characteristic: External Id

text: The Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40709_Track-80087: 131823; L40709_Track-79470: 265227

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

tag: Namespace:The Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: Protocols: hESC-derived pancreatic cells at Stage 4 Day 1 of the pancreatic differentiation protocol (Rezania et al., 2014; Petersen et al., 2017) were dissociated into single cells with TrypLE and resuspended into a FACS buffer containing 0.5% BSA and 2 mM EDTA (Thermo Fisher) in 1X PBS. The cells were index-sorted according to their GFP expression into 384-well plates on a BD FACSAria and snap-frozen. Cells are processed with SMARTseq-2 protocol miniaturized and modified based on Picelli et al. 2013 (doi: 10.1038/nmeth.2639). In short, cells are sorted in 0.5 µl 0.2% Triton X-100 solution with RNase Inhibitor into a 384-well plate, frozen at -80°C. After thawing, the cell lysate is mixed with 0.5 ul of a primer mix (containing 5 mM dNTP (Invitrogen), 0.5 uM dT-primer*, 1 U RNase Inhibitor (NEB)). RNA is denatured (3 minutes 72 °C), followed by reverse transcription (42 °C 90 min + 70°C 15 min) after adding 1.5 ul RT buffer mix (1x superscript II buffer (Invitrogen), 1 M betaine, 5 mM DTT, 6 mM MgCl2, 1 uM TSO-primer*, 2.3 U RNase Inhibitor and 25 U Superscript II). The cDNA is amplified using 1x Kapa HiFi HotStart Readymix (Roche) and 0.1 uM UP-primer under following cycling conditions: 98°C for 3 min, 23 cycles [98°C 20 sec, 67°C 15 sec, 72°C 6 min] and final elongation at 72°C for 5 min. The amplified cDNA is purified using 1x volume of Sera-Mag SpeedBeads (GE Healthcare) and eluted in 12 ul nuclease free water. For library preparation 1.5 µl cDNA is desiccated and rehydrated in 1 ul Tagmentation mix (1x TTBL buffer, 0.1 ul Tagment DNA Enzyme; from TruePrep DNA Library Prep Kit V2 for Illumina; Vazyme) and tagmented (55°C, 5 min), followed by an index PCR with 1x concentrated KAPA Hifi HotStart Ready Mix and 0.7 µM dual indexing primers (72°C 3 min, 98°C 30 sec, 13 cycles [98°C 10 sec, 63°C 20 sec, 72°C 1 min], 72°C 5 min). After PCR, libraries are quantified with AccuBlue Broad range chemistry (Biotium), equimolarly pooled and purified twice with 0.9x volume Sera-Mag SpeedBeads.

characteristic: description

text: embryo

text: normal

text: L40692_Track-80171: 219433; L40692_Track-79367: 440521

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP low

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40691_Track-80044: 144116; L40691_Track-79545: 291631

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

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text: embryo

text: normal

text: L40598_Track-80151: 243527; L40598_Track-79333: 485232

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP low

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

text: normal

text: L40606_Track-79967: 200834; L40606_Track-79573: 402721

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

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text: ArrayExpress

text: pancreatic cell

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characteristic: gfp mean

text: L40621_Track-80135: 214847; L40621_Track-79492: 432143

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

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text: public

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text: ArrayExpress

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text: pancreatic cell

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text: L40585_Track-79971: 174993; L40585_Track-79418: 351771

text: Homo sapiens

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text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP low

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

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characteristic: INSDC first public

text: public

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text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40676_Track-80148: 248174; L40676_Track-79366: 488653

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

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text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40650_Track-80070: 297646; L40650_Track-79412: 586327

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

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text: embryo

text: normal

text: L40664_Track-80243: 41431; L40664_Track-79640: 96703

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP low

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

text: normal

text: L40712_Track-80105: 241866; L40712_Track-79503: 483123

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

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text: public

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text: pancreatic cell

text: human

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text: embryo

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text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40589_Track-80185: 149693; L40589_Track-79424: 299626

