characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-115631647023411:O3-B001635-1_S243

text: ArrayExpress

text: ependymal cell

text: house mouse

text: adult

text: GFP-positive

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK

characteristic: single cell well quality


status: PUBLIC

tag: attribute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Division of Cell and Developmental Biology, School of Life Sciences, University of Dundee

characteristic: Submitter Id

unit: day

characteristic: age

text: ArrayExpress

characteristic: broker name

text: ependymal cell

text: house mouse

characteristic: common name

text: Protocols: The ependymal cell layer from the thoracic spinal cord of FOXJ1-EGFP mice was microdissected and dissociated it into a cell suspension. GFP-positive DAPI-negative cells were then sorted into 384-well plates containing lysis buffer. GFP-positive DAPI-negative cells were sorted into lysis plates with a BD Influx Cell Sorter using 6 psi pressure and a 130 μm nozzle. Lysis plates were created by dispensing 0.4 μl lysis buffer (0.5 U Recombinant RNase Inhibitor (Takara Bio, 2313B), 0.0625% Triton X-100 (Sigma, 93443-100ML), 3.125 mM dNTP mix (Thermo Fisher, R0193), 3.125 μM Oligo-dT 30 VN (IDT, 5'AAGCAGTGGTATCAACGCAGAGTACT 30 VN-3') and 1:600,000 ERCC RNA spike-in mix (Thermo Fisher, 4456740)) into 384-well hard-shell PCR plates (Biorad HSP3901) using a Tempest liquid handler (Formulatrix). cDNA synthesis was performed using the Smart-seq2 protocol (Picelli et al., 2013, 2014). Briefly, 384-well plates containing single-cell lysates were thawed on ice followed by first strand synthesis. 0.6 μl of reaction mix (16.7 U/μl SMARTScribe TM Reverse Transcriptase (Takara Bio, 639538), 1.67 U/μl Recombinant RNase Inhibitor (Takara Bio, 2313B), 1.67X First-Strand Buffer (Takara Bio, 639538), 1.67 μM TSO (Exiqon, 5'-AAGCAGTGGTATCAACGCAGACTACATrGrG+G-3'), 8.33 mM DTT (Bioworld, 40420001-1), 1.67 M Betaine (Sigma, B0300-5VL), and 10 mM MgCl 2 (Sigma, M1028-10X1ML)) was added to each well using a Tempest liquid handler. Bulk wells received twice the amount of RT mix (1.2 μl). Reverse transcription was carried out by incubating wells on a ProFlex 2x384 thermal-cycler (Thermo Fisher) at 42°C for 90 min and stopped by heating at 70°C for 5 min. Subsequently, 1.6 μl of PCR mix (1.67X KAPA HiFi HotStart ReadyMix (Kapa Biosystems, KK2602), 0.17 μM IS PCR primer (IDT, 5'-AAGCAGTGGTATCAACGCAGAGT-3'), and 0.038U/μl Lambda Exonuclease (NEB, M0262L)) was added to each well with a Tempest liquid handler (Formulatrix). The amplified product was diluted with a ratio of 1 part cDNA to 10 parts 10mM Tris-HCl (Thermo Fisher, 15568025), and concentrations were measured with dye-fluorescence assay (Quant-iT dsDNA High Sensitivity kit; Thermo Fisher, Q33120) on a SpectraMax i3x microplate reader (Molecular Devices). These wells were reformatted to a new 384-well plate at a concentration of 0.3 ng/μl and final volume of 0.4 μl using an Echo 550 acoustic liquid dispenser (Labcyte). If the cell concentration was below 0.3 ng/μl, 0.4 μl of sample was transferred. Illumina sequencing libraries were prepared using the Nextera XT Library Sample Preparation kit (Illumina, FC-131-1096) (Darmanis et al., 2015; Schaum et al., 2018). Each well was mixed with 0.8 μl Nextera tagmentation DNA buffer (Illumina) and 0.4 μl Tn5 enzyme (Illumina), then tagmented at 55°C for 10 min. The reaction was stopped by adding 0.4 μl \Neutralize Tagment Buffer\ (Illumina) and spinning at room temperature (RT) in a centrifuge at 3220 xg for 5 min. Indexing PCR reactions were performed by adding 0.4 μl of 5 μM i5 indexing primer, 0.4 μl of 5 μM i7 indexing primer, and 1.2 μl of Nextera NPM mix (Illumina). PCR amplification was carried out on a ProFlex 2x384 thermal cycler using the following program: 1. 72°C for 3 minutes, 2. 95°C for 30 s, 3. 12 cycles of 95°C for 10 s, 55°C for 30 s, and 72°C for 1 min, and 4. 72°C for 5 min.

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: GFP-positive

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spinal cord

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: OK

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Division of Cell and Developmental Biology, School of Life Sciences, University of Dundee

characteristic: INSDC center name

text: public

text: ArrayExpress

text: ependymal cell

text: house mouse

text: adult

text: GFP-positive

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: not OK

characteristic: single cell well quality


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-115631647023411:O4-B001634-1_S160

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: ependymal cell

text: house mouse

characteristic: common name

text: adult

text: GFP-positive

text: Mus musculus

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK


text: public

text: E-MTAB-115631647023411:O4-B001635-1_S244

text: ArrayExpress

text: ependymal cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: GFP-positive

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spinal cord

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: OK

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: ArrayExpress

text: ependymal cell

text: house mouse

text: adult

text: GFP-positive

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK

characteristic: single cell well quality


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-115631647023411:O5-B001634-1_S161

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: ependymal cell

text: house mouse

characteristic: common name

text: adult

text: GFP-positive

text: Mus musculus

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK


text: public

text: E-MTAB-115631647023411:O5-B001635-1_S245

text: ArrayExpress

text: ependymal cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: GFP-positive

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spinal cord

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: OK

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-115631647023411:O6-B001634-1_S162

text: ArrayExpress

text: ependymal cell

text: house mouse

text: adult

text: GFP-positive

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK

characteristic: single cell well quality


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-115631647023411:O6-B001635-1_S246

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: ependymal cell

text: house mouse

characteristic: common name

text: adult

text: GFP-positive

text: Mus musculus

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK


text: public

text: E-MTAB-115631647023411:O7-B001634-1_S163

text: ArrayExpress

text: ependymal cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: GFP-positive

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spinal cord

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: OK

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-115631647023411:O7-B001635-1_S247

text: ArrayExpress

text: ependymal cell

text: house mouse

text: adult

text: GFP-positive

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK

characteristic: single cell well quality


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-115631647023411:O8-B001634-1_S164

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: ependymal cell

text: house mouse

characteristic: common name

text: adult

text: GFP-positive

text: Mus musculus

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK


text: public

text: E-MTAB-115631647023411:O8-B001635-1_S248

text: ArrayExpress

text: ependymal cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: GFP-positive

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spinal cord

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: OK

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-115631647023411:O9-B001634-1_S165

text: ArrayExpress

text: ependymal cell

text: house mouse

text: adult

text: GFP-positive

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK

characteristic: single cell well quality


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-115631647023411:O9-B001635-1_S249

