characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Food production

characteristic: environment (feature)

text: Food production

characteristic: environment (material)

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: food production metagenome

text: food production metagenome

characteristic: metagenomic source

text: Methanosarcina barkeri

characteristic: project name

characteristic: sample derived from

text: Methanosarcina barkeri

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR1562009_bin.77_CONCOCT_v1.1_MAG


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR1562009 of study SRP046072.

characteristic: description

text: Engineered

text: Food production

text: Food production

text: China

unit: DD

text: metagenome-assembled genome

text: food production metagenome

text: food production metagenome

text: uncultured Clostridia bacterium

characteristic: organism

text: Chinese liquor is produced by a solid-state fermentation process used for hundreds of years. The process is carried out in rectangular soil pits, whose walls have been layered with a fermentation mud. The age of these this layer is believed to influence final liquor quality and the microbes resident here form the inoculum for fermentation. Microbial diversity in these layers is poorly understood. This is the first report which describes a deep metagenomic sequencing based study to reveal the microbial diversity from 24 pits of varying ages, 20, 30, 50, 140, 220, 350, 400 and 440 years.

text: uncultured Clostridia bacterium

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: SRR1562009_bin.90_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Food production

characteristic: environment (feature)

text: Food production

characteristic: environment (material)

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: food production metagenome

text: food production metagenome

characteristic: metagenomic source

text: uncultured Marinilabiliaceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Marinilabiliaceae bacterium

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR1562009_bin.97_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR1562011 of study SRP046072.

characteristic: description

text: Engineered

text: Food production

text: Food production

text: China

text: metagenome-assembled genome

text: food production metagenome

text: food production metagenome

text: uncultured bacterium

characteristic: organism

text: uncultured bacterium

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: SRR1562011_bin.129_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Food production

characteristic: environment (feature)

text: Food production

characteristic: environment (material)

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: food production metagenome

text: food production metagenome

characteristic: metagenomic source

text: uncultured Methanobrevibacter sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Methanobrevibacter sp.

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR1562011_bin.39_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Engineered

text: Food production

text: Food production

text: China

text: metagenome-assembled genome

text: food production metagenome

text: food production metagenome

text: Lactobacillus acetotolerans

characteristic: organism

text: Lactobacillus acetotolerans

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: SRR1562011_bin.77_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR2753156 of study SRP065087.

text: Engineered

characteristic: environment (biome)

text: Food production

characteristic: environment (feature)

text: Food production

characteristic: environment (material)

text: Colombia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: food production metagenome

text: food production metagenome

characteristic: metagenomic source

text: uncultured Prevotella sp.

text: To characterize the microbial communities present in a cassava sour starch tank after 30 days of fermentation using high-throughput metagenome sequencing.

characteristic: project name

characteristic: sample derived from

text: uncultured Prevotella sp.

text: Illumina MiSeq

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR2753156_bin.14_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Engineered

text: Food production

text: Food production

text: Colombia

text: metagenome-assembled genome

text: food production metagenome

text: food production metagenome

text: uncultured Lactobacillus sp.

characteristic: organism

text: uncultured Lactobacillus sp.

text: Illumina MiSeq

text: multi-marker approach

text: Metagenome-assembled genome: SRR2753156_bin.3_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Food production

characteristic: environment (feature)

text: Food production

characteristic: environment (material)

text: Colombia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: food production metagenome

text: food production metagenome

characteristic: metagenomic source

text: uncultured Bacillales bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Bacillales bacterium

text: Illumina MiSeq

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR2753156_bin.51_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Engineered

text: Food production

text: Food production

text: Colombia

text: metagenome-assembled genome

text: food production metagenome

text: food production metagenome

text: uncultured Bifidobacterium sp.

characteristic: organism

text: uncultured Bifidobacterium sp.

text: Illumina MiSeq

text: multi-marker approach

text: Metagenome-assembled genome: SRR2753156_bin.73_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Food production

characteristic: environment (feature)

text: Food production

characteristic: environment (material)

text: Colombia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: food production metagenome

text: food production metagenome

characteristic: metagenomic source

text: uncultured Lactobacillus sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Lactobacillus sp.

