E-MTAB-12248:Sample 17

characteristic: External Id

text: NTU

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12248:Sample 17

characteristic: Submitter Id

text: ArrayExpress

text: skeletal muscle myoblast

text: human

text: young post-proliferation

text: normal

text: wild type genotype

text: DONOR 2

text: in vitro chemical injury

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: skeletal muscle tissue

text: Homo sapiens

text: male

text: Sample 17

characteristic: title


E-MTAB-12248:Sample 18

status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: NTU

characteristic: INSDC center name

text: public

text: E-MTAB-12248:Sample 18

unit: year

characteristic: age

text: ArrayExpress

characteristic: broker name

text: skeletal muscle myoblast

text: human

characteristic: common name

text: Protocols: Commerically available skeletal muscle cells from human donors (old myoblasts were purchased from Promocell (ref.no C-12530) and young myoblasts from Lonza (ref.no CC-2580)) were collected at three time-points across the experiment: at control (CTRL), end of proliferation (END PROL) and end of differentiation (END DIFF) during the repair period; and transferred to low binding/RNase free microcentrifuge tubes containing 350 µL RNeasy lysis Buffer (Qiagen) supplemented with ß-Mercaptoethanol (Sigma-Aldrich) to isolate total RNA using Rneasy Mini Kit (Qiagen). Myoblasts from aged (68 y/o) and young (20y/o) donors were proliferated in growth medium (DMEM+1%pen/strep+20%FBS) for 4 days before switching to differentiation medium (DMEM+1% pen/strep+2%HS+10ug/ml insulin for 6 days to induce myotube formation Differentiated myotubes were incubated with 12% BaCl2 for 2-4h to induce injury. Exposure to chemical injury was followed by the replication of the same experimental protocol used pre-injury. Following lysis, samples were homogenised and loaded onto Rneasy silica membrane for RNA binding. Concentrated and pure RNA was eluted in Rnase-Free water. Concentrations were quantified using spectrophotometry (NanoDrop™ 2000; Thermo Fisher Scientific). RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. To select cDNA fragments of preferentially 370~420 bp in length, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. At last, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system.

