characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IRB Barcelona / BIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

characteristic: genotype

text: GFP+; CD45-

characteristic: immunophenotype

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

text: Mus musculus

text: OK

text: MKPPXAAX


status: PUBLIC

tag: attribute

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IRB Barcelona / BIST

characteristic: INSDC center name

characteristic: INSDC first public

text: public

tag: Namespace:IRB Barcelona / BIST

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: neoplastic cell

text: house mouse

characteristic: common name

text: Protocols: MTOs were established from primary tumors arising in genetically engineered mouse models bearing mutations in Apc, Kras, and p53 (AKP mouse model, Apc fl/fl, KrasG12D and Trp53 fl/fl). We analyzed by scRNAseq micro-dissected liver metastases generated by implantation of AKP MTOs in the caecum of c57BL/6 mice. The PGK is driven by a constitutive PGK promoter and therefore expressed in all tumor cells. GFP was used thus to separate tumor cells from the rest of healthy cells. For isolation of low numbers of tumour cells from mice without visible metastases, whole livers were thoroughly minced with razor blades. After an initial 30-minute digestion with 200 U/ml collagenase IV, samples were filtered through 100 μm meshes. Although most cells flowed through, a small fraction of the samples - highly enriched for tumour cells - was retained in the filter (Supplementary Figure 5c). Filters were then laid into a 6-well plate and covered with HBSS containing collagenase IV, Dispase II and DNase I. After re-digestion in a water bath for 30 minutes al 37 °C, most cells now seeped through the filters. Single-cell preparations were first blocked with anti-CD16/32 (clone 93; eBioscience) and then stained with APC anti-Epcam (118214; Biolegend) and BV605 anti-CD45 (103155, Biolegend) antibodies. Finally, cells were resuspended in HBSS with 0.5% FBS and DAPI (Sigma Aldrich). Freshly harvested GFP+ single cells were sorted into 96-well plates containing 2.5 µl of the Reaction buffer (1x Lysis Buffer, RNase Inhibitor 1U/µl). Full-length single-cell RNA-seq libraries were prepared using the SMART-Seq v5 Ultra Low Input RNA Kit for Sequencing (Takara Bio). All reactions were downscaled to one quarter of the original protocol and performed following thermal cycling manufacturer’s conditions.

characteristic: description

text: adult

characteristic: developmental stage

text: metastatic colorectal cancer

characteristic: disease

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

characteristic: organism

text: Mus musculus

text: liver micrometastasis

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Mus musculus

text: OK

characteristic: single cell well quality

characteristic: strain

text: FJKAPOFV

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IRB Barcelona / BIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

characteristic: genotype

text: GFP+; CD45-

characteristic: immunophenotype

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

text: Mus musculus

text: OK

text: HRKCTKTT


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IRB Barcelona / BIST

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: neoplastic cell

text: house mouse

characteristic: common name

text: adult

text: metastatic colorectal cancer

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

text: Mus musculus

text: OK

text: PFZRLMPV


text: IRB Barcelona / BIST

text: public

text: ArrayExpress

text: neoplastic cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: metastatic colorectal cancer

characteristic: disease

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

characteristic: organism

text: Mus musculus

text: liver micrometastasis

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Mus musculus

text: OK

characteristic: single cell well quality

text: OUKXWXTJ


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IRB Barcelona / BIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

text: Mus musculus

text: OK

characteristic: strain

text: QKPOAETA

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IRB Barcelona / BIST

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

characteristic: genotype

text: GFP+; CD45-

characteristic: immunophenotype

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

text: Mus musculus

text: OK

text: DEEJOPQB


text: IRB Barcelona / BIST

text: public

text: ArrayExpress

characteristic: broker name

text: neoplastic cell

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: metastatic colorectal cancer

characteristic: disease

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

characteristic: organism

text: Mus musculus

text: liver micrometastasis

characteristic: organism part

text: pass

text: Mus musculus

text: OK

text: LTYQDSWX


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IRB Barcelona / BIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

characteristic: post analysis well quality

text: Mus musculus

text: OK

characteristic: single cell well quality

text: IRVEVTBX


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IRB Barcelona / BIST

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

text: Mus musculus

text: OK

characteristic: strain

text: QPULBOUZ

characteristic: title


text: IRB Barcelona / BIST

text: public

text: ArrayExpress

characteristic: broker name

text: neoplastic cell

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: metastatic colorectal cancer

characteristic: disease

text: wild type genotype

characteristic: genotype

text: GFP+; CD45-

characteristic: immunophenotype

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

text: Mus musculus

text: OK

text: YMAZYFIL


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IRB Barcelona / BIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

characteristic: organism

text: Mus musculus

text: liver micrometastasis

characteristic: organism part

text: pass

text: Mus musculus

text: OK

text: DEKXGMRN


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IRB Barcelona / BIST

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

characteristic: post analysis well quality

text: Mus musculus

text: OK

characteristic: single cell well quality

text: KVELEXAQ


text: IRB Barcelona / BIST

text: public

text: ArrayExpress

characteristic: broker name

text: neoplastic cell

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: metastatic colorectal cancer

characteristic: disease

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

text: Mus musculus

text: OK

characteristic: strain

text: XTKGUHEV

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IRB Barcelona / BIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

characteristic: genotype

text: GFP+; CD45-

characteristic: immunophenotype

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

text: Mus musculus

text: OK

text: KRSXRIAX


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IRB Barcelona / BIST

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

characteristic: organism

text: Mus musculus

text: liver micrometastasis

characteristic: organism part

text: pass

text: Mus musculus

text: OK

text: CSPEEWLT


text: IRB Barcelona / BIST

text: public

text: ArrayExpress

characteristic: broker name

text: neoplastic cell

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: metastatic colorectal cancer

characteristic: disease

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

characteristic: post analysis well quality

text: Mus musculus

text: OK

characteristic: single cell well quality

text: PUAAYRKT


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IRB Barcelona / BIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

text: Mus musculus

text: OK

characteristic: strain

text: HDXFQZTB

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IRB Barcelona / BIST

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

characteristic: genotype

text: GFP+; CD45-

characteristic: immunophenotype

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

text: Mus musculus

text: OK

text: HZUXDLSV


text: IRB Barcelona / BIST

text: public

text: ArrayExpress

characteristic: broker name

text: neoplastic cell

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: metastatic colorectal cancer

characteristic: disease

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

characteristic: organism

text: Mus musculus

text: liver micrometastasis

characteristic: organism part

text: pass

text: Mus musculus

text: OK

text: NCFIPLPR


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IRB Barcelona / BIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

characteristic: post analysis well quality

text: Mus musculus

text: OK

characteristic: single cell well quality

text: XIQDWIUD


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IRB Barcelona / BIST

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

text: Mus musculus

text: OK

characteristic: strain

text: ZNEWDHQH

characteristic: title


text: IRB Barcelona / BIST

text: public

text: ArrayExpress

characteristic: broker name

text: neoplastic cell

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: metastatic colorectal cancer

characteristic: disease

text: wild type genotype

characteristic: genotype

text: GFP+; CD45-

characteristic: immunophenotype

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

text: Mus musculus

text: OK

text: RPJPZGGO


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IRB Barcelona / BIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

characteristic: organism

text: Mus musculus

text: liver micrometastasis

characteristic: organism part

text: pass

text: Mus musculus

text: OK

text: IALYAUHF


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IRB Barcelona / BIST

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

characteristic: post analysis well quality

text: Mus musculus

text: OK

characteristic: single cell well quality

text: WNUWRQUH


text: IRB Barcelona / BIST

text: public

text: ArrayExpress

characteristic: broker name

text: neoplastic cell

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: metastatic colorectal cancer

characteristic: disease

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

text: Mus musculus

text: OK

characteristic: strain

text: KZMAGNNO

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IRB Barcelona / BIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

