characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: Pseudomonas lundensis

characteristic: organism

text: Pseudomonas lundensis

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas;s__Pseudomonas lundensis

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968817_bin.12_MetaWRAP_v1.3_MAG


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968817 of study ERP139515.

text: plaque biofilm

characteristic: environmental medium

text: Australia

unit: DD

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: uncultured Microbacterium sp.

text: Antibiotic overuse has promoted the spread of antimicrobial resistance (AMR) with significant health and economic consequences. Surveillance of resistance reservoirs, like the rarely explored oral microbiome, is necessary to combat AMR. We characterised the development of the paediatric oral resistome and investigated its role in dental caries in 221 twin children sampled over the first decade of life. From 535 oral metagenomes, we identified 309 antimicrobial resistance genes (ARGs), which significantly clustered by age, with the influence of host genetics on antimicrobial resistance increasing over time. Our results suggest the mobilisation potential of ARGs increased with age as Tn916 transposase was co-located with more species and ARGs as children grew. Dental caries compared to health was associated with a depletion of ARGs and species, which was reversed when restored. Our results highlight the dynamic nature of the paediatric oral resistome, and its potential role in transmission of AMR and dysbiosis.

characteristic: project name

characteristic: sample derived from

text: uncultured Microbacterium sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Actinobacteria;c__Actinomycetia;o__Micrococcales;f__Microbacteriaceae;g__Microbacterium;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968817_bin.19_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: Paenarthrobacter ilicis

characteristic: organism

text: Paenarthrobacter ilicis

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Actinobacteria;c__Actinomycetia;o__Micrococcales;f__Micrococcaceae;g__Paenarthrobacter;s__Paenarthrobacter ilicis

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968817_bin.5_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968818 of study ERP139515.

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: Neisseria lactamica

characteristic: project name

characteristic: sample derived from

text: Neisseria lactamica

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Betaproteobacteria;o__Neisseriales;f__Neisseriaceae;g__Neisseria;s__Neisseria lactamica

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968818_bin.1_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: Rothia dentocariosa

characteristic: organism

text: Rothia dentocariosa

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Actinobacteria;c__Actinomycetia;o__Micrococcales;f__Micrococcaceae;g__Rothia;s__Rothia dentocariosa

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968818_bin.8_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968819 of study ERP139515.

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: Acinetobacter lwoffii

characteristic: project name

characteristic: sample derived from

text: Acinetobacter lwoffii

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter;s__Acinetobacter lwoffii

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968819_bin.21_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: uncultured Porphyromonadaceae bacterium

characteristic: organism

text: uncultured Porphyromonadaceae bacterium

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Porphyromonadaceae;g__;s__

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968819_bin.26_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: Capnocytophaga sputigena

characteristic: project name

characteristic: sample derived from

text: Capnocytophaga sputigena

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Flavobacteriia;o__Flavobacteriales;f__Flavobacteriaceae;g__Capnocytophaga;s__Capnocytophaga sputigena

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968819_bin.7_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968820 of study ERP139515.

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: Streptococcus oralis

characteristic: organism

text: Antibiotic overuse has promoted the spread of antimicrobial resistance (AMR) with significant health and economic consequences. Surveillance of resistance reservoirs, like the rarely explored oral microbiome, is necessary to combat AMR. We characterised the development of the paediatric oral resistome and investigated its role in dental caries in 221 twin children sampled over the first decade of life. From 535 oral metagenomes, we identified 309 antimicrobial resistance genes (ARGs), which significantly clustered by age, with the influence of host genetics on antimicrobial resistance increasing over time. Our results suggest the mobilisation potential of ARGs increased with age as Tn916 transposase was co-located with more species and ARGs as children grew. Dental caries compared to health was associated with a depletion of ARGs and species, which was reversed when restored. Our results highlight the dynamic nature of the paediatric oral resistome, and its potential role in transmission of AMR and dysbiosis.

text: Streptococcus oralis

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Streptococcus;s__Streptococcus oralis

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968820_bin.25_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968821 of study ERP139515.