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: ependymal cell

text: house mouse

characteristic: common name

text: adult

text: GFP-positive

text: Mus musculus

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK


text: public

text: E-MTAB-115631647023411:P1-B001634-1_S181

text: ArrayExpress

text: ependymal cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: GFP-positive

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spinal cord

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: not OK

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-115631647023411:P1-B001635-1_S265

text: ArrayExpress

text: ependymal cell

text: house mouse

text: adult

text: GFP-positive

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: not OK

characteristic: single cell well quality


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-115631647023411:P10-B001633-1_S106

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: ependymal cell

text: house mouse

characteristic: common name

text: adult

text: GFP-positive

text: Mus musculus

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: not OK


text: public

text: E-MTAB-115631647023411:P10-B001634-1_S190

text: ArrayExpress

text: ependymal cell

text: house mouse

text: Protocols: The ependymal cell layer from the thoracic spinal cord of FOXJ1-EGFP mice was microdissected and dissociated it into a cell suspension. GFP-positive DAPI-negative cells were then sorted into 384-well plates containing lysis buffer. GFP-positive DAPI-negative cells were sorted into lysis plates with a BD Influx Cell Sorter using 6 psi pressure and a 130 μm nozzle. Lysis plates were created by dispensing 0.4 μl lysis buffer (0.5 U Recombinant RNase Inhibitor (Takara Bio, 2313B), 0.0625% Triton X-100 (Sigma, 93443-100ML), 3.125 mM dNTP mix (Thermo Fisher, R0193), 3.125 μM Oligo-dT 30 VN (IDT, 5'AAGCAGTGGTATCAACGCAGAGTACT 30 VN-3') and 1:600,000 ERCC RNA spike-in mix (Thermo Fisher, 4456740)) into 384-well hard-shell PCR plates (Biorad HSP3901) using a Tempest liquid handler (Formulatrix). cDNA synthesis was performed using the Smart-seq2 protocol (Picelli et al., 2013, 2014). Briefly, 384-well plates containing single-cell lysates were thawed on ice followed by first strand synthesis. 0.6 μl of reaction mix (16.7 U/μl SMARTScribe TM Reverse Transcriptase (Takara Bio, 639538), 1.67 U/μl Recombinant RNase Inhibitor (Takara Bio, 2313B), 1.67X First-Strand Buffer (Takara Bio, 639538), 1.67 μM TSO (Exiqon, 5'-AAGCAGTGGTATCAACGCAGACTACATrGrG+G-3'), 8.33 mM DTT (Bioworld, 40420001-1), 1.67 M Betaine (Sigma, B0300-5VL), and 10 mM MgCl 2 (Sigma, M1028-10X1ML)) was added to each well using a Tempest liquid handler. Bulk wells received twice the amount of RT mix (1.2 μl). Reverse transcription was carried out by incubating wells on a ProFlex 2x384 thermal-cycler (Thermo Fisher) at 42°C for 90 min and stopped by heating at 70°C for 5 min. Subsequently, 1.6 μl of PCR mix (1.67X KAPA HiFi HotStart ReadyMix (Kapa Biosystems, KK2602), 0.17 μM IS PCR primer (IDT, 5'-AAGCAGTGGTATCAACGCAGAGT-3'), and 0.038U/μl Lambda Exonuclease (NEB, M0262L)) was added to each well with a Tempest liquid handler (Formulatrix). The amplified product was diluted with a ratio of 1 part cDNA to 10 parts 10mM Tris-HCl (Thermo Fisher, 15568025), and concentrations were measured with dye-fluorescence assay (Quant-iT dsDNA High Sensitivity kit; Thermo Fisher, Q33120) on a SpectraMax i3x microplate reader (Molecular Devices). These wells were reformatted to a new 384-well plate at a concentration of 0.3 ng/μl and final volume of 0.4 μl using an Echo 550 acoustic liquid dispenser (Labcyte). If the cell concentration was below 0.3 ng/μl, 0.4 μl of sample was transferred. Illumina sequencing libraries were prepared using the Nextera XT Library Sample Preparation kit (Illumina, FC-131-1096) (Darmanis et al., 2015; Schaum et al., 2018). Each well was mixed with 0.8 μl Nextera tagmentation DNA buffer (Illumina) and 0.4 μl Tn5 enzyme (Illumina), then tagmented at 55°C for 10 min. The reaction was stopped by adding 0.4 μl \Neutralize Tagment Buffer\ (Illumina) and spinning at room temperature (RT) in a centrifuge at 3220 xg for 5 min. Indexing PCR reactions were performed by adding 0.4 μl of 5 μM i5 indexing primer, 0.4 μl of 5 μM i7 indexing primer, and 1.2 μl of Nextera NPM mix (Illumina). PCR amplification was carried out on a ProFlex 2x384 thermal cycler using the following program: 1. 72°C for 3 minutes, 2. 95°C for 30 s, 3. 12 cycles of 95°C for 10 s, 55°C for 30 s, and 72°C for 1 min, and 4. 72°C for 5 min.

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: GFP-positive

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spinal cord

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: OK

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Division of Cell and Developmental Biology, School of Life Sciences, University of Dundee

characteristic: INSDC center name

text: public

text: E-MTAB-115631647023411:P10-B001635-1_S274

text: ArrayExpress

text: ependymal cell

text: house mouse

text: adult

text: GFP-positive

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK

characteristic: single cell well quality


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-115631647023411:P10-B001636-1_S58

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: ependymal cell

text: house mouse

characteristic: common name

text: adult

text: GFP-positive

text: Mus musculus

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK


text: public

text: E-MTAB-115631647023411:P11-B001633-1_S107

text: ArrayExpress

text: ependymal cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: GFP-positive

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spinal cord

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: not OK

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-115631647023411:P11-B001634-1_S191

text: ArrayExpress

text: ependymal cell

text: house mouse

text: adult

text: GFP-positive

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK

characteristic: single cell well quality


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-115631647023411:P11-B001635-1_S275

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: ependymal cell

text: house mouse

characteristic: common name

text: adult

text: GFP-positive

text: Mus musculus

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK


text: public

text: E-MTAB-115631647023411:P11-B001636-1_S59

text: ArrayExpress

text: ependymal cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: GFP-positive

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spinal cord

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: OK

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-115631647023411:P12-B001633-1_S108

text: ArrayExpress

text: ependymal cell

text: house mouse

text: adult

text: GFP-positive

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: not OK

characteristic: single cell well quality


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-115631647023411:P12-B001634-1_S192

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: ependymal cell

text: house mouse

characteristic: common name

text: adult

text: GFP-positive

text: Mus musculus

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK


text: public

text: E-MTAB-115631647023411:P12-B001635-1_S276

text: ArrayExpress

text: ependymal cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: GFP-positive

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spinal cord

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: OK

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-115631647023411:P12-B001636-1_S60

text: ArrayExpress

text: ependymal cell

text: house mouse

text: adult

text: GFP-positive

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK

characteristic: single cell well quality


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-115631647023411:P13-B001633-1_S109

