E-MTAB-12573:2D PD replicate 1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-12573:2D PD replicate 1

text: ArrayExpress

text: cardiocyte

text: human

characteristic: description

text: embryo

text: normal

text: wild type genotype

characteristic: genotype

text: 2D adherent cell culture

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

text: embryonic stem cell

text: Homo sapiens

text: 2D PD replicate 1

characteristic: title


E-MTAB-12573:2D PD replicate 2

status: PUBLIC

tag: attribute

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12573:2D PD replicate 2

tag: Namespace:Department of Biomedical Sciences, University of Copenhagen, Denmark

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: cardiocyte

text: human

characteristic: common name

text: Protocols: Cells from 2D culture were scraped in RLT buffer (QIAGEN RNeasy kit). Organoids were dissolved in RLT buffer and passed 10-times through a 21G needle. Note: the \"replicate 1\" of 2D control and PD have 3 data files, corresponding to 3 differentiation runs carried out at the same time, e.g. derived from the same cell populations. QIAGEN RNeasy kits (Mini for 2D culture and Micro for organoids) according to manufacturer's handbook. Library was prepared with NEBNext Ultra II RNA Library Prep Kit (New England BioLabs). For the 2D cultures, 500 ng of RNA were used and 5 cycles of PCR amplification were performed, whereas for the organoids, 120 ng RNA were used and 9 cycles of PCR amplification were performed. AMPure XP (Beckman Coulter) was used for purification. Fragment Analyzer (Agilent) was used for quality assessment.

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

text: 2D adherent cell culture

characteristic: growth condition

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: 2D PD replicate 2


E-MTAB-12573:2D PD replicate 3

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Department of Biomedical Sciences, University of Copenhagen, Denmark

text: public

text: E-MTAB-12573:2D PD replicate 3

text: ArrayExpress

text: cardiocyte

text: human

characteristic: description

text: embryo

text: normal

text: wild type genotype

characteristic: genotype

text: 2D adherent cell culture

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

text: embryonic stem cell

text: Homo sapiens

text: 2D PD replicate 3

characteristic: title


E-MTAB-12573:Organoid PD replicate 1

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12573:Organoid PD replicate 1

text: ArrayExpress

text: cardiocyte

text: human

text: embryo

text: normal

text: wild type genotype

text: organoid

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: embryonic stem cell

text: Homo sapiens

text: Organoid PD replicate 1


E-MTAB-12573:Organoid PD replicate 2

text: public

characteristic: SRA accession

text: E-MTAB-12573:Organoid PD replicate 2

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: cardiocyte

text: human

characteristic: common name

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

text: organoid

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: embryonic stem cell

text: Homo sapiens

text: Organoid PD replicate 2


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Department of Life Science and Medecine

text: public

tag: Namespace:Department of Life Science and Medecine

characteristic: batch

text: Melanoma

text: human

text: NRAS mutant melanoma cell line IPC298

characteristic: inhibitors

text: Homo sapiens

text: Homo sapiens

text: Female

characteristic: sex

characteristic: time of treatment


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Department of Life Science and Medecine

text: public

text: Melanoma

text: human

text: NRAS mutant melanoma cell line IPC298 treated with CDK4/6i (Palbociclib) 1?M and MEKi (Bnimetinib) 16 nM for 33 days

characteristic: description

text: CDK4/6i (Palbociclib) 1?M, MEKi (Bnimetinib) 16 nM

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Female

characteristic: title


text: Department of Life Science and Medecine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: MELJUSO

text: Melanoma

text: human

text: NRAS mutant melanoma cell line MELJUSO

text: Homo sapiens

text: Homo sapiens

text: Female


text: Department of Life Science and Medecine

text: public

characteristic: Submitter Id

text: MELJUSO

text: Melanoma

text: human

characteristic: common name

text: NRAS mutant melanoma cell line MELJUSO treated with CDK4/6i (Palbociclib) 1?M and MEKi (Bnimetinib) 16 nM for 33 days

text: CDK4/6i (Palbociclib) 1?M, MEKi (Bnimetinib) 110 nM

text: Homo sapiens

text: Homo sapiens

text: Female


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Department of Life Science and Medecine

text: public

text: Melanoma

text: human

text: NRAS mutant melanoma cell line SKMEL30

text: Homo sapiens

text: Homo sapiens

text: Male


text: Department of Life Science and Medecine

text: public

characteristic: batch

text: Melanoma

text: human

text: NRAS mutant melanoma cell line SKMEL30 treated with CDK4/6i (Palbociclib) 1?M and MEKi (Bnimetinib) 16 nM for 33 days

text: CDK4/6i (Palbociclib) 1?M, MEKi (Bnimetinib) 35 nM

characteristic: inhibitors

text: Homo sapiens

text: Homo sapiens

text: Male


text: Department of Life Science and Medecine

text: public

text: Melanoma

text: human

text: NRAS mutant melanoma cell line IPC298 treated with CDK4/6i (Palbociclib) 1?M and MEKi (Bnimetinib) 16 nM for 4 days

text: Homo sapiens

text: Homo sapiens

text: Female

characteristic: sex

characteristic: time of treatment


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Department of Life Science and Medecine

text: public

text: Melanoma

text: human

text: NRAS mutant melanoma cell line SKMEL30 treated with CDK4/6i (Palbociclib) 1?M and MEKi (Bnimetinib) 16 nM for 4 days

text: Homo sapiens

text: Homo sapiens

text: Male


text: Department of Life Science and Medecine

text: public

text: MELJUSO

text: Melanoma

text: human

text: NRAS mutant melanoma cell line MELJUSO treated with CDK4/6i (Palbociclib) 1?M and MEKi (Bnimetinib) 16 nM for 4 days

characteristic: description

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Female


text: Department of Life Science and Medecine

characteristic: INSDC center name

text: public

text: MELJUSO

text: Melanoma

text: human

text: Homo sapiens

text: Homo sapiens

text: Female

characteristic: title


text: Department of Life Science and Medecine

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Melanoma

text: human

text: NRAS mutant melanoma cell line IPC298 treated with CDK4/6i (Palbociclib) 1?M and MEKi (Bnimetinib) 16 nM for 14 days

text: Homo sapiens

text: Homo sapiens

text: Female


text: Department of Life Science and Medecine

text: public

characteristic: INSDC status

characteristic: SRA accession

text: MELJUSO

text: Melanoma

text: human

text: NRAS mutant melanoma cell line MELJUSO treated with CDK4/6i (Palbociclib) 1?M and MEKi (Bnimetinib) 16 nM for 14 days

text: Homo sapiens

text: Homo sapiens

text: Female


text: Department of Life Science and Medecine

text: public

characteristic: Submitter Id

text: Melanoma

text: human

characteristic: common name

text: NRAS mutant melanoma cell line SKMEL30 treated with CDK4/6i (Palbociclib) 1?M and MEKi (Bnimetinib) 16 nM for 14 days

text: Homo sapiens

text: Homo sapiens

text: Male


NIP

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: NCGR

text: public

text: NIP

text: Asian cultivated rice

text: Rice Nipponbare

text: Oryza sativa

text: Oryza sativa

text: NIP


E-MTAB-12619:Sample 1

text: public

text: E-MTAB-12619:Sample 1

text: ArrayExpress

characteristic: broker name

text: cardiocyte

text: human

text: Protocols: Single-cell RNA-seq was performed from cryopreserved cells. TrypLE Select was used to detach and singularize the cells. Cells were frozen in KnockOut Serum Replacement with 10 % DMSO and thawed in DMEM/F-12 with 10 % KnockOut Serum Replacement. 10x Genomics 3' v3.1 kit 10x Genomics 3' v3.1 kit

text: embryo stage

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: heart

characteristic: organism part

text: embryonic stem cell

characteristic: progenitor cell type

text: inner cell mass

characteristic: progenitor organism part

text: Homo sapiens

text: Sample 1


E-MTAB-12619:Sample 2

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-12619:Sample 2

text: ArrayExpress

text: cardiocyte

text: human

characteristic: description

text: embryo stage

text: normal

text: wild type genotype

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: heart

text: embryonic stem cell

text: inner cell mass

text: Homo sapiens

text: Sample 2

characteristic: title


E-MTAB-12619:Sample 3

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12619:Sample 3

characteristic: Submitter Id

text: ArrayExpress

text: cardiocyte

text: human

characteristic: common name

text: embryo stage

text: normal

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: heart

text: embryonic stem cell

text: inner cell mass

text: Homo sapiens

text: Sample 3


E-MTAB-12619:Sample 4

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-12619:Sample 4

text: ArrayExpress

characteristic: broker name

text: cardiocyte

text: human

text: embryo stage

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: heart

text: embryonic stem cell

text: inner cell mass

text: Homo sapiens

text: Sample 4


E-MTAB-12619:Sample 5

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-12619:Sample 5

text: ArrayExpress

text: cardiocyte

text: human

text: embryo stage

text: normal

text: wild type genotype

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: heart

characteristic: organism part

text: embryonic stem cell

characteristic: progenitor cell type

text: inner cell mass

characteristic: progenitor organism part

text: Homo sapiens

text: Sample 5


E-MTAB-12638:D0 - WT1

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-12638:D0 - WT1

tag: Namespace:Institut Necker-Enfants Malades, INSERM U1151/CNRS UMR 8253, Universite Paris Descartes, Sorbonne Paris Cite, 75993 Paris Cedex 14, France.

unit: week

characteristic: age

text: ArrayExpress

text: naive B cell

text: house mouse

text: Protocols: The transcriptomic impact of Hells loss-of-function was quantified by RNA sequencing of sample from conditional knockout (Mb1-Cre+/WT HellsF/F) and littermate control mice (Mb1-CreWT/WT HellsF/F). Three time points were chosen to follow the kinetic: day 0 for naïve B cells collection, day 7 and day 14 post NP-CGG intraperitoneal immunization for germinal center B cells collection. At each time point, 4 mice of each genotype were used. Live cells were FACS sorted (AriaIII) using surface markers as follows: B220+CD21+CD23+ for naïve B cells, and B220+CD95+GL7+ for germinal center B cells. Due to their low frequency, germinal center B cells were FACS-sorted twice using the same gating strategy. Collected samples were lysed and processed for RNA extraction. Mice were immunized intraperitoneally (i.p.) with 100µg of NP-CGG (4-hydroxy-3-nitrophenylacetyl-Chicken Gamma Globulin, Biosearch Technologies) dissolved in PBS and adsorbed on Alhydrogel aluminium hydroxide following manufacturer’s instructions (Invivogen) Total RNA were isolated using the RNeasy Kit (QIAGEN) including a DNAse treatment step. RNA integrity and concentration were assessed by capillary electrophoresis using Fragment Analyzer (Agilent Technologies). RNAseq libraries were prepared starting from 188.3ng, 139.8 or 29.3ng of total RNA (3 groups) using the Universal Plus mRNA-Seq kit (Nugen) as recommended by the manufacturer. With this kit, stranded RNAseq libraries can be prepared from 10 ng to 1µg of total RNA. Briefly, the mRNA were captured with polyA+ magnetic beads form the total RNA. The mRNA were fragmented chemically. Single strand and second strand cDNA were produced and then ligated to Illumina compatible adaptators with Unique Dual Index. The cDNA produced were PCR amplified after an initial test to evaluate the suitable number of PCR cycle to apply to each sample. To produce oriented or ‘stranded’ RNAseq libraries, a final step of strand selection was performed.

characteristic: description

text: adult

text: HellsF/F Mb1Cre-

text: 266 - 5

characteristic: individual

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spleen

text: wild type phenotype

characteristic: phenotype

text: Mus musculus

text: female

characteristic: sex

text: C57BL/6J x 129Sv

characteristic: strain

text: D0 - WT1

characteristic: title


E-MTAB-12638:D0 - WT2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Institut Necker-Enfants Malades, INSERM U1151/CNRS UMR 8253, Universite Paris Descartes, Sorbonne Paris Cite, 75993 Paris Cedex 14, France.

