characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Wellcome Sanger Institute

text: public

text: 60 to 65

text: ArrayExpress

characteristic: broker name

text: human

characteristic: description

text: adult

text: normal

text: wild type genotype

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: right cardiac atrium

characteristic: organism part

text: Homo sapiens

text: Male


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Wellcome Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Wellcome Sanger Institute

characteristic: Submitter Id

text: 60 to 65

characteristic: age

text: ArrayExpress

text: human

characteristic: common name

text: Protocols: Hearts were acquired after circulatory death (DCD) or after brain death (DBD). For DCD donors after confirmation of death there was a mandatory five-minute stand-off prior to sternotomy. In all cases the aorta was cross-clamped and cold cardioplegia solution was administered to the aortic root prior to cardiotomy. AV1, AV3, AV10, AV13 and AV14 were procured in the standard fashion and then immediately preserved with and transported on a hypothermic perfusion machine. AH2 was similarly preserved, but with immediate normothermic perfusion. It then underwent 4 hours of normothermic perfusion before samples were taken. For single nuclei sequencing, all donor samples were full-thickness myocardial tissues from the SAN, AVN, left and right atrium, left and right ventricles, interventricular septum and apex. Single nuclei were obtained from flash-frozen tissues using sectioning and mechanical homogenization. 5-10 mm thickness frozen tissues were first sectioned with cryostat in a 50 μm thickness section. All sections from each sample were homogenised using a 7 ml glass Dounce tissue grinder set (Merck) with 8–10 strokes of a loose pestle (A) and 8–10 strokes of a tight pestle (B) in homogenization buffer (250 mM sucrose, 25 mM KCl, 5 mM MgCl2, 10 mM Tris-HCl, 1 mM dithiothreitol (DTT), 1× protease inhibitor, 0.4 U μl−1 RNaseIn, 0.2 U μl−1 SUPERaseIn, 0.1% Triton X-100 in nuclease-free water). Homogenate was filtered through a 40-μm cell strainer (Corning). After centrifugation (500g, 5 min, 4 °C) the supernatant was removed and the pellet was resuspended in storage buffer (1× PBS, 4% bovine serum albumin (BSA), 0.2U μl−1 Protector RNaseIn). Nuclei were stained with 7-AAD Viability Staining Solution (BioLegend) and positive single nuclei were purified by fluorescent activated cell sorting (FACS) using MA900 Multi-Application Cell Sorter (Sony). Nuclei were further processed for 10x multiome paired RNA and ATACseq using the Chromium Controller (10X Genomics) according to the manufacturer’s protocol. cDNA library preparation was were carried out according to the manufacturer’s protocol.

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: heart left ventricle

text: Homo sapiens

text: Male

characteristic: sex

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Wellcome Sanger Institute

text: public

text: 45 to 50

text: ArrayExpress

characteristic: broker name

text: human

characteristic: description

text: adult

text: normal

text: wild type genotype

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: heart left ventricle

characteristic: organism part

text: Homo sapiens

text: Male


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Wellcome Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: 45 to 50

text: ArrayExpress

text: human

text: adult

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: apical region of left ventricle

text: Homo sapiens

text: Male


text: Wellcome Sanger Institute

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: 45 to 50

characteristic: age

text: ArrayExpress

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: left cardiac atrium

text: Homo sapiens

text: Male


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Wellcome Sanger Institute

text: public

text: 45 to 50

text: ArrayExpress

text: human

text: adult

text: normal

text: wild type genotype

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: heart right ventricle

text: Homo sapiens

text: Male

characteristic: sex

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Wellcome Sanger Institute

text: public

text: 45 to 50

text: ArrayExpress

characteristic: broker name

text: human

characteristic: description

text: adult

text: normal

text: wild type genotype

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: interventricular septum

characteristic: organism part

text: Homo sapiens

text: Male


text: Wellcome Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 45 to 50

text: ArrayExpress

text: human

text: adult

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: right cardiac atrium

text: Homo sapiens

text: Male


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Wellcome Sanger Institute

text: public

text: 45 to 50

characteristic: age

text: ArrayExpress

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: heart left ventricle

text: Homo sapiens

text: Female


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Wellcome Sanger Institute

text: public

text: 45 to 50

text: ArrayExpress

text: human

text: adult

text: normal

text: wild type genotype

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: heart right ventricle

text: Homo sapiens

text: Female

characteristic: sex

characteristic: title


text: Wellcome Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: 45 to 50

text: ArrayExpress

characteristic: broker name

text: human

characteristic: description

text: adult

text: normal

text: wild type genotype

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: right cardiac atrium

characteristic: organism part

text: Homo sapiens

text: Female


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Wellcome Sanger Institute

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 45 to 50

text: ArrayExpress

text: human

text: adult

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: sinoatrial node

text: Homo sapiens

text: Female


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Wellcome Sanger Institute

text: public

text: 45 to 50

characteristic: age

text: ArrayExpress

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: atrioventriculeft cardiac atriumr node

text: Homo sapiens

text: Female


text: Wellcome Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: 45 to 50

text: ArrayExpress

text: human

text: adult

text: normal

text: wild type genotype

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: interventricular septum

text: Homo sapiens

text: Female

characteristic: sex

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Wellcome Sanger Institute

text: public

text: Mixture of multiple donors

text: ArrayExpress

characteristic: broker name

text: human

characteristic: description

text: adult

text: normal

text: wild type genotype

characteristic: genotype

text: Mixture of AH1 and A61

text: Homo sapiens

text: Homo sapiens

text: sinoatrial node

characteristic: organism part

text: Homo sapiens

text: Female


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Wellcome Sanger Institute

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 70 to 75

text: ArrayExpress

text: human

text: adult

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: atrioventriculeft cardiac atriumr node

text: Homo sapiens

text: Female


text: Wellcome Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: 70 to 75

characteristic: age

text: ArrayExpress

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: sinoatrial node

text: Homo sapiens

text: Female


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Wellcome Sanger Institute

text: public

text: 20 to 25

text: ArrayExpress

text: human

text: adult

text: normal

text: wild type genotype

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: sinoatrial node

text: Homo sapiens

text: Male

characteristic: sex

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Wellcome Sanger Institute

text: public

text: 45 to 50

text: ArrayExpress

characteristic: broker name

text: human

characteristic: description

text: adult

text: normal

text: wild type genotype

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: sinoatrial node

characteristic: organism part

text: Homo sapiens

text: Male


text: Wellcome Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 60 to 65

text: ArrayExpress

text: human

text: adult

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: sinoatrial node

text: Homo sapiens

text: Female


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Wellcome Sanger Institute

text: public

text: 40 to 45

characteristic: age

text: ArrayExpress

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: sinoatrial node

text: Homo sapiens

text: Male


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Wellcome Sanger Institute

text: public

text: 40 to 45

text: ArrayExpress

text: human

text: adult

text: normal

text: wild type genotype

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: atrioventriculeft cardiac atriumr node

text: Homo sapiens

text: Male

characteristic: sex

characteristic: title


text: IP

characteristic: INSDC center name

characteristic: INSDC first public

text: public

unit: week

text: ArrayExpress

characteristic: broker name

text: house mouse

text: Protocols: Mice were sacrificed by cervical dislocation at the end of the study. The liver was carefully dissected and immediately frozen in liquid nitrogen for posterior RNA isolation Total RNA was isolated from 50-100 mg of snap-frozen liver tissue by the NucleoSpin RNA kit (Macherey-Nagel, 740955) Quality control was performed on an Agilent BioAnalyzer. Libraries were built using a TruSeq Stranded mRNA library Preparation Kit (Illumina, USA) following the manufacturer’s protocol

