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Swedish red polled 3

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tag: Namespace:IFEVA (CONICET-UBA) Facultad de Agronomia Universidad de Buenos Aires (UBA)

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text: Protocols: Samples were harvested from 42 day-old plants at the beginning of the day. Three biological replicates, each consisting of a pool of leaves from six plants (the third completely expanded leaf), were collected. S. tuberosum var. Spunta wild type plants were used. Sprouted tubers of similar size were planted in pots of 1000 cm3. Plants were grown in a greenhouse with natural radiation (PAR = 500 mmol m-2 s-1) with temperatures ranging between 20°C and 24°C. Experiments were carried out between March and October with a photoperiod of about 10.5 h of light. *For shade avoidance experiments, plants were grown in a greenhouse with natural radiation for 28 days. Then, plants were transferred to simulated shade provided by the same light sources in combination with two green acetate filters (no. 089; LEE Filters, Hampshire, UK) to reduce blue and red light and the R:FR (red to far red) ratio, or kept in control conditions, for additional 14 days. *For drought stress treatment, plants were watered daily at field capacity for 28 days. Then, water was restricted to 35% of field capacity for 14 days. Total RNA was extracted using the Spectrum™ Plant Total RNA Kit (Sigma-Aldrich), according to the manufacturer’s instructions. Unstranded libraries were prepared using standard Illumina kit

text: sporophyte vegetative stage

characteristic: developmental stage

characteristic: environmental stress

text: BBX21-OE CH2

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text: green house study

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text: not applicable

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text: Sterile faecal filtrate transplantation alters phage-microbe dynamics in individuals with metabolic syndrome

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