characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: not provided

text: Untreated wastewater was collected in Stockholm at Käppalaverket, Bromma reningsverk and Henriksdal reningsverk on 2022-05-23 over 24 hours using flow compensated samplers. The samples were then combined flow-proportionally into a composite sample for the city. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-KTH. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

text: Stockholm

text: virus

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: organism

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

text: 24 hour period, using a flow compensated sampler at each site, flow-proportionally combined into a single sample

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W21 Stockholm Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Swedish University of Agricultural Sciences

characteristic: Submitter Id

unit: L

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Malmö at Sjölunda reningsverk on 2022-05-23 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-KTH. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Malmö

characteristic: geographic location (region and locality)

text: virus

characteristic: investigation type

text: Sjölunda reningsverk

text: Ion AmpliSeq SARS-CoV-2 Insight Research Assay with SuperScript™ VILO™ cDNA Synthesis Kit using Ion Chef™ (Thermo Fisher, # A51306, # 11754050, # MAN0019277, Rev B.0, pp. 3-4, # MAN0024915 Rev B.0, Ch 4, pp. 42-43)

text: TNA concentration using Maxwell RSC Enviro Total Nucleic Acid Kit (Promega, # AS1831 & # TM663 Rev 4/22)

text: wastewater metagenome

unit: day

text: not provided

text: 24 hour period, using a flow compensated sampler

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: Severe acute respiratory syndrome coronavirus 2

text: 2022-W21 Malmö Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Swedish University of Agricultural Sciences

text: public

text: not provided

text: Untreated wastewater was collected in Stockholm at Käppalaverket, Bromma reningsverk and Henriksdal reningsverk on 2022-05-30 over 24 hours using flow compensated samplers. The samples were then combined flow-proportionally into a composite sample for the city. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-KTH. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

text: Stockholm

text: virus

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: organism

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W22 Stockholm Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Stockholm at Käppalaverket, Bromma reningsverk and Henriksdal reningsverk on 2022-06-06 over 24 hours using flow compensated samplers. The samples were then combined flow-proportionally into a composite sample for the city. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-KTH. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

text: Stockholm

characteristic: geographic location (region and locality)

text: virus

characteristic: investigation type

text: wastewater metagenome

text: not provided

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W23 Stockholm Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

text: public

text: not provided

text: Untreated wastewater was collected in Malmö at Sjölunda reningsverk on 2022-06-06 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-KTH. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Malmö

text: virus

text: Sjölunda reningsverk

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: organism

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W23 Malmö Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Stockholm at Käppalaverket, Bromma reningsverk and Henriksdal reningsverk on 2022-06-13 over 24 hours using flow compensated samplers. The samples were then combined flow-proportionally into a composite sample for the city. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-KTH. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

text: Stockholm

characteristic: geographic location (region and locality)

text: virus

characteristic: investigation type

text: wastewater metagenome

text: not provided

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W24 Stockholm Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

text: public

text: not provided

text: Untreated wastewater was collected in Malmö at Sjölunda reningsverk on 2022-06-13 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-KTH. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Malmö

text: virus

text: Sjölunda reningsverk

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: organism

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W24 Malmö Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Stockholm at Käppalaverket, Bromma reningsverk and Henriksdal reningsverk on 2022-06-20 over 24 hours using flow compensated samplers. The samples were then combined flow-proportionally into a composite sample for the city. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-KTH. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

text: Stockholm

characteristic: geographic location (region and locality)

text: virus

characteristic: investigation type

text: wastewater metagenome

text: not provided

text: 24 hour period, using a flow compensated sampler at each site, flow-proportionally combined into a single sample

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W25 Stockholm Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

text: public

text: not provided

text: Untreated wastewater was collected in Uppsala at Kungsängsverket over 1 week leading up to 2022-05-23. Daily samples were collected over 24 hours using a flow compensated sampler then combined flow-proportionally into a composite weekly sample. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Uppsala

text: virus

text: Kungsängsverket

characteristic: name of the sampling site

text: Ion AmpliSeq SARS-CoV-2 Insight Research Assay with SuperScript™ VILO™ cDNA Synthesis Kit using Ion Chef™ (Thermo Fisher, # A51306, # 11754050, # MAN0019277, Rev B.0, pp. 3-4, # MAN0024915 Rev B.0, Ch 4, pp. 42-43)

characteristic: nucleic acid amplification

text: TNA concentration using Maxwell RSC Enviro Total Nucleic Acid Kit (Promega, # AS1831 & # TM663 Rev 4/22)

