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text: Klebsiella pneumoniae


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text: UC

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text: Klebsiella pneumoniae


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text: CGE

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text: DTU2020-159-PRJ1228-Klebsiella-pneumoniae-659

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text: Homo sapiens

text: Klebsiella pneumoniae

text: UC

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


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text: CGE

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text: public

text: DTU2020-170-PRJ1228-Klebsiella-pneumoniae-77

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text: Iran

text: Homo sapiens

text: Klebsiella pneumoniae

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text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


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text: DTU2020-179-PRJ1228-Klebsiella-pneumoniae-351A

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text: Homo sapiens

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text: sputum

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: CGE

text: public

text: DTU2020-180-PRJ1228-Klebsiella-pneumoniae-247

characteristic: Submitter Id

text: DTU-GE

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text: Omid Pajand

text: Iran

text: Homo sapiens

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text: UC

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: CGE

text: public

text: DTU2020-174-PRJ1228-Enterobacter-cloace-191b

text: DTU-GE

text: Omid Pajand

characteristic: description

text: Iran

characteristic: geographic location (country and/or sea)

text: Homo sapiens

text: Enterobacter cloacae

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text: Enterobacter cloacae

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text: DTU2020-167-PRJ1228-Klebsiella-pneumoniae-700

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text: Omid Pajand

text: Iran

text: Homo sapiens

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text: Klebsiella pneumoniae

text: UC

text: Klebsiella pneumoniae

characteristic: organism

text: Klebsiella pneumoniae


text: CGE

text: public

text: DTU2020-138-PRJ1228-Klebsiella-pneumoniae-416_S48

text: DTU-GE

text: Omid Pajand

text: Iran

text: Homo sapiens

text: Klebsiella pneumoniae

text: UC

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

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characteristic: title


text: CGE

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text: public

text: DTU2020-160-PRJ1228-Klebsiella-pneumoniae-670B

text: DTU-GE

text: Omid Pajand

text: Iran

text: Homo sapiens

text: Klebsiella pneumoniae

text: UC

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


characteristic: ENA-CHECKLIST

text: CGE

text: public

text: DTU2020-161-PRJ1228-Klebsiella-pneumoniae-671B

text: DTU-GE

text: Omid Pajand

text: Iran

text: Homo sapiens

text: Klebsiella pneumoniae

characteristic: isolate

text: UC

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


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text: CGE

text: public

text: DTU2020-117-PRJ1228-Klebsiella-pneumoniae-114_S27

text: DTU-GE

text: Omid Pajand

text: Iran

text: Homo sapiens

text: Klebsiella pneumoniae

text: UC

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


characteristic: External Id

text: CGE

characteristic: INSDC last update

text: public

text: DTU2020-172-PRJ1228-Klebsiella-pneumoniae-542

text: DTU-GE

text: Omid Pajand

text: Iran

text: Homo sapiens

text: Klebsiella pneumoniae

text: UC

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: CGE

text: public

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text: DTU2020-125-PRJ1228-Klebsiella-pneumoniae-22_S35

text: DTU-GE

text: Omid Pajand

text: Iran

text: Homo sapiens

text: Klebsiella pneumoniae

text: UC

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Unknown


text: Patrick Favre

text: public

tag: Namespace:Patrick Favre

characteristic: Submitter Id

text: Valentin Gfeller, Tala Bürki

characteristic: collection date

text: sample from collection on the field (i.e. natural population)

unit: m

characteristic: elevation

text: Austria

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: isolation and growth condition

text: Biscutella laevigata

text: Buckler mustard

characteristic: organism common name

text: vegetative shoot system (PO:0025607)

characteristic: plant developmental stage

text: field

characteristic: plant growth medium

text: leaf (PO:0025034)

characteristic: plant structure

text: diploid

characteristic: ploidy

text: Biscutella laevigata

text: subspecies kernerii

characteristic: subspecific genetic lineage rank


text: Plasmodium falciparum

characteristic: ArrayExpress-SPECIES

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

characteristic: INSDC center name

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text: public

text: malaria parasite P. falciparum

characteristic: common name

text: Plasmodium falciparum

characteristic: organism

text: Plasmodium falciparum

characteristic: title


characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:UNIVERSITY OF SZEGED

characteristic: Submitter Id

text: KSHV-positive primary effusion lymphoma cell line

text: primary lymphoma

text: Kaposi's sarcoma-associated herpesvirus mixed infection CAGE

characteristic: description

text: USA

characteristic: geographic location (country and/or sea)

text: Homo sapiens

characteristic: host scientific name

text: human cell line

text: Human gammaherpesvirus 8

text: Human gammaherpesvirus 8

text: bone marrow


Frankia Hr75

text: public

text: Frankia Hr75

text: Frankia Hr75

text: Frankia sp. Hr75.2

text: Frankia sp. Hr75.2

text: Frankia Hr75


text: public

tag: Namespace:Centre for Interdisciplinary Research in Animal Health (CIISA), Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal

text: Portugal

text: diseased

characteristic: host health state

text: Canis lupus familiaris

characteristic: isolate

text: Skin swab

text: Escherichia coli

text: Escherichia coli

characteristic: serovar


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Centre for Interdisciplinary Research in Animal Health (CIISA), Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal

text: public

text: Portugal

text: diseased

text: Canis lupus familiaris

text: Urine

text: Escherichia coli

text: Escherichia coli


characteristic: INSDC center name

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text: public

text: Portugal

text: diseased

text: Canis lupus familiaris

text: Faeces

text: Escherichia coli

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text: public

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text: diseased

text: Canis lupus familiaris

text: Urine

text: Klebsiella pneumoniae

characteristic: organism

text: Klebsiella pneumoniae

characteristic: title


characteristic: External Id

characteristic: INSDC last update

text: public

text: United Kingdom

text: healthy

text: homo sapiens sapiens

text: Faeces

text: Escherichia coli

text: Escherichia coli


text: public

characteristic: INSDC status

characteristic: SRA accession

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text: Canis lupus familiaris

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text: Escherichia coli

text: Escherichia coli


text: public

characteristic: Submitter Id

text: United Kingdom

text: diseased

text: Canis lupus familiaris

text: Nasal swab

text: Escherichia coli

text: Escherichia coli


text: public

text: Portugal

characteristic: geographic location (country and/or sea)

text: healthy

text: homo sapiens sapiens

text: Faeces

text: Escherichia coli

text: Escherichia coli


text: Technical University of Denmark

text: public

tag: Namespace:Technical University of Denmark

text: Human oral cavity

characteristic: broad-scale environmental context

characteristic: collection date

text: human

characteristic: common name

text: Saliva sample

characteristic: description

text: Saliva

characteristic: environmental medium

text: Sweden

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Whole saliva

characteristic: local environmental context

text: Homo sapiens

text: Oral microbiome

characteristic: project name

text: Homo sapiens


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Technical University of Denmark

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Technical control

text: human

text: Technical control

text: Technical control

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text: Technical control

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text: Technical control

text: Homo sapiens


characteristic: External Id

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:INRES Molecular Biology of the Rhizosphere

characteristic: Submitter Id

text: alpine tundra biome

characteristic: depth

characteristic: elevation

text: silty soil

text: Germany

text: pine rhizosphere

text: uncultured prokaryote

text: uncultured prokaryote

text: 8303 DNA

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text: INRES Molecular Biology of the Rhizosphere

