characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

text: public

text: Built environment

text: educational

text: urban

characteristic: environmental medium

text: HEPA filter

characteristic: filter type

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: heat pump

characteristic: heating and cooling system type

text: hallway

characteristic: indoor space

text: electric light

characteristic: light type

text: Indoor

characteristic: local environmental context

characteristic: occupancy at sampling

characteristic: occupant density at sampling

text: indoor metagenome

text: total DNA; 7.267875363 ng per mL; pico

characteristic: organism count

characteristic: project name

characteristic: relative air humidity

text: indoor metagenome

text: typically occupied

characteristic: space typical state

characteristic: typical occupant density

text: mechanical ventilation

characteristic: ventilation type


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

unit: kg

characteristic: absolute air humidity

unit: ºC

characteristic: air temperature

text: Built environment

characteristic: broad-scale environmental context

text: educational

characteristic: building occupancy type

text: urban

characteristic: building setting

unit: µmol/L

characteristic: carbon dioxide

characteristic: collection date

characteristic: description

text: porcelain

text: HEPA filter

text: United Kingdom

characteristic: geographic location (country and/or sea)

unit: DD

text: heat pump

text: bathroom

text: electric light

text: Indoor

text: indoor metagenome

characteristic: organism

text: total DNA; 7.732909505 ng per mL; pico

text: indoor metagenome

text: typically occupied

characteristic: title

text: mechanical ventilation


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

text: public

text: Built environment

text: educational

text: urban

text: porcelain

characteristic: environmental medium

text: HEPA filter

characteristic: filter type

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: heat pump

characteristic: heating and cooling system type

text: bathroom

characteristic: indoor space

text: electric light

characteristic: light type

text: Indoor

characteristic: local environmental context

characteristic: occupancy at sampling

characteristic: occupant density at sampling

text: indoor metagenome

text: total DNA; 4.456086649 ng per mL; pico

characteristic: organism count

characteristic: project name

characteristic: relative air humidity

text: indoor metagenome

text: typically occupied

characteristic: space typical state

characteristic: typical occupant density

text: mechanical ventilation

characteristic: ventilation type


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

characteristic: INSDC first public

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text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: absolute air humidity

characteristic: air temperature

text: Built environment

characteristic: broad-scale environmental context

text: educational

characteristic: building occupancy type

text: urban

characteristic: building setting

characteristic: carbon dioxide

characteristic: collection date

characteristic: description

text: porcelain

text: HEPA filter

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: heat pump

text: bathroom

text: electric light

text: Indoor

text: indoor metagenome

characteristic: organism

text: total DNA; 3.219919943 ng per mL; pico

text: indoor metagenome

text: typically occupied

characteristic: title

text: mechanical ventilation


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

text: public

text: Built environment

text: educational

text: urban

text: plastic

characteristic: environmental medium

text: HEPA filter

characteristic: filter type

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: heat pump

characteristic: heating and cooling system type

text: bathroom

characteristic: indoor space

text: electric light

characteristic: light type

text: Indoor

characteristic: local environmental context

characteristic: occupancy at sampling

characteristic: occupant density at sampling

text: indoor metagenome

text: total DNA; 7.08931795 ng per mL; pico

characteristic: organism count

characteristic: project name

characteristic: relative air humidity

text: indoor metagenome

text: typically occupied

characteristic: space typical state

characteristic: typical occupant density

text: mechanical ventilation

characteristic: ventilation type


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: absolute air humidity

characteristic: air temperature

text: Built environment

characteristic: broad-scale environmental context

text: educational

characteristic: building occupancy type

text: urban

characteristic: building setting

characteristic: carbon dioxide

characteristic: collection date

characteristic: description

text: plastic

text: HEPA filter

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: heat pump

text: bathroom

text: electric light

text: Indoor

text: indoor metagenome

characteristic: organism

text: total DNA; 3.457342438 ng per mL; pico

text: indoor metagenome

text: typically occupied

characteristic: title

text: mechanical ventilation


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

text: public

text: Built environment

text: educational

text: urban

text: plastic

characteristic: environmental medium

text: HEPA filter

characteristic: filter type

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: heat pump

characteristic: heating and cooling system type

text: bathroom

characteristic: indoor space

text: electric light

characteristic: light type

text: Indoor

characteristic: local environmental context

characteristic: occupancy at sampling

characteristic: occupant density at sampling

text: indoor metagenome

text: total DNA; 3.188525233 ng per mL; pico

characteristic: organism count

characteristic: project name

characteristic: relative air humidity

text: indoor metagenome

text: typically occupied

characteristic: space typical state

characteristic: typical occupant density

text: mechanical ventilation

characteristic: ventilation type


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: absolute air humidity

characteristic: air temperature

text: Built environment

characteristic: broad-scale environmental context

text: educational

characteristic: building occupancy type

text: urban

characteristic: building setting

characteristic: carbon dioxide

characteristic: collection date

text: control

characteristic: description

text: control

text: HEPA filter

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: heat pump

text: foyer

text: electric light

text: Control

text: indoor metagenome

characteristic: organism

text: total DNA; 6.494780629 ng per mL; pico

text: indoor metagenome

text: typically occupied

characteristic: title

text: mechanical ventilation


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

text: public

text: Built environment

text: educational

text: urban

text: control

text: control

characteristic: environmental medium

text: HEPA filter

characteristic: filter type

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: heat pump

characteristic: heating and cooling system type

text: foyer

characteristic: indoor space

text: electric light

characteristic: light type

text: Control

characteristic: local environmental context

characteristic: occupancy at sampling

characteristic: occupant density at sampling

text: indoor metagenome

text: total DNA; 6.05329252 ng per mL; pico

characteristic: organism count

characteristic: project name

characteristic: relative air humidity

text: indoor metagenome

text: typically occupied

characteristic: space typical state

characteristic: typical occupant density

text: mechanical ventilation

characteristic: ventilation type


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: absolute air humidity

characteristic: air temperature

text: Built environment

characteristic: broad-scale environmental context

text: educational

characteristic: building occupancy type

text: urban

characteristic: building setting

characteristic: carbon dioxide

characteristic: collection date

text: control

characteristic: description

text: control

text: HEPA filter

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: heat pump

text: foyer

text: electric light

text: Control

text: indoor metagenome

characteristic: organism

text: total DNA; 86.6670591 ng per mL; pico

text: indoor metagenome

text: typically occupied

characteristic: title

text: mechanical ventilation


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

text: public

text: Built environment

text: educational

text: urban

text: control

text: control

characteristic: environmental medium

text: HEPA filter

characteristic: filter type

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: heat pump

characteristic: heating and cooling system type

text: foyer

characteristic: indoor space

text: electric light

characteristic: light type

text: Control

characteristic: local environmental context

characteristic: occupancy at sampling

characteristic: occupant density at sampling

text: indoor metagenome

text: total DNA; 9.783376501 ng per mL; pico

characteristic: organism count

characteristic: project name

characteristic: relative air humidity

text: indoor metagenome

text: typically occupied

characteristic: space typical state

characteristic: typical occupant density

text: mechanical ventilation

characteristic: ventilation type


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: absolute air humidity

characteristic: air temperature

text: Built environment

characteristic: broad-scale environmental context

text: educational

characteristic: building occupancy type

text: urban

characteristic: building setting

characteristic: carbon dioxide

characteristic: collection date

text: control

characteristic: description

text: control

text: HEPA filter

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: heat pump

text: foyer

text: electric light

text: Control

text: indoor metagenome

characteristic: organism

text: total DNA; 98.91884467 ng per mL; pico

text: indoor metagenome

text: typically occupied

characteristic: title

text: mechanical ventilation


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

text: public

text: Built environment

text: educational

text: urban

text: control

text: control

characteristic: environmental medium

text: HEPA filter

characteristic: filter type

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: heat pump

characteristic: heating and cooling system type

text: foyer

characteristic: indoor space

text: electric light

characteristic: light type

text: Control

characteristic: local environmental context

characteristic: occupancy at sampling

characteristic: occupant density at sampling

text: indoor metagenome

text: total DNA; 7.157993878 ng per mL; pico

characteristic: organism count

characteristic: project name

characteristic: relative air humidity

text: indoor metagenome

text: typically occupied

characteristic: space typical state

characteristic: typical occupant density

text: mechanical ventilation

characteristic: ventilation type


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

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text: public

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characteristic: SRA accession

