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text: metagenomic bin from biosample SAMEA4396468

characteristic: description

text: Ocean water

text: Ocean water

text: Ocean water

text: Atlantic Ocean

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: ocean water

text: marine metagenome

characteristic: metagenomic source

text: marine metagenome

text: marine metagenome

text: Illumina


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: spades.py --meta

text: metagenomic bin from biosample SAMEA2620812

text: Ocean water

text: Ocean water

text: Ocean water

text: Indian Ocean

text: metagenome

text: ocean water

text: marine metagenome

text: marine metagenome

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: marine metagenome

text: Illumina

characteristic: sequencing method

characteristic: title


text: ETH ZURICH

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: spades.py --meta

characteristic: assembly software

characteristic: binning parameters

text: metagenomic bin from biosample SAMEA2622837

text: Ocean water

characteristic: environment (biome)

text: Ocean water

characteristic: environment (feature)

text: Ocean water

characteristic: environment (material)

text: Pacific Ocean

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: metagenome

text: ocean water

text: marine metagenome

text: marine metagenome

text: marine metagenome

text: Illumina


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: spades.py --meta

characteristic: binning software

text: metagenomic bin from biosample SAMEA2621045

characteristic: description

text: Ocean water

text: Ocean water

text: Ocean water

text: Atlantic Ocean

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: ocean water

text: marine metagenome

characteristic: metagenomic source

text: marine metagenome

text: marine metagenome

text: Illumina


text: ETH ZURICH

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: spades.py --meta

characteristic: assembly software

characteristic: binning parameters

text: metagenomic bin from biosample SAMEA4398219

text: Ocean water

text: Ocean water

text: Ocean water

text: Arctic Ocean

text: metagenome

text: ocean water

text: marine metagenome

text: marine metagenome

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: marine metagenome

text: Illumina

characteristic: sequencing method

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: spades.py --meta

text: metagenomic bin from biosample SAMEA2620106

text: Ocean water

characteristic: environment (biome)

text: Ocean water

characteristic: environment (feature)

text: Ocean water

characteristic: environment (material)

text: Indian Ocean

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: metagenome

text: ocean water

text: marine metagenome

text: marine metagenome

text: marine metagenome

text: Illumina


text: ETH ZURICH

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: spades.py --meta

characteristic: assembly software

characteristic: binning parameters

characteristic: binning software

text: metagenomic bin from biosample SAMEA4397157

characteristic: description

text: Ocean water

text: Ocean water

text: Ocean water

text: Arctic Ocean

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: ocean water

text: marine metagenome

characteristic: metagenomic source

text: marine metagenome

text: marine metagenome

text: Illumina


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: spades.py --meta

text: tetra-nucleotide frequencies and co-abundance, --minContig 2000 --maxEdges 500

text: metagenomic bin from biosample SAMN07136652

text: Ocean water

text: Ocean water

text: Ocean water

text: Atlantic Ocean

text: metagenome

text: ocean water

text: marine metagenome

text: marine metagenome

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: marine metagenome

text: Illumina

characteristic: sequencing method

characteristic: title


text: ETH ZURICH

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: spades.py --meta

characteristic: assembly software

characteristic: binning parameters

text: metagenomic bin from biosample SAMN07136957

text: Ocean water

characteristic: environment (biome)

text: Ocean water

characteristic: environment (feature)

text: Ocean water

characteristic: environment (material)

text: Atlantic Ocean

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: metagenome

text: ocean water

text: marine metagenome

text: marine metagenome

text: marine metagenome

text: Illumina


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: spades.py --meta

characteristic: binning software

text: metagenomic bin from biosample SAMEA2623826

characteristic: description

text: Ocean water

text: Ocean water

text: Ocean water

text: Atlantic Ocean

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: ocean water

text: marine metagenome

characteristic: metagenomic source

text: marine metagenome

text: marine metagenome

text: Illumina


text: ETH ZURICH

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: spades.py --meta

characteristic: assembly software

characteristic: binning parameters

text: metagenomic bin from biosample SAMEA2621198

text: Ocean water

text: Ocean water

text: Ocean water

text: Atlantic Ocean

text: metagenome

text: ocean water

text: marine metagenome

text: marine metagenome

characteristic: organism

text: Biosynthetic potential of the global ocean microbiome

characteristic: project name

characteristic: sample derived from

text: marine metagenome

text: Illumina

characteristic: sequencing method

characteristic: title


text: unknown

characteristic: ArrayExpress-PHENOTYPE

text: unknown

characteristic: ArrayExpress-SEX

text: human

characteristic: ArrayExpress-SPECIES

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Unknown

characteristic: GENDER

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: unknown

characteristic: PHENOTYPE

text: human

characteristic: common name

text: Homo sapiens

text: Homo sapiens


HE 30A

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: HE 30A

tag: Namespace:GFZ German Research Centre for Geosciences

characteristic: Submitter Id

text: terrestrial biome

characteristic: broad-scale environmental context

characteristic: collection date

unit: m

characteristic: depth

text: 16S rRNA gene from anaerobic enrichment culture of eger rift drill core

characteristic: description

characteristic: elevation

text: sediment

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: subsurface

characteristic: local environmental context

text: soil metagenome

characteristic: organism

text: ICDP Eger

text: Zeyu 30a

text: soil metagenome

text: Illumina MiSeq v3 2x300bp

text: Hartousov enrichment 30 m replicate A


HE 30B

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: GFZ German Research Centre for Geosciences