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: organism

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

text: 1 week period, flow-proportionally combined from daily samples, daily sampling over 24 hour period, using a flow compensated sampler

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W21 Uppsala Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Swedish University of Agricultural Sciences

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Kalmar at Kalmarsundsverket on 2022-06-06 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Kalmar

characteristic: geographic location (region and locality)

text: virus

characteristic: investigation type

text: Kalmarsundsverket

text: wastewater metagenome

text: not provided

text: 24 hour period, using a flow compensated sampler

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: Severe acute respiratory syndrome coronavirus 2

text: 2022-W23 Kalmar Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: not provided

text: Untreated wastewater was collected in Umeå at Öns reningsverk on 2022-06-06 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

text: Umeå

text: virus

text: Öns reningsverk

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: organism

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W23 Umeå Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Kalmar at Kalmarsundsverket on 2022-06-13 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Kalmar

characteristic: geographic location (region and locality)

text: virus

characteristic: investigation type

text: Kalmarsundsverket

text: wastewater metagenome

text: not provided

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W24 Kalmar Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: not provided

text: Untreated wastewater was collected in Umeå at Öns reningsverk on 2022-06-13 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

text: Umeå

text: virus

text: Öns reningsverk

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: organism

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W24 Umeå Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Uppsala at Kungsängsverket over 1 week leading up to 2022-06-13. Daily samples were collected over 24 hours using a flow compensated sampler then combined flow-proportionally into a composite weekly sample. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Uppsala

characteristic: geographic location (region and locality)

text: virus

characteristic: investigation type

text: Kungsängsverket

text: wastewater metagenome

text: not provided

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W24 Uppsala Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: not provided

text: Untreated wastewater was collected in Kalmar at Kalmarsundsverket on 2022-06-20 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

text: Kalmar

text: virus

text: Kalmarsundsverket

characteristic: name of the sampling site

text: Ion AmpliSeq SARS-CoV-2 Insight Research Assay with SuperScript™ VILO™ cDNA Synthesis Kit using Ion Chef™ (Thermo Fisher, # A51306, # 11754050, # MAN0019277, Rev B.0, pp. 3-4, # MAN0024915 Rev B.0, Ch 4, pp. 42-43)

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: organism

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W25 Kalmar Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Uppsala at Kungsängsverket over 1 week leading up to 2022-06-20. Daily samples were collected over 24 hours using a flow compensated sampler then combined flow-proportionally into a composite weekly sample. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Uppsala

characteristic: geographic location (region and locality)

text: virus

characteristic: investigation type

text: Kungsängsverket

text: TNA concentration using Maxwell RSC Enviro Total Nucleic Acid Kit (Promega, # AS1831 & # TM663 Rev 4/22)

text: wastewater metagenome

text: not provided

text: 1 week period, flow-proportionally combined from daily samples, daily sampling over 24 hour period, using a flow compensated sampler

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W25 Uppsala Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Oncologica UK Ltd

text: public

tag: Namespace:Oncologica UK Ltd

text: COVID-19 Genomics UK Consortium

characteristic: broker name

text: Oncologica UK Ltd

characteristic: collecting institution

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

text: England

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: Severe acute respiratory syndrome coronavirus 2

characteristic: organism

text: active surveillance in response to outbreak

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

characteristic: title

text: not provided

characteristic: virus identifier


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: Yourgene Health

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Yourgene Health

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: Yourgene Health

characteristic: collection date

text: not provided

text: not provided

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

text: not provided

text: not provided


characteristic: ENA-LAST-UPDATE

text: Swedish University of Agricultural Sciences

text: public

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

text: Untreated wastewater was collected in Kalmar at Kalmarsundsverket on 2022-09-05 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Kalmar

text: virus

characteristic: investigation type

text: Kalmarsundsverket

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: organism

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

text: 24 hour period, using a flow compensated sampler

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W36 Kalmar Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Umeå at Öns reningsverk on 2022-09-05 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Umeå

characteristic: geographic location (region and locality)

text: virus

text: Öns reningsverk

text: wastewater metagenome

text: not provided

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W36 Umeå Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: not provided

text: Untreated wastewater was collected in Örebro at Skebäcks reningsverk on 2022-09-05 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

text: Örebro

text: virus

characteristic: investigation type

text: Skebäcks reningsverk

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: organism

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W36 Örebro Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Uppsala at Kungsängsverket over 1 week leading up to 2022-09-05. Daily samples were collected over 24 hours using a flow compensated sampler then combined flow-proportionally into a composite weekly sample. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Uppsala

