characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: uncultured Candidatus Saccharibacteria bacterium

characteristic: organism

text: Antibiotic overuse has promoted the spread of antimicrobial resistance (AMR) with significant health and economic consequences. Surveillance of resistance reservoirs, like the rarely explored oral microbiome, is necessary to combat AMR. We characterised the development of the paediatric oral resistome and investigated its role in dental caries in 221 twin children sampled over the first decade of life. From 535 oral metagenomes, we identified 309 antimicrobial resistance genes (ARGs), which significantly clustered by age, with the influence of host genetics on antimicrobial resistance increasing over time. Our results suggest the mobilisation potential of ARGs increased with age as Tn916 transposase was co-located with more species and ARGs as children grew. Dental caries compared to health was associated with a depletion of ARGs and species, which was reversed when restored. Our results highlight the dynamic nature of the paediatric oral resistome, and its potential role in transmission of AMR and dysbiosis.

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Candidatus Saccharibacteria;c__Candidatus Saccharimonia;o__Candidatus Nanosynbacterales;f__Candidatus Nanosynbacteraceae;g__Candidatus Nanosynbacter;s__

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968775_bin.34_MetaWRAP_v1.3_MAG


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaspades v3.15.3

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968775 of study ERP139515.

text: plaque biofilm

characteristic: environmental medium

text: Australia

unit: DD

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: uncultured Capnocytophaga sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Capnocytophaga sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Flavobacteriia;o__Flavobacteriales;f__Flavobacteriaceae;g__Capnocytophaga;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968775_bin.43_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: Campylobacter concisus

characteristic: organism

text: Campylobacter concisus

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Epsilonproteobacteria;o__Campylobacterales;f__Campylobacteraceae;g__Campylobacter;s__Campylobacter concisus

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968775_bin.7_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968776 of study ERP139515.

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: uncultured Streptococcus sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Streptococcus sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Streptococcus;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968776_bin.1_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: uncultured Neisseria sp.

characteristic: organism

text: uncultured Neisseria sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Betaproteobacteria;o__Neisseriales;f__Neisseriaceae;g__Neisseria;s__

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968776_bin.8_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: uncultured Prevotella sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Prevotella sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Prevotellaceae;g__Prevotella;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968776_bin.9_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968777 of study ERP139515.

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: uncultured Campylobacter sp.

characteristic: organism

text: Antibiotic overuse has promoted the spread of antimicrobial resistance (AMR) with significant health and economic consequences. Surveillance of resistance reservoirs, like the rarely explored oral microbiome, is necessary to combat AMR. We characterised the development of the paediatric oral resistome and investigated its role in dental caries in 221 twin children sampled over the first decade of life. From 535 oral metagenomes, we identified 309 antimicrobial resistance genes (ARGs), which significantly clustered by age, with the influence of host genetics on antimicrobial resistance increasing over time. Our results suggest the mobilisation potential of ARGs increased with age as Tn916 transposase was co-located with more species and ARGs as children grew. Dental caries compared to health was associated with a depletion of ARGs and species, which was reversed when restored. Our results highlight the dynamic nature of the paediatric oral resistome, and its potential role in transmission of AMR and dysbiosis.

text: uncultured Campylobacter sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Epsilonproteobacteria;o__Campylobacterales;f__Campylobacteraceae;g__Campylobacter;s__

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968777_bin.16_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaspades v3.15.3

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: uncultured Centipeda sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Centipeda sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Negativicutes;o__Selenomonadales;f__Selenomonadaceae;g__Centipeda;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968777_bin.18_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: Alloprevotella tannerae

characteristic: organism

text: Alloprevotella tannerae

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Prevotellaceae;g__Alloprevotella;s__Alloprevotella tannerae

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968777_bin.21_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: uncultured Streptococcus sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Streptococcus sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968777_bin.3_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: uncultured Veillonella sp.

characteristic: organism

text: uncultured Veillonella sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Negativicutes;o__Veillonellales;f__Veillonellaceae;g__Veillonella;s__

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968777_bin.33_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: uncultured Alloprevotella sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Alloprevotella sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Prevotellaceae;g__Alloprevotella;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968777_bin.5_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: Fusobacterium massiliense

characteristic: organism

text: Fusobacterium massiliense

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Fusobacteria;c__Fusobacteriia;o__Fusobacteriales;f__Fusobacteriaceae;g__Fusobacterium;s__Fusobacterium massiliense

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968777_bin.6_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968777 of study ERP139515.