text: Illumina MiSeq

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR2753156_bin.74_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Engineered

text: Food production

text: Food production

text: Colombia

text: metagenome-assembled genome

text: food production metagenome

text: food production metagenome

text: Lactobacillus amylolyticus

characteristic: organism

text: Lactobacillus amylolyticus

text: Illumina MiSeq

text: multi-marker approach

text: Metagenome-assembled genome: SRR2753156_bin.83_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR2753157 of study SRP065087.

text: Engineered

characteristic: environment (biome)

text: Food production

characteristic: environment (feature)

text: Food production

characteristic: environment (material)

text: Colombia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: food production metagenome

text: food production metagenome

characteristic: metagenomic source

text: uncultured Lactobacillus sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Lactobacillus sp.

text: Illumina MiSeq

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR2753157_bin.101_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Engineered

text: Food production

text: Food production

text: Colombia

text: metagenome-assembled genome

text: food production metagenome

text: food production metagenome

text: uncultured Eggerthellaceae bacterium

characteristic: organism

text: uncultured Eggerthellaceae bacterium

text: Illumina MiSeq

text: multi-marker approach

text: Metagenome-assembled genome: SRR2753157_bin.37_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Food production

characteristic: environment (feature)

text: Food production

characteristic: environment (material)

text: Colombia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: food production metagenome

text: food production metagenome

characteristic: metagenomic source

text: uncultured Veillonellaceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Veillonellaceae bacterium

text: Illumina MiSeq

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR2753157_bin.4_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Engineered

text: Food production

text: Food production

text: Colombia

text: metagenome-assembled genome

text: food production metagenome

text: food production metagenome

text: uncultured Bifidobacterium sp.

characteristic: organism

text: uncultured Bifidobacterium sp.

text: Illumina MiSeq

text: multi-marker approach

text: Metagenome-assembled genome: SRR2753157_bin.40_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Food production

characteristic: environment (feature)

text: Food production

characteristic: environment (material)

text: Colombia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: food production metagenome

text: food production metagenome

characteristic: metagenomic source

text: uncultured Pseudomonadaceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Pseudomonadaceae bacterium

text: Illumina MiSeq

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR2753157_bin.43_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Engineered

text: Food production

text: Food production

text: Colombia

text: metagenome-assembled genome

text: food production metagenome

text: food production metagenome

text: uncultured Lactobacillus sp.

characteristic: organism

text: uncultured Lactobacillus sp.

text: Illumina MiSeq

text: multi-marker approach

text: Metagenome-assembled genome: SRR2753157_bin.69_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Food production

characteristic: environment (feature)

text: Food production

characteristic: environment (material)

text: Colombia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: food production metagenome

text: food production metagenome

characteristic: metagenomic source

text: uncultured Lactobacillaceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Lactobacillaceae bacterium

text: Illumina MiSeq

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR2753157_bin.9_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR2753158 of study SRP065087.

characteristic: description

text: Engineered

text: Food production

text: Food production

text: Colombia

text: metagenome-assembled genome

text: food production metagenome

text: food production metagenome

text: uncultured Clostridia bacterium

characteristic: organism

text: uncultured Clostridia bacterium

text: Illumina MiSeq

text: multi-marker approach

text: Metagenome-assembled genome: SRR2753158_bin.65_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Food production

characteristic: environment (feature)

text: Food production

characteristic: environment (material)

text: Colombia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: food production metagenome

text: food production metagenome

characteristic: metagenomic source

text: uncultured Acetobacter sp.

text: To characterize the microbial communities present in a cassava sour starch tank after 30 days of fermentation using high-throughput metagenome sequencing.

characteristic: project name

characteristic: sample derived from

text: uncultured Acetobacter sp.

text: Illumina MiSeq

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR2753158_bin.98_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: not provided

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR3017090 of study SRP067433.

characteristic: description

text: Engineered

text: Food production

text: Food production

text: Australia

text: not provided

text: not provided

text: metagenome-assembled genome

text: food production metagenome

text: food production metagenome

text: uncultured Lactobacillus sp.

characteristic: organism

text:
Wine is a complex beverage, comprised of thousands of metabolites that are produced through the action of yeasts and bacteria in fermenting grape must. To ensure a robust and reliable fermentation, most commercial wines are produced via inoculation with large amounts of the major wine yeast, Saccharomyces cerevisiae. However, there is a growing trend towards the use of uninoculated or “wild” fermentations, in which the yeasts and bacteria that are naturally associated with the vineyard or winery perform the fermentation. The varied metabolic contributions of the numerous non-Saccharomyces species in this microbial community are thought to impart complexity and desirable taste and aroma attributes to wild ferments when compared to their inoculated counterparts.

