characteristic: BACPHLIP temperate score

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

text: Reads (ERR7803603) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (NA) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15.

characteristic: MAG coverage software

characteristic: Number of BLAST hits supporting host taxonomy

characteristic: Number of CRISPR spacer hits supporting host taxonomy

characteristic: SRA accession

text: Hadza_MoBio_hadza-A-D_C_6_1128__k127_1503354__flag_3__multi_388.0000__len_190212

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: CheckV; DTR (high-confidence)

characteristic: completeness approach

characteristic: completeness score

text: CheckV;0.8.1;end-to-end, database v1.0

characteristic: completeness software

text: This sample represents an uncultured crAss-like viruses virus assembled from ERR7803603.

characteristic: description

text: independent sequence (UViG)

characteristic: detection type

text: terrestrial biome

characteristic: environment (biome)

text: human-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: Tanzania

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: CRISPR spacer matches (PILER-CR;v1.06 or CRT;v1.2; blastn;2.10.1+ -dust no -word_size 18, allowing <=1 gap or mismatch over 95% of spacer length) and >=1kb+>=96% identity BLAST hits (hs-blastn max_target_seqs 25000 -perc_identity 96 -evalue 1e-180) were used to identify connections between each viral contig and all non-viral contigs in the UHGG v1 (286,997 genomes) and this study (54,779 genomes). The predicted host for each viral contig is the lowest taxonomic rank with >70% agreement among all CRISPR+BLAST connections, as described previously (https://doi.org/10.1038/s41564-021-00928-6).

characteristic: host prediction approach

text: uncultivated viral genomes

characteristic: investigation type

text: human feces

text: human gut metagenome

characteristic: metagenomic source

characteristic: nucleic acid extraction

characteristic: number of standard tRNAs extracted

characteristic: number of unique standard tRNAs extracted

text: uncultured podovirus

characteristic: organism

characteristic: percent of BLAST hits supporting viral taxonomy

text: non-segmented

characteristic: predicted genome structure

text: dsDNA

characteristic: predicted genome type

text: Human gut UViGs

characteristic: project name

characteristic: reference database(s)

text: uncultured podovirus

text: Illumina NovaSeq 6000

text: diamond;2.0.9;--sensitive -k 10 --query-cover 50 --subject-cover 50 --evalue 1e-5

text: metagenome (not viral targeted)

text: d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella;unk;unk

text: 95% ANI;85% AF;greedy, centroid-based

characteristic: vOTU sequence comparison approach

text: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; crAss-like viruses


status: PUBLIC

tag: attribute

characteristic: BACPHLIP temperate score

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

characteristic: MAG coverage software

characteristic: Number of BLAST hits supporting host taxonomy

characteristic: Number of CRISPR spacer hits supporting host taxonomy

characteristic: SRA accession

text: Hadza_MoBio_hadza-A-D_C_6_1128__k127_1687331__flag_3__multi_106.0000__len_53017

tag: Namespace:Stanford University School of Medicine

characteristic: Submitter Id

text: Multiple fragments where gaps span repetitive regions. Presence of the 23S, 16S and 5S rRNA genes and at least 18 tRNAs.

characteristic: assembly quality

characteristic: assembly software

text: CheckV; DTR (high-confidence)

characteristic: completeness approach

characteristic: completeness score

text: CheckV;0.8.1;end-to-end, database v1.0

characteristic: completeness software

text: This sample represents an uncultured Siphoviridae virus assembled from ERR7803603.

characteristic: description

text: independent sequence (UViG)

characteristic: detection type

text: terrestrial biome

characteristic: environment (biome)

text: human-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

characteristic: feature prediction

text: Tanzania

characteristic: geographic location (country and/or sea)

text: uncultivated viral genomes

text: human feces

text: human gut metagenome

text: https://doi.org/10.1126/science.aan4834; https://doi.org/10.1080/19490976.2018.1494103

text: Siphoviridae environmental samples

text: non-segmented

text: dsDNA

text: Human gut UViGs

text: NCBI GenBank downloaded using INPHARED v1.6 (27 Feb 2022)

characteristic: reference for biomaterial

characteristic: sample collection device or method

characteristic: sample derived from

text: Siphoviridae environmental samples

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: similarity search method

text: metagenome (not viral targeted)

characteristic: source of UViGs

characteristic: tRNA extraction software

text: Taxonomic predictions from VPF-Class (https://github.com/biocom-uib/vpf-tools, c8422bc, default params) and from DIAMOND (v2.0.9, --sensitive -k 10 --query-cover 50 --subject-cover 50 --evalue 1e-5) vs NCBI GenBank database compiled using INPHARED v1.6 (run 27 Feb 2022) were aggregated and the lowest agreeing taxonomy was used.

characteristic: taxonomic classification

characteristic: taxonomy of predicted host

characteristic: title

text: 95% ANI;85% AF;greedy, centroid-based

characteristic: vOTU classification approach

text: MGV (https://doi.org/10.1038/s41564-021-00928-6);1.0

characteristic: vOTU database

characteristic: vOTU representative

text: blastn;2.10.1+;-max_target_seqs 25000 -perc_identity 90

text: CheckV;0.8.1;end-to-end,>=medium-quality; MGV_viral_detection(https://github.com/snayfach/MGV);95c2d4d;defaults

text: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae


characteristic: BACPHLIP temperate score

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

text: Reads (ERR7803603) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (NA) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15.

characteristic: MAG coverage software

characteristic: Number of BLAST hits supporting host taxonomy

characteristic: Number of CRISPR spacer hits supporting host taxonomy

characteristic: SRA accession

text: Hadza_MoBio_hadza-A-D_C_6_1128__k127_1884726__flag_0__multi_450.9037__len_46937

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: CheckV; DTR (high-confidence)

characteristic: completeness approach

characteristic: completeness score

text: CheckV;0.8.1;end-to-end, database v1.0

characteristic: completeness software

text: This sample represents an uncultured Caudovirales virus assembled from ERR7803603.

characteristic: description

text: independent sequence (UViG)

characteristic: detection type

text: terrestrial biome

characteristic: environment (biome)

text: human-associated habitat

characteristic: environment (feature)

text: feces

text: Tanzania

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host prediction approach

text: uncultivated viral genomes

characteristic: investigation type

text: human feces

text: human gut metagenome

characteristic: metagenomic source

characteristic: nucleic acid extraction

text: uncultured Caudovirales phage

characteristic: organism

characteristic: percent of BLAST hits supporting viral taxonomy

text: non-segmented

characteristic: predicted genome structure

text: dsDNA

characteristic: predicted genome type

text: Human gut UViGs

characteristic: project name

characteristic: reference database(s)

text: uncultured Caudovirales phage

text: Illumina NovaSeq 6000

text: metagenome (not viral targeted)

characteristic: species-level group of predicted host

text: d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella;s__Prevotella copri

text: 95% ANI;85% AF;greedy, centroid-based

characteristic: vOTU sequence comparison approach

text: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales


characteristic: BACPHLIP temperate score

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

characteristic: MAG coverage software

characteristic: Number of BLAST hits supporting host taxonomy

characteristic: Number of CRISPR spacer hits supporting host taxonomy

characteristic: SRA accession

text: Hadza_MoBio_hadza-A-D_C_6_1128__k127_2138371__flag_0__multi_166.9257__len_22480

