characteristic: ENA-CHECKLIST

text: UK Health Security Agency

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:179-5722

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis

characteristic: title


status: PUBLIC

tag: attribute

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UK Health Security Agency

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:179-5723

tag: Namespace:UK Health Security Agency

characteristic: Submitter Id

characteristic: collection date

text: Invasive isolate

characteristic: description

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: diseased

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

characteristic: isolate

text: invasive

text: Neisseria meningitidis

characteristic: organism

text: Neisseria meningitidis


characteristic: ENA-CHECKLIST

text: UK Health Security Agency

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:179-5724

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UK Health Security Agency

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:179-5725

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis


characteristic: External Id

text: UK Health Security Agency

characteristic: INSDC center name

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:180-5726

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis


text: UK Health Security Agency

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:180-5727

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis


text: UK Health Security Agency

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:180-5728

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis


text: UK Health Security Agency

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:180-5729

characteristic: Submitter Id

characteristic: collection date

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis


text: UK Health Security Agency

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:180-5730

text: Invasive isolate

characteristic: description

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: diseased

text: Homo sapiens

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis


text: UK Health Security Agency

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:180-5731

text: Invasive isolate

text: United Kingdom

text: diseased

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis


text: UK Health Security Agency

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:180-5732

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

characteristic: isolate

text: invasive

text: Neisseria meningitidis

characteristic: organism

text: Neisseria meningitidis


characteristic: ENA-CHECKLIST

text: UK Health Security Agency

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:180-5733

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UK Health Security Agency

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:180-5734

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis


characteristic: External Id

text: UK Health Security Agency

characteristic: INSDC center name

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:180-5735

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis


text: UK Health Security Agency

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:180-5736

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis


text: UK Health Security Agency

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:180-5737

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis


text: UK Health Security Agency

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:180-5738

characteristic: Submitter Id

characteristic: collection date

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis


text: UK Health Security Agency

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:180-5739

text: Invasive isolate

characteristic: description

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: diseased

text: Homo sapiens

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis


text: UK Health Security Agency

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:180-5740

text: Invasive isolate

text: United Kingdom

text: diseased

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis


text: UK Health Security Agency

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:180-5741

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

characteristic: isolate

text: invasive

text: Neisseria meningitidis

characteristic: organism

text: Neisseria meningitidis


characteristic: ENA-CHECKLIST

text: UK Health Security Agency

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:180-5742

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UK Health Security Agency

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:180-5743

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis


characteristic: External Id

text: UK Health Security Agency

characteristic: INSDC center name

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:180-5744

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis


text: UK Health Security Agency

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:180-5745

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis


text: UK Health Security Agency

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:180-5746

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis


text: UK Health Security Agency

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:181-5747

characteristic: Submitter Id

characteristic: collection date

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis


text: UK Health Security Agency

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:181-5748

text: Invasive isolate

characteristic: description

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: diseased

text: Homo sapiens

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis


text: UK Health Security Agency

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:181-5749

text: Invasive isolate

text: United Kingdom

text: diseased

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis


text: UK Health Security Agency

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:181-5750

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

characteristic: isolate

text: invasive

text: Neisseria meningitidis

characteristic: organism

text: Neisseria meningitidis


characteristic: ENA-CHECKLIST

text: UK Health Security Agency

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:181-5751

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UK Health Security Agency

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:181-5752

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis


characteristic: External Id

text: UK Health Security Agency

characteristic: INSDC center name

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:181-5753

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis


text: UK Health Security Agency

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:181-5754

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis


text: UK Health Security Agency

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:181-5755

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis


text: UK Health Security Agency

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:181-5756

characteristic: Submitter Id

characteristic: collection date

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis


text: UK Health Security Agency

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:181-5757

text: Invasive isolate

characteristic: description

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: diseased

text: Homo sapiens

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis


text: UK Health Security Agency

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:181-5758

text: Invasive isolate

text: United Kingdom

text: diseased

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis


text: UK Health Security Agency

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:181-5759

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

characteristic: isolate

text: invasive

text: Neisseria meningitidis

characteristic: organism

text: Neisseria meningitidis


characteristic: ENA-CHECKLIST

text: UK Health Security Agency

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:181-5760

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UK Health Security Agency

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:181-5761

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis


characteristic: External Id

text: UK Health Security Agency

characteristic: INSDC center name

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:181-5762

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis


text: UK Health Security Agency

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:181-5763

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis


text: UK Health Security Agency

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:181-5764

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis


text: UK Health Security Agency

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:181-5765

characteristic: Submitter Id

characteristic: collection date

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis


text: UK Health Security Agency

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:181-5766

text: Invasive isolate

characteristic: description

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: diseased

text: Homo sapiens

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis


text: UK Health Security Agency

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:181-5767

text: Invasive isolate

text: United Kingdom

text: diseased

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis


text: UK Health Security Agency

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:182-5768

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

characteristic: isolate

text: invasive

text: Neisseria meningitidis

characteristic: organism

text: Neisseria meningitidis


characteristic: ENA-CHECKLIST

text: UK Health Security Agency

text: public

text: ena-SAMPLE-TAB-01-08-2023-12:09:31:182-5769

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: James Hutton Institute

text: public

tag: Namespace:James Hutton Institute

text: potato

characteristic: common name

text: Illumina RNASeq of DM

text: United Kingdom

text: Solanum tuberosum

text: Solanum tuberosum


characteristic: External Id

text: James Hutton Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: potato

text: Illumina RenSeq_PenSeq of DM

text: United Kingdom

text: Solanum tuberosum

text: Solanum tuberosum


characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Department of Molecular and Cellular Pathology and Therapy, Instituto de Biomedicina de Valencia (IBV-CSIC)

