characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

tag: Namespace:Institut de Genomique Fonctionnelle de Lyon

text: 5 to 8

unit: hour

text: ArrayExpress

text: fruit fly

text: Protocols: For collection of embryos, freshly hatched adult flies of the appropriate genotype were placed in embryo collection vials with standard apple cap plates. Embryos were collected on apple juice agar plates at 25 °C from 5 to 8 hours (after 3 pre-lays of 1 hour), dechorionated using 50% bleach, and washed alternately with water and PBS + 0.1% Triton X-100. The embryos were covalently crosslinked in 1.8% formaldehyde for 15 min, quenched for 5 min with 2M Tris-HCl pH7.5, then crosslinked again with 3 mM DSG for 45min, quenched as above and stored at -80°C. Nucleic acid was extracted as per the Micro-C protocol in Hsieh et al., 2020, with appropriate modifications for Drosophila embryos. Nuclear extraction was carried out as described in Ghavi-Helm et al., 2014 using cold MB#1 buffer (50 mM NaCl, 10 mM Tris-HCl pH 7.5, 5 mM MgCl2, 1 mM CaCl2, freshly added 0.2% NP-40 and 1x PIC) to resuspend the embryos and extract the nuclei by Dounce homogenization. Nuclei from each sample (~600ng) was then treated with an appropriate concentration of Micrococcal Nuclease (~45U) to yield a 90% mononucleosome / 10% dinucleosome ratio. Micro-C libraries were constructed based on Hsieh et al., 2020, with appropriate modifications for Drosophila embryos. For each sample, chromatin obtained from 7 MNase reactions were combined and taken forward for end-chewing, end-labelling, and proximity ligation. After reverse cross-linking, 150-200 ng of the obtained chromatin was size-selected for ligated mononucleosomes (250-400 bp) on a 3.5% NuSieve agarose gel. The size-selected chromatin was then pulled-down with 25 µl of streptavidin beads, and taken forward for library preparation using the NEBNext Ultra II DNA Library Prep Kit for Illumina.

characteristic: description

text: embryonic stage 10 to 11

text: twiΔE3, E3(+51kb)

text: mix pool of embryos

characteristic: individual

text: not applicable

text: Drosophila melanogaster

text: Drosophila melanogaster

text: whole organism

text: Drosophila melanogaster

text: mixed sex

text: twiΔE3, E3(+51kb)


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

text: Institut de Genomique Fonctionnelle de Lyon

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 5 to 8

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: embryonic stage 10 to 11

characteristic: developmental stage

text: twiΔE3, E3(+51kb)

characteristic: genotype

text: mix pool of embryos

text: not applicable

characteristic: isolate

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: whole organism

characteristic: organism part

text: Drosophila melanogaster

text: mixed sex

characteristic: sex

text: twiΔE3, E3(+51kb)

characteristic: strain

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: 5 to 8

text: ArrayExpress

text: fruit fly

characteristic: description

text: embryonic stage 10 to 11

text: y[1] w[1118]

text: mix pool of embryos

text: not applicable

text: Drosophila melanogaster

text: Drosophila melanogaster

text: whole organism

text: Drosophila melanogaster

text: mixed sex

text: wild type


characteristic: ENA-CHECKLIST

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 2 to 5

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: Protocols: Freshly hatched adults of the appropriate genotype were placed in embryo collection vials with standard apple cap plates. Drosophila embryos were collected on apple juice agar plates at 25 °C at the appropriate time-point (after 3 pre-lays of 1 hour for stage-specific collections), dechorionated using 50% bleach, and washed alternately with water and PBS + 0.1% Triton X-100. The embryos were covalently crosslinked in 1.8% formaldehyde for 15 min at room temperature and stored at -80°C. About 100 to 1000 frozen embryos were homogenized in 10 ml of chilled cell lysis buffer (10mM Tris-HCl, pH8; 10 m m NaCl; 0.2% IGEPAL + Complete) on ice. The lysate was incubated 15 min on ice, and spun at 3,000g for 5 min to pellet the nuclei. The nuclei were then washed in cell lysis buffer, spun at 3,000g for 5 min and resuspended in 50 µl of 0.3% SDS before 10 min incubation at 37C. 148µl of water and 25µl of 10% Triton X-100 were added to the nuclei before incubating for an additional 15 min at 37C. 25µl of 10x CutSmart buffer and 25U of the MboI restriction enzyme were added, and the nuclei were incubated for 90 min at 37C, shaking gently. Another 25U of the MboI were added and the nuclei incubated for another 90 min. The nuclei were then incubated for 20 min at 62C to inactivate the enzyme. The following components were added before incubating for 2 hours at 25C with gently shaking: 556µl of water, 100µl of 10x ligation buffer, 80µ of 10% Triton, 12µl of 10mg/ml BSA, and 2000U of T4 DNA ligase. Another 2000U of T4 DNA ligase was added and the nuclei incubated for another 2 hours. The nuclei were spun at 2500g for 5min and the pellet ressuspended in elution buffer (10mM Tris-HCl, pH8; 1 mM EDTA; 0.5M NaCl; 1% SDS solution; 2µg/µl proteinase K) and incubated 30 min at 55C then overnight at 65C. The next day, 100μg of RNaseA were added to the reaction before 15min incubation at 37C. The DNA was purified using the Zymo ChIP DNA Clean & Concentrator kit and elute in 50 µL of 10 mM Tris-Cl pH 8.0. The DNA was then digested with NlaIII overnight at 37C. The next day, the sample was incubated for 20 min at 65C to inactivate the enzyme. The second liation was carried out using 200U of T4 DNA ligase for 4 hours at 16C then 30 min at room temperature. The sample was purified using Nucleomag PCR beads and eluted in 300µl of 10 mM Tris-HCl pH 8.0. The DNA was amplified from 320 ng of 4C template for 2 cycles using the Q5 Hot Start DNA Polymerase. The PCR product was purified using SPRIselect beads (Beckman Coulter) and 100 ng of each PCR product was used to generate the final libraries using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs). The libraries were indexed for multiplexing using NEBNext multiplex oligos kit for Illumina (New England Biolabs).

text: embryonic stage 5 to 9

text: E3(-1.6 Mb)

text: pool of 100 to 1000 embryos

characteristic: individual

text: not applicable

text: Drosophila melanogaster

text: Drosophila melanogaster

text: whole embryo

characteristic: replicate

text: Drosophila melanogaster

text: mixed

text: E3(-1.6 Mb)

characteristic: viewpoint


text: public

text: 5 to 8

text: ArrayExpress

text: fruit fly

characteristic: common name

text: embryonic stage 10 to 11

characteristic: developmental stage

text: E3(-1.6 Mb)

characteristic: genotype

text: pool of 100 to 1000 embryos

text: not applicable

characteristic: isolate

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: whole embryo

characteristic: organism part

text: Drosophila melanogaster

text: mixed

characteristic: sex

text: E3(-1.6 Mb)

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: 2 to 5

text: ArrayExpress

text: fruit fly

characteristic: description

text: embryonic stage 5 to 9

text: pool of 100 to 1000 embryos

text: not applicable

text: Drosophila melanogaster

text: Drosophila melanogaster

text: whole embryo

text: Drosophila melanogaster

text: mixed


text: public

characteristic: SRA accession

characteristic: Submitter Id

text: 5 to 8

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: embryonic stage 10 to 11

characteristic: developmental stage

text: pool of 100 to 1000 embryos

characteristic: individual

text: not applicable

text: Drosophila melanogaster

text: Drosophila melanogaster

text: whole embryo

characteristic: replicate

text: Drosophila melanogaster

text: mixed

characteristic: viewpoint


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: 2 to 5

text: ArrayExpress

text: fruit fly

text: embryonic stage 5 to 9

text: twiΔE3, E3(+51kb)

characteristic: genotype

text: pool of 100 to 1000 embryos

text: not applicable

characteristic: isolate

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: whole embryo

characteristic: organism part

text: Drosophila melanogaster

text: mixed

characteristic: sex

text: twiΔE3, E3(+51kb)

characteristic: strain

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 5 to 8

text: ArrayExpress

text: fruit fly

characteristic: description

text: embryonic stage 10 to 11

text: twiΔE3, E3(+51kb)

text: pool of 100 to 1000 embryos

text: not applicable

text: Drosophila melanogaster

text: Drosophila melanogaster

text: whole embryo

text: Drosophila melanogaster

text: mixed

text: twiΔE3, E3(+51kb)