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics.

characteristic: assembly quality

characteristic: assembly software

text: CheckV; AAI-based (high-confidence)

characteristic: completeness approach

characteristic: completeness score

text: CheckV;0.8.1;end-to-end, database v1.0

characteristic: completeness software

characteristic: description

text: independent sequence (UViG)

characteristic: detection type

text: terrestrial biome

characteristic: environment (biome)

text: human-associated habitat

text: feces

characteristic: environment (material)

characteristic: feature prediction

text: Tanzania

characteristic: geographic location (country and/or sea)

text: CRISPR spacer matches (PILER-CR;v1.06 or CRT;v1.2; blastn;2.10.1+ -dust no -word_size 18, allowing <=1 gap or mismatch over 95% of spacer length) and >=1kb+>=96% identity BLAST hits (hs-blastn max_target_seqs 25000 -perc_identity 96 -evalue 1e-180) were used to identify connections between each viral contig and all non-viral contigs in the UHGG v1 (286,997 genomes) and this study (54,779 genomes). The predicted host for each viral contig is the lowest taxonomic rank with >70% agreement among all CRISPR+BLAST connections, as described previously (https://doi.org/10.1038/s41564-021-00928-6).

text: uncultivated viral genomes

text: human feces

text: human gut metagenome

text: Siphoviridae environmental samples

text: non-segmented

text: dsDNA

text: Human gut UViGs

characteristic: reference for biomaterial

characteristic: sample collection device or method

characteristic: sample derived from

text: Siphoviridae environmental samples

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: diamond;2.0.9;--sensitive -k 10 --query-cover 50 --subject-cover 50 --evalue 1e-5

characteristic: similarity search method

text: metagenome (not viral targeted)

characteristic: source of UViGs

characteristic: tRNA extraction software

characteristic: taxonomic classification

text: d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Oscillospiraceae;g__CAG-170;unk;unk

characteristic: taxonomy of predicted host

characteristic: title

text: 95% ANI;85% AF;greedy, centroid-based

characteristic: vOTU classification approach

characteristic: vOTU database

characteristic: vOTU representative

characteristic: viral identification software


characteristic: BACPHLIP temperate score

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

characteristic: MAG coverage software

characteristic: Number of BLAST hits supporting host taxonomy

characteristic: Number of CRISPR spacer hits supporting host taxonomy

characteristic: SRA accession

text: Hadza_MoBio_hadza-A-D_C_6_1128__k127_2581787__flag_0__multi_695.3699__len_68799

characteristic: Submitter Id

text: Multiple fragments where gaps span repetitive regions. Presence of the 23S, 16S and 5S rRNA genes and at least 18 tRNAs.

characteristic: assembly quality

characteristic: assembly software

text: CheckV; AAI-based (high-confidence)

characteristic: completeness approach

characteristic: completeness score

text: CheckV;0.8.1;end-to-end, database v1.0

characteristic: completeness software

text: This sample represents an uncultured Podoviridae virus assembled from ERR7803603.

characteristic: description

text: independent sequence (UViG)

characteristic: detection type

text: terrestrial biome

characteristic: environment (biome)

text: human-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: Tanzania

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host prediction approach

text: uncultivated viral genomes

characteristic: investigation type

text: human feces

text: human gut metagenome

characteristic: metagenomic source

text: https://doi.org/10.1126/science.aan4834; https://doi.org/10.1080/19490976.2018.1494103

characteristic: nucleic acid extraction

characteristic: number of standard tRNAs extracted

characteristic: number of unique standard tRNAs extracted

text: uncultured podovirus

characteristic: organism

characteristic: percent of BLAST hits supporting viral taxonomy

text: non-segmented

characteristic: predicted genome structure

text: dsDNA

characteristic: predicted genome type

text: Human gut UViGs

characteristic: project name

characteristic: reference database(s)

text: uncultured podovirus

text: Illumina NovaSeq 6000

text: metagenome (not viral targeted)

text: d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella;unk;unk

text: 95% ANI;85% AF;greedy, centroid-based

characteristic: vOTU sequence comparison approach

text: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae


characteristic: BACPHLIP temperate score

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

characteristic: MAG coverage software

characteristic: Number of BLAST hits supporting host taxonomy

characteristic: Number of CRISPR spacer hits supporting host taxonomy

characteristic: SRA accession

text: Hadza_MoBio_hadza-A-D_C_6_1128__k127_2692936__flag_0__multi_17.9960__len_84863

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: CheckV; AAI-based (medium-confidence)

characteristic: completeness approach

characteristic: completeness score

text: CheckV;0.8.1;end-to-end, database v1.0

characteristic: completeness software

text: This sample represents an uncultured Siphoviridae virus assembled from ERR7803603.

characteristic: description

text: independent sequence (UViG)

characteristic: detection type

text: terrestrial biome

characteristic: environment (biome)

text: human-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

characteristic: feature prediction

text: Tanzania

characteristic: geographic location (country and/or sea)

text: uncultivated viral genomes

text: human feces

text: human gut metagenome

text: Siphoviridae environmental samples

text: non-segmented

text: dsDNA

text: Human gut UViGs

text: NCBI GenBank downloaded using INPHARED v1.6 (27 Feb 2022)

characteristic: reference for biomaterial

characteristic: sample collection device or method

characteristic: sample derived from

text: Siphoviridae environmental samples

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: similarity search method

text: metagenome (not viral targeted)

characteristic: source of UViGs

characteristic: species-level group of predicted host

characteristic: tRNA extraction software

text: Taxonomic predictions from VPF-Class (https://github.com/biocom-uib/vpf-tools, c8422bc, default params) and from DIAMOND (v2.0.9, --sensitive -k 10 --query-cover 50 --subject-cover 50 --evalue 1e-5) vs NCBI GenBank database compiled using INPHARED v1.6 (run 27 Feb 2022) were aggregated and the lowest agreeing taxonomy was used.

characteristic: taxonomic classification

text: d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Lachnospirales;f__Lachnospiraceae;g__Eubacterium_F;s__Eubacterium_F sp000433735

characteristic: taxonomy of predicted host

characteristic: title

text: 95% ANI;85% AF;greedy, centroid-based

characteristic: vOTU classification approach

text: MGV (https://doi.org/10.1038/s41564-021-00928-6);1.0

characteristic: vOTU database

characteristic: vOTU representative

text: blastn;2.10.1+;-max_target_seqs 25000 -perc_identity 90

text: CheckV;0.8.1;end-to-end,>=medium-quality; MGV_viral_detection(https://github.com/snayfach/MGV);95c2d4d;defaults

text: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae


characteristic: BACPHLIP temperate score

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

text: Reads (ERR7803603) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (NA) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15.

characteristic: MAG coverage software

characteristic: Number of BLAST hits supporting host taxonomy

characteristic: Number of CRISPR spacer hits supporting host taxonomy

characteristic: SRA accession

text: Hadza_MoBio_hadza-A-D_C_6_1128__k127_2950841__flag_0__multi_32.9795__len_31360

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics.

