characteristic: ENA-CHECKLIST

text: Robert Koch Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Random sampling

text: IMS-10267-CVDP-565BF025-F7D9-46B4-81FE-EAFCEE7E43F5

text: RKI-OSEDA

text: not provided

characteristic: collector name

text: Germany

characteristic: geographic location (country and/or sea)

text: Europe/Germany/Saxony

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided


status: PUBLIC

tag: attribute

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Robert Koch Institute

characteristic: INSDC center name

text: public

characteristic: SRA accession

text: Random sampling

characteristic: Sampling Strategy

text: IMS-10267-CVDP-A4E20545-1BDB-4E30-BA5B-CDF7A61229B4

tag: Namespace:Robert Koch Institute

characteristic: Submitter Id

text: RKI-OSEDA

characteristic: broker name

text: labopart - Medizinische Laboratorien Dresden, Wurzener Str. 5; 01127 Dresden

characteristic: collecting institution

characteristic: collection date

text: not provided

text: Germany

text: Europe/Germany/Saxony

text: Human

text: not provided

text: Homo sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: Severe acute respiratory syndrome coronavirus 2

characteristic: organism

text: active surveillance in response to outbreak

characteristic: sample capture status

text: Molecular surveillance of SARS-CoV-2 in Germany

characteristic: title


characteristic: ENA-CHECKLIST

text: Robert Koch Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Random sampling

text: IMS-10267-CVDP-F4BD4EF6-523F-4FE3-8B96-3DEF4F160DCF

text: RKI-OSEDA

text: not provided

characteristic: collector name

text: Germany

characteristic: geographic location (country and/or sea)

text: Europe/Germany/Saxony

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Robert Koch Institute

characteristic: INSDC center name

text: public

characteristic: SRA accession

text: Random sampling

characteristic: Sampling Strategy

text: IMS-10267-CVDP-2F40BA03-F49C-4A59-A1E6-A7F062DAD1C9

characteristic: Submitter Id

text: RKI-OSEDA

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

text: Germany

text: Europe/Germany/Saxony

text: Human

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided


characteristic: ENA-CHECKLIST

text: Robert Koch Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Random sampling

text: IMS-10013-CVDP-97069FBD-AFE8-4937-8050-47CCCB35E213

text: RKI-OSEDA

text: LabKom - Labor an der Salzbrücke MVZ GmbH, An der Salzbrücke 3; 98617 Ritschenhausen

text: not provided

text: Germany

text: Europe/Germany/Thuringia

text: Human

text: not provided

text: Homo sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: organism

characteristic: sample capture status

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Robert Koch Institute

characteristic: INSDC center name

text: public

text: Random sampling

text: IMS-10013-CVDP-E246246F-720B-4C2F-B3BD-8A5F9070AFA8

text: RKI-OSEDA

text: not provided

characteristic: collector name

text: Germany

characteristic: geographic location (country and/or sea)

text: Europe/Germany/Thuringia

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided


characteristic: ENA-CHECKLIST

text: Robert Koch Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Random sampling

characteristic: Sampling Strategy

text: IMS-10013-CVDP-C51E1872-5BA5-4050-96DD-781F112C48B4

characteristic: Submitter Id

text: RKI-OSEDA

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

text: Germany

text: Europe/Germany/Thuringia

text: Human

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Robert Koch Institute

characteristic: INSDC center name

text: public

text: Random sampling

text: IMS-10013-CVDP-AA4F07D5-B062-44FB-8784-C4E00927224F

text: RKI-OSEDA

text: not provided

text: Germany

text: Europe/Germany/Thuringia

text: Human

text: not provided

text: Homo sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: organism

characteristic: sample capture status

characteristic: title


characteristic: ENA-CHECKLIST

text: Robert Koch Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Random sampling

text: IMS-10013-CVDP-57D453C0-9D50-4389-9CAA-639AD8404E62

text: RKI-OSEDA

text: not provided

characteristic: collector name

text: Germany

characteristic: geographic location (country and/or sea)

text: Europe/Germany/Thuringia

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Robert Koch Institute

characteristic: INSDC center name

text: public

characteristic: SRA accession

text: Random sampling

characteristic: Sampling Strategy

text: IMS-10013-CVDP-BAFA7547-7632-4C17-AB1B-59DDEB19240F

characteristic: Submitter Id

text: RKI-OSEDA

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

text: Germany

text: Europe/Germany/Thuringia

text: Human

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided


characteristic: ENA-CHECKLIST

text: Robert Koch Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Random sampling

text: IMS-10013-CVDP-F6AC13DD-1167-4ED3-96D6-147D31BC8AA1

text: RKI-OSEDA

text: not provided

text: Germany

text: Europe/Germany/Thuringia

text: Human

text: not provided

text: Homo sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: organism

characteristic: sample capture status

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Robert Koch Institute

characteristic: INSDC center name

text: public

text: Random sampling

text: IMS-10013-CVDP-9684DDA3-05E2-4DA9-B3D8-D9F3BF241F68

text: RKI-OSEDA

text: not provided

characteristic: collector name

text: Germany

characteristic: geographic location (country and/or sea)

text: Europe/Germany/Thuringia

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided


characteristic: ENA-CHECKLIST

text: Robert Koch Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Random sampling

characteristic: Sampling Strategy

text: IMS-10013-CVDP-6851C4C7-C297-468C-9D42-F0B7FDD78413

characteristic: Submitter Id

text: RKI-OSEDA

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

text: Germany

text: Europe/Germany/Thuringia

text: Human

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Robert Koch Institute

characteristic: INSDC center name

text: public

text: Random sampling

text: IMS-10013-CVDP-90A5EF0D-DB1B-4FD9-B4E3-593A8580D0D9

text: RKI-OSEDA

text: not provided

text: Germany

text: Europe/Germany/Thuringia

text: Human

text: not provided

text: Homo sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: organism

characteristic: sample capture status

characteristic: title


characteristic: ENA-CHECKLIST

text: Robert Koch Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Random sampling

text: IMS-10013-CVDP-33B9120C-782C-4AF4-9D7D-F69EC6636E36

text: RKI-OSEDA

text: not provided

characteristic: collector name

text: Germany

characteristic: geographic location (country and/or sea)

text: Europe/Germany/Thuringia

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Robert Koch Institute

characteristic: INSDC center name

text: public

characteristic: SRA accession

text: Random sampling

characteristic: Sampling Strategy

text: IMS-10013-CVDP-E367251D-6A95-41BD-A68C-12E4A16903A8

characteristic: Submitter Id

text: RKI-OSEDA

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

text: Germany

text: Europe/Germany/Thuringia

text: Human

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided

text: Severe acute respiratory syndrome coronavirus 2


characteristic: ENA-CHECKLIST

text: Robert Koch Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Random sampling

text: IMS-10285-CVDP-CCAD1D09-161D-4C90-971F-1BC74968CB8A

text: RKI-OSEDA

text: Dianovis GmbH Greiz, Wichmannstraße 12; 07973 Greiz

text: not provided

text: Germany

text: Europe/Germany/Thuringia

text: Human

text: not provided

text: Homo sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: organism

text: active surveillance in response to outbreak

characteristic: sample capture status

text: Molecular surveillance of SARS-CoV-2 in Germany

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Robert Koch Institute

characteristic: INSDC center name

text: public

text: Random sampling

text: IMS-10285-CVDP-51E7AB82-D940-4AD9-8D8F-163AD2EDD537

text: RKI-OSEDA

text: not provided

characteristic: collector name

text: Germany

characteristic: geographic location (country and/or sea)

text: Europe/Germany/Thuringia

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided


characteristic: ENA-CHECKLIST

text: Robert Koch Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Random sampling

characteristic: Sampling Strategy

text: IMS-10285-CVDP-0EEB51E0-DC28-411E-9509-62D09E5456E5

characteristic: Submitter Id

text: RKI-OSEDA

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

text: Germany

text: Europe/Germany/Thuringia

text: Human

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Robert Koch Institute

characteristic: INSDC center name

text: public

text: Random sampling

text: IMS-10285-CVDP-B8157085-394D-4C6E-BD78-3E3A90DBA681

text: RKI-OSEDA

text: not provided

text: Germany

text: Europe/Germany/Thuringia

text: Human

text: not provided

text: Homo sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: organism

characteristic: sample capture status

characteristic: title


characteristic: ENA-CHECKLIST

text: Robert Koch Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Random sampling

text: IMS-10013-CVDP-58E78AC5-E3E7-4BF6-AE03-79126ABC0FAF

text: RKI-OSEDA

text: LabKom - Labor an der Salzbrücke MVZ GmbH, An der Salzbrücke 3; 98617 Ritschenhausen

text: not provided

characteristic: collector name

text: Germany

characteristic: geographic location (country and/or sea)

text: Europe/Germany/Thuringia

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Robert Koch Institute

characteristic: INSDC center name

text: public

characteristic: SRA accession

text: Random sampling

characteristic: Sampling Strategy

text: IMS-10013-CVDP-F653F87C-DDBC-4CC5-92F0-B0486638B109

characteristic: Submitter Id

text: RKI-OSEDA

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

text: Germany

text: Europe/Germany/Thuringia

text: Human

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided


characteristic: ENA-CHECKLIST

text: Robert Koch Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Random sampling

text: IMS-10285-CVDP-83D3EF2C-08BB-4CA1-A681-7A77095CDB12

text: RKI-OSEDA

text: not provided

text: Germany

text: Europe/Germany/Thuringia

text: Human

text: not provided

text: Homo sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: organism

characteristic: sample capture status

characteristic: title


E-MTAB-13093:Sample 1

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-13093:Sample 1

tag: Namespace:1Institute of Cardiovascular Regeneration, Centre for Molecular Medicine, Goethe University Frankfurt, Theodor Stern Kai 7, 60590 Frankfurt, Germany.