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40708_Track-80049: 174382; L40708_Track-79520: 336490

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP low

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40683_Track-80008: 153304; L40683_Track-79522: 309410

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40542_Track-79963: 341255; L40542_Track-79448: 674455

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: The Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

characteristic: description

text: embryo

text: normal

text: L40556_Track-80029: 351082; L40556_Track-79315: 705557

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP low

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: Protocols: hESC-derived pancreatic cells at Stage 4 Day 1 of the pancreatic differentiation protocol (Rezania et al., 2014; Petersen et al., 2017) were dissociated into single cells with TrypLE and resuspended into a FACS buffer containing 0.5% BSA and 2 mM EDTA (Thermo Fisher) in 1X PBS. The cells were index-sorted according to their GFP expression into 384-well plates on a BD FACSAria and snap-frozen. Cells are processed with SMARTseq-2 protocol miniaturized and modified based on Picelli et al. 2013 (doi: 10.1038/nmeth.2639). In short, cells are sorted in 0.5 µl 0.2% Triton X-100 solution with RNase Inhibitor into a 384-well plate, frozen at -80°C. After thawing, the cell lysate is mixed with 0.5 ul of a primer mix (containing 5 mM dNTP (Invitrogen), 0.5 uM dT-primer*, 1 U RNase Inhibitor (NEB)). RNA is denatured (3 minutes 72 °C), followed by reverse transcription (42 °C 90 min + 70°C 15 min) after adding 1.5 ul RT buffer mix (1x superscript II buffer (Invitrogen), 1 M betaine, 5 mM DTT, 6 mM MgCl2, 1 uM TSO-primer*, 2.3 U RNase Inhibitor and 25 U Superscript II). The cDNA is amplified using 1x Kapa HiFi HotStart Readymix (Roche) and 0.1 uM UP-primer under following cycling conditions: 98°C for 3 min, 23 cycles [98°C 20 sec, 67°C 15 sec, 72°C 6 min] and final elongation at 72°C for 5 min. The amplified cDNA is purified using 1x volume of Sera-Mag SpeedBeads (GE Healthcare) and eluted in 12 ul nuclease free water. For library preparation 1.5 µl cDNA is desiccated and rehydrated in 1 ul Tagmentation mix (1x TTBL buffer, 0.1 ul Tagment DNA Enzyme; from TruePrep DNA Library Prep Kit V2 for Illumina; Vazyme) and tagmented (55°C, 5 min), followed by an index PCR with 1x concentrated KAPA Hifi HotStart Ready Mix and 0.7 µM dual indexing primers (72°C 3 min, 98°C 30 sec, 13 cycles [98°C 10 sec, 63°C 20 sec, 72°C 1 min], 72°C 5 min). After PCR, libraries are quantified with AccuBlue Broad range chemistry (Biotium), equimolarly pooled and purified twice with 0.9x volume Sera-Mag SpeedBeads.

text: embryo

text: normal

text: L40607_Track-80174: 118692; L40607_Track-79332: 237521

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40657_Track-79984: 185889; L40657_Track-79354: 369442

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40575_Track-79957: 313457; L40575_Track-79386: 628595

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP low

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40719_Track-79916: 186345; L40719_Track-79364: 376888

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40560_Track-80117: 109109; L40560_Track-79493: 216743

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

characteristic: description

text: embryo

text: normal

text: L40579_Track-80192: 316483; L40579_Track-79453: 635822

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP low

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

text: normal

text: L40552_Track-80005: 327229; L40552_Track-79546: 659174

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40592_Track-80021: 219299; L40592_Track-79564: 436492

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40717_Track-80168: 187411; L40717_Track-79506: 376012

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP low

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40564_Track-80146: 187157; L40564_Track-79406: 377639

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40595_Track-80162: 186997; L40595_Track-79461: 378678

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

characteristic: description

text: embryo

text: normal

text: L40667_Track-79959: 179064; L40667_Track-79416: 359124

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP low

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

text: normal

text: L40532_Track-79962: 274670; L40532_Track-79509: 548327

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40570_Track-80018: 177673; L40570_Track-79322: 351951