characteristic: assembly quality

characteristic: assembly software

text: CheckV; AAI-based (high-confidence)

characteristic: completeness approach

characteristic: completeness score

text: CheckV;0.8.1;end-to-end, database v1.0

characteristic: completeness software

characteristic: description

text: independent sequence (UViG)

characteristic: detection type

text: terrestrial biome

characteristic: environment (biome)

text: human-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: Tanzania

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: CRISPR spacer matches (PILER-CR;v1.06 or CRT;v1.2; blastn;2.10.1+ -dust no -word_size 18, allowing <=1 gap or mismatch over 95% of spacer length) and >=1kb+>=96% identity BLAST hits (hs-blastn max_target_seqs 25000 -perc_identity 96 -evalue 1e-180) were used to identify connections between each viral contig and all non-viral contigs in the UHGG v1 (286,997 genomes) and this study (54,779 genomes). The predicted host for each viral contig is the lowest taxonomic rank with >70% agreement among all CRISPR+BLAST connections, as described previously (https://doi.org/10.1038/s41564-021-00928-6).

characteristic: host prediction approach

text: uncultivated viral genomes

characteristic: investigation type

text: human feces

text: human gut metagenome

characteristic: metagenomic source

characteristic: nucleic acid extraction

text: Siphoviridae environmental samples

characteristic: organism

characteristic: percent of BLAST hits supporting viral taxonomy

text: non-segmented

characteristic: predicted genome structure

text: dsDNA

characteristic: predicted genome type

text: Human gut UViGs

characteristic: project name

characteristic: reference database(s)

text: Siphoviridae environmental samples

text: Illumina NovaSeq 6000

text: diamond;2.0.9;--sensitive -k 10 --query-cover 50 --subject-cover 50 --evalue 1e-5

text: metagenome (not viral targeted)

text: d__Bacteria;p__Firmicutes_A;c__Clostridia_A;o__Christensenellales;f__CAG-74;g__SFMI01;s__SFMI01 sp004556155

text: 95% ANI;85% AF;greedy, centroid-based

characteristic: vOTU sequence comparison approach


characteristic: BACPHLIP temperate score

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

characteristic: MAG coverage software

characteristic: Number of BLAST hits supporting host taxonomy

characteristic: Number of CRISPR spacer hits supporting host taxonomy

characteristic: SRA accession

text: Hadza_MoBio_hadza-A-D_C_6_1128__k127_3073356__flag_0__multi_12.9712__len_51696

characteristic: Submitter Id

text: Multiple fragments where gaps span repetitive regions. Presence of the 23S, 16S and 5S rRNA genes and at least 18 tRNAs.

characteristic: assembly quality

characteristic: assembly software

text: CheckV; AAI-based (high-confidence)

characteristic: completeness approach

characteristic: completeness score

text: CheckV;0.8.1;end-to-end, database v1.0

characteristic: completeness software

text: This sample represents an uncultured Faecalibacterium virus Epona virus assembled from ERR7803603.

characteristic: description

text: independent sequence (UViG)

characteristic: detection type

text: terrestrial biome

characteristic: environment (biome)

text: human-associated habitat

text: feces

characteristic: feature prediction

text: Tanzania

characteristic: geographic location (country and/or sea)

text: uncultivated viral genomes

text: human feces

text: human gut metagenome

text: https://doi.org/10.1126/science.aan4834; https://doi.org/10.1080/19490976.2018.1494103

text: Myoviridae environmental samples

text: non-segmented

text: dsDNA

text: Human gut UViGs

characteristic: reference for biomaterial

characteristic: sample collection device or method

characteristic: sample derived from

text: Myoviridae environmental samples

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: similarity search method

text: metagenome (not viral targeted)

characteristic: source of UViGs

characteristic: tRNA extraction software

characteristic: taxonomic classification

text: d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Ruminococcaceae;g__Faecalibacterium;unk;unk

characteristic: taxonomy of predicted host

characteristic: title

text: 95% ANI;85% AF;greedy, centroid-based

characteristic: vOTU classification approach

characteristic: vOTU database

characteristic: vOTU representative

characteristic: viral identification software

text: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Eponavirus; Faecalibacterium virus Epona


characteristic: BACPHLIP temperate score

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

characteristic: MAG coverage software

characteristic: Number of BLAST hits supporting host taxonomy

characteristic: Number of CRISPR spacer hits supporting host taxonomy

characteristic: SRA accession

text: Hadza_MoBio_hadza-A-D_C_6_1128__k127_3227887__flag_0__multi_10.9945__len_36780

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: CheckV; AAI-based (high-confidence)

characteristic: completeness approach

characteristic: completeness score

text: CheckV;0.8.1;end-to-end, database v1.0

characteristic: completeness software

text: This sample represents an uncultured Siphoviridae virus assembled from ERR7803603.

characteristic: description

text: independent sequence (UViG)

characteristic: detection type

text: terrestrial biome

characteristic: environment (biome)

text: human-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: Tanzania

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host prediction approach

text: uncultivated viral genomes

characteristic: investigation type

text: human feces

text: human gut metagenome

characteristic: metagenomic source

characteristic: nucleic acid extraction

text: Siphoviridae environmental samples

characteristic: organism

characteristic: percent of BLAST hits supporting viral taxonomy

text: non-segmented

characteristic: predicted genome structure

text: dsDNA

characteristic: predicted genome type

text: Human gut UViGs

characteristic: project name

text: NCBI GenBank downloaded using INPHARED v1.6 (27 Feb 2022)

characteristic: reference database(s)

text: Siphoviridae environmental samples

text: Illumina NovaSeq 6000

text: metagenome (not viral targeted)

text: Taxonomic predictions from VPF-Class (https://github.com/biocom-uib/vpf-tools, c8422bc, default params) and from DIAMOND (v2.0.9, --sensitive -k 10 --query-cover 50 --subject-cover 50 --evalue 1e-5) vs NCBI GenBank database compiled using INPHARED v1.6 (run 27 Feb 2022) were aggregated and the lowest agreeing taxonomy was used.

text: d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Ruminococcaceae;g__Gemmiger;unk;unk

text: 95% ANI;85% AF;greedy, centroid-based

characteristic: vOTU sequence comparison approach


characteristic: BACPHLIP temperate score

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

text: Reads (ERR7803603) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (NA) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15.

characteristic: MAG coverage software

characteristic: Number of BLAST hits supporting host taxonomy

characteristic: Number of CRISPR spacer hits supporting host taxonomy

characteristic: SRA accession

text: Hadza_MoBio_hadza-A-D_C_6_1128__k127_3379072__flag_0__multi_15.5785__len_35694

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: CheckV; AAI-based (high-confidence)

characteristic: completeness approach

characteristic: completeness score

text: CheckV;0.8.1;end-to-end, database v1.0

characteristic: completeness software

text: This sample represents an uncultured Myoviridae virus assembled from ERR7803603.

