text: NVRL

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text: Europe / Ireland / Meath

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text: hCov-19 Oxford Nanopore MinION reads obtained following the Artic v3 protocol

text: Ireland

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characteristic: geographic location (latitude)

text: Europe / Ireland / Meath

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text: hCoV-19/Ireland/MH-NVRL-SIRL00015567/2023


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text: hCoV-19/Ireland/MN-NVRL-SIRL00016385/2023


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text: Europe / Ireland / Mayo

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text: hCoV-19/Ireland/MO-NVRL-SIRL00015512/2023


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text: NVRL

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text: hCoV-19/Ireland/SO-NVRL-SIRL00015699/2023


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text: hCoV-19/Ireland/TA-NVRL-SIRL00016488/2023


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text: hCoV-19/Ireland/TA-NVRL-SIRL00015569/2023


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text: Homo sapiens

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characteristic: organism

text: hCoV-19/Ireland/TA-NVRL-SIRL00015694/2023


text: NVRL

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characteristic: collector name

text: Human coronavirus 2019

text: hCov-19 Oxford Nanopore MinION reads obtained following the Artic v3 protocol

text: Ireland

characteristic: geographic location (latitude)

text: Europe / Ireland / Tipperary

text: human

text: not provided

text: not provided

text: Homo sapiens

text: male

text: not provided

text: Oro-Nasopharyngeal swab

characteristic: organism

text: hCoV-19/Ireland/TA-NVRL-SIRL00015686/2023


text: NVRL

text: public

text: Irish Consortium for Sequencing Covid

text: National Virus Reference Laboratory

text: not provided

text: Human coronavirus 2019

characteristic: common name

characteristic: description

text: Ireland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (longitude)

text: Europe / Ireland / Waterford

characteristic: geographic location (region and locality)

characteristic: host age

text: human

characteristic: host common name

text: not provided

characteristic: host disease outcome

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: female

characteristic: host sex

text: not provided

characteristic: host subject id

text: Oro-Nasopharyngeal swab

characteristic: isolation source host-associated

text: Severe acute respiratory syndrome coronavirus 2

characteristic: sample capture status

text: hCoV-19/Ireland/WD-NVRL-SIRL00016628/2023

characteristic: title

characteristic: virus identifier


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NVRL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Irish Consortium for Sequencing Covid

characteristic: broker name

text: National Virus Reference Laboratory

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Human coronavirus 2019

text: Ireland

characteristic: geographic location (latitude)

text: Europe / Ireland / Waterford

text: human

text: not provided

text: not provided

text: Homo sapiens

text: female

text: not provided

text: Oro-Nasopharyngeal swab

characteristic: organism

text: hCoV-19/Ireland/WD-NVRL-SIRL00016627/2023


text: NVRL

text: public

text: Irish Consortium for Sequencing Covid

text: National Virus Reference Laboratory

text: not provided

text: Human coronavirus 2019

characteristic: common name

characteristic: description

text: Ireland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (longitude)

text: Europe / Ireland / Waterford

characteristic: geographic location (region and locality)

characteristic: host age

text: human

characteristic: host common name

text: not provided

characteristic: host disease outcome

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: female

characteristic: host sex

text: not provided

characteristic: host subject id

text: Oro-Nasopharyngeal swab

characteristic: isolation source host-associated

characteristic: sample capture status

text: hCoV-19/Ireland/WD-NVRL-SIRL00016715/2023

characteristic: title

characteristic: virus identifier


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NVRL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Irish Consortium for Sequencing Covid

characteristic: broker name

text: National Virus Reference Laboratory

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Human coronavirus 2019

text: Ireland

characteristic: geographic location (latitude)

text: Europe / Ireland / Waterford

text: human

text: not provided

text: not provided

text: Homo sapiens

text: male

text: not provided

text: Oro-Nasopharyngeal swab

characteristic: organism

text: hCoV-19/Ireland/WD-NVRL-SIRL00015476/2023


text: NVRL

text: public

text: Irish Consortium for Sequencing Covid

text: National Virus Reference Laboratory

text: not provided

text: Human coronavirus 2019

characteristic: common name

characteristic: description

text: Ireland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (longitude)

text: Europe / Ireland / Waterford

characteristic: geographic location (region and locality)

characteristic: host age

text: human

characteristic: host common name

text: not provided

characteristic: host disease outcome

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: female

characteristic: host sex

text: not provided

characteristic: host subject id

text: Oro-Nasopharyngeal swab

characteristic: isolation source host-associated

characteristic: sample capture status

text: hCoV-19/Ireland/WD-NVRL-SIRL00015854/2023

characteristic: title

characteristic: virus identifier


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NVRL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Irish Consortium for Sequencing Covid

characteristic: broker name

text: National Virus Reference Laboratory

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Human coronavirus 2019

text: Ireland

characteristic: geographic location (latitude)

