characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF MANCHESTER

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: four-striped grass mouse

characteristic: description

text: adult

text: normal

text: wild type genotype

text: 12 hour light-dark cycle

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text: not applicable

text: Rhabdomys pumilio

text: Rhabdomys pumilio

text: lung

text: Rhabdomys pumilio

text: male

characteristic: sex


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

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text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

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tag: Namespace:UNIVERSITY OF MANCHESTER

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text: ArrayExpress

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text: Protocols: Animals were sedated with isoflurane and immediately culled by cervical dislocation at ZT2 or ZT14. Retina, lung and liver tissues was dissected and snap frozen immediately. For SCN samples, punches were collected from 1 mm thick brain slices containing a sample corer. All samples were stored at -80°C until RNA extraction. For retina, lung and liver samples total RNA was extracted from < 5 mg frozen tissue using the Qiagen RNeasy Mini kit (Qiagen 74104). For SCN, total RNA was extracted from snap-frozen brain punches using the Qiagen RNeasy Micro kit (Qiagen 74004). All samples were quantified using a 4200 TapeStation (Agilent Technologies). For retina, lung and liver samples, cDNA libraries were generated using the Illumina Stranded mRNA Prep Ligation kit (Illumina, Inc. 20040532). For SCN samples, cDNA libraries were prepared using the SMARTer Stranded Total RNA-Seq Kit V3 - Pico Input Mammalian (Takara Bio 634485).

text: adult

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text: UNIVERSITY OF MANCHESTER

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text: four-striped grass mouse

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text: UNIVERSITY OF MANCHESTER

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text: Bacterial community in feces of sample ANMA0612

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characteristic: ENA-LAST-UPDATE

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text: Bacterial community in feces of sample ANRO1212

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text: Bacterial community in feces of sample CIGI1807

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text: Bacterial community in feces of sample DADO0205

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text: public

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text: Bacterial community in feces of sample DOOL2004

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characteristic: organism

text: human gut metagenome

text: Miseq PE 2x300


characteristic: ENA-LAST-UPDATE

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text: public

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text: Bacterial community in feces of sample ELMA2603

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text: public

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text: Bacterial community in feces of sample GISI1405

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text: public

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text: Bacterial community in feces of sample LAAL1207

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text: human gut metagenome

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characteristic: ENA-CHECKLIST

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text: public

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text: metagenomes

text: Bacterial community in feces of sample LAFA3004

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text: fecal material

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text: Italy

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characteristic: title


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text: public

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text: Bacterial community in feces of sample LOFR2903

text: digestive tract environment

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text: fecal material

text: Italy

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text: healthy

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text: public

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text: Bacterial community in feces of sample LUGI3006

text: digestive tract environment

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text: fecal material

text: Italy

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text: human gut metagenome

text: Miseq PE 2x300


characteristic: ENA-LAST-UPDATE

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text: public

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text: metagenomes

text: Bacterial community in feces of sample LUMA2811

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text: fecal material

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text: Italy

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text: human gut metagenome

text: Miseq PE 2x300

characteristic: title


characteristic: ENA-CHECKLIST

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text: public

text: metagenomes

text: Bacterial community in feces of sample MAGI1111

text: digestive tract environment

text: intestine environment

text: fecal material

text: Italy

characteristic: geographic location (latitude)

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text: healthy

characteristic: host disease status

text: human-gut

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text: metagenome

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text: Miseq PE 2x300

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characteristic: ENA-LAST-UPDATE

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text: public

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characteristic: common name

text: Bacterial community in feces of sample MAGI2408

text: digestive tract environment

text: intestine environment

text: fecal material

text: Italy

text: healthy

text: human-gut

text: metagenome

text: human gut metagenome

characteristic: organism

text: Fecal microbiota in eating disorder patients

text: human gut metagenome

text: Miseq PE 2x300


characteristic: ENA-CHECKLIST

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text: Institute of Agricultural Biology and Biotechnology - National Research Council (CNR)

text: public

characteristic: Submitter Id

text: metagenomes

text: Bacterial community in feces of sample MAMA1910

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text: intestine environment

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text: fecal material

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text: Italy

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text: Miseq PE 2x300

characteristic: title


characteristic: ENA-LAST-UPDATE

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text: public

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text: Bacterial community in feces of sample NAGI2712

text: digestive tract environment

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text: Italy

characteristic: geographic location (latitude)

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text: healthy

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text: public

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text: Bacterial community in feces of sample RORE3003

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text: Italy

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characteristic: organism

text: human gut metagenome

text: Miseq PE 2x300


characteristic: ENA-LAST-UPDATE

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text: public

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text: metagenomes

text: Bacterial community in feces of sample SAGI2007

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text: fecal material

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text: Italy

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text: Miseq PE 2x300

characteristic: title


characteristic: ENA-CHECKLIST

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text: public

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text: Bacterial community in feces of sample SIDA2611

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text: public

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text: Bacterial community in feces of sample SIEN0101

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characteristic: organism

text: human gut metagenome

text: Miseq PE 2x300


characteristic: ENA-CHECKLIST

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text: public

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text: metagenomes

text: Bacterial community in feces of sample ED10

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characteristic: ENA-LAST-UPDATE