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

text: The Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40544_Track-80133: 177432; L40544_Track-79446: 356257

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP low

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: Protocols: hESC-derived pancreatic cells at Stage 4 Day 1 of the pancreatic differentiation protocol (Rezania et al., 2014; Petersen et al., 2017) were dissociated into single cells with TrypLE and resuspended into a FACS buffer containing 0.5% BSA and 2 mM EDTA (Thermo Fisher) in 1X PBS. The cells were index-sorted according to their GFP expression into 384-well plates on a BD FACSAria and snap-frozen. Cells are processed with SMARTseq-2 protocol miniaturized and modified based on Picelli et al. 2013 (doi: 10.1038/nmeth.2639). In short, cells are sorted in 0.5 µl 0.2% Triton X-100 solution with RNase Inhibitor into a 384-well plate, frozen at -80°C. After thawing, the cell lysate is mixed with 0.5 ul of a primer mix (containing 5 mM dNTP (Invitrogen), 0.5 uM dT-primer*, 1 U RNase Inhibitor (NEB)). RNA is denatured (3 minutes 72 °C), followed by reverse transcription (42 °C 90 min + 70°C 15 min) after adding 1.5 ul RT buffer mix (1x superscript II buffer (Invitrogen), 1 M betaine, 5 mM DTT, 6 mM MgCl2, 1 uM TSO-primer*, 2.3 U RNase Inhibitor and 25 U Superscript II). The cDNA is amplified using 1x Kapa HiFi HotStart Readymix (Roche) and 0.1 uM UP-primer under following cycling conditions: 98°C for 3 min, 23 cycles [98°C 20 sec, 67°C 15 sec, 72°C 6 min] and final elongation at 72°C for 5 min. The amplified cDNA is purified using 1x volume of Sera-Mag SpeedBeads (GE Healthcare) and eluted in 12 ul nuclease free water. For library preparation 1.5 µl cDNA is desiccated and rehydrated in 1 ul Tagmentation mix (1x TTBL buffer, 0.1 ul Tagment DNA Enzyme; from TruePrep DNA Library Prep Kit V2 for Illumina; Vazyme) and tagmented (55°C, 5 min), followed by an index PCR with 1x concentrated KAPA Hifi HotStart Ready Mix and 0.7 µM dual indexing primers (72°C 3 min, 98°C 30 sec, 13 cycles [98°C 10 sec, 63°C 20 sec, 72°C 1 min], 72°C 5 min). After PCR, libraries are quantified with AccuBlue Broad range chemistry (Biotium), equimolarly pooled and purified twice with 0.9x volume Sera-Mag SpeedBeads.

text: embryo

text: normal

text: L40689_Track-80028: 145801; L40689_Track-79325: 293193

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40596_Track-79930: 265095; L40596_Track-79355: 530710

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

characteristic: description

text: embryo

text: normal

text: L40661_Track-80238: 69500; L40661_Track-79622: 168173

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP low

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

text: normal

text: L40632_Track-80268: 108258; L40632_Track-79609: 257766

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40640_Track-80015: 118609; L40640_Track-79563: 228615

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40604_Track-80267: 154777; L40604_Track-79641: 373205

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP low

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40603_Track-80224: 195519; L40603_Track-79612: 479019

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40629_Track-80189: 235396; L40629_Track-79485: 469326

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

characteristic: description

text: embryo

text: normal

text: L40659_Track-79923: 146076; L40659_Track-79411: 288698

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP low

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

text: normal

text: L40707_Track-80101: 127163; L40707_Track-79344: 254716

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40599_Track-79947: 257017; L40599_Track-79425: 521789

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40538_Track-80002: 96909; L40538_Track-79517: 194189

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP low

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40618_Track-80053: 174804; L40618_Track-79342: 348990

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40557_Track-80037: 284735; L40557_Track-79327: 574121