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text: alpine tundra biome

characteristic: broad-scale environmental context

characteristic: collection date

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text: silty soil

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text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: pine rhizosphere

text: uncultured prokaryote

text: uncultured prokaryote

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characteristic: ENA-CHECKLIST

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text: public

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characteristic: External Id

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text: alpine tundra biome

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text: silty soil

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text: beech rhizosphere

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characteristic: organism

text: uncultured prokaryote

text: 8306 DNA


text: public

text: alpine tundra biome

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: description

characteristic: elevation

text: silty soil

characteristic: environmental medium

text: Germany

characteristic: geographic location (country and/or sea)

text: beech rhizosphere

text: uncultured prokaryote

text: uncultured prokaryote

text: 8307 DNA

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: alpine tundra biome

text: silty soil

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: beech rhizosphere

text: uncultured prokaryote

text: uncultured prokaryote

text: 8308 DNA


characteristic: External Id

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: alpine tundra biome

text: silty soil

text: Germany

text: bulk soil

characteristic: local environmental context

text: uncultured prokaryote

characteristic: project name

text: uncultured prokaryote

text: 8309 DNA


text: public

text: alpine tundra biome

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: elevation

text: sandy soil

characteristic: environmental medium

text: Germany

text: beech rhizosphere

text: uncultured prokaryote

characteristic: organism

text: uncultured prokaryote

text: 8310 DNA


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: alpine tundra biome

text: sand org_fagus

text: sandy soil org

text: Germany

characteristic: geographic location (country and/or sea)

text: beech rhizosphere

text: uncultured prokaryote

text: uncultured prokaryote

text: 8311 DNA

characteristic: title


characteristic: External Id

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: forest biome

text: silty soil

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: bulk soil

text: uncultured prokaryote

text: uncultured prokaryote

text: 8312 DNA


text: public

text: forest biome

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

text: sand org_fagus

characteristic: description

characteristic: elevation

text: sandy soil org

text: Germany

text: beech rhizosphere

characteristic: local environmental context

text: uncultured prokaryote

characteristic: project name

text: uncultured prokaryote

text: 8314 DNA


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: forest biome

text: sand org_fagus

text: sandy soil org

characteristic: environmental medium

text: Germany

text: beech rhizosphere

text: uncultured prokaryote

characteristic: organism

text: uncultured prokaryote

text: 8315 DNA


characteristic: External Id

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: forest biome

text: sand mineral_fagus

text: sandy soil

text: Germany

characteristic: geographic location (country and/or sea)

text: beech rhizosphere

text: uncultured prokaryote

text: uncultured prokaryote

text: 8316 DNA

characteristic: title


text: public

text: forest biome

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

text: sand mineral_pine

characteristic: description

characteristic: elevation

text: sandy soil

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: pine rhizosphere

text: uncultured prokaryote

text: uncultured prokaryote

text: 8318 DNA


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: forest biome

text: sandy soil

text: Germany

text: bulk soil

characteristic: local environmental context

text: uncultured prokaryote

characteristic: project name

text: uncultured prokaryote

text: 8326 DNA


characteristic: External Id

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: forest biome

text: sandy soil

characteristic: environmental medium

text: Germany

text: bulk soil

text: uncultured prokaryote

characteristic: organism

text: uncultured prokaryote

text: 8327 DNA


text: public

text: forest biome

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: elevation

text: sandy soil

text: Germany

characteristic: geographic location (country and/or sea)

text: bulk soil

text: uncultured prokaryote

text: uncultured prokaryote

text: 8330 DNA

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: forest biome

text: negativ

text: negativ

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: negativ

text: uncultured prokaryote

text: uncultured prokaryote

text: negativ


characteristic: External Id

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: forest biome

text: silty soil

text: Germany

text: pine rhizosphere

characteristic: local environmental context

text: uncultured prokaryote

characteristic: project name

text: uncultured prokaryote

text: 8303 cDNA


text: public

text: forest biome

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: elevation

text: silty soil

characteristic: environmental medium

text: Germany

text: pine rhizosphere

text: uncultured prokaryote

characteristic: organism

text: uncultured prokaryote

text: 8304 cDNA


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: forest biome

text: silty soil

text: Germany

characteristic: geographic location (country and/or sea)

text: pine rhizosphere

text: uncultured prokaryote

text: uncultured prokaryote

text: 8305 cDNA

characteristic: title


characteristic: External Id

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: forest biome

text: silty soil

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: beech rhizosphere

text: uncultured prokaryote

text: uncultured prokaryote

text: 8306 cDNA


text: public

text: forest biome

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: elevation

text: silty soil

text: Germany

text: beech rhizosphere

characteristic: local environmental context

text: uncultured prokaryote

characteristic: project name

text: uncultured prokaryote

text: 8307 cDNA


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: forest biome

text: silty soil

characteristic: environmental medium

text: Germany

text: beech rhizosphere

text: uncultured prokaryote

characteristic: organism

text: uncultured prokaryote

text: 8308 cDNA


characteristic: External Id

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: forest biome

text: silty soil

text: Germany

characteristic: geographic location (country and/or sea)

text: bulk soil

text: uncultured prokaryote

text: uncultured prokaryote

text: 8309 cDNA

characteristic: title


text: public

text: forest biome

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: elevation

text: sandy soil

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: beech rhizosphere

text: uncultured prokaryote

text: uncultured prokaryote

text: 8310 cDNA


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: forest biome

text: sand org_fagus

text: sandy soil org

text: Germany

text: beech rhizosphere

characteristic: local environmental context

text: uncultured prokaryote

characteristic: project name

text: uncultured prokaryote

text: 8311 cDNA


characteristic: External Id

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: forest biome

text: silty soil

characteristic: environmental medium

text: Germany

text: bulk soil

text: uncultured prokaryote

characteristic: organism

text: uncultured prokaryote

text: 8312 cDNA


text: public

text: forest biome

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

text: sand org_fagus

characteristic: description

characteristic: elevation

text: sandy soil org

text: Germany

characteristic: geographic location (country and/or sea)

text: beech rhizosphere

text: uncultured prokaryote

text: uncultured prokaryote

text: 8314 cDNA

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: forest biome

text: sand org_fagus

text: sandy soil org

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: beech rhizosphere

text: uncultured prokaryote

text: uncultured prokaryote

text: 8315 cDNA


characteristic: External Id

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: forest biome

text: sand mineral_fagus

text: sandy soil

text: Germany

text: beech rhizosphere

characteristic: local environmental context

text: uncultured prokaryote

characteristic: project name

text: uncultured prokaryote

text: 8316 cDNA


text: public

text: forest biome

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

text: sand mineral_fagus

characteristic: description

characteristic: elevation

text: sandy soil

characteristic: environmental medium

text: Germany

text: beech rhizosphere

text: uncultured prokaryote

characteristic: organism

text: uncultured prokaryote

text: 8317 cDNA


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: forest biome

text: sand mineral_pine

text: sandy soil

text: Germany

characteristic: geographic location (country and/or sea)

