text: Quadram Institute Bioscience

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: SE

characteristic: SRA accession

characteristic: Site

characteristic: Submitter Id

text: soil metagenome

characteristic: common name

text: soil metagenome

text: terrestrial biome

text: Forest

text: soil

text: Sweden

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: soil metagenome

text: euroSoilMG

characteristic: project name

text: soil metagenome

characteristic: sequencing method

text: soil

characteristic: soil environmental package


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Quadram Institute Bioscience

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: DK

tag: Namespace:Quadram Institute Bioscience

text: soil metagenome

text: soil metagenome

characteristic: description

text: terrestrial biome

characteristic: environment (biome)

text: Forest

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: Denmark

characteristic: geographic location (country and/or sea)

unit: m

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

unit: DD

characteristic: geographic location (latitude)

text: metagenome

text: soil metagenome

characteristic: organism

text: euroSoilMG

text: soil metagenome

text: Illumina NovaSeq with 2 x 150 bp paired-end

text: soil

characteristic: title


text: Quadram Institute Bioscience

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: DE

characteristic: SRA accession

characteristic: Site

characteristic: Submitter Id

text: soil metagenome

characteristic: common name

text: soil metagenome

text: terrestrial biome

text: Forest

text: soil

text: Germany

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: soil metagenome

text: euroSoilMG

characteristic: project name

text: soil metagenome

characteristic: sequencing method

text: soil

characteristic: soil environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Quadram Institute Bioscience

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: IT

text: soil metagenome

text: soil metagenome

characteristic: description

text: terrestrial biome

characteristic: environment (biome)

text: Forest

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: Italy

characteristic: geographic location (country and/or sea)

text: metagenome

text: soil metagenome

text: euroSoilMG

text: soil metagenome

text: soil


text: SYSU

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: Protocols: Transcriptomic analysis was conducted to measure the transcription of these sulfur metabolism genes in DvH cultures amended with two different molar ratios of lactate to sulfate, i.e., 1:1 and 2:1. Briefly, samples for total RNA extraction were collected by centrifugation (5 min, 10,000×g, 4°C) from triplicate cultures at mid-exponential phase (OD600 = ~0.4), and then mixed with TRIzolTM reagent (Invitrogen; Waltham, MA, USA). Total RNA was extracted with a RNeasy Mini kit (Qiagen), from which fragmented DNA was removed using DNase I. The highly transcribed ribosomal RNAs (rRNAs) were eliminated using the RiBoCop rRNA depletion Kit (Lexogen; Vienna, Austria). The RNA-Seq libraries were constructed from the rRNA-depleted RNA samples using NEB Next® Ultra™ Directional RNA Library Prep Kit (New England Biolabs; Ipswich, MA, USA)

text: logarithmic phase culture

characteristic: growth condition

text: not applicable

characteristic: isolate

text: Desulfovibrio vulgaris str. Hildenborough

characteristic: organism

text: lactate:sulfate 1:1

characteristic: stimulus

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SYSU

characteristic: INSDC center name

text: public

text: ArrayExpress

text: logarithmic phase culture

text: not applicable

text: lactate:sulfate 2:1


characteristic: INSDC first public

text: public

tag: Namespace:Surgery Department at University of Texas Southwestern Medical center at Dallas

text: ArrayExpress

text: human

characteristic: common name

text: Protocols: 1)plate Panc-1 cells 6x105/well in 6-wells cell culture plates with DMEM medium containing 10% FBS and 1%Pen/Strep on day1. 2) cells were treated with or without RSL3,N6F11,Fin56, and Erastin for 6hours on day2. 3)Aspirate and discard the cell culture medium, wash the cells with 1XPBS buffer X2 times. 4)collect cell lysates with 35oul GTC Lysis Buffer/well. Vortex or pipet up and down to mix thoroughly. GTC lysis buffer(E.Z.N.A.® HP Total RNA Kit,Cat#R6812-02, Omega) RNA extraction: Using E.Z.N.A.® HP Total RNA Kit,Cat#R6812-02, Omega 1) Insert a RNA Homogenizer Mini Column into a 2 mL Collection Tube. 2)Transfer the lysate to the RNA Homogenizer Mini Column. 3)Centrifuge at 13,000 x g for 1 minute 4)Add 1 volume 70% ethanol. Vortex to mix thoroughly. Do not centrifugeve the filtrate and discard the RNA Homogenizer Mini Column. 5)Insert a HiBind® RNA Mini Column into a 2 mL Collection Tube. 6)Transfer 700 μL sample (including any precipitate that may have formed) to the HiBind® RNA Mini Column. 7)Centrifuge at 10,000 x g for 1 minute 8)Discard the filtrate and reuse the Collection Tube 9)Add 500 μL RNA Wash Buffer II. 10)Centrifuge at 10,000 x g for 1 minute 11)Discard the filtrate and reuse the Collection Tube 12)Centrifuge at maximum speed for 2 minutes to completely dry the HiBind® RNA Mini Column. 13)Transfer the HiBind® RNA Mini Column to a clean 1.5 mL microcentrifuge tube (not provided) 14)Add 40-70 μL Nuclease-free Water. 15)Centrifuge at maximum speed for 2 minutes then measure the RNA concentration and store eluted RNA at -80°C. An Illumina TruSeq Stranded mRNA Library prep kit (20020594) was used to generate the RNA libraries.Illumina TruSeq Stranded mRNA Sample Preparation Protocol. In briefly protocol is described as below: A: on day1  Step 1: Isolate mRNA  Step 2: 1st strand cDNA synthesis  Step 3: 2nd strand cDNA synthesis  Step 4: Reaction clean-up B: on day2  Step 5: Adenylate cDNA ends  Step 6: ligate adapters to ends  Step 7: PCR amplify library  Normalize and Pool  Setup Run in Basespace  Loading the Sequencer

characteristic: description

text: adult

characteristic: developmental stage

text: epithelioid carcinoma

characteristic: disease

text: wild type genotype

characteristic: genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: pancreatic duct

characteristic: organism part

text: Homo sapiens


text: Surgery Department at University of Texas Southwestern Medical center at Dallas

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: human

text: adult

text: epithelioid carcinoma

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: pancreatic duct

text: Homo sapiens


characteristic: ENA-CHECKLIST

text: public

tag: Namespace:Institute for Research on Cancer and Aging, Nice (IRCAN) Inserm U1081 - CNRS UMR 7284

text: ArrayExpress

text: human

text: Protocols: HCT-116 cells were cultured in McCoy medium supplemented with 10% FBS, 100 U/mL penicillin and 100 µg/mL streptomycin. For 5-aza-2'-deoxycytidine (DAC) treatment, cells were plated at a density of 100,000 cells/well in 6-well plates and treated with DAC at a final concentration of 1 μM for a total of 5 days. Fresh medium and drug were added daily for the first 3 days. In control samples (mock), 5-aza was replaced by DMSO. Genomic DNA was extracted and prepared using QiaAmp DNA Blood mini kit (Qiagen) In brief, bs-ATLAS-seq follows these steps: fragmentation of genomic DNA by sonication, ligation of a methylated adapter, bisulfite treatment of linker-ligated DNA, suppression PCR-amplification of L1 5' junctions, and asymmetric Illumina paired-end sequencing (90 bp + 210 bp). Read #1 starts from a random position (sonicated DNA break), while read #2 starts from a fixed sequence in L1 5' UTR (internal promoter). With this design, read #2 covers the 15 most 5’ CpG sites (1-207, relative to L1HS consensus sequence). Read #1 has the same orientation as the L1 element.