characteristic: Submitter Id

characteristic: absolute air humidity

characteristic: air temperature

text: Built environment

characteristic: broad-scale environmental context

text: educational

characteristic: building occupancy type

text: urban

characteristic: building setting

characteristic: carbon dioxide

characteristic: collection date

text: control

characteristic: description

text: control

text: HEPA filter

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: heat pump

text: foyer

text: electric light

text: Control

text: indoor metagenome

characteristic: organism

text: total DNA; 24.81359391 ng per mL; pico

text: indoor metagenome

text: typically occupied

characteristic: title

text: mechanical ventilation


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

text: public

text: Built environment

text: educational

text: urban

text: control

text: control

characteristic: environmental medium

text: HEPA filter

characteristic: filter type

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: heat pump

characteristic: heating and cooling system type

text: foyer

characteristic: indoor space

text: electric light

characteristic: light type

text: Control

characteristic: local environmental context

characteristic: occupancy at sampling

characteristic: occupant density at sampling

text: indoor metagenome

text: total DNA; 5.156581116 ng per mL; pico

characteristic: organism count

characteristic: project name

characteristic: relative air humidity

text: indoor metagenome

text: typically occupied

characteristic: space typical state

characteristic: typical occupant density

text: mechanical ventilation

characteristic: ventilation type


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: absolute air humidity

characteristic: air temperature

text: Built environment

characteristic: broad-scale environmental context

text: educational

characteristic: building occupancy type

text: urban

characteristic: building setting

characteristic: carbon dioxide

characteristic: collection date

text: control

characteristic: description

text: control

text: HEPA filter

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: heat pump

text: foyer

text: electric light

text: Control

text: indoor metagenome

characteristic: organism

text: total DNA; 7.62302802 ng per mL; pico

text: indoor metagenome

text: typically occupied

characteristic: title

text: mechanical ventilation


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

text: public

text: Built environment

text: educational

text: urban

text: control

text: control

characteristic: environmental medium

text: HEPA filter

characteristic: filter type

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: heat pump

characteristic: heating and cooling system type

text: foyer

characteristic: indoor space

text: electric light

characteristic: light type

text: Control

characteristic: local environmental context

characteristic: occupancy at sampling

characteristic: occupant density at sampling

text: indoor metagenome

text: total DNA; 93.68770112 ng per mL; pico

characteristic: organism count

characteristic: project name

characteristic: relative air humidity

text: indoor metagenome

text: typically occupied

characteristic: space typical state

characteristic: typical occupant density

text: mechanical ventilation

characteristic: ventilation type


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: absolute air humidity

characteristic: air temperature

text: Built environment

characteristic: broad-scale environmental context

text: educational

characteristic: building occupancy type

text: urban

characteristic: building setting

characteristic: carbon dioxide

characteristic: collection date

text: control

characteristic: description

text: control

text: HEPA filter

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: heat pump

text: foyer

text: electric light

text: Control

text: indoor metagenome

characteristic: organism

text: total DNA; 4.128404364 ng per mL; pico

text: indoor metagenome

text: typically occupied

characteristic: title

text: mechanical ventilation


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

text: public

text: Built environment

text: educational

text: urban

text: control

text: control

characteristic: environmental medium

text: HEPA filter

characteristic: filter type

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: heat pump

characteristic: heating and cooling system type

text: foyer

characteristic: indoor space

text: electric light

characteristic: light type

text: Control

characteristic: local environmental context

characteristic: occupancy at sampling

characteristic: occupant density at sampling

text: indoor metagenome

text: total DNA; 100.9889334 ng per mL; pico

characteristic: organism count

characteristic: project name

characteristic: relative air humidity

text: indoor metagenome

text: typically occupied

characteristic: space typical state

characteristic: typical occupant density

text: mechanical ventilation

characteristic: ventilation type


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: absolute air humidity

characteristic: air temperature

text: Built environment

characteristic: broad-scale environmental context

text: educational

characteristic: building occupancy type

text: urban

characteristic: building setting

characteristic: carbon dioxide

characteristic: collection date

text: control

characteristic: description

text: control

text: HEPA filter

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: heat pump

text: foyer

text: electric light

text: Control

text: indoor metagenome

characteristic: organism

text: total DNA; 18.98987521 ng per mL; pico

text: indoor metagenome

text: typically occupied

characteristic: title

text: mechanical ventilation


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

text: public

text: Built environment

text: educational

text: urban

text: control

text: control

characteristic: environmental medium

text: HEPA filter

characteristic: filter type

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: heat pump

characteristic: heating and cooling system type

text: foyer

characteristic: indoor space

text: electric light

characteristic: light type

text: Control

characteristic: local environmental context

characteristic: occupancy at sampling

characteristic: occupant density at sampling

text: indoor metagenome

text: total DNA; 3.429872067 ng per mL; pico

characteristic: organism count

characteristic: project name

characteristic: relative air humidity

text: indoor metagenome

text: typically occupied

characteristic: space typical state

characteristic: typical occupant density

text: mechanical ventilation

characteristic: ventilation type


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: absolute air humidity

characteristic: air temperature

text: Built environment

characteristic: broad-scale environmental context

text: educational

characteristic: building occupancy type

text: urban

characteristic: building setting

characteristic: carbon dioxide

characteristic: collection date

text: control

characteristic: description

text: control

text: HEPA filter

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: heat pump

text: foyer

text: electric light

text: Control

text: indoor metagenome

characteristic: organism

text: total DNA; 7.30908092 ng per mL; pico

text: indoor metagenome

text: typically occupied

characteristic: title

text: mechanical ventilation


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

text: public

text: Built environment

text: educational

text: urban

text: control

text: control

characteristic: environmental medium

text: HEPA filter

characteristic: filter type

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: heat pump

characteristic: heating and cooling system type

text: foyer

characteristic: indoor space

text: electric light

characteristic: light type

text: Control

characteristic: local environmental context

characteristic: occupancy at sampling

characteristic: occupant density at sampling

text: indoor metagenome

text: total DNA; 13.87449965 ng per mL; pico

characteristic: organism count

characteristic: project name

characteristic: relative air humidity

text: indoor metagenome

text: typically occupied

characteristic: space typical state

characteristic: typical occupant density

text: mechanical ventilation

characteristic: ventilation type


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: absolute air humidity

characteristic: air temperature

text: Built environment

characteristic: broad-scale environmental context

text: educational

characteristic: building occupancy type

text: urban

characteristic: building setting

characteristic: carbon dioxide

characteristic: collection date

text: control

characteristic: description

text: control

text: HEPA filter

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: heat pump

text: foyer

text: electric light

text: Control

text: indoor metagenome

characteristic: organism

text: total DNA; 48.29879915 ng per mL; pico

text: indoor metagenome

text: typically occupied

characteristic: title

text: mechanical ventilation


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

text: public

text: Built environment

text: educational

text: urban

text: control

text: control

characteristic: environmental medium

text: HEPA filter

characteristic: filter type

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: heat pump

characteristic: heating and cooling system type

text: foyer

characteristic: indoor space

text: electric light

characteristic: light type

text: Control

characteristic: local environmental context

characteristic: occupancy at sampling