characteristic: description

text: young post-differentiation

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: DONOR 2

characteristic: individual

text: in vitro chemical injury

characteristic: injury

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: skeletal muscle tissue

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex

text: Sample 18


E-MTAB-12248:Sample 19

characteristic: External Id

text: NTU

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12248:Sample 19

characteristic: Submitter Id

text: ArrayExpress

text: skeletal muscle myoblast

text: human

text: young

text: normal

text: wild type genotype

text: DONOR 2

text: in vitro chemical injury

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: skeletal muscle tissue

text: Homo sapiens

text: male

text: Sample 19

characteristic: title


E-MTAB-12248:Sample 20

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: NTU

characteristic: INSDC center name

text: public

text: E-MTAB-12248:Sample 20

characteristic: age

text: ArrayExpress

characteristic: broker name

text: skeletal muscle myoblast

text: human

characteristic: common name

characteristic: description

text: young post-proliferation

text: normal

text: wild type genotype

text: DONOR 2

text: in vitro chemical injury

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skeletal muscle tissue

text: Homo sapiens

text: male

text: Sample 20


E-MTAB-12248:Sample 21

text: NTU

text: public

text: E-MTAB-12248:Sample 21

text: ArrayExpress

text: skeletal muscle myoblast

text: human

text: young post-differentiation

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: DONOR 2

characteristic: individual

text: in vitro chemical injury

characteristic: injury

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: skeletal muscle tissue

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex

text: Sample 21


E-MTAB-12248:Sample 22

characteristic: External Id

text: NTU

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12248:Sample 22

characteristic: Submitter Id

text: ArrayExpress

text: skeletal muscle myoblast

text: human

text: young

text: normal

text: wild type genotype

text: DONOR 2

text: in vitro chemical injury

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: skeletal muscle tissue

text: Homo sapiens

text: male

text: Sample 22

characteristic: title


E-MTAB-12248:Sample 23

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: NTU

characteristic: INSDC center name

text: public

text: E-MTAB-12248:Sample 23

characteristic: age

text: ArrayExpress

characteristic: broker name

text: skeletal muscle myoblast

text: human

characteristic: common name

characteristic: description

text: young post-differentiation

text: normal

text: wild type genotype

text: DONOR 2

text: in vitro chemical injury

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skeletal muscle tissue

text: Homo sapiens

text: male

text: Sample 23


text: Shanghai Tenth People's Hospital

text: public

tag: Namespace:Shanghai Tenth People's Hospital

text: ArrayExpress

text: Protocols: Strains were maintained at -80˚C. Strains were streaked on YPD plates. The plates were incubated at 37˚C overnight. Then several colonies were picked up and suspended in DDW. Cell density was adjusted to 10,000 cells/ml, then 100µl of cell suspension was plated on YPD plates. The plates were incubated at 37˚C overnight. Colonies were picked up and suspended in DDW, then cell pellets were obtained by centrifuging at highest speed for 1min. We used the phenol-chloroform method to extract Genomic DNA. Cell pellets were suspended in 200µl of lysis buffer, add 100µl of acid washed glass beads, and 0,2ml phenol:chloroform:isoamyl alcohol (25:24:1). Vortex for 30min at 4˚C. Add 200µl TE pH7.5. Centrifuge for 5min at maximum speed. Transfer aqueous layer to a new 1.5ml EP tube. Add 1ml 100% ethanol. Precipitate 1h at -20˚C. Pellet DNA by centrifuging for 10min at maximum speed. Discard the supernatant. Add 400µl TE pH 7.5. Add 3ul of RNase A (10mg/ml). Incubate at 37˚C for 30min. Add 10µl of 3M NaOAc and 1ml of 100% ethanol. Mix by inversion. Precipitate 30min at -20˚C. Pellet DNA by centrifuging for 10min at maximum speed. Discard supernatant. Air-dry the pellet overnight. Resuspend in 50µl water. "1µg Genomic DNA was randomly fragmented by Covaris, followed by fragments selection by Agencourt AMPure XP-Medium kit to an average size of 200~400 bp. Selected fragments were end repaired and 3\""adenylate, then the adaptors were ligated to the end of these 3\""adenylate fragments. The products were amplified by PCR and purified by the Agencourt AMPure XP-Medium kit. The purified double stranded PCR products were heat denatured to single strand, then circularised by the splint oligo sequence. The single strand circle DNA (ssCirDNA) were formatted as the final library and qualified by QC."

text: euploid

characteristic: genotype

text: not applicable

characteristic: isolate

text: Cryptococcus neoformans var. grubii H99

text: Cryptococcus neoformans var. grubii H99

text: whole organism

characteristic: organism part

text: Cryptococcus neoformans var. grubii H99


characteristic: External Id

text: Shanghai Tenth People's Hospital

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: aneuploid

text: not applicable

text: Cryptococcus neoformans var. grubii H99

text: Cryptococcus neoformans var. grubii H99

text: whole organism

text: Cryptococcus neoformans var. grubii H99


text: CSISP

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-13-10-2022-12:50:02:324-44098

characteristic: Submitter Id

text: anthropogenic terrestrial biome

characteristic: broad-scale environmental context

characteristic: collection date

text: human

text: BAL

text: bodily fluid material

characteristic: environmental medium

text: France

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: urban biome

characteristic: local environmental context

text: Homo sapiens

characteristic: project name

text: Homo sapiens

text: Illumina Miseq

characteristic: sequencing method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: CSISP

characteristic: INSDC center name

text: public

text: ena-SAMPLE-TAB-13-10-2022-12:50:02:325-44099

text: anthropogenic terrestrial biome

text: human

characteristic: common name

text: BAL

characteristic: description

text: bodily fluid material

text: France

text: urban biome

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Illumina Miseq

characteristic: title


characteristic: External Id

text: CSISP

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-13-10-2022-12:50:02:325-44100

characteristic: Submitter Id

text: anthropogenic terrestrial biome

characteristic: broad-scale environmental context

text: human

text: BAL

text: bodily fluid material

text: France

text: urban biome

text: Homo sapiens

text: Homo sapiens

text: Illumina Miseq


text: CSISP

text: public

text: ena-SAMPLE-TAB-13-10-2022-12:50:02:325-44101

text: anthropogenic terrestrial biome

characteristic: collection date

text: human

text: BAL

text: bodily fluid material

characteristic: environmental medium

text: France

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: urban biome

characteristic: local environmental context

text: Homo sapiens

characteristic: project name

text: Homo sapiens

text: Illumina Miseq


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: CSISP

characteristic: INSDC center name

text: public

text: ena-SAMPLE-TAB-13-10-2022-12:50:02:325-44102

text: anthropogenic terrestrial biome

text: human

characteristic: common name

text: BAL

characteristic: description

text: bodily fluid material

text: France

text: urban biome

text: Homo sapiens

text: Homo sapiens

text: Illumina Miseq

characteristic: sequencing method


characteristic: External Id

text: CSISP

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-13-10-2022-12:50:02:325-44103

text: anthropogenic terrestrial biome

text: human

text: BAL

text: bodily fluid material

text: France

text: urban biome

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Illumina Miseq

characteristic: title


text: CSISP

text: public

text: ena-SAMPLE-TAB-13-10-2022-12:50:02:325-44104

characteristic: Submitter Id

text: anthropogenic terrestrial biome

characteristic: broad-scale environmental context

characteristic: collection date

text: human

text: BAL

text: bodily fluid material

characteristic: environmental medium

text: France

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: urban biome

text: Homo sapiens

text: Homo sapiens

text: Illumina Miseq


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: CSISP

characteristic: INSDC center name

text: public

text: ena-SAMPLE-TAB-13-10-2022-12:50:02:325-44105

text: anthropogenic terrestrial biome

text: human

characteristic: common name

text: BAL

text: bodily fluid material

text: France

text: urban biome

characteristic: local environmental context

text: Homo sapiens

characteristic: project name

text: Homo sapiens

text: Illumina Miseq


characteristic: External Id

text: CSISP

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-13-10-2022-12:50:02:325-44106

text: anthropogenic terrestrial biome

text: human

text: BAL

characteristic: description

text: bodily fluid material

text: France

text: urban biome

text: Homo sapiens

text: Homo sapiens

text: Illumina Miseq

characteristic: sequencing method


text: CSISP

text: public

text: ena-SAMPLE-TAB-13-10-2022-12:50:02:325-44107

characteristic: Submitter Id

text: anthropogenic terrestrial biome

characteristic: broad-scale environmental context

characteristic: collection date

text: human

text: BAL

text: bodily fluid material

characteristic: environmental medium

text: France

characteristic: geographic location (country and/or sea)

