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text: Sequenced wastewater sample from Central WWTP Saleska dolina 2

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text: Protocols: fresh stool samples were collected from each mice in a collection tube and immediately frozen at -80°. DNA microbial extraction was performed using the QIAamp DNA Stool Mini Kit (Qiagen) starting from 250 µl of each sample according to the manufacturer’s protocol. After assessing DNA concentration and purity, samples were stored at -80° until processing. The V3-V4 hypervariable region of the bacterial 16S rRNA gene was amplified from total DNA according to the Illumina 16S Metagenomic Sequencing Library Preparation instructions, as previously described in Diamanti-Picchianti A, et al (Int J Mol Med ). In summary, the V3-V4 amplicon was obtained by PCR with universal primers, followed by purification. A second PCR was then performed to barcode the libraries using the Illumina dual-index system. Following a second step of purification, libraries were diluted to 4nM and pooled.

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characteristic: Submitter Id

characteristic: description

text: wastewater treatment plant influent

text: wastewater treatment plant

text: wastewater treatment plant influent

text: Slovenia

text: virus

characteristic: organism

text: Illumina

characteristic: title

text: wastewater/sludge


characteristic: Contains RNA extracts (sample codes)

characteristic: Cq (N1)

characteristic: Date of sending/transfer to sequencing location

characteristic: File name

text: National Institute of Biology

text: public

characteristic: No. replicate

characteristic: Sequenced where/how

text: INEXTVIR -20

characteristic: Storage location at NIB

text: wastewater treatment plant influent

characteristic: environment (biome)

text: wastewater treatment plant

characteristic: environment (feature)

text: wastewater treatment plant influent

characteristic: environment (material)

text: Slovenia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: virus

characteristic: investigation type

characteristic: project name

text: Illumina

characteristic: sequencing method

text: wastewater/sludge

characteristic: wastewater/sludge environmental package


characteristic: Contains RNA extracts (sample codes)

characteristic: Cq (N2)

characteristic: DNA concentration (Qubit)

characteristic: Date of amplification (ARTIC)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: National Institute of Biology

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: INEXTVIR -20

characteristic: Submitter Id

characteristic: description

text: wastewater treatment plant influent

text: wastewater treatment plant

text: wastewater treatment plant influent

text: Slovenia

text: virus

characteristic: organism

text: Illumina

characteristic: title

text: wastewater/sludge


characteristic: Contains RNA extracts (sample codes)

characteristic: Cq (N1)

characteristic: Date of sending/transfer to sequencing location

characteristic: File name

text: National Institute of Biology

text: public

characteristic: No. replicate

characteristic: Sequenced where/how

text: INEXTVIR -20

characteristic: Storage location at NIB

text: wastewater treatment plant influent

characteristic: environment (biome)

text: wastewater treatment plant

characteristic: environment (feature)

text: wastewater treatment plant influent

characteristic: environment (material)

text: Slovenia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: virus

characteristic: investigation type

characteristic: project name

text: Illumina

characteristic: sequencing method

text: wastewater/sludge

characteristic: wastewater/sludge environmental package


characteristic: Contains RNA extracts (sample codes)

characteristic: Cq (N2)

characteristic: DNA concentration (Qubit)

characteristic: Date of amplification (ARTIC)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: National Institute of Biology

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: INEXTVIR -20

characteristic: Submitter Id

characteristic: description

text: wastewater treatment plant influent

text: wastewater treatment plant

text: wastewater treatment plant influent

text: Slovenia

text: virus

characteristic: organism

text: Illumina

characteristic: title

text: wastewater/sludge


characteristic: Contains RNA extracts (sample codes)

text: Und.

characteristic: Cq (N1)

characteristic: Date of sending/transfer to sequencing location

characteristic: File name

text: National Institute of Biology

text: public

characteristic: No. replicate

characteristic: Sequenced where/how

text: INEXTVIR -20

characteristic: Storage location at NIB

text: wastewater treatment plant influent

characteristic: environment (biome)

text: wastewater treatment plant

characteristic: environment (feature)

text: wastewater treatment plant influent

characteristic: environment (material)

text: Slovenia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: virus

characteristic: investigation type

characteristic: project name

text: Illumina

characteristic: sequencing method

text: wastewater/sludge

characteristic: wastewater/sludge environmental package


characteristic: Contains RNA extracts (sample codes)

text: Und.