In order the map the microflora of spontaneous fermentation, metagenomic techniques were used to characterise and monitor the progression of fungal species in several wild fermentations. Both amplicon-based ITS phylotyping and shotgun metagenomics were used to assess community structure, in addition to the isolation, sequencing and de novo assembly of the first reference genomes for several dominant wine-associated species.

text: uncultured Lactobacillus sp.

text: Illumina MiSeq

text: multi-marker approach

text: Metagenome-assembled genome: SRR3017090_bin.13_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR3017093 of study SRP067433.

text: Engineered

characteristic: environment (biome)

text: Food production

characteristic: environment (feature)

text: Food production

characteristic: environment (material)

text: Australia

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: food production metagenome

text: food production metagenome

characteristic: metagenomic source

text: Tatumella ptyseos

characteristic: project name

characteristic: sample derived from

text: Tatumella ptyseos

text: Illumina MiSeq

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR3017093_bin.57_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR5438753 of study SRP103682.

characteristic: description

text: Engineered

text: Food production

text: Food production

text: China

text: metagenome-assembled genome

text: food production metagenome

text: food production metagenome

text: Streptococcus pneumoniae

characteristic: organism

text: The theoretical basis for the industrial production of Kombucha

text: Streptococcus pneumoniae

text: HiSeq X Ten

text: multi-marker approach

text: Metagenome-assembled genome: SRR5438753_bin.16_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Food production

characteristic: environment (feature)

text: Food production

characteristic: environment (material)

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: food production metagenome

text: food production metagenome

characteristic: metagenomic source

text: Novacetimonas hansenii

characteristic: project name

characteristic: sample derived from

text: Novacetimonas hansenii

text: HiSeq X Ten

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR5438753_bin.23_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Engineered

text: Food production

text: Food production

text: China

text: metagenome-assembled genome

text: food production metagenome

text: food production metagenome

text: Komagataeibacter rhaeticus

characteristic: organism

text: Komagataeibacter rhaeticus

text: HiSeq X Ten

text: multi-marker approach

text: Metagenome-assembled genome: SRR5438753_bin.26_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Food production

characteristic: environment (feature)

text: Food production

characteristic: environment (material)

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: food production metagenome

text: food production metagenome

characteristic: metagenomic source

text: Komagataeibacter saccharivorans

characteristic: project name

characteristic: sample derived from

text: Komagataeibacter saccharivorans

text: HiSeq X Ten

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR5438753_bin.31_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Engineered

text: Food production

text: Food production

text: China

text: metagenome-assembled genome

text: food production metagenome

text: food production metagenome

text: Acetobacter aceti

characteristic: organism

text: Acetobacter aceti

text: HiSeq X Ten

text: multi-marker approach

text: Metagenome-assembled genome: SRR5438753_bin.35_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR5507318 of study SRP106447.

text: Engineered

characteristic: environment (biome)

text: Food production

characteristic: environment (feature)

text: Food production

characteristic: environment (material)

text: China

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: food production metagenome

text: food production metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

text: Matatranscriptome analysis of the active microbial community in aged pit mud

characteristic: project name

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: HiSeq X Ten

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR5507318_bin.11_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Engineered

text: Food production

text: Food production

text: China

text: not provided

text: not provided

text: metagenome-assembled genome

text: food production metagenome

text: food production metagenome

text: uncultured bacterium

characteristic: organism

text: uncultured bacterium

text: HiSeq X Ten

text: multi-marker approach

text: Metagenome-assembled genome: SRR5507318_bin.39_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Food production

characteristic: environment (feature)

text: Food production

characteristic: environment (material)

text: China

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: food production metagenome

text: food production metagenome

characteristic: metagenomic source

text: uncultured Bacteroidales bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Bacteroidales bacterium

text: HiSeq X Ten

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR5507318_bin.47_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Engineered

