characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: not provided

text: Untreated wastewater was collected in Stockholm at Käppalaverket, Bromma reningsverk and Henriksdal reningsverk on 2022-09-26 over 24 hours using flow compensated samplers. The samples were then combined flow-proportionally into a composite sample for the city. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-KTH. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

text: Stockholm

text: virus

characteristic: investigation type

characteristic: name of the sampling site

text: Ion AmpliSeq SARS-CoV-2 Insight Research Assay with SuperScript™ VILO™ cDNA Synthesis Kit using Ion Chef™ (Thermo Fisher, # A51306, # 11754050, # MAN0019277, Rev B.0, pp. 3-4, # MAN0024915 Rev B.0, Ch 4, pp. 42-43)

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W39 Stockholm Amplified SARS-CoV-2 RNA from wastewater


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Swedish University of Agricultural Sciences

characteristic: Submitter Id

unit: L

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Malmö at Sjölunda reningsverk on 2022-09-26 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-KTH. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Malmö

characteristic: geographic location (region and locality)

text: virus

text: Sjölunda reningsverk

text: TNA concentration using Maxwell RSC Enviro Total Nucleic Acid Kit (Promega, # AS1831 & # TM663 Rev 4/22)

text: wastewater metagenome

characteristic: organism

unit: day

text: not provided

text: 24 hour period, using a flow compensated sampler

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: Severe acute respiratory syndrome coronavirus 2

text: 2022-W39 Malmö Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Oncologica UK Ltd

text: public

tag: Namespace:Oncologica UK Ltd

text: COVID-19 Genomics UK Consortium

characteristic: broker name

text: Oncologica UK Ltd

characteristic: collecting institution

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

text: England

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: active surveillance in response to outbreak

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: not provided

characteristic: virus identifier


COG-UK/DHSC-CYYDEAI

characteristic: ENA-CHECKLIST

text: Yourgene Health

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/DHSC-CYYDEAI

tag: Namespace:Yourgene Health

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: Yourgene Health

characteristic: collection date

text: not provided

text: not provided

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: organism

text: not provided

text: COG-UK/DHSC-CYYDEAI

characteristic: title

text: not provided


COG-UK/DHSC-CYYOCND

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Yourgene Health

text: public

text: COG-UK/DHSC-CYYOCND

text: COVID-19 Genomics UK Consortium

characteristic: broker name

text: Yourgene Health

characteristic: collecting institution

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

text: England

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

text: not provided

text: COG-UK/DHSC-CYYOCND

text: not provided


COG-UK/DHSC-CYBCUUB

characteristic: ENA-CHECKLIST

text: Oncologica UK Ltd

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/DHSC-CYBCUUB

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: Oncologica UK Ltd

characteristic: collection date

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: COG-UK/DHSC-CYBCUUB

text: not provided

characteristic: virus identifier


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Swedish University of Agricultural Sciences

text: public

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

text: Untreated wastewater was collected in Uppsala at Kungsängsverket over 1 week leading up to 2022-10-03. Daily samples were collected over 24 hours using a flow compensated sampler then combined flow-proportionally into a composite weekly sample. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Uppsala

characteristic: geographic location (region and locality)

text: virus

characteristic: investigation type

text: Kungsängsverket

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: organism

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

text: 1 week period, flow-proportionally combined from daily samples, daily sampling over 24 hour period, using a flow compensated sampler

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W40 Uppsala Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


COG-UK/DHSC-CYNZSUG

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Oncologica UK Ltd

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/DHSC-CYNZSUG

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

characteristic: broker name

text: Oncologica UK Ltd

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: COG-UK/DHSC-CYNZSUG

text: not provided

characteristic: virus identifier


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

text: Untreated wastewater was collected in Örebro at Skebäcks reningsverk on 2022-10-03 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Örebro

text: virus

characteristic: investigation type

text: Skebäcks reningsverk

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: organism

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W40 Örebro Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Umeå at Öns reningsverk on 2022-10-03 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Umeå

characteristic: geographic location (region and locality)

text: virus

characteristic: investigation type

text: Öns reningsverk

text: wastewater metagenome

text: not provided

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W40 Umeå Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: not provided

text: Untreated wastewater was collected in Kalmar at Kalmarsundsverket on 2022-10-03 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

text: Kalmar

text: virus

text: Kalmarsundsverket

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: organism

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W40 Kalmar Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Örebro at Skebäcks reningsverk on 2022-10-10 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Örebro

characteristic: geographic location (region and locality)

text: virus

characteristic: investigation type

text: Skebäcks reningsverk

text: Ion AmpliSeq SARS-CoV-2 Insight Research Assay with SuperScript™ VILO™ cDNA Synthesis Kit using Ion Chef™ (Thermo Fisher, # A51306, # 11754050, # MAN0019277, Rev B.0, pp. 3-4, # MAN0024915 Rev B.0, Ch 4, pp. 42-43)

text: wastewater metagenome

text: not provided

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W41 Örebro Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: not provided

text: Untreated wastewater was collected in Umeå at Öns reningsverk on 2022-10-10 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

text: Umeå

text: virus

text: Öns reningsverk

characteristic: name of the sampling site

characteristic: nucleic acid amplification

text: TNA concentration using Maxwell RSC Enviro Total Nucleic Acid Kit (Promega, # AS1831 & # TM663 Rev 4/22)