characteristic: genotype

text: GFP+; CD45-

characteristic: immunophenotype

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

text: Mus musculus

text: OK

text: LFKWMCFY


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IRB Barcelona / BIST

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

characteristic: organism

text: Mus musculus

text: liver micrometastasis

characteristic: organism part

text: pass

text: Mus musculus

text: OK

text: YLYKEFLJ


text: IRB Barcelona / BIST

text: public

text: ArrayExpress

characteristic: broker name

text: neoplastic cell

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: metastatic colorectal cancer

characteristic: disease

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

characteristic: post analysis well quality

text: Mus musculus

text: OK

characteristic: single cell well quality

text: IXKRBKNY


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IRB Barcelona / BIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

text: Mus musculus

text: OK

characteristic: strain

text: RAZOCEAI

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IRB Barcelona / BIST

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

characteristic: genotype

text: GFP+; CD45-

characteristic: immunophenotype

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

text: Mus musculus

text: OK

text: FWVLCZYF


text: IRB Barcelona / BIST

text: public

text: ArrayExpress

characteristic: broker name

text: neoplastic cell

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: metastatic colorectal cancer

characteristic: disease

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

characteristic: organism

text: Mus musculus

text: liver micrometastasis

characteristic: organism part

text: pass

text: Mus musculus

text: OK

text: MDIFEORM


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IRB Barcelona / BIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

characteristic: post analysis well quality

text: Mus musculus

text: OK

characteristic: single cell well quality

text: YVRGLBYQ


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IRB Barcelona / BIST

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

text: Mus musculus

text: OK

characteristic: strain

text: OMEUARJG

characteristic: title


text: IRB Barcelona / BIST

text: public

text: ArrayExpress

characteristic: broker name

text: neoplastic cell

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: metastatic colorectal cancer

characteristic: disease

text: wild type genotype

characteristic: genotype

text: GFP+; CD45-

characteristic: immunophenotype

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

text: Mus musculus

text: OK

text: LLHLUYMD


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IRB Barcelona / BIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

characteristic: organism

text: Mus musculus

text: liver micrometastasis

characteristic: organism part

text: pass

text: Mus musculus

text: OK

text: TFTGUWCA


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IRB Barcelona / BIST

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

characteristic: post analysis well quality

text: Mus musculus

text: OK

characteristic: single cell well quality

text: CIBPBAKF


text: IRB Barcelona / BIST

text: public

text: ArrayExpress

characteristic: broker name

text: neoplastic cell

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: metastatic colorectal cancer

characteristic: disease

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

text: Mus musculus

text: OK

characteristic: strain

text: VQXPTSRS

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IRB Barcelona / BIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

characteristic: genotype

text: GFP+; CD45-

characteristic: immunophenotype

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

text: Mus musculus

text: OK

text: COQEMKIF


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IRB Barcelona / BIST

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

characteristic: organism

text: Mus musculus

text: liver micrometastasis

characteristic: organism part

text: pass

text: Mus musculus

text: OK

text: DIUXODJS


text: IRB Barcelona / BIST

text: public

text: ArrayExpress

characteristic: broker name

text: neoplastic cell

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: metastatic colorectal cancer

characteristic: disease

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

characteristic: post analysis well quality

text: Mus musculus

text: OK

characteristic: single cell well quality

text: BMUEVGYB


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IRB Barcelona / BIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

text: Mus musculus

text: OK

characteristic: strain

text: SRODLFAN

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IRB Barcelona / BIST

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

characteristic: genotype

text: GFP+; CD45-

characteristic: immunophenotype

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

text: Mus musculus

text: OK

text: YWOHEOSU


text: IRB Barcelona / BIST

text: public

text: ArrayExpress

characteristic: broker name

text: neoplastic cell

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: metastatic colorectal cancer

characteristic: disease

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

characteristic: organism

text: Mus musculus

text: liver micrometastasis

characteristic: organism part

text: pass

text: Mus musculus

text: OK

text: JXAWVNWC


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IRB Barcelona / BIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

characteristic: post analysis well quality

text: Mus musculus

text: OK

characteristic: single cell well quality

text: QLUTWUMZ


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IRB Barcelona / BIST

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

text: Mus musculus

text: OK

characteristic: strain

text: BXGGCOIE

characteristic: title


text: IRB Barcelona / BIST

text: public

text: ArrayExpress

characteristic: broker name

text: neoplastic cell

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: metastatic colorectal cancer

characteristic: disease

text: wild type genotype

characteristic: genotype

text: GFP+; CD45-

characteristic: immunophenotype

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

text: Mus musculus

text: OK

text: ITHQXMHX


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IRB Barcelona / BIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

characteristic: organism

text: Mus musculus

text: liver micrometastasis

characteristic: organism part

text: pass

text: Mus musculus

text: OK

text: OGGLLIGM


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IRB Barcelona / BIST

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

characteristic: post analysis well quality

text: Mus musculus

text: OK

characteristic: single cell well quality

text: UZPYCHKP


text: IRB Barcelona / BIST

text: public

text: ArrayExpress

characteristic: broker name

text: neoplastic cell

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: metastatic colorectal cancer

characteristic: disease

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

text: Mus musculus

text: OK

characteristic: strain

text: WFVTBBDV

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IRB Barcelona / BIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

characteristic: genotype

text: GFP+; CD45-

characteristic: immunophenotype

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

text: Mus musculus

text: OK

text: VZLQNWFU


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IRB Barcelona / BIST

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

characteristic: organism

text: Mus musculus

text: liver micrometastasis

characteristic: organism part

text: pass

text: Mus musculus

text: OK

text: WONVRLUV


text: IRB Barcelona / BIST

text: public

text: ArrayExpress

characteristic: broker name

text: neoplastic cell

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: metastatic colorectal cancer

characteristic: disease

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

characteristic: post analysis well quality

text: Mus musculus

text: OK

characteristic: single cell well quality

text: XPGCPZTR


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IRB Barcelona / BIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

text: Mus musculus

text: OK

characteristic: strain

text: UVXAHWEC

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IRB Barcelona / BIST

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

characteristic: genotype

text: GFP+; CD45-

characteristic: immunophenotype

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

text: Mus musculus

text: OK

text: DKNRPOEC


text: IRB Barcelona / BIST

text: public

text: ArrayExpress

characteristic: broker name

text: neoplastic cell

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: metastatic colorectal cancer

characteristic: disease

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

characteristic: organism

text: Mus musculus

text: liver micrometastasis

characteristic: organism part

text: pass

text: Mus musculus

text: OK

text: EUKJADZN


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IRB Barcelona / BIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

characteristic: post analysis well quality

text: Mus musculus

text: OK

characteristic: single cell well quality

text: ILBBLGDJ


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IRB Barcelona / BIST

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

text: Mus musculus

text: OK

characteristic: strain

text: NZYTHYUS

characteristic: title


text: IRB Barcelona / BIST

text: public

text: ArrayExpress

characteristic: broker name

text: neoplastic cell

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: metastatic colorectal cancer

characteristic: disease

text: wild type genotype

characteristic: genotype

text: GFP+; CD45-

characteristic: immunophenotype

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

text: Mus musculus

text: OK

text: TRERGXXV


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IRB Barcelona / BIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

characteristic: organism

text: Mus musculus

text: liver micrometastasis

characteristic: organism part

text: pass

text: Mus musculus

text: OK

text: HPUERFQA


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IRB Barcelona / BIST

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

characteristic: post analysis well quality

text: Mus musculus

text: OK

characteristic: single cell well quality

text: MQVUIVGP


text: IRB Barcelona / BIST

text: public

text: ArrayExpress

characteristic: broker name

text: neoplastic cell

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: metastatic colorectal cancer

characteristic: disease

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

text: Mus musculus

text: OK

characteristic: strain

text: LRCENTGJ

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IRB Barcelona / BIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

characteristic: genotype

text: GFP+; CD45-

characteristic: immunophenotype

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

text: Mus musculus

text: OK

text: QMBBOGGK


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IRB Barcelona / BIST

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

characteristic: organism

text: Mus musculus

text: liver micrometastasis

characteristic: organism part

text: pass

text: Mus musculus

text: OK

text: HULZSUFK


text: IRB Barcelona / BIST

text: public

text: ArrayExpress

characteristic: broker name

text: neoplastic cell

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: metastatic colorectal cancer