text: urban biome

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Illumina Miseq

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: CSISP

characteristic: INSDC center name

text: public

text: ena-SAMPLE-TAB-13-10-2022-12:50:02:325-44108

text: anthropogenic terrestrial biome

text: human

characteristic: common name

text: BAL

text: bodily fluid material

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: urban biome

text: Homo sapiens

text: Homo sapiens

text: Illumina Miseq


characteristic: External Id

text: CSISP

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-13-10-2022-12:50:02:325-44109

text: anthropogenic terrestrial biome

text: human

text: BAL

text: bodily fluid material

text: France

text: urban biome

characteristic: local environmental context

text: Homo sapiens

characteristic: project name

text: Homo sapiens

text: Illumina Miseq


text: CSISP

text: public

text: ena-SAMPLE-TAB-13-10-2022-12:50:02:325-44110

characteristic: Submitter Id

text: anthropogenic terrestrial biome

characteristic: broad-scale environmental context

characteristic: collection date

text: human

text: BAL

characteristic: description

text: bodily fluid material

characteristic: environmental medium

text: France

characteristic: geographic location (country and/or sea)

text: urban biome

text: Homo sapiens

text: Homo sapiens

text: Illumina Miseq

characteristic: sequencing method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: CSISP

characteristic: INSDC center name

text: public

text: ena-SAMPLE-TAB-13-10-2022-12:50:02:326-44111

text: anthropogenic terrestrial biome

text: human

characteristic: common name

text: BAL

text: bodily fluid material

text: France

text: urban biome

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Illumina Miseq

characteristic: title


characteristic: External Id

text: CSISP

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-13-10-2022-12:50:02:326-44112

text: anthropogenic terrestrial biome

text: human

text: BAL

text: bodily fluid material

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: urban biome

text: Homo sapiens

text: Homo sapiens

text: Illumina Miseq


text: CSISP

text: public

text: ena-SAMPLE-TAB-13-10-2022-12:50:02:326-44113

characteristic: Submitter Id

text: anthropogenic terrestrial biome

characteristic: broad-scale environmental context

characteristic: collection date

text: human

text: BAL

characteristic: description

text: bodily fluid material

characteristic: environmental medium

text: France

text: urban biome

characteristic: local environmental context

text: Homo sapiens

characteristic: project name

text: Homo sapiens

text: Illumina Miseq


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: CSISP

characteristic: INSDC center name

text: public

text: ena-SAMPLE-TAB-13-10-2022-12:50:02:326-44114

text: anthropogenic terrestrial biome

text: human

characteristic: common name

text: BAL

text: bodily fluid material

text: France

characteristic: geographic location (country and/or sea)

text: urban biome

text: Homo sapiens

text: Homo sapiens

text: Illumina Miseq

characteristic: sequencing method


characteristic: External Id

text: CSISP

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-13-10-2022-12:50:02:326-44115

text: anthropogenic terrestrial biome

text: human

text: BAL

text: bodily fluid material

text: France

text: urban biome

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Illumina Miseq

characteristic: title


text: CSISP

text: public

text: ena-SAMPLE-TAB-13-10-2022-12:50:02:326-44116

characteristic: Submitter Id

text: anthropogenic terrestrial biome

characteristic: broad-scale environmental context

characteristic: collection date

text: human

text: BAL

characteristic: description

text: bodily fluid material

characteristic: environmental medium

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: urban biome

text: Homo sapiens

text: Homo sapiens

text: Illumina Miseq


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: CSISP

characteristic: INSDC center name

text: public

text: ena-SAMPLE-TAB-13-10-2022-12:50:02:326-44117

text: anthropogenic terrestrial biome

text: human

characteristic: common name

text: BAL

text: bodily fluid material

text: France

text: urban biome

characteristic: local environmental context

text: Homo sapiens

characteristic: project name

text: Homo sapiens

text: Illumina Miseq


characteristic: External Id

text: CSISP

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-13-10-2022-12:50:02:326-44118

text: anthropogenic terrestrial biome

text: human

text: BAL

text: bodily fluid material

text: France

characteristic: geographic location (country and/or sea)

text: urban biome

text: Homo sapiens

text: Homo sapiens

text: Illumina Miseq

characteristic: sequencing method


text: CSISP

text: public

text: ena-SAMPLE-TAB-13-10-2022-12:50:02:326-44119

characteristic: Submitter Id

text: anthropogenic terrestrial biome

characteristic: broad-scale environmental context

characteristic: collection date

text: human

text: BAL

characteristic: description

text: bodily fluid material

characteristic: environmental medium

text: France

text: urban biome

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Illumina Miseq

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: CSISP

characteristic: INSDC center name

text: public

text: ena-SAMPLE-TAB-13-10-2022-12:50:02:326-44120

text: anthropogenic terrestrial biome

text: human

characteristic: common name

text: BAL

text: bodily fluid material

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: urban biome

text: Homo sapiens

text: Homo sapiens

text: Illumina Miseq


characteristic: External Id

text: CSISP

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-13-10-2022-12:50:02:327-44121

text: anthropogenic terrestrial biome

text: human

text: BAL

text: bodily fluid material

text: France

text: urban biome

characteristic: local environmental context

text: Homo sapiens

characteristic: project name

text: Homo sapiens

text: Illumina Miseq


text: CSISP

text: public

text: ena-SAMPLE-TAB-13-10-2022-12:50:02:327-44122

characteristic: Submitter Id

text: anthropogenic terrestrial biome

characteristic: broad-scale environmental context

characteristic: collection date

text: human

text: BAL

characteristic: description

text: bodily fluid material

characteristic: environmental medium

text: France

characteristic: geographic location (country and/or sea)