text: ArrayExpress

characteristic: broker name

text: not collected

text: baker's yeast

text: Protocols: After the respective treatment, cells were collected by centrifugation, washed with ice-cold water, and frozen as 500mg aliquots at -80ºC. SATAY was performed as described Michel, A.H and Kornmann, B. (2022 Yeast Functional Genomics. Methods in Molecular Biology, vol 2477), with some modifications. ByK352 hfd1∆::KanMX strain was transformed with pBK549 plasmid containing the transposon, obtained from the Kornmann Lab (Michel & Kornmann, 2022). Transformed cells were inoculated into 200mL of SC liquid + 0.2% Glucose + 2% Raffinose -Uracil at OD600 of 0.15 and the culture was grown until saturation (OD600 3.9) for 16 hours. Then, the culture was concentrated by centrifugation to obtain a final OD600 of 39. From this culture, 200µL were plated on 50x8.5cm plates containing 25mL of SC + 2% Galactose -Adenine using glass beads. Plates were incubated for 3 weeks at 30ºC. After incubation clones reached a density on the plate of 46750 colonies/plate, on average, with a total number of 2.3E+06 generated mutants. All colonies were then scrapped with very low volume of sterile water and pooled together. From the pool, two independent cultures of 1L were inoculated in SC + 2%Glucose -Adenine. The control one was inoculated at an initial OD600 of 0.2 and grown until saturation (OD600 of 8) at 30ºC for 36 hours. The other culture, used for t-2-hex treatment, was grown for 36 hours from an initial OD600 of 0.05 to 3, and then diluted to OD600 0.1 in 500mL of SC +2% Glucose -Adenine treated with 50µM of t-2-hex (Avanti Polar Lipids). After growing for 32 hours to OD600 1.1, it was diluted again to OD600 0.1 in 500mL SC +2% Glucose -Adenine + 50µM of t-2-hex and grown for 24 hours to saturation (OD600 2.9). A 500mg cell pellet was suspended in 500µL of Cell Breaking Buffer (consisting of 2% Triton X-100, 1% SDS, 100 mM NaCl, 100 mM Tris-HCl pH 8.0, 1 mM EDTA) and divided into 280µL aliquots. To each aliquot, 200µL of Phenol:Chloroform:Isoamyl alcohol (25:25:1) and 300µL of unwashed glass beads (0.5 mm) were added. Cells were lysed by subjecting them to 10 cycles of vortexing for 30 seconds followed by incubation on ice for 30 seconds. Next, 200µL of TE buffer was added to each lysate, which was then centrifuged at 16100g for 5 minutes at 4˚C. The upper layer (approximately 400µL) was carefully transferred to a new tube, and 2.5 volumes of 100% ethanol were added, followed by mixing by inversion. The DNA was pelleted by centrifugation at 16100g for 5 minutes at 20˚C. The supernatant was discarded, and the DNA pellets were resuspended in 200µL of RNAse A solution (250µg/ml) and incubated for 15 minutes at 55˚C with gentle agitation using a thermoblock at 1000rpm. Then, 2.5 volumes of 100% ethanol and 0.1 volume of 3 M NaOAc pH 5.2 were added, and the samples were mixed by inversion. The DNA was pelleted by centrifugation at 16100g for 5 minutes at 20˚C. The pellets were washed with 70% ethanol under the same conditions, the supernatant was completely removed, and the pellets were dried for 10 minutes at 37˚C. Finally, the pellets were resuspended in a total volume of 100 ml of water and incubated for 10 minutes at 55˚C with gentle agitation using a thermoblock at 700rpm. The DNA was then analyzed on a 0.6% 1X TBE agarose gel using a standard 1 kb GeneRuler, and quantification was performed using Fiji software. 2μg of both genomic DNA were digested using 50U of MboI (DpnII) (Thermo Fisher Scientific) and Hin1II (NlaIII) (Thermo Fisher Scientific) enzymes at 37°C for 24h, in separated reactions. Subsequently, the enzymatic reaction was heat-inactivated at 65°C for 20 minutes. The resulting DNA fragments were circularized by adding 25 Weiss U of T4 ligase (Thermo Fisher Scientific) and ligated at 22°C for 6 hours in a total volume of 400µL. To precipitate the circularized DNA molecules, a mixture of 0.3M sodium acetate (pH 5.2), 1mL of ethanol, and 5μg of linear acrylamide (Ambion AM9520) was introduced. The mixture was then incubated at -20°C overnight. After precipitation, the DNA was pelleted at 16100g and 4°C for 20 minutes, followed by washing with 1mL of 70% ethanol. The DNA was then dried at 37°C for 10 minutes. Each prepared circularized DNA sample was resuspended in 100µL of water. For transposon amplification, PCR was performed using the forward primer P5_MiniDs (AATGATACGGCGACCACCGAGATCTACtccgtcccgcaagttaaatatga) and the reverse primer P7_MiniDS (CAAGCAGAAGACGGCATACGAGATNNNNNNNNacgaaaacgaacgggataaatac). Each primer consisted of two parts: the Illumina adaptor (uppercase letters) and a fragment from MiniDs (lowercase letters). The 8-nucleotide index present in P7_MiniDs allows distinction among different libraries. For PCR, circularized DNA was divided into 10 x 100µL reactions containing: 10µL of ligated DNA, 10μL dNTPs mix 2mM each, 10μL 10x DreamTaq Buffer (Thermo Fisher Scientific), 1μL 100uM primer P5, 1uL 100μM primer P7, 2μL DreamTaq DNA Polimerase (5U/μL) (Thermo Fisher Scientific). PCR was performed under the following parameters: 95ºC 5min, 35x [95ºC 30s, 55ºC 30s, 72ºC 3min], 72ºC 10min. Finally, for each condition (Control, t-2-hex) all PCR reactions were pooled into one Hin1II-digested pool and one MboI-digested pool. 100μL from each pool were purified with CleanNGS magnetic beads (CleanNA) according to manufacturer intstructions.

text: hfd1Δ::KanMX ade2∆::HIS3

characteristic: genetic modification

text: MATa; his3∆1; ade2∆::HIS3; leu2∆0; met15∆0; ura3∆0; hfd1Δ::KanMX

characteristic: genotype

text: not collected

text: SC + 2%Glucose -Adenine

characteristic: growth condition

text: not applicable

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: BY4741 hfd1Δ

characteristic: strain

text: ControlDpnII


text: Department of Molecular and Cellular Pathology and Therapy, Instituto de Biomedicina de Valencia (IBV-CSIC)

text: public

characteristic: Submitter Id

text: ArrayExpress

text: not collected

characteristic: collection date

text: baker's yeast

characteristic: description

text: hfd1Δ::KanMX ade2∆::HIS3

text: not collected

characteristic: geographic location (country and/or sea)

text: SC + 2%Glucose -Adenine

text: not applicable

characteristic: isolate

text: Saccharomyces cerevisiae

characteristic: organism

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: BY4741 hfd1Δ

text: ControlNlaIII


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: ArrayExpress

text: not collected

text: baker's yeast

characteristic: common name

text: hfd1Δ::KanMX ade2∆::HIS3

text: not collected

text: SC + 2%Glucose -Adenine

text: not applicable

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: BY4741 hfd1Δ

characteristic: title


text: Streptococcus pneumoniae

characteristic: ArrayExpress-SPECIES

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: restricted access

text: Streptococcus pneumoniae

text: Israel

text: Streptococcus pneumoniae

text: Streptococcus pneumoniae


text: Streptococcus pneumoniae

text: SC

text: public

characteristic: Submitter Id

text: restricted access

characteristic: collection date

text: Streptococcus pneumoniae

text: South Africa

text: Streptococcus pneumoniae

text: Streptococcus pneumoniae


text: Department of Zoology

text: public

tag: Namespace:Department of Zoology

text: Bacteriome

characteristic: description

text: Czech Republic

characteristic: geographic location (country and/or sea)