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12638:D0 - WT2

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: naive B cell

text: house mouse

characteristic: common name

text: adult

characteristic: developmental stage

text: HellsF/F Mb1Cre-

characteristic: genotype

text: 344 - 8bis

text: not applicable

text: Mus musculus

text: Mus musculus

text: spleen

text: wild type phenotype

text: Mus musculus

text: male

text: C57BL/6J x 129Sv

text: D0 - WT2


E-MTAB-12638:D0 - WT3

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-12638:D0 - WT3

characteristic: age

text: ArrayExpress

text: naive B cell

text: house mouse

characteristic: description

text: adult

text: HellsF/F Mb1Cre-

text: 345 - 10

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: spleen

characteristic: organism part

text: wild type phenotype

text: Mus musculus

text: male

text: C57BL/6J x 129Sv

text: D0 - WT3


E-MTAB-12638:D0 - WT4

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-12638:D0 - WT4

text: ArrayExpress

text: naive B cell

text: house mouse

text: adult

text: HellsF/F Mb1Cre-

text: 325 - 11

characteristic: individual

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spleen

text: wild type phenotype

characteristic: phenotype

text: Mus musculus

text: male

characteristic: sex

text: C57BL/6J x 129Sv

characteristic: strain

text: D0 - WT4

characteristic: title


E-MTAB-12638:D14 - WT1

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12638:D14 - WT1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: germinal center B cell

text: house mouse

characteristic: common name

text: adult

characteristic: developmental stage

text: HellsF/F Mb1Cre-

characteristic: genotype

text: 193 - J14-18

text: not applicable

text: Mus musculus

text: Mus musculus

text: spleen

text: wild type phenotype

text: Mus musculus

text: female

text: C57BL/6J x 129Sv

text: D14 - WT1


E-MTAB-12638:D14 - WT2

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-12638:D14 - WT2

characteristic: age

text: ArrayExpress

text: germinal center B cell

text: house mouse

characteristic: description

text: adult

text: HellsF/F Mb1Cre-

text: 307 - J14-22

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: spleen

characteristic: organism part

text: wild type phenotype

text: Mus musculus

text: female

text: C57BL/6J x 129Sv

text: D14 - WT2


E-MTAB-12638:D14 - WT3

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-12638:D14 - WT3

text: ArrayExpress

text: germinal center B cell

text: house mouse

text: adult

text: HellsF/F Mb1Cre-

text: 350 - J14-25

characteristic: individual

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spleen

text: wild type phenotype

characteristic: phenotype

text: Mus musculus

text: male

characteristic: sex

text: C57BL/6J x 129Sv

characteristic: strain

text: D14 - WT3

characteristic: title


E-MTAB-12638:D14 - WT4

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12638:D14 - WT4

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: germinal center B cell

text: house mouse

characteristic: common name

text: adult

characteristic: developmental stage

text: HellsF/F Mb1Cre-

characteristic: genotype

text: 295 - J14-29

text: not applicable

text: Mus musculus

text: Mus musculus

text: spleen

text: wild type phenotype

text: Mus musculus

text: male

text: C57BL/6J x 129Sv

text: D14 - WT4


E-MTAB-12638:D7 - WT1

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-12638:D7 - WT1

characteristic: age

text: ArrayExpress

text: germinal center B cell

text: house mouse

characteristic: description

text: adult

text: HellsF/F Mb1Cre-

text: 189 - J7-12

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: spleen

characteristic: organism part

text: wild type phenotype

text: Mus musculus

text: male

text: C57BL/6J x 129Sv

text: D7 - WT1


E-MTAB-12638:D7 - WT2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-12638:D7 - WT2

text: ArrayExpress

text: germinal center B cell

text: house mouse

text: adult

text: HellsF/F Mb1Cre-

text: 300 - J7-2

characteristic: individual

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spleen

text: wild type phenotype

characteristic: phenotype

text: Mus musculus

text: female

characteristic: sex

text: C57BL/6J x 129Sv

characteristic: strain

text: D7 - WT2

characteristic: title


E-MTAB-12638:D7 - WT3

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12638:D7 - WT3

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: germinal center B cell

text: house mouse

characteristic: common name

text: adult

characteristic: developmental stage

text: HellsF/F Mb1Cre-

characteristic: genotype

text: 305 - J7-4

text: not applicable

text: Mus musculus

text: Mus musculus

text: spleen

text: wild type phenotype

text: Mus musculus

text: female

text: C57BL/6J x 129Sv

text: D7 - WT3


E-MTAB-12638:D7 - WT4

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-12638:D7 - WT4

characteristic: age

text: ArrayExpress

text: germinal center B cell

text: house mouse

characteristic: description

text: adult

text: HellsF/F Mb1Cre-

text: 290 - J7-5

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: spleen

characteristic: organism part

text: wild type phenotype

text: Mus musculus

text: male

text: C57BL/6J x 129Sv

text: D7 - WT4


E-MTAB-12638:D0 - KO1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-12638:D0 - KO1

text: ArrayExpress

text: naive B cell

text: house mouse

text: adult

text: HellsF/F Mb1Cre+

text: 287 - 2

characteristic: individual

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spleen

text: Mb1 Cre-driven knockout of Hells

characteristic: phenotype

text: Mus musculus

text: male

characteristic: sex

text: C57BL/6J x 129Sv

characteristic: strain

text: D0 - KO1

characteristic: title


E-MTAB-12638:D0 - KO2

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12638:D0 - KO2

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: naive B cell

text: house mouse

characteristic: common name

text: adult

characteristic: developmental stage

text: HellsF/F Mb1Cre+

characteristic: genotype

text: 278 - 6

text: not applicable

text: Mus musculus

text: Mus musculus

text: spleen

text: Mb1 Cre-driven knockout of Hells

text: Mus musculus

text: female

text: C57BL/6J x 129Sv

text: D0 - KO2


E-MTAB-12638:D0 - KO3

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-12638:D0 - KO3

characteristic: age

text: ArrayExpress

text: naive B cell

text: house mouse

characteristic: description

text: adult

text: HellsF/F Mb1Cre+

text: 340 - 8

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: spleen

characteristic: organism part

text: Mb1 Cre-driven knockout of Hells

text: Mus musculus

text: male

text: C57BL/6J x 129Sv

text: D0 - KO3


E-MTAB-12638:D0 - KO4

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-12638:D0 - KO4

text: ArrayExpress

text: naive B cell

text: house mouse

text: adult

text: HellsF/F Mb1Cre+

text: 440 - 9

characteristic: individual

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spleen

text: Mb1 Cre-driven knockout of Hells

characteristic: phenotype

text: Mus musculus

text: female

characteristic: sex

text: C57BL/6J x 129Sv

characteristic: strain

text: D0 - KO4

characteristic: title


E-MTAB-12638:D14 - KO1

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12638:D14 - KO1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: germinal center B cell

text: house mouse

characteristic: common name

text: adult

characteristic: developmental stage

text: HellsF/F Mb1Cre+

characteristic: genotype

text: 186 - J14-15

text: not applicable

text: Mus musculus

text: Mus musculus

text: spleen

text: Mb1 Cre-driven knockout of Hells

text: Mus musculus

text: male

text: C57BL/6J x 129Sv

text: D14 - KO1


E-MTAB-12638:D14 - KO2

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-12638:D14 - KO2

characteristic: age

text: ArrayExpress

text: germinal center B cell

text: house mouse

characteristic: description

text: adult

text: HellsF/F Mb1Cre+

text: 192 - J14-17

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: spleen

characteristic: organism part

text: Mb1 Cre-driven knockout of Hells

text: Mus musculus

text: female

text: C57BL/6J x 129Sv

text: D14 - KO2


E-MTAB-12638:D14 - KO3

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-12638:D14 - KO3

text: ArrayExpress

text: germinal center B cell

text: house mouse

text: adult

text: HellsF/F Mb1Cre+

text: 351 - J14-26

characteristic: individual

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spleen

text: Mb1 Cre-driven knockout of Hells

characteristic: phenotype

text: Mus musculus

text: female

characteristic: sex

text: C57BL/6J x 129Sv

characteristic: strain

text: D14 - KO3

characteristic: title


E-MTAB-12638:D14 - KO4

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12638:D14 - KO4

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: germinal center B cell

text: house mouse

characteristic: common name

text: adult

characteristic: developmental stage

text: HellsF/F Mb1Cre+

characteristic: genotype

text: 394 - J14-28

text: not applicable

text: Mus musculus

text: Mus musculus

text: spleen

text: Mb1 Cre-driven knockout of Hells

text: Mus musculus

text: male

text: C57BL/6J x 129Sv

text: D14 - KO4


E-MTAB-12638:D7 - KO1

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-12638:D7 - KO1

characteristic: age

text: ArrayExpress

text: germinal center B cell

text: house mouse

characteristic: description

text: adult

text: HellsF/F Mb1Cre+

text: 187 - J7-11

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: spleen

characteristic: organism part

text: Mb1 Cre-driven knockout of Hells

text: Mus musculus

text: male

text: C57BL/6J x 129Sv

text: D7 - KO1


E-MTAB-12638:D7 - KO2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-12638:D7 - KO2

text: ArrayExpress

text: germinal center B cell

text: house mouse

text: adult

text: HellsF/F Mb1Cre+

text: 291 - J7-6

characteristic: individual

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spleen

text: Mb1 Cre-driven knockout of Hells

characteristic: phenotype

text: Mus musculus

text: male

characteristic: sex

text: C57BL/6J x 129Sv

characteristic: strain

text: D7 - KO2

characteristic: title


E-MTAB-12638:D7 - KO3

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12638:D7 - KO3

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: germinal center B cell

text: house mouse

characteristic: common name

text: adult

characteristic: developmental stage

text: HellsF/F Mb1Cre+

characteristic: genotype

text: 399 - J7-7

text: not applicable

text: Mus musculus

text: Mus musculus

text: spleen

text: Mb1 Cre-driven knockout of Hells

text: Mus musculus

text: female

text: C57BL/6J x 129Sv

text: D7 - KO3


E-MTAB-12638:D7 - KO4

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-12638:D7 - KO4

characteristic: age

text: ArrayExpress

text: germinal center B cell

text: house mouse

characteristic: description

text: adult

text: HellsF/F Mb1Cre+

text: 400 - J7-8

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: spleen

characteristic: organism part

text: Mb1 Cre-driven knockout of Hells

text: Mus musculus

text: female

text: C57BL/6J x 129Sv

text: D7 - KO4


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

tag: Namespace:Department of Medicine 1, Friedrich Alexander University, Erlangen-Nuremberg, Germany

text: ArrayExpress

text: house mouse

text: Protocols: After removing the medium, organoids were washed 3 x with 1ml PBS w/o Ca++/Mg++ and then collected for RNA extraction. Total RNA was isolated using the PeqGold microspin total RNA Kit following manufacturers' instructions (Peqlab/VWR). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on Illumina platforms, according to effective library concentration and data amount.