characteristic: description

text: independent sequence (UViG)

characteristic: detection type

text: terrestrial biome

characteristic: environment (biome)

text: human-associated habitat

text: feces

characteristic: feature prediction

text: Tanzania

characteristic: geographic location (country and/or sea)

text: uncultivated viral genomes

text: human feces

text: human gut metagenome

text: Myoviridae environmental samples

text: non-segmented

text: dsDNA

text: Human gut UViGs

characteristic: reference for biomaterial

characteristic: sample collection device or method

characteristic: sample derived from

text: Myoviridae environmental samples

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: similarity search method

text: metagenome (not viral targeted)

characteristic: source of UViGs

characteristic: species-level group of predicted host

characteristic: tRNA extraction software

characteristic: taxonomic classification

text: d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Lachnospirales;f__Lachnospiraceae;g__Clostridium_Q;s__Clostridium_Q sp003024715

characteristic: taxonomy of predicted host

characteristic: title

text: 95% ANI;85% AF;greedy, centroid-based

characteristic: vOTU classification approach

text: MGV (https://doi.org/10.1038/s41564-021-00928-6);1.0

characteristic: vOTU database

characteristic: vOTU representative

text: blastn;2.10.1+;-max_target_seqs 25000 -perc_identity 90

text: CheckV;0.8.1;end-to-end,>=medium-quality; MGV_viral_detection(https://github.com/snayfach/MGV);95c2d4d;defaults

text: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae


characteristic: BACPHLIP temperate score

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

characteristic: MAG coverage software

characteristic: Number of BLAST hits supporting host taxonomy

characteristic: Number of CRISPR spacer hits supporting host taxonomy

characteristic: SRA accession

text: Hadza_MoBio_hadza-A-D_C_6_1128__k127_3534773__flag_0__multi_26.0000__len_45046

characteristic: Submitter Id

text: Multiple fragments where gaps span repetitive regions. Presence of the 23S, 16S and 5S rRNA genes and at least 18 tRNAs.

characteristic: assembly quality

characteristic: assembly software

text: CheckV; AAI-based (high-confidence)

characteristic: completeness approach

characteristic: completeness score

text: CheckV;0.8.1;end-to-end, database v1.0

characteristic: completeness software

characteristic: description

text: independent sequence (UViG)

characteristic: detection type

text: terrestrial biome

characteristic: environment (biome)

text: human-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: Tanzania

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: CRISPR spacer matches (PILER-CR;v1.06 or CRT;v1.2; blastn;2.10.1+ -dust no -word_size 18, allowing <=1 gap or mismatch over 95% of spacer length) and >=1kb+>=96% identity BLAST hits (hs-blastn max_target_seqs 25000 -perc_identity 96 -evalue 1e-180) were used to identify connections between each viral contig and all non-viral contigs in the UHGG v1 (286,997 genomes) and this study (54,779 genomes). The predicted host for each viral contig is the lowest taxonomic rank with >70% agreement among all CRISPR+BLAST connections, as described previously (https://doi.org/10.1038/s41564-021-00928-6).

characteristic: host prediction approach

text: uncultivated viral genomes

characteristic: investigation type

text: human feces

text: human gut metagenome

characteristic: metagenomic source

characteristic: nucleic acid extraction

text: Siphoviridae environmental samples

characteristic: organism

characteristic: percent of BLAST hits supporting viral taxonomy

text: non-segmented

characteristic: predicted genome structure

text: dsDNA

characteristic: predicted genome type

text: Human gut UViGs

characteristic: project name

characteristic: reference database(s)

text: Siphoviridae environmental samples

text: Illumina NovaSeq 6000

text: diamond;2.0.9;--sensitive -k 10 --query-cover 50 --subject-cover 50 --evalue 1e-5

text: metagenome (not viral targeted)

text: d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Oscillospiraceae;g__CAG-110;unk;unk

text: 95% ANI;85% AF;greedy, centroid-based

characteristic: vOTU sequence comparison approach

characteristic: viral identification software

text: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae


characteristic: BACPHLIP temperate score

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

characteristic: MAG coverage software

characteristic: Number of BLAST hits supporting host taxonomy

characteristic: Number of CRISPR spacer hits supporting host taxonomy

characteristic: SRA accession

text: Hadza_MoBio_hadza-A-D_C_6_1128__k127_3571511__flag_3__multi_245.0058__len_42568

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: CheckV; DTR (high-confidence)

characteristic: completeness approach

characteristic: completeness score

text: CheckV;0.8.1;end-to-end, database v1.0

characteristic: completeness software

text: This sample represents an uncultured Siphoviridae virus assembled from ERR7803603.

characteristic: description

text: independent sequence (UViG)

characteristic: detection type

text: terrestrial biome

characteristic: environment (biome)

text: human-associated habitat

characteristic: environment (feature)

text: feces

characteristic: feature prediction

text: Tanzania

characteristic: geographic location (country and/or sea)

text: uncultivated viral genomes

text: human feces

text: human gut metagenome

text: https://doi.org/10.1126/science.aan4834; https://doi.org/10.1080/19490976.2018.1494103

text: Siphoviridae environmental samples

text: non-segmented

text: dsDNA

text: Human gut UViGs

text: NCBI GenBank downloaded using INPHARED v1.6 (27 Feb 2022)

characteristic: reference for biomaterial

characteristic: sample collection device or method

characteristic: sample derived from

text: Siphoviridae environmental samples

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: similarity search method

text: metagenome (not viral targeted)

characteristic: source of UViGs

characteristic: species-level group of predicted host

characteristic: tRNA extraction software

characteristic: taxonomic classification

text: d__Bacteria;p__Firmicutes_A;c__Clostridia_A;o__Christensenellales;f__CAG-74;g__SFFS01;s__SFFS01 sp004557805

characteristic: taxonomy of predicted host

characteristic: title

text: 95% ANI;85% AF;greedy, centroid-based

characteristic: vOTU classification approach

characteristic: vOTU database

characteristic: vOTU representative


characteristic: BACPHLIP temperate score

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

text: Reads (ERR7803603) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (NA) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15.

characteristic: MAG coverage software

characteristic: Number of BLAST hits supporting host taxonomy

characteristic: Number of CRISPR spacer hits supporting host taxonomy

characteristic: SRA accession

text: Hadza_MoBio_hadza-A-D_C_6_1128__k127_446444__flag_0__multi_14.9976__len_65035

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics.

characteristic: assembly quality

characteristic: assembly software

text: CheckV; AAI-based (high-confidence)

characteristic: completeness approach

characteristic: completeness score

text: CheckV;0.8.1;end-to-end, database v1.0

characteristic: completeness software

text: This sample represents an uncultured Andromedavirus virus assembled from ERR7803603.

characteristic: description

text: independent sequence (UViG)

characteristic: detection type

text: terrestrial biome

characteristic: environment (biome)

text: human-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: Tanzania

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host prediction approach

text: uncultivated viral genomes

characteristic: investigation type

text: human feces

text: human gut metagenome

characteristic: metagenomic source

characteristic: nucleic acid extraction

characteristic: number of standard tRNAs extracted

characteristic: number of unique standard tRNAs extracted

text: Siphoviridae environmental samples

characteristic: organism

characteristic: percent of BLAST hits supporting viral taxonomy

text: non-segmented

characteristic: predicted genome structure

text: dsDNA

characteristic: predicted genome type

text: Human gut UViGs

characteristic: project name

characteristic: reference database(s)

text: Siphoviridae environmental samples

text: Illumina NovaSeq 6000

text: metagenome (not viral targeted)

text: Taxonomic predictions from VPF-Class (https://github.com/biocom-uib/vpf-tools, c8422bc, default params) and from DIAMOND (v2.0.9, --sensitive -k 10 --query-cover 50 --subject-cover 50 --evalue 1e-5) vs NCBI GenBank database compiled using INPHARED v1.6 (run 27 Feb 2022) were aggregated and the lowest agreeing taxonomy was used.