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: 2 to 5

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: embryonic stage 5 to 9

characteristic: developmental stage

text: twiΔE3, E3(+7.5kb)

text: pool of 100 to 1000 embryos

characteristic: individual

text: not applicable

text: Drosophila melanogaster

text: Drosophila melanogaster

text: whole embryo

characteristic: replicate

text: Drosophila melanogaster

text: mixed

text: twiΔE3, E3(+7.5kb)

characteristic: viewpoint


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: 5 to 8

text: ArrayExpress

text: fruit fly

text: embryonic stage 10 to 11

text: twiΔE3, E3(+7.5kb)

characteristic: genotype

text: pool of 100 to 1000 embryos

text: not applicable

characteristic: isolate

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: whole embryo

characteristic: organism part

text: Drosophila melanogaster

text: mixed

characteristic: sex

text: twiΔE3, E3(+7.5kb)

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 5 to 8

text: ArrayExpress

text: fruit fly

characteristic: description

text: embryonic stage 10 to 11

text: y[1] w[1118]

text: pool of 100 to 1000 embryos

text: not applicable

text: Drosophila melanogaster

text: Drosophila melanogaster

text: whole embryo

text: Drosophila melanogaster

text: mixed

text: wild type


text: Plasmodium falciparum

characteristic: ArrayExpress-SPECIES

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: malaria parasite P. falciparum

characteristic: common name

text: Plasmodium falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Plasmodium malariae

characteristic: organism

text: Plasmodium malariae

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Plasmodium fragile

text: Plasmodium fragile

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Plasmodium simium

text: Plasmodium simium

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Plasmodium berghei yoelii

text: Plasmodium berghei yoelii

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Plasmodium yoelii

text: Plasmodium yoelii

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Plasmodium vinckei

text: Plasmodium vinckei

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Babesia bovis

text: Babesia bovis

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Babesia canis

text: Babesia canis

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Babesia bigemina

text: Babesia bigemina

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Babesia microti

text: Babesia microti

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Babesia rodhaini

text: Babesia rodhaini

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Theileria equi

text: Theileria equi

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Babesia caballi

text: Babesia caballi

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Theileria annulata

text: Theileria annulata

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Theileria parva

text: Theileria parva

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Theileria buffeli

text: Theileria buffeli

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Theileria sergenti

text: Theileria sergenti

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Babesia ovis

text: Babesia ovis

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Paramecium caudatum

text: Paramecium caudatum

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Glaucoma chattoni

text: Glaucoma chattoni

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Paramecium primaurelia

text: Paramecium primaurelia

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Paramecium aurelia

text: Paramecium aurelia

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Paramecium tetraurelia

text: Paramecium tetraurelia

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Tetrahymena americanis

text: Tetrahymena americanis

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Tetrahymena australis

text: Tetrahymena australis

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Tetrahymena borealis

text: Tetrahymena borealis

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Tetrahymena canadensis

text: Tetrahymena canadensis

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Tetrahymena capricornis

text: Tetrahymena capricornis

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Tetrahymena caudata

text: Tetrahymena caudata

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Tetrahymena elliotti

text: Tetrahymena elliotti

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Tetrahymena furgasoni

text: Tetrahymena furgasoni

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Tetrahymena hyperangularis

text: Tetrahymena hyperangularis

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Tetrahymena malaccensis

text: Tetrahymena malaccensis

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Tetrahymena mimbres

text: Tetrahymena mimbres

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Tetrahymena nanneyi

text: Tetrahymena nanneyi

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Tetrahymena patula

text: Tetrahymena patula

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Tetrahymena leucophrys

text: Tetrahymena leucophrys

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Tetrahymena pigmentosa

text: Tetrahymena pigmentosa

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Tetrahymena pyriformis

text: Tetrahymena pyriformis

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Tetrahymena paravorax

text: Tetrahymena paravorax

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Plasmodium falciparum RO-33

text: Plasmodium falciparum RO-33

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Plasmodium falciparum CAMP/Malaysia

text: Plasmodium falciparum CAMP/Malaysia

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Plasmodium falciparum CDC/Honduras

text: Plasmodium falciparum CDC/Honduras

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Plasmodium falciparum FC27/Papua New Guinea

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Plasmodium falciparum FCR-3/Gambia

text: Plasmodium falciparum FCR-3/Gambia

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Plasmodium falciparum Mad20/Papua New Guinea

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Plasmodium falciparum K1

text: Plasmodium falciparum K1

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Plasmodium falciparum NF54

text: Plasmodium falciparum NF54

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Plasmodium falciparum T4/Thailand

text: Plasmodium falciparum T4/Thailand

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Plasmodium falciparum FCM17/Senegal

text: Plasmodium falciparum FCM17/Senegal

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Plasmodium falciparum LE5

text: Plasmodium falciparum LE5

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Plasmodium falciparum V1

text: Plasmodium falciparum V1

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Plasmodium falciparum NF7/Ghana

text: Plasmodium falciparum NF7/Ghana

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Plasmodium gallinaceum

text: Plasmodium gallinaceum

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Plasmodium knowlesi

text: Plasmodium knowlesi

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Plasmodium falciparum isolate WELLCOME

text: Plasmodium falciparum isolate WELLCOME

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Plasmodium knowlesi strain H

text: Plasmodium knowlesi strain H

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Plasmodium lophurae

text: Plasmodium lophurae

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Plasmodium knowlesi strain Nuri

text: Plasmodium knowlesi strain Nuri

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: malaria parasite P. vivax

text: Plasmodium vivax

text: Plasmodium vivax

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Plasmodium reichenowi

text: Plasmodium reichenowi

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Tetrahymena sonneborni

text: Tetrahymena sonneborni

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Tetrahymena tropicalis

text: Tetrahymena tropicalis

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Tetrahymena rostrata

text: Tetrahymena rostrata

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Tetrahymena thermophila

text: Tetrahymena thermophila

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Tetrahymena asiatica

text: Tetrahymena asiatica

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Tetrahymena corlissi

text: Tetrahymena corlissi

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Tetrahymena limacis

text: Tetrahymena limacis

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Tetrahymena silvana

text: Tetrahymena silvana

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Tetrahymena vorax

text: Tetrahymena vorax

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Tetrahymena setosa

text: Tetrahymena setosa

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Tetrahymena nipissingi

text: Tetrahymena nipissingi

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Tetrahymena cosmopolitanis

text: Tetrahymena cosmopolitanis

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Tetrahymena hegewischi

text: Tetrahymena hegewischi

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Dexiostoma campyla

text: Dexiostoma campyla

text: Plasmodium falciparum


text: public

tag: Namespace:University College London, Great Ormond Street Institute of Child Health

text: ArrayExpress

characteristic: broker name

text: retinal pigment epithelial cell

text: human

text: Protocols: iPSC-derived retinal pigment epithelium samples were collected in 1.5mL eppendorf tube and snap frozen on dry ice before stored at -80 degree until use Total RNA was extracted using miRNeasy micro kit (Qiagen, 217084) Libraries were prepared using KAPA mRNA HyperPrep Kit (Roche, KK8580) according to manufacturer’s instructions, Quality of libraries was tested using High Sensitivity Agilent DNA 1000 assay (Agilent, 5067-4626)) on Agilent TapeStation 4200

text: Usher syndrome type 1

characteristic: disease

text: MYO7A mutation

characteristic: genotype

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: passage 21

characteristic: passage

text: induced pluripotent stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: female

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: University College London, Great Ormond Street Institute of Child Health