text: colorectal carcinoma

text: wild type genotype

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: mixed large intestine and colon

text: Homo sapiens

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Institute for Research on Cancer and Aging, Nice (IRCAN) Inserm U1081 - CNRS UMR 7284

characteristic: INSDC center name

text: public

text: ArrayExpress

text: human

text: colorectal carcinoma

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mixed large intestine and colon

text: Homo sapiens


characteristic: INSDC first public

text: public

text: ArrayExpress

text: colorectal cancer cell

text: human

characteristic: common name

text: Protocols: HCT-116 cells were cultured in McCoy medium supplemented with 10% FBS, 100 U/mL penicillin and 100 µg/mL streptomycin. For 5-aza-deoxycytidine (DAC) treatment, cells were plated at a density of 100,000 cells/well in 6-well plates and treated with DAC at a final concentration of 1 μM for a total of 5 days. Fresh medium and drug were added daily for the first 3 days. Total RNA was purified by two successive cycles of TRI Reagent extraction Directional poly(A)+ RNA-Seq libraries were prepared using NEBNext Ultra II Directional RNA Library Prep kit for Illumina

characteristic: description

text: colorectal adenocarcinoma

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: male

characteristic: sex


characteristic: INSDC last update

text: public

text: ArrayExpress

text: breast adenocarcinoma cell

text: human

text: Protocols: Cells were grown and collected from culture dishes. Total RNA was purified by two successive cycles of TRI Reagent extraction Directional poly(A)+ RNA-Seq libraries were prepared using NEBNext Ultra II Directional RNA Library Prep kit for Illumina

text: adult

characteristic: developmental stage

text: breast adenocarcinoma

characteristic: disease

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

characteristic: organism part

text: Homo sapiens

text: female


text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: cervical adenocarcinoma cell

text: human

text: adult

text: cervical adenocarcinoma

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: ovary

text: Homo sapiens

text: female


characteristic: ENA-CHECKLIST

text: public

text: ArrayExpress

text: chronic myelogenous leukemia cell

text: human

text: adult

text: chronic myelogenous leukemia

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: Homo sapiens

text: female

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: ArrayExpress

text: colorectal cancer cell

text: human

text: adult

text: colorectal adenocarcinoma

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: male


characteristic: INSDC first public

text: public

text: ArrayExpress

text: embryonal carcinoma cell

text: human

characteristic: common name

characteristic: description

text: adult

text: embryonal carcinoma

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: testis

text: Homo sapiens

text: male

characteristic: sex


characteristic: INSDC last update

text: public

text: ArrayExpress

text: hepatocellular carcinoma cell

text: human

text: juvenile stage

characteristic: developmental stage

text: hepatocellular carcinoma

characteristic: disease

text: not applicable

text: Homo sapiens

text: Homo sapiens

characteristic: organism part

text: Homo sapiens

text: male


text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: BJ

text: fibroblast of dermis

text: human

text: neonate

text: normal

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin of prepuce of penis

text: Homo sapiens

text: male

text: BJ


characteristic: ENA-CHECKLIST

text: public

text: ArrayExpress

text: immortalized embryonic kidney cell

text: human

text: embryo stage

text: normal

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: kidney

text: Homo sapiens

text: female

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: ArrayExpress

text: immortalized embryonic kidney cell (transformed with SV40 Large T)

text: human

text: embryo stage

text: normal

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: kidney

text: Homo sapiens

text: female


characteristic: INSDC first public

text: public

text: ArrayExpress

text: fibroblast of lung

text: human

characteristic: common name

characteristic: description

text: late embryonic stage

text: normal

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: lung

text: Homo sapiens

text: female

characteristic: sex


characteristic: INSDC last update

text: public

text: ArrayExpress

text: fibroblast of lung

text: human

text: late embryonic stage

characteristic: developmental stage

text: normal

characteristic: disease

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: lung

characteristic: organism part

text: Homo sapiens

text: male


text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: embryonal carcinoma cell

text: human

text: Protocols: Cells were grown and collected from culture dishes. Genomic DNA was extracted using the Monarch Genomic DNA Purification kit. 125 sgRNAs targeting the region downstream of L1HS elements (one unique sgRNA per locus) were synthesized using the EnGen sgRNA Synthesis kit (New England Biolabs). Targeted librairy preparation follows these steps: assembly of Cas9 ribonucleoprotein particles (RNP), dephosphorylation of genomic DNA, incubation of RNP assemble with genomic DNA, library preparation and Oxford Nanopore sequencing. With this design, sequencing starts at the sgRNA binding site in the region downstream of L1 and progresses towards the target L1 in the antisense direction

text: adult

text: embryonal carcinoma

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: testis

text: Homo sapiens

text: male


characteristic: ENA-CHECKLIST

text: public

text: ArrayExpress

text: colorectal adenocarcinoma cell

text: human

text: adult

text: colorectal adenocarcinoma

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: colon

text: Homo sapiens

text: male

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: ArrayExpress

text: chronic myelogenous leukemia cell

text: human

text: adult

text: chronic myelogenous leukemia

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: Homo sapiens

text: female


characteristic: INSDC first public

text: public

text: ArrayExpress

text: breast adenocarcinoma cell

text: human

characteristic: common name

characteristic: description

text: adult

text: breast adenocarcinoma

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: female

characteristic: sex


E-MTAB-12249:H3K4me3 ChIP

text: Universite Cote d'Azur, Inserm, CNRS

characteristic: INSDC last update

text: public

text: E-MTAB-12249:H3K4me3 ChIP

tag: Namespace:Universite Cote d'Azur, Inserm, CNRS

text: ArrayExpress

text: human

text: Protocols: ChIP for H3K4me3 (Abcam ab8580) and YY1 (Diagenode, C15410345) were performed using sonicated extracts from formaldehyde-fixed nuclei. DNA was extracted from immunoprecipitated or input chromatin using Qiagen MinElute kit. Sequencing libraries were prepared using the NebNext Ultra II DNA library kit.

text: adult

characteristic: developmental stage

text: embryonal carcinoma

characteristic: disease

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: testis

characteristic: organism part

text: Homo sapiens

text: H3K4me3 ChIP


E-MTAB-12249:Input DNA

text: Universite Cote d'Azur, Inserm, CNRS

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12249:Input DNA

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: human

text: adult

text: embryonal carcinoma

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: testis

text: Homo sapiens

text: Input DNA


E-MTAB-12249:YY1 ChIP

characteristic: ENA-CHECKLIST

text: Universite Cote d'Azur, Inserm, CNRS

text: public

text: E-MTAB-12249:YY1 ChIP

text: ArrayExpress

text: human

text: adult

text: embryonal carcinoma

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: testis

text: Homo sapiens

text: YY1 ChIP

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Rostock

characteristic: INSDC center name

text: public

text: 69552:460deefa-7374-4f04-8455-485a85e05aeb

tag: Namespace:University Rostock

text: GFBIO

text: Biological soil crust_Moss-dominated, Warnemuende, coastal dune

text: terrestrial biome [ENVO:00000446]|temperate biome [ENVO:01001831]

characteristic: environment (biome)

text: dune [ENVO:00000170]|coastal dune [ENVO:00000416]|organic object formed through microbial activity [ENVO:01000007]

characteristic: environment (feature)

text: organic material [ENVO:01000155]|microbial mat material [ENVO:01000157]

characteristic: environment (material)

text: microbial mat/biofilm

characteristic: environmental package

text: Yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Mecklenburg-Western Pommerania, Warnemuende

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: soil crust metagenome

text: FWD:CCTACGGGNGGCWGCAG REV:GACTACHVGGGTATCTAAKCC

characteristic: pcr primers

text: Microbial community composition of biological soil crusts in coastal sand dunes in northern Germany

characteristic: project name

characteristic: replicate

text: soil crust metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Coastal protection dune

characteristic: site

text: true

characteristic: submitted to insdc

characteristic: target subfragment


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Rostock

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 69551:460deefa-7374-4f04-8455-485a85e05aeb