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: uncultured Candidatus Saccharimonas sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Candidatus Saccharimonas sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Candidatus Saccharibacteria;c__;o__;f__;g__Candidatus Saccharimonas;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968821_bin.16_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: Actinomyces oris

characteristic: organism

text: Actinomyces oris

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Actinobacteria;c__Actinomycetia;o__Actinomycetales;f__Actinomycetaceae;g__Actinomyces;s__Actinomyces oris

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968821_bin.23_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: uncultured Flavobacteriaceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Flavobacteriaceae bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Flavobacteriia;o__Flavobacteriales;f__Flavobacteriaceae;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968821_bin.31_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: Leptotrichia massiliensis

characteristic: organism

text: Leptotrichia massiliensis

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Fusobacteria;c__Fusobacteriia;o__Fusobacteriales;f__Leptotrichiaceae;g__Leptotrichia;s__Leptotrichia massiliensis

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968821_bin.6_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968822 of study ERP139515.

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: uncultured Leptotrichia sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Leptotrichia sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Fusobacteria;c__Fusobacteriia;o__Fusobacteriales;f__Leptotrichiaceae;g__Leptotrichia;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968822_bin.16_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: uncultured Candidatus Gracilibacteria bacterium

characteristic: organism

text: Antibiotic overuse has promoted the spread of antimicrobial resistance (AMR) with significant health and economic consequences. Surveillance of resistance reservoirs, like the rarely explored oral microbiome, is necessary to combat AMR. We characterised the development of the paediatric oral resistome and investigated its role in dental caries in 221 twin children sampled over the first decade of life. From 535 oral metagenomes, we identified 309 antimicrobial resistance genes (ARGs), which significantly clustered by age, with the influence of host genetics on antimicrobial resistance increasing over time. Our results suggest the mobilisation potential of ARGs increased with age as Tn916 transposase was co-located with more species and ARGs as children grew. Dental caries compared to health was associated with a depletion of ARGs and species, which was reversed when restored. Our results highlight the dynamic nature of the paediatric oral resistome, and its potential role in transmission of AMR and dysbiosis.

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Candidatus Gracilibacteria;c__;o__;f__;g__;s__

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968822_bin.3_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968823 of study ERP139515.

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: uncultured Candidatus Saccharibacteria bacterium

characteristic: project name

characteristic: sample derived from

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Candidatus Saccharibacteria;c__Candidatus Saccharimonia;o__Candidatus Nanosynbacterales;f__Candidatus Nanosynbacteraceae;g__Candidatus Nanosynbacter;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968823_bin.13_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: Yersinia massiliensis

characteristic: organism

text: Yersinia massiliensis

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Yersiniaceae;g__Yersinia;s__Yersinia massiliensis

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968823_bin.14_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: Stenotrophomonas maltophilia

characteristic: project name

characteristic: sample derived from

text: Stenotrophomonas maltophilia

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Stenotrophomonas;s__Stenotrophomonas maltophilia

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968823_bin.2_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: Comamonas testosteroni

characteristic: organism

text: Comamonas testosteroni

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Betaproteobacteria;o__Burkholderiales;f__Comamonadaceae;g__Comamonas;s__Comamonas testosteroni

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968823_bin.26_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: Pseudomonas alcaligenes

characteristic: project name

characteristic: sample derived from

text: Pseudomonas alcaligenes

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas;s__Pseudomonas alcaligenes

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968823_bin.27_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text: Antibiotic overuse has promoted the spread of antimicrobial resistance (AMR) with significant health and economic consequences. Surveillance of resistance reservoirs, like the rarely explored oral microbiome, is necessary to combat AMR. We characterised the development of the paediatric oral resistome and investigated its role in dental caries in 221 twin children sampled over the first decade of life. From 535 oral metagenomes, we identified 309 antimicrobial resistance genes (ARGs), which significantly clustered by age, with the influence of host genetics on antimicrobial resistance increasing over time. Our results suggest the mobilisation potential of ARGs increased with age as Tn916 transposase was co-located with more species and ARGs as children grew. Dental caries compared to health was associated with a depletion of ARGs and species, which was reversed when restored. Our results highlight the dynamic nature of the paediatric oral resistome, and its potential role in transmission of AMR and dysbiosis.

text: uncultured Lachnospiraceae bacterium

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Eubacteriales;f__Lachnospiraceae;g__;s__

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968823_bin.30_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968823 of study ERP139515.