text: pine rhizosphere

text: uncultured prokaryote

text: uncultured prokaryote

text: 8318 cDNA

characteristic: title


characteristic: External Id

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: forest biome

text: sand mineral_pine

text: sandy soil

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: pine rhizosphere

text: uncultured prokaryote

text: uncultured prokaryote

text: 8319 cDNA


text: public

text: forest biome

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: elevation

text: sandy soil

text: Germany

text: bulk soil

characteristic: local environmental context

text: uncultured prokaryote

characteristic: project name

text: uncultured prokaryote

text: 8325 cDNA


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: forest biome

text: sandy soil

characteristic: environmental medium

text: Germany

text: bulk soil

text: uncultured prokaryote

characteristic: organism

text: uncultured prokaryote

text: 8326 cDNA


characteristic: External Id

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: forest biome

text: sandy soil

text: Germany

characteristic: geographic location (country and/or sea)

text: bulk soil

text: uncultured prokaryote

text: uncultured prokaryote

text: 8327 cDNA

characteristic: title


text: public

text: forest biome

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: elevation

text: sandy soil

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: bulk soil

text: uncultured prokaryote

text: uncultured prokaryote

text: 8330 cDNA


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: forest biome

text: negativ

text: negativ

text: Germany

text: negativ

characteristic: local environmental context

text: uncultured prokaryote

characteristic: project name

text: uncultured prokaryote

text: negativ


characteristic: External Id

text: SOUTH CHINA NORMAL UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:SOUTH CHINA NORMAL UNIVERSITY

characteristic: Submitter Id

text: uncultured bacterium

characteristic: organism

text: uncultured bacterium

text: Five soil samples.


text: public

text: tree and shrub savanna

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

text: cropland

characteristic: environmental medium

text: Namibia

characteristic: geographic location (country and/or sea)

text: maize

text: uncultured prokaryote

text: uncultured prokaryote

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: tree and shrub savanna

characteristic: elevation

text: low elefant density

text: Namibia

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: tree

text: uncultured prokaryote

text: uncultured prokaryote


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: tree and shrub savanna

text: high elow elefant densityefant density

text: Namibia

text: gras

characteristic: local environmental context

text: uncultured prokaryote

characteristic: project name

text: uncultured prokaryote


text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: tree and shrub savanna

characteristic: broad-scale environmental context

characteristic: collection date

text: high elow elefant densityefant density

text: Namibia

text: tree

text: uncultured prokaryote

characteristic: organism

text: uncultured prokaryote


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: tree and shrub savanna

characteristic: depth

text: rangeland

characteristic: environmental medium

text: Namibia

characteristic: geographic location (country and/or sea)

text: tree

text: uncultured prokaryote

text: uncultured prokaryote

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: tree and shrub savanna

characteristic: elevation

text: rangeland

text: Namibia

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: bulk

text: uncultured prokaryote

text: uncultured prokaryote


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: tree and shrub savanna

text: rangeland

text: Namibia

text: gras

characteristic: local environmental context

text: uncultured prokaryote

characteristic: project name

text: uncultured prokaryote


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: Submitter Id

text: tree and shrub savanna

characteristic: broad-scale environmental context

characteristic: collection date

text: low elefant density

text: Namibia

text: gras

text: uncultured prokaryote

characteristic: organism

text: uncultured prokaryote


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: tree and shrub savanna

characteristic: depth

text: high elow elefant densityefant density

characteristic: environmental medium

text: Namibia

characteristic: geographic location (country and/or sea)

text: bulk

text: uncultured prokaryote

text: uncultured prokaryote

characteristic: title


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: tree and shrub savanna

characteristic: elevation

text: low elefant density

text: Namibia

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: bulk

text: uncultured prokaryote

text: uncultured prokaryote


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Rueda-M,N

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Colombia

text: Linum tenue

text: Linum tenue

text: Female

characteristic: sex


text: Rueda-M,N

text: public

characteristic: Submitter Id

text: Colombia

text: Heliconius antiochus

characteristic: organism

text: Heliconius antiochus

text: Female


characteristic: External Id

text: Rueda-M,N

text: public

text: Colombia

text: Heliconius antiochus

text: Heliconius antiochus

text: Male


text: Rueda-M,N

text: public

text: Colombia

text: Heliconius antiochus

text: Heliconius antiochus

text: male


text: Rueda-M,N

text: public

characteristic: description

text: Venezuela

text: Heliconius antiochus

text: Heliconius antiochus

text: Male


text: Rueda-M,N

text: public

text: Venezuela

characteristic: geographic location (country and/or sea)

