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text: Skagarak, Koster archipeloago, West Lindholmen

text: Littorina saxatilis

text: Littorina saxatilis

text: F


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, Killingholmen

text: Littorina saxatilis

text: Littorina saxatilis

text: M


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, Lidlerran

text: Littorina saxatilis

text: Littorina saxatilis

text: M


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, Southwest Hamneholmen

text: Littorina saxatilis

text: Littorina saxatilis

text: F


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago,North Hamneholmen

text: Littorina saxatilis

text: Littorina saxatilis

text: F


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, Southwest point of Nordkoster

text: Littorina saxatilis

text: Littorina saxatilis

text: F


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, Gula Sk�ret

text: Littorina saxatilis

text: Littorina saxatilis

text: F


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, H�llsholmen

text: Littorina saxatilis

text: Littorina saxatilis

text: F


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, �lsk�r

text: Littorina saxatilis

text: Littorina saxatilis

text: M


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, Brevik

text: Littorina saxatilis

text: Littorina saxatilis

text: F


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, Tenesk�r

text: Littorina saxatilis

text: Littorina saxatilis

text: F


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, Filjholmen

text: Littorina saxatilis

text: Littorina saxatilis

text: F


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, West Stora Brattsk�r

text: Littorina saxatilis

text: Littorina saxatilis

text: M


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, Northwest Brattsk�r

text: Littorina saxatilis

text: Littorina saxatilis

text: F


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, Lilla Brattsk�r

text: Littorina saxatilis

text: Littorina saxatilis

text: F


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, North Vedsk�r

text: Littorina saxatilis

text: Littorina saxatilis

text: M


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, Southwest Vedsk�r

text: Littorina saxatilis

text: Littorina saxatilis

text: M


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, South T�rnholmen

text: Littorina saxatilis

text: Littorina saxatilis

text: F


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, West Timmerglaset

text: Littorina saxatilis

text: Littorina saxatilis

text: M


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, L�ddersk�ren

text: Littorina saxatilis

text: Littorina saxatilis

text: F


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, T�ngudden

text: Littorina saxatilis

text: Littorina saxatilis

text: F


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, Matkullen

text: Littorina saxatilis

text: Littorina saxatilis

text: F


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, Kl�vsk�r Styrs�

text: Littorina saxatilis

text: Littorina saxatilis

text: M


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, L�ngholmen

text: Littorina saxatilis

text: Littorina saxatilis

text: F


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, Elingholmen

text: Littorina saxatilis

text: Littorina saxatilis

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text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, �lekr�kesk�r

text: Littorina saxatilis

text: Littorina saxatilis

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text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, M�sk�r

text: Littorina saxatilis

text: Littorina saxatilis

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text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, Sk�r near Jutholmen

text: Littorina saxatilis

text: Littorina saxatilis

text: F


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, Nypholmen

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text: University of Gothenburg

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text: University of Gothenburg

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text: Skagarak, Koster archipeloago, East Skarves�tet

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text: public

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text: public

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text: public

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text: Skagarak, Koster archipeloago, Lilla Sockna

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text: public

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text: public

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text: Littorina saxatilis

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text: public

text: Sweden

text: Skagarak, Koster archipeloago, Kyrkosund

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text: Littorina saxatilis

text: F


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, West Kyrkosund

text: Littorina saxatilis

text: Littorina saxatilis

text: F


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, Flate-Guri

text: Littorina saxatilis

text: Littorina saxatilis

text: M


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, Saltholmen

text: Littorina saxatilis

text: Littorina saxatilis

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text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, Hasslevikarna

text: Littorina saxatilis

text: Littorina saxatilis

text: F


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, M�rebukterna

text: Littorina saxatilis

text: Littorina saxatilis

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text: University of Gothenburg

text: public

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text: Skagarak, Koster archipeloago, Eken�s

text: Littorina saxatilis

text: Littorina saxatilis

text: J


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, Kostersundet

text: Littorina saxatilis

text: Littorina saxatilis

text: M


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, Brevik harbour

text: Littorina saxatilis

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text: University of Gothenburg