characteristic: description

text: MTFP1 -/-

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: liver

characteristic: organism part

text: Mus musculus

text: male

characteristic: strain


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IP

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: house mouse

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: liver

text: Mus musculus

text: male


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Wellcome Sanger Institute

text: public

text: 55 to 60

text: ArrayExpress

text: human

characteristic: common name

text: Protocols: Hearts were acquired after circulatory death (DCD) or after brain death (DBD). For DCD donors after confirmation of death there was a mandatory five-minute stand-off prior to sternotomy. In all cases the aorta was cross-clamped and cold cardioplegia solution was administered to the aortic root prior to cardiotomy. AV1, AV3, AV10, AV13 and AV14 were procured in the standard fashion and then immediately preserved with and transported on a hypothermic perfusion machine. AH2 was similarly preserved, but with immediate normothermic perfusion. It then underwent 4 hours of normothermic perfusion before samples were taken. For single nuclei sequencing, all donor samples were full-thickness myocardial tissues from the SAN, AVN, left and right atrium, left and right ventricles, interventricular septum and apex. Single nuclei were obtained from flash-frozen tissues using sectioning and mechanical homogenization. 5-10 mm thickness frozen tissues were first sectioned with cryostat in a 50 μm thickness section. All sections from each sample were homogenised using a 7 ml glass Dounce tissue grinder set (Merck) with 8–10 strokes of a loose pestle (A) and 8–10 strokes of a tight pestle (B) in homogenization buffer (250 mM sucrose, 25 mM KCl, 5 mM MgCl2, 10 mM Tris-HCl, 1 mM dithiothreitol (DTT), 1× protease inhibitor, 0.4 U μl−1 RNaseIn, 0.2 U μl−1 SUPERaseIn, 0.1% Triton X-100 in nuclease-free water). Homogenate was filtered through a 40-μm cell strainer (Corning). After centrifugation (500g, 5 min, 4 °C) the supernatant was removed and the pellet was resuspended in storage buffer (1× PBS, 4% bovine serum albumin (BSA), 0.2U μl−1 Protector RNaseIn). Nuclei were stained with 7-AAD Viability Staining Solution (BioLegend) and positive single nuclei were purified by fluorescent activated cell sorting (FACS) using MA900 Multi-Application Cell Sorter (Sony). Nuclei were further processed for 10x multiome paired RNA and ATACseq using the Chromium Controller (10X Genomics) according to the manufacturer’s protocol. ATAC library preparation was were carried out according to the manufacturer’s protocol.

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: heart left ventricle

text: Homo sapiens

text: male


text: Wellcome Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: 60 to 65

text: ArrayExpress

characteristic: broker name

text: human

characteristic: description

text: adult

text: normal

text: wild type genotype

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: right cardiac atrium

text: Homo sapiens

text: male

characteristic: sex

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Wellcome Sanger Institute

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 60 to 65

text: ArrayExpress

text: human

text: adult

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: heart left ventricle

characteristic: organism part

text: Homo sapiens

text: male


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Wellcome Sanger Institute

text: public

characteristic: Submitter Id

text: 45 to 50

characteristic: age

text: ArrayExpress

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: heart left ventricle

text: Homo sapiens

text: male


text: Wellcome Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: 45 to 50

text: ArrayExpress

characteristic: broker name

text: human

text: adult

text: normal

characteristic: disease

text: wild type genotype

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: apical region of left ventricle

text: Homo sapiens

text: male


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Wellcome Sanger Institute

text: public

text: 45 to 50

text: ArrayExpress

text: human

characteristic: description

text: adult

text: normal

text: wild type genotype

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: left cardiac atrium

text: Homo sapiens

text: male

characteristic: sex

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Wellcome Sanger Institute

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 45 to 50

text: ArrayExpress

text: human

text: adult

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: heart right ventricle

characteristic: organism part

text: Homo sapiens

text: male


text: Wellcome Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: 45 to 50

characteristic: age

text: ArrayExpress

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: interventricular septum

text: Homo sapiens

text: male


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Wellcome Sanger Institute

text: public

text: 45 to 50

text: ArrayExpress

characteristic: broker name

text: human

text: adult

text: normal

characteristic: disease

text: wild type genotype

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: right cardiac atrium

text: Homo sapiens

text: male


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Wellcome Sanger Institute

text: public

text: 45 to 50

text: ArrayExpress

text: human

characteristic: description

text: adult

text: normal

text: wild type genotype

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: heart left ventricle

text: Homo sapiens

text: female

characteristic: sex

characteristic: title


text: Wellcome Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 45 to 50

text: ArrayExpress

text: human

text: adult

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: heart right ventricle

characteristic: organism part

text: Homo sapiens

text: female


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Wellcome Sanger Institute

text: public

characteristic: Submitter Id

text: 45 to 50

characteristic: age

text: ArrayExpress

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: right cardiac atrium

text: Homo sapiens

text: female


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Wellcome Sanger Institute

text: public

text: 45 to 50

text: ArrayExpress

characteristic: broker name

text: human

text: adult

text: normal

characteristic: disease

text: wild type genotype

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: sinoatrial node

text: Homo sapiens

text: female


text: Wellcome Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: 45 to 50

text: ArrayExpress

text: human

characteristic: description

text: adult

text: normal

text: wild type genotype

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: atrioventricular node

text: Homo sapiens

text: female

characteristic: sex

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Wellcome Sanger Institute

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 45 to 50

text: ArrayExpress

text: human

text: adult

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: interventricular septum

characteristic: organism part

text: Homo sapiens

text: female


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Wellcome Sanger Institute

text: public

characteristic: Submitter Id

text: Mixture of multiple donors

characteristic: age

text: ArrayExpress

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: normal

text: wild type genotype

text: Mixture of AH1 and A61

text: Homo sapiens

text: Homo sapiens

text: sinoatrial node

text: Homo sapiens

text: female


text: Wellcome Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: 70 to 75

text: ArrayExpress

characteristic: broker name

text: human

text: adult

text: normal

characteristic: disease

text: wild type genotype

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: atrioventricular node

text: Homo sapiens

text: female


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Wellcome Sanger Institute

text: public

text: 70 to 75

text: ArrayExpress

text: human

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text: adult

text: normal

text: wild type genotype

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: sinoatrial node

text: Homo sapiens

text: female

characteristic: sex

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Wellcome Sanger Institute

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 20 to 25

text: ArrayExpress

text: human

text: adult

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: sinoatrial node

characteristic: organism part

text: Homo sapiens

text: male


text: Wellcome Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: 45 to 50

characteristic: age

text: ArrayExpress

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: sinoatrial node

text: Homo sapiens

text: male


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Wellcome Sanger Institute

text: public

text: 60 to 65

text: ArrayExpress

characteristic: broker name

text: human

text: adult

text: normal

characteristic: disease

text: wild type genotype

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: sinoatrial node

text: Homo sapiens

text: female


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Wellcome Sanger Institute

text: public

text: 40 to 45

text: ArrayExpress

text: human

characteristic: description

text: adult

text: normal

text: wild type genotype

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: sinoatrial node

text: Homo sapiens

text: male

characteristic: sex

characteristic: title


text: Wellcome Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 40 to 45

text: ArrayExpress

text: human

text: adult

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: atrioventricular node

characteristic: organism part

text: Homo sapiens

text: male


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Instituto de la Grasa - CSIC

text: public

tag: Namespace:Instituto de la Grasa - CSIC

characteristic: Submitter Id

text: Food research institution

characteristic: broad-scale environmental context

characteristic: collection date

text: Table olive fermenting brine

text: Table olive fermenting brine

characteristic: environmental medium

text: Spain

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: 5-L fermenter at pilot plant

characteristic: local environmental context

text: food fermentation metagenome

text: Chemical and microbial characterization of spontaneous fermentations of Natural-style green olives from Gordal, Hojiblanca and Manzanilla cultivars

characteristic: project name

text: food fermentation metagenome


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Instituto de la Grasa - CSIC

characteristic: INSDC center name

text: public

text: Food research institution

text: Table olive fermenting brine

characteristic: description

text: Table olive fermenting brine

text: Spain

text: 5-L fermenter at pilot plant

text: food fermentation metagenome

characteristic: organism

text: Chemical and microbial characterization of spontaneous fermentation of Natural-style green olives from Gordal, Hojiblanca and Manzanilla cultivars

text: food fermentation metagenome

characteristic: title


characteristic: ENA-LAST-UPDATE

text: Roulin Group

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Roulin Group

text: stiff brome

characteristic: common name

text: France

text: Brachypodium distachyon

text: Brachypodium distachyon


characteristic: External Id

text: Roulin Group

text: public

characteristic: Submitter Id

text: stiff brome

text: Greece

characteristic: geographic location (country and/or sea)

text: Brachypodium distachyon

text: Brachypodium distachyon


text: Roulin Group

text: public

text: stiff brome

text: Montenegro

text: Brachypodium distachyon

text: Brachypodium distachyon


text: public

text: E-MTAB-12938:NG-6612_R1_dnCad_lib21093_1481_2

tag: Namespace:Institut de Biologie Valrose, iBV UMR CNRS 7277/Inserm 1091/Universite Cote d’Azur

unit: hour

characteristic: age

text: ArrayExpress

characteristic: broker name

text: common urchin

text: Protocols: Two independent batches of embryos were used for each condition. Embryos with DN-cadherin mRNA were collected at the early blastula stage (6h30 after fertilization at 18°C). RNA was purified using Trizol. Libraries were made by the GATC company. Briefly, polyadenylated transcripts were purified by two rounds of oligo-dT chromatography and cDNA libraries were constructed using a Superscript double stranded cDNA synthesis kit (Invitrogen), fragmented by sonication, and cDNA fragments of 200-500 bp were gel purified. Illumina adaptaters were ligated to these fragments.