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: uncultured Candidatus Saccharibacteria bacterium

characteristic: project name

characteristic: sample derived from

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Candidatus Saccharibacteria;c__Candidatus Saccharimonia;o__Candidatus Nanosynbacterales;f__Candidatus Nanosynbacteraceae;g__Candidatus Nanosynbacter;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968777_bin.7_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968778 of study ERP139515.

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: uncultured Streptobacillus sp.

characteristic: organism

text: Antibiotic overuse has promoted the spread of antimicrobial resistance (AMR) with significant health and economic consequences. Surveillance of resistance reservoirs, like the rarely explored oral microbiome, is necessary to combat AMR. We characterised the development of the paediatric oral resistome and investigated its role in dental caries in 221 twin children sampled over the first decade of life. From 535 oral metagenomes, we identified 309 antimicrobial resistance genes (ARGs), which significantly clustered by age, with the influence of host genetics on antimicrobial resistance increasing over time. Our results suggest the mobilisation potential of ARGs increased with age as Tn916 transposase was co-located with more species and ARGs as children grew. Dental caries compared to health was associated with a depletion of ARGs and species, which was reversed when restored. Our results highlight the dynamic nature of the paediatric oral resistome, and its potential role in transmission of AMR and dysbiosis.

text: uncultured Streptobacillus sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Fusobacteria;c__Fusobacteriia;o__Fusobacteriales;f__Leptotrichiaceae;g__Streptobacillus;s__

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968778_bin.13_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: uncultured Campylobacter sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Campylobacter sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Epsilonproteobacteria;o__Campylobacterales;f__Campylobacteraceae;g__Campylobacter;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968778_bin.2_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968779 of study ERP139515.

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: uncultured Porphyromonas sp.

characteristic: organism

text: uncultured Porphyromonas sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Porphyromonadaceae;g__Porphyromonas;s__

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968779_bin.2_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: uncultured Campylobacter sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Campylobacter sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968779_bin.8_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968780 of study ERP139515.

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: Capnocytophaga sputigena

characteristic: organism

text: Capnocytophaga sputigena

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Flavobacteriia;o__Flavobacteriales;f__Flavobacteriaceae;g__Capnocytophaga;s__Capnocytophaga sputigena

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968780_bin.1_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968781 of study ERP139515.

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: uncultured Pseudomonas sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Pseudomonas sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968781_bin.12_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: uncultured Peptostreptococcus sp.

characteristic: organism

text: uncultured Peptostreptococcus sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Eubacteriales;f__Peptostreptococcaceae;g__Peptostreptococcus;s__

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968781_bin.9_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968782 of study ERP139515.

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: uncultured Streptococcus sp.

text: Antibiotic overuse has promoted the spread of antimicrobial resistance (AMR) with significant health and economic consequences. Surveillance of resistance reservoirs, like the rarely explored oral microbiome, is necessary to combat AMR. We characterised the development of the paediatric oral resistome and investigated its role in dental caries in 221 twin children sampled over the first decade of life. From 535 oral metagenomes, we identified 309 antimicrobial resistance genes (ARGs), which significantly clustered by age, with the influence of host genetics on antimicrobial resistance increasing over time. Our results suggest the mobilisation potential of ARGs increased with age as Tn916 transposase was co-located with more species and ARGs as children grew. Dental caries compared to health was associated with a depletion of ARGs and species, which was reversed when restored. Our results highlight the dynamic nature of the paediatric oral resistome, and its potential role in transmission of AMR and dysbiosis.

characteristic: project name

characteristic: sample derived from

text: uncultured Streptococcus sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Streptococcus;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968782_bin.20_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968783 of study ERP139515.