text: metagenome

text: metagenome

text: House mouse microbiota


text: Department of Zoology

text: public

text: Mycobiome

text: Czech Republic

text: metagenome

characteristic: organism

text: metagenome

text: House mouse microbiota


characteristic: ENA-CHECKLIST

text: James Hutton Institute

text: public

text: potato late blight agent

text: Illumina PenSeq enrichment

text: Ireland

text: Phytophthora infestans

text: Phytophthora infestans


characteristic: ENA-FIRST-PUBLIC

text: James Hutton Institute

text: public

text: potato late blight agent

text: Illumina PenSeq enrichment

text: Netherlands

text: Phytophthora infestans

text: Phytophthora infestans


PP10 0787_PenSeq

characteristic: ENA-LAST-UPDATE

text: James Hutton Institute

text: public

text: PP10 0787_PenSeq

text: potato late blight agent

text: Illumina PenSeq enrichment

text: Netherlands

text: Phytophthora infestans

text: Phytophthora infestans

text: PP10 0787_PenSeq


characteristic: External Id

text: James Hutton Institute

text: public

text: potato late blight agent

text: Illumina PenSeq enrichment

text: USA

text: Phytophthora infestans

text: Phytophthora infestans


text: James Hutton Institute

text: public

text: potato late blight agent

characteristic: common name

text: Illumina PenSeq enrichment

text: Colombia

text: Phytophthora infestans

text: Phytophthora infestans


S. mur#1_PenSeq

text: James Hutton Institute

text: public

text: S. mur#1_PenSeq

text: potato late blight agent

text: Illumina PenSeq enrichment

text: Colombia

text: Phytophthora infestans

text: Phytophthora infestans

text: S. mur#1_PenSeq

characteristic: title


S. phu#13_PenSeq

text: James Hutton Institute

text: public

text: S. phu#13_PenSeq

text: potato late blight agent

text: Illumina PenSeq enrichment

text: Colombia

text: Phytophthora infestans

text: Phytophthora infestans

text: S. phu#13_PenSeq


S. ph#18_PenSeq

text: James Hutton Institute

text: public

text: S. ph#18_PenSeq

text: potato late blight agent

text: Illumina PenSeq enrichment

text: Colombia

text: Phytophthora infestans

text: Phytophthora infestans

text: S. ph#18_PenSeq


S. ph#8_PenSeq

text: James Hutton Institute

text: public

text: S. ph#8_PenSeq

text: potato late blight agent

text: Illumina PenSeq enrichment

text: Colombia

text: Phytophthora infestans

text: Phytophthora infestans

text: S. ph#8_PenSeq


S. tub#18_PenSeq

text: James Hutton Institute

text: public

text: S. tub#18_PenSeq

text: potato late blight agent

text: Illumina PenSeq enrichment

text: Colombia

text: Phytophthora infestans

text: Phytophthora infestans

text: S. tub#18_PenSeq


S. tub#16_PenSeq

text: James Hutton Institute

text: public

text: S. tub#16_PenSeq

text: potato late blight agent

text: Illumina PenSeq enrichment

text: Colombia

text: Phytophthora infestans

text: Phytophthora infestans

text: S. tub#16_PenSeq


text: James Hutton Institute

text: public

text: potato late blight agent

text: Illumina PenSeq enrichment

text: United Kingdom

text: Phytophthora infestans

text: Phytophthora infestans


text: James Hutton Institute

text: public

text: potato late blight agent

text: Illumina PenSeq enrichment

text: China

text: Phytophthora infestans

text: Phytophthora infestans


text: IMEDEA

text: public

text: metagenome of pink-shrimp_T0_DS1

text: Spain

text: fish gut metagenome

text: fish gut metagenome


text: IMEDEA

text: public

text: metagenome of pink-shrimp_T0_DS2

text: Spain

text: fish gut metagenome

text: fish gut metagenome


text: IMEDEA

text: public

text: metagenome of pink-shrimp_T0_DS3

text: Spain

text: fish gut metagenome

text: fish gut metagenome


text: IMEDEA

text: public

text: metagenome of fish gut T0_DS_IAM_1

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T0_DS_IAM_1


text: IMEDEA

text: public

text: metagenome of fish gut T0_DS_IAM_2

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T0_DS_IAM_2


text: IMEDEA

text: public

text: metagenome of fish gut T0_DS_IAM_3

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T0_DS_IAM_3


text: IMEDEA

text: public

text: metagenome of fish gut T0_DS_IAM_4

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T0_DS_IAM_4


text: IMEDEA

text: public

text: metagenome of fish gut T0_DS_IPM_1

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T0_DS_IPM_1


text: IMEDEA

text: public

text: metagenome of fish gut T0_DS_IPM_2

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T0_DS_IPM_2


text: IMEDEA

text: public

text: metagenome of fish gut T0_DS_IPM_3

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T0_DS_IPM_3


text: IMEDEA

text: public

text: metagenome of fish gut T0_DS_IPM_4

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T0_DS_IPM_4


text: IMEDEA

text: public

text: metagenome of comercial_fed_T0_OPT1

text: Spain

text: fish gut metagenome

text: fish gut metagenome


text: IMEDEA

text: public

text: metagenome of comercial_fed_T0_OPT2

text: Spain

text: fish gut metagenome

text: fish gut metagenome


text: IMEDEA

text: public

text: metagenome of comercial_fed_T0_OPT3

text: Spain

text: fish gut metagenome

text: fish gut metagenome


text: IMEDEA

text: public

text: metagenome of fish gut T0_OPT_IAM_1

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T0_OPT_IAM_1


text: IMEDEA

text: public

text: metagenome of fish gut T0_OPT_IAM_2

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T0_OPT_IAM_2


text: IMEDEA

text: public

text: metagenome of fish gut T0_OPT_IAM_3

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T0_OPT_IAM_3


text: IMEDEA

text: public

text: metagenome of fish gut T0_OPT_IAM_4

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T0_OPT_IAM_4


text: IMEDEA

text: public

text: metagenome of fish gut T0_OPT_IPM_1

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T0_OPT_IPM_1


text: IMEDEA

text: public

text: metagenome of fish gut T0_OPT_IPM_2

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T0_OPT_IPM_2


text: IMEDEA

text: public

text: metagenome of fish gut T0_OPT_IPM_3

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T0_OPT_IPM_3


text: IMEDEA

text: public

text: metagenome of fish gut T0_OPT_IPM_4

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T0_OPT_IPM_4


text: IMEDEA

text: public

text: metagenome of pink-shrimp_T1_DS1

text: Spain

text: fish gut metagenome

text: fish gut metagenome


text: IMEDEA

text: public

text: metagenome of pink-shrimp_T1_DS2

text: Spain

text: fish gut metagenome

text: fish gut metagenome


text: IMEDEA

text: public

text: metagenome of pink-shrimp_T1_DS3

text: Spain

text: fish gut metagenome

text: fish gut metagenome


text: IMEDEA

text: public

text: metagenome of fish gut T1_DS_IAM_1

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T1_DS_IAM_1


text: IMEDEA

text: public

text: metagenome of fish gut T1_DS_IAM_2

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T1_DS_IAM_2


text: IMEDEA

text: public

text: metagenome of fish gut T1_DS_IAM_3

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T1_DS_IAM_3


text: IMEDEA

text: public

text: metagenome of fish gut T1_DS_IAM_4

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T1_DS_IAM_4


text: IMEDEA

text: public

text: metagenome of fish gut T1_DS_IAM_5

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T1_DS_IAM_5


text: IMEDEA

text: public

text: metagenome of fish gut T1_DS_IAM_6

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T1_DS_IAM_6


text: IMEDEA

text: public

text: metagenome of fish gut T1_DS_IAM_7

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T1_DS_IAM_7


text: IMEDEA

text: public

text: metagenome of fish gut T1_DS_IAM_8

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T1_DS_IAM_8


text: IMEDEA

text: public

text: metagenome of fish gut T1_DS_IPM_1

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T1_DS_IPM_1


text: IMEDEA

text: public

text: metagenome of fish gut T1_DS_IPM_2

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T1_DS_IPM_2


text: IMEDEA

text: public

text: metagenome of fish gut T1_DS_IPM_3

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T1_DS_IPM_3


text: IMEDEA

text: public

text: metagenome of fish gut T1_DS_IPM_4

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T1_DS_IPM_4


text: IMEDEA

text: public

text: metagenome of fish gut T1_DS_IPM_5

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T1_DS_IPM_5


text: IMEDEA

text: public

text: metagenome of fish gut T1_DS_IPM_6

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T1_DS_IPM_6


text: IMEDEA

text: public

text: metagenome of fish gut T1_DS_IPM_7

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T1_DS_IPM_7