characteristic: disease

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

characteristic: post analysis well quality

text: Mus musculus

text: OK

characteristic: single cell well quality

text: VDOUOEFT


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IRB Barcelona / BIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

text: Mus musculus

text: OK

characteristic: strain

text: SNPBZLPY

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IRB Barcelona / BIST

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

characteristic: genotype

text: GFP+; CD45-

characteristic: immunophenotype

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

text: Mus musculus

text: OK

text: ECGCCTBJ


text: IRB Barcelona / BIST

text: public

text: ArrayExpress

characteristic: broker name

text: neoplastic cell

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: metastatic colorectal cancer

characteristic: disease

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

characteristic: organism

text: Mus musculus

text: liver micrometastasis

characteristic: organism part

text: pass

text: Mus musculus

text: OK

text: JILGHXVO


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IRB Barcelona / BIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

characteristic: post analysis well quality

text: Mus musculus

text: OK

characteristic: single cell well quality

text: MJNUWYMJ


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IRB Barcelona / BIST

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ArrayExpress

text: neoplastic cell

text: house mouse

text: adult

text: metastatic colorectal cancer

text: wild type genotype

text: GFP+; CD45-

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

text: Mus musculus

text: OK

characteristic: strain

text: EJJVFIBU

characteristic: title


text: IRB Barcelona / BIST

text: public

text: ArrayExpress

characteristic: broker name

text: neoplastic cell

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: metastatic colorectal cancer

characteristic: disease

text: wild type genotype

characteristic: genotype

text: GFP+; CD45-

characteristic: immunophenotype

text: Mus musculus

text: Mus musculus

text: liver micrometastasis

text: pass

text: Mus musculus

text: OK

text: MJXFJRCY


text: Plasmodium falciparum

characteristic: ArrayExpress-SPECIES

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: malaria parasite P. falciparum

text: Plasmodium falciparum

characteristic: organism

text: Plasmodium falciparum

text: Plasmodium falciparum


name: E-MTAB-12126:Rm1021 ECF/anti-σ-free strain overexpressing ecf02_2817 rep1

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

tag: Namespace:Philipps-Universitat Marburg Center for Synthetic Microbiology (Synmikro) Becker lab

characteristic: Submitter Id

text: ArrayExpress

text: Protocols: Rm1021 ECF/anti-σ-free strain derivatives were grown to an OD600 of 0.1-0.3 in MOPS buffered minimal medium with gentamicin (6 µg/mL). The OD600 was adjusted to 0.1 and IPTG was added at a final concentration of 500 µM. Strains were grown for ~ 8-9 hours until cell density reached an OD600 of 0.8 and collected by centrifugation at 10,000 rcf for 5 min. Cell pellets were frozen in liquid nitrogen and kept at -80 °C. Assays were performed in triplicates for each strain. For total RNA isolation, the Qiagen miRNeasy Kit was used starting from a pellet of 8 mL cultured cells. The cell pellet was resuspended in 1 mL QIAzol lysis reagent and homogenized in a FastPrep® sample preparation system using Lysing Matrix B containing 0,1 mm silica beads (MP biomedicals) and the following settings: 3x 6500 rpm for 20 sec, 15 sec break. The supernatant was transferred into a new tube and RNA isolation was performed according to the manufacturer’s instruction including the optional on column DNaseI digestion. rRNA depletion was conducted with the Illumina Ribo-Zero rRNA removal Kit (Bacteria). The cDNA library was prepared using the NEB Ultra directional Kit.

text: vegetative growth

text: Rm1021 Δecf/anti-σ pSRKGm [m] Plac/T5 ecf02_2817

text: Sinorhizobium meliloti

text: Sinorhizobium meliloti

text: whole organism

characteristic: organism part

text: Sinorhizobium meliloti


name: E-MTAB-12126:Rm1021 ECF/anti-σ-free strain overexpressing ecf02_2817 rep2

text: Philipps-Universitat Marburg Center for Synthetic Microbiology (Synmikro) Becker lab

text: public

text: ArrayExpress

characteristic: broker name

characteristic: description

text: vegetative growth

characteristic: developmental stage

text: Sinorhizobium meliloti

text: Sinorhizobium meliloti

text: whole organism

text: Sinorhizobium meliloti

characteristic: strain

characteristic: title


name: E-MTAB-12126:Rm1021 ECF/anti-σ-free strain overexpressing ecf02_2817 rep3

characteristic: ENA-CHECKLIST

text: public

text: ArrayExpress

text: vegetative growth

characteristic: genotype

text: Sinorhizobium meliloti

text: Sinorhizobium meliloti

text: whole organism

text: Sinorhizobium meliloti


name: E-MTAB-12126:Rm1021 ECF/anti-σ-free strain overexpressing ecf11_0987 rep1

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC last update

text: public

text: ArrayExpress

text: vegetative growth

text: Rm1021 Δecf/anti-σ pSRKGm [m] Plac/T5 ecf11_0987

text: Sinorhizobium meliloti

text: Sinorhizobium meliloti

text: whole organism

text: Sinorhizobium meliloti


name: E-MTAB-12126:Rm1021 ECF/anti-σ-free strain overexpressing ecf11_0987 rep2

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: vegetative growth

text: Sinorhizobium meliloti

text: Sinorhizobium meliloti

text: whole organism

text: Sinorhizobium meliloti


name: E-MTAB-12126:Rm1021 ECF/anti-σ-free strain overexpressing ecf11_0987 rep3

characteristic: ENA-LAST-UPDATE

text: public

text: ArrayExpress

text: vegetative growth

text: Sinorhizobium meliloti

characteristic: organism

text: Sinorhizobium meliloti

text: whole organism

text: Sinorhizobium meliloti


name: E-MTAB-12126:Rm1021 ECF/anti-σ-free strain with empty vector rep1

characteristic: External Id

characteristic: INSDC center name

text: public

text: ArrayExpress

text: vegetative growth

text: Rm1021 Δecf/anti-σ pSRKGm [m]

text: Sinorhizobium meliloti

text: Sinorhizobium meliloti

text: whole organism

text: Sinorhizobium meliloti


name: E-MTAB-12126:Rm1021 ECF/anti-σ-free strain with empty vector rep2

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ArrayExpress

text: vegetative growth

text: Rm1021 Δecf/anti-σ pSRKGm [m]

text: Sinorhizobium meliloti

text: Sinorhizobium meliloti

text: whole organism

text: Sinorhizobium meliloti


name: E-MTAB-12126:Rm1021 ECF/anti-σ-free strain with empty vector rep3

text: public

text: ArrayExpress

characteristic: broker name

text: vegetative growth

text: Rm1021 Δecf/anti-σ pSRKGm [m]