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: uncultured Enterobacteriaceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Enterobacteriaceae bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968823_bin.38_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: uncultured Psychrobacter sp.

characteristic: organism

text: uncultured Psychrobacter sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Psychrobacter;s__

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968823_bin.9_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968825 of study ERP139515.

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: uncultured Neglectibacter sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Neglectibacter sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Eubacteriales;f__;g__Neglecta;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968825_bin.11_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text: uncultured Lachnospiraceae bacterium

text: Illumina NovaSeq 6000

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968825_bin.14_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: uncultured Anaerovorax sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Anaerovorax sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Eubacteriales;f__;g__Anaerovorax;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968825_bin.21_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: uncultured bacterium

characteristic: organism

text: uncultured bacterium

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__;c__;o__;f__;g__;s__

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968825_bin.3_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: Rouxiella badensis

text: Antibiotic overuse has promoted the spread of antimicrobial resistance (AMR) with significant health and economic consequences. Surveillance of resistance reservoirs, like the rarely explored oral microbiome, is necessary to combat AMR. We characterised the development of the paediatric oral resistome and investigated its role in dental caries in 221 twin children sampled over the first decade of life. From 535 oral metagenomes, we identified 309 antimicrobial resistance genes (ARGs), which significantly clustered by age, with the influence of host genetics on antimicrobial resistance increasing over time. Our results suggest the mobilisation potential of ARGs increased with age as Tn916 transposase was co-located with more species and ARGs as children grew. Dental caries compared to health was associated with a depletion of ARGs and species, which was reversed when restored. Our results highlight the dynamic nature of the paediatric oral resistome, and its potential role in transmission of AMR and dysbiosis.

characteristic: project name

characteristic: sample derived from

text: Rouxiella badensis

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Yersiniaceae;g__Rouxiella;s__Rouxiella badensis

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968825_bin.6_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: Aliarcobacter butzleri

characteristic: organism

text: Aliarcobacter butzleri

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Epsilonproteobacteria;o__Campylobacterales;f__Campylobacteraceae;g__Aliarcobacter;s__Aliarcobacter butzleri

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968825_bin.8_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968826 of study ERP139515.

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: Prevotella conceptionensis

characteristic: project name

characteristic: sample derived from

text: Prevotella conceptionensis

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Prevotellaceae;g__Prevotella;s__Prevotella conceptionensis

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968826_bin.12_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: uncultured Candidatus Saccharibacteria bacterium

characteristic: organism

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Candidatus Saccharibacteria;c__;o__;f__;g__;s__

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968826_bin.8_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968827 of study ERP139515.

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: uncultured Enterobacter sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Enterobacter sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Enterobacter;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968827_bin.2_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: uncultured Veillonella sp.

characteristic: organism

text: uncultured Veillonella sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Negativicutes;o__Veillonellales;f__Veillonellaceae;g__Veillonella;s__

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968827_bin.6_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: Prevotella melaninogenica

text: Antibiotic overuse has promoted the spread of antimicrobial resistance (AMR) with significant health and economic consequences. Surveillance of resistance reservoirs, like the rarely explored oral microbiome, is necessary to combat AMR. We characterised the development of the paediatric oral resistome and investigated its role in dental caries in 221 twin children sampled over the first decade of life. From 535 oral metagenomes, we identified 309 antimicrobial resistance genes (ARGs), which significantly clustered by age, with the influence of host genetics on antimicrobial resistance increasing over time. Our results suggest the mobilisation potential of ARGs increased with age as Tn916 transposase was co-located with more species and ARGs as children grew. Dental caries compared to health was associated with a depletion of ARGs and species, which was reversed when restored. Our results highlight the dynamic nature of the paediatric oral resistome, and its potential role in transmission of AMR and dysbiosis.

characteristic: project name

characteristic: sample derived from

text: Prevotella melaninogenica

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Prevotellaceae;g__Prevotella;s__Prevotella melaninogenica

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968827_bin.7_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: Pseudomonas fragi

characteristic: organism

text: Pseudomonas fragi

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas;s__Pseudomonas fragi

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968827_bin.8_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968828 of study ERP139515.

