characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF COPENHAGEN

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.1

characteristic: binning software

text: Chicken digestive system

characteristic: broad-scale environmental context

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a binned metagenome assembled from the metagenomic run ERR4993382 of study ERP125074.

characteristic: description

text: Caecum

text: Spain

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: chicken gut metagenome

text: Digestive tube

text: chicken gut metagenome

text: uncultured Firmicutes bacterium

text: uncultured Firmicutes bacterium

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__;o__;f__;g__;s__

text: multi-marker approach

text: Binned metagenome: ERR4993382_bin.8_MetaWRAP_v1.1_bin

characteristic: title


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF COPENHAGEN

text: public

tag: Namespace:UNIVERSITY OF COPENHAGEN

text: not provided

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: MetaWRAP v1.1

text: Chicken digestive system

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a binned metagenome assembled from the metagenomic run ERR4968712 of study ERP125074.

text: Caecum

characteristic: environmental medium

text: Spain

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: chicken gut metagenome

text: Digestive tube

characteristic: local environmental context

text: chicken gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: organism

text: Metagenomic raw reads, assemblies, and bins derived from HoloFood chicken caecum samples. Samples selected for this batch were deeply sequenced, and were randomised among trials (feed), age, and breed to overcome batch effect. The samples in this project contributed to the chicken caecum MAG catalogue (project: PRJEB55374 [ERP140263]).

characteristic: project name

characteristic: sample derived from

text: uncultured Clostridia bacterium

characteristic: sequencing method

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Binned metagenome: ERR4968712_bin.24_MetaWRAP_v1.1_bin


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF COPENHAGEN

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.1

characteristic: binning software

text: Chicken digestive system

characteristic: broad-scale environmental context

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a binned metagenome assembled from the metagenomic run ERR4968596 of study ERP125074.

characteristic: description

text: Caecum

text: Spain

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: chicken gut metagenome

text: Digestive tube

text: chicken gut metagenome

text: uncultured Oscillospiraceae bacterium

text: uncultured Oscillospiraceae bacterium

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Eubacteriales;f__Oscillospiraceae;g__;s__

text: multi-marker approach

text: Binned metagenome: ERR4968596_bin.26_MetaWRAP_v1.1_bin

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF COPENHAGEN

text: public

text: not provided

text: MetaWRAP v1.1

text: Chicken digestive system

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a binned metagenome assembled from the metagenomic run ERR4968595 of study ERP125074.

text: Caecum

characteristic: environmental medium

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: chicken gut metagenome

text: Digestive tube

characteristic: local environmental context

text: chicken gut metagenome

characteristic: metagenomic source

text: Candidatus Limadaptatus stercoravium

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: Candidatus Limadaptatus stercoravium

characteristic: sequencing method

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Eubacteriales;f__;g__Candidatus Limadaptatus;s__Candidatus Limadaptatus stercoravium

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Binned metagenome: ERR4968595_bin.17_MetaWRAP_v1.1_bin


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF COPENHAGEN

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.1

characteristic: binning software

text: Chicken digestive system

characteristic: broad-scale environmental context

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Caecum

text: Spain

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: chicken gut metagenome

text: Digestive tube

text: chicken gut metagenome

text: Bacteroides congonensis

text: Bacteroides congonensis

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Bacteroides;s__Bacteroides congonensis

text: multi-marker approach

text: Binned metagenome: ERR4968595_bin.14_MetaWRAP_v1.1_bin

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF COPENHAGEN

text: public

text: not provided

text: MetaWRAP v1.1

text: Chicken digestive system

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a binned metagenome assembled from the metagenomic run ERR7167029 of study ERP125074.

text: Caecum

characteristic: environmental medium

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: chicken gut metagenome

text: Digestive tube

characteristic: local environmental context

text: chicken gut metagenome

characteristic: metagenomic source

text: uncultured Bacilli bacterium

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: uncultured Bacilli bacterium

characteristic: sequencing method

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Bacilli;o__;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Binned metagenome: ERR7167029_bin.17_MetaWRAP_v1.1_bin


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF COPENHAGEN

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.1

characteristic: binning software

text: Chicken digestive system

characteristic: broad-scale environmental context

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a binned metagenome assembled from the metagenomic run ERR4968604 of study ERP125074.

characteristic: description

text: Caecum

text: Spain

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: chicken gut metagenome

text: Digestive tube

text: chicken gut metagenome

text: uncultured Eisenbergiella sp.

text: uncultured Eisenbergiella sp.

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Eubacteriales;f__Lachnospiraceae;g__Eisenbergiella;s__

text: multi-marker approach

text: Binned metagenome: ERR4968604_bin.16_MetaWRAP_v1.1_bin

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF COPENHAGEN

text: public

text: not provided

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: MetaWRAP v1.1

text: Chicken digestive system

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a binned metagenome assembled from the metagenomic run ERR4968687 of study ERP125074.

text: Caecum

characteristic: environmental medium

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: chicken gut metagenome

text: Digestive tube

characteristic: local environmental context

text: chicken gut metagenome

characteristic: metagenomic source

text: uncultured Oscillospiraceae bacterium

characteristic: organism

text: Metagenomic raw reads, assemblies, and bins derived from HoloFood chicken caecum samples. Samples selected for this batch were deeply sequenced, and were randomised among trials (feed), age, and breed to overcome batch effect. The samples in this project contributed to the chicken caecum MAG catalogue (project: PRJEB55374 [ERP140263]).

characteristic: project name

characteristic: sample derived from

text: uncultured Oscillospiraceae bacterium

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Binned metagenome: ERR4968687_bin.71_MetaWRAP_v1.1_bin


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF COPENHAGEN

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.1

characteristic: binning software

text: Chicken digestive system

characteristic: broad-scale environmental context

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a binned metagenome assembled from the metagenomic run ERR4968598 of study ERP125074.

characteristic: description

text: Caecum

text: Spain

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: chicken gut metagenome

text: Digestive tube

text: chicken gut metagenome

text: uncultured Anaeromassilibacillus sp.

text: uncultured Anaeromassilibacillus sp.