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: retinal pigment epithelial cell

text: human

text: Usher syndrome type 1

text: MYO7A mutation

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: passage 22

text: induced pluripotent stem cell

text: Homo sapiens

text: female

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: retinal pigment epithelial cell

text: human

characteristic: common name

characteristic: description

text: Usher syndrome type 1

text: MYO7A mutation

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: passage 24

text: induced pluripotent stem cell

text: Homo sapiens

text: female


text: public

text: ArrayExpress

characteristic: broker name

text: retinal pigment epithelial cell

text: human

text: Usher syndrome type 1

characteristic: disease

text: MYO7A mutation

characteristic: genotype

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: passage 28

text: induced pluripotent stem cell

text: Homo sapiens

text: female


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

text: public

text: ArrayExpress

text: retinal pigment epithelial cell

text: human

text: Usher syndrome type 1

text: MYO7A mutation

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: passage 39

characteristic: passage

text: induced pluripotent stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: female

characteristic: sex


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: retinal pigment epithelial cell

text: human

text: Usher syndrome type 1

text: MYO7A mutation

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: passage 41

text: induced pluripotent stem cell

text: Homo sapiens

text: female

characteristic: title


text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: retinal pigment epithelial cell

text: human

characteristic: common name

characteristic: description

text: normal

characteristic: disease

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: passage 38

text: induced pluripotent stem cell

text: Homo sapiens

text: female


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: ArrayExpress

text: retinal pigment epithelial cell

text: human

text: normal

text: wild type genotype

characteristic: genotype

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: passage 9

text: induced pluripotent stem cell

text: Homo sapiens

text: male


text: Plasmodium falciparum

characteristic: ArrayExpress-SPECIES

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum


E-MTAB-12187:Sample 2

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12187:Sample 2

tag: Namespace:Gynecologic Cancer Center, Department of Radiation Oncology, The Ajou University of School of Medicine, Suwon 16499, Korea

unit: year

characteristic: age

text: ArrayExpress

characteristic: clinical information

text: human

text: Protocols: Exosome was isolated from human plasma by mixing the plasma with Exo2D RNA solution (Exosomeplus). Exosomal RNA from plasma-derived exosomes was extracted using the miRNeasy Serum/Plasma Kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions.The extracted RNA concentration was calculated by Quant-IT RiboGreen(Invitrogen). RNA size was confirmed using Agilent RNA 6000 Pico Kit and Small RNA Kit on Agilent 2100 Bioanalyzer (Agilent Technologies, Böblingen, Germany). The 10ng of RNA isolated from each sample was used to construct sequencing libraries with the SMARTer smRNA-Seq Kit for Illumina, following the manufacturer's protocol. Briefly, Input RNA is first polyadenylated in order to provide a priming sequence for an oligo(dT) primer. cDNA synthesis is primed by the 3’ smRNA dT Primer, which incorporates an adapter sequence at the 5’end of each RNA template, it adds non templated nucleotides which are bound by the SMRT smRNA Oligo-enhanced with locked nucleic acid (LNA) technology for greater sensitivity. In the template-switching step, PrimeScript RT uses the SMART smRNA Oligo as a template for the addition of a second adapter sequence to the 3’end of each first-strand cDNA molecule. In the next step, full-length Illumina adapters (including index sequences for sample multiplexing) are added during PCR amplification. The Forward PCR Primer binds to the sequence added by the SMART smRNA Oligo, while the Reverse PCR Primer binds to the sequence added by the 3’ smRNA dT Primer. The amplified libraries were purified from 6% Novex TBE-PAGE gels (Thermo Fisher, MA) to excise over than 138 bp (over than 18 bp of cDNA plus 120 bp of adaptors) fractionResulting library cDNA molecules include sequences required for clustering on an Illumina flow cell.

text: adult

characteristic: developmental stage

text: cervical cancer

characteristic: disease staging

text: exosome

characteristic: fraction

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: blood plasma

characteristic: organism part

text: Homo sapiens

text: female

characteristic: sex

text: Sample 2

characteristic: title


E-MTAB-12187:Sample 4

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Gynecologic Cancer Center, Department of Radiation Oncology, The Ajou University of School of Medicine, Suwon 16499, Korea