text: blastula

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Paracentrotus lividus

text: Paracentrotus lividus

text: whole organism

characteristic: organism part

text: overexpression of dominant negative cadherin

characteristic: phenotype

text: A

characteristic: replicate

text: Paracentrotus lividus

text: dnCad

characteristic: stimulus


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Institut de Biologie Valrose, iBV UMR CNRS 7277/Inserm 1091/Universite Cote d’Azur

characteristic: INSDC center name

text: public

text: E-MTAB-12938:NG-6612_R1_NI_lib21094_1481_2

text: ArrayExpress

text: common urchin

text: Protocols: Two independent batches of embryos were used for each condition. Embryos from control cultures (uninjected) mRNA were collected at the early blastula stage (6h30 after fertilization at 18°C). RNA was purified using Trizol. Libraries were made by the GATC company. Briefly, polyadenylated transcripts were purified by two rounds of oligo-dT chromatography and cDNA libraries were constructed using a Superscript double stranded cDNA synthesis kit (Invitrogen), fragmented by sonication, and cDNA fragments of 200-500 bp were gel purified. Illumina adaptaters were ligated to these fragments.

characteristic: description

text: blastula

text: wild type genotype

text: Paracentrotus lividus

characteristic: organism

text: Paracentrotus lividus

text: whole organism

text: wild type

text: B

text: Paracentrotus lividus

text: non injected

characteristic: title


characteristic: External Id

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12938:NG-6612_R1_Pmar_lib21092_1481_3

characteristic: Submitter Id

text: ArrayExpress

text: common urchin

characteristic: common name

text: Protocols: Two independent batches of embryos were used for each condition. Embryos injected with pmar1 mRNA were collected at the early blastula stage (6h30 after fertilization at 18°C). RNA was purified using Trizol. Libraries were made by the GATC company. Briefly, polyadenylated transcripts were purified by two rounds of oligo-dT chromatography and cDNA libraries were constructed using a Superscript double stranded cDNA synthesis kit (Invitrogen), fragmented by sonication, and cDNA fragments of 200-500 bp were gel purified. Illumina adaptaters were ligated to these fragments.

text: blastula

text: wild type genotype

text: Paracentrotus lividus

text: Paracentrotus lividus

text: whole organism

text: overexpression of Pmar1

text: C

text: Paracentrotus lividus

text: Pmar


text: public

text: E-MTAB-12938:NG-6612_R2_dnCad_lib21096_1481_3

characteristic: age

text: ArrayExpress

characteristic: broker name

text: common urchin

text: blastula

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Paracentrotus lividus

text: Paracentrotus lividus

text: whole organism

characteristic: organism part

characteristic: phenotype

text: A

text: Paracentrotus lividus

text: dnCad


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC center name

text: public

text: E-MTAB-12938:NG-6612_R2_NI_lib21097_1481_2

text: ArrayExpress

text: common urchin

text: blastula

text: wild type genotype

text: Paracentrotus lividus

text: Paracentrotus lividus

text: whole organism

text: wild type

text: B

characteristic: replicate

text: Paracentrotus lividus

text: non injected

characteristic: stimulus


characteristic: External Id

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-12938:NG-6612_R2_Pmar_lib21095_1481_3

text: ArrayExpress

text: common urchin

characteristic: description

text: blastula

text: wild type genotype

text: Paracentrotus lividus

characteristic: organism

text: Paracentrotus lividus

text: whole organism

text: C

text: Paracentrotus lividus

text: dnCad

characteristic: title


text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12938:NG-6612_R2_Pmar_lib22925_1566_6

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: common urchin

characteristic: common name

text: blastula

text: wild type genotype

text: Paracentrotus lividus

text: Paracentrotus lividus

text: whole organism

text: C

text: Paracentrotus lividus

text: dnCad


status: KILLED

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: killed

tag: Namespace:Klinikum rechts der Isar, II. Medizinische Klinik

text: human

text: Macrodissected gastric FFPE tumor tissue of patient 1N7T85/ S-2007-03592-I-A44

text: Homo sapiens

text: Homo sapiens

text: RNASample


characteristic: ENA-LAST-UPDATE

text: Klinikum rechts der Isar, II. Medizinische Klinik

characteristic: INSDC center name

text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient 2G9VAK/ S-H-2010-06940-VI-A

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient 2J8UCN/ S-H-2012-18333-II-C

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient 4SENA7/ S-H-2005-13975, technical replicate 1

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient 4SENA7/ S-2005-13975-I-B, technical replicate 2

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient 4T2UZB/ S-H-2005-1146

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient 6P4EWP/ S-5697-07-I-H

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient 71E92J/ S-H-2007-00445-I-F

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient 8YXUYT/ S-2018-00340

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient AFC5B1/ S-2010-25993-II-A4-3

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient BCNHE6_ADC/ S-2014-11416-I-D-Exo

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient BCNHE6_NEC/ S-2014-11416-I-D-Endo

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient BU3KHJ/ S-2014-09150-III-A

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient C126AL/ S-2012-35012-II-A4-2

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient E6F6UP/ S-2007-07153-A5-5

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient F9TDLJ/ S-6814-08-II-D

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient FMFW76/ S-2010-43364-IV-C1, technical replicate 1

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: Klinikum rechts der Isar, II. Medizinische Klinik

text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient FMFW76/ S-2010-43364, technical replicate 2

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient G82WKG/ S-2001-5713

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient GMNY48/ S-2018-13503-I-K

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient GX4VZC/ S-H-2016-18461-III

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient J837KK/ S-K250-A

text: Homo sapiens

text: Homo sapiens

text: RNASample


JCTJTD

text: killed

text: JCTJTD

text: human

text: Macrodissected gastric FFPE tumor tissue of patient JCTJTD/ S-H-2019-5981

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient KHEY75/ S-K-2017-00049

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient KQAA8F/ S-K250-D

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient MGJ67Y/ S-H-2013-05505-III-E

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient N6PH7S/ S-K-2015-00307-K

text: Homo sapiens

text: Homo sapiens

text: RNASample


NBZZKE

text: killed

text: NBZZKE

text: human

text: Macrodissected gastric FFPE tumor tissue of patient NBZZKE/ S-2017-05077

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient P4GNJ9/ S-15-39117-A-3

text: Homo sapiens

text: Homo sapiens

text: RNASample


PPPVLZ

text: killed

text: PPPVLZ

text: human

text: Macrodissected gastric FFPE tumor tissue of patient PPPVLZ/ S-2005-8171

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient Q2J3TB/ S-2017-00538-II-R

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient TT2LNJ/ S-H2018-8406

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient V9A3BS/ S-2005-15844-I-A2

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: Klinikum rechts der Isar, II. Medizinische Klinik

text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient VTSJV1/ S-2003-9189-A, technical replicate 1

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient VTSJV1/ S-2003-9189, technical replicate 2

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient XYT5J7/ S-H-2011-19012-II-B

text: Homo sapiens

text: Homo sapiens

text: RNASample


YHJGYY

text: killed

text: YHJGYY

text: human

text: Macrodissected gastric FFPE tumor tissue of patient YHJGYY/ S-H-2015-18618

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient G82WKG/ 2001-5713_S14_R1_001

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient VTSJV1/ 2003-9189_S18_R1_001

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient PPPVLZ/ 2005-8171_S13_R1_001

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient 4SENA7/ 2005-13975_S10_R1_001

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient FMFW76/ 2010-43364_S16_R1_001

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient BCNHE6_Endo/ 2014-11416_S1_R1_001

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient NBZZKE/ 2017-05077_S2_R1_001

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient GMNY48/ 2018-13503_S17_R1_001

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient 4T2UZB/ H-2005-1146_S11_R1_001

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient 71E92J/ H-2007-00445_S8_R1_001

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient 2G9VAK/ H-2010-06940_S9_R1_001

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient 2J8UCN/ H-2012-18333_S5_R1_001

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: Klinikum rechts der Isar, II. Medizinische Klinik

text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient JCTJTD/ H-2019-5981_S15_R1_001

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient TT2LNJ/ H2018-8406_S19_R1_001