text: Europe / Ireland / Westmeath

text: human

text: not provided

text: not provided

text: Homo sapiens

text: female

text: not provided

text: Oro-Nasopharyngeal swab

characteristic: organism

text: hCoV-19/Ireland/WH-NVRL-SIRL00016676/2023


text: NVRL

text: public

text: Irish Consortium for Sequencing Covid

text: National Virus Reference Laboratory

text: not provided

text: Human coronavirus 2019

characteristic: common name

characteristic: description

text: Ireland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (longitude)

text: Europe / Ireland / Westmeath

characteristic: geographic location (region and locality)

characteristic: host age

text: human

characteristic: host common name

text: not provided

characteristic: host disease outcome

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: male

characteristic: host sex

text: not provided

characteristic: host subject id

text: Oro-Nasopharyngeal swab

characteristic: isolation source host-associated

characteristic: sample capture status

text: hCoV-19/Ireland/WH-NVRL-SIRL00015539/2023

characteristic: title

characteristic: virus identifier


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NVRL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Irish Consortium for Sequencing Covid

characteristic: broker name

text: National Virus Reference Laboratory

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Human coronavirus 2019

text: Ireland

characteristic: geographic location (latitude)

text: Europe / Ireland / Westmeath

text: human

text: not provided

text: not provided

text: Homo sapiens

text: female

text: not provided

text: Oro-Nasopharyngeal swab

characteristic: organism

text: hCoV-19/Ireland/WH-NVRL-SIRL00015548/2023


text: NVRL

text: public

text: Irish Consortium for Sequencing Covid

text: National Virus Reference Laboratory

text: not provided

text: Human coronavirus 2019

characteristic: common name

characteristic: description

text: Ireland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (longitude)

text: Europe / Ireland / Westmeath

characteristic: geographic location (region and locality)

characteristic: host age

text: human

characteristic: host common name

text: not provided

characteristic: host disease outcome

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: male

characteristic: host sex

text: not provided

characteristic: host subject id

text: Oro-Nasopharyngeal swab

characteristic: isolation source host-associated

characteristic: sample capture status

text: hCoV-19/Ireland/WH-NVRL-SIRL00015554/2023

characteristic: title

characteristic: virus identifier


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NVRL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Irish Consortium for Sequencing Covid

characteristic: broker name

text: National Virus Reference Laboratory

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Human coronavirus 2019

text: Ireland

characteristic: geographic location (latitude)

text: Europe / Ireland / Westmeath

text: human

text: not provided

text: not provided

text: Homo sapiens

text: male

text: not provided

text: Oro-Nasopharyngeal swab

characteristic: organism

text: hCoV-19/Ireland/WH-NVRL-SIRL00015555/2023


text: NVRL

text: public

text: Irish Consortium for Sequencing Covid

text: National Virus Reference Laboratory

text: not provided

text: Human coronavirus 2019

characteristic: common name

characteristic: description

text: Ireland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (longitude)

text: Europe / Ireland / Westmeath

characteristic: geographic location (region and locality)

characteristic: host age

text: human

characteristic: host common name

text: not provided

characteristic: host disease outcome

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: female

characteristic: host sex

text: not provided

characteristic: host subject id

text: Oro-Nasopharyngeal swab

characteristic: isolation source host-associated

characteristic: sample capture status

text: hCoV-19/Ireland/WH-NVRL-SIRL00015549/2023

characteristic: title

characteristic: virus identifier


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NVRL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Irish Consortium for Sequencing Covid

characteristic: broker name

text: National Virus Reference Laboratory

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Human coronavirus 2019

text: Ireland

characteristic: geographic location (latitude)

text: Europe / Ireland / Wicklow

text: human

text: not provided

text: not provided

text: Homo sapiens

text: female

text: not provided

text: Oro-Nasopharyngeal swab

characteristic: organism

text: hCoV-19/Ireland/WW-NVRL-SIRL00016548/2023


text: NVRL

text: public

text: Irish Consortium for Sequencing Covid

text: National Virus Reference Laboratory

text: not provided

text: Human coronavirus 2019

characteristic: common name

characteristic: description

text: Ireland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (longitude)

text: Europe / Ireland / Wicklow

characteristic: geographic location (region and locality)

characteristic: host age

text: human

characteristic: host common name

text: not provided

characteristic: host disease outcome

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: female

characteristic: host sex

text: not provided

characteristic: host subject id

text: Oro-Nasopharyngeal swab

characteristic: isolation source host-associated

text: active surveillance in response to outbreak

characteristic: sample capture status

text: hCoV-19/Ireland/WW-NVRL-SIRL00016555/2023

characteristic: title

characteristic: virus identifier


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NVRL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Irish Consortium for Sequencing Covid