text: public

characteristic: SRA accession

text: E-MTAB-12187:Sample 4

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

characteristic: description

text: adult

text: cervical cancer

characteristic: disease

text: exosome

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: female

text: Sample 4


E-MTAB-12187:Sample 6

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12187:Sample 6

characteristic: age

text: ArrayExpress

characteristic: clinical information

text: human

text: adult

text: cervical cancer

text: exosome

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: female

text: Sample 6


E-MTAB-12187:Sample 8

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: SRA accession

text: E-MTAB-12187:Sample 8

text: ArrayExpress

text: human

text: adult

characteristic: developmental stage

text: cervical cancer

characteristic: disease staging

text: exosome

characteristic: fraction

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: blood plasma

characteristic: organism part

text: Homo sapiens

text: female

characteristic: sex

text: Sample 8

characteristic: title


E-MTAB-12187:Sample 10

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-12187:Sample 10

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

characteristic: description

text: adult

text: cervical cancer

characteristic: disease

text: exosome

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: female

text: Sample 10


E-MTAB-12187:Sample 12

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12187:Sample 12

characteristic: age

text: ArrayExpress

characteristic: clinical information

text: human

text: adult

text: cervical cancer

text: exosome

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: female

text: Sample 12


E-MTAB-12187:Sample 14

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-12187:Sample 14

text: ArrayExpress

text: human

text: adult

characteristic: developmental stage

text: cervical cancer

characteristic: disease staging

text: exosome

characteristic: fraction

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: blood plasma

characteristic: organism part

text: Homo sapiens

text: female

characteristic: sex

text: Sample 14

characteristic: title


E-MTAB-12187:Sample 16

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-12187:Sample 16

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

characteristic: description

text: adult

text: cervical cancer

characteristic: disease

text: exosome

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: female

text: Sample 16


E-MTAB-12187:Sample 18

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12187:Sample 18

characteristic: age

text: ArrayExpress

characteristic: clinical information

text: human

text: adult

text: cervical cancer

text: exosome

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: female

text: Sample 18


E-MTAB-12187:Sample 20

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: SRA accession

text: E-MTAB-12187:Sample 20

text: ArrayExpress

text: human

text: adult

characteristic: developmental stage

text: cervical cancer

characteristic: disease staging

text: exosome

characteristic: fraction

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: blood plasma

characteristic: organism part

text: Homo sapiens

text: female

characteristic: sex

text: Sample 20

characteristic: title


E-MTAB-12187:Sample 22

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-12187:Sample 22

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

characteristic: description

text: adult

text: cervical cancer

characteristic: disease

text: exosome

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: female

text: Sample 22


E-MTAB-12187:Sample 24

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12187:Sample 24

characteristic: age

text: ArrayExpress

characteristic: clinical information

text: human

text: adult

text: cervical cancer

text: exosome

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: female

text: Sample 24


E-MTAB-12187:Sample 1

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-12187:Sample 1

text: ArrayExpress

text: human

text: adult

characteristic: developmental stage

text: cervical cancer

characteristic: disease staging

text: exosome

characteristic: fraction

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: blood plasma

characteristic: organism part

text: Homo sapiens

text: female

characteristic: sex

text: Sample 1

characteristic: title


E-MTAB-12187:Sample 3

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-12187:Sample 3

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

characteristic: description

text: adult

text: cervical cancer

characteristic: disease

text: exosome

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: female

text: Sample 3


E-MTAB-12187:Sample 5

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12187:Sample 5

characteristic: age

text: ArrayExpress

characteristic: clinical information

text: human

text: adult

text: cervical cancer

text: exosome

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: female

text: Sample 5


E-MTAB-12187:Sample 7

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: SRA accession

text: E-MTAB-12187:Sample 7

text: ArrayExpress

text: human

text: adult

characteristic: developmental stage

text: cervical cancer

characteristic: disease staging

text: exosome

characteristic: fraction

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: blood plasma

characteristic: organism part

text: Homo sapiens

text: female

characteristic: sex

text: Sample 7

characteristic: title


E-MTAB-12187:Sample 9

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-12187:Sample 9

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

characteristic: description

text: adult

text: cervical cancer

characteristic: disease

text: exosome

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: female

text: Sample 9


E-MTAB-12187:Sample 11

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12187:Sample 11

characteristic: age

text: ArrayExpress

characteristic: clinical information

text: human

text: adult

text: cervical cancer

text: exosome

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: female

text: Sample 11


E-MTAB-12187:Sample 13

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-12187:Sample 13

text: ArrayExpress

text: human

text: adult

characteristic: developmental stage

text: cervical cancer

characteristic: disease staging

text: exosome

characteristic: fraction

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: blood plasma

characteristic: organism part

text: Homo sapiens

text: female

characteristic: sex

text: Sample 13

characteristic: title


E-MTAB-12187:Sample 15

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-12187:Sample 15

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

characteristic: description

text: adult

text: cervical cancer

characteristic: disease

text: exosome

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: female

text: Sample 15


E-MTAB-12187:Sample 17

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12187:Sample 17

characteristic: age

text: ArrayExpress

characteristic: clinical information

text: human

text: adult

text: cervical cancer

text: exosome

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: female

text: Sample 17


E-MTAB-12187:Sample 19

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: SRA accession

text: E-MTAB-12187:Sample 19

text: ArrayExpress

text: human

text: adult

characteristic: developmental stage

text: cervical cancer

characteristic: disease staging

text: exosome

characteristic: fraction

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: blood plasma

characteristic: organism part

text: Homo sapiens

text: female

characteristic: sex

text: Sample 19

characteristic: title


E-MTAB-12187:Sample 21

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-12187:Sample 21

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

characteristic: description

text: adult

text: cervical cancer

characteristic: disease

text: exosome

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: female

text: Sample 21


E-MTAB-12187:Sample 23

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12187:Sample 23

characteristic: age

text: ArrayExpress

text: early progression

characteristic: clinical information

text: human

text: adult

text: cervical cancer

text: exosome

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: female

text: Sample 23


E-MTAB-12194:Sample 1

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

text: public

characteristic: SRA accession

text: E-MTAB-12194:Sample 1

tag: Namespace:UNIVERSITY OF MANCHESTER

text: ArrayExpress

text: house mouse

text: Protocols: Female, 8–12-week-old C57BL/6 mice were purchased from ENVIGO and housed in the Biological Services Facility of The University of Manchester on a 12/12-hour light/dark cycle. Mice were given unlimited access to food (Bekay, B&K Universal, Hull, UK) and water. All procedures were approved by the institution's review board and performed under relevant Home Office licenses according to the UK Animals (Scientific Procedures) Act, 1986. YUMM2.1 cells (7 × 106 cells in 100 µL) were pre-treated with IFNγ cell culture for 2 weeks (maintained in RPMI-1640 (Sigma-Aldrich) medium supplemented with 10% fetal bovine serum (FBS; Life Technologies), 1% penicillin-streptomycin (P/S; Sigma-Aldrich) and with 50 IU/ml, replacing medium and IFNγ every 72 h with passaging of cells as necessary). These pretreated cells were subcutaneously injected into the left flank of mice under isoflurane anaesthesia. Tumour size (determined by calliper measurements) and mouse weight were monitored 3 times per week (every 2-3 days). When tumours reached approximately 150 mm3, mice were randomised into either α-PD-1 group or α-PD-1 + PARP14i group. Both groups of mice were administered with 2 doses of 300 µg of α-PD-1 antibody (BioXCell) via intraperitoneal (i.p.) injection (1 dose every 3 days). Mice were administered concurrently 14 doses vehicle or 500 mg/kg of RBN012759 by oral gavage (BID) (2 doses daily). RBN012759 was dissolved in the vehicle 0.5% methylcellulose (Sigma-Aldrich) + 0.2% Tween 80 (Sigma-Aldrich). Each dose was delivered in a volume of 0.2 mL/20 g mouse (10 mL/kg) and adjusted for the last recorded weight of individual animals. 24 h following the last dose of RBN012759, mice were euthanized and tumours dissected. Female, 8–12-week-old C57BL/6 mice were purchased from ENVIGO and housed in the Biological Services Facility of The University of Manchester on a 12/12-hour light/dark cycle. Mice were given unlimited access to food (Bekay, B&K Universal, Hull, UK) and water. All procedures were approved by the institution's review board and performed under relevant Home Office licenses according to the UK Animals (Scientific Procedures) Act, 1986. YUMM2.1 cells (7 × 106 cells in 100 µL) were pre-treated with IFNγ cell culture for 2 weeks (maintained in RPMI-1640 (Sigma-Aldrich) medium supplemented with 10% fetal bovine serum (FBS; Life Technologies), 1% penicillin-streptomycin (P/S; Sigma-Aldrich) and with 50 IU/ml, replacing medium and IFNγ every 72 h with passaging of cells as necessary). These pretreated cells were subcutaneously injected into the left flank of mice under isoflurane anaesthesia. Tumour size (determined by calliper measurements) and mouse weight were monitored 3 times per week (every 2-3 days). When tumours reached approximately 150 mm3, mice were randomised into either α-PD-1 group or α-PD-1 + PARP14i group. Both groups of mice were administered with 2 doses of 300 µg of α-PD-1 antibody (BioXCell) via intraperitoneal (i.p.) injection (1 dose every 3 days). Mice were administered concurrently 14 doses vehicle or 500 mg/kg of RBN012759 by oral gavage (BID) (2 doses daily). RBN012759 was dissolved in the vehicle 0.5% methylcellulose (Sigma-Aldrich) + 0.2% Tween 80 (Sigma-Aldrich). Each dose was delivered in a volume of 0.2 mL/20 g mouse (10 mL/kg) and adjusted for the last recorded weight of individual animals. 24 h following the last dose of RBN012759, mice were euthanized and tumours dissected. Female, 8–12-week-old C57BL/6 mice were purchased from ENVIGO and housed in the Biological Services Facility of The University of Manchester on a 12/12-hour light/dark cycle. Mice were given unlimited access to food (Bekay, B&K Universal, Hull, UK) and water. All procedures were approved by the institution's review board and performed under relevant Home Office licenses according to the UK Animals (Scientific Procedures) Act, 1986. YUMM2.1 cells (7 × 106 cells in 100 µL) were pre-treated with IFNγ cell culture for 2 weeks (maintained in RPMI-1640 (Sigma-Aldrich) medium supplemented with 10% fetal bovine serum (FBS; Life Technologies), 1% penicillin-streptomycin (P/S; Sigma-Aldrich) and with 50 IU/ml, replacing medium and IFNγ every 72 h with passaging of cells as necessary). These pretreated cells were subcutaneously injected into the left flank of mice under isoflurane anaesthesia. Tumour size (determined by calliper measurements) and mouse weight were monitored 3 times per week (every 2-3 days). When tumours reached approximately 150 mm3, mice were randomised into either α-PD-1 group or α-PD-1 + PARP14i group. Both groups of mice were administered with 2 doses of 300 µg of α-PD-1 antibody (BioXCell) via intraperitoneal (i.p.) injection (1 dose every 3 days). Mice were administered concurrently 14 doses vehicle or 500 mg/kg of RBN012759 by oral gavage (BID) (2 doses daily). RBN012759 was dissolved in the vehicle 0.5% methylcellulose (Sigma-Aldrich) + 0.2% Tween 80 (Sigma-Aldrich). Each dose was delivered in a volume of 0.2 mL/20 g mouse (10 mL/kg) and adjusted for the last recorded weight of individual animals. 24 h following the last dose of RBN012759, mice were euthanized and tumours dissected. Total RNA was isolated using the RNAeasy mini kit. RNA integrity was assessed on the Agilent 2200 TapeStation (Agilent Technologies). TruSeq Stranded mRNA assay (Illumina)