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Eubacteriales;f__Oscillospiraceae;g__Anaeromassilibacillus;s__

text: multi-marker approach

text: Binned metagenome: ERR4968598_bin.12_MetaWRAP_v1.1_bin

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF COPENHAGEN

text: public

text: not provided

text: MetaWRAP v1.1

text: Chicken digestive system

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a binned metagenome assembled from the metagenomic run ERR4968565 of study ERP125074.

text: Caecum

characteristic: environmental medium

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: chicken gut metagenome

text: Digestive tube

characteristic: local environmental context

text: chicken gut metagenome

characteristic: metagenomic source

text: Akkermansia muciniphila

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: Akkermansia muciniphila

characteristic: sequencing method

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Verrucomicrobia;c__Verrucomicrobiae;o__Verrucomicrobiales;f__Akkermansiaceae;g__Akkermansia;s__Akkermansia muciniphila

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Binned metagenome: ERR4968565_bin.14_MetaWRAP_v1.1_bin


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF COPENHAGEN

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.1

characteristic: binning software

text: Chicken digestive system

characteristic: broad-scale environmental context

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a binned metagenome assembled from the metagenomic run ERR4968554 of study ERP125074.

characteristic: description

text: Caecum

text: Spain

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: chicken gut metagenome

text: Digestive tube

text: chicken gut metagenome

text: uncultured Clostridium sp.

text: uncultured Clostridium sp.

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Eubacteriales;f__Clostridiaceae;g__Clostridium;s__

text: multi-marker approach

text: Binned metagenome: ERR4968554_bin.11_MetaWRAP_v1.1_bin

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF COPENHAGEN

text: public

text: not provided

text: MetaWRAP v1.1

text: Chicken digestive system

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a binned metagenome assembled from the metagenomic run ERR4968582 of study ERP125074.

text: Caecum

characteristic: environmental medium

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: chicken gut metagenome

text: Digestive tube

characteristic: local environmental context

text: chicken gut metagenome

characteristic: metagenomic source

text: uncultured Desulfovibrio sp.

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: uncultured Desulfovibrio sp.

characteristic: sequencing method

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Deltaproteobacteria;o__Desulfovibrionales;f__Desulfovibrionaceae;g__Desulfovibrio;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Binned metagenome: ERR4968582_bin.19_MetaWRAP_v1.1_bin


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF COPENHAGEN

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.1

characteristic: binning software

text: Chicken digestive system

characteristic: broad-scale environmental context

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a binned metagenome assembled from the metagenomic run ERR4993381 of study ERP125074.

characteristic: description

text: Caecum

text: Spain

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: chicken gut metagenome

text: Digestive tube

text: chicken gut metagenome

text: Bacteroides fragilis

text: Bacteroides fragilis

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Bacteroides;s__Bacteroides fragilis

text: multi-marker approach

text: Binned metagenome: ERR4993381_bin.9_MetaWRAP_v1.1_bin

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF COPENHAGEN

text: public

text: not provided

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: MetaWRAP v1.1

text: Chicken digestive system

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Caecum

characteristic: environmental medium

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: chicken gut metagenome

text: Digestive tube

characteristic: local environmental context

text: chicken gut metagenome

characteristic: metagenomic source

text: uncultured Eisenbergiella sp.

characteristic: organism

text: Metagenomic raw reads, assemblies, and bins derived from HoloFood chicken caecum samples. Samples selected for this batch were deeply sequenced, and were randomised among trials (feed), age, and breed to overcome batch effect. The samples in this project contributed to the chicken caecum MAG catalogue (project: PRJEB55374 [ERP140263]).

characteristic: project name

characteristic: sample derived from

text: uncultured Eisenbergiella sp.

characteristic: sequencing method

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Eubacteriales;f__Lachnospiraceae;g__Eisenbergiella;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Binned metagenome: ERR4968598_bin.39_MetaWRAP_v1.1_bin


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF COPENHAGEN

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.1

characteristic: binning software

text: Chicken digestive system

characteristic: broad-scale environmental context

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a binned metagenome assembled from the metagenomic run ERR4992208 of study ERP125074.

characteristic: description

text: Caecum

text: Spain

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: chicken gut metagenome

text: Digestive tube

text: chicken gut metagenome

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Eubacteriales;f__Lachnospiraceae;g__Eisenbergiella;s__Candidatus Eisenbergiella merdigallinarum

text: multi-marker approach

text: Binned metagenome: ERR4992208_bin.25_MetaWRAP_v1.1_bin

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF COPENHAGEN

text: public

text: not provided

text: MetaWRAP v1.1

text: Chicken digestive system

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a binned metagenome assembled from the metagenomic run ERR4968704 of study ERP125074.

text: Caecum

characteristic: environmental medium

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: chicken gut metagenome

text: Digestive tube

characteristic: local environmental context

text: chicken gut metagenome

characteristic: metagenomic source

text: uncultured Blautia sp.