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: description

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: microbial mat/biofilm

text: Yes

text: Germany

text: mimarks-survey

text: microbial mat/biofilm

text: soil crust metagenome

characteristic: organism

text: soil crust metagenome

text: Illumina MiSeq

text: Coastal protection dune

text: true

characteristic: title


characteristic: ENA-CHECKLIST

text: University Rostock

text: public

text: 69550:460deefa-7374-4f04-8455-485a85e05aeb

text: GFBIO

text: microbial mat/biofilm

characteristic: environmental package

text: Yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: soil crust metagenome

characteristic: pcr primers

characteristic: project name

characteristic: replicate

text: soil crust metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Coastal protection dune

characteristic: site

text: true

characteristic: submitted to insdc

characteristic: target subfragment


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Rostock

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 69549:460deefa-7374-4f04-8455-485a85e05aeb

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

text: Biological soil crust_Moss-dominated, Prora (Ruegen), grey dune

characteristic: description

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: microbial mat/biofilm

text: Yes

text: Germany

text: Mecklenburg-Western Pommerania, Prora

text: mimarks-survey

text: microbial mat/biofilm

text: soil crust metagenome

characteristic: organism

text: soil crust metagenome

text: Illumina MiSeq

text: Grey dune area

text: true

characteristic: title


characteristic: ENA-CHECKLIST

text: University Rostock

text: public

text: 69548:460deefa-7374-4f04-8455-485a85e05aeb

text: GFBIO

text: microbial mat/biofilm

characteristic: environmental package

text: Yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Mecklenburg-Western Pommerania, Prora

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: soil crust metagenome

characteristic: pcr primers

characteristic: project name

characteristic: replicate

text: soil crust metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Grey dune area

characteristic: site

text: true

characteristic: submitted to insdc

characteristic: target subfragment


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Rostock

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 69547:460deefa-7374-4f04-8455-485a85e05aeb

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: description

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: microbial mat/biofilm

text: Yes

text: Germany

text: Mecklenburg-Western Pommerania, Prora

text: mimarks-survey

text: microbial mat/biofilm

text: soil crust metagenome

characteristic: organism

text: soil crust metagenome

text: Illumina MiSeq

text: Grey dune area

text: true

characteristic: title


characteristic: ENA-CHECKLIST

text: University Rostock

text: public

text: 69546:460deefa-7374-4f04-8455-485a85e05aeb

text: GFBIO

text: Biological soil crust_Moss-dominated, Prora (Ruegen), dune edge

text: microbial mat/biofilm

characteristic: environmental package

text: Yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Mecklenburg-Western Pommerania, Prora

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: soil crust metagenome

characteristic: pcr primers

characteristic: project name

characteristic: replicate

text: soil crust metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Dune edge close to shore

characteristic: site

text: true

characteristic: submitted to insdc

characteristic: target subfragment


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Rostock

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 69545:460deefa-7374-4f04-8455-485a85e05aeb

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: description

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: microbial mat/biofilm

text: Yes

text: Germany

text: Mecklenburg-Western Pommerania, Prora

text: mimarks-survey

text: microbial mat/biofilm

text: soil crust metagenome

characteristic: organism

text: soil crust metagenome

text: Illumina MiSeq

text: Dune edge close to shore

text: true

characteristic: title


characteristic: ENA-CHECKLIST

text: University Rostock

text: public

text: 69544:460deefa-7374-4f04-8455-485a85e05aeb

text: GFBIO

text: microbial mat/biofilm

characteristic: environmental package

text: Yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Mecklenburg-Western Pommerania, Prora

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: soil crust metagenome

characteristic: pcr primers

characteristic: project name

characteristic: replicate

text: soil crust metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Dune edge close to shore

characteristic: site

text: true

characteristic: submitted to insdc

characteristic: target subfragment


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Rostock

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 69543:460deefa-7374-4f04-8455-485a85e05aeb

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

text: Biological soil crust_Moss-dominated, Coastal protection dune Prerow, inland slope

characteristic: description

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: microbial mat/biofilm

text: Yes

text: Germany

text: Mecklenburg-Western Pommerania, Prerow, Coastal protection dune

text: mimarks-survey

text: microbial mat/biofilm

text: soil crust metagenome

characteristic: organism

text: soil crust metagenome

text: Illumina MiSeq

text: Coastal protection dune. Inland dune slope

text: true

characteristic: title


characteristic: ENA-CHECKLIST

text: University Rostock

text: public

text: 69542:460deefa-7374-4f04-8455-485a85e05aeb

text: GFBIO

text: terrestrial biome [ENVO:00000446]|temperate biome [ENVO:01001831]

text: dune [ENVO:00000170]|coastal dune [ENVO:00000416]|organic object formed through microbial activity [ENVO:01000007]

text: organic material [ENVO:01000155]|microbial mat material [ENVO:01000157]

text: microbial mat/biofilm

characteristic: environmental package

text: Yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: soil crust metagenome

characteristic: pcr primers

characteristic: project name

characteristic: replicate

text: soil crust metagenome

text: Illumina MiSeq

characteristic: sequencing method

characteristic: site

text: true

characteristic: submitted to insdc

characteristic: target subfragment


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Rostock

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 69541:460deefa-7374-4f04-8455-485a85e05aeb

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: description

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: microbial mat/biofilm

text: Yes

text: Germany

text: mimarks-survey

text: microbial mat/biofilm

text: soil crust metagenome

characteristic: organism

text: FWD:CCTACGGGNGGCWGCAG REV:GACTACHVGGGTATCTAAKCC

text: Microbial community composition of biological soil crusts in coastal sand dunes in northern Germany

text: soil crust metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA-CHECKLIST

text: University Rostock

text: public

text: 69540:460deefa-7374-4f04-8455-485a85e05aeb

text: GFBIO

text: Biological soil crust_Moss-dominated, Coastal protection dune Prerow, dune crest inland

text: microbial mat/biofilm

characteristic: environmental package

text: Yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: soil crust metagenome

characteristic: pcr primers

characteristic: project name

characteristic: replicate

text: soil crust metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Coastal protection dune. Dune crest toward the inland dune slope

characteristic: site

text: true

characteristic: submitted to insdc

characteristic: target subfragment


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Rostock

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 69539:460deefa-7374-4f04-8455-485a85e05aeb

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: description

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: microbial mat/biofilm

text: Yes

text: Germany

text: mimarks-survey

text: microbial mat/biofilm

text: soil crust metagenome

characteristic: organism

text: soil crust metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA-CHECKLIST

text: University Rostock

text: public

text: 69538:460deefa-7374-4f04-8455-485a85e05aeb

text: GFBIO

text: microbial mat/biofilm

characteristic: environmental package

text: Yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: soil crust metagenome

characteristic: pcr primers

characteristic: project name

characteristic: replicate

text: soil crust metagenome

text: Illumina MiSeq

characteristic: sequencing method

characteristic: site

text: true

characteristic: submitted to insdc

characteristic: target subfragment


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Rostock

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 69537:460deefa-7374-4f04-8455-485a85e05aeb

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

text: Biological soil crust_Moss-dominated, Pramort, mature dune

characteristic: description

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: microbial mat/biofilm

text: Yes

text: Germany

text: Mecklenburg-Western Pommerania, Zingst

text: mimarks-survey

text: microbial mat/biofilm

text: soil crust metagenome

characteristic: organism

text: soil crust metagenome

text: Illumina MiSeq

text: Mature dune area, overgrown by pine trees

text: true

characteristic: title


characteristic: ENA-CHECKLIST

text: University Rostock

text: public

text: 69536:460deefa-7374-4f04-8455-485a85e05aeb

text: GFBIO

text: microbial mat/biofilm

characteristic: environmental package

text: Yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Mecklenburg-Western Pommerania, Zingst

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: soil crust metagenome

characteristic: pcr primers

characteristic: project name

characteristic: replicate

text: soil crust metagenome

text: Illumina MiSeq

characteristic: sequencing method

characteristic: site

text: true

characteristic: submitted to insdc

characteristic: target subfragment


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Rostock

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 69535:460deefa-7374-4f04-8455-485a85e05aeb