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient J837KK/ K250_A_S3_R1_001

text: Homo sapiens

text: Homo sapiens

text: RNASample


text: killed

text: human

text: Macrodissected gastric FFPE tumor tissue of patient KQAA8F/ K250_D_S7_R1_001

text: Homo sapiens

text: Homo sapiens

text: RNASample


E-MTAB-12954:Acute (Figure 6)

text: public

text: E-MTAB-12954:Acute (Figure 6)

tag: Namespace:Peter Doherty Institute for Infection & Immunity, University of Melbourne

text: 6 to 12

text: ArrayExpress

text: erythrocyte

text: house mouse

text: Protocols: Peripheral blood (5-6 drops) was collected into 1ml cold RPMI medium containing 5 U/mL heparin by tail vein bleed. Diluted blood (50 μL) was further diluted in 2 mL cold 1% BSA/PBS and RBCs isolated away from leukocytes by cell sorting. RBCs cells were then sorted as single-cells using the Chromium 10X Genomics platform. Approximately 5,000 RBCs (red blood cells), some of which were parasitized, were loaded per channel onto a Chromium controller (10x Genomics) for generation of gel bead-in-emulsions Sequencing libraries were prepared using Single Cell 3’ Reagent Kits v3 or where specified v2 (10x Genomics).

text: wild type genotype

characteristic: genotype

text: prior acute Plasmodium berghei ANKA infection

characteristic: growth condition

text: Plasmodium berghei ANKA

characteristic: infect

text: Mus musculus

text: Mus musculus

text: blood

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

characteristic: strain

text: Acute (Figure 6)


E-MTAB-12954:Acute (Supp Figure 5)

characteristic: External Id

text: Peter Doherty Institute for Infection & Immunity, University of Melbourne

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-12954:Acute (Supp Figure 5)

text: 6 to 12

text: ArrayExpress

text: erythrocyte

text: house mouse

characteristic: description

text: wild type genotype

text: Plasmodium berghei ANKA

text: Mus musculus

characteristic: organism

text: Mus musculus

text: blood

text: Mus musculus

text: female

text: Acute (Supp Figure 5)

characteristic: title


E-MTAB-12954:LPS (Figure 6)

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12954:LPS (Figure 6)

characteristic: Submitter Id

text: 6 to 12

characteristic: age

text: ArrayExpress

characteristic: broker name

text: erythrocyte

text: house mouse

characteristic: common name

text: wild type genotype

text: prior lipopolysaccharide conditioning

text: Plasmodium berghei ANKA

text: Mus musculus

text: Mus musculus

text: blood

text: Mus musculus

text: female

text: lipopolysaccharide

characteristic: stimulus

text: LPS (Figure 6)


E-MTAB-12954:Naive (Figure 6)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC center name

text: public

text: E-MTAB-12954:Naive (Figure 6)

text: 6 to 12

text: ArrayExpress

text: erythrocyte

text: house mouse

text: wild type genotype

characteristic: genotype

text: Plasmodium berghei ANKA

characteristic: infect

text: Mus musculus

text: Mus musculus

text: blood

text: Mus musculus

text: female

text: Naive (Figure 6)


E-MTAB-12954:Naive (Supp Figure 5)

characteristic: External Id

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-12954:Naive (Supp Figure 5)

text: 6 to 12

text: ArrayExpress

text: erythrocyte

text: house mouse

text: wild type genotype

text: Plasmodium berghei ANKA

text: Mus musculus

text: Mus musculus

text: blood

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

characteristic: strain

text: Naive (Supp Figure 5)


E-MTAB-12954:Rag1-/- (Figure 5)

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12954:Rag1-/- (Figure 5)

characteristic: Submitter Id

text: 6 to 12

text: ArrayExpress

text: erythrocyte

text: house mouse

characteristic: description

text: Rag1 -/-

text: Plasmodium berghei ANKA

text: Mus musculus

characteristic: organism

text: Mus musculus

text: blood

text: Mus musculus

text: female

text: Rag1-/- (Figure 5)

characteristic: title


E-MTAB-13009:Sample 1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-13009:Sample 1

tag: Namespace:Vinca Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

text: Protocols: After culture, cells were rinsed with PBS total RNA was isolated according to TRI Reagent protocol. Cell lines were maintained at 37˚C, 95% humidity and 5% CO2 in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. Total RNA was isolated according to TRI Reagent protocol. The SMARTer smRNA-Seq Kit for Illumina is used to generate small RNA-seq libraries for sequencing on iSeq100 Illumina platform. A total of 500ng of total RNA was used as input for library construction. Illumina adapter and index sequences are incorporated in a ligation-free manner during library amplification, ensuring that diverse smRNA species are represented with minimal bias.

text: embryo

characteristic: developmental stage

text: MIR185 knockout

characteristic: genetic modification

text: CRISPR-Cas9 induced MIR185 gene knockout

text: Homo sapiens

text: Homo sapiens

text: kidney

text: Homo sapiens

text: Sample 1


E-MTAB-13009:Sample 2

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Vinca Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-13009:Sample 2

text: ArrayExpress

text: human

text: embryo

text: MIR185 knockout

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: kidney

text: Homo sapiens

text: Sample 2


E-MTAB-13009:Sample 3

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-13009:Sample 3

text: ArrayExpress

text: human

text: embryo

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: kidney

characteristic: organism part

text: Homo sapiens

text: Sample 3


E-MTAB-13009:Sample 4

text: public

characteristic: SRA accession

text: E-MTAB-13009:Sample 4

characteristic: Submitter Id

text: ArrayExpress

text: human

characteristic: description

text: embryo

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: kidney

text: Homo sapiens

text: Sample 4


E-MTAB-13009:Sample 5

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-13009:Sample 5

text: ArrayExpress

text: human

text: embryo

text: MIR484 knockdown

text: CRISPR-Cas9 induced MIR484 gene knockdown

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: kidney

text: Homo sapiens

text: Sample 5

characteristic: title


E-MTAB-13009:Sample 6

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-13009:Sample 6

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

text: embryo

text: MIR484 knockdown

text: Homo sapiens

text: Homo sapiens

text: kidney

text: Homo sapiens

text: Sample 6


characteristic: ENA-LAST-UPDATE

text: Researcher

text: public

text: ArrayExpress

text: mononuclear cell

text: cattle

text: Protocols: Peripheral blood mononuclear cells (PBMC) were prepared from heparinised blood using Histopaque and frozen in foetal calf serum supplemented with 10% DMSO. Cells were defrosted into warm RPMI GlutaMAX HEPES media supplemented with 10% foetal calf serum, 1 mM sodium pyruvate, 50 µM 2-mercaptoethanol, 100 IU/mL penicillin and 100 µg/mL streptomycin (RPMI/10). Nucleic acid was extracted from one million cells using a MagMAX mirvRNA Total RNA extraction kit on a MagMAX™ Express-96 Deep Well Magnetic Particle Processor. 3’UTR sequencing

text: challenge with viral infection

characteristic: growth condition

characteristic: individual

text: Bos taurus

text: Bos taurus

text: blood

text: Bos taurus


text: Streptococcus pneumoniae

characteristic: ArrayExpress-SPECIES

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Streptococcus pneumoniae

text: Streptococcus pneumoniae

text: Streptococcus pneumoniae


characteristic: INSDC last update

text: public

characteristic: INSDC status

tag: Namespace:Institute for Chemistry and Biology of the Marine Environment

text: Coastal Sea phytoplankton spring bloom

characteristic: broad-scale environmental context

characteristic: collection date

text: North Sea spring bloom; size-fractionated 0.2-3µm

text: North Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mesocosm

characteristic: local environmental context

text: artificial sea water

characteristic: medium

text: marine metagenome

text: EcoMol Planktoron 16S amplicons

characteristic: project name

text: marine metagenome


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Institute for Chemistry and Biology of the Marine Environment