text: urban biome

text: Homo sapiens

text: Homo sapiens

text: Illumina Miseq

characteristic: sequencing method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: CSISP

characteristic: INSDC center name

text: public

text: ena-SAMPLE-TAB-13-10-2022-12:50:02:327-44123

text: anthropogenic terrestrial biome

text: human

characteristic: common name

text: BAL

text: bodily fluid material

text: France

text: urban biome

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Illumina Miseq

characteristic: title


characteristic: External Id

text: CSISP

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-13-10-2022-12:50:02:327-44124

text: anthropogenic terrestrial biome

text: human

text: BAL

text: bodily fluid material

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: urban biome

text: Homo sapiens

text: Homo sapiens

text: Illumina Miseq


text: CSISP

text: public

text: ena-SAMPLE-TAB-13-10-2022-12:50:02:327-44125

characteristic: Submitter Id

text: anthropogenic terrestrial biome

characteristic: broad-scale environmental context

characteristic: collection date

text: human

text: BAL

characteristic: description

text: bodily fluid material

characteristic: environmental medium

text: France

text: urban biome

characteristic: local environmental context

text: Homo sapiens

characteristic: project name

text: Homo sapiens

text: Illumina Miseq


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: CSISP

characteristic: INSDC center name

text: public

text: ena-SAMPLE-TAB-13-10-2022-12:50:02:327-44126

text: anthropogenic terrestrial biome

text: human

characteristic: common name

text: BAL

text: bodily fluid material

text: France

characteristic: geographic location (country and/or sea)

text: urban biome

text: Homo sapiens

text: Homo sapiens

text: Illumina Miseq

characteristic: sequencing method


characteristic: External Id

text: CSISP

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-13-10-2022-12:50:02:327-44127

text: anthropogenic terrestrial biome

text: human

text: BAL

text: bodily fluid material

text: France

text: urban biome

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Illumina Miseq

characteristic: title


text: CSISP

text: public

text: ena-SAMPLE-TAB-13-10-2022-12:50:02:327-44128

characteristic: Submitter Id

text: anthropogenic terrestrial biome

characteristic: broad-scale environmental context

characteristic: collection date

text: human

text: BAL

characteristic: description

text: bodily fluid material

characteristic: environmental medium

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: urban biome

text: Homo sapiens

text: Homo sapiens

text: Illumina Miseq


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: CSISP

characteristic: INSDC center name

text: public

text: ena-SAMPLE-TAB-13-10-2022-12:50:02:327-44129

text: anthropogenic terrestrial biome

text: human

characteristic: common name

text: BAL

text: bodily fluid material

text: France

text: urban biome

characteristic: local environmental context

text: Homo sapiens

characteristic: project name

text: Homo sapiens

text: Illumina Miseq


characteristic: External Id

text: CSISP

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-13-10-2022-12:50:02:327-44130

text: anthropogenic terrestrial biome

text: human

text: BAL

text: bodily fluid material

text: France

characteristic: geographic location (country and/or sea)