text: d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella;unk;unk

text: 95% ANI;85% AF;greedy, centroid-based

characteristic: vOTU sequence comparison approach

text: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Andromedavirus


characteristic: BACPHLIP temperate score

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

characteristic: MAG coverage software

characteristic: Number of BLAST hits supporting host taxonomy

characteristic: Number of CRISPR spacer hits supporting host taxonomy

characteristic: SRA accession

text: Hadza_MoBio_hadza-A-D_C_6_1128__k127_622341__flag_0__multi_13.9940__len_21873

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: CheckV; AAI-based (high-confidence)

characteristic: completeness approach

characteristic: completeness score

text: CheckV;0.8.1;end-to-end, database v1.0

characteristic: completeness software

text: This sample represents an uncultured Caudovirales virus assembled from ERR7803603.

characteristic: description

text: independent sequence (UViG)

characteristic: detection type

text: terrestrial biome

characteristic: environment (biome)

text: human-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

characteristic: feature prediction

text: Tanzania

characteristic: geographic location (country and/or sea)

text: uncultivated viral genomes

text: human feces

text: human gut metagenome

text: uncultured Caudovirales phage

text: non-segmented

text: dsDNA

text: Human gut UViGs

characteristic: reference for biomaterial

characteristic: sample collection device or method

characteristic: sample derived from

text: uncultured Caudovirales phage

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: similarity search method

text: metagenome (not viral targeted)

characteristic: source of UViGs

characteristic: tRNA extraction software

characteristic: taxonomic classification

text: d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Oscillospiraceae;unk;unk;unk

characteristic: taxonomy of predicted host

characteristic: title

text: 95% ANI;85% AF;greedy, centroid-based

characteristic: vOTU classification approach

text: MGV (https://doi.org/10.1038/s41564-021-00928-6);1.0

characteristic: vOTU database

characteristic: vOTU representative

text: blastn;2.10.1+;-max_target_seqs 25000 -perc_identity 90

text: CheckV;0.8.1;end-to-end,>=medium-quality; MGV_viral_detection(https://github.com/snayfach/MGV);95c2d4d;defaults

characteristic: viral identification software

text: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales


characteristic: BACPHLIP temperate score

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

characteristic: MAG coverage software

characteristic: Number of BLAST hits supporting host taxonomy

characteristic: Number of CRISPR spacer hits supporting host taxonomy

characteristic: SRA accession

text: Hadza_MoBio_hadza-A-D_C_6_1128__k127_833399__flag_0__multi_39.8909__len_22922

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: CheckV; AAI-based (high-confidence)

characteristic: completeness approach

characteristic: completeness score

text: CheckV;0.8.1;end-to-end, database v1.0

characteristic: completeness software

text: This sample represents an uncultured Roseburia phage Jekyll virus assembled from ERR7803603.

characteristic: description

text: independent sequence (UViG)

characteristic: detection type

text: terrestrial biome

characteristic: environment (biome)

text: human-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: Tanzania

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: CRISPR spacer matches (PILER-CR;v1.06 or CRT;v1.2; blastn;2.10.1+ -dust no -word_size 18, allowing <=1 gap or mismatch over 95% of spacer length) and >=1kb+>=96% identity BLAST hits (hs-blastn max_target_seqs 25000 -perc_identity 96 -evalue 1e-180) were used to identify connections between each viral contig and all non-viral contigs in the UHGG v1 (286,997 genomes) and this study (54,779 genomes). The predicted host for each viral contig is the lowest taxonomic rank with >70% agreement among all CRISPR+BLAST connections, as described previously (https://doi.org/10.1038/s41564-021-00928-6).

characteristic: host prediction approach

text: uncultivated viral genomes

characteristic: investigation type

text: human feces

text: human gut metagenome

characteristic: metagenomic source

text: https://doi.org/10.1126/science.aan4834; https://doi.org/10.1080/19490976.2018.1494103

characteristic: nucleic acid extraction

text: uncultured virus

characteristic: organism

characteristic: percent of BLAST hits supporting viral taxonomy

text: non-segmented

characteristic: predicted genome structure

text: uncharacterized

characteristic: predicted genome type

text: Human gut UViGs

characteristic: project name

characteristic: reference database(s)

text: uncultured virus

text: Illumina NovaSeq 6000

text: diamond;2.0.9;--sensitive -k 10 --query-cover 50 --subject-cover 50 --evalue 1e-5

text: metagenome (not viral targeted)

text: d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Lachnospirales;f__Lachnospiraceae;unk;unk;unk

text: 95% ANI;85% AF;greedy, centroid-based

characteristic: vOTU sequence comparison approach

text: Viruses; unclassified bacterial viruses; Roseburia phage Jekyll


characteristic: BACPHLIP temperate score

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

text: Reads (ERR7803603) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (NA) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15.

characteristic: MAG coverage software

characteristic: Number of BLAST hits supporting host taxonomy

characteristic: Number of CRISPR spacer hits supporting host taxonomy

characteristic: SRA accession

text: Hadza_MoBio_hadza-A-D_C_6_1128__k127_992917__flag_1__multi_11.0000__len_39869

characteristic: Submitter Id

text: Multiple fragments where gaps span repetitive regions. Presence of the 23S, 16S and 5S rRNA genes and at least 18 tRNAs.

characteristic: assembly quality

characteristic: assembly software

text: CheckV; AAI-based (medium-confidence)

characteristic: completeness approach

characteristic: completeness score

text: CheckV;0.8.1;end-to-end, database v1.0

characteristic: completeness software

text: This sample represents an uncultured Siphoviridae virus assembled from ERR7803603.

characteristic: description

text: independent sequence (UViG)

characteristic: detection type

text: terrestrial biome

characteristic: environment (biome)

text: human-associated habitat

text: feces

characteristic: feature prediction

text: Tanzania

characteristic: geographic location (country and/or sea)

text: uncultivated viral genomes

text: human feces

text: human gut metagenome

text: Siphoviridae environmental samples

text: non-segmented

text: dsDNA

text: Human gut UViGs

text: NCBI GenBank downloaded using INPHARED v1.6 (27 Feb 2022)

characteristic: reference for biomaterial

characteristic: sample collection device or method

characteristic: sample derived from

text: Siphoviridae environmental samples

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: similarity search method

text: metagenome (not viral targeted)

characteristic: source of UViGs

characteristic: species-level group of predicted host

characteristic: tRNA extraction software

text: Taxonomic predictions from VPF-Class (https://github.com/biocom-uib/vpf-tools, c8422bc, default params) and from DIAMOND (v2.0.9, --sensitive -k 10 --query-cover 50 --subject-cover 50 --evalue 1e-5) vs NCBI GenBank database compiled using INPHARED v1.6 (run 27 Feb 2022) were aggregated and the lowest agreeing taxonomy was used.