characteristic: broker name

text: National Virus Reference Laboratory

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Human coronavirus 2019

text: hCov-19 Oxford Nanopore MinION reads obtained following the Artic v3 protocol

text: Ireland

characteristic: geographic location (latitude)

text: Europe / Ireland / Wicklow

text: human

text: not provided

text: not provided

text: Homo sapiens

text: female

text: not provided

text: Oro-Nasopharyngeal swab

characteristic: organism

text: hCoV-19/Ireland/WW-NVRL-SIRL00016549/2023


text: NVRL

text: public

text: Irish Consortium for Sequencing Covid

text: National Virus Reference Laboratory

text: not provided

text: Human coronavirus 2019

characteristic: common name

characteristic: description

text: Ireland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (longitude)

text: Europe / Ireland / Wicklow

characteristic: geographic location (region and locality)

characteristic: host age

text: human

characteristic: host common name

text: not provided

characteristic: host disease outcome

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: female

characteristic: host sex

text: not provided

characteristic: host subject id

text: Oro-Nasopharyngeal swab

characteristic: isolation source host-associated

text: Severe acute respiratory syndrome coronavirus 2

characteristic: sample capture status

text: hCoV-19/Ireland/WW-NVRL-SIRL00016473/2023

characteristic: title

characteristic: virus identifier


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NVRL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Irish Consortium for Sequencing Covid

characteristic: broker name

text: National Virus Reference Laboratory

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Human coronavirus 2019

text: Ireland

characteristic: geographic location (latitude)

text: Europe / Ireland / Wicklow

text: human

text: not provided

text: not provided

text: Homo sapiens

text: female

text: not provided

text: Oro-Nasopharyngeal swab

characteristic: organism

text: hCoV-19/Ireland/WW-NVRL-SIRL00016340/2023


text: NVRL

text: public

text: Irish Consortium for Sequencing Covid

text: National Virus Reference Laboratory

text: not provided

text: Human coronavirus 2019

characteristic: common name

characteristic: description

text: Ireland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (longitude)

text: Europe / Ireland / Wicklow

characteristic: geographic location (region and locality)

characteristic: host age

text: human

characteristic: host common name

text: not provided

characteristic: host disease outcome

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: female

characteristic: host sex

text: not provided

characteristic: host subject id

text: Oro-Nasopharyngeal swab

characteristic: isolation source host-associated

characteristic: sample capture status

text: hCoV-19/Ireland/WW-NVRL-SIRL00016267/2023

characteristic: title

characteristic: virus identifier


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NVRL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Irish Consortium for Sequencing Covid

characteristic: broker name

text: National Virus Reference Laboratory

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Human coronavirus 2019

text: Ireland

characteristic: geographic location (latitude)

text: Europe / Ireland / Wicklow

text: human

text: not provided

text: not provided

text: Homo sapiens

text: female

text: not provided

text: Oro-Nasopharyngeal swab

characteristic: organism

text: hCoV-19/Ireland/WW-NVRL-SIRL00015793/2023


text: NVRL

text: public

text: Irish Consortium for Sequencing Covid

text: National Virus Reference Laboratory

text: not provided

text: Human coronavirus 2019

characteristic: common name

characteristic: description

text: Ireland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (longitude)

text: Europe / Ireland / Wicklow

characteristic: geographic location (region and locality)

characteristic: host age

text: human

characteristic: host common name

text: not provided

characteristic: host disease outcome

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: female

characteristic: host sex

text: not provided

characteristic: host subject id

text: Oro-Nasopharyngeal swab

characteristic: isolation source host-associated

characteristic: sample capture status

text: hCoV-19/Ireland/WW-NVRL-SIRL00015795/2023

characteristic: title

characteristic: virus identifier


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NVRL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Irish Consortium for Sequencing Covid

characteristic: broker name

text: National Virus Reference Laboratory

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Human coronavirus 2019

text: Ireland

characteristic: geographic location (latitude)

text: Europe / Ireland / Wexford

text: human

text: not provided

text: not provided

text: Homo sapiens

text: male

text: not provided

text: Oro-Nasopharyngeal swab

characteristic: organism

text: hCoV-19/Ireland/WX-NVRL-SIRL00016738/2023


text: NVRL

text: public

text: Irish Consortium for Sequencing Covid

text: National Virus Reference Laboratory

text: not provided

text: Human coronavirus 2019

characteristic: common name

characteristic: description

text: Ireland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (longitude)

text: Europe / Ireland / Wexford

characteristic: geographic location (region and locality)

characteristic: host age

text: human

characteristic: host common name

text: not provided

characteristic: host disease outcome

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: female

characteristic: host sex

text: not provided

characteristic: host subject id

text: Oro-Nasopharyngeal swab

characteristic: isolation source host-associated

characteristic: sample capture status

text: hCoV-19/Ireland/WX-NVRL-SIRL00016759/2023

characteristic: title

characteristic: virus identifier


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NVRL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Irish Consortium for Sequencing Covid