characteristic: Cq (N2)

characteristic: DNA concentration (Qubit)

characteristic: Date of amplification (ARTIC)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: National Institute of Biology

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: INEXTVIR -20

characteristic: Submitter Id

characteristic: description

text: wastewater treatment plant influent

text: wastewater treatment plant

text: wastewater treatment plant influent

text: Slovenia

text: virus

characteristic: organism

text: Illumina

characteristic: title

text: wastewater/sludge


text: Not available

characteristic: ArrayExpress-PHENOTYPE

text: Plasmodium falciparum

characteristic: ArrayExpress-SPECIES

text: SC

text: public

text: Not available

characteristic: PHENOTYPE

text: malaria parasite P. falciparum

characteristic: common name

text: Plasmodium falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum


characteristic: Contains RNA extracts (sample codes)

characteristic: Cq (N1)

characteristic: Cq (N2)

characteristic: DNA concentration (Qubit)

characteristic: Date of amplification (ARTIC)

characteristic: Date of sending/transfer to sequencing location

characteristic: ENA-CHECKLIST

characteristic: File name

text: National Institute of Biology

text: public

characteristic: No. replicate

characteristic: Sequenced where/how

text: INEXTVIR -20

characteristic: Storage location at NIB

text: wastewater treatment plant influent

characteristic: environment (biome)

text: wastewater treatment plant

characteristic: environment (feature)

text: wastewater treatment plant influent

characteristic: environment (material)

text: Slovenia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: virus

characteristic: investigation type

characteristic: project name

text: Illumina

characteristic: sequencing method

text: wastewater/sludge

characteristic: wastewater/sludge environmental package


characteristic: Contains RNA extracts (sample codes)

characteristic: Cq (N1)

characteristic: Cq (N2)

characteristic: DNA concentration (Qubit)

characteristic: Date of amplification (ARTIC)

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: National Institute of Biology

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: INEXTVIR -20

characteristic: Submitter Id

characteristic: description

text: wastewater treatment plant influent

text: wastewater treatment plant

text: wastewater treatment plant influent

text: Slovenia

text: virus

characteristic: organism

text: Illumina

characteristic: title

text: wastewater/sludge


E-MTAB-11781:Sample 1

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-11781:Sample 1

tag: Namespace:Institute of Immunobiology, Kantonsspital St. Gallen, St. Gallen, Switzerland

text: 8 to 10

unit: week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: non-hematopoietic cell and hematopoietic cell

text: house mouse

characteristic: common name

text: Protocols: Mice were sacrificed at age 8-10 weeks and lungs were harvested for further stromal cell or immune cell isolation and scRNA sequencing analysis. For cell preparation tissues were cut into small pieces. M-CoV immunization protocol: Mice were infected intranasally with 5 × 104 plaque-forming units (PFU) of M-CoV-A59 of M-CoV-GFP . Lungs were collected at 36 hours after infection for further processing for single cell RNA-sequencing Cell isolation from mouse lungs was performed according to standardized protocols. Mice were euthanized at the indicated time points and immediately perfused with PBS. Lungs were transferred into a 6-well plate containing RPMI 1640 medium (Sigma-Aldrich) supplemented with 4 U/mL elastase (Worthington), 1 U/mL Dispase II (Roche) and 10 μl/mL DNAse I (Applichem) and incubated for 45 minutes at 37°C. Lung tissue was then mechanically disrupted and washed in cold RPMI. Tissues were subject to a second enzymatic digestion in RPMI 1640 medium supplemented with 25 μg/mL Liberase TM (Roche) and 0.25 μg/mL DNAse I and incubated for 30 minutes at 37 °C. Any larger tissue fragments were dissociated mechanically followed by washing of the cell strainer with MACS buffer (PBS, 2% FCS, 2mM EDTA (Sigma-Aldrich)). Stromal and hematopoietic cell fractions were enriched using MACS anti-CD45 microbeads (Miltenyi Biotec). Cell sorting was performed on a BD FACSMelody™ cell sorter using BDChorus software. For discrimination between live and dead cells, Seven-aminoactinomycin D (Sigma-Aldrich) was added prior to sorting. Sorted cells were passed through the 10x Chromium (10x Genomics) system and cDNA libraries were generated according to the manufacturer’s recommendations (Chromium Single Cell 3′ Reagent Kit (version 3 chemistry)). cDNA library generation was performed following the established commercial protocol for Chromium Single Cell 3’ Reagent Kit (v3 Chemistry).