text: adult

text: Stat2 KO

text: intestinal organoid

characteristic: growth condition

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: small intestine

text: Mus musculus

text: male

characteristic: sex

characteristic: strain

characteristic: title


characteristic: ENA-LAST-UPDATE

text: Department of Medicine 1, Friedrich Alexander University, Erlangen-Nuremberg, Germany

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: adult

characteristic: developmental stage

text: wild type genotype

text: intestinal organoid

text: not applicable

text: Mus musculus

text: Mus musculus

text: small intestine

text: Mus musculus

text: male


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: age

text: ArrayExpress

text: house mouse

characteristic: description

text: adult

text: Stat2 KO

characteristic: genotype

text: intestinal organoid

text: not applicable

text: Mus musculus

text: Mus musculus

text: small intestine

text: Mus musculus

text: male


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: ArrayExpress

text: house mouse

text: adult

text: Stat1 KO Stat2 KO

text: intestinal organoid

characteristic: growth condition

text: not applicable

characteristic: isolate

text: Mus musculus

characteristic: organism

text: Mus musculus

text: small intestine

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex


characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

text: adult

text: Stat1 KO

text: intestinal organoid

text: not applicable

text: Mus musculus

text: Mus musculus

text: small intestine

text: Mus musculus

text: male

characteristic: strain

characteristic: title


characteristic: External Id

text: Shanghai Tenth People's Hospital

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

tag: Namespace:Shanghai Tenth People's Hospital

text: ArrayExpress

text: Protocols: Strains were maintained at -80˚C. Strains were streaked on YPD plates. The plates were incubated at 37˚C overnight. Then several colonies were picked up and suspended in DDW. Cell density was adjusted to 10,000 cells/ml, then 100µl of cell suspension was plated on YPD plates. The plates were incubated at 37˚C overnight. Colonies were picked up and suspended in DDW, then cell pellets were obtained by centrifuging at highest speed for 1min. We used the phenol-chloroform method to extract Genomic DNA. Cell pellets were suspended in 200µl of lysis buffer, add 100µl of acid washed glass beads, and 0,2ml phenol:chloroform:isoamyl alcohol (25:24:1). Vortex for 30min at 4˚C. Add 200µl TE pH7.5. Centrifuge for 5min at maximum speed. Transfer aqueous layer to a new 1.5ml EP tube. Add 1ml 100% ethanol. Precipitate 1h at -20˚C. Pellet DNA by centrifuging for 10min at maximum speed. Discard the supernatant. Add 400µl TE pH 7.5. Add 3ul of RNase A (10mg/ml). Incubate at 37˚C for 30min. Add 10µl of 3M NaOAc and 1ml of 100% ethanol. Mix by inversion. Precipitate 30min at -20˚C. Pellet DNA by centrifuging for 10min at maximum speed. Discard supernatant. Air-dry the pellet overnight. Resuspend in 50µl water. 1µg Genomic DNA was randomly fragmented by Covaris, followed by fragments selection by Agencourt AMPure XP-Medium kit to an average size of 200~400 bp. Selected fragments were end repaired and 3\"adenylate, then the adaptors were ligated to the end of these 3\"adenylate fragments. The products were amplified by PCR and purified by the Agencourt AMPure XP-Medium kit. The purified double stranded PCR products were heat denatured to single strand, then circularised by the splint oligo sequence. The single strand circle DNA (ssCirDNA) were formatted as the final library and qualified by QC.

text: wild type genotype

text: not applicable

text: Candida parapsilosis

text: Candida parapsilosis

text: whole organism

text: Candida parapsilosis


characteristic: ENA-FIRST-PUBLIC

text: Shanghai Tenth People's Hospital

text: public

characteristic: Secondary Id

text: NCBI

text: Protocols: Strains were maintained at -80˚C. Strains were streaked on YPD plates. The plates were incubated at 37˚C overnight. Then several colonies were picked up and suspended in DDW. Cell density was adjusted to 10,000 cells/ml, then 100µl of cell suspension was plated on YPD plates. The plates were incubated at 37˚C overnight. Colonies were picked up and suspended in DDW, then cell pellets were obtained by centrifuging at highest speed for 1min. We used the phenol-chloroform method to extract Genomic DNA. Cell pellets were suspended in 200µl of lysis buffer, add 100µl of acid washed glass beads, and 0,2ml phenol:chloroform:isoamyl alcohol (25:24:1). Vortex for 30min at 4˚C. Add 200µl TE pH7.5. Centrifuge for 5min at maximum speed. Transfer aqueous layer to a new 1.5ml EP tube. Add 1ml 100% ethanol. Precipitate 1h at -20˚C. Pellet DNA by centrifuging for 10min at maximum speed. Discard the supernatant. Add 400µl TE pH 7.5. Add 3ul of RNase A (10mg/ml). Incubate at 37˚C for 30min. Add 10µl of 3M NaOAc and 1ml of 100% ethanol. Mix by inversion. Precipitate 30min at -20˚C. Pellet DNA by centrifuging for 10min at maximum speed. Discard supernatant. Air-dry the pellet overnight. Resuspend in 50µl water. 1µg Genomic DNA was randomly fragmented by Covaris, followed by fragments selection by Agencourt AMPure XP-Medium kit to an average size of 200~400 bp. Selected fragments were end repaired and 3"adenylate, then the adaptors were ligated to the end of these 3"adenylate fragments. The products were amplified by PCR and purified by the Agencourt AMPure XP-Medium kit. The purified double stranded PCR products were heat denatured to single strand, then circularised by the splint oligo sequence. The single strand circle DNA (ssCirDNA) were formatted as the final library and qualified by QC.

characteristic: description

text: wild type genotype

characteristic: genotype

text: not applicable

text: Candida parapsilosis

text: Candida parapsilosis

text: whole organism

text: Candida parapsilosis

text: Candida parapsilosis


text: Shanghai Tenth People's Hospital

text: public

text: ArrayExpress

text: NCBI

text: wild type genotype

text: not applicable

characteristic: isolate

text: Candida parapsilosis

characteristic: organism

text: whole organism

characteristic: organism part

characteristic: sample name

text: Candida parapsilosis

text: Candida parapsilosis


characteristic: ENA-LAST-UPDATE

text: Shanghai Tenth People's Hospital

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: NCBI

text: wild type genotype

text: not applicable

text: Candida parapsilosis

text: whole organism

text: Candida parapsilosis

text: Candida parapsilosis


characteristic: ENA-CHECKLIST

text: Shanghai Tenth People's Hospital

text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: Protocols: Cells were grown on YPD plates at 30C for 72h. Colonies with similar sizes were randomly chosen and obtained by centrifuging at maximum speed for 1min. the pellets were fast frozen in liquid nitrogen. RNA was extracted using the TRIzol method following the manufacturer's protocol. Cell pellets were transferred from liquid nitrogen into the 2ml tub containing 1.5ml Trizol reagent. The mix was shared for 3min, and kept for 5in at room temperature, then centrifuged at 10,000xg for 5min at 4˚C. The supernatant was added 200µl of chloroform/isoamyl alcohol (24:1) with 1ml lysis reagents. After centrifuged at 10,000g for 10min at 4˚C, the supernatant was transferred into another new tube with equal volume of isopropanol and put in the refrigerator at -20˚C for 1h. After centrifuged at 13600xg for 20min at 4˚C, the supernatant was precipitated by 1ml of 75% ethanol and let dry for 3-5min. The RNA pellet was dissolved with 30-100µl of DEPC water. The concentration of the extracted RNA was determined using a Nanodrop system (NanoDrop, Madison, USA), and the integrity of the RNA was examined by the RNA integrity number (RIN) using an Agilent 2100 bioanalyzer (Agilent, Santa Clara, USA) Oligo(dT)-attached magnetic beads were used to purified mRNA. Purified mRNA was fragmented into small pieces with fragment buffer at appropriate temperature. Then First-strand cDNA was generated using random hexamer-primed reverse transcription, followed by a second-strand cDNA synthesis. afterwards, A-Tailing Mix and RNA Index Adapters were added by incubating to end repair. The cDNA fragments obtained from previous step were amplified by PCR, and products were purified by Ampure XP Beads, then dissolved in EB solution. The product was validated on the Agilent Technologies 2100 bioanalyzer for quality control. The double stranded PCR products from previous step were heated denatured and circularized by the splint oligo sequence to get the final library. The single strand circle DNA (ssCir DNA) was formatted as the final library.

text: wild type genotype

text: YPD plate

characteristic: growth condition

text: not applicable

text: Cryptococcus neoformans var. grubii H99

text: Cryptococcus neoformans var. grubii H99

text: whole organism

text: Cryptococcus neoformans var. grubii H99

characteristic: strain


characteristic: External Id

text: institut de ciencies del mar

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

tag: Namespace:institut de ciencies del mar

text: Marine biome (ENVO_00000447)

characteristic: broad-scale environmental context

characteristic: collection date

text: Manipulation experiments of surface seawater with six different treatments, which consisted on reducing cumulative growth rates controlling factors: Control (CT): incubation of untreated seawater, predator reduced (PR): seawater prefiltered through 1-um filter to remove most predators, Both (CT and PR) had two light cycles: natural light/dark cycle (CL and PL), and darkness (CD and PD), respectively, A diluted treatment (DL): whole seawater diluted 1:4 with 0,2-um filtered seawater to reduce both predation and competition for nutrient and organic carbon resources exposed to natural light/dark cycles, A virus-reduced treatment (VL): whole seawater diluted 1:4 with seawater filtered through a 30-kDa VivaFlow (tangential flow filtration) cartridge to reduce predation, viruses and resource competition, also exposed to natural light/dark cycles, The experiments were performed in 9L Nalgene bottles per triplicate, which were incubated in 200L baths with circulating water to maintain temperature close to in situ conditions during 36/48 hours (until cell abundance reached the stationary phase) and sampled every 12 hours approximately,

text: saline water

characteristic: environmental medium

text: CL

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Mediterranean Sea biome (ENVO:01000047)

characteristic: local environmental context

text: aquatic metagenome

text: REMEI

characteristic: project name

text: aquatic metagenome

characteristic: source material identifiers

text: Manipulation experiment prokaryotic gross growth rates

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: institut de ciencies del mar