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: uncultured Blautia sp.

characteristic: sequencing method

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Eubacteriales;f__Lachnospiraceae;g__Blautia;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Binned metagenome: ERR4968704_bin.50_MetaWRAP_v1.1_bin


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF COPENHAGEN

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.1

characteristic: binning software

text: Chicken digestive system

characteristic: broad-scale environmental context

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Caecum

text: Spain

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: chicken gut metagenome

text: Digestive tube

text: chicken gut metagenome

text: uncultured Firmicutes bacterium

text: uncultured Firmicutes bacterium

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__;o__;f__;g__;s__

text: multi-marker approach

text: Binned metagenome: ERR4968582_bin.20_MetaWRAP_v1.1_bin

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF COPENHAGEN

text: public

text: not provided

text: MetaWRAP v1.1

text: Chicken digestive system

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a binned metagenome assembled from the metagenomic run ERR4968705 of study ERP125074.

text: Caecum

characteristic: environmental medium

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: chicken gut metagenome

text: Digestive tube

characteristic: local environmental context

text: chicken gut metagenome

characteristic: metagenomic source

characteristic: organism

characteristic: project name

characteristic: sample derived from

characteristic: sequencing method

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__;c__;o__;f__;g__Candidatus Galligastranaerophilus;s__Candidatus Galligastranaerophilus faecipullorum

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Binned metagenome: ERR4968705_bin.104_MetaWRAP_v1.1_bin


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF COPENHAGEN

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.1

characteristic: binning software

text: Chicken digestive system

characteristic: broad-scale environmental context

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a binned metagenome assembled from the metagenomic run ERR4968711 of study ERP125074.

characteristic: description

text: Caecum

text: Spain

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: chicken gut metagenome

text: Digestive tube

text: chicken gut metagenome

text: Anaerotruncus colihominis

text: Anaerotruncus colihominis

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Eubacteriales;f__Oscillospiraceae;g__Anaerotruncus;s__Anaerotruncus colihominis

text: multi-marker approach

text: Binned metagenome: ERR4968711_bin.8_MetaWRAP_v1.1_bin

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF COPENHAGEN

text: public

text: not provided

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: MetaWRAP v1.1

text: Chicken digestive system

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a binned metagenome assembled from the metagenomic run ERR4968558 of study ERP125074.

text: Caecum

characteristic: environmental medium

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: chicken gut metagenome

text: Digestive tube

characteristic: local environmental context

text: chicken gut metagenome

characteristic: metagenomic source

text: Ligilactobacillus aviarius

characteristic: organism

text: Metagenomic raw reads, assemblies, and bins derived from HoloFood chicken caecum samples. Samples selected for this batch were deeply sequenced, and were randomised among trials (feed), age, and breed to overcome batch effect. The samples in this project contributed to the chicken caecum MAG catalogue (project: PRJEB55374 [ERP140263]).

characteristic: project name

characteristic: sample derived from

text: Ligilactobacillus aviarius

characteristic: sequencing method

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Ligilactobacillus;s__Ligilactobacillus aviarius

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Binned metagenome: ERR4968558_bin.13_MetaWRAP_v1.1_bin


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF COPENHAGEN

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.1

characteristic: binning software

text: Chicken digestive system

characteristic: broad-scale environmental context

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a binned metagenome assembled from the metagenomic run ERR7167043 of study ERP125074.

characteristic: description

text: Caecum

text: Spain

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: chicken gut metagenome

text: Digestive tube

text: chicken gut metagenome

text: uncultured Lachnoclostridium sp.

text: uncultured Lachnoclostridium sp.

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Eubacteriales;f__Lachnospiraceae;g__Lachnoclostridium;s__

text: multi-marker approach

text: Binned metagenome: ERR7167043_bin.1_MetaWRAP_v1.1_bin

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF COPENHAGEN

text: public

text: not provided

text: MetaWRAP v1.1

text: Chicken digestive system

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a binned metagenome assembled from the metagenomic run ERR4968585 of study ERP125074.

text: Caecum

characteristic: environmental medium

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: chicken gut metagenome

text: Digestive tube

characteristic: local environmental context

text: chicken gut metagenome

characteristic: metagenomic source

text: uncultured Eubacteriales bacterium

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: uncultured Eubacteriales bacterium

characteristic: sequencing method

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Eubacteriales;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Binned metagenome: ERR4968585_bin.15_MetaWRAP_v1.1_bin


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF COPENHAGEN

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.1

characteristic: binning software

text: Chicken digestive system

characteristic: broad-scale environmental context

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Caecum

text: Spain

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: chicken gut metagenome

text: Digestive tube

text: chicken gut metagenome

text: uncultured Prevotella sp.

text: uncultured Prevotella sp.

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Prevotellaceae;g__Prevotella;s__

text: multi-marker approach

text: Binned metagenome: ERR4968558_bin.9_MetaWRAP_v1.1_bin

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF COPENHAGEN

text: public

text: not provided

text: MetaWRAP v1.1

text: Chicken digestive system

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a binned metagenome assembled from the metagenomic run ERR4969151 of study ERP125074.

text: Caecum

characteristic: environmental medium

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: chicken gut metagenome

text: Digestive tube

characteristic: local environmental context

text: chicken gut metagenome

characteristic: metagenomic source

text: uncultured Christensenellaceae bacterium

characteristic: organism

characteristic: project name

characteristic: sample derived from

characteristic: sequencing method

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Eubacteriales;f__Christensenellaceae;g__Candidatus Onthoplasma;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Binned metagenome: ERR4969151_bin.98_MetaWRAP_v1.1_bin