characteristic: broker name

text: National Virus Reference Laboratory

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Human coronavirus 2019

text: Ireland

characteristic: geographic location (latitude)

text: Europe / Ireland / Wexford

text: human

text: not provided

text: not provided

text: Homo sapiens

text: male

text: not provided

text: Oro-Nasopharyngeal swab

characteristic: organism

text: hCoV-19/Ireland/WX-NVRL-SIRL00016276/2023


text: NVRL

text: public

text: Irish Consortium for Sequencing Covid

text: National Virus Reference Laboratory

text: not provided

text: Human coronavirus 2019

characteristic: common name

characteristic: description

text: Ireland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (longitude)

text: Europe / Ireland / Wexford

characteristic: geographic location (region and locality)

characteristic: host age

text: human

characteristic: host common name

text: not provided

characteristic: host disease outcome

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: male

characteristic: host sex

text: not provided

characteristic: host subject id

text: Oro-Nasopharyngeal swab

characteristic: isolation source host-associated

characteristic: sample capture status

text: hCoV-19/Ireland/WX-NVRL-SIRL00015592/2023

characteristic: title

characteristic: virus identifier


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NVRL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Irish Consortium for Sequencing Covid

characteristic: broker name

text: National Virus Reference Laboratory

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Human coronavirus 2019

text: Ireland

characteristic: geographic location (latitude)

text: Europe / Ireland / Wexford

text: human

text: not provided

text: not provided

text: Homo sapiens

text: female

text: not provided

text: Oro-Nasopharyngeal swab

characteristic: organism

text: hCoV-19/Ireland/WX-NVRL-SIRL00015790/2023


characteristic: GISAID Accession ID

text: public

tag: Namespace:Institute for Infectious Diseases, Bern, Switzerland

text: SIB

text: Institute for Infectious Diseases, University of Bern, Switzerland

text: Stefan Neuenschwander, Christian Baumann, Miguel A Terrazos Miani, Sonja Gempeler, Loïc Borcard, Lea Stauber, Cora Sägesser, Pascal Bittel, Peter Keller, Franziska Suter-Riniker, Stephen L Leib, Alban Ramette

text: Switzerland

characteristic: geographic location (country and/or sea)

text: BE

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/BE-IFIK-230327_os_06/2023

characteristic: isolate

characteristic: sample capture status

text: active surveillance not initiated by an outbreak

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Institute for Infectious Diseases, Bern, Switzerland

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: broker name

text: Switzerland

text: BE

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/BE-IFIK-230327_os_31/2023

text: active surveillance in response to outbreak


text: The Francis Crick Insitute

text: public

tag: Namespace:The Francis Crick Insitute

text: Shotgun sequenced metagenome with human reads aligning to Hg37 removed

characteristic: description

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Yersinia pestis

characteristic: organism

text: Yersinia pestis

text: C10091 Shotgun


C10098 Capture

text: The Francis Crick Insitute

text: public

text: C10098 Capture

text: Capture enriched ancient genomic Yersinia pestis using commercially available myBaits Arbor Biosciences, following myBaits custom RNA seq v5.1 (March 2021) High Sensitivity protocol

text: United Kingdom

text: Yersinia pestis

text: Yersinia pestis

text: C10098 Capture


text: The Francis Crick Insitute

text: public

text: United Kingdom

text: Yersinia pestis

text: Yersinia pestis

text: C10098 Shotgun


C10928 Capture

text: The Francis Crick Insitute

text: public

text: C10928 Capture

text: United Kingdom

text: Yersinia pestis

text: Yersinia pestis

text: C10928 Capture


text: James Hutton Institute

text: public

tag: Namespace:James Hutton Institute

text: Illumina RenSeq of accession blb7637

text: Solanum bulbocastanum

text: Solanum bulbocastanum


text: James Hutton Institute

text: public

text: Illumina RenSeq of accession blb7641

text: Solanum bulbocastanum

text: Solanum bulbocastanum


text: James Hutton Institute

text: public

text: Illumina RenSeq of accession blb7644

text: Solanum bulbocastanum

text: Solanum bulbocastanum


text: public

tag: Namespace:INSTITUTE OF APPLIED BIOSCIENCES - CERTH

text: Department of Microbiology, Medical School, Aristotle University of Thessaloniki, Thessaloniki, Greece

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

characteristic: common name

text: Greece

text: Thessaloniki

characteristic: geographic location (region and locality)

text: human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

characteristic: host subject id

text: RNA

characteristic: isolate

text: Severe acute respiratory syndrome coronavirus 2


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INSTITUTE OF APPLIED BIOSCIENCES - CERTH

