characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: not provided

text: Untreated wastewater was collected in Örebro at Skebäcks reningsverk on 2022-10-24 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

characteristic: geographic location (longitude)

text: Örebro

text: virus

characteristic: investigation type

text: Skebäcks reningsverk

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W43 Örebro Amplified SARS-CoV-2 RNA from wastewater


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Swedish University of Agricultural Sciences

characteristic: Submitter Id

unit: L

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Umeå at Öns reningsverk on 2022-10-24 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

text: Umeå

characteristic: geographic location (region and locality)

text: virus

text: Öns reningsverk

text: Ion AmpliSeq SARS-CoV-2 Insight Research Assay with SuperScript™ VILO™ cDNA Synthesis Kit using Ion Chef™ (Thermo Fisher, # A51306, # 11754050, # MAN0019277, Rev B.0, pp. 3-4, # MAN0024915 Rev B.0, Ch 4, pp. 42-43)

text: TNA concentration using Maxwell RSC Enviro Total Nucleic Acid Kit (Promega, # AS1831 & # TM663 Rev 4/22)

text: wastewater metagenome

characteristic: organism

unit: day

text: not provided

text: 24 hour period, using a flow compensated sampler

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: Severe acute respiratory syndrome coronavirus 2

text: 2022-W43 Umeå Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Swedish University of Agricultural Sciences

text: public

text: not provided

text: Untreated wastewater was collected in Uppsala at Kungsängsverket over 1 week leading up to 2022-10-24. Daily samples were collected over 24 hours using a flow compensated sampler then combined flow-proportionally into a composite weekly sample. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

characteristic: geographic location (longitude)

text: Uppsala

text: virus

characteristic: investigation type

text: Kungsängsverket

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

text: 1 week period, flow-proportionally combined from daily samples, daily sampling over 24 hour period, using a flow compensated sampler

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W43 Uppsala Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Helsingborg at Öresundsverket on 2022-10-24 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Helsingborg

characteristic: geographic location (region and locality)

text: virus

text: Öresundsverket

text: wastewater metagenome

characteristic: organism

text: not provided

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W43 Helsingborg Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: not provided

text: Untreated wastewater was collected in Jönköping at Simsholmens avloppsreningsverk on 2022-10-24 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

characteristic: geographic location (longitude)

text: Jönköping

text: virus

characteristic: investigation type

text: Simsholmens avloppsreningsverk

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W43 Jönköping Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Västerås at Reningsverket Kungsängen on 2022-10-24 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Västerås

characteristic: geographic location (region and locality)

text: virus

text: Reningsverket Kungsängen

text: wastewater metagenome

characteristic: organism

text: not provided

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W43 Västerås Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: not provided

text: Untreated wastewater was collected in Östersund at Gövikens reningsverk on 2022-10-24 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

characteristic: geographic location (longitude)

text: Östersund

text: virus

characteristic: investigation type

text: Gövikens reningsverk

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W43 Östersund Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Kalmar at Kalmarsundsverket on 2022-10-31 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Kalmar

characteristic: geographic location (region and locality)

text: virus

text: Kalmarsundsverket

text: Ion AmpliSeq SARS-CoV-2 Insight Research Assay with SuperScript™ VILO™ cDNA Synthesis Kit using Ion Chef™ (Thermo Fisher, # A51306, # 11754050, # MAN0019277, Rev B.0, pp. 3-4, # MAN0024915 Rev B.0, Ch 4, pp. 42-43)

text: wastewater metagenome

characteristic: organism

text: not provided

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W44 Kalmar Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: not provided

text: Untreated wastewater was collected in Örebro at Skebäcks reningsverk on 2022-10-31 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

characteristic: geographic location (longitude)

text: Örebro

text: virus

characteristic: investigation type

text: Skebäcks reningsverk

characteristic: name of the sampling site

characteristic: nucleic acid amplification

text: TNA concentration using Maxwell RSC Enviro Total Nucleic Acid Kit (Promega, # AS1831 & # TM663 Rev 4/22)