text: IMEDEA

text: public

text: metagenome of fish gut T1_DS_IPM_8

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T1_DS_IPM_8


text: IMEDEA

text: public

text: metagenome of comercial_fed_T1_OPT1

text: Spain

text: fish gut metagenome

text: fish gut metagenome


text: IMEDEA

text: public

text: metagenome of comercial_fed_T1_OPT2

text: Spain

text: fish gut metagenome

text: fish gut metagenome


text: IMEDEA

text: public

text: metagenome of comercial_fed_T1_OPT3

text: Spain

text: fish gut metagenome

text: fish gut metagenome


text: IMEDEA

text: public

text: metagenome of fish gut T1_OPT_IAM_1

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T1_OPT_IAM_1


text: IMEDEA

text: public

text: metagenome of fish gut T1_OPT_IAM_2

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T1_OPT_IAM_2


text: IMEDEA

text: public

text: metagenome of fish gut T1_OPT_IAM_3

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T1_OPT_IAM_3


text: IMEDEA

text: public

text: metagenome of fish gut T1_OPT_IAM_4

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T1_OPT_IAM_4


text: IMEDEA

text: public

text: metagenome of fish gut T1_OPT_IAM_5

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T1_OPT_IAM_5


text: IMEDEA

text: public

text: metagenome of fish gut T1_OPT_IAM_6

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T1_OPT_IAM_6


text: IMEDEA

text: public

text: metagenome of fish gut T1_OPT_IAM_7

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T1_OPT_IAM_7


text: IMEDEA

text: public

text: metagenome of fish gut T1_OPT_IAM_8

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T1_OPT_IAM_8


text: IMEDEA

text: public

text: metagenome of fish gut T1_OPT_IPM_1

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T1_OPT_IPM_1


text: IMEDEA

text: public

text: metagenome of fish gut T1_OPT_IPM_2

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T1_OPT_IPM_2


text: IMEDEA

text: public

text: metagenome of fish gut T1_OPT_IPM_3

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T1_OPT_IPM_3


text: IMEDEA

text: public

text: metagenome of fish gut T1_OPT_IPM_4

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T1_OPT_IPM_4


text: IMEDEA

text: public

text: metagenome of fish gut T1_OPT_IPM_5

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T1_OPT_IPM_5


text: IMEDEA

text: public

text: metagenome of fish gut T1_OPT_IPM_6

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T1_OPT_IPM_6


text: IMEDEA

text: public

text: metagenome of fish gut T1_OPT_IPM_7

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T1_OPT_IPM_7


text: IMEDEA

text: public

text: metagenome of fish gut T1_OPT_IPM_8

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T1_OPT_IPM_8


text: IMEDEA

text: public

text: metagenome of pink-shrimp_T2_DS1

text: Spain

text: fish gut metagenome

text: fish gut metagenome


text: IMEDEA

text: public

text: metagenome of pink-shrimp_T2_DS2

text: Spain

text: fish gut metagenome

text: fish gut metagenome


text: IMEDEA

text: public

text: metagenome of pink-shrimp_T2_DS3

text: Spain

text: fish gut metagenome

text: fish gut metagenome


text: IMEDEA

text: public

text: metagenome of fish gut T2_DS_IAM_1

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T2_DS_IAM_1


text: IMEDEA

text: public

text: metagenome of fish gut T2_DS_IAM_2

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T2_DS_IAM_2


text: IMEDEA

text: public

text: metagenome of fish gut T2_DS_IAM_3

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T2_DS_IAM_3


text: IMEDEA

text: public

text: metagenome of fish gut T2_DS_IAM_4

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T2_DS_IAM_4


text: IMEDEA

text: public

text: metagenome of fish gut T2_DS_IAM_5

text: Spain

text: fish gut metagenome

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text: fish gut T2_DS_IAM_5


text: IMEDEA

text: public

text: metagenome of fish gut T2_DS_IAM_6

text: Spain

text: fish gut metagenome

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text: fish gut T2_DS_IAM_6


text: IMEDEA

text: public

text: metagenome of fish gut T2_DS_IAM_7

text: Spain

text: fish gut metagenome

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text: fish gut T2_DS_IAM_7


text: IMEDEA

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text: metagenome of fish gut T2_DS_IAM_8

text: Spain

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text: Spain

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text: Spain

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text: public

text: metagenome of fish gut T3_DS_IPM_3

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T3_DS_IPM_3


text: IMEDEA

text: public

text: metagenome of fish gut T3_DS_IPM_4

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T3_DS_IPM_4


text: IMEDEA

text: public

text: metagenome of fish gut T3_DS_IPM_5

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T3_DS_IPM_5


text: IMEDEA

text: public

text: metagenome of fish gut T3_DS_IPM_6

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T3_DS_IPM_6


text: IMEDEA

text: public

text: metagenome of fish gut T3_DS_IPM_7

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T3_DS_IPM_7


text: IMEDEA

text: public

text: metagenome of fish gut T3_DS_IPM_8

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T3_DS_IPM_8


text: IMEDEA

text: public

text: metagenome of comercial_fed_T3_OPT1

text: Spain

text: fish gut metagenome

text: fish gut metagenome


text: IMEDEA

text: public

text: metagenome of comercial_fed_T3_OPT2

text: Spain

text: fish gut metagenome

text: fish gut metagenome


text: IMEDEA

text: public

text: metagenome of comercial_fed_T3_OPT3

text: Spain

text: fish gut metagenome

text: fish gut metagenome


text: IMEDEA

text: public

text: metagenome of fish gut T3_OPT_IAM_1

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T3_OPT_IAM_1


text: IMEDEA

text: public

text: metagenome of fish gut T3_OPT_IAM_2

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T3_OPT_IAM_2


text: IMEDEA

text: public

text: metagenome of fish gut T3_OPT_IAM_3

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T3_OPT_IAM_3


text: IMEDEA

text: public

text: metagenome of fish gut T3_OPT_IAM_4

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T3_OPT_IAM_4


text: IMEDEA

text: public

text: metagenome of fish gut T3_OPT_IAM_5

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T3_OPT_IAM_5


text: IMEDEA

text: public

text: metagenome of fish gut T3_OPT_IAM_6

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T3_OPT_IAM_6


text: IMEDEA

text: public

text: metagenome of fish gut T3_OPT_IAM_7

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T3_OPT_IAM_7


text: IMEDEA

text: public

text: metagenome of fish gut T3_OPT_IAM_8

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T3_OPT_IAM_8


text: IMEDEA

text: public

text: metagenome of fish gut T3_OPT_IPM_1

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T3_OPT_IPM_1


text: IMEDEA

text: public

text: metagenome of fish gut T3_OPT_IPM_2

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T3_OPT_IPM_2


text: IMEDEA

text: public

text: metagenome of fish gut T3_OPT_IPM_3

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T3_OPT_IPM_3


text: IMEDEA

text: public

text: metagenome of fish gut T3_OPT_IPM_4

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T3_OPT_IPM_4


text: IMEDEA

text: public

text: metagenome of fish gut T3_OPT_IPM_5

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T3_OPT_IPM_5


text: IMEDEA

text: public

text: metagenome of fish gut T3_OPT_IPM_6

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T3_OPT_IPM_6


text: IMEDEA

text: public

text: metagenome of fish gut T3_OPT_IPM_7

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T3_OPT_IPM_7


text: IMEDEA

text: public

text: metagenome of fish gut T3_OPT_IPM_8

text: Spain

text: fish gut metagenome

text: fish gut metagenome

text: fish gut T3_OPT_IPM_8


text: HAP1 SGE-MAVE

characteristic: ArrayExpress-PHENOTYPE

text: not applicable

characteristic: ArrayExpress-SEX

text: Homo sapiens

characteristic: ArrayExpress-SPECIES

text: HAP1 lig4- Cas9+ (Sanger 3C Cas9 clone)

text: Not Applicable

characteristic: GENDER

text: SC

text: public

text: HAP1 SGE-MAVE

characteristic: PHENOTYPE

text: human

text: not provided

text: Homo sapiens

text: Homo sapiens


text: QC-PLASMID for HAP1 SGE-MAVE

text: not applicable

text: Homo sapiens

text: Stellar Chemically Competent E.coli

text: Not Applicable

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: QC-PLASMID for HAP1 SGE-MAVE