unit: month

characteristic: age

text: ArrayExpress

text: endothelial cell

text: not collected

text: house mouse

characteristic: common name

text: Protocols: To eliminate senescent cells from aged mice, a combination of the two senolytics drugs dasatinib and quercetin was used as proposed by Xu et al.(37). In brief, 5 mg/kg dasatinib (SML2589-50MG; Merck) and 50 mg/kg quercetin (Q4951-10G, Sigma-Aldrich) were applied via oral gavage to aged mice (18 months) on three consecutive days, every second week for a total duration of two months. Young (3 to 4 months) and aged (18 to 19 months) mice receiving the solvent (Phosal 50PG (368315, Lipoid) containing 3.3% ethanol (32221, Sigma-Aldrich) and 10% polyethylene glycol 400 (807485, Merck)) served as control cohorts. Cardiac function was monitored during the experiment using echocardiography and ECG traces as described below. The animal experiment has been conducted as approved by the state of Hessen. For Mouse hearts, the following protocol was used: cardiac tissues were thawed on ice and cut into small pieces. Minced tissue was pre-digested with a 5-ml enzyme solution of collagenase (2,500 U, Thermo Fisher Scientific) in HBSS+/+ (Gibco) for 10 min at 37 °C in a water bath. After centrifugation at 500g and 4 °C for 5 min, the supernatant was discarded, and nuclei were isolated after cell disruption with a glass dounce homogenizer (five strokes with a loose pestle and ten strokes with a tight pestle)40,41. After filtering (20-µm strainer, pluriSelect), the suspension was centrifuged at 1,000g and 4 °C for 6 min and resuspended in 500 µl staining buffer containing 1% BSA (Sigma-Aldrich), 5 nM MgCl2 (Sigma-Aldrich), 1 mM EDTA (Gibco), 1 mM EGTA (Gibco), 0.2 U µl−1 RNasin Plus Inhibitor (Promega) and 0.1 µg ml−1 Hoechst (Life Technologies) in Dulbecco´s phosphaste buffered saline (DPBS). Hoechst-positive nuclei were separated from cell debris by using the FACSAria Fusion instrument (BD Biosciences) and sorted into staining buffer without Hoechst at 4 °C. Nuclear suspensions were loaded on a 10x Chromium Controller (10x Genomics) according to the manufacturer’s protocol. All snRNA-seq libraries were prepared using the Chromium Single Cell 3′ version 3 Reagent kit (10x Genomics) according to the manufacturer’s protocol. Individual nuclei were isolated into droplets together with gel beads coated with unique primers bearing 10x cell barcodes, UMIs and poly(dT) sequences. Reverse transcription reactions generated barcoded full-length cDNA followed by disruption of emulsions using the recovery agent and cDNA cleanup with DynaBeads MyOne Silane Beads (Thermo Fisher Scientific). Total cDNA was amplified using a Biometra Thermocycler TProfessional Basic Gradient with a 96-well sample block (98 °C for 3 min; cycled 14×: 98 °C for 15 s, 67 °C for 20 s and 72 °C for 1 min; 72 °C for 1 min; held at 4 °C). Amplified cDNA products were cleaned with the SPRIselect Reagent kit (Beckman Coulter). Indexed sequencing libraries were constructed using reagents from the Chromium Single Cell 3′ version 3 Reagent kit as follows: fragmentation, end repair and A tailing; size selection with SPRIselect; adaptor ligation; post-ligation cleanup with SPRIselect; sample index PCR and cleanup with SPRIselect beads. Library quantification and quality assessment were performed using the Bioanalyzer Agilent 2100 with a High Sensitivity DNA chip (Agilent Genomics). Indexed libraries were pooled equimolarly and sequenced on the Illumina NovaSeq 6000 by GenomeScan using paired-end 26 × 98-bp reads as the sequencing mode.

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

text: Mus musculus

text: Mus musculus

text: heart

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex

characteristic: strain

text: Sample 1


E-MTAB-13093:Sample 2

characteristic: ENA-CHECKLIST

text: 1Institute of Cardiovascular Regeneration, Centre for Molecular Medicine, Goethe University Frankfurt, Theodor Stern Kai 7, 60590 Frankfurt, Germany.

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13093:Sample 2

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: endothelial cell

text: not collected

characteristic: collection date

text: house mouse

text: adult

text: wild type genotype

text: not collected

text: not applicable

characteristic: isolate

text: Mus musculus

characteristic: organism

text: Mus musculus

text: heart

text: Mus musculus

text: male

text: Sample 2


E-MTAB-13093:Sample 3

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-13093:Sample 3

characteristic: age

text: ArrayExpress

text: endothelial cell

text: not collected

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

text: not collected

text: not applicable

text: Mus musculus

text: Mus musculus

text: heart

text: Mus musculus

text: male

text: Sample 3

characteristic: title


E-MTAB-13093:Sample 4

characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-13093:Sample 4

text: ArrayExpress

text: endothelial cell

text: not collected

text: house mouse

text: adult

text: wild type genotype

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

text: Mus musculus

text: Mus musculus

text: heart

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex

characteristic: strain

text: Sample 4


E-MTAB-13093:Sample 5

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13093:Sample 5

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: endothelial cell

text: not collected

characteristic: collection date

text: house mouse

text: adult

text: wild type genotype

text: not collected

text: not applicable

characteristic: isolate

text: Mus musculus

characteristic: organism

text: Mus musculus

text: heart

text: Mus musculus

text: male

text: Sample 5


E-MTAB-13093:Sample 6

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-13093:Sample 6

characteristic: age

text: ArrayExpress

text: endothelial cell

text: not collected

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

text: not collected

text: not applicable

text: Mus musculus

text: Mus musculus

text: heart

text: Mus musculus

text: male

text: Sample 6

characteristic: title


E-MTAB-13093:Sample 7

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

text: E-MTAB-13093:Sample 7

text: ArrayExpress

text: endothelial cell

text: not collected

text: house mouse

text: adult

text: wild type genotype

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

text: Mus musculus

text: Mus musculus

text: heart

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex

characteristic: strain

text: Sample 7


characteristic: ENA-CHECKLIST

characteristic: External Id

text: JOHANNES GUTENBERG UNIVERSITY MAINZ

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:JOHANNES GUTENBERG UNIVERSITY MAINZ

characteristic: Submitter Id

characteristic: collection date

text: human

text: Homo sapiens bone sample

text: Russia

text: Homo sapiens

characteristic: organism

text: Homo sapiens


characteristic: INSDC center name

characteristic: INSDC first public

text: public

tag: Namespace:Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium VIB Center for Plant Systems Biology, 9052 Ghent, Belgium