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: The Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

characteristic: description

text: embryo

text: normal

text: L40703_Track-80242: 34071; L40703_Track-79617: 82905

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP low

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: Protocols: hESC-derived pancreatic cells at Stage 4 Day 1 of the pancreatic differentiation protocol (Rezania et al., 2014; Petersen et al., 2017) were dissociated into single cells with TrypLE and resuspended into a FACS buffer containing 0.5% BSA and 2 mM EDTA (Thermo Fisher) in 1X PBS. The cells were index-sorted according to their GFP expression into 384-well plates on a BD FACSAria and snap-frozen. Cells are processed with SMARTseq-2 protocol miniaturized and modified based on Picelli et al. 2013 (doi: 10.1038/nmeth.2639). In short, cells are sorted in 0.5 µl 0.2% Triton X-100 solution with RNase Inhibitor into a 384-well plate, frozen at -80°C. After thawing, the cell lysate is mixed with 0.5 ul of a primer mix (containing 5 mM dNTP (Invitrogen), 0.5 uM dT-primer*, 1 U RNase Inhibitor (NEB)). RNA is denatured (3 minutes 72 °C), followed by reverse transcription (42 °C 90 min + 70°C 15 min) after adding 1.5 ul RT buffer mix (1x superscript II buffer (Invitrogen), 1 M betaine, 5 mM DTT, 6 mM MgCl2, 1 uM TSO-primer*, 2.3 U RNase Inhibitor and 25 U Superscript II). The cDNA is amplified using 1x Kapa HiFi HotStart Readymix (Roche) and 0.1 uM UP-primer under following cycling conditions: 98°C for 3 min, 23 cycles [98°C 20 sec, 67°C 15 sec, 72°C 6 min] and final elongation at 72°C for 5 min. The amplified cDNA is purified using 1x volume of Sera-Mag SpeedBeads (GE Healthcare) and eluted in 12 ul nuclease free water. For library preparation 1.5 µl cDNA is desiccated and rehydrated in 1 ul Tagmentation mix (1x TTBL buffer, 0.1 ul Tagment DNA Enzyme; from TruePrep DNA Library Prep Kit V2 for Illumina; Vazyme) and tagmented (55°C, 5 min), followed by an index PCR with 1x concentrated KAPA Hifi HotStart Ready Mix and 0.7 µM dual indexing primers (72°C 3 min, 98°C 30 sec, 13 cycles [98°C 10 sec, 63°C 20 sec, 72°C 1 min], 72°C 5 min). After PCR, libraries are quantified with AccuBlue Broad range chemistry (Biotium), equimolarly pooled and purified twice with 0.9x volume Sera-Mag SpeedBeads.

text: embryo

text: normal

text: L40652_Track-80121: 103110; L40652_Track-79380: 181008

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40698_Track-79995: 291827; L40698_Track-79456: 582004

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40540_Track-80201: 195747; L40540_Track-79479: 393070

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP low

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40581_Track-80223: 141332; L40581_Track-79608: 347479

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40678_Track-79937: 162407; L40678_Track-79531: 325535

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

characteristic: description

text: embryo

text: normal

text: L40662_Track-80240: 87122; L40662_Track-79599: 205841

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP low

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

text: normal

text: L40638_Track-79922: 103624; L40638_Track-79346: 197473

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40624_Track-80175: 220426; L40624_Track-79339: 441574

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40646_Track-80144: 191418; L40646_Track-79323: 375732

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP low

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40677_Track-79973: 146781; L40677_Track-79505: 295425

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40628_Track-80221: 119022; L40628_Track-79605: 286160

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

characteristic: description

text: embryo

text: normal

text: L40666_Track-80166: 132533; L40666_Track-79309: 261861

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP low

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

text: normal

text: L40714_Track-79936: 219079; L40714_Track-79527: 436381

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40722_Track-80186: 281017; L40722_Track-79532: 557767

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

text: The Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40651_Track-80271: 191682; L40651_Track-79606: 461912