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: not provided

text: Greece

text: Thessaloniki

text: human

text: not provided

text: Homo sapiens

text: not provided

text: RNA

characteristic: sample capture status

characteristic: title


text: public

characteristic: Submitter Id

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

characteristic: common name

characteristic: description

text: Greece

characteristic: geographic location (country and/or sea)

text: Chalkidiki

characteristic: geographic location (region and locality)

text: human

characteristic: host common name

text: not provided

text: Homo sapiens

text: not provided

text: RNA

characteristic: organism


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: not provided

text: Greece

text: Serres

text: human

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

characteristic: host subject id

text: RNA

characteristic: isolate


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Greece

text: Evros

text: human

text: not provided

text: Homo sapiens

text: not provided

text: RNA

characteristic: sample capture status

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: not provided

characteristic: common name

characteristic: description

text: Greece

characteristic: geographic location (country and/or sea)

text: Pella

characteristic: geographic location (region and locality)

text: human

characteristic: host common name

text: not provided

text: Homo sapiens

text: not provided

text: RNA

characteristic: organism


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: not provided

text: Greece

text: Drama

text: human

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

characteristic: host subject id

text: RNA

characteristic: isolate


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: Submitter Id

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Greece

text: Xanthi

text: human

text: not provided

text: Homo sapiens

text: not provided

text: RNA

characteristic: sample capture status

characteristic: title


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: not provided

characteristic: common name

characteristic: description

text: Greece

characteristic: geographic location (country and/or sea)

text: Kria Vrisi

characteristic: geographic location (region and locality)

text: human

characteristic: host common name

text: not provided

text: Homo sapiens

text: not provided

text: RNA

characteristic: organism


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: James Hutton Institute

text: public

characteristic: SRA accession

text: Illumina RenSeq of accession blb7638

text: Solanum bulbocastanum

text: Solanum bulbocastanum


text: James Hutton Institute

text: public

characteristic: Submitter Id

text: Illumina RenSeq of accession blb7642

text: Solanum bulbocastanum

text: Solanum bulbocastanum


text: James Hutton Institute

text: public

text: Illumina RenSeq of accession blb7643

text: Solanum bulbocastanum

text: Solanum bulbocastanum

characteristic: title


text: James Hutton Institute

characteristic: INSDC center name

text: public

text: Illumina RenSeq of accession blb7645

text: Solanum bulbocastanum

text: Solanum bulbocastanum


text: James Hutton Institute

characteristic: INSDC first public

text: public

text: Illumina RenSeq of accession blb7646

text: Solanum bulbocastanum

text: Solanum bulbocastanum


text: James Hutton Institute

characteristic: INSDC last update

text: public

text: Illumina RenSeq of accession blb7650

text: Solanum bulbocastanum

text: Solanum bulbocastanum


text: James Hutton Institute

text: public

characteristic: INSDC status

text: Illumina RenSeq of accession blb7651

text: Solanum bulbocastanum

text: Solanum bulbocastanum


text: PNRI

text: public

text: baker's yeast

characteristic: common name

text: Pooled sequencing of yOTC to identify variant codons

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: public

tag: Namespace:West of Scotland Specialist Virology Centre, NHSGGC / MRC-University of Glasgow Centre for Virus Research

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

text: Scotland

text: not provided

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: not provided

characteristic: virus identifier


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: West of Scotland Specialist Virology Centre, NHSGGC / MRC-University of Glasgow Centre for Virus Research

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: not provided

text: not provided

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Scotland

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: organism

text: not provided

characteristic: title

text: not provided


text: GENGS-CBMSO

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: GENGS-CBMSO

characteristic: broker name

text: house mouse

characteristic: common name

text: RNAseq of the cerebral cortex of wild type mices with normal metabolic state

characteristic: description

text: Mus musculus

text: Mus musculus


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: house mouse

text: RNAseq of the cerebral cortex of human amyloid peptide-expressing mice ( NL/F) affected by T2DM with normal metabolic state

text: Mus musculus

text: Mus musculus


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

tag: Namespace:Biodiversity Research Centre, Academia Sinica

text: 2 L

characteristic: amount or size of sample collected

characteristic: annotation source

text: aquatic environment [ENVO:01000317]

characteristic: broad-scale environmental context

characteristic: collection date

text: 0 meter

characteristic: depth

text: Upstream, Houdong waterfall

text: liquid water [ENVO:00002006]

characteristic: environmental medium

text: sampling site [EFO:0000688]

characteristic: experimental factor

text: Taiwan

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Yilan County, Houdong river

text: waterfall [ENVO:00000040]

characteristic: local environmental context

text: FWD:GCGACAAGCACCG,REV:CTGAGGTGATCAG

characteristic: multiplex identifiers

text: Presto™ Stool gDNA Extraction Kit Manual Ver. 10.21.17 | https://www.geneaid.com/data/files/1605749978971961484.pdf