characteristic: occupant density at sampling

text: indoor metagenome

text: total DNA; 13.42516286 ng per mL; pico

characteristic: organism count

characteristic: project name

characteristic: relative air humidity

text: indoor metagenome

text: typically occupied

characteristic: space typical state

characteristic: typical occupant density

text: mechanical ventilation

characteristic: ventilation type


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: absolute air humidity

characteristic: air temperature

text: Built environment

characteristic: broad-scale environmental context

text: educational

characteristic: building occupancy type

text: urban

characteristic: building setting

characteristic: carbon dioxide

characteristic: collection date

text: control

characteristic: description

text: control

text: HEPA filter

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: heat pump

text: foyer

text: electric light

text: Control

text: indoor metagenome

characteristic: organism

text: total DNA; 21.95471313 ng per mL; pico

text: indoor metagenome

text: typically occupied

characteristic: title

text: mechanical ventilation


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

text: public

text: Built environment

text: educational

text: urban

text: control

text: control

characteristic: environmental medium

text: HEPA filter

characteristic: filter type

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: heat pump

characteristic: heating and cooling system type

text: foyer

characteristic: indoor space

text: electric light

characteristic: light type

text: Control

characteristic: local environmental context

characteristic: occupancy at sampling

characteristic: occupant density at sampling

text: indoor metagenome

text: total DNA; 41.19182168 ng per mL; pico

characteristic: organism count

characteristic: project name

characteristic: relative air humidity

text: indoor metagenome

text: typically occupied

characteristic: space typical state

characteristic: typical occupant density

text: mechanical ventilation

characteristic: ventilation type


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: absolute air humidity

characteristic: air temperature

text: Built environment

characteristic: broad-scale environmental context

text: educational

characteristic: building occupancy type

text: urban

characteristic: building setting

characteristic: carbon dioxide

characteristic: collection date

text: control

characteristic: description

text: control

text: HEPA filter

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: heat pump

text: foyer

text: electric light

text: Control

text: indoor metagenome

characteristic: organism

text: total DNA; 10.92535908 ng per mL; pico

text: indoor metagenome

text: typically occupied

characteristic: title

text: mechanical ventilation


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

text: public

text: Built environment

text: educational

text: urban

text: control

text: control

characteristic: environmental medium

text: HEPA filter

characteristic: filter type

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: heat pump

characteristic: heating and cooling system type

text: foyer

characteristic: indoor space

text: electric light

characteristic: light type

text: Control

characteristic: local environmental context

characteristic: occupancy at sampling

characteristic: occupant density at sampling

text: indoor metagenome

text: total DNA; 4.136253041 ng per mL; pico

characteristic: organism count

characteristic: project name

characteristic: relative air humidity

text: indoor metagenome

text: typically occupied

characteristic: space typical state

characteristic: typical occupant density

text: mechanical ventilation

characteristic: ventilation type


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: absolute air humidity

characteristic: air temperature

text: Built environment

characteristic: broad-scale environmental context

text: educational

characteristic: building occupancy type

text: urban

characteristic: building setting

characteristic: carbon dioxide

characteristic: collection date

text: control

characteristic: description

text: control

text: HEPA filter

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: heat pump

text: foyer

text: electric light

text: Control

text: indoor metagenome

characteristic: organism

text: total DNA; 50.54155875 ng per mL; pico

text: indoor metagenome

text: typically occupied

characteristic: title

text: mechanical ventilation


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

text: public

text: Built environment

text: educational

text: urban

text: control

text: control

characteristic: environmental medium

text: HEPA filter

characteristic: filter type

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: heat pump

characteristic: heating and cooling system type

text: foyer

characteristic: indoor space

text: electric light

characteristic: light type

text: Control

characteristic: local environmental context

characteristic: occupancy at sampling

characteristic: occupant density at sampling

text: indoor metagenome

text: total DNA; 36.88878424 ng per mL; pico

characteristic: organism count

characteristic: project name

characteristic: relative air humidity

text: indoor metagenome

text: typically occupied

characteristic: space typical state

characteristic: typical occupant density

text: mechanical ventilation

characteristic: ventilation type


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: absolute air humidity

characteristic: air temperature

text: Built environment

characteristic: broad-scale environmental context

text: educational

characteristic: building occupancy type

text: urban

characteristic: building setting

characteristic: carbon dioxide

characteristic: collection date

text: control

characteristic: description

text: control

text: HEPA filter

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: heat pump

text: foyer

text: electric light

text: Control

text: indoor metagenome

characteristic: organism

text: total DNA; 9.932501374 ng per mL; pico

text: indoor metagenome

text: typically occupied

characteristic: title

text: mechanical ventilation


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

text: public

text: Built environment

text: educational

text: urban

text: control

text: control

characteristic: environmental medium

text: HEPA filter

characteristic: filter type

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: heat pump

characteristic: heating and cooling system type

text: foyer

characteristic: indoor space

text: electric light

characteristic: light type

text: Control

characteristic: local environmental context

characteristic: occupancy at sampling

characteristic: occupant density at sampling

text: indoor metagenome

text: total DNA; 16.30170316 ng per mL; pico

characteristic: organism count

characteristic: project name

characteristic: relative air humidity

text: indoor metagenome

text: typically occupied

characteristic: space typical state

characteristic: typical occupant density

text: mechanical ventilation

characteristic: ventilation type


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: absolute air humidity

characteristic: air temperature

text: Built environment

characteristic: broad-scale environmental context

text: educational

characteristic: building occupancy type

text: urban

characteristic: building setting

characteristic: carbon dioxide

characteristic: collection date

text: control

characteristic: description

text: control

text: HEPA filter

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: heat pump

text: foyer

text: electric light

text: Control

text: indoor metagenome

characteristic: organism

text: total DNA; 49.9489836 ng per mL; pico

text: indoor metagenome

text: typically occupied

characteristic: title

text: mechanical ventilation


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

text: public

text: Built environment

text: educational

text: urban

text: control

text: control

characteristic: environmental medium

text: HEPA filter

characteristic: filter type

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: heat pump

characteristic: heating and cooling system type

text: foyer

characteristic: indoor space

text: electric light

characteristic: light type

text: Control

characteristic: local environmental context

characteristic: occupancy at sampling

characteristic: occupant density at sampling

text: indoor metagenome

text: total DNA; 36.87112472 ng per mL; pico

characteristic: organism count

characteristic: project name

characteristic: relative air humidity

text: indoor metagenome

text: typically occupied

characteristic: space typical state

characteristic: typical occupant density

text: mechanical ventilation

characteristic: ventilation type


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: absolute air humidity

characteristic: air temperature

text: Built environment

characteristic: broad-scale environmental context

text: educational

characteristic: building occupancy type

text: urban

characteristic: building setting

characteristic: carbon dioxide

characteristic: collection date

text: control

characteristic: description

text: control

text: HEPA filter

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: heat pump

text: foyer

text: electric light

text: Control

text: indoor metagenome

characteristic: organism

text: total DNA; 41.84129974 ng per mL; pico

text: indoor metagenome

text: typically occupied

characteristic: title

text: mechanical ventilation


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

text: public

text: Built environment

text: educational

text: urban

text: control