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: uncultured Propionibacterium sp.

characteristic: organism

text: uncultured Propionibacterium sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Actinobacteria;c__Actinomycetia;o__Propionibacteriales;f__Propionibacteriaceae;g__Propionibacterium;s__

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968783_bin.15_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: uncultured Prevotella sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Prevotella sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Prevotellaceae;g__Prevotella;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968783_bin.17_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: uncultured Actinomyces sp.

characteristic: organism

text: uncultured Actinomyces sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Actinobacteria;c__Actinomycetia;o__Actinomycetales;f__Actinomycetaceae;g__Actinomyces;s__

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968783_bin.29_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: Streptococcus sanguinis

characteristic: project name

characteristic: sample derived from

text: Streptococcus sanguinis

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Streptococcus;s__Streptococcus sanguinis

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968783_bin.4_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968784 of study ERP139515.

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: uncultured Erwinia sp.

characteristic: organism

text: uncultured Erwinia sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Erwiniaceae;g__Erwinia;s__

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968784_bin.9_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968785 of study ERP139515.

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: Acinetobacter baylyi

text: Antibiotic overuse has promoted the spread of antimicrobial resistance (AMR) with significant health and economic consequences. Surveillance of resistance reservoirs, like the rarely explored oral microbiome, is necessary to combat AMR. We characterised the development of the paediatric oral resistome and investigated its role in dental caries in 221 twin children sampled over the first decade of life. From 535 oral metagenomes, we identified 309 antimicrobial resistance genes (ARGs), which significantly clustered by age, with the influence of host genetics on antimicrobial resistance increasing over time. Our results suggest the mobilisation potential of ARGs increased with age as Tn916 transposase was co-located with more species and ARGs as children grew. Dental caries compared to health was associated with a depletion of ARGs and species, which was reversed when restored. Our results highlight the dynamic nature of the paediatric oral resistome, and its potential role in transmission of AMR and dysbiosis.

characteristic: project name

characteristic: sample derived from

text: Acinetobacter baylyi

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter;s__Acinetobacter baylyi

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968785_bin.1_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: Acinetobacter bereziniae

characteristic: organism

text: Acinetobacter bereziniae

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter;s__Acinetobacter bereziniae

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968785_bin.11_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: Oribacterium asaccharolyticum

characteristic: project name

characteristic: sample derived from

text: Oribacterium asaccharolyticum

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Eubacteriales;f__Lachnospiraceae;g__Oribacterium;s__Oribacterium asaccharolyticum

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968785_bin.12_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: uncultured Candidatus Saccharibacteria bacterium

characteristic: organism

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Candidatus Saccharibacteria;c__;o__;f__;g__;s__

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968785_bin.19_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: uncultured Comamonas sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Comamonas sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Betaproteobacteria;o__Burkholderiales;f__Comamonadaceae;g__Comamonas;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968785_bin.27_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: Acinetobacter soli

characteristic: organism

text: Acinetobacter soli

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter;s__Acinetobacter soli

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968785_bin.31_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968785 of study ERP139515.

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: uncultured Acinetobacter sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Acinetobacter sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968785_bin.33_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: Pseudomonas protegens

characteristic: organism

text: Antibiotic overuse has promoted the spread of antimicrobial resistance (AMR) with significant health and economic consequences. Surveillance of resistance reservoirs, like the rarely explored oral microbiome, is necessary to combat AMR. We characterised the development of the paediatric oral resistome and investigated its role in dental caries in 221 twin children sampled over the first decade of life. From 535 oral metagenomes, we identified 309 antimicrobial resistance genes (ARGs), which significantly clustered by age, with the influence of host genetics on antimicrobial resistance increasing over time. Our results suggest the mobilisation potential of ARGs increased with age as Tn916 transposase was co-located with more species and ARGs as children grew. Dental caries compared to health was associated with a depletion of ARGs and species, which was reversed when restored. Our results highlight the dynamic nature of the paediatric oral resistome, and its potential role in transmission of AMR and dysbiosis.

text: Pseudomonas protegens

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas;s__Pseudomonas protegens

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968785_bin.37_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968786 of study ERP139515.