text: Sinorhizobium meliloti

text: Sinorhizobium meliloti

text: whole organism

characteristic: organism part

text: Sinorhizobium meliloti


name: E-MTAB-12127:Rm1021 ECF/anti-σ-free strain overexpressing ecf02_2817

text: public

text: ArrayExpress

text: Protocols: Rm1021 ECF/anti-σ-free strain derivatives were grown to an OD600 of 0.1-0.3 in MOPS buffered minimal medium with gentamicin (6 µg/mL). The OD600 was adjusted to 0.1 and IPTG was added at a final concentration of 500 µM. Strains were grown for ~ 8-9 hours until cell density reached an OD600 of 0.8 and collected by centrifugation at 10,000 rcf for 5 min. Cell pellets were frozen in liquid nitrogen and kept at -80 °C. Assays were performed in triplicates for each strain. For total RNA isolation, the Qiagen miRNeasy Kit was used starting from a pellet of 8 mL cultured cells. The cell pellet was resuspended in 1 mL QIAzol lysis reagent and homogenized in a FastPrep® sample preparation system using Lysing Matrix B containing 0.1 mm silica beads (MP biomedicals) and the following settings: 3x 6500 rpm for 20 sec, 15 sec break. The supernatant was transferred into a new tube and RNA isolation was performed according to the manufacturer’s instruction including the optional on column DNaseI digestion. RNAs from biological triplicates were pooled in equal amounts before library preparation. 5’-triphosphorylated RNAs were capped with a 3’-desthiobiotin-TEG-guanosine 5’-triphosphate (DTBGTP) using the vaccinia capping enzyme (VCE) (NEB). Biotinylated RNAs were captured on streptavidin beads and eluted to obtain the 5’-fragments of primary transcripts. The eluted RNAs were poly(A)-tailed using poly(A) polymerase. To distinguish primary transcripts from contaminating processed or degraded RNA molecules with a 5’-monophosphate, they were differentially labelled with identifying sequence tags (TSS-tag and PSS-tag, respectively): the 5' Illumina TruSeq sequencing adapters, which carry sequence tags ATTACTCG and TCCGGAGA, were ligated to the 5'-monophosphate groups of processed transcripts. The samples were then treated with CapClip Acid Pyrophosphatase (Cellscript) in order to convert 5' triphosphate structures into 5'-monophosphate ends. To the newly formed 5’-monophosphate groups 5' Illumina TruSeq sequencing adapters, carrying sequence tag CGCTCATT and GAGATTCC were ligated. The resulting cDNAs were PCR-amplified to yield 10-20 ng/µL (16 cycles) using a high fidelity polymerase. The full length cDNAs were degraded using dsDNA fragmentase (NEB), 100-300 bp long fragments were isolated and then bound to streptavidin magnetic beads. The bound cDNAs were blunted and 3’ Illumina sequencing adapter were ligated to the 3’ ends of the cDNA fragments. The bead bound cDNAs were PCR-amplified and barcoded. Barcoded cDNA libraries were pooled in equimolar amounts. The cDNA pool was size fractionated in the size range of 200-500 bp with the Agencourt AMPure kit.

characteristic: description

text: vegetative growth

characteristic: developmental stage

text: Sinorhizobium meliloti

text: Sinorhizobium meliloti

text: whole organism

text: Sinorhizobium meliloti


name: E-MTAB-12127:Rm1021 ECF/anti-σ-free strain overexpressing ecf11_0987

text: public

text: ArrayExpress

text: vegetative growth

text: Sinorhizobium meliloti

text: Sinorhizobium meliloti

text: whole organism

text: Sinorhizobium meliloti

characteristic: strain

characteristic: title


name: E-MTAB-12127:Rm1021 ECF/anti-σ-free strain with empty vector

characteristic: ENA-CHECKLIST

text: public

text: ArrayExpress

text: vegetative growth

text: Rm1021 Δecf/anti-σ pSRKGm [m]

characteristic: genotype

text: Sinorhizobium meliloti

text: Sinorhizobium meliloti

text: whole organism

text: Sinorhizobium meliloti


characteristic: ENA-FIRST-PUBLIC

text: Nees Institute

characteristic: INSDC last update

text: public

tag: Namespace:Nees Institute

characteristic: collection date

text: cultivated at Bonn Botanic Gardens

text: Greece

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: CTAB isolation

characteristic: isolation and growth condition

text: Leopoldia neumannii

text: adult

characteristic: plant developmental stage

text: soil

characteristic: plant growth medium

text: leaf tissue

characteristic: plant structure

text: Leopoldia neumannii

text: Genomic phylogeny of Muscari


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Nees Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: cultivated at Bonn Botanic Gardens

text: Georgia

text: CTAB isolation

text: Muscari armeniacum

characteristic: organism

text: adult

text: soil

text: leaf tissue

text: Muscari armeniacum

text: Genomic phylogeny of Muscari


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Nees Institute

characteristic: INSDC last update

text: public

text: cultivated at Bonn Botanic Gardens

characteristic: description

text: Turkey

text: CTAB isolation

text: Muscari parviflorum

text: adult

text: soil

text: leaf tissue

text: Muscari parviflorum

text: Genomic phylogeny of Muscari

characteristic: title


text: Nees Institute

text: public

characteristic: collection date

text: cultivated at Bonn Botanic Gardens

text: Greece

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: CTAB isolation

characteristic: isolation and growth condition

text: Leopoldia spreitzenhoferi

text: adult

text: soil

text: leaf tissue

text: Leopoldia spreitzenhoferi

text: Genomic phylogeny of Muscari


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Nees Institute

text: public

text: cultivated at Bonn Botanic Gardens

text: Turkey

text: CTAB isolation

text: Muscari mirum

text: adult

characteristic: plant developmental stage

text: soil

characteristic: plant growth medium

text: leaf tissue

characteristic: plant structure

text: Muscari mirum

text: Genomic phylogeny of Muscari


text: Nees Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: cultivated at Bonn Botanic Gardens

text: Turkey

text: CTAB isolation

text: Muscari massayanum

text: adult

text: soil

text: leaf tissue

text: Muscari massayanum

text: Genomic phylogeny of Muscari


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Nees Institute

characteristic: INSDC last update

text: public

text: cultivated at Bonn Botanic Gardens

characteristic: description

text: Turkey

text: CTAB isolation

text: Muscari macrocarpum

characteristic: organism

text: adult

text: soil

text: leaf tissue

text: Muscari macrocarpum

text: Genomic phylogeny of Muscari


text: Nees Institute

text: public

characteristic: collection date

text: cultivated at Bonn Botanic Gardens

text: Greece

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: CTAB isolation

text: Muscari neglectum

text: adult

text: soil

text: leaf tissue

text: Muscari neglectum

text: Genomic phylogeny of Muscari

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Nees Institute

text: public

text: cultivated at Bonn Botanic Gardens

text: Iran

text: CTAB isolation

characteristic: isolation and growth condition

text: Pseudomuscari chalusicum

text: adult

characteristic: plant developmental stage

text: soil

characteristic: plant growth medium

text: leaf tissue

text: Pseudomuscari chalusicum

text: Genomic phylogeny of Muscari


text: Nees Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: cultivated at Bonn Botanic Gardens

text: Greece

text: CTAB isolation

text: Leopoldia comosa

text: adult

text: soil

text: leaf tissue

characteristic: plant structure

text: Leopoldia comosa

text: Genomic phylogeny of Muscari


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Nees Institute

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: cultivated at Bonn Botanic Gardens

characteristic: description

text: Turkey

text: CTAB isolation

text: Muscari racemosum

text: adult

text: soil

text: leaf tissue

text: Muscari racemosum

text: Genomic phylogeny of Muscari


text: Nees Institute

text: public

characteristic: collection date

text: cultivated at Bonn Botanic Gardens

text: Turkey

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: CTAB isolation

text: Muscari bourgaei

characteristic: organism

text: adult

text: soil

text: leaf tissue

text: Muscari bourgaei

text: Genomic phylogeny of Muscari


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Nees Institute

text: public

text: cultivated at Bonn Botanic Gardens

text: Russia

text: CTAB isolation

characteristic: isolation and growth condition

text: Pseudomuscari coeruleum

text: adult

characteristic: plant developmental stage

text: soil

text: leaf tissue

text: Pseudomuscari coeruleum

text: Genomic phylogeny of Muscari

characteristic: title


text: Nees Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: cultivated at Bonn Botanic Gardens

text: Georgia

text: CTAB isolation

text: Leopoldia caucasica

text: adult

text: soil

characteristic: plant growth medium

text: leaf tissue

text: Leopoldia caucasica

text: Genomic phylogeny of Muscari


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Nees Institute

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: cultivated at Bonn Botanic Gardens

text: Georgia

text: CTAB isolation

text: Leopoldia tenuiflora

text: adult

text: soil

text: leaf tissue

characteristic: plant structure

text: Leopoldia tenuiflora

text: Genomic phylogeny of Muscari


text: Nees Institute

text: public

characteristic: collection date

text: cultivated at Bonn Botanic Gardens

characteristic: description

text: Turkey

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: CTAB isolation

text: Muscari latifolium

text: adult

text: soil

text: leaf tissue

text: Muscari latifolium

text: Genomic phylogeny of Muscari


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Nees Institute

text: public

text: cultivated at Bonn Botanic Gardens

text: Turkey

text: CTAB isolation

characteristic: isolation and growth condition

text: Muscari discolor

characteristic: organism

text: adult

characteristic: plant developmental stage

text: soil

text: leaf tissue

text: Muscari discolor

text: Genomic phylogeny of Muscari


text: Nees Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: cultivated at Bonn Botanic Gardens