text: Heliconius antiochus

text: Heliconius antiochus

text: Female


text: Rueda-M,N

text: public

text: Colombia

text: Heliconius antiochus

text: Heliconius antiochus

text: Female

characteristic: title


text: Rueda-M,N

characteristic: INSDC center name

text: public

text: Colombia

text: Heliconius antiochus

text: Heliconius antiochus

text: Male


text: Rueda-M,N

text: public

text: Colombia

text: Heliconius congener

text: Heliconius congener

text: Male


text: Rueda-M,N

text: public

text: Ecuador

text: Heliconius congener

text: Heliconius congener

text: Female


text: Rueda-M,N

text: public

text: Ecuador

text: Heliconius congener

text: Heliconius congener

text: Male


text: Rueda-M,N

text: public

text: Panama

text: Heliconius eleuchia

text: Heliconius eleuchia

text: Female


text: Rueda-M,N

text: public

text: Colombia

text: Heliconius eleuchia

text: Heliconius eleuchia

text: Male


text: Rueda-M,N

text: public

text: Colombia

text: Heliconius eleuchia

text: Heliconius eleuchia

text: Female


text: Rueda-M,N

text: public

text: Ecuador

text: Heliconius eleuchia

text: Heliconius eleuchia

text: Male


text: Rueda-M,N

text: public

text: Ecuador

text: Heliconius eleuchia

text: Heliconius eleuchia

text: Female


text: Rueda-M,N

text: public

text: Panama

text: Heliconius hewitsoni

text: Heliconius hewitsoni

text: Male


text: Rueda-M,N

text: public

text: Panama

text: Heliconius hewitsoni

text: Heliconius hewitsoni

text: Female


text: Rueda-M,N

text: public

text: Peru

text: Heliconius leucadia

text: Heliconius leucadia


text: Rueda-M,N

text: public

text: Colombia

text: Heliconius leucadia

text: Heliconius leucadia

text: Female


text: Rueda-M,N

text: public

text: Colombia

text: Heliconius sapho

text: Heliconius sapho

text: Male


text: Rueda-M,N

text: public

text: Colombia

text: Heliconius sapho

text: Heliconius sapho

text: Female


text: Rueda-M,N

text: public

text: Panama

text: Heliconius sapho

text: Heliconius sapho

text: Male


text: Rueda-M,N

text: public

text: Panama

text: Heliconius sapho

text: Heliconius sapho

text: Female


text: Rueda-M,N

text: public

text: Colombia

text: Heliconius sara

text: Heliconius sara

text: Female


text: Rueda-M,N

text: public

text: Colombia

text: Heliconius sara

text: Heliconius sara

text: Male


text: Rueda-M,N

text: public

text: Panama

text: Heliconius sara

text: Heliconius sara

text: Male


text: Rueda-M,N

text: public

text: Venezuela

text: Heliconius sara

text: Heliconius sara

text: Male


text: Rueda-M,N

text: public

text: Brazil

text: Heliconius sara

text: Heliconius sara

text: Male


text: Rueda-M,N

text: public

text: Peru

text: Heliconius sara

text: Heliconius sara

text: Male


text: Rueda-M,N

text: public

text: Brazil

text: Heliconius sara

text: Heliconius sara

text: Female


text: Rueda-M,N

text: public

text: Peru

text: Heliconius sara

text: Heliconius sara

text: Female


text: Rueda-M,N

text: public

text: Ecuador

text: Heliconius sara

text: Heliconius sara

text: Female


text: Rueda-M,N

text: public

text: Ecuador

text: Heliconius sara

text: Heliconius sara

text: Male


text: Rueda-M,N

text: public

text: Suriname

text: Heliconius sara

text: Heliconius sara

text: Male


E-MTAB-12704:Sample 1

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC first public

text: public

text: E-MTAB-12704:Sample 1

tag: Namespace:UNIVERSITY OF MANCHESTER

unit: year

characteristic: age

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

text: Protocols: In vivo patch test of peptide combination: The peptide formulation was constituted by dissolving 1% w/w of a 50:50 mixture of P1 (500ppm) and P7 (500ppm) in solvent excipients added to a simple oil in water cosmetic emulsion containing water, glycerine and thickened with ammonium acryloyldimethyltaurate /VP Copolymer, xanthan gum and preserved with phenoxyethanolphenoxyethanol; giving a final total peptide concentrations of either 10ppm or 30ppm. A vehicle was also formulated with identical composition, including the excipients but without the peptides. Eight healthy, white but photoaged volunteers with visible signs of photoageing were recruited (Fitzpatrick skin phototype I-III; 3 male, 5 female, age range: 72-84 years old) and subjected to an extended 12-day patch test as described by PMID: 18070204. Only three of these volunteers were studied by RNA-seq here. The assay uses occlusion to carry actives into the skin, so mimicking long-term use PMID: 18070204. The peptide formulation was applied for twelve-days, with reapplication every 4-days, alongside a vehicle control and an occluded but untreated area (baseline). The vehicle and peptide formulations (30 μl) were applied separately to the extensor photoaged forearm under standard 6mm diameter Finn Chambers (Epitest Ltd). ATRA (0.025%; Retin-A) was applied to skin for the final 4-days only (to avoid potential complications of irritation from extended occlusion) as a positive control. On day 12, the Finn chambers were removed and 3mm punch biopsies obtained under 1% lignocaine local anaesthesia from each test site. Biopsies were embedded in optimal cutting temperature compound (Miles Laboratories, Elkhart, IN, USA), snap frozen in liquid nitrogen and stored at –80°C, prior to cutting at 10μm thickness. The study was conducted in accordance with the principles of the Declaration of Helsinki, with written informed consent (Manchester University Research Ethics Committee reference: 13677). Immunofluorescence analysis was performed to assess fibrillin microfibril abundance (a dermal repair marker). For extraction of biopsy RNA, once obtained, biopsies were immediately stored in RNAprotect Tissue Reagent (76104, Qiagen) to stabilise RNA. Biopsies were incubated at 37°C for one hour with 20mM EDTA, then the epidermis was separated from the dermis using forceps. Epidermal and dermal samples were then processed for RNA extraction using the RNeasy Fibrous Tissue Mini Kit (74704, Qiagen) according to manufacturer’s instructions. Briefly, samples were removed from the RNAprotect Tissue Reagent, and added to appropriate volumes of buffer RLT and homogenised at 50 Hz for 5 minutes using the TissueLyser LT (Qiagen), until all tissue was homogenised. Samples were then incubated with Proteinase K at 55°C for 10 minutes, prior to centrifugation to remove debris. A half-volume of ethanol was added, then samples were transferred to spin columns, where they were washed with buffer RW1. DNase I was then added to spin columns, and incubated at room temperature for 15 minutes. Samples underwent three further wash steps, once with buffer RW1, and the final two with buffer RPE. RNAse-free water was then used to elute RNA samples. Epidermal and dermal RNA samples were then combined and further processed to concentrate RNA and remove protein contaminants using RNeasy Mini Kit as above. Epidermal and dermal samples were then processed for RNA extraction using the RNeasy Fibrous Tissue Mini Kit (74704, Qiagen) according to manufacturer’s instructions. Briefly, samples were removed from the RNAprotect Tissue Reagent, and added to appropriate volumes of buffer RLT and homogenised at 50 Hz for 5 minutes using the TissueLyser LT (Qiagen), until all tissue was homogenised. Samples were then incubated with Proteinase K at 55°C for 10 minutes, prior to centrifugation to remove debris. A half-volume of ethanol was added, then samples were transferred to spin columns, where they were washed with buffer RW1. DNase I was then added to spin columns, and incubated at room temperature for 15 minutes. Samples underwent three further wash steps, once with buffer RW1, and the final two with buffer RPE. RNAse-free water was then used to elute RNA samples. Epidermal and dermal RNA samples were then combined and further processed to concentrate RNA and remove protein contaminants using RNeasy Mini Kit as above. Stranded mRNA assay (Illumina)