text: adult

characteristic: developmental stage

text: melanoma

text: tumour in vivo

characteristic: growth condition

text: not applicable

text: Mus musculus

text: Mus musculus

text: skin

characteristic: organism part

text: Mus musculus

text: interferon gamma

characteristic: stimulus

text: Sample 1

characteristic: title

text: α-PD-1 antibody

characteristic: treatment

text: injected in C57BL/6 mice

characteristic: xenograft


E-MTAB-12194:Sample 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

text: public

text: E-MTAB-12194:Sample 2

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: melanoma

characteristic: disease

text: tumour in vivo

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: interferon gamma

text: Sample 2

text: α-PD-1 antibody

text: injected in C57BL/6 mice


E-MTAB-12194:Sample 3

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12194:Sample 3

text: ArrayExpress

text: house mouse

text: adult

text: melanoma

text: tumour in vivo

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: skin

text: Mus musculus

text: interferon gamma

text: Sample 3

text: α-PD-1 antibody

text: injected in C57BL/6 mice


E-MTAB-12194:Sample 4

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12194:Sample 4

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: melanoma

text: tumour in vivo

characteristic: growth condition

text: not applicable

text: Mus musculus

text: Mus musculus

text: skin

characteristic: organism part

text: Mus musculus

text: interferon gamma

characteristic: stimulus

text: Sample 4

characteristic: title

text: α-PD-1 antibody

characteristic: treatment

text: injected in C57BL/6 mice

characteristic: xenograft


E-MTAB-12194:Sample 5

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

text: public

text: E-MTAB-12194:Sample 5

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: melanoma

text: tumour in vivo

text: not applicable

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: interferon gamma

text: Sample 5

text: α-PD-1 antibody

text: injected in C57BL/6 mice


E-MTAB-12194:Sample 6

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12194:Sample 6

text: ArrayExpress

text: house mouse

text: adult

text: melanoma

characteristic: disease

text: tumour in vivo

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: interferon gamma

text: Sample 6

text: α-PD-1 antibody

text: injected in C57BL/6 mice


E-MTAB-12194:Sample 7

text: UNIVERSITY OF MANCHESTER

text: public

characteristic: SRA accession

text: E-MTAB-12194:Sample 7

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: melanoma

text: tumour in vivo

characteristic: growth condition

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: skin

characteristic: organism part

text: Mus musculus

text: interferon gamma

characteristic: stimulus

text: Sample 7

characteristic: title

text: α-PD-1 antibody

text: injected in C57BL/6 mice


E-MTAB-12194:Sample 8

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

text: public

text: E-MTAB-12194:Sample 8

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

text: adult

text: melanoma

text: tumour in vivo

text: not applicable

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: interferon gamma

text: Sample 8

text: α-PD-1 antibody

characteristic: treatment

text: injected in C57BL/6 mice

characteristic: xenograft


E-MTAB-12195:Sample 1

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12195:Sample 1

text: ArrayExpress

text: human

characteristic: common name

text: Protocols: This submission studied the inhibition of PARP14 under IFNγ stimulation in three cell lines. A375 (https://www.cellosaurus.org/CVCL_0132) human melanoma cell line was maintained in DMEM (high glucose) medium. MC38 (https://www.cellosaurus.org/CVCL_B288) mouse colon cancer cell line was maintained in RPMI-1640 (Sigma-Aldrich) medium. YUMM2.1 (https://www.cellosaurus.org/CVCL_JK30) mouse melanoma cell line was maintained in RPMI-1640 (Sigma-Aldrich) medium. All growth media were supplemented with 10% fetal bovine serum (FBS; Life Technologies), 1% penicillin-streptomycin (P/S; Sigma-Aldrich) and 0.1% by volume of DMSO (solvent for the inhibitors). All cells were maintained under standard conditions at 37°C in a 5% CO2 humidified incubator and passaged before reaching confluency. Cell cultures were routinely tested for mycoplasma contamination by PCR. For chronic IFNγ stimulation, human cells (A375) were treated with 20 IU/ml, mouse cells (YUMM2.1 and MC38) with 50 IU/ml, replacing medium and IFNγ every 72 h with passaging of cells as necessary for 2 weeks. PARP14 inhibition was with 100 nM RBN012759 or 500 nM RBN012811 for the last 48 h. Recombinant human IFNγ (PHC4031) and recombinant mouse IFNγ (PMC4031) were purchased from Gibco and used at the indicated concentrations. The PARP14 inhibitors RBN012759 and RBN012811 were kindly provided by Ribon Therapeutics and used at the indicated concentrations. This submission studied the inhibition of PARP14 under IFNγ stimulation in three cell lines. A375 (https://www.cellosaurus.org/CVCL_0132) human melanoma cell line was maintained in DMEM (high glucose) medium. MC38 (https://www.cellosaurus.org/CVCL_B288) mouse colon cancer cell line was maintained in RPMI-1640 (Sigma-Aldrich) medium. YUMM2.1 (https://www.cellosaurus.org/CVCL_JK30) mouse melanoma cell line was maintained in RPMI-1640 (Sigma-Aldrich) medium. All growth media were supplemented with 10% fetal bovine serum (FBS; Life Technologies), 1% penicillin-streptomycin (P/S; Sigma-Aldrich) and 0.1% by volume of DMSO (solvent for the inhibitors). All cells were maintained under standard conditions at 37°C in a 5% CO2 humidified incubator and passaged before reaching confluency. Cell cultures were routinely tested for mycoplasma contamination by PCR. For chronic IFNγ stimulation, human cells (A375) were treated with 20 IU/ml, mouse cells (YUMM2.1 and MC38) with 50 IU/ml, replacing medium and IFNγ every 72 h with passaging of cells as necessary for 2 weeks. PARP14 inhibition was with 100 nM RBN012759 or 500 nM RBN012811 for the last 48 h. Recombinant human IFNγ (PHC4031) and recombinant mouse IFNγ (PMC4031) were purchased from Gibco and used at the indicated concentrations. The PARP14 inhibitors RBN012759 and RBN012811 were kindly provided by Ribon Therapeutics and used at the indicated concentrations. Total RNA was isolated using the RNAeasy mini kit. RNA integrity was assessed on the Agilent 2200 TapeStation (Agilent Technologies). TruSeq Stranded mRNA assay (Illumina)