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF COPENHAGEN

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not provided

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: MetaWRAP v1.1

characteristic: binning software

text: Chicken digestive system

characteristic: broad-scale environmental context

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Caecum

text: Spain

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: chicken gut metagenome

text: Digestive tube

text: chicken gut metagenome

text: uncultured Oscillospiraceae bacterium

text: uncultured Oscillospiraceae bacterium

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Eubacteriales;f__Oscillospiraceae;g__;s__

text: multi-marker approach

text: Binned metagenome: ERR4969151_bin.49_MetaWRAP_v1.1_bin

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF COPENHAGEN

text: public

text: not provided

text: MetaWRAP v1.1

text: Chicken digestive system

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a binned metagenome assembled from the metagenomic run ERR4968671 of study ERP125074.

text: Caecum

characteristic: environmental medium

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: chicken gut metagenome

text: Digestive tube

characteristic: local environmental context

text: chicken gut metagenome

characteristic: metagenomic source

text: uncultured Bacilli bacterium

characteristic: organism

text: Metagenomic raw reads, assemblies, and bins derived from HoloFood chicken caecum samples. Samples selected for this batch were deeply sequenced, and were randomised among trials (feed), age, and breed to overcome batch effect. The samples in this project contributed to the chicken caecum MAG catalogue (project: PRJEB55374 [ERP140263]).

characteristic: project name

characteristic: sample derived from

text: uncultured Bacilli bacterium

characteristic: sequencing method

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Bacilli;o__;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Binned metagenome: ERR4968671_bin.1_MetaWRAP_v1.1_bin


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF COPENHAGEN

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.1

characteristic: binning software

text: Chicken digestive system

characteristic: broad-scale environmental context

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a binned metagenome assembled from the metagenomic run ERR4968673 of study ERP125074.

characteristic: description

text: Caecum

text: Spain

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: chicken gut metagenome

text: Digestive tube

text: chicken gut metagenome

text: Sellimonas intestinalis

text: Sellimonas intestinalis

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Eubacteriales;f__Lachnospiraceae;g__Sellimonas;s__Sellimonas intestinalis

text: multi-marker approach

text: Binned metagenome: ERR4968673_bin.14_MetaWRAP_v1.1_bin

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF COPENHAGEN

text: public

text: not provided

text: MetaWRAP v1.1

text: Chicken digestive system

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a binned metagenome assembled from the metagenomic run ERR4968590 of study ERP125074.

text: Caecum

characteristic: environmental medium

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: chicken gut metagenome

text: Digestive tube

characteristic: local environmental context

text: chicken gut metagenome

characteristic: metagenomic source

text: uncultured Oscillospiraceae bacterium

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: uncultured Oscillospiraceae bacterium

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Binned metagenome: ERR4968590_bin.8_MetaWRAP_v1.1_bin


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF COPENHAGEN

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.1

characteristic: binning software

text: Chicken digestive system

characteristic: broad-scale environmental context

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a binned metagenome assembled from the metagenomic run ERR4968602 of study ERP125074.

characteristic: description

text: Caecum

text: Spain

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: chicken gut metagenome

text: Digestive tube

text: chicken gut metagenome

text: uncultured Lachnoclostridium sp.

text: uncultured Lachnoclostridium sp.

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Eubacteriales;f__Lachnospiraceae;g__Lachnoclostridium;s__

text: multi-marker approach

text: Binned metagenome: ERR4968602_bin.78_MetaWRAP_v1.1_bin

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF COPENHAGEN

text: public

text: not provided

text: MetaWRAP v1.1

text: Chicken digestive system

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a binned metagenome assembled from the metagenomic run ERR4968594 of study ERP125074.

text: Caecum

characteristic: environmental medium

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: chicken gut metagenome

text: Digestive tube

characteristic: local environmental context

text: chicken gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: uncultured Clostridia bacterium

characteristic: sequencing method

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Binned metagenome: ERR4968594_bin.12_MetaWRAP_v1.1_bin


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF COPENHAGEN

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not provided

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: MetaWRAP v1.1

characteristic: binning software

text: Chicken digestive system

characteristic: broad-scale environmental context

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a binned metagenome assembled from the metagenomic run ERR7167043 of study ERP125074.

characteristic: description

text: Caecum

text: Spain

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: chicken gut metagenome

text: Digestive tube

text: chicken gut metagenome

text: uncultured Flavonifractor sp.

text: uncultured Flavonifractor sp.

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Eubacteriales;f__Oscillospiraceae;g__Flavonifractor;s__

text: multi-marker approach

text: Binned metagenome: ERR7167043_bin.11_MetaWRAP_v1.1_bin

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF COPENHAGEN

text: public

text: not provided

text: MetaWRAP v1.1

text: Chicken digestive system

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a binned metagenome assembled from the metagenomic run ERR4968569 of study ERP125074.

text: Caecum

characteristic: environmental medium

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: chicken gut metagenome

text: Digestive tube

characteristic: local environmental context

text: chicken gut metagenome

characteristic: metagenomic source

text: uncultured Acetobacter sp.

characteristic: organism

text: Metagenomic raw reads, assemblies, and bins derived from HoloFood chicken caecum samples. Samples selected for this batch were deeply sequenced, and were randomised among trials (feed), age, and breed to overcome batch effect. The samples in this project contributed to the chicken caecum MAG catalogue (project: PRJEB55374 [ERP140263]).

characteristic: project name

characteristic: sample derived from

text: uncultured Acetobacter sp.

characteristic: sequencing method

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhodospirillales;f__Acetobacteraceae;g__Acetobacter;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Binned metagenome: ERR4968569_bin.4_MetaWRAP_v1.1_bin


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF COPENHAGEN

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.1

characteristic: binning software

text: Chicken digestive system

characteristic: broad-scale environmental context

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a binned metagenome assembled from the metagenomic run ERR4968608 of study ERP125074.