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

characteristic: immunophenotype

text: pool of 4 to 8 individuals

characteristic: individual

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: lung

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

characteristic: strain

text: Sample 1


E-MTAB-11781:Sample 2

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Institute of Immunobiology, Kantonsspital St. Gallen, St. Gallen, Switzerland

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11781:Sample 2

text: 8 to 10

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: pool of 4 to 8 individuals

text: not applicable

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

text: female

text: Sample 2


E-MTAB-11781:Sample 3

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-11781:Sample 3

characteristic: Submitter Id

text: 8 to 10

characteristic: age

text: ArrayExpress

characteristic: broker name

text: non-hematopoietic cell

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: wild type genotype

text: CD45-; GFP-

text: pool of 4 to 8 individuals

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lung

text: Mus musculus

text: female

text: Sample 3

characteristic: title


E-MTAB-11781:Sample 4

text: public

text: E-MTAB-11781:Sample 4

text: 8 to 10

text: ArrayExpress

text: hematopoietic cell

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: CD45+; GFP+

characteristic: immunophenotype

text: pool of 4 to 8 individuals

characteristic: individual

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: lung

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

characteristic: strain

text: Sample 4


E-MTAB-11781:Sample 5

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11781:Sample 5

text: 8 to 10

text: ArrayExpress

text: non-hematopoietic cell

text: house mouse

text: adult

text: wild type genotype

text: CD45-; GFP-

text: pool of 4 to 8 individuals

text: not applicable

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

text: female

text: Sample 5


E-MTAB-11781:Sample 6

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-11781:Sample 6

characteristic: Submitter Id

text: 8 to 10

characteristic: age

text: ArrayExpress

characteristic: broker name

text: hematopoietic cell

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: wild type genotype

text: CD45+; GFP+

text: pool of 4 to 8 individuals

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lung

text: Mus musculus

text: female

text: Sample 6

characteristic: title


E-MTAB-11781:Sample 7

text: public

text: E-MTAB-11781:Sample 7

text: 8 to 10

text: ArrayExpress

text: non-hematopoietic cell

text: house mouse

text: adult

characteristic: developmental stage

text: C57BL/6N-Tg(LysM-Cre) x R26-EYFP x Ifnar(fl/fl)

characteristic: genotype

text: CD45-; GFP-

characteristic: immunophenotype

text: pool of 4 to 8 individuals

characteristic: individual

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: lung

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

characteristic: strain

text: Sample 7


E-MTAB-11781:Sample 8

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11781:Sample 8

text: 8 to 10

text: ArrayExpress

text: hematopoietic cell

text: house mouse

text: adult

text: CD45+; GFP+

text: pool of 4 to 8 individuals

text: not applicable

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

text: female

text: Sample 8


E-MTAB-11781:Sample 9

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-11781:Sample 9

characteristic: Submitter Id

text: 8 to 10

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: pool of 4 to 8 individuals

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lung

text: Mus musculus

text: female

text: Sample 9

characteristic: title


characteristic: Contains RNA extracts (sample codes)

characteristic: Cq (N1)

characteristic: Cq (N2)

characteristic: DNA concentration (Qubit)

characteristic: Date of amplification (ARTIC)

characteristic: Date of sending/transfer to sequencing location

characteristic: Forward file name

text: National Institute of Biology

text: public

characteristic: No. replicate

characteristic: Sequenced where/how

text: INEXTVIR -20

characteristic: Storage location at NIB

text: Sequenced wastewater sample from Central WWTP Koper 1

text: wastewater treatment plant influent

characteristic: environment (biome)

text: wastewater treatment plant

characteristic: environment (feature)

text: wastewater treatment plant influent

characteristic: environment (material)

text: Slovenia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: virus

characteristic: investigation type

characteristic: project name

text: Illumina

characteristic: sequencing method

text: wastewater/sludge

characteristic: wastewater/sludge environmental package


characteristic: Contains RNA extracts (sample codes)

characteristic: Cq (N1)

characteristic: Cq (N2)

characteristic: DNA concentration (Qubit)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: National Institute of Biology