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

text: 24 hour period, using a flow compensated sampler

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

text: Severe acute respiratory syndrome coronavirus 2

characteristic: surveillance target (scientific name)

text: 2022-W44 Örebro Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Swedish University of Agricultural Sciences

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Umeå at Öns reningsverk on 2022-10-31 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Umeå

characteristic: geographic location (region and locality)

text: virus

text: Öns reningsverk

text: wastewater metagenome

characteristic: organism

text: not provided

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W44 Umeå Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: not provided

text: Untreated wastewater was collected in Uppsala at Kungsängsverket over 1 week leading up to 2022-10-31. Daily samples were collected over 24 hours using a flow compensated sampler then combined flow-proportionally into a composite weekly sample. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

characteristic: geographic location (longitude)

text: Uppsala

text: virus

characteristic: investigation type

text: Kungsängsverket

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

text: 1 week period, flow-proportionally combined from daily samples, daily sampling over 24 hour period, using a flow compensated sampler

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W44 Uppsala Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Helsingborg at Öresundsverket on 2022-10-31 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Helsingborg

characteristic: geographic location (region and locality)

text: virus

text: Öresundsverket

text: wastewater metagenome

characteristic: organism

text: not provided

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W44 Helsingborg Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: not provided

text: Untreated wastewater was collected in Jönköping at Simsholmens avloppsreningsverk on 2022-10-31 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

characteristic: geographic location (longitude)

text: Jönköping

text: virus

characteristic: investigation type

text: Simsholmens avloppsreningsverk

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W44 Jönköping Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Västerås at Reningsverket Kungsängen on 2022-10-31 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Västerås

characteristic: geographic location (region and locality)

text: virus

text: Reningsverket Kungsängen

text: Ion AmpliSeq SARS-CoV-2 Insight Research Assay with SuperScript™ VILO™ cDNA Synthesis Kit using Ion Chef™ (Thermo Fisher, # A51306, # 11754050, # MAN0019277, Rev B.0, pp. 3-4, # MAN0024915 Rev B.0, Ch 4, pp. 42-43)

text: wastewater metagenome

characteristic: organism

text: not provided

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W44 Västerås Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: not provided

text: Untreated wastewater was collected in Kalmar at Kalmarsundsverket on 2022-11-07 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

characteristic: geographic location (longitude)

text: Kalmar

text: virus

characteristic: investigation type

text: Kalmarsundsverket

characteristic: name of the sampling site

characteristic: nucleic acid amplification

text: TNA concentration using Maxwell RSC Enviro Total Nucleic Acid Kit (Promega, # AS1831 & # TM663 Rev 4/22)

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W45 Kalmar Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Örebro at Skebäcks reningsverk on 2022-11-07 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Örebro

characteristic: geographic location (region and locality)

text: virus

text: Skebäcks reningsverk

text: wastewater metagenome

characteristic: organism

text: not provided

text: 24 hour period, using a flow compensated sampler

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: Severe acute respiratory syndrome coronavirus 2

text: 2022-W45 Örebro Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Swedish University of Agricultural Sciences

text: public

text: not provided

text: Untreated wastewater was collected in Umeå at Öns reningsverk on 2022-11-07 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

characteristic: geographic location (longitude)

text: Umeå

text: virus

characteristic: investigation type

text: Öns reningsverk

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W45 Umeå Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Uppsala at Kungsängsverket over 1 week leading up to 2022-11-07. Daily samples were collected over 24 hours using a flow compensated sampler then combined flow-proportionally into a composite weekly sample. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Uppsala

characteristic: geographic location (region and locality)

text: virus

text: Kungsängsverket

text: wastewater metagenome

characteristic: organism

text: not provided

text: 1 week period, flow-proportionally combined from daily samples, daily sampling over 24 hour period, using a flow compensated sampler