characteristic: taxonomic classification

text: d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Lachnospirales;f__Lachnospiraceae;g__UBA7160;s__UBA7160 sp902363135

characteristic: taxonomy of predicted host

characteristic: title

text: 95% ANI;85% AF;greedy, centroid-based

characteristic: vOTU classification approach

characteristic: vOTU database

characteristic: vOTU representative

text: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae


Ssi mRNA

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF OSLO

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Ssi mRNA

tag: Namespace:UNIVERSITY OF OSLO

characteristic: Submitter Id

text: mixed

text: mRNAs from Sphaeroforma sirkka

characteristic: description

text: mixed

text: Sphaeroforma sirkka

characteristic: organism

text: Sphaeroforma sirkka

text: Ssi mRNA


E-MTAB-11549:Sample 1

text: NEWCASTLE UNIVERSITY

text: public

text: E-MTAB-11549:Sample 1

tag: Namespace:NEWCASTLE UNIVERSITY

unit: week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

text: Protocols: Samples were collected by the Human Developmental Biology Resource from the clinic and transported to the lab immediately before dissection. Yolk sacs were digested for 30 mins using collagenase type 4 to create a single cell suspension. Cells were FACS sorted based on their protein expression of CD34. Contiguously placed CD34 positive and CD34 negative gates were used so to sample the full spectrum of expression. Cells were sorted into FACS tubes containing flow buffer coated with FCS then subsequently used for CITE-seq. Nucleic acids were extracted following the manufacturers guidelines for the 10x Genomics Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry) (CG000204_ChromiumNextGEMSingleCell3'v3.1_Rev D.pdf). CITE-Seq libraries were amplified following the TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3' Reagent Kit v3 or v3.1 (Single Index) Protocol (https://www.biolegend.com/en-us/protocols/totalseq-a-antibodies-and-cell-hashing-with-10x-single-cell-3-reagent-kit-v3-3-1-protocol). Nucleic acid libraries were constructed following the manufacturers guidelines for the 10x Genomics Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry) (CG000204_ChromiumNextGEMSingleCell3'v3.1_Rev D.pdf). CITE-Seq libraries were constructed following the TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3' Reagent Kit v3 or v3.1 (Single Index) Protocol (https://www.biolegend.com/en-us/protocols/totalseq-a-antibodies-and-cell-hashing-with-10x-single-cell-3-reagent-kit-v3-3-1-protocol).

text: embryo stage

characteristic: developmental stage

text: normal

characteristic: disease

text: Carnegie stage 16 to 19

characteristic: embryo stage

characteristic: immunophenotype

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: yolk sac

characteristic: organism part

text: Homo sapiens

text: female

characteristic: sex

text: Sample 1


E-MTAB-11549:Sample 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NEWCASTLE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11549:Sample 2

text: ArrayExpress

text: human

text: embryo stage

text: normal

text: Carnegie stage 16 to 19

text: Homo sapiens

text: Homo sapiens

text: yolk sac

text: Homo sapiens

text: female

text: Sample 2

characteristic: title


text: The Czech Academy of Sciences (CAS)

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:The Czech Academy of Sciences (CAS)

characteristic: Submitter Id

text: Greenhouse, Flooding, Heat, CO2

characteristic: climate environment

characteristic: collection date

text: wild strawberry

text: Sample collected from a mature leaf. Samples with the same sample alias but different M value (e.g. FV_FR_01_01_P0_HPT_M1 and FV_FR_01_01_P0_HPT_M2), represent biological replicates (samples collected from different plants exposed to the same conditions).

characteristic: description

text: France

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Pubmed ID or DOI

characteristic: isolation and growth condition

text: Fragaria vesca

characteristic: organism

characteristic: plant developmental stage

characteristic: plant growth medium

characteristic: plant structure

characteristic: ploidy

text: Fragaria vesca


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Czech Academy of Sciences (CAS)

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Greenhouse, Heat, CO2

text: wild strawberry

characteristic: common name

text: France

text: Pubmed ID or DOI

text: Fragaria vesca

text: Fragaria vesca

characteristic: title


text: The Czech Academy of Sciences (CAS)

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Greenhouse, Drought, Heat, CO2

characteristic: climate environment

characteristic: collection date

text: wild strawberry

characteristic: description

text: France

characteristic: geographic location (country and/or sea)

text: Pubmed ID or DOI

text: Fragaria vesca

text: Fragaria vesca


text: The Czech Academy of Sciences (CAS)

text: public

text: Greenhouse, Flooding, Heat

text: wild strawberry

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Pubmed ID or DOI

characteristic: isolation and growth condition

text: Fragaria vesca

characteristic: organism

characteristic: plant developmental stage

characteristic: plant growth medium

characteristic: plant structure

characteristic: ploidy

text: Fragaria vesca


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Czech Academy of Sciences (CAS)

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Greenhouse, Heat

text: wild strawberry

characteristic: common name

text: France

text: Pubmed ID or DOI

text: Fragaria vesca

text: Fragaria vesca

characteristic: title


text: The Czech Academy of Sciences (CAS)

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Greenhouse, Drought, Heat

characteristic: climate environment

characteristic: collection date

text: wild strawberry

characteristic: description

text: France

characteristic: geographic location (country and/or sea)

text: Pubmed ID or DOI

text: Fragaria vesca

text: Fragaria vesca


text: The Czech Academy of Sciences (CAS)

text: public

text: Common Garden, Flooding

text: wild strawberry

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Pubmed ID or DOI

characteristic: isolation and growth condition

text: Fragaria vesca

characteristic: organism

characteristic: plant developmental stage

characteristic: plant growth medium

characteristic: plant structure

characteristic: ploidy

text: Fragaria vesca


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Czech Academy of Sciences (CAS)

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Common Garden, Control

text: wild strawberry

characteristic: common name

text: France

text: Pubmed ID or DOI

text: Fragaria vesca

text: Fragaria vesca

characteristic: title


text: The Czech Academy of Sciences (CAS)

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Common Garden, Drougth

characteristic: climate environment

characteristic: collection date

text: wild strawberry

characteristic: description

text: France

characteristic: geographic location (country and/or sea)