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text: Bacterial community in feces of sample ED13

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text: Bacterial community in feces of sample ED14

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text: public

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text: Bacterial community in feces of sample ED15

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characteristic: organism

text: human gut metagenome

text: Miseq PE 2x300


characteristic: ENA-CHECKLIST

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text: public

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text: metagenomes

text: Bacterial community in feces of sample ED16

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text: Bacterial community in feces of sample ED17

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text: Bacterial community in feces of sample ED18

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text: Miseq PE 2x300


characteristic: ENA-LAST-UPDATE

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text: public

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text: metagenomes

text: Bacterial community in feces of sample ED19

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text: Italy

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text: Fecal microbiota in eating disorder patients

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characteristic: title


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text: Institute of Agricultural Biology and Biotechnology - National Research Council (CNR)

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text: Bacterial community in feces of sample ED1

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text: healthy

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text: public

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text: metagenomes

characteristic: common name

text: Bacterial community in feces of sample ED20

text: digestive tract environment

text: intestine environment

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text: Italy

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text: human gut metagenome

characteristic: organism

text: human gut metagenome

text: Miseq PE 2x300


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: Submitter Id

text: metagenomes

text: Bacterial community in feces of sample ED21

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text: human-gut

text: metagenome

text: human gut metagenome

text: Fecal microbiota in eating disorder patients

text: human gut metagenome

text: Miseq PE 2x300

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UANTW

text: public

text: human saliva metagenome

text: human saliva metagenome

text: human saliva metagenome


text: RIVM

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Rijksinstituut voor Volksgezondheid en Milieu (RIVM), the Netherlands

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Netherlands Norovirus 2015-2019 surveillance

text: Netherlands

unit: years

characteristic: host age

text: human

characteristic: host common name

text: not provided

characteristic: host health state

text: homo sapiens

characteristic: host scientific name

text: female

characteristic: host sex

characteristic: host subject id

characteristic: isolate

text: human feces

characteristic: isolation source host-associated

text: human feces metagenome

characteristic: receipt date

text: active surveillance not initiated by an outbreak

characteristic: sample capture status

text: human feces metagenome


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: RIVM

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not provided

characteristic: description

text: Netherlands

characteristic: geographic location (country and/or sea)

text: human

text: not provided

text: homo sapiens

text: male

text: human feces

text: human feces metagenome

characteristic: organism

text: human feces metagenome

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: RIVM

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Netherlands

characteristic: host age

text: human

characteristic: host common name

text: not provided

characteristic: host health state

text: homo sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: human feces

text: human feces metagenome

text: human feces metagenome


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Royal Botanic Gardens Kew

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ROYAL BOTANIC GARDEN EDINBURGH

characteristic: barcoding center

text: COPO

characteristic: broker name

text: Maarten Christenhusz

text: Independent

unit: m

characteristic: elevation

text: United Kingdom

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: SURREY | KINGSTON UPON THAMES

characteristic: geographic location (region and locality)

text: Allotment

characteristic: habitat

text: Maarten Christenhusz

text: Independent

text: vegetative structure

characteristic: lifestage

text: Agrostis gigantea

text: WHOLE ORGANISM

characteristic: organism part

characteristic: original collection date

text: UNITED KINGDOM | SURREY | KINGSTON UPON THAMES

characteristic: original collection location

text: DTOL

characteristic: project name

text: Agrostis gigantea

text: HERMAPHRODITE MONOECIOUS

characteristic: sex

text: 4834ef3d-5fe4-4fb2-a1d9-c859b470f04e-DTOL-specimen

characteristic: tolid

type: derived from


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Royal Botanic Gardens Kew

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: ROYAL BOTANIC GARDEN EDINBURGH

text: COPO

text: Sahr Mian | Ilia Leitch | Keith Kirby | Andrew Leitch

text: Royal Botanice Gardens Kew | Queen Mary University of London | Oxford

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Oxford | Wytham Woods

text: Side of woodland road

text: Keith Kirby

text: Oxford | Wytham Woods

text: vegetative structure

text: Bromus benekenii

characteristic: organism

text: WHOLE ORGANISM

text: United Kingdom | Oxford | Wytham Woods

text: DTOL

text: Bromus benekenii

text: HERMAPHRODITE MONOECIOUS

text: 3fb92890-01b9-4ab1-889d-eac3b81bb2f4-DTOL-specimen

characteristic: title


text: EMBL

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12068:sn009_Mix_Pdu6dpf3N_7500c_Dmel_bcd4xG0_7500c_20201027_10x_SingleIndexA_F2