characteristic: nucleic acid extraction

text: metagenome

characteristic: pH

text: starts with 95°C for 2 min, followed by 5 cycles of 95°C for 1 min, 46°C for 1 min, and 72°C for 30 sec, followed by 35 cycles of 95°C for 1 min, 53°C for 1 min, and 72°C for 30 sec, and final extension at 72°C for 5 min

characteristic: pcr conditions

text: FWD:TCCACTAATCACAARGATATTGGTAC,REV:GAAAATCATAATGAAGGCATGAGC

characteristic: pcr primers

text: Environmental DNA-based biodiversity profiling along Houdong riverine zonation in Taiwan

characteristic: project name

characteristic: salinity

text: 500 mL

characteristic: sample volume or weight for DNA extraction

text: metagenome

text: software

characteristic: sequence quality check

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: cytochrome oxidase subunit 1 (COI) mitochondrial gene

characteristic: target gene

characteristic: temperature

text: waterfall 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Biodiversity Research Centre, Academia Sinica

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 2 L

characteristic: amount or size of sample collected

characteristic: annotation source

text: aquatic environment [ENVO:01000317]

characteristic: broad-scale environmental context

characteristic: collection date

text: 0 meter

characteristic: depth

text: Upstream, Houdong waterfall

characteristic: description

text: liquid water [ENVO:00002006]

characteristic: environmental medium

text: sampling site [EFO:0000688]

text: Taiwan

characteristic: geographic location (country and/or sea)

text: Yilan County, Houdong river

characteristic: geographic location (region and locality)

text: waterfall [ENVO:00000040]

text: FWD:GCGACAAGCACCG,REV:AGTGTCCTGCTAG

text: metagenome

characteristic: organism

text: 500 mL

text: metagenome

text: software

text: Illumina NovaSeq 6000

text: waterfall 2

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: 2 L

text: aquatic environment [ENVO:01000317]

text: 0 meter

text: Upstream, Houdong waterfall

text: liquid water [ENVO:00002006]

text: sampling site [EFO:0000688]

characteristic: experimental factor

text: Taiwan

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Yilan County, Houdong river

text: waterfall [ENVO:00000040]

characteristic: local environmental context

text: FWD:GCGACAAGCACCG,REV:ATAAGCAATTCGA

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: pH

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

characteristic: salinity

text: 500 mL

characteristic: sample volume or weight for DNA extraction

text: metagenome

text: software

characteristic: sequence quality check

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: target gene

characteristic: temperature

text: waterfall 3


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 2 L

characteristic: amount or size of sample collected

characteristic: annotation source

text: aquatic environment [ENVO:01000317]

characteristic: broad-scale environmental context

characteristic: collection date

text: 0 meter

characteristic: depth

text: Downstream, Houdong river

characteristic: description

text: liquid water [ENVO:00002006]

characteristic: environmental medium

text: sampling site [EFO:0000688]

text: Taiwan

characteristic: geographic location (country and/or sea)

text: Yilan County, Houdong river

characteristic: geographic location (region and locality)

text: freshwater river [ENVO:01000297]

text: FWD:GCGACAAGCACCG,REV:ATCTAACAGGACC

text: metagenome

characteristic: organism

text: 500 mL

text: metagenome

text: software

text: Illumina NovaSeq 6000

text: freshwater river 1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: 2 L

text: aquatic environment [ENVO:01000317]

text: 0 meter

text: Downstream, Houdong river

text: liquid water [ENVO:00002006]

text: sampling site [EFO:0000688]

characteristic: experimental factor

text: Taiwan

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Yilan County, Houdong river

text: freshwater river [ENVO:01000297]

characteristic: local environmental context

text: FWD:GCGACAAGCACCG,REV:CATTATATAGCCA

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: pH

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

characteristic: salinity

text: 500 mL

characteristic: sample volume or weight for DNA extraction

text: metagenome

text: software

characteristic: sequence quality check

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: target gene

characteristic: temperature

text: freshwater river 2


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 2 L

characteristic: amount or size of sample collected

characteristic: annotation source

text: aquatic environment [ENVO:01000317]

characteristic: broad-scale environmental context

characteristic: collection date

text: 0 meter

characteristic: depth

text: Downstream, Houdong river

characteristic: description

text: liquid water [ENVO:00002006]

characteristic: environmental medium

text: sampling site [EFO:0000688]

text: Taiwan

characteristic: geographic location (country and/or sea)

text: Yilan County, Houdong river

characteristic: geographic location (region and locality)

text: freshwater river [ENVO:01000297]

text: FWD:GCGACAAGCACCG,REV:CCTGATTACGTAA

text: metagenome

characteristic: organism

text: 500 mL

text: metagenome

text: software

text: Illumina NovaSeq 6000

text: freshwater river 3

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: 2 L

text: aquatic environment [ENVO:01000317]