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: description

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: microbial mat/biofilm

text: Yes

text: Germany

text: Mecklenburg-Western Pommerania, Zingst

text: mimarks-survey

text: microbial mat/biofilm

text: soil crust metagenome

characteristic: organism

text: soil crust metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA-CHECKLIST

text: University Rostock

text: public

text: 69534:460deefa-7374-4f04-8455-485a85e05aeb

text: GFBIO

text: Biological soil crust_Moss-dominated, Pramort, grey dune

text: microbial mat/biofilm

characteristic: environmental package

text: Yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Mecklenburg-Western Pommerania, Zingst

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: soil crust metagenome

characteristic: pcr primers

characteristic: project name

characteristic: replicate

text: soil crust metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Grey dune area

characteristic: site

text: true

characteristic: submitted to insdc

characteristic: target subfragment


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Rostock

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 69533:460deefa-7374-4f04-8455-485a85e05aeb

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: description

text: terrestrial biome [ENVO:00000446]|temperate biome [ENVO:01001831]

characteristic: environment (biome)

text: dune [ENVO:00000170]|coastal dune [ENVO:00000416]|organic object formed through microbial activity [ENVO:01000007]

characteristic: environment (feature)

characteristic: environment (material)

text: microbial mat/biofilm

text: Yes

text: Germany

text: Mecklenburg-Western Pommerania, Zingst

text: mimarks-survey

text: microbial mat/biofilm

text: soil crust metagenome

characteristic: organism

text: soil crust metagenome

text: Illumina MiSeq

text: Grey dune area

text: true

characteristic: title


characteristic: ENA-CHECKLIST

text: University Rostock

text: public

text: 69532:460deefa-7374-4f04-8455-485a85e05aeb

text: GFBIO

text: organic material [ENVO:01000155]|microbial mat material [ENVO:01000157]

text: microbial mat/biofilm

characteristic: environmental package

text: Yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Mecklenburg-Western Pommerania, Zingst

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: soil crust metagenome

text: FWD:CCTACGGGNGGCWGCAG REV:GACTACHVGGGTATCTAAKCC

characteristic: pcr primers

characteristic: project name

characteristic: replicate

text: soil crust metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Grey dune area

characteristic: site

text: true

characteristic: submitted to insdc

characteristic: target subfragment


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Rostock

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 69531:460deefa-7374-4f04-8455-485a85e05aeb

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

text: Biological soil crust_Green algae-dominated, Pramort, white dune

characteristic: description

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: microbial mat/biofilm

text: Yes

text: Germany

text: Mecklenburg-Western Pommerania, Zingst

text: mimarks-survey

text: microbial mat/biofilm

text: soil crust metagenome

characteristic: organism

text: Microbial community composition of biological soil crusts in coastal sand dunes in northern Germany

text: soil crust metagenome

text: Illumina MiSeq

text: Yellow dune area

text: true

characteristic: title


characteristic: ENA-CHECKLIST

text: University Rostock

text: public

text: 69530:460deefa-7374-4f04-8455-485a85e05aeb

text: GFBIO

text: microbial mat/biofilm

characteristic: environmental package

text: Yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Mecklenburg-Western Pommerania, Zingst

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: soil crust metagenome

characteristic: pcr primers

characteristic: project name

characteristic: replicate

text: soil crust metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Yellow dune area

characteristic: site

text: true

characteristic: submitted to insdc

characteristic: target subfragment


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Rostock

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 69529:460deefa-7374-4f04-8455-485a85e05aeb

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: description

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: microbial mat/biofilm

text: Yes

text: Germany

text: Mecklenburg-Western Pommerania, Zingst

text: mimarks-survey

text: microbial mat/biofilm

text: soil crust metagenome

characteristic: organism

text: soil crust metagenome

text: Illumina MiSeq

text: Yellow dune area

text: true

characteristic: title


characteristic: ENA-CHECKLIST

text: University Rostock

text: public

text: 69528:460deefa-7374-4f04-8455-485a85e05aeb

characteristic: broad-scale environmental context

text: GFBIO

characteristic: collection date

characteristic: depth

text: Bare sand_occasionally submerged, Pramort, windwatt

characteristic: elevation

characteristic: environmental medium

text: sediment

characteristic: environmental package

text: yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Mecklenburg-Western Pommerania, Zingst

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: dune [ENVO:00000170]|coastal dune [ENVO:00000416]

characteristic: local environmental context

text: beach sand metagenome

characteristic: pcr primers

characteristic: project name

characteristic: replicate

text: beach sand metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina MiSeq

characteristic: sequencing method

text: Windwatt, mudflat

characteristic: site

text: true

characteristic: submitted to insdc

characteristic: target subfragment

text: POWW


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Rostock

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 69527:460deefa-7374-4f04-8455-485a85e05aeb

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

text: Biological soil crust_Moss-dominated, Dar������er

characteristic: description

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: microbial mat/biofilm

text: Yes

text: Germany

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

text: Mecklenburg-Western Pommerania, Darß peninsul

text: mimarks-survey

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: soil crust metagenome

characteristic: organism

text: soil crust metagenome

text: Illumina MiSeq

text: Mature dune area, overgrown by pine trees

text: true

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Rostock

text: public

text: 69526:460deefa-7374-4f04-8455-485a85e05aeb

text: GFBIO

text: terrestrial biome [ENVO:00000446]|temperate biome [ENVO:01001831]

text: microbial mat/biofilm

characteristic: environmental package

text: Yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

text: soil crust metagenome

characteristic: pcr primers

characteristic: project name

characteristic: replicate

text: soil crust metagenome

text: Illumina MiSeq

characteristic: sequencing method

characteristic: site

text: true

characteristic: submitted to insdc

characteristic: target subfragment


characteristic: ENA-CHECKLIST

text: University Rostock

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 69525:460deefa-7374-4f04-8455-485a85e05aeb

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: description

characteristic: environment (biome)

text: dune [ENVO:00000170]|coastal dune [ENVO:00000416]|organic object formed through microbial activity [ENVO:01000007]

characteristic: environment (feature)

characteristic: environment (material)

text: microbial mat/biofilm

text: Yes

text: Germany

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

text: mimarks-survey

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: soil crust metagenome

characteristic: organism

text: soil crust metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Rostock

text: public

text: 69524:460deefa-7374-4f04-8455-485a85e05aeb

text: GFBIO

text: Biological soil crust_Moss-dominated, Dar������er Ort

text: organic material [ENVO:01000155]|microbial mat material [ENVO:01000157]

text: microbial mat/biofilm

characteristic: environmental package

text: Yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

text: soil crust metagenome

text: FWD:CCTACGGGNGGCWGCAG REV:GACTACHVGGGTATCTAAKCC

characteristic: pcr primers

characteristic: project name

characteristic: replicate

text: soil crust metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Late grey dune, older grey dune area with dense cryptogamic cover

characteristic: site

text: true

characteristic: submitted to insdc

characteristic: target subfragment


characteristic: ENA-CHECKLIST

text: University Rostock

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 69523:460deefa-7374-4f04-8455-485a85e05aeb

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: description

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: microbial mat/biofilm

text: Yes

text: Germany

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

text: mimarks-survey

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: soil crust metagenome

characteristic: organism

text: Microbial community composition of biological soil crusts in coastal sand dunes in northern Germany

text: soil crust metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Rostock

text: public

text: 69522:460deefa-7374-4f04-8455-485a85e05aeb

text: GFBIO

text: microbial mat/biofilm

characteristic: environmental package

text: Yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

text: soil crust metagenome

characteristic: pcr primers

characteristic: project name

characteristic: replicate

text: soil crust metagenome

text: Illumina MiSeq

characteristic: sequencing method

characteristic: site

text: true

characteristic: submitted to insdc

characteristic: target subfragment


characteristic: ENA-CHECKLIST

text: University Rostock

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 69521:460deefa-7374-4f04-8455-485a85e05aeb