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: ependymal cell

text: house mouse

characteristic: common name

text: adult

text: GFP-positive

text: Mus musculus

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: not OK


text: public

text: E-MTAB-115631647023411:P13-B001634-1_S193

text: ArrayExpress

text: ependymal cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: GFP-positive

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spinal cord

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: OK

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-115631647023411:P13-B001635-1_S277

text: ArrayExpress

text: ependymal cell

text: house mouse

text: adult

text: GFP-positive

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK

characteristic: single cell well quality


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-115631647023411:P13-B001636-1_S61

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: ependymal cell

text: house mouse

characteristic: common name

text: adult

text: GFP-positive

text: Mus musculus

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK


text: public

text: E-MTAB-115631647023411:P14-B001633-1_S110

text: ArrayExpress

text: ependymal cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: GFP-positive

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spinal cord

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: not OK

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-115631647023411:P14-B001634-1_S194

text: ArrayExpress

text: ependymal cell

text: house mouse

text: Protocols: The ependymal cell layer from the thoracic spinal cord of FOXJ1-EGFP mice was microdissected and dissociated it into a cell suspension. GFP-positive DAPI-negative cells were then sorted into 384-well plates containing lysis buffer. GFP-positive DAPI-negative cells were sorted into lysis plates with a BD Influx Cell Sorter using 6 psi pressure and a 130 μm nozzle. Lysis plates were created by dispensing 0.4 μl lysis buffer (0.5 U Recombinant RNase Inhibitor (Takara Bio, 2313B), 0.0625% Triton X-100 (Sigma, 93443-100ML), 3.125 mM dNTP mix (Thermo Fisher, R0193), 3.125 μM Oligo-dT 30 VN (IDT, 5'AAGCAGTGGTATCAACGCAGAGTACT 30 VN-3') and 1:600,000 ERCC RNA spike-in mix (Thermo Fisher, 4456740)) into 384-well hard-shell PCR plates (Biorad HSP3901) using a Tempest liquid handler (Formulatrix). cDNA synthesis was performed using the Smart-seq2 protocol (Picelli et al., 2013, 2014). Briefly, 384-well plates containing single-cell lysates were thawed on ice followed by first strand synthesis. 0.6 μl of reaction mix (16.7 U/μl SMARTScribe TM Reverse Transcriptase (Takara Bio, 639538), 1.67 U/μl Recombinant RNase Inhibitor (Takara Bio, 2313B), 1.67X First-Strand Buffer (Takara Bio, 639538), 1.67 μM TSO (Exiqon, 5'-AAGCAGTGGTATCAACGCAGACTACATrGrG+G-3'), 8.33 mM DTT (Bioworld, 40420001-1), 1.67 M Betaine (Sigma, B0300-5VL), and 10 mM MgCl 2 (Sigma, M1028-10X1ML)) was added to each well using a Tempest liquid handler. Bulk wells received twice the amount of RT mix (1.2 μl). Reverse transcription was carried out by incubating wells on a ProFlex 2x384 thermal-cycler (Thermo Fisher) at 42°C for 90 min and stopped by heating at 70°C for 5 min. Subsequently, 1.6 μl of PCR mix (1.67X KAPA HiFi HotStart ReadyMix (Kapa Biosystems, KK2602), 0.17 μM IS PCR primer (IDT, 5'-AAGCAGTGGTATCAACGCAGAGT-3'), and 0.038U/μl Lambda Exonuclease (NEB, M0262L)) was added to each well with a Tempest liquid handler (Formulatrix). The amplified product was diluted with a ratio of 1 part cDNA to 10 parts 10mM Tris-HCl (Thermo Fisher, 15568025), and concentrations were measured with dye-fluorescence assay (Quant-iT dsDNA High Sensitivity kit; Thermo Fisher, Q33120) on a SpectraMax i3x microplate reader (Molecular Devices). These wells were reformatted to a new 384-well plate at a concentration of 0.3 ng/μl and final volume of 0.4 μl using an Echo 550 acoustic liquid dispenser (Labcyte). If the cell concentration was below 0.3 ng/μl, 0.4 μl of sample was transferred. Illumina sequencing libraries were prepared using the Nextera XT Library Sample Preparation kit (Illumina, FC-131-1096) (Darmanis et al., 2015; Schaum et al., 2018). Each well was mixed with 0.8 μl Nextera tagmentation DNA buffer (Illumina) and 0.4 μl Tn5 enzyme (Illumina), then tagmented at 55°C for 10 min. The reaction was stopped by adding 0.4 μl \Neutralize Tagment Buffer\ (Illumina) and spinning at room temperature (RT) in a centrifuge at 3220 xg for 5 min. Indexing PCR reactions were performed by adding 0.4 μl of 5 μM i5 indexing primer, 0.4 μl of 5 μM i7 indexing primer, and 1.2 μl of Nextera NPM mix (Illumina). PCR amplification was carried out on a ProFlex 2x384 thermal cycler using the following program: 1. 72°C for 3 minutes, 2. 95°C for 30 s, 3. 12 cycles of 95°C for 10 s, 55°C for 30 s, and 72°C for 1 min, and 4. 72°C for 5 min.

text: adult

text: GFP-positive

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK

characteristic: single cell well quality


text: Division of Cell and Developmental Biology, School of Life Sciences, University of Dundee

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-115631647023411:P14-B001635-1_S278

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: ependymal cell

text: house mouse

characteristic: common name

text: adult

text: GFP-positive

text: Mus musculus

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK


text: public

text: E-MTAB-115631647023411:P14-B001636-1_S62

text: ArrayExpress

text: ependymal cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: GFP-positive

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spinal cord

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: OK

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-115631647023411:P15-B001633-1_S111

text: ArrayExpress

text: ependymal cell

text: house mouse

text: adult

text: GFP-positive

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: not OK

characteristic: single cell well quality


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-115631647023411:P15-B001634-1_S195

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: ependymal cell

text: house mouse

characteristic: common name

text: adult

text: GFP-positive

text: Mus musculus

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK


text: public

text: E-MTAB-115631647023411:P15-B001635-1_S279

text: ArrayExpress

text: ependymal cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: GFP-positive

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spinal cord

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: OK

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-115631647023411:P15-B001636-1_S63

text: ArrayExpress

text: ependymal cell

text: house mouse

text: adult

text: GFP-positive

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK

characteristic: single cell well quality


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-115631647023411:P16-B001633-1_S112

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: ependymal cell

text: house mouse

characteristic: common name

text: adult

text: GFP-positive

text: Mus musculus

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: not OK


text: public

text: E-MTAB-115631647023411:P16-B001634-1_S196

text: ArrayExpress

text: ependymal cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: GFP-positive

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spinal cord

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: OK

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-115631647023411:P16-B001635-1_S280

text: ArrayExpress

text: ependymal cell

text: house mouse

text: adult

text: GFP-positive

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK

characteristic: single cell well quality


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-115631647023411:P16-B001636-1_S64