unit: day

characteristic: age

text: ArrayExpress

characteristic: broker name

text: not collected

text: Japanese rice

characteristic: common name

text: Nipponbare

characteristic: cultivar

text: Protocols: root sections between 750 µm and 2000 µm from the root tip were microdissected Root parts (750 um - 2000 um from the root tip) were dissected with razor blades. Rice seeds were germinated in Petri dishes on three layers of wetted whatman paper saturated with a 5 µM N-1-naphthylphthalamic acid (NPA) watery solution for two days at 28°C and uniformly grown seedlings were transferred to 250 mL-Erlenmeyer flasks containing 50 mL of liquid half strength Murashige and Skoog medium (MS1/2) supplemented with 5 µM NPA. Flasks were put on a shaker (120 rpm) in a growth room at 24 °C, 55 μE/m−2 s−1 light intensity, and 16 h/8 h day/night regime for 3 days. For treatment, the medium in the flasks was replaced by MS1/2 supplemented with 5 µM 1-naphthalene acetic acid (NAA) or NPA as a control. In a reverse transcription reaction using random primers, RNA was converted into first strand cDNA and subsequently converted into double-stranded cDNA in a second strand cDNA synthesis reaction using DNA PolymeraseI and RNAse H. The cDNA fragments were extended with a single 'A' base to the 3' ends of the blunt-ended cDNA fragments after which indexing adapters were ligated introducing single distinct barcodes for each sample. Finally, enrichment PCR was carried out to enrich those DNA fragments that have adapter molecules on both ends and to amplify the amount of DNA in the library. total RNA was extracted using Trizol (Invitrogen) and the RNeasy Plant mini kit (Qiagen) Using the Illumina TruSeq® Stranded mRNA Sample Prep Kit, poly-A containing mRNA molecules were purified from the total RNA input using poly-T oligo-attached magnetic beads. In a reverse transcription reaction using random primers, RNA was converted into first strand cDNA and subsequently converted into double-stranded cDNA in a second strand cDNA synthesis reaction using DNA PolymeraseI and RNAse H. The cDNA fragments were extended with a single 'A' base to the 3' ends of the blunt-ended cDNA fragments after which indexing adapters were ligated introducing single distinct barcodes for each sample. Finally, enrichment PCR was carried out to enrich those DNA fragments that have adapter molecules on both ends and to amplify the amount of DNA in the library.

characteristic: description

text: seedling development stage

characteristic: developmental stage

text: wild type genotype

text: not collected

text: not applicable

characteristic: isolate

text: Oryza sativa Japonica Group

text: Oryza sativa Japonica Group

text: root

text: root sections (750 µm - 2000 µm from the root tip)

characteristic: sampling site

text: Oryza sativa Japonica Group

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium VIB Center for Plant Systems Biology, 9052 Ghent, Belgium

characteristic: INSDC last update

text: public

text: ArrayExpress

text: not collected

text: Japanese rice

text: Nipponbare

text: seedling development stage

text: wild type genotype

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

text: Oryza sativa Japonica Group

text: Oryza sativa Japonica Group

text: root

characteristic: organism part

text: Oryza sativa Japonica Group


text: Swedish Univ. Agric. Sci.

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Swedish Univ. Agric. Sci.

characteristic: Submitter Id

text: Outdoor growth

characteristic: air temperature regimen

text: Tuber cross-sections

characteristic: collection date

text: potato

text: Bintje (de Vries, 1910)

text: Tubers were collected from potato plants grown outdoors in 15L pots for 20 weeks. Four tubers were cut in 5mm cross sections and pooled into one sample.

text: Mature tubers

text: 840 Mb

characteristic: estimated size

text: Sweden

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Uppsala

characteristic: geographic location (region and locality)

text: experimental garden

characteristic: growth facility

text: Tuber cross section

text: dark

characteristic: light regimen

text: Common practice

characteristic: mineral nutrient regimen

text: Solanum tuberosum

characteristic: organism

text: Potato

characteristic: organism common name

text: End of the tuber maturation stage

characteristic: plant developmental stage

text: Commercial soil mixture (Hammenhög AB)

characteristic: plant growth medium

text: subterranean shoot axis tuber

characteristic: plant structure

text: Control tubers

characteristic: plant treatment

text: Tetraploid

characteristic: ploidy

text: Asexual

characteristic: propagation

text: 15L black plastic pots, randomly distributed in an outdoor growth facility

characteristic: rooting conditions

text: healthy

characteristic: sample health state

text: Solanum tuberosum

text: Bj-control (Co)

text: Common practice

characteristic: watering regimen


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Swedish Univ. Agric. Sci.

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Outdoor growth

characteristic: air temperature regimen

text: Tuber cross-sections

characteristic: collection date

text: potato

characteristic: common name

text: Bintje (de Vries, 1910)

characteristic: cultivar

text: Tubers were collected from potato plants grown outdoors in 15L pots for 20 weeks. Four tubers were incubated in a growth cabinet with 100µE light from fluorescent light tubes. After 48h, tubers were cut in 5mm cross sections and pooled into one sample.

characteristic: description

text: Mature tubers

text: 840 Mb

characteristic: estimated size

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Uppsala

text: experimental garden

text: Tuber cross section

text: 100µE white light from fluorescent tubes (Petersson et al JAFC 2013)

text: Common practice

text: Solanum tuberosum

text: Potato

text: End of the tuber maturation stage

text: Commercial soil mixture (Hammenhög AB)

text: subterranean shoot axis tuber

text: Light exposed tubers

text: Tetraploid

text: Asexual

text: healthy

text: Solanum tuberosum

text: Bj-light (L48)

characteristic: title

text: Common practice


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Swedish Univ. Agric. Sci.

text: public

text: Outdoor growth

text: Tuber cross-sections

text: potato

text: Bintje (de Vries, 1910)

text: Tubers were collected from potato plants grown outdoors in 15L pots for 20 weeks. Four tubers were cut in 5mm cross sections, incubated in a petri dish kept in darkness for 24h, and pooled into one sample.

text: Mature tubers

text: 840 Mb

text: Sweden

characteristic: geographic location (longitude)

text: Uppsala

characteristic: geographic location (region and locality)

text: experimental garden

characteristic: growth facility

text: Tuber cross section

text: dark

characteristic: light regimen

text: Common practice

characteristic: mineral nutrient regimen

text: Solanum tuberosum

characteristic: organism

text: Potato

characteristic: organism common name

text: End of the tuber maturation stage

characteristic: plant developmental stage

text: Commercial soil mixture (Hammenhög AB)

characteristic: plant growth medium

text: subterranean shoot axis tuber

characteristic: plant structure

text: Wounded tubers (slicing)

characteristic: plant treatment

text: Tetraploid

characteristic: ploidy

text: Asexual

characteristic: propagation

characteristic: rooting conditions

text: healthy

characteristic: sample health state

text: Solanum tuberosum

text: Bj-wound (W24)

text: Common practice

characteristic: watering regimen


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Swedish Univ. Agric. Sci.

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Outdoor growth

characteristic: air temperature regimen

text: Tuber cross-sections

characteristic: collection date

text: potato

characteristic: common name

text: Magnum Bonum (J. Clark, 1876)

characteristic: cultivar

characteristic: description

text: Mature tubers

text: 840 Mb

characteristic: estimated size

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Uppsala

text: experimental garden

text: Tuber cross section

text: dark

text: Common practice

text: Solanum tuberosum

text: Potato

text: End of the tuber maturation stage

text: Commercial soil mixture (Hammenhög AB)

text: subterranean shoot axis tuber

text: Control tubers

text: Tetraploid

text: Asexual

text: healthy

text: Solanum tuberosum

text: MB-control (Co)

characteristic: title

text: Common practice


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Swedish Univ. Agric. Sci.

text: public

text: Outdoor growth

text: Tuber cross-sections

text: potato

text: Magnum Bonum (J. Clark, 1876)

text: Mature tubers

text: 840 Mb

text: Sweden

characteristic: geographic location (longitude)

text: Uppsala

characteristic: geographic location (region and locality)

text: experimental garden

characteristic: growth facility

text: Tuber cross section

characteristic: light regimen

text: Common practice

characteristic: mineral nutrient regimen

text: Solanum tuberosum

characteristic: organism

text: Potato

characteristic: organism common name

text: End of the tuber maturation stage

characteristic: plant developmental stage

text: Commercial soil mixture (Hammenhög AB)

characteristic: plant growth medium

text: subterranean shoot axis tuber

characteristic: plant structure

text: Light exposed tubers

characteristic: plant treatment

text: Tetraploid

characteristic: ploidy

text: Asexual

characteristic: propagation

characteristic: rooting conditions

text: healthy

characteristic: sample health state

text: Solanum tuberosum

text: MB-light (L48)

text: Common practice

characteristic: watering regimen


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Swedish Univ. Agric. Sci.