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: INEXTVIR -20

characteristic: Submitter Id

text: Sequenced wastewater sample from Central WWTP Koper 2

characteristic: description

text: wastewater treatment plant influent

text: wastewater treatment plant

text: wastewater treatment plant influent

text: Slovenia

text: virus

characteristic: organism

text: Illumina

characteristic: title

text: wastewater/sludge


characteristic: Contains RNA extracts (sample codes)

characteristic: Date of amplification (ARTIC)

characteristic: Date of sending/transfer to sequencing location

characteristic: Forward file name

text: National Institute of Biology

text: public

characteristic: No. replicate

characteristic: Sequenced where/how

text: INEXTVIR -20

characteristic: Storage location at NIB

text: Sequenced wastewater sample from Central WWTP Kranj 1

text: wastewater treatment plant influent

characteristic: environment (biome)

text: wastewater treatment plant

characteristic: environment (feature)

text: wastewater treatment plant influent

characteristic: environment (material)

text: Slovenia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: virus

characteristic: investigation type

characteristic: project name

text: Illumina

characteristic: sequencing method

text: wastewater/sludge

characteristic: wastewater/sludge environmental package


characteristic: Contains RNA extracts (sample codes)

characteristic: Cq (N1)

characteristic: Cq (N2)

characteristic: DNA concentration (Qubit)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: National Institute of Biology

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: INEXTVIR -20

characteristic: Submitter Id

text: Sequenced wastewater sample from Central WWTP Kranj 2

characteristic: description

text: wastewater treatment plant influent

text: wastewater treatment plant

text: wastewater treatment plant influent

text: Slovenia

text: virus

characteristic: organism

text: Illumina

characteristic: title

text: wastewater/sludge


characteristic: Contains RNA extracts (sample codes)

characteristic: Date of amplification (ARTIC)

characteristic: Date of sending/transfer to sequencing location

characteristic: Forward file name

text: National Institute of Biology

text: public

characteristic: No. replicate

characteristic: Sequenced where/how

text: INEXTVIR -20

characteristic: Storage location at NIB

text: wastewater treatment plant influent

characteristic: environment (biome)

text: wastewater treatment plant

characteristic: environment (feature)

text: wastewater treatment plant influent

characteristic: environment (material)

text: Slovenia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: virus

characteristic: investigation type

characteristic: project name

text: Illumina

characteristic: sequencing method

text: wastewater/sludge

characteristic: wastewater/sludge environmental package


characteristic: Contains RNA extracts (sample codes)

characteristic: Cq (N1)

characteristic: Cq (N2)

characteristic: DNA concentration (Qubit)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: National Institute of Biology

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: INEXTVIR -20

characteristic: Submitter Id

characteristic: description

text: wastewater treatment plant influent

text: wastewater treatment plant

text: wastewater treatment plant influent

text: Slovenia

text: virus

characteristic: organism

text: Illumina

characteristic: title

text: wastewater/sludge


characteristic: Contains RNA extracts (sample codes)

characteristic: Date of amplification (ARTIC)

characteristic: Date of sending/transfer to sequencing location

characteristic: Forward file name

text: National Institute of Biology

text: public

characteristic: No. replicate

characteristic: Sequenced where/how

text: INEXTVIR -20

characteristic: Storage location at NIB

text: Sequenced wastewater sample from Central WWTP Ljubljana 1

text: wastewater treatment plant influent

characteristic: environment (biome)

text: wastewater treatment plant

characteristic: environment (feature)

text: wastewater treatment plant influent

characteristic: environment (material)

text: Slovenia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: virus

characteristic: investigation type

characteristic: project name

text: Illumina

characteristic: sequencing method

text: wastewater/sludge

characteristic: wastewater/sludge environmental package


characteristic: Contains RNA extracts (sample codes)

characteristic: Cq (N1)

characteristic: Cq (N2)

characteristic: DNA concentration (Qubit)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: National Institute of Biology

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: INEXTVIR -20

characteristic: Submitter Id

text: Sequenced wastewater sample from Central WWTP Ljubljana 2

characteristic: description

text: wastewater treatment plant influent

text: wastewater treatment plant

text: wastewater treatment plant influent

text: Slovenia

text: virus

characteristic: organism

text: Illumina

characteristic: title

text: wastewater/sludge


characteristic: Contains RNA extracts (sample codes)

characteristic: Date of amplification (ARTIC)

characteristic: Date of sending/transfer to sequencing location

characteristic: Forward file name

text: National Institute of Biology

text: public

characteristic: No. replicate

characteristic: Sequenced where/how

text: INEXTVIR -20

characteristic: Storage location at NIB

text: Sequenced wastewater sample from Central WWTP Saleska dolina 1

text: wastewater treatment plant influent

characteristic: environment (biome)

text: wastewater treatment plant

characteristic: environment (feature)

text: wastewater treatment plant influent

characteristic: environment (material)

text: Slovenia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: virus

characteristic: investigation type

characteristic: project name

text: Illumina

characteristic: sequencing method

text: wastewater/sludge

characteristic: wastewater/sludge environmental package


characteristic: Contains RNA extracts (sample codes)

characteristic: Cq (N1)

characteristic: Cq (N2)

characteristic: DNA concentration (Qubit)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: National Institute of Biology