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: organism

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

text: 24 hour period, using a flow compensated sampler

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W41 Umeå Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Swedish University of Agricultural Sciences

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Kalmar at Kalmarsundsverket on 2022-10-10 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Kalmar

characteristic: geographic location (region and locality)

text: virus

characteristic: investigation type

text: Kalmarsundsverket

text: wastewater metagenome

text: not provided

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: Severe acute respiratory syndrome coronavirus 2

text: 2022-W41 Kalmar Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: not provided

text: Untreated wastewater was collected in Uppsala at Kungsängsverket over 1 week leading up to 2022-10-17. Daily samples were collected over 24 hours using a flow compensated sampler then combined flow-proportionally into a composite weekly sample. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

text: Uppsala

text: virus

text: Kungsängsverket

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: organism

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

text: 1 week period, flow-proportionally combined from daily samples, daily sampling over 24 hour period, using a flow compensated sampler

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W42 Uppsala Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Örebro at Skebäcks reningsverk on 2022-10-17 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Örebro

characteristic: geographic location (region and locality)

text: virus

characteristic: investigation type

text: Skebäcks reningsverk

text: wastewater metagenome

text: not provided

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W42 Örebro Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: not provided

text: Untreated wastewater was collected in Umeå at Öns reningsverk on 2022-10-17 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

text: Umeå

text: virus

text: Öns reningsverk

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: organism

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W42 Umeå Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Kalmar at Kalmarsundsverket on 2022-10-17 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Kalmar

characteristic: geographic location (region and locality)

text: virus

characteristic: investigation type

text: Kalmarsundsverket

text: Ion AmpliSeq SARS-CoV-2 Insight Research Assay with SuperScript™ VILO™ cDNA Synthesis Kit using Ion Chef™ (Thermo Fisher, # A51306, # 11754050, # MAN0019277, Rev B.0, pp. 3-4, # MAN0024915 Rev B.0, Ch 4, pp. 42-43)

text: wastewater metagenome

text: not provided

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W42 Kalmar Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: not provided

text: Untreated wastewater was collected in Jönköping at Simsholmens avloppsreningsverk on 2022-10-17 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

text: Jönköping

text: virus

text: Simsholmens avloppsreningsverk

characteristic: name of the sampling site

characteristic: nucleic acid amplification

text: TNA concentration using Maxwell RSC Enviro Total Nucleic Acid Kit (Promega, # AS1831 & # TM663 Rev 4/22)

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: organism

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W42 Jönköping Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Swedish University of Agricultural Sciences

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Kalmar at Kalmarsundsverket on 2022-08-15 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Kalmar

characteristic: geographic location (region and locality)

text: virus

characteristic: investigation type

text: Kalmarsundsverket

text: wastewater metagenome

text: not provided

text: 24 hour period, using a flow compensated sampler

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W33 Kalmar Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: not provided

text: Untreated wastewater was collected in Uppsala at Kungsängsverket over 1 week leading up to 2022-08-29. Daily samples were collected over 24 hours using a flow compensated sampler then combined flow-proportionally into a composite weekly sample. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

text: Uppsala

text: virus

text: Kungsängsverket

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: organism

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

text: Severe acute respiratory syndrome coronavirus 2

characteristic: surveillance target (scientific name)

text: 2022-W35 Uppsala Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Umeå at Öns reningsverk on 2022-08-29 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Umeå

characteristic: geographic location (region and locality)

text: virus

characteristic: investigation type

text: Öns reningsverk

text: wastewater metagenome

text: not provided

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W35 Umeå Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: not provided

text: Untreated wastewater was collected in Kalmar at Kalmarsundsverket on 2022-08-29 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

text: Kalmar

text: virus

text: Kalmarsundsverket

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: organism

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W35 Kalmar Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Umeå at Öns reningsverk on 2022-08-15 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Umeå

characteristic: geographic location (region and locality)

text: virus

characteristic: investigation type

text: Öns reningsverk

text: wastewater metagenome

text: not provided

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W33 Umeå Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: not provided

text: Untreated wastewater was collected in Örebro at Skebäcks reningsverk on 2022-08-15 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

text: Örebro

text: virus

text: Skebäcks reningsverk

characteristic: name of the sampling site

text: Ion AmpliSeq SARS-CoV-2 Insight Research Assay with SuperScript™ VILO™ cDNA Synthesis Kit using Ion Chef™ (Thermo Fisher, # A51306, # 11754050, # MAN0019277, Rev B.0, pp. 3-4, # MAN0024915 Rev B.0, Ch 4, pp. 42-43)

