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characteristic: local environmental context

text: uncultured bacterium

text: uncultured bacterium


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Aarhus University

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-09-12-2022-14:29:02:102-75172

characteristic: Submitter Id

text: Woodchip

text: Denmark

text: Denitrifying bioreactor

text: uncultured bacterium

text: uncultured bacterium


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Aarhus University

text: public

text: ena-SAMPLE-TAB-09-12-2022-14:29:02:102-75173

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

characteristic: description

text: Woodchip

text: Denmark

text: Denitrifying bioreactor

text: uncultured bacterium

text: uncultured bacterium


text: Aarhus University

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ena-SAMPLE-TAB-09-12-2022-14:29:02:102-75174

text: Woodchip

characteristic: environmental medium

text: Denmark

text: Denitrifying bioreactor

text: uncultured bacterium

characteristic: organism

characteristic: project name

text: uncultured bacterium

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Aarhus University

text: public

text: ena-SAMPLE-TAB-09-12-2022-14:29:02:102-75175

text: Woodchip

text: Denmark

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Denitrifying bioreactor

characteristic: local environmental context

text: uncultured bacterium

text: uncultured bacterium


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Aarhus University

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-09-12-2022-14:29:02:102-75176

characteristic: Submitter Id

text: Woodchip

text: Denmark

text: Denitrifying bioreactor

text: uncultured bacterium

text: uncultured bacterium


text: Aarhus University

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ena-SAMPLE-TAB-09-12-2022-14:29:02:102-75177

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

characteristic: description

text: Woodchip

text: Denmark

text: Denitrifying bioreactor

text: uncultured bacterium

text: uncultured bacterium


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Aarhus University

text: public

text: ena-SAMPLE-TAB-09-12-2022-14:29:02:102-75178

text: Woodchip

characteristic: environmental medium

text: Denmark

text: Denitrifying bioreactor

text: uncultured bacterium

characteristic: organism

characteristic: project name

text: uncultured bacterium

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Aarhus University

text: public

text: ena-SAMPLE-TAB-09-12-2022-14:29:02:102-75179

text: Woodchip

text: Denmark

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Denitrifying bioreactor

characteristic: local environmental context

text: uncultured bacterium

text: uncultured bacterium


text: Aarhus University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-09-12-2022-14:29:02:102-75180

characteristic: Submitter Id

text: Woodchip

text: Denmark

text: Denitrifying bioreactor

text: uncultured bacterium

text: uncultured bacterium


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Aarhus University

text: public

text: ena-SAMPLE-TAB-09-12-2022-14:29:02:102-75181

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

characteristic: description

text: Woodchip

text: Denmark

text: Denitrifying bioreactor

text: uncultured bacterium

text: uncultured bacterium


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Aarhus University

text: public

text: ena-SAMPLE-TAB-09-12-2022-14:29:02:102-75182

text: Woodchip

characteristic: environmental medium

text: Denmark

text: Denitrifying bioreactor

text: uncultured bacterium

characteristic: organism

characteristic: project name

text: uncultured bacterium

characteristic: title


text: Aarhus University

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ena-SAMPLE-TAB-09-12-2022-14:29:02:102-75183

text: Woodchip

text: Denmark

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Denitrifying bioreactor

characteristic: local environmental context

text: uncultured bacterium

text: uncultured bacterium


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Aarhus University

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-09-12-2022-14:29:02:102-75184

characteristic: Submitter Id

text: Woodchip

text: Denmark

text: Denitrifying bioreactor

text: uncultured bacterium

text: uncultured bacterium


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Aarhus University

text: public

text: ena-SAMPLE-TAB-09-12-2022-14:29:02:102-75185

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

characteristic: description

text: Woodchip

text: Denmark

text: Denitrifying bioreactor

text: uncultured bacterium

text: uncultured bacterium


text: Aarhus University

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ena-SAMPLE-TAB-09-12-2022-14:29:02:102-75186