text: Food production

text: Food production

text: China

text: not provided

text: not provided

text: metagenome-assembled genome

text: food production metagenome

text: food production metagenome

text: uncultured Clostridia bacterium

characteristic: organism

text: uncultured Clostridia bacterium

text: HiSeq X Ten

text: multi-marker approach

text: Metagenome-assembled genome: SRR5507318_bin.50_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Engineered

characteristic: environment (biome)

text: Food production

characteristic: environment (feature)

text: Food production

characteristic: environment (material)

text: China

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: food production metagenome

text: food production metagenome

characteristic: metagenomic source

text: uncultured Methanomassiliicoccales archaeon

characteristic: project name

characteristic: sample derived from

text: HiSeq X Ten

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR5507318_bin.84_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1019366 of study ERP012016.

characteristic: description

text: Environmental

text: Terrestrial

text: Soil

text: USA

text: metagenome-assembled genome

text: soil metagenome

text: soil metagenome

text: uncultured Chloroflexi bacterium

characteristic: organism

text: Please see: Metagenomics Reveals Pervasive Bacterial Populations and Reduced Community Diversity across the Alaska Tundra Ecosystem. Johnston et al. 2016. Frontiers in Microbiology.doi: 10.3389/fmicb.2016.00579How soil microbial communities contrast with respect to taxonomic and functional composition within and between ecosystems remains an unresolved question that is central to predicting how global anthropogenic change will affect soil functioning and services. In particular, it remains unclear how small-scale observations of soil communities based on the typical volume sampled (1-2 g) are generalizable to ecosystem-scale responses and processes. This is especially relevant for remote, northern latitude soils, which are challenging to sample and are also thought to be more vulnerable to climate change compared to temperate soils. Here, we employed well-replicated shotgun metagenome and 16S rRNA gene amplicon sequencing to characterize community composition and metabolic potential in Alaskan tundra soils, combining our own datasets with those publically available from distant tundra and temperate grassland and agriculture habitats. We found that the abundance of many taxa and metabolic functions differed substantially between tundra soil metagenomes relative to those from temperate soils, and that a high degree of OTU-sharing exists between tundra locations. Tundra soils were an order of magnitude less complex than their temperate counterparts, allowing for near-complete coverage of microbial community richness (~92% breadth) by sequencing, and the recovery of 27 high-quality, almost complete (>80% completeness) population bins. These population bins, collectively, made up to ~10% of the metagenomic datasets, and represented diverse taxonomic groups and metabolic lifestyles tuned toward sulfur cycling, hydrogen metabolism, methanotrophy, and organic matter oxidation. Several population bins, including members of Acidobacteria, Actinobacteria, and Proteobacteria, were also present in geographically distant (~100-530 km apart) tundra habitats (full genome representation and up to 99.6% genome-derived average nucleotide identity). Collectively, our results revealed that Alaska tundra microbial communities are less diverse and more homogenous across spatial scales than previously anticipated, and provided DNA sequences of abundant populations and genes that would be relevant for future studies of the effects of environmental change on tundra ecosystems.

text: uncultured Chloroflexi bacterium

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: ERR1019366_bin.127_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes version 3.10.139

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Terrestrial

characteristic: environment (feature)

text: Soil

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: soil metagenome

text: soil metagenome

characteristic: metagenomic source

text: uncultured Burkholderiales bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Burkholderiales bacterium

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1019366_bin.130_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Environmental

text: Terrestrial

text: Soil

text: USA

text: metagenome-assembled genome

text: soil metagenome

text: soil metagenome

text: uncultured Spartobacteria bacterium

characteristic: organism

text: uncultured Spartobacteria bacterium

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: ERR1019366_bin.150_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes version 3.10.139

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Terrestrial

characteristic: environment (feature)

text: Soil

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: soil metagenome

text: soil metagenome

characteristic: metagenomic source

text: uncultured Solirubrobacterales bacterium

characteristic: project name

characteristic: sample derived from

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1019366_bin.184_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Environmental

text: Terrestrial

text: Soil

text: USA

text: metagenome-assembled genome

text: soil metagenome

text: soil metagenome

text: uncultured Caulobacteraceae bacterium

characteristic: organism

text: uncultured Caulobacteraceae bacterium

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: ERR1019366_bin.74_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes version 3.10.139