text: urban biome

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text: Protocols: Samples were obtained by dissecting the melanoma tumour from the animal skin, removing the complete skin from the tumour. Scissors and scalpel were used to remove the tissue and then it was placed in cold PBS on ice until tissue processing. A piece of 100 mg was weighed and chopped using sharp razors and scalpels in cold DMEM without FBS and P/S on ice. A final concentration of 0.25 mg/mL of Liberase DH (Merck, 54010544001), 0.55 mg/mL of Dispase II (Merck, 4942078001) and 150 U/mL of DNAse (Roche, 11284932001) was added in a total of 1 mL of solution. Samples were incubated at 37°C for 30 min and then homogenised with increasing gauge syringes up to 25G. Then, samples were centrifuged at 300g 4°C 5 min and 1X red-blood lysis buffer (Biolegend, 420301) was applied for 5 min RT and then filtered through a 40uM cell strainer. Samples were centrifuged again for at 300g 5 min 4°C , resuspended in FACS buffer (sterile-filtered 1% FCS, 2mM EDTA, 25mM HEPES in 1X PBS) and 1:50 TruStain FcX mouse antibody (Biolegend, 156604) for 10 min on ice and darkness. Following, incubation with anti-CD45-PE antibody (Biolegend, 103106) 1:100 and 1ug/mL DAPI for 30 min on ice and darkness. Finally, samples were washed with FACS buffer, centrifuged at 300g 4°C 5 min twice. FACS isolation was issued gating DAPI negative population (live cells) and double discrimination of singles and doublets. Then CD45-PE+ cells were sorted into individual tubes per group condition, 15000 cells per group. Cell integrity and number was assessed by trypan blue and bright-field microscopy in every condition. Live CD45-PE+ cells were encapsulated intro droplets and libraries were prepared using Chromium Single Cell Reagents kit v3.1 according to the manufacturer’s protocol Animals were culled humanely when they reached the maximum volume or when the experimental endpoint dictated. Tumours were removed from the animal skin, removing the complete skin from the tumour. Scissors and scalpel were used to remove the tissue and then it was placed in cold PBS on ice until tissue processing. Upon tumour resection, the samples were divided (whenever possible given the tumour size) for RNA extraction, whole tissue snap freeze and OCT tissue embedding and/or the different procedures described in the article. Mice were given subcutaneous injections of a BRAFV600mut melanoma cell line (5555) of 5 x 106 cells on the right flank of immunocompetent mice on day 0. Animals were monitored daily when tumour was evident and palpable (70 mm3) Around day 6 post injection (6DPI), animals were then divided into groups and treated accordingly. Ranolazine group was given 150 mg/kg intraperitoneal injection of Ranolazine (MedChemExpress, HY-17401) on day 6DPI and 300 mg/kg from 7DPI to end point. PD-L1 group was given 10mg/kg anti-PD-L1 (BioxCell, PD-L1 B7-H1, BE0101) starting on 6DPI and every 3 days for a total of 5 doses until end point. PD-L1 + Ranolazine group was given both treatments as described previously. Control group was injected with PBS daily until endpoint. Mice were sacrificed when tumours volume reached below 1500 mm3 and culled humanely. RNA extraction is performed within single cell v3.1 gel beads as described by manufacturer 10X genomics. Reverse transcription is then procured within each sample and the results is GEMs with barcoded full-length cDNA from poly-adenylated mRNA.Immediately, following the Gel GEM generation, the Gel Bead is dissolved, primers are released, and any copartitioned cell is lysed. Primer containing: [i] an Illumina TruSeq Read 1 (read 1 sequencing primer) [ii] 16 nt 10x Barcode [iii] 12 nt unique molecular identifier (UMI) [iv] 30 nt poly(dT) sequence are mixed with the cell lysate and a Master Mix containing reverse transcription (RT) reagents. Incubation of the GEMs produces barcoded, full-length cDNA from poly-adenylated mRNA. For the generation of the libraries, the kit foe Next GEM Single Cell 3’ Reagents Kits v3.1 (Dual Index) has been used. After incubation, GEMs are broken, and pooled fractions are recovered. Silane magnetic beads are used to purify the first-strand cDNA from the post GEM-RT reaction mixture, which includes biochemical leftover reagents and primers. Barcoded, full-length cDNA is amplified via PCR to generate sufficient mass for library construction.

text: adult

characteristic: developmental stage

text: melanoma

characteristic: disease

text: wild type genotype

characteristic: genotype

text: injected with 5555 BRAFv600 mutant mouse melanoma cells

characteristic: growth condition

text: Control 1

characteristic: individual

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: neoplasm

characteristic: organism part

text: Replicate 1

characteristic: replicate

text: Mus musculus

text: male

characteristic: sex

characteristic: strain

text: Control


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IRB Barcelona / BIST

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: house mouse

characteristic: description

text: adult

text: melanoma

text: wild type genotype

text: PDL1 1

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: neoplasm

text: Replicate 1

text: Mus musculus

text: male

characteristic: title


text: IRB Barcelona / BIST

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell count

text: house mouse

characteristic: common name

text: adult

characteristic: developmental stage

text: melanoma

characteristic: disease

text: wild type genotype

characteristic: genotype

characteristic: growth condition

text: PDL1_Rano 1

text: not applicable

text: Mus musculus

text: Mus musculus

text: neoplasm

text: Replicate 1

text: Mus musculus

text: male


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IRB Barcelona / BIST

text: public

text: ArrayExpress

text: house mouse

text: adult

text: melanoma

text: wild type genotype

text: Rano 1

characteristic: individual

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: neoplasm

characteristic: organism part

text: Replicate 1

characteristic: replicate

text: Mus musculus

text: male

characteristic: sex

characteristic: strain

text: Rano


text: Sanger Institute

characteristic: GAL

text: NOT PROVIDED

text: Tree of Life Programme

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Tree of Life Programme

characteristic: Submitter Id

text: NOT COLLECTED

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: geographic location (latitude)

text: not collected

characteristic: geographic location (longitude)

text: England

characteristic: geographic location (region and locality)

text: NOT COLLECTED

characteristic: habitat

text: NOT COLLECTED

text: NOT COLLECTED

text: adult

characteristic: lifestage

text: Biomphalaria glabrata

characteristic: organism

text: WHOLE ORGANISM

text: ToL

characteristic: project name

text: Biomphalaria glabrata

text: NOT COLLECTED

text: N

characteristic: symbiont

characteristic: title

characteristic: tolid


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Shanghai Tenth People's Hospital

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

characteristic: broker name

text: Protocols: Cells were grown in YPD broth at 37˚C from OD600=0.01 till OD600=1.0. Pellets of 5ml cell culture were obtained by centrifuging at maximum speed for 1min. the pellets were fast frozen in liquid nitrogen. RNA was extracted using the TRIzol method following the manufacturer's protocol. Cell pellets were transferred from liquid nitrogen into the 2ml tub containing 1.5ml Trizol reagent. The mix was shared for 3min, and kept for 5in at room temperature, then centrifuged at 10,000xg for 5min at 4˚C. The supernatant was added 200µl of chloroform/isoamyl alcohol (24:1) with 1ml lysis reagents. After centrifuged at 10,000g for 10min at 4˚C, the supernatant was transferred into another new tube with equal volume of isopropanol and put in the refrigerator at -20˚C for 1h. After centrifuged at 13600xg for 20min at 4˚C, the supernatant was precipitated by 1ml of 75% ethanol and let dry for 3-5min. The RNA pellet was dissolved with 30-100µl of DEPC water. The concentration of the extracted RNA was determined using a Nanodrop system (NanoDrop, Madison, USA), and the integrity of the RNA was examined by the RNA integrity number (RIN) using an Agilent 2100 bioanalyzer (Agilent, Santa Clara, USA) buffer at appropriate temperature. Then First-strand cDNA was generated using random hexamer-primed reverse transcription, followed by a second-strand cDNA synthesis. afterwards, A-Tailing Mix and RNA Index Adapters were added by incubating to end repair. The cDNA fragments obtained from previous step were amplified by PCR, and products were purified by Ampure XP Beads, then dissolved in EB solution. The product was validated on the Agilent Technologies 2100 bioanalyzer for quality control. The double stranded PCR products from previous step were heated denatured and circularized by the splint oligo sequence to get the final library. The single strand circle DNA (ssCir DNA) was formatted as the final library.