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W45 Uppsala Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: not provided

text: Untreated wastewater was collected in Helsingborg at Öresundsverket on 2022-11-07 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

characteristic: geographic location (longitude)

text: Helsingborg

text: virus

characteristic: investigation type

text: Öresundsverket

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W45 Helsingborg Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Jönköping at Simsholmens avloppsreningsverk on 2022-11-07 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Jönköping

characteristic: geographic location (region and locality)

text: virus

text: Simsholmens avloppsreningsverk

text: Ion AmpliSeq SARS-CoV-2 Insight Research Assay with SuperScript™ VILO™ cDNA Synthesis Kit using Ion Chef™ (Thermo Fisher, # A51306, # 11754050, # MAN0019277, Rev B.0, pp. 3-4, # MAN0024915 Rev B.0, Ch 4, pp. 42-43)

text: wastewater metagenome

characteristic: organism

text: not provided

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W45 Jönköping Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: not provided

text: Untreated wastewater was collected in Uppsala at Kungsängsverket over 1 week leading up to 2022-10-10. Daily samples were collected over 24 hours using a flow compensated sampler then combined flow-proportionally into a composite weekly sample. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

characteristic: geographic location (longitude)

text: Uppsala

text: virus

characteristic: investigation type

text: Kungsängsverket

characteristic: name of the sampling site

characteristic: nucleic acid amplification

text: TNA concentration using Maxwell RSC Enviro Total Nucleic Acid Kit (Promega, # AS1831 & # TM663 Rev 4/22)

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W41 Uppsala Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Jönköping at Simsholmens avloppsreningsverk on 2022-10-10 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Jönköping

characteristic: geographic location (region and locality)

text: virus

text: Simsholmens avloppsreningsverk

text: wastewater metagenome

characteristic: organism

text: not provided

text: 24 hour period, using a flow compensated sampler

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W41 Jönköping Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Swedish University of Agricultural Sciences

text: public

text: not provided

text: Untreated wastewater was collected in Västerås at Reningsverket Kungsängen on 2022-11-07 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

characteristic: geographic location (longitude)

text: Västerås

text: virus

characteristic: investigation type

text: Reningsverket Kungsängen

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

text: Severe acute respiratory syndrome coronavirus 2

characteristic: surveillance target (scientific name)

text: 2022-W45 Västerås Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Stockholm at Käppalaverket, Bromma reningsverk and Henriksdal reningsverk on 2022-10-24 over 24 hours using flow compensated samplers. The samples were then combined flow-proportionally into a composite sample for the city. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-KTH. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

text: Stockholm

characteristic: geographic location (region and locality)

text: virus

text: wastewater metagenome

characteristic: organism

text: not provided

text: 24 hour period, using a flow compensated sampler at each site, flow-proportionally combined into a single sample

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W43 Stockholm Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: not provided

text: Untreated wastewater was collected in Malmö at Sjölunda reningsverk on 2022-10-24 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-KTH. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Malmö

text: virus

characteristic: investigation type

text: Sjölunda reningsverk

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W43 Malmö Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Stockholm at Käppalaverket, Bromma reningsverk and Henriksdal reningsverk on 2022-10-31 over 24 hours using flow compensated samplers. The samples were then combined flow-proportionally into a composite sample for the city. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-KTH. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

text: Stockholm

characteristic: geographic location (region and locality)

text: virus

text: Ion AmpliSeq SARS-CoV-2 Insight Research Assay with SuperScript™ VILO™ cDNA Synthesis Kit using Ion Chef™ (Thermo Fisher, # A51306, # 11754050, # MAN0019277, Rev B.0, pp. 3-4, # MAN0024915 Rev B.0, Ch 4, pp. 42-43)

text: wastewater metagenome

characteristic: organism

text: not provided

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W44 Stockholm Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: not provided

text: Untreated wastewater was collected in Malmö at Sjölunda reningsverk on 2022-10-31 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-KTH. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Malmö

text: virus

characteristic: investigation type

text: Sjölunda reningsverk

characteristic: name of the sampling site

characteristic: nucleic acid amplification

text: TNA concentration using Maxwell RSC Enviro Total Nucleic Acid Kit (Promega, # AS1831 & # TM663 Rev 4/22)