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: organism

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W33 Örebro Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Malmö at Sjölunda reningsverk on 2022-07-11 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-KTH. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Malmö

characteristic: geographic location (region and locality)

text: virus

characteristic: investigation type

text: Sjölunda reningsverk

text: TNA concentration using Maxwell RSC Enviro Total Nucleic Acid Kit (Promega, # AS1831 & # TM663 Rev 4/22)

text: wastewater metagenome

text: not provided

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W28 Malmö Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Swedish University of Agricultural Sciences

text: public

text: not provided

text: Untreated wastewater was collected in Malmö at Sjölunda reningsverk on 2022-08-29 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-KTH. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

text: Malmö

text: virus

text: Sjölunda reningsverk

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: organism

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

text: 24 hour period, using a flow compensated sampler

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W35 Malmö Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Stockholm at Käppalaverket, Bromma reningsverk and Henriksdal reningsverk on 2022-08-08 over 24 hours using flow compensated samplers. The samples were then combined flow-proportionally into a composite sample for the city. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-KTH. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

text: Stockholm

characteristic: geographic location (region and locality)

text: virus

characteristic: investigation type

text: wastewater metagenome

text: not provided

text: 24 hour period, using a flow compensated sampler at each site, flow-proportionally combined into a single sample

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: Severe acute respiratory syndrome coronavirus 2

text: 2022-W32 Stockholm Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

text: public

text: not provided

text: Untreated wastewater was collected in Malmö at Sjölunda reningsverk on 2022-08-08 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-KTH. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Malmö

text: virus

text: Sjölunda reningsverk

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: organism

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W32 Malmö Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Stockholm at Käppalaverket, Bromma reningsverk and Henriksdal reningsverk on 2022-08-22 over 24 hours using flow compensated samplers. The samples were then combined flow-proportionally into a composite sample for the city. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-KTH. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

text: Stockholm

characteristic: geographic location (region and locality)

text: virus

characteristic: investigation type

text: wastewater metagenome

text: not provided

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W34 Stockholm Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

text: public

text: not provided

text: Untreated wastewater was collected in Malmö at Sjölunda reningsverk on 2022-08-22 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-KTH. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Malmö

text: virus

text: Sjölunda reningsverk

characteristic: name of the sampling site

text: Ion AmpliSeq SARS-CoV-2 Insight Research Assay with SuperScript™ VILO™ cDNA Synthesis Kit using Ion Chef™ (Thermo Fisher, # A51306, # 11754050, # MAN0019277, Rev B.0, pp. 3-4, # MAN0024915 Rev B.0, Ch 4, pp. 42-43)

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: organism

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W34 Malmö Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Stockholm at Käppalaverket, Bromma reningsverk and Henriksdal reningsverk on 2022-10-03 over 24 hours using flow compensated samplers. The samples were then combined flow-proportionally into a composite sample for the city. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-KTH. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

text: Stockholm

characteristic: geographic location (region and locality)

text: virus

characteristic: investigation type

text: TNA concentration using Maxwell RSC Enviro Total Nucleic Acid Kit (Promega, # AS1831 & # TM663 Rev 4/22)

text: wastewater metagenome

text: not provided

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W40 Stockholm Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

text: Swedish University of Agricultural Sciences

text: public

text: not provided

text: Untreated wastewater was collected in Malmö at Sjölunda reningsverk on 2022-10-03 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-KTH. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Malmö

text: virus

text: Sjölunda reningsverk

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: organism

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W40 Malmö Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Stockholm at Käppalaverket, Bromma reningsverk and Henriksdal reningsverk on 2022-10-10 over 24 hours using flow compensated samplers. The samples were then combined flow-proportionally into a composite sample for the city. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-KTH. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

text: Stockholm

characteristic: geographic location (region and locality)

text: virus

characteristic: investigation type

text: wastewater metagenome

text: not provided

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W41 Stockholm Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

text: public

text: not provided

text: Untreated wastewater was collected in Malmö at Sjölunda reningsverk on 2022-10-10 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-KTH. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Malmö

text: virus

text: Sjölunda reningsverk

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: organism

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

text: 24 hour period, using a flow compensated sampler

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

text: Severe acute respiratory syndrome coronavirus 2

characteristic: surveillance target (scientific name)

text: 2022-W41 Malmö Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Stockholm at Käppalaverket, Bromma reningsverk and Henriksdal reningsverk on 2022-10-17 over 24 hours using flow compensated samplers. The samples were then combined flow-proportionally into a composite sample for the city. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-KTH. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

text: Stockholm

characteristic: geographic location (region and locality)

text: virus

characteristic: investigation type

text: wastewater metagenome

text: not provided

text: 24 hour period, using a flow compensated sampler at each site, flow-proportionally combined into a single sample