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

text: Biological soil crust_Moss-dominated, Dar������er Ort,

characteristic: description

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: microbial mat/biofilm

text: Yes

text: Germany

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

text: mimarks-survey

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: soil crust metagenome

characteristic: organism

text: soil crust metagenome

text: Illumina MiSeq

text: Early grey dune, young grey dune area still highly influenced by wind

text: true

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Rostock

text: public

text: 69520:460deefa-7374-4f04-8455-485a85e05aeb

text: GFBIO

text: microbial mat/biofilm

characteristic: environmental package

text: Yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Mecklenburg-Western Pommerania, Darß peninsul

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

text: soil crust metagenome

characteristic: pcr primers

characteristic: project name

characteristic: replicate

text: soil crust metagenome

text: Illumina MiSeq

characteristic: sequencing method

characteristic: site

text: true

characteristic: submitted to insdc

characteristic: target subfragment


characteristic: ENA-CHECKLIST

text: University Rostock

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 69519:460deefa-7374-4f04-8455-485a85e05aeb

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: description

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: microbial mat/biofilm

text: Yes

text: Germany

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

text: mimarks-survey

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: soil crust metagenome

characteristic: organism

text: soil crust metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Rostock

text: public

text: 69518:460deefa-7374-4f04-8455-485a85e05aeb

text: GFBIO

text: Biological soil crust_Green algae-dominated, Dar������er

text: terrestrial biome [ENVO:00000446]|temperate biome [ENVO:01001831]

text: microbial mat/biofilm

characteristic: environmental package

text: Yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

text: soil crust metagenome

characteristic: pcr primers

characteristic: project name

characteristic: replicate

text: soil crust metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Yellow dune area

characteristic: site

text: true

characteristic: submitted to insdc

characteristic: target subfragment


characteristic: ENA-CHECKLIST

text: University Rostock

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 69517:460deefa-7374-4f04-8455-485a85e05aeb

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: description

characteristic: environment (biome)

text: dune [ENVO:00000170]|coastal dune [ENVO:00000416]|organic object formed through microbial activity [ENVO:01000007]

characteristic: environment (feature)

characteristic: environment (material)

text: microbial mat/biofilm

text: Yes

text: Germany

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

text: mimarks-survey

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: soil crust metagenome

characteristic: organism

text: soil crust metagenome

text: Illumina MiSeq

text: Yellow dune area

text: true

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Rostock

text: public

text: 69516:460deefa-7374-4f04-8455-485a85e05aeb

text: GFBIO

text: organic material [ENVO:01000155]|microbial mat material [ENVO:01000157]

text: microbial mat/biofilm

characteristic: environmental package

text: Yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

text: soil crust metagenome

characteristic: pcr primers

characteristic: project name

characteristic: replicate

text: soil crust metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Yellow dune area

characteristic: site

text: true

characteristic: submitted to insdc

characteristic: target subfragment


characteristic: ENA-CHECKLIST

text: University Rostock

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 69515:460deefa-7374-4f04-8455-485a85e05aeb

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

text: Biological soil crust_Moss-dominated, Inland dune Verden, close to forest

characteristic: description

characteristic: environment (biome)

text: dune [ENVO:00000170]|organic object formed through microbial activity [ENVO:01000007]

characteristic: environment (feature)

characteristic: environment (material)

text: microbial mat/biofilm

text: Yes

text: Germany

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

text: Lower-Saxony, Verden (Aller)

text: mimarks-survey

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: soil crust metagenome

characteristic: organism

text: FWD:CCTACGGGNGGCWGCAG REV:GACTACHVGGGTATCTAAKCC

text: soil crust metagenome

text: Illumina MiSeq

text: Dune crest: the transect ended in a mixed dune forest area dominated by pine trees

text: true

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Rostock

text: public

text: 69514:460deefa-7374-4f04-8455-485a85e05aeb

text: GFBIO

text: microbial mat/biofilm

characteristic: environmental package

text: Yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Lower-Saxony, Verden (Aller)

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

text: soil crust metagenome

characteristic: pcr primers

text: Microbial community composition of biological soil crusts in coastal sand dunes in northern Germany

characteristic: project name

characteristic: replicate

text: soil crust metagenome

text: Illumina MiSeq

characteristic: sequencing method

characteristic: site

text: true

characteristic: submitted to insdc

characteristic: target subfragment


characteristic: ENA-CHECKLIST

text: University Rostock

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 69513:460deefa-7374-4f04-8455-485a85e05aeb

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: description

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: microbial mat/biofilm

text: Yes

text: Germany

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

text: Lower-Saxony, Verden (Aller)

text: mimarks-survey

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: soil crust metagenome

characteristic: organism

text: soil crust metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Rostock

text: public

text: 69512:460deefa-7374-4f04-8455-485a85e05aeb

text: GFBIO

text: Biological soil crust_Moss-dominated, Inland dune Verden, transitional zone

text: microbial mat/biofilm

characteristic: environmental package

text: Yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Lower-Saxony, Verden (Aller)

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

text: soil crust metagenome

characteristic: pcr primers

characteristic: project name

characteristic: replicate

text: soil crust metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Transitional zone: dune slope (DS) where changes in vegetation cover were obvious, finally reaching to the crestline of the parabolic dune

characteristic: site

text: true

characteristic: submitted to insdc

characteristic: target subfragment


characteristic: ENA-CHECKLIST

text: University Rostock

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 69511:460deefa-7374-4f04-8455-485a85e05aeb

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: description

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: microbial mat/biofilm

text: Yes

text: Germany

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

text: Lower-Saxony, Verden (Aller)

text: mimarks-survey

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: soil crust metagenome

characteristic: organism

text: soil crust metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Rostock

text: public

text: 69510:460deefa-7374-4f04-8455-485a85e05aeb

text: GFBIO

text: terrestrial biome [ENVO:00000446]|temperate biome [ENVO:01001831]

text: microbial mat/biofilm

characteristic: environmental package

text: Yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Lower-Saxony, Verden (Aller)

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

text: soil crust metagenome

characteristic: pcr primers

characteristic: project name

characteristic: replicate

text: soil crust metagenome

text: Illumina MiSeq

characteristic: sequencing method

characteristic: site

text: true

characteristic: submitted to insdc

characteristic: target subfragment


characteristic: ENA-CHECKLIST

text: University Rostock

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 69509:460deefa-7374-4f04-8455-485a85e05aeb

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

text: Biological soil crust_Moss-dominated, Coastal spit Schaabe, mature dune

characteristic: description

characteristic: environment (biome)

text: dune [ENVO:00000170]|coastal dune [ENVO:00000416]|organic object formed through microbial activity [ENVO:01000007]

characteristic: environment (feature)

characteristic: environment (material)

text: microbial mat/biofilm

text: Yes

text: Germany

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

text: Mecklenburg-Western Pomerania, Ruegen, Schaabe spit

text: mimarks-survey

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: soil crust metagenome

characteristic: organism

text: soil crust metagenome

text: Illumina MiSeq

text: Mature dune area, overgrown by pine trees

text: true

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Rostock

text: public

text: 69508:460deefa-7374-4f04-8455-485a85e05aeb

text: GFBIO

text: organic material [ENVO:01000155]|microbial mat material [ENVO:01000157]

text: microbial mat/biofilm

characteristic: environmental package

text: Yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

text: soil crust metagenome

characteristic: pcr primers

characteristic: project name

characteristic: replicate

text: soil crust metagenome

text: Illumina MiSeq

characteristic: sequencing method

characteristic: site

text: true

characteristic: submitted to insdc

characteristic: target subfragment


characteristic: ENA-CHECKLIST

text: University Rostock

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 69507:460deefa-7374-4f04-8455-485a85e05aeb