text: Georgia

text: CTAB isolation

text: Pseudomuscari pallens

text: adult

text: soil

text: leaf tissue

text: Pseudomuscari pallens

text: Genomic phylogeny of Muscari

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Nees Institute

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: cultivated at Bonn Botanic Gardens

text: Turkey

text: CTAB isolation

text: Pseudomuscari azureum

text: adult

text: soil

characteristic: plant growth medium

text: leaf tissue

text: Pseudomuscari azureum

text: Genomic phylogeny of Muscari


text: Nees Institute

text: public

characteristic: collection date

text: cultivated at Bonn Botanic Gardens

characteristic: description

text: Turkey

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: CTAB isolation

text: Bellevalia paradoxa

text: adult

text: soil

text: leaf tissue

characteristic: plant structure

text: Bellevalia paradoxa

text: Genomic phylogeny of Muscari


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Nees Institute

text: public

text: cultivated at Bonn Botanic Gardens

text: Morocco

characteristic: geographic location (longitude)

text: CTAB isolation

characteristic: isolation and growth condition

text: Leopoldia comosa

characteristic: organism

text: adult

text: soil

text: leaf tissue

text: Leopoldia comosa

text: Genomic phylogeny of Muscari


text: Nees Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: cultivated at Bonn Botanic Gardens

text: Turkey

text: CTAB isolation

text: Muscari sivrihisardaghlarense

text: adult

characteristic: plant developmental stage

text: soil

text: leaf tissue

text: Muscari sivrihisardaghlarense

text: Genomic phylogeny of Muscari


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Nees Institute

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: Greece

text: CTAB isolation

text: Muscari botryoides

text: adult

text: soil

text: leaf tissue

text: Muscari botryoides

text: Genomic phylogeny of Muscari

characteristic: title


text: Nees Institute

text: public

characteristic: collection date

text: cultivated at Bonn Botanic Gardens

characteristic: description

text: Greece

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: CTAB isolation

text: Muscari kerkis

text: adult

text: soil

characteristic: plant growth medium

text: leaf tissue

text: Muscari kerkis

text: Genomic phylogeny of Muscari


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Nees Institute

text: public

text: Spain

characteristic: geographic location (longitude)

text: CTAB isolation

characteristic: isolation and growth condition

text: Muscari baeticum

text: adult

text: soil

text: leaf tissue

characteristic: plant structure

text: Muscari baeticum

text: Genomic phylogeny of Muscari


text: Nees Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Spain

text: CTAB isolation

text: Muscari matritense

characteristic: organism

text: adult

text: soil

text: leaf tissue

text: Muscari matritense

text: Genomic phylogeny of Muscari


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Nees Institute

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: cultivated at Bonn Botanic Gardens

text: Greece

text: CTAB isolation

text: Leopoldia neumannii

text: adult

characteristic: plant developmental stage

text: soil

text: leaf tissue

text: Leopoldia neumannii

text: Genomic phylogeny of Muscari


text: Nees Institute

text: public

characteristic: collection date

text: cultivated at Bonn Botanic Gardens

characteristic: description

text: Greece

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: CTAB isolation

text: Muscari pulchellum

text: adult

text: soil

text: leaf tissue

text: Muscari pulchellum

text: Genomic phylogeny of Muscari

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Nees Institute

text: public

text: cultivated at Bonn Botanic Gardens

text: Turkey

characteristic: geographic location (longitude)

text: CTAB isolation

characteristic: isolation and growth condition

text: Muscari vuralii

text: adult

text: soil

characteristic: plant growth medium

text: leaf tissue

text: Muscari vuralii

text: Genomic phylogeny of Muscari


text: Nees Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: cultivated at Bonn Botanic Gardens

text: Iran

text: CTAB isolation

text: Leopoldia longipes

text: adult

text: soil

text: leaf tissue

characteristic: plant structure

text: Leopoldia longipes

text: Genomic phylogeny of Muscari


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Nees Institute

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: cultivated at Bonn Botanic Gardens

text: Iraq

text: CTAB isolation

text: Muscari inconstrictum

characteristic: organism

text: adult

text: soil

text: leaf tissue

text: Muscari inconstrictum

text: Genomic phylogeny of Muscari


text: Nees Institute

text: public

characteristic: collection date

text: cultivated at Bonn Botanic Gardens

characteristic: description

text: Greece

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: CTAB isolation

text: Muscari commutatum

text: adult

characteristic: plant developmental stage

text: soil

text: leaf tissue

text: Muscari commutatum

text: Genomic phylogeny of Muscari


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Nees Institute

text: public

text: cultivated at Bonn Botanic Gardens

text: Portugal

characteristic: geographic location (longitude)

text: CTAB isolation

characteristic: isolation and growth condition

text: Leopoldia comosa

text: adult

text: soil

text: leaf tissue

text: Leopoldia comosa

text: Genomic phylogeny of Muscari

characteristic: title


text: Nees Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: cultivated at Bonn Botanic Gardens

text: Greece

text: CTAB isolation

text: Leopoldia weissii

text: adult

text: soil

characteristic: plant growth medium

text: leaf tissue

text: Leopoldia weissii

text: Genomic phylogeny of Muscari


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Nees Institute

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: cultivated at Bonn Botanic Gardens

text: Greece

text: CTAB isolation

text: Leopoldia cycladica

text: adult

text: soil

text: leaf tissue

characteristic: plant structure

text: Leopoldia cycladica

text: Genomic phylogeny of Muscari


text: Nees Institute

text: public

characteristic: collection date

text: cultivated at Bonn Botanic Gardens

characteristic: description

text: Greece

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: CTAB isolation

text: Bellevalia dubia

characteristic: organism

text: adult

text: soil

text: leaf tissue

text: Bellevalia dubia

text: Genomic phylogeny of Muscari


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Nees Institute

text: public

text: cultivated at Bonn Botanic Gardens

text: Spain

characteristic: geographic location (longitude)

text: CTAB isolation

characteristic: isolation and growth condition

text: Brimeura amethystina

text: adult

characteristic: plant developmental stage

text: soil

text: leaf tissue

text: Brimeura amethystina

text: Genomic phylogeny of Muscari


text: Nees Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: cultivated at Bonn Botanic Gardens

text: Georgia

text: CTAB isolation

text: Bellevalia speciosa

text: adult

text: soil

text: leaf tissue

text: Bellevalia speciosa

text: Genomic phylogeny of Muscari

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Nees Institute

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: cultivated at Bonn Botanic Gardens

text: Turkey

text: CTAB isolation

text: Muscari anatolicum

text: adult

text: soil

characteristic: plant growth medium

text: leaf tissue

text: Muscari anatolicum

text: Genomic phylogeny of Muscari


text: Nees Institute

text: public

characteristic: collection date

text: cultivated at Bonn Botanic Gardens

characteristic: description

text: Turkey

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: CTAB isolation

text: Muscari armeniacum

text: adult

text: soil

text: leaf tissue

characteristic: plant structure

text: Muscari armeniacum

text: Genomic phylogeny of Muscari


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Nees Institute

text: public

text: cultivated at Bonn Botanic Gardens

text: Turkey

characteristic: geographic location (longitude)