text: control

characteristic: environmental medium

text: HEPA filter

characteristic: filter type

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: heat pump

characteristic: heating and cooling system type

text: foyer

characteristic: indoor space

text: electric light

characteristic: light type

text: Control

characteristic: local environmental context

characteristic: occupancy at sampling

characteristic: occupant density at sampling

text: indoor metagenome

text: total DNA; 26.77380111 ng per mL; pico

characteristic: organism count

characteristic: project name

characteristic: relative air humidity

text: indoor metagenome

text: typically occupied

characteristic: space typical state

characteristic: typical occupant density

text: mechanical ventilation

characteristic: ventilation type


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: absolute air humidity

characteristic: air temperature

text: Built environment

characteristic: broad-scale environmental context

text: educational

characteristic: building occupancy type

text: urban

characteristic: building setting

characteristic: carbon dioxide

characteristic: collection date

text: control

characteristic: description

text: control

text: HEPA filter

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: heat pump

text: foyer

text: electric light

text: Control

text: indoor metagenome

characteristic: organism

text: total DNA; 4.35994035 ng per mL; pico

text: indoor metagenome

text: typically occupied

characteristic: title

text: mechanical ventilation


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

text: public

text: Built environment

text: educational

text: urban

text: control

text: control

characteristic: environmental medium

text: HEPA filter

characteristic: filter type

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: heat pump

characteristic: heating and cooling system type

text: foyer

characteristic: indoor space

text: electric light

characteristic: light type

text: Control

characteristic: local environmental context

characteristic: occupancy at sampling

characteristic: occupant density at sampling

text: indoor metagenome

text: total DNA; 33.60215054 ng per mL; pico

characteristic: organism count

characteristic: project name

characteristic: relative air humidity

text: indoor metagenome

text: typically occupied

characteristic: space typical state

characteristic: typical occupant density

text: mechanical ventilation

characteristic: ventilation type


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Human

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: description

text: Milk

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Secretion

text: clinical metagenome

characteristic: organism

text: clinical metagenome

text: NextSeq 500

characteristic: sequencing method

characteristic: title


characteristic: ENA-CHECKLIST

text: Neupert et al., 2009

characteristic: Further Details

text: public

tag: Namespace:Department of Plant Sciences, University of Cambridge

text: Lab strain UVM4 obtained from Ralph Bock

text: Tris-acetate phosphate (TAP) medium with Kropat's trace elements (Kropat et al., 2011) but excluding selenium 25�C under a continuous or 16 h / 8 h light / dark cycle (shaking speed of 120 rpm, light intensity of 90 �E/(m^2*s^1)

characteristic: growth condition

text: Chlamydomonas reinhardtii

text: Under natural light at room temperature

characteristic: sample storage conditions

text: Chlamydomonas reinhardtii

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Sayer at al., 2023

text: Department of Plant Sciences, University of Cambridge

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: C. reinhardtii strain UVM4 (Neupert et al.. 2011) transformed with plasmid pAS_R1 containing a paromomycin resistance gene (aphVIII) under control of PMETE.

text: Tris-acetate phosphate (TAP) medium with Kropat's trace elements (Kropat et al., 2011) but excluding selenium. Paromomycin 5-50 �g/ml. 25�C under a continuous or 16 h / 8 h light / dark cycle (shaking speed of 120 rpm, light intensity of 90 �E/(m^2*s^1)

text: Chlamydomonas reinhardtii

text: Under natural light at room temperature

text: Chlamydomonas reinhardtii


IM4 / G2

text: Sayer at al., 2023

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: IM4 / G2

characteristic: Submitter Id

text: Insertional mutant obtained by transforming UVM4::pAS_R1 (UVM4-T12) with plasmid pHyg3 containing a hygromycin resistance gene (aphVII) under the control of the constitutively expressed b2-tubulin promoter.

text: Tris-acetate phosphate (TAP) medium with Kropat's trace elements (Kropat et al., 2011) but excluding selenium. Paromomycin 5-20 �g/ml, hygromycin 10-20 �g/ml. 25�C under a continuous or 16 h / 8 h light / dark cycle (shaking speed of 120 rpm, light intensity of 90 �E/(m^2*s^1)

text: Chlamydomonas reinhardtii

text: Under natural light at room temperature

text: Chlamydomonas reinhardtii


text: Sanger Institute

characteristic: GAL

text: NOT PROVIDED

text: Tree of Life Programme

text: public

tag: Namespace:Tree of Life Programme

text: EVA VAN DER HEIJDEN

text: Sanger Institute

characteristic: collecting institution

characteristic: collection date

unit: m

characteristic: elevation

text: Colombia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Putumayo | Mocoa | Campucana

characteristic: geographic location (region and locality)

text: Tropical rainforest

characteristic: habitat

text: EVA VAN DER HEIJDEN

text: Sanger Institute

text: adult

characteristic: lifestage

text: Melinaea mothone

characteristic: organism

text: WHOLE ORGANISM

characteristic: organism part

text: ToL

characteristic: project name

text: Melinaea mothone

text: FEMALE

characteristic: sex

text: N

characteristic: symbiont

characteristic: tolid


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Dalhousie University - Microbiology

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Dalhousie University - Microbiology

characteristic: Submitter Id

text: woodland biome

characteristic: broad-scale environmental context

characteristic: depth

text: Soil collected from forest post liming treatment

characteristic: description

text: soil

characteristic: environmental medium

text: Canada

text: temperate woodland biome

characteristic: local environmental context

text: soil metagenome

text: soil metagenome

characteristic: title


text: public

tag: Namespace:MAX PLANCK INSTITUTE FOR EVOLUTIONARY BIOLOGY

text: Tobias Kaiser

text: Max Planck Institute for Evolutionary Biology, August-Thienemann-Strasse 2, 24306 Ploen, Germany

characteristic: collecting institution

text: not available

characteristic: common name

text: Pooled individuals from the population Ros-2FM

text: Atlantic Ocean

characteristic: geographic location (country and/or sea)

text: 48¡ 43' 40'' N

characteristic: geographic location (latitude)

text: 3¡ 59' 06'' W

characteristic: geographic location (longitude)

text: Roscoff, France

characteristic: geographic location (region and locality)

text: Intertidal zones of rocky marine shores

characteristic: habitat

text: Tobias Kaiser

text: Max Planck Institute for Evolutionary Biology, August-Thienemann-Strasse 2, 24306 Plšn, Germany

text: Imago

characteristic: lifestage

text: Clunio marinus

characteristic: organism

text: Whole body

characteristic: organism part

text: An oligogenic architecture underlying ecological and reproductive divergence in sympatric populations

characteristic: project name

text: Clunio marinus

text: male

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: MAX PLANCK INSTITUTE FOR EVOLUTIONARY BIOLOGY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Tobias Kaiser

text: not available

text: Pooled individuals from the population Ros-2NM

text: Atlantic Ocean

text: 48¡ 43' 40'' N

text: 3¡ 59' 06'' W

text: Roscoff, France

text: Intertidal zones of rocky marine shores

text: Tobias Kaiser

text: Imago

text: Clunio marinus

text: Whole body

text: Clunio marinus

text: male


text: Wellcome Sanger Institute

text: public

tag: Namespace:Wellcome Sanger Institute

text: ArrayExpress

characteristic: broker name

text: peripheral blood mononuclear cell

text: human

characteristic: common name

text: Protocols: A vial of frozen PBMCs was acquired from Stemcell Technologies with informed consent (as stated by Stemcell Technologies) and approval from the Yorkshire & The Humber - Leeds East Research Ethics Committee (19/YH/0441). Frozen PBMCs were thawed in pre-warmed RF10 media, which was RPMI (Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS; Gibco) and penicillin/streptomycin (Sigma-Aldrich). Cells were pelleted by centrifugation at 500g for 5 min and resuspended in RF10 media, and split between two 10 cm petri dishes. Control PBMCs were then incubated in a total of 10 ml RF10 media at 37°C for 2 hr, whereas treated PBMCs were incubated in RF10 supplemented with cycloheximide (Sigma-Aldrich; final concentration of 100 μg/ml). After incubation, control and treated PBMCs were washed with ice cold RF10 and resuspended in 2% FBS in phosphate buffered saline (PBS; Gibco). For treated PBMCs, both the washing and resuspension buffer contained 100 μg/ml cycloheximide. Control and treated PBMCs were then loaded onto two separate channels of the Chromium chip from Chromium single cell V(D)J kit (10X Genomics 5′ v2) following the manufacturer’s instructions before droplet encapsulation on the Chromium controller. treatment with cycloheximide (Sigma-Aldrich; final concentration of 100 μg/ml) Single-cell cDNA synthesis was performed following the manufacturer's instructions. Single-cell gene expression (GEX) and targeted BCR and αβTCR libraries were generated following the manufacturer's instructions.