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Outdoor growth

characteristic: air temperature regimen

text: Tuber cross-sections

characteristic: collection date

text: potato

characteristic: common name

text: Magnum Bonum (J. Clark, 1876)

characteristic: cultivar

characteristic: description

text: Mature tubers

text: 840 Mb

characteristic: estimated size

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Uppsala

text: experimental garden

text: Tuber cross section

text: dark

text: Common practice

text: Solanum tuberosum

text: Potato

text: End of the tuber maturation stage

text: Commercial soil mixture (Hammenhög AB)

text: subterranean shoot axis tuber

text: Wounded tubers (slicing)

text: Tetraploid

text: Asexual

text: healthy

text: Solanum tuberosum

text: MB-wound (W24)

characteristic: title

text: Common practice


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: LBMC UMR5239

text: public

text: ena-SAMPLE-TAB-20-06-2023-15:42:23:173-29568

tag: Namespace:LBMC UMR5239

text: synchronized pooled L1 larvae

text: France

text: Mesorhabditis belari

characteristic: organism

text: Mesorhabditis belari

text: mixed

characteristic: sex

characteristic: strain


characteristic: External Id

text: LBMC UMR5239

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-20-06-2023-15:42:23:173-29569

text: single L1 larvae

text: France

text: Mesorhabditis belari

text: Mesorhabditis belari

text: mixed


text: LBMC UMR5239

text: public

text: ena-SAMPLE-TAB-20-06-2023-15:42:23:173-29570

characteristic: Submitter Id

characteristic: collection date

text: single L1 larvae

characteristic: description

text: France

text: Mesorhabditis belari

text: Mesorhabditis belari

text: mixed


text: LBMC UMR5239

text: public

text: ena-SAMPLE-TAB-20-06-2023-15:42:23:173-29571

text: single L1 larvae

text: France

characteristic: geographic location (country and/or sea)

text: Mesorhabditis belari

text: Mesorhabditis belari

text: mixed


text: LBMC UMR5239

text: public

text: ena-SAMPLE-TAB-20-06-2023-15:42:23:173-29572

text: single L1 larvae

text: France

text: Mesorhabditis belari

text: Mesorhabditis belari

text: mixed

characteristic: title


characteristic: ENA-CHECKLIST

text: LBMC UMR5239

text: public

text: ena-SAMPLE-TAB-20-06-2023-15:42:23:173-29573

text: single L1 larvae

text: France

text: Mesorhabditis belari

text: Mesorhabditis belari

text: mixed


characteristic: ENA-FIRST-PUBLIC

text: LBMC UMR5239

text: public

text: ena-SAMPLE-TAB-20-06-2023-15:42:23:173-29574

text: synchronized pooled L1 larvae

text: France

text: Mesorhabditis spiculigera

text: Mesorhabditis spiculigera

text: mixed


characteristic: ENA-LAST-UPDATE

text: LBMC UMR5239

text: public

text: manually selected adult males

text: adult

text: France

text: Mesorhabditis belari

text: Mesorhabditis belari

text: male


text: LBMC UMR5239

text: public

text: pooled mixed stages

text: mixed

text: France

text: Mesorhabditis spiculigera

characteristic: organism

text: Mesorhabditis spiculigera

text: mixed


text: LBMC UMR5239

text: public

text: manually selected adult males

text: adult

text: France

text: Mesorhabditis spiculigera

text: Mesorhabditis spiculigera

text: male

characteristic: sex


text: LBMC UMR5239

text: public

text: manually selected adult females

text: adult

text: France

text: Mesorhabditis spiculigera

text: Mesorhabditis spiculigera

text: female

characteristic: strain


characteristic: External Id

text: LBMC UMR5239

text: public

text: manually selected adult females

text: adult

text: France

text: Mesorhabditis belari

text: Mesorhabditis belari

text: female


text: LBMC UMR5239

characteristic: INSDC center name

text: public

text: single dissected adult female gonad

text: adult

text: France

text: Mesorhabditis belari

text: Mesorhabditis belari

text: female


text: LBMC UMR5239

characteristic: INSDC first public

text: public

text: single dissected adult male gonad

text: adult

text: France

text: Mesorhabditis belari

text: Mesorhabditis belari

text: male


text: UNIVERSITY OF CAPE TOWN

characteristic: INSDC last update

text: public

tag: Namespace:UNIVERSITY OF CAPE TOWN

text: human

characteristic: common name

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: UNIVERSITY OF CAPE TOWN

text: public

characteristic: INSDC status

characteristic: SRA accession

text: human

text: Family1,Maternal Aunt,Progressor

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: UNIVERSITY OF CAPE TOWN

text: public

characteristic: Submitter Id

characteristic: collection date

text: human

text: Family1,Maternal Uncle,Progressor

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: UNIVERSITY OF CAPE TOWN

text: public

text: human

text: Family10,Maternal Grandmother,Progressor

characteristic: description

text: South Africa

characteristic: geographic location (country and/or sea)

text: Homo sapiens

text: Homo sapiens


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAPE TOWN

text: public

text: human

text: Family10,Maternal Aunt,Progressor

text: South Africa

text: Homo sapiens

text: Homo sapiens

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF CAPE TOWN

text: public

text: human

text: Family20,Maternal Grandfather,Negative

text: South Africa

text: Homo sapiens

text: Homo sapiens


109 bk Tn-seq LA5

text: INRAE

text: public

text: 109 bk Tn-seq LA5

text: a saturated library in Salmonella Enteritidis virulent strain

text: France

text: not applicable

characteristic: host health state

text: Salmonella enterica subsp. enterica serovar Enteritidis str. LA5

characteristic: host scientific name

text: INRAE Val de Loire Research Center Unit Infectiology andPublic Health (ISP BAT311) route de Crotelles 37380 Nouzilly France

characteristic: isolate

text: INRAE Val de Loire Research Center Unit Infectiology and Public Health (ISP BAT311) route de Crotelles 37380 Nouzilly France

characteristic: organism

text: O 1,9,12 H g,m

characteristic: serovar

text: Tnseq Library of Salmonella Enteritidis str LA5


characteristic: External Id

text: AZTI Tecnalia

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

tag: Namespace:AZTI Tecnalia

text: Marine biome

characteristic: broad-scale environmental context

unit: m

characteristic: depth

text: Pelagic ARMS-1 2mm pore size

characteristic: elevation

text: ARMS

characteristic: environmental medium

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Pelagic biome

characteristic: local environmental context

text: metagenome

text: COI metabarcoding study of pelagic ARMS communities deployed in Mendexa, Basque Country

characteristic: project name

text: metagenome


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: AZTI Tecnalia

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Marine biome

characteristic: collection date

text: Pelagic ARMS-1 1mm pore size

characteristic: description

text: ARMS

text: Spain

characteristic: geographic location (country and/or sea)

text: Pelagic biome

text: metagenome

text: metagenome

characteristic: title


characteristic: External Id

text: AZTI Tecnalia

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Marine biome

characteristic: broad-scale environmental context

characteristic: depth

text: Pelagic ARMS-1 500uM pore size

text: ARMS

text: Spain

text: Pelagic biome

text: metagenome

characteristic: organism

text: metagenome


characteristic: ENA-CHECKLIST

text: AZTI Tecnalia

text: public

text: Marine biome

text: Pelagic ARMS-1 100uM pore size

characteristic: elevation

text: ARMS

characteristic: environmental medium

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Pelagic biome

characteristic: local environmental context

text: metagenome

characteristic: project name

text: metagenome


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: AZTI Tecnalia

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Marine biome

characteristic: collection date

text: Pelagic ARMS-1 sessile

characteristic: description

text: ARMS

text: Spain

text: Pelagic biome

text: metagenome

text: metagenome


characteristic: External Id

text: University of Trento

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

tag: Namespace:University of Trento

text: Italy

characteristic: geographic location (country and/or sea)

text: human gut metagenome

text: human gut metagenome

characteristic: title


characteristic: ENA-CHECKLIST

text: public

tag: Namespace:Bielefeld University, Medical School OWL  & Center for Biotechnology (CeBiTec)

text: 8 to 12

unit: week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: not collected

text: golden hamster

characteristic: common name

text: Protocols: At 21 days post infection, animals were sacrificed; subsequently, the lungs were harvested. Male and female golden hamsters (8-12 weeks old) were purchased from Janvier. All animals were kept under standard housing conditions (21 ± 2 °C, 40–50% humidity, food and water ad libitum) with a 12:12 light–dark cycle at the Leibniz Institute of Virology in Hamburg, Germany. As control, hamsters were treated by oral gavage with DMSO as vehicle. RNAprotect-fixed tissues were homogenized in 1 ml TRIZOL (Invitrogen) with 5 sterile, stainless steel beads (Ø 2 mm, Retch) at 30 Hz for 10 min in the mixer mill MM400 (Retsch). Cells were lysed in 500 µl or 1 ml TRIZOL (Invitrogen) per well, according to the well size, incubated for 5 min at RT. The cell lysate was resuspended and transferred into a fresh tube. 125 µl or 250 μl chloroform were added respectively to each sample, vortexed for 30 sec and subsequently centrifuged for 15 min at 13,000 g. Total RNA was isolated from homogenized tissues and the aqueous phase of the lysed cells using the innuPREP RNA Mini Kit 2.0 (Analytik Jena) according to the manufacturer’s instructions with an additional on column DNase I treatment using the RNase-free DNase Set (QIAGEN). Total RNA from formalin-fixed, paraffin-embedded human lung tissue sections was purified using the RNeasy@ FFPE Kit (Qiagen) according to the manufacturer’s instructions. The RNA was eluted in RNase-free water and mixed with 1 U μl-1 RiboLock RNase inhibitor (Thermo Fisher Scientific). Preparation of stranded cDNA libraries was performed with Illumina Stranded mRNA Prep, Ligation kit, starting with 1000 ng total RNA according to the manufacturer’s instructions.