text: Pubmed ID or DOI

text: Fragaria vesca

text: Fragaria vesca


text: INRAE Jouy-en-Josas

text: public

tag: Namespace:INRAE Jouy-en-Josas

text: rainbow trout

text: INRAE isogenic line 01 individual 1

text: Oncorhynchus mykiss

characteristic: organism

text: Oncorhynchus mykiss

text: female

characteristic: sex

text: fin


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: INRAE Jouy-en-Josas

text: public

text: rainbow trout

text: INRAE isogenic line 01 individual 2

text: Oncorhynchus mykiss

text: Oncorhynchus mykiss

text: female

text: fin


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRAE Jouy-en-Josas

text: public

text: rainbow trout

text: INRAE isogenic line 02

text: Oncorhynchus mykiss

text: Oncorhynchus mykiss

text: female

text: fin


text: INRAE Jouy-en-Josas

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: rainbow trout

text: INRAE isogenic line 03

text: Oncorhynchus mykiss

text: Oncorhynchus mykiss

text: female

text: fin


text: INRAE Jouy-en-Josas

text: public

text: rainbow trout

characteristic: common name

text: INRAE isogenic line 04

text: Oncorhynchus mykiss

text: Oncorhynchus mykiss

text: female

text: fin

characteristic: title


text: INRAE Jouy-en-Josas

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: rainbow trout

text: INRAE isogenic line 05

text: Oncorhynchus mykiss

text: Oncorhynchus mykiss

text: female

text: fin


text: INRAE Jouy-en-Josas

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: rainbow trout

text: INRAE isogenic line 06

text: Oncorhynchus mykiss

text: Oncorhynchus mykiss

text: female

text: fin


text: INRAE Jouy-en-Josas

text: public

text: rainbow trout

text: INRAE isogenic line 07

text: Oncorhynchus mykiss

text: Oncorhynchus mykiss

text: female

text: fin


text: INRAE Jouy-en-Josas

text: public

text: rainbow trout

text: INRAE isogenic line 08

text: Oncorhynchus mykiss

text: Oncorhynchus mykiss

text: female

text: fin


text: INRAE Jouy-en-Josas

text: public

text: rainbow trout

text: INRAE isogenic line 09

text: Oncorhynchus mykiss

text: Oncorhynchus mykiss

text: female

text: fin


text: INRAE Jouy-en-Josas

text: public

text: rainbow trout

text: INRAE isogenic line 10

text: Oncorhynchus mykiss

text: Oncorhynchus mykiss

text: female

text: fin


text: INRAE Jouy-en-Josas

text: public

text: rainbow trout

text: INRAE isogenic line 11

text: Oncorhynchus mykiss

text: Oncorhynchus mykiss

text: female

text: fin


Sta mRNA

text: UNIVERSITY OF OSLO

text: public

text: Sta mRNA

text: mixed

text: mRNAs from Sphaeroforma tapetis

text: mixed

text: Sphaeroforma tapetis

text: Sphaeroforma tapetis

text: Sta mRNA


Sta gDNA

text: UNIVERSITY OF OSLO

text: public

text: Sta gDNA

text: mixed

text: genomic DNA from Sphaeroforma tapetis

text: mixed

text: Sphaeroforma tapetis

text: Sphaeroforma tapetis

text: Sta gDNA


text: KAUST

text: public

characteristic: collection date

text: bread wheat

text: introgression line Sr43 wild type

text: Israel

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Greenhouse

characteristic: isolation and growth condition

text: Triticum aestivum

text: seedlings

characteristic: plant developmental stage

text: soil mix

characteristic: plant growth medium

text: Roots

characteristic: plant structure

text: Triticum aestivum


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: KAUST

text: public

text: bread wheat

text: introgression line Sr43 susceptible EMS mutant

characteristic: description

text: Israel

characteristic: geographic location (country and/or sea)

text: Greenhouse

text: Triticum aestivum

characteristic: organism

text: seedlings

text: soil mix

text: Roots

text: Triticum aestivum


characteristic: External Id

text: Dalhousie University

characteristic: GAL

text: NOT PROVIDED

text: Tree of Life Programme

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

tag: Namespace:Tree of Life Programme

text: BRENT ROBICHEAU | JENNIFER TOLMAN | JULIE LAROCHE

characteristic: collected by

text: DALHOUSIE UNIVERSITY | DALHOUSIE UNIVERSITY | DALHOUSIE UNIVERSITY

characteristic: collecting institution

unit: m

characteristic: depth

text: Canada

text: Nova Scotia | Halifax | Dalhousie University | Department of Biology

characteristic: geographic location (region and locality)

text: sea water

characteristic: habitat

text: BRENT ROBICHEAU | JULIE LAROCHE

characteristic: identified by

text: not provided

characteristic: lifestage

text: Braarudosphaera bigelowii

text: WHOLE ORGANISM

characteristic: organism part

text: ASG

characteristic: project name

text: Braarudosphaera bigelowii

text: NOT COLLECTED

characteristic: sex

characteristic: specimen id

text: NOT PROVIDED

characteristic: specimen voucher

text: N

characteristic: symbiont

characteristic: title

characteristic: tolid


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Dalhousie University

characteristic: GAL

text: NOT PROVIDED

text: Tree of Life Programme

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: sea water

text: BRENT ROBICHEAU | JULIE LAROCHE

text: DALHOUSIE UNIVERSITY | DALHOUSIE UNIVERSITY

text: not provided

text: Braarudosphaera bigelowii

characteristic: organism

text: WHOLE ORGANISM

text: ASG

text: Braarudosphaera bigelowii

text: NOT COLLECTED

text: NOT PROVIDED

text: N


text: Dalhousie University

text: Tree of Life Programme

characteristic: INSDC last update

text: public

characteristic: collected by

characteristic: collecting institution

characteristic: depth

text: Canada

characteristic: geographic location (region and locality)

text: sea water

characteristic: habitat

text: BRENT ROBICHEAU | JULIE LAROCHE

characteristic: identified by

text: not provided

characteristic: lifestage

text: Braarudosphaera bigelowii

text: ASG

characteristic: project name

characteristic: sample derived from

text: Braarudosphaera bigelowii

text: NOT COLLECTED

characteristic: sex

characteristic: specimen id

text: NOT PROVIDED

characteristic: specimen voucher

text: N

characteristic: symbiont

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:GFZ German Research Centre for Geosciences

characteristic: Submitter Id

characteristic: collection date

text: library1 prokayotic 16S rRNA-ITS of core depth 1-2 cm

characteristic: description

text: aquatic

characteristic: environment (biome)

text: lake

characteristic: environment (feature)

text: sediment

characteristic: environment (material)

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: sediment metagenome

text: Anthropogenic-induced increase in lake cyanobacteria abundance since the Bronze Age revealed by sedimentary ancient DNA reveals

text: sediment metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina MiSeq v3 2x300bp

characteristic: sequencing method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: GFZ German Research Centre for Geosciences

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: library1 prokayotic 16S rRNA-ITS of core depth 2-3 cm

text: aquatic

text: lake

text: sediment

text: Germany

text: mimarks-survey

text: sediment metagenome

characteristic: organism

characteristic: project name

text: sediment metagenome

text: sediment

text: Illumina MiSeq v3 2x300bp

characteristic: title


text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: library1 prokayotic 16S rRNA-ITS of core depth 3-4 cm

characteristic: description

text: aquatic

characteristic: environment (biome)

text: lake

characteristic: environment (feature)

text: sediment

characteristic: environment (material)

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: mimarks-survey

text: sediment metagenome

text: sediment metagenome

text: sediment

text: Illumina MiSeq v3 2x300bp


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: library1 prokayotic 16S rRNA-ITS of core depth 4-5 cm

text: aquatic

text: lake

text: sediment

text: Germany

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: sediment metagenome

text: sediment metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina MiSeq v3 2x300bp

characteristic: sequencing method


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: library1 prokayotic 16S rRNA-ITS of core depth 5-6 cm

text: aquatic

text: lake

text: sediment

text: Germany

text: mimarks-survey

text: sediment metagenome

characteristic: organism

characteristic: project name

text: sediment metagenome

text: sediment

text: Illumina MiSeq v3 2x300bp

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: library1 prokayotic 16S rRNA-ITS of core depth 6-7 cm

characteristic: description

text: aquatic

characteristic: environment (biome)

text: lake

characteristic: environment (feature)

text: sediment

characteristic: environment (material)