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP low

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: Protocols: hESC-derived pancreatic cells at Stage 4 Day 1 of the pancreatic differentiation protocol (Rezania et al., 2014; Petersen et al., 2017) were dissociated into single cells with TrypLE and resuspended into a FACS buffer containing 0.5% BSA and 2 mM EDTA (Thermo Fisher) in 1X PBS. The cells were index-sorted according to their GFP expression into 384-well plates on a BD FACSAria and snap-frozen. Cells are processed with SMARTseq-2 protocol miniaturized and modified based on Picelli et al. 2013 (doi: 10.1038/nmeth.2639). In short, cells are sorted in 0.5 µl 0.2% Triton X-100 solution with RNase Inhibitor into a 384-well plate, frozen at -80°C. After thawing, the cell lysate is mixed with 0.5 ul of a primer mix (containing 5 mM dNTP (Invitrogen), 0.5 uM dT-primer*, 1 U RNase Inhibitor (NEB)). RNA is denatured (3 minutes 72 °C), followed by reverse transcription (42 °C 90 min + 70°C 15 min) after adding 1.5 ul RT buffer mix (1x superscript II buffer (Invitrogen), 1 M betaine, 5 mM DTT, 6 mM MgCl2, 1 uM TSO-primer*, 2.3 U RNase Inhibitor and 25 U Superscript II). The cDNA is amplified using 1x Kapa HiFi HotStart Readymix (Roche) and 0.1 uM UP-primer under following cycling conditions: 98°C for 3 min, 23 cycles [98°C 20 sec, 67°C 15 sec, 72°C 6 min] and final elongation at 72°C for 5 min. The amplified cDNA is purified using 1x volume of Sera-Mag SpeedBeads (GE Healthcare) and eluted in 12 ul nuclease free water. For library preparation 1.5 µl cDNA is desiccated and rehydrated in 1 ul Tagmentation mix (1x TTBL buffer, 0.1 ul Tagment DNA Enzyme; from TruePrep DNA Library Prep Kit V2 for Illumina; Vazyme) and tagmented (55°C, 5 min), followed by an index PCR with 1x concentrated KAPA Hifi HotStart Ready Mix and 0.7 µM dual indexing primers (72°C 3 min, 98°C 30 sec, 13 cycles [98°C 10 sec, 63°C 20 sec, 72°C 1 min], 72°C 5 min). After PCR, libraries are quantified with AccuBlue Broad range chemistry (Biotium), equimolarly pooled and purified twice with 0.9x volume Sera-Mag SpeedBeads.

text: embryo

text: normal

text: L40539_Track-80214: 64461; L40539_Track-79603: 157493

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40537_Track-80204: 218021; L40537_Track-79511: 436147

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

characteristic: description

text: embryo

text: normal

text: L40541_Track-80261: 92271; L40541_Track-79600: 225279

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP low

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

text: normal

text: L40711_Track-79655: 7287

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40611_Track-79987: 193914; L40611_Track-79408: 389772

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40626_Track-79980: 246166; L40626_Track-79426: 491521

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP low

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40548_Track-80076: 278156; L40548_Track-79445: 552792

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40641_Track-79981: 142198; L40641_Track-79335: 282916

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

characteristic: description

text: embryo

text: normal

text: L40593_Track-80086: 132321; L40593_Track-79471: 267027

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP low

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

text: normal

text: L40635_Track-80256: 120977; L40635_Track-79584: 291236

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40670_Track-80033: 61906; L40670_Track-79300: 103844

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40549_Track-80194: 202861; L40549_Track-79553: 404950

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP low

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40634_Track-80209: 121102; L40634_Track-79593: 286205

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40644_Track-79656: 641854

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

characteristic: description

text: embryo

text: normal

text: L40688_Track-80104: 251560; L40688_Track-79299: 500310

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP low

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

text: normal

text: L40550_Track-80108: 229341; L40550_Track-79420: 459804

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: The Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40572_Track-80022: 231586; L40572_Track-79499: 467551