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: not collected

characteristic: individual

characteristic: infect

text: not applicable

characteristic: isolate

text: Mesocricetus auratus

characteristic: organism

text: Mesocricetus auratus

text: lung

characteristic: organism part

text: I

characteristic: replicate

text: Mesocricetus auratus

text: female

characteristic: sex


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Bielefeld University, Medical School OWL  & Center for Biotechnology (CeBiTec)

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 8 to 12

text: ArrayExpress

text: not collected

characteristic: collection date

text: golden hamster

characteristic: description

text: adult

text: wild type genotype

text: not collected

text: not applicable

text: Mesocricetus auratus

text: Mesocricetus auratus

text: lung

text: II

text: Mesocricetus auratus

text: female


characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

text: 8 to 12

characteristic: age

text: ArrayExpress

characteristic: broker name

text: not collected

text: golden hamster

characteristic: common name

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

text: Mesocricetus auratus

text: Mesocricetus auratus

text: lung

text: III

text: Mesocricetus auratus

text: female

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: 8 to 12

text: ArrayExpress

text: not collected

text: golden hamster

text: adult

text: wild type genotype

text: not collected

characteristic: infect

text: not applicable

characteristic: isolate

text: Mesocricetus auratus

characteristic: organism

text: Mesocricetus auratus

text: lung

characteristic: organism part

text: IV

characteristic: replicate

text: Mesocricetus auratus

text: female

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 8 to 12

characteristic: age

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: golden hamster

characteristic: description

text: adult

text: wild type genotype

text: not collected

text: not applicable

text: Mesocricetus auratus

text: Mesocricetus auratus

text: lung

text: V

text: Mesocricetus auratus

text: female


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: 8 to 12

text: ArrayExpress

text: not collected

text: golden hamster

characteristic: common name

text: Protocols: At 21 days post infection, animals were sacrificed; subsequently, the lungs were harvested. Male and female golden hamsters (8-12 weeks old) were purchased from Janvier. All animals were kept under standard housing conditions (21 ± 2 °C, 40–50% humidity, food and water ad libitum) with a 12:12 light–dark cycle at the Leibniz Institute of Virology in Hamburg, Germany. For SARS-CoV-2 infection, hamsters were anaesthetized with 150 mg/kg ketamine and 10 mg kg-1 xylazine by intraperitoneal injection. The animals were intranasally inoculated with 105 plaque forming units (p.f.u.) As control, hamsters were treated by oral gavage with DMSO as vehicle. RNAprotect-fixed tissues were homogenized in 1 ml TRIZOL (Invitrogen) with 5 sterile, stainless steel beads (Ø 2 mm, Retch) at 30 Hz for 10 min in the mixer mill MM400 (Retsch). Cells were lysed in 500 µl or 1 ml TRIZOL (Invitrogen) per well, according to the well size, incubated for 5 min at RT. The cell lysate was resuspended and transferred into a fresh tube. 125 µl or 250 μl chloroform were added respectively to each sample, vortexed for 30 sec and subsequently centrifuged for 15 min at 13,000 g. Total RNA was isolated from homogenized tissues and the aqueous phase of the lysed cells using the innuPREP RNA Mini Kit 2.0 (Analytik Jena) according to the manufacturer’s instructions with an additional on column DNase I treatment using the RNase-free DNase Set (QIAGEN). Total RNA from formalin-fixed, paraffin-embedded human lung tissue sections was purified using the RNeasy@ FFPE Kit (Qiagen) according to the manufacturer’s instructions. The RNA was eluted in RNase-free water and mixed with 1 U μl-1 RiboLock RNase inhibitor (Thermo Fisher Scientific). Preparation of stranded cDNA libraries was performed with Illumina Stranded mRNA Prep, Ligation kit, starting with 1000 ng total RNA according to the manufacturer’s instructions.

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: Severe acute respiratory syndrome coronavirus 2

text: not applicable

text: Mesocricetus auratus

text: Mesocricetus auratus

text: lung

text: I

text: Mesocricetus auratus

text: female

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: 8 to 12

text: ArrayExpress

text: not collected

text: golden hamster

text: adult

text: wild type genotype

text: not collected

characteristic: infect

text: not applicable

characteristic: isolate

text: Mesocricetus auratus

characteristic: organism

text: Mesocricetus auratus

text: lung

characteristic: organism part

text: II

characteristic: replicate

text: Mesocricetus auratus

text: female

characteristic: sex


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: 8 to 12

characteristic: age

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: golden hamster

characteristic: description

text: adult

text: wild type genotype

text: not collected

text: not applicable

text: Mesocricetus auratus

text: Mesocricetus auratus

text: lung

text: III

text: Mesocricetus auratus

text: female


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: 8 to 12

text: ArrayExpress

text: not collected

text: golden hamster

characteristic: common name

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

text: Mesocricetus auratus

text: Mesocricetus auratus

text: lung

text: IV

text: Mesocricetus auratus

text: female

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: 8 to 12

text: ArrayExpress

text: not collected

text: golden hamster

text: adult

text: wild type genotype

text: not collected

characteristic: infect

text: not applicable

characteristic: isolate

text: Mesocricetus auratus

characteristic: organism

text: Mesocricetus auratus

text: lung

characteristic: organism part

text: V

characteristic: replicate

text: Mesocricetus auratus

text: female

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13100:53.1_Female_Cov2_Letrozole_1

characteristic: Submitter Id

text: 8 to 12

characteristic: age

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: golden hamster

text: Protocols: At 21 days post infection, animals were sacrificed; subsequently, the lungs were harvested. Male and female golden hamsters (8-12 weeks old) were purchased from Janvier. All animals were kept under standard housing conditions (21 ± 2 °C, 40–50% humidity, food and water ad libitum) with a 12:12 light–dark cycle at the Leibniz Institute of Virology in Hamburg, Germany. For SARS-CoV-2 infection, hamsters were anaesthetized with 150 mg/kg ketamine and 10 mg kg-1 xylazine by intraperitoneal injection. The animals were intranasally inoculated with 105 plaque forming units (p.f.u.) Letrozole dosing was calculated for male and female hamsters by taking sex-specific half times into consideration as reported before (45-47, 58, 59). For letrozole treatment, male and female hamsters received a final dose of 0.36 mg kg-1 d-1 or 0.18 mg kg-1 d-1 letrozole, respectively, (dissolved in DMSO, Sigma-Aldrich, L6545) diluted in sterile saline (Braun), respectively, by oral gavage (stainless-steel gavaging needles) at 3 h and day 1 to 3 post infection (p.i.). RNAprotect-fixed tissues were homogenized in 1 ml TRIZOL (Invitrogen) with 5 sterile, stainless steel beads (Ø 2 mm, Retch) at 30 Hz for 10 min in the mixer mill MM400 (Retsch). Cells were lysed in 500 µl or 1 ml TRIZOL (Invitrogen) per well, according to the well size, incubated for 5 min at RT. The cell lysate was resuspended and transferred into a fresh tube. 125 µl or 250 μl chloroform were added respectively to each sample, vortexed for 30 sec and subsequently centrifuged for 15 min at 13,000 g. Total RNA was isolated from homogenized tissues and the aqueous phase of the lysed cells using the innuPREP RNA Mini Kit 2.0 (Analytik Jena) according to the manufacturer’s instructions with an additional on column DNase I treatment using the RNase-free DNase Set (QIAGEN). Total RNA from formalin-fixed, paraffin-embedded human lung tissue sections was purified using the RNeasy@ FFPE Kit (Qiagen) according to the manufacturer’s instructions. The RNA was eluted in RNase-free water and mixed with 1 U μl-1 RiboLock RNase inhibitor (Thermo Fisher Scientific). Preparation of stranded cDNA libraries was performed with Illumina Stranded mRNA Prep, Ligation kit, starting with 1000 ng total RNA according to the manufacturer’s instructions.