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Marine biome (ENVO_00000447)

characteristic: description

text: saline water

text: PL

text: Mediterranean Sea

text: Mediterranean Sea biome (ENVO:01000047)

text: aquatic metagenome

characteristic: organism

text: REMEI

text: aquatic metagenome


characteristic: External Id

text: institut de ciencies del mar

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Marine biome (ENVO_00000447)

characteristic: broad-scale environmental context

characteristic: collection date

text: saline water

characteristic: environmental medium

text: DL

text: Mediterranean Sea

text: Mediterranean Sea biome (ENVO:01000047)

text: aquatic metagenome

text: REMEI

text: aquatic metagenome


text: institut de ciencies del mar

text: public

text: Marine biome (ENVO_00000447)

text: saline water

text: VL

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Mediterranean Sea biome (ENVO:01000047)

characteristic: local environmental context

text: aquatic metagenome

text: REMEI

characteristic: project name

text: aquatic metagenome

characteristic: source material identifiers


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: institut de ciencies del mar

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Marine biome (ENVO_00000447)

text: saline water

text: CD

text: Mediterranean Sea

text: Mediterranean Sea biome (ENVO:01000047)

text: aquatic metagenome

text: REMEI

text: aquatic metagenome

characteristic: title


characteristic: External Id

text: institut de ciencies del mar

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Marine biome (ENVO_00000447)

characteristic: broad-scale environmental context

characteristic: description

text: saline water

text: PD

text: Mediterranean Sea

text: Mediterranean Sea biome (ENVO:01000047)

text: aquatic metagenome

characteristic: organism

text: REMEI

text: aquatic metagenome


text: institut de ciencies del mar

text: public

text: Marine biome (ENVO_00000447)

characteristic: collection date

text: saline water

characteristic: environmental medium

text: in situ

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Mediterranean Sea biome (ENVO:01000047)

characteristic: local environmental context

text: aquatic metagenome

text: REMEI

text: aquatic metagenome

text: BL_170220Exp, REMEI-W


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: institut de ciencies del mar

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Marine biome (ENVO_00000447)

text: saline water

text: in situ

text: Mediterranean Sea

text: Mediterranean Sea biome (ENVO:01000047)

text: aquatic metagenome

text: REMEI

characteristic: project name

text: aquatic metagenome

text: BL_170425Exp REMEI-SP

characteristic: source material identifiers


characteristic: External Id

text: institut de ciencies del mar

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: Marine biome (ENVO_00000447)

characteristic: broad-scale environmental context

text: saline water

text: in situ

text: Mediterranean Sea

text: Mediterranean Sea biome (ENVO:01000047)

text: aquatic metagenome

text: REMEI

text: aquatic metagenome

text: BL_170704Exp REMEI-SU

characteristic: title


text: institut de ciencies del mar

text: public

text: Marine biome (ENVO_00000447)

characteristic: collection date

characteristic: description

text: saline water

characteristic: environmental medium

text: in situ

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Mediterranean Sea biome (ENVO:01000047)

text: aquatic metagenome

characteristic: organism

text: REMEI

text: aquatic metagenome

text: BL_171106EXP REMEI-Fall


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Generation

text: INRA (INRA Jouy en Josas)

text: public

characteristic: INSDC status

characteristic: Line

characteristic: SRA accession

tag: Namespace:INRA (INRA Jouy en Josas)

text: digestive tract environment [ENVO:01001033]

text: Pig gut metagenome

text: fecal material [ENVO:00002003]

text: France

characteristic: geographic location (longitude)

unit: days

characteristic: host age

text: Pig

characteristic: host common name

text: Sus scrofa

characteristic: host scientific name

characteristic: host subject id

text: Large white

characteristic: host subspecific genetic lineage

text: lower digestive tract [UBERON:0004907]

characteristic: local environmental context

text: pig gut metagenome

text: Enterotypig

text: pig gut metagenome


characteristic: External Id

text: INRA (INRA Jouy en Josas)

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: HRT

characteristic: Submitter Id

characteristic: broad-scale environmental context

characteristic: collection date

text: Pig gut metagenome

characteristic: description

text: fecal material [ENVO:00002003]

characteristic: environmental medium

text: France

characteristic: geographic location (country and/or sea)

text: Pig

text: Sus scrofa

text: Large white

text: lower digestive tract [UBERON:0004907]

text: pig gut metagenome

text: Enterotypig

characteristic: project name

text: pig gut metagenome

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Generation

text: INRA (INRA Jouy en Josas)

text: public

characteristic: INSDC status

text: HPM

characteristic: Line

characteristic: SRA accession

text: Pig gut metagenome

text: fecal material [ENVO:00002003]

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: Pig

characteristic: host common name

text: Sus scrofa

text: Large white

text: lower digestive tract [UBERON:0004907]

text: pig gut metagenome

characteristic: organism

text: Enterotypig

text: pig gut metagenome


characteristic: External Id

text: University of Edinburgh

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

tag: Namespace:University of Edinburgh

characteristic: Submitter Id

text: Atlantic white-sided dolphin

characteristic: common name

text: tissue sample

characteristic: description

text: not collected

text: Lagenorhynchus acutus

text: Lagenorhynchus acutus

text: M

characteristic: sex

text: muscle

text: Atlantic white-sided dolphin


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: University of Edinburgh

text: public

text: Atlantic white-sided dolphin

text: tissue sample

text: Scotland

characteristic: geographic location (region and locality)

text: Lagenorhynchus acutus

text: Lagenorhynchus acutus

text: M

text: muscle

text: Atlantic white-sided dolphin

characteristic: title


text: University of Edinburgh

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Atlantic white-sided dolphin

text: tissue sample

text: Scotland

text: Lagenorhynchus acutus

text: Lagenorhynchus acutus

text: F

text: muscle

text: Atlantic white-sided dolphin


text: University of Edinburgh

text: public

text: Atlantic white-sided dolphin

text: tissue sample

text: Scotland

text: Lagenorhynchus acutus

text: Lagenorhynchus acutus

text: M

text: liver

text: Atlantic white-sided dolphin


text: University of Edinburgh

text: public

text: Atlantic white-sided dolphin

text: tissue sample

text: Scotland

text: Lagenorhynchus acutus

text: Lagenorhynchus acutus

text: F

text: liver

text: Atlantic white-sided dolphin


text: University of Edinburgh

text: public

text: Atlantic white-sided dolphin

text: tissue sample

text: Faroes

text: not collected

text: Lagenorhynchus acutus

text: Lagenorhynchus acutus

text: F

text: skin

text: Atlantic white-sided dolphin


text: University of Edinburgh

text: public

text: Atlantic white-sided dolphin

text: tissue sample

text: Faroes

text: not collected

text: Lagenorhynchus acutus

text: Lagenorhynchus acutus

text: M

text: skin

text: Atlantic white-sided dolphin


text: University of Edinburgh

text: public

text: Atlantic white-sided dolphin

text: tissue sample

text: Ireland

text: Lagenorhynchus acutus

text: Lagenorhynchus acutus

text: M

text: skin

text: Atlantic white-sided dolphin


text: University of Edinburgh

text: public

text: Atlantic white-sided dolphin

text: tissue sample

text: Ireland

text: Lagenorhynchus acutus

text: Lagenorhynchus acutus

text: F

text: skin

text: Atlantic white-sided dolphin


text: University of Edinburgh

text: public

text: Atlantic white-sided dolphin

text: tissue sample

text: Ireland

text: not collected

text: Lagenorhynchus acutus

text: Lagenorhynchus acutus

text: M

text: skin

text: Atlantic white-sided dolphin


text: University of Edinburgh

text: public

text: not collected

text: Atlantic white-sided dolphin

text: tissue sample

text: Ireland

text: not collected

text: Lagenorhynchus acutus

text: Lagenorhynchus acutus

text: M

text: skin

text: Atlantic white-sided dolphin


text: University of Edinburgh

text: public

text: Atlantic white-sided dolphin

text: tissue sample

text: Ireland

text: not collected

text: Lagenorhynchus acutus

text: Lagenorhynchus acutus

text: F

text: skin

text: Atlantic white-sided dolphin


text: University of Edinburgh

text: public

text: Atlantic white-sided dolphin

text: tissue sample

text: France

text: not collected

text: Lagenorhynchus acutus

text: Lagenorhynchus acutus

text: F

text: muscle

text: Atlantic white-sided dolphin


text: University of Edinburgh

text: public

text: Atlantic white-sided dolphin

text: tissue sample

text: Scotland

text: not collected

text: Lagenorhynchus acutus

text: Lagenorhynchus acutus

text: M

text: skin

text: Atlantic white-sided dolphin


text: University of Edinburgh

text: public

text: Atlantic white-sided dolphin

text: tissue sample

text: Scotland

text: not collected

text: Lagenorhynchus acutus

text: Lagenorhynchus acutus

text: F

text: skin

text: Atlantic white-sided dolphin


text: University of Edinburgh

text: public

text: Atlantic white-sided dolphin

text: tissue sample

text: Iceland

text: Lagenorhynchus acutus

text: Lagenorhynchus acutus

text: M

text: muscle

text: Atlantic white-sided dolphin


characteristic: Generation

text: INRA (INRA Jouy en Josas)

text: public

characteristic: Line

characteristic: broad-scale environmental context

characteristic: collection date

text: Pig gut metagenome

text: fecal material [ENVO:00002003]

characteristic: environmental medium

text: France

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: Pig

characteristic: host common name

text: Sus scrofa

characteristic: host scientific name

characteristic: host subject id

text: Large white

characteristic: host subspecific genetic lineage

text: lower digestive tract [UBERON:0004907]

characteristic: local environmental context

text: pig gut metagenome

text: Enterotypig

characteristic: project name

text: pig gut metagenome


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: THE JACKSON LABORATORY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Instrument

characteristic: Instrument

tag: Namespace:THE JACKSON LABORATORY

characteristic: Submitter Id

text: human

characteristic: common name

text: GM18534_CTGGTCAT-ACGAGAAC_HF35FDSXY_L001_001.R1.fastq.gz

text: USA

text: Restriction Digest

text: GENOMIC

text: WGS

text: Homo sapiens

characteristic: organism

text: Homo sapiens

characteristic: title


text: THE JACKSON LABORATORY

text: public

text: Instrument

characteristic: SRA accession

characteristic: collection date

text: human

text: GM18534_CTGGTCAT-ACGAGAAC_HF35FDSXY_L001_001.R2.fastq.gz

text: USA

characteristic: geographic location (country and/or sea)

text: Restriction Digest

text: GENOMIC

text: WGS

text: Homo sapiens

text: Homo sapiens


text: SOUTH CHINA NORMAL UNIVERSITY

text: public

tag: Namespace:SOUTH CHINA NORMAL UNIVERSITY

characteristic: description

text: uncultured bacterium

text: uncultured bacterium

text: Five soil samples.


