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characteristic: broker name

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text: Samples prepared at Berkshire and Surrey Pathology Services Lighthouse Laboratory by Berkshire and Surrey Pathology Services Lighthouse Laboratory and Alex Alderton, Roberto Amato, Jeffrey Barrett, Sonia Goncalves, Ewan Harrison, David K. Jackson, Ian Johnston, Dominic Kwiatkowski, Cordelia Langford, John Sillitoe on behalf of the Wellcome Sanger Institute COVID-19 Surveillance Team

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characteristic: host common name

text: not provided

characteristic: host health state

text: Human

text: not provided

text: not provided

text: not provided


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: SC

text: not provided

text: United Kingdom

text: Scotland

text: Human

text: not provided

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status


text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: SC

characteristic: broker name

characteristic: collecting institution

text: not provided

characteristic: description

text: United Kingdom

text: England

text: Human

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: organism

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: SC

text: Lighthouse Lab in Glasgow

characteristic: collection date

text: not provided

characteristic: collector name

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

text: not provided

text: not provided

text: not provided


text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: SC

text: Lighthouse Laboratory Plymouth

text: not provided

text: Samples prepared at Lighthouse Laboratory Plymouth by Lighthouse Laboratory Plymouth and Alex Alderton, Roberto Amato, Jeffrey Barrett, Sonia Goncalves, Ewan Harrison, David K. Jackson, Ian Johnston, Dominic Kwiatkowski, Cordelia Langford, John Sillitoe on behalf of the Wellcome Sanger Institute COVID-19 Surveillance Team (https://www.sanger.ac.uk/covid-team)

text: United Kingdom

text: England

text: Human

text: not provided

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

tag: Namespace:Genetics and Genome Biology, SickKids Research Institute, Toronto, ON, Canada. Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada

characteristic: Submitter Id

unit: hour

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: Protocols: Drosophila melanogaster larvae were reared on standard molasses-cornmeal medium at 24⁰C and a 12 hr light/dark cycle with lights on at 0800hr. Larvae were reared to 72 ± 2 hrs old third instar and collected for treatment. The food-deprived treatment group was food-deprived on PBS-soaked filter paper for 4hrs immediately before dissections, the fed treatment group was placed food for 4 hrs. Central nervous systems (brain and brainstem, CNS), fat bodies, and intestines of 76 ± 2 hrs old third instar individual larvae were manually dissected in Schneider’s Insect Medium (Sigma-Aldrich S01460) and immediately placed on dry ice. Tissues of four individuals were pooled for intestine and fat body samples and six individuals for CNS samples. Total RNA was extracted using the Arcturus PicoPure RNA Isolation Kit (catalog no. KIT0204; Applied Biosystems) and the RNase-free DNase set (catalog no. 79254; Qiagen), with the following modifications from the manufacturer’s protocol: 100 μL of RNA extraction buffer was added to each sample and samples were incubated at 42 °C for 30 min. The manufacturer’s protocol for CapSure Macro LCM Caps was then followed starting at step 2, including the optional DNase treatment step. Samples quality control and integrity check was performed on a Bioanalyzer 2100 (Agilent) with the Agilent RNA 6000 nano kit. Libraries were prepared with the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (E7760) following PolyA enrichment for mRNA.

characteristic: description

text: third instar larva stage

characteristic: developmental stage

text: control

characteristic: diet

text: forR

characteristic: genotype

characteristic: individual

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: central nervous system

characteristic: organism part

text: rover larva

characteristic: phenotype

text: Drosophila melanogaster

text: mixed

characteristic: sex

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Genetics and Genome Biology, SickKids Research Institute, Toronto, ON, Canada. Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: fruit fly

text: third instar larva stage

text: control

text: forR

text: Drosophila melanogaster

text: Drosophila melanogaster

text: central nervous system

text: rover larva

text: Drosophila melanogaster

text: mixed