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: Protocols: hESC-derived pancreatic cells at Stage 4 Day 1 of the pancreatic differentiation protocol (Rezania et al., 2014; Petersen et al., 2017) were dissociated into single cells with TrypLE and resuspended into a FACS buffer containing 0.5% BSA and 2 mM EDTA (Thermo Fisher) in 1X PBS. The cells were index-sorted according to their GFP expression into 384-well plates on a BD FACSAria and snap-frozen. Cells are processed with SMARTseq-2 protocol miniaturized and modified based on Picelli et al. 2013 (doi: 10.1038/nmeth.2639). In short, cells are sorted in 0.5 µl 0.2% Triton X-100 solution with RNase Inhibitor into a 384-well plate, frozen at -80°C. After thawing, the cell lysate is mixed with 0.5 ul of a primer mix (containing 5 mM dNTP (Invitrogen), 0.5 uM dT-primer*, 1 U RNase Inhibitor (NEB)). RNA is denatured (3 minutes 72 °C), followed by reverse transcription (42 °C 90 min + 70°C 15 min) after adding 1.5 ul RT buffer mix (1x superscript II buffer (Invitrogen), 1 M betaine, 5 mM DTT, 6 mM MgCl2, 1 uM TSO-primer*, 2.3 U RNase Inhibitor and 25 U Superscript II). The cDNA is amplified using 1x Kapa HiFi HotStart Readymix (Roche) and 0.1 uM UP-primer under following cycling conditions: 98°C for 3 min, 23 cycles [98°C 20 sec, 67°C 15 sec, 72°C 6 min] and final elongation at 72°C for 5 min. The amplified cDNA is purified using 1x volume of Sera-Mag SpeedBeads (GE Healthcare) and eluted in 12 ul nuclease free water. For library preparation 1.5 µl cDNA is desiccated and rehydrated in 1 ul Tagmentation mix (1x TTBL buffer, 0.1 ul Tagment DNA Enzyme; from TruePrep DNA Library Prep Kit V2 for Illumina; Vazyme) and tagmented (55°C, 5 min), followed by an index PCR with 1x concentrated KAPA Hifi HotStart Ready Mix and 0.7 µM dual indexing primers (72°C 3 min, 98°C 30 sec, 13 cycles [98°C 10 sec, 63°C 20 sec, 72°C 1 min], 72°C 5 min). After PCR, libraries are quantified with AccuBlue Broad range chemistry (Biotium), equimolarly pooled and purified twice with 0.9x volume Sera-Mag SpeedBeads.

characteristic: description

text: embryo

text: normal

text: L40654_Track-80153: 155985; L40654_Track-79550: 307110

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP low

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40584_Track-80164: 196865; L40584_Track-79360: 393544

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40636_Track-80210: 34211; L40636_Track-79580: 73882

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

characteristic: description

text: embryo

text: normal

text: L40701_Track-80036: 179961; L40701_Track-79560: 363295

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP low

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

text: normal

text: L40563_Track-80149: 171458; L40563_Track-79449: 344575

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40623_Track-80264: 127462; L40623_Track-79636: 312088

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40653_Track-79974: 338517; L40653_Track-79413: 676192

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP low

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40622_Track-79965: 201999; L40622_Track-79341: 403590

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40669_Track-80181: 147761; L40669_Track-79329: 292792

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

characteristic: description

text: embryo

text: normal

text: L40710_Track-80139: 160471; L40710_Track-79278: 318416

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP low

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

text: normal

text: L40693_Track-80199: 96022; L40693_Track-79377: 193048

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40578_Track-79953: 323721; L40578_Track-79287: 649961

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40675_Track-80096: 280978; L40675_Track-79501: 560592

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP low

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40561_Track-79996: 176855; L40561_Track-79457: 353333

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: External Id

text: The Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40567_Track-80034: 246497; L40567_Track-79473: 491251