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: description

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: microbial mat/biofilm

text: Yes

text: Germany

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

text: mimarks-survey

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: soil crust metagenome

characteristic: organism

text: FWD:CCTACGGGNGGCWGCAG REV:GACTACHVGGGTATCTAAKCC

text: soil crust metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Rostock

text: public

text: 69506:460deefa-7374-4f04-8455-485a85e05aeb

text: GFBIO

text: Biological soil crust_Moss-dominated, Coastal spit Schaabe, grey dune

text: microbial mat/biofilm

characteristic: environmental package

text: Yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

text: soil crust metagenome

characteristic: pcr primers

text: Microbial community composition of biological soil crusts in coastal sand dunes in northern Germany

characteristic: project name

characteristic: replicate

text: soil crust metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Grey dune area

characteristic: site

text: true

characteristic: submitted to insdc

characteristic: target subfragment


characteristic: ENA-CHECKLIST

text: University Rostock

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 69505:460deefa-7374-4f04-8455-485a85e05aeb

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: description

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: microbial mat/biofilm

text: Yes

text: Germany

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

text: mimarks-survey

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: soil crust metagenome

characteristic: organism

text: soil crust metagenome

text: Illumina MiSeq

text: Grey dune area

text: true

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Rostock

text: public

text: 69504:460deefa-7374-4f04-8455-485a85e05aeb

text: GFBIO

text: microbial mat/biofilm

characteristic: environmental package

text: Yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

text: soil crust metagenome

characteristic: pcr primers

characteristic: project name

characteristic: replicate

text: soil crust metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Grey dune area

characteristic: site

text: true

characteristic: submitted to insdc

characteristic: target subfragment


characteristic: ENA-CHECKLIST

text: University Rostock

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 69503:460deefa-7374-4f04-8455-485a85e05aeb

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

text: Biological soil crust_Green algae-dominated, Coastal spit Schaabe, white dune

characteristic: description

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: microbial mat/biofilm

text: Yes

text: Germany

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

text: mimarks-survey

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: soil crust metagenome

characteristic: organism

text: soil crust metagenome

text: Illumina MiSeq

text: Yellow dune area

text: true

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Rostock

text: public

text: 69502:460deefa-7374-4f04-8455-485a85e05aeb

text: GFBIO

text: terrestrial biome [ENVO:00000446]|temperate biome [ENVO:01001831]

text: microbial mat/biofilm

characteristic: environmental package

text: Yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

text: soil crust metagenome

characteristic: pcr primers

characteristic: project name

characteristic: replicate

text: soil crust metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Yellow dune area

characteristic: site

text: true

characteristic: submitted to insdc

characteristic: target subfragment


characteristic: ENA-CHECKLIST

text: University Rostock

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 69501:460deefa-7374-4f04-8455-485a85e05aeb

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: description

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: microbial mat/biofilm

text: Yes

text: Germany

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

text: mimarks-survey

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: soil crust metagenome

characteristic: organism

text: soil crust metagenome

text: Illumina MiSeq

text: Yellow dune area

text: true

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Rostock

text: public

text: 69500:460deefa-7374-4f04-8455-485a85e05aeb

characteristic: broad-scale environmental context

text: GFBIO

characteristic: collection date

characteristic: depth

text: Bare sand, Coastal spit Schaabe, primary dune

characteristic: elevation

text: sandy sediment [ENVO:01000118]

characteristic: environmental medium

text: sediment

characteristic: environmental package

text: yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: dune [ENVO:00000170]|coastal dune [ENVO:00000416]

characteristic: local environmental context

text: beach sand metagenome

characteristic: pcr primers

characteristic: project name

characteristic: replicate

text: beach sand metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina MiSeq

characteristic: sequencing method

text: Primary dune area, close to shore

characteristic: site

text: true

characteristic: submitted to insdc

characteristic: target subfragment


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Rostock

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 69499:460deefa-7374-4f04-8455-485a85e05aeb

characteristic: Submitter Id

characteristic: broad-scale environmental context

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: elevation

text: sandy sediment [ENVO:01000118]

text: sediment

text: yes

text: Germany

text: Mecklenburg-Western Pomerania, Ruegen, Schaabe spit

text: mimarks-survey

text: beach sand metagenome

characteristic: organism

text: beach sand metagenome

text: sediment

text: Illumina MiSeq

text: Primary dune area, close to shore

text: true

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: University Rostock

text: public

text: 69498:460deefa-7374-4f04-8455-485a85e05aeb

text: GFBIO

text: sandy sediment [ENVO:01000118]

characteristic: environmental medium

text: sediment

characteristic: environmental package

text: yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

characteristic: local environmental context

text: beach sand metagenome

characteristic: pcr primers

characteristic: project name

characteristic: replicate

text: beach sand metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina MiSeq

characteristic: sequencing method

text: Primary dune area, close to shore

characteristic: site

text: true

characteristic: submitted to insdc

characteristic: target subfragment


text: Streptococcus pneumoniae

characteristic: ArrayExpress-SPECIES

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Streptococcus pneumoniae

characteristic: common name

text: Streptococcus pneumoniae

characteristic: organism

text: Streptococcus pneumoniae

characteristic: title


text: University of Basel

text: public

tag: Namespace:University of Basel

text: soil metagenome samples

characteristic: description

text: soil metagenome

text: soil metagenome


text: mixed

characteristic: ArrayExpress-SEX

text: Caenorhabditis auriculariae

text: Mixed

characteristic: GENDER

text: SC

text: public

text: Caenorhabditis auriculariae

text: Caenorhabditis auriculariae

text: Caenorhabditis auriculariae


text: Stazione Zoologica Anton Dohrn

text: public

tag: Namespace:Stazione Zoologica Anton Dohrn

text: marine biome

characteristic: broad-scale environmental context

characteristic: collection date

text: surface water

characteristic: environmental medium

text: Yes

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: temperate mediterranean sea biome

characteristic: local environmental context

text: marine plankton metagenome

text: CCAGCASCYGCGGTAATTCC ACTTTCGTTCTTGATYRATGA

characteristic: pcr primers

text: marine plankton metagenome

text: 18S SSU rRNA

characteristic: target gene

characteristic: target subfragment


E-MTAB-12288:Sample 1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12288:Sample 1