characteristic: description

text: adult

text: wild type genotype

text: not collected

text: not applicable

text: Mesocricetus auratus

text: Mesocricetus auratus

text: lung

text: I

text: Mesocricetus auratus

text: female


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-13100:53.2_Female_Cov2_Letrozole_2

text: 8 to 12

text: ArrayExpress

text: not collected

text: golden hamster

characteristic: common name

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

text: Mesocricetus auratus

text: Mesocricetus auratus

text: lung

text: II

text: Mesocricetus auratus

text: female

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-13100:53.3_Female_Cov2_Letrozole_3

text: 8 to 12

text: ArrayExpress

text: not collected

text: golden hamster

text: adult

text: wild type genotype

text: not collected

text: SARS-CoV-2 (10^5 pfu)

characteristic: infect

text: not applicable

characteristic: isolate

text: Mesocricetus auratus

characteristic: organism

text: Mesocricetus auratus

text: lung

characteristic: organism part

text: III

characteristic: replicate

text: Mesocricetus auratus

text: female

characteristic: sex


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: SRA accession

text: E-MTAB-13100:53.4_Female_Cov2_Letrozole_4

characteristic: Submitter Id

text: 8 to 12

characteristic: age

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: golden hamster

characteristic: description

text: adult

text: wild type genotype

text: not collected

text: not applicable

text: Mesocricetus auratus

text: Mesocricetus auratus

text: lung

text: IV

text: Mesocricetus auratus

text: female


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-13100:54.1_Female_Cov2_Letrozole_5

text: 8 to 12

text: ArrayExpress

text: not collected

text: golden hamster

characteristic: common name

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

text: Mesocricetus auratus

text: Mesocricetus auratus

text: lung

text: V

text: Mesocricetus auratus

text: female

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: 8 to 12

text: ArrayExpress

text: not collected

text: golden hamster

text: adult

text: wild type genotype

text: not collected

characteristic: infect

text: not applicable

characteristic: isolate

text: Mesocricetus auratus

characteristic: organism

text: Mesocricetus auratus

text: lung

characteristic: organism part

text: I

characteristic: replicate

text: Mesocricetus auratus

text: male

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 8 to 12

characteristic: age

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: golden hamster

characteristic: description

text: adult

text: wild type genotype

text: not collected

text: not applicable

text: Mesocricetus auratus

text: Mesocricetus auratus

text: lung

text: II

text: Mesocricetus auratus

text: male


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: 8 to 12

text: ArrayExpress

text: not collected

text: golden hamster

characteristic: common name

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

text: Mesocricetus auratus

text: Mesocricetus auratus

text: lung

text: III

text: Mesocricetus auratus

text: male

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: 8 to 12

text: ArrayExpress

text: not collected

text: golden hamster

text: adult

text: wild type genotype

text: not collected

characteristic: infect

text: not applicable

characteristic: isolate

text: Mesocricetus auratus

characteristic: organism

text: Mesocricetus auratus

text: lung

characteristic: organism part

text: IV

characteristic: replicate

text: Mesocricetus auratus

text: male

characteristic: sex


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: 8 to 12

characteristic: age

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: golden hamster

characteristic: description

text: adult

text: wild type genotype

text: not collected

text: not applicable

text: Mesocricetus auratus

text: Mesocricetus auratus

text: lung

text: V

text: Mesocricetus auratus

text: male


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: 8 to 12

text: ArrayExpress

text: not collected

text: golden hamster

characteristic: common name

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

text: Mesocricetus auratus

text: Mesocricetus auratus

text: lung

text: I

text: Mesocricetus auratus

text: male

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: 8 to 12

text: ArrayExpress

text: not collected

text: golden hamster

text: adult

text: wild type genotype

text: not collected

characteristic: infect

text: not applicable

characteristic: isolate

text: Mesocricetus auratus

characteristic: organism

text: Mesocricetus auratus

text: lung

characteristic: organism part

text: II

characteristic: replicate

text: Mesocricetus auratus

text: male

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 8 to 12

characteristic: age

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: golden hamster

characteristic: description

text: adult

text: wild type genotype

text: not collected

text: not applicable

text: Mesocricetus auratus

text: Mesocricetus auratus

text: lung

text: III

text: Mesocricetus auratus

text: male


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: 8 to 12

text: ArrayExpress

text: not collected

text: golden hamster

characteristic: common name

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

text: Mesocricetus auratus

text: Mesocricetus auratus

text: lung

text: IV

text: Mesocricetus auratus

text: male

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: 8 to 12

text: ArrayExpress

text: not collected

text: golden hamster

text: adult

text: wild type genotype

text: not collected

characteristic: infect

text: not applicable

characteristic: isolate

text: Mesocricetus auratus

characteristic: organism

text: Mesocricetus auratus

text: lung

characteristic: organism part

text: V

characteristic: replicate

text: Mesocricetus auratus

text: male

characteristic: sex


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: 8 to 12

characteristic: age

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: golden hamster

characteristic: description

text: adult

text: wild type genotype

text: not collected

text: not applicable

text: Mesocricetus auratus

text: Mesocricetus auratus

text: lung

text: I

text: Mesocricetus auratus

text: male


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: 8 to 12

text: ArrayExpress

text: not collected

text: golden hamster

characteristic: common name

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

text: Mesocricetus auratus

text: Mesocricetus auratus

text: lung

text: II

text: Mesocricetus auratus

text: male

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: 8 to 12

text: ArrayExpress

text: not collected

text: golden hamster

text: adult

text: wild type genotype

text: not collected

characteristic: infect

text: not applicable

characteristic: isolate

text: Mesocricetus auratus

characteristic: organism

text: Mesocricetus auratus

text: lung

characteristic: organism part

text: III

characteristic: replicate

text: Mesocricetus auratus

text: male

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 8 to 12

characteristic: age

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: golden hamster

characteristic: description

text: adult

text: wild type genotype

text: not collected

text: not applicable

text: Mesocricetus auratus

text: Mesocricetus auratus

text: lung

text: IV

text: Mesocricetus auratus

text: male


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: 8 to 12

text: ArrayExpress

text: not collected

text: golden hamster

characteristic: common name

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

text: Mesocricetus auratus

text: Mesocricetus auratus

text: lung

text: V

text: Mesocricetus auratus

text: male

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

tag: Namespace:FEDERAL INSTITUTE FOR RISK ASSESSMENT (BFR)

text: not provided

text: DNA sample for all participants

text: not provided

text: ATCC 13932

characteristic: isolate

text: human

text: Listeria monocytogenes

characteristic: organism

text: Listeria monocytogenes


text: FEDERAL INSTITUTE FOR RISK ASSESSMENT (BFR)

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: not provided

characteristic: collection date

text: DNA sample for all participants

characteristic: description

text: not provided

text: ATCC 13076

text: not provided

text: Salmonella enterica subsp. enterica

text: Salmonella enterica subsp. enterica


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: Ring trial sample of participant LC01_LS

text: Germany

text: food

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

characteristic: geographic location (country and/or sea)