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Coastal Sea phytoplankton spring bloom

text: North Sea spring bloom; size-fractionated 3-8µm

characteristic: description

text: North Sea

text: mesocosm

text: artificial sea water

text: marine metagenome

characteristic: organism

text: EcoMol Planktoron 16S amplicons

text: marine metagenome

characteristic: title


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Coastal Sea phytoplankton spring bloom

characteristic: broad-scale environmental context

text: North Sea

text: mesocosm

text: artificial sea water

text: marine metagenome

text: EcoMol Planktoron 16S amplicons

text: marine metagenome


E-MTAB-12959:HT-418 Explant Day 0

text: public

text: E-MTAB-12959:HT-418 Explant Day 0

tag: Namespace:Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI

unit: day

characteristic: age

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

text: Protocols: For scRNA-sequencing of Lung ALI explant cultures, the outside rind of n = 3 explants at each timepoint (0, 3, 6, 9 and 12 days) was micro dissected and minced using a No.1 Scalpel, discarding the center of the explant, which appeared necrotic in later timepoints. Pooled explants at each timepoint (0, 3, 6, 9 and 12 days) were dissociated using the Neural Tissue Dissociation Kit (P) (Mitenyi Biotec, Cat#130092628). Briefly minced explant tissue was resuspended in Mix 1 and incubated at 37ºC for 15 minutes. Mix 2 was then added and incubated for 10 minutes at 37ºC. Cells were agitated by P1000 pipetting and then returned to the incubator for additional 10 minute incubations at 37ºC, followed by P1000 pipetting until a single cell suspension was achieved (~30 minutes). Obtained single cell suspension was filtered through a 70 µm Flowmi Cell Stainer (Sigma, Cat#BAH136800070) and then resuspended in Red Blood Cell Lysis Buffer (Roche, 11814389001) for 15 minutes at 4ºC. After Red Blood Cell Lysis, cells were washed twice with 2mL 1X HBSS + 1% BSA and then resuspended in Cryostor-CS10 (Sigma, Cat#C2874) for storage in liquid nitrogen. All Lung ALI explant culture samples were thawed and co-submitted for sequencing on the same day. Thawing of cells prior to sequencing consisted of adding 1:1 increments of RPMI + 10% FBS drop-wise, with 1 minute pauses every time the volume doubled, until a total volume of 32 mLs was achieved. Cells were then pelleted and resuspended in 1 mL HBSS + 1% BSA and passed through a 40 µm Flowmi Cell Strainer (Sigma, Cat#BAH136800040), counted on a hemocytometer and submitted at 1000 cells/µl in HBSS + 1% BSA to the University of Michigan Advanced Genomics Core for library preparation by the Chromium Next GEM Single Cell 3’ GEM, Library and Gel Bead Kit v3.1 (10x Genomics, Cat#PN1000128) targeting 7500 cells. For ALI explant cultures (2725-2, 2725-3, 2725-4, 2725-5) small pieces (< 0.5 cm diameter) of canalicular stage human lung (122 days post conception) were dissected from distal regions and placed on Nucleopore Track-Etched Membrane disks (13 mm, 8 µm pore, poly-carbonate) (Sigma, Cat#WHA110414) floating on top of 500 µl of human lung ALI explant media (Advanced DMEM/F-12 (Thermo Fisher, Cat#12634010), 2 mM Glutamax (Thermo Fisher, Cat#35050061), 15 mM HEPES (Corning, Cat#25060CI), 1X B27 Supplement (Thermo Fisher, Cat#17504044) 1X N-2 Supplement (Thermo Fisher, Cat#17502048), 100U/mL penicillin-streptomycin (Thermo Fisher, Cat#15140122)) in a 24-well tissue culture plate (Thermo Fisher, Cat#12565163). Immediately after tissue dissociation, single cell libraries were prepared on the 10x Chromium with a target of 7500 cells. Chromium Single Cell 3’ Library & Gel Bead Kit v3.1 (10x Genomics, Cat#PN1000128) following the manufacturer's protocol. Immediately after tissue dissociation, single cell libraries were prepared on the 10x Chromium with a target of 7500 cells. Chromium Single Cell 3’ Library & Gel Bead Kit v3.1 (10x Genomics, Cat#PN1000128) following the manufacturer's protocol.

text: Fetal Stage

characteristic: developmental stage

text: normal

characteristic: disease

text: lung explant air-liquid interface culture

characteristic: growth condition

text: Homo sapiens

text: Homo sapiens

text: lung

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex

text: HT-418 Explant Day 0


E-MTAB-12959:HT-418 Explant Day 12

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI

text: public

characteristic: SRA accession

text: E-MTAB-12959:HT-418 Explant Day 12

characteristic: Submitter Id

text: ArrayExpress

text: human

characteristic: description

text: Fetal Stage

text: normal

text: Homo sapiens

text: Homo sapiens

text: lung

text: Homo sapiens

text: male

text: HT-418 Explant Day 12


E-MTAB-12959:HT-418 Explant Day 3

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-12959:HT-418 Explant Day 3

text: ArrayExpress

text: human

text: Fetal Stage

text: normal

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: lung

text: Homo sapiens

text: male

text: HT-418 Explant Day 3

characteristic: title


E-MTAB-12959:HT-418 Explant Day 6

text: public

characteristic: INSDC status

text: E-MTAB-12959:HT-418 Explant Day 6

characteristic: age

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

text: Fetal Stage

characteristic: developmental stage

text: normal

text: Homo sapiens

text: Homo sapiens

text: lung

text: Homo sapiens

text: male

text: HT-418 Explant Day 6


E-MTAB-12959:HT-418 Explant Day 9

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-12959:HT-418 Explant Day 9

text: ArrayExpress

text: human

text: Fetal Stage

text: normal

characteristic: disease

characteristic: growth condition

text: Homo sapiens

text: Homo sapiens

text: lung

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex

text: HT-418 Explant Day 9


E-MTAB-12960:CK + AB Day 1

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: SRA accession

text: E-MTAB-12960:CK + AB Day 1

characteristic: Submitter Id

text: ArrayExpress

text: type II pneumocyte

text: human

text: Protocols: Organoids were dislodged from Matrigel by mechanical dissociation using a p1000 pipette tip. Pelleted organoids were resuspended in TrypLE Express (Thermo Fisher, Cat #17105041) and incubated at 37ºC, pipetting gently at 5 minute intervals until a single cell suspension is obtained (~10 minutes). Cells were washed 3x with Hanks Balanced Salt Solution (HBSS) (Thermo Fisher, Cat #14175095) + 1% BSA and then passed through a 40 µm FlowMi Cell Strainer. Cells were counted on a hemocytometer and then resuspended at 1000 cells/µl in HBSS + 1% BSA for submission to the the University of Michigan Sequencing Core, which prepared libraries using the Chromium Next GEM Single Cell 3’ GEM, Library and Gel Bead Kit v3.1 (10x Genomics, Cat#PN1000128) targeting 3500 cells. All organoids in this study were cultured in 8 mg/mL Matrigel growth matrix (Corning Cat#354234), fed every three days with indicated media and passaged into fresh Matrigel every 7 - 10 days by needle sheering. Bud tip Progenitor Media (3F): DMEM/F-12 (Corning, Cat#10-092-CV), 100U/mL penicillin-streptomycin (Thermo Fisher, Cat#15140122), 1X B-27 supplement (Thermo Fisher, Cat#17504044), 1X N2 supplement (Thermo Fisher, Cat#17502048), 0.05% BSA (Sigma, Cat#A9647), 50µg/mL L-ascorbic acid (Sigma,Cat#A4544), 0.4 µM 1-Thioglycerol (Sigma, Cat#M1753), 50nM all-trans retinoic acid (Sigma, Cat#R2625), 10ng/mL recombinant human FGF7 (R&D Systems, Cat#251-KG), and 3µM CHIR99021 (APExBIO, Cat#A3011). CK + AB Media: DMEM/F-12 (Corning, Cat#10-092-CV), 100U/mL penicillin-streptomycin (Thermo Fisher, Cat#15140122),1X B27 without vitamin A (Thermo Fisher, Cat# 12587010) , 1X N2 supplement (Thermo Fisher, Cat#17502048), 0.05% BSA (Sigma, Cat#A9647), 50µg/mL L-ascorbic acid (Sigma,Cat#A4544), 0.4 µM 1-Thioglycerol (Sigma, Cat#M1753), 10ng/mL recombinant human FGF7 (R&D Systems, Cat#251-KG), and 3µM CHIR99021 (APExBIO, Cat#A3011), 1 µM A-8301 (APExBIO Cat#A3133), 100 ng/mL BMP4 (R&D Systems Cat#314-BP-050). CK + DCI Media: DMEM/F-12 (Corning, Cat#10-092-CV), 100U/mL penicillin-streptomycin (Thermo Fisher, Cat#15140122),1X B27 without vitamin A (Thermo Fisher, Cat# 12587010) , 1X N2 supplement (Thermo Fisher, Cat#17502048), 0.05% BSA (Sigma, Cat#A9647), 50µg/mL L-ascorbic acid (Sigma,Cat#A4544), 0.4 µM 1-Thioglycerol (Sigma, Cat#M1753), 10ng/mL recombinant human FGF7 (R&D Systems, Cat#251-KG), and 3µM CHIR99021 (APExBIO, Cat#A3011),50 nM Dexamethasone (Sigma, Cat#D4902), 100 nM 3-isobutyl-1-methylxanthine (Sigma, Cat#I5879) and 100 nM 8-Bromoadensoine 3’,5’-cyclic monophosphate sodium salt (Sigma, Cat#B7880). SFFF without FGF10 Media: Advanced DMEM/F12, 2 mM Glutamax, 1X B27 supplement, 100 U/mL penicillin-streptomycin, 15 mM HEPES, 0.05% BSA, 10 µM TGF-inhibitor SB43152 (APExBIO, Cat#A8249), 1 µM p38 MAP kinase-inhibitor BIRB796 (APExBIO, Cat#A5639), 3 µM CHIR99021, 50 ng/mL rhEGF (R&D Systems, Cat#236-EG). Lung progenitor (bud tip) organoids were derived and maintained in vitro for at least 20 days prior to differentiation. For CK + AB and CK + DCI treatments, organoids were passaged to fresh matrigel, given three days to recover and then switched from Bud tip progenitor media to CK + AB or CK + DCI for 21 days, with passages occurring at days 7 and 14. At day 21, CK + AB or CK + DCI treated organoids were passaged to new matrigel and switched to SFFF without FGF10 for an additional 120 days. Immediately after organoid dissociation, single cell libraries were prepared on the 10x Chromium with a target of 3500 cells. Chromium Single Cell 3’ Library & Gel Bead Kit v3.1 (10x Genomics, Cat#PN1000128) following the manufacturer's protocol. Immediately after organoid dissociation, single cell libraries were prepared on the 10x Chromium with a target of 3500 cells. Chromium Single Cell 3’ Library & Gel Bead Kit v3.1 (10x Genomics, Cat#PN1000128) following the manufacturer's protocol.