characteristic: description

text: adult

characteristic: developmental stage

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

characteristic: title


text: Wellcome Sanger Institute

text: public

text: ArrayExpress

text: peripheral blood mononuclear cell

text: human

text: Protocols: A vial of frozen PBMCs was acquired from Stemcell Technologies with informed consent (as stated by Stemcell Technologies) and approval from the Yorkshire & The Humber - Leeds East Research Ethics Committee (19/YH/0441). Frozen PBMCs were thawed in pre-warmed RF10 media, which was RPMI (Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS; Gibco) and penicillin/streptomycin (Sigma-Aldrich). Cells were pelleted by centrifugation at 500g for 5 min and resuspended in RF10 media, and split between two 10 cm petri dishes. Control PBMCs were then incubated in a total of 10 ml RF10 media at 37°C for 2 hr, whereas treated PBMCs were incubated in RF10 supplemented with cycloheximide (Sigma-Aldrich; final concentration of 100 μg/ml). After incubation, control and treated PBMCs were washed with ice cold RF10 and resuspended in 2% FBS in phosphate buffered saline (PBS; Gibco). For treated PBMCs, both the washing and resuspension buffer contained 100 μg/ml cycloheximide. Control and treated PBMCs were then loaded onto two separate channels of the Chromium chip from Chromium single cell V(D)J kit (10X Genomics 5′ v2) following the manufacturer’s instructions before droplet encapsulation on the Chromium controller. Single-cell cDNA synthesis was performed following the manufacturer's instructions. Single-cell gene expression (GEX) and targeted BCR and αβTCR libraries were generated following the manufacturer's instructions.

text: adult

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

tag: Namespace:University College London, Institute of Ophthalmology

text: ArrayExpress

text: embryonic stem cell

text: human

text: Protocols: CD29+/CD44+ cells were isolated from retinal organoids derived from human embryonic stem cells. Retinal organoids at different stages of development, including days 10, 20, 30, 50, 70 and 90 after initiation of retinal differentiation, were dissociated into single cells using a gentle cell dissociation reagent (Stem Cell Technologies, UK) (5mins at 37°C), and plated onto fibronectin coated tissue culture plates (50µg/ml) in the presence of fibroblast growth factor (FGF) (20ng/ml) and epidermal growth factor (EGF) (20ng/ml) in DMEM. After 3 days in culture, cells expressing the surface markers CD29 and CD44 that had selectively adhered to fibronectin, were detached, washed and total RNA was extracted. Retinal organoids were cultured according to the following publication DOI: 10.1002/sctm.18-0263 Total RNA was extracted using Qiagen Rneasy micro plus kit (Cat. 74034, Qiagen, UK) Samples were processed using the KAPA mRNA HyperPrep Kit (p/n KK8580).purified mRNA was fragmented using chemical hydrolysis (heat and divalent metal cation) and primed with random hexamers. Strand-specific first strand cDNA was generated using Reverse Transcriptase in the presence of Actinomycin D.The second cDNA strand was synthesised using dUTP in place of dTTP, to mark the second strand. The resultant cDNA was then “A-tailed” at the 3’ end to prevent self-ligation and adapter dimerisation. Full length xGen adaptors (IDT), containing two unique 8bp sample specific indexes, a unique molecular identifier (N8) and a T overhang were ligated to the A-Tailed cDNA. Successfully ligated cDNA molecules were then enriched with limited cycle PCR (12 cycles). The high fidelity polymerase employed in the PCR is unable to extend through uracil. This means only the first strand cDNA is amplified for sequencing, making the library strand specific (first-strand).

text: embryo

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: adherent culture

characteristic: growth condition

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: Homo sapiens


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University College London, Institute of Ophthalmology

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: embryonic stem cell

text: human

text: embryo

text: normal

text: wild type genotype

text: adherent culture

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: Homo sapiens


text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: Mueller cell

text: human

characteristic: common name

text: embryo

text: normal

text: wild type genotype

text: retinal organoid

text: CD29 positive; CD44 positive

characteristic: immunophenotype

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens


characteristic: ENA-CHECKLIST

text: Shanghai Tenth People's Hospital

text: public

tag: Namespace:Shanghai Tenth People's Hospital

text: ArrayExpress

text: Protocols: Strains were maintained at -80˚C. Strains were streaked on YPD plates. The plates were incubated at 37˚C overnight. Then several colonies were picked up and suspended in DDW. Cell density was adjusted to 10,000 cells/ml, then 100µl of cell suspension was plated on YPD plates. The plates were incubated at 37˚C overnight. Colonies were picked up and suspended in DDW, then cell pellets were obtained by centrifuging at highest speed for 1min. We used the phenol-chloroform method to extract Genomic DNA. Cell pellets were suspended in 200µl of lysis buffer, add 100µl of acid washed glass beads, and 0,2ml phenol:chloroform:isoamyl alcohol (25:24:1). Vortex for 30min at 4˚C. Add 200µl TE pH7.5. Centrifuge for 5min at maximum speed. Transfer aqueous layer to a new 1.5ml EP tube. Add 1ml 100% ethanol. Precipitate 1h at -20˚C. Pellet DNA by centrifuging for 10min at maximum speed. Discard the supernatant. Add 400µl TE pH 7.5. Add 3ul of RNase A (10mg/ml). Incubate at 37˚C for 30min. Add 10µl of 3M NaOAc and 1ml of 100% ethanol. Mix by inversion. Precipitate 30min at -20˚C. Pellet DNA by centrifuging for 10min at maximum speed. Discard supernatant. Air-dry the pellet overnight. Resuspend in 50µl water. 1µg Genomic DNA was randomly fragmented by Covaris, followed by fragments selection by Agencourt AMPure XP-Medium kit to an average size of 200~400 bp. Selected fragments were end repaired and 3\adenylate, then the adaptors were ligated to the end of these 3\adenylate fragments. The products were amplified by PCR and purified by the Agencourt AMPure XP-Medium kit. The purified double stranded PCR products were heat denatured to single strand, then circularised by the splint oligo sequence. The single strand circle DNA (ssCirDNA) were formatted as the final library and qualified by QC.