tag: Namespace:UNIVERSITY OF MANCHESTER

characteristic: Submitter Id

unit: day

characteristic: age

text: ArrayExpress

characteristic: broker name

text: CCAP 211/8K

characteristic: cultivar

text: Protocols: C. sorokiniana cells (triplicates of irradiated and control treatments) were collected by centrifugation at 5000 g/5 min, washed 3× with ice-cold deionized water, snap-frozen in liquid N2, and stored at -80°C until further analysis. RNA extraction was performed by the addition of 1 mL TRIzol Reagent (Thermo Fisher Scientific, Waltham, MA USA), which was added to each sample and left to incubate at room temperature for 5 min. Chloroform (200 µL) was added to each sample for further extraction. Samples were briefly vortexed and left to stand for additional 5 min. To isolate RNA from DNA, proteins and other cell debris, the samples were centrifuged for 15 min at 12000 g and 4°C. The upper layer was then aliquoted, taking care not to disturb the lower layers. To precipitate the RNA, 500 µL of isopropanol was added to each sample and left for 15 min at room temperature. To collect the precipitated RNA, samples were centrifuged for 10 min at 12000 g and 4°C and the supernatant was removed. The RNA pellet was washed two times with 1 mL ethanol (75%) and centrifugation at 12000 g/5 min/4°C. Samples were left on ice for 30 min to allow any remaining ethanol to evaporate. The RNA pellet was then resuspended in 50 µL sterile deionized H2O. Chlorella sorokiniana strain CCAP 211/8K (alternative designation UTEX 1230) was obtained from the Culture Collection of Algae and Protozoa (CCAP), UK. C. sorokiniana was grown in 35 mL aliquots of 3N-BBM+V medium in 100 mL Erlenmeyer flasks that were placed on an orbital shaker (120 rpm) in a growth cabinet with temperature maintained at 22°C, and a continuous photon flux of 120 μmol m2/s. The medium was inoculated with 0.5 × 106 cells/mL. Microalgae were treated by a low dose of X-ray irradiation compared to non-irradiated control cells. Microalgae were irradiated at 20 days after inoculation, in the early stationary phase then RNA-Seq analysis was performed 24 hours after irradiation. Samples (35 mL) were placed in a Petri dish and exposed to a single continuous dose of X-rays using a CellRad system (FaxitronBioptics LLC, Tucson, AZ, USA), with the following settings: tube power, 750 W; filtration, 1.6 mm Be and 0.5 mm Al; energy, 120 kV, and using an irradiation dose of 5 Gy at a dose rate of 0.5 Gy/min, measured by using the built-in ion chamber dosimeter. Samples were rotated during irradiation. Irradiated cultures were placed back into 100 mL Erlenmeyer flasks and grown under the same growth conditions as before until cells were harvested for RNA extraction after 24 hours. Treated samples and controls (non-irradiated samples) were grown in the same batch. All experiments were performed in biological triplicates. Chlorella sorokiniana strain CCAP 211/8K (alternative designation UTEX 1230) was obtained from the Culture Collection of Algae and Protozoa (CCAP), UK. C. sorokiniana was grown in 35 mL aliquots of 3N-BBM+V medium in 100 mL Erlenmeyer flasks that were placed on an orbital shaker (120 rpm) in a growth cabinet with temperature maintained at 22°C, and a continuous photon flux of 120 μmol m2/s. The medium was inoculated with 0.5 × 106 cells/mL. Microalgae were treated by a low dose of X-ray irradiation compared to non-irradiated control cells. Microalgae were irradiated at 20 days after inoculation, in the early stationary phase then RNA-Seq analysis was performed 24 hours after irradiation. Samples (35 mL) were placed in a Petri dish and exposed to a single continuous dose of X-rays using a CellRad system (FaxitronBioptics LLC, Tucson, AZ, USA), with the following settings: tube power, 750 W; filtration, 1.6 mm Be and 0.5 mm Al; energy, 120 kV, and using an irradiation dose of 5 Gy at a dose rate of 0.5 Gy/min, measured by using the built-in ion chamber dosimeter. Samples were rotated during irradiation. Irradiated cultures were placed back into 100 mL Erlenmeyer flasks and grown under the same growth conditions as before until cells were harvested for RNA extraction after 24 hours. Treated samples and controls (non-irradiated samples) were grown in the same batch. All experiments were performed in biological triplicates. C. sorokiniana cells (triplicates of irradiated and control treatments) were collected by centrifugation at 5000 g/5 min, washed 3× with ice-cold deionized water, snap-frozen in liquid N2, and stored at -80°C until further analysis. RNA extraction was performed by the addition of 1 mL TRIzol Reagent (Thermo Fisher Scientific, Waltham, MA USA), which was added to each sample and left to incubate at room temperature for 5 min. Chloroform (200 µL) was added to each sample for further extraction. Samples were briefly vortexed and left to stand for additional 5 min. To isolate RNA from DNA, proteins and other cell debris, the samples were centrifuged for 15 min at 12000 g and 4°C. The upper layer was then aliquoted, taking care not to disturb the lower layers. To precipitate the RNA, 500 µL of isopropanol was added to each sample and left for 15 min at room temperature. To collect the precipitated RNA, samples were centrifuged for 10 min at 12000 g and 4°C and the supernatant was removed. The RNA pellet was washed two times with 1 mL ethanol (75%) and centrifugation at 12000 g/5 min/4°C. Samples were left on ice for 30 min to allow any remaining ethanol to evaporate. The RNA pellet was then resuspended in 50 µL sterile deionized H2O. TruSeq Stranded mRNA

text: wild type genotype

characteristic: genotype

text: not applicable

characteristic: isolate

text: Chlorella sorokiniana

text: Chlorella sorokiniana

text: whole organism

characteristic: organism part

text: Chlorella sorokiniana

text: Sample 1


E-MTAB-12288:Sample 2

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12288:Sample 2

text: ArrayExpress

text: CCAP 211/8K

characteristic: description

text: wild type genotype

text: not applicable

text: Chlorella sorokiniana

characteristic: organism

text: Chlorella sorokiniana

text: whole organism

text: Chlorella sorokiniana

text: Sample 2

characteristic: title


E-MTAB-12288:Sample 3

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12288:Sample 3

text: ArrayExpress

text: CCAP 211/8K

text: wild type genotype

text: not applicable

text: Chlorella sorokiniana

text: Chlorella sorokiniana

text: whole organism

text: Chlorella sorokiniana

text: Sample 3


E-MTAB-12288:Sample 4

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12288:Sample 4

text: ArrayExpress

text: CCAP 211/8K

text: wild type genotype

text: not applicable

text: Chlorella sorokiniana

text: Chlorella sorokiniana

text: whole organism

text: Chlorella sorokiniana

text: Sample 4


E-MTAB-12288:Sample 5

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12288:Sample 5

text: ArrayExpress

text: CCAP 211/8K

text: wild type genotype

text: not applicable

text: Chlorella sorokiniana

text: Chlorella sorokiniana

text: whole organism

text: Chlorella sorokiniana

text: Sample 5


E-MTAB-12288:Sample 6

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12288:Sample 6

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: CCAP 211/8K

text: wild type genotype

text: not applicable

text: Chlorella sorokiniana

text: Chlorella sorokiniana

text: whole organism

text: Chlorella sorokiniana

text: Sample 6


text: Natural History Museum

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

text: public

text: NATURAL HISTORY MUSEUM

characteristic: barcoding center

text: COPO

text: MAXWELL BARCLAY

text: NATURAL HISTORY MUSEUM

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Isle of Man|Scarlett Point

characteristic: geographic location (region and locality)

text: NOT COLLECTED

characteristic: habitat

text: MAXWELL BARCLAY

text: NATURAL HISTORY MUSEUM

text: adult

characteristic: lifestage

text: Petrobius brevistylis

text: WHOLE ORGANISM

text: DTOL

characteristic: project name

text: Hand picked

characteristic: sample collection device or method

text: Petrobius brevistylis

text: NOT PROVIDED

characteristic: sex

text: c049e5c9-f2d5-437e-a407-70132c2746ab-DTOL-specimen

characteristic: tolid

type: derived from


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Natural History Museum

text: NOT PROVIDED

text: EarlhamInstitute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: NATURAL HISTORY MUSEUM

text: COPO

text: MAXWELL BARCLAY

text: NATURAL HISTORY MUSEUM

text: United Kingdom

text: Isle of Man|Scarlett Point

text: NOT COLLECTED

text: DUNCAN SIVELL

text: NATURAL HISTORY MUSEUM

text: adult

text: Coelopa frigida

characteristic: organism

text: WHOLE ORGANISM

characteristic: organism part

text: DTOL

text: Hand picked

text: Coelopa frigida

text: NOT PROVIDED

text: 9cc0726f-04d1-49c4-93d3-9943fdd9d2c6-DTOL-specimen

characteristic: title


text: Natural History Museum

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

text: public

text: NATURAL HISTORY MUSEUM

characteristic: barcoding center

text: COPO

characteristic: broker name

text: BILL PARKER

text: NOT COLLECTED

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: England|Chertsey|Drill Hall Rd|Chertsey walk

characteristic: geographic location (region and locality)