characteristic: description

text: melanoma

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin

text: Homo sapiens

text: interferon gamma

text: Sample 1


E-MTAB-12195:Sample 2

text: UNIVERSITY OF MANCHESTER

text: public

characteristic: SRA accession

text: E-MTAB-12195:Sample 2

text: ArrayExpress

text: human

text: melanoma

characteristic: disease

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: skin

characteristic: organism part

text: Homo sapiens

text: interferon gamma

text: Sample 2


E-MTAB-12195:Sample 3

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

text: public

text: E-MTAB-12195:Sample 3

text: ArrayExpress

text: human

text: melanoma

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin

text: Homo sapiens

text: interferon gamma

characteristic: stimulus

text: Sample 3

characteristic: title


E-MTAB-12195:Sample 4

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12195:Sample 4

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: human

text: melanoma

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin

text: Homo sapiens

text: interferon gamma

text: Sample 4


E-MTAB-12195:Sample 5

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-12195:Sample 5

text: ArrayExpress

text: human

text: melanoma

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin

text: Homo sapiens

text: interferon gamma

text: Sample 5


E-MTAB-12195:Sample 6

text: UNIVERSITY OF MANCHESTER

text: public

characteristic: INSDC status

text: E-MTAB-12195:Sample 6

text: ArrayExpress

text: human

characteristic: common name

characteristic: description

text: melanoma

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin

text: Homo sapiens

text: interferon gamma

text: Sample 6


E-MTAB-12195:Sample 7

text: UNIVERSITY OF MANCHESTER

text: public

characteristic: SRA accession

text: E-MTAB-12195:Sample 7

text: ArrayExpress

text: human

text: melanoma

characteristic: disease

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: skin

text: Homo sapiens

text: interferon gamma

text: Sample 7


E-MTAB-12195:Sample 8

characteristic: ENA-CHECKLIST

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12195:Sample 8

text: ArrayExpress

text: human

text: melanoma

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: skin

characteristic: organism part

text: Homo sapiens

text: interferon gamma

text: Sample 8


E-MTAB-12195:Sample 9

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

text: public

text: E-MTAB-12195:Sample 9

text: ArrayExpress

text: human

text: melanoma

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin

text: Homo sapiens

text: interferon gamma

characteristic: stimulus

text: Sample 9


E-MTAB-12195:Sample 19

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12195:Sample 19

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: colon adenocarcinoma

text: not applicable

text: Mus musculus

text: Mus musculus

text: colon

text: Mus musculus

text: interferon gamma

text: Sample 19

characteristic: title


E-MTAB-12195:Sample 20

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC first public

text: public

text: E-MTAB-12195:Sample 20

text: ArrayExpress

characteristic: broker name

text: house mouse

text: colon adenocarcinoma

text: not applicable

text: Mus musculus

text: Mus musculus

text: colon

text: Mus musculus

text: interferon gamma

text: Sample 20


E-MTAB-12195:Sample 21

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12195:Sample 21

text: ArrayExpress

text: house mouse

characteristic: common name

text: colon adenocarcinoma

text: not applicable

text: Mus musculus

text: Mus musculus

text: colon

text: Mus musculus

text: interferon gamma

text: Sample 21


E-MTAB-12195:Sample 22

text: UNIVERSITY OF MANCHESTER

text: public

characteristic: SRA accession

text: E-MTAB-12195:Sample 22

text: ArrayExpress

text: house mouse

characteristic: description

text: colon adenocarcinoma

characteristic: disease

text: not applicable

text: Mus musculus

text: Mus musculus

text: colon

text: Mus musculus

text: interferon gamma

text: Sample 22


E-MTAB-12195:Sample 23

characteristic: ENA-CHECKLIST

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12195:Sample 23

text: ArrayExpress

text: house mouse

text: colon adenocarcinoma

text: not applicable

characteristic: isolate

text: Mus musculus

characteristic: organism

text: Mus musculus

text: colon

text: Mus musculus

text: interferon gamma

text: Sample 23


E-MTAB-12195:Sample 24

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

text: public

text: E-MTAB-12195:Sample 24

text: ArrayExpress

text: house mouse

text: colon adenocarcinoma

text: not applicable

text: Mus musculus

text: Mus musculus

text: colon

characteristic: organism part

text: Mus musculus

text: interferon gamma

text: Sample 24


E-MTAB-12195:Sample 25

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12195:Sample 25

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: colon adenocarcinoma

text: not applicable

text: Mus musculus

text: Mus musculus

text: colon

text: Mus musculus

text: interferon gamma

characteristic: stimulus

text: Sample 25


E-MTAB-12195:Sample 26

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC first public

text: public

text: E-MTAB-12195:Sample 26

text: ArrayExpress

text: house mouse

text: colon adenocarcinoma

text: not applicable

text: Mus musculus

text: Mus musculus

text: colon

text: Mus musculus

text: interferon gamma

text: Sample 26

characteristic: title


E-MTAB-12195:Sample 27

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12195:Sample 27

text: ArrayExpress

characteristic: broker name

text: house mouse

text: colon adenocarcinoma

text: not applicable

text: Mus musculus

text: Mus musculus

text: colon

text: Mus musculus

text: interferon gamma

text: Sample 27


E-MTAB-12195:Sample 34

text: UNIVERSITY OF MANCHESTER

text: public

characteristic: SRA accession

text: E-MTAB-12195:Sample 34

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: melanoma

text: not applicable

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: interferon gamma

text: Sample 34


E-MTAB-12195:Sample 35

characteristic: ENA-CHECKLIST

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12195:Sample 35

text: ArrayExpress

text: house mouse

text: melanoma

characteristic: disease

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: interferon gamma

text: Sample 35


E-MTAB-12195:Sample 36

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

text: public

text: E-MTAB-12195:Sample 36

text: ArrayExpress

text: house mouse

text: melanoma

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: skin

text: Mus musculus

text: interferon gamma

text: Sample 36


E-MTAB-12195:Sample 37

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12195:Sample 37

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: melanoma

text: not applicable

text: Mus musculus

text: Mus musculus

text: skin

characteristic: organism part

text: Mus musculus

text: interferon gamma

text: Sample 37


E-MTAB-12195:Sample 38

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC first public

text: public

text: E-MTAB-12195:Sample 38

text: ArrayExpress

text: house mouse

text: melanoma

text: not applicable

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: interferon gamma

characteristic: stimulus

text: Sample 38


E-MTAB-12195:Sample 39

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12195:Sample 39

text: ArrayExpress

text: house mouse

text: melanoma

text: not applicable

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: interferon gamma

text: Sample 39

characteristic: title


E-MTAB-12195:Sample 40

text: UNIVERSITY OF MANCHESTER

text: public

characteristic: SRA accession

text: E-MTAB-12195:Sample 40

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: melanoma

text: not applicable

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: interferon gamma

text: Sample 40


E-MTAB-12195:Sample 41

characteristic: ENA-CHECKLIST

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12195:Sample 41

text: ArrayExpress

text: house mouse

characteristic: description

text: melanoma

characteristic: disease

text: not applicable

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: interferon gamma

text: Sample 41


E-MTAB-12195:Sample 42

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

text: public

text: E-MTAB-12195:Sample 42

text: ArrayExpress

text: house mouse

text: melanoma

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: interferon gamma

text: Sample 42


E-MTAB-12196:Sample 13

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12196:Sample 13

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: Protocols: Growth conditions - This submission studied the effect of IFNγ stimulation on 6 cancer cell lines. A375 (https://www.cellosaurus.org/CVCL_0132) and 501-mel (https://www.cellosaurus.org/CVCL_4633) were human melanoma cell lines. B16-F10 (https://www.cellosaurus.org/CVCL_0159), 5555 (provided by Santiago Zelenay, The University of Manchester) and YUMM2.1 (https://www.cellosaurus.org/CVCL_JK30) mouse melanoma cell lines. MC38 (https://www.cellosaurus.org/CVCL_B288) mouse colon cancer cell line. Human cell lines were maintained in DMEM (high glucose) medium. Mouse cell lines were maintained in RPMI-1640 (Sigma-Aldrich) medium. All growth media were supplemented with 10% fetal bovine serum (FBS; Life Technologies), 1% penicillin-streptomycin (P/S; Sigma-Aldrich). All cells were maintained under standard conditions at 37°C in a 5% CO2 humidified incubator and passaged before reaching confluency. Cell cultures were routinely tested for mycoplasma contamination by PCR. Cells were passaged with or without IFNγ for at least 2 weeks, samples are then harvested for RNA-seq. Human cells (A375) were treated with 20 IU/ml IFNγ, mouse cells with 50 IU/ml IFNγ, replacing medium and IFNγ every 72 h with passaging of cells as necessary for 2 weeks. Recombinant human IFNγ (PHC4031) and recombinant mouse IFNγ (PMC4031) were purchased from Gibco. Growth conditions - This submission studied the effect of IFNγ stimulation on 6 cancer cell lines. A375 (https://www.cellosaurus.org/CVCL_0132) and 501-mel (https://www.cellosaurus.org/CVCL_4633) were human melanoma cell lines. B16-F10 (https://www.cellosaurus.org/CVCL_0159), 5555 (provided by Santiago Zelenay, The University of Manchester) and YUMM2.1 (https://www.cellosaurus.org/CVCL_JK30) mouse melanoma cell lines. MC38 (https://www.cellosaurus.org/CVCL_B288) mouse colon cancer cell line. Human cell lines were maintained in DMEM (high glucose) medium. Mouse cell lines were maintained in RPMI-1640 (Sigma-Aldrich) medium. All growth media were supplemented with 10% fetal bovine serum (FBS; Life Technologies), 1% penicillin-streptomycin (P/S; Sigma-Aldrich). All cells were maintained under standard conditions at 37°C in a 5% CO2 humidified incubator and passaged before reaching confluency. Cell cultures were routinely tested for mycoplasma contamination by PCR. Cells were passaged with or without IFNγ for at least 2 weeks, samples are then harvested for RNA-seq. Human cells (A375) were treated with 20 IU/ml IFNγ, mouse cells with 50 IU/ml IFNγ, replacing medium and IFNγ every 72 h with passaging of cells as necessary for 2 weeks. Recombinant human IFNγ (PHC4031) and recombinant mouse IFNγ (PMC4031) were purchased from Gibco. Total RNA was isolated using the RNAeasy mini kit. RNA integrity was assessed on the Agilent 2200 TapeStation (Agilent Technologies). TruSeq Stranded mRNA assay (Illumina)

