted hemicellulose, starch and pectin. This study describes the first shotgun-metagenomic characterization of moose rumen and the high number of reconstructed genomes provides a valuable link between phylogeny and cellulolytic role.

characteristic: project name

text: uncultured Ruminococcus sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278105_bin.102_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278105_bin.117_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Prevotella sp.

characteristic: organism

characteristic: project name

text: uncultured Prevotella sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278105_bin.124_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Treponema sp.

characteristic: sample derived from

text: uncultured Treponema sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278105_bin.13_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Treponema sp.

characteristic: organism

characteristic: project name

text: uncultured Treponema sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278105_bin.134_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Selenomonadales bacterium

characteristic: sample derived from

text: uncultured Selenomonadales bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278105_bin.146_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Selenomonadales bacterium

characteristic: organism

characteristic: project name

text: uncultured Selenomonadales bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278105_bin.15_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Fretibacterium sp.

characteristic: sample derived from

text: uncultured Fretibacterium sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278105_bin.157_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Bacteroidales bacterium

characteristic: organism

characteristic: project name

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278105_bin.159_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Selenomonadales bacterium

characteristic: sample derived from

text: uncultured Selenomonadales bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278105_bin.186_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Treponema sp.

characteristic: organism

characteristic: project name

text: uncultured Treponema sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278105_bin.188_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Ruminococcus sp.

characteristic: sample derived from

text: uncultured Ruminococcus sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278105_bin.198_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Methanomicrobiales archaeon

characteristic: organism

characteristic: project name

text: uncultured Methanomicrobiales archaeon

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278105_bin.204_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Selenomonadales bacterium

characteristic: sample derived from

text: uncultured Selenomonadales bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278105_bin.27_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1278105 of study ERP014306.

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Treponema sp.

characteristic: organism

characteristic: project name

text: uncultured Treponema sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278105_bin.36_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Treponema sp.

text: The moose (Alces alces) manages to utilize energy from fiber-rich lignocellulose material through enzymes produced by the microbes in its rumen. Some of these specialized enzymes may outperform cellulases and hemicellulases that are used in bio-refinery processes today. Shotgun metagenomics offers a method to study microbial communities in detail and to capture the gene sequences coding for enzymes that can be produced in the laboratory. We applied the technique on rumen material from moose. Through a binning approach, a large number of metagenome-assembled genomes (MAGs) from single species and strains were isolated and characterized based on taxonomy and carbohydrate active enzyme (CAZY) profile. Most of the 99 MAGs were classified to the bacterial phyla Bacteriodetes and Firmicutes, which is in agreement with previous studies of rumen from moose and other herbivores. Phylogenomic analysis of MAGs revealed novel clades within Bacteriodetes with no related previously characterized genome. In addition, new genomes that were closely related to the recently described Melainabacteria, newly proposed candidate divisions SR1 and TM7 and previously uncharacterized Alphaproteobacteria were also retrieved. Putative cellulolytic functions were observed in several clades of Bacteroidetes and for MAGs of genus Ruminococcus, Fibrobacteres, and Treponema. Cellulosome components were present in MAGs of Ruminococcus but also in certain clades of Bacteroidetes. Cellulosomes are generally described in the Firmicutes family Clostridiales. Multiple putative cellulases were identified within species from several phyla. At a general level, the most frequently occurring polysaccharide-degrading enzymes targeted hemicellulose, starch and pectin. This study describes the first shotgun-metagenomic characterization of moose rumen and the high number of reconstructed genomes provides a valuable link between phylogeny and cellulolytic role.

characteristic: sample derived from

text: uncultured Treponema sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278105_bin.43_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Bacteroidales bacterium

characteristic: organism

characteristic: project name

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278105_bin.52_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Prevotellaceae bacterium

characteristic: sample derived from

text: uncultured Prevotellaceae bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278105_bin.54_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Lachnospiraceae bacterium

characteristic: organism

characteristic: project name

text: uncultured Lachnospiraceae bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278105_bin.55_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Clostridia bacterium

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278105_bin.56_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Gammaproteobacteria bacterium

characteristic: organism

characteristic: project name

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278105_bin.6_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Treponema sp.

characteristic: sample derived from

text: uncultured Treponema sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278105_bin.64_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Fretibacterium sp.

characteristic: organism

characteristic: project name

text: uncultured Fretibacterium sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278105_bin.71_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Fretibacterium sp.

characteristic: sample derived from

text: uncultured Fretibacterium sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278105_bin.73_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Bacteroidales bacterium

characteristic: organism

characteristic: project name

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278105_bin.80_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Methanomassiliicoccales archaeon

characteristic: sample derived from

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278105_bin.89_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: Sweden

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: organism

characteristic: project name

text: uncultured Clostridia bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1278105_bin.92_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1278105 of study ERP014306.

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Prevotellaceae bacterium

characteristic: sample derived from

text: uncultured Prevotellaceae bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1278105_bin.98_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: not provided

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1562573 of study ERP016797.

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Alistipes sp.

characteristic: organism

text: A total of 24 C57BL6 mice were used for this analysis. Six mice received dextran sodium sulfate (DSS) (3%) in their drinking water ab libitum. In addition to DSS treatment 6 mice were given sodium tungstate (0.2%) or sodium tungstate plus DSS in their drinking water ab libitum. A control group was included where 6 mice were given untreated water. All animals received their treatments for a total of 9 days.

characteristic: project name

text: uncultured Alistipes sp.

text: NextSeq 500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1562573_bin.1_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: not provided

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: NextSeq 500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1562573_bin.29_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: not provided

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Lachnospiraceae bacterium

characteristic: organism

characteristic: project name

text: uncultured Lachnospiraceae bacterium

text: NextSeq 500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1562573_bin.51_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: not provided

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Lachnoclostridium sp.

characteristic: sample derived from

text: uncultured Lachnoclostridium sp.

text: NextSeq 500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1562573_bin.52_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: not provided

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Lachnospiraceae bacterium

characteristic: organism

characteristic: project name

text: uncultured Lachnospiraceae bacterium

text: NextSeq 500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1562573_bin.9_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: not provided

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1562581 of study ERP016797.

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Clostridia bacterium

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: NextSeq 500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1562581_bin.13_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: not provided

text: CheckM

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: organism

characteristic: project name

text: uncultured Clostridia bacterium

text: NextSeq 500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1562581_bin.26_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: not provided

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: NextSeq 500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1562581_bin.41_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

text: not provided

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1562587 of study ERP016797.

characteristic: description

text: Host-associated

text: Mammals

text: Digestive system

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

text: gut metagenome

text: gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: organism

characteristic: project name

text: uncultured Clostridia bacterium

text: NextSeq 500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1562587_bin.2_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: Default

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: not provided

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Host-associated

characteristic: environment (biome)

text: Mammals

characteristic: environment (feature)

text: Digestive system

characteristic: environment (material)

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: gut metagenome

text: gut metagenome

text: uncultured Clostridia bacterium

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: NextSeq 500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1562587_bin.27_CONCOCT_v1.1_MAG