characteristic: description

text: Caecum

text: Spain

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: chicken gut metagenome

text: Digestive tube

text: chicken gut metagenome

text: uncultured Eubacteriales bacterium

text: uncultured Eubacteriales bacterium

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Eubacteriales;f__;g__;s__

text: multi-marker approach

text: Binned metagenome: ERR4968608_bin.16_MetaWRAP_v1.1_bin

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF COPENHAGEN

text: public

text: not provided

text: MetaWRAP v1.1

text: Chicken digestive system

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a binned metagenome assembled from the metagenomic run ERR4968571 of study ERP125074.

text: Caecum

characteristic: environmental medium

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: chicken gut metagenome

text: Digestive tube

characteristic: local environmental context

text: chicken gut metagenome

characteristic: metagenomic source

text: Candidatus Mediterraneibacter merdavium

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: Candidatus Mediterraneibacter merdavium

characteristic: sequencing method

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Eubacteriales;f__Lachnospiraceae;g__Mediterraneibacter;s__Candidatus Mediterraneibacter merdavium

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Binned metagenome: ERR4968571_bin.23_MetaWRAP_v1.1_bin


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF COPENHAGEN

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.1

characteristic: binning software

text: Chicken digestive system

characteristic: broad-scale environmental context

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a binned metagenome assembled from the metagenomic run ERR4993378 of study ERP125074.

characteristic: description

text: Caecum

text: Spain

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: chicken gut metagenome

text: Digestive tube

text: chicken gut metagenome

text: uncultured Lachnoclostridium sp.

text: uncultured Lachnoclostridium sp.

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Eubacteriales;f__Lachnospiraceae;g__Lachnoclostridium;s__

text: multi-marker approach

text: Binned metagenome: ERR4993378_bin.35_MetaWRAP_v1.1_bin

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF COPENHAGEN

text: public

text: not provided

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: MetaWRAP v1.1

text: Chicken digestive system

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Caecum

characteristic: environmental medium

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: chicken gut metagenome

text: Digestive tube

characteristic: local environmental context

text: chicken gut metagenome

characteristic: metagenomic source

characteristic: organism

characteristic: project name

characteristic: sample derived from

characteristic: sequencing method

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Eubacteriales;f__Lachnospiraceae;g__Lachnoclostridium;s__Candidatus Lachnoclostridium stercoripullorum

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Binned metagenome: ERR4968608_bin.41_MetaWRAP_v1.1_bin


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF COPENHAGEN

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.1

characteristic: binning software

text: Chicken digestive system

characteristic: broad-scale environmental context

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a binned metagenome assembled from the metagenomic run ERR4968599 of study ERP125074.

characteristic: description

text: Caecum

text: Spain

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: chicken gut metagenome

text: Digestive tube

text: chicken gut metagenome

text: uncultured Eubacteriales bacterium

text: uncultured Eubacteriales bacterium

text: multi-marker approach

text: Binned metagenome: ERR4968599_bin.44_MetaWRAP_v1.1_bin

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF COPENHAGEN

text: public

text: not provided

text: MetaWRAP v1.1

text: Chicken digestive system

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a binned metagenome assembled from the metagenomic run ERR4968711 of study ERP125074.

text: Caecum

characteristic: environmental medium

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: chicken gut metagenome

text: Digestive tube

characteristic: local environmental context

text: chicken gut metagenome

characteristic: metagenomic source

text: Candidatus Blautia stercorigallinarum

characteristic: organism

text: Metagenomic raw reads, assemblies, and bins derived from HoloFood chicken caecum samples. Samples selected for this batch were deeply sequenced, and were randomised among trials (feed), age, and breed to overcome batch effect. The samples in this project contributed to the chicken caecum MAG catalogue (project: PRJEB55374 [ERP140263]).

characteristic: project name

characteristic: sample derived from

text: Candidatus Blautia stercorigallinarum

characteristic: sequencing method

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Eubacteriales;f__Lachnospiraceae;g__Blautia;s__Candidatus Blautia stercorigallinarum

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Binned metagenome: ERR4968711_bin.20_MetaWRAP_v1.1_bin


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF COPENHAGEN

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.1

characteristic: binning software

text: Chicken digestive system

characteristic: broad-scale environmental context

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a binned metagenome assembled from the metagenomic run ERR4968579 of study ERP125074.

characteristic: description

text: Caecum

text: Spain

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: chicken gut metagenome

text: Digestive tube

text: chicken gut metagenome

text: uncultured Firmicutes bacterium

text: uncultured Firmicutes bacterium

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__;o__;f__;g__;s__

text: multi-marker approach

text: Binned metagenome: ERR4968579_bin.5_MetaWRAP_v1.1_bin

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF COPENHAGEN

text: public

text: not provided

text: MetaWRAP v1.1

text: Chicken digestive system

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a binned metagenome assembled from the metagenomic run ERR4968585 of study ERP125074.

text: Caecum

characteristic: environmental medium

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: chicken gut metagenome

text: Digestive tube

characteristic: local environmental context

text: chicken gut metagenome

characteristic: metagenomic source

text: uncultured Prevotella sp.