text: 2h30min to 3h30min

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: Protocols: 2.5h-to-3.5h-old embryos (developed in room temperature) were dechorionated and flash-frozen in liquid nitrogen for nuclei preparation. The evolved embryos are from population 2-6-1A, at the 20th generation. They were manually examined and smaller embryos were removed upon collection, to reduce noise and focus on relatively large embryos. A wild-type line (VK33) and the 4xbcd lab stock were treated in parallel, however without manual sorting. Nuclei isolation was performed following a standard protocol (10x Genomics Single Cell Protocols, with adaptations from Francisca Hervas-Sotomayor at Heidelberg University). The frozen embryos were squished with a pestle for 20 times in cold homogenisation buffer (HB) [250 mM sucrose, 25 mM KCl, 5 mM MgCl2, 10 mM Tris-HCl (pH 8), 0.1% Nonidet P40/IGEPAL, 1 uM DTT, 0.4 U/ul RNAse Inhibitor (New England Biolabs), 0.2 U/ul SUPERase In RNase Inhibitor (Invitrogen)]. The samples were then centrifuged at 100 g for 1 min to remove unlysed tissue, and the supernatant was centrifuged at 1,000 g for 5 min to pellet the nuclei. The pellet was washed once in HB, filtered twice with Flowmi Cell Strainers (Sigma) and resuspended in PBS. A subsample of the nuclei prep was DAPI-stained and examined under the microscope, to determine the density of nuclei. For each sample, 7,500 nuclei were used as the input for 10x library construction. The library was constructed following standard 10x Genomics protocol (Chromium Next GEM Single Cell 3_ Reagent Kits v3.1).

characteristic: description

text: Generation 0

characteristic: generation

text: transgenic

characteristic: genetic modification

text: bicoid-eGFP inserted at VK33

characteristic: genotype

text: egg laying

characteristic: initial time point

text: not applicable

characteristic: isolate

text: Drosophila melanogaster

text: Drosophila melanogaster

text: Drosophila melanogaster

text: mixed sex

characteristic: strain

characteristic: treatment


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMBL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: 2h30min to 3h30min

text: ArrayExpress

text: fruit fly

text: not applicable

text: wild type genotype

text: egg laying

text: not applicable

text: Drosophila melanogaster

text: Drosophila melanogaster

text: Drosophila melanogaster

text: mixed sex

characteristic: sex


text: EMBL

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 2h30min to 3h30min

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: Generation 20

text: transgenic

text: bicoid-eGFP inserted at VK33

text: egg laying

text: not applicable

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: Drosophila melanogaster

text: mixed sex

characteristic: title

text: EMS-mutagenesis at G0


name: E-MTAB-12033:HEK APPwt cells, mitochondrial RNA used for RT-PCR, biological replicate 1

characteristic: ENA-CHECKLIST

text: JOHANNES GUTENBERG UNIVERSITY MAINZ

text: public

tag: Namespace:JOHANNES GUTENBERG UNIVERSITY MAINZ

text: ArrayExpress

text: human

text: Protocols: HEK 293 cells were transfected with a DNA construct harboring the human APPwt gene, inserted downstream of a cytomegalovirus promoter using the FUGENE 6 technology (Roche Diagnostics). The stably expressing APPwt HEK 293 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, REF: 41965-039) supplemented with 10% heat-inactivated fetal calf serum (FCS) (Gibco, REF: 10270-106, Lot: 2156880), 50 Units/mL penicillin and 50 µg/mL streptomycin (Pen Strep, Gibco, REF: 15140-122), and 400 µg/mL G418 Solution (Roche, 4727894001) at 37 °C in a humidified incubator containing 5% CO2. Control cells (Ctl) (untransfected HEK 293) were cultured in the same medium, excluding G418. Thus, a mitochondria isolation was done in order to enrich the desired mitochondrial mRNA. Therefor HEK cells were washed with ice-cold PBS and seeded in large cell culture plates. After two days growing, medium was changed and on the third day the isolation of mitochondria was performed based on a Percollgradient. Cells were seeded two days in advance and washed with ice-cold PBS for synchronization. Cells were harvested at a confluency of 70-80% in 1 mL TRI Reagent®. Dissolution of the cells was supported by vortexing and incubation at room temperature for 5 min. Next 200 µL of chloroform (Sigma-Aldrich, C2432) was added and mixed thoroughly. After a second incubation step (10 min) at 21 °C, the cell suspension was centrifuged (16 000 x g, 4 °C, 15 min). The upper aqueous phase was taken and mixed in a 1:1 ratio with isopropanol (ROTH, 7343.1) and 1 µL glycogen (Thermo Scientific, R0551) was added, incubated at 21 °C (5 min) and centrifuged (16 000 x g, 4 °C, 10 min). The RNA pellet was washed twice with ice-cold 75% ethanol including centrifugation (16 000 x g, 4 °C, 5 min). RNA was reconstituted in 10-20 µL nuclease-free water (Invitrogen, AM9930). RNA concentrations were determined using UV-VIS spectrophotometer Nanodrop 2000 (Thermo Fisher Scientific, Waltham, USA). Total RNA and RNA from mitochondrial extracts were incubated with DNase I (Zymo Research, E1010) and 10x DNase digestion Buffer (Zymo Research, E1010) at 21 °C (15 min) to eliminate any existing DNA contamination and a second phenol-chloroform extraction was performed as described above. For reverse transcription 2 µg RNA, 0.5 mM dNTPs (Thermo Scientific, R0192), water and a target-specific RT primer (see Supplementary Table S1) (2.5 µM) were mixed and incubated for 5 min at 75 °C and afterwards 5 min on ice. Next 10 U/µL SuperScript-IV (Invitrogen, 18090050), dithiothreitol (DTT) and 5x RT Buffer and were added, mixed and incubated for 2 hours at 50 °C. In the end, enzyme was inactivated at 80 °C for 15 min. The RT Primer contained a target-specific sequence complementary to ND5 mRNA and a primer-binding site for indexing with the NEBNext® Multiplex Small RNA Library Prep Kit for Illumina® (New England Biolabs, #E7560S) primers afterwards during polymerase chain reaction (PCR). PCR was conducted with 0.04 U/µL Platinum SuperFi (Invitrogen, 12351010), 5x SuperFi buffer (Invitrogen, 12351010), 0.2 mM dNTPs, 5x GC Enhancer (Invitrogen, 12351010), 0.5 µM custom-designed forward primer (see Supplementary Table S1) and reverse primers of the NEBNext® Multiplex Small RNA Library Prep Kit for Illumina®. The custom p5 PCR primer contained flow cell binding sequence, sequencing primer site 1 and a target-specific sequence complementary to ND5 cDNA. The commercial i7 index primers introduced individual barcodes for each sample, contained the sequencing primer site 2 and the second flow cell binding sequence. PCR was started in Thermocycler peqSTAR (VWR Peqlab), including an initial denaturation step at 98 °C (2 min), 30 cycles at 98 °C (15 min), 63 °C (1 min) and 72 °C (2 min), and a final extension step at 72°C (5 min). PCR products were purified on a 10% denaturing polyacrylamide gel by size selection and the expected full-length product was excised. Finally, prepared libraries were quality controlled for adapter dimer formation on an Agilent 4200 TapeStation (Agilent, Santa Clara, United States).