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: Leptotrichia hongkongensis

characteristic: project name

characteristic: sample derived from

text: Leptotrichia hongkongensis

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Fusobacteria;c__Fusobacteriia;o__Fusobacteriales;f__Leptotrichiaceae;g__Leptotrichia;s__Leptotrichia hongkongensis

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968828_bin.13_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: uncultured Capnocytophaga sp.

characteristic: organism

text: uncultured Capnocytophaga sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Flavobacteriia;o__Flavobacteriales;f__Flavobacteriaceae;g__Capnocytophaga;s__

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968828_bin.14_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: Pseudomonas entomophila

characteristic: project name

characteristic: sample derived from

text: Pseudomonas entomophila

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas;s__Pseudomonas entomophila

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968828_bin.4_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968829 of study ERP139515.

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: uncultured Neisseria sp.

characteristic: organism

text: uncultured Neisseria sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Betaproteobacteria;o__Neisseriales;f__Neisseriaceae;g__Neisseria;s__

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968829_bin.15_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: Capnocytophaga leadbetteri

characteristic: project name

characteristic: sample derived from

text: Capnocytophaga leadbetteri

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Flavobacteriia;o__Flavobacteriales;f__Flavobacteriaceae;g__Capnocytophaga;s__Capnocytophaga leadbetteri

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968829_bin.2_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: Streptococcus sanguinis

characteristic: organism

text: Antibiotic overuse has promoted the spread of antimicrobial resistance (AMR) with significant health and economic consequences. Surveillance of resistance reservoirs, like the rarely explored oral microbiome, is necessary to combat AMR. We characterised the development of the paediatric oral resistome and investigated its role in dental caries in 221 twin children sampled over the first decade of life. From 535 oral metagenomes, we identified 309 antimicrobial resistance genes (ARGs), which significantly clustered by age, with the influence of host genetics on antimicrobial resistance increasing over time. Our results suggest the mobilisation potential of ARGs increased with age as Tn916 transposase was co-located with more species and ARGs as children grew. Dental caries compared to health was associated with a depletion of ARGs and species, which was reversed when restored. Our results highlight the dynamic nature of the paediatric oral resistome, and its potential role in transmission of AMR and dysbiosis.

text: Streptococcus sanguinis

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Streptococcus;s__Streptococcus sanguinis

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968829_bin.5_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968830 of study ERP139515.

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: uncultured Gemella sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Gemella sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales;f__;g__Gemella;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968830_bin.13_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968831 of study ERP139515.

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: uncultured Neisseria sp.

characteristic: organism

text: uncultured Neisseria sp.

text: Illumina NovaSeq 6000

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968831_bin.4_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968832 of study ERP139515.

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: uncultured Streptococcus sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Streptococcus sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Streptococcus;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968832_bin.11_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: uncultured Leptotrichia sp.

characteristic: organism

text: uncultured Leptotrichia sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Fusobacteria;c__Fusobacteriia;o__Fusobacteriales;f__Leptotrichiaceae;g__Leptotrichia;s__

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968832_bin.17_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: uncultured Kingella sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Kingella sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Betaproteobacteria;o__Neisseriales;f__Neisseriaceae;g__Kingella;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968832_bin.30_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: Haemophilus haemolyticus

characteristic: organism

text: Antibiotic overuse has promoted the spread of antimicrobial resistance (AMR) with significant health and economic consequences. Surveillance of resistance reservoirs, like the rarely explored oral microbiome, is necessary to combat AMR. We characterised the development of the paediatric oral resistome and investigated its role in dental caries in 221 twin children sampled over the first decade of life. From 535 oral metagenomes, we identified 309 antimicrobial resistance genes (ARGs), which significantly clustered by age, with the influence of host genetics on antimicrobial resistance increasing over time. Our results suggest the mobilisation potential of ARGs increased with age as Tn916 transposase was co-located with more species and ARGs as children grew. Dental caries compared to health was associated with a depletion of ARGs and species, which was reversed when restored. Our results highlight the dynamic nature of the paediatric oral resistome, and its potential role in transmission of AMR and dysbiosis.

text: Haemophilus haemolyticus

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pasteurellales;f__Pasteurellaceae;g__Haemophilus;s__Haemophilus haemolyticus

