characteristic: INSDC last update

text: public

text: ena-SAMPLE-TAB-02-12-2022-09:25:35:783-28378

characteristic: Submitter Id

text: Clinical isolate

text: Egypt

characteristic: geographic location (country and/or sea)

text: diseased

text: Homo sapiens

text: Acinetobacter baumannii 20Y0070

text: Blood

text: Acinetobacter baumannii

text: Acinetobacter baumannii


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-02-12-2022-09:25:35:784-28383

tag: Namespace:October University for Modern Sciences and Arts

characteristic: collection date

text: Clinical isolate

characteristic: description

text: Egypt

text: diseased

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: Acinetobacter baumannii 20Y0075

characteristic: isolate

text: Endotracheal tube

text: Acinetobacter baumannii

characteristic: organism

text: Acinetobacter baumannii

characteristic: strain

characteristic: title


text: October University for Modern Sciences and Arts

characteristic: INSDC last update

text: public

text: ena-SAMPLE-TAB-02-12-2022-09:25:35:784-28384

characteristic: Submitter Id

text: Clinical isolate

text: Egypt

characteristic: geographic location (country and/or sea)

text: diseased

text: Homo sapiens

text: Acinetobacter baumannii 20Y0076

text: Sputum

text: Acinetobacter baumannii

text: Acinetobacter baumannii


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: ena-SAMPLE-TAB-02-12-2022-09:25:35:784-28385

text: Clinical isolate

text: Egypt

text: diseased

text: Homo sapiens

text: Acinetobacter baumannii 20Y0077

text: Wound swab

text: Acinetobacter baumannii

text: Acinetobacter baumannii


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ena-SAMPLE-TAB-02-12-2022-09:25:35:784-28386

text: Clinical isolate

text: Egypt

text: diseased

text: Homo sapiens

text: Acinetobacter baumannii 20Y0078

text: Blood

text: Acinetobacter baumannii

text: Acinetobacter baumannii


text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-02-12-2022-09:25:35:784-28387

characteristic: collection date

text: Clinical isolate

text: Egypt

text: diseased

text: Homo sapiens

text: Acinetobacter baumannii 20Y0079

text: Blood

text: Acinetobacter baumannii

text: Acinetobacter baumannii


text: public

text: ena-SAMPLE-TAB-02-12-2022-09:25:35:784-28388

text: Clinical isolate

characteristic: description

text: Egypt

text: diseased

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: Acinetobacter baumannii 20Y0080

text: Blood

text: Acinetobacter baumannii

text: Acinetobacter baumannii


text: public

text: ena-SAMPLE-TAB-02-12-2022-09:25:35:784-28389

text: Clinical isolate

text: Egypt

text: diseased

text: Homo sapiens

text: Acinetobacter baumannii 20Y0081

characteristic: isolate

text: Blood

text: Acinetobacter baumannii

characteristic: organism

text: Acinetobacter baumannii

characteristic: strain


characteristic: INSDC last update

text: public

text: ena-SAMPLE-TAB-02-12-2022-09:25:35:784-28390

characteristic: Submitter Id

text: Clinical isolate

text: Egypt

text: diseased

text: Homo sapiens

text: Acinetobacter baumannii 20Y0082

text: Blood

text: Acinetobacter baumannii

text: Acinetobacter baumannii

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: ena-SAMPLE-TAB-02-12-2022-09:25:35:784-28391

text: Clinical isolate

text: Egypt

characteristic: geographic location (country and/or sea)

text: diseased

text: Homo sapiens

text: Acinetobacter baumannii 20Y0083

text: Pus

text: Acinetobacter baumannii

text: Acinetobacter baumannii


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Shanghai Tenth People's Hosptial

characteristic: INSDC center name

text: public

tag: Namespace:Shanghai Tenth People's Hosptial

text: ArrayExpress

characteristic: broker name

text: Protocols: Strains were maintained at -80˚C. Strains were streaked on YPD plates. The plates were incubated at 37˚C overnight. Then several colonies were picked up and suspended in DDW. Cell density was adjusted to 10,000 cells/ml, then 100µl of cell suspension was plated on YPD plates. The plates were incubated at 37˚C overnight. Colonies were picked up and suspended in DDW, then cell pellets were obtained by centrifuging at highest speed for 1min. We used the phenol-chloroform method to extract Genomic DNA. Cell pellets were suspended in 200µl of lysis buffer, add 100µl of acid washed glass beads, and 0,2ml phenol:chloroform:isoamyl alcohol (25:24:1). Vortex for 30min at 4˚C. Add 200µl TE pH7.5. Centrifuge for 5min at maximum speed. Transfer aqueous layer to a new 1.5ml EP tube. Add 1ml 100% ethanol. Precipitate 1h at -20˚C. Pellet DNA by centrifuging for 10min at maximum speed. Discard the supernatant. Add 400µl TE pH 7.5. Add 3ul of RNase A (10mg/ml). Incubate at 37˚C for 30min. Add 10µl of 3M NaOAc and 1ml of 100% ethanol. Mix by inversion. Precipitate 30min at -20˚C. Pellet DNA by centrifuging for 10min at maximum speed. Discard supernatant. Air-dry the pellet overnight. Resuspend in 50µl water. 1µg Genomic DNA was randomly fragmented by Covaris, followed by fragments selection by Agencourt AMPure XP-Medium kit to an average size of 200~400 bp. Selected fragments were end repaired and 3\"adenylate, then the adaptors were ligated to the end of these 3\"adenylate fragments. The products were amplified by PCR and purified by the Agencourt AMPure XP-Medium kit. The purified double stranded PCR products were heat denatured to single strand, then circularised by the splint oligo sequence. The single strand circle DNA (ssCirDNA) were formatted as the final library and qualified by QC.

characteristic: genotype

text: YPD broth

characteristic: growth condition

text: not applicable

text: Cryptococcus neoformans var. grubii H99

text: Cryptococcus neoformans var. grubii H99

text: whole organism

characteristic: organism part

text: Cryptococcus neoformans var. grubii H99


text: Shanghai Tenth People's Hospital

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Shanghai Tenth People's Hospital

text: ArrayExpress

characteristic: description

text: wild type genotype

text: not applicable

text: Candida albicans

text: Candida albicans

text: Candida albicans


text: Shanghai Tenth People's Hospital

characteristic: INSDC last update

text: public

text: ArrayExpress

text: Aneuploid

text: not applicable

characteristic: isolate

text: Candida albicans

characteristic: organism

text: Candida albicans

text: Candida albicans


characteristic: ENA-CHECKLIST

text: STATENS SERUM INSTITUT

text: public

tag: Namespace:STATENS SERUM INSTITUT

characteristic: Submitter Id

text: uncultured bacterium

text: uncultured bacterium

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: DKFZ Heidelberg

text: public

tag: Namespace:DKFZ Heidelberg

text: human

characteristic: common name

text: untreated

text: Homo sapiens

text: Homo sapiens

text: untreated pull-down replicate 1


characteristic: External Id

text: DKFZ Heidelberg

characteristic: INSDC center name

text: public

text: human

text: untreated

text: Homo sapiens

text: Homo sapiens

text: untreated pull-down replicate 2


text: DKFZ Heidelberg

text: public

text: human

text: untreated

text: Homo sapiens

text: Homo sapiens

text: untreated XRNAX input replicate 1


text: DKFZ Heidelberg

text: public

text: human

text: untreated

text: Homo sapiens

text: Homo sapiens

text: untreated XRNAX input replicate 2


text: DKFZ Heidelberg

text: public

text: human

text: 400 uM arsenite 15 minutes

text: Homo sapiens

text: Homo sapiens

text: arsenite 15 min pull-down replicate 1


text: DKFZ Heidelberg

text: public

text: human

text: 400 uM arsenite 15 minutes

text: Homo sapiens

text: Homo sapiens

text: arsenite 15 min pull-down replicate 2


text: DKFZ Heidelberg

text: public

text: human

text: 400 uM arsenite 15 minutes

text: Homo sapiens

text: Homo sapiens

text: arsenite 15 min XRNAX input replicate 1


text: DKFZ Heidelberg

text: public

text: human

text: 400 uM arsenite 15 minutes

text: Homo sapiens

text: Homo sapiens

text: arsenite 15 min XRNAX input replicate 2


text: DKFZ Heidelberg

text: public

text: human

text: 400 uM arsenite 30 minutes

text: Homo sapiens

text: Homo sapiens

text: arsenite 30 min pull-down replicate 1


text: DKFZ Heidelberg

text: public

text: human

text: 400 uM arsenite 30 minutes

text: Homo sapiens

text: Homo sapiens

text: arsenite 30 min pull-down replicate 2


text: DKFZ Heidelberg

text: public

text: human

text: 400 uM arsenite 30 minutes

text: Homo sapiens

text: Homo sapiens

text: arsenite 30 min XRNAX input replicate 1


text: DKFZ Heidelberg

text: public

text: human

text: 400 uM arsenite 30 minutes

text: Homo sapiens

text: Homo sapiens

text: arsenite 30 min XRNAX input replicate 2


text: Shanghai Tenth People's Hospital

text: public

text: ArrayExpress

characteristic: broker name

text: wild type genotype

characteristic: genotype

text: YPD plate large colony

characteristic: growth condition

text: not applicable

text: wild type karyotype

characteristic: karyotype

text: Candida albicans

text: Candida albicans

text: whole organism

text: Candida albicans

characteristic: strain


text: Shanghai Tenth People's Hospital

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

characteristic: description

text: wild type genotype

text: YPD plate large colony

text: not applicable

text: euploid revertant

text: Candida albicans

text: Candida albicans

text: whole organism

characteristic: organism part

text: Candida albicans


characteristic: ENA-CHECKLIST

text: Shanghai Tenth People's Hospital

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: ArrayExpress

text: wild type genotype

text: YPD plate large colony

text: not applicable

characteristic: isolate

text: aneuploid

text: Candida albicans

characteristic: organism

text: Candida albicans

text: whole organism

text: Candida albicans

text: SegChr1x1 derived from F137 parent


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: ena-SAMPLE-TAB-15-12-2022-14:18:54:593-31388