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: Leptotrichia wadei

characteristic: project name

characteristic: sample derived from

text: Leptotrichia wadei

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Fusobacteria;c__Fusobacteriia;o__Fusobacteriales;f__Leptotrichiaceae;g__Leptotrichia;s__Leptotrichia wadei

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968786_bin.26_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968787 of study ERP139515.

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: uncultured Sphingomonas sp.

characteristic: organism

text: uncultured Sphingomonas sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Sphingomonadales;f__Sphingomonadaceae;g__Sphingomonas;s__

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968787_bin.2_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: Empedobacter tilapiae

characteristic: project name

characteristic: sample derived from

text: Empedobacter tilapiae

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Flavobacteriia;o__Flavobacteriales;f__Weeksellaceae;g__Empedobacter;s__Empedobacter tilapiae

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968787_bin.3_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: Klebsiella variicola

characteristic: organism

text: Klebsiella variicola

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Klebsiella;s__Klebsiella variicola

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968787_bin.8_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968788 of study ERP139515.

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: uncultured Candidatus Saccharimonas sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Candidatus Saccharimonas sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Candidatus Saccharibacteria;c__;o__;f__;g__Candidatus Saccharimonas;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968788_bin.3_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: uncultured Candidatus Saccharibacteria bacterium

characteristic: organism

text: Antibiotic overuse has promoted the spread of antimicrobial resistance (AMR) with significant health and economic consequences. Surveillance of resistance reservoirs, like the rarely explored oral microbiome, is necessary to combat AMR. We characterised the development of the paediatric oral resistome and investigated its role in dental caries in 221 twin children sampled over the first decade of life. From 535 oral metagenomes, we identified 309 antimicrobial resistance genes (ARGs), which significantly clustered by age, with the influence of host genetics on antimicrobial resistance increasing over time. Our results suggest the mobilisation potential of ARGs increased with age as Tn916 transposase was co-located with more species and ARGs as children grew. Dental caries compared to health was associated with a depletion of ARGs and species, which was reversed when restored. Our results highlight the dynamic nature of the paediatric oral resistome, and its potential role in transmission of AMR and dysbiosis.

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Candidatus Saccharibacteria;c__;o__;f__;g__;s__

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968788_bin.6_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968789 of study ERP139515.

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: uncultured Pelosinus sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Pelosinus sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Negativicutes;o__Selenomonadales;f__Sporomusaceae;g__Pelosinus;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968789_bin.10_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: uncultured Streptococcus sp.

characteristic: organism

text: uncultured Streptococcus sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Streptococcus;s__

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968789_bin.11_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: Haemophilus parainfluenzae

characteristic: project name

characteristic: sample derived from

text: Haemophilus parainfluenzae

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pasteurellales;f__Pasteurellaceae;g__Haemophilus;s__Haemophilus parainfluenzae

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968789_bin.8_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968790 of study ERP139515.

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: uncultured Corynebacterium sp.

characteristic: organism

text: uncultured Corynebacterium sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Actinobacteria;c__Actinomycetia;o__Corynebacteriales;f__Corynebacteriaceae;g__Corynebacterium;s__

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968790_bin.6_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968792 of study ERP139515.