text: public

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text: Skagarak, Koster archipeloago, R�nnevik

text: Littorina saxatilis

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text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, Skabot�ngen

text: Littorina saxatilis

text: Littorina saxatilis

text: M


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, Lindholmen

text: Littorina saxatilis

text: Littorina saxatilis

text: F


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, Yttre Burholmen

text: Littorina saxatilis

text: Littorina saxatilis

text: F


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, Innre Burholmen

text: Littorina saxatilis

text: Littorina saxatilis

text: M


text: University of Gothenburg

text: public

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text: Skagarak, Koster archipeloago, East Tj�rn�

text: Littorina saxatilis

text: Littorina saxatilis

text: M


text: University of Gothenburg

text: public

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text: Skagarak, Koster archipeloago, Ramnekroken

text: Littorina saxatilis

text: Littorina saxatilis

text: F


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, North Nord �dd�

text: Littorina saxatilis

text: Littorina saxatilis

text: F


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, L�ngestrand

text: Littorina saxatilis

text: Littorina saxatilis

text: M


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, Liggstill

text: Littorina saxatilis

text: Littorina saxatilis

text: F


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, West Bay of Styrs�

text: Littorina saxatilis

text: Littorina saxatilis

text: M


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, Southwest Styrs�

text: Littorina saxatilis

text: Littorina saxatilis

text: M


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, Northwest L�ngholmen

text: Littorina saxatilis

text: Littorina saxatilis

text: F


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, Salt�-Lyngnholmen

text: Littorina saxatilis

text: Littorina saxatilis

text: F


text: University of Gothenburg

text: public

text: Sweden

text: Skagarak, Koster archipeloago, R�vlingsundet

text: Littorina saxatilis

text: Littorina saxatilis

text: F


text: liver

characteristic: ArrayExpress-PHENOTYPE

text: male

characteristic: ArrayExpress-SEX

text: Nothobranchius furzeri

characteristic: ArrayExpress-SPECIES

text: Male

characteristic: GENDER

text: SC

text: public

text: liver

characteristic: PHENOTYPE

text: turquoise killifish

characteristic: common name

text: Nothobranchius furzeri

text: Nothobranchius furzeri


text: gut

text: male

text: Nothobranchius furzeri

characteristic: ENA-CHECKLIST

characteristic: External Id

text: Male

text: SC

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: gut

characteristic: SRA accession

text: turquoise killifish

text: Nothobranchius furzeri

text: Nothobranchius furzeri


text: muscle

text: male

text: Nothobranchius furzeri

characteristic: ENA-FIRST-PUBLIC

text: Male

text: SC

text: public

text: muscle

characteristic: Submitter Id

text: turquoise killifish

text: Nothobranchius furzeri

characteristic: organism

text: Nothobranchius furzeri

characteristic: title


text: unidentified

characteristic: ENA-LAST-UPDATE

text: SC

characteristic: INSDC first public

text: public

text: unidentified

text: unidentified

text: unidentified


text: public

tag: Namespace:Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry

unit: year

characteristic: age

text: ArrayExpress

characteristic: broker name

text: CD4-positive, alpha-beta T cell

text: human

text: Protocols: Venous blood or synovial fluid was collected in EDTA Vacutainers (BD). For three patients samples were collected at two time points 9-23 months apart (two peaks of synovitis, with some remission in between). The rest of the samples were collected once during a synovitis episode. The samples were diluted three times in PBS and layered onto Ficoll (PanEco, Russia), then centrifuged at 300 g for 40 minutes at room temperature and washed 2 times with PBS. At least 3x10^6 of obtained MNCs were freshly lysed for total T-cell samples or used to isolate CD+ and CD8+ T-cell subsets. CD4+ T-cells were isolated from MNCs using anti-CD4 magnetic beads (Dynabeads, ThermoFisher), and then supernatant containing unbound cells was used to isolate CD8+ T-cells using anti-CD8 magnetic beads (Dynabeads, ThermoFisher). The obtained cell subsets were lysed with TRIzol reagent (Invitrogen). Total RNA was extracted with Trizol following manufacturer protocol Libraries of TCRbeta chains were prepared as previously described (Zvyagin et al., Leukemia, 2017). In brief, total RNA was used for cDNA synthesis with 5’RACE template switch technology to introduce universal primer binding site, first sample barcode and Unique Molecular Identifiers (UMI) at the 5’ end of RNA molecules. Primers complementary to TCRbeta constant segments were used for cDNA synthesis initiation. cDNA was amplified in two subsequent PCR steps. During the second PCR step, second sample barcode and sequence adapters were introduced to the libraries.