tag: Namespace:Max Planck Institute for Marine Microbiology

text: Haloferax volcanii DS2

text: Haloferax volcanii DS2

characteristic: title


text: Max Planck Institute for Marine Microbiology

characteristic: INSDC center name

text: public

text: ena-SAMPLE-TAB-15-12-2022-14:18:54:593-31389

text: Haloferax volcanii DS2

text: Haloferax volcanii DS2


text: public

text: ena-SAMPLE-TAB-15-12-2022-14:18:54:593-31390

text: Haloferax volcanii DS2

text: Haloferax volcanii DS2


text: public

text: ena-SAMPLE-TAB-15-12-2022-14:18:54:593-31391

text: Haloferax volcanii DS2

text: Haloferax volcanii DS2


text: public

text: ena-SAMPLE-TAB-15-12-2022-14:18:54:593-31392

text: Haloferax volcanii DS2

text: Haloferax volcanii DS2


text: public

text: ena-SAMPLE-TAB-15-12-2022-14:18:54:593-31393

text: Haloferax volcanii DS2

text: Haloferax volcanii DS2


text: public

text: ena-SAMPLE-TAB-15-12-2022-14:18:54:593-31394

text: Haloferax volcanii DS2

text: Haloferax volcanii DS2


text: public

text: ena-SAMPLE-TAB-15-12-2022-14:18:54:593-31395

text: Haloferax volcanii DS2

text: Haloferax volcanii DS2


text: public

text: ena-SAMPLE-TAB-15-12-2022-14:18:54:593-31396

text: Haloferax volcanii DS2

text: Haloferax volcanii DS2


text: public

text: ena-SAMPLE-TAB-15-12-2022-14:18:54:593-31397

text: Haloferax volcanii DS2

text: Haloferax volcanii DS2


text: public

text: ena-SAMPLE-TAB-15-12-2022-14:18:54:593-31398

text: Haloferax volcanii DS2

text: Haloferax volcanii DS2


text: public

text: ena-SAMPLE-TAB-15-12-2022-14:18:54:593-31399

text: Haloferax volcanii DS2

text: Haloferax volcanii DS2


text: public

text: ena-SAMPLE-TAB-15-12-2022-14:18:54:593-31400

text: Haloferax volcanii DS2

text: Haloferax volcanii DS2


text: public

text: ena-SAMPLE-TAB-15-12-2022-14:18:54:593-31401

text: Haloferax volcanii DS2

text: Haloferax volcanii DS2


text: public

text: ena-SAMPLE-TAB-15-12-2022-14:18:54:593-31402

text: Haloferax volcanii DS2

text: Haloferax volcanii DS2


text: public

text: ena-SAMPLE-TAB-15-12-2022-14:18:54:593-31403

text: Haloferax volcanii DS2

text: Haloferax volcanii DS2


text: public

text: ena-SAMPLE-TAB-15-12-2022-14:18:54:593-31404

text: Haloferax volcanii DS2

text: Haloferax volcanii DS2


text: Max Planck Institute for Marine Microbiology

text: public

text: ena-SAMPLE-TAB-15-12-2022-14:18:54:594-31405

text: Haloferax volcanii DS2

text: Haloferax volcanii DS2


text: public

text: ena-SAMPLE-TAB-15-12-2022-14:18:54:594-31406

text: Haloferax volcanii DS2

text: Haloferax volcanii DS2


text: public

text: ena-SAMPLE-TAB-15-12-2022-14:18:54:594-31407

text: Haloferax volcanii DS2

text: Haloferax volcanii DS2


text: public

text: ena-SAMPLE-TAB-15-12-2022-14:18:54:594-31408

text: Haloferax volcanii DS2

text: Haloferax volcanii DS2


text: public

text: ena-SAMPLE-TAB-15-12-2022-14:18:54:594-31409

text: Haloferax volcanii DS2

text: Haloferax volcanii DS2


text: public

text: ena-SAMPLE-TAB-15-12-2022-14:18:54:594-31410

text: Haloferax volcanii DS2

text: Haloferax volcanii DS2


text: public

text: ena-SAMPLE-TAB-15-12-2022-14:18:54:594-31411

text: Haloferax volcanii DS2

text: Haloferax volcanii DS2


text: public

text: ena-SAMPLE-TAB-15-12-2022-14:18:54:594-31412

text: Haloferax volcanii DS2

text: Haloferax volcanii DS2


text: public

text: ena-SAMPLE-TAB-15-12-2022-14:18:54:594-31413

text: Haloferax volcanii DS2

text: Haloferax volcanii DS2


text: public

text: ena-SAMPLE-TAB-15-12-2022-14:18:54:594-31414

text: Haloferax volcanii DS2

text: Haloferax volcanii DS2


text: public

text: ena-SAMPLE-TAB-15-12-2022-14:18:54:594-31415

text: Haloferax volcanii DS2

text: Haloferax volcanii DS2


text: public

text: ena-SAMPLE-TAB-15-12-2022-14:18:54:594-31416

text: Haloferax volcanii DS2

text: Haloferax volcanii DS2


text: public

tag: Namespace:San Raffaele Telethon Institute for Gene Therapy

text: human

characteristic: common name

text: BE4max positive - Rep0

text: Homo sapiens

text: Homo sapiens


text: San Raffaele Telethon Institute for Gene Therapy

text: public

text: human

text: BE4max negative - Rep0

text: Homo sapiens

text: Homo sapiens


text: public

text: human

text: BE4max positive - Rep2

text: Homo sapiens

text: Homo sapiens


text: public

text: human

text: BE4max negative - Rep2

text: Homo sapiens

text: Homo sapiens


text: public

text: human

text: BE4max positive - Rep3

text: Homo sapiens

text: Homo sapiens


text: public

text: human

text: BE4max negative - Rep3

text: Homo sapiens

text: Homo sapiens


text: public

text: human

text: ABE8.20-m positive - Rep0

text: Homo sapiens

text: Homo sapiens


text: public

text: human

text: ABE8.20-m positive - Rep1

text: Homo sapiens

text: Homo sapiens


text: public

text: human

text: ABE8.20-m positive - Rep3

text: Homo sapiens

text: Homo sapiens


text: public

text: human

text: ABE8.20-m positive - Rep4

text: Homo sapiens

text: Homo sapiens


text: public

text: human

text: Cas9 - Rep0

text: Homo sapiens

text: Homo sapiens


text: public

text: human

text: Cas9 - Rep1

text: Homo sapiens

text: Homo sapiens


text: public

text: human

text: Mock electro - Rep0

text: Homo sapiens

text: Homo sapiens


text: public

text: human

text: Mock electro - Rep1

text: Homo sapiens

text: Homo sapiens


text: public

text: human

text: Mock electro - Rep3

text: Homo sapiens

text: Homo sapiens


text: public

tag: Namespace:Centre for Microbiology and Environmental Systems Science, University of Vienna

text: HDMEC

text: human

text: Uninfected HDMEC

text: Homo sapiens

text: Homo sapiens


text: Centre for Microbiology and Environmental Systems Science, University of Vienna

text: public

text: HDMEC

text: human

text: Uninfected HDMEC

text: Homo sapiens

text: Homo sapiens


text: public

text: HDMEC

text: human

text: Infected HDMEC with Neisseria meningitidis 2C4.3 for 3h

text: Homo sapiens

text: Homo sapiens


text: public

text: HBMEC

text: human

text: Uninfected HBMEC

text: Homo sapiens

text: Homo sapiens


text: public

text: HBMEC

text: human

text: Infected HBMEC with Neisseria meningitidis 2C4.3 for 3h

text: Homo sapiens

text: Homo sapiens


E-MTAB-12384:Sample 1

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12384:Sample 1

tag: Namespace:UNIVERSITY OF MANCHESTER

unit: month

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

text: Protocols: Male eight-week-old C57Bl/6N mice underwent transverse aortic constriction (TAC) or sham operations. Mice were terminated eight weeks post-surgery or when HF symptoms developed, and atrioventricular node (AV node) biopsies were collected. AV node biopsies taken from three mice were pooled and three such replicates were obtained from HF (TAC) and control (sham-operated) mice. Total RNA was isolated from the AV node biopsies using the RNeasy Micro kit (Qiagen) according to the manufacturer’s instructions. Total RNA was submitted to the Genomic Technologies Core Facility (University of Manchester). RNA quantity and integrity were measured using a 2200 TapeStation (Agilent Technologies). TrueSeq Stranded mRNA assay (Illumina) was used to generate libraries.

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

characteristic: individual

text: not applicable

text: Mus musculus

text: Mus musculus

text: atrioventricular node

text: Mus musculus

text: male

characteristic: sex

characteristic: strain

text: Sample 1


E-MTAB-12384:Sample 2

characteristic: ENA-CHECKLIST

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12384:Sample 2

text: ArrayExpress

text: house mouse

characteristic: description

text: adult

text: wild type genotype

text: not applicable

text: Mus musculus

text: Mus musculus

text: atrioventricular node

characteristic: organism part

text: Mus musculus

text: male

text: Sample 2


E-MTAB-12384:Sample 3

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12384:Sample 3

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: not applicable

characteristic: isolate

text: Mus musculus

characteristic: organism

text: Mus musculus

text: atrioventricular node

text: Mus musculus

text: male

text: Sample 3

characteristic: title


E-MTAB-12384:Sample 4

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

text: public

text: E-MTAB-12384:Sample 4

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

characteristic: individual

text: not applicable

text: Mus musculus

text: Mus musculus

text: atrioventricular node

text: Mus musculus

text: male

text: Sample 4


E-MTAB-12384:Sample 5

characteristic: ENA-CHECKLIST

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12384:Sample 5

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: not applicable

text: Mus musculus

text: Mus musculus

text: atrioventricular node

text: Mus musculus

text: male

characteristic: sex

characteristic: strain

text: Sample 5


E-MTAB-12384:Sample 6

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12384:Sample 6

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

characteristic: description

text: adult

text: wild type genotype

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: atrioventricular node

characteristic: organism part

text: Mus musculus

text: male

text: Sample 6


text: Max Planck Institute for Marine Microbiology

characteristic: INSDC center name

text: public

text: ena-SAMPLE-TAB-16-12-2022-14:45:40:597-51618

text: Halobacterium salinarum NRC-1

characteristic: organism

text: Halobacterium salinarum NRC-1

characteristic: title


characteristic: ENA-CHECKLIST

text: public

text: ena-SAMPLE-TAB-16-12-2022-14:45:40:597-51619

text: Halobacterium salinarum NRC-1

text: Halobacterium salinarum NRC-1


characteristic: INSDC first public

text: public

text: ena-SAMPLE-TAB-16-12-2022-14:45:40:597-51620

text: Halobacterium salinarum NRC-1

text: Halobacterium salinarum NRC-1


characteristic: INSDC last update

text: public

text: ena-SAMPLE-TAB-16-12-2022-14:45:40:597-51621

text: Halobacterium salinarum NRC-1

text: Halobacterium salinarum NRC-1


text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-16-12-2022-14:56:02:273-51623