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: uncultured Rahnella sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Rahnella sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Yersiniaceae;g__Rahnella;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968792_bin.12_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: Pseudomonas kuykendallii

characteristic: organism

text: Antibiotic overuse has promoted the spread of antimicrobial resistance (AMR) with significant health and economic consequences. Surveillance of resistance reservoirs, like the rarely explored oral microbiome, is necessary to combat AMR. We characterised the development of the paediatric oral resistome and investigated its role in dental caries in 221 twin children sampled over the first decade of life. From 535 oral metagenomes, we identified 309 antimicrobial resistance genes (ARGs), which significantly clustered by age, with the influence of host genetics on antimicrobial resistance increasing over time. Our results suggest the mobilisation potential of ARGs increased with age as Tn916 transposase was co-located with more species and ARGs as children grew. Dental caries compared to health was associated with a depletion of ARGs and species, which was reversed when restored. Our results highlight the dynamic nature of the paediatric oral resistome, and its potential role in transmission of AMR and dysbiosis.

text: Pseudomonas kuykendallii

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas;s__Pseudomonas kuykendallii

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968792_bin.17_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: Actinomyces naeslundii

characteristic: project name

characteristic: sample derived from

text: Actinomyces naeslundii

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Actinobacteria;c__Actinomycetia;o__Actinomycetales;f__Actinomycetaceae;g__Actinomyces;s__Actinomyces naeslundii

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968792_bin.7_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968793 of study ERP139515.

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: uncultured Enterobacteriaceae bacterium

characteristic: organism

text: uncultured Enterobacteriaceae bacterium

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Superficieibacter;s__

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968793_bin.15_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: uncultured Weeksellaceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Weeksellaceae bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Flavobacteriia;o__Flavobacteriales;f__Weeksellaceae;g__Epilithonimonas;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968793_bin.17_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: uncultured Streptococcus sp.

characteristic: organism

text: uncultured Streptococcus sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Streptococcus;s__

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968793_bin.7_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968794 of study ERP139515.

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: uncultured Prevotellaceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Prevotellaceae bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Prevotellaceae;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968794_bin.8_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968795 of study ERP139515.

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: uncultured Atopobium sp.

characteristic: organism

text: Antibiotic overuse has promoted the spread of antimicrobial resistance (AMR) with significant health and economic consequences. Surveillance of resistance reservoirs, like the rarely explored oral microbiome, is necessary to combat AMR. We characterised the development of the paediatric oral resistome and investigated its role in dental caries in 221 twin children sampled over the first decade of life. From 535 oral metagenomes, we identified 309 antimicrobial resistance genes (ARGs), which significantly clustered by age, with the influence of host genetics on antimicrobial resistance increasing over time. Our results suggest the mobilisation potential of ARGs increased with age as Tn916 transposase was co-located with more species and ARGs as children grew. Dental caries compared to health was associated with a depletion of ARGs and species, which was reversed when restored. Our results highlight the dynamic nature of the paediatric oral resistome, and its potential role in transmission of AMR and dysbiosis.

text: uncultured Atopobium sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Actinobacteria;c__Coriobacteriia;o__Coriobacteriales;f__Atopobiaceae;g__Atopobium;s__

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968795_bin.11_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: Aeromonas caviae

characteristic: project name

characteristic: sample derived from

text: Aeromonas caviae

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Aeromonadales;f__Aeromonadaceae;g__Aeromonas;s__Aeromonas caviae

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968795_bin.22_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968796 of study ERP139515.

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: uncultured Serratia sp.

characteristic: organism

text: uncultured Serratia sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Yersiniaceae;g__Serratia;s__

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968796_bin.10_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: Carnobacterium divergens

characteristic: project name

characteristic: sample derived from

text: Carnobacterium divergens

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Carnobacteriaceae;g__Carnobacterium;s__Carnobacterium divergens

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968796_bin.16_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: uncultured Flavobacteriaceae bacterium

characteristic: organism

text: uncultured Flavobacteriaceae bacterium

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Flavobacteriia;o__Flavobacteriales;f__Flavobacteriaceae;g__;s__

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968796_bin.18_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: Haemophilus parainfluenzae

characteristic: project name

characteristic: sample derived from

text: Haemophilus parainfluenzae

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pasteurellales;f__Pasteurellaceae;g__Haemophilus;s__Haemophilus parainfluenzae

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968796_bin.20_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: uncultured Porphyromonadaceae bacterium

characteristic: organism

text: uncultured Porphyromonadaceae bacterium

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Porphyromonadaceae;g__;s__

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968796_bin.6_MetaWRAP_v1.3_MAG