characteristic: ENA-CHECKLIST

text: Shanghai Tenth People's Hospital

text: public

text: ArrayExpress

characteristic: broker name

text: Protocols: Cells were grown in YPD broth at 30˚C from OD600=0.01 till OD600=1.0. Cells were treated with 1.5 µg/ml amphotericin B, or DMSO (control) for 3h. Pellets of 5ml cell culture were obtained by centrifuging at maximum speed for 1min. the pellets were fast frozen in liquid nitrogen. RNA was extracted using the TRIzol method following the manufacturer's protocol. Cell pellets were transferred from liquid nitrogen into the 2ml tub containing 1.5ml Trizol reagent. The mix was shared for 3min, and kept for 5in at room temperature, then centrifuged at 10,000xg for 5min at 4˚C. The supernatant was added 200µl of chloroform/isoamyl alcohol (24:1) with 1ml lysis reagents. After centrifuged at 10,000g for 10min at 4˚C, the supernatant was transferred into another new tube with equal volume of isopropanol and put in the refrigerator at -20˚C for 1h. After centrifuged at 13600xg for 20min at 4˚C, the supernatant was precipitated by 1ml of 75% ethanol and let dry for 3-5min. The RNA pellet was dissolved with 30-100µl of DEPC water. The concentration of the extracted RNA was determined using a Nanodrop system (NanoDrop, Madison, USA), and the integrity of the RNA was examined by the RNA integrity number (RIN) using an Agilent 2100 bioanalyzer (Agilent, Santa Clara, USA) buffer at appropriate temperature. Then First-strand cDNA was generated using random hexamer-primed reverse transcription, followed by a second-strand cDNA synthesis. afterwards, A-Tailing Mix and RNA Index Adapters were added by incubating to end repair. The cDNA fragments obtained from previous step were amplified by PCR, and products were purified by Ampure XP Beads, then dissolved in EB solution. The product was validated on the Agilent Technologies 2100 bioanalyzer for quality control. The double stranded PCR products from previous step were heated denatured and circularized by the splint oligo sequence to get the final library. The single strand circle DNA (ssCir DNA) was formatted as the final library.

text: log phase

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: not applicable

characteristic: isolate

text: Cryptococcus neoformans var. grubii H99

text: Cryptococcus neoformans var. grubii H99

text: whole organism

characteristic: organism part

text: Cryptococcus neoformans var. grubii H99

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORWEGIAN UNIVERSITY OF LIFE SCIENCES

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

tag: Namespace:NORWEGIAN UNIVERSITY OF LIFE SCIENCES

text: Atlantic salmon

text: Week 1, Freshwater, 24h light, tank 1, fish 2, fresh liver tissue

text: Salmo salar

text: Salmo salar


text: NORWEGIAN UNIVERSITY OF LIFE SCIENCES

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Atlantic salmon

characteristic: common name

text: Week 1, Freshwater, 24h light, tank 1, fish 4,fresh liver tissue

text: Salmo salar

characteristic: organism

text: Salmo salar

characteristic: title


characteristic: ENA-CHECKLIST

text: NORWEGIAN UNIVERSITY OF LIFE SCIENCES

text: public

text: Atlantic salmon

text: Week 1, Freshwater, 24h light, tank 2, fish 2, fresh liver tissue

characteristic: description

text: Salmo salar

text: Salmo salar


text: NORWEGIAN UNIVERSITY OF LIFE SCIENCES

text: public

text: Atlantic salmon

text: Week 1, Freshwater, 24h light, tank 2, fish 4,fresh liver tissue

text: Salmo salar

text: Salmo salar


text: NORWEGIAN UNIVERSITY OF LIFE SCIENCES

text: public

text: Atlantic salmon

text: Week 10, Freshwater, 8h light, tank 1, fish 2, fresh liver tissue

text: Salmo salar

text: Salmo salar


text: NORWEGIAN UNIVERSITY OF LIFE SCIENCES

text: public

text: Atlantic salmon

text: Week 10, Freshwater, 8h light, tank 1, fish 4,fresh liver tissue

text: Salmo salar

text: Salmo salar


text: NORWEGIAN UNIVERSITY OF LIFE SCIENCES

text: public

text: Atlantic salmon

text: Week 10, Freshwater, 8h light, tank 2, fish 2,fresh liver tissue

text: Salmo salar

text: Salmo salar


text: NORWEGIAN UNIVERSITY OF LIFE SCIENCES

text: public

text: Atlantic salmon

text: Week 10, Freshwater, 8h light, tank 2, fish 4,fresh liver tissue

text: Salmo salar

text: Salmo salar


text: NORWEGIAN UNIVERSITY OF LIFE SCIENCES

text: public

text: Atlantic salmon

text: Week 10 control, Freshwater, 24h light, tank 3, fish 2,fresh liver tissue

text: Salmo salar

text: Salmo salar


text: NORWEGIAN UNIVERSITY OF LIFE SCIENCES

text: public

text: Atlantic salmon

text: Week 10 control, Freshwater, 24h light, tank 3, fish 4,fresh liver tissue

text: Salmo salar

text: Salmo salar


text: NORWEGIAN UNIVERSITY OF LIFE SCIENCES

text: public

text: Atlantic salmon

text: Week 10 control, Freshwater, 24h light, tank 4, fish 2,fresh liver tissue

text: Salmo salar

text: Salmo salar


text: NORWEGIAN UNIVERSITY OF LIFE SCIENCES

text: public

text: Atlantic salmon

text: Week 10 control, Freshwater, 24h light, tank 4, fish 4,fresh liver tissue

text: Salmo salar

text: Salmo salar


text: NORWEGIAN UNIVERSITY OF LIFE SCIENCES

text: public

text: Atlantic salmon

text: Week 19, Freshwater, 24h light, tank 1, fish 2, fresh liver tissue

text: Salmo salar

text: Salmo salar


text: NORWEGIAN UNIVERSITY OF LIFE SCIENCES

text: public

text: Atlantic salmon

text: Week 19, Freshwater, 24h light, tank 1, fish 4,fresh liver tissue

text: Salmo salar

text: Salmo salar


text: NORWEGIAN UNIVERSITY OF LIFE SCIENCES

text: public

text: Atlantic salmon

text: Week 19, Freshwater, 24h light, tank 2, fish 2,fresh liver tissue

text: Salmo salar

text: Salmo salar


text: NORWEGIAN UNIVERSITY OF LIFE SCIENCES

text: public

text: Atlantic salmon

text: Week 19, Freshwater, 24h light, tank 2, fish 24,fresh liver tissue

text: Salmo salar

text: Salmo salar


text: NORWEGIAN UNIVERSITY OF LIFE SCIENCES

text: public

text: Atlantic salmon

text: Week 19 control, Freshwater, 24h light, tank 3, fish 2,fresh liver tissue

text: Salmo salar

text: Salmo salar


text: NORWEGIAN UNIVERSITY OF LIFE SCIENCES

text: public

text: Atlantic salmon

text: Week 19 control, Freshwater, 24h light, tank 3, fish 4,fresh liver tissue

text: Salmo salar

text: Salmo salar


text: NORWEGIAN UNIVERSITY OF LIFE SCIENCES

text: public

text: Atlantic salmon

text: Week 19 control, Freshwater, 24h light, tank 4, fish 2,fresh liver tissue

text: Salmo salar

text: Salmo salar


text: NORWEGIAN UNIVERSITY OF LIFE SCIENCES

text: public

text: Atlantic salmon

text: Week 19 control, Freshwater, 24h light, tank 4, fish 4,fresh liver tissue

text: Salmo salar

text: Salmo salar


text: NORWEGIAN UNIVERSITY OF LIFE SCIENCES

text: public

text: Atlantic salmon

text: Week 25, Saltwater, 24h light, tank 1, fish 2, fresh liver tissue

text: Salmo salar

text: Salmo salar


text: NORWEGIAN UNIVERSITY OF LIFE SCIENCES

text: public

text: Atlantic salmon

text: Week 25, Saltwater, 24h light, tank 2, fish 2, fresh liver tissue

text: Salmo salar

text: Salmo salar


text: NORWEGIAN UNIVERSITY OF LIFE SCIENCES

text: public

text: Atlantic salmon

text: Week 25, Saltwater, 24h light, tank 2, fish 4, fresh liver tissue

text: Salmo salar

text: Salmo salar


text: NORWEGIAN UNIVERSITY OF LIFE SCIENCES

text: public

text: Atlantic salmon

text: Week 25 control, Saltwater, 24h light, tank 3, fish 2, fresh liver tissue

text: Salmo salar

text: Salmo salar


text: NORWEGIAN UNIVERSITY OF LIFE SCIENCES

text: public

text: Atlantic salmon

text: Week 25 control, Saltwater, 24h light, tank 3, fish 4, fresh liver tissue

text: Salmo salar

text: Salmo salar


text: NORWEGIAN UNIVERSITY OF LIFE SCIENCES

text: public

text: Atlantic salmon

text: Week 25 control, Saltwater, 24h light, tank 4, fish 2, fresh liver tissue

text: Salmo salar

text: Salmo salar


text: NORWEGIAN UNIVERSITY OF LIFE SCIENCES

text: public

text: Atlantic salmon

text: Week 25 control, Saltwater, 24h light, tank 4, fish 4, fresh liver tissue

text: Salmo salar

text: Salmo salar


text: public

tag: Namespace:UCL Great Ormond Street Institute of Child Health

unit: month

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

text: Protocols: Mice were sacrificed by cervical dislocation. The inner ear was immediately isolated from surrounding tissue and the cochlea was separated from the vestibular region, the stapedial artery removed and the cochlea snap frozen Total RNA was extracted using a modification of a published protocol (PMID 25818515) (TRI Reagent® 93289-25ML Sigma-Aldrich, DirectZol kit). RNA was eluted in 40-50 μl of nuclease-free water and analysed using the by NanoDropTM 2000 (Thermo Scientific) and Agilent Bioanalyzer platforms. LIbraries were constructed for Illumina, strand-specific RNA-seq with PolyA selection according to standard protocols by Azenta Lifesciences

text: adult

characteristic: developmental stage

text: Norrie disease

characteristic: disease

text: Ndp-KO

characteristic: genotype

characteristic: individual

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: cochlea

text: Mus musculus

text: male

characteristic: sex

text: AAV NDP gene therapy

characteristic: treatment


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCL Great Ormond Street Institute of Child Health