text: Haloferax volcanii DS2

text: Haloferax volcanii DS2


text: public

characteristic: SRA accession

text: ena-SAMPLE-TAB-16-12-2022-14:56:02:273-51624

text: Haloferax volcanii DS2

text: Haloferax volcanii DS2


text: public

text: ena-SAMPLE-TAB-16-12-2022-14:56:02:273-51625

text: Haloferax volcanii DS2

text: Haloferax volcanii DS2


text: public

text: ena-SAMPLE-TAB-16-12-2022-14:56:02:273-51626

text: Haloferax volcanii DS2

text: Haloferax volcanii DS2


text: public

text: ena-SAMPLE-TAB-16-12-2022-14:56:02:273-51627

text: Haloferax volcanii DS2

text: Haloferax volcanii DS2


text: public

text: ena-SAMPLE-TAB-16-12-2022-14:56:02:273-51628

text: Haloferax volcanii DS2

text: Haloferax volcanii DS2


text: public

text: ena-SAMPLE-TAB-16-12-2022-14:56:02:273-51629

text: Haloferax volcanii DS2

text: Haloferax volcanii DS2


text: public

text: ena-SAMPLE-TAB-16-12-2022-14:56:02:273-51630

text: Haloferax volcanii DS2

text: Haloferax volcanii DS2


text: public

tag: Namespace:The Westmead Institute for Medical Research and Marie Bashir Institute for Infectious Diseases and Biosecurity

text: Isolate

text: Human respiratory syncytial virus A

text: Human respiratory syncytial virus A

text: HRSV-A


text: The Westmead Institute for Medical Research and Marie Bashir Institute for Infectious Diseases and Biosecurity

text: public

text: Isolate

text: Human respiratory syncytial virus A

text: Human respiratory syncytial virus A

text: HRSV-A


text: public

text: Isolate�

text: Human respiratory syncytial virus B

text: Human respiratory syncytial virus B

text: HRSV-B


text: Sciensano

text: public

text: ena-SAMPLE-TAB-19-12-2022-10:15:37:601-60922

text: Asian cultivated rice

characteristic: common name

text: Nipponbare

characteristic: cultivar

text: Gene-edited rice line 100% - Parental rice line 0%

text: Crispr/Cas-induced adenosine insertion in OsMADS26

characteristic: genetic modification

text: Oryza sativa

text: grain

characteristic: ploidy

text: Oryza sativa

text: 100% GMO rice


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Sciensano

text: public

text: ena-SAMPLE-TAB-19-12-2022-10:15:37:601-60923

text: Asian cultivated rice

text: Nipponbare

text: Gene-edited rice line 99.9% - Parental rice line 0.1%

text: Oryza sativa

text: grain

text: Oryza sativa

text: 99.9% GMO rice


text: Sciensano

text: public

text: ena-SAMPLE-TAB-19-12-2022-10:15:37:601-60924

characteristic: Submitter Id

text: Asian cultivated rice

text: Nipponbare

text: Gene-edited rice line 99.1% - Parental rice line 0.9%

characteristic: description

text: Oryza sativa

text: grain

text: Oryza sativa

text: 99.1% GMO rice


text: Sciensano

characteristic: INSDC center name

text: public

text: ena-SAMPLE-TAB-19-12-2022-10:15:37:601-60925

text: Asian cultivated rice

text: Nipponbare

text: Gene-edited rice line 95% - Parental rice line 5%

text: Oryza sativa

text: grain

characteristic: organism part

text: Oryza sativa

text: 95% GMO rice


text: Sciensano

text: public

text: ena-SAMPLE-TAB-19-12-2022-10:15:37:601-60926

text: Asian cultivated rice

text: Nipponbare

text: Gene-edited rice line 90% - Parental rice line 10%

text: Oryza sativa

characteristic: organism

text: grain

text: Oryza sativa

text: 90% GMO rice

characteristic: title


characteristic: ENA-CHECKLIST

text: Sciensano

characteristic: INSDC first public

text: public

text: ena-SAMPLE-TAB-19-12-2022-10:15:37:601-60927

text: Asian cultivated rice

text: Nipponbare

text: Gene-edited rice line 50% - Parental rice line 50%

text: Oryza sativa

text: grain

text: Oryza sativa

text: 50% GMO rice


text: Sciensano

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-19-12-2022-10:15:37:601-60928

text: Asian cultivated rice

text: Nipponbare

text: Gene-edited rice line 10% - Parental rice line 90%

text: Oryza sativa

text: grain

text: Oryza sativa

text: 10% GMO rice


text: Sciensano

text: public

characteristic: SRA accession

text: ena-SAMPLE-TAB-19-12-2022-10:15:37:601-60929

text: Asian cultivated rice

characteristic: common name

text: Nipponbare

text: Gene-edited rice line 5% - Parental rice line 95%

text: Oryza sativa

text: grain

text: Oryza sativa

text: 5% GMO rice


text: Sciensano

text: public

text: ena-SAMPLE-TAB-19-12-2022-10:15:37:601-60930

text: Asian cultivated rice

text: Nipponbare

characteristic: cultivar

text: Gene-edited rice line 0.9% - Parental rice line 99.1%

characteristic: genetic modification

text: Oryza sativa

text: grain

text: Oryza sativa

text: 0.9% GMO rice


characteristic: ENA-FIRST-PUBLIC

text: Sciensano

text: public

text: ena-SAMPLE-TAB-19-12-2022-10:15:37:601-60931

text: Asian cultivated rice

text: Nipponbare

text: Gene-edited rice line 0.1% - Parental rice line 99.9%

text: Crispr/Cas-induced adenosine insertion in OsMADS26

text: Oryza sativa

text: grain

characteristic: ploidy

text: Oryza sativa

text: 0.1% GMO rice


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Sciensano

text: public

text: ena-SAMPLE-TAB-19-12-2022-10:15:37:601-60932

text: Asian cultivated rice

text: Nipponbare

text: Gene-edited rice line 0% - Parental rice line 100%

text: Oryza sativa

text: grain

text: Oryza sativa

text: 100% WT rice


text: Sciensano

text: public

text: ena-SAMPLE-TAB-19-12-2022-10:15:37:601-60933

characteristic: Submitter Id

text: Asian cultivated rice

text: Nipponbare

text: Gene-edited rice noodles 100% - Parental rice noodles 0%

characteristic: description

text: Oryza sativa

text: grain

text: Oryza sativa

text: 100% GMO noodles


text: Sciensano

characteristic: INSDC center name

text: public

text: ena-SAMPLE-TAB-19-12-2022-10:15:37:601-60934

text: Asian cultivated rice

text: Nipponbare

text: Gene-edited rice noodles 99.9% - Parental rice noodles 0.1%

text: Oryza sativa

text: grain

characteristic: organism part

text: Oryza sativa

text: 99.9% GMO noodles


text: Sciensano

text: public

text: ena-SAMPLE-TAB-19-12-2022-10:15:37:601-60935

text: Asian cultivated rice

text: Nipponbare

text: Gene-edited rice noodles 99.1% - Parental rice noodles 0.9%

text: Oryza sativa

characteristic: organism

text: grain

text: Oryza sativa

text: 99.1% GMO noodles

characteristic: title


characteristic: ENA-CHECKLIST

text: Sciensano

characteristic: INSDC first public

text: public

text: ena-SAMPLE-TAB-19-12-2022-10:15:37:601-60936

text: Asian cultivated rice

text: Nipponbare

text: Gene-edited rice noodles 0.9% - Parental rice noodles line 99.1%

text: Oryza sativa

text: grain

text: Oryza sativa

text: 0.9% GMO noodles


text: Sciensano

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-19-12-2022-10:15:37:601-60937

text: Asian cultivated rice

text: Nipponbare

text: Gene-edited rice noodles 0.1% - Parental rice noodles 99.9%

text: Oryza sativa

text: grain

text: Oryza sativa

text: 0.1% GMO noodles


text: Sciensano

text: public

characteristic: SRA accession

text: ena-SAMPLE-TAB-19-12-2022-10:15:37:601-60938

text: Asian cultivated rice

characteristic: common name

text: Nipponbare

text: Gene-edited rice noodles 0% - Parental rice noodles 100%

text: Oryza sativa

text: grain

text: Oryza sativa

text: 100% WT noodles


text: Sciensano

text: public

text: ena-SAMPLE-TAB-19-12-2022-10:15:37:601-60939

text: Asian cultivated rice

text: Nipponbare

characteristic: cultivar

text: Gene-edited rice line 0.1% - Parental rice line 0.1% - Wild-type maize 99.8%

characteristic: genetic modification

text: Oryza sativa

text: grain

text: Oryza sativa

text: Mix 0.1% GMO- 0.1% WT rice and 99.8% maize


characteristic: ENA-FIRST-PUBLIC

text: Sciensano

text: public

text: ena-SAMPLE-TAB-19-12-2022-10:15:37:601-60940

text: Asian cultivated rice

text: Nipponbare

text: Gene-edited rice line 0.1% - Parental rice line 0.1% - Wild-type soybean 99.8%