characteristic: SRA accession

text: human

text: United Kingdom

text: Homo sapiens

text: Homo sapiens


text: University of Helsinki

text: public

tag: Namespace:University of Helsinki

characteristic: Submitter Id

text: University of Helsinki

characteristic: collection date

text: Carbapenemase-producing E.coli in wastewater sample

characteristic: description

text: Finland

characteristic: geographic location (country and/or sea)

text: Escherichia coli

characteristic: organism

text: Escherichia coli


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Helsinki

text: public

text: University of Helsinki

text: Carbapenemase-producing K. pneumoniae in wastewater sample

text: Finland

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: Shanghai Tenth People's Hospital

text: public

tag: Namespace:Shanghai Tenth People's Hospital

text: ArrayExpress

characteristic: broker name

text: not collected

text: Protocols: Cells were grown in YPD broth at 30˚C from OD600=0.01 till OD600=1.0. Pellets of 5ml cell culture were obtained by centrifuging at maximum speed for 1min. the pellets were fast frozen in liquid nitrogen. RNA was extracted using the TRIzol method following the manufacturer's protocol. Cell pellets were transferred from liquid nitrogen into the 2ml tub containing 1.5ml Trizol reagent. The mix was shared for 3min, and kept for 5in at room temperature, then centrifuged at 10,000xg for 5min at 4˚C. The supernatant was added 200µl of chloroform/isoamyl alcohol (24:1) with 1ml lysis reagents. After centrifuged at 10,000g for 10min at 4˚C, the supernatant was transferred into another new tube with equal volume of isopropanol and put in the refrigerator at -20˚C for 1h. After centrifuged at 13600xg for 20min at 4˚C, the supernatant was precipitated by 1ml of 75% ethanol and let dry for 3-5min. The RNA pellet was dissolved with 30-100µl of DEPC water. The concentration of the extracted RNA was determined using a Nanodrop system (NanoDrop, Madison, USA), and the integrity of the RNA was examined by the RNA integrity number (RIN) using an Agilent 2100 bioanalyzer (Agilent, Santa Clara, USA) buffer at appropriate temperature. Then First-strand cDNA was generated using random hexamer-primed reverse transcription, followed by a second-strand cDNA synthesis. afterwards, A-Tailing Mix and RNA Index Adapters were added by incubating to end repair. The cDNA fragments obtained from previous step were amplified by PCR, and products were purified by Ampure XP Beads, then dissolved in EB solution. The product was validated on the Agilent Technologies 2100 bioanalyzer for quality control. The double stranded PCR products from previous step were heated denatured and circularized by the splint oligo sequence to get the final library. The single strand circle DNA (ssCir DNA) was formatted as the final library.

text: wild type genotype

characteristic: genotype

text: not collected

text: not applicable

characteristic: isolate

text: Candida albicans

text: Candida albicans

text: whole organism

characteristic: organism part

text: Candida albicans

characteristic: title


text: Shanghai Tenth People's Hospital

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: not collected

text: trisomy of chromosome R

text: not collected

text: not applicable

text: Candida albicans

text: Candida albicans

text: whole organism

text: Candida albicans


text: Shanghai Tenth People's Hospital

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: not collected

text: segmental monosomy of chromosome 1

text: not collected

text: not applicable

text: Candida albicans

text: Candida albicans

text: whole organism

text: Candida albicans


text: public

text: E-MTAB-13216:3D-HCT-116-Irino-DMSO-Barcode

tag: Namespace:PhD Student  Middle East Technical University

text: ArrayExpress

text: HCT 116

text: colorectal cancer cell

text: not collected

characteristic: collection date

text: human

characteristic: common name

text: Protocols: Cell lines were trypsinized and pelleted after they become resistant. Genomic DNA isolation was performed using GeneJET Genomic DNA isolation kit (ThermoFisher, U.S.A.) according to manufacturer’s recommendations. For barcode sequencing, Library preparation protocol is based on two rounds of PCR to amplify the integrated barcodes from the genomic DNA and carried out according to manufacturer’s guidelines provided by the Cellecta. Ten whole exome sequencing libraries were prepared from genomic DNA by using Twist Comprehensive Exome kit according to the manufacturer’s instructions.

characteristic: description

text: adult

characteristic: developmental stage

text: colorectal adenocarcinoma

characteristic: disease

text: wild type genotype

text: not collected

characteristic: geographic location (country and/or sea)

text: spheroid culture

characteristic: growth condition

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: PhD Student  Middle East Technical University

text: public

text: ArrayExpress

characteristic: broker name

text: HCT 116

text: colorectal cancer cell

text: not collected

text: human

text: adult

text: colorectal adenocarcinoma

text: wild type genotype

characteristic: genotype

text: not collected

text: spheroid culture

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: colon

text: Homo sapiens


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-13216:3D-HCT-116-Irino-Initial-Barcode

text: ArrayExpress

text: HCT 116

text: colorectal cancer cell

text: not collected

text: human

text: adult

text: colorectal adenocarcinoma

text: wild type genotype

text: not collected

text: spheroid culture

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: colon

characteristic: organism part

text: Homo sapiens

characteristic: title


text: public

characteristic: SRA accession

text: E-MTAB-13216:3D-HCT-116-Irino-Initial-WES

characteristic: Submitter Id

text: ArrayExpress

text: HCT 116

text: colorectal cancer cell

text: not collected

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: colorectal adenocarcinoma

characteristic: disease

text: wild type genotype

text: not collected

text: spheroid culture

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-13216:3D-HCT-116-Irino-RepA-Barcode

text: ArrayExpress

characteristic: broker name

text: HCT 116

text: colorectal cancer cell

text: not collected

text: human

text: adult

text: colorectal adenocarcinoma

text: wild type genotype

text: not collected

characteristic: geographic location (country and/or sea)

text: spheroid culture

characteristic: growth condition

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-13216:3D-HCT-116-Irino-RepB-Barcode

text: ArrayExpress

text: HCT 116

text: colorectal cancer cell

text: not collected

text: human

text: adult

text: colorectal adenocarcinoma

text: wild type genotype

characteristic: genotype

text: not collected

text: spheroid culture

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: colon

text: Homo sapiens


text: public

characteristic: SRA accession

text: E-MTAB-13216:3D-HCT-116-Irino-RepC-Barcode

characteristic: Submitter Id

text: ArrayExpress

text: HCT 116

text: colorectal cancer cell

text: not collected

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

text: colorectal adenocarcinoma

text: wild type genotype

text: not collected

text: spheroid culture

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: colon

characteristic: organism part

text: Homo sapiens

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-13216:3D-HT-29-Dabra-DMSO-Barcode

text: ArrayExpress

characteristic: broker name

text: colorectal cancer cell

text: not collected

text: human

text: adult

characteristic: developmental stage

text: colorectal adenocarcinoma

characteristic: disease

text: wild type genotype

text: not collected

text: spheroid culture

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: colorectal cancer cell

text: not collected

text: human

text: adult

text: colorectal adenocarcinoma

text: wild type genotype

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

text: spheroid culture

characteristic: growth condition

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens


text: public

characteristic: SRA accession

text: E-MTAB-13216:3D-HT-29-Dabra-Initial-Barcode

characteristic: Submitter Id

text: ArrayExpress

text: colorectal cancer cell

text: not collected

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

text: colorectal adenocarcinoma

text: wild type genotype

text: not collected

text: spheroid culture

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: colon

text: Homo sapiens


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-13216:3D-HT-29-Dabra-RepA-Barcode