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: skin of forearm

characteristic: organism part

text: Homo sapiens

text: male

text: Sample 1


E-MTAB-12704:Sample 13

characteristic: ENA-CHECKLIST

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12704:Sample 13

characteristic: Submitter Id

text: ArrayExpress

text: human

text: adult

text: normal

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: skin of forearm

text: Homo sapiens

text: female

characteristic: sex

text: Sample 13


E-MTAB-12704:Sample 7

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

text: public

text: E-MTAB-12704:Sample 7

text: ArrayExpress

text: human

characteristic: description

text: adult

text: normal

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin of forearm

text: Homo sapiens

text: female

text: Sample 7

characteristic: title


E-MTAB-12704:Sample 15

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC first public

text: public

text: E-MTAB-12704:Sample 15

characteristic: age

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin of forearm

text: Homo sapiens

text: female

text: Sample 15


E-MTAB-12704:Sample 3

characteristic: ENA-CHECKLIST

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12704:Sample 3

text: ArrayExpress

text: human

text: adult

text: normal

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: skin of forearm

characteristic: organism part

text: Homo sapiens

text: male

text: Sample 3


E-MTAB-12704:Sample 9

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF MANCHESTER

text: public

characteristic: SRA accession

text: E-MTAB-12704:Sample 9

characteristic: Submitter Id

text: ArrayExpress

text: human

text: adult

text: normal

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: skin of forearm

text: Homo sapiens

text: female

characteristic: sex

text: Sample 9


E-MTAB-12704:Sample 12

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12704:Sample 12

characteristic: age

text: ArrayExpress

text: human

characteristic: description

text: adult

text: normal

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin of forearm

text: Homo sapiens

text: female

text: Sample 12

characteristic: title


E-MTAB-12704:Sample 18

characteristic: ENA-CHECKLIST

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC last update

text: public

text: E-MTAB-12704:Sample 18

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin of forearm

text: Homo sapiens

text: female

text: Sample 18


E-MTAB-12704:Sample 6

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF MANCHESTER

text: public

characteristic: INSDC status

text: E-MTAB-12704:Sample 6

text: ArrayExpress

text: human

text: adult

text: normal

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: skin of forearm

characteristic: organism part

text: Homo sapiens

text: male

text: Sample 6


E-MTAB-12704:Sample 11

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

text: public

characteristic: SRA accession

text: E-MTAB-12704:Sample 11

characteristic: Submitter Id

text: ArrayExpress

text: human

text: adult

text: normal

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: skin of forearm

text: Homo sapiens

text: female

characteristic: sex

text: Sample 11


E-MTAB-12704:Sample 17

characteristic: ENA-CHECKLIST

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-12704:Sample 17

characteristic: age

text: ArrayExpress

text: human

characteristic: description

text: adult

text: normal

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin of forearm

text: Homo sapiens

text: female

text: Sample 17

characteristic: title


E-MTAB-12704:Sample 5

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12704:Sample 5

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin of forearm

text: Homo sapiens

text: male

text: Sample 5


E-MTAB-12704:Sample 14

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: E-MTAB-12704:Sample 14

text: ArrayExpress

text: human

text: adult

text: normal

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: skin of forearm

characteristic: organism part

text: Homo sapiens

text: female

text: Sample 14


E-MTAB-12704:Sample 2

characteristic: ENA-CHECKLIST

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12704:Sample 2

characteristic: Submitter Id

text: ArrayExpress

text: human

text: adult

text: normal

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: skin of forearm

text: Homo sapiens

text: male

characteristic: sex

text: Sample 2


E-MTAB-12704:Sample 8

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC last update

text: public

text: E-MTAB-12704:Sample 8

characteristic: age

text: ArrayExpress

text: human

characteristic: description

text: adult

text: normal

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin of forearm

text: Homo sapiens

text: female

text: Sample 8

characteristic: title


characteristic: External Id

text: CEB

characteristic: INSDC center name

text: public

text: Soil before incubation

text: Soil

text: soil metagenome

text: soil metagenome

text: Soil Inoculum Reactor 1


text: CEB

text: public

characteristic: INSDC status

text: Soil before incubation

text: Soil

text: soil metagenome

text: soil metagenome

text: Soil Inoculum Reactor 2


text: CEB

text: public

text: Soil before incubation

text: Soil

text: soil metagenome

text: soil metagenome

text: Soil Inoculum Reactor 3


text: CEB

text: public

text: Soil after 180 days of incubation with PHB/PBAT films

text: Soil

text: soil metagenome

text: soil metagenome

text: Soil after biodegradation Reactor 1


text: CEB

text: public

text: Soil

text: soil metagenome

text: soil metagenome

text: Soil after biodegradation Reactor 2


text: CEB

text: public

text: Soil

text: soil metagenome

text: soil metagenome

text: Soil after biodegradation Reactor 3


OriginalSoil-Ieuk

text: CEB

text: public

text: OriginalSoil-Ieuk

text: Soil before incubation

text: Soil

text: soil metagenome

text: soil metagenome

text: Soil Inoculum


OriginalSoil-Iproc

text: CEB

text: public

text: OriginalSoil-Iproc

text: Soil before incubation

text: Soil

text: soil metagenome

text: soil metagenome

text: Soil Inoculum


ReactorA-Feuk

text: CEB

text: public

text: ReactorA-Feuk

text: Soil after 220 days of incubation with PHB/PBAT films

text: Soil

text: soil metagenome

text: soil metagenome

text: Soil after biodegradation Reactor 1


ReactorB-Feuk

text: CEB

text: public

text: ReactorB-Feuk

text: Soil

text: soil metagenome

text: soil metagenome

text: Soil after biodegradation Reactor 2


ReactorC-Feuk

text: CEB

text: public

text: ReactorC-Feuk

text: Soil

text: soil metagenome

text: soil metagenome

text: Soil after biodegradation Reactor 3


ReactorA-Fproc

text: CEB

text: public

text: ReactorA-Fproc

text: Soil

text: soil metagenome

text: soil metagenome

text: Soil after biodegradation Reactor 1


ReactorB-Fproc

text: CEB

text: public

text: ReactorB-Fproc

text: Soil

text: soil metagenome

text: soil metagenome

text: Soil after biodegradation Reactor 2


ReactorC-Fproc

text: CEB

text: public

text: ReactorC-Fproc

text: Soil

text: soil metagenome

text: soil metagenome

text: Soil after biodegradation Reactor 3


text: Plasmodium falciparum

characteristic: ArrayExpress-SPECIES

text: SC

text: public

text: malaria parasite P. falciparum

characteristic: common name

text: Plasmodium falciparum

text: Plasmodium falciparum


characteristic: ENA-CHECKLIST

text: Sanger Institute

characteristic: GAL

text: NOT PROVIDED

text: Tree of Life Programme

text: public

tag: Namespace:Tree of Life Programme

text: NORMAN LEE

text: ST OLAF COLLEGE

characteristic: collecting institution

characteristic: collection date

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: NORTHFIELD | MN | ST OLAF COLLEGE

characteristic: geographic location (region and locality)

text: LAB POPULATION

characteristic: habitat

text: NORMAN LEE

text: ST OLAF COLLEGE

text: adult

characteristic: lifestage

text: Ormia ochracea

text: WHOLE ORGANISM

characteristic: organism part

text: DTOL

characteristic: project name

text: Ormia ochracea

text: FEMALE

text: NOT PROVIDED

text: N

characteristic: symbiont

characteristic: tolid


E-MTAB-12730:Sample 1

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12730:Sample 1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: brain microvascular endothelial cell