characteristic: description

text: log phase

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: YPD medium

characteristic: growth condition

text: not applicable

characteristic: isolate

text: Candida parapsilosis

text: Candida parapsilosis

text: whole organism

characteristic: organism part

text: Candida parapsilosis

characteristic: strain


text: Shanghai Tenth People's Hospital

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: log phase

text: trisomy of chromosome 5

text: YPD medium

text: not applicable

text: Candida parapsilosis

characteristic: organism

text: Candida parapsilosis

text: whole organism

text: Candida parapsilosis

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Shanghai Tenth People's Hospital

text: public

text: ArrayExpress

text: log phase

text: trisomy of chromosome 1

text: YPD medium

text: not applicable

text: Candida parapsilosis

text: Candida parapsilosis

text: whole organism

text: Candida parapsilosis


text: OLivier

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Conn, B.J. 5272, (K)

text: Indian almond

characteristic: common name

text: Terminalia catappa

text: Terminalia catappa

text: Terminalia catappa


text: OLivier

text: public

text: Lau, C.P. 27, (K)

characteristic: description

text: Ehrharta brevifolia

text: Ehrharta brevifolia

text: Combretum alfredii


text: OLivier

text: public

text: van dr Burgt, X. 1653, (K); K001061580

text: Strephonema pseudocola

text: Strephonema pseudocola

text: Strephonema pseudocola


text: OLivier

text: public

text: Maurin, O. 16, (JRAU)

text: Combretum paniculatum

text: Combretum paniculatum

text: Combretum paniculatum


text: OLivier

text: public

text: Maurin, O. 1041, (BNRH)

text: Combretum zeyheri

text: Combretum zeyheri

text: Combretum zeyheri


text: OLivier

text: public

text: Maurin, O. 478, (SKU)

text: Terminalia sericea

text: Terminalia sericea

text: Terminalia sericea


text: OLivier

text: public

text: Maurin, O. 1524, (BNRH)

text: Combretum collinum

text: Combretum collinum

text: Combretum collinum


text: OLivier

text: public

text: Maurin, O. 238, (SKU)

text: Combretum hereroense

text: Combretum hereroense

text: Combretum hereroense


text: OLivier

text: public

text: Maurin, O. 2350, (BNRH)

text: Combretum adenogonium

text: Combretum adenogonium

text: Combretum adenogonium


text: OLivier

text: public

text: Maurin, O. 22, (JRAU)

text: Combretum bracteosum

text: Combretum bracteosum

text: Combretum bracteosum


text: OLivier

text: public

text: Maurin, O. 11, (JRAU)

text: Combretum caffrum

text: Combretum caffrum

text: Combretum caffrum


text: OLivier

text: public

text: Maurin, O. 4085, (BNRH)

text: Combretum celastroides

text: Combretum celastroides

text: Combretum celastroides


text: OLivier

text: public

text: Maurin, O. 1025, (BNRH)

text: Terminalia stenostachya

text: Terminalia stenostachya

text: Combretum englerii


text: OLivier

text: public

text: Maurin, O. 201, (SKU)

text: Combretum erythrophyllum

text: Combretum erythrophyllum

text: Combretum erythrophyllum


text: OLivier

text: public

text: Maurin, O. 3521, (BNRH)

text: Combretum kirkii

text: Combretum kirkii

text: Combretum kirkii


text: OLivier

text: public

text: Maurin, O. 36, (JRAU)

text: Combretum kraussii

text: Combretum kraussii

text: Combretum kraussii


text: OLivier

text: public

text: Maurin, O. 205, (SKU)

text: Combretum microphyllum

text: Combretum microphyllum

text: Combretum microphyllum


text: OLivier

text: public

text: Bryden, R;154, (SKU)

text: Combretum mkuzense

text: Combretum mkuzense

text: Combretum mkuzense


text: OLivier

text: public

text: Maurin, O. 1011, (BNRH)

text: Combretum mossambicense

text: Combretum mossambicense

text: Combretum mossambicense


text: OLivier

text: public

text: van der Bank, M. 26, (JRAU)

text: Combretum nelsonii

text: Combretum nelsonii

text: Combretum nelsonii


text: OLivier

text: public

text: Maurin, O. 1052, (BNRH)

text: Combretum oxystachyum

text: Combretum oxystachyum

text: Combretum oxystachyum


text: OLivier

text: public

text: Maurin, O. 1285, (BNRH)

text: Combretum padoides

text: Combretum padoides

text: Combretum padoides


text: OLivier

text: public

text: Palgrave, M.C. 508, (CHER)