text: ArrayExpress

characteristic: broker name

text: colorectal cancer cell

text: not collected

text: human

text: adult

text: colorectal adenocarcinoma

text: wild type genotype

text: not collected

text: spheroid culture

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: colon

characteristic: organism part

text: Homo sapiens

characteristic: title


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-13216:3D-HT-29-Dabra-RepB-Barcode

text: ArrayExpress

text: colorectal cancer cell

text: not collected

text: human

text: adult

characteristic: developmental stage

text: colorectal adenocarcinoma

characteristic: disease

text: wild type genotype

characteristic: genotype

text: not collected

text: spheroid culture

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens


text: public

characteristic: SRA accession

text: E-MTAB-13216:3D-HT-29-Dabra-RepC-Barcode

characteristic: Submitter Id

text: ArrayExpress

text: colorectal cancer cell

text: not collected

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

text: colorectal adenocarcinoma

text: wild type genotype

text: not collected

characteristic: geographic location (country and/or sea)

text: spheroid culture

characteristic: growth condition

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens


E-MTAB-13226:Sample 1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Quadram Institute Bioscience

text: public

text: E-MTAB-13226:Sample 1

tag: Namespace:Quadram Institute Bioscience

text: ArrayExpress

characteristic: broker name

text: not collected

text: Protocols: The E. coli BW25113 transposon mutant library used in this study contains over 800,000 distinct mutants and has recently been described by Yasir et al. (2020). Approximately 107 CFU/mL of this library was added to 1 mL LB broth supplemented with concentrations of piperacillin around the MIC (MIC was 4 µg/mL piperacillin, concentrations used were ¼x, ½x, 1x and 2x MIC). Tazobactam was either added or omitted from these conditions at a concentration of 32 µg/mL. The Tn5 transposon used to create this library contains an outward-transcribing tac promoter, which was either induced with 0.2 or 1 µM IPTG or left uninduced. Each condition was carried out in duplicate with two unstressed controls containing no drug. Cultures were grown for 24 hours at 37 ⁰C shaking and were centrifuged at 3000 x g for 10 minutes to pellet the cells DNA was extracted from cell pellets following the protocol described by Trampari et al. (2021) and quantified using a Qubit HS assay kit (Invitrogen). The library was fragmented with the MuSeek DNA fragment library preparation kit (ThermoFisher) and purified using AMPure XP beads (Beckman Coulter). Fragments containing the transposon were amplified by PCR using customised biotinylated sequencing primers specific for the transposon tag and the PCR product was again purified as previously described. To extract biotinylated fragments containing the Tn5 transposon sequence, samples were incubated for 4 hours with activated beads from the Dynabeads® kilobaseBINDER™ kit (Invitrogen). Following this, Dynabeads® were resuspended in elution buffer and used as the template for a second PCR amplification step, using customised primers specific for the transposon and those specific for the MuSeek tag. Dynabeads® were removed from the PCR product using a magnetic stand. The resulting sequencing library was purified twice and fragments were selected between 300-500 bp in length.

text: not collected

text: not applicable

characteristic: isolate

text: Escherichia coli

characteristic: organism

text: Escherichia coli

text: whole organism

text: no induction

characteristic: phenotype

characteristic: replicate

text: Escherichia coli

text: no IPTG

characteristic: stimulus

characteristic: strain

text: Sample 1


E-MTAB-13226:Sample 10

text: Quadram Institute Bioscience

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13226:Sample 10

characteristic: Submitter Id

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

characteristic: organism part

text: IPTG-induced expression of Tn5 transposon

text: Escherichia coli

text: 0.2 µM IPTG

text: Sample 10

characteristic: title


E-MTAB-13226:Sample 11

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Quadram Institute Bioscience

text: public

text: E-MTAB-13226:Sample 11

text: ArrayExpress

text: not collected

characteristic: collection date

characteristic: description

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

text: Escherichia coli

text: 1 µM IPTG

text: Sample 11


E-MTAB-13226:Sample 12

characteristic: External Id

text: Quadram Institute Bioscience

text: public

text: E-MTAB-13226:Sample 12

text: ArrayExpress

characteristic: broker name

text: not collected

text: not collected

text: not applicable

characteristic: isolate

text: Escherichia coli

characteristic: organism

text: Escherichia coli

text: whole organism

characteristic: phenotype

characteristic: replicate

text: Escherichia coli

text: 1 µM IPTG

text: Sample 12


E-MTAB-13226:Sample 13

text: Quadram Institute Bioscience

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-13226:Sample 13

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

text: no induction

text: Escherichia coli

text: no IPTG

characteristic: stimulus

characteristic: strain

text: Sample 13


E-MTAB-13226:Sample 14

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Quadram Institute Bioscience

text: public

characteristic: SRA accession

text: E-MTAB-13226:Sample 14

characteristic: Submitter Id

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

characteristic: organism part

text: no induction

text: Escherichia coli

text: no IPTG

text: Sample 14

characteristic: title


E-MTAB-13226:Sample 15

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Quadram Institute Bioscience

text: public

text: E-MTAB-13226:Sample 15

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

characteristic: description

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

text: Escherichia coli

text: 0.2 µM IPTG

text: Sample 15


E-MTAB-13226:Sample 16

text: Quadram Institute Bioscience

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-13226:Sample 16

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

characteristic: isolate

text: Escherichia coli

characteristic: organism

text: Escherichia coli

text: whole organism

characteristic: phenotype

characteristic: replicate

text: Escherichia coli

text: 0.2 µM IPTG

text: Sample 16


E-MTAB-13226:Sample 17

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Quadram Institute Bioscience

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-13226:Sample 17

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

text: Escherichia coli

text: 1 µM IPTG

characteristic: stimulus

characteristic: strain

text: Sample 17


E-MTAB-13226:Sample 18

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Quadram Institute Bioscience

text: public

characteristic: SRA accession

text: E-MTAB-13226:Sample 18

characteristic: Submitter Id

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

characteristic: organism part

text: Escherichia coli

text: 1 µM IPTG

text: Sample 18

characteristic: title


E-MTAB-13226:Sample 19

text: Quadram Institute Bioscience

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-13226:Sample 19

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

characteristic: description

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

text: no induction

text: Escherichia coli

text: no IPTG

text: Sample 19


E-MTAB-13226:Sample 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Quadram Institute Bioscience

text: public

text: E-MTAB-13226:Sample 2

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

characteristic: isolate

text: Escherichia coli

characteristic: organism

text: Escherichia coli

text: whole organism

text: no induction

characteristic: phenotype

characteristic: replicate

text: Escherichia coli

text: no IPTG

text: Sample 2


E-MTAB-13226:Sample 20

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Quadram Institute Bioscience