characteristic: description

text: adult

characteristic: developmental stage

text: psoriatic arthritis

characteristic: disease

text: Ali

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

characteristic: organism part

characteristic: replicate

text: first synovitis episode

characteristic: sampling time point

text: Homo sapiens

text: male

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characteristic: External Id

text: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry

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text: ArrayExpress

text: CD8-positive, alpha-beta T cell

text: human

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text: adult

text: psoriatic arthritis

text: Ali

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: first synovitis episode

text: Homo sapiens

text: male


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characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: T cell

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characteristic: organism

text: Homo sapiens

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text: male

characteristic: title


text: public

text: ArrayExpress

characteristic: broker name

text: CD4-positive, alpha-beta T cell

text: human

characteristic: description

text: adult

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text: psoriatic arthritis

characteristic: disease

text: Ali

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text: Homo sapiens

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characteristic: replicate

text: first synovitis episode

text: Homo sapiens

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text: public

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text: ArrayExpress

text: CD8-positive, alpha-beta T cell

text: human

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text: Ali

text: Homo sapiens

text: Homo sapiens

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text: first synovitis episode

characteristic: sampling time point

text: Homo sapiens

text: male

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text: ArrayExpress

text: T cell

text: human

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text: adult

text: ankylosing spondylitis

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text: Homo sapiens

text: Homo sapiens

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text: first synovitis episode

text: Homo sapiens

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text: ArrayExpress

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text: CD4-positive, alpha-beta T cell

text: human

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text: Homo sapiens

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text: Homo sapiens

text: synovial fluid

text: first synovitis episode

text: Homo sapiens

text: male

characteristic: title


characteristic: ENA-CHECKLIST

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text: ArrayExpress

text: CD8-positive, alpha-beta T cell

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text: adult

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characteristic: organism part

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text: Homo sapiens

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text: CD4-positive, alpha-beta T cell

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text: ArrayExpress

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text: Homo sapiens

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text: female

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characteristic: ENA-FIRST-PUBLIC

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text: ArrayExpress

text: CD4-positive, alpha-beta T cell

text: human

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text: psoriatic arthritis

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text: CD8-positive, alpha-beta T cell

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text: Homo sapiens

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characteristic: ENA-CHECKLIST

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text: public

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text: ArrayExpress

text: T cell

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characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: CD4-positive, alpha-beta T cell

text: human

text: adult

text: psoriatic arthritis

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text: Homo sapiens

characteristic: organism

text: Homo sapiens

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text: ArrayExpress

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text: Homo sapiens

text: Homo sapiens

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text: Homo sapiens

text: Homo sapiens

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text: Homo sapiens

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text: first synovitis episode

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text: Homo sapiens

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text: male

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characteristic: ENA-CHECKLIST

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text: public

text: ArrayExpress

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characteristic: organism

text: Homo sapiens

text: peripheral blood

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text: female

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text: ArrayExpress

characteristic: broker name

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text: human

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text: psoriatic arthritis

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text: Homo sapiens

text: Homo sapiens

text: synovial fluid

text: first synovitis episode

text: Homo sapiens

text: female


characteristic: ENA-FIRST-PUBLIC

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text: ArrayExpress

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text: first synovitis episode

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text: Homo sapiens

text: male

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characteristic: INSDC last update

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characteristic: SRA accession

characteristic: Submitter Id

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text: human

text: adult

text: psoriatic arthritis

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characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: first synovitis episode

text: Homo sapiens

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characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

text: ArrayExpress

characteristic: broker name

text: CD8-positive, alpha-beta T cell

text: human

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text: Homo sapiens

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text: Homo sapiens

text: synovial fluid

text: first synovitis episode

text: Homo sapiens

text: male

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characteristic: ENA-FIRST-PUBLIC