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: uncultured Prevotella sp.

characteristic: sequencing method

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Prevotellaceae;g__Prevotella;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Binned metagenome: ERR4968585_bin.28_MetaWRAP_v1.1_bin


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF COPENHAGEN

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.1

characteristic: binning software

text: Chicken digestive system

characteristic: broad-scale environmental context

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

characteristic: description

text: Caecum

text: Spain

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: chicken gut metagenome

text: Digestive tube

text: chicken gut metagenome

text: Candidatus Heritagella intestinalis

text: Candidatus Heritagella intestinalis

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__;f__;g__Candidatus Heritagella;s__Candidatus Heritagella intestinalis

text: multi-marker approach

text: Binned metagenome: ERR4968599_bin.4_MetaWRAP_v1.1_bin

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF COPENHAGEN

text: public

text: not provided

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: MetaWRAP v1.1

text: Chicken digestive system

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a binned metagenome assembled from the metagenomic run ERR4968605 of study ERP125074.

text: Caecum

characteristic: environmental medium

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: chicken gut metagenome

text: Digestive tube

characteristic: local environmental context

text: chicken gut metagenome

characteristic: metagenomic source

text: uncultured Eubacteriales bacterium

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: uncultured Eubacteriales bacterium

characteristic: sequencing method

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Eubacteriales;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Binned metagenome: ERR4968605_bin.5_MetaWRAP_v1.1_bin


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF COPENHAGEN

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.1

characteristic: binning software

text: Chicken digestive system

characteristic: broad-scale environmental context

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a binned metagenome assembled from the metagenomic run ERR7167060 of study ERP125074.

characteristic: description

text: Caecum

text: Spain

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: chicken gut metagenome

text: Digestive tube

text: chicken gut metagenome

text: Anaerotignum lactatifermentans

text: Anaerotignum lactatifermentans

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Eubacteriales;f__Lachnospiraceae;g__Anaerotignum;s__Anaerotignum lactatifermentans

text: multi-marker approach

text: Binned metagenome: ERR7167060_bin.4_MetaWRAP_v1.1_bin

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF COPENHAGEN

text: public

text: not provided

text: MetaWRAP v1.1

text: Chicken digestive system

text: EMG broker account, EMBL-EBI

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a binned metagenome assembled from the metagenomic run ERR4968678 of study ERP125074.

text: Caecum

characteristic: environmental medium

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: chicken gut metagenome

text: Digestive tube

characteristic: local environmental context

text: chicken gut metagenome

characteristic: metagenomic source

text: uncultured bacterium

characteristic: organism

text: Metagenomic raw reads, assemblies, and bins derived from HoloFood chicken caecum samples. Samples selected for this batch were deeply sequenced, and were randomised among trials (feed), age, and breed to overcome batch effect. The samples in this project contributed to the chicken caecum MAG catalogue (project: PRJEB55374 [ERP140263]).

characteristic: project name

characteristic: sample derived from

text: uncultured bacterium

characteristic: sequencing method

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__;c__;o__;f__;g__Candidatus Galligastranaerophilus;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Binned metagenome: ERR4968678_bin.34_MetaWRAP_v1.1_bin


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF COPENHAGEN

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.1

characteristic: binning software

text: Chicken digestive system

characteristic: broad-scale environmental context

text: EMG broker account, EMBL-EBI

characteristic: broker name

text: CheckM

text: This sample represents a binned metagenome assembled from the metagenomic run ERR4968557 of study ERP125074.

characteristic: description

text: Caecum

text: Spain

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: chicken gut metagenome

text: Digestive tube

text: chicken gut metagenome

text: The taxonomy of this binned metagenome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__;c__;o__;f__;g__Candidatus Galligastranaerophilus;s__Candidatus Galligastranaerophilus faecipullorum

text: multi-marker approach

text: Binned metagenome: ERR4968557_bin.13_MetaWRAP_v1.1_bin

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UT SOUTHWESTERN

text: public

tag: Namespace:UT SOUTHWESTERN

text: human

characteristic: common name

text: Targeted sequencing of IDH1 and IDH2 genes was performed using bone marrow specimens from matched diagnosis and relapsed AML patients with IDH baseline mutation before or after IDHmut inhibitor treatment. gDNA was extracted from the bone marrow clot sections using the QuickExtract FFPE DNA extraction kit (Epicenter Biotechnologies) following the manufacturer's instructions. The exon sequences of IDH1 and IDH2 genes were PCR amplified. The DNA fragments for each exon were purified with QIAquick spin columns (Qiagen), mixed at equal molarity, and fragmented using Covaris acoustic shearing following the manufacturer�s instructions. Sonicated DNA was processed for library generation using NEBNext Ultra II DNA Kits (New England Biolabs) following the manufacturer�s protocol. Libraries with different index sequences were quantified, pooled, and sequenced on an Illumina Nextseq500 system using the 75bp high output sequencing kit.

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Targeted sequencing of IDH1 and IDH2 genes in AML samples


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Aberystwyth University

characteristic: INSDC center name

characteristic: INSDC first public

text: public

tag: Namespace:Aberystwyth University

text: Melanie Hay, Aliyah Debbonnaire

text: Cryoconite metagenome

text: Yes

text: Svalbard

text: Austre Br�ggerbreen

characteristic: geographic location (region and locality)

text: Cryoconite

text: 78d 53' 44 N,11d 49' 54 E

text: glacier metagenome

text: glacier metagenome

text: Cryoconite AB


text: Aberystwyth University

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Melanie Hay, Aliyah Debbonnaire