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-13226:Sample 20

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

text: no induction

text: Escherichia coli

text: no IPTG

characteristic: stimulus

characteristic: strain

text: Sample 20


E-MTAB-13226:Sample 21

text: Quadram Institute Bioscience

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-13226:Sample 21

characteristic: Submitter Id

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

characteristic: organism part

text: Escherichia coli

text: 0.2 µM IPTG

text: Sample 21

characteristic: title


E-MTAB-13226:Sample 22

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Quadram Institute Bioscience

text: public

text: E-MTAB-13226:Sample 22

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

characteristic: description

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

text: Escherichia coli

text: 0.2 µM IPTG

text: Sample 22


E-MTAB-13226:Sample 23

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Quadram Institute Bioscience

text: public

text: E-MTAB-13226:Sample 23

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

characteristic: isolate

text: Escherichia coli

characteristic: organism

text: Escherichia coli

text: whole organism

characteristic: phenotype

characteristic: replicate

text: Escherichia coli

text: 1 µM IPTG

text: Sample 23


E-MTAB-13226:Sample 24

text: Quadram Institute Bioscience

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-13226:Sample 24

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

text: Escherichia coli

text: 1 µM IPTG

characteristic: stimulus

characteristic: strain

text: Sample 24


E-MTAB-13226:Sample 25

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Quadram Institute Bioscience

text: public

characteristic: SRA accession

text: E-MTAB-13226:Sample 25

characteristic: Submitter Id

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

characteristic: organism part

text: no induction

text: Escherichia coli

text: no IPTG

text: Sample 25

characteristic: title


E-MTAB-13226:Sample 26

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Quadram Institute Bioscience

text: public

text: E-MTAB-13226:Sample 26

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

characteristic: description

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

text: no induction

text: Escherichia coli

text: no IPTG

text: Sample 26


E-MTAB-13226:Sample 27

text: Quadram Institute Bioscience

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-13226:Sample 27

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

characteristic: isolate

text: Escherichia coli

characteristic: organism

text: Escherichia coli

text: whole organism

characteristic: phenotype

characteristic: replicate

text: Escherichia coli

text: 0.2 µM IPTG

text: Sample 27


E-MTAB-13226:Sample 28

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Quadram Institute Bioscience

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-13226:Sample 28

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

text: Escherichia coli

text: 0.2 µM IPTG

characteristic: stimulus

characteristic: strain

text: Sample 28


E-MTAB-13226:Sample 29

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Quadram Institute Bioscience

text: public

characteristic: SRA accession

text: E-MTAB-13226:Sample 29

characteristic: Submitter Id

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

characteristic: organism part

text: Escherichia coli

text: 1 µM IPTG

text: Sample 29

characteristic: title


E-MTAB-13226:Sample 3

text: Quadram Institute Bioscience

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-13226:Sample 3

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

characteristic: description

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

text: Escherichia coli

text: 0.2 µM IPTG

text: Sample 3


E-MTAB-13226:Sample 30

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Quadram Institute Bioscience

text: public

text: E-MTAB-13226:Sample 30

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

characteristic: isolate

text: Escherichia coli

characteristic: organism

text: Escherichia coli

text: whole organism

characteristic: phenotype

characteristic: replicate

text: Escherichia coli

text: 1 µM IPTG

text: Sample 30


E-MTAB-13226:Sample 31

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Quadram Institute Bioscience

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-13226:Sample 31

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

text: no induction

text: Escherichia coli

text: no IPTG

characteristic: stimulus

characteristic: strain

text: Sample 31


E-MTAB-13226:Sample 32

text: Quadram Institute Bioscience

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-13226:Sample 32

characteristic: Submitter Id

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

characteristic: organism part

text: no induction

text: Escherichia coli

text: no IPTG

text: Sample 32

characteristic: title


E-MTAB-13226:Sample 33

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Quadram Institute Bioscience

text: public

text: E-MTAB-13226:Sample 33

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

characteristic: description

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

text: Escherichia coli

text: 0.2 µM IPTG

text: Sample 33


E-MTAB-13226:Sample 34

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Quadram Institute Bioscience

text: public

text: E-MTAB-13226:Sample 34

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

characteristic: isolate

text: Escherichia coli

characteristic: organism

text: Escherichia coli

text: whole organism

characteristic: phenotype

characteristic: replicate

text: Escherichia coli

text: 0.2 µM IPTG

text: Sample 34


E-MTAB-13226:Sample 35

text: Quadram Institute Bioscience

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-13226:Sample 35

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

text: Escherichia coli

text: 1 µM IPTG

characteristic: stimulus

characteristic: strain

text: Sample 35


E-MTAB-13226:Sample 36

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Quadram Institute Bioscience

text: public

characteristic: SRA accession

text: E-MTAB-13226:Sample 36

characteristic: Submitter Id

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

characteristic: organism part

text: Escherichia coli

text: 1 µM IPTG

text: Sample 36

characteristic: title


E-MTAB-13226:Sample 37

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Quadram Institute Bioscience

text: public

text: E-MTAB-13226:Sample 37

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

characteristic: description

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

text: no induction

text: Escherichia coli

text: no IPTG

text: Sample 37


E-MTAB-13226:Sample 38

text: Quadram Institute Bioscience

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-13226:Sample 38

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

characteristic: isolate

text: Escherichia coli

characteristic: organism

text: Escherichia coli

text: whole organism

text: no induction

characteristic: phenotype

characteristic: replicate

text: Escherichia coli

text: no IPTG

text: Sample 38


E-MTAB-13226:Sample 39

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Quadram Institute Bioscience

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-13226:Sample 39

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

text: Escherichia coli

text: 0.2 µM IPTG

characteristic: stimulus

characteristic: strain

text: Sample 39


E-MTAB-13226:Sample 4

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Quadram Institute Bioscience

text: public

characteristic: SRA accession

text: E-MTAB-13226:Sample 4

characteristic: Submitter Id

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

characteristic: organism part

text: Escherichia coli

text: 0.2 µM IPTG

text: Sample 4

characteristic: title


E-MTAB-13226:Sample 40

text: Quadram Institute Bioscience

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-13226:Sample 40

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

characteristic: description

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

text: Escherichia coli

text: 0.2 µM IPTG

text: Sample 40


E-MTAB-13226:Sample 41

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Quadram Institute Bioscience

text: public

text: E-MTAB-13226:Sample 41

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

characteristic: isolate

text: Escherichia coli

characteristic: organism

text: Escherichia coli

text: whole organism

characteristic: phenotype

characteristic: replicate

text: Escherichia coli

text: 1 µM IPTG

text: Sample 41


E-MTAB-13226:Sample 42

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Quadram Institute Bioscience

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-13226:Sample 42

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

text: Escherichia coli

text: 1 µM IPTG

characteristic: stimulus

characteristic: strain

text: Sample 42


E-MTAB-13226:Sample 43

text: Quadram Institute Bioscience

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-13226:Sample 43

characteristic: Submitter Id

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

characteristic: organism part

text: no induction

text: Escherichia coli

text: no IPTG

text: Sample 43

characteristic: title


E-MTAB-13226:Sample 44

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Quadram Institute Bioscience

text: public

text: E-MTAB-13226:Sample 44

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

characteristic: description

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

text: no induction

text: Escherichia coli

text: no IPTG

text: Sample 44


E-MTAB-13226:Sample 45

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Quadram Institute Bioscience

text: public

text: E-MTAB-13226:Sample 45

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

characteristic: isolate

text: Escherichia coli

characteristic: organism

text: Escherichia coli

text: whole organism

characteristic: phenotype

characteristic: replicate

text: Escherichia coli

text: 0.2 µM IPTG

text: Sample 45


E-MTAB-13226:Sample 46

text: Quadram Institute Bioscience

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-13226:Sample 46

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

text: Escherichia coli

text: 0.2 µM IPTG

characteristic: stimulus

characteristic: strain

text: Sample 46


E-MTAB-13226:Sample 47

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Quadram Institute Bioscience

text: public

characteristic: SRA accession

text: E-MTAB-13226:Sample 47

characteristic: Submitter Id

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

characteristic: organism part

text: Escherichia coli

text: 1 µM IPTG

text: Sample 47

characteristic: title


E-MTAB-13226:Sample 48

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Quadram Institute Bioscience

text: public

text: E-MTAB-13226:Sample 48

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

characteristic: description

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

text: Escherichia coli

text: 1 µM IPTG

text: Sample 48


E-MTAB-13226:Sample 49

text: Quadram Institute Bioscience

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-13226:Sample 49

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

characteristic: isolate

text: Escherichia coli

characteristic: organism

text: Escherichia coli

text: whole organism

text: no induction

characteristic: phenotype

characteristic: replicate

text: Escherichia coli

text: no IPTG

text: Sample 49


E-MTAB-13226:Sample 5

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Quadram Institute Bioscience