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characteristic: INSDC center name

text: public

text: ArrayExpress

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text: adult

text: psoriatic arthritis

text: Uma

text: Homo sapiens

text: Homo sapiens

text: synovial fluid

characteristic: organism part

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text: first synovitis episode

text: Homo sapiens

text: male


characteristic: INSDC first public

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text: public

characteristic: INSDC status

characteristic: age

text: ArrayExpress

text: CD4-positive, alpha-beta T cell

text: human

text: adult

characteristic: developmental stage

text: psoriatic arthritis

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text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: first synovitis episode

characteristic: sampling time point

text: Homo sapiens

text: male

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: CD8-positive, alpha-beta T cell

text: human

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text: psoriatic arthritis

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text: first synovitis episode

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text: male


characteristic: ENA-FIRST-PUBLIC

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text: Homo sapiens

characteristic: organism

text: Homo sapiens

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text: male

characteristic: title


characteristic: INSDC first public

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text: public

characteristic: INSDC status

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text: ArrayExpress

text: CD4-positive, alpha-beta T cell

text: human

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text: Homo sapiens

text: synovial fluid

characteristic: organism part

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characteristic: ENA-CHECKLIST

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text: public

characteristic: SRA accession

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text: ArrayExpress

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text: CD8-positive, alpha-beta T cell

text: human

text: adult

text: psoriatic arthritis

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text: Homo sapiens

text: Homo sapiens

text: synovial fluid

text: first synovitis episode

characteristic: sampling time point

text: Homo sapiens

text: male

characteristic: sex


characteristic: ENA-FIRST-PUBLIC

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text: public

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text: T cell

text: human

characteristic: common name

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text: ankylosing spondylitis

characteristic: disease

text: Zar

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: first synovitis episode

text: Homo sapiens

text: male


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

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text: ArrayExpress

text: CD4-positive, alpha-beta T cell

text: human

text: adult

characteristic: developmental stage

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text: Zar

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: synovial fluid

text: first synovitis episode

text: Homo sapiens

text: male

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: CD8-positive, alpha-beta T cell

text: human

text: adult

text: ankylosing spondylitis

text: Zar

text: Homo sapiens

text: Homo sapiens

text: synovial fluid

characteristic: organism part

characteristic: replicate

text: first synovitis episode

text: Homo sapiens

text: male


characteristic: ENA-FIRST-PUBLIC

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characteristic: INSDC center name

text: public

text: ArrayExpress

text: T cell

text: human

characteristic: common name

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text: Zar

text: Homo sapiens

text: Homo sapiens

text: synovial fluid

text: first synovitis episode

characteristic: sampling time point

text: Homo sapiens

text: male

characteristic: sex


characteristic: INSDC first public

characteristic: INSDC last update

text: public

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text: ArrayExpress

text: CD4-positive, alpha-beta T cell

text: human

text: adult

characteristic: developmental stage

text: psoriatic arthritis

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text: Zlt

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text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: first synovitis episode

text: Homo sapiens

text: female


characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

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text: CD8-positive, alpha-beta T cell

text: human

text: adult

text: psoriatic arthritis

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text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: peripheral blood

text: first synovitis episode

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text: female

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characteristic: ENA-FIRST-PUBLIC

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text: public

text: ArrayExpress

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text: human

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text: Homo sapiens

text: Homo sapiens

text: peripheral blood

characteristic: organism part

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text: first synovitis episode