characteristic: description

text: wild type genotype

text: YPD plate

text: wild type karyotype

characteristic: karyotype

text: Cryptococcus neoformans var. grubii H99

characteristic: organism

text: Cryptococcus neoformans var. grubii H99

text: whole organism

characteristic: organism part

text: Cryptococcus neoformans var. grubii H99

characteristic: strain

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Shanghai Tenth People's Hospital

characteristic: INSDC center name

text: public

text: ArrayExpress

text: wild type genotype

characteristic: genotype

text: YPD plate

characteristic: growth condition

text: chromosome 4 disomy

text: Cryptococcus neoformans var. grubii H99

text: Cryptococcus neoformans var. grubii H99

text: whole organism

text: Cryptococcus neoformans var. grubii H99


text: Shanghai Tenth People's Hospital

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: wild type genotype

text: YPD plate

text: aneuploid

text: Cryptococcus neoformans var. grubii H99

text: Cryptococcus neoformans var. grubii H99

text: whole organism

text: Cryptococcus neoformans var. grubii H99


characteristic: ENA-CHECKLIST

text: public

tag: Namespace:Centre for Cancer Cell and Molecular Biology, Barts Cancer Institute, Queen Mary University of London

text: ArrayExpress

text: pancreatic ductal cell

text: house mouse

characteristic: common name

text: Protocols: The iCLIP method was performed as previously described in (Lee et al, 2021), with some modifications. Briefly, triplicates of mock transfected or WT Myc-Ncl, S4A Myc-Ncl, S4D Myc-Ncl transfected iKras PDAC cells were seeded onto 10 cm dishes (2 million per dish) and allowed to grow for 48 hrs in the presence of Dox (1µg/ml), before being irradiated once on ice with 150 mJ/cm2 UV light (254 nm) in PBS, using a Hoefer Scientific UV Crosslinker. Cells were then lysed in lysis buffer (50mM Tris-HCl pH 7.4, 100mM NaCl, 1% Igepal CA-630, 0.1% SDS, 0.5% sodium deoxycholate, supplemented with protease inhibitors), cleared, and diluted to a protein concentration of 1mg/ml. RNA was then digested with 0.2 U/ml of RNase I. Myc-tagged Ncl was then immunoprecipitated with 4g anti-Myc-tag antibody (Cell Signaling), pre-conjugated to protein G Dynabeads (Thermo). The RNAs were labelled at the 3’ end using an adapter (/5Phos/AG ATC GGA AGA GCG GTT CAG AAA AAA AAA AAA/iAzideN/AA AAA AAA AAA A/3Bio/) conjugated to an infrared dye to allow the visualization of the complexes on a gel. iKras cells were grown in DMEM supplemented with 10% FBS, 1% Penicillin/Streptomycin, and doxycycline (1µg/ml) to induce KrasG12D expression. Transient transfection with myc-tagged WT, S4A, and S4D Ncl constructs using Lipofectamine 2000 Barcoded and IR dye conjugated immunoprecipitates were resolved by SDS-PAGE and transferred onto nitrocellulose membrane. The region corresponding to 140-200 kDa protein-RNA crosslinked complexes of myc-Ncl was excised to isolate the associated RNAs. Isolated RNAs were reverse transcribed using primers containing experimental 5' barcodes unique to each sample(WT1: NNNN,TCCAC_0,NNN S4A1: NNNN,CCGGA_0,NNN S4D1: NNNN,AGGCA_0,NNN CTRL1: NNNN,GAATA_0,NNN WT2: NNNN,TTTAA_0,NNN S4A2: NNNN,CATTT_0,NNN S4D2: NNNN,ACCTT_0,NNN CTRL2: NNNN,GTATT_0,NNN WT3: NNNN,TGCGT_0,NNN S4A3: NNNN,CAAGT_0,NNN S4D3: NNNN,GCTCT_0,NNN CTRL3: NNNN,AGACT_0,NNN) (3'Adapter: AGATCGGAAG_1,AGCGGTTCAG_2).The cDNAs were then PCR amplified using Solexa P3 and P5 primers, gel extracted, and equal amounts of each amplified library was then combined together into a single mixed pool.

characteristic: description

text: adult

characteristic: developmental stage

text: pancreatic carcinoma

characteristic: disease

text: Mus musculus

text: Mus musculus

text: pancreas

text: Mus musculus


characteristic: ENA-FIRST-PUBLIC

text: Centre for Cancer Cell and Molecular Biology, Barts Cancer Institute, Queen Mary University of London

text: public

text: ArrayExpress

text: pancreatic ductal cell

text: house mouse

text: adult

text: pancreatic carcinoma

text: Mus musculus

characteristic: organism

text: Mus musculus

text: pancreas

characteristic: organism part

text: Mus musculus

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

tag: Namespace:CENTER FOR BIOTECHNOLOGY, BIELEFELD UNIVERSITY

text: rape

text: Brassica napus

text: Brassica napus


text: CENTER FOR BIOTECHNOLOGY, BIELEFELD UNIVERSITY

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: rape

text: Brassica napus

text: Brassica napus


Adriana

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Adriana

text: rape

text: Brassica napus

text: Brassica napus

text: BnaAdriana


Gaoyou

text: public

text: Gaoyou

characteristic: Submitter Id

text: rape

text: Brassica napus

text: Brassica napus

text: BnaGaoyou


Sollux

characteristic: ENA-CHECKLIST

text: public

text: Sollux

text: rape

text: Brassica napus

text: Brassica napus

text: BnaSollux


text: P&G Singapore Innovation Center

text: public

tag: Namespace:P&G Singapore Innovation Center

text: Japan

characteristic: geographic location (country and/or sea)

text: Osaka

characteristic: geographic location (region and locality)

text: metagenome

text: metagenome


NCC

text: P&G Singapore Innovation Center

text: public

text: NCC

characteristic: description

text: Japan

text: Osaka

text: Milli-Q _Water

text: metagenome

text: metagenome

text: NCC


AcoPraII-Sp

text: public

text: AcoPraII-Sp

text: AcoPraII-Sp

text: Frankia sp. AcoPraII.1

text: Frankia sp. AcoPraII.1

text: AcoPraII-Sp


Bee 33

text: Wales Veterinary Science Centre

text: public

text: Bee 33

tag: Namespace:Wales Veterinary Science Centre

text: Ronca et al.

text: white-tailed bumblebee

characteristic: common name

text: captured foraging individual

text: ATTCAACCAATCATAAAGATATTGG

characteristic: forward primer

text: United Kingdom

text: Rhos Glyn yr Helyg, Ceredigion

text: 52.1404, -4.1992

text: cytochrome oxidase 1

characteristic: locus

text: Bombus lucorum

text: TAAACTTCTGGATGTCCAAAAAATCA

characteristic: reverse primer

text: Bombus lucorum

text: Bumblebee


Bee 32

characteristic: ENA-FIRST-PUBLIC

text: Wales Veterinary Science Centre

text: public

text: Bee 32

text: Ronca et al.

text: captured foraging individual

text: ATTCAACCAATCATAAAGATATTGG

text: United Kingdom

text: Rhos Glyn yr Helyg, Ceredigion

text: 52.1404, -4.1992

text: cytochrome oxidase 1

text: Bombus hortorum

characteristic: organism

text: TAAACTTCTGGATGTCCAAAAAATCA

text: Bombus hortorum

text: Bumblebee

characteristic: title


Bee 48

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Wales Veterinary Science Centre

characteristic: INSDC center name

text: public

text: Bee 48

text: Ronca et al.

text: captured foraging individual

text: ATTCAACCAATCATAAAGATATTGG

text: United Kingdom

text: Rhos Glyn yr Helyg, Ceredigion

text: 52.1404, -4.1992

text: cytochrome oxidase 1

text: Bombus lapidarius

text: TAAACTTCTGGATGTCCAAAAAATCA

text: Bombus lapidarius

text: Bumblebee


Bee 50

text: Wales Veterinary Science Centre

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Bee 50

text: Ronca et al.

text: captured foraging individual

text: ATTCAACCAATCATAAAGATATTGG

text: United Kingdom

text: Rhos Glyn yr Helyg, Ceredigion

text: 52.1404, -4.1992

text: cytochrome oxidase 1

text: Bombus lapidarius

text: TAAACTTCTGGATGTCCAAAAAATCA

text: Bombus lapidarius

text: Bumblebee


Bee 73

characteristic: ENA-CHECKLIST

text: Wales Veterinary Science Centre

text: public

characteristic: SRA accession

text: Bee 73

characteristic: Submitter Id

text: Ronca et al.