characteristic: description

text: fetal stage

text: normal

text: CK + AB media organoid culture

text: Homo sapiens

text: Homo sapiens

text: lung

text: lung epithelial progenitor

characteristic: progenitor cell type

text: Homo sapiens

text: male

text: CK + AB Day 1


E-MTAB-12960:CK + AB Day 120

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-12960:CK + AB Day 120

text: ArrayExpress

text: type II pneumocyte

text: human

text: fetal stage

text: normal

text: SFFF without FGF10 media organoid culture

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: lung

text: lung epithelial progenitor

text: Homo sapiens

text: male

text: CK + AB Day 120

characteristic: title


E-MTAB-12960:CK + AB Day 21

characteristic: ENA-CHECKLIST

text: public

characteristic: INSDC status

text: E-MTAB-12960:CK + AB Day 21

characteristic: age

text: ArrayExpress

characteristic: broker name

text: type II pneumocyte

text: human

characteristic: common name

text: fetal stage

characteristic: developmental stage

text: normal

text: CK + AB media organoid culture

text: Homo sapiens

text: Homo sapiens

text: lung

text: lung epithelial progenitor

text: Homo sapiens

text: female

text: CK + AB Day 21


E-MTAB-12960:CK + AB Day 6

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-12960:CK + AB Day 6

text: ArrayExpress

text: type II pneumocyte

text: human

text: fetal stage

text: normal

characteristic: disease

text: CK + AB media organoid culture

characteristic: growth condition

text: Homo sapiens

text: Homo sapiens

text: lung

characteristic: organism part

text: lung epithelial progenitor

text: Homo sapiens

text: male

characteristic: sex

text: CK + AB Day 6


E-MTAB-12960:CK + DCI Day 1

characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: SRA accession

text: E-MTAB-12960:CK + DCI Day 1

characteristic: Submitter Id

text: ArrayExpress

text: type II pneumocyte

text: human

characteristic: description

text: fetal stage

text: normal

text: CK + DCI media organoid culture

text: Homo sapiens

text: Homo sapiens

text: lung

text: lung epithelial progenitor

characteristic: progenitor cell type

text: Homo sapiens

text: male

text: CK + DCI Day 1


E-MTAB-12960:CK + DCI Day 120

characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12960:CK + DCI Day 120

text: ArrayExpress

text: type II pneumocyte

text: human

text: fetal stage

text: normal

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: lung

text: lung epithelial progenitor

text: Homo sapiens

text: male

text: CK + DCI Day 120

characteristic: title


E-MTAB-12960:CK + DCI Day 21

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-12960:CK + DCI Day 21

characteristic: age

text: ArrayExpress

characteristic: broker name

text: type II pneumocyte

text: human

characteristic: common name

text: fetal stage

characteristic: developmental stage

text: normal

text: CK + DCI media organoid culture

text: Homo sapiens

text: Homo sapiens

text: lung

text: lung epithelial progenitor

text: Homo sapiens

text: female

text: CK + DCI Day 21


E-MTAB-12960:CK + DCI Day 6

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-12960:CK + DCI Day 6

text: ArrayExpress

text: type II pneumocyte

text: human

text: fetal stage

text: normal

characteristic: disease

text: CK + DCI media organoid culture

characteristic: growth condition

text: Homo sapiens

text: Homo sapiens

text: lung

characteristic: organism part

text: lung epithelial progenitor

text: Homo sapiens

text: male

characteristic: sex

text: CK + DCI Day 6


name: E-MTAB-12960:Lung Progenitor (Bud Tip) Organoids

characteristic: ENA-CHECKLIST

characteristic: INSDC first public

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: lung epithelial progenitor

text: human

characteristic: description

text: fetal stage

text: normal

text: Progenitor Media (3F)

text: Homo sapiens

text: Homo sapiens

text: lung

text: lung epithelial progenitor

characteristic: progenitor cell type

text: Homo sapiens

text: male

text: Lung Progenitor (Bud Tip) Organoids


E-MTAB-12960:Primary AT2 Organoid

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12960:Primary AT2 Organoid

unit: year

text: ArrayExpress

text: type II pneumocyte

text: human

text: adult

text: normal

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: lung

text: Homo sapiens

text: Primary AT2 Organoid

characteristic: title


characteristic: External Id

text: INRAE

characteristic: INSDC center name

text: public

text: broiler farm [ENVO:00000078]

characteristic: broad-scale environmental context

characteristic: collection date

text: Escherichia coli isolated from broiler feces at generation 2

text: feces [UBERON:0001988]

characteristic: environmental medium

text: France

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Nouzilly

characteristic: geographic location (region and locality)

characteristic: host age

text: broiler

characteristic: host common name

text: family 7446

characteristic: host family relationship

characteristic: host subject id

characteristic: host taxid

text: chicken fecal environment [ENVO:01001029]

characteristic: local environmental context

text: Escherichia coli

text: FluGenAvi: Environmental persistance of multidrug-resistant Enterobacteriales in a broiler experimental facility

characteristic: project name

text: Escherichia coli

text: E. coli isolate F1299


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: INRAE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: broiler farm [ENVO:00000078]

characteristic: description

text: feces [UBERON:0001988]

text: France

text: Nouzilly

text: broiler

text: family 7585

text: Escherichia coli

characteristic: organism

text: Escherichia coli

text: E. coli isolate F163

characteristic: title


characteristic: External Id

text: INRAE

characteristic: INSDC center name

text: public

text: broiler farm [ENVO:00000078]

characteristic: broad-scale environmental context

characteristic: collection date

text: feces [UBERON:0001988]

characteristic: environmental medium

text: France

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Nouzilly

characteristic: geographic location (region and locality)

characteristic: host age

text: broiler

characteristic: host common name

text: family 7585

text: Escherichia coli

text: Escherichia coli

text: E. coli isolate F167


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: INRAE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: broiler farm [ENVO:00000078]

text: feces [UBERON:0001988]

text: France

text: Nouzilly

text: broiler

text: family 7585

characteristic: host family relationship

characteristic: host subject id

characteristic: host taxid

characteristic: local environmental context

text: Escherichia coli

characteristic: project name

text: Escherichia coli

text: E. coli isolate F175


characteristic: External Id

text: INRAE

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: broiler farm [ENVO:00000078]

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: description

text: feces [UBERON:0001988]

characteristic: environmental medium

text: France

characteristic: geographic location (country and/or sea)

text: Nouzilly

text: broiler

text: family 7585

text: Escherichia coli

characteristic: organism

text: Escherichia coli

text: E. coli isolate F176

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: INRAE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: broiler farm [ENVO:00000078]

text: feces [UBERON:0001988]