characteristic: geographic location (region and locality)

text: virus

text: Kungsängsverket

text: wastewater metagenome

text: not provided

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W36 Uppsala Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: not provided

text: Untreated wastewater was collected in Kalmar at Kalmarsundsverket on 2022-08-01 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

text: Kalmar

text: virus

characteristic: investigation type

text: Kalmarsundsverket

characteristic: name of the sampling site

text: Ion AmpliSeq SARS-CoV-2 Insight Research Assay with SuperScript™ VILO™ cDNA Synthesis Kit using Ion Chef™ (Thermo Fisher, # A51306, # 11754050, # MAN0019277, Rev B.0, pp. 3-4, # MAN0024915 Rev B.0, Ch 4, pp. 42-43)

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: organism

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W31 Kalmar Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Umeå at Öns reningsverk on 2022-08-01 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Umeå

characteristic: geographic location (region and locality)

text: virus

text: Öns reningsverk

text: TNA concentration using Maxwell RSC Enviro Total Nucleic Acid Kit (Promega, # AS1831 & # TM663 Rev 4/22)

text: wastewater metagenome

text: not provided

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W31 Umeå Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: not provided

text: Untreated wastewater was collected in Örebro at Skebäcks reningsverk on 2022-08-01 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

text: Örebro

text: virus

characteristic: investigation type

text: Skebäcks reningsverk

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: organism

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

text: Severe acute respiratory syndrome coronavirus 2

characteristic: surveillance target (scientific name)

text: 2022-W31 Örebro Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Swedish University of Agricultural Sciences

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Uppsala at Kungsängsverket over 1 week leading up to 2022-08-01. Daily samples were collected over 24 hours using a flow compensated sampler then combined flow-proportionally into a composite weekly sample. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Uppsala

characteristic: geographic location (region and locality)

text: virus

text: Kungsängsverket

text: wastewater metagenome

text: not provided

text: 1 week period, flow-proportionally combined from daily samples, daily sampling over 24 hour period, using a flow compensated sampler

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W31 Uppsala Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: not provided

text: Untreated wastewater was collected in Kalmar at Kalmarsundsverket on 2022-07-18 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

text: Kalmar

text: virus

characteristic: investigation type

text: Kalmarsundsverket

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: organism

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

text: 24 hour period, using a flow compensated sampler

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W29 Kalmar Amplified SARS-CoV-2 RNA from wastewater


COG-UK/DHSC-CYNSZDE

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Oncologica UK Ltd

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/DHSC-CYNSZDE

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

characteristic: broker name

text: Oncologica UK Ltd

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: active surveillance in response to outbreak

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: COG-UK/DHSC-CYNSZDE

characteristic: title

text: not provided

characteristic: virus identifier


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

text: Untreated wastewater was collected in Umeå at Öns reningsverk on 2022-07-18 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Umeå

text: virus

characteristic: investigation type

text: Öns reningsverk

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: organism

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W29 Umeå Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Örebro at Skebäcks reningsverk on 2022-07-18 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Örebro

characteristic: geographic location (region and locality)

text: virus

text: Skebäcks reningsverk

text: Ion AmpliSeq SARS-CoV-2 Insight Research Assay with SuperScript™ VILO™ cDNA Synthesis Kit using Ion Chef™ (Thermo Fisher, # A51306, # 11754050, # MAN0019277, Rev B.0, pp. 3-4, # MAN0024915 Rev B.0, Ch 4, pp. 42-43)

text: wastewater metagenome

text: not provided

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W29 Örebro Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: not provided

text: Untreated wastewater was collected in Uppsala at Kungsängsverket over 1 week leading up to 2022-07-18. Daily samples were collected over 24 hours using a flow compensated sampler then combined flow-proportionally into a composite weekly sample. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

text: Uppsala

text: virus

characteristic: investigation type

text: Kungsängsverket

characteristic: name of the sampling site

characteristic: nucleic acid amplification

text: TNA concentration using Maxwell RSC Enviro Total Nucleic Acid Kit (Promega, # AS1831 & # TM663 Rev 4/22)