text: captured foraging individual

text: ATTCAACCAATCATAAAGATATTGG

text: United Kingdom

text: Rhos Glyn yr Helyg, Ceredigion

text: 52.1404, -4.1992

text: cytochrome oxidase 1

text: Bombus cryptarum

text: TAAACTTCTGGATGTCCAAAAAATCA

text: Bombus cryptarum

text: Bumblebee


Bee 155

text: Wales Veterinary Science Centre

text: public

text: Bee 155

text: Ronca et al.

text: captured foraging individual

characteristic: description

text: ATTCAACCAATCATAAAGATATTGG

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Rhos Glyn yr Helyg, Ceredigion

characteristic: geographic location (region and locality)

text: 52.1404, -4.1992

text: cytochrome oxidase 1

text: Bombus pascuorum

text: TAAACTTCTGGATGTCCAAAAAATCA

text: Bombus pascuorum

text: Bumblebee


Bee 89

text: Wales Veterinary Science Centre

text: public

text: Bee 89

text: Ronca et al.

text: captured foraging individual

text: ATTCAACCAATCATAAAGATATTGG

characteristic: forward primer

text: United Kingdom

text: Rhos Glyn yr Helyg, Ceredigion

text: 52.1404, -4.1992

text: cytochrome oxidase 1

characteristic: locus

text: Bombus pascuorum

text: TAAACTTCTGGATGTCCAAAAAATCA

text: Bombus pascuorum

text: Bumblebee


Bee 90

characteristic: ENA-FIRST-PUBLIC

text: Wales Veterinary Science Centre

text: public

text: Bee 90

text: Ronca et al.

text: captured foraging individual

text: ATTCAACCAATCATAAAGATATTGG

text: United Kingdom

text: Rhos Glyn yr Helyg, Ceredigion

text: 52.1404, -4.1992

text: cytochrome oxidase 1

text: Bombus pascuorum

text: TAAACTTCTGGATGTCCAAAAAATCA

characteristic: reverse primer

text: Bombus pascuorum

text: Bumblebee


Bee 143

text: Wales Veterinary Science Centre

text: public

text: Bee 143

text: Ronca et al.

text: captured foraging individual

text: ATTCAACCAATCATAAAGATATTGG

text: United Kingdom

text: Rhos Glyn yr Helyg, Ceredigion

text: 52.1404, -4.1992

text: cytochrome oxidase 1

text: Bombus pratorum

characteristic: organism

text: TAAACTTCTGGATGTCCAAAAAATCA

text: Bombus pratorum

text: Bumblebee

characteristic: title


Bee 36

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Wales Veterinary Science Centre

text: public

text: Bee 36

text: Ronca et al.

text: buff-tailed bumblebee

characteristic: common name

text: captured foraging individual

text: ATTCAACCAATCATAAAGATATTGG

text: United Kingdom

text: Rhos Glyn yr Helyg, Ceredigion

text: 52.1404, -4.1992

text: cytochrome oxidase 1

text: Bombus terrestris

text: TAAACTTCTGGATGTCCAAAAAATCA

text: Bombus terrestris

text: Bumblebee


Bee 30

text: Wales Veterinary Science Centre

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Bee 30

text: Ronca et al.

text: buff-tailed bumblebee

text: captured foraging individual

text: ATTCAACCAATCATAAAGATATTGG

text: United Kingdom

text: Rhos Glyn yr Helyg, Ceredigion

text: 52.1404, -4.1992

text: cytochrome oxidase 1

text: Bombus terrestris

text: TAAACTTCTGGATGTCCAAAAAATCA

text: Bombus terrestris

text: Bumblebee


Bee 21

characteristic: ENA-CHECKLIST

text: Wales Veterinary Science Centre

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Bee 21

text: Ronca et al.

text: white-tailed bumblebee

text: captured foraging individual

text: ATTCAACCAATCATAAAGATATTGG

text: United Kingdom

text: Rhos Glandenys, Ceredigion

text: 52.2637, -4.1437

text: cytochrome oxidase 1

text: Bombus lucorum

text: TAAACTTCTGGATGTCCAAAAAATCA

text: Bombus lucorum

text: Bumblebee


Bee 1

text: Wales Veterinary Science Centre

text: public

text: Bee 1

characteristic: Submitter Id

text: Ronca et al.

text: captured foraging individual

characteristic: description

text: ATTCAACCAATCATAAAGATATTGG

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Rhos Glandenys, Ceredigion

text: 52.2637, -4.1437

text: cytochrome oxidase 1

text: Bombus hortorum

text: TAAACTTCTGGATGTCCAAAAAATCA

text: Bombus hortorum

text: Bumblebee


Bee 96

text: Wales Veterinary Science Centre

text: public

text: Bee 96

text: Ronca et al.

text: captured foraging individual

text: ATTCAACCAATCATAAAGATATTGG

characteristic: forward primer

text: United Kingdom

text: Rhos Glandenys, Ceredigion

characteristic: geographic location (region and locality)

text: 52.2637, -4.1437

text: cytochrome oxidase 1

characteristic: locus

text: Bombus lapidarius

text: TAAACTTCTGGATGTCCAAAAAATCA

text: Bombus lapidarius

text: Bumblebee


Bee 6

characteristic: ENA-FIRST-PUBLIC

text: Wales Veterinary Science Centre

text: public

text: Bee 6

text: Ronca et al.

text: captured foraging individual

text: ATTCAACCAATCATAAAGATATTGG

text: United Kingdom

text: Rhos Glandenys, Ceredigion

text: 52.2637, -4.1437

text: cytochrome oxidase 1

text: Bombus cryptarum

characteristic: organism

text: TAAACTTCTGGATGTCCAAAAAATCA

characteristic: reverse primer

text: Bombus cryptarum

text: Bumblebee


Bee 23

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Wales Veterinary Science Centre

text: public

text: Bee 23

text: Ronca et al.

text: captured foraging individual

text: ATTCAACCAATCATAAAGATATTGG

text: United Kingdom

text: Rhos Glandenys, Ceredigion

text: 52.2637, -4.1437

text: cytochrome oxidase 1

text: Bombus pascuorum

text: TAAACTTCTGGATGTCCAAAAAATCA

text: Bombus pascuorum

text: Bumblebee

characteristic: title


Bee 24

text: Wales Veterinary Science Centre

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Bee 24

text: Ronca et al.

text: captured foraging individual

text: ATTCAACCAATCATAAAGATATTGG

text: United Kingdom

text: Rhos Glandenys, Ceredigion

text: 52.2637, -4.1437

text: cytochrome oxidase 1

text: Bombus pascuorum

text: TAAACTTCTGGATGTCCAAAAAATCA

text: Bombus pascuorum

text: Bumblebee


Bee 168

characteristic: ENA-CHECKLIST

text: Wales Veterinary Science Centre

characteristic: INSDC last update

text: public

text: Bee 168

text: Ronca et al.

text: captured foraging individual

text: ATTCAACCAATCATAAAGATATTGG

text: United Kingdom

text: Rhos Glandenys, Ceredigion

text: 52.2637, -4.1437

text: cytochrome oxidase 1

text: Bombus pratorum

text: TAAACTTCTGGATGTCCAAAAAATCA

text: Bombus pratorum

text: Bumblebee


Bee 4

text: Wales Veterinary Science Centre

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Bee 4

text: Ronca et al.

text: buff-tailed bumblebee

characteristic: common name

text: captured foraging individual

text: ATTCAACCAATCATAAAGATATTGG

text: United Kingdom

text: Rhos Glandenys, Ceredigion

text: 52.2637, -4.1437

text: cytochrome oxidase 1

text: Bombus terrestris

text: TAAACTTCTGGATGTCCAAAAAATCA

text: Bombus terrestris

text: Bumblebee


Bee 126

text: Wales Veterinary Science Centre

text: public

text: Bee 126

characteristic: Submitter Id

text: Ronca et al.