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968832_bin.31_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: Cardiobacterium hominis

characteristic: project name

characteristic: sample derived from

text: Cardiobacterium hominis

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Cardiobacteriales;f__Cardiobacteriaceae;g__Cardiobacterium;s__Cardiobacterium hominis

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968832_bin.39_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: Rothia terrae

characteristic: organism

text: Rothia terrae

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Actinobacteria;c__Actinomycetia;o__Micrococcales;f__Micrococcaceae;g__Rothia;s__Rothia terrae

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968832_bin.41_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: uncultured Prevotella sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Prevotella sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Prevotellaceae;g__Prevotella;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968832_bin.44_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968834 of study ERP139515.

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: uncultured Leptotrichia sp.

characteristic: organism

text: uncultured Leptotrichia sp.

text: Illumina NovaSeq 6000

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968834_bin.10_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968835 of study ERP139515.

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: uncultured Leptotrichiaceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Leptotrichiaceae bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Fusobacteria;c__Fusobacteriia;o__Fusobacteriales;f__Leptotrichiaceae;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968835_bin.18_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: Pseudomonas putida

characteristic: organism

text: Pseudomonas putida

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas;s__Pseudomonas putida

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968835_bin.25_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: uncultured Pantoea sp.

text: Antibiotic overuse has promoted the spread of antimicrobial resistance (AMR) with significant health and economic consequences. Surveillance of resistance reservoirs, like the rarely explored oral microbiome, is necessary to combat AMR. We characterised the development of the paediatric oral resistome and investigated its role in dental caries in 221 twin children sampled over the first decade of life. From 535 oral metagenomes, we identified 309 antimicrobial resistance genes (ARGs), which significantly clustered by age, with the influence of host genetics on antimicrobial resistance increasing over time. Our results suggest the mobilisation potential of ARGs increased with age as Tn916 transposase was co-located with more species and ARGs as children grew. Dental caries compared to health was associated with a depletion of ARGs and species, which was reversed when restored. Our results highlight the dynamic nature of the paediatric oral resistome, and its potential role in transmission of AMR and dysbiosis.

characteristic: project name

characteristic: sample derived from

text: uncultured Pantoea sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Erwiniaceae;g__Pantoea;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968835_bin.8_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968836 of study ERP139515.

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: Actinomyces oris

characteristic: organism

text: Actinomyces oris

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Actinobacteria;c__Actinomycetia;o__Actinomycetales;f__Actinomycetaceae;g__Actinomyces;s__Actinomyces oris

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968836_bin.12_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: uncultured Candidatus Saccharibacteria bacterium

characteristic: project name

characteristic: sample derived from

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Candidatus Saccharibacteria;c__Candidatus Saccharimonia;o__Candidatus Nanosynbacterales;f__Candidatus Nanosynbacteraceae;g__Candidatus Nanosynbacter;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968836_bin.14_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

characteristic: organism

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Candidatus Saccharibacteria;c__;o__;f__;g__;s__

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968836_bin.17_MetaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaspades v3.15.3

text: MetaWRAP v1.3

text: human digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: plaque biofilm

characteristic: environmental medium

text: Australia

text: metagenome-assembled genome

characteristic: investigation type

text: oral metagenome

text: oral cavity

characteristic: local environmental context

text: oral metagenome

characteristic: metagenomic source

text: Streptococcus sanguinis

characteristic: project name

characteristic: sample derived from

text: Streptococcus sanguinis

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Streptococcus;s__Streptococcus sanguinis

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-Assembled Genome: ERR9968836_bin.4_MetaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaspades v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: MetaWRAP v1.3

characteristic: binning software

text: human digestive system

text: EBI-EMG

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968837 of study ERP139515.

characteristic: description

text: plaque biofilm

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: oral metagenome

text: oral cavity

text: oral metagenome

text: Prevotella pallens

characteristic: organism

text: Prevotella pallens

text: Illumina NovaSeq 6000

text: The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Prevotellaceae;g__Prevotella;s__Prevotella pallens

text: multi-marker approach

text: Metagenome-Assembled Genome: ERR9968837_bin.12_MetaWRAP_v1.3_MAG