text: Crispr/Cas-induced adenosine insertion in OsMADS26

text: Oryza sativa

text: grain

characteristic: ploidy

text: Oryza sativa

text: Mix 0.1% GMO- 0.1% WT rice and 99.8% soybean


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Complutense of Madrid

text: public

tag: Namespace:University Complutense of Madrid

text: cattle

text: Caruncle, uninfected sample, 10 dpi

text: PBS

characteristic: infection

text: Bos taurus

text: Live

text: Bos taurus

text: Female

characteristic: sex

text: Day 10

characteristic: timepoint

text: Caruncle


text: University Complutense of Madrid

characteristic: INSDC center name

text: public

characteristic: Submitter Id

text: cattle

text: Caruncle , Nc-Spain7 infected sample,10 dpi

characteristic: description

text: Bos taurus

characteristic: organism

text: Live

text: Bos taurus

text: Female

text: Day 10

text: Caruncle


characteristic: ENA-CHECKLIST

text: University Complutense of Madrid

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: cattle

text: Caruncle , Nc-Spain1H infected sample,10 dpi

text: Bos taurus

text: Live

text: Bos taurus

text: Female

text: Day 10

text: Caruncle

characteristic: title


text: University Complutense of Madrid

text: public

characteristic: INSDC status

characteristic: SRA accession

text: cattle

characteristic: common name

text: Cotyledon, uninfected sample, 10 dpi

text: PBS

text: Bos taurus

text: Live

text: Bos taurus

text: Female

text: Day 10

text: Cotyledon


characteristic: ENA-FIRST-PUBLIC

text: University Complutense of Madrid

text: public

text: cattle

text: Cotyledon , Nc-Spain7 infected sample,10 dpi

text: Bos taurus

text: Live

text: Bos taurus

text: Female

text: Day 10

text: Cotyledon


characteristic: ENA-LAST-UPDATE

text: University Complutense of Madrid

text: public

text: cattle

text: Cotyledon , Nc-Spain1H infected sample,10 dpi

text: Bos taurus

text: Live

text: Bos taurus

text: Female

text: Day 10

text: Cotyledon


characteristic: External Id

text: University Complutense of Madrid

text: public

text: cattle

text: Caruncle, uninfected sample, 20 dpi

text: PBS

text: Bos taurus

text: Live

text: Bos taurus

text: Female

text: Day 20

text: Caruncle


text: University Complutense of Madrid

text: public

text: cattle

text: Caruncle , Nc-Spain7 infected sample,20 dpi

characteristic: infection

text: Bos taurus

text: Death

text: Bos taurus

text: Female

text: Day 20

text: Caruncle


text: University Complutense of Madrid

text: public

text: cattle

text: Bos taurus

text: Live

text: Bos taurus

text: Female

characteristic: sex

text: Day 20

text: Caruncle


text: University Complutense of Madrid

text: public

text: cattle

text: Caruncle , Nc-Spain1H infected sample,20 dpi

text: Bos taurus

text: Live

text: Bos taurus

text: Female

text: Day 20

characteristic: timepoint

text: Caruncle


text: University Complutense of Madrid

characteristic: INSDC center name

text: public

text: cattle

text: Cotyledon, uninfected sample, 20 dpi

text: PBS

text: Bos taurus

text: Live

text: Bos taurus

text: Female

text: Day 20

text: Cotyledon


text: University Complutense of Madrid

text: public

characteristic: Submitter Id

text: cattle

text: Cotyledon , Nc-Spain7 infected sample,20 dpi

text: Bos taurus

text: Death

text: Bos taurus

text: Female

text: Day 20

text: Cotyledon


text: University Complutense of Madrid

text: public

text: cattle

characteristic: description

text: Bos taurus

text: Live

text: Bos taurus

text: Female

text: Day 20

text: Cotyledon


text: University Complutense of Madrid

text: public

text: cattle

text: Cotyledon , Nc-Spain1H infected sample,20 dpi

text: Bos taurus

characteristic: organism

text: Live

text: Bos taurus

text: Female

text: Day 20

text: Cotyledon


characteristic: ENA-CHECKLIST

text: IDIBELL

text: public

text: Bone Marrow

text: human

text: Bone marrow sample

text: Spain

characteristic: geographic location (country and/or sea)

text: Homo sapiens

text: Homo sapiens

text: Male


text: IDIBELL

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Saliva

text: human

text: Control sample

text: Spain

text: Homo sapiens

text: Homo sapiens

text: Male


text: IDIBELL

text: public

characteristic: INSDC status

text: Bone Marrow

text: human

text: Bone marrow sample

text: Spain

text: Homo sapiens

text: Homo sapiens

text: Female

characteristic: title


text: IDIBELL

text: public

characteristic: SRA accession

text: Fibroblasts

text: human

characteristic: common name

text: Control sample

text: Spain

text: Homo sapiens

text: Homo sapiens

text: Female


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: IDIBELL

text: public

text: Fibroblasts

text: human

text: Control sample

text: Spain

text: Homo sapiens

text: Homo sapiens

text: Male


characteristic: External Id

text: IDIBELL

text: public

text: Fibroblasts

text: human

text: Bone marrow sample

text: Spain

text: Homo sapiens

text: Homo sapiens

text: Male


text: IDIBELL

text: public

text: Peripheral Blood

text: human

text: Peripheral blood sample

text: Spain

text: Homo sapiens

text: Homo sapiens

text: Male

characteristic: sex


text: ISTITUTO ITALIANO DI TECNOLOGIA

text: public

tag: Namespace:ISTITUTO ITALIANO DI TECNOLOGIA

text: cultured cortical cells

text: house mouse

text: ChIP on mouse cortical cells infected with shCtrl with Ezh2 antibody replicate 1

text: Mus musculus

text: Mus musculus

characteristic: strain


text: ISTITUTO ITALIANO DI TECNOLOGIA

characteristic: INSDC center name

text: public

text: cultured cortical cells

text: house mouse

text: ChIP on mouse cortical cells infected with shCtrl with Ezh2 antibody replicate 2

text: Mus musculus

text: Mus musculus


text: ISTITUTO ITALIANO DI TECNOLOGIA

text: public

characteristic: Submitter Id

text: cultured cortical cells

text: house mouse

text: ChIP on mouse cortical cells infected with shL1-a with Ezh2 antibody replicate 1

text: Mus musculus

text: Mus musculus


text: ISTITUTO ITALIANO DI TECNOLOGIA

text: public

text: cultured cortical cells

text: house mouse

text: ChIP on mouse cortical cells infected with shL1-a with Ezh2 antibody replicate 2

characteristic: description

text: Mus musculus

text: Mus musculus


text: ISTITUTO ITALIANO DI TECNOLOGIA

text: public

text: cultured cortical cells

text: house mouse

text: ChIP on mouse cortical cells infected with shCtrl with H3K27me3 antibody replicate 1

text: Mus musculus

characteristic: organism

text: Mus musculus


characteristic: ENA-CHECKLIST

text: ISTITUTO ITALIANO DI TECNOLOGIA

text: public

text: cultured cortical cells

text: house mouse

text: ChIP on mouse cortical cells infected with shCtrl with H3K27me3 antibody replicate 2

text: Mus musculus

text: Mus musculus


text: ISTITUTO ITALIANO DI TECNOLOGIA

text: public

text: cultured cortical cells

text: house mouse

text: ChIP on mouse cortical cells infected with shL1-a with H3K27me3 antibody replicate 1

text: Mus musculus

text: Mus musculus


text: ISTITUTO ITALIANO DI TECNOLOGIA

text: public

text: cultured cortical cells

text: house mouse

text: ChIP on mouse cortical cells infected with shL1-a with H3K27me3 antibody replicate 2

text: Mus musculus

text: Mus musculus


text: ISTITUTO ITALIANO DI TECNOLOGIA

text: public

text: cultured cortical cells

text: house mouse

text: DNA purified from mouse cortical cells infected with shCtrl before immunoprecipitation replicate 1

text: Mus musculus

text: Mus musculus


text: ISTITUTO ITALIANO DI TECNOLOGIA

text: public

text: cultured cortical cells

text: house mouse

text: DNA purified from mouse cortical cells infected with shCtrl before immunoprecipitation replicate 2

text: Mus musculus

text: Mus musculus


text: ISTITUTO ITALIANO DI TECNOLOGIA

text: public

text: cultured cortical cells

text: house mouse

text: DNA purified from mouse cortical cells infected with shL1-a before immunoprecipitation replicate 1

text: Mus musculus

text: Mus musculus


text: ISTITUTO ITALIANO DI TECNOLOGIA

text: public

text: cultured cortical cells

text: house mouse

text: DNA purified from mouse cortical cells infected with shL1-a before immunoprecipitation replicate 2

text: Mus musculus

text: Mus musculus


text: ISTITUTO ITALIANO DI TECNOLOGIA

text: public

text: cultured cortical cells

text: house mouse

text: ChIP on mouse cortical cells infected with shCtrl with GFP antibody replicate 1

text: Mus musculus

text: Mus musculus


text: ISTITUTO ITALIANO DI TECNOLOGIA

text: public

text: cultured cortical cells

text: house mouse

text: ChIP on mouse cortical cells infected with shCtrl with GFP antibody replicate 2

text: Mus musculus

text: Mus musculus


text: ISTITUTO ITALIANO DI TECNOLOGIA

text: public

text: cultured cortical cells

text: house mouse

text: ChIP on mouse cortical cells infected with shL1-a with GFP antibody replicate 1

text: Mus musculus

text: Mus musculus


text: ISTITUTO ITALIANO DI TECNOLOGIA

text: public

text: cultured cortical cells

text: house mouse

text: ChIP on mouse cortical cells infected with shL1-a with GFP antibody replicate 2

text: Mus musculus

text: Mus musculus


SAC 1-1-3

text: valearen

text: public

text: SAC 1-1-3

text: human

text: Lower right molar from human remain

text: Argentina

characteristic: geographic location (country and/or sea)

text: Salitroso Lake, Santa Cruz

characteristic: geographic location (region and locality)

text: Homo sapiens

text: Homo sapiens

text: Tooth

text: SAC 1-1-3 (LS20)


SAC 1-1-4

text: valearen

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: SAC 1-1-4

text: human

text: Lower right molar from human remain

text: Argentina

text: Salitroso Lake, Santa Cruz

text: Homo sapiens

text: Homo sapiens

text: Tooth

text: SAC 1-1-4 (LS22)


text: Shanghai Tenth People's Hospital

text: public

characteristic: INSDC status

text: ArrayExpress

characteristic: broker name

text: Protocols: Strains were maintained at -80˚C. Strains were streaked on YPD plates. The plates were incubated at 37˚C overnight. Then several colonies were picked up and suspended in DDW. Cell density was adjusted to 10,000 cells/ml, then 100µl of cell suspension was plated on YPD plates. The plates were incubated at 37˚C overnight. Colonies were picked up and suspended in DDW, then cell pellets were obtained by centrifuging at highest speed for 1min. We used the phenol-chloroform method to extract Genomic DNA. Cell pellets were suspended in 200µl of lysis buffer, add 100µl of acid washed glass beads, and 0,2ml phenol:chloroform:isoamyl alcohol (25:24:1). Vortex for 30min at 4˚C. Add 200µl TE pH7.5. Centrifuge for 5min at maximum speed. Transfer aqueous layer to a new 1.5ml EP tube. Add 1ml 100% ethanol. Precipitate 1h at -20˚C. Pellet DNA by centrifuging for 10min at maximum speed. Discard the supernatant. Add 400µl TE pH 7.5. Add 3ul of RNase A (10mg/ml). Incubate at 37˚C for 30min. Add 10µl of 3M NaOAc and 1ml of 100% ethanol. Mix by inversion. Precipitate 30min at -20˚C. Pellet DNA by centrifuging for 10min at maximum speed. Discard supernatant. Air-dry the pellet overnight. Resuspend in 50µl water. 1µg Genomic DNA was randomly fragmented by Covaris, followed by fragments selection by Agencourt AMPure XP-Medium kit to an average size of 200~400 bp. Selected fragments were end repaired and 3\"adenylate, then the adaptors were ligated to the end of these 3\"adenylate fragments. The products were amplified by PCR and purified by the Agencourt AMPure XP-Medium kit. The purified double stranded PCR products were heat denatured to single strand, then circularised by the splint oligo sequence. The single strand circle DNA (ssCirDNA) were formatted as the final library and qualified by QC.