text: Cryoconite metagenome

text: Yes

text: Svalbard

text: Vestre Lov�nbreen

text: Cryoconite

text: 78d 54' 14 N,11d 56' 27 E

text: glacier metagenome

text: glacier metagenome

text: Cryoconite VL


text: Aberystwyth University

text: public

text: Melanie Hay, Aliyah Debbonnaire

text: Cryoconite metagenome

text: Yes

text: Svalbard

text: Vestre Br�ggerbreen

text: Cryoconite

text: 78d 54' 40 N,11d 43' 60 E

text: glacier metagenome

text: glacier metagenome

text: Cryoconite VB


text: Aberystwyth University

text: public

text: Melanie Hay, Aliyah Debbonnaire

text: Cryoconite metagenome

text: Yes

text: Svalbard

text: Midtre Lov�nbreen

text: Cryoconite

text: 78d 53' 17 N,12d 02' 49 E

text: glacier metagenome

text: glacier metagenome

text: Cryoconite ML


text: Aberystwyth University

text: public

text: Arwyn Edwards

text: Cryoconite metagenome

text: Yes

text: Svalbard

text: Midtre Lov�nbreen

text: Cryoconite

text: 78d 53' 32 N,12d 03' 18 E

text: glacier metagenome

text: glacier metagenome

text: Cryoconite ML


text: Aberystwyth University

text: public

text: Nora Els

text: Cryoconite metagenome

text: Yes

text: Svalbard

text: Vestre Br�ggerbreen

text: Cryoconite

text: 78d 54' 41 N,11d 43' 58 E

text: glacier metagenome

text: glacier metagenome

text: Cryoconite VB


text: Aberystwyth University

text: public

text: Melanie Hay

text: Soil metagenome

text: Yes

text: Svalbard

text: Midtre Lov�nbreen Forefield

text: Soil

text: 78d 53' 54 N,12d 03' 58 E

text: soil metagenome

text: soil metagenome

text: glacier forefield soil ML


text: Aberystwyth University

text: public

text: Melanie Hay

text: Soil metagenome

text: Yes

text: Svalbard

text: Midtre Lov�nbreen Forefield

text: Soil

text: 78d 53' 57 N,12d 04' 0 E

text: soil metagenome

text: soil metagenome

text: glacier forefield soil ML


text: Aberystwyth University

text: public

text: Melanie Hay

text: Soil metagenome

text: Yes

text: Svalbard

text: Midtre Lov�nbreen Forefield

text: Soil

text: 78d 53' 49 N,12d 04' 05 E

text: soil metagenome

text: soil metagenome

text: glacier forefield soil ML


text: Aberystwyth University

text: public

text: Melanie Hay

text: Soil metagenome

text: Yes

text: Svalbard

text: Midtre Lov�nbreen Forefield

text: Soil

text: 78d 53' 57 N,12d 04' 14 E

text: soil metagenome

text: soil metagenome

text: glacier forefield soil ML


text: Aberystwyth University

text: public

text: Melanie Hay

text: Soil metagenome

text: Yes

text: Svalbard

text: Midtre Lov�nbreen Forefield

text: Soil

text: 78d 53' 44 N,12d 04' 05 E

text: soil metagenome

text: soil metagenome

text: glacier forefield soil ML


text: Aberystwyth University

text: public

text: Melanie Hay

text: Soil metagenome

text: Yes

text: Svalbard

text: Midtre Lov�nbreen Forefield

text: Soil

text: 78d 53' 53 N,12d 04' 17 E

text: soil metagenome

text: soil metagenome

text: glacier forefield soil ML


text: Aberystwyth University

text: public

text: Melanie Hay

text: Seawater metagenome

text: Yes

text: Svalbard

text: Kongsfjorden Fjord

text: Seawater

text: 78d 55' 27 N,12d 03' 59 E

text: seawater metagenome

text: seawater metagenome

text: Seawater (shallow)


text: Aberystwyth University

text: public

text: Melanie Hay

text: Seawater metagenome

text: Yes

text: Svalbard

text: Kongsfjorden Fjord

text: Seawater

text: 78d 55' 24 N,12d 04' 10 E

text: seawater metagenome

text: seawater metagenome

text: Seawater (shallow)


text: Aberystwyth University

text: public

text: Melanie Hay

text: Seawater metagenome

text: Yes

text: Svalbard

text: Kongsfjorden Fjord

text: Seawater

text: 78d 55' 22 N,12d 04' 53 E

text: seawater metagenome

text: seawater metagenome

text: Seawater (shallow)