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: ependymal cell

text: house mouse

characteristic: common name

text: adult

text: GFP-positive

text: Mus musculus

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK


text: public

text: E-MTAB-115631647023411:P17-B001633-1_S113

text: ArrayExpress

text: ependymal cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: GFP-positive

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spinal cord

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: not OK

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-115631647023411:P17-B001634-1_S197

text: ArrayExpress

text: ependymal cell

text: house mouse

text: adult

text: GFP-positive

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK

characteristic: single cell well quality


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-115631647023411:P17-B001635-1_S281

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: ependymal cell

text: house mouse

characteristic: common name

text: adult

text: GFP-positive

text: Mus musculus

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK


text: public

text: E-MTAB-115631647023411:P17-B001636-1_S65

text: ArrayExpress

text: ependymal cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: GFP-positive

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spinal cord

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: OK

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-115631647023411:P18-B001633-1_S114

text: ArrayExpress

text: ependymal cell

text: house mouse

text: adult

text: GFP-positive

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: not OK

characteristic: single cell well quality


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-115631647023411:P18-B001634-1_S198

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: ependymal cell

text: house mouse

characteristic: common name

text: Protocols: The ependymal cell layer from the thoracic spinal cord of FOXJ1-EGFP mice was microdissected and dissociated it into a cell suspension. GFP-positive DAPI-negative cells were then sorted into 384-well plates containing lysis buffer. GFP-positive DAPI-negative cells were sorted into lysis plates with a BD Influx Cell Sorter using 6 psi pressure and a 130 μm nozzle. Lysis plates were created by dispensing 0.4 μl lysis buffer (0.5 U Recombinant RNase Inhibitor (Takara Bio, 2313B), 0.0625% Triton X-100 (Sigma, 93443-100ML), 3.125 mM dNTP mix (Thermo Fisher, R0193), 3.125 μM Oligo-dT 30 VN (IDT, 5'AAGCAGTGGTATCAACGCAGAGTACT 30 VN-3') and 1:600,000 ERCC RNA spike-in mix (Thermo Fisher, 4456740)) into 384-well hard-shell PCR plates (Biorad HSP3901) using a Tempest liquid handler (Formulatrix). cDNA synthesis was performed using the Smart-seq2 protocol (Picelli et al., 2013, 2014). Briefly, 384-well plates containing single-cell lysates were thawed on ice followed by first strand synthesis. 0.6 μl of reaction mix (16.7 U/μl SMARTScribe TM Reverse Transcriptase (Takara Bio, 639538), 1.67 U/μl Recombinant RNase Inhibitor (Takara Bio, 2313B), 1.67X First-Strand Buffer (Takara Bio, 639538), 1.67 μM TSO (Exiqon, 5'-AAGCAGTGGTATCAACGCAGACTACATrGrG+G-3'), 8.33 mM DTT (Bioworld, 40420001-1), 1.67 M Betaine (Sigma, B0300-5VL), and 10 mM MgCl 2 (Sigma, M1028-10X1ML)) was added to each well using a Tempest liquid handler. Bulk wells received twice the amount of RT mix (1.2 μl). Reverse transcription was carried out by incubating wells on a ProFlex 2x384 thermal-cycler (Thermo Fisher) at 42°C for 90 min and stopped by heating at 70°C for 5 min. Subsequently, 1.6 μl of PCR mix (1.67X KAPA HiFi HotStart ReadyMix (Kapa Biosystems, KK2602), 0.17 μM IS PCR primer (IDT, 5'-AAGCAGTGGTATCAACGCAGAGT-3'), and 0.038U/μl Lambda Exonuclease (NEB, M0262L)) was added to each well with a Tempest liquid handler (Formulatrix). The amplified product was diluted with a ratio of 1 part cDNA to 10 parts 10mM Tris-HCl (Thermo Fisher, 15568025), and concentrations were measured with dye-fluorescence assay (Quant-iT dsDNA High Sensitivity kit; Thermo Fisher, Q33120) on a SpectraMax i3x microplate reader (Molecular Devices). These wells were reformatted to a new 384-well plate at a concentration of 0.3 ng/μl and final volume of 0.4 μl using an Echo 550 acoustic liquid dispenser (Labcyte). If the cell concentration was below 0.3 ng/μl, 0.4 μl of sample was transferred. Illumina sequencing libraries were prepared using the Nextera XT Library Sample Preparation kit (Illumina, FC-131-1096) (Darmanis et al., 2015; Schaum et al., 2018). Each well was mixed with 0.8 μl Nextera tagmentation DNA buffer (Illumina) and 0.4 μl Tn5 enzyme (Illumina), then tagmented at 55°C for 10 min. The reaction was stopped by adding 0.4 μl \Neutralize Tagment Buffer\ (Illumina) and spinning at room temperature (RT) in a centrifuge at 3220 xg for 5 min. Indexing PCR reactions were performed by adding 0.4 μl of 5 μM i5 indexing primer, 0.4 μl of 5 μM i7 indexing primer, and 1.2 μl of Nextera NPM mix (Illumina). PCR amplification was carried out on a ProFlex 2x384 thermal cycler using the following program: 1. 72°C for 3 minutes, 2. 95°C for 30 s, 3. 12 cycles of 95°C for 10 s, 55°C for 30 s, and 72°C for 1 min, and 4. 72°C for 5 min.

text: adult

text: GFP-positive

text: Mus musculus

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK


text: Division of Cell and Developmental Biology, School of Life Sciences, University of Dundee

text: public

text: E-MTAB-115631647023411:P18-B001635-1_S282

text: ArrayExpress

text: ependymal cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: GFP-positive

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spinal cord

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: OK

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-115631647023411:P18-B001636-1_S66

text: ArrayExpress

text: ependymal cell

text: house mouse

text: adult

text: GFP-positive

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK

characteristic: single cell well quality


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-115631647023411:P19-B001633-1_S115

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: ependymal cell

text: house mouse

characteristic: common name

text: adult

text: GFP-positive

text: Mus musculus

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: not OK


text: public

text: E-MTAB-115631647023411:P19-B001634-1_S199

text: ArrayExpress

text: ependymal cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: GFP-positive

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spinal cord

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: OK

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-115631647023411:P19-B001635-1_S283

text: ArrayExpress

text: ependymal cell

text: house mouse

text: adult

text: GFP-positive

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK

characteristic: single cell well quality


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-115631647023411:P19-B001636-1_S67

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: ependymal cell

text: house mouse

characteristic: common name

text: adult

text: GFP-positive

text: Mus musculus

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK


text: public

text: ArrayExpress

text: ependymal cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: GFP-positive

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spinal cord

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: not OK

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-115631647023411:P2-B001634-1_S182

text: ArrayExpress

text: ependymal cell

text: house mouse

text: adult

text: GFP-positive

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: not OK

characteristic: single cell well quality


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-115631647023411:P2-B001635-1_S266