text: wild type genotype

characteristic: genotype

text: YPD broth

characteristic: growth condition

text: not applicable

characteristic: isolate

text: wild type karyotype

characteristic: karyotype

text: Cryptococcus neoformans var. grubii H99

text: Cryptococcus neoformans var. grubii H99

text: whole organism

characteristic: organism part

text: Cryptococcus neoformans var. grubii H99

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Shanghai Tenth People's Hospital

characteristic: INSDC center name

text: public

characteristic: SRA accession

text: ArrayExpress

text: YPD broth

text: not applicable

text: chromosome 4 disomy

text: Cryptococcus neoformans var. grubii H99

text: Cryptococcus neoformans var. grubii H99

text: whole organism

text: Cryptococcus neoformans var. grubii H99

characteristic: strain


characteristic: ENA-CHECKLIST

text: THE JACKSON LABORATORY

text: public

text: Instrument

characteristic: Instrument

tag: Namespace:THE JACKSON LABORATORY

characteristic: Submitter Id

characteristic: collection date

text: human

characteristic: common name

text: USA

characteristic: geographic location (country and/or sea)

text: size fractionation

text: GENOMIC

text: WGS

text: Homo sapiens

characteristic: organism

text: Homo sapiens


text: THE JACKSON LABORATORY

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Instrument

text: human

text: JAX_HiFi_HG02011_m64119_220104_202343.bam

text: USA

text: size fractionation

text: GENOMIC

text: WGS

text: Homo sapiens

text: Homo sapiens


text: THE JACKSON LABORATORY

text: public

text: Instrument

text: human

text: JAX_HiFi_HG02011_m64039_220121_204950.bam

text: USA

text: size fractionation

text: GENOMIC

text: WGS

text: Homo sapiens

text: Homo sapiens

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: THE JACKSON LABORATORY

text: public

text: Instrument

text: human

text: JAX_HiFi_HG02011_m64119_220114_220850.bam

text: USA

text: size fractionation

text: GENOMIC

text: WGS

text: Homo sapiens

text: Homo sapiens


characteristic: ENA-LAST-UPDATE

text: THE JACKSON LABORATORY

text: public

text: Instrument

text: human

text: JAX_HiFi_HG02492_m64119_220206_072658.bam

text: USA

text: size fractionation

text: GENOMIC

text: WGS

text: Homo sapiens

text: Homo sapiens


characteristic: External Id

text: THE JACKSON LABORATORY

text: public

text: Instrument

text: human

text: JAX_HiFi_HG02492_m64039_220221_051108.bam

text: USA

text: size fractionation

text: GENOMIC

text: WGS

text: Homo sapiens

text: Homo sapiens


text: THE JACKSON LABORATORY

characteristic: INSDC center name

text: public

text: Instrument

text: human

text: JAX_HiFi_HG02492_m64119_220226_041620.bam

text: USA

text: size fractionation

text: GENOMIC

text: WGS

text: Homo sapiens

text: Homo sapiens


text: Shanghai Tenth People's Hospital

text: public

characteristic: SRA accession

characteristic: adapter library

text: ArrayExpress

characteristic: broker name

text: Protocols: Strains were maintained at -80˚C. Strains were streaked on YPD plates. The plates were incubated at 37˚C overnight. Then several colonies were picked up and suspended in DDW. Cell density was adjusted to 10,000 cells/ml, then 100µl of cell suspension was plated on YPD plates. The plates were incubated at 37˚C overnight. Colonies were picked up and suspended in DDW, then cell pellets were obtained by centrifuging at highest speed for 1min. We used the phenol-chloroform method to extract Genomic DNA. Cell pellets were suspended in 200µl of lysis buffer, add 100µl of acid washed glass beads, and 0,2ml phenol:chloroform:isoamyl alcohol (25:24:1). Vortex for 30min at 4˚C. Add 200µl TE pH7.5. Centrifuge for 5min at maximum speed. Transfer aqueous layer to a new 1.5ml EP tube. Add 1ml 100% ethanol. Precipitate 1h at -20˚C. Pellet DNA by centrifuging for 10min at maximum speed. Discard the supernatant. Add 400µl TE pH 7.5. Add 3ul of RNase A (10mg/ml). Incubate at 37˚C for 30min. Add 10µl of 3M NaOAc and 1ml of 100% ethanol. Mix by inversion. Precipitate 30min at -20˚C. Pellet DNA by centrifuging for 10min at maximum speed. Discard supernatant. Air-dry the pellet overnight. Resuspend in 50µl water. "1µg Genomic DNA was randomly fragmented by Covaris, followed by fragments selection by Agencourt AMPure XP-Medium kit to an average size of 200~400 bp. Selected fragments were end repaired and 3\""adenylate, then the adaptors were ligated to the end of these 3\""adenylate fragments. The products were amplified by PCR and purified by the Agencourt AMPure XP-Medium kit. The purified double stranded PCR products were heat denatured to single strand, then circularised by the splint oligo sequence. The single strand circle DNA (ssCirDNA) were formatted as the final library and qualified by QC."

characteristic: description

text: wild type genotype

characteristic: genotype

text: not applicable

characteristic: isolate

text: Candida auris

text: [Candida] auris

text: [Candida] auris

unit: degree celsius

characteristic: temperature


characteristic: ENA-CHECKLIST

text: Shanghai Tenth People's Hospital

text: public

characteristic: Submitter Id

text: ArrayExpress

text: aneuploid type

text: not applicable

text: Candida auris

text: [Candida] auris

text: [Candida] auris

characteristic: strain


characteristic: INSDC first public

characteristic: INSDC last update

text: public

tag: Namespace:Kennedy Institute of Rheumatology, University of Oxford

text: 14 to 15

unit: week

characteristic: age

text: ArrayExpress

text: large adipocyte

text: house mouse

characteristic: common name

text: Protocols: Mice (3 male + 3 female per experimental group) were treated with water or 2% DSS for 5 days and then maintained at water for 2 more days until mice were sacrificed at day 7. Visceral adipose tissues (epididymal, retroperitoneal and omental) from each mouse were pooled. Adipose tissues were collected and digested in DMEM containing 1% fatty acid-free BSA (Sigma, 126609), 5% HEPES (Gibco, 15630-056), 0.2mg/mL Liberase TL (Roche, 5401020001) and 20µg/mL DNaseI (Roche, 11284932001). Tissues were minced in digestion medium and incubated for 25-30min at 37°C at 180rpm. Tissues were further broken down by pipetting using wide-bore tips and filtered through a 100µm mesh. Digestion was quenched by adding medium containing 2mM EDTA. Adipocyte and stromal vascular fraction were separated by centrifugation (700g, 10min). Floating adipocyte fraction was removed and collected in a fresh tube. After 5min rest, subnatant (under floating adipocyte layer) was aspired and remaining adipocytes taken up in 1mL TRI reagent (T9424, Sigma). Total RNA was extracted using TRI reagent (T9424, Sigma) and chloroform following RNeasy Mini Kit (74104, Qiagen) manufacturer instructions. RNA was eluded in RNase-free water and stored at -80°C until further processing. Material was quantified using RiboGreen (Invitrogen) on the FLUOstar OPTIMA plate reader (BMG Labtech) and the size profile and integrity analysed on the 2200 or 4200 TapeStation (Agilent, RNA ScreenTape). RIN estimates for all samples were between 7.3 and 9.5. Input material was normalised to 100 ng prior to library preparation. Polyadenylated transcript enrichment and strand specific library preparation was completed using NEBNext Ultra II mRNA kit (NEB) following manufacturer’s instructions. Libraries were amplified (14 cycles) on a Tetrad (Bio-Rad) using in-house unique dual indexing primers (based on DOI: 10.1186/1472-6750-13-104).

text: adult

characteristic: developmental stage

text: intestinal disease

characteristic: disease

text: Atg7 knockout

characteristic: individual

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: adipose tissue

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Kennedy Institute of Rheumatology, University of Oxford

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 14 to 15

text: ArrayExpress

characteristic: broker name

text: large adipocyte

text: house mouse

characteristic: description

text: adult

text: intestinal disease

text: Atg7 knockout

text: not applicable

text: Mus musculus

text: Mus musculus

text: adipose tissue

text: Mus musculus

text: female

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: 14 to 15

characteristic: age

text: ArrayExpress

text: large adipocyte

text: house mouse

text: adult

text: intestinal disease

text: wild type genotype

characteristic: genotype

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: adipose tissue

text: Mus musculus

text: male

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: public

text: 14 to 15

text: ArrayExpress

text: large adipocyte

text: house mouse

characteristic: common name

text: adult

characteristic: developmental stage

text: intestinal disease

characteristic: disease

text: Atg7 knockout

characteristic: individual

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: adipose tissue

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 14 to 15

text: ArrayExpress

characteristic: broker name

text: large adipocyte

text: house mouse

characteristic: description

text: adult

text: intestinal disease

text: wild type genotype

text: not applicable

text: Mus musculus

text: Mus musculus

text: adipose tissue

text: Mus musculus

text: female

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: 14 to 15

characteristic: age

text: ArrayExpress

text: large adipocyte

text: house mouse

text: adult

text: normal

text: wild type genotype

characteristic: genotype

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: adipose tissue

text: Mus musculus

text: male

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: public

text: 14 to 15

text: ArrayExpress

text: large adipocyte

text: house mouse

characteristic: common name

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Atg7 knockout

characteristic: individual

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: adipose tissue

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 14 to 15

text: ArrayExpress

characteristic: broker name

text: large adipocyte

text: house mouse

characteristic: description

text: adult

text: normal

text: Atg7 knockout

text: not applicable

text: Mus musculus

text: Mus musculus

text: adipose tissue

text: Mus musculus

text: female

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: 14 to 15

characteristic: age

text: ArrayExpress

text: large adipocyte

text: house mouse

text: adult

text: normal

text: wild type genotype

characteristic: genotype

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: adipose tissue

text: Mus musculus

text: female

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: OLivier

text: public

text: Ward, C.J., 9831, (K)

text: Combretum sylvicola

characteristic: organism

text: Combretum sylvicola

text: Combretum sylvicola


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: OLivier

text: public

text: Sasaki, D., 478, (K); K001108959

text: Combretum pyramidatum

text: Combretum pyramidatum

text: Combretum pyramidatum


text: OLivier

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: Lace, J.H., 4563, (K)

text: Combretum deciduum

text: Combretum deciduum

text: Combretum deciduum


text: OLivier

text: public

characteristic: SRA accession

text: Thompson, E.J., 269, (BRI); AQ0727309

text: Terminalia subacroptera

text: Terminalia subacroptera

text: Terminalia subacroptera


text: OLivier

text: public

text: Roberts, A.S., 108, (K)