text: CTAB isolation

characteristic: isolation and growth condition

text: Muscari adilii

characteristic: organism

text: adult

text: soil

text: leaf tissue

text: Muscari adilii

text: Genomic phylogeny of Muscari


text: Ghent university

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Ghent university

text: Commensalibacter sp. R-53529 isolated from the bumble bee gut

text: Belgium

characteristic: isolate

text: Commensalibacter sp. LMG 31819

text: Commensalibacter sp. LMG 31819

characteristic: strain

text: Commensalibacter sp. R-53529


LMG 28296

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Ghent university

characteristic: INSDC last update

text: public

text: LMG 28296

characteristic: Submitter Id

text: Commensalibacter sp. LMG 28296 isolated from the bumble bee gut

text: Belgium

text: LMG 28296

text: Commensalibacter sp. LMG 31819

text: Commensalibacter sp. LMG 31819

text: LMG 28296

text: Commensalibacter sp. LMG 28296

characteristic: title


LMG 31819

text: Ghent university

text: public

text: LMG 31819

text: Commensalibacter sp. LMG 31819 isolated from the bumble bee gut

characteristic: description

text: Belgium

characteristic: geographic location (country and/or sea)

text: LMG 31819

text: Commensalibacter sp. LMG 31819

text: Commensalibacter sp. LMG 31819

text: LMG 31819

text: Commensalibacter sp. LMG 31819


LMG 32512

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Ghent university

text: public

text: LMG 32512

text: Commensalibacter sp. LMG 32512 isolated from the hornet gut

text: Belgium

text: LMG 32512

text: Commensalibacter sp. LMG 32512

text: Commensalibacter sp. LMG 32512

text: LMG 32512

text: Commensalibacter sp. LMG 32512


text: Ghent university

text: public

text: Commensalibacter sp. R-83540 isolated from the hornet gut

text: Belgium

text: Commensalibacter sp. LMG 31819

text: Commensalibacter sp. LMG 31819

text: Commensalibacter sp. R-83540


text: Ghent university

text: public

text: Commensalibacter sp. R-83526 isolated from the hornet gut

text: Belgium

text: Commensalibacter sp. LMG 31819

text: Commensalibacter sp. LMG 31819

text: Commensalibacter sp. R-83526


text: Ghent university

text: public

text: Commensalibacter sp. R-83534 isolated from the butterfly gut

text: Belgium

text: Commensalibacter sp. LMG 32512

text: Commensalibacter sp. LMG 32512

text: Commensalibacter sp. R-83534


text: Ghent university

text: public

text: Commensalibacter sp. R-83493 isolated from the butterfly gut

text: Belgium

text: Commensalibacter sp. LMG 31819

text: Commensalibacter sp. LMG 31819

text: Commensalibacter sp. R-83493


text: Plasmodium falciparum

characteristic: ArrayExpress-SPECIES

text: SC

text: public

text: malaria parasite P. falciparum

characteristic: common name

text: Plasmodium falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum


characteristic: INSDC center name

text: public

tag: Namespace:Senckenber Biodiversity and Climate Research Centre Frankfurt

text: Chironomus riparius

characteristic: organism

text: Chironomus riparius


text: Senckenber Biodiversity and Climate Research Centre Frankfurt

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: Chironomus riparius

text: Chironomus riparius


text: public

characteristic: SRA accession

tag: Namespace:FRIEDRICH-LOEFFLER-INSTITUT (FLI) Institute for Bacterial Infections and Zoonoses (IBIZ)

text: Campylobacter jejuni from commercial turkey in Germany

text: Germany

text: Campylobacter jejuni

text: Campylobacter jejuni

text: Campylobacter jejuni


text: FRIEDRICH-LOEFFLER-INSTITUT (FLI) Institute for Bacterial Infections and Zoonoses (IBIZ)

text: public

text: Germany

text: Campylobacter jejuni

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

tag: Namespace:University of South Bohemia, Faculty of Science

text: agricultural field

characteristic: broad-scale environmental context

characteristic: collection date

unit: m

characteristic: depth

text: Bacterial V4 amplicons from agricultural field soil after a long-term lab incubation experiment

characteristic: elevation

text: chernozem

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: soil

characteristic: local environmental context

text: soil metagenome

text: eroSOM

characteristic: project name

text: soil metagenome

text: Illumina NovaSeq 6000

characteristic: sequencing method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of South Bohemia, Faculty of Science

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: agricultural field

characteristic: description

text: chernozem

text: Czech Republic

characteristic: geographic location (country and/or sea)

text: soil

text: soil metagenome

text: eroSOM

text: soil metagenome

text: Illumina NovaSeq 6000

characteristic: title


characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: agricultural field

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

text: chernozem

text: Germany

text: soil

text: soil metagenome

characteristic: organism

text: eroSOM

text: soil metagenome

text: Illumina NovaSeq 6000


characteristic: ENA-CHECKLIST

text: public

text: agricultural field

text: Fungal ITS amplicons from agricultural field soil after a long-term lab incubation experiment

characteristic: elevation

text: chernozem

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: soil

characteristic: local environmental context

text: soil metagenome

text: eroSOM

characteristic: project name

text: soil metagenome

text: Illumina NovaSeq 6000

characteristic: sequencing method


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: agricultural field

characteristic: description

text: chernozem

text: Germany

characteristic: geographic location (country and/or sea)

text: soil

text: soil metagenome

text: eroSOM

text: soil metagenome

text: Illumina NovaSeq 6000

characteristic: title


E-MTAB-12130:Female 1

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12130:Female 1

tag: Namespace:Departamento de Produccion Animal, Facultad de Veterinaria, Universidad de Leon

unit: day

characteristic: age

text: Assaf

characteristic: breed

text: ArrayExpress

characteristic: broker name

text: sheep

characteristic: common name

text: Protocols: Perirenal fat samples were collected from 12 Assaf suckling lambs (6 males and 6 females). All animals were born at the Instituto de Ganadería de Montaña (IGM) (Grulleros, León, Castilla y León, Spain) in the same lambing season (winter 2019-2020) from primiparous Assaf dairy ewes. The lambs were reared under the same management and diet conditions. Initially, during the first 24 hours after lambing, the lambs were kept with their dams to suck the colostrum. Subsequently, the animals were held in lamb pens fed with artificial milk using Ovilac 60 (Calfvet®) milk replacer powder ad libitum up to slaughter to remove the influence of maternal effects. The lambs were slaughtered at a local slaughterhouse at the market weight for the PGI label “lechazo de Castilla y Leon” (9-12 kg), which has average ages of 28.16 (±4.87) and 24.16 (±5.19) for males and females, respectively. The management, transport and sacrifice of the animals were in accordance with Spanish and EU legislation [Spanish Laws 32/2007, 6/2013 and RD 37/2014; Council Regulation (EC) 199/2009]. For RNA extraction, the sampled tissues were preserved in an RNA-stabilization solution (Ambion RNAlater; Life Technologies) and stored at 4 °C for 24 h. Subsequently, the RNA-stabilization solution was removed, and the samples were frozen at -80 °C until RNA extraction. RNA was extracted using the miRNeasy Mini KIT (Qiagen, Germany) with adaptations for use in adipose tissue. For RNA extraction, the sampled tissues were preserved in an RNA-stabilization solution (Ambion RNAlater; Life Technologies) and stored at 4 °C for 24 h. Subsequently, the RNA-stabilization solution was removed, and the samples were frozen at -80 °C until RNA extraction. RNA was extracted using the miRNeasy Mini KIT (Qiagen, Germany) with adaptations for use in adipose tissue.