text: embryo

characteristic: developmental stage

text: APPwt

characteristic: genotype

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: kidney

characteristic: organism part

text: APPwt expression

characteristic: phenotype

text: Homo sapiens


name: E-MTAB-12033:HEK APPwt cells, mitochondrial RNA used for RT-PCR, biological replicate 2

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: JOHANNES GUTENBERG UNIVERSITY MAINZ

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: human

text: embryo

text: APPwt

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: kidney

text: APPwt expression

text: Homo sapiens


name: E-MTAB-12033:HEK APPwt cells, Total RNA used for RT-PCR, biological replicate 1

text: JOHANNES GUTENBERG UNIVERSITY MAINZ

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: human

text: embryo

text: APPwt

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: kidney

text: APPwt expression

text: Homo sapiens


name: E-MTAB-12033:HEK APPwt cells, Total RNA used for RT-PCR, biological replicate 2

text: JOHANNES GUTENBERG UNIVERSITY MAINZ

text: public

text: ArrayExpress

text: human

characteristic: common name

characteristic: description

text: embryo

text: APPwt

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: kidney

text: APPwt expression

text: Homo sapiens

characteristic: title


name: E-MTAB-12033:HEK APPwt cells, Total RNA used for RT-PCR, biological replicate 3

characteristic: ENA-CHECKLIST

text: JOHANNES GUTENBERG UNIVERSITY MAINZ

text: public

text: ArrayExpress

text: human

text: embryo

characteristic: developmental stage

text: APPwt

characteristic: genotype

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: kidney

text: APPwt expression

text: Homo sapiens


name: E-MTAB-12033:HEK APPwt cells, Total RNA used for RT-PCR, biological replicate 4

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: JOHANNES GUTENBERG UNIVERSITY MAINZ

text: public

text: ArrayExpress

text: human

text: embryo

text: APPwt

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: kidney

characteristic: organism part

text: APPwt expression

characteristic: phenotype

text: Homo sapiens


name: E-MTAB-12033:HEK ut cells, mitochondrial RNA used for RT-PCR, biological replicate 2

characteristic: External Id

text: JOHANNES GUTENBERG UNIVERSITY MAINZ

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: human

text: embryo

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: kidney

text: wild type

text: Homo sapiens


name: E-MTAB-12033:HEK ut cells, mitochondrial RNA used for RT-PCT, biological replicate 1

text: JOHANNES GUTENBERG UNIVERSITY MAINZ

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: human

text: embryo

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: kidney

text: wild type

text: Homo sapiens


name: E-MTAB-12033:HEK ut cells, Total RNA used for RT-PCR, biological replicate 1

text: JOHANNES GUTENBERG UNIVERSITY MAINZ

text: public

text: ArrayExpress

text: human

characteristic: common name

characteristic: description

text: embryo

text: wild type genotype

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: kidney

text: wild type

text: Homo sapiens

characteristic: title


name: E-MTAB-12033:HEK ut cells, Total RNA used for RT-PCR, biological replicate 2

characteristic: ENA-CHECKLIST

text: JOHANNES GUTENBERG UNIVERSITY MAINZ

text: public

text: ArrayExpress

text: human

text: embryo

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: kidney

text: wild type

text: Homo sapiens


name: E-MTAB-12033:HEK ut cells, Total RNA used for RT-PCR, biological replicate 3