characteristic: description

text: Combretum trifoliatum

text: Combretum trifoliatum

text: Combretum trifoliatum


text: OLivier

text: public

text: Westaway, J., 4433, (CANB); CANB817811

text: Terminalia erythrocarpa

text: Terminalia erythrocarpa

text: Terminalia erythrocarpa

characteristic: title


characteristic: ENA-CHECKLIST

text: OLivier

text: public

text: Chavarria, U., 1107, (K)

text: Combretum decandrum

text: Combretum decandrum

text: Combretum decandrum


text: OLivier

characteristic: INSDC first public

text: public

text: Smith, A.C, 6441, (K); K000786520

text: Terminalia simulans

text: Terminalia simulans

text: Terminalia simulans


text: OLivier

characteristic: INSDC last update

text: public

text: Tucker, J.M., 446, (K)

text: Combretum argenteum

text: Combretum argenteum

text: Combretum argenteum


text: OLivier

text: public

characteristic: Submitter Id

text: Berry, M.J., 17, (K)

text: Terminalia strigillosa

text: Terminalia strigillosa

text: Terminalia strigillosa


text: OLivier

text: public

text: Jongking, C., 4069, (N)

text: Combretum oyemense

text: Combretum oyemense

text: Combretum oyemense


text: OLivier

text: public

text: Loudon, B., BLAS 03, (PERTH); PERTH8470685

text: Terminalia supranitifolia

text: Terminalia supranitifolia

text: Terminalia supranitifolia


text: OLivier

text: public

text: Cowie, I., 4268, (CANB); CANB494390

text: Terminalia cunninghamii

text: Terminalia cunninghamii

text: Terminalia cunninghamii


text: OLivier

text: public

text: Jongking, C., 4389, (N)

text: Combretum demeusei

text: Combretum demeusei

text: Combretum demeusei


text: OLivier

text: public

text: Forrest, G., 9580, (K)

text: Combretum roxburghii

text: Combretum roxburghii

text: Combretum roxburghii


text: OLivier

text: public

text: Edinger, D.J., 746, (PERTH); PERTH1166271

text: Terminalia fitzgeraldii

text: Terminalia fitzgeraldii

text: Terminalia fitzgeraldii


text: OLivier

text: public

text: Ferreira, 205, (K); K001244337

text: Terminalia oxycarpa

text: Terminalia oxycarpa

text: Terminalia oxycarpa


text: OLivier

text: public

text: Latz, P.K, 23185, (K)

text: Terminalia hadleyana subsp. carpentariae


text: OLivier

text: public

text: Sweedman, L., LSWE8913

text: Terminalia grandiflora

text: Terminalia grandiflora

text: Terminalia grandiflora


text: OLivier

text: public

text: Teixeira, L.O.A, 819, (K)

text: Combretum gracile

text: Combretum gracile

text: Combretum gracile


text: OLivier

text: public

text: Pearson, S., 612, (BRI); BRI AQ0657986

text: Macropteranthes fitzalanii

text: Macropteranthes fitzalanii

text: Macropteranthes fitzalanii


text: OLivier

text: public

text: Tressens, S.G., 2801, (K)

text: Terminalia australis

text: Terminalia australis

text: Terminalia australis


text: OLivier

text: public

text: Chapama, H.T., 858, (K)

text: Terminalia kaiseriana

text: Terminalia kaiseriana

text: Terminalia kaiseriana


text: OLivier

text: public

text: Oldeman, R.A.A., 832, (K)

text: Combretum paradoxum

text: Combretum paradoxum

text: Combretum paradoxum


text: OLivier

text: public

text: Fanshawe, D.B., 5058, (K)

text: Combretum glabrum

text: Combretum glabrum

text: Combretum glabrum


text: OLivier

text: public

text: Gauthier, L., 4193, (K)

text: Terminalia urschii

text: Terminalia urschii

text: Terminalia urschii


text: OLivier

text: public

text: Andriamahay, M, 1503, (K)

text: Terminalia subserrata

text: Terminalia subserrata

text: Terminalia subserrata


text: OLivier

text: public

text: Johns, 10427

text: Terminalia capitulata

text: Terminalia capitulata

text: Terminalia capitulata


text: OLivier

text: public

text: Fay, M., 4300, (K)

text: Combretum sericeum

text: Combretum sericeum

text: Combretum sericeum


text: OLivier

text: public

text: Yuncker, T.G., 8426, (K)

text: Combretum rovirosae

text: Combretum rovirosae

text: Combretum rovirosae


text: OLivier

text: public

text: Maurin, O., 4298, (K)

text: Terminalia gracilipes

text: Terminalia gracilipes

text: Terminalia gracilipes


text: OLivier

text: public

text: Mooney, H.F., 3313, (K)

text: Combretum nanum

text: Combretum nanum

text: Combretum nanum


text: OLivier

text: public

text: Eddowes, P.J., 38101, (K)

text: Terminalia eddowesii

text: Terminalia eddowesii

text: Terminalia eddowesii


text: OLivier

text: public

text: Mori, S.A., 17631, (K); K001108785

text: Combretum rohrii

text: Combretum rohrii

text: Combretum rohrii


text: OLivier

text: public

text: McCormick, G.D., s.n., (K)

text: Terminalia glabrata

text: Terminalia glabrata

text: Terminalia glabrata


text: OLivier

text: public

text: Maurin, O., 1211, (BNRH)

text: Terminalia gazensis

text: Terminalia gazensis

text: Terminalia gazensis


text: OLivier

text: public

text: Fonseca, M.R., 1308, (K)

text: Combretum hilarianum

text: Combretum hilarianum

text: Combretum hilarianum


text: OLivier

text: public

text: Ikechukwu, A., 86, (UNIPORT)

text: Combretum racemosum

text: Combretum racemosum

text: Combretum racemosum


text: OLivier

text: public

text: Slee, A.V., 3368, (CANB); CANB744953

text: Terminalia oblongata

text: Terminalia oblongata

text: Terminalia oblongata


text: OLivier

text: public

text: Ikechukwu, A., 87, (UNIPORT)

text: Combretum platypterum

text: Combretum platypterum

text: Combretum platypterum


text: OLivier

text: public

text: Briggs, J.D., 799, (CBG); CBG8307508

text: Terminalia latipes

text: Terminalia latipes

text: Terminalia latipes


text: OLivier

text: public

text: Cheng, S.K., 1286, (K)

text: Combretum quadrangulare

text: Combretum quadrangulare

text: Combretum quadrangulare


text: OLivier

text: public

text: Ikechukwu, A., 85, (UNIPORT)

text: Combretum zenkeri

text: Combretum zenkeri

text: Combretum zenkeri


text: OLivier

text: public

text: de Wilde, J.J.F.E., 8161, (K)

text: Strephonema polybotryum

text: Strephonema polybotryum

text: Strephonema polybotryum


text: OLivier

text: public

text: Prance, G.T., 2970, (K); K001108810

text: Combretum llewelynii

text: Combretum llewelynii

text: Combretum llewelynii


text: OLivier

text: public

text: Takeuchi, W., 6724

text: Terminalia canaliculata

text: Terminalia canaliculata

text: Terminalia canaliculata


text: public

tag: Namespace:Department of Ecology and Genetics, Uppsala University

text: larval abdomen

text: Leptidea sinapis

text: Leptidea sinapis


text: Department of Ecology and Genetics, Uppsala University

text: public

text: larval abdomen

text: Leptidea sinapis

text: Leptidea sinapis


text: public

text: adult abdomen

text: Leptidea sinapis

text: Leptidea sinapis


text: Plasmodium vivax

characteristic: ArrayExpress-SPECIES

text: SC

text: public

text: malaria parasite P. vivax

characteristic: common name

text: Plasmodium vivax

text: Plasmodium vivax


text: Martin-Luther-University Halle-Wittenberg

text: public

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

text: human

text: Protocols: Peripheral blood of patients with germline TNFAIP3 mutations and healthy control individuals was collected. Peripheral blood mononuclear cells (PBMCs) were isolated from peripheral blood by density-gradient centrifugation using Biocoll separation solution. Genomic DNA of PBMCs and lymphoma tissue was isolated using the GenElute mammalian genomic DNA miniprep kit The rearranged V, D and J gene segments of the IGH and TRB loci were amplified in a multiplex PCR using BIOMED2-FR1 (IGH) or -TRB-B primer pools together and 250 ng of genomic DNA. The primers were purchased from Metabion International AG. As described in17, two consecutive PCR reactions were performed to generate IGH and TRB fragments tagged with Illumina-compatible adapters.

text: adult

characteristic: developmental stage

text: A20 haploinsufficiency

characteristic: disease

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

characteristic: organism part

text: Homo sapiens

characteristic: sex


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: ArrayExpress

text: mononuclear cell

text: human

text: A20 haploinsufficiency

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: peripheral blood

text: Homo sapiens


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: ArrayExpress

text: mononuclear cell

text: human

characteristic: description

text: juvenile stage

text: A20 haploinsufficiency

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: Homo sapiens

characteristic: title


text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: Homo sapiens

text: female


characteristic: ENA-FIRST-PUBLIC

text: public

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: normal

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: ArrayExpress

text: mononuclear cell

text: human

text: infant

text: normal

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: peripheral blood

text: Homo sapiens

text: male


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: ArrayExpress

text: mononuclear cell

text: human

characteristic: description

text: juvenile stage

text: normal

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: Homo sapiens

text: female


text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

text: human

characteristic: common name

text: juvenile stage

characteristic: developmental stage

text: normal

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: Homo sapiens

text: male

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: public

text: ArrayExpress

text: B cell

text: human

text: Protocols: Peripheral blood mononuclear cells (PBMCs) were isolated from peripheral blood by density-gradient centrifugation using Biocoll separation solution (Biochrom AG, Berlin, Germany). PBMCs were sorted for live cells or for IGHV4-34 and non-IGHV4-34 B cells using an anti-IGHV4-34 antibody (9g4, IgmBiosciences, Mountain View, California, USA). Genomic DNA of PBMCs was isolated using the GenElute mammalian genomic DNA miniprep kit (Sigma-Aldrich, Taufkirchen, Germany) according to the manufacturer’s instructions. Chromium Single Cell 5' Library Construction Kit

text: adult

text: normal

characteristic: disease

text: IGHV4-34 positive

characteristic: immunophenotype

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens


E-MTAB-12485:non VH4-34 Bcells

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: E-MTAB-12485:non VH4-34 Bcells

text: ArrayExpress

text: B cell

text: human

text: adult

text: normal

text: IGHV4-34 negative

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: non VH4-34 Bcells


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: normal

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: Homo sapiens

text: healthy


text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

text: human

characteristic: description

text: adult

text: A20 haploinsufficiency

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens


E-MTAB-12517:Sample 1

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-12517:Sample 1

tag: Namespace:Department of Biomedical Sciences, University of Copenhagen, Denmark

text: ArrayExpress

text: embryonic stem cell

text: human

characteristic: common name

text: Protocols: The mesoderm differentiation protocol used in this dataset is a combination of published protocols that were further modified. These will be detailed in the corresponding publication After medium removal, cells were washed with PBS and scraped off in RLT buffer (QIAGEN RNeasy kit). QIAGEN RNeasy kit. Library was constructed with NEBNext Ultra II RNA library Prep Kit (New England BioLabs) using 120 ng of RNA. 9 cycles of PCR amplification were performed, and AMPure XP (Beckman Coulter) was used for cleanup. The quality was assessed using Fragment Analyzer (Agilent) and the concentration of cDNAs was measured using Qubit Fluorometer (Invitrogen).