text: Combretum pisoniiflorum

text: Combretum pisoniiflorum

text: Combretum pisoniiflorum


text: OLivier

text: public

text: Potgieter, J.A. 46, (K)

text: Combretum psidioides

text: Combretum psidioides

text: Combretum psidioides


text: OLivier

text: public

text: Maurin, O. 1089, (BNRH)

text: Combretum tenuipes

text: Combretum tenuipes

text: Combretum tenuipes


text: OLivier

text: public

text: Maurin, O. 3406, (BNRH)

text: Combretum vendae

text: Combretum vendae

text: Combretum vendae


text: OLivier

text: public

text: Maurin, O. 1421, (BNRH)

text: Combretum woodii

text: Combretum woodii

text: Combretum woodii


text: OLivier

text: public

text: Maurin, O. 4147, (BNRH)

text: Terminalia mollis

text: Terminalia mollis

text: Terminalia mollis


text: OLivier

text: public

text: Maurin, O. 1179, (BNRH)

text: Terminalia phanerophlebia

text: Terminalia phanerophlebia

text: Terminalia phanerophlebia


text: OLivier

text: public

text: Archer, R.H. 3604, (PRE); CANB727446

text: Terminalia prunioides

text: Terminalia prunioides

text: Terminalia prunioides


text: OLivier

text: public

text: Hutchison, P.C. ;2849, (BISH); BISH10005

text: Combretum grandiflorum

text: Combretum grandiflorum

text: Combretum grandiflorum


text: OLivier

text: public

text: Diallo, A. 63, (K); K001286915

text: Combretum micranthum

text: Combretum micranthum

text: Combretum micranthum


text: OLivier

text: public

text: Phillipson, P.B. 5866, (K)

text: Meiostemon humbertii

text: Meiostemon humbertii

text: Combretum humbertii


text: OLivier

text: public

text: Maurin, O. 18, (JRAU)

text: Terminalia brachystemma

text: Terminalia brachystemma

text: Terminalia brachystemma


text: OLivier

text: public

text: Annable, C.R. 3718, (BISH); BISH 661943

text: Terminalia ivorensis

text: Terminalia ivorensis

text: Terminalia ivorensis


text: OLivier

text: public

text: Maurin, O. 79, (JRAU)

text: Terminalia kaernbachii

text: Terminalia kaernbachii

text: Terminalia kaernbachii


text: OLivier

text: public

text: Miller, J.S. 7911, (BISH); BISH 630256

text: Terminalia litoralis

text: Terminalia litoralis

text: Terminalia litoralis


text: OLivier

text: public

text: Lyon, H.S. HBG-83-0706, (BISH); BISH 17197 / 47197

text: Terminalia myriocarpa

text: Terminalia myriocarpa

text: Terminalia myriocarpa


text: OLivier

text: public

text: Maurin, O. 2392, (BNRH)

text: Terminalia sambesiaca

text: Terminalia sambesiaca

text: Terminalia sambesiaca


text: OLivier

text: public

text: Maurin, O. 4164, (BNRH)

text: Terminalia stenostachya

text: Terminalia stenostachya

text: Terminalia stenostachya


text: OLivier

text: public

text: Maurin, O. 4168, (BNRH)

text: Terminalia stuhlmannii

text: Terminalia stuhlmannii

text: Terminalia stuhlmannii


text: OLivier

text: public

text: Liesner,130, (K)

text: Terminalia oblonga

text: Terminalia oblonga

text: Terminalia oblonga


text: OLivier

text: public

text: Maurin, O. 997, (JRAU)

text: Combretum stylesii

text: Combretum stylesii

text: Combretum stylesii


text: OLivier

text: public

text: Ikechukwu, A. 84, (UNIPORT)

text: Terminalia superba

text: Terminalia superba

text: Terminalia superba


text: OLivier

text: public

text: Cooney, B. s.n., (CBG); CBG9213686

text: Terminalia microcarpa

text: Terminalia microcarpa

text: Terminalia microcarpa


text: OLivier

text: public

text: Maurin, O. 2114, (BNRH)

text: Terminalia randii

text: Terminalia randii

text: Terminalia randii


text: OLivier

text: public

text: Fell, D.G. 193, (BRI); AQ0855507

text: Terminalia sericocarpa

text: Terminalia sericocarpa

text: Terminalia sericocarpa


text: OLivier

text: public

text: Simaga, J.M. 1454, (CANB); CANB496302

text: Ehrharta ramosa

text: Ehrharta ramosa

text: Terminalia calamansanay


text: OLivier

text: public

text: Gorts, A.R.A. 482, (K)

text: Combretum cacoucia

text: Combretum cacoucia

text: Combretum cacoucia


text: OLivier

text: public

text: van Andel, T.R. 5192, (K)

text: Combretum laxum

text: Combretum laxum

text: Combretum laxum


text: OLivier

text: public

text: Van Slageren M. 696, (K)

text: Combretum nigricans

text: Combretum nigricans

text: Combretum nigricans


text: OLivier

text: public

text: Maurin, O. 3982, (BNRH)