text: Woodchip

characteristic: environmental medium

text: Denmark

text: Denitrifying bioreactor

text: uncultured bacterium

characteristic: organism

characteristic: project name

text: uncultured bacterium

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Aarhus University

text: public

text: ena-SAMPLE-TAB-09-12-2022-14:29:02:103-75187

text: Woodchip

text: Denmark

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Denitrifying bioreactor

characteristic: local environmental context

text: uncultured bacterium

text: uncultured bacterium


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Aarhus University

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-09-12-2022-14:29:02:103-75188

characteristic: Submitter Id

text: Woodchip

text: Denmark

text: Denitrifying bioreactor

text: uncultured bacterium

text: uncultured bacterium


text: Aarhus University

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ena-SAMPLE-TAB-09-12-2022-14:29:02:103-75189

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

characteristic: description

text: Woodchip

text: Denmark

text: Denitrifying bioreactor

text: uncultured bacterium

text: uncultured bacterium


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Aarhus University

text: public

text: ena-SAMPLE-TAB-09-12-2022-14:29:02:103-75190

text: Woodchip

characteristic: environmental medium

text: Denmark

text: Denitrifying bioreactor

text: uncultured bacterium

characteristic: organism

characteristic: project name

text: uncultured bacterium

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Aarhus University

text: public

text: ena-SAMPLE-TAB-09-12-2022-14:29:02:103-75191

text: Woodchip

text: Denmark

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Denitrifying bioreactor

characteristic: local environmental context

text: uncultured bacterium

text: uncultured bacterium


text: Aarhus University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-09-12-2022-14:29:02:103-75192

characteristic: Submitter Id

text: Woodchip

text: Denmark

text: Denitrifying bioreactor

text: uncultured bacterium

text: uncultured bacterium


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Aarhus University

text: public

text: ena-SAMPLE-TAB-09-12-2022-14:29:02:103-75193

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

characteristic: description

text: Woodchip

text: Denmark

text: Denitrifying bioreactor

text: uncultured bacterium

text: uncultured bacterium


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Aarhus University

text: public

text: ena-SAMPLE-TAB-09-12-2022-14:29:02:103-75194

text: Woodchip

characteristic: environmental medium

text: Denmark

text: Denitrifying bioreactor

text: uncultured bacterium

characteristic: organism

characteristic: project name

text: uncultured bacterium

characteristic: title


text: Aarhus University

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ena-SAMPLE-TAB-09-12-2022-14:29:02:103-75195

text: Woodchip

text: Denmark

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Denitrifying bioreactor

characteristic: local environmental context

text: uncultured bacterium

text: uncultured bacterium


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Aarhus University

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-09-12-2022-14:29:02:103-75196

characteristic: Submitter Id

text: Woodchip

text: Denmark

text: Denitrifying bioreactor

text: uncultured bacterium

text: uncultured bacterium


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Aarhus University

text: public

text: ena-SAMPLE-TAB-09-12-2022-14:29:02:103-75197

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

characteristic: description

text: Woodchip

text: Denmark

text: Denitrifying bioreactor

text: uncultured bacterium

text: uncultured bacterium


text: Aarhus University

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ena-SAMPLE-TAB-09-12-2022-14:29:02:103-75198

text: Woodchip

characteristic: environmental medium

text: Denmark

text: Denitrifying bioreactor

text: uncultured bacterium

characteristic: organism

characteristic: project name

text: uncultured bacterium

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Aarhus University

text: public

text: ena-SAMPLE-TAB-09-12-2022-14:29:02:103-75199

text: Woodchip

text: Denmark

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Denitrifying bioreactor

characteristic: local environmental context

text: uncultured bacterium

text: uncultured bacterium


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Aarhus University

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-09-12-2022-14:29:02:103-75200