text: environment

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: feed

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: animal

text: Salmonella enterica subsp. enterica

text: Salmonella enterica subsp. enterica


text: public

text: Germany

text: animal

text: Salmonella enterica subsp. arizonae

text: Salmonella enterica subsp. arizonae


text: public

text: Germany

text: food

text: Salmonella enterica subsp. enterica

text: Salmonella enterica subsp. enterica


text: public

text: Ring trial sample of participant LC02_LS

text: Germany

text: food

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: environment

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: feed

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: animal

text: Salmonella enterica subsp. enterica

text: Salmonella enterica subsp. enterica


text: public

text: Germany

text: animal

text: Salmonella enterica subsp. arizonae

text: Salmonella enterica subsp. arizonae


text: public

text: Germany

text: food

text: Salmonella enterica subsp. enterica

text: Salmonella enterica subsp. enterica


text: public

text: Ring trial sample of participant LC03_LS

text: Germany

text: food

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: environment

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: feed

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: animal

text: Salmonella enterica subsp. enterica

text: Salmonella enterica subsp. enterica


text: public

text: Germany

text: animal

text: Salmonella enterica subsp. arizonae

text: Salmonella enterica subsp. arizonae


text: public

text: Germany

text: food

text: Salmonella enterica subsp. enterica

text: Salmonella enterica subsp. enterica


text: public

text: Ring trial sample of participant LC04_LS

text: Germany

text: food

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: environment

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: feed

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: animal

text: Salmonella enterica subsp. enterica

text: Salmonella enterica subsp. enterica


text: public

text: Germany

text: animal

text: Salmonella enterica subsp. arizonae

text: Salmonella enterica subsp. arizonae


text: public

text: Germany

text: food

text: Salmonella enterica subsp. enterica

text: Salmonella enterica subsp. enterica


text: public

text: Ring trial sample of participant LC04_S5_LS

text: Germany

text: food

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: environment

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: feed

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: animal

text: Salmonella enterica subsp. enterica

text: Salmonella enterica subsp. enterica


text: public

text: Germany

text: animal

text: Salmonella enterica subsp. arizonae

text: Salmonella enterica subsp. arizonae


text: public

text: Germany

text: food

text: Salmonella enterica subsp. enterica

text: Salmonella enterica subsp. enterica


text: public

text: Ring trial sample of participant LC05_LS

text: Germany

text: food

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: environment

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: feed

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: animal

text: Salmonella enterica subsp. enterica

text: Salmonella enterica subsp. enterica


text: public

text: Germany

text: animal

text: Salmonella enterica subsp. arizonae

text: Salmonella enterica subsp. arizonae


text: public

text: Germany

text: food

text: Salmonella enterica subsp. enterica

text: Salmonella enterica subsp. enterica


text: public

text: Ring trial sample of participant LC06_LS

text: Germany

text: food

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: environment

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: feed

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: animal

text: Salmonella enterica subsp. enterica

text: Salmonella enterica subsp. enterica


text: public

text: Germany

text: animal

text: Salmonella enterica subsp. arizonae

text: Salmonella enterica subsp. arizonae


text: public

text: Germany

text: food

text: Salmonella enterica subsp. enterica

text: Salmonella enterica subsp. enterica


text: public

text: Ring trial sample of participant LC07_LS

text: Germany

text: food

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: environment

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: feed

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: animal

text: Salmonella enterica subsp. enterica

text: Salmonella enterica subsp. enterica


text: public

text: Germany

text: animal

text: Salmonella enterica subsp. arizonae

text: Salmonella enterica subsp. arizonae


text: public

text: Germany

text: food

text: Salmonella enterica subsp. enterica

text: Salmonella enterica subsp. enterica


text: public

text: Ring trial sample of participant LC08_LS

text: Germany

text: food

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: environment

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: feed

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: animal

text: Salmonella enterica subsp. enterica

text: Salmonella enterica subsp. enterica


text: public

text: Germany

text: animal

text: Salmonella enterica subsp. arizonae

text: Salmonella enterica subsp. arizonae


text: public

text: Germany

text: food

text: Salmonella enterica subsp. enterica

text: Salmonella enterica subsp. enterica


text: public

text: Ring trial sample of participant LC09_LS

text: Germany

text: food

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: environment

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: feed

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: animal

text: Salmonella enterica subsp. enterica

text: Salmonella enterica subsp. enterica


text: public

text: Germany

text: animal

text: Salmonella enterica subsp. arizonae

text: Salmonella enterica subsp. arizonae


text: public

text: Germany

text: food

text: Salmonella enterica subsp. enterica

text: Salmonella enterica subsp. enterica


text: public

text: Ring trial sample of participant LC10_LS

text: Germany

text: food

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: environment

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: feed

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: animal

text: Salmonella enterica subsp. enterica

text: Salmonella enterica subsp. enterica


text: public

text: Germany

text: animal

text: Salmonella enterica subsp. arizonae

text: Salmonella enterica subsp. arizonae


text: public

text: Germany

text: food

text: Salmonella enterica subsp. enterica

text: Salmonella enterica subsp. enterica


text: public

text: Ring trial sample of participant LC11_LS

text: Germany

text: food

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: environment

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: feed

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: animal

text: Salmonella enterica subsp. enterica

text: Salmonella enterica subsp. enterica


text: public

text: Germany

text: animal

text: Salmonella enterica subsp. arizonae

text: Salmonella enterica subsp. arizonae


text: public

text: Germany

text: food

text: Salmonella enterica subsp. enterica

text: Salmonella enterica subsp. enterica


text: public

text: Ring trial sample of participant LC12_LS

text: Germany

text: food

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: environment

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: feed

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: animal

text: Salmonella enterica subsp. enterica

text: Salmonella enterica subsp. enterica


text: public

text: Germany

text: animal

text: Salmonella enterica subsp. arizonae

text: Salmonella enterica subsp. arizonae


text: public

text: Germany

text: food

text: Salmonella enterica subsp. enterica

text: Salmonella enterica subsp. enterica


text: public

text: Ring trial sample of participant LC13_S5_LS

text: Germany

text: food

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: environment

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: feed

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: animal

text: Salmonella enterica subsp. enterica

text: Salmonella enterica subsp. enterica


text: public

text: Germany

text: animal

text: Salmonella enterica subsp. arizonae

text: Salmonella enterica subsp. arizonae


text: public

text: Germany

text: food

text: Salmonella enterica subsp. enterica

text: Salmonella enterica subsp. enterica


text: public

text: Ring trial sample of participant LC14_S5_LS

text: Germany

text: food

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: environment

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: feed

text: Listeria monocytogenes

text: Listeria monocytogenes


text: public

text: Germany

text: animal

text: Salmonella enterica subsp. enterica

text: Salmonella enterica subsp. enterica


text: public

text: Germany

text: animal

text: Salmonella enterica subsp. arizonae

text: Salmonella enterica subsp. arizonae


text: public

text: Germany

text: food

text: Salmonella enterica subsp. enterica

text: Salmonella enterica subsp. enterica


text: public

text: DNA sample for all participants

text: not provided

text: WDCM 00005, ATCC 33291, BfR-CA-17998

text: human

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: not provided

text: DNA sample for all participants

text: not provided

text: BfR-EC-17177, EURL-strain ED56

text: animal

text: Escherichia coli

text: Escherichia coli


text: public

text: Ring trial sample of participant LC01_CE

text: Germany

text: BfR-CA-17135, 22-CA00136

text: animal

text: Campylobacter coli

text: Campylobacter coli


text: FEDERAL INSTITUTE FOR RISK ASSESSMENT (BFR)