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: JOHANNES GUTENBERG UNIVERSITY MAINZ

text: public

text: ArrayExpress

text: human

text: embryo

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: kidney

characteristic: organism part

text: wild type

characteristic: phenotype

text: Homo sapiens


name: E-MTAB-12033:HEK ut cells, Total RNA used for RT-PCR, biological replicate 4

characteristic: External Id

text: JOHANNES GUTENBERG UNIVERSITY MAINZ

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: human

text: embryo

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: kidney

text: wild type

text: Homo sapiens


text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Swedish National Veterinary Institute (SVA)

characteristic: Submitter Id

text: E. coli O157

text: Sweden

characteristic: geographic location (country and/or sea)

text: Cattle

characteristic: host scientific name

text: Escherichia coli

text: Escherichia coli

characteristic: serotype


characteristic: ENA-CHECKLIST

text: Swedish National Veterinary Institute (SVA)

text: public

text: E. coli O157

characteristic: description

text: Sweden

text: Cattle

text: Escherichia coli

characteristic: organism

text: Escherichia coli

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E. coli O121

text: Sweden

text: Cattle

text: Escherichia coli

text: Escherichia coli


text: public

text: E. coli O26

text: Sweden

text: Cattle

text: Escherichia coli

text: Escherichia coli


text: Insitute of Oncology Research (IOR)

text: public

tag: Namespace:Insitute of Oncology Research (IOR)

text: ArrayExpress

characteristic: broker name

text: VCaP

text: prostate gland cancer cell

text: human

characteristic: common name

text: Protocols: VCaP cells were obtained from American Type Culture Collection and maintained in their media. DMEM [ATCC, cat #30-2002] supplemented with 10% fetal bovine serum (FBS, Biowest, cat #S181B) and 1% Pen/Strep (Thermo Fisher, cat #15070063) was used for VCaP. Cells were regularly checked for mycoplasm contamination using MycoAlert Mycoplasma detection kit. VCaP with stable knockdown of MAT2A were generated by viral infection of control (Ctrl) and MAT2A shRNAs (Sh1 and Sh2) [Sigma-Aldrich], followed by subsequent selection with Puromycin [Sigma-Aldrich]. Collection of 5 million cells in PBS RNA was isolated from RWPE cells in three independent replicates, marked with Illumina total prep 96 RNA amplification kit (Ambion). RNA sequencing was performed using Next Ultra II Directional RNA Library Prep Kit for Illumina starting from 800ng of total RNA each sample and sequenced on the Illumina NextSeq500 with single-end, 75 base pair long reads

text: prostate cancer

characteristic: disease

text: wild type genotype

characteristic: genotype

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: prostate gland

characteristic: organism part

text: Homo sapiens


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Mario Negri Institute for pharmacological research (IRCCS)

text: ArrayExpress

text: epithelial cell

text: human

text: Protocols: Collection of 5 million cells in PBS RNA was isolated from RWPE cells in three independent replicates, marked with Illumina total prep 96 RNA amplification kit (Ambion). RNA sequencing was performed using Next Ultra II Directional RNA Library Prep Kit for Illumina starting from 800ng of total RNA each sample and sequenced on the Illumina NextSeq500 with single-end, 75 base pair long reads

text: normal

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: prostate gland

text: wild type

characteristic: phenotype

text: Homo sapiens


characteristic: ENA-CHECKLIST

text: Mario Negri Institute for pharmacological research (IRCCS)

text: public

characteristic: Submitter Id

text: ArrayExpress

text: epithelial cell

text: human

characteristic: description

text: normal

text: wild type genotype

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: prostate gland

text: wild type

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: ArrayExpress

characteristic: broker name

text: epithelial cell

text: human

text: normal

text: pCMW57.1-MAT2A; pCEFL-ha-ERG

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: prostate gland

text: MAT2A and ERG overexpression

text: Homo sapiens

characteristic: title


text: public

text: ArrayExpress

text: epithelial cell

text: human

characteristic: common name

text: normal

characteristic: disease

text: pCEFL-ha-ERG

characteristic: genotype

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: prostate gland

text: ERG overexpression

text: Homo sapiens


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: epithelial cell

text: human

text: normal

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: prostate gland

characteristic: organism part

text: MAT2A overexpression

text: Homo sapiens


text: Institute of Oncology research (IOR)

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Institute of Oncology research (IOR)

text: ArrayExpress

text: VCaP

text: epithelial cell

text: human

text: Protocols: Collection of 50000 cells per replicate and resuspension of the pellet in TRIzol buffer - Resuspension of nuclei in the transposition reaction mix- Incubation of the transposition reaction- Purification using Qiagen MinElute PCR Purification Kit- Elution of transposed DNA in Elution BufferRefer to DOI: 10.1002/0471142727.mb2129s109 - Amplification of DNA fragments - Purification of amplified library using Qiagen MinElute PCR Purification Kit