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: house mouse

text: adult

text: Norrie disease

text: Ndp-KO

text: not applicable

text: Mus musculus

text: Mus musculus

text: cochlea

characteristic: organism part

text: Mus musculus

text: male

characteristic: strain

text: AAV NDP gene therapy


characteristic: ENA-CHECKLIST

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: Norrie disease

text: Ndp-KO

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: cochlea

text: Mus musculus

text: male

characteristic: title

text: Control


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

characteristic: individual

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: cochlea

text: Mus musculus

text: male

characteristic: sex

text: Control

characteristic: treatment


characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:CENTER FOR BIOTECHNOLOGY, BIELEFELD UNIVERSITY

text: thale cress

text: Single-end RNA-Seq data from 6 days old seedlings of Arabidopsis thaliana Columbia-0 (Col-0, replicate 1)

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: CENTER FOR BIOTECHNOLOGY, BIELEFELD UNIVERSITY

text: public

characteristic: Submitter Id

text: thale cress

characteristic: common name

text: Single-end RNA-Seq data from 6 days old seedlings of Arabidopsis thaliana Columbia-0 (Col-0, replicate 2)

characteristic: description

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis thaliana

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: thale cress

text: Single-end RNA-Seq data from 6 days old seedlings of Arabidopsis thaliana Columbia-0 (Col-0, replicate 3)

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: public

text: thale cress

text: Single-end RNA-Seq data from 6 days old seedlings of Arabidopsis thaliana Columbia-0 (Col-0, replicate 4)

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: public

text: thale cress

text: Single-end RNA-Seq data from 6 days old seedlings of Arabidopsis thaliana Noessen-0 (No-0, replicate 1)

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: public

text: thale cress

text: Single-end RNA-Seq data from 6 days old seedlings of Arabidopsis thaliana Noessen-0 (No-0, replicate 2)

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: public

text: thale cress

text: Single-end RNA-Seq data from 6 days old seedlings of Arabidopsis thaliana Noessen-0 (No-0, replicate 3)

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: public

text: thale cress

text: Single-end RNA-Seq data from 6 days old seedlings of Arabidopsis thaliana Noessen-0 (No-0, replicate 4)

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: public

text: thale cress

text: Single-end RNA-Seq data from 6 days old seedlings of Arabidopsis thaliana f3h/fls1/ans mutant (3xODD, replicate 1)

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: public

text: thale cress

text: Single-end RNA-Seq data from 6 days old seedlings of Arabidopsis thaliana f3h/fls1/ans mutant (3xODD, replicate 2)

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: public

text: thale cress

text: Single-end RNA-Seq data from 6 days old seedlings of Arabidopsis thaliana f3h/fls1/ans mutant (3xODD, replicate 3)

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: public

text: thale cress

text: Single-end RNA-Seq data from 6 days old seedlings of Arabidopsis thaliana f3h/fls1/ans mutant (3xODD, replicate 4)

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: public

text: thale cress

text: FASTQ files of ONT long-read data from leaves of Arabidopsis thaliana f3h/fls1/ans mutant

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: public

text: thale cress

text: FAST5 files of ONT long-read data from leaves of Arabidopsis thaliana f3h/fls1/ans mutant

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: VANDERBILT UNIVERSITY

text: public

tag: Namespace:VANDERBILT UNIVERSITY

text: embryonic day 9.0 to 9.5

unit: day

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

text: Protocols: Embryos were dissected in ice-cold PBS and staged by Theiler’s criteria. Embryos of the same genotype within E9.0 to E9.5 range were pooled, incubated for 10 minutes at 37oC with Accumax (Millipore Sigma, #A7089) supplemented with DNase (Life technologies, #AM2222; 1:1,000 dilution ratio), and further disaggregated by vigorous pipetting during incubation. Passing the cell suspension through 35 µm strainer caps (Corning, #352235) led to single cells, which were washed with FACS buffer (R&D systems, #FC001) and pelleted at 4oC, 1000 rpm for 3 mins. Cells were resuspended in FACS buffer containing DNaseI (1:1,000 dilution) and stained with 7AAD (15 mins, room temperature - RT). Live cells (7AAD-negative) expressing either GFP or TdTomato fluorescence were then sorted into DMEM with 10% FBS and further centrifuged and resuspended at 600 cells/μl concentration required for sequencing step. RNA was extracted according to the manufacturer’s instructions for 10x Genomics Single Cell 3′ Library kit v3 Libraries were constructed according to the manufacturer’s instructions for 10x Genomics Single Cell 3′ Library kit v3

text: embryo

characteristic: developmental stage

text: Sox17.GFPCre heterozygous; R26.WT homozygous

characteristic: genotype

characteristic: individual

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: embryo

characteristic: organism part

text: GFP-positive

characteristic: phenotype

text: Mus musculus

text: mixed

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VANDERBILT UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: embryonic day 9.0 to 9.5

text: ArrayExpress

text: house mouse

text: embryo

text: Sox17.GFPCre heterozygous; R26.LSLTdTomato heterozygous

text: not applicable

text: Mus musculus

text: Mus musculus

text: embryo

text: TdTomato-positive

text: Mus musculus

text: mixed

characteristic: sex

text: CD1 x C57Bl/6J


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Guy's and St Thomas' Trust

text: public

tag: Namespace:Guy's and St Thomas' Trust

characteristic: Submitter Id

text: Negative process control for metagenomic libraries

characteristic: description

text: metagenome

characteristic: organism

text: metagenome

text: Negative process control

characteristic: title


text: Guy's and St Thomas' Trust

text: public

text: Human lung metagenome using host-depletion pipeline after 2 hrs of nanopore sequencing

text: lung metagenome

text: lung metagenome

text: Sample 2hrs


text: Guy's and St Thomas' Trust

text: public

text: Positive control for metagenomic libraries

text: metagenome

text: metagenome

text: Positive control


text: Guy's and St Thomas' Trust

text: public

text: Negative process control with spiked organism (Jonesia denitrificans) for metagenomic libraries

text: metagenome

text: metagenome

text: Spiked control


text: public

tag: Namespace:WAGENINGEN UNIVERSITY, LABORATORY OF MICROBIOLOGY

text: acid mine drainage

characteristic: broad-scale environmental context

characteristic: collection date

unit: m

characteristic: depth

text: Rio Tintillo water below FMF

text: acid mine drainage

characteristic: environmental medium

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: acidic stream

characteristic: local environmental context

text: mine drainage metagenome

text: AMD Floating Mineral Films

characteristic: project name

text: mine drainage metagenome


tag: attribute

characteristic: ENA-CHECKLIST

characteristic: External Id

text: WAGENINGEN UNIVERSITY, LABORATORY OF MICROBIOLOGY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: acid mine drainage

text: Rio Tintillo water below FMF

text: acid mine drainage

text: Spain

text: acidic stream

text: mine drainage metagenome

text: AMD Floating Mineral Films

text: mine drainage metagenome


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: THE JACKSON LABORATORY

text: public

text: Instrument

characteristic: Instrument

characteristic: Submitter Id

text: human

characteristic: common name

text: NA18989_GT21-11682_ACTAAGAT-CCGCGGTT_S25_L002_R2_001.fastq.gz

text: USA

text: PolyA

text: TRANSCRIPTOMIC

text: RNASeq

text: Homo sapiens

characteristic: organism

text: Homo sapiens

characteristic: title


text: THE JACKSON LABORATORY

text: public

text: Instrument

characteristic: collection date

text: human

text: HG02554_GT21-11705_GTTCCAAT-GCAGAATT_S44_L004_R1_001.fastq.gz

text: USA

characteristic: geographic location (country and/or sea)