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-13226:Sample 5

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

text: Escherichia coli

text: 1 µM IPTG

characteristic: stimulus

characteristic: strain

text: Sample 5


E-MTAB-13226:Sample 50

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Quadram Institute Bioscience

text: public

characteristic: SRA accession

text: E-MTAB-13226:Sample 50

characteristic: Submitter Id

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

characteristic: organism part

text: no induction

text: Escherichia coli

text: no IPTG

text: Sample 50

characteristic: title


E-MTAB-13226:Sample 51

text: Quadram Institute Bioscience

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-13226:Sample 51

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

characteristic: description

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

text: Escherichia coli

text: 0.2 µM IPTG

text: Sample 51


E-MTAB-13226:Sample 52

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Quadram Institute Bioscience

text: public

text: E-MTAB-13226:Sample 52

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

characteristic: isolate

text: Escherichia coli

characteristic: organism

text: Escherichia coli

text: whole organism

characteristic: phenotype

characteristic: replicate

text: Escherichia coli

text: 0.2 µM IPTG

text: Sample 52


E-MTAB-13226:Sample 53

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Quadram Institute Bioscience

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-13226:Sample 53

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

text: Escherichia coli

text: 1 µM IPTG

characteristic: stimulus

characteristic: strain

text: Sample 53


E-MTAB-13226:Sample 54

text: Quadram Institute Bioscience

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-13226:Sample 54

characteristic: Submitter Id

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

characteristic: organism part

text: Escherichia coli

text: 1 µM IPTG

text: Sample 54

characteristic: title


E-MTAB-13226:Sample 55

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Quadram Institute Bioscience

text: public

text: E-MTAB-13226:Sample 55

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

characteristic: description

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

text: no induction

text: Escherichia coli

text: no IPTG

text: Sample 55


E-MTAB-13226:Sample 56

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Quadram Institute Bioscience

text: public

text: E-MTAB-13226:Sample 56

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

characteristic: isolate

text: Escherichia coli

characteristic: organism

text: Escherichia coli

text: whole organism

text: no induction

characteristic: phenotype

characteristic: replicate

text: Escherichia coli

text: no IPTG

text: Sample 56


E-MTAB-13226:Sample 57

text: Quadram Institute Bioscience

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-13226:Sample 57

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

text: Escherichia coli

text: 0.2 µM IPTG

characteristic: stimulus

characteristic: strain

text: Sample 57


E-MTAB-13226:Sample 58

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Quadram Institute Bioscience

text: public

characteristic: SRA accession

text: E-MTAB-13226:Sample 58

characteristic: Submitter Id

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

characteristic: organism part

text: Escherichia coli

text: 0.2 µM IPTG

text: Sample 58

characteristic: title


E-MTAB-13226:Sample 59

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Quadram Institute Bioscience

text: public

text: E-MTAB-13226:Sample 59

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

characteristic: description

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

text: Escherichia coli

text: 1 µM IPTG

text: Sample 59


E-MTAB-13226:Sample 6

text: Quadram Institute Bioscience

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-13226:Sample 6

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

characteristic: isolate

text: Escherichia coli

characteristic: organism

text: Escherichia coli

text: whole organism

characteristic: phenotype

characteristic: replicate

text: Escherichia coli

text: 1 µM IPTG

text: Sample 6


E-MTAB-13226:Sample 60

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Quadram Institute Bioscience

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-13226:Sample 60

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

text: Escherichia coli

text: 1 µM IPTG

characteristic: stimulus

characteristic: strain

text: Sample 60


E-MTAB-13226:Sample 7

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Quadram Institute Bioscience

text: public

characteristic: SRA accession

text: E-MTAB-13226:Sample 7

characteristic: Submitter Id

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

characteristic: organism part

text: no induction

text: Escherichia coli

text: no IPTG

text: Sample 7

characteristic: title


E-MTAB-13226:Sample 8

text: Quadram Institute Bioscience

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-13226:Sample 8

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

characteristic: description

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

text: Escherichia coli

text: Escherichia coli

text: whole organism

text: no induction

text: Escherichia coli

text: no IPTG

text: Sample 8


E-MTAB-13226:Sample 9

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Quadram Institute Bioscience

text: public

text: E-MTAB-13226:Sample 9

text: ArrayExpress

text: not collected

text: not collected

text: not applicable

characteristic: isolate

text: Escherichia coli

characteristic: organism

text: Escherichia coli

text: whole organism

characteristic: phenotype

characteristic: replicate

text: Escherichia coli

text: 0.2 µM IPTG

text: Sample 9


text: Mammary gland

characteristic: ArrayExpress-PHENOTYPE

text: female

characteristic: ArrayExpress-SEX

text: Canis familiaris

characteristic: ArrayExpress-SPECIES

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Female

characteristic: GENDER

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Mammary gland

characteristic: PHENOTYPE

text: dog

characteristic: common name

text: Canis lupus familiaris

text: Canis lupus familiaris


text: Skin

text: female

text: Canis familiaris

text: Female

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Skin

characteristic: SRA accession

characteristic: Submitter Id

text: dog

text: Canis lupus familiaris

text: Canis lupus familiaris

characteristic: title


text: Lymph node

text: female

text: Canis familiaris

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Female

text: SC

text: public

text: Lymph node

text: dog

text: Canis lupus familiaris

characteristic: organism

text: Canis lupus familiaris


text: Concordia University

text: public

tag: Namespace:Concordia University

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: Spades;3.12.0;kmer set 33, 55, 77, 99,127, default parameters otherwise

characteristic: assembly software

text: Default parameters; minimum scaffold length 2500 bp

characteristic: binning parameters

text: Metabat2 v.2.12.1

characteristic: binning software

text: marine biome (ENVO:00000447)

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: completeness score

text: CheckM v.1.0.7

characteristic: completeness software

characteristic: contamination score

text: Uncultivated Acidimicrobiia bacterium UBA9410 recovered from ERS15586164

characteristic: description

text: sea water (ENVO:00002149 )

characteristic: environmental medium

text: Arctic Ocean

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: MAG reconstructed from a metagenome collected in the Canada Basin (Arctic Ocean) at depth of 1000m

text: 1000m depth (ENVO:XXXXXXXX)

characteristic: local environmental context

text: aquatic metagenome

characteristic: metagenomic source

text: Acidimicrobiia bacterium

text: Beaufort Gyre Obsering System and Joint Ocean and Ice Studies

characteristic: project name

characteristic: sample derived from

text: Acidimicrobiia bacterium

text: Multi-marker approach

characteristic: taxonomic identity marker

text: Canada Basin MAG CB11b_AW.101


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Concordia University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

characteristic: binning parameters

text: Metabat2 v.2.12.1

text: marine biome (ENVO:00000447)

text: CheckM v.1.0.7

text: Uncultivated bacterium recovered from ERS15586164

text: sea water (ENVO:00002149 )

text: Arctic Ocean

text: 1000m depth (ENVO:XXXXXXXX)

text: aquatic metagenome

text: Nitrospinota bacterium

characteristic: organism

text: Nitrospinota bacterium

text: Multi-marker approach

text: Canada Basin MAG CB11b_AW.107

characteristic: title