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W44 Malmö Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Stockholm at Käppalaverket, Bromma reningsverk and Henriksdal reningsverk on 2022-11-07 over 24 hours using flow compensated samplers. The samples were then combined flow-proportionally into a composite sample for the city. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-KTH. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

text: Stockholm

characteristic: geographic location (region and locality)

text: virus

text: wastewater metagenome

characteristic: organism

text: not provided

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W45 Stockholm Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


COG-UK/DHSC-CYBAUUE

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Yourgene Health

text: public

text: COG-UK/DHSC-CYBAUUE

tag: Namespace:Yourgene Health

text: COVID-19 Genomics UK Consortium

characteristic: broker name

text: Yourgene Health

characteristic: collecting institution

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

text: England

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: active surveillance in response to outbreak

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: COG-UK/DHSC-CYBAUUE

text: not provided

characteristic: virus identifier


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Oncologica UK Ltd

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Oncologica UK Ltd

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: Oncologica UK Ltd

characteristic: collection date

text: not provided

text: not provided

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: organism

text: not provided

text: not provided


COG-UK/DHSC-CYYUTWU

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Oncologica UK Ltd

text: public

text: COG-UK/DHSC-CYYUTWU

text: COVID-19 Genomics UK Consortium

characteristic: broker name

text: Oncologica UK Ltd

characteristic: collecting institution

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

text: England

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

text: not provided

text: COG-UK/DHSC-CYYUTWU

characteristic: title

text: not provided


COG-UK/DHSC-CYBUIJF

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Yourgene Health

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/DHSC-CYBUIJF

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: Yourgene Health

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: COG-UK/DHSC-CYBUIJF

text: not provided

characteristic: virus identifier


COG-UK/DHSC-CYNCPSY

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Yourgene Health

text: public

text: COG-UK/DHSC-CYNCPSY

text: COVID-19 Genomics UK Consortium

characteristic: broker name

text: Yourgene Health

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

text: not provided

text: not provided

text: not provided

characteristic: organism

text: not provided

text: COG-UK/DHSC-CYNCPSY

text: not provided


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Swedish University of Agricultural Sciences

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

text: Untreated wastewater was collected in Kalmar at Kalmarsundsverket on 2022-08-08 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Kalmar

text: virus

characteristic: investigation type

text: Kalmarsundsverket

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

text: 24 hour period, using a flow compensated sampler

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W32 Kalmar Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Gävle at Duvbackens reningsverk on 2022-11-14 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Gävle

characteristic: geographic location (region and locality)

text: virus

text: Duvbackens reningsverk

text: wastewater metagenome

characteristic: organism

text: not provided

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: Severe acute respiratory syndrome coronavirus 2

text: 2022-W46 Gävle Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: not provided

text: Untreated wastewater was collected in Helsingborg at Öresundsverket on 2022-11-14 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

text: Helsingborg

text: virus

characteristic: investigation type

text: Öresundsverket

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W46 Helsingborg Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Jönköping at Simsholmens avloppsreningsverk on 2022-11-14 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Jönköping

characteristic: geographic location (region and locality)

text: virus

text: Simsholmens avloppsreningsverk

text: Ion AmpliSeq SARS-CoV-2 Insight Research Assay with SuperScript™ VILO™ cDNA Synthesis Kit using Ion Chef™ (Thermo Fisher, # A51306, # 11754050, # MAN0019277, Rev B.0, pp. 3-4, # MAN0024915 Rev B.0, Ch 4, pp. 42-43)

text: wastewater metagenome

characteristic: organism

text: not provided

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W46 Jönköping Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: not provided

text: Untreated wastewater was collected in Umeå at Öns reningsverk on 2022-11-14 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

text: Umeå

text: virus

characteristic: investigation type

text: Öns reningsverk

characteristic: name of the sampling site

characteristic: nucleic acid amplification

text: TNA concentration using Maxwell RSC Enviro Total Nucleic Acid Kit (Promega, # AS1831 & # TM663 Rev 4/22)

