characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: description

text: third instar larva

characteristic: developmental stage

text: normal

text: wild type genotype

text: Drosophila suzukii

text: Drosophila suzukii

text: head

text: Drosophila suzukii

text: mixed


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

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characteristic: INSDC last update

text: public

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tag: Namespace:Department of Ecology & Evolution University of Luasanne

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unit: day

text: ArrayExpress

text: Protocols: All adult samples were collected from flies aged between 2-10 days. Antenna were collected by flash freezing flies in liquid nitrogen and agitating them over a sieve connected to a collection dish. Antenna were selected from the collection dish using a pipette under a dissecting scope. Forelegs and proboscis with maxillary palps were collected from individual files using forceps and microscalpels under a dissecting scope. Third instar larva were collected from vials by floating them in 75% sucrose water and washed. Larva heads were removed under a dissecting scope using a microscalpel. For each replicate, ~10 third instar larval heads, ~25 proboscis, ~50 legs, ~5 ovipositors and ~100 antennae were collected. After sorting sexes on C02, all flies were left in food vials for at least 24h before the dissections. All adult samples were collected from flies aged between 2-10 days. Antenna were collected by flash freezing flies in liquid nitrogen and agitating them over a sieve connected to a collection dish. Antenna were selected from the collection dish using a pipette under a dissecting scope. Forelegs and proboscis with maxillary palps were collected from individual files using forceps and microscalpels under a dissecting scope. Third instar larva were collected from vials by floating them in 75% sucrose water and washed. Larva heads were removed under a dissecting scope using a microscalpel. For each replicate, ~10 third instar larval heads, ~25 proboscis, ~50 legs, ~5 ovipositors and ~100 antennae were collected. After sorting sexes on C02, all flies were left in food vials for at least 24h before the dissections. Fly strains were reared on a standard yeast/cornmeal/agar medium supplemented with Carolina 4-24 Formula and maintained in a 12:12 hr light:dark cycle at 25 degrees. Adults between 2 to 10 days old were sex-sorted on CO2 at least 24h before the dissections. Dissected tissues were homogenized in 200ul of Trizol (Invitrogen) using a Precellys24 (6800rpm, 2x30s with 10s breaks ; Bertin Technology) followed by a standard Trizol RNA extraction. The final mRNA concentration was measured using a DeNovix Ds-11 FX spectrophotometer. mRNA libraries were prepared using KAPA Stranded mRNA-seq Kit (Roche) following manufacturer’s instructions (Version 5.17). Briefly, 500ng of total RNA diluted in 50ul of RNAse-free water was first placed on supplied mRNA capture magnetic beads to allow the isolation of mature, polyadenylated, mRNA which was subsequently fragmented to a size of 100-200bp. Double-strand cDNA was then synthesized, marked by A-tailing and barcoded with 2.5ul of TruSeq RNA UD Indexes (Illumina). SPRI select beads (Beckman Coulter) were used for cleanup.

text: third instar larva

text: normal

characteristic: disease

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text: Drosophila suzukii

characteristic: organism

text: Drosophila suzukii

text: head

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text: Drosophila suzukii

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characteristic: ENA-FIRST-PUBLIC

text: Department of Ecology & Evolution University of Luasanne

text: public

text: 2 to 10

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

text: wild type genotype

text: Drosophila erecta

text: Drosophila erecta

text: ovipositor

text: Drosophila erecta

text: female


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

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text: 2 to 10

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text: fruit fly

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text: Drosophila melanogaster

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characteristic: Submitter Id

text: 2 to 10

text: ArrayExpress

text: adult

text: normal

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text: Drosophila santomea

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text: adult

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text: wild type genotype

text: Drosophila sechellia

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text: Drosophila sechellia

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text: Drosophila simulans

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characteristic: ENA-FIRST-PUBLIC

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text: Drosophila erecta

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characteristic: ENA-FIRST-PUBLIC

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text: 2 to 10

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text: adult

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text: Drosophila santomea

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characteristic: ENA-CHECKLIST

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characteristic: description

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text: Drosophila suzukii

text: Drosophila suzukii

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text: Drosophila suzukii

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text: INRAE DE TOURS

text: public

characteristic: SRA accession

tag: Namespace:INRAE DE TOURS

characteristic: Submitter Id

text: poultry hatchery

characteristic: broad-scale environmental context

characteristic: collection date

text: chicken

characteristic: common name

text: Ceca, animal 1

text: microbial community

characteristic: environmental medium

text: France

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: gut

characteristic: local environmental context

text: Gallus gallus

text: PhD Lorna Agap�

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characteristic: ENA-CHECKLIST

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characteristic: geographic location (latitude)

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text: Gallus gallus

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text: INRAE DE TOURS

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text: public

text: poultry hatchery

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text: PhD Lorna Agap�

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text: INRAE DE TOURS

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text: poultry hatchery

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text: PhD Lorna Agap�

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characteristic: ENA-CHECKLIST

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text: INRAE DE TOURS

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text: chicken

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text: Ceca, animal 8

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text: France

characteristic: geographic location (country and/or sea)

text: gut

text: Gallus gallus

text: PhD Lorna Agap�

text: Gallus gallus


characteristic: ENA-FIRST-PUBLIC

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text: INRAE DE TOURS

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text: poultry hatchery

text: chicken

text: Ceca, animal 9

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characteristic: geographic location (latitude)

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text: INRAE DE TOURS

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text: PhD Lorna Agap�

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characteristic: ENA-FIRST-PUBLIC

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text: PhD Lorna Agap�

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text: INRAE DE TOURS

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characteristic: geographic location (latitude)

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text: PhD Lorna Agap�

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text: INRAE DE TOURS

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text: poultry hatchery

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characteristic: ENA-FIRST-PUBLIC

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text: INRAE DE TOURS

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text: poultry hatchery

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