text: 0 meter

text: Semiseawater, Xiapu

text: liquid water [ENVO:00002006]

text: sampling site [EFO:0000688]

characteristic: experimental factor

text: Taiwan

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Yilan County, Houdong river

text: estuary [ENVO:00000045]

characteristic: local environmental context

text: FWD:GCGACAAGCACCG,REV:CTAATACACACCG

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: pH

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

characteristic: salinity

text: 500 mL

characteristic: sample volume or weight for DNA extraction

text: metagenome

text: software

characteristic: sequence quality check

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: target gene

characteristic: temperature

text: estuary 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 2 L

characteristic: amount or size of sample collected

characteristic: annotation source

text: aquatic environment [ENVO:01000317]

characteristic: broad-scale environmental context

characteristic: collection date

text: 0 meter

characteristic: depth

text: Semiseawater, Xiapu

characteristic: description

text: liquid water [ENVO:00002006]

characteristic: environmental medium

text: sampling site [EFO:0000688]

text: Taiwan

characteristic: geographic location (country and/or sea)

text: Yilan County, Houdong river

characteristic: geographic location (region and locality)

text: estuary [ENVO:00000045]

text: FWD:GCGACAAGCACCG,REV:TTATGACCGTTGC

text: metagenome

characteristic: organism

text: 500 mL

text: metagenome

text: software

text: Illumina NovaSeq 6000

text: estuary 2

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: 2 L

text: aquatic environment [ENVO:01000317]

text: 0 meter

text: Semiseawater, Xiapu

text: liquid water [ENVO:00002006]

text: sampling site [EFO:0000688]

characteristic: experimental factor

text: Taiwan

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Yilan County, Houdong river

text: estuary [ENVO:00000045]

characteristic: local environmental context

text: FWD:GCGACAAGCACCG,REV:TTCTCGGCCGCAT

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: pH

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

characteristic: salinity

text: 500 mL

characteristic: sample volume or weight for DNA extraction

text: metagenome

text: software

characteristic: sequence quality check

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: target gene

characteristic: temperature

text: estuary 3


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 2 L

characteristic: amount or size of sample collected

characteristic: annotation source

text: aquatic environment [ENVO:01000317]

characteristic: broad-scale environmental context

characteristic: collection date

text: 0 meter

characteristic: depth

text: Seawater, Zhu An river mouth

characteristic: description

text: liquid water [ENVO:00002006]

characteristic: environmental medium

text: sampling site [EFO:0000688]

text: Taiwan

characteristic: geographic location (country and/or sea)

text: Yilan County, Houdong river

characteristic: geographic location (region and locality)

text: river mouth [ENVO:00000386]

text: FWD:GCGACAAGCACCG,REV:TTGGCGGTAAGAA

text: metagenome

characteristic: organism

text: 500 mL

text: metagenome

text: software

text: Illumina NovaSeq 6000

text: river mouth 1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: 2 L

text: aquatic environment [ENVO:01000317]

text: 0 meter

text: Seawater, Zhu An river mouth

text: liquid water [ENVO:00002006]

text: sampling site [EFO:0000688]

characteristic: experimental factor

text: Taiwan

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Yilan County, Houdong river

text: river mouth [ENVO:00000386]

characteristic: local environmental context

text: FWD:GCGACAAGCACCG,REV:ACTTGTGCACCTG

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: pH

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

characteristic: salinity

text: 500 mL

characteristic: sample volume or weight for DNA extraction

text: metagenome

text: software

characteristic: sequence quality check

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: target gene

characteristic: temperature

text: river mouth 2


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 2 L

characteristic: amount or size of sample collected

characteristic: annotation source

text: aquatic environment [ENVO:01000317]

characteristic: broad-scale environmental context

characteristic: collection date

text: 0 meter

characteristic: depth

text: Seawater, Zhu An river mouth

characteristic: description

text: liquid water [ENVO:00002006]

characteristic: environmental medium

text: sampling site [EFO:0000688]

text: Taiwan

characteristic: geographic location (country and/or sea)

text: Yilan County, Houdong river

characteristic: geographic location (region and locality)

text: river mouth [ENVO:00000386]