text: Combretum umbricola

text: Combretum umbricola

text: Combretum umbricola


text: OLivier

text: public

text: Addicott, E.P. 948, (BRI); AQ0550702

text: Terminalia canescens

text: Terminalia canescens

text: Terminalia canescens


text: OLivier

text: public

text: Kemp, J. 11672, (BRI); AQ0794679

text: Terminalia melanocarpa

text: Terminalia melanocarpa

text: Terminalia melanocarpa


text: OLivier

text: public

text: Kemp, J. 6476, (BRI)AQ0618312

text: Terminalia platyptera

text: Terminalia platyptera

text: Terminalia platyptera


text: OLivier

text: public

text: Dorr, L.J. 4128, (K)

text: Terminalia ulexoides

text: Terminalia ulexoides

text: Terminalia ulexoides


text: OLivier

text: public

text: Nicholson, N. 2760, (BRI); AQ0792090

text: Macropteranthes leiocaulis

text: Macropteranthes leiocaulis

text: Macropteranthes leiocaulis


text: OLivier

text: public

text: Leach, G. 4318, (BRI); AQ0531896

text: Macropteranthes kekwickii

text: Macropteranthes kekwickii

text: Macropteranthes kekwickii


text: OLivier

text: public

text: Niyomdham, C. 1453, (K)

text: Combretum tetralophum

text: Combretum tetralophum

text: Combretum tetralophum


text: OLivier

text: public

text: Hyland, B. 9701, (K)

text: Terminalia arenicola

text: Terminalia arenicola

text: Terminalia arenicola


text: OLivier

text: public

text: Breteler, F.J. 14637, (K)

text: Strephonema sericeum

text: Strephonema sericeum

text: Strephonema sericeum


text: OLivier

text: public

text: Coradin L. 5722, (K); K000276499

text: Combretum leprosum

text: Combretum leprosum

text: Combretum leprosum


text: OLivier

text: public

text: Cumming, R.J. 23583, (AQ); AQ0872920

text: Terminalia platyphylla

text: Terminalia platyphylla

text: Terminalia platyphylla


text: JOHN INNES CENTRE, NORWICH, UK.

text: public

tag: Namespace:JOHN INNES CENTRE, NORWICH, UK.

text: 14 days

characteristic: age

text: bread wheat

text: Cadenza

characteristic: cultivar

text: control

text: seedling

text: Ambient

characteristic: environmental stress

text: Cadenza

characteristic: genotype

text: 12 h light / 12 h darkness at 22 deg C, 200 umol m-2 s-1 of LED white light, + 24 h constant light, sampling in constant light

characteristic: growth condition

text: Triticum aestivum

characteristic: ploidy

characteristic: replicate

characteristic: sampling time point

text: Triticum aestivum

characteristic: time


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: JOHN INNES CENTRE, NORWICH, UK.

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 14 days

text: bread wheat

text: Cadenza

text: 3 h chilling (4 deg C), commencing at ZT25

text: seedling

text: 3h chilling at 4 deg C

text: Cadenza

text: Triticum aestivum

characteristic: organism

text: Triticum aestivum

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: JOHN INNES CENTRE, NORWICH, UK.

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: 14 days

text: bread wheat

characteristic: common name

text: Cadenza

text: 3 h chilling (4 deg C), commencing at ZT29

characteristic: description

text: seedling

text: 3h chilling at 4 deg C

text: Cadenza

text: Triticum aestivum

text: Triticum aestivum


text: JOHN INNES CENTRE, NORWICH, UK.

text: public

text: 14 days

characteristic: age

text: bread wheat

text: Cadenza

characteristic: cultivar

text: 3 h chilling (4 deg C), commencing at ZT33

text: seedling

text: 3h chilling at 4 deg C

characteristic: environmental stress

text: Cadenza

characteristic: genotype

characteristic: growth condition

text: Triticum aestivum

characteristic: ploidy

characteristic: replicate

text: Triticum aestivum


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: JOHN INNES CENTRE, NORWICH, UK.

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 14 days

text: bread wheat

text: Cadenza

text: 3 h chilling (4 deg C), commencing at ZT37

text: seedling

text: 3h chilling at 4 deg C

text: Cadenza

text: Triticum aestivum

characteristic: sampling time point

text: Triticum aestivum

characteristic: time


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: JOHN INNES CENTRE, NORWICH, UK.

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: 14 days

text: bread wheat

text: Cadenza

text: 3 h chilling (4 deg C), commencing at ZT41

text: seedling

text: 3h chilling at 4 deg C

text: Cadenza

text: Triticum aestivum

characteristic: organism

text: Triticum aestivum

characteristic: title


text: JOHN INNES CENTRE, NORWICH, UK.

text: public

text: 14 days

characteristic: age

text: bread wheat

characteristic: common name

text: Cadenza

characteristic: cultivar

text: 3 h chilling (4 deg C), commencing at ZT45

characteristic: description

text: seedling

text: 3h chilling at 4 deg C

characteristic: environmental stress

text: Cadenza

characteristic: genotype

characteristic: growth condition

text: Triticum aestivum

text: Triticum aestivum


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Nutricia Research

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Nutricia Research

characteristic: Submitter Id

text: digestive tract environment

characteristic: broad-scale environmental context

characteristic: collection date

text: human

text: feces

characteristic: environmental medium

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: intestine environment

characteristic: local environmental context

text: Homo sapiens

characteristic: project name

text: Homo sapiens

text: Illumina MiSeq

characteristic: sequencing method


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Nutricia Research

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: digestive tract environment

text: human

characteristic: common name

characteristic: description

text: feces

text: Thailand

text: intestine environment

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Illumina MiSeq

characteristic: title