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: mimarks-survey

text: sediment metagenome

text: sediment metagenome

text: sediment

text: Illumina MiSeq v3 2x300bp


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: library1 prokayotic 16S rRNA-ITS of core depth 7-8 cm

text: aquatic

text: lake

text: sediment

text: Germany

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: sediment metagenome

text: sediment metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina MiSeq v3 2x300bp

characteristic: sequencing method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: library1 prokayotic 16S rRNA-ITS of core depth 8-9 cm

text: aquatic

text: lake

text: sediment

text: Germany

text: mimarks-survey

text: sediment metagenome

characteristic: organism

characteristic: project name

text: sediment metagenome

text: sediment

text: Illumina MiSeq v3 2x300bp

characteristic: title


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: library1 prokayotic 16S rRNA-ITS of core depth 9-10 cm

characteristic: description

text: aquatic

characteristic: environment (biome)

text: lake

characteristic: environment (feature)

text: sediment

characteristic: environment (material)

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: mimarks-survey

text: sediment metagenome

text: sediment metagenome

text: sediment

text: Illumina MiSeq v3 2x300bp


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: library1 prokayotic 16S rRNA-ITS of core depth 10-11 cm

text: aquatic

text: lake

text: sediment

text: Germany

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: sediment metagenome

text: sediment metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina MiSeq v3 2x300bp

characteristic: sequencing method


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: library1 prokayotic 16S rRNA-ITS of core depth 11-12 cm

text: aquatic

text: lake

text: sediment

text: Germany

text: mimarks-survey

text: sediment metagenome

characteristic: organism

characteristic: project name

text: sediment metagenome

text: sediment

text: Illumina MiSeq v3 2x300bp

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: library1 prokayotic 16S rRNA-ITS of core depth 15-16 cm

characteristic: description

text: aquatic

characteristic: environment (biome)

text: lake

characteristic: environment (feature)

text: sediment

characteristic: environment (material)

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: mimarks-survey

text: sediment metagenome

text: sediment metagenome

text: sediment

text: Illumina MiSeq v3 2x300bp


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: library1 prokayotic 16S rRNA-ITS of core depth 20-21 cm

text: aquatic

text: lake

text: sediment

text: Germany

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: sediment metagenome

text: sediment metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina MiSeq v3 2x300bp

characteristic: sequencing method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: library1 prokayotic 16S rRNA-ITS of core depth 25-26 cm

text: aquatic

text: lake

text: sediment

text: Germany

text: mimarks-survey

text: sediment metagenome

characteristic: organism

characteristic: project name

text: sediment metagenome

text: sediment

text: Illumina MiSeq v3 2x300bp

characteristic: title


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: library1 prokayotic 16S rRNA-ITS of core depth 30-31 cm

characteristic: description

text: aquatic

characteristic: environment (biome)

text: lake

characteristic: environment (feature)

text: sediment

characteristic: environment (material)

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: mimarks-survey

text: sediment metagenome

text: sediment metagenome

text: sediment

text: Illumina MiSeq v3 2x300bp


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: library1 prokayotic 16S rRNA-ITS of core depth 35-36 cm

text: aquatic

text: lake

text: sediment

text: Germany

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: sediment metagenome

text: sediment metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina MiSeq v3 2x300bp

characteristic: sequencing method


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: library1 prokayotic 16S rRNA-ITS of core depth 40-41 cm

text: aquatic

text: lake

text: sediment

text: Germany

text: mimarks-survey

text: sediment metagenome

characteristic: organism

text: Anthropogenic-induced increase in lake cyanobacteria abundance since the Bronze Age revealed by sedimentary ancient DNA reveals

characteristic: project name

text: sediment metagenome

text: sediment

text: Illumina MiSeq v3 2x300bp

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: GFZ German Research Centre for Geosciences

text: public

text: library1 prokayotic 16S rRNA-ITS of core depth 45-46 cm

characteristic: description

text: aquatic

characteristic: environment (biome)

text: lake

characteristic: environment (feature)

text: sediment

characteristic: environment (material)

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: mimarks-survey

text: sediment metagenome

text: sediment metagenome

text: sediment

text: Illumina MiSeq v3 2x300bp


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: library1 prokayotic 16S rRNA-ITS of core depth 50-51 cm

text: aquatic

text: lake

text: sediment

text: Germany

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: sediment metagenome

text: sediment metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina MiSeq v3 2x300bp

characteristic: sequencing method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: library1 prokayotic 16S rRNA-ITS of core depth 55-56 cm

text: aquatic

text: lake

text: sediment

text: Germany

text: mimarks-survey

text: sediment metagenome

characteristic: organism

characteristic: project name

text: sediment metagenome

text: sediment

text: Illumina MiSeq v3 2x300bp

characteristic: title


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: library1 prokayotic 16S rRNA-ITS of core depth 60-61 cm

characteristic: description

text: aquatic

characteristic: environment (biome)

text: lake

characteristic: environment (feature)

text: sediment

characteristic: environment (material)

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: mimarks-survey

text: sediment metagenome

text: sediment metagenome

text: sediment

text: Illumina MiSeq v3 2x300bp


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: library1 prokayotic 16S rRNA-ITS of core depth 65-66 cm

text: aquatic

text: lake

text: sediment

text: Germany

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: sediment metagenome

text: sediment metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina MiSeq v3 2x300bp

characteristic: sequencing method


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: library1 prokayotic 16S rRNA-ITS of core depth 210-211 cm

text: aquatic

text: lake

text: sediment

text: Germany

text: mimarks-survey

text: sediment metagenome

characteristic: organism

characteristic: project name

text: sediment metagenome

text: sediment

text: Illumina MiSeq v3 2x300bp

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: library1 prokayotic 16S rRNA-ITS of core depth 215-216 cm

characteristic: description

text: aquatic

characteristic: environment (biome)

text: lake

characteristic: environment (feature)

text: sediment

characteristic: environment (material)

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: mimarks-survey

text: sediment metagenome

text: sediment metagenome

text: sediment

text: Illumina MiSeq v3 2x300bp


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: library1 prokayotic 16S rRNA-ITS of core depth 220-221 cm

text: aquatic

text: lake

text: sediment

text: Germany

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: sediment metagenome

text: sediment metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina MiSeq v3 2x300bp

characteristic: sequencing method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: library1 prokayotic 16S rRNA-ITS of core depth 240-241 cm

text: aquatic

text: lake

text: sediment

text: Germany

text: mimarks-survey

text: sediment metagenome

characteristic: organism

characteristic: project name

text: sediment metagenome

text: sediment

text: Illumina MiSeq v3 2x300bp

characteristic: title


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: library1 prokayotic 16S rRNA-ITS of core depth 260-261 cm

characteristic: description

text: aquatic

characteristic: environment (biome)

text: lake

characteristic: environment (feature)

text: sediment

characteristic: environment (material)

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: mimarks-survey

text: sediment metagenome

text: sediment metagenome

text: sediment

text: Illumina MiSeq v3 2x300bp


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: library1 prokayotic 16S rRNA-ITS of core depth 280-281 cm

text: aquatic

text: lake

text: sediment

text: Germany

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: sediment metagenome

text: sediment metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina MiSeq v3 2x300bp

characteristic: sequencing method


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

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