text: infant

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

characteristic: individual

text: Ovis aries

characteristic: organism

text: Ovis aries

text: perirenal fat

characteristic: organism part

text: Ovis aries

text: female

characteristic: sex

text: Female 1


E-MTAB-12130:Female 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Departamento de Produccion Animal, Facultad de Veterinaria, Universidad de Leon

characteristic: INSDC last update

text: public

text: E-MTAB-12130:Female 2

characteristic: Submitter Id

text: Assaf

text: ArrayExpress

text: sheep

characteristic: description

text: infant

text: normal

text: wild type genotype

text: Ovis aries

text: Ovis aries

text: perirenal fat

text: Ovis aries

text: female

text: Female 2


E-MTAB-12130:Female 3

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12130:Female 3

characteristic: age

text: Assaf

characteristic: breed

text: ArrayExpress

text: sheep

text: infant

text: normal

text: wild type genotype

text: Ovis aries

text: Ovis aries

text: perirenal fat

text: Ovis aries

text: female

text: Female 3

characteristic: title


E-MTAB-12130:Female 4

text: public

text: E-MTAB-12130:Female 4

text: Assaf

text: ArrayExpress

characteristic: broker name

text: sheep

characteristic: common name

text: infant

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

characteristic: individual

text: Ovis aries

characteristic: organism

text: Ovis aries

text: perirenal fat

text: Ovis aries

text: female

text: Female 4


E-MTAB-12130:Female 5

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC last update

text: public

text: E-MTAB-12130:Female 5

text: Assaf

text: ArrayExpress

text: sheep

text: infant

text: normal

text: wild type genotype

text: Ovis aries

text: Ovis aries

text: perirenal fat

characteristic: organism part

text: Ovis aries

text: female

characteristic: sex

text: Female 5


E-MTAB-12130:Female 6

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12130:Female 6

characteristic: Submitter Id

characteristic: age

text: Assaf

text: ArrayExpress

text: sheep

characteristic: description

text: infant

text: normal

text: wild type genotype

text: Ovis aries

text: Ovis aries

text: perirenal fat

text: Ovis aries

text: female

text: Female 6


E-MTAB-12130:Male 1

text: public

text: E-MTAB-12130:Male 1

text: Assaf

characteristic: breed

text: ArrayExpress

characteristic: broker name

text: sheep

characteristic: common name

text: infant

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: Ovis aries

text: Ovis aries

text: perirenal fat

text: Ovis aries

text: male

text: Male 1

characteristic: title


E-MTAB-12130:Male 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC last update

text: public

text: E-MTAB-12130:Male 2

text: Assaf

text: ArrayExpress

text: sheep

text: infant

text: normal

text: wild type genotype

characteristic: individual

text: Ovis aries

characteristic: organism

text: Ovis aries

text: perirenal fat

text: Ovis aries

text: male

text: Male 2


E-MTAB-12130:Male 3

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12130:Male 3

characteristic: Submitter Id

text: Assaf

text: ArrayExpress

text: sheep

text: infant

text: normal

text: wild type genotype

text: Ovis aries

text: Ovis aries

text: perirenal fat

characteristic: organism part

text: Ovis aries

text: male

characteristic: sex

text: Male 3


E-MTAB-12130:Male 4

text: public

text: E-MTAB-12130:Male 4

characteristic: age

text: Assaf

characteristic: breed

text: ArrayExpress

characteristic: broker name

text: sheep

characteristic: common name

characteristic: description

text: infant

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

text: Ovis aries

text: Ovis aries

text: perirenal fat

text: Ovis aries

text: male

text: Male 4


E-MTAB-12130:Male 5

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC last update

text: public

text: E-MTAB-12130:Male 5

text: Assaf

text: ArrayExpress

text: sheep

text: infant

text: normal

text: wild type genotype

characteristic: genotype

text: Ovis aries

text: Ovis aries

text: perirenal fat

text: Ovis aries

text: male

text: Male 5

characteristic: title


E-MTAB-12130:Male 6

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12130:Male 6

characteristic: Submitter Id

text: Assaf

text: ArrayExpress

text: sheep

text: infant

text: normal

text: wild type genotype

characteristic: individual

text: Ovis aries

characteristic: organism

text: Ovis aries

text: perirenal fat

text: Ovis aries

text: male

text: Male 6


CTRL

text: UMCG

text: public

text: CTRL

text: human

characteristic: common name

text: CTRL

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: CTRL


CTRL-CIS

text: UMCG

text: public

text: CTRL-CIS

text: human

text: CTRL-CIS

text: Homo sapiens

text: Homo sapiens

text: CTRL-CIS


text: UMCG

text: public

text: human

text: Homo sapiens

text: Homo sapiens


PICH

text: UMCG

text: public

text: PICH

text: human

text: PICH

text: Homo sapiens

text: Homo sapiens

text: PICH


text: public

tag: Namespace:BAVARIAN HEALTH AND FOOD SAFETY AUTHORITY

text: not provided

characteristic: collection date

text: not provided

text: Germany

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: host health state

text: Wild Boar

characteristic: host scientific name

text: not provided

characteristic: isolate

text: Wild Boar

text: Brucella suis 01-5744

text: Brucella suis 01-5744

text: B.Suis Sample 162


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BAVARIAN HEALTH AND FOOD SAFETY AUTHORITY

characteristic: INSDC last update

text: public

text: not provided

text: not provided

text: Germany

text: not provided

text: Wild Boar

text: not provided

text: Wild Boar

text: Brucella suis 01-5744

text: Brucella suis 01-5744

text: B.Suis Sample 163


characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: not provided

text: not provided

text: Germany

text: not provided

text: Wild Boar

text: not provided

text: Wild Boar

text: Brucella suis 01-5744

text: Brucella suis 01-5744

text: B.Suis Sample 164

characteristic: title


text: public

characteristic: SRA accession

characteristic: Submitter Id

text: not provided

text: not provided

text: Germany

text: not provided

text: Wild Boar

text: not provided

text: Wild Boar

text: Brucella suis 01-5744

characteristic: organism

text: Brucella suis 01-5744

text: B.Suis Sample 165


text: public

text: not provided

characteristic: collection date

text: not provided

characteristic: description

text: Germany

text: not provided

text: Wild Boar

text: not provided

text: Wild Boar

text: Brucella suis 01-5744

text: Brucella suis 01-5744

text: B.Suis Sample 168


E-MTAB-12129:CPA- 1

text: public

text: E-MTAB-12129:CPA- 1

tag: Namespace:Max Delbrueck Center for Molecular Medicine Berlin Institute For Medical Systems Biology Free University of Berlin

unit: year

characteristic: age

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

text: Protocols: 3 normal adrenal gland samples were collected from tissue surrounding endocrine inactive adenoma bearing patients. 3 normal adrenal gland samples were collected from adrenalectomies performed on patients with renal cell carcinoma.7 cortisol producing adenoma samples were collected from patients with hypercortisolism. 5 endocrine inactive adenoma samples were collected from patients with inactive adrenal tumors. Samples were snap-frozen immediately after surgery. Nuclei were isolated from these snap frozen tissues using dounce homogenization and were purified via flow cytometry. The inDrop (1CellBio) workflow was followed to yield barcoded single-nuclei cDNA libraries. Briefly, cells were encapsulated hydrogel beads, droplet oil, RT/lysis mixture and the nuclei. The encapsulated cells were then exposed to UV light, releasing photocleavable primers from hydrogel beads. Subsequently, reverse transcription was performed and the beads filtered out. The RT product was digested using ExoI and HinFI and purified using AMpure XP beads. Then, the second strand cDNA synthesis and in vitro transcription steps were carried out. Next, the resulting RNA molecules were fragmented and a second reverse transcription step was carried out using random hexamers. The cDNA was then purified using Ampure XP beads and quantified via qPCR.

text: adult

characteristic: developmental stage

text: cortisol-producing adrenal cortex adenoma

characteristic: disease

text: CPA- 1

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: adrenal gland

characteristic: organism part

text: neoplasm

characteristic: sampling site

text: Homo sapiens

text: female

characteristic: sex

text: CPA- 1


E-MTAB-12129:CPA- 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Max Delbrueck Center for Molecular Medicine Berlin Institute For Medical Systems Biology Free University of Berlin