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W46 Umeå Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Uppsala at Kungsängsverket over 1 week leading up to 2022-11-14. Daily samples were collected over 24 hours using a flow compensated sampler then combined flow-proportionally into a composite weekly sample. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Uppsala

characteristic: geographic location (region and locality)

text: virus

text: Kungsängsverket

text: wastewater metagenome

characteristic: organism

text: not provided

text: 1 week period, flow-proportionally combined from daily samples, daily sampling over 24 hour period, using a flow compensated sampler

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W46 Uppsala Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Swedish University of Agricultural Sciences

text: public

text: not provided

text: Untreated wastewater was collected in Västerås at Reningsverket Kungsängen on 2022-11-14 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

text: Västerås

text: virus

characteristic: investigation type

text: Reningsverket Kungsängen

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W46 Västerås Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Östersund at Gövikens reningsverk on 2022-11-14 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Östersund

characteristic: geographic location (region and locality)

text: virus

text: Gövikens reningsverk

text: wastewater metagenome

characteristic: organism

text: not provided

text: 24 hour period, using a flow compensated sampler

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W46 Östersund Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: not provided

text: Untreated wastewater was collected in Gävle at Duvbackens reningsverk on 2022-11-21 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

text: Gävle

text: virus

characteristic: investigation type

text: Duvbackens reningsverk

characteristic: name of the sampling site

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

text: Severe acute respiratory syndrome coronavirus 2

characteristic: surveillance target (scientific name)

text: 2022-W47 Gävle Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Helsingborg at Öresundsverket on 2022-11-21 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Helsingborg

characteristic: geographic location (region and locality)

text: virus

text: Öresundsverket

text: Ion AmpliSeq SARS-CoV-2 Insight Research Assay with SuperScript™ VILO™ cDNA Synthesis Kit using Ion Chef™ (Thermo Fisher, # A51306, # 11754050, # MAN0019277, Rev B.0, pp. 3-4, # MAN0024915 Rev B.0, Ch 4, pp. 42-43)

text: wastewater metagenome

characteristic: organism

text: not provided

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W47 Helsingborg Amplified SARS-CoV-2 RNA from wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: not provided

text: Untreated wastewater was collected in Jönköping at Simsholmens avloppsreningsverk on 2022-11-21 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

text: Sweden

text: Jönköping

text: virus

characteristic: investigation type

text: Simsholmens avloppsreningsverk

characteristic: name of the sampling site

characteristic: nucleic acid amplification

text: TNA concentration using Maxwell RSC Enviro Total Nucleic Acid Kit (Promega, # AS1831 & # TM663 Rev 4/22)

characteristic: nucleic acid extraction

text: wastewater metagenome

characteristic: population size of the catchment area

characteristic: receipt date

characteristic: sample storage duration

text: not provided

characteristic: sample storage location

characteristic: sample transportation date

characteristic: sample transportation time

characteristic: sampling time point

text: wastewater metagenome

text: wastewater treatment plant (municipal)

characteristic: sewage type

text: not provided

characteristic: size of the catchment area

characteristic: surveillance target

characteristic: surveillance target (scientific name)

text: 2022-W47 Jönköping Amplified SARS-CoV-2 RNA from wastewater

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: not provided

characteristic: area of sampling site

characteristic: collection date

text: Untreated wastewater was collected in Kalmar at Kalmarsundsverket on 2022-11-21 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Kalmar

characteristic: geographic location (region and locality)

text: virus

text: Kalmarsundsverket

text: wastewater metagenome

characteristic: organism

text: not provided

text: wastewater metagenome

text: wastewater treatment plant (municipal)

text: not provided

text: 2022-W47 Kalmar Amplified SARS-CoV-2 RNA from wastewater