text: embryo

characteristic: developmental stage

text: normal

text: wild type genotype

characteristic: genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: Homo sapiens

text: Sample 1

characteristic: title


E-MTAB-12517:Sample 10

tag: attribute

characteristic: ENA-LAST-UPDATE

text: Department of Biomedical Sciences, University of Copenhagen, Denmark

text: public

text: E-MTAB-12517:Sample 10

text: ArrayExpress

text: embryonic stem cell

text: human

text: embryo

text: normal

characteristic: disease

text: wild type genotype

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: Homo sapiens

text: Sample 10


E-MTAB-12517:Sample 2

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: E-MTAB-12517:Sample 2

text: ArrayExpress

text: embryonic stem cell

text: human

text: embryo

text: normal

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: Homo sapiens

text: Sample 2


E-MTAB-12517:Sample 3

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-12517:Sample 3

text: ArrayExpress

text: embryonic stem cell

text: human

text: embryo

text: normal

text: wild type genotype

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

text: Homo sapiens

text: Sample 3


E-MTAB-12517:Sample 4

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-12517:Sample 4

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: embryonic stem cell

text: human

characteristic: description

text: embryo

text: normal

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: Homo sapiens

text: Sample 4


E-MTAB-12517:Sample 5

text: public

text: E-MTAB-12517:Sample 5

text: ArrayExpress

text: embryonic stem cell

text: human

characteristic: common name

text: embryo

characteristic: developmental stage

text: normal

text: wild type genotype

characteristic: genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: Homo sapiens

text: Sample 5

characteristic: title


E-MTAB-12517:Sample 6

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-12517:Sample 6

text: ArrayExpress

text: embryonic stem cell

text: human

text: embryo

text: normal

characteristic: disease

text: wild type genotype

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: Homo sapiens

text: Sample 6


E-MTAB-12517:Sample 7

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: E-MTAB-12517:Sample 7

text: ArrayExpress

text: embryonic stem cell

text: human

text: embryo

text: normal

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: Homo sapiens

text: Sample 7


E-MTAB-12517:Sample 8

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-12517:Sample 8

text: ArrayExpress

text: embryonic stem cell

text: human

text: embryo

text: normal

text: wild type genotype

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

text: Homo sapiens

text: Sample 8


E-MTAB-12517:Sample 9

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-12517:Sample 9

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: embryonic stem cell

text: human

characteristic: description

text: embryo

text: normal

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: Homo sapiens

text: Sample 9


text: Hebrew University, Genetics Department

text: public

tag: Namespace:Hebrew University, Genetics Department

text: ArrayExpress

text: endoderm cell

text: human

characteristic: common name

text: Protocols: Cell culture and maintainance: For the genetic screen the haploid hESC line, h-pES10, was used to generate a genome-wide loss-of-function library. The mutant haploid hESC library was cultured at 37 °C and 5% CO2 in feeder-free conditions on matrigel-coated plates (Corning) in mTeSR1 (STEMCELL Technologies) supplemented with 10 μM ROCK inhibitor Y-27632 (Stemgent) for 1 day after splitting. Before reaching confluency, cells were passaged by a quick trypsinization using TrypLE Select (Thermo Fisher Scientific). Vero E6 cells were maintained in Eagle's Minimum Essential Medium (EMEM; Biological Industries, Beit Haemek, Israel), supplemented with 10% fetal bovine serum, 2 mM L-Glutamine, 10 IU/ml Penicillin, and 10 μg/ml streptomycin (Biological Industries, Beit Haemek, Israel). Definitive endoderm differentiation assay: Mutant h-pES10 library cells were used for differentiation into definitive endoderm (DE). Cells were plated on Matrigel-coated 6-well plates at a density of 2 million cells per well. The next day, STEMdiff™ Definitive Endoderm Kit (STEMCELL Technologies, cat. no. 05110) has been used according to manufacturer’s instructions. Briefly, the differentiation medium (2 ml/well) with its appropriate supplements was replaced every 24 hours for 4 days. SARS-CoV-2 Screen: On the 5th day of differentiation, differentiated DE cells were infected with SARS-CoV-2 at various MOIs. Following infection, cells were continued to be cultured in the DE medium for additional 5 days. Cells were allowed to undergo viral cytotoxicity and harvested 5 days post-infection for further analysis. 2 screen samples were infected with SARS-COV-2, and one control screen sample was not infected. Samples were harvested using trypsin gSYNC DNA extraction kit (Geneaid) PCR with Nextera overhangs

text: embryo

characteristic: developmental stage

text: normal

text: CRISPR/Cas9 loss of function library

characteristic: genotype

characteristic: infect

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: ctrl

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Hebrew University, Genetics Department

text: public

text: ArrayExpress

text: endoderm cell

text: human

text: embryo

text: normal

characteristic: disease

text: CRISPR/Cas9 loss of function library

text: Severe acute respiratory syndrome coronavirus 2

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: embryonic stem cell

text: Homo sapiens


E-MTAB-12528:Patient-1 Blood

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12528:Patient-1 Blood

tag: Namespace:Bioinformatics Core Facility University of Manchester

unit: year

characteristic: age

text: ArrayExpress

text: blood cell

text: human

text: Protocols: Primary tumour samples dissociated into single cell suspension after debris removal step. PBMCs extracted with Ficoll gradient seperation Used 10X sample collection protocol, detailed in CG000053_CellPrepGuide_RevC.pdfc Used 10X sample collection protocol, detailed in CG000053_CellPrepGuide_RevC.pdf

text: adult

text: low grade astrocytic tumor

characteristic: individual

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

characteristic: sampling site

text: Homo sapiens

text: male

characteristic: sex

text: Patient-1 Blood


E-MTAB-12528:Patient-1 Core

characteristic: ENA-FIRST-PUBLIC

text: Bioinformatics Core Facility University of Manchester

text: public

text: E-MTAB-12528:Patient-1 Core

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: microglial cell

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: low grade astrocytic tumor

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: brain

text: normal tissue adjacent to neoplasm

text: Homo sapiens

text: male

text: Patient-1 Core

characteristic: title


E-MTAB-12528:Patient-1 Tumour

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-12528:Patient-1 Tumour

text: ArrayExpress

text: microglial cell

text: human

text: adult

text: low grade astrocytic tumor

characteristic: disease

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: brain

characteristic: organism part

text: neoplasm

text: Homo sapiens

text: male

text: Patient-1 Tumour


E-MTAB-12528:Patient-2 Blood

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12528:Patient-2 Blood

characteristic: age

text: ArrayExpress

text: blood cell

text: human

text: adult

text: high grade astrocytic tumor

characteristic: individual

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: Homo sapiens

text: male

text: Patient-2 Blood


E-MTAB-12528:Patient-2 Core

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-12528:Patient-2 Core

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: microglial cell

text: human

characteristic: common name

characteristic: description

text: adult

text: high grade astrocytic tumor

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: brain

text: normal tissue adjacent to neoplasm

characteristic: sampling site

text: Homo sapiens

text: male

characteristic: sex

text: Patient-2 Core


E-MTAB-12528:Patient-2 Tumour

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-12528:Patient-2 Tumour

text: ArrayExpress

text: microglial cell

text: human

text: adult

characteristic: developmental stage

text: high grade astrocytic tumor

characteristic: disease

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: brain

text: neoplasm

text: Homo sapiens

text: male

text: Patient-2 Tumour

characteristic: title


E-MTAB-12528:Patient-3 Blood

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12528:Patient-3 Blood

characteristic: age

text: ArrayExpress

text: blood cell

text: human

text: adult

text: high grade astrocytic tumor

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens

text: male

text: Patient-3 Blood


E-MTAB-12528:Patient-3 Core

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-12528:Patient-3 Core

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: microglial cell

text: human

characteristic: common name

characteristic: description

text: adult

text: high grade astrocytic tumor

characteristic: individual

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: brain

text: normal tissue adjacent to neoplasm

text: Homo sapiens

text: male

text: Patient-3 Core


E-MTAB-12528:Patient-3 Tumour

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-12528:Patient-3 Tumour

text: ArrayExpress

text: microglial cell

text: human

text: adult

characteristic: developmental stage

text: high grade astrocytic tumor

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: brain

text: neoplasm

characteristic: sampling site

text: Homo sapiens

text: male

characteristic: sex

text: Patient-3 Tumour


E-MTAB-12528:Patient-4 Blood

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12528:Patient-4 Blood

characteristic: age

text: ArrayExpress

text: blood cell

text: human

text: adult

text: anaplastic astrocytoma

characteristic: disease

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: male

text: Patient-4 Blood

characteristic: title


E-MTAB-12528:Patient-4 Core

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-12528:Patient-4 Core

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: microglial cell

text: human

characteristic: common name

characteristic: description

text: adult

text: anaplastic astrocytoma

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: brain

characteristic: organism part

text: normal tissue adjacent to neoplasm

text: Homo sapiens

text: male

text: Patient-4 Core


E-MTAB-12528:Patient-4 White    Matter

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-12528:Patient-4 White Matter

text: ArrayExpress

text: microglial cell

text: human

text: adult

characteristic: developmental stage

text: anaplastic astrocytoma

characteristic: individual

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: brain

text: neoplasm

text: Homo sapiens

text: male

text: Patient-4 White Matter


text: NETHERLANDS INSTITUTE OF ECOLOGY

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:NETHERLANDS INSTITUTE OF ECOLOGY

unit: L

characteristic: amount or size of sample collected

text: Photobioreactor

characteristic: broad-scale environmental context

characteristic: collection date

text: Photogranules adapted to experimental conditions

text: Synthetic wastewater

characteristic: environmental medium

text: Netherlands

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Gelderland

characteristic: geographic location (region and locality)

text: Granulated biomass, photogranule

characteristic: local environmental context

text: PCR

characteristic: nucleic acid amplification

text: Qiagen DNAeasy Power Soil Pro

characteristic: nucleic acid extraction

characteristic: number of replicons

text: biofilm metagenome

text: aerobic

characteristic: oxygenation status of sample

characteristic: pH

characteristic: pcr primers

text: Local Treatment of Urban sewage and Streams for Healthy Reuse (LOTUSHR) - www.lotushr.org

characteristic: project name

text: Photobioreactor operated in sequencing batch mode

characteristic: reactor type

text: 15mL of harvested photogranules were homogenised by a glass/Teflon tissue grinder, aliquoted into five 2 mL Eppendor tubes, centrifuged at 14.870 rcf for 10 min and the supernatant discarded, the cell pellets were immediately frozen at -80 �C until further processing.