text: public

text: Germany

text: BfR-CA-17135, 22-CA00137

text: animal

text: Campylobacter coli

text: Campylobacter coli


text: public

text: Germany

text: BfR-CA-15954, 22-CA00138

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-16317, 22-CA00139

text: human

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-15146, 22-CA00140

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-15160, 22-CA00141

text: human

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-17201, 22-CA00142

text: food

text: Campylobacter lari

text: Campylobacter lari


text: public

text: Germany

text: BfR-CA-14973, 22-CA00143

text: food

text: Campylobacter coli

text: Campylobacter coli


text: public

text: Germany

text: BfR-CA-13985, 22-CA00144

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-14430, 22-CA00145

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: food

text: Escherichia coli

text: Escherichia coli


text: public

text: Germany

text: animal

text: Escherichia coli

text: Escherichia coli


text: public

text: Ring trial sample of participant LC02_CE

text: Germany

text: BfR-CA-17135, 22-CA00136

text: animal

text: Campylobacter coli

text: Campylobacter coli


text: public

text: Germany

text: BfR-CA-17135, 22-CA00137

text: animal

text: Campylobacter coli

text: Campylobacter coli


text: public

text: Germany

text: BfR-CA-15954, 22-CA00138

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-16317, 22-CA00139

text: human

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-15146, 22-CA00140

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-15160, 22-CA00141

text: human

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-17201, 22-CA00142

text: food

text: Campylobacter lari

text: Campylobacter lari


text: public

text: Germany

text: BfR-CA-14973, 22-CA00143

text: food

text: Campylobacter coli

text: Campylobacter coli


text: public

text: Germany

text: BfR-CA-13985, 22-CA00144

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-14430, 22-CA00145

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: food

text: Escherichia coli

text: Escherichia coli


text: public

text: Germany

text: animal

text: Escherichia coli

text: Escherichia coli


text: public

text: Ring trial sample of participant LC03_CE

text: Germany

text: BfR-CA-17135, 22-CA00136

text: animal

text: Campylobacter coli

text: Campylobacter coli


text: public

text: Germany

text: BfR-CA-17135, 22-CA00137

text: animal

text: Campylobacter coli

text: Campylobacter coli


text: public

text: Germany

text: BfR-CA-15954, 22-CA00138

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-16317, 22-CA00139

text: human

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-15146, 22-CA00140

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-15160, 22-CA00141

text: human

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-17201, 22-CA00142

text: food

text: Campylobacter lari

text: Campylobacter lari


text: public

text: Germany

text: BfR-CA-14973, 22-CA00143

text: food

text: Campylobacter coli

text: Campylobacter coli


text: public

text: Germany

text: BfR-CA-13985, 22-CA00144

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-14430, 22-CA00145

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: food

text: Escherichia coli

text: Escherichia coli


text: public

text: Germany

text: animal

text: Escherichia coli

text: Escherichia coli


text: public

text: Ring trial sample of participant LC04_CE

text: Germany

text: BfR-CA-17135, 22-CA00136

text: animal

text: Campylobacter coli

text: Campylobacter coli


text: public

text: Germany

text: BfR-CA-17135, 22-CA00137

text: animal

text: Campylobacter coli

text: Campylobacter coli


text: public

text: Germany

text: BfR-CA-15954, 22-CA00138

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-16317, 22-CA00139

text: human

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-15146, 22-CA00140

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-15160, 22-CA00141

text: human

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-17201, 22-CA00142

text: food

text: Campylobacter lari

text: Campylobacter lari


text: public

text: Germany

text: BfR-CA-14973, 22-CA00143

text: food

text: Campylobacter coli

text: Campylobacter coli


text: public

text: Germany

text: BfR-CA-13985, 22-CA00144

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-14430, 22-CA00145

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: food

text: Escherichia coli

text: Escherichia coli


text: public

text: Germany

text: animal

text: Escherichia coli

text: Escherichia coli


text: public

text: Ring trial sample of participant LC05_CE

text: Germany

text: BfR-CA-17135, 22-CA00136

text: animal

text: Campylobacter coli

text: Campylobacter coli


text: public

text: Germany

text: BfR-CA-17135, 22-CA00137

text: animal

text: Campylobacter coli

text: Campylobacter coli


text: public

text: Germany

text: BfR-CA-15954, 22-CA00138

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-16317, 22-CA00139

text: human

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-15146, 22-CA00140

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-15160, 22-CA00141

text: human

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-17201, 22-CA00142

text: food

text: Campylobacter lari

text: Campylobacter lari


text: public

text: Germany

text: BfR-CA-14973, 22-CA00143

text: food

text: Campylobacter coli

text: Campylobacter coli


text: public

text: Germany

text: BfR-CA-13985, 22-CA00144

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-14430, 22-CA00145

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: food

text: Escherichia coli

text: Escherichia coli


text: public

text: Germany

text: animal

text: Escherichia coli

text: Escherichia coli


text: public

text: Ring trial sample of participant LC06_CE

text: Germany

text: BfR-CA-17135, 22-CA00136

text: animal

text: Campylobacter coli

text: Campylobacter coli


text: public

text: Germany

text: BfR-CA-17135, 22-CA00137

text: animal

text: Campylobacter coli

text: Campylobacter coli


text: public

text: Germany

text: BfR-CA-15954, 22-CA00138

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-16317, 22-CA00139

text: human

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-15146, 22-CA00140

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-15160, 22-CA00141

text: human

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-17201, 22-CA00142

text: food

text: Campylobacter lari

text: Campylobacter lari


text: public

text: Germany

text: BfR-CA-14973, 22-CA00143

text: food

text: Campylobacter coli

text: Campylobacter coli


text: public

text: Germany

text: BfR-CA-13985, 22-CA00144

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-14430, 22-CA00145

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: food

text: Escherichia coli

text: Escherichia coli


text: public

text: Germany

text: animal

text: Escherichia coli

text: Escherichia coli


text: public

text: Ring trial sample of participant LC07_CE

text: Germany

text: BfR-CA-17135, 22-CA00136

text: animal

text: Campylobacter coli

text: Campylobacter coli


text: public

text: Germany

text: BfR-CA-17135, 22-CA00137

text: animal

text: Campylobacter coli

text: Campylobacter coli


text: public

text: Germany

text: BfR-CA-15954, 22-CA00138

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-16317, 22-CA00139

text: human

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-15146, 22-CA00140

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-15160, 22-CA00141

text: human

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-17201, 22-CA00142

text: food

text: Campylobacter lari

text: Campylobacter lari


text: public

text: Germany

text: BfR-CA-14973, 22-CA00143

text: food

text: Campylobacter coli

text: Campylobacter coli


text: public

text: Germany

text: BfR-CA-13985, 22-CA00144

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-14430, 22-CA00145

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: food

text: Escherichia coli

text: Escherichia coli


text: public

text: Germany

text: animal

text: Escherichia coli

text: Escherichia coli


text: public

text: Ring trial sample of participant LC08_CE

text: Germany

text: BfR-CA-17135, 22-CA00136

text: animal

text: Campylobacter coli

text: Campylobacter coli


text: public

text: Germany

text: BfR-CA-17135, 22-CA00137

text: animal

text: Campylobacter coli

text: Campylobacter coli


text: public

text: Germany

text: BfR-CA-15954, 22-CA00138

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-16317, 22-CA00139

text: human

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-15146, 22-CA00140

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-15160, 22-CA00141

text: human

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-17201, 22-CA00142

text: food

text: Campylobacter lari

text: Campylobacter lari


text: public

text: Germany

text: BfR-CA-14973, 22-CA00143

text: food

text: Campylobacter coli

text: Campylobacter coli


text: public

text: Germany

text: BfR-CA-13985, 22-CA00144

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-14430, 22-CA00145

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: food

text: Escherichia coli

text: Escherichia coli


text: public

text: Germany

text: animal

text: Escherichia coli

text: Escherichia coli


text: public

text: Ring trial sample of participant LC09_CE

text: Germany

text: BfR-CA-17135, 22-CA00136

text: animal

text: Campylobacter coli

text: Campylobacter coli


text: public

text: Germany

text: BfR-CA-17135, 22-CA00137

text: animal

text: Campylobacter coli

text: Campylobacter coli


text: public

text: Germany

text: BfR-CA-15954, 22-CA00138

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-16317, 22-CA00139

text: human

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-15146, 22-CA00140

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-15160, 22-CA00141

text: human

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-17201, 22-CA00142

text: food

text: Campylobacter lari

text: Campylobacter lari


text: public

text: Germany

text: BfR-CA-14973, 22-CA00143

text: food

text: Campylobacter coli

text: Campylobacter coli


text: public

text: Germany

text: BfR-CA-13985, 22-CA00144

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-14430, 22-CA00145

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: food

text: Escherichia coli

text: Escherichia coli


text: public

text: Germany

text: animal

text: Escherichia coli

text: Escherichia coli


text: public

text: Ring trial sample of participant LC10_CE

text: Germany

text: BfR-CA-17135, 22-CA00136

text: animal

text: Campylobacter coli

text: Campylobacter coli


text: public

text: Germany

text: BfR-CA-17135, 22-CA00137

text: animal

text: Campylobacter coli

text: Campylobacter coli


text: public

text: Germany

text: BfR-CA-15954, 22-CA00138

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-16317, 22-CA00139

text: human

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-15146, 22-CA00140

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-15160, 22-CA00141

text: human

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-17201, 22-CA00142

text: food

text: Campylobacter lari

text: Campylobacter lari


text: public

text: Germany

text: BfR-CA-14973, 22-CA00143

text: food

text: Campylobacter coli

text: Campylobacter coli


text: public

text: Germany

text: BfR-CA-13985, 22-CA00144

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-14430, 22-CA00145

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: food

text: Escherichia coli

text: Escherichia coli


text: public

text: Germany

text: animal

text: Escherichia coli

text: Escherichia coli


text: public

text: Ring trial sample of participant LC11_CE

text: Germany

text: BfR-CA-17135, 22-CA00136

text: animal

text: Campylobacter coli

text: Campylobacter coli


text: public

text: Germany

text: BfR-CA-17135, 22-CA00137

text: animal

text: Campylobacter coli

text: Campylobacter coli


text: public

text: Germany

text: BfR-CA-15954, 22-CA00138

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-16317, 22-CA00139

text: human

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-15146, 22-CA00140

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-15160, 22-CA00141

text: human

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-17201, 22-CA00142

text: food

text: Campylobacter lari

text: Campylobacter lari


text: public

text: Germany

text: BfR-CA-14973, 22-CA00143

text: food

text: Campylobacter coli

text: Campylobacter coli


text: public

text: Germany

text: BfR-CA-13985, 22-CA00144

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: BfR-CA-14430, 22-CA00145

text: food

text: Campylobacter jejuni

text: Campylobacter jejuni


text: public

text: Germany

text: food

text: Escherichia coli

text: Escherichia coli


text: public

text: Germany

text: animal

text: Escherichia coli

text: Escherichia coli


text: public

text: Ring trial sample of participant LC12_CE

text: Germany

text: BfR-CA-17135, 22-CA00136

text: animal

text: Campylobacter coli

text: Campylobacter coli