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: INEXTVIR -20

characteristic: Submitter Id

characteristic: description

text: wastewater treatment plant influent

text: wastewater treatment plant

text: wastewater treatment plant influent

text: Slovenia

text: virus

characteristic: organism

text: Illumina

characteristic: title

text: wastewater/sludge


characteristic: Contains RNA extracts (sample codes)

characteristic: Date of amplification (ARTIC)

characteristic: Date of sending/transfer to sequencing location

characteristic: Forward file name

text: National Institute of Biology

text: public

characteristic: No. replicate

characteristic: Sequenced where/how

text: INEXTVIR -20

characteristic: Storage location at NIB

text: Sequenced wastewater sample from Central WWTP Maribor 1

text: wastewater treatment plant influent

characteristic: environment (biome)

text: wastewater treatment plant

characteristic: environment (feature)

text: wastewater treatment plant influent

characteristic: environment (material)

text: Slovenia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: virus

characteristic: investigation type

characteristic: project name

text: Illumina

characteristic: sequencing method

text: wastewater/sludge

characteristic: wastewater/sludge environmental package


characteristic: Contains RNA extracts (sample codes)

characteristic: Cq (N1)

characteristic: Cq (N2)

characteristic: DNA concentration (Qubit)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: National Institute of Biology

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: INEXTVIR -20

characteristic: Submitter Id

text: Sequenced wastewater sample from Central WWTP Maribor 2

characteristic: description

text: wastewater treatment plant influent

text: wastewater treatment plant

text: wastewater treatment plant influent

text: Slovenia

text: virus

characteristic: organism

text: Illumina

characteristic: title

text: wastewater/sludge


characteristic: Contains RNA extracts (sample codes)

characteristic: Date of amplification (ARTIC)

characteristic: Date of sending/transfer to sequencing location

characteristic: Forward file name

text: National Institute of Biology

text: public

characteristic: No. replicate

characteristic: Remarks

text: Department of Endocrinology, Diabetes and Metabolism, University Children's Hospital, University Medical Centre Ljubljana

characteristic: Sequenced where/how

text: INEXTVIR -20

characteristic: Storage location at NIB

text: wastewater treatment plant influent

characteristic: environment (biome)

text: wastewater treatment plant

characteristic: environment (feature)

text: wastewater treatment plant influent

characteristic: environment (material)

text: Slovenia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: virus

characteristic: investigation type

characteristic: project name

text: Illumina

characteristic: sequencing method

text: wastewater/sludge

characteristic: wastewater/sludge environmental package


characteristic: Contains RNA extracts (sample codes)

characteristic: Cq (N1)

characteristic: Cq (N2)

characteristic: DNA concentration (Qubit)

characteristic: Date of amplification (ARTIC)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: National Institute of Biology

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: INEXTVIR -20

characteristic: Submitter Id

characteristic: description

text: wastewater treatment plant influent

text: wastewater treatment plant

text: wastewater treatment plant influent

text: Slovenia

text: virus

characteristic: organism

text: Illumina

characteristic: title

text: wastewater/sludge


characteristic: Contains RNA extracts (sample codes)

characteristic: Cq (N1)

characteristic: Date of sending/transfer to sequencing location

characteristic: Forward file name

text: National Institute of Biology

text: public

characteristic: No. replicate

characteristic: Remarks

characteristic: Sequenced where/how

text: INEXTVIR -20

characteristic: Storage location at NIB

text: wastewater treatment plant influent

characteristic: environment (biome)

text: wastewater treatment plant

characteristic: environment (feature)

text: wastewater treatment plant influent

characteristic: environment (material)

text: Slovenia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: virus

characteristic: investigation type

characteristic: project name

text: Illumina

characteristic: sequencing method

text: wastewater/sludge

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