text: Plasmodium falciparum

characteristic: ArrayExpress-SPECIES

text: SC

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum


text: public

tag: Namespace:NATIONAL INSTITUTE OF HEALTH DR. RICARDO JORGE

characteristic: TITLE

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: NATIONAL INSTITUTE OF HEALTH DR. RICARDO JORGE

text: public

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


E-MTAB-12532:12h rep1

text: Walter and Eliza Hall Institute

text: public

text: E-MTAB-12532:12h rep1

tag: Namespace:Walter and Eliza Hall Institute

text: ArrayExpress

characteristic: broker name

text: thale cress

text: Protocols: Col-0 seeds were sown and collected at the 12 h, 24 h and 48 h time-points, in three independent replicates (batches) of the experiment. Col-0 seeds were sown on MS media plates (containing 3 % sucrose). Seeds were sterilised and stratified for 48 h of cold (4°C) dark stratification before being transferred to continuous light (at 22°C), then collected after 12 h, 24 h and 48 h in the light. Dissociation of Arabidopsis thaliana embryos into single cells: seeds were sandwiched between two glass slides and embryos were released mechanically from seed coats by pressing two slides. Ruptured seed coats and embryos were collected into microcentrifuge tubes and separated from each other using Percoll gradient. In brief, sample was resuspended in MC buffer (10mM potassium phospate pH7.0, 50mM NaCl, 0.1M sucrose) and loaded onto a 1 ml 60% Percoll cushion. Sample was centrifuged at 800 g for 5 min and fraction of embryos (at the bottom of the tube) was collected and re-suspended in 0.6 ml MC buffer. A second Percoll gradient was repeated to obtain pellet of embryos without any seed coats. Enriched embryos samples were cut with razor blades and digested in protoplasting buffer (2 % w/v Cellulase, 3 % w/v Macerozyme, 0.4M D-mannitol, 20~mM MES, 20~mM KCl in milli-Q water and adjust the pH to 5.7 with 1~M Tris/HCL pH7.5, 0.1 % w/v BSA, 10~mM CaCl2, and 5~mM β-mercaptoethanol). After 3 hour of digestion, protoplasts were filtered through a 70 μm cell strainer, then 40 μm cell strainer to remove debris and centrifuged at 500 g for 5 min. Supernatant was removed and pellet was washed with 2 ml protoplasting buffer without enzymes and mercaptoethanol and centrifuged at 500 g for 5 min. Pellet was resuspended in 50 μl protoplasting buffer without enzymes and mercaptoethanol. Protoplasts were counted using hemocytometer and adjusted to final concentration of 800-1200 protoplasts per μl. QIAgen Plant RNEasy kit 10x Genomics 3’ end droplet-based single-cell RNA-seq (kit v2)

characteristic: description

text: seed germination stage

characteristic: developmental stage

characteristic: ecotype

text: wild type genotype

characteristic: genotype

text: 12h germinating in the light

characteristic: growth condition

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: embryo

characteristic: organism part

characteristic: replicate

text: Arabidopsis thaliana

text: 12h rep1


E-MTAB-12532:12h rep2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Walter and Eliza Hall Institute

characteristic: INSDC center name

text: public

text: E-MTAB-12532:12h rep2

text: ArrayExpress

text: thale cress

characteristic: common name

text: seed germination stage

text: wild type genotype

text: 12h germinating in the light

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis thaliana

text: embryo

text: Arabidopsis thaliana

text: 12h rep2

characteristic: title


E-MTAB-12532:24h rep1

text: Walter and Eliza Hall Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12532:24h rep1

characteristic: Submitter Id

text: ArrayExpress

text: thale cress

text: seed germination stage

text: wild type genotype

text: 24h germinating in the light

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: embryo

text: Arabidopsis thaliana

text: 24h rep1


E-MTAB-12532:24h rep2

text: Walter and Eliza Hall Institute

text: public

text: E-MTAB-12532:24h rep2

text: ArrayExpress

characteristic: broker name

text: thale cress

characteristic: description

text: seed germination stage

characteristic: developmental stage

characteristic: ecotype

text: wild type genotype

characteristic: genotype

text: 24h germinating in the light

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: embryo

text: Arabidopsis thaliana

text: 24h rep2


E-MTAB-12532:48h rep1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Walter and Eliza Hall Institute

text: public

text: E-MTAB-12532:48h rep1

text: ArrayExpress

text: thale cress

text: seed germination stage

text: wild type genotype

text: 48h germinating in the light

characteristic: growth condition

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: embryo

characteristic: organism part

characteristic: replicate

text: Arabidopsis thaliana

text: 48h rep1


E-MTAB-12532:48h rep2

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Walter and Eliza Hall Institute

characteristic: INSDC center name

text: public

text: E-MTAB-12532:48h rep2

text: ArrayExpress

text: thale cress

characteristic: common name

text: seed germination stage

text: wild type genotype

text: 48h germinating in the light

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis thaliana

text: embryo

text: Arabidopsis thaliana

text: 48h rep2


text: UNIVERSITY OF OULU

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:UNIVERSITY OF OULU

characteristic: broad-scale environmental context

characteristic: collection date

unit: m

characteristic: depth

text: Pilot 1 (peat), surface peat, unfrozen, DNA

characteristic: elevation

characteristic: environmental medium

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: local environmental context

text: wetland metagenome

text: Microbial communities in reed and peat wetlands used for treatment of municipal wastewater

characteristic: project name

text: wetland metagenome

text: P1_PHSSF_pilot_surface_peat_unfrozen_DNA

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF OULU

characteristic: INSDC center name

text: public

characteristic: Submitter Id

text: Pilot 1 (peat), surface peat, unfrozen, RNA

characteristic: description

text: Finland

text: wetland metagenome

text: wetland metagenome

text: P1_PHSSF_pilot_surface_peat_unfrozen_RNA


text: UNIVERSITY OF OULU

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: broad-scale environmental context

characteristic: collection date

text: Pilot 1 (peat), deeper peat, unfrozen, DNA

text: Finland

text: wetland metagenome

characteristic: organism

text: wetland metagenome


text: UNIVERSITY OF OULU

text: public

characteristic: depth

text: Pilot 1 (peat), deeper peat, unfrozen, RNA

characteristic: elevation

characteristic: environmental medium

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: local environmental context

text: wetland metagenome

text: wetland metagenome


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF OULU

characteristic: INSDC center name

text: public

text: Ruka full scale peat wetland, surface peat, unfrozen, DNA

text: Finland

text: wetland metagenome

characteristic: project name

text: wetland metagenome

text: Ruka_PHSSF_full_scale_surface_peat_unfrozen_DNA

characteristic: title


text: UNIVERSITY OF OULU

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: broad-scale environmental context

text: Ruka full scale peat wetland, surface peat, unfrozen, RNA

characteristic: description

text: Finland

text: wetland metagenome

text: wetland metagenome

text: Ruka_PHSSF_full_scale_surface_peat_unfrozen_RNA


text: UNIVERSITY OF OULU

text: public

characteristic: collection date

characteristic: depth

text: Ruka full scale peat wetland, deeper peat, unfrozen, DNA

characteristic: elevation

characteristic: environmental medium

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: wetland metagenome

characteristic: organism

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