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: organism

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W29 Uppsala Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Kalmar at Kalmarsundsverket on 2022-07-04 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Kalmar

characteristic: geographic location (region and locality)

text: virus

text: Kalmarsundsverket

text: wastewater metagenome

text: not provided

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: Severe acute respiratory syndrome coronavirus 2

text: 2022-W27 Kalmar Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Swedish University of Agricultural Sciences

text: public

text: not provided

text: Untreated wastewater was collected in Umeå at Öns reningsverk on 2022-07-04 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

text: Umeå

text: virus

characteristic: investigation type

text: Öns reningsverk

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: organism

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W27 Umeå Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Örebro at Skebäcks reningsverk on 2022-07-04 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Örebro

characteristic: geographic location (region and locality)

text: virus

text: Skebäcks reningsverk

text: wastewater metagenome

text: not provided

text: 24 hour period, using a flow compensated sampler

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W27 Örebro Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: not provided

text: Untreated wastewater was collected in Uppsala at Kungsängsverket over 1 week leading up to 2022-07-04. Daily samples were collected over 24 hours using a flow compensated sampler then combined flow-proportionally into a composite weekly sample. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

text: Uppsala

text: virus

characteristic: investigation type

text: Kungsängsverket

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: organism

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

text: 1 week period, flow-proportionally combined from daily samples, daily sampling over 24 hour period, using a flow compensated sampler

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W27 Uppsala Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Umeå at Öns reningsverk on 2022-08-22 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Umeå

characteristic: geographic location (region and locality)

text: virus

text: Öns reningsverk

text: Ion AmpliSeq SARS-CoV-2 Insight Research Assay with SuperScript™ VILO™ cDNA Synthesis Kit using Ion Chef™ (Thermo Fisher, # A51306, # 11754050, # MAN0019277, Rev B.0, pp. 3-4, # MAN0024915 Rev B.0, Ch 4, pp. 42-43)

text: wastewater metagenome

text: not provided

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W34 Umeå Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: not provided

text: Untreated wastewater was collected in Örebro at Skebäcks reningsverk on 2022-08-22 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

text: Örebro

text: virus

characteristic: investigation type

text: Skebäcks reningsverk

characteristic: name of the sampling site

characteristic: nucleic acid amplification

text: TNA concentration using Maxwell RSC Enviro Total Nucleic Acid Kit (Promega, # AS1831 & # TM663 Rev 4/22)

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: organism

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W34 Örebro Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Kalmar at Kalmarsundsverket on 2022-08-22 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Kalmar

characteristic: geographic location (region and locality)

text: virus

text: Kalmarsundsverket

text: wastewater metagenome

text: not provided

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: Severe acute respiratory syndrome coronavirus 2

text: 2022-W34 Kalmar Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Swedish University of Agricultural Sciences

text: public

text: not provided

text: Untreated wastewater was collected in Uppsala at Kungsängsverket over 1 week leading up to 2022-08-22. Daily samples were collected over 24 hours using a flow compensated sampler then combined flow-proportionally into a composite weekly sample. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

text: Uppsala

text: virus

characteristic: investigation type

text: Kungsängsverket

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: organism

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W34 Uppsala Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Kalmar at Kalmarsundsverket on 2022-05-30 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Kalmar

characteristic: geographic location (region and locality)

text: virus

text: Kalmarsundsverket

text: wastewater metagenome

text: not provided

text: 24 hour period, using a flow compensated sampler

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W22 Kalmar Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: not provided

text: Untreated wastewater was collected in Stockholm at Käppalaverket, Bromma reningsverk and Henriksdal reningsverk on 2022-07-04 over 24 hours using flow compensated samplers. The samples were then combined flow-proportionally into a composite sample for the city. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-KTH. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

text: Stockholm

text: virus

characteristic: investigation type

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: organism

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

text: 24 hour period, using a flow compensated sampler at each site, flow-proportionally combined into a single sample

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W27 Stockholm Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Oncologica UK Ltd

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

characteristic: broker name

text: Oncologica UK Ltd

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: active surveillance in response to outbreak

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

characteristic: title

text: not provided

characteristic: virus identifier


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

text: Untreated wastewater was collected in Malmö at Sjölunda reningsverk on 2022-07-04 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-KTH. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Malmö

text: virus

characteristic: investigation type

text: Sjölunda reningsverk

characteristic: name of the sampling site

text: Ion AmpliSeq SARS-CoV-2 Insight Research Assay with SuperScript™ VILO™ cDNA Synthesis Kit using Ion Chef™ (Thermo Fisher, # A51306, # 11754050, # MAN0019277, Rev B.0, pp. 3-4, # MAN0024915 Rev B.0, Ch 4, pp. 42-43)

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: organism

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W27 Malmö Amplified SARS-CoV-2 RNA from wastewater