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text: female


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: age

text: ArrayExpress

text: CD4-positive, alpha-beta T cell

text: human

text: adult

characteristic: developmental stage

text: psoriatic arthritis

text: Zlt

text: Homo sapiens

text: Homo sapiens

text: synovial fluid

text: first synovitis episode

characteristic: sampling time point

text: Homo sapiens

text: female

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: CD8-positive, alpha-beta T cell

text: human

text: adult

text: psoriatic arthritis

characteristic: disease

text: Zlt

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: synovial fluid

text: first synovitis episode

text: Homo sapiens

text: female


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC center name

text: public

text: ArrayExpress

text: T cell

text: human

characteristic: common name

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text: adult

text: psoriatic arthritis

text: Zlt

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: synovial fluid

text: first synovitis episode

text: Homo sapiens

text: female

characteristic: title


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: age

text: ArrayExpress

text: T cell

text: human

text: adult

characteristic: developmental stage

text: psoriatic arthritis

text: Zlt

text: Homo sapiens

text: Homo sapiens

text: synovial fluid

characteristic: organism part

characteristic: replicate

text: second synovitis episode

text: Homo sapiens

text: female


characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

characteristic: SRA accession

tag: Namespace:Bioinformatics and Omics Data Science Group, BIMSB, Berlin

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: epithelial cell

text: human

text: Protocols: Cells were resuspended in 50 mL PBS/108 cells and fixed by adding 1% formaldehyde for 10 min. Formaldehyde was quenched by addition of 0.6 M glycine pH 3 for 15 min. Cells were washed twice in cold PBS. Nuclei were isolated by resuspending the cells in 5 mL cold lysis buffer 1 (50 mM HEPES/KOH pH 7.5, 140 mM NaCl, 1 mM EDTA, 10% glycerol, 0.5% Nonidet P-40, 0.25% Triton X-100, 1x Complete protease inhibitors (Roche)) per 108 cells and rotating at 4°C for 10 min. After centrifugation nuclei were resuspended in 5mL/108 cells cold lysis buffer 2 (10 mM Tris-Cl pH 8, 200 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 1x Complete protease inhibitors (Roche)) and rotated for 10 min at room temperature. The pellet after centrifugation was resuspended in 3 mL lysis buffer 3 (10 mM Tris-Cl pH 8, 100 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 0.1% Na-deoxycholate, 0.5% N-lauroylsarcosine, 1x Complete protease inhibitors (Roche)) and chromatin was sheared to an average size of 200-600 bp by sonication for 20 cycles 30 sec on/30 sec off using Bioruptor Pico (Diagenode). 1% Triton X-100 was added after sonication. 5-10% of chromatin was kept as an input control for qPCR. For ChIP-seq 1.5% spike-in chromatin from a mouse cell line expressing endogenously tagged PHF3-GFP was added. Anti-GFP antiserum (Abcam ab290) was added to sheared chromatin and rotated ON at 4°C. Protein A Dynabeads (Invitrogen) were washed in cold block solution (0.5% BSA in PBS) three times, mixed with antibody-bound chromatin and rotated for 4-6 h at 4°C. Beads were washed in RIPA washing buffer (50 mM Hepes/KOH pH 7.5, 500 mM LiCl, 1 mM EDTA, 1% NP-40, 0.7% Na-deoxycholate) five times and in 50 mM NaCl in TE once. Immunoprecipitated protein-DNA complexes were eluted in 200 µL elution buffer (50 mM Tris-Cl pH 8, 10 mM EDTA, 1% SDS) for 15 min at 65°C. Eluates were incubated ON at 65°C to reverse crosslinks, treated with 0.2 mg/mL RNase A for 2 h at 37°C and 0.2 mg/mL proteinase K and 5.25 mM CaCl2 for 30 min at 55°C. DNA was purified by phenol-chloroform extraction followed by ethanol precipitation and resuspended in 50 µL nuclease free water. ChIP-seq libraries were prepared using NEBNext Ultra II DNA library prep kit for Illumina (New England Biolabs) and NEBNext Multiplex Oligos for Illumina (New England Biolabs) according to the manufacturer’s instructions.