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Terrestrial

characteristic: environment (feature)

text: Soil

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: soil metagenome

text: soil metagenome

characteristic: metagenomic source

text: uncultured Acetobacteraceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Acetobacteraceae bacterium

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1019366_bin.85_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1022687 of study ERP012016.

characteristic: description

text: Environmental

text: Terrestrial

text: Soil

text: USA

text: metagenome-assembled genome

text: soil metagenome

text: soil metagenome

text: uncultured bacterium

characteristic: organism

text: uncultured bacterium

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: ERR1022687_bin.142_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes version 3.10.139

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Terrestrial

characteristic: environment (feature)

text: Soil

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: soil metagenome

text: soil metagenome

characteristic: metagenomic source

text: uncultured Methanobacterium sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Methanobacterium sp.

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1022687_bin.29_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Environmental

text: Terrestrial

text: Soil

text: USA

text: metagenome-assembled genome

text: soil metagenome

text: soil metagenome

text: uncultured Opitutaceae bacterium

characteristic: organism

text: uncultured Opitutaceae bacterium

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: ERR1022687_bin.54_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes version 3.10.139

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Terrestrial

characteristic: environment (feature)

text: Soil

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: soil metagenome

text: soil metagenome

characteristic: metagenomic source

text: uncultured Gemmatimonadetes bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Gemmatimonadetes bacterium

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1022687_bin.95_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1022692 of study ERP012016.

characteristic: description

text: Environmental

text: Terrestrial

text: Soil

text: USA

text: metagenome-assembled genome

text: soil metagenome

text: soil metagenome

characteristic: organism

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: ERR1022692_bin.78_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes version 3.10.139

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1034454 of study ERP012016.

text: Environmental

characteristic: environment (biome)

text: Terrestrial

characteristic: environment (feature)

text: Soil

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: soil metagenome

text: soil metagenome

characteristic: metagenomic source

text: uncultured Myxococcales bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Myxococcales bacterium

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1034454_bin.31_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Environmental

text: Terrestrial

text: Soil

text: USA

text: metagenome-assembled genome

text: soil metagenome

text: soil metagenome

text: uncultured Acidobacteria bacterium

characteristic: organism

text: Please see: Metagenomics Reveals Pervasive Bacterial Populations and Reduced Community Diversity across the Alaska Tundra Ecosystem. Johnston et al. 2016. Frontiers in Microbiology.doi: 10.3389/fmicb.2016.00579How soil microbial communities contrast with respect to taxonomic and functional composition within and between ecosystems remains an unresolved question that is central to predicting how global anthropogenic change will affect soil functioning and services. In particular, it remains unclear how small-scale observations of soil communities based on the typical volume sampled (1-2 g) are generalizable to ecosystem-scale responses and processes. This is especially relevant for remote, northern latitude soils, which are challenging to sample and are also thought to be more vulnerable to climate change compared to temperate soils. Here, we employed well-replicated shotgun metagenome and 16S rRNA gene amplicon sequencing to characterize community composition and metabolic potential in Alaskan tundra soils, combining our own datasets with those publically available from distant tundra and temperate grassland and agriculture habitats. We found that the abundance of many taxa and metabolic functions differed substantially between tundra soil metagenomes relative to those from temperate soils, and that a high degree of OTU-sharing exists between tundra locations. Tundra soils were an order of magnitude less complex than their temperate counterparts, allowing for near-complete coverage of microbial community richness (~92% breadth) by sequencing, and the recovery of 27 high-quality, almost complete (>80% completeness) population bins. These population bins, collectively, made up to ~10% of the metagenomic datasets, and represented diverse taxonomic groups and metabolic lifestyles tuned toward sulfur cycling, hydrogen metabolism, methanotrophy, and organic matter oxidation. Several population bins, including members of Acidobacteria, Actinobacteria, and Proteobacteria, were also present in geographically distant (~100-530 km apart) tundra habitats (full genome representation and up to 99.6% genome-derived average nucleotide identity). Collectively, our results revealed that Alaska tundra microbial communities are less diverse and more homogenous across spatial scales than previously anticipated, and provided DNA sequences of abundant populations and genes that would be relevant for future studies of the effects of environmental change on tundra ecosystems.

text: uncultured Acidobacteria bacterium

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: ERR1034454_bin.53_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes version 3.10.139

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1035437 of study ERP012016.

text: Environmental

characteristic: environment (biome)

text: Terrestrial

characteristic: environment (feature)

text: Soil

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: soil metagenome

text: soil metagenome

characteristic: metagenomic source

text: uncultured bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured bacterium

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1035437_bin.115_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Environmental

text: Terrestrial

text: Soil

text: USA

text: metagenome-assembled genome

text: soil metagenome

text: soil metagenome

text: uncultured Acidobacteria bacterium

characteristic: organism

text: uncultured Acidobacteria bacterium

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: ERR1035437_bin.117_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes version 3.10.139

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Terrestrial

characteristic: environment (feature)

text: Soil

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: soil metagenome

text: soil metagenome

characteristic: metagenomic source

text: uncultured Verrucomicrobiaceae bacterium

characteristic: project name

characteristic: sample derived from

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1035437_bin.123_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Environmental

text: Terrestrial

text: Soil

text: USA

text: metagenome-assembled genome

text: soil metagenome

text: soil metagenome

text: uncultured actinobacterium

characteristic: organism

text: uncultured actinobacterium

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: ERR1035437_bin.144_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes version 3.10.139

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Terrestrial

characteristic: environment (feature)

text: Soil

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: soil metagenome

text: soil metagenome

characteristic: metagenomic source

text: uncultured candidate division WPS-2 bacterium

characteristic: project name

characteristic: sample derived from

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1035437_bin.56_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Environmental

text: Terrestrial

text: Soil

text: USA

text: metagenome-assembled genome

text: soil metagenome

text: soil metagenome

text: uncultured Fibrobacteres bacterium

characteristic: organism

text: uncultured Fibrobacteres bacterium

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: ERR1035437_bin.85_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes version 3.10.139

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Terrestrial

characteristic: environment (feature)

text: Soil

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: soil metagenome

text: soil metagenome

characteristic: metagenomic source

text: uncultured proteobacterium

characteristic: project name

characteristic: sample derived from

text: uncultured proteobacterium

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1035437_bin.86_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1035438 of study ERP012016.

characteristic: description

text: Environmental

text: Terrestrial

text: Soil

text: USA

text: metagenome-assembled genome

text: soil metagenome

text: soil metagenome

text: uncultured archaeon

characteristic: organism

text: uncultured archaeon

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: ERR1035438_bin.115_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes version 3.10.139

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Terrestrial

characteristic: environment (feature)

text: Soil

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: soil metagenome

text: soil metagenome

characteristic: metagenomic source

text: uncultured Acidimicrobiaceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Acidimicrobiaceae bacterium

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1035438_bin.131_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Environmental

text: Terrestrial

text: Soil

text: USA

text: metagenome-assembled genome

text: soil metagenome

text: soil metagenome

text: uncultured Myxococcales bacterium

characteristic: organism

text: uncultured Myxococcales bacterium

text: Illumina HiSeq 2000

text: multi-marker approach

text: Metagenome-assembled genome: ERR1035438_bin.42_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: metaSPAdes version 3.10.139

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1039457 of study ERP012016.

text: Environmental

characteristic: environment (biome)

text: Terrestrial

characteristic: environment (feature)

text: Soil

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: soil metagenome

text: soil metagenome

characteristic: metagenomic source

text: uncultured Gallionella sp.

text: Please see: Metagenomics Reveals Pervasive Bacterial Populations and Reduced Community Diversity across the Alaska Tundra Ecosystem. Johnston et al. 2016. Frontiers in Microbiology.doi: 10.3389/fmicb.2016.00579How soil microbial communities contrast with respect to taxonomic and functional composition within and between ecosystems remains an unresolved question that is central to predicting how global anthropogenic change will affect soil functioning and services. In particular, it remains unclear how small-scale observations of soil communities based on the typical volume sampled (1-2 g) are generalizable to ecosystem-scale responses and processes. This is especially relevant for remote, northern latitude soils, which are challenging to sample and are also thought to be more vulnerable to climate change compared to temperate soils. Here, we employed well-replicated shotgun metagenome and 16S rRNA gene amplicon sequencing to characterize community composition and metabolic potential in Alaskan tundra soils, combining our own datasets with those publically available from distant tundra and temperate grassland and agriculture habitats. We found that the abundance of many taxa and metabolic functions differed substantially between tundra soil metagenomes relative to those from temperate soils, and that a high degree of OTU-sharing exists between tundra locations. Tundra soils were an order of magnitude less complex than their temperate counterparts, allowing for near-complete coverage of microbial community richness (~92% breadth) by sequencing, and the recovery of 27 high-quality, almost complete (>80% completeness) population bins. These population bins, collectively, made up to ~10% of the metagenomic datasets, and represented diverse taxonomic groups and metabolic lifestyles tuned toward sulfur cycling, hydrogen metabolism, methanotrophy, and organic matter oxidation. Several population bins, including members of Acidobacteria, Actinobacteria, and Proteobacteria, were also present in geographically distant (~100-530 km apart) tundra habitats (full genome representation and up to 99.6% genome-derived average nucleotide identity). Collectively, our results revealed that Alaska tundra microbial communities are less diverse and more homogenous across spatial scales than previously anticipated, and provided DNA sequences of abundant populations and genes that would be relevant for future studies of the effects of environmental change on tundra ecosystems.

characteristic: project name

characteristic: sample derived from

text: uncultured Gallionella sp.

text: Illumina HiSeq 2000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1039457_bin.10_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: unknown v0.0

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1654120 of study ERP017180.

characteristic: description

text: Environmental

text: Terrestrial

text: Soil

text: Belgium

text: metagenome-assembled genome

text: soil metagenome

text: soil metagenome

text: uncultured Acidobacteria bacterium

characteristic: organism

text: Within this study we used chitin as a soil amendment to influence plant growth, disease resistance and the rhizosphere microbiome. Lettuce plants were grown in potting soil for eight weeks or in potting soil amended with 2% chitin. The effect of the chitin addition was observed at the end of the experiment. Using amplicon sequencing, we already reported some first results, which can be found in: Debode et al. (2016) Chitin Mixed in Potting Soil Alters Lettuce Growth, the Survival of Zoonotic Bacteria on the Leaves and Associated Rhizosphere Microbiology. Frontiers in Microbiology 7.

text: uncultured Acidobacteria bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1654120_bin.28_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: unknown v0.0

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Terrestrial

characteristic: environment (feature)

text: Soil

characteristic: environment (material)

text: Belgium

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: soil metagenome

text: soil metagenome

characteristic: metagenomic source

text: uncultured Bacteroidetes bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Bacteroidetes bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1654120_bin.33_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: unknown v0.0

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Environmental

text: Terrestrial

text: Soil

text: Belgium

text: metagenome-assembled genome

text: soil metagenome

text: soil metagenome

text: uncultured Bordetella sp.

characteristic: organism

text: uncultured Bordetella sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1654120_bin.42_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: unknown v0.0

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Terrestrial

characteristic: environment (feature)

text: Soil

characteristic: environment (material)

text: Belgium

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: soil metagenome

text: soil metagenome

characteristic: metagenomic source

text: uncultured Phycisphaerales bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Phycisphaerales bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1654120_bin.66_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: unknown v0.0

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1654122 of study ERP017180.

characteristic: description

text: Environmental

text: Terrestrial

text: Soil

text: Belgium

text: metagenome-assembled genome

text: soil metagenome

text: soil metagenome

text: uncultured Schlesneria sp.

characteristic: organism

text: uncultured Schlesneria sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1654122_bin.0_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: unknown v0.0

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Terrestrial

characteristic: environment (feature)

text: Soil

characteristic: environment (material)

text: Belgium

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: soil metagenome

text: soil metagenome

characteristic: metagenomic source

text: uncultured Opitutae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Opitutae bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1654122_bin.11_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: unknown v0.0

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Environmental

text: Terrestrial

text: Soil

text: Belgium

text: metagenome-assembled genome

text: soil metagenome

text: soil metagenome

text: uncultured Paraburkholderia sp.

characteristic: organism

text: uncultured Paraburkholderia sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1654122_bin.3_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: unknown v0.0

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Terrestrial

characteristic: environment (feature)

text: Soil

characteristic: environment (material)

text: Belgium

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: soil metagenome

text: soil metagenome

characteristic: metagenomic source

text: uncultured Opitutaceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Opitutaceae bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1654122_bin.5_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: unknown v0.0

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1654126 of study ERP017180.

characteristic: description

text: Environmental

text: Terrestrial

text: Soil

text: Belgium

text: metagenome-assembled genome

text: soil metagenome

text: soil metagenome

text: uncultured Rhodospirillales bacterium

characteristic: organism

text: uncultured Rhodospirillales bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1654126_bin.17_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: unknown v0.0

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Terrestrial

characteristic: environment (feature)

text: Soil

characteristic: environment (material)

text: Belgium

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: soil metagenome

text: soil metagenome

characteristic: metagenomic source

text: uncultured Paraburkholderia sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Paraburkholderia sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1654126_bin.25_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: unknown v0.0

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Environmental

text: Terrestrial

text: Soil

text: Belgium

text: metagenome-assembled genome

text: soil metagenome

text: soil metagenome

text: uncultured Burkholderiales bacterium

characteristic: organism

text: uncultured Burkholderiales bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1654126_bin.32_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: unknown v0.0

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Terrestrial

characteristic: environment (feature)

text: Soil

characteristic: environment (material)

text: Belgium

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: soil metagenome

text: soil metagenome

characteristic: metagenomic source

text: uncultured Opitutaceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Opitutaceae bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1654126_bin.37_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: unknown v0.0

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Environmental

text: Terrestrial

text: Soil

text: Belgium

text: metagenome-assembled genome

text: soil metagenome

text: soil metagenome

text: uncultured Rhodanobacteraceae bacterium

characteristic: organism

text: uncultured Rhodanobacteraceae bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1654126_bin.46_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: unknown v0.0

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Terrestrial

characteristic: environment (feature)

text: Soil

characteristic: environment (material)

text: Belgium

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: soil metagenome

text: soil metagenome

characteristic: metagenomic source

text: uncultured Sphingobium sp.

text: Within this study we used chitin as a soil amendment to influence plant growth, disease resistance and the rhizosphere microbiome. Lettuce plants were grown in potting soil for eight weeks or in potting soil amended with 2% chitin. The effect of the chitin addition was observed at the end of the experiment. Using amplicon sequencing, we already reported some first results, which can be found in: Debode et al. (2016) Chitin Mixed in Potting Soil Alters Lettuce Growth, the Survival of Zoonotic Bacteria on the Leaves and Associated Rhizosphere Microbiology. Frontiers in Microbiology 7.

characteristic: project name

characteristic: sample derived from

text: uncultured Sphingobium sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1654126_bin.47_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: unknown v0.0

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Environmental

text: Terrestrial

text: Soil

text: Belgium

text: metagenome-assembled genome

text: soil metagenome

text: soil metagenome

text: uncultured Verrucomicrobia subdivision 3 bacterium

characteristic: organism

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR1654126_bin.50_CONCOCT_v1.1_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: unknown v0.0

text: Default

text: CONCOCT v1.1

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Environmental

characteristic: environment (biome)

text: Terrestrial

characteristic: environment (feature)

text: Soil

characteristic: environment (material)

text: Belgium

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: soil metagenome

text: soil metagenome

characteristic: metagenomic source

text: uncultured Armatimonadetes bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Armatimonadetes bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1654126_bin.64_CONCOCT_v1.1_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes version 3.10.139

characteristic: assembly software

text: Default

characteristic: binning parameters

text: CONCOCT v1.1

characteristic: binning software

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR671912 of study ERP008551.

characteristic: description

text: Environmental

text: Terrestrial

text: Soil

text: Australia

text: metagenome-assembled genome

text: soil metagenome

text: soil metagenome

text: uncultured actinobacterium

characteristic: organism

text: The samples in this study were collected as part of the BASE (Biomes of Australian Soil Environments) project, which is developing a database of Australian soil microbial diversity. The soil ecosystem is critical for ecosystem functioning, affecting human health and food supply, animal and plant health, edaphic factors and climate. The Base project has sampled soils from across a wide range of ecosystem types on the Australian continent and collected both contextual and DNA based data that are publically available and form a base line database of Australian soil microbial biodiversity. More information about BASE can be found at http://www.bioplatforms.com.au/special-initiatives/environment/soil-biodiversity. Collected here is Illumina shotgun sequencing data from these soils.

text: uncultured actinobacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: ERR671912_bin.21_CONCOCT_v1.1_MAG

characteristic: title