text: prostate cancer

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: prostate gland

characteristic: replicate

text: Homo sapiens


characteristic: ENA-CHECKLIST

text: JOHANNES GUTENBERG UNIVERSITY MAINZ

text: public

characteristic: Submitter Id

text: ArrayExpress

text: HEK293-T-REx cell

text: epithelial cell

text: human

text: Protocols: HEK pTRMT10C cells and the control cells (Ctl) were seeded two days prior to the RNA isolation, each in three wells to test three different tetracycline concentrations. The next day, the media was replaced with growth medium, containing 0 µg/mL, 0.1 µg/mL and 1 µg/mL tetracycline. After incubation for 24 hours at 37°C in a humidified incubator containing 5% CO2, RNA isolation was performed. pTRMT10C cells: T-Rex-293 (Invitrogen, R71007) (Ctl cells) were transfected with a pcDNA™5/TO plasmid coding for full-length TRMT10C in frame with a FLAG-tag. The combination of T-Rex-293 and the TRTM10C plasmid enables a tetracycline-inducible overexpression of TRMT10C in dependece of the tetracycline concentration. Control T-Rex-293 (Ctl) cells were cultured in DMEM supplemented with 10% heat-inactivated FCS, 50 Units/mL penicillin, 50 µg/mL streptomycin and 5 µg/mL blasticidin (Thermo Fisher Scientific, Cat.-No: R21001). Medium of pTRMT10C cells additionally contained 100 µg/mL hygromycin (Invitrogen, 10687010) for selecting the pcDNA™5/TO plasmid. 24 hours before the isolation of total RNA pTRMT10C as well as T-Rex-293 cells were incubated with 0 µg/mL, 0,1 µg/mL and 1 µg/mL tetracycline (Sigma-Aldrich, T7660). Cells were harvested at a confluency of 70-80% in 1 mL TRI Reagent®. Dissolution of the cells was supported by vortexing and incubation at room temperature for 5 min. Next 200 µL of chloroform (Sigma-Aldrich, C2432) was added and mixed thoroughly. After a second incubation step (10 min) at 21 °C, the cell suspension was centrifuged (16 000 x g, 4 °C, 15 min). The upper aqueous phase was taken and mixed in a 1:1 ratio with isopropanol (ROTH, 7343.1) and 1 µL glycogen (Thermo Scientific, R0551) was added, incubated at 21 °C (5 min) and centrifuged (16 000 x g, 4 °C, 10 min). The RNA pellet was washed twice with ice-cold 75% ethanol including centrifugation (16 000 x g, 4 °C, 5 min). RNA was reconstituted in 10-20 µL nuclease-free water (Invitrogen, AM9930). RNA concentrations were determined using UV-VIS spectrophotometer Nanodrop 2000 (Thermo Fisher Scientific, Waltham, USA). Total RNA was incubated with DNase I (Zymo Research, E1010) and 10x DNase digestion Buffer (Zymo Research, E1010) at 21 °C (15 min) to eliminate any existing DNA contamination and a second phenol-chloroform extraction was performed as described above. For reverse transcription 2 µg RNA, 0.5 mM dNTPs (Thermo Scientific, R0192), water and a target-specific RT primer (see Supplementary Table S1) (2.5 µM) were mixed and incubated for 5 min at 75 °C and afterwards 5 min on ice. Next 10 U/µL SuperScript-IV (Invitrogen, 18090050), dithiothreitol (DTT) and 5x RT Buffer and were added, mixed and incubated for 2 hours at 50 °C. In the end, the enzyme was inactivated at 80 °C for 15 min. The RT Primer contained a target-specific sequence complementary to ND5 mRNA and a primer-binding site for indexing with the NEBNext® Multiplex Small RNA Library Prep Kit for Illumina® (New England Biolabs, #E7560S) primers afterwards during polymerase chain reaction (PCR). PCR was conducted with 0.04 U/µL Platinum SuperFi (Invitrogen, 12351010), 5x SuperFi buffer (Invitrogen, 12351010), 0.2 mM dNTPs, 5x GC Enhancer (Invitrogen, 12351010), 0.5 µM custom-designed forward primer (see Supplementary Table S1) and reverse primers of the NEBNext® Multiplex Small RNA Library Prep Kit for Illumina®. The custom p5 PCR primer contained flow cell binding sequence, sequencing primer site 1 and a target-specific sequence complementary to ND5 cDNA. The commercial i7 index primers introduced individual barcodes for each sample, contained the sequencing primer site 2 and the second flow cell binding sequence. PCR was started in Thermocycler peqSTAR (VWR Peqlab), including an initial denaturation step at 98 °C (2 min), 30 cycles at 98 °C (15 min), 63 °C (1 min) and 72 °C (2 min), and a final extension step at 72°C (5 min). PCR products were purified on a 10% denaturing polyacrylamide gel by size selection and the expected full-length product was excised. Finally, prepared libraries were quality controlled for adapter dimer formation on an Agilent 4200 TapeStation (Agilent, Santa Clara, United States).