text: PolyA

text: TRANSCRIPTOMIC

text: RNASeq

text: Homo sapiens

text: Homo sapiens


text: THE JACKSON LABORATORY

text: public

text: Instrument

text: human

text: NA19317_GT21-11685_TCCAACGC-AAGTCCAA_S24_L002_R2_001.fastq.gz

text: USA

text: PolyA

text: TRANSCRIPTOMIC

text: RNASeq

text: Homo sapiens

text: Homo sapiens


text: THE JACKSON LABORATORY

text: public

text: Instrument

text: human

text: NA19331_GT21-11683_GTCGGAGC-TTATAACC_S19_L002_R1_001.fastq.gz

text: USA

text: PolyA

text: TRANSCRIPTOMIC

text: RNASeq

text: Homo sapiens

text: Homo sapiens


text: THE JACKSON LABORATORY

text: public

text: Instrument

text: human

text: HG02953_GT21-11706_ACCTTGGC-ATGAGGCC_S39_L004_R2_001.fastq.gz

text: USA

text: PolyA

text: TRANSCRIPTOMIC

text: RNASeq

text: Homo sapiens

text: Homo sapiens


text: THE JACKSON LABORATORY

text: public

text: Instrument

text: human

text: NA19347_GT21-11686_CCGTGAAG-ATCCACTG_S27_L002_R2_001.fastq.gz

text: USA

text: PolyA

text: TRANSCRIPTOMIC

text: RNASeq

text: Homo sapiens

text: Homo sapiens


text: THE JACKSON LABORATORY

text: public

text: Instrument

text: human

text: HG03248_GT21-11687_TTACAGGA-GCTTGTCA_S23_L002_R2_001.fastq.gz

text: USA

text: PolyA

text: TRANSCRIPTOMIC

text: RNASeq

text: Homo sapiens

text: Homo sapiens


text: THE JACKSON LABORATORY

text: public

text: Instrument

text: human

text: HG02554_GT21-11705_GTTCCAAT-GCAGAATT_S44_L004_R2_001.fastq.gz

text: USA

text: PolyA

text: TRANSCRIPTOMIC

text: RNASeq

text: Homo sapiens

text: Homo sapiens


text: THE JACKSON LABORATORY

text: public

text: Instrument

text: human

text: NA19317_GT21-11685_TCCAACGC-AAGTCCAA_S24_L002_R1_001.fastq.gz

text: USA

text: PolyA

text: TRANSCRIPTOMIC

text: RNASeq

text: Homo sapiens

text: Homo sapiens


text: THE JACKSON LABORATORY

text: public

text: Instrument

text: human

text: NA19384_GT21-11689_AATGCCTC-TGGATCGA_S20_L002_R1_001.fastq.gz

text: USA

text: PolyA

text: TRANSCRIPTOMIC

text: RNASeq

text: Homo sapiens

text: Homo sapiens


text: THE JACKSON LABORATORY

text: public

text: Instrument

text: human

text: NA19347_GT21-11686_CCGTGAAG-ATCCACTG_S27_L002_R1_001.fastq.gz

text: USA

text: PolyA

text: TRANSCRIPTOMIC

text: RNASeq

text: Homo sapiens

text: Homo sapiens


text: THE JACKSON LABORATORY

text: public

text: Instrument

text: human

text: NA19331_GT21-11683_GTCGGAGC-TTATAACC_S19_L002_R2_001.fastq.gz

text: USA

text: PolyA

text: TRANSCRIPTOMIC

text: RNASeq

text: Homo sapiens

text: Homo sapiens


text: THE JACKSON LABORATORY

text: public

text: Instrument

text: human

text: HG01457_GT21-11688_GGCATTCT-CAAGCTAG_S21_L002_R2_001.fastq.gz

text: USA

text: PolyA

text: TRANSCRIPTOMIC

text: RNASeq

text: Homo sapiens

text: Homo sapiens


text: THE JACKSON LABORATORY

text: public

text: Instrument

text: human

text: HG02666_GT21-11684_CTTGGTAT-GGACTTGG_S26_L002_R1_001.fastq.gz

text: USA

text: PolyA

text: TRANSCRIPTOMIC

text: RNASeq

text: Homo sapiens

text: Homo sapiens


text: THE JACKSON LABORATORY

text: public

text: Instrument

text: human

text: HG03248_GT21-11687_TTACAGGA-GCTTGTCA_S23_L002_R1_001.fastq.gz

text: USA

text: PolyA

text: TRANSCRIPTOMIC

text: RNASeq

text: Homo sapiens

text: Homo sapiens


text: THE JACKSON LABORATORY

text: public

text: Instrument

text: human

text: HG01457_GT21-11688_GGCATTCT-CAAGCTAG_S21_L002_R1_001.fastq.gz

text: USA

text: PolyA

text: TRANSCRIPTOMIC

text: RNASeq

text: Homo sapiens

text: Homo sapiens


text: THE JACKSON LABORATORY

text: public

text: Instrument

text: human

text: NA18989_GT21-11682_ACTAAGAT-CCGCGGTT_S25_L002_R1_001.fastq.gz

text: USA

text: PolyA

text: TRANSCRIPTOMIC

text: RNASeq

text: Homo sapiens

text: Homo sapiens


text: THE JACKSON LABORATORY

text: public

text: Instrument

text: human

text: NA19384_GT21-11689_AATGCCTC-TGGATCGA_S20_L002_R2_001.fastq.gz

text: USA

text: PolyA

text: TRANSCRIPTOMIC

text: RNASeq

text: Homo sapiens

text: Homo sapiens


text: THE JACKSON LABORATORY

text: public

text: Instrument

text: human

text: HG02953_GT21-11706_ACCTTGGC-ATGAGGCC_S39_L004_R1_001.fastq.gz

text: USA

text: PolyA

text: TRANSCRIPTOMIC

text: RNASeq

text: Homo sapiens

text: Homo sapiens


text: THE JACKSON LABORATORY

text: public

text: Instrument

text: human

text: HG02666_GT21-11684_CTTGGTAT-GGACTTGG_S26_L002_R2_001.fastq.gz

text: USA

text: PolyA

text: TRANSCRIPTOMIC

text: RNASeq

text: Homo sapiens

text: Homo sapiens


text: public

tag: Namespace:MONASH INSTITUTE OF MEDICAL RESEARCH - MONASH UNIVERSITY

text: Bacterial isolate from the human gastrointestinal tract

characteristic: description

text: Escherichia coli

text: Escherichia coli


characteristic: ENA-CHECKLIST

text: MONASH INSTITUTE OF MEDICAL RESEARCH - MONASH UNIVERSITY

text: public

text: Enterococcus durans

text: Enterococcus durans


characteristic: External Id

text: public

text: Proteus mirabilis

text: Proteus mirabilis


characteristic: INSDC center name

text: public

text: Streptococcus equinus

text: Streptococcus equinus


characteristic: INSDC first public

text: public

text: Bacteroides cellulosilyticus

text: Bacteroides cellulosilyticus


characteristic: INSDC last update

text: public

text: Bacteroides uniformis

text: Bacteroides uniformis


text: public

characteristic: INSDC status

text: Phocaeicola dorei CL03T12C01

text: Phocaeicola dorei CL03T12C01


text: public

characteristic: SRA accession

text: Bacteroides thetaiotaomicron

text: Bacteroides thetaiotaomicron


characteristic: ENA-FIRST-PUBLIC

text: public

text: Klebsiella oxytoca G54

text: Klebsiella oxytoca G54


characteristic: ENA-LAST-UPDATE

text: public

text: Pseudomonas aeruginosa P47

text: Pseudomonas aeruginosa P47


text: public

text: Streptococcus anginosus

text: Streptococcus anginosus


text: public

text: Enterococcus hirae

text: Enterococcus hirae


text: public

text: Citrobacter werkmanii

text: Citrobacter werkmanii


text: public

text: Streptococcus sanguinis SK36

text: Streptococcus sanguinis SK36


text: public

text: Bacteroides xylanisolvens SD CC 1b

text: Bacteroides xylanisolvens SD CC 1b


text: public

text: Escherichia coli

text: Escherichia coli


text: public

text: Parabacteroides distasonis CL09T03C24

text: Parabacteroides distasonis CL09T03C24


text: public

text: [Eubacterium] rectale ATCC 33656

text: [Eubacterium] rectale ATCC 33656


text: public

text: Enterocloster bolteae

text: Enterocloster bolteae


text: public

text: Bacteroides fragilis

text: Bacteroides fragilis


text: public

text: [Clostridium] innocuum

text: [Clostridium] innocuum


text: public

text: Blautia obeum

text: Blautia obeum


text: public

text: Coprobacillus sp. 8_2_54BFAA

text: Coprobacillus sp. 8_2_54BFAA


text: public

text: Fournierella massiliensis

text: Fournierella massiliensis


text: public

text: Lachnospiraceae bacterium 5_1_63FAA

text: Lachnospiraceae bacterium 5_1_63FAA


text: public

text: Bacteroides sp. D22

text: Bacteroides sp. D22


text: public

text: Parabacteroides merdae CL09T00C40

text: Parabacteroides merdae CL09T00C40


text: public

text: Blautia sp. Marseille-P2398

text: Blautia sp. Marseille-P2398


text: public

text: Collinsella aerofaciens

text: Collinsella aerofaciens


text: public

text: Dorea longicatena

text: Dorea longicatena


text: public

text: Enterococcus faecalis

text: Enterococcus faecalis


text: public

text: Bifidobacterium pseudocatenulatum IPLA36007


text: public

text: Bacteroides caccae

text: Bacteroides caccae


text: public

text: Bifidobacterium adolescentis

text: Bifidobacterium adolescentis


text: public

text: Anaerobutyricum hallii

text: Anaerobutyricum hallii


text: public

text: Enterococcus sp. RIT-PI-f

text: Enterococcus sp. RIT-PI-f


text: public

text: Faecalicatena contorta

text: Faecalicatena contorta


text: public

text: Lactococcus garvieae IPLA 31405

text: Lactococcus garvieae IPLA 31405


text: public

text: Bifidobacterium breve

text: Bifidobacterium breve


text: public

text: Enterococcus faecium MRSN 4777

text: Enterococcus faecium MRSN 4777


text: public

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: Wageningen University

text: public

tag: Namespace:Wageningen University

text: roadside

characteristic: broad-scale environmental context

characteristic: depth

characteristic: elevation

text: soil

characteristic: environmental medium

text: Netherlands

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Arabidopsis thaliana root

characteristic: local environmental context

text: soil metagenome

text: A.thaliana root-associated soil Veluwe Netherlands

characteristic: project name

text: soil metagenome


characteristic: ENA-CHECKLIST

text: Wageningen University

text: public

characteristic: Submitter Id

text: grassland

characteristic: collection date

characteristic: description

text: soil

text: Netherlands

characteristic: geographic location (country and/or sea)

text: Arabidopsis thaliana root

text: soil metagenome

characteristic: organism

text: soil metagenome

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: Wageningen University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: grassland

text: MV-3(16S) and MO-2(ITS)

text: soil

text: Netherlands

text: Arabidopsis thaliana root

text: soil metagenome

text: soil metagenome

text: V81_16S and V85_ITS


characteristic: ENA-LAST-UPDATE

text: Wageningen University

text: public

text: agricultural site

characteristic: broad-scale environmental context

characteristic: depth

characteristic: elevation

text: soil

characteristic: environmental medium

text: Netherlands

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Arabidopsis thaliana root

text: soil metagenome

text: soil metagenome


Klebsiella pneumonaie isolate 1

characteristic: ENA-CHECKLIST

text: University of Brescia

text: public

text: Klebsiella pneumonaie isolate 1

tag: Namespace:University of Brescia

characteristic: Submitter Id

characteristic: collection date

text: Klebsiella pneumonaie isolate 1

characteristic: description

text: Italy

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

characteristic: isolate

text: pus

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


Klebsiella pneumonaie isolate 2

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: University of Brescia

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Klebsiella pneumonaie isolate 2

text: Klebsiella pneumonaie isolate 2

text: Italy

text: not collected

text: Homo sapiens

text: tracheal aspiration

text: Klebsiella pneumoniae

characteristic: organism

text: Klebsiella pneumoniae

characteristic: title


characteristic: ENA-LAST-UPDATE

text: CGE

text: public

characteristic: SRA accession

text: DTU-GE

characteristic: broker name

text: FVST

text: Denmark

text: Bos taurus

text: Illumina MiSeq

characteristic: instrument

text: S. Dublin

text: Cattle or beef

text: Salmonella enterica subsp. enterica

text: Salmonella enterica subsp. enterica

characteristic: strain


characteristic: ENA-CHECKLIST

text: CGE

text: public

characteristic: Submitter Id

text: DTU-GE

text: FVST

characteristic: description

text: Denmark

characteristic: geographic location (country and/or sea)

text: Bos taurus

text: Illumina MiSeq

text: S. Dublin

text: Cattle or beef

text: Salmonella enterica subsp. enterica

text: Salmonella enterica subsp. enterica

text: STNF


characteristic: ENA-FIRST-PUBLIC

text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: Leishmania infantum IsoSeq raw reads

text: Leishmania infantum JPCM5

text: Leishmania infantum JPCM5

text: Leishmania infantum IsoSeq raw reads


characteristic: External Id

text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: Leishmania donovani IsoSeq raw reads

text: Leishmania donovani donovani

text: Leishmania donovani donovani

text: Leishmania donovani IsoSeq raw reads


text: GENGS-CBMSO

characteristic: INSDC center name

text: public

text: GENGS-CBMSO

text: Leishmania braziliensis IsoSeq raw reads

text: Leishmania braziliensis MHOM/BR/75/M2904


text: GENGS-CBMSO

characteristic: INSDC first public

text: public

text: GENGS-CBMSO

text: Leishmania major IsoSeq raw reads

text: Leishmania major strain Friedlin

text: Leishmania major strain Friedlin

text: Leishmania major IsoSeq raw reads


characteristic: INSDC last update

text: public

tag: Namespace:Dipannita Ghosh, Indian Institute of Technology, New Delhi

text: IBDC

characteristic: common name

text: Uropathogen causing community acquired UTI

text: India

text: diseased

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: isolate

characteristic: isolate

text: Urine

text: Escherichia coli

text: Escherichia coli


characteristic: ENA-LAST-UPDATE

text: Dipannita Ghosh, Indian Institute of Technology, New Delhi

text: public

characteristic: INSDC status

characteristic: SRA accession

text: IBDC

text: India

text: diseased

text: Homo sapiens

text: isolate

text: Urine

text: Escherichia coli

characteristic: organism

text: Escherichia coli

characteristic: title


characteristic: ENA-CHECKLIST

text: IIT DELHI

text: public

tag: Namespace:IIT DELHI

characteristic: Submitter Id

text: IBDC

characteristic: broker name

text: India

text: diseased

text: Homo sapien

text: Urine

text: Escherichia coli

text: Escherichia coli


SAMIN0000665-ID 16

characteristic: ENA-FIRST-PUBLIC

text: IIT DELHI

text: public

text: SAMIN0000665-ID 16

text: IBDC

characteristic: description

text: India

characteristic: geographic location (country and/or sea)