text: NOT COLLECTED

characteristic: habitat

text: BILL PARKER

text: British Arachnological Society

text: adult

characteristic: lifestage

text: Larinioides sclopetarius

text: WHOLE ORGANISM

text: DTOL

characteristic: project name

text: Hand Picked

text: Larinioides sclopetarius

text: FEMALE

text: 58be3488-5eb7-4f34-b48c-8cf5ab44769e-DTOL-specimen


text: unknown

characteristic: ArrayExpress-SEX

text: Homo sapiens

characteristic: ArrayExpress-SPECIES

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Unknown

characteristic: GENDER

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: human

characteristic: common name

text: Homo sapiens

text: Homo sapiens


text: KYUNG HEE UNIVERSITY

text: public

tag: Namespace:KYUNG HEE UNIVERSITY

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: Japanese rice

text: Dongjin

characteristic: cultivar

text: Protocols: Anthers at the bicellular and tricellular stages of stages 11-13 were collected. Seeds of japonica rice cv. Dongjin was sterilized with 50% of sodium hypochlorite and germinated in MSO media in an incubator (14-h light/10-h dark, 28 °C (day)/22 °C (night), humidity 80%). After 1 week of germination, seedlings were transferred and grown in the greenhouse for a period of 4 weeks under growth chamber conditions and transferred to the field. Mature anthers were frozen in LN2 and finely ground using mortar. Grounded samples were homogenized using TRI reagents (RNAiso, Takara) and BCP (1-bromo-3-chloropropane). DNA, RNA, and protein layers were separated by centrifugation. Then, total RNA was extracted using the RNeasy Mini Kit (QIAGEN). The sequencing library is prepared by random fragmentation of the DNA or cDNA sample, followed by 5' and 3' adapter ligation. Alternatively, \"tagmentation\" combines the fragmentation and ligation reactions into a single step that greatly increases the efficiency of the library preparation process. Adapter-ligated fragments are then PCR amplified and gel purified.

characteristic: description

text: mixed J bicellular and tricellular pollen stage

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: field study

characteristic: growth condition

text: not applicable

characteristic: isolate

text: Oryza sativa Japonica Group

characteristic: organism

text: Oryza sativa Japonica Group

text: anther

characteristic: organism part

text: Oryza sativa Japonica Group

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: KYUNG HEE UNIVERSITY

characteristic: INSDC center name

text: public

text: ArrayExpress

text: Japanese rice

text: Dongjin

text: OsRALF17 -/-; OsRALF19 -/-

text: field study

text: not applicable

text: Oryza sativa Japonica Group

text: Oryza sativa Japonica Group

text: anther

text: Oryza sativa Japonica Group


name: SAMIN0000056-Whole Genome Sequencing of Nagina22

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Saurabh Raghuvanshi, University of Delhi South Campus

text: IBDC

text: Asian cultivated rice

characteristic: common name

text: Indian Drought tolerant variety - Nagina22

text: Oryza sativa

text: Oryza sativa

text: Whole Genome Sequencing of Nagina22


SAMIN0000057-Pusa Basmati-1

text: Saurabh Raghuvanshi, University of Delhi South Campus

text: public

text: SAMIN0000057-Pusa Basmati-1

characteristic: Submitter Id

text: IBDC

characteristic: broker name

text: Asian cultivated rice

text: high yielding drought sensitive indica basmati variety

characteristic: description

text: Oryza sativa

text: Oryza sativa

text: Pusa Basmati-1


text: public

text: IBDC

text: Asian cultivated rice

text: high yielding indica drought sensitive variety

text: Oryza sativa

text: Oryza sativa


name: SAMIN0000060-Oryza sativa subspe indica cv Vandana

text: public

text: IBDC

text: Asian cultivated rice

text: Oryza sativa

text: Oryza sativa

text: Oryza sativa subspe indica cv Vandana


SAMIN0000071-indica cultivar Anjali

text: public

text: SAMIN0000071-indica cultivar Anjali

text: IBDC

text: Asian cultivated rice

text: Oryza sativa subspe indica cvar Anjali, drought tolerant

text: Oryza sativa

text: Oryza sativa

text: indica cultivar Anjali


E-MTAB-12298:Sample 1

text: Co-author

text: public

text: E-MTAB-12298:Sample 1

text: ArrayExpress

text: adipocyte

text: house mouse

text: Protocols: 3T3-L1 fibroblasts were washed 3x in PBS and lysed in 350 µL RLT buffer. Lysed samples were collected and stored at -80°C until further analysis 3T3-L1 adipocytes were seeded and differentiated in 12-well plates. RNA extractions were performed using the RNeasy Mini Kit (Qiagen 1152 #74104) 1 μg of total RNA was quality checked (RIN >7) using an Agilent Bioanalyser 2100 system and then used to construct barcoded sequencing libraries with Illumina’s TruSeq Stranded mRNA Library Prep Kit following manufacturer’s instructions

text: embryo

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: high media volume

characteristic: growth condition

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: embryo

text: fibroblast

characteristic: progenitor cell type

text: Mus musculus

text: Sample 1


E-MTAB-12298:Sample 10

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Co-author

text: public

text: E-MTAB-12298:Sample 10

text: ArrayExpress

text: adipocyte

text: house mouse

text: embryo

text: wild type genotype

text: low media volume

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: embryo

characteristic: organism part

text: fibroblast

text: Mus musculus

text: Sample 10

characteristic: title


E-MTAB-12298:Sample 11

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Co-author

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-12298:Sample 11

text: ArrayExpress

text: adipocyte

text: house mouse

text: embryo

text: wild type genotype

text: high media volume

text: not applicable

text: Mus musculus

text: Mus musculus

text: embryo

text: fibroblast

text: Mus musculus

text: Sample 11


E-MTAB-12298:Sample 12

text: Co-author

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12298:Sample 12

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: adipocyte

text: house mouse

characteristic: common name

text: embryo

text: wild type genotype

text: low media volume

text: not applicable

text: Mus musculus

text: Mus musculus

text: embryo

text: fibroblast

text: Mus musculus

text: Sample 12


E-MTAB-12298:Sample 2

text: Co-author

text: public

text: E-MTAB-12298:Sample 2

text: ArrayExpress

text: adipocyte

text: house mouse

characteristic: description

text: embryo

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: low media volume

characteristic: growth condition

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: embryo

text: fibroblast

text: Mus musculus

text: Sample 2


E-MTAB-12298:Sample 3

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Co-author

text: public

text: E-MTAB-12298:Sample 3

text: ArrayExpress

text: adipocyte

text: house mouse

text: embryo

text: wild type genotype

text: high media volume

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: embryo

characteristic: organism part

text: fibroblast

characteristic: progenitor cell type

text: Mus musculus

text: Sample 3


E-MTAB-12298:Sample 4

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Co-author

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12298:Sample 4

text: ArrayExpress

text: adipocyte

text: house mouse

text: embryo

text: wild type genotype

text: low media volume

text: not applicable

text: Mus musculus

text: Mus musculus

text: embryo

text: fibroblast

text: Mus musculus

text: Sample 4

characteristic: title


E-MTAB-12298:Sample 5

text: Co-author

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12298:Sample 5

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: adipocyte

text: house mouse

text: embryo

text: wild type genotype

text: high media volume

text: not applicable

text: Mus musculus

text: Mus musculus

text: embryo

text: fibroblast

text: Mus musculus

text: Sample 5


E-MTAB-12298:Sample 6

text: Co-author

text: public

text: E-MTAB-12298:Sample 6

text: ArrayExpress

text: adipocyte

text: house mouse

characteristic: common name

characteristic: description

text: embryo

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: low media volume

characteristic: growth condition

text: not applicable

text: Mus musculus

text: Mus musculus

text: embryo

text: fibroblast

text: Mus musculus

text: Sample 6


E-MTAB-12298:Sample 7

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Co-author

text: public

text: E-MTAB-12298:Sample 7

text: ArrayExpress

text: adipocyte

text: house mouse

text: embryo

text: wild type genotype

text: high media volume

text: not applicable

characteristic: isolate

text: Mus musculus

characteristic: organism

text: Mus musculus

text: embryo

characteristic: organism part

text: fibroblast

text: Mus musculus

text: Sample 7