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: Protocols: hESC-derived pancreatic cells at Stage 4 Day 1 of the pancreatic differentiation protocol (Rezania et al., 2014; Petersen et al., 2017) were dissociated into single cells with TrypLE and resuspended into a FACS buffer containing 0.5% BSA and 2 mM EDTA (Thermo Fisher) in 1X PBS. The cells were index-sorted according to their GFP expression into 384-well plates on a BD FACSAria and snap-frozen. Cells are processed with SMARTseq-2 protocol miniaturized and modified based on Picelli et al. 2013 (doi: 10.1038/nmeth.2639). In short, cells are sorted in 0.5 µl 0.2% Triton X-100 solution with RNase Inhibitor into a 384-well plate, frozen at -80°C. After thawing, the cell lysate is mixed with 0.5 ul of a primer mix (containing 5 mM dNTP (Invitrogen), 0.5 uM dT-primer*, 1 U RNase Inhibitor (NEB)). RNA is denatured (3 minutes 72 °C), followed by reverse transcription (42 °C 90 min + 70°C 15 min) after adding 1.5 ul RT buffer mix (1x superscript II buffer (Invitrogen), 1 M betaine, 5 mM DTT, 6 mM MgCl2, 1 uM TSO-primer*, 2.3 U RNase Inhibitor and 25 U Superscript II). The cDNA is amplified using 1x Kapa HiFi HotStart Readymix (Roche) and 0.1 uM UP-primer under following cycling conditions: 98°C for 3 min, 23 cycles [98°C 20 sec, 67°C 15 sec, 72°C 6 min] and final elongation at 72°C for 5 min. The amplified cDNA is purified using 1x volume of Sera-Mag SpeedBeads (GE Healthcare) and eluted in 12 ul nuclease free water. For library preparation 1.5 µl cDNA is desiccated and rehydrated in 1 ul Tagmentation mix (1x TTBL buffer, 0.1 ul Tagment DNA Enzyme; from TruePrep DNA Library Prep Kit V2 for Illumina; Vazyme) and tagmented (55°C, 5 min), followed by an index PCR with 1x concentrated KAPA Hifi HotStart Ready Mix and 0.7 µM dual indexing primers (72°C 3 min, 98°C 30 sec, 13 cycles [98°C 10 sec, 63°C 20 sec, 72°C 1 min], 72°C 5 min). After PCR, libraries are quantified with AccuBlue Broad range chemistry (Biotium), equimolarly pooled and purified twice with 0.9x volume Sera-Mag SpeedBeads.

characteristic: description

text: embryo

text: normal

text: L40625_Track-80152: 232402; L40625_Track-79345: 469336

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP low

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

text: normal

text: L40559_Track-80251: 63591; L40559_Track-79618: 153786

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

characteristic: gfp mean

text: L40619_Track-79976: 223766; L40619_Track-79301: 453473

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40660_Track-80193: 231553; L40660_Track-79296: 459375

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP low

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40609_Track-80042: 170847; L40609_Track-79515: 342748

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

text: L40442_Track-80257: 74616; L40442_Track-79630: 180494

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP negative

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40445_Track-80232: 56047; L40445_Track-79629: 135843

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP negative

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40449_Track-79918: 124792; L40449_Track-79494: 249334

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP negative

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

text: L40472_Track-80088: 101263; L40472_Track-79571: 204256

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP negative

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40474_Track-80161: 137125; L40474_Track-79441: 273514

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP negative

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40477_Track-79988: 196927; L40477_Track-79548: 392241

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP negative

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

text: L40482_Track-79934: 240664; L40482_Track-79477: 484167

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP negative

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40491_Track-80123: 139615; L40491_Track-79379: 279666

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP negative

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40503_Track-80083: 150966; L40503_Track-79343: 303814