characteristic: Submitter Id

text: Woodchip

text: Denmark

text: Denitrifying bioreactor

text: uncultured bacterium

text: uncultured bacterium


characteristic: INSDC center name

characteristic: INSDC first public

text: public

tag: Namespace:BIOLOGY CENTRE ASCR, V.V.I., INSTITUTE OF HYDROBIO

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: MIGAHIT

characteristic: assembly software

text: coverage and kmer

characteristic: binning parameters

characteristic: binning software

text: Brackish shallow lake

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Microcystia aeruginosa strain M10 grown in BG11 medium at 22oC under controlled 16h:8h light:dark conditions (white LED, 25 umol photon m-2 s-1) isolated from Lake Tasaul, Romania

characteristic: description

text: lake water

text: Romania

text: Brackish lake

text: brackish water

text: lake water metagenome

characteristic: metagenomic source

text: Microcystis aeruginosa

characteristic: sample derived from

text: Microcystis aeruginosa

text: multi-marker approach

characteristic: taxonomic identity marker


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BIOLOGY CENTRE ASCR, V.V.I., INSTITUTE OF HYDROBIO

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Multiple fragments where gaps span repetitive regions. Presence of the 23S, 16S, and 5S rRNA genes and at least 18 tRNAs

text: MIGAHIT

text: coverage and kmer

text: Brackish shallow lake

text: CheckM

text: Microcystia aeruginosa strain M10 grown in BG11 medium at 26oC under controlled 16h:8h light:dark conditions (white LED, 25 umol photon m-2 s-1) isolated from Lake Tasaul, Romania

text: lake water

characteristic: environmental medium

text: Romania

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Brackish lake

text: brackish water

characteristic: local environmental context

text: lake water metagenome

text: Microcystis aeruginosa

characteristic: organism

characteristic: project name

text: Microcystis aeruginosa

text: multi-marker approach


characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

characteristic: assembly quality

text: MIGAHIT

characteristic: assembly software

text: coverage and kmer

characteristic: binning parameters

characteristic: binning software

text: Brackish shallow lake

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Microcystia aeruginosa strain M11 grown in BG11 medium at 22oC under controlled 16h:8h light:dark conditions (white LED, 25 umol photon m-2 s-1) isolated from Lake Tasaul, Romania

characteristic: description

text: lake water

text: Romania

text: Brackish lake

text: brackish water

text: lake water metagenome

characteristic: metagenomic source

text: Microcystis aeruginosa

text: Microcystis aeruginosa

text: multi-marker approach


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: MIGAHIT

text: coverage and kmer

text: Brackish shallow lake

text: CheckM

text: Microcystia aeruginosa strain M11 grown in BG11 medium at 26oC under controlled 16h:8h light:dark conditions (white LED, 25 umol photon m-2 s-1) isolated from Lake Tasaul, Romania

text: lake water

characteristic: environmental medium

text: Romania

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Brackish lake

text: brackish water

characteristic: local environmental context

text: lake water metagenome

text: Microcystis aeruginosa

characteristic: sample derived from

text: Microcystis aeruginosa

text: multi-marker approach

characteristic: taxonomic identity marker


characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

characteristic: assembly quality

text: MIGAHIT

characteristic: assembly software

text: coverage and kmer

characteristic: binning parameters

characteristic: binning software

text: Brackish shallow lake

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Microcystia aeruginosa strain M12 grown in BG11 medium at 22oC under controlled 16h:8h light:dark conditions (white LED, 25 umol photon m-2 s-1) isolated from Lake Tasaul, Romania

text: lake water

text: Romania

text: Brackish lake

text: brackish water

text: lake water metagenome

text: Microcystis aeruginosa

characteristic: organism

characteristic: project name

text: Microcystis aeruginosa

text: multi-marker approach


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: MIGAHIT

text: coverage and kmer

text: Brackish shallow lake

text: CheckM

text: Microcystia aeruginosa strain M12 grown in BG11 medium at 26oC under controlled 16h:8h light:dark conditions (white LED, 25 umol photon m-2 s-1) isolated from Lake Tasaul, Romania