text: human

text: Protocols: At each time point, the co-culture medium was removed, and the cells were washed once with EC medium to remove unbound IE or uninfected RBC. 1.5 ml of EC medium was added to each flask prior to harvesting the cells by cell-scraper. The cells were transferred to 1.5 ml sterile tubes and stored at -80°C. Malaria parasite culture. Laboratory-adapted IT4var14 (A4) [PMID: 1614515] and IT4var37 (4E12) [PMID: 21573138] P. falciparum isolates were cultured at 2% haematocrit in O+ human erythrocytes in complete RPMI 1640 medium (supplemented with 10% human serum, 37.5 mM HEPES, 11 mM D-glucose, 25 mg/ml gentamicin sulphate, 2 mM L-glutamine, pH 7.4) [PMID: 30103779]. IT4var14 binds to ICAM-1 and CD36, whereas IT4var37 is a strong CD36-binder but does not bind to ICAM-1 [PMID: 21573138]. IT4var14 binds to HUVEC, HBMEC and HDMEC. P. falciparum undergoes antigenic variation in culture, which changes the proportion of IE expressing specific PfEMP1s in the parasite populations. To minimize var gene switching, IT4var14 was selected with specific BC6 antibody [PMID: 1614515] coated protein G Dynabeads using standard protocols [PMID: 25360558]. IT4var37 does not have a specific antibody available for selection, but this variant is common [PMID: 21573138] and the presence of a significant proportion of this PfEMP1 expression was monitored by qRT-PCR using specific primers (ABRA6 – [PMID: 17878945]). The selected and screened parasite strains were used for experiments for up to 3 weeks. The parasite lines, EC cultures, washed blood cells and parasite culture media were regularly monitored for mycoplasma contamination using the Universal Mycoplasma Detection Kit (ATCC® 30-1012K™). Endothelial Cell culturing. Primary human brain microvascular endothelial cells (HBMEC) were obtained from Cell Systems, USA (ACBRI 376). HBMEC were cultured in 1% gelatin-coated flasks in Promocell’s Endothelial Cell Growth Medium MV (C-22020) supplemented with Endothelial Cell Growth Medium MV Supplement Mix (C-39225) and passaged using the Detach Kit (C-41220), following manufacturer’s instructions. Co-culture of EC with malaria parasite. HBMEC at passage 4 to 8 were cultured till 80-90% confluency in 25 cm2 flasks and, for +TNF conditions, stimulated with 10 ng/ml TNF overnight. Pf-IE were synchronised with 5% sorbitol, cultured until they reached 5-8% parasitaemia (mature stages) and enriched by Plasmion to obtain 50-60% parasitaemia. The enriched Pf-IE were resuspended in EC medium to a standard parasitemia of 50% at 1% haematocrit and overlayed onto the HBMEC for 0 (on-off), 2, 6 and 20 hours, in triplicate, in a 5% CO2 incubator. Uninfected red blood cells were used as a control. At each time point, the co-culture medium was removed, and the cells were washed once with EC medium to remove unbound IE or uninfected RBC. 1.5 ml of EC medium was added to each flask prior to harvesting the cells by cell-scraper. The cells were transferred to 1.5 ml sterile tubes and stored at -80°C. RNA was extracted using the RNeasy Mini Kit (Qiagen, 74104) following manufacturer’s instructions. The quality of the RNA samples was assessed using an Agilent 2100 Bioanalyzer (RNA 6000 Nano Kit, 5067-1511, Agilent Technologies) following manufacturer instructions. All RNA samples had a RIN of 8.5 or higher, indicating high-quality RNA. The quality of the RNA samples was assessed using an Agilent 2100 Bioanalyzer (RNA 6000 Nano Kit, 5067-1511, Agilent Technologies) following manufacturer instructions. All RNA samples had a RIN of 8.5 or higher, indicating high-quality RNA. Strand-specific RNA-sequencing (ssRNA-seq) libraries were prepared using the Illumina Truseq Stranded mRNA Sample Preparation Kit (Illumina, RS-122-2101) following the manufacturer's instructions.

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Plasmodium falciparum PfEMP1IT4var14

characteristic: infect

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: brain microvascular endothelium

text: Homo sapiens

unit: hour

characteristic: time

text: Sample 1

characteristic: title

unit: nanogram per milliliter

characteristic: tnf


E-MTAB-12730:Sample 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12730:Sample 2

text: ArrayExpress

text: brain microvascular endothelial cell

text: human

characteristic: common name

text: adult

text: wild type genotype

text: Plasmodium falciparum PfEMP1IT4var14

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: brain microvascular endothelium

characteristic: organism part

text: Homo sapiens

text: Sample 2


E-MTAB-12730:Sample 3

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12730:Sample 3

characteristic: Submitter Id

text: ArrayExpress

text: brain microvascular endothelial cell

text: human

text: adult

text: wild type genotype

text: Plasmodium falciparum PfEMP1IT4var14

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: brain microvascular endothelium

text: Homo sapiens

text: Sample 3


E-MTAB-12730:Sample 4

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12730:Sample 4

text: ArrayExpress

characteristic: broker name

text: brain microvascular endothelial cell

text: human

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Plasmodium falciparum PfEMP1IT4var14

characteristic: infect

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: brain microvascular endothelium

text: Homo sapiens

text: Sample 4


E-MTAB-12730:Sample 5

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12730:Sample 5

text: ArrayExpress

text: brain microvascular endothelial cell

text: human

text: adult

text: wild type genotype

text: Plasmodium falciparum PfEMP1IT4var14

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: brain microvascular endothelium

text: Homo sapiens

characteristic: time

text: Sample 5

characteristic: title

characteristic: tnf


E-MTAB-12730:Sample 6

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12730:Sample 6

text: ArrayExpress

text: brain microvascular endothelial cell

text: human

characteristic: common name

text: adult

text: wild type genotype

text: Plasmodium falciparum PfEMP1IT4var14

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: brain microvascular endothelium

characteristic: organism part

text: Homo sapiens

text: Sample 6


E-MTAB-12730:Sample 7

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12730:Sample 7

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: brain microvascular endothelial cell

text: human

characteristic: description

text: adult

text: wild type genotype

text: Plasmodium falciparum PfEMP1IT4var14

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: brain microvascular endothelium

text: Homo sapiens

text: Sample 7


E-MTAB-12730:Sample 8

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12730:Sample 8

text: ArrayExpress

text: brain microvascular endothelial cell

text: human

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Plasmodium falciparum PfEMP1IT4var14

characteristic: infect

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: brain microvascular endothelium

text: Homo sapiens

text: Sample 8


E-MTAB-12730:Sample 41

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12730:Sample 41

text: ArrayExpress

text: erythrocyte

text: human

text: adult

text: wild type genotype

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: Homo sapiens

characteristic: time

text: Sample 41

characteristic: title

characteristic: tnf


E-MTAB-12730:Sample 42

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12730:Sample 42

text: ArrayExpress

text: erythrocyte

text: human

characteristic: common name

text: adult

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens

text: Sample 42


E-MTAB-12730:Sample 43

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

text: public

characteristic: SRA accession

text: E-MTAB-12730:Sample 43

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: erythrocyte

text: human

characteristic: description

text: adult

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 43


E-MTAB-12730:Sample 44

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12730:Sample 44

text: ArrayExpress

text: erythrocyte

text: human

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

characteristic: infect

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 44


E-MTAB-12730:Sample 45

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12730:Sample 45

text: ArrayExpress

text: erythrocyte

text: human

text: adult

text: wild type genotype

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: Homo sapiens

characteristic: time

text: Sample 45

characteristic: title

characteristic: tnf


E-MTAB-12730:Sample 46

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12730:Sample 46

text: ArrayExpress

text: erythrocyte

text: human

characteristic: common name

text: adult

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens

text: Sample 46


E-MTAB-12730:Sample 22

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

text: public

characteristic: SRA accession

text: E-MTAB-12730:Sample 22

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: brain microvascular endothelial cell

text: human

characteristic: description

text: adult

text: wild type genotype

text: Plasmodium falciparum PfEMP1IT4var14

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: brain microvascular endothelium

text: Homo sapiens

text: Sample 22


E-MTAB-12730:Sample 23

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12730:Sample 23

text: ArrayExpress

text: brain microvascular endothelial cell

text: human

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Plasmodium falciparum PfEMP1IT4var14

characteristic: infect

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: brain microvascular endothelium

text: Homo sapiens

text: Sample 23


E-MTAB-12730:Sample 24

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12730:Sample 24

text: ArrayExpress

text: brain microvascular endothelial cell

text: human

text: adult

text: wild type genotype

text: Plasmodium falciparum PfEMP1IT4var14

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: brain microvascular endothelium

text: Homo sapiens

characteristic: time

text: Sample 24

characteristic: title

characteristic: tnf


E-MTAB-12730:Sample 25

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12730:Sample 25

text: ArrayExpress

text: brain microvascular endothelial cell

text: human

characteristic: common name

text: adult

text: wild type genotype

text: Plasmodium falciparum PfEMP1IT4var14

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: brain microvascular endothelium