text: melanoma

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 13


E-MTAB-12196:Sample 14

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC first public

text: public

text: E-MTAB-12196:Sample 14

text: ArrayExpress

text: house mouse

text: melanoma

text: not applicable

text: Mus musculus

text: Mus musculus

text: skin

characteristic: organism part

text: Mus musculus

text: Sample 14


E-MTAB-12196:Sample 15

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12196:Sample 15

text: ArrayExpress

text: house mouse

text: melanoma

text: not applicable

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 15


E-MTAB-12196:Sample 16

text: UNIVERSITY OF MANCHESTER

text: public

characteristic: SRA accession

text: E-MTAB-12196:Sample 16

text: ArrayExpress

text: house mouse

text: melanoma

text: not applicable

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 16

characteristic: title


E-MTAB-12196:Sample 17

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12196:Sample 17

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: melanoma

text: not applicable

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 17


E-MTAB-12196:Sample 18

characteristic: ENA-CHECKLIST

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12196:Sample 18

text: ArrayExpress

text: house mouse

characteristic: description

text: melanoma

text: not applicable

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 18


E-MTAB-12196:Sample 1

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12196:Sample 1

text: ArrayExpress

text: human

text: melanoma

characteristic: disease

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin

text: Homo sapiens

text: Sample 1


E-MTAB-12196:Sample 2

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

text: public

text: E-MTAB-12196:Sample 2

text: ArrayExpress

text: human

text: melanoma

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: skin

text: Homo sapiens

text: Sample 2


E-MTAB-12196:Sample 3

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12196:Sample 3

characteristic: Submitter Id

text: ArrayExpress

text: human

text: melanoma

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin

text: Homo sapiens

text: Sample 3


E-MTAB-12196:Sample 4

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12196:Sample 4

text: ArrayExpress

text: human

text: melanoma

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: skin

text: Homo sapiens

text: Sample 4


E-MTAB-12196:Sample 5

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC first public

text: public

text: E-MTAB-12196:Sample 5

text: ArrayExpress

text: human

text: melanoma

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin

characteristic: organism part

text: Homo sapiens

text: Sample 5


E-MTAB-12196:Sample 6

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12196:Sample 6

text: ArrayExpress

text: human

text: melanoma

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin

text: Homo sapiens

text: Sample 6


E-MTAB-12196:Sample 7

text: UNIVERSITY OF MANCHESTER

text: public

characteristic: SRA accession

text: E-MTAB-12196:Sample 7

text: ArrayExpress

text: human

text: melanoma

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin

text: Homo sapiens

text: Sample 7

characteristic: title


E-MTAB-12196:Sample 8

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12196:Sample 8

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

text: melanoma

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin

text: Homo sapiens

text: Sample 8


E-MTAB-12196:Sample 9

characteristic: ENA-CHECKLIST

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12196:Sample 9

text: ArrayExpress

text: human

characteristic: description

text: melanoma

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin

text: Homo sapiens

text: Sample 9


E-MTAB-12196:Sample 10

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12196:Sample 10

text: ArrayExpress

text: human

text: melanoma

characteristic: disease

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin

text: Homo sapiens

text: Sample 10


E-MTAB-12196:Sample 11

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

text: public

text: E-MTAB-12196:Sample 11

text: ArrayExpress

text: human

text: melanoma

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: skin

text: Homo sapiens

text: Sample 11


E-MTAB-12196:Sample 12

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12196:Sample 12

characteristic: Submitter Id

text: ArrayExpress

text: human

text: melanoma

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin

text: Homo sapiens

text: Sample 12


E-MTAB-12196:Sample 19

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12196:Sample 19

text: ArrayExpress

text: house mouse

text: melanoma

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 19


E-MTAB-12196:Sample 20

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC first public

text: public

text: E-MTAB-12196:Sample 20

text: ArrayExpress

text: house mouse

text: melanoma

text: not applicable

text: Mus musculus

text: Mus musculus

text: skin

characteristic: organism part

text: Mus musculus

text: Sample 20


E-MTAB-12196:Sample 21

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12196:Sample 21

text: ArrayExpress

text: house mouse

text: melanoma

text: not applicable

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 21


E-MTAB-12196:Sample 22

text: UNIVERSITY OF MANCHESTER

text: public

characteristic: SRA accession

text: E-MTAB-12196:Sample 22

text: ArrayExpress

text: house mouse

text: melanoma

text: not applicable

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 22

characteristic: title


E-MTAB-12196:Sample 23

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12196:Sample 23

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: melanoma

text: not applicable

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 23


E-MTAB-12196:Sample 24

characteristic: ENA-CHECKLIST

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12196:Sample 24

text: ArrayExpress

text: house mouse

characteristic: description

text: melanoma

text: not applicable

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 24


E-MTAB-12196:Sample 25

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12196:Sample 25

text: ArrayExpress

text: house mouse

text: colon adenocarcinoma

characteristic: disease

text: not applicable

text: Mus musculus

text: Mus musculus

text: colon

text: Mus musculus

text: Sample 25


E-MTAB-12196:Sample 26

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

text: public

text: E-MTAB-12196:Sample 26

text: ArrayExpress

text: house mouse

text: colon adenocarcinoma

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: colon

text: Mus musculus

text: Sample 26


E-MTAB-12196:Sample 27

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12196:Sample 27

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: colon adenocarcinoma

text: not applicable

text: Mus musculus

text: Mus musculus

text: colon

text: Mus musculus

text: Sample 27


E-MTAB-12196:Sample 28

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12196:Sample 28

text: ArrayExpress

text: house mouse

text: colon adenocarcinoma

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: colon

text: Mus musculus

text: Sample 28


E-MTAB-12196:Sample 29

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC first public

text: public

text: E-MTAB-12196:Sample 29

text: ArrayExpress

text: house mouse

text: colon adenocarcinoma

text: not applicable

text: Mus musculus

text: Mus musculus

text: colon

characteristic: organism part

text: Mus musculus

text: Sample 29


E-MTAB-12196:Sample 30

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12196:Sample 30

text: ArrayExpress

text: house mouse

text: colon adenocarcinoma

text: not applicable

text: Mus musculus

text: Mus musculus

text: colon

text: Mus musculus

text: Sample 30


E-MTAB-12196:Sample 31

text: UNIVERSITY OF MANCHESTER

text: public

characteristic: SRA accession

text: E-MTAB-12196:Sample 31

text: ArrayExpress

text: house mouse

text: melanoma

text: not applicable

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 31

characteristic: title


E-MTAB-12196:Sample 32

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12196:Sample 32

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: melanoma

text: not applicable

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 32


E-MTAB-12196:Sample 33

characteristic: ENA-CHECKLIST

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12196:Sample 33

text: ArrayExpress

text: house mouse

characteristic: description

text: melanoma

text: not applicable

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 33


E-MTAB-12196:Sample 34

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12196:Sample 34

text: ArrayExpress

text: house mouse

text: melanoma

characteristic: disease

text: not applicable

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 34


E-MTAB-12196:Sample 35

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

text: public

text: E-MTAB-12196:Sample 35

text: ArrayExpress

text: house mouse

text: melanoma

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 35


E-MTAB-12196:Sample 36

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12196:Sample 36

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: melanoma

text: not applicable

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 36


text: Plasmodium falciparum

characteristic: ArrayExpress-SPECIES

characteristic: External Id

text: SC

text: public

text: Plasmodium falciparum

text: Plasmodium falciparum RO-33

characteristic: organism

text: Plasmodium falciparum RO-33

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Plasmodium falciparum

text: Dexiostoma campyla

text: Dexiostoma campyla

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Plasmodium falciparum

text: Colpidium colpoda

text: Colpidium colpoda

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Moneuplotes crassus

text: Moneuplotes crassus

text: Plasmodium falciparum

characteristic: title


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

characteristic: common name

text: Euplotes eurystomus

text: Euplotes eurystomus

text: Plasmodium falciparum


text: Plasmodium falciparum

characteristic: ENA-CHECKLIST

text: SC

text: public

text: Plasmodium falciparum

text: Euplotes woodruffi

text: Euplotes woodruffi

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Oxytricha fallax

text: Oxytricha fallax

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Stylonychia lemnae

text: Stylonychia lemnae

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Stylonychia mytilus

text: Stylonychia mytilus

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: Plasmodium falciparum

text: Blepharisma americanum

text: Blepharisma americanum

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum


text: Anopheles gambiae

text: SC

text: public

text: African malaria mosquito

text: Anopheles gambiae

text: Anopheles gambiae


text: Plasmodium falciparum

text: SC

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum


text: MPI for Marine Microbiology

text: public

tag: Namespace:MPI for Marine Microbiology

text: LMD-subsampled tissue after metabolic imaging

characteristic: description

text: Atlantic Ocean, Mid-Atlantic Ridge, Logatchev hydrothermal vent field

text: Bathymodiolus puteoserpentis

text: Bathymodiolus puteoserpentis

text: gill


characteristic: ENA first public

text: Sotio Biotech a.s.

text: public

tag: Namespace:Sotio Biotech a.s.

text: human

text: Homo sapiens

text: Homo sapiens


text: Sotio Biotech a.s.