characteristic: description

text: embryo stage

characteristic: developmental stage

text: wild type genotype

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: kidney

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: JOHANNES GUTENBERG UNIVERSITY MAINZ

text: public

text: ArrayExpress

characteristic: broker name

text: HEK293-T-REx cell

text: epithelial cell

text: human

characteristic: common name

text: embryo stage

text: tetracycline-inducible TRMT10C expressing

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: kidney

text: Homo sapiens

characteristic: title


E-MTAB-12048:Sample 49

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-12048:Sample 49

tag: Namespace:Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen  Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen

unit: week

characteristic: age

text: ArrayExpress

text: house mouse

text: Protocols: The mice were fasted overnight (11 hours) prior to liver isolation, and livers were kept at -80°C until analysis. For eight weeks, female C57BL/6JRj mice (seven weeks old at the start of treatment) received, by subcutaneous injections, either a glucagon receptor antibody (GCGR Ab, REGN1193, 10 mg/kg body weight; Regeneron; IC50: 3.5 pM (Okamoto et al., 2015)) or a control antibody (Ctl Ab, REGN1945, 10 mg/kg body weight; Regeneron). The antibodies were administered once weekly (Wednesdays at 8 AM). The last doses of GCGR Ab and Ctl Ab were given one week prior to termination. Raw sequencing data were processed using bcl2fastq software version 2.20.0 (Illumina). Total DNA/RNA was purified by an AllPrep DNA/RNA Minikit (80204, QIAgen) according to manufacturer’s instructions and quality tested using a 2100 Bioanalyzer instrument (Agilent Genomics). The extract was DNase treated with RNeasy Minikit (74106, QIAgen) and RNase-Free DNase set (79254, QIAgen) according to manufacturer’s protocol. All the above-mentioned processes were performed on a QIACube machine (QIAgen). Library preparation was done using TruSeq Stranded Total RNA Library Prep Gold (20020599, Illumina) and IDT for Illumina-TruSeq RNA UD Indexes (20022371, Illumina) according to the manufacturer’s instructions. Some samples (input <100 ng) were prepared with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB-E7760S, New England Biolabs), NEBNext rRNA Depletion Kit 24 with beads (NEB-E7765, New England Biolabs), and NEBNext Multiplex Oligos for Illumina (E7600S, New England Biolabs).

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: liver

text: Mus musculus

text: female

characteristic: sex

characteristic: strain

text: Sample 49


E-MTAB-12048:Sample 50

text: Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12048:Sample 50

text: ArrayExpress

text: house mouse

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: liver

characteristic: organism part

text: Mus musculus

text: female

text: Sample 50


E-MTAB-12048:Sample 51

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-12048:Sample 51

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

characteristic: description

text: wild type genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: liver

text: Mus musculus

text: female

text: Sample 51


E-MTAB-12048:Sample 52

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-12048:Sample 52

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: liver

text: Mus musculus

text: female

text: Sample 52


E-MTAB-12048:Sample 57

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-12048:Sample 57

characteristic: age

text: ArrayExpress

text: house mouse

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: liver

text: Mus musculus

text: female

text: Sample 57

characteristic: title


E-MTAB-12048:Sample 58

characteristic: INSDC first public

text: public

text: E-MTAB-12048:Sample 58

text: ArrayExpress

text: house mouse

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: liver

text: Mus musculus

text: female

characteristic: sex

characteristic: strain

text: Sample 58


E-MTAB-12048:Sample 59

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12048:Sample 59

text: ArrayExpress

text: house mouse

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: liver

characteristic: organism part

text: Mus musculus

text: female

text: Sample 59


E-MTAB-12048:Sample 60

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-12048:Sample 60

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

characteristic: description

text: wild type genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: liver

text: Mus musculus

text: female

text: Sample 60


E-MTAB-12048:Sample 65

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-12048:Sample 65

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: liver

text: Mus musculus

text: female

text: Sample 65


E-MTAB-12048:Sample 66

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-12048:Sample 66

characteristic: age

text: ArrayExpress

text: house mouse

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: liver

text: Mus musculus

text: female

text: Sample 66

characteristic: title


E-MTAB-12048:Sample 67

characteristic: INSDC first public

text: public

text: E-MTAB-12048:Sample 67

text: ArrayExpress

text: house mouse

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: liver

text: Mus musculus

text: female

characteristic: sex

characteristic: strain

text: Sample 67


E-MTAB-12048:Sample 68

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12048:Sample 68

text: ArrayExpress

text: house mouse

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: liver

characteristic: organism part

text: Mus musculus

text: female

text: Sample 68


E-MTAB-12048:Sample 73

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-12048:Sample 73

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

characteristic: description

text: wild type genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: liver

text: Mus musculus

text: female

text: Sample 73


E-MTAB-12048:Sample 74

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-12048:Sample 74

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: liver

text: Mus musculus

text: female

text: Sample 74


E-MTAB-12048:Sample 75

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-12048:Sample 75

characteristic: age

text: ArrayExpress

text: house mouse

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: liver

text: Mus musculus

text: female

text: Sample 75

characteristic: title


E-MTAB-12048:Sample 76

characteristic: INSDC first public

text: public

text: E-MTAB-12048:Sample 76

text: ArrayExpress

text: house mouse

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: liver

text: Mus musculus

text: female

characteristic: sex

characteristic: strain

text: Sample 76


text: Royal Botanic Gardens, Kew

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Royal Botanic Gardens, Kew

characteristic: collected by

text: not provided

characteristic: collection date

text: LD84 - PC866

characteristic: collectorNo

text: not provided

characteristic: geographic location (country and/or sea)

characteristic: isolation source

text: Hallianthus sp. LD84 - PC866

text: Hallianthus sp. LD84 - PC866

text: LD84 PC866 (NBG)

characteristic: specimen voucher

text: Hallianthus sp. - LD84 PC866 (NBG)