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Nouzilly

characteristic: geographic location (region and locality)

characteristic: host age

text: broiler

characteristic: host common name

text: family 7654

text: Escherichia coli

text: Escherichia coli

text: E. coli isolate F262


characteristic: External Id

text: INRAE

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: broiler farm [ENVO:00000078]

characteristic: broad-scale environmental context

characteristic: collection date

text: feces [UBERON:0001988]

text: France

text: Nouzilly

text: broiler

text: family 7585

characteristic: host family relationship

characteristic: host subject id

characteristic: host taxid

characteristic: local environmental context

text: Escherichia coli

characteristic: project name

text: Escherichia coli

text: E. coli isolate F188


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: INRAE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: broiler farm [ENVO:00000078]

characteristic: description

text: feces [UBERON:0001988]

characteristic: environmental medium

text: France

characteristic: geographic location (country and/or sea)

text: Nouzilly

text: broiler

text: family 7585

text: Escherichia coli

characteristic: organism

text: Escherichia coli

text: E. coli isolate F189

characteristic: title


characteristic: External Id

text: INRAE

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: broiler farm [ENVO:00000078]

characteristic: broad-scale environmental context

characteristic: collection date

text: feces [UBERON:0001988]

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Nouzilly

characteristic: geographic location (region and locality)

characteristic: host age

text: broiler

characteristic: host common name

text: family 7585

text: Escherichia coli

text: Escherichia coli

text: E. coli isolate F190


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: INRAE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: broiler farm [ENVO:00000078]

text: feces [UBERON:0001988]

text: France

text: Nouzilly

text: broiler

text: family 7585

characteristic: host family relationship

characteristic: host subject id

characteristic: host taxid

characteristic: local environmental context

text: Escherichia coli

characteristic: project name

text: Escherichia coli

text: E. coli isolate F197


characteristic: External Id

text: INRAE

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: broiler farm [ENVO:00000078]

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: description

text: feces [UBERON:0001988]

characteristic: environmental medium

text: France

characteristic: geographic location (country and/or sea)

text: Nouzilly

text: broiler

text: family 7585

text: Escherichia coli

characteristic: organism

text: Escherichia coli

text: E. coli isolate F200

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: INRAE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: broiler farm [ENVO:00000078]

text: feces [UBERON:0001988]

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Nouzilly

characteristic: geographic location (region and locality)

characteristic: host age

text: broiler

characteristic: host common name

text: family 7585

text: Escherichia coli

text: Escherichia coli

text: E. coli isolate F202


characteristic: External Id

text: INRAE

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: broiler farm [ENVO:00000078]

characteristic: broad-scale environmental context

characteristic: collection date

text: feces [UBERON:0001988]

text: France

text: Nouzilly

text: broiler

text: family 7747

characteristic: host family relationship

characteristic: host subject id

characteristic: host taxid

characteristic: local environmental context

text: Escherichia coli

characteristic: project name

text: Escherichia coli

text: E. coli isolate F207


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: INRAE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: broiler farm [ENVO:00000078]

characteristic: description

text: feces [UBERON:0001988]

characteristic: environmental medium

text: France

characteristic: geographic location (country and/or sea)

text: Nouzilly

text: broiler

text: family 7722

text: Escherichia coli

characteristic: organism

text: Escherichia coli

text: E. coli isolate F247

characteristic: title


characteristic: External Id

text: INRAE

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: broiler farm [ENVO:00000078]

characteristic: broad-scale environmental context

characteristic: collection date

text: feces [UBERON:0001988]

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Nouzilly

characteristic: geographic location (region and locality)

characteristic: host age

text: broiler

characteristic: host common name

text: family 7722

text: Escherichia coli

text: Escherichia coli

text: E. coli isolate F248


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: INRAE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: broiler farm [ENVO:00000078]

text: feces [UBERON:0001988]

text: France

text: Nouzilly

text: broiler

text: family 7654

characteristic: host family relationship

characteristic: host subject id

characteristic: host taxid

characteristic: local environmental context

text: Escherichia coli

characteristic: project name

text: Escherichia coli

text: E. coli isolate F271


characteristic: External Id

text: INRAE

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: broiler farm [ENVO:00000078]

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: description

text: feces [UBERON:0001988]

characteristic: environmental medium

text: France

characteristic: geographic location (country and/or sea)

text: Nouzilly

text: broiler

text: family 7654

text: Escherichia coli

characteristic: organism

text: Escherichia coli

text: E. coli isolate F273

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: INRAE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: broiler farm [ENVO:00000078]

text: feces [UBERON:0001988]

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Nouzilly

characteristic: geographic location (region and locality)

characteristic: host age

text: broiler

characteristic: host common name

text: family 7446

text: Escherichia coli

text: Escherichia coli

text: E. coli isolate F279


characteristic: External Id

text: INRAE

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: broiler farm [ENVO:00000078]

characteristic: broad-scale environmental context

characteristic: collection date

text: feces [UBERON:0001988]

text: France

text: Nouzilly

text: broiler

text: family 7722

characteristic: host family relationship

characteristic: host subject id

characteristic: host taxid

characteristic: local environmental context

text: Escherichia coli

characteristic: project name

text: Escherichia coli

text: E. coli isolate F630


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: INRAE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: broiler farm [ENVO:00000078]

characteristic: description

text: feces [UBERON:0001988]

characteristic: environmental medium

text: France

characteristic: geographic location (country and/or sea)

text: Nouzilly

text: broiler

text: family 7722

text: Escherichia coli

characteristic: organism

text: Escherichia coli

text: E. coli isolate F637

characteristic: title


characteristic: External Id

text: INRAE

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: broiler farm [ENVO:00000078]

characteristic: broad-scale environmental context

characteristic: collection date

text: feces [UBERON:0001988]

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Nouzilly

characteristic: geographic location (region and locality)

characteristic: host age

text: broiler

characteristic: host common name

text: family 7722

text: Escherichia coli

text: Escherichia coli

text: E. coli isolate F641


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: INRAE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: broiler farm [ENVO:00000078]

text: feces [UBERON:0001988]

text: France

text: Nouzilly

text: broiler

text: family 7654

characteristic: host family relationship

characteristic: host subject id

characteristic: host taxid

characteristic: local environmental context

text: Escherichia coli

characteristic: project name

text: Escherichia coli

text: E. coli isolate F642


characteristic: External Id

text: INRAE

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: broiler farm [ENVO:00000078]

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: description

text: feces [UBERON:0001988]

characteristic: environmental medium

text: France

characteristic: geographic location (country and/or sea)

text: Nouzilly

text: broiler

text: family 7654

text: Escherichia coli

characteristic: organism

text: Escherichia coli

text: E. coli isolate F643

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: INRAE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: broiler farm [ENVO:00000078]

text: feces [UBERON:0001988]

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Nouzilly

characteristic: geographic location (region and locality)

characteristic: host age

text: broiler

characteristic: host common name

text: family 7654

text: Escherichia coli

text: Escherichia coli

text: E. coli isolate F644


characteristic: External Id

text: INRAE

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: broiler farm [ENVO:00000078]

characteristic: broad-scale environmental context

characteristic: collection date

text: feces [UBERON:0001988]

text: France

text: Nouzilly

text: broiler

text: family 7446

characteristic: host family relationship

characteristic: host subject id

characteristic: host taxid

characteristic: local environmental context

text: Escherichia coli

characteristic: project name

text: Escherichia coli

text: E. coli isolate F663


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: INRAE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: broiler farm [ENVO:00000078]

characteristic: description

text: feces [UBERON:0001988]

characteristic: environmental medium

text: France

characteristic: geographic location (country and/or sea)

text: Nouzilly

text: broiler

text: family 7446

text: Escherichia coli

characteristic: organism

text: Escherichia coli

text: E. coli isolate F664

characteristic: title


characteristic: External Id

text: INRAE

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: broiler farm [ENVO:00000078]

characteristic: broad-scale environmental context

characteristic: collection date

text: Escherichia coli isolated from broiler building surface at generation 3

text: concrete [ENVO:01000458]