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W42 Stockholm Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

text: public

text: not provided

text: Untreated wastewater was collected in Malmö at Sjölunda reningsverk on 2022-10-17 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-KTH. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Malmö

text: virus

text: Sjölunda reningsverk

characteristic: name of the sampling site

text: Ion AmpliSeq SARS-CoV-2 Insight Research Assay with SuperScript™ VILO™ cDNA Synthesis Kit using Ion Chef™ (Thermo Fisher, # A51306, # 11754050, # MAN0019277, Rev B.0, pp. 3-4, # MAN0024915 Rev B.0, Ch 4, pp. 42-43)

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: organism

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W42 Malmö Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


COG-UK/DHSC-CYDTYCS

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Oncologica UK Ltd

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/DHSC-CYDTYCS

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

characteristic: broker name

text: Oncologica UK Ltd

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: active surveillance in response to outbreak

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: COG-UK/DHSC-CYDTYCS

text: not provided

characteristic: virus identifier


COG-UK/DHSC-CYDCUQK

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Oncologica UK Ltd

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/DHSC-CYDCUQK

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: Oncologica UK Ltd

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: organism

text: not provided

text: COG-UK/DHSC-CYDCUQK

characteristic: title

text: not provided


text: Oncologica UK Ltd

text: public

text: COVID-19 Genomics UK Consortium

characteristic: broker name

text: Oncologica UK Ltd

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

text: not provided

text: not provided


COG-UK/DHSC-CYDETZB

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Oncologica UK Ltd

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: COG-UK/DHSC-CYDETZB

text: COVID-19 Genomics UK Consortium

text: Oncologica UK Ltd

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: COG-UK/DHSC-CYDETZB

text: not provided

characteristic: virus identifier


COG-UK/DHSC-CYNYFFD

text: Yourgene Health

text: public

characteristic: SRA accession

text: COG-UK/DHSC-CYNYFFD

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

characteristic: broker name

text: Yourgene Health

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: organism

text: not provided

text: COG-UK/DHSC-CYNYFFD

characteristic: title

text: not provided


COG-UK/DHSC-CYBKRHN

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Oncologica UK Ltd

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: COG-UK/DHSC-CYBKRHN

text: COVID-19 Genomics UK Consortium

text: Oncologica UK Ltd

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

text: not provided

text: COG-UK/DHSC-CYBKRHN

text: not provided


COG-UK/DHSC-CYBOQKO

text: Yourgene Health

text: public

characteristic: SRA accession

text: COG-UK/DHSC-CYBOQKO

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

characteristic: broker name

text: Yourgene Health

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: COG-UK/DHSC-CYBOQKO

text: not provided

characteristic: virus identifier


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Yourgene Health

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: COVID-19 Genomics UK Consortium

text: Yourgene Health

text: not provided

text: not provided

text: United Kingdom

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: organism

text: not provided

characteristic: title

text: not provided


text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Örebro at Skebäcks reningsverk on 2022-08-08 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Örebro

text: virus

characteristic: investigation type

text: Skebäcks reningsverk

characteristic: name of the sampling site

characteristic: nucleic acid amplification

text: TNA concentration using Maxwell RSC Enviro Total Nucleic Acid Kit (Promega, # AS1831 & # TM663 Rev 4/22)

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W32 Örebro Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Swedish University of Agricultural Sciences

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Umeå at Öns reningsverk on 2022-08-08 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Umeå

characteristic: geographic location (region and locality)

text: virus

text: Öns reningsverk

text: wastewater metagenome

characteristic: organism

text: not provided

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W32 Umeå Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: not provided

text: Untreated wastewater was collected in Uppsala at Kungsängsverket over 1 week leading up to 2022-08-08. Daily samples were collected over 24 hours using a flow compensated sampler then combined flow-proportionally into a composite weekly sample. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

characteristic: geographic location (longitude)

text: Uppsala

text: virus

characteristic: investigation type

text: Kungsängsverket

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

text: 1 week period, flow-proportionally combined from daily samples, daily sampling over 24 hour period, using a flow compensated sampler

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W32 Uppsala Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Kalmar at Kalmarsundsverket on 2022-10-24 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Kalmar

characteristic: geographic location (region and locality)

text: virus

text: Kalmarsundsverket

text: wastewater metagenome

characteristic: organism

text: not provided

text: 24 hour period, using a flow compensated sampler

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: Severe acute respiratory syndrome coronavirus 2

text: 2022-W43 Kalmar Amplified SARS-CoV-2 RNA from wastewater

characteristic: title