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: ependymal cell

text: house mouse

characteristic: common name

text: adult

text: GFP-positive

text: Mus musculus

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: not OK


text: public

text: E-MTAB-115631647023411:P20-B001633-1_S116

text: ArrayExpress

text: ependymal cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: GFP-positive

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spinal cord

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: not OK

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-115631647023411:P20-B001634-1_S200

text: ArrayExpress

text: ependymal cell

text: house mouse

text: adult

text: GFP-positive

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK

characteristic: single cell well quality


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-115631647023411:P20-B001635-1_S284

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: ependymal cell

text: house mouse

characteristic: common name

text: adult

text: GFP-positive

text: Mus musculus

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK


text: public

text: E-MTAB-115631647023411:P20-B001636-1_S68

text: ArrayExpress

text: ependymal cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: GFP-positive

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spinal cord

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: OK

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-115631647023411:P21-B001633-1_S117

text: ArrayExpress

text: ependymal cell

text: house mouse

text: adult

text: GFP-positive

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: not OK

characteristic: single cell well quality


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-115631647023411:P21-B001634-1_S201

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: ependymal cell

text: house mouse

characteristic: common name

text: adult

text: GFP-positive

text: Mus musculus

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK


text: public

text: E-MTAB-115631647023411:P21-B001635-1_S285

text: ArrayExpress

text: ependymal cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: GFP-positive

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spinal cord

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: OK

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-115631647023411:P21-B001636-1_S69

text: ArrayExpress

text: ependymal cell

text: house mouse

text: Protocols: The ependymal cell layer from the thoracic spinal cord of FOXJ1-EGFP mice was microdissected and dissociated it into a cell suspension. GFP-positive DAPI-negative cells were then sorted into 384-well plates containing lysis buffer. GFP-positive DAPI-negative cells were sorted into lysis plates with a BD Influx Cell Sorter using 6 psi pressure and a 130 μm nozzle. Lysis plates were created by dispensing 0.4 μl lysis buffer (0.5 U Recombinant RNase Inhibitor (Takara Bio, 2313B), 0.0625% Triton X-100 (Sigma, 93443-100ML), 3.125 mM dNTP mix (Thermo Fisher, R0193), 3.125 μM Oligo-dT 30 VN (IDT, 5'AAGCAGTGGTATCAACGCAGAGTACT 30 VN-3') and 1:600,000 ERCC RNA spike-in mix (Thermo Fisher, 4456740)) into 384-well hard-shell PCR plates (Biorad HSP3901) using a Tempest liquid handler (Formulatrix). cDNA synthesis was performed using the Smart-seq2 protocol (Picelli et al., 2013, 2014). Briefly, 384-well plates containing single-cell lysates were thawed on ice followed by first strand synthesis. 0.6 μl of reaction mix (16.7 U/μl SMARTScribe TM Reverse Transcriptase (Takara Bio, 639538), 1.67 U/μl Recombinant RNase Inhibitor (Takara Bio, 2313B), 1.67X First-Strand Buffer (Takara Bio, 639538), 1.67 μM TSO (Exiqon, 5'-AAGCAGTGGTATCAACGCAGACTACATrGrG+G-3'), 8.33 mM DTT (Bioworld, 40420001-1), 1.67 M Betaine (Sigma, B0300-5VL), and 10 mM MgCl 2 (Sigma, M1028-10X1ML)) was added to each well using a Tempest liquid handler. Bulk wells received twice the amount of RT mix (1.2 μl). Reverse transcription was carried out by incubating wells on a ProFlex 2x384 thermal-cycler (Thermo Fisher) at 42°C for 90 min and stopped by heating at 70°C for 5 min. Subsequently, 1.6 μl of PCR mix (1.67X KAPA HiFi HotStart ReadyMix (Kapa Biosystems, KK2602), 0.17 μM IS PCR primer (IDT, 5'-AAGCAGTGGTATCAACGCAGAGT-3'), and 0.038U/μl Lambda Exonuclease (NEB, M0262L)) was added to each well with a Tempest liquid handler (Formulatrix). The amplified product was diluted with a ratio of 1 part cDNA to 10 parts 10mM Tris-HCl (Thermo Fisher, 15568025), and concentrations were measured with dye-fluorescence assay (Quant-iT dsDNA High Sensitivity kit; Thermo Fisher, Q33120) on a SpectraMax i3x microplate reader (Molecular Devices). These wells were reformatted to a new 384-well plate at a concentration of 0.3 ng/μl and final volume of 0.4 μl using an Echo 550 acoustic liquid dispenser (Labcyte). If the cell concentration was below 0.3 ng/μl, 0.4 μl of sample was transferred. Illumina sequencing libraries were prepared using the Nextera XT Library Sample Preparation kit (Illumina, FC-131-1096) (Darmanis et al., 2015; Schaum et al., 2018). Each well was mixed with 0.8 μl Nextera tagmentation DNA buffer (Illumina) and 0.4 μl Tn5 enzyme (Illumina), then tagmented at 55°C for 10 min. The reaction was stopped by adding 0.4 μl \Neutralize Tagment Buffer\ (Illumina) and spinning at room temperature (RT) in a centrifuge at 3220 xg for 5 min. Indexing PCR reactions were performed by adding 0.4 μl of 5 μM i5 indexing primer, 0.4 μl of 5 μM i7 indexing primer, and 1.2 μl of Nextera NPM mix (Illumina). PCR amplification was carried out on a ProFlex 2x384 thermal cycler using the following program: 1. 72°C for 3 minutes, 2. 95°C for 30 s, 3. 12 cycles of 95°C for 10 s, 55°C for 30 s, and 72°C for 1 min, and 4. 72°C for 5 min.

text: adult

text: GFP-positive

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK

characteristic: single cell well quality


text: Division of Cell and Developmental Biology, School of Life Sciences, University of Dundee

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-115631647023411:P22-B001633-1_S118

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: ependymal cell

text: house mouse

characteristic: common name

text: adult

text: GFP-positive

text: Mus musculus

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: not OK


text: public

text: E-MTAB-115631647023411:P22-B001634-1_S202

text: ArrayExpress

text: ependymal cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: GFP-positive

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spinal cord

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: OK

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-115631647023411:P22-B001635-1_S286

text: ArrayExpress

text: ependymal cell

text: house mouse

text: adult

text: GFP-positive

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK

characteristic: single cell well quality


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-115631647023411:P22-B001636-1_S70

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: ependymal cell

text: house mouse

characteristic: common name

text: adult

text: GFP-positive

text: Mus musculus

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK


text: public

text: E-MTAB-115631647023411:P23-B001633-1_S119

text: ArrayExpress

text: ependymal cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: GFP-positive

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spinal cord

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: not OK

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-115631647023411:P23-B001634-1_S203

text: ArrayExpress

text: ependymal cell

text: house mouse

text: adult

text: GFP-positive

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK

characteristic: single cell well quality


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-115631647023411:P23-B001635-1_S287

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: ependymal cell

text: house mouse

characteristic: common name

text: adult

text: GFP-positive

text: Mus musculus

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK


text: public

text: E-MTAB-115631647023411:P23-B001636-1_S71

text: ArrayExpress

text: ependymal cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: GFP-positive