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Nouzilly

characteristic: geographic location (region and locality)

text: cement floor [ENVO_01000489]

text: Escherichia coli

text: Escherichia coli

text: E. coli isolate S138


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: INRAE

characteristic: INSDC first public

text: public

text: broiler farm [ENVO:00000078]

text: concrete [ENVO:01000458]

text: France

text: Nouzilly

text: cement floor [ENVO_01000489]

characteristic: local environmental context

text: Escherichia coli

characteristic: project name

text: Escherichia coli

text: E. coli isolate S142


text: INRAE

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: broiler farm [ENVO:00000078]

characteristic: description

text: concrete [ENVO:01000458]

characteristic: environmental medium

text: France

characteristic: geographic location (country and/or sea)

text: Nouzilly

text: cement floor [ENVO_01000489]

text: Escherichia coli

characteristic: organism

text: Escherichia coli

text: E. coli isolate S143


characteristic: External Id

text: INRAE

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: broiler farm [ENVO:00000078]

characteristic: broad-scale environmental context

text: concrete [ENVO:01000458]

text: France

text: Nouzilly

text: cement floor [ENVO_01000489]

text: Escherichia coli

text: Escherichia coli

text: E. coli isolate S148

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: INRAE

text: public

text: broiler farm [ENVO:00000078]

characteristic: collection date

text: Escherichia coli isolated from broiler feces at generation 3

text: feces [UBERON:0001988]

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Nouzilly

characteristic: geographic location (region and locality)

characteristic: host age

text: broiler

characteristic: host common name

text: family 7722

characteristic: host family relationship

characteristic: host subject id

characteristic: host taxid

text: Escherichia coli

text: Escherichia coli

text: E. coli isolate F685


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRAE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: broiler farm [ENVO:00000078]

characteristic: description

text: feces [UBERON:0001988]

characteristic: environmental medium

text: France

characteristic: geographic location (country and/or sea)

text: Nouzilly

text: broiler

text: family 7654

characteristic: local environmental context

text: Escherichia coli

characteristic: organism

characteristic: project name

text: Escherichia coli

text: E. coli isolate F687


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: INRAE

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: broiler farm [ENVO:00000078]

characteristic: broad-scale environmental context

characteristic: collection date

text: feces [UBERON:0001988]

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Nouzilly

characteristic: geographic location (region and locality)

text: broiler

text: family 7654

text: Escherichia coli

text: Escherichia coli

text: E. coli isolate F688

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRAE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: broiler farm [ENVO:00000078]

characteristic: description

text: feces [UBERON:0001988]

text: France

text: Nouzilly

characteristic: host age

text: broiler

characteristic: host common name

text: family 7654

characteristic: host family relationship

characteristic: host subject id

characteristic: host taxid

text: Escherichia coli

text: Escherichia coli

text: E. coli isolate F692


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: INRAE

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: broiler farm [ENVO:00000078]

characteristic: broad-scale environmental context

text: feces [UBERON:0001988]

characteristic: environmental medium

text: France

characteristic: geographic location (country and/or sea)

text: Nouzilly

text: broiler

text: family 7722

characteristic: local environmental context

text: Escherichia coli

characteristic: organism

characteristic: project name

text: Escherichia coli

text: E. coli isolate F696


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRAE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: broiler farm [ENVO:00000078]

characteristic: collection date

characteristic: description

text: feces [UBERON:0001988]

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Nouzilly

characteristic: geographic location (region and locality)

text: broiler

text: family 7722

text: Escherichia coli

text: Escherichia coli

text: E. coli isolate F700

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: INRAE

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: broiler farm [ENVO:00000078]

characteristic: broad-scale environmental context

text: feces [UBERON:0001988]

text: France

text: Nouzilly

characteristic: host age

text: broiler

characteristic: host common name

text: family 7747

characteristic: host family relationship

characteristic: host subject id

characteristic: host taxid

text: Escherichia coli

text: Escherichia coli

text: E. coli isolate F704


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRAE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: broiler farm [ENVO:00000078]

characteristic: collection date

text: feces [UBERON:0001988]

characteristic: environmental medium

text: France

characteristic: geographic location (country and/or sea)

text: Nouzilly

text: broiler

text: family 7446

characteristic: local environmental context

text: Escherichia coli

characteristic: organism

characteristic: project name

text: Escherichia coli

text: E. coli isolate F719


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: INRAE

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: broiler farm [ENVO:00000078]

characteristic: broad-scale environmental context

characteristic: description

text: feces [UBERON:0001988]

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Nouzilly

characteristic: geographic location (region and locality)

text: broiler

text: family 7585

text: Escherichia coli

text: Escherichia coli

text: E. coli isolate F723

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRAE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: broiler farm [ENVO:00000078]

characteristic: collection date

text: feces [UBERON:0001988]

text: France

text: Nouzilly

characteristic: host age

text: broiler

characteristic: host common name

text: family 7585

characteristic: host family relationship

characteristic: host subject id

characteristic: host taxid

text: Escherichia coli

text: Escherichia coli

text: E. coli isolate F724


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: INRAE

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: broiler farm [ENVO:00000078]

characteristic: broad-scale environmental context

text: feces [UBERON:0001988]

characteristic: environmental medium

text: France

characteristic: geographic location (country and/or sea)

text: Nouzilly

text: broiler

text: family 7585

characteristic: local environmental context

text: Escherichia coli

characteristic: organism

characteristic: project name

text: Escherichia coli

text: E. coli isolate F728


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRAE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: broiler farm [ENVO:00000078]

characteristic: collection date

characteristic: description

text: feces [UBERON:0001988]

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Nouzilly

characteristic: geographic location (region and locality)

text: broiler

text: family 7585

text: Escherichia coli

text: Escherichia coli

text: E. coli isolate F730

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: INRAE

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: broiler farm [ENVO:00000078]

characteristic: broad-scale environmental context

text: feces [UBERON:0001988]

text: France

text: Nouzilly

characteristic: host age

text: broiler

characteristic: host common name

text: family 7722

characteristic: host family relationship

characteristic: host subject id

characteristic: host taxid

text: Escherichia coli

text: Escherichia coli

text: E. coli isolate F766


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRAE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: broiler farm [ENVO:00000078]

characteristic: collection date

text: feces [UBERON:0001988]

characteristic: environmental medium

text: France

characteristic: geographic location (country and/or sea)

text: Nouzilly

text: broiler

text: family 7654

characteristic: local environmental context

text: Escherichia coli

characteristic: organism

characteristic: project name

text: Escherichia coli

text: E. coli isolate F785


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: INRAE

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: broiler farm [ENVO:00000078]

characteristic: broad-scale environmental context

characteristic: description

text: feces [UBERON:0001988]

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Nouzilly

characteristic: geographic location (region and locality)

text: broiler

text: family 7654

text: Escherichia coli

text: Escherichia coli

text: E. coli isolate F787

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRAE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: broiler farm [ENVO:00000078]

characteristic: collection date

text: feces [UBERON:0001988]

text: France

text: Nouzilly

characteristic: host age

text: broiler

characteristic: host common name

text: family 7722

characteristic: host family relationship

characteristic: host subject id

characteristic: host taxid

text: Escherichia coli

text: Escherichia coli

text: E. coli isolate F794


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: INRAE

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: broiler farm [ENVO:00000078]

characteristic: broad-scale environmental context

text: feces [UBERON:0001988]

characteristic: environmental medium

text: France

characteristic: geographic location (country and/or sea)

text: Nouzilly

text: broiler

text: family 7747

characteristic: local environmental context

text: Escherichia coli

characteristic: organism

characteristic: project name

text: Escherichia coli

text: E. coli isolate F801


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRAE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: broiler farm [ENVO:00000078]

characteristic: collection date

characteristic: description

text: feces [UBERON:0001988]

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Nouzilly

characteristic: geographic location (region and locality)

text: broiler

text: family 7446

text: Escherichia coli

text: Escherichia coli

text: E. coli isolate F829

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: INRAE

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: broiler farm [ENVO:00000078]

characteristic: broad-scale environmental context

text: feces [UBERON:0001988]

text: France

text: Nouzilly

characteristic: host age

text: broiler

characteristic: host common name

text: family 7585

characteristic: host family relationship

characteristic: host subject id

characteristic: host taxid

text: Escherichia coli

text: Escherichia coli

text: E. coli isolate F841


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRAE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: broiler farm [ENVO:00000078]

characteristic: collection date

text: feces [UBERON:0001988]

characteristic: environmental medium

text: France

characteristic: geographic location (country and/or sea)

text: Nouzilly

text: broiler

text: family 7585

characteristic: local environmental context

text: Escherichia coli

characteristic: organism

characteristic: project name

text: Escherichia coli

text: E. coli isolate F845


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: INRAE

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: broiler farm [ENVO:00000078]

characteristic: broad-scale environmental context

characteristic: description

text: feces [UBERON:0001988]

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Nouzilly

characteristic: geographic location (region and locality)

text: broiler

text: family 7585

text: Escherichia coli

text: Escherichia coli

text: E. coli isolate F846

characteristic: title