text: diseased

text: Homo sapien

text: Urine

text: Escherichia coli

text: Escherichia coli

text: ID 16


SAMIN0000666-ID 17

characteristic: External Id

text: IIT DELHI

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: SAMIN0000666-ID 17

text: IBDC

text: India

text: diseased

text: Homo sapien

text: Urine

text: Escherichia coli

text: Escherichia coli

text: ID 17


SAMIN0000667-ID 18

text: IIT DELHI

characteristic: INSDC last update

text: public

text: SAMIN0000667-ID 18

text: IBDC

characteristic: common name

text: India

text: diseased

characteristic: host health state

text: Homo sapien

text: Urine

text: Escherichia coli

text: Escherichia coli

text: ID 18


SAMIN0000668-ID 19

characteristic: ENA-LAST-UPDATE

text: IIT DELHI

text: public

text: SAMIN0000668-ID 19

text: IBDC

text: India

text: diseased

text: Homo sapien

characteristic: host scientific name

characteristic: isolate

text: Urine

text: Escherichia coli

text: Escherichia coli

text: ID 19


SAMIN0000669-ID 22

text: IIT DELHI

text: public

characteristic: INSDC status

characteristic: SRA accession

text: SAMIN0000669-ID 22

text: IBDC

text: India

text: diseased

text: Homo sapien

text: Urine

text: Escherichia coli

characteristic: organism

text: Escherichia coli

text: ID 22


SAMIN0000670-ID 23

characteristic: ENA-CHECKLIST

text: IIT DELHI

text: public

text: SAMIN0000670-ID 23

characteristic: Submitter Id

text: IBDC

text: India

text: diseased

text: Homo sapien

text: Urine

text: Escherichia coli

text: Escherichia coli

text: ID 23

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: public

tag: Namespace:Istituto di Ricerche Farmacologiche \"Mario Negri\" IRCCS

characteristic: age

text: ArrayExpress

characteristic: broker name

text: KPC

text: Epcam+; CD45-PDAC cells

text: house mouse

text: Protocols: PDAC tumors were isolated, minced and processed for single cell suspension. Tissues were digested in 2 ml of Digestion Buffer composed by Collagenase IV 3% in RPMI 10% FBS + 1% P/S, DNAasi I 30U/ul, Hepes 10 mM. The cell suspension was incubated for 30 min at 37oC on a rocker. Then, the digestion was stopped adding 1 mL of RPMI 10% FBS + 1% P/S. The cells suspension was filtered through a 100 µm cell strainer and kept on ice for 4 minutes. SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian (Clontech Laboratories, Inc., A Takara Bio Company,California, USA) protocol total RNA samples (0.25–10 ng) were reverse-transcribed using random priming into double-stranded cDNA in the presence of a template switch oligo (TSO). When the reverse transcriptase reaches the 5’ end of the RNA fragment, the enzyme’s terminal transferase activity adds non-templated nucleotides to the 3’ end of the cDNA. The TSO pairs with the added non-templated nucleotide, enabling the reverse transcriptase to continue replicating to the end of the oligonucleotide. This results in a cDNA fragment that contains sequences derived from the random priming oligo and the TSO. PCR amplification using primers binding to these sequences can now be performed. The PCR adds full-length Illumina adapters, including dual barcodes for multiplexing. Ribosomal cDNA is cleaved by ZapR in the presence of the mammalian-specific R-Probes. Remaining fragments are enriched with a second round of PCR amplification using primers designed to match Illumina adapters.

characteristic: description

text: pancreatic cancer

characteristic: disease

text: KHKA-/-

characteristic: genotype

text: in vivo until neoplasm formation

characteristic: growth condition

text: not applicable

text: Mus musculus

text: Mus musculus

text: pancreas

characteristic: organism part

text: neoplasm

characteristic: sampling site

text: Mus musculus

characteristic: strain


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Istituto di Ricerche Farmacologiche \"Mario Negri\" IRCCS

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: KPC

text: Epcam+; CD45-PDAC cells

text: house mouse

characteristic: common name

text: pancreatic cancer

text: KHKA/C-/-

text: in vivo until neoplasm formation

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: pancreas

text: neoplasm

text: Mus musculus


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: KPC

text: Epcam+; CD45-PDAC cells

text: house mouse

text: pancreatic cancer

text: KHKA-/-

text: in vivo until neoplasm formation

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: pancreas

text: neoplasm

text: Mus musculus

characteristic: title


text: public

characteristic: age

text: ArrayExpress

characteristic: broker name

text: KPC

text: Epcam+; CD45-PDAC cells

text: house mouse

characteristic: description

text: pancreatic cancer

characteristic: disease

text: KHKC-/-

characteristic: genotype

text: in vivo until neoplasm formation

characteristic: growth condition

text: not applicable

text: Mus musculus

text: Mus musculus

text: pancreas

characteristic: organism part

text: neoplasm

text: Mus musculus


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: KPC

text: Epcam+; CD45-PDAC cells

text: house mouse

text: pancreatic cancer

text: wild type genotype

text: in vivo until neoplasm formation

text: not applicable

text: Mus musculus

text: Mus musculus

text: pancreas

text: neoplasm

characteristic: sampling site

text: Mus musculus

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF GLASGOW

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:UNIVERSITY OF GLASGOW

characteristic: Submitter Id

text: gut metagenome

text: gut metagenome


text: University of Lubeck

text: public

tag: Namespace:University of Lubeck

text: human

characteristic: common name

characteristic: description

text: Homo sapiens

characteristic: organism

text: Homo sapiens

characteristic: title


text: ETH Zurich

text: public

tag: Namespace:ETH Zurich

text: human

text: Homo sapiens

text: Homo sapiens


Bohus polled 1

text: public

characteristic: Secondary Id

text: Bohus polled 1

tag: Namespace:Swedish University of Agricultural Sciences

text: NCBI

characteristic: broker name

text: cattle

text: cattle

text: Bohus polled 1

text: Bos taurus

text: Bos taurus

text: Bos taurus

text: Bos taurus

text: female

characteristic: sex


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Swedish University of Agricultural Sciences

characteristic: INSDC center name

text: public

text: Bohus polled 2

text: NCBI

text: cattle

text: cattle

text: Bohus polled 2

text: Bos taurus

text: Bohus polled 2

characteristic: sample name

text: Bos taurus

text: Bos taurus

text: female


Bohus polled 3

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Bohus polled 3

text: NCBI

text: cattle

text: cattle

text: Bohus polled 3

text: Bos taurus

text: Bos taurus

text: Bos taurus

text: Bos taurus

text: male


Swedish mountain cattle 1

characteristic: ENA-FIRST-PUBLIC

text: public

text: Swedish mountain cattle 1

text: NCBI

text: cattle

text: cattle

text: Swedish mountain cattle 1

text: Bos taurus

text: Bos taurus

text: Bos taurus

text: Bos taurus

text: female


Swedish mountain cattle 2

text: public

characteristic: INSDC status

text: Swedish mountain cattle 2

text: NCBI

text: cattle

text: cattle

text: Swedish mountain cattle 2

text: Bos taurus

text: Bos taurus

text: Bos taurus

text: Bos taurus

text: female


Swedish mountain cattle 3

text: public

characteristic: SRA accession

text: Swedish mountain cattle 3

text: NCBI

text: cattle

text: cattle

text: Swedish mountain cattle 3

text: Bos taurus

text: Bos taurus

text: Bos taurus

text: Bos taurus

text: female


Swedish mountain cattle 4

text: public

text: Swedish mountain cattle 4

characteristic: Submitter Id

text: NCBI

text: cattle

text: cattle

text: Swedish mountain cattle 4

text: Bos taurus

text: Bos taurus

text: Bos taurus

text: Bos taurus

text: female


Swedish mountain cattle 5

text: public

text: Swedish mountain cattle 5

text: NCBI

text: cattle

characteristic: common name

text: cattle

text: Swedish mountain cattle 5

text: Bos taurus

text: Bos taurus

text: Bos taurus

text: Bos taurus

text: female


Swedish mountain cattle 6

text: public

text: Swedish mountain cattle 6

text: NCBI

text: cattle

text: cattle

text: Swedish mountain cattle 6

characteristic: description

text: Bos taurus

text: Bos taurus

text: Bos taurus

text: Bos taurus

text: female


Swedish mountain cattle 7

text: public

text: Swedish mountain cattle 7

text: NCBI

text: cattle

text: cattle

text: Swedish mountain cattle 7

text: Bos taurus

characteristic: organism

text: Bos taurus

text: Bos taurus

text: Bos taurus

text: female


Fj?lln?ra cattle 1

text: public

text: Fj?lln?ra cattle 1

text: NCBI

text: cattle

text: cattle

text: Fj�lln�ra cattle 1

text: Bos taurus

text: Bos taurus

text: Bos taurus

text: Bos taurus

text: female

characteristic: title


Fj?lln?ra cattle 2

text: public

characteristic: Secondary Id

text: Fj?lln?ra cattle 2

text: NCBI

text: cattle

text: cattle

text: Fj�lln�ra cattle 2

text: Bos taurus

text: Bos taurus

text: Bos taurus

text: Bos taurus

text: female


Fj?lln?ra cattle 3

text: public

text: Fj?lln?ra cattle 3

text: NCBI

characteristic: broker name

text: cattle

text: cattle

text: Fj�lln�ra cattle 3

text: Bos taurus

text: Bos taurus

text: Bos taurus

text: Bos taurus

text: female


Fj?lln?ra cattle 4

text: public

text: Fj?lln?ra cattle 4

text: NCBI

text: cattle

text: cattle

text: Fj�lln�ra cattle 4

text: Bos taurus

text: Bos taurus

text: Bos taurus

text: Bos taurus

text: male

characteristic: sex


Swedish red polled 1

characteristic: ENA-LAST-UPDATE

text: public

text: Swedish red polled 1

text: NCBI

text: cattle

text: cattle

text: Swedish red polled 1

text: Bos taurus

text: Bos taurus

text: Bos taurus

text: Bos taurus

text: female