characteristic: organism part

text: Homo sapiens

text: Sample 25


E-MTAB-12730:Sample 28

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12730:Sample 28

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: brain microvascular endothelial cell

text: human

characteristic: description

text: adult

text: wild type genotype

text: Plasmodium falciparum PfEMP1IT4var14

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: brain microvascular endothelium

text: Homo sapiens

text: Sample 28


E-MTAB-12730:Sample 29

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12730:Sample 29

text: ArrayExpress

text: brain microvascular endothelial cell

text: human

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Plasmodium falciparum PfEMP1IT4var14

characteristic: infect

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: brain microvascular endothelium

text: Homo sapiens

text: Sample 29


E-MTAB-12730:Sample 30

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12730:Sample 30

text: ArrayExpress

text: brain microvascular endothelial cell

text: human

text: adult

text: wild type genotype

text: Plasmodium falciparum PfEMP1IT4var14

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: brain microvascular endothelium

text: Homo sapiens

characteristic: time

text: Sample 30

characteristic: title

characteristic: tnf


E-MTAB-12730:Sample 35

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12730:Sample 35

text: ArrayExpress

text: brain microvascular endothelial cell

text: human

characteristic: common name

text: adult

text: wild type genotype

text: Plasmodium falciparum PfEMP1IT4var14

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: brain microvascular endothelium

characteristic: organism part

text: Homo sapiens

text: Sample 35


E-MTAB-12730:Sample 36

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

text: public

characteristic: SRA accession

text: E-MTAB-12730:Sample 36

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: brain microvascular endothelial cell

text: human

characteristic: description

text: adult

text: wild type genotype

text: Plasmodium falciparum PfEMP1IT4var14

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: brain microvascular endothelium

text: Homo sapiens

text: Sample 36


E-MTAB-12730:Sample 37

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12730:Sample 37

text: ArrayExpress

text: brain microvascular endothelial cell

text: human

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Plasmodium falciparum PfEMP1IT4var14

characteristic: infect

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: brain microvascular endothelium

text: Homo sapiens

text: Sample 37


E-MTAB-12730:Sample 38

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12730:Sample 38

text: ArrayExpress

text: brain microvascular endothelial cell

text: human

text: adult

text: wild type genotype

text: Plasmodium falciparum PfEMP1IT4var14

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: brain microvascular endothelium

text: Homo sapiens

characteristic: time

text: Sample 38

characteristic: title

characteristic: tnf


E-MTAB-12730:Sample 10

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12730:Sample 10

text: ArrayExpress

text: brain microvascular endothelial cell

text: human

characteristic: common name

text: adult

text: wild type genotype

text: Plasmodium falciparum PfEMP1IT4var37

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: brain microvascular endothelium

characteristic: organism part

text: Homo sapiens

text: Sample 10


E-MTAB-12730:Sample 11

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

text: public

characteristic: SRA accession

text: E-MTAB-12730:Sample 11

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: brain microvascular endothelial cell

text: human

characteristic: description

text: adult

text: wild type genotype

text: Plasmodium falciparum PfEMP1IT4var37

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: brain microvascular endothelium

text: Homo sapiens

text: Sample 11


E-MTAB-12730:Sample 12

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12730:Sample 12

text: ArrayExpress

text: brain microvascular endothelial cell

text: human

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Plasmodium falciparum PfEMP1IT4var37

characteristic: infect

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: brain microvascular endothelium

text: Homo sapiens

text: Sample 12


E-MTAB-12730:Sample 13

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12730:Sample 13

text: ArrayExpress

text: brain microvascular endothelial cell

text: human

text: adult

text: wild type genotype

text: Plasmodium falciparum PfEMP1IT4var37

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: brain microvascular endothelium

text: Homo sapiens

characteristic: time

text: Sample 13

characteristic: title

characteristic: tnf


E-MTAB-12730:Sample 14

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12730:Sample 14

text: ArrayExpress

text: brain microvascular endothelial cell

text: human

characteristic: common name

text: adult

text: wild type genotype

text: Plasmodium falciparum PfEMP1IT4var37

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: brain microvascular endothelium

characteristic: organism part

text: Homo sapiens

text: Sample 14


E-MTAB-12730:Sample 15

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12730:Sample 15

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: brain microvascular endothelial cell

text: human

characteristic: description

text: adult

text: wild type genotype

text: Plasmodium falciparum PfEMP1IT4var37

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: brain microvascular endothelium

text: Homo sapiens

text: Sample 15


E-MTAB-12730:Sample 16

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12730:Sample 16

text: ArrayExpress

text: brain microvascular endothelial cell

text: human

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Plasmodium falciparum PfEMP1IT4var37

characteristic: infect

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: brain microvascular endothelium

text: Homo sapiens

text: Sample 16


E-MTAB-12730:Sample 17

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12730:Sample 17

text: ArrayExpress

text: brain microvascular endothelial cell

text: human

text: adult

text: wild type genotype

text: Plasmodium falciparum PfEMP1IT4var37

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: brain microvascular endothelium

text: Homo sapiens

characteristic: time

text: Sample 17

characteristic: title

characteristic: tnf


E-MTAB-12730:Sample 9

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12730:Sample 9

text: ArrayExpress

text: brain microvascular endothelial cell

text: human

characteristic: common name

text: adult

text: wild type genotype

text: Plasmodium falciparum PfEMP1IT4var37

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: brain microvascular endothelium

characteristic: organism part

text: Homo sapiens

text: Sample 9


E-MTAB-12730:Sample 18

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

text: public

characteristic: SRA accession

text: E-MTAB-12730:Sample 18

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: erythrocyte

text: human

characteristic: description

text: adult

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 18


E-MTAB-12730:Sample 19

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12730:Sample 19

text: ArrayExpress

text: erythrocyte

text: human

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

characteristic: infect

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 19


E-MTAB-12730:Sample 20

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12730:Sample 20

text: ArrayExpress

text: erythrocyte

text: human

text: adult

text: wild type genotype

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: Homo sapiens

characteristic: time

text: Sample 20

characteristic: title

characteristic: tnf


E-MTAB-12730:Sample 21

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12730:Sample 21

text: ArrayExpress

text: erythrocyte

text: human

characteristic: common name

text: adult

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens

text: Sample 21


E-MTAB-12730:Sample 26

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

text: public

characteristic: SRA accession

text: E-MTAB-12730:Sample 26

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: erythrocyte

text: human

characteristic: description

text: adult

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 26


E-MTAB-12730:Sample 27

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12730:Sample 27

text: ArrayExpress

text: erythrocyte

text: human

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

characteristic: infect

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 27


E-MTAB-12730:Sample 31

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12730:Sample 31

text: ArrayExpress

text: erythrocyte

text: human

text: adult

text: wild type genotype

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: Homo sapiens

characteristic: time

text: Sample 31

characteristic: title

characteristic: tnf


E-MTAB-12730:Sample 32

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12730:Sample 32

text: ArrayExpress

text: erythrocyte

text: human

characteristic: common name

text: adult

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens

text: Sample 32


E-MTAB-12730:Sample 33

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12730:Sample 33

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: erythrocyte

text: human

characteristic: description

text: adult

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 33


E-MTAB-12730:Sample 34

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12730:Sample 34

text: ArrayExpress

text: erythrocyte

text: human

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

characteristic: infect

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 34


E-MTAB-12730:Sample 39

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12730:Sample 39

text: ArrayExpress

text: erythrocyte

text: human

text: adult

text: wild type genotype

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: Homo sapiens

characteristic: time

text: Sample 39

characteristic: title

characteristic: tnf


E-MTAB-12730:Sample 40

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12730:Sample 40

text: ArrayExpress

text: erythrocyte

text: human

characteristic: common name

text: adult

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens

text: Sample 40


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: KLOTOE

text: public

characteristic: SRA accession

text: ena-SAMPLE-TAB-07-03-2023-12:34:13:108-13526

characteristic: Submitter Id

text: DAVAR

characteristic: collection date

text: Bacteria gram negative

characteristic: description

text: New Caledonia

characteristic: geographic location (country and/or sea)