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spinal cord

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: OK

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-115631647023411:P24-B001633-1_S73

text: ArrayExpress

text: ependymal cell

text: house mouse

text: adult

text: GFP-positive

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: not OK

characteristic: single cell well quality


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-115631647023411:P24-B001634-1_S74

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: ependymal cell

text: house mouse

characteristic: common name

text: adult

text: GFP-positive

text: Mus musculus

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK


text: public

text: E-MTAB-115631647023411:P24-B001635-1_S75

text: ArrayExpress

text: ependymal cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: GFP-positive

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spinal cord

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: OK

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-115631647023411:P24-B001636-1_S76

text: ArrayExpress

text: ependymal cell

text: house mouse

text: adult

text: GFP-positive

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK

characteristic: single cell well quality


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: ependymal cell

text: house mouse

characteristic: common name

text: adult

text: GFP-positive

text: Mus musculus

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: not OK


text: public

text: E-MTAB-115631647023411:P3-B001634-1_S183

text: ArrayExpress

text: ependymal cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: GFP-positive

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spinal cord

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: not OK

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-115631647023411:P3-B001635-1_S267

text: ArrayExpress

text: ependymal cell

text: house mouse

text: adult

text: GFP-positive

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: not OK

characteristic: single cell well quality


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-115631647023411:P4-B001633-1_S100

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: ependymal cell

text: house mouse

characteristic: common name

text: adult

text: GFP-positive

text: Mus musculus

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: not OK


text: public

text: E-MTAB-115631647023411:P4-B001634-1_S184

text: ArrayExpress

text: ependymal cell

text: house mouse

text: Protocols: The ependymal cell layer from the thoracic spinal cord of FOXJ1-EGFP mice was microdissected and dissociated it into a cell suspension. GFP-positive DAPI-negative cells were then sorted into 384-well plates containing lysis buffer. GFP-positive DAPI-negative cells were sorted into lysis plates with a BD Influx Cell Sorter using 6 psi pressure and a 130 μm nozzle. Lysis plates were created by dispensing 0.4 μl lysis buffer (0.5 U Recombinant RNase Inhibitor (Takara Bio, 2313B), 0.0625% Triton X-100 (Sigma, 93443-100ML), 3.125 mM dNTP mix (Thermo Fisher, R0193), 3.125 μM Oligo-dT 30 VN (IDT, 5'AAGCAGTGGTATCAACGCAGAGTACT 30 VN-3') and 1:600,000 ERCC RNA spike-in mix (Thermo Fisher, 4456740)) into 384-well hard-shell PCR plates (Biorad HSP3901) using a Tempest liquid handler (Formulatrix). cDNA synthesis was performed using the Smart-seq2 protocol (Picelli et al., 2013, 2014). Briefly, 384-well plates containing single-cell lysates were thawed on ice followed by first strand synthesis. 0.6 μl of reaction mix (16.7 U/μl SMARTScribe TM Reverse Transcriptase (Takara Bio, 639538), 1.67 U/μl Recombinant RNase Inhibitor (Takara Bio, 2313B), 1.67X First-Strand Buffer (Takara Bio, 639538), 1.67 μM TSO (Exiqon, 5'-AAGCAGTGGTATCAACGCAGACTACATrGrG+G-3'), 8.33 mM DTT (Bioworld, 40420001-1), 1.67 M Betaine (Sigma, B0300-5VL), and 10 mM MgCl 2 (Sigma, M1028-10X1ML)) was added to each well using a Tempest liquid handler. Bulk wells received twice the amount of RT mix (1.2 μl). Reverse transcription was carried out by incubating wells on a ProFlex 2x384 thermal-cycler (Thermo Fisher) at 42°C for 90 min and stopped by heating at 70°C for 5 min. Subsequently, 1.6 μl of PCR mix (1.67X KAPA HiFi HotStart ReadyMix (Kapa Biosystems, KK2602), 0.17 μM IS PCR primer (IDT, 5'-AAGCAGTGGTATCAACGCAGAGT-3'), and 0.038U/μl Lambda Exonuclease (NEB, M0262L)) was added to each well with a Tempest liquid handler (Formulatrix). The amplified product was diluted with a ratio of 1 part cDNA to 10 parts 10mM Tris-HCl (Thermo Fisher, 15568025), and concentrations were measured with dye-fluorescence assay (Quant-iT dsDNA High Sensitivity kit; Thermo Fisher, Q33120) on a SpectraMax i3x microplate reader (Molecular Devices). These wells were reformatted to a new 384-well plate at a concentration of 0.3 ng/μl and final volume of 0.4 μl using an Echo 550 acoustic liquid dispenser (Labcyte). If the cell concentration was below 0.3 ng/μl, 0.4 μl of sample was transferred. Illumina sequencing libraries were prepared using the Nextera XT Library Sample Preparation kit (Illumina, FC-131-1096) (Darmanis et al., 2015; Schaum et al., 2018). Each well was mixed with 0.8 μl Nextera tagmentation DNA buffer (Illumina) and 0.4 μl Tn5 enzyme (Illumina), then tagmented at 55°C for 10 min. The reaction was stopped by adding 0.4 μl \Neutralize Tagment Buffer\ (Illumina) and spinning at room temperature (RT) in a centrifuge at 3220 xg for 5 min. Indexing PCR reactions were performed by adding 0.4 μl of 5 μM i5 indexing primer, 0.4 μl of 5 μM i7 indexing primer, and 1.2 μl of Nextera NPM mix (Illumina). PCR amplification was carried out on a ProFlex 2x384 thermal cycler using the following program: 1. 72°C for 3 minutes, 2. 95°C for 30 s, 3. 12 cycles of 95°C for 10 s, 55°C for 30 s, and 72°C for 1 min, and 4. 72°C for 5 min.

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: GFP-positive

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spinal cord

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: multiple

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Division of Cell and Developmental Biology, School of Life Sciences, University of Dundee

characteristic: INSDC center name

text: public

text: E-MTAB-115631647023411:P4-B001635-1_S268

text: ArrayExpress

text: ependymal cell

text: house mouse

text: adult

text: GFP-positive

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: multiple

characteristic: single cell well quality


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: ependymal cell

text: house mouse

characteristic: common name

text: adult

text: GFP-positive

text: Mus musculus

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: multiple


text: public

text: E-MTAB-115631647023411:P5-B001633-1_S101

text: ArrayExpress

text: ependymal cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: GFP-positive

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spinal cord

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: not OK

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-115631647023411:P5-B001634-1_S185

text: ArrayExpress

text: ependymal cell

text: house mouse

text: adult

text: GFP-positive

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK

characteristic: single cell well quality


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-115631647023411:P5-B001635-1_S269

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: ependymal cell

text: house mouse

characteristic: common name

text: adult

text: GFP-positive

text: Mus musculus

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK


text: public

text: ArrayExpress

text: ependymal cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: GFP-positive