text: public

text: house mouse

text: Mus musculus

text: Mus musculus

text: gDNA


Bcorolliflora

characteristic: ENA-FIRST-PUBLIC

text: public

text: Bcorolliflora

tag: Namespace:CENTER FOR BIOTECHNOLOGY, BIELEFELD UNIVERSITY

text: accession ID: BETA408

text: Beta corolliflora

text: Beta corolliflora

text: Wild beet plant sample for genome assembly and other analyses


Blomatogona

text: CENTER FOR BIOTECHNOLOGY, BIELEFELD UNIVERSITY

characteristic: INSDC center name

text: public

text: Blomatogona

text: accession ID: BETA674

text: Beta lomatogona

text: Beta lomatogona


Bmacrorhiza

text: public

text: Bmacrorhiza

text: accession ID: 18242

text: Beta macrorhiza

text: Beta macrorhiza


Pprocumbens

text: public

text: Pprocumbens

text: accession ID: BETA951

text: Patellifolia procumbens

text: Patellifolia procumbens


E-MTAB-12248:Sample 1

text: NTU

text: public

text: E-MTAB-12248:Sample 1

characteristic: age

text: ArrayExpress

characteristic: broker name

text: skeletal muscle myoblast

text: human

text: Protocols: Commerically available skeletal muscle cells from human donors (old myoblasts were purchased from Promocell (ref.no C-12530) and young myoblasts from Lonza (ref.no CC-2580)) were collected at three time-points across the experiment: at control (CTRL), end of proliferation (END PROL) and end of differentiation (END DIFF) during the repair period; and transferred to low binding/RNase free microcentrifuge tubes containing 350 µL RNeasy lysis Buffer (Qiagen) supplemented with ß-Mercaptoethanol (Sigma-Aldrich) to isolate total RNA using Rneasy Mini Kit (Qiagen). Myoblasts from aged (68 y/o) and young (20y/o) donors were proliferated in growth medium (DMEM+1%pen/strep+20%FBS) for 4 days before switching to differentiation medium (DMEM+1% pen/strep+2%HS+10ug/ml insulin for 6 days to induce myotube formation Differentiated myotubes were incubated with 12% BaCl2 for 2-4h to induce injury. Exposure to chemical injury was followed by the replication of the same experimental protocol used pre-injury. Following lysis, samples were homogenised and loaded onto Rneasy silica membrane for RNA binding. Concentrated and pure RNA was eluted in Rnase-Free water. Concentrations were quantified using spectrophotometry (NanoDrop™ 2000; Thermo Fisher Scientific). RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. To select cDNA fragments of preferentially 370~420 bp in length, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. At last, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system.

text: aged

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: DONOR 1

characteristic: individual

text: in vitro chemical injury

characteristic: injury

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: skeletal muscle tissue

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex

text: Sample 1


E-MTAB-12248:Sample 2

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NTU

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12248:Sample 2

characteristic: Submitter Id

text: ArrayExpress

text: skeletal muscle myoblast

text: human

text: aged post-proliferation

text: normal

text: wild type genotype

text: DONOR 1

text: in vitro chemical injury

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: skeletal muscle tissue

text: Homo sapiens

text: male

text: Sample 2

characteristic: title


E-MTAB-12248:Sample 3

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: NTU

characteristic: INSDC center name

text: public

text: E-MTAB-12248:Sample 3

characteristic: age

text: ArrayExpress

characteristic: broker name

text: skeletal muscle myoblast

text: human

characteristic: common name

characteristic: description

text: aged post-differentiation

characteristic: developmental stage

text: normal

text: wild type genotype

text: DONOR 1

text: in vitro chemical injury

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skeletal muscle tissue

text: Homo sapiens

text: male

text: Sample 3


E-MTAB-12248:Sample 4

characteristic: ENA-LAST-UPDATE

text: NTU

text: public

text: E-MTAB-12248:Sample 4

text: ArrayExpress

text: skeletal muscle myoblast

text: human

text: aged

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: DONOR 1

characteristic: individual

text: in vitro chemical injury

characteristic: injury

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: skeletal muscle tissue

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex

text: Sample 4


E-MTAB-12248:Sample 5

characteristic: External Id

text: NTU

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12248:Sample 5

characteristic: Submitter Id

text: ArrayExpress

text: skeletal muscle myoblast

text: human

text: aged post-proliferation

text: normal

text: wild type genotype

text: DONOR 1

text: in vitro chemical injury

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: skeletal muscle tissue

text: Homo sapiens

text: male

text: Sample 5

characteristic: title


E-MTAB-12248:Sample 6

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: NTU

characteristic: INSDC center name

text: public

text: E-MTAB-12248:Sample 6

characteristic: age

text: ArrayExpress

characteristic: broker name

text: skeletal muscle myoblast

text: human

characteristic: common name

characteristic: description

text: aged post-differentiation

characteristic: developmental stage

text: normal

text: wild type genotype

text: DONOR 1

text: in vitro chemical injury

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skeletal muscle tissue

text: Homo sapiens

text: male

text: Sample 6


E-MTAB-12248:Sample 7

characteristic: ENA-LAST-UPDATE

text: NTU

text: public

text: E-MTAB-12248:Sample 7

text: ArrayExpress

text: skeletal muscle myoblast

text: human

text: aged

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: DONOR 1

characteristic: individual

text: in vitro chemical injury

characteristic: injury

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: skeletal muscle tissue

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex

text: Sample 7


E-MTAB-12248:Sample 8

characteristic: External Id

text: NTU

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12248:Sample 8

characteristic: Submitter Id

text: ArrayExpress

text: skeletal muscle myoblast

text: human

text: aged post-proliferation

text: normal

text: wild type genotype

text: DONOR 1

text: in vitro chemical injury

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: skeletal muscle tissue

text: Homo sapiens

text: male

text: Sample 8

characteristic: title


E-MTAB-12248:Sample 9

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: NTU

characteristic: INSDC center name

text: public

text: E-MTAB-12248:Sample 9

characteristic: age

text: ArrayExpress

characteristic: broker name

text: skeletal muscle myoblast

text: human

characteristic: common name

characteristic: description

text: aged post-differentiation

characteristic: developmental stage

text: normal

text: wild type genotype

text: DONOR 1

text: in vitro chemical injury

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skeletal muscle tissue

text: Homo sapiens

text: male

text: Sample 9


E-MTAB-12248:Sample 10

characteristic: ENA-LAST-UPDATE

text: NTU

text: public

text: E-MTAB-12248:Sample 10

text: ArrayExpress

text: skeletal muscle myoblast

text: human

text: aged

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: DONOR 1

characteristic: individual

text: in vitro chemical injury

characteristic: injury

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: skeletal muscle tissue

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex

text: Sample 10


E-MTAB-12248:Sample 11

characteristic: External Id

text: NTU

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12248:Sample 11

characteristic: Submitter Id

text: ArrayExpress

text: skeletal muscle myoblast

text: human

text: aged post-proliferation

text: normal

text: wild type genotype

text: DONOR 1

text: in vitro chemical injury

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: skeletal muscle tissue

text: Homo sapiens

text: male

text: Sample 11

characteristic: title


E-MTAB-12248:Sample 12

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: NTU

characteristic: INSDC center name

text: public

text: E-MTAB-12248:Sample 12

characteristic: age

text: ArrayExpress

characteristic: broker name

text: skeletal muscle myoblast

text: human

characteristic: common name

characteristic: description

text: aged post-differentiation

characteristic: developmental stage

text: normal

text: wild type genotype

text: DONOR 1

text: in vitro chemical injury

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skeletal muscle tissue

text: Homo sapiens

text: male

text: Sample 12


E-MTAB-12248:Sample 13

characteristic: ENA-LAST-UPDATE

text: NTU

text: public

text: E-MTAB-12248:Sample 13

text: ArrayExpress

text: skeletal muscle myoblast

text: human

text: young

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: DONOR 2

characteristic: individual

text: in vitro chemical injury

characteristic: injury

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: skeletal muscle tissue

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex

text: Sample 13


E-MTAB-12248:Sample 14

characteristic: External Id

text: NTU

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12248:Sample 14

characteristic: Submitter Id

text: ArrayExpress

text: skeletal muscle myoblast

text: human

text: young post-proliferation

text: normal

text: wild type genotype

text: DONOR 2

text: in vitro chemical injury

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: skeletal muscle tissue

text: Homo sapiens

text: male

text: Sample 14

characteristic: title


E-MTAB-12248:Sample 15

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: NTU

characteristic: INSDC center name

text: public

text: E-MTAB-12248:Sample 15

characteristic: age

text: ArrayExpress

characteristic: broker name

text: skeletal muscle myoblast

text: human

characteristic: common name

characteristic: description

text: young post-differentiation

characteristic: developmental stage

text: normal

text: wild type genotype

text: DONOR 2

text: in vitro chemical injury

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skeletal muscle tissue

text: Homo sapiens

text: male

text: Sample 15


E-MTAB-12248:Sample 16

characteristic: ENA-LAST-UPDATE

text: NTU

text: public

text: E-MTAB-12248:Sample 16

text: ArrayExpress

text: skeletal muscle myoblast

text: human

text: young

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: DONOR 2

characteristic: individual

text: in vitro chemical injury

characteristic: injury

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: skeletal muscle tissue

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex

text: Sample 16