text: embryo

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: kidney

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Bioinformatics and Omics Data Science Group, BIMSB, Berlin

characteristic: INSDC center name

text: public

text: ArrayExpress

text: epithelial cell

text: human

characteristic: common name

text: embryo

text: Homo sapiens

text: Homo sapiens

text: kidney

text: Homo sapiens


text: mixed

characteristic: ArrayExpress-SEX

text: Oscheius sp. AF13

characteristic: ArrayExpress-SPECIES

text: Mixed

characteristic: GENDER

text: SC

characteristic: INSDC first public

text: public

text: Oscheius sp. AF13

text: Oscheius sp. AF5

characteristic: organism

text: Oscheius sp. AF5

characteristic: title


text: mixed

text: Oscheius sp. SB394

text: Mixed

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Oscheius sp. SB394

text: Oscheius sp. SB394

text: Oscheius sp. SB394


text: mixed

text: Oscheius dolichuroides

characteristic: ENA-CHECKLIST

characteristic: External Id

text: Mixed

text: SC

text: public

text: Oscheius dolichuroides

text: Oscheius dolichuroides

text: Oscheius dolichuroides


text: mixed

text: Oscheius sp. SB194

text: Mixed

text: SC

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Oscheius sp. SB194

text: Oscheius sp. SB194

text: Oscheius sp. SB194


text: mixed

text: Oscheius sp. JU1182

text: Mixed

text: SC

text: public

text: Oscheius sp. JU1182

text: Oscheius sp. JU1182

text: Oscheius sp. JU1182


text: mixed

text: Oscheius sp. JU1917

text: Mixed

text: SC

text: public

text: Oscheius sp. JU1917

text: Oscheius sp. JU1917

text: Oscheius sp. JU1917


text: mixed

text: Auanema freiburgensis

text: Mixed

text: SC

text: public

text: Auanema freiburgensis

text: Auanema freiburgensis

text: Auanema freiburgensis


text: mixed

text: Auanema sp. APS25

text: Mixed

text: SC

text: public

text: Auanema sp. APS25

text: Auanema sp. APS25

text: Auanema sp. APS25


text: mixed

text: Auanema sp. NKZ329

text: Mixed

text: SC

text: public

text: Auanema sp. NKZ329

text: Auanema sp. NKZ329

text: Auanema sp. NKZ329


text: mixed

text: Oscheius myriophilus

text: Mixed

text: SC

text: public

text: Oscheius myriophilus

text: Oscheius myriophilus

text: Oscheius myriophilus


text: mixed

text: Oscheius chongmingensis

text: Mixed

text: SC

text: public

text: Oscheius chongmingensis

text: Oscheius chongmingensis

text: Oscheius chongmingensis


text: public

tag: Namespace:GFZ German Research Centre for Geosciences

characteristic: collection date

text: 9 m control, 13 degree, 154 days incubation

characteristic: description

text: permafrost

characteristic: environment (biome)

text: permafrost

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

unit: m

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: metagenome

text: Vault permafrost tunnel, Alaska, inoculation study

characteristic: project name

text: metagenome

text: Illumina HiSeq 2x150

characteristic: sequencing method

text: soil

characteristic: soil environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: GFZ German Research Centre for Geosciences

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: 9 m control, 3 degree, 154 days incubation

text: permafrost

text: permafrost

text: soil

text: USA

text: metagenome

text: metagenome

characteristic: organism

text: metagenome

text: Illumina HiSeq 2x150

text: soil

characteristic: title


text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: 9 m control, 0 day incubation

characteristic: description

text: permafrost

characteristic: environment (biome)

text: permafrost

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

text: metagenome

text: metagenome

text: metagenome

text: Illumina HiSeq 2x150

text: soil


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: 9 m inoculated, 13 degree, 154 days incubation

text: permafrost

text: permafrost

text: soil

text: USA

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: metagenome

characteristic: project name

text: metagenome

text: Illumina HiSeq 2x150

characteristic: sequencing method

text: soil

characteristic: soil environmental package


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: 9 m inoculated, 3 degree, 154 days incubation

characteristic: description

text: permafrost

text: permafrost

text: soil

text: USA

text: metagenome

text: metagenome

characteristic: organism

text: metagenome

text: Illumina HiSeq 2x150

text: soil

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: 12 m control, 13 degree, 154 days incubation

text: permafrost

characteristic: environment (biome)

text: permafrost

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

text: metagenome

text: metagenome

text: metagenome

text: Illumina HiSeq 2x150

text: soil