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spinal cord

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: OK

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-115631647023411:P6-B001633-1_S102

text: ArrayExpress

text: ependymal cell

text: house mouse

text: adult

text: GFP-positive

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: not OK

characteristic: single cell well quality


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-115631647023411:P6-B001634-1_S186

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: ependymal cell

text: house mouse

characteristic: common name

text: adult

text: GFP-positive

text: Mus musculus

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK


text: public

text: E-MTAB-115631647023411:P6-B001635-1_S270

text: ArrayExpress

text: ependymal cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: GFP-positive

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spinal cord

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: OK

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-115631647023411:P7-B001633-1_S103

text: ArrayExpress

text: ependymal cell

text: house mouse

text: adult

text: GFP-positive

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: not OK

characteristic: single cell well quality


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-115631647023411:P7-B001634-1_S187

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: ependymal cell

text: house mouse

characteristic: common name

text: adult

text: GFP-positive

text: Mus musculus

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK


text: public

text: E-MTAB-115631647023411:P7-B001635-1_S271

text: ArrayExpress

text: ependymal cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: GFP-positive

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spinal cord

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: OK

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-115631647023411:P8-B001633-1_S104

text: ArrayExpress

text: ependymal cell

text: house mouse

text: adult

text: GFP-positive

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: not OK

characteristic: single cell well quality


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-115631647023411:P8-B001634-1_S188

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: ependymal cell

text: house mouse

characteristic: common name

text: adult

text: GFP-positive

text: Mus musculus

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK


text: public

text: E-MTAB-115631647023411:P8-B001635-1_S272

text: ArrayExpress

text: ependymal cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: GFP-positive

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spinal cord

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: OK

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-115631647023411:P9-B001633-1_S105

text: ArrayExpress

text: ependymal cell

text: house mouse

text: adult

text: GFP-positive

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: not OK

characteristic: single cell well quality


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-115631647023411:P9-B001634-1_S189

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: ependymal cell

text: house mouse

characteristic: common name

text: adult

text: GFP-positive

text: Mus musculus

text: Mus musculus

text: spinal cord

text: Mus musculus

text: female

text: OK


text: public

text: E-MTAB-115631647023411:P9-B001635-1_S273

text: ArrayExpress

text: ependymal cell

text: house mouse

text: Protocols: The ependymal cell layer from the thoracic spinal cord of FOXJ1-EGFP mice was microdissected and dissociated it into a cell suspension. GFP-positive DAPI-negative cells were then sorted into 384-well plates containing lysis buffer. GFP-positive DAPI-negative cells were sorted into lysis plates with a BD Influx Cell Sorter using 6 psi pressure and a 130 μm nozzle. Lysis plates were created by dispensing 0.4 μl lysis buffer (0.5 U Recombinant RNase Inhibitor (Takara Bio, 2313B), 0.0625% Triton X-100 (Sigma, 93443-100ML), 3.125 mM dNTP mix (Thermo Fisher, R0193), 3.125 μM Oligo-dT 30 VN (IDT, 5'AAGCAGTGGTATCAACGCAGAGTACT 30 VN-3') and 1:600,000 ERCC RNA spike-in mix (Thermo Fisher, 4456740)) into 384-well hard-shell PCR plates (Biorad HSP3901) using a Tempest liquid handler (Formulatrix). cDNA synthesis was performed using the Smart-seq2 protocol (Picelli et al., 2013, 2014). Briefly, 384-well plates containing single-cell lysates were thawed on ice followed by first strand synthesis. 0.6 μl of reaction mix (16.7 U/μl SMARTScribe TM Reverse Transcriptase (Takara Bio, 639538), 1.67 U/μl Recombinant RNase Inhibitor (Takara Bio, 2313B), 1.67X First-Strand Buffer (Takara Bio, 639538), 1.67 μM TSO (Exiqon, 5'-AAGCAGTGGTATCAACGCAGACTACATrGrG+G-3'), 8.33 mM DTT (Bioworld, 40420001-1), 1.67 M Betaine (Sigma, B0300-5VL), and 10 mM MgCl 2 (Sigma, M1028-10X1ML)) was added to each well using a Tempest liquid handler. Bulk wells received twice the amount of RT mix (1.2 μl). Reverse transcription was carried out by incubating wells on a ProFlex 2x384 thermal-cycler (Thermo Fisher) at 42°C for 90 min and stopped by heating at 70°C for 5 min. Subsequently, 1.6 μl of PCR mix (1.67X KAPA HiFi HotStart ReadyMix (Kapa Biosystems, KK2602), 0.17 μM IS PCR primer (IDT, 5'-AAGCAGTGGTATCAACGCAGAGT-3'), and 0.038U/μl Lambda Exonuclease (NEB, M0262L)) was added to each well with a Tempest liquid handler (Formulatrix). The amplified product was diluted with a ratio of 1 part cDNA to 10 parts 10mM Tris-HCl (Thermo Fisher, 15568025), and concentrations were measured with dye-fluorescence assay (Quant-iT dsDNA High Sensitivity kit; Thermo Fisher, Q33120) on a SpectraMax i3x microplate reader (Molecular Devices). These wells were reformatted to a new 384-well plate at a concentration of 0.3 ng/μl and final volume of 0.4 μl using an Echo 550 acoustic liquid dispenser (Labcyte). If the cell concentration was below 0.3 ng/μl, 0.4 μl of sample was transferred. Illumina sequencing libraries were prepared using the Nextera XT Library Sample Preparation kit (Illumina, FC-131-1096) (Darmanis et al., 2015; Schaum et al., 2018). Each well was mixed with 0.8 μl Nextera tagmentation DNA buffer (Illumina) and 0.4 μl Tn5 enzyme (Illumina), then tagmented at 55°C for 10 min. The reaction was stopped by adding 0.4 μl \Neutralize Tagment Buffer\ (Illumina) and spinning at room temperature (RT) in a centrifuge at 3220 xg for 5 min. Indexing PCR reactions were performed by adding 0.4 μl of 5 μM i5 indexing primer, 0.4 μl of 5 μM i7 indexing primer, and 1.2 μl of Nextera NPM mix (Illumina). PCR amplification was carried out on a ProFlex 2x384 thermal cycler using the following program: 1. 72°C for 3 minutes, 2. 95°C for 30 s, 3. 12 cycles of 95°C for 10 s, 55°C for 30 s, and 72°C for 1 min, and 4. 72°C for 5 min.