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Royal Botanic Gardens, Kew

text: public

characteristic: Submitter Id

text: Unmounted

characteristic: bio material

text: Vorontsova, M.S.

text: not provided

text: spermatophyte root

characteristic: organism

text: spermatophyte root

text: Vorontsova, M.S. 1468 (K); Unmounted

text: Oplismenus sp. - Vorontsova, M.S. 1468 (K); Unmounted


text: Technical University of Denmark

characteristic: INSDC center name

characteristic: INSDC first public

text: public

tag: Namespace:Technical University of Denmark

text: human-gut

characteristic: broad-scale environmental context

text: human

characteristic: common name

characteristic: description

text: feces

characteristic: environmental medium

text: Sweden

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: gut

characteristic: local environmental context

text: Homo sapiens

characteristic: project name

text: Homo sapiens

text: metagenome

characteristic: sequencing method

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Baldridge_1_TACACGATCTAC_ACGACGTCTTAG_S12

tag: Namespace:WASHINGTON UNIVERSITY SCHOOL OF MEDICINE

text: house mouse

text: Day-0_Pre-transplant_Pre-transplant-Group

text: Mus musculus

text: Mus musculus


characteristic: External Id

text: WASHINGTON UNIVERSITY SCHOOL OF MEDICINE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Baldridge_2_TACACGATCTAC_GGAATCTGAGCC_S13

characteristic: Submitter Id

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus


text: public

text: Baldridge_3_TACACGATCTAC_GGCACTACCAAG_S14

text: house mouse

characteristic: common name

characteristic: description

text: Mus musculus

text: Mus musculus


text: public

text: Baldridge_4_TACACGATCTAC_TAAGCGCAGCAC_S15

text: house mouse

text: Mus musculus

text: Mus musculus


text: public

text: Baldridge_5_TACACGATCTAC_GTGCCTCCTACT_S16

text: house mouse

text: Mus musculus

text: Mus musculus


text: public

text: Baldridge_6_TACACGATCTAC_CGAAATCCGGGT_S17

text: house mouse

text: Mus musculus

text: Mus musculus


text: public

text: Baldridge_7_TACACGATCTAC_TTCATACGGACG_S18

text: house mouse

text: Mus musculus

text: Mus musculus


text: public

text: Baldridge_8_TACACGATCTAC_GATTCCTAATCG_S19

text: house mouse

text: Mus musculus

text: Mus musculus


text: public

text: Baldridge_9_GCACACACGTTA_ACGACGTCTTAG_S20

text: house mouse

text: Mus musculus

text: Mus musculus


text: public

text: Baldridge_10_GCACACACGTTA_GGAATCTGAGCC_S21

text: house mouse

text: Mus musculus

text: Mus musculus


text: public

text: Baldridge_11_GCACACACGTTA_GGCACTACCAAG_S22

text: house mouse

text: Mus musculus

text: Mus musculus


text: public

text: Baldridge_12_GCACACACGTTA_TAAGCGCAGCAC_S23

text: house mouse

text: Mus musculus

text: Mus musculus


text: public

text: Baldridge_13_GCACACACGTTA_GTGCCTCCTACT_S24

text: house mouse

text: Mus musculus

text: Mus musculus


text: public

text: Baldridge_14_GCACACACGTTA_CGAAATCCGGGT_S25

text: house mouse

text: Mus musculus

text: Mus musculus


text: public

text: Baldridge_15_GCACACACGTTA_TTCATACGGACG_S26

text: house mouse

text: Mus musculus

text: Mus musculus


text: public

text: Baldridge_16_GCACACACGTTA_GATTCCTAATCG_S27

text: house mouse

text: Mus musculus

text: Mus musculus


text: public

text: Baldridge_17_CACGCCATAATG_ACGACGTCTTAG_S28

text: house mouse

text: Mus musculus

text: Mus musculus


text: WASHINGTON UNIVERSITY SCHOOL OF MEDICINE

text: public

text: Baldridge_18_CACGCCATAATG_GGAATCTGAGCC_S29

text: house mouse

text: Mus musculus

text: Mus musculus


text: public

text: Baldridge_19_CACGCCATAATG_GGCACTACCAAG_S30

text: house mouse

text: Mus musculus

text: Mus musculus


text: public

text: Baldridge_20_CACGCCATAATG_TAAGCGCAGCAC_S31

text: house mouse

text: Mus musculus

text: Mus musculus


text: public

text: Baldridge_21_CACGCCATAATG_GTGCCTCCTACT_S32

text: house mouse

text: Mus musculus

text: Mus musculus


text: public

text: Baldridge_22_CACGCCATAATG_CGAAATCCGGGT_S33

text: house mouse

text: Mus musculus

text: Mus musculus


text: public

text: Baldridge_23_CACGCCATAATG_TTCATACGGACG_S34

text: house mouse

text: Mus musculus

text: Mus musculus


text: public

text: Baldridge_24_CACGCCATAATG_GATTCCTAATCG_S35

text: house mouse

text: Mus musculus

text: Mus musculus