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-12129:CPA- 2

text: ArrayExpress

text: human

text: adult

text: CPA- 2

text: Homo sapiens

text: Homo sapiens

text: adrenal gland

text: neoplasm

text: Homo sapiens

text: female

text: CPA- 2


E-MTAB-12129:CPA- 3

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12129:CPA- 3

characteristic: Submitter Id

text: ArrayExpress

text: human

characteristic: description

text: adult

text: CPA- 3

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: adrenal gland

text: neoplasm

text: Homo sapiens

text: male

text: CPA- 3

characteristic: title


E-MTAB-12129:CPA- 4

text: public

text: E-MTAB-12129:CPA- 4

characteristic: age

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

text: adult

characteristic: developmental stage

characteristic: disease

text: CPA- 4

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: adrenal gland

text: neoplasm

text: Homo sapiens

text: female

text: CPA- 4


E-MTAB-12129:CPA- 5

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-12129:CPA- 5

text: ArrayExpress

text: human

text: adult

text: CPA- 5

text: Homo sapiens

text: Homo sapiens

text: adrenal gland

characteristic: organism part

text: neoplasm

characteristic: sampling site

text: Homo sapiens

text: female

characteristic: sex

text: CPA- 5


E-MTAB-12129:CPA- 6

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12129:CPA- 6

text: ArrayExpress

text: human

text: adult

text: CPA- 6

text: Homo sapiens

text: Homo sapiens

text: adrenal gland

text: neoplasm

text: Homo sapiens

text: female

text: CPA- 6


E-MTAB-12129:CPA- 7

text: public

text: E-MTAB-12129:CPA- 7

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: human

characteristic: description

text: adult

text: CPA- 7

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: adrenal gland

text: neoplasm

text: Homo sapiens

text: female

text: CPA- 7

characteristic: title


E-MTAB-12129:EIA- 1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-12129:EIA- 1

text: ArrayExpress

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: endocrine inactive adrenal tumor

characteristic: disease

text: EIA- 1

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: adrenal gland

text: neoplasm

text: Homo sapiens

text: female

text: EIA- 1


E-MTAB-12129:EIA- 2

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-12129:EIA- 2

text: ArrayExpress

text: human

text: adult

text: endocrine inactive adrenal tumor

text: EIA- 2

text: Homo sapiens

text: Homo sapiens

text: adrenal gland

characteristic: organism part

text: neoplasm

characteristic: sampling site

text: Homo sapiens

text: female

characteristic: sex

text: EIA- 2


E-MTAB-12129:EIA- 3

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12129:EIA- 3

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: human

text: adult

text: endocrine inactive adrenal tumor

text: EIA- 3

text: Homo sapiens

text: Homo sapiens

text: adrenal gland

text: neoplasm

text: Homo sapiens

text: female

text: EIA- 3


E-MTAB-12129:EIA- 4

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-12129:EIA- 4

text: ArrayExpress

characteristic: broker name

text: human

characteristic: description

text: adult

text: endocrine inactive adrenal tumor

text: EIA- 4

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: adrenal gland

text: neoplasm

text: Homo sapiens

text: female

text: EIA- 4

characteristic: title


E-MTAB-12129:EIA- 5

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-12129:EIA- 5

text: ArrayExpress

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: endocrine inactive adrenal tumor

characteristic: disease

text: EIA- 5

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: adrenal gland

text: neoplasm

text: Homo sapiens

text: female

text: EIA- 5


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: human

text: adult

text: endocrine inactive adrenal tumor

text: Homo sapiens

text: Homo sapiens

text: adrenal gland

characteristic: organism part

text: normal tissue adjacent to tumor

characteristic: sampling site

text: Homo sapiens

text: female

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: human

text: adult

text: endocrine inactive adrenal tumor

text: Homo sapiens

text: Homo sapiens

text: adrenal gland

text: normal tissue adjacent to tumor

text: Homo sapiens

text: male


E-MTAB-12129:NAGr- 1

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-12129:NAGr- 1

text: ArrayExpress

characteristic: broker name

text: human

characteristic: description

text: adult

text: renal cell carcinoma

text: NAGr- 1

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: adrenal gland

text: normal tissue adjacent to tumor

text: Homo sapiens

text: male

text: NAGr- 1

characteristic: title


E-MTAB-12129:NAGr- 2

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12129:NAGr- 2

text: ArrayExpress

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: renal cell carcinoma

characteristic: disease

text: NAGr- 2

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: adrenal gland

text: normal tissue adjacent to tumor

text: Homo sapiens

text: male

text: NAGr- 2


E-MTAB-12129:NAGr- 3

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC last update

text: public

text: E-MTAB-12129:NAGr- 3

text: ArrayExpress

text: human

text: adult

text: renal cell carcinoma

text: NAGr- 3

text: Homo sapiens

text: Homo sapiens

text: adrenal gland

characteristic: organism part

text: normal tissue adjacent to tumor

characteristic: sampling site

text: Homo sapiens

text: male

characteristic: sex

text: NAGr- 3


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Helsinki

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:University of Helsinki

characteristic: Submitter Id

text: urban biome (ENVO:01000249)

characteristic: broad-scale environmental context

characteristic: collection date

text: infant born by C-Section

characteristic: delivery mode

text: Stool sample Infant #I65 collected at 6 weeks of age

text: fecal material (ENVO:00002003)

characteristic: environmental medium

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

unit: week

characteristic: host age

text: feces (UBERON:0001988)

characteristic: host body product

text: male

characteristic: host sex

characteristic: host subject id

text: fecal environment (ENVO:01001029)

characteristic: local environmental context

text: human feces metagenome

characteristic: project name

text: human feces metagenome

text: Illumina

characteristic: sequencing method

text: infant stool sample


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Helsinki

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: urban biome (ENVO:01000249)

text: infant born by C-Section

text: Stool sample Infant #I246 collected at 6 weeks of age

characteristic: description

text: fecal material (ENVO:00002003)

text: Finland

text: feces (UBERON:0001988)

text: male

text: fecal environment (ENVO:01001029)

text: human feces metagenome

characteristic: organism

text: human feces metagenome

text: Illumina

text: infant stool sample

characteristic: title


text: University of Helsinki

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: urban biome (ENVO:01000249)

characteristic: broad-scale environmental context

characteristic: collection date

text: infant born by vaginal delivery

characteristic: delivery mode

text: Stool sample Infant #I1030 collected at 3 weeks of age

text: fecal material (ENVO:00002003)

characteristic: environmental medium

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: feces (UBERON:0001988)

characteristic: host body product

text: female

text: fecal environment (ENVO:01001029)

text: human feces metagenome

text: human feces metagenome

text: Illumina

text: infant stool sample


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Helsinki

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: urban biome (ENVO:01000249)

text: infant born by vaginal delivery

text: Stool sample Infant #I1019 collected at 3 weeks of age

text: fecal material (ENVO:00002003)

text: Finland

text: feces (UBERON:0001988)

text: female

characteristic: host sex

characteristic: host subject id

text: fecal environment (ENVO:01001029)

characteristic: local environmental context

text: human feces metagenome

characteristic: project name

text: human feces metagenome

text: Illumina

characteristic: sequencing method

text: infant stool sample


text: University of Helsinki

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: urban biome (ENVO:01000249)

characteristic: broad-scale environmental context

characteristic: collection date

text: infant born by vaginal delivery with intrapartum antibiotics

characteristic: delivery mode

text: Stool sample Infant #I309 collected at 18 months of age

characteristic: description

text: fecal material (ENVO:00002003)

characteristic: environmental medium

text: Finland

text: feces (UBERON:0001988)

text: female

text: fecal environment (ENVO:01001029)

text: human feces metagenome

characteristic: organism

text: human feces metagenome

text: Illumina

text: infant stool sample

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Helsinki

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: urban biome (ENVO:01000249)

text: infant born by C-Section

text: Stool sample Infant #I494 collected at 18 months of age

text: fecal material (ENVO:00002003)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: feces (UBERON:0001988)

characteristic: host body product

text: male

text: fecal environment (ENVO:01001029)

text: human feces metagenome

text: human feces metagenome

text: Illumina

text: infant stool sample


text: University of Helsinki

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: urban biome (ENVO:01000249)

characteristic: broad-scale environmental context

characteristic: collection date

text: infant born by C-Section

text: Stool sample Infant #I614 collected at 18 months of age

text: fecal material (ENVO:00002003)

text: Finland

text: feces (UBERON:0001988)

text: male

characteristic: host sex

characteristic: host subject id

text: fecal environment (ENVO:01001029)

characteristic: local environmental context

text: human feces metagenome

characteristic: project name

text: human feces metagenome

text: Illumina

characteristic: sequencing method

text: infant stool sample