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: GFP-positive

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spinal cord

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: OK

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Marine College, Shandong University

characteristic: INSDC center name

text: public

tag: Namespace:Marine College, Shandong University

characteristic: collection date

text: Macroalgea were then put in sterile seawater and remaining bacteria were removed by ultrasonication (2x 60s, 50-60 kHz). Suspensions with removed bacteria of each sample were subsequently pooled with the previously obtained washing suspensions. Per sample, nearly 60 L of washing suspension was then filtered through 0.2 µm pore size polycarbonate membrane filters (Millipore). DNA was extracted from these filters, frozen at -80°C.

text: marine biome [ENVO:00000447]

characteristic: environment (biome)

text: microbial feature [ENVO:00002150]

characteristic: environment (feature)

text: Macroalgae

characteristic: environment (material)

text: China

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: marine metagenome

characteristic: organism

text: plant-associated

characteristic: plant-associated environmental package

text: Metagenomes of marine macroalgae, surrounding seawater and surrounding sediments

characteristic: project name

text: marine metagenome

text: Illumina Nova-PE150

characteristic: sequencing method


E-MTAB-11305:iCRISPR control EB 18h

characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11305:iCRISPR control EB 18h

tag: Namespace:The Gurdon Institute, University of Cambridge

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: H9 iCRISPR WT

text: primordial germ cell

text: human

characteristic: common name

text: Protocols: Embryoid bodies were dissociated using TrypLE, neutralised using MEF media and sorted to remove doublets and debries using a SONY SH800 sorter For the TFAP2A knockout and parental control line E-MTAB-11305 we used HUES9 iCRISPR and H9-TFAP2A lines ESC (https://www.pnas.org/doi/10.1073/pnas.1708341114#sec-3) which are derived from H9 cell line https://www.wicell.org/home/stem-cells/catalog-of-stem-cell-lines/wa09.cmsx. Embryoid bodies were aggregated from PreME cells and treated with BMP to induce PGCLC fate according to Kobayashi et al 2017. PreME/ME protocols were optimised based on previous conditions. For the basal medium, we used aRB27 medium which is composed of Advanced RPMI 1640 Medium (Thermo Fisher Scientific) supplemented with 1% B27 supplement (Thermo Fisher Scientific), 0.1 mM NEAA, 100 U/ml Penicillin- 0.1 mg/ml Streptomycin, 2 mM L-Glutamine. For PreME/ME induction, trypsinized Conv-hPSC were seeded on vitronectin-coated dish at 200,000 cells/well in 12 well plate and cultured in PreME/ME induction medium for 6-36 h as indicated in the figures. PreME/ME induction medium was composed of aRB27 medium supplemental with 100 ng/ml Activin-A (SCI), 3 ŒºM GSK3i and 10 ŒºM of ROCKi. Single Cell 3' 10X V3 Single Cell 3' 10X V3

characteristic: description

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: embryoid body

characteristic: growth condition

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: iCRISPR control EB 18h

characteristic: title


E-MTAB-11305:iCRISPR control EB d4

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Gurdon Institute, University of Cambridge

characteristic: INSDC center name

text: public

text: E-MTAB-11305:iCRISPR control EB d4

text: ArrayExpress

text: H9 iCRISPR WT

text: primordial germ cell

text: human

text: embryo

text: normal

text: wild type genotype

text: embryoid body

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

text: embryonic stem cell

text: Homo sapiens

text: iCRISPR control EB d4


E-MTAB-11305:TFAP2A KO EB 18h

characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11305:TFAP2A KO EB 18h

text: ArrayExpress

text: H9 TFAP2A KO

text: primordial germ cell

text: human

text: embryo

text: normal

text: Crspr/Cas9 mediated TFAP2A knockout

text: embryoid body

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: embryonic stem cell

text: Homo sapiens

text: TFAP2A KO EB 18h


E-MTAB-11305:TFAP2A KO EB d4

text: public

text: E-MTAB-11305:TFAP2A KO EB d4

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: H9 TFAP2A KO

text: primordial germ cell

text: human

characteristic: common name

characteristic: description

text: embryo

characteristic: developmental stage

text: normal

text: Crspr/Cas9 mediated TFAP2A knockout

text: embryoid body

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: embryonic stem cell

text: Homo sapiens

text: TFAP2A KO EB d4


text: Helmholtz Pioneer Campus

text: public

tag: Namespace:Helmholtz Pioneer Campus

unit: year

characteristic: age

characteristic: batch

text: ArrayExpress

text: hepatocyte

text: human

text: Protocols: Cryopreserved primary human hepatocytes were purchased from Lonza. Primary human hepatocytes were plated for 6 hours and incubated for 66 hours with either vehicle (DMSO) or a 5-probe cocktail (Sanofi-Aventis). Hepatic steatosis was modeled by incubating the cells with free fatty acid (FFA) corresponding to 200 µM mixture of a 2:1 ratio of oleic acid to palmitic acid for a total of72 hours. Four different conditions have thereby been studied: i) Vehicle (DMSO 0.5% v/v); ii) Cocktail (66h five-drug cocktail incubation); iii) FFA (2:1 oleic:palmitic acid) and iv) FFA+Cocktail (Combination of FFA incubation and 5-drug cocktail). The components of the cocktail were dissolved in DMSO to a final concentration of: 50 mM of Caffeine; 30 mM of Metoprolol;n25 mM of Omeprazole; 20 mM of S-Warfarin and 5.82 mM of Midazolam Single-cell encapsulation and lysis in oil droplets was performed following the Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 User Guide Libraries were prepared following the Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 User Guide.

text: adult

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: liver

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex


Pge smallRNA

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF OSLO

characteristic: INSDC center name

text: public

text: Pge smallRNA

tag: Namespace:UNIVERSITY OF OSLO

text: mixed

text: small RNAs from Pirum gemmata

text: mixed

text: Pirum gemmata

characteristic: organism

text: Pirum gemmata

text: Pge smallRNA

characteristic: title


Sta smallRNA

text: UNIVERSITY OF OSLO

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Sta smallRNA

characteristic: Submitter Id

text: mixed

text: small RNAs from Sphaeroforma tapetis

text: mixed

text: Sphaeroforma tapetis

text: Sphaeroforma tapetis

text: Sta smallRNA


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:202-11666

tag: Namespace:University of Toronto Mississauga

text: Ancestor

characteristic: description

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:202-11667

text: Ancestor

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:202-11668

text: Ancestor

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:202-11669

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:202-11670

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:202-11671

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:202-11672

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:202-11673

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:203-11674

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:203-11675

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:203-11676

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:203-11677

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:203-11678

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:203-11679

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:203-11680

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:203-11681

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:203-11682

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:203-11683

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:203-11684

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:203-11685

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:203-11686

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:203-11687

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:203-11688

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:203-11689

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:203-11690

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:203-11691

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:203-11692

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:203-11693

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:203-11694

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:203-11695

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:203-11696

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:203-11697

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:203-11698

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:203-11699

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:203-11700

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:203-11701

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:203-11702

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:203-11703

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:203-11704

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:203-11705

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:203-11706

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:204-11707

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:204-11708

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:204-11709

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:204-11710

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:204-11711

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:204-11712

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:204-11713

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:204-11714

text: Ancestor

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:204-11715

text: Ancestor

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:204-11716

text: Ancestor

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:204-11717

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:204-11718

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:204-11719

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:204-11720

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:204-11721

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:204-11722

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:204-11723

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:204-11724

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:204-11725

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:204-11726

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

text: ena-SAMPLE-TAB-16-03-2022-01:33:32:204-11727

text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: University of Toronto Mississauga

text: public

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text: MA line

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii