text: CNRS - CBM (UPR 4301)

characteristic: INSDC center name

text: public

characteristic: Submitter Id

text: ArrayExpress

text: baker's yeast

text: pCM185-Rho-NLS; Rrp6-Myc; mft1Δ

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

characteristic: title


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CNRS - CBM (UPR 4301)

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:CNRS - CBM (UPR 4301)

text: ArrayExpress

characteristic: broker name

text: baker's yeast

characteristic: common name

text: Protocols: S. cerevisiae cells were grown according to standard procedures at 25 °C in synthetic complete medium with bases and amino acids omitted as necessary for selection and with glucose (2%) as a carbon source. The pCM185 vector (CEN, TRP1) from Euroscarf was used as backbone to create the Doxycycline-regulated (Tet off) Rho expression construct (pCM185-Rho-NLS) (Mosrin-Huaman et al., 2009). Strains transformed by Rho expression vector were grown under repressing conditions (5 µg/mL of Doxycycline) at 25°C in YNB medium supplemented with SC-TRP (Synthetic Complete Supplement Mixture from MP Biomedicals) and glucose as carbon source (final concentration 2%). Strains transformed by Rho expression vector were grown under repressing conditions (5 µg/mL of Doxycycline) at 25°C in YNB medium supplemented with SC-TRP (Synthetic Complete Supplement Mixture from MP Biomedicals) and glucose as carbon source (final concentration 2%). To allow the expression of Rho, the yeast cells were grown the same medium but without Doxycycline. Growth was performed during 16 hours incubation at 25°C. DO was adjusting by dilution in order to obtain the same number of cell in each culture/condition. Proteins/DNA crosslinking was performed by adding 1.2 mL of Formaldehyde (37%, SIGMA) to the 45mL of each yeast culture and incubated 20 min at room temperature. Cross-linking reaction was stopped by adding 8mL of Glycine (2.5M) for 5 min and then put on ice. Cells were washed twice with cold TBS and twice in lysis buffer (50 mM Hepes pH 7.5, 140 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% Sodium deoxycholate and 1:100 Protease Inhibitor Cocktail from Promega). We then applied a centrifugation step at 13000 RPM, 5min at 5°C. After getting rid of the supernatant, pellet were resuspended in 1mL lysis buffer, and cells were break mechanically with glass beads (add directly to the samples). After recovering 1.2mL (=starting lysate), the cross-linked chromatin was sheared by sonication to reduce average fragment size to ~400 bp. The samples were centrifuged at 2500g and the supernatant recovered. 20 µL of supernatant genomic DNA was used as Input and the remaining was mixed with antibody solution (anti c-MYC sc-40X from Santa Cruz Biotechnology). Tubes were incubated overnight at 4°C, then protein G PLUS-agarose beads (Sigma) were added and rotated for 2 hours at 4°C followed by successive washing steps: 2 times with lysis buffer, 2 times with lysis buffer 2 (similar to lysis buffer but with 360 mM NaCl instead of 140 mM), 1 time with washing buffer and 1 time with TE. DNA was eluted twice at 65°C in elution buffer during 10 minutes. Samples (regardless of Input or IP) were reverse crosslinked at 65°C overnight. Proteinase K digestion of the eluates was performed prior to purification of the genomic DNA samples using the ThermoFisher GeneJET PCR Purification Kit. ChIP libraries preparation was performed following the NEBNext Ultra II DNA Library Prep instructions from NEB

characteristic: description

text: pCM185-Rho-NLS; Rrp6-Myc; mft1Δ

characteristic: genotype

text: Saccharomyces cerevisiae

characteristic: organism

text: Saccharomyces cerevisiae

characteristic: replicate

text: Saccharomyces cerevisiae

characteristic: strain


text: CNRS - CBM (UPR 4301)

characteristic: INSDC center name

text: public

characteristic: Submitter Id

text: ArrayExpress

text: baker's yeast

text: Protocols: S. cerevisiae cells were grown according to standard procedures at 25 °C in synthetic complete medium with bases and amino acids omitted as necessary for selection and with glucose (2%) as a carbon source. The pCM185 vector (CEN, TRP1) from Euroscarf was used as backbone to create the Doxycycline-regulated (Tet off) Rho expression construct (pCM185-Rho-NLS) (Mosrin-Huaman et al., 2009). Strains transformed by Rho expression vector were grown under repressing conditions (5 µg/mL of Doxycycline) at 25°C in YNB medium supplemented with SC-TRP (Synthetic Complete Supplement Mixture from MP Biomedicals) and glucose as carbon source (final concentration 2%). Strains transformed by Rho expression vector were grown under repressing conditions (5 µg/mL of Doxycycline) at 25°C in YNB medium supplemented with SC-TRP (Synthetic Complete Supplement Mixture from MP Biomedicals) and glucose as carbon source (final concentration 2%). DO was adjusting by dilution in order to obtain the same number of cell in each culture/condition. Proteins/DNA crosslinking was performed by adding 1.2 mL of Formaldehyde (37%, SIGMA) to the 45mL of each yeast culture and incubated 20 min at room temperature. Cross-linking reaction was stopped by adding 8mL of Glycine (2.5M) for 5 min and then put on ice. Cells were washed twice with cold TBS and twice in lysis buffer (50 mM Hepes pH 7.5, 140 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% Sodium deoxycholate and 1:100 Protease Inhibitor Cocktail from Promega). We then applied a centrifugation step at 13000 RPM, 5min at 5°C. After getting rid of the supernatant, pellet were resuspended in 1mL lysis buffer, and cells were break mechanically with glass beads (add directly to the samples). After recovering 1.2mL (=starting lysate), the cross-linked chromatin was sheared by sonication to reduce average fragment size to ~400 bp. The samples were centrifuged at 2500g and the supernatant recovered. 20 µL of supernatant genomic DNA was used as Input and the remaining was mixed with antibody solution (anti c-MYC sc-40X from Santa Cruz Biotechnology). Tubes were incubated overnight at 4°C, then protein G PLUS-agarose beads (Sigma) were added and rotated for 2 hours at 4°C followed by successive washing steps: 2 times with lysis buffer, 2 times with lysis buffer 2 (similar to lysis buffer but with 360 mM NaCl instead of 140 mM), 1 time with washing buffer and 1 time with TE. DNA was eluted twice at 65°C in elution buffer during 10 minutes. Samples (regardless of Input or IP) were reverse crosslinked at 65°C overnight. Proteinase K digestion of the eluates was performed prior to purification of the genomic DNA samples using the ThermoFisher GeneJET PCR Purification Kit. ChIP libraries preparation was performed following the NEBNext Ultra II DNA Library Prep instructions from NEB

text: pCM185-Rho-NLS; Rrp6-Myc; tho2Δ

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


characteristic: ENA-CHECKLIST

text: CNRS - CBM (UPR 4301)

text: public

text: ArrayExpress

text: baker's yeast

text: pCM185-Rho-NLS; Rrp6-Myc; tho2Δ

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: CNRS - CBM (UPR 4301)

text: public

text: E-MTAB-12378:IN_Rrp6-tho2-delta-C-1270_minus_Rho-1

text: ArrayExpress

text: baker's yeast

text: pCM185-Rho-NLS; Rrp6-Myc; tho2Δ 1271-1597

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


characteristic: External Id

text: CNRS - CBM (UPR 4301)

characteristic: INSDC first public

text: public

text: E-MTAB-12378:IN_Rrp6-tho2-delta-C-1270_minus_Rho-2

text: ArrayExpress

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: CNRS - CBM (UPR 4301)

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12378:IN_Rrp6-tho2-delta-C-1270_plus_Rho-1

text: ArrayExpress

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: CNRS - CBM (UPR 4301)

text: public

characteristic: SRA accession

text: E-MTAB-12378:IN_Rrp6-tho2-delta-C-1270_plus_Rho2

text: ArrayExpress

characteristic: broker name

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: CNRS - CBM (UPR 4301)

text: public

text: E-MTAB-12378:IN_Rrp6-tho2-delta-C-1407_minus_Rho-1

text: ArrayExpress

text: baker's yeast

characteristic: common name

characteristic: description

text: pCM185-Rho-NLS; Rrp6-Myc; tho2Δ 1408-1597

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: CNRS - CBM (UPR 4301)

text: public

text: E-MTAB-12378:IN_Rrp6-tho2-delta-C-1407_minus_Rho-2

text: ArrayExpress

text: baker's yeast

characteristic: genotype

text: Saccharomyces cerevisiae

characteristic: organism

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: CNRS - CBM (UPR 4301)

text: public

text: E-MTAB-12378:IN_Rrp6-tho2-delta-C-1407_plus_Rho-1

text: ArrayExpress

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

characteristic: replicate

text: Saccharomyces cerevisiae

characteristic: strain


text: CNRS - CBM (UPR 4301)

characteristic: INSDC center name

text: public

text: E-MTAB-12378:IN_Rrp6-tho2-delta-C-1407_plus_Rho2

characteristic: Submitter Id

text: ArrayExpress

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


characteristic: ENA-CHECKLIST

text: CNRS - CBM (UPR 4301)

text: public

text: ArrayExpress

text: baker's yeast

text: pCM185-Rho-NLS; Tho2-Myc

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: CNRS - CBM (UPR 4301)

text: public

text: ArrayExpress

text: baker's yeast

text: pCM185-Rho-NLS; Tho2-Myc

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


characteristic: External Id

text: CNRS - CBM (UPR 4301)

characteristic: INSDC first public

text: public

text: ArrayExpress

text: baker's yeast

text: pCM185-Rho-NLS; Hpr1-Myc

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: CNRS - CBM (UPR 4301)

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: baker's yeast

text: pCM185-Rho-NLS; Hpr1-Myc

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: CNRS - CBM (UPR 4301)

text: public

characteristic: SRA accession

text: ArrayExpress

characteristic: broker name

text: baker's yeast

text: pCM185-Rho-NLS; Mft1-Myc

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: CNRS - CBM (UPR 4301)

text: public

text: ArrayExpress

text: baker's yeast

characteristic: common name

characteristic: description

text: pCM185-Rho-NLS; Mft1-Myc

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: CNRS - CBM (UPR 4301)

text: public

text: ArrayExpress

text: baker's yeast

text: pCM185-Rho-NLS; Rrp6-Myc; mft1Δ

characteristic: genotype

text: Saccharomyces cerevisiae

characteristic: organism

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: CNRS - CBM (UPR 4301)

text: public

text: ArrayExpress

text: baker's yeast

text: pCM185-Rho-NLS; Rrp6-Myc; mft1Δ

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

characteristic: replicate

text: Saccharomyces cerevisiae

characteristic: strain


text: CNRS - CBM (UPR 4301)

characteristic: INSDC center name

text: public

characteristic: Submitter Id

text: ArrayExpress

text: baker's yeast

text: pCM185-Rho-NLS; Rrp6-Myc; tho2Δ

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


characteristic: ENA-CHECKLIST

text: CNRS - CBM (UPR 4301)

text: public

text: ArrayExpress

text: baker's yeast

text: pCM185-Rho-NLS; Rrp6-Myc; tho2Δ

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: CNRS - CBM (UPR 4301)

text: public

text: E-MTAB-12378:IP_Rrp6-tho2-delta-C-1270_minus_Rho-1

text: ArrayExpress

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


characteristic: External Id

text: CNRS - CBM (UPR 4301)

characteristic: INSDC first public

text: public

text: E-MTAB-12378:IP_Rrp6-tho2-delta-C-1270_minus_Rho-2

text: ArrayExpress

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: CNRS - CBM (UPR 4301)

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12378:IP_Rrp6-tho2-delta-C-1270_plus_Rho-1

text: ArrayExpress

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: CNRS - CBM (UPR 4301)

text: public

characteristic: SRA accession

text: E-MTAB-12378:IP_Rrp6-tho2-delta-C-1270_plus_Rho-2

text: ArrayExpress

characteristic: broker name

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: CNRS - CBM (UPR 4301)

text: public

text: E-MTAB-12378:IP_Rrp6-tho2-delta-C-1407_minus_Rho-1

text: ArrayExpress

text: baker's yeast

characteristic: common name

characteristic: description

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: CNRS - CBM (UPR 4301)

text: public

text: E-MTAB-12378:IP_Rrp6-tho2-delta-C-1407_minus_Rho-2

text: ArrayExpress

text: baker's yeast

characteristic: genotype

text: Saccharomyces cerevisiae

characteristic: organism

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: CNRS - CBM (UPR 4301)

text: public

text: E-MTAB-12378:IP_Rrp6-tho2-delta-C-1407_plus_Rho-1

text: ArrayExpress

text: baker's yeast

text: Protocols: S. cerevisiae cells were grown according to standard procedures at 25 °C in synthetic complete medium with bases and amino acids omitted as necessary for selection and with glucose (2%) as a carbon source. The pCM185 vector (CEN, TRP1) from Euroscarf was used as backbone to create the Doxycycline-regulated (Tet off) Rho expression construct (pCM185-Rho-NLS) (Mosrin-Huaman et al., 2009). Strains transformed by Rho expression vector were grown under repressing conditions (5 µg/mL of Doxycycline) at 25°C in YNB medium supplemented with SC-TRP (Synthetic Complete Supplement Mixture from MP Biomedicals) and glucose as carbon source (final concentration 2%). Strains transformed by Rho expression vector were grown under repressing conditions (5 µg/mL of Doxycycline) at 25°C in YNB medium supplemented with SC-TRP (Synthetic Complete Supplement Mixture from MP Biomedicals) and glucose as carbon source (final concentration 2%). To allow the expression of Rho, the yeast cells were grown the same medium but without Doxycycline. Growth was performed during 16 hours incubation at 25°C. DO was adjusting by dilution in order to obtain the same number of cell in each culture/condition. Proteins/DNA crosslinking was performed by adding 1.2 mL of Formaldehyde (37%, SIGMA) to the 45mL of each yeast culture and incubated 20 min at room temperature. Cross-linking reaction was stopped by adding 8mL of Glycine (2.5M) for 5 min and then put on ice. Cells were washed twice with cold TBS and twice in lysis buffer (50 mM Hepes pH 7.5, 140 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% Sodium deoxycholate and 1:100 Protease Inhibitor Cocktail from Promega). We then applied a centrifugation step at 13000 RPM, 5min at 5°C. After getting rid of the supernatant, pellet were resuspended in 1mL lysis buffer, and cells were break mechanically with glass beads (add directly to the samples). After recovering 1.2mL (=starting lysate), the cross-linked chromatin was sheared by sonication to reduce average fragment size to ~400 bp. The samples were centrifuged at 2500g and the supernatant recovered. 20 µL of supernatant genomic DNA was used as Input and the remaining was mixed with antibody solution (anti c-MYC sc-40X from Santa Cruz Biotechnology). Tubes were incubated overnight at 4°C, then protein G PLUS-agarose beads (Sigma) were added and rotated for 2 hours at 4°C followed by successive washing steps: 2 times with lysis buffer, 2 times with lysis buffer 2 (similar to lysis buffer but with 360 mM NaCl instead of 140 mM), 1 time with washing buffer and 1 time with TE. DNA was eluted twice at 65°C in elution buffer during 10 minutes. Samples (regardless of Input or IP) were reverse crosslinked at 65°C overnight. Proteinase K digestion of the eluates was performed prior to purification of the genomic DNA samples using the ThermoFisher GeneJET PCR Purification Kit. ChIP libraries preparation was performed following the NEBNext Ultra II DNA Library Prep instructions from NEB

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

characteristic: replicate

text: Saccharomyces cerevisiae

characteristic: strain


text: CNRS - CBM (UPR 4301)

characteristic: INSDC center name

text: public

text: E-MTAB-12378:IP_Rrp6-tho2-delta-C-1407_plus_Rho-2

characteristic: Submitter Id

text: ArrayExpress

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


characteristic: ENA-CHECKLIST

text: CNRS - CBM (UPR 4301)

text: public

text: ArrayExpress

text: baker's yeast

text: Protocols: S. cerevisiae cells were grown according to standard procedures at 25 °C in synthetic complete medium with bases and amino acids omitted as necessary for selection and with glucose (2%) as a carbon source. The pCM185 vector (CEN, TRP1) from Euroscarf was used as backbone to create the Doxycycline-regulated (Tet off) Rho expression construct (pCM185-Rho-NLS) (Mosrin-Huaman et al., 2009). Strains transformed by Rho expression vector were grown under repressing conditions (5 µg/mL of Doxycycline) at 25°C in YNB medium supplemented with SC-TRP (Synthetic Complete Supplement Mixture from MP Biomedicals) and glucose as carbon source (final concentration 2%). Strains transformed by Rho expression vector were grown under repressing conditions (5 µg/mL of Doxycycline) at 25°C in YNB medium supplemented with SC-TRP (Synthetic Complete Supplement Mixture from MP Biomedicals) and glucose as carbon source (final concentration 2%). DO was adjusting by dilution in order to obtain the same number of cell in each culture/condition. Proteins/DNA crosslinking was performed by adding 1.2 mL of Formaldehyde (37%, SIGMA) to the 45mL of each yeast culture and incubated 20 min at room temperature. Cross-linking reaction was stopped by adding 8mL of Glycine (2.5M) for 5 min and then put on ice. Cells were washed twice with cold TBS and twice in lysis buffer (50 mM Hepes pH 7.5, 140 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% Sodium deoxycholate and 1:100 Protease Inhibitor Cocktail from Promega). We then applied a centrifugation step at 13000 RPM, 5min at 5°C. After getting rid of the supernatant, pellet were resuspended in 1mL lysis buffer, and cells were break mechanically with glass beads (add directly to the samples). After recovering 1.2mL (=starting lysate), the cross-linked chromatin was sheared by sonication to reduce average fragment size to ~400 bp. The samples were centrifuged at 2500g and the supernatant recovered. 20 µL of supernatant genomic DNA was used as Input and the remaining was mixed with antibody solution (anti c-MYC sc-40X from Santa Cruz Biotechnology). Tubes were incubated overnight at 4°C, then protein G PLUS-agarose beads (Sigma) were added and rotated for 2 hours at 4°C followed by successive washing steps: 2 times with lysis buffer, 2 times with lysis buffer 2 (similar to lysis buffer but with 360 mM NaCl instead of 140 mM), 1 time with washing buffer and 1 time with TE. DNA was eluted twice at 65°C in elution buffer during 10 minutes. Samples (regardless of Input or IP) were reverse crosslinked at 65°C overnight. Proteinase K digestion of the eluates was performed prior to purification of the genomic DNA samples using the ThermoFisher GeneJET PCR Purification Kit. ChIP libraries preparation was performed following the NEBNext Ultra II DNA Library Prep instructions from NEB

text: pCM185-Rho-NLS; Tho2-Myc

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: CNRS - CBM (UPR 4301)

text: public

text: ArrayExpress

text: baker's yeast

text: pCM185-Rho-NLS; Tho2-Myc

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


characteristic: External Id

text: Innovent Biologics (Suzhou) Co. Ltd

characteristic: INSDC first public

text: public

tag: Namespace:Innovent Biologics (Suzhou) Co. Ltd

text: 6 to 8

unit: week

characteristic: age

text: ArrayExpress

text: leukocyte

text: house mouse

text: Protocols: Five days after the last dose (3mg/kg) of IgG, IL-2wt and IL-2nα mutein, MC38 tumor (four mice from each group) were dissected from mice and digested as above mentioned. Tumor-infiltrating leukocytes (TILs) were isolated by Percoll gradient and CD45+ leukocytes were enriched by CD45 (TIL) MicroBeads (Miltenyi, 130-110-618). Samples from the same group were labeled with different TotalSeq™ anti-mouse Hashtag (Biolegend) for 10x Genomics platform. Three samples from each group with higher cell viability were selected. 10,000 cells from each sample were mixed into a single sample and resuspended with a concentration of 1,000 cells/ul. Single-cell RNAseq library was constructed using the Chromium Next GEM Single Cell 3’ Reagent Kits v3.1. Single cell suspensions were first partitioned into nanoliter-scale Gel Beads-in-emulsion (GEMs). Full-length cDNA tagged with a 16nt 10x barcode and barcoded DNA from the cell surface protein Feature Barcode were generated within each individual GEM during RT reaction. Purified DNA after RT reaction was amplified for 11 cycles and size selection was used to separate the amplified cDNA molecules for 3ʹ Gene Expression and Cell Surface Protein library construction.

text: colon adenocarcinoma

characteristic: disease

characteristic: immunophenotype

text: pool of four mice

characteristic: individual

text: Mus musculus

text: Mus musculus

text: neoplasm

characteristic: sampling site

text: Mus musculus

text: female

characteristic: sex

characteristic: xenograft


text: Innovent Biologics (Suzhou) Co. Ltd

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 6 to 8

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: Protocols: Small tumors (60-80mm3) and large tumors (200-300mm3) with or without the indicated IL-2 treatment (treated for 3 days) were extracted for RNAseq analysis. Total RNA was isolated from each sample using the RNA mini kit (Qiagen, Germany). RNA quality was examined by gel electrophoresis and with Qubit (Thermo, Waltham, MA, USA). For RNA-sequencing, 1μg of total RNA was used for library construction. Sequencing libraries were generated using VAHTSTM Total RNA-seq (H/M/R) Library Prep Kit for Illumina.

characteristic: description

text: colon adenocarcinoma

text: Mus musculus

characteristic: organism

text: Mus musculus

text: colon

characteristic: organism part

text: neoplasm

text: Mus musculus

text: female

unit: cubic centimeter

characteristic: tumor size


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

tag: Namespace:ASST Grande Ospedale Metropolitano Niguarda

characteristic: Submitter Id

text: Foundation IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Milano

characteristic: collecting institution

text: Davide Mangioni

characteristic: collector name

text: endotracheal aspirate

text: Italy

characteristic: geographic location (country and/or sea)

text: Lombardy

characteristic: geographic location (region and locality)

characteristic: host age

text: Human

characteristic: host common name

text: Homo sapiens

characteristic: host scientific name

text: M

characteristic: host sex

characteristic: host subject id

text: Acinetobacter baumannii

text: Acinetobacter baumannii

characteristic: title


characteristic: ENA-CHECKLIST

text: ASST Grande Ospedale Metropolitano Niguarda

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Davide Mangioni

text: endotracheal aspirate

characteristic: description

text: Italy

text: Lombardy

text: Human

text: Homo sapiens

text: M

text: Acinetobacter baumannii

characteristic: organism

text: Acinetobacter baumannii


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: Davide Mangioni

text: bronchoalveolar lavage

text: Italy

text: Lombardy

text: Human

text: Homo sapiens

text: M

text: Acinetobacter baumannii

text: Acinetobacter baumannii


text: public

characteristic: collecting institution

text: Davide Mangioni

characteristic: collector name

text: rectal swab

text: Italy

characteristic: geographic location (country and/or sea)

text: Lombardy

characteristic: geographic location (region and locality)

characteristic: host age

text: Human

characteristic: host common name

text: Homo sapiens

text: F

text: Acinetobacter baumannii

text: Acinetobacter baumannii


characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

text: Davide Mangioni

text: endotracheal aspirate

text: Italy

text: Lombardy

text: Human

text: Homo sapiens

characteristic: host scientific name

text: F

characteristic: host sex

characteristic: host subject id

text: Acinetobacter baumannii

text: Acinetobacter baumannii

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Davide Mangioni

text: rectal swab

characteristic: description

text: Italy

text: Lombardy

text: Human

text: Homo sapiens

text: M

text: Acinetobacter baumannii

characteristic: organism

text: Acinetobacter baumannii


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

characteristic: collecting institution

text: Davide Mangioni

characteristic: collector name

text: pharyngeal swab

text: Italy

text: Lombardy

text: Human

text: Homo sapiens

text: M

text: Acinetobacter baumannii

text: Acinetobacter baumannii


characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

text: Davide Mangioni

text: pharyngeal swab

text: Italy

characteristic: geographic location (country and/or sea)

text: Lombardy

characteristic: geographic location (region and locality)

characteristic: host age

text: Human

characteristic: host common name

text: Homo sapiens

text: F

text: Acinetobacter baumannii

text: Acinetobacter baumannii


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: Davide Mangioni

text: Axilla swab

text: Italy

text: Lombardy

text: Human

text: Homo sapiens

characteristic: host scientific name

text: M

characteristic: host sex

characteristic: host subject id

text: Acinetobacter baumannii

text: Acinetobacter baumannii

characteristic: title


characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:BIOLOGY CENTRE ASCR, V.V.I., INSTITUTE OF HYDROBIO

text: freshwater lake [ENVO:00000021]

characteristic: broad-scale environmental context

characteristic: collection date

unit: m

characteristic: depth

text: Candidatus Planktophila isolated from the epilimnion of the Landstejn reservoir

characteristic: description

text: water

characteristic: environmental medium

text: Czech Republic

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mesotrophic lake [ENVO:01000775]

characteristic: local environmental context

text: Actinomycetia bacterium

characteristic: organism

characteristic: project name

text: Actinomycetia bacterium


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BIOLOGY CENTRE ASCR, V.V.I., INSTITUTE OF HYDROBIO

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: freshwater lake [ENVO:00000021]

text: Planktophila phage LanE43AprE7 (unclassified Caudoviricetes) isolated from the epilimnion of the Rimov reservoir

text: water

text: Czech Republic

characteristic: geographic location (country and/or sea)

text: mesotrophic lake [ENVO:01000775]

text: Caudovirales sp. ct2KA10

text: Caudovirales sp. ct2KA10


characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: freshwater lake [ENVO:00000021]

characteristic: broad-scale environmental context

text: Planktophila phage LanE43AprF5 (unclassified Caudoviricetes) isolated from the epilimnion of the Rimov reservoir

text: water

text: Czech Republic

text: mesotrophic lake [ENVO:01000775]

text: Caudovirales sp. ct2KA10

text: Caudovirales sp. ct2KA10


text: UNIVERSITY HOHENHEIM

text: public

tag: Namespace:UNIVERSITY HOHENHEIM

text: crop of laying hens

characteristic: description

text: Limosilactobacillus sp. BC6G17

text: Limosilactobacillus sp. BC6G17

text: isolate B002

characteristic: title


text: UNIVERSITY HOHENHEIM

text: public

text: ileum of laying hens

text: Ligilactobacillus aviarius

text: Ligilactobacillus aviarius

text: isolate B009


text: UNIVERSITY HOHENHEIM

text: public

text: ileum of laying hens

text: Limosilactobacillus reuteri

text: Limosilactobacillus reuteri

text: isolate B010


text: UNIVERSITY HOHENHEIM

text: public

text: ileum of laying hens

text: Limosilactobacillus sp. BIEM52

text: Limosilactobacillus sp. BIEM52

text: isolate B015


text: UNIVERSITY HOHENHEIM

text: public

text: crop of laying hens

text: Limosilactobacillus reuteri

text: Limosilactobacillus reuteri

text: isolate B031


text: UNIVERSITY HOHENHEIM

text: public

text: ileum of laying hens

text: Limosilactobacillus sp. BIEP23/BCEP10

text: Limosilactobacillus sp. BIEP23/BCEP10

text: isolate B032


text: UNIVERSITY HOHENHEIM

text: public

text: ileum of laying hens

text: Lactobacillus gallinarum

text: Lactobacillus gallinarum

text: isolate B035


text: UNIVERSITY HOHENHEIM

text: public

text: ileum of laying hens

text: Paraclostridium bifermentans

text: Paraclostridium bifermentans

text: isolate B040


text: UNIVERSITY HOHENHEIM

text: public

text: ileum of laying hens

text: Ligilactobacillus aviarius

text: Ligilactobacillus aviarius

text: isolate B056


text: UNIVERSITY HOHENHEIM

text: public

text: ileum of laying hens

text: Flavonifractor plautii

text: Flavonifractor plautii

text: isolate B058


text: UNIVERSITY HOHENHEIM

text: public

text: crop of laying hens

text: Clostridium sp. BCET17

text: Clostridium sp. BCET17

text: isolate B063


text: UNIVERSITY HOHENHEIM

text: public

text: crop of laying hens

text: Clostridium butyricum

text: Clostridium butyricum

text: isolate B067


text: UNIVERSITY HOHENHEIM

text: public

text: crop of laying hens

text: Ligilactobacillus saerimneri

text: Ligilactobacillus saerimneri

text: isolate B075


text: UNIVERSITY HOHENHEIM

text: public

text: ileum of laying hens

text: Ligilactobacillus saerimneri

text: Ligilactobacillus saerimneri

text: isolate B085


text: UNIVERSITY HOHENHEIM

text: public

text: crop of laying hens

text: Cutibacterium acnes

text: Cutibacterium acnes

text: isolate B094


text: UNIVERSITY HOHENHEIM

text: public

text: ileum of laying hens

text: Clostridium sp. CIES53

text: Clostridium sp. CIES53

text: isolate B095


text: UNIVERSITY HOHENHEIM

text: public

text: ileum of laying hens

text: Limosilactobacillus sp. CIEM62

text: Limosilactobacillus sp. CIEM62

text: isolate B114


text: UNIVERSITY HOHENHEIM

text: public

text: ileum of laying hens

text: Limosilactobacillus ingluviei

text: Limosilactobacillus ingluviei

text: isolate B104


text: UNIVERSITY HOHENHEIM

text: public

text: ileum of laying hens

text: Limosilactobacillus ingluviei

text: Limosilactobacillus ingluviei

text: isolate B107


text: UNIVERSITY HOHENHEIM

text: public

text: ileum of laying hens

text: Ligilactobacillus salivarius

text: Ligilactobacillus salivarius

text: isolate B108


text: UNIVERSITY HOHENHEIM

text: public

text: crop of laying hens

text: Cutibacterium acnes

text: Cutibacterium acnes

text: isolate B109


text: UNIVERSITY HOHENHEIM

text: public

text: crop of laying hens

text: Lactobacillus gallinarum

text: Lactobacillus gallinarum

text: isolate B110


text: UNIVERSITY HOHENHEIM

text: public

text: crop of laying hens

text: Clostridium butyricum

text: Clostridium butyricum

text: isolate B111


text: UNIVERSITY HOHENHEIM

text: public

text: crop of laying hens

text: Limosilactobacillus sp. BIEP23/BCEP10

text: Limosilactobacillus sp. BIEP23/BCEP10

text: isolate B116


text: UNIVERSITY HOHENHEIM

text: public

text: ileum of laying hens

text: Ligilactobacillus aviarius

text: Ligilactobacillus aviarius

text: isolate B118


text: UNIVERSITY HOHENHEIM

text: public

text: crop of laying hens

text: Limosilactobacillus ingluviei

text: Limosilactobacillus ingluviei

text: isolate B119


text: UNIVERSITY HOHENHEIM

text: public

text: ileum of broilers

text: Limosilactobacillus sp. BHUE2

text: Limosilactobacillus sp. BHUE2

text: isolate E2


text: UNIVERSITY HOHENHEIM

text: public

text: ileum of broilers

text: Streptococcus alactolyticus

text: Streptococcus alactolyticus

text: isolate I03


text: UNIVERSITY HOHENHEIM

text: public

text: ileum of broilers

text: Ligilactobacillus aviarius

text: Ligilactobacillus aviarius

text: isolate I11


text: UNIVERSITY HOHENHEIM

text: public

text: ileum of broilers

text: Enterococcus faecium

text: Enterococcus faecium

text: isolate I13


text: UNIVERSITY HOHENHEIM

text: public

text: ileum of broilers

text: Limosilactobacillus vaginalis

text: Limosilactobacillus vaginalis

text: isolate I18


text: UNIVERSITY HOHENHEIM

text: public

text: jejunum of broilers

text: Ligilactobacillus aviarius

text: Ligilactobacillus aviarius

text: isolate J01


text: UNIVERSITY HOHENHEIM

text: public

text: jejunum of broilers

text: Ligilactobacillus aviarius

text: Ligilactobacillus aviarius

text: isolate J03


text: UNIVERSITY HOHENHEIM

text: public

text: jejunum of broilers

text: Ligilactobacillus salivarius

text: Ligilactobacillus salivarius

text: isolate J06


text: UNIVERSITY HOHENHEIM

text: public

text: jejunum of broilers

text: Lactobacillus gallinarum

text: Lactobacillus gallinarum

text: isolate J07


text: UNIVERSITY HOHENHEIM

text: public

text: jejunum of broilers

text: Ligilactobacillus sp. DJ3G5/DJ4M19

text: Ligilactobacillus sp. DJ3G5/DJ4M19

text: isolate J11


text: UNIVERSITY HOHENHEIM

text: public

text: jejunum of broilers

text: Limosilactobacillus sp. DJ3M12

text: Limosilactobacillus sp. DJ3M12

text: isolate J21


text: UNIVERSITY HOHENHEIM

text: public

text: jejunum of broilers

text: Ligilactobacillus aviarius

text: Ligilactobacillus aviarius

text: isolate J25


text: UNIVERSITY HOHENHEIM

text: public

text: jejunum of broilers

text: Ligilactobacillus aviarius

text: Ligilactobacillus aviarius

text: isolate J28


text: UNIVERSITY HOHENHEIM

text: public

text: jejunum of broilers

text: Limosilactobacillus vaginalis

text: Limosilactobacillus vaginalis

text: isolate J29


text: UNIVERSITY HOHENHEIM

text: public

text: jejunum of broilers

text: Limosilactobacillus oris

text: Limosilactobacillus oris

text: isolate J30


text: UNIVERSITY HOHENHEIM

text: public

text: jejunum of broilers

text: Enterococcus faecium

text: Enterococcus faecium

text: isolate J32


text: UNIVERSITY HOHENHEIM

text: public

text: jejunum of broilers

text: Ligilactobacillus aviarius

text: Ligilactobacillus aviarius

text: isolate J35


text: NIAB

text: public

text: bulk isolate obtained from infected foliage of Beta vulgaris subsp. vulgaris

text: Cambridgeshire, UK

characteristic: geographic location (region and locality)

characteristic: isolate

text: sporangia on leaf surface

text: Peronospora farinosa

text: Peronospora farinosa

text: P. farinosa bulk isolate pf2


text: NIAB

text: public

text: bulk isolate obtained from infected foliage of Spinacia oleracea

text: The Netherlands

text: sporangia on leaf surface

text: Peronospora effusa

characteristic: organism

text: Peronospora effusa

text: P. effusa bulk isolate pe1


characteristic: ENA-CHECKLIST

text: NIAB

text: public

text: bulk isolate obtained from infected foliage of Brassica rapa

text: Cambridgeshire, UK

text: sporangia on leaf surface

text: Hyaloperonospora brassicae

text: Hyaloperonospora brassicae

text: H. brassicae bulk isolate hp1


characteristic: ENA-FIRST-PUBLIC

text: NIAB

text: public

text: bulk isolate obtained from infected foliage of Allium cepa

text: Cambridgeshire, UK

text: sporangia on leaf surface

text: Peronospora destructor

text: Peronospora destructor

text: P.destructor bulk isolate pd1


characteristic: ENA-LAST-UPDATE

text: Masaryk University, NCBR

text: public

tag: Namespace:Masaryk University, NCBR

text: ArrayExpress

characteristic: broker name

text: fission yeast

characteristic: common name

text: Protocols: All strains were cultivated into the mid-log phase (OD = 0.4 - 0.6) and incubated with 1 % formaldehyde for 15 min at room temperature to cross-link DNA-protein complexes. Glycine was added to a final concentration of 125 mM, and the incubation continued for 5 min. 5x10^8 cells were harvested and washed with 10 ml of ice-cold PBS. The yeast cell wall breakage was performed in 400 ul CHIP lysis buffer (50 mM HEPES, pH 7.5, 140 mM NaCl, 1 mM EDTA, 1 % Triton X-100, Complete EDTA-free protease inhibitor cocktail tablets, Roche) with half the volume of glass beads in 2 ml low binding tubes using FASTprep-24. The suspension was washed two times with CHIP lysis buffer (15 min, 4˚C, 15000 rpm), and 300 ul of the extract was sonicated with Bioruptor (Diagenode, 30 sec ON/30 sec OFF, High Power, 25 times) and clarified by centrifugation (15 min, 4˚C, 15000 rpm), resulting in an average DNA fragment size of 300-500 bp. 5 ul of the sonicated precleared extract was taken as an input control sample. Monoclonal anti-FLAG M2 antibody (F1804, Sigma) was diluted 1:150, incubated with precleared cell extract in 1.5 ml low binding tube for 2 h on ice, and precipitated over-night with Dynabeads protein G (Invitrogen). Precipitates were washed with 1 ml of CHIP lysis buffer, 1 ml of High Salt buffer (CHIP lysis buffer with 500 mM NaCl), 1 ml of Wash buffer (10 mM Tris-HCl at pH 8.0, 0.25 M LiCl, 0.5 % NP-40, 1 mM EDTA), and 1 ml of TE buffer (20 mM Tris-HCl at pH 8.0, 1 mM EDTA). Elution (50 mM Tris at pH 8, 0.1 % SDS, 10 mM EDTA) and de-crosslinking of samples were done overnight at 65˚C. DNA was purified using QIAquick PCR Purification Kit (Qiagen). The input DNA samples were tested for DNA fragmentation and determination of DNA concentration by the Fragment analyzer (Agilent). Input and immunoprecipitation (IP) samples with the best fragmentation and high concentration were used for the creation of NGS libraries (NEBNEXT ULTRA II DNA Library Prep kit, NEB).

text: Δada2::G418, Nse4-FLAG-HIS::KanR, ade6-704?, ade6-M216?, leu1-32, ura4-D18

characteristic: genotype

text: asynchronous culture

characteristic: growth condition

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: whole organism

characteristic: organism part

text: G5, biological replica

characteristic: replicate

text: Schizosaccharomyces pombe

characteristic: strain


characteristic: External Id

text: Masaryk University, NCBR

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: fission yeast

text: Δgcn5::G418, Nse4-FLAG-His::KanR, ura4-D18, ade6-M216?, leu1-32

text: asynchronous culture

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: whole organism

text: H3,biological replica

text: Schizosaccharomyces pombe


characteristic: ENA-CHECKLIST

text: Masaryk University, NCBR

text: public

text: ArrayExpress

text: fission yeast

characteristic: description

text: Nse4-FLAG-His KanR, leu1-32, ura4-D18

text: asynchronous culture

text: Schizosaccharomyces pombe

characteristic: organism

text: Schizosaccharomyces pombe

text: whole organism

text: G2, biological replica

text: Schizosaccharomyces pombe

characteristic: title


E-MTAB-12401:IN_NKG1 no tag

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Masaryk University, NCBR

text: public

text: E-MTAB-12401:IN_NKG1 no tag

text: ArrayExpress

characteristic: broker name

text: fission yeast

characteristic: common name

text: ura4-D18, ade6-704, leu1-32

text: asynchronous culture

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: whole organism

text: Schizosaccharomyces pombe

text: IN_NKG1 no tag


text: Masaryk University, NCBR

text: public

text: ArrayExpress

text: fission yeast

text: Δubp8::G418, Nse4-FLAG-HIS::KanR, ade6-704?, ade6-M216?, leu1-32, ura4-D18?

characteristic: genotype

text: asynchronous culture

characteristic: growth condition

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: whole organism

characteristic: organism part

text: H4, biological replica

characteristic: replicate

text: Schizosaccharomyces pombe


characteristic: External Id

text: Masaryk University, NCBR

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: fission yeast

text: asynchronous culture

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: whole organism

text: F5, biological replica

text: Schizosaccharomyces pombe

characteristic: strain


text: Masaryk University, NCBR

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: fission yeast

text: asynchronous culture

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: whole organism

text: H5, biological replica

text: Schizosaccharomyces pombe


characteristic: ENA-CHECKLIST

text: Masaryk University, NCBR

text: public

text: ArrayExpress

text: fission yeast

characteristic: description

text: asynchronous culture

text: Schizosaccharomyces pombe

characteristic: organism

text: Schizosaccharomyces pombe

text: whole organism

text: F3, biological replica

text: Schizosaccharomyces pombe

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Masaryk University, NCBR

text: public

text: ArrayExpress

characteristic: broker name

text: fission yeast

characteristic: common name

text: Nse4-FLAG-His KanR, leu1-32, ura4-D18

text: asynchronous culture

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: whole organism

text: F2, biological replica

text: Schizosaccharomyces pombe


E-MTAB-12401:IP_NKG1 no tag

text: Masaryk University, NCBR

text: public

text: E-MTAB-12401:IP_NKG1 no tag

text: ArrayExpress

text: fission yeast

text: ura4-D18, ade6-704, leu1-32

characteristic: genotype

text: asynchronous culture

characteristic: growth condition

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: whole organism

characteristic: organism part

characteristic: replicate

text: Schizosaccharomyces pombe

text: IP_NKG1 no tag


characteristic: External Id

text: Masaryk University, NCBR

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: fission yeast

text: asynchronous culture

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: whole organism

text: F4, biological replica

text: Schizosaccharomyces pombe

characteristic: strain


text: Masaryk University, NCBR

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: fission yeast

text: asynchronous culture

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: whole organism

text: G4, biological replica

text: Schizosaccharomyces pombe


text: Anopheles gambiae

characteristic: ArrayExpress-SPECIES

characteristic: ENA-CHECKLIST

text: SC

text: public

text: African malaria mosquito

text: Anopheles gambiae

characteristic: organism

text: Anopheles gambiae

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Severance Children's Hospital

text: public

tag: Namespace:Severance Children's Hospital

text: human

characteristic: common name

text: stool from biopsy proven nafld patients_1

characteristic: description

text: Homo sapiens

text: Homo sapiens


text: Severance Children's Hospital

text: public

text: human

text: stool from biopsy proven nafld patients_2

text: Homo sapiens

text: Homo sapiens


text: Severance Children's Hospital

text: public

text: human

text: stool from biopsy proven nafld patients_3

text: Homo sapiens

text: Homo sapiens


text: Severance Children's Hospital

text: public

text: human

text: stool from biopsy proven nafld patients_4

text: Homo sapiens

text: Homo sapiens


text: Severance Children's Hospital

text: public

text: human

text: stool from biopsy proven nafld patients_5

text: Homo sapiens

text: Homo sapiens


text: Severance Children's Hospital

text: public

text: human

text: stool from biopsy proven nafld patients_6

text: Homo sapiens

text: Homo sapiens


text: Severance Children's Hospital

text: public

text: human

text: stool from volunteer_1

text: Homo sapiens

text: Homo sapiens


text: Severance Children's Hospital

text: public

text: human

text: stool from volunteer_2

text: Homo sapiens

text: Homo sapiens


text: Severance Children's Hospital

text: public

text: human

text: stool from volunteer_3

text: Homo sapiens

text: Homo sapiens


text: Severance Children's Hospital

text: public

text: human

text: stool from volunteer_4

text: Homo sapiens

text: Homo sapiens


text: Severance Children's Hospital

text: public

text: human

text: stool from volunteer_5

text: Homo sapiens

text: Homo sapiens


text: Severance Children's Hospital

text: public

text: human

text: stool from volunteer_6

text: Homo sapiens

text: Homo sapiens


text: Severance Children's Hospital

text: public

text: house mouse

text: feces from specific pathogen free mouse_1

text: Mus musculus

text: Mus musculus


text: Severance Children's Hospital

text: public

text: house mouse

text: feces from specific pathogen free mouse_2

text: Mus musculus

text: Mus musculus


text: Severance Children's Hospital

text: public

text: house mouse

text: feces from specific pathogen free mouse_3

text: Mus musculus

text: Mus musculus


text: Severance Children's Hospital

text: public

text: house mouse

text: feces from specific pathogen free mouse_4

text: Mus musculus

text: Mus musculus


text: Severance Children's Hospital

text: public

text: house mouse

text: feces from specific pathogen free mouse_5

text: Mus musculus

text: Mus musculus


text: Severance Children's Hospital

text: public

text: house mouse

text: feces from fmt treated experimental nash mouse_1

text: Mus musculus

text: Mus musculus


text: Severance Children's Hospital

text: public

text: house mouse

text: feces from fmt treated experimental nash mouse_2

text: Mus musculus

text: Mus musculus


text: Severance Children's Hospital

text: public

text: house mouse

text: feces from fmt treated experimental nash mouse_3

text: Mus musculus

text: Mus musculus


text: Severance Children's Hospital

text: public

text: house mouse

text: feces from fmt treated experimental nash mouse_4

text: Mus musculus

text: Mus musculus


text: Severance Children's Hospital

text: public

text: house mouse

text: feces from fmt treated experimental nash mouse_5

text: Mus musculus

text: Mus musculus


text: Severance Children's Hospital

text: public

text: house mouse

text: feces from pbs treated experimental nash mouse_1

text: Mus musculus

text: Mus musculus


text: Severance Children's Hospital

text: public

text: house mouse

text: feces from pbs treated experimental nash mouse_2

text: Mus musculus

text: Mus musculus


text: Severance Children's Hospital

text: public

text: house mouse

text: feces from pbs treated experimental nash mouse_3

text: Mus musculus

text: Mus musculus


text: Severance Children's Hospital

text: public

text: house mouse

text: feces from pbs treated experimental nash mouse_4

text: Mus musculus

text: Mus musculus


text: Severance Children's Hospital

text: public

text: house mouse

text: feces from pbs treated experimental nash mouse_5

text: Mus musculus

text: Mus musculus


text: TONGJI

text: public

text: 18S rRNA genes of BAC filters

text: metagenome

text: metagenome


text: University of Bergen

text: public

tag: Namespace:University of Bergen

text: Synechococcus phage S-PM2 spontaneous deletion mutant sampled on day 39 from chemostat 1

text: Synechococcus phage S-PM2

text: Synechococcus phage S-PM2

text: S-PM2d day 39 chemostat 1


text: University of Bergen

text: public

text: Synechococcus phage S-PM2 spontaneous deletion mutant sampled on day 39 from chemostat 2

text: Synechococcus phage S-PM2

text: Synechococcus phage S-PM2

text: S-PM2d day 39 chemostat 2


text: University of Bergen

text: public

text: Synechococcus phage S-PM2 spontaneous deletion mutant sampled on day 39 from chemostat 3

text: Synechococcus phage S-PM2

text: Synechococcus phage S-PM2

text: S-PM2d day 39 chemostat 3


text: University of Bergen

text: public

text: Synechococcus phage S-PM2 spontaneous deletion mutant sampled on day 500 from chemostat 1

text: Synechococcus phage S-PM2

text: Synechococcus phage S-PM2

text: S-PM2d day 500 chemostat 1


text: University of Bergen

text: public

text: Synechococcus phage S-PM2 spontaneous deletion mutant sampled on day 500 from chemostat 2

text: Synechococcus phage S-PM2

text: Synechococcus phage S-PM2

text: S-PM2d day 500 chemostat 2


text: University of Bergen

text: public

text: Synechococcus phage S-PM2 spontaneous deletion mutant sampled on day 500 from chemostat 3

text: Synechococcus phage S-PM2

text: Synechococcus phage S-PM2

text: S-PM2d day 500 chemostat 3


text: Aarhus University

text: public

text: ena-SAMPLE-TAB-28-11-2022-13:19:10:907-79241

tag: Namespace:Aarhus University

text: Lotus japonicus

text: Lotus japonicus

text: Lotus japonicus WT Mock 10 dpi Replicate 1


text: Aarhus University

text: public

text: ena-SAMPLE-TAB-28-11-2022-13:19:10:907-79242

text: Lotus japonicus

text: Lotus japonicus

text: Lotus japonicus WT Mock 10 dpi Replicate 2


text: Aarhus University

text: public

text: ena-SAMPLE-TAB-28-11-2022-13:19:10:907-79243

text: Lotus japonicus

text: Lotus japonicus

text: Lotus japonicus WT M. loti R7A 10dpi Replicate 1


text: Aarhus University

text: public

text: ena-SAMPLE-TAB-28-11-2022-13:19:10:907-79244

text: Lotus japonicus

text: Lotus japonicus

text: Lotus japonicus WT M. loti R7A 10dpi Replicate 2


text: Aarhus University

text: public

text: ena-SAMPLE-TAB-28-11-2022-13:19:10:907-79245

text: Lotus japonicus

text: Lotus japonicus

text: Lotus japonicus WT Mock 5 dpi Replicate 1


text: Aarhus University

text: public

text: ena-SAMPLE-TAB-28-11-2022-13:19:10:907-79246

text: Lotus japonicus

text: Lotus japonicus

text: Lotus japonicus WT Mock 5 dpi Replicate 2


text: Aarhus University

text: public

text: ena-SAMPLE-TAB-28-11-2022-13:19:10:907-79247

text: Lotus japonicus

text: Lotus japonicus

text: Lotus japonicus WT M. loti R7A 5dpi Replicate 1


text: Aarhus University

text: public

text: ena-SAMPLE-TAB-28-11-2022-13:19:10:907-79248

text: Lotus japonicus

text: Lotus japonicus

text: Lotus japonicus WT M. loti R7A 5dpi Replicate 2


text: Aarhus University

text: public

text: ena-SAMPLE-TAB-28-11-2022-13:19:10:907-79249

text: Lotus japonicus

text: Lotus japonicus

text: Lotus japonicus cyclops Mock 5 dpi Replicate 1


text: Aarhus University

text: public

text: ena-SAMPLE-TAB-28-11-2022-13:19:10:907-79250

text: Lotus japonicus

text: Lotus japonicus

text: Lotus japonicus cyclops Mock 5 dpi Replicate 2


text: Aarhus University

text: public

text: ena-SAMPLE-TAB-28-11-2022-13:19:10:907-79251

text: Lotus japonicus

text: Lotus japonicus

text: Lotus japonicus cyclops M. loti R7A 5dpi Replicate 1


text: Aarhus University

text: public

text: ena-SAMPLE-TAB-28-11-2022-13:19:10:908-79252

text: Lotus japonicus

text: Lotus japonicus

text: Lotus japonicus cyclops M. loti R7A 5dpi Replicate 2


text: UNIVERSITY OF HOHENHEIM

text: public

tag: Namespace:UNIVERSITY OF HOHENHEIM

text: murine-gut

characteristic: broad-scale environmental context

characteristic: collection date

text: house mouse

text: fecal samples

characteristic: environmental medium

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: SPF laboratory mice

characteristic: local environmental context

text: Mus musculus

text: NLRC5 KO murine study

characteristic: project name

text: Mus musculus


characteristic: External Id

text: University of Helsinki

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:University of Helsinki

characteristic: Submitter Id

text: Whole genome sequence of mecC-MRSA isolated from a hedgehog

text: Finland

text: Erinaceus europaeus

text: Staphylococcus aureus

text: Staphylococcus aureus

text: MRSA in Finnish hedgehogs


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: University of Helsinki

text: public

text: Whole genome sequence of mecA-MRSA isolated from a hedgehog

text: Finland

text: Erinaceus europaeus

text: Staphylococcus aureus

characteristic: organism

text: Staphylococcus aureus

text: MRSA in Finnish hedgehogs

characteristic: title


text: Bladder

characteristic: ArrayExpress-PHENOTYPE

text: female

characteristic: ArrayExpress-SEX

text: Felis catus

characteristic: ArrayExpress-SPECIES

text: Female

characteristic: GENDER

text: SC

text: public

text: Bladder

characteristic: PHENOTYPE

text: domestic cat

characteristic: common name

text: Felis catus

text: Felis catus


text: Liver

text: female

text: Felis catus

characteristic: External Id

text: Female

text: SC

characteristic: INSDC center name

text: public

text: Liver

text: domestic cat

text: Felis catus

text: Felis catus


text: Bladder

text: male

text: Felis catus

text: Male

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Bladder

text: domestic cat

text: Felis catus

text: Felis catus


text: Liver

text: male

text: Felis catus

text: Male

text: SC

text: public

characteristic: INSDC status

text: Liver

characteristic: SRA accession

text: domestic cat

text: Felis catus

text: Felis catus


text: Lymph node

text: male

text: Felis catus

characteristic: ENA-CHECKLIST

text: Male

text: SC

text: public

text: Lymph node

characteristic: Submitter Id

text: domestic cat

text: Felis catus

text: Felis catus


text: Lymph node

text: female

text: Felis catus

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Female

text: SC

text: public

text: Lymph node

text: domestic cat

text: Felis catus

text: Felis catus


text: Pancreas

text: female

text: Felis catus

text: Female

text: SC

text: public

text: Pancreas

text: domestic cat

text: Felis catus

characteristic: organism

text: Felis catus

characteristic: title


text: Urethra

characteristic: ArrayExpress-PHENOTYPE

text: female

characteristic: ArrayExpress-SEX

text: Felis catus

text: Female

text: SC

text: public

text: Urethra

text: domestic cat

text: Felis catus

text: Felis catus


text: Lung

text: female

text: Felis catus

characteristic: ArrayExpress-SPECIES

text: Female

characteristic: GENDER

text: SC

text: public

text: Lung

text: domestic cat

text: Felis catus

text: Felis catus


text: Parathyroid

text: female

text: Felis catus

text: Female

text: SC

text: public

text: Parathyroid

characteristic: PHENOTYPE

text: domestic cat

characteristic: common name

text: Felis catus

text: Felis catus


text: Urethra

text: male

text: Felis catus

characteristic: External Id

text: Male

text: SC

characteristic: INSDC center name

text: public

text: Urethra

text: domestic cat

text: Felis catus

text: Felis catus


text: Kidney

text: male

text: Felis catus

text: Male

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Kidney

text: domestic cat

text: Felis catus

text: Felis catus


text: Heart

text: male

text: Felis catus

text: Male

text: SC

text: public

characteristic: INSDC status

text: Heart

characteristic: SRA accession

text: domestic cat

text: Felis catus

text: Felis catus


text: Vulva

text: female

text: Felis catus

characteristic: ENA-CHECKLIST

text: Female

text: SC

text: public

text: Vulva

characteristic: Submitter Id

text: domestic cat

text: Felis catus

text: Felis catus


text: Ureter

text: male

text: Felis catus

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Male

text: SC

text: public

text: Ureter

text: domestic cat

text: Felis catus

text: Felis catus


text: Bladder

text: male

text: <html>Felis catus </html>

text: Male

text: SC

text: public

text: Bladder

text: domestic cat

text: Felis catus

characteristic: organism

text: Felis catus

characteristic: title


text: Liver

characteristic: ArrayExpress-PHENOTYPE

text: male

characteristic: ArrayExpress-SEX

text: <html>Felis catus </html>

text: Male

text: SC

text: public

text: Liver

text: domestic cat

text: Felis catus

text: Felis catus


text: Bladder

text: female

text: <html>Felis catus </html>

characteristic: ArrayExpress-SPECIES

text: Female

characteristic: GENDER

text: SC

text: public

text: Bladder

text: domestic cat

text: Felis catus

text: Felis catus


text: KAUST

text: public

text: human

characteristic: common name

text: uninfected patients, nasopharyngeal swab

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: Healthy control


characteristic: External Id

text: KAUST

characteristic: INSDC center name

text: public

text: human

text: patients infected with SARS-CoV-2 virus carrying the N protein with RG at positions 203/204, nasopharyngeal swab

text: Homo sapiens

text: Homo sapiens

text: RG


text: KAUST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: human

text: patients infected with SARS-CoV-2 virus carrying the N protein with KR at positions 203/204, nasopharyngeal swab

text: Homo sapiens

text: Homo sapiens

text: KR


text: KAUST

text: public

characteristic: INSDC status

characteristic: SRA accession

text: human

text: HEK293T cells infected with VLPs carrying D614G S protein and WT N protein

text: Homo sapiens

text: Homo sapiens


characteristic: ENA-CHECKLIST

text: KAUST

text: public

characteristic: Submitter Id

text: human

text: HEK293T cells, uninfected control

text: Homo sapiens

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: KAUST

text: public

text: human

text: HEK293T cells infected with VLPs carrying D614G S protein and R203K/G204R N protein

text: Homo sapiens

text: Homo sapiens


text: KAUST

text: public

text: human

text: HEK293T cells infected with VLPs carrying WT S protein and R203K/G204R N protein

text: Homo sapiens

characteristic: organism

text: Homo sapiens

characteristic: title


text: KAUST

text: public

text: human

text: HEK293T cells infected with VLPs carrying WT S protein and WT N protein

text: Homo sapiens

text: Homo sapiens


text: Korea Food Research Institute

text: public

tag: Namespace:Korea Food Research Institute

text: human oral metagenome

text: human oral metagenome


text: Shanghai Tenth People's Hospital

text: public

tag: Namespace:Shanghai Tenth People's Hospital

text: ArrayExpress

characteristic: broker name

text: Protocols: Strains were maintained at -80˚C. Strains were streaked on YPD plates. The plates were incubated at 37˚C overnight. Then several colonies were picked up and suspended in DDW. Cell density was adjusted to 10,000 cells/ml, then 100µl of cell suspension was plated on YPD plates. The plates were incubated at 37˚C overnight. Colonies were picked up and suspended in DDW, then cell pellets were obtained by centrifuging at highest speed for 1min. We used the phenol-chloroform method to extract Genomic DNA. Cell pellets were suspended in 200µl of lysis buffer, add 100µl of acid washed glass beads, and 0,2ml phenol:chloroform:isoamyl alcohol (25:24:1). Vortex for 30min at 4˚C. Add 200µl TE pH7.5. Centrifuge for 5min at maximum speed. Transfer aqueous layer to a new 1.5ml EP tube. Add 1ml 100% ethanol. Precipitate 1h at -20˚C. Pellet DNA by centrifuging for 10min at maximum speed. Discard the supernatant. Add 400µl TE pH 7.5. Add 3ul of RNase A (10mg/ml). Incubate at 37˚C for 30min. Add 10µl of 3M NaOAc and 1ml of 100% ethanol. Mix by inversion. Precipitate 30min at -20˚C. Pellet DNA by centrifuging for 10min at maximum speed. Discard supernatant. Air-dry the pellet overnight. Resuspend in 50µl water. 1µg Genomic DNA was randomly fragmented by Covaris, followed by fragments selection by Agencourt AMPure XP-Medium kit to an average size of 200~400 bp. Selected fragments were end repaired and 3\adenylate, then the adaptors were ligated to the end of these 3\adenylate fragments. The products were amplified by PCR and purified by the Agencourt AMPure XP-Medium kit. The purified double stranded PCR products were heat denatured to single strand, then circularised by the splint oligo sequence. The single strand circle DNA (ssCirDNA) were formatted as the final library and qualified by QC.

characteristic: genotype

text: YPD plate

characteristic: growth condition

text: Cryptococcus neoformans var. grubii H99

text: Cryptococcus neoformans var. grubii H99

text: whole organism

characteristic: organism part

text: Cryptococcus neoformans var. grubii H99

characteristic: strain


characteristic: External Id

text: UNIVERSITY OF COPENHAGEN

text: public

tag: Namespace:UNIVERSITY OF COPENHAGEN

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: Salmon digestive system

characteristic: broad-scale environmental context

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR9360679 of study ERP136460.

characteristic: description

text: Gut

characteristic: environmental medium

text: Norway

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: fish gut metagenome

text: Digestive tube

characteristic: local environmental context

text: fish gut metagenome

characteristic: metagenomic source

text: Pseudomonas aeruginosa

text: HoloFood Salmon - Trial C Metagenome

characteristic: project name

characteristic: sample derived from

text: Pseudomonas aeruginosa

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas;s__Pseudomonas aeruginosa

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR9360679_bin.1_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF COPENHAGEN

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: Salmon digestive system

characteristic: broad-scale environmental context

text: EMG broker account, EMBL-EBI

characteristic: broker name

characteristic: collection date

text: CheckM

text: This sample represents a Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR9360692 of study ERP136460.

text: Gut

text: Norway

text: metagenome-assembled genome

text: fish gut metagenome

text: Digestive tube

text: fish gut metagenome

text: Aeromonas sobria

characteristic: organism

text: HoloFood Salmon - Trial C Metagenome

text: Aeromonas sobria

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Aeromonadales;f__Aeromonadaceae;g__Aeromonas;s__Aeromonas sobria

text: multi-marker approach

text: Metagenome-assembled genome: ERR9360692_bin.1_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF COPENHAGEN

text: public

text: MetaWRAP v1.3

text: Salmon digestive system

text: EMG broker account, EMBL-EBI

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR9360711 of study ERP136460.

characteristic: description

text: Gut

characteristic: environmental medium

text: Norway

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: fish gut metagenome

text: Digestive tube

characteristic: local environmental context

text: fish gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

text: HoloFood Salmon - Trial C Metagenome

characteristic: project name

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Ruminococcaceae;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR9360711_bin.1_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IOA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Pool of 156 samples

text: United Kingdom

text: Mytilus edulis

characteristic: organism

text: yes; 154

characteristic: pool

text: Mytilus edulis

text: Pool library 4

characteristic: title


text: public

tag: Namespace:Laboratory of Plant Physiology, Plant Sciences Group, Wageningen University& Research

unit: day

characteristic: age

text: ArrayExpress

characteristic: broker name

text: Protocols: Root materials were harvested by razor blades from S. parvula seedlings grown for 4 days on ½ MS agar plates and transferred on mesh to new medium supplemented with 0 mM or 175 mM NaCl for 0 h, 3 h, 24 h or 48 h. Seeds of Schrenkiella parvula (Lake Tuz ecotype) were stratified in dark at 4°C for 7 days and were surface-sterilized in 30% (v/v) sodium hypochlorite and washed 4 times with H2O. Seeds were geminated on nylon mesh strips on ½-strength Murashige-Skoog (½ MS) medium including vitamins (Duchefa), supplemented with 0.1% 2-(N-morpholino) ethanesulfonic acid (MES, Duchefa), 1% Daishin agar (Duchefa) and pH was adjusted with KOH to 5.75. Plates were placed at 70° racks in the growth chamber with 16 h light:8 h dark, 130 μmol m-2 s-1 LED light, 21°C and 70% relative humidity. Samples were frozen in liquid nitrogen and ground before RNA extraction. Total RNA was isolated using NZY Total RNA Isolation kit (NZYtech). RNA library preparation was conducted by Novogene Co., LTD (United Kingdom)

characteristic: description

text: seedling development stage

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: MS agar

characteristic: growth condition

text: Schrenkiella parvula

text: Schrenkiella parvula

text: root

characteristic: organism part

text: Schrenkiella parvula


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Laboratory of Plant Physiology, Plant Sciences Group, Wageningen University& Research

characteristic: INSDC center name

text: public

text: ArrayExpress

text: seedling development stage

text: normal

text: wild type genotype

text: MS agar

text: Schrenkiella parvula

text: Schrenkiella parvula

text: root

text: Schrenkiella parvula


text: BIOLOGY CENTRE ASCR, V.V.I., INSTITUTE OF HYDROBIO

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: One or multiple fragments, totaling ≥ 90% of the expected genome or replicon sequence or predicted complete

characteristic: UViG assembly quality

text: MEGAHIT

characteristic: assembly software

text: Freshwater lake

characteristic: broad-scale environmental context

characteristic: collection date

text: independent sequence (UViG)

characteristic: detection type

text: environmental material

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Freshwater lake

text: Freshwater lake

characteristic: local environmental context

text: freshwater metagenome

characteristic: metagenomic source

text: uncultured Caudovirales phage

text: undetermined

characteristic: predicted genome structure

text: dsDNA

characteristic: predicted genome type

text: High resolution sampling of freshwater reservoir in Czech Republic (PRJEB52406)

characteristic: project name

characteristic: sample derived from

text: uncultured Caudovirales phage

text: metagenome (not viral targeted)

characteristic: source of UViGs

text: VIBRANT; default settings

characteristic: viral identification software


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: UViG assembly quality

text: MEGAHIT;

text: Freshwater lake

characteristic: description

text: independent sequence (UViG)

text: environmental material

text: Czech Republic

text: Freshwater lake

text: Freshwater lake

text: freshwater metagenome

text: uncultured Caudovirales phage

characteristic: organism

text: undetermined

text: dsDNA

text: uncultured Caudovirales phage

text: metagenome (not viral targeted)

characteristic: title

text: VIBRANT; default settings


text: public

text: MEGAHIT; kmer set 49,69,89,109,129,216

characteristic: assembly software

text: Freshwater lake

characteristic: broad-scale environmental context

characteristic: collection date

text: independent sequence (UViG)

characteristic: detection type

text: environmental material

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Freshwater lake

text: Freshwater lake

characteristic: local environmental context

text: freshwater metagenome

characteristic: metagenomic source

text: uncultured Caudovirales phage

text: undetermined

characteristic: predicted genome structure

text: dsDNA

characteristic: predicted genome type

characteristic: project name

characteristic: sample derived from

text: uncultured Caudovirales phage

text: metagenome (not viral targeted)

text: VIBRANT; default settings


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: UViG assembly quality

text: MEGAHIT; kmer set 49,69,89,109,129,219

text: Freshwater lake

characteristic: description

text: independent sequence (UViG)

text: environmental material

text: Czech Republic

text: Freshwater lake

text: Freshwater lake

text: freshwater metagenome

text: uncultured Caudovirales phage

text: undetermined

text: dsDNA

text: uncultured Caudovirales phage

text: metagenome (not viral targeted)

characteristic: source of UViGs

text: VIBRANT; default settings

characteristic: viral identification software


text: public

text: MEGAHIT; kmer set 49,69,89,109,129,220

characteristic: assembly software

text: Freshwater lake

characteristic: broad-scale environmental context

characteristic: collection date

text: independent sequence (UViG)

characteristic: detection type

text: environmental material

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Freshwater lake

text: Freshwater lake

characteristic: local environmental context

text: freshwater metagenome

characteristic: metagenomic source

text: uncultured Caudovirales phage

characteristic: organism

text: undetermined

characteristic: predicted genome structure

text: dsDNA

characteristic: predicted genome type

text: uncultured Caudovirales phage

text: metagenome (not viral targeted)

characteristic: title

text: VIBRANT; default settings


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: UViG assembly quality

text: MEGAHIT; kmer set 49,69,89,109,129,229

text: Freshwater lake

characteristic: description

text: independent sequence (UViG)

text: environmental material

text: Czech Republic

text: Freshwater lake

text: Freshwater lake

text: freshwater metagenome

text: uncultured Caudovirales phage

text: undetermined

text: dsDNA

characteristic: project name

characteristic: sample derived from

text: uncultured Caudovirales phage

text: metagenome (not viral targeted)

text: VIBRANT; default settings


text: public

text: MEGAHIT; kmer set 49,69,89,109,129,231

characteristic: assembly software

text: Freshwater lake

characteristic: broad-scale environmental context

characteristic: collection date

text: independent sequence (UViG)

characteristic: detection type

text: environmental material

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Freshwater lake

text: Freshwater lake

characteristic: local environmental context

text: freshwater metagenome

characteristic: metagenomic source

text: uncultured Caudovirales phage

characteristic: organism

text: undetermined

characteristic: predicted genome structure

text: dsDNA

text: uncultured Caudovirales phage

text: metagenome (not viral targeted)

characteristic: source of UViGs

text: VIBRANT; default settings

characteristic: viral identification software


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: UViG assembly quality

text: MEGAHIT; kmer set 49,69,89,109,129,243

text: Freshwater lake

characteristic: description

text: independent sequence (UViG)

text: environmental material

text: Czech Republic

text: Freshwater lake

text: Freshwater lake

text: freshwater metagenome

text: uncultured Caudovirales phage

text: undetermined

text: dsDNA

characteristic: predicted genome type

text: uncultured Caudovirales phage

text: metagenome (not viral targeted)

characteristic: title

text: VIBRANT; default settings


text: public

text: MEGAHIT; kmer set 49,69,89,109,129,256

characteristic: assembly software

text: Freshwater lake

characteristic: broad-scale environmental context

characteristic: collection date

text: independent sequence (UViG)

characteristic: detection type

text: environmental material

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Freshwater lake

text: Freshwater lake

characteristic: local environmental context

text: freshwater metagenome

characteristic: metagenomic source

text: uncultured Caudovirales phage

characteristic: organism

text: undetermined

characteristic: predicted genome structure

text: dsDNA

characteristic: project name

characteristic: sample derived from

text: uncultured Caudovirales phage

text: metagenome (not viral targeted)

text: VIBRANT; default settings


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: UViG assembly quality

text: MEGAHIT; kmer set 49,69,89,109,129,391

text: Freshwater lake

characteristic: description

text: independent sequence (UViG)

text: environmental material

text: Czech Republic

text: Freshwater lake

text: Freshwater lake

text: freshwater metagenome

text: uncultured Caudovirales phage

text: undetermined

text: dsDNA

text: uncultured Caudovirales phage

text: metagenome (not viral targeted)

characteristic: source of UViGs

text: VIBRANT; default settings

characteristic: viral identification software


text: public

text: MEGAHIT; kmer set 49,69,89,109,129,415

characteristic: assembly software

text: Freshwater lake

characteristic: broad-scale environmental context

characteristic: collection date

text: independent sequence (UViG)

characteristic: detection type

text: environmental material

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Freshwater lake

text: Freshwater lake

characteristic: local environmental context

text: freshwater metagenome

characteristic: metagenomic source

text: uncultured Caudovirales phage

characteristic: organism

text: undetermined

characteristic: predicted genome structure

text: dsDNA

characteristic: predicted genome type

text: uncultured Caudovirales phage

text: metagenome (not viral targeted)

characteristic: title

text: VIBRANT; default settings


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: UViG assembly quality

text: MEGAHIT; kmer set 49,69,89,109,129,417

text: Freshwater lake

characteristic: description

text: independent sequence (UViG)

text: environmental material

text: Czech Republic

text: Freshwater lake

text: Freshwater lake

text: freshwater metagenome

text: uncultured Caudovirales phage

text: undetermined

text: dsDNA

characteristic: project name

characteristic: sample derived from

text: uncultured Caudovirales phage

text: metagenome (not viral targeted)

text: VIBRANT; default settings


text: public

text: MEGAHIT; kmer set 49,69,89,109,129,462

characteristic: assembly software

text: Freshwater lake

characteristic: broad-scale environmental context

characteristic: collection date

text: independent sequence (UViG)

characteristic: detection type

text: environmental material

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Freshwater lake

text: Freshwater lake

characteristic: local environmental context

text: freshwater metagenome

characteristic: metagenomic source

text: uncultured Caudovirales phage

characteristic: organism

text: undetermined

characteristic: predicted genome structure

text: dsDNA

text: uncultured Caudovirales phage

text: metagenome (not viral targeted)

characteristic: source of UViGs

text: VIBRANT; default settings

characteristic: viral identification software


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: UViG assembly quality

text: MEGAHIT; kmer set 49,69,89,109,129,563

text: Freshwater lake

characteristic: description

text: independent sequence (UViG)

text: environmental material

text: Czech Republic

text: Freshwater lake

text: Freshwater lake

text: freshwater metagenome

text: uncultured Caudovirales phage

text: undetermined

text: dsDNA

characteristic: predicted genome type

text: uncultured Caudovirales phage

text: metagenome (not viral targeted)

characteristic: title

text: VIBRANT; default settings


text: public

text: MEGAHIT; kmer set 49,69,89,109,129,656

characteristic: assembly software

text: Freshwater lake

characteristic: broad-scale environmental context

characteristic: collection date

text: independent sequence (UViG)

characteristic: detection type

text: environmental material

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Freshwater lake

text: Freshwater lake

characteristic: local environmental context

text: freshwater metagenome

characteristic: metagenomic source

text: uncultured Caudovirales phage

characteristic: organism

text: undetermined

characteristic: predicted genome structure

text: dsDNA

characteristic: project name

characteristic: sample derived from

text: uncultured Caudovirales phage

text: metagenome (not viral targeted)

text: VIBRANT; default settings


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: UViG assembly quality

text: MEGAHIT; kmer set 49,69,89,109,129,657

text: Freshwater lake

characteristic: description

text: independent sequence (UViG)

text: environmental material

text: Czech Republic

text: Freshwater lake

text: Freshwater lake

text: freshwater metagenome

text: uncultured Caudovirales phage

text: undetermined

text: dsDNA

text: uncultured Caudovirales phage

text: metagenome (not viral targeted)

characteristic: source of UViGs

text: VIBRANT; default settings

characteristic: viral identification software


text: public

text: MEGAHIT; kmer set 49,69,89,109,129,658

characteristic: assembly software

text: Freshwater lake

characteristic: broad-scale environmental context

characteristic: collection date

text: independent sequence (UViG)

characteristic: detection type

text: environmental material

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Freshwater lake

text: Freshwater lake

characteristic: local environmental context

text: freshwater metagenome

characteristic: metagenomic source

text: uncultured Caudovirales phage

characteristic: organism

text: undetermined

characteristic: predicted genome structure

text: dsDNA

characteristic: predicted genome type

text: uncultured Caudovirales phage

text: metagenome (not viral targeted)

characteristic: title

text: VIBRANT; default settings


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: UViG assembly quality

text: MEGAHIT; kmer set 49,69,89,109,129,659

text: Freshwater lake

characteristic: description

text: independent sequence (UViG)

text: environmental material

text: Czech Republic

text: Freshwater lake

text: Freshwater lake

text: freshwater metagenome

text: uncultured Caudovirales phage

text: undetermined

text: dsDNA

characteristic: project name

characteristic: sample derived from

text: uncultured Caudovirales phage

text: metagenome (not viral targeted)

text: VIBRANT; default settings


text: public

text: MEGAHIT; kmer set 49,69,89,109,129,660

characteristic: assembly software

text: Freshwater lake

characteristic: broad-scale environmental context

characteristic: collection date

text: independent sequence (UViG)

characteristic: detection type

text: environmental material

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Freshwater lake

text: Freshwater lake

characteristic: local environmental context

text: freshwater metagenome

characteristic: metagenomic source

text: uncultured Caudovirales phage

characteristic: organism

text: undetermined

characteristic: predicted genome structure

text: dsDNA

text: uncultured Caudovirales phage

text: metagenome (not viral targeted)

characteristic: source of UViGs

text: VIBRANT; default settings

characteristic: viral identification software


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

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characteristic: UViG assembly quality

text: MEGAHIT; kmer set 49,69,89,109,129,661

text: Freshwater lake

characteristic: description

text: independent sequence (UViG)

text: environmental material

text: Czech Republic

text: Freshwater lake

text: Freshwater lake

text: freshwater metagenome

text: uncultured Caudovirales phage

text: undetermined

text: dsDNA

characteristic: predicted genome type

text: uncultured Caudovirales phage

text: metagenome (not viral targeted)

characteristic: title

text: VIBRANT; default settings


text: public

text: MEGAHIT; kmer set 49,69,89,109,129,662

characteristic: assembly software

text: Freshwater lake

characteristic: broad-scale environmental context

characteristic: collection date

text: independent sequence (UViG)

characteristic: detection type

text: environmental material

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Freshwater lake

text: Freshwater lake

characteristic: local environmental context

text: freshwater metagenome

characteristic: metagenomic source

text: uncultured Caudovirales phage

characteristic: organism

text: undetermined

characteristic: predicted genome structure

text: dsDNA

characteristic: project name

characteristic: sample derived from

text: uncultured Caudovirales phage

text: metagenome (not viral targeted)

text: VIBRANT; default settings


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: UViG assembly quality

text: MEGAHIT; kmer set 49,69,89,109,129,663

text: Freshwater lake

characteristic: description

text: independent sequence (UViG)

text: environmental material

text: Czech Republic

text: Freshwater lake

text: Freshwater lake

text: freshwater metagenome

text: uncultured Caudovirales phage

text: undetermined

text: dsDNA

text: uncultured Caudovirales phage

text: metagenome (not viral targeted)

characteristic: source of UViGs

text: VIBRANT; default settings

characteristic: viral identification software


text: public

text: MEGAHIT; kmer set 49,69,89,109,129,664

characteristic: assembly software

text: Freshwater lake

characteristic: broad-scale environmental context

characteristic: collection date

text: independent sequence (UViG)

characteristic: detection type

text: environmental material

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Freshwater lake

text: Freshwater lake

characteristic: local environmental context

text: freshwater metagenome

characteristic: metagenomic source

text: uncultured Caudovirales phage

characteristic: organism

text: undetermined

characteristic: predicted genome structure

text: dsDNA

characteristic: predicted genome type

text: uncultured Caudovirales phage

text: metagenome (not viral targeted)

characteristic: title

text: VIBRANT; default settings


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: UViG assembly quality

text: MEGAHIT

text: Freshwater lake

characteristic: description

text: independent sequence (UViG)

text: environmental material

text: Czech Republic

text: Freshwater lake

text: Freshwater lake

text: freshwater metagenome

text: uncultured Caudovirales phage

text: undetermined

text: dsDNA

characteristic: project name

characteristic: sample derived from

text: uncultured Caudovirales phage

text: metagenome (not viral targeted)

text: VIBRANT; default settings


text: public

text: MEGAHIT; kmer set 49,69,89,109,129,699

characteristic: assembly software

text: Freshwater lake

characteristic: broad-scale environmental context

characteristic: collection date

text: independent sequence (UViG)

characteristic: detection type

text: environmental material

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Freshwater lake

text: Freshwater lake

characteristic: local environmental context

text: freshwater metagenome

characteristic: metagenomic source

text: uncultured Caudovirales phage

characteristic: organism

text: undetermined

characteristic: predicted genome structure

text: dsDNA

text: uncultured Caudovirales phage

text: metagenome (not viral targeted)

characteristic: source of UViGs

text: VIBRANT; default settings

characteristic: viral identification software


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF OULU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:UNIVERSITY OF OULU

characteristic: Submitter Id

text: cortical astrocyte

text: human

characteristic: common name

text: human adult astrocyte grown in full medium 24hours

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: astrocyte control 1 mRNA

characteristic: title


tag: attribute

text: UNIVERSITY OF OULU

text: public

text: cortical astrocyte

text: human

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: astrocyte control 2 mRNA


text: UNIVERSITY OF OULU

text: public

text: cortical astrocyte

text: human

text: Homo sapiens

text: Homo sapiens

text: astrocyte control 3 mRNA


text: UNIVERSITY OF OULU

text: public

text: cortical astrocyte

text: human

text: human adult astrocyte without FBS 24hours

text: Homo sapiens

text: Homo sapiens

text: astrocyte without FBS 1 mRNA


text: UNIVERSITY OF OULU

text: public

text: cortical astrocyte

text: human

text: human adult astrocytes without FBS 24 hours

text: Homo sapiens

text: Homo sapiens

text: astrocyte without FBS 2 mRNA


text: UNIVERSITY OF OULU

text: public

text: cortical astrocyte

text: human

text: Homo sapiens

text: Homo sapiens

text: astrocyte without FBS 3 mRNA


text: UNIVERSITY OF OULU

text: public

text: cortical astrocyte

text: human

text: adult human astrocyte without FBS from which EVs are collected

text: Homo sapiens

text: Homo sapiens

text: astrocyte cells Qiagen miRNA-seq 1


text: UNIVERSITY OF OULU

text: public

text: cortical astrocyte

text: human

text: Homo sapiens

text: Homo sapiens

text: astrocyte cells Qiagen miRNA-seq 2


text: UNIVERSITY OF OULU

text: public

text: cortical astrocyte

text: human

text: Homo sapiens

text: Homo sapiens

text: astrocyte cells Qiagen miRNA-seq 3


text: UNIVERSITY OF OULU

text: public

text: extracellular vesicles

text: human

text: EVs from human adult astrocytes

text: Homo sapiens

text: Homo sapiens

text: EV medium without FBS miRNA-seq1


text: UNIVERSITY OF OULU

text: public

text: extracellular vesicles

text: human

text: EVs from human adult astrocytes

text: Homo sapiens

text: Homo sapiens

text: EV medium without FBS miRNA-seq2


text: UNIVERSITY OF OULU

text: public

text: extracellular vesicles

text: human

text: EVs from human adult astrocytes

text: Homo sapiens

text: Homo sapiens

text: EV medium without FBS miRNA-seq3


text: UNIVERSITY OF OULU

text: public

text: extracellular vesicles

text: human

text: EVs Proteinase K /RNaseA/RNaseT1 treated

text: Homo sapiens

text: Homo sapiens

text: EVs PKRNase miRNa-seq1


text: UNIVERSITY OF OULU

text: public

text: extracellular vesicles

text: human

text: Homo sapiens

text: Homo sapiens

text: EVs PKRNase miRNa-seq2


text: UNIVERSITY OF OULU

text: public

text: extracellular vesicles

text: human

text: Homo sapiens

text: Homo sapiens

text: EVsPKRNase miRNA-seq3