characteristic: description

text: lake water

characteristic: environmental medium

text: Romania

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Brackish lake

text: brackish water

characteristic: local environmental context

text: lake water metagenome

characteristic: metagenomic source

text: Microcystis aeruginosa

text: Microcystis aeruginosa

text: multi-marker approach


E-MTAB-11739:Bo 103 Gem Pac

text: Mayo Clinic

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11739:Bo 103 Gem Pac

tag: Namespace:Mayo Clinic

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

text: Protocols: PDX13 was established from surgically resected tumor tissue from an adult pancreatic ductal adenocarcinoma (PDAC) patient at the Ruhr-University Bochum Comprehensive Cancer Center. Informed and written consent was obtained. Approval by the ethics committee of the Ruhr University Bochum was obtained under Permission no. 3534-9, 3841-10, 16-5792. Experiments were performed according to the guidelines of the local Animal Use and Care Committees at the Ruhr University Bochum (8.87-50.10.32.09.018, 84-02.04.2012.A328 and 81-02.04.2017.A423). Tumor tissues was implanted in NMRInu/nu mice (both flanks). Tumors were expanded and re-implanted for at least three generations. Later, 1-2mm of tumor tissue (early passage) were implanted subcutaneously in 5-10-week-old mice (NMRInu/nu). Treatment was started when tumors were approximately 100-200 mm3 in size. Mice were randomized into vehicle (09.% saline) or Gemcitabine/Paclitaxel (100/30 mg/kg). The integrity of RNA was validated by gel electrophoresis and 500ng were used to make the libraries in triplicates for each condition. RNA was extracted using Trizol Libraries for cells were made using the TruSeq RNA Library Prep Kit V2 (Illumina) according to the manufacturer’s instructions.

text: adult

characteristic: developmental stage

text: pancreatic ductal adenocarcinoma

characteristic: disease

text: Bo 103

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: pancreas

characteristic: organism part

text: Homo sapiens

text: Bo 103 Gem Pac

characteristic: title

text: subcutaneous injection in NMRInu/nu mice until neoplasm formation

characteristic: xenograft


E-MTAB-11739:Bo 103 Veh

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Mayo Clinic

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11739:Bo 103 Veh

text: ArrayExpress

text: human

characteristic: description

text: adult

text: pancreatic ductal adenocarcinoma

text: Bo 103

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: pancreas

text: Homo sapiens

text: Bo 103 Veh


text: Cornell University

characteristic: INSDC center name

characteristic: INSDC first public

text: public

tag: Namespace:Cornell University

characteristic: Submitter Id

text: Evan Long

text: Fresh young leaves from Buckler lab clone from PI 112679

text: USA

characteristic: geographic location (country and/or sea)

text: Greenhouse, Cornell Univeristy

characteristic: growth facility

text: GRIN

text: Reutealis trisperma

text: Banucalag-nut

characteristic: organism common name

text: soil

characteristic: plant growth medium

text: Fresh leaves

characteristic: plant structure

text: Buckler lab, Cornell Univeristy

characteristic: sample storage location

text: Reutealis trisperma

text: Clone from Buckler collection. Grow from seed from PI 112679

characteristic: source material description

text: PI 112679

characteristic: source material identifiers

text: PI 112679


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cornell University

text: public

text: Evan Long

text: Fresh young leaves from Buckler lab clone from PI 279720

text: USA

text: Greenhouse, Cornell Univeristy

text: GRIN

text: Mercurialis annua

text: Annual mercury

text: soil

text: Fresh leaves

text: Buckler lab, Cornell Univeristy

text: Mercurialis annua

text: Clone from Buckler collection. Grow from cutting from GRIN PI 279720

text: PI 279720

text: PI 279720

characteristic: title


text: Cornell University

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Molly Rooney

text: Leaf from 1996-3422-1 (USA: MOBOT)