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP negative

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: The Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

text: L40505_Track-80226: 366405; L40505_Track-79642: 891429

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP negative

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: pancreatic cell

text: human

text: Protocols: hESC-derived pancreatic cells at Stage 4 Day 1 of the pancreatic differentiation protocol (Rezania et al., 2014; Petersen et al., 2017) were dissociated into single cells with TrypLE and resuspended into a FACS buffer containing 0.5% BSA and 2 mM EDTA (Thermo Fisher) in 1X PBS. The cells were index-sorted according to their GFP expression into 384-well plates on a BD FACSAria and snap-frozen. Cells are processed with SMARTseq-2 protocol miniaturized and modified based on Picelli et al. 2013 (doi: 10.1038/nmeth.2639). In short, cells are sorted in 0.5 µl 0.2% Triton X-100 solution with RNase Inhibitor into a 384-well plate, frozen at -80°C. After thawing, the cell lysate is mixed with 0.5 ul of a primer mix (containing 5 mM dNTP (Invitrogen), 0.5 uM dT-primer*, 1 U RNase Inhibitor (NEB)). RNA is denatured (3 minutes 72 °C), followed by reverse transcription (42 °C 90 min + 70°C 15 min) after adding 1.5 ul RT buffer mix (1x superscript II buffer (Invitrogen), 1 M betaine, 5 mM DTT, 6 mM MgCl2, 1 uM TSO-primer*, 2.3 U RNase Inhibitor and 25 U Superscript II). The cDNA is amplified using 1x Kapa HiFi HotStart Readymix (Roche) and 0.1 uM UP-primer under following cycling conditions: 98°C for 3 min, 23 cycles [98°C 20 sec, 67°C 15 sec, 72°C 6 min] and final elongation at 72°C for 5 min. The amplified cDNA is purified using 1x volume of Sera-Mag SpeedBeads (GE Healthcare) and eluted in 12 ul nuclease free water. For library preparation 1.5 µl cDNA is desiccated and rehydrated in 1 ul Tagmentation mix (1x TTBL buffer, 0.1 ul Tagment DNA Enzyme; from TruePrep DNA Library Prep Kit V2 for Illumina; Vazyme) and tagmented (55°C, 5 min), followed by an index PCR with 1x concentrated KAPA Hifi HotStart Ready Mix and 0.7 µM dual indexing primers (72°C 3 min, 98°C 30 sec, 13 cycles [98°C 10 sec, 63°C 20 sec, 72°C 1 min], 72°C 5 min). After PCR, libraries are quantified with AccuBlue Broad range chemistry (Biotium), equimolarly pooled and purified twice with 0.9x volume Sera-Mag SpeedBeads.

characteristic: description

text: embryo

text: normal

text: L40519_Track-80071: 135039; L40519_Track-79376: 270749

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP negative

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40521_Track-79983: 119470; L40521_Track-79321: 240040

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP negative

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

text: L40565_Track-80112: 170589; L40565_Track-79569: 343195

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40573_Track-80084: 181787; L40573_Track-79530: 363596

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP low

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40574_Track-79940: 203604; L40574_Track-79438: 410289

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

text: L40586_Track-80064: 121193; L40586_Track-79331: 240735

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40610_Track-80001: 104609; L40610_Track-79523: 208032

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP low

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40643_Track-80250: 149328; L40643_Track-79582: 361263

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

text: L40663_Track-80179: 178133; L40663_Track-79462: 347712

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40679_Track-80235: 45790; L40679_Track-79602: 111812

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP low

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40694_Track-79929: 261822; L40694_Track-79338: 522734

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

text: L40696_Track-79931: 167359; L40696_Track-79549: 334312

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP low

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: description

text: embryo

text: normal

text: L40727_Track-80212: 243255; L40727_Track-79576: 589774

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: GFP positive

characteristic: phenotype

text: plate 1

characteristic: plate id

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

text: ArrayExpress

text: pancreatic cell

text: human

characteristic: common name

text: embryo

text: normal

text: L40741_Track-80253: 70677; L40741_Track-79615: 169558

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: OK

characteristic: single cell well quality

text: Stage 4 Day 1

characteristic: time

characteristic: title

characteristic: well


characteristic: External Id

text: The Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pancreatic cell

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

text: L40754_Track-79969: 136712; L40754_Track-79519: 265743

characteristic: number of reads

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: GFP positive

text: plate 1

text: human embryonic stem cell

text: Homo sapiens

text: OK

text: Stage 4 Day 1