text: Peninsula

characteristic: geographic location (region and locality)

text: diseased

characteristic: host health state

text: Goat

characteristic: host scientific name

text: Tissues from pleural, testicular abscesses

text: -21.8° or 21° 48' south 165.76667° or 165° 46' east

text: Burkholderia pseudomallei

text: Burkholderia pseudomallei

text: not provided

characteristic: serovar

characteristic: strain


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

tag: Namespace:Department of Medicine 1, Friedrich Alexander University, Erlangen-Nuremberg, Germany

unit: week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

text: Protocols: Mice were sacrificed and its distal colon was collected Total RNA was extracted from the samples taken from colons of mice using the peqGOLD total RNA kit (Peqlab/VWR) following the manufacturers' instructions. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library.The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on Illumina platforms, according to effective library concentration and data amount.

text: adult

characteristic: developmental stage

text: colitis

characteristic: disease

text: IL20 -/-

characteristic: genotype

text: not applicable

characteristic: isolate

text: Mus musculus

characteristic: organism

text: Mus musculus

text: colon

text: distal colon

characteristic: sampling site

text: Mus musculus

text: male

characteristic: sex

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Department of Medicine 1, Friedrich Alexander University, Erlangen-Nuremberg, Germany

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: colitis

text: IL20 -/-

text: not applicable

text: Mus musculus

text: Mus musculus

text: colon

characteristic: organism part

text: distal colon

text: Mus musculus

text: male


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

text: adult

characteristic: developmental stage

text: colitis

text: IL20ra -/-

text: not applicable

text: Mus musculus

text: Mus musculus

text: colon

text: distal colon

text: Mus musculus

text: male

characteristic: strain


characteristic: ENA-CHECKLIST

text: public

text: ArrayExpress

text: house mouse

text: adult

text: colitis

characteristic: disease

text: wild type genotype

characteristic: genotype

text: not applicable

characteristic: isolate

text: Mus musculus

characteristic: organism

text: Mus musculus

text: colon

text: distal colon

characteristic: sampling site

text: Mus musculus

text: male

characteristic: sex

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:The Blizard Institute Barts and The London School of Medicine and Dentistry, Queen Mary, University of London

characteristic: Submitter Id

text: ArrayExpress

text: T cell

text: house mouse

characteristic: common name

text: Protocols: CD4+CD25-CD44hiFR4+Egr2+ cells were isolated from Egr2 Kin mice by FACS RNA was isolated and purified using the RNeasy UCP Micro Kit (Qiagen) according to the manufacturer's instructions RNA from T cells was processed for directional mRNA-Seq library construction using the KAPA mRNA HyperPrep Kit (Kapa Biosystems) in combination with IDT xGen UDI-UMI adapters (Integrated DNA Technologies) according to the manufacturers’ protocols

characteristic: description

text: adult

text: GFP-Egr2 knockin

characteristic: immunophenotype

text: mixed pool from 10 mice

characteristic: individual

characteristic: infect

text: not applicable

text: Mus musculus

text: Mus musculus

text: mixed lymph node and spleen

characteristic: organism part

text: Mus musculus

text: mixed


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Blizard Institute Barts and The London School of Medicine and Dentistry, Queen Mary, University of London

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: age

text: ArrayExpress

characteristic: broker name

text: T cell

text: house mouse

text: adult

characteristic: developmental stage

text: GFP-Egr2 knockin

characteristic: genotype

text: mixed pool from 10 mice

text: not applicable

text: Mus musculus

text: Mus musculus

text: mixed lymph node and spleen

text: Mus musculus

text: mixed

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: T cell

text: house mouse

text: Protocols: CD4+CD25-CD44hiFR4+Egr2- cells were isolated from Egr2 Kin mice by FACS RNA was isolated and purified using the RNeasy UCP Micro Kit (Qiagen) according to the manufacturer's instructions RNA from T cells was processed for directional mRNA-Seq library construction using the KAPA mRNA HyperPrep Kit (Kapa Biosystems) in combination with IDT xGen UDI-UMI adapters (Integrated DNA Technologies) according to the manufacturers’ protocols

text: adult

text: GFP-Egr2 knockin

text: mixed pool from 10 mice

text: not applicable

characteristic: isolate

text: Mus musculus

characteristic: organism

text: Mus musculus

text: mixed lymph node and spleen

text: Mus musculus

text: mixed

characteristic: sex

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: ArrayExpress

text: T cell

text: house mouse

characteristic: common name

text: Protocols: CD4+CD25-CD44hiFR4-Egr2- cells were isolated from Egr2 Kin mice by FACS RNA was isolated and purified using the RNeasy UCP Micro Kit (Qiagen) according to the manufacturer's instructions RNA from T cells was processed for directional mRNA-Seq library construction using the KAPA mRNA HyperPrep Kit (Kapa Biosystems) in combination with IDT xGen UDI-UMI adapters (Integrated DNA Technologies) according to the manufacturers’ protocols

characteristic: description

text: adult

text: GFP-Egr2 knockin

characteristic: immunophenotype

text: mixed pool from 10 mice

characteristic: individual

characteristic: infect

text: not applicable

text: Mus musculus

text: Mus musculus

text: mixed lymph node and spleen

characteristic: organism part

text: Mus musculus

text: mixed


characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: age

text: ArrayExpress

characteristic: broker name

text: T cell

text: house mouse

text: Protocols: CD4+CD25-CD44hiFR4+Egr2-/-Egr3-/- cells were isolated from Egr2/3 DKO mice by FACS GFP-Egr2 knockin (Egr2 Kin) and hCD2-Cre Egr2loxP/loxP Egr3-/- (Egr2/3 DKO) mice were infected intraperitoneally with 4 x 10^6 PFU of vaccinia virus. CD4 T cells were isolated 21 days after infection RNA was isolated and purified using the RNeasy UCP Micro Kit (Qiagen) according to the manufacturer's instructions RNA from T cells was processed for directional mRNA-Seq library construction using the KAPA mRNA HyperPrep Kit (Kapa Biosystems) in combination with IDT xGen UDI-UMI adapters (Integrated DNA Technologies) according to the manufacturers’ protocols

text: adult

characteristic: developmental stage

text: hCD2-Cre Egr2loxP/loxP Egr3-/-

characteristic: genotype

text: mixed pool from 10 mice

text: vaccinia virus

text: not applicable

text: Mus musculus

text: Mus musculus

text: mixed lymph node and spleen

text: Mus musculus

text: mixed

characteristic: strain