text: buff-tailed bumblebee

text: captured foraging individual

characteristic: description

text: ATTCAACCAATCATAAAGATATTGG

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Rhos Glandenys, Ceredigion

text: 52.2637, -4.1437

text: cytochrome oxidase 1

text: Bombus terrestris

text: TAAACTTCTGGATGTCCAAAAAATCA

text: Bombus terrestris

text: Bumblebee


Bee 132

text: Wales Veterinary Science Centre

text: public

text: Bee 132

text: Ronca et al.

text: white-tailed bumblebee

text: captured foraging individual

text: ATTCAACCAATCATAAAGATATTGG

characteristic: forward primer

text: United Kingdom

text: Rhos Fullbrook, Ceredigion

characteristic: geographic location (region and locality)

text: 52.2472, -3.9539

text: cytochrome oxidase 1

characteristic: locus

text: Bombus lucorum

text: TAAACTTCTGGATGTCCAAAAAATCA

text: Bombus lucorum

text: Bumblebee


Bee 240

characteristic: ENA-FIRST-PUBLIC

text: Wales Veterinary Science Centre

text: public

text: Bee 240

text: Ronca et al.

text: captured foraging individual

text: ATTCAACCAATCATAAAGATATTGG

text: United Kingdom

text: Rhos Fullbrook, Ceredigion

text: 52.2472, -3.9539

text: cytochrome oxidase 1

text: Bombus hortorum

characteristic: organism

text: TAAACTTCTGGATGTCCAAAAAATCA

characteristic: reverse primer

text: Bombus hortorum

text: Bumblebee


Bee 9

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Wales Veterinary Science Centre

text: public

text: Bee 9

text: Ronca et al.

text: captured foraging individual

text: ATTCAACCAATCATAAAGATATTGG

text: United Kingdom

text: Rhos Fullbrook, Ceredigion

text: 52.2472, -3.9539

text: cytochrome oxidase 1

text: Bombus cryptarum

text: TAAACTTCTGGATGTCCAAAAAATCA

text: Bombus cryptarum

text: Bumblebee

characteristic: title


Bee 65

characteristic: ENA-CHECKLIST

text: Wales Veterinary Science Centre

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Bee 65

text: Ronca et al.

text: captured foraging individual

text: ATTCAACCAATCATAAAGATATTGG

text: United Kingdom

text: Rhos Fullbrook, Ceredigion

text: 52.2472, -3.9539

text: cytochrome oxidase 1

text: Bombus pascuorum

text: TAAACTTCTGGATGTCCAAAAAATCA

text: Bombus pascuorum

text: Bumblebee


Bee 64

text: Wales Veterinary Science Centre

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Bee 64

text: Ronca et al.

text: captured foraging individual

text: ATTCAACCAATCATAAAGATATTGG

text: United Kingdom

text: Rhos Fullbrook, Ceredigion

text: 52.2472, -3.9539

text: cytochrome oxidase 1

text: Bombus pascuorum

text: TAAACTTCTGGATGTCCAAAAAATCA

text: Bombus pascuorum

text: Bumblebee


Bee 224

text: Wales Veterinary Science Centre

text: public

text: Bee 224

characteristic: Submitter Id

text: Ronca et al.

text: captured foraging individual

characteristic: description

text: ATTCAACCAATCATAAAGATATTGG

text: United Kingdom

text: Rhos Fullbrook, Ceredigion

text: 52.2472, -3.9539

text: cytochrome oxidase 1

text: Bombus pascuorum

text: TAAACTTCTGGATGTCCAAAAAATCA

text: Bombus pascuorum

text: Bumblebee


Bee 136

text: Wales Veterinary Science Centre

text: public

text: Bee 136

text: Ronca et al.

text: captured foraging individual

text: ATTCAACCAATCATAAAGATATTGG

characteristic: forward primer

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Rhos Fullbrook, Ceredigion

text: 52.2472, -3.9539

text: cytochrome oxidase 1

text: Bombus pratorum

text: TAAACTTCTGGATGTCCAAAAAATCA

text: Bombus pratorum

text: Bumblebee


Bee 5

text: Wales Veterinary Science Centre

text: public

text: Bee 5

text: Ronca et al.

text: buff-tailed bumblebee

characteristic: common name

text: captured foraging individual

text: ATTCAACCAATCATAAAGATATTGG

text: United Kingdom

text: Rhos Fullbrook, Ceredigion

characteristic: geographic location (region and locality)

text: 52.2472, -3.9539

text: cytochrome oxidase 1

characteristic: locus

text: Bombus terrestris

text: TAAACTTCTGGATGTCCAAAAAATCA

text: Bombus terrestris

text: Bumblebee


Bee 10

characteristic: ENA-FIRST-PUBLIC

text: Wales Veterinary Science Centre

text: public

text: Bee 10

text: Ronca et al.

text: captured foraging individual

text: ATTCAACCAATCATAAAGATATTGG

text: United Kingdom

text: Rhos Fullbrook, Ceredigion

text: 52.2472, -3.9539

text: cytochrome oxidase 1

text: Bombus jonellus

characteristic: organism

text: TAAACTTCTGGATGTCCAAAAAATCA

characteristic: reverse primer

text: Bombus jonellus

text: Bumblebee


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

tag: Namespace:Institut d'Investigacio Sanitaria de Palma

text: Lineage_OriginalStrain_FdcMic_DayExposure_Mutant

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa

characteristic: title


characteristic: ENA-CHECKLIST

text: Institut d'Investigacio Sanitaria de Palma

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: Cancer Research UK Cambridge Institute.

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Cancer Research UK Cambridge Institute.

text: ArrayExpress

characteristic: broker name

text: house mouse

text: Protocols: Mouse embryos from the appropriate somite stages (8 to 35 somites) were dissected in RNAse-free conditions in cold PBS, on silicone plates. To dissect out the somites, embryos were treated with dispase II (1mg/mL in DMEM) for 30-45 seconds at 37°C. The three most posterior pairs of somites were then collected using tungsten needles, dissecting out every somite separately. We labelled each somite pair as somite I, II or III, from the most posterior to the most anterior, respectively. Each individual somite was placed in 10µL of lysis buffer (Takara) containing RNase inhibitor. One somite from each pair was flash frozen in liquid nitrogen and stored at -80°C for later processing for RNA-seq. The matching somites were directly processed to generate ATAC-seq libraries. Individual somites were transposed with 1µL of transposome (Nextera DNA Sample Preparation kit FC-121-1030) at 37°C for 30 minutes. Samples were then purified with the Zymo Clean & Concentrator kit and eluted in 21µL of elution buffer. Transposed DNA was quantified by qPCR using 5 μl of PCR products. The number of additional cycles was determined by plotting linear Rn versus cycle and corresponded to one third of the maximum fluorescence intensity. Transposed DNA was then amplified with 13 cycles of PCR. The final products were double size-selected with AMPure beads (0.55X - 1.5X) to obtain fragments between 100bp and 700bp. Libraries were quantified and the sizes were assessed with a TapeStation machine.

text: embryo stage

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

characteristic: individual

text: Mus musculus

text: Mus musculus

text: somite 21

characteristic: organism part

text: posterior

characteristic: sampling site

text: Mus musculus

text: female

characteristic: sex

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Cancer Research UK Cambridge Institute.

text: public

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: embryo stage

text: wild type genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: somite 21

text: medial

text: Mus musculus

text: female


characteristic: ENA-CHECKLIST

characteristic: External Id

text: Cancer Research UK Cambridge Institute.

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: house mouse

text: embryo stage

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: somite 21

text: anterior

text: Mus musculus

text: female

characteristic: title


text: Cancer Research UK Cambridge Institute.

text: public

characteristic: INSDC status

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