characteristic: description

text: USA

text: Field, MOBOT

text: Hesterburg

text: Cnidoscolus aconitifolius

characteristic: organism

text: Tree spinach

text: soil

text: Fresh leaves

text: MOBOT

text: Cnidoscolus aconitifolius

text: Plant from wild grow at MOBOT


text: Cornell University

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: Evan Long

text: Fresh young leaves from Buckler lab clone E002 (TARS 1881)

text: USA

characteristic: geographic location (country and/or sea)

text: Greenhouse, Cornell Univeristy

characteristic: growth facility

text: GRIN

text: Garcia nutans

text: False tung oil tree

text: soil

text: Fresh leaves

text: Buckler lab, Cornell Univeristy

text: Garcia nutans

text: Clone from Buckler collection. Grow from cutting from TARS 1881

text: TARS 1881

text: TARS 1881


text: Cornell University

text: public

text: Kyle Wallick

text: poinsettia

characteristic: common name

text: Leaf from 2016-0302 (USA: USBG)

text: USA

text: Garden, USBG

text: Kyle Wallick

text: Euphorbia pulcherrima

text: Poinsettia

characteristic: organism common name

text: soil

characteristic: plant growth medium

text: Leaves

characteristic: plant structure

text: USBG

characteristic: sample storage location

text: Euphorbia pulcherrima

text: Plant at USBG

characteristic: source material description


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cornell University

text: public

text: Mike deMotta

text: Leaf from 880627 (USA: NTBG)

text: USA

text: Greenhouse, NTBG

text: Hideo Teshima

text: Excoecaria cochinchinensis

text: Blindness tree

text: soil

text: Leaves

text: NTBG

text: Excoecaria cochinchinensis

text: Plant at NTBG

characteristic: source material identifiers

characteristic: title


text: Cancer Research UK Cambridge Institute.

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Cancer Research UK Cambridge Institute.

text: ArrayExpress

characteristic: broker name

text: house mouse

text: Protocols: Mouse embryos from the appropriate somite stages (8 to 35 somites) were dissected in RNAse-free conditions in cold PBS, on silicone plates. To dissect out the somites, embryos were treated with dispase II (1mg/mL in DMEM) for 30-45 seconds at 37°C. The three most posterior pairs of somites were then collected using tungsten needles, dissecting out every somite separately. We labelled each somite pair as somite I, II or III, from the most posterior to the most anterior, respectively. Each individual somite was placed in 10µL of lysis buffer (Takara) containing RNase inhibitor. One somite from each pair was flash frozen in liquid nitrogen and stored at -80°C for later processing for RNA-seq. The matching somites were directly processed to generate ATAC-seq libraries. Reverse transcription was performed directly on frozen lysed somites and cDNA was amplified with 8 cycles of PCR. RNA-seq libraries were generated using 100pg of amplified cDNA with the SMART-seq v4 Ultra Low Input RNA Kit for Sequencing (TaKaRa, 634891) and the NEXTERA XT DNA Library Preparation kit (Illumina, FC-131-1096), according to the manufacturer’s instructions, except only a quarter of the recommended reagents’ amount was used. The resulting libraries were quantified using a Qubit instrument and their size distributions were assessed with a TapeStation machine.

characteristic: description

text: embryo stage

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

characteristic: individual

text: Mus musculus

characteristic: organism

text: Mus musculus

text: somite 8

characteristic: organism part

text: posterior

characteristic: sampling site

text: Mus musculus

text: male

characteristic: sex

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cancer Research UK Cambridge Institute.

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

characteristic: common name

text: embryo stage

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: somite 8

text: medial

text: Mus musculus

text: male


text: Cancer Research UK Cambridge Institute.

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

characteristic: broker name

text: house mouse

text: embryo stage

text: wild type genotype

text: Mus musculus

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