text: FWD:GCGACAAGCACCG,REV:CATGCCTTGATAC

text: metagenome

characteristic: organism

text: 500 mL

text: metagenome

text: software

text: Illumina NovaSeq 6000

text: river mouth 3

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

tag: Namespace:Senckenber Biodiversity and Climate Research Centre Frankfurt

text: European beech

characteristic: common name

text: pool of trees with stem from population KST

text: Fagus sylvatica

text: Fagus sylvatica


text: Senckenber Biodiversity and Climate Research Centre Frankfurt

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: European beech

text: pool of young trees from population KST

text: Fagus sylvatica

text: Fagus sylvatica


text: public

text: European beech

text: pool of intermediated trees (pole) from population KST

characteristic: description

text: Fagus sylvatica

text: Fagus sylvatica


text: public

text: European beech

text: pool of trees with stem from population LAN

text: Fagus sylvatica

text: Fagus sylvatica


text: public

text: European beech

text: pool of young trees from population LAN

text: Fagus sylvatica

text: Fagus sylvatica


text: public

text: European beech

text: pool of intermediated trees (pole) from population LAN

text: Fagus sylvatica

text: Fagus sylvatica


text: public

text: European beech

text: pool of trees from population �stringen

text: Fagus sylvatica

text: Fagus sylvatica


text: public

text: European beech

text: pool of trees from population Marxzell

text: Fagus sylvatica

text: Fagus sylvatica


text: public

text: European beech

text: pool of trees from population Langenbrettach

text: Fagus sylvatica

text: Fagus sylvatica


text: public

text: European beech

text: pool of trees from population Tauberbischofsheim

text: Fagus sylvatica

text: Fagus sylvatica


text: public

text: European beech

text: pool of trees from population Kaufbeuren

text: Fagus sylvatica

text: Fagus sylvatica


text: public

text: European beech

text: pool of trees from population Seilershausen

text: Fagus sylvatica

text: Fagus sylvatica


text: public

text: European beech

text: pool of trees from population Ulm

text: Fagus sylvatica

text: Fagus sylvatica


text: public

text: European beech

text: pool of trees from population Karlstadt (Arnstein)

text: Fagus sylvatica

text: Fagus sylvatica


text: public

text: European beech

text: pool of trees from population Wurzelberg

text: Fagus sylvatica

text: Fagus sylvatica


text: public

text: European beech

text: pool of trees from population Cunnersdorf

text: Fagus sylvatica

text: Fagus sylvatica


text: public

text: European beech

text: pool of trees from population Mockrehna (GB)

text: Fagus sylvatica

text: Fagus sylvatica


text: public

text: European beech

text: pool of trees from population Mockrehna (marginal)

text: Fagus sylvatica

text: Fagus sylvatica


text: public

text: European beech

text: pool of trees from population Ottendorf

text: Fagus sylvatica

text: Fagus sylvatica


text: public

tag: Namespace:Center for Cancer Research, Medical University of Vienna, Comprehensive Cancer Center

text: ArrayExpress

characteristic: broker name

text: keratinocyte

text: house mouse

text: Protocols: All strains used were in C57BL6/J background, only females were used, dox diet was started at age of 7 weeks. Genotypes: K5-ILEIind: K5rtTA/+;ILEIind/ILEIind, ILEIind littermate ctrl: +/+;ILEIind/ILEIind. Treatment: doxocycline diet(1000mg/kg) started 3 days before the 5-days TPA treatment was started. Day 6 after TPA start mice were sacrificed, total skin taken, RNA extracted and analyzed. For 24h dataset, doxycycline start same, say -3,then TPA one day, 24h later sacrifice, keratinocytes FACS sorted, RNA prep and for seq. For tissue homogenization, back skin was mechanically disrupted with a Precellys 24 homogenizer (Bertin). Single-cell suspensions of freshly isolated keratinocytes were prepared from epidermal sheet as described above and subsequently blocked with anti-CD16/32 antibody (BioLegend). Cells were stained with APC-conjugated CD45 and FITC-conjugated Ly6a/Sca1 antibodies (Biolegend) for 30 min at 4°C. After incubation, cells were washed, filtered, and stained with Zombie UVTM viability dye (BioLegend) according to the manufacturer’s recommendation to exclude dead cells. Keratinocytes were gated as CD45 negative live cell population, and sorted to be enriched for cells of the interfollicular epithelium (IFE; Ly6a/Sca1 positive) and of hair follicles (HF; Ly6a/Sca1 negative) according to (Sakamoto et al, 2022) by a Moflo Astrios EQ cell sorter (Beckman Coulter) at cooled conditions. Sorted cells were directly pelleted and propagated for RNA isolation. RNA was extracted from murine back skin and from freshly isolated or cultured primary keratinocytes by using TRIzol reagent (Invitrogen). The directional library was prepared using NEBNext® UltraTM Directional RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s protocol. Indices were included to multiplex multiple samples. Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The strand-specific library was ready after end repair, A-tailing, adapter ligation, size selection, and USER enzyme digestion. After amplification and purification, insert size of the library was validated on an Agilent 2100 and quantified using quantitative PCR (Q-PCR).

text: adult

characteristic: developmental stage

text: psoriasis

characteristic: disease

characteristic: genotype

text: CD45 positive

characteristic: immunophenotype

text: Mus musculus

text: Mus musculus

text: skin

characteristic: organism part

text: Mus musculus

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

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