characteristic: sample material processing

unit: g

characteristic: sample volume or weight for DNA extraction

text: biofilm metagenome

text: Illumnia MiSeq

characteristic: sequencing method

unit: days

characteristic: sludge retention time

text: 16S rRNA gene

characteristic: target gene

text: Photogranules

text: Synthetic wastewater

characteristic: wastewater type


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NETHERLANDS INSTITUTE OF ECOLOGY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: Photobioreactor

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: description

text: Synthetic wastewater

characteristic: environmental medium

text: Netherlands

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Gelderland

text: Granulated biomass, photogranule

text: PCR

text: Qiagen DNAeasy Power Soil Pro

text: biofilm metagenome

characteristic: organism

text: aerobic

text: biofilm metagenome

text: Illumnia MiSeq

text: 18S rRNA gene

text: Photogranules

characteristic: title

text: Synthetic wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NETHERLANDS INSTITUTE OF ECOLOGY

text: public

text: Sequencing batch reactor

text: Aerobic granular sludge adapted to experimental conditions

text: Synthetic wastewater

text: Netherlands

text: Gelderland

characteristic: geographic location (region and locality)

text: Granulated biomass, aerobic granular sludge

characteristic: local environmental context

text: PCR

characteristic: nucleic acid amplification

text: Qiagen DNAeasy Power Soil Pro

characteristic: nucleic acid extraction

characteristic: number of replicons

text: activated sludge metagenome

text: aerobic

characteristic: oxygenation status of sample

characteristic: pH

characteristic: pcr primers

characteristic: project name

text: Sequencing batch reactor

characteristic: reactor type

characteristic: sample material processing

characteristic: sample volume or weight for DNA extraction

text: activated sludge metagenome

text: Illumnia MiSeq

characteristic: sequencing method

characteristic: sludge retention time

text: 16S rRNA gene

characteristic: target gene

text: Aerobic granular sludge

text: Synthetic wastewater

characteristic: wastewater type


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NETHERLANDS INSTITUTE OF ECOLOGY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: Sequencing batch reactor

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: description

text: Synthetic wastewater

characteristic: environmental medium

text: Netherlands

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Gelderland

text: PCR

text: Qiagen DNAeasy Power Soil Pro

text: activated sludge metagenome

characteristic: organism

text: aerobic

text: Sequencing batch reactor

text: activated sludge metagenome

text: Illumnia MiSeq

text: 18S rRNA gene

text: Aerobic granular sludge

characteristic: title

text: Synthetic wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NETHERLANDS INSTITUTE OF ECOLOGY

text: public

text: Photobioreactor

text: Photogranules enriched with polyphosphate accumulating organisms from aerobic granular sludge

text: Synthetic wastewater

text: Netherlands

text: Gelderland

characteristic: geographic location (region and locality)

text: Granulated biomass, photogranule

characteristic: local environmental context

text: PCR

characteristic: nucleic acid amplification

text: Qiagen DNAeasy Power Soil Pro

characteristic: nucleic acid extraction

characteristic: number of replicons

text: biofilm metagenome

text: aerobic

characteristic: oxygenation status of sample

characteristic: pH

characteristic: pcr primers

characteristic: project name

characteristic: reactor type

characteristic: sample material processing

characteristic: sample volume or weight for DNA extraction

text: biofilm metagenome

text: Illumnia MiSeq

characteristic: sequencing method

characteristic: sludge retention time

text: 16S rRNA gene

characteristic: target gene

text: Photogranules

text: Synthetic wastewater

characteristic: wastewater type


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NETHERLANDS INSTITUTE OF ECOLOGY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: Photobioreactor

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: description

text: Synthetic wastewater

characteristic: environmental medium

text: Netherlands

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Gelderland

text: Granulated biomass, photogranule

text: PCR

text: Qiagen DNAeasy Power Soil Pro

text: biofilm metagenome

characteristic: organism

text: aerobic

text: biofilm metagenome

text: Illumnia MiSeq

text: 18S rRNA gene

text: Photogranules

characteristic: title

text: Synthetic wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NETHERLANDS INSTITUTE OF ECOLOGY

text: public

text: Sequencing batch reactor

text: Aerobic granular sludge

text: Synthetic wastewater

text: Netherlands

text: Gelderland

characteristic: geographic location (region and locality)

characteristic: local environmental context

text: PCR

characteristic: nucleic acid amplification

text: Qiagen DNAeasy Power Soil Pro

characteristic: nucleic acid extraction

characteristic: number of replicons

text: activated sludge metagenome

text: aerobic

characteristic: oxygenation status of sample

characteristic: pH

characteristic: pcr primers

characteristic: project name

text: Sequencing batch reactor

characteristic: reactor type

characteristic: sample material processing

characteristic: sample volume or weight for DNA extraction

text: activated sludge metagenome

text: Illumnia MiSeq

characteristic: sequencing method

characteristic: sludge retention time

text: 16S rRNA gene

characteristic: target gene

text: Aerobic granular sludge

text: Synthetic wastewater

characteristic: wastewater type


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NETHERLANDS INSTITUTE OF ECOLOGY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: Photobioreactor

characteristic: broad-scale environmental context

characteristic: collection date

text: Photogranules from previous experiment

characteristic: description

text: Water

characteristic: environmental medium

text: Netherlands

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: The Netherlands Institute of Ecology (NIOO-KNAW), Wageningen, Gelderland

text: Granulated biomass, photogranule

text: PCR

text: Qiagen DNAeasy Power Soil Pro

text: biofilm metagenome

characteristic: organism

text: aerobic

text: LotusHR

text: biofilm metagenome

text: Illumnia MiSeq

text: 16S rRNA gene

text: Photogranules

characteristic: title

text: Synthetic wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NETHERLANDS INSTITUTE OF ECOLOGY

text: public

text: Photobioreactor

text: Photogranules from previous experiment

text: Water

text: Netherlands

characteristic: geographic location (region and locality)

text: Granulated biomass, photogranule

characteristic: local environmental context

text: PCR

characteristic: nucleic acid amplification

text: Qiagen DNAeasy Power Soil Pro

characteristic: nucleic acid extraction

characteristic: number of replicons

text: biofilm metagenome

text: aerobic

characteristic: oxygenation status of sample

characteristic: pH

characteristic: pcr primers

text: LotusHR

characteristic: project name

characteristic: reactor type

characteristic: sample material processing

characteristic: sample volume or weight for DNA extraction

text: biofilm metagenome

text: Illumnia MiSeq

characteristic: sequencing method

characteristic: sludge retention time

text: 18S rRNA gene

characteristic: target gene

text: Photogranules

text: Synthetic wastewater

characteristic: wastewater type


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NETHERLANDS INSTITUTE OF ECOLOGY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: Photobioreactor

characteristic: broad-scale environmental context

characteristic: collection date

text: Photogranules under nitrogen replete conditions

characteristic: description

text: Water

characteristic: environmental medium

text: Netherlands

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Granulated biomass, photogranule

text: PCR

text: Qiagen DNAeasy Power Soil Pro

text: biofilm metagenome

characteristic: organism

text: aerobic

text: LotusHR

text: biofilm metagenome

text: Illumnia MiSeq

text: 16S rRNA gene

text: Photogranules

characteristic: title

text: Synthetic wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NETHERLANDS INSTITUTE OF ECOLOGY

text: public

text: Photobioreactor

text: Water

text: Netherlands

characteristic: geographic location (region and locality)

text: Granulated biomass, photogranule

characteristic: local environmental context

text: PCR

characteristic: nucleic acid amplification

text: Qiagen DNAeasy Power Soil Pro

characteristic: nucleic acid extraction

characteristic: number of replicons

text: biofilm metagenome

text: aerobic

characteristic: oxygenation status of sample

characteristic: pH

characteristic: pcr primers

text: LotusHR

characteristic: project name

characteristic: reactor type

characteristic: sample material processing

characteristic: sample volume or weight for DNA extraction

text: biofilm metagenome

text: Illumnia MiSeq

characteristic: sequencing method

characteristic: sludge retention time

text: 18S rRNA gene

characteristic: target gene

text: Photogranules

text: Synthetic wastewater

characteristic: wastewater type


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NETHERLANDS INSTITUTE OF ECOLOGY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: Photobioreactor

characteristic: broad-scale environmental context

characteristic: collection date

text: Photogranules under nitrogen limiting conditions

characteristic: description

text: Water

characteristic: environmental medium

text: Netherlands

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Granulated biomass, photogranule

text: PCR

text: Qiagen DNAeasy Power Soil Pro

text: biofilm metagenome

characteristic: organism

text: aerobic

text: LotusHR

text: biofilm metagenome

text: Illumnia MiSeq

text: 16S rRNA gene

text: Photogranules

characteristic: title

text: Synthetic wastewater


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NETHERLANDS INSTITUTE OF ECOLOGY

text: public

text: Photobioreactor

text: Water

text: Netherlands

characteristic: geographic location (region and locality)

text: Granulated biomass, photogranule

characteristic: local environmental context

text: PCR

characteristic: nucleic acid amplification

text: Qiagen DNAeasy Power Soil Pro

characteristic: nucleic acid extraction

characteristic: number of replicons

text: biofilm metagenome

text: aerobic

characteristic: oxygenation status of sample

characteristic: pH

characteristic: pcr primers

text: LotusHR

characteristic: project name

characteristic: reactor type

characteristic: sample material processing

characteristic: sample volume or weight for DNA extraction

text: biofilm metagenome

text: Illumnia MiSeq

characteristic: sequencing method

characteristic: sludge retention time

text: 18S rRNA gene

characteristic: target gene

text: Photogranules

text: Synthetic wastewater

characteristic: wastewater type