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: HE 30B

text: terrestrial biome

text: sediment

text: Czech Republic

text: subsurface

text: soil metagenome

text: ICDP Eger

characteristic: project name

text: Zeyu 30b

text: soil metagenome

text: Illumina MiSeq v3 2x300bp

characteristic: sequencing method

text: Hartousov enrichment 30 m replicate B

characteristic: title


HE 30C

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: HE 30C

characteristic: Submitter Id

text: terrestrial biome

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

characteristic: description

text: sediment

text: Czech Republic

text: subsurface

text: soil metagenome

text: ICDP Eger

text: Zeyu 30c

text: soil metagenome

text: Illumina MiSeq v3 2x300bp

text: Hartousov enrichment 30 m replicate C


HE 30D

characteristic: ENA-CHECKLIST

text: public

text: HE 30D

text: terrestrial biome

characteristic: elevation

text: sediment

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: subsurface

characteristic: local environmental context

text: soil metagenome

characteristic: organism

text: ICDP Eger

text: Zeyu 30d

text: soil metagenome

text: Illumina MiSeq v3 2x300bp

text: Hartousov enrichment 30 m replicate D


HE 35A

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: HE 35A

text: terrestrial biome

text: sediment

text: Czech Republic

text: subsurface

text: soil metagenome

text: ICDP Eger

characteristic: project name

text: Zeyu 35a

text: soil metagenome

text: Illumina MiSeq v3 2x300bp

characteristic: sequencing method

text: Hartousov enrichment 35 m replicate A

characteristic: title


HE 35B

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: HE 35B

characteristic: Submitter Id

text: terrestrial biome

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

characteristic: description

text: sediment

text: Czech Republic

text: subsurface

text: soil metagenome

text: ICDP Eger

text: Zeyu 35b

text: soil metagenome

text: Illumina MiSeq v3 2x300bp

text: Hartousov enrichment 35 m replicate B


HE 35C

characteristic: ENA-CHECKLIST

text: public

text: HE 35C

text: terrestrial biome

characteristic: elevation

text: sediment

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: subsurface

characteristic: local environmental context

text: soil metagenome

characteristic: organism

text: ICDP Eger

text: Zeyu 35c

text: soil metagenome

text: Illumina MiSeq v3 2x300bp

text: Hartousov enrichment 35 m replicate C


HE 35D

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: HE 35D

text: terrestrial biome

text: sediment

text: Czech Republic

text: subsurface

text: soil metagenome

text: ICDP Eger

characteristic: project name

text: Zeyu 35d

text: soil metagenome

text: Illumina MiSeq v3 2x300bp

characteristic: sequencing method

text: Hartousov enrichment 35 m replicate D

characteristic: title


HE 54A

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: HE 54A

characteristic: Submitter Id

text: terrestrial biome

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

characteristic: description

text: sediment

text: Czech Republic

text: subsurface

text: soil metagenome

text: ICDP Eger

text: Zeyu 46a

text: soil metagenome

text: Illumina MiSeq v3 2x300bp

text: Hartousov enrichment 54 m replicate A


HE 54B

characteristic: ENA-CHECKLIST

text: public

text: HE 54B

text: terrestrial biome

characteristic: elevation

text: sediment

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: subsurface

characteristic: local environmental context

text: soil metagenome

characteristic: organism

text: ICDP Eger

text: Zeyu 46b

text: soil metagenome

text: Illumina MiSeq v3 2x300bp

text: Hartousov enrichment 54 m replicate B


HE 54C

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: HE 54C

text: terrestrial biome

text: sediment

text: Czech Republic

text: subsurface

text: soil metagenome

text: ICDP Eger

characteristic: project name

text: Zeyu 46c

text: soil metagenome

text: Illumina MiSeq v3 2x300bp

characteristic: sequencing method

text: Hartousov enrichment 54 m replicate C

characteristic: title


HE 54D

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: HE 54D

characteristic: Submitter Id

text: terrestrial biome

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

characteristic: description

text: sediment

text: Czech Republic

text: subsurface

text: soil metagenome

text: ICDP Eger

text: Zeyu 46d

text: soil metagenome

text: Illumina MiSeq v3 2x300bp

text: Hartousov enrichment 54 m replicate D


HE 60A

characteristic: ENA-CHECKLIST

text: public

text: HE 60A

text: terrestrial biome

characteristic: elevation

text: sediment

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: subsurface

characteristic: local environmental context

text: soil metagenome

characteristic: organism

text: ICDP Eger

text: Zeyu 48a

text: soil metagenome

text: Illumina MiSeq v3 2x300bp

text: Hartousov enrichment 60 m replicate A


HE 60B

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: HE 60B

text: terrestrial biome

text: sediment

text: Czech Republic

text: subsurface

text: soil metagenome

text: ICDP Eger

characteristic: project name

text: Zeyu 48b

text: soil metagenome

text: Illumina MiSeq v3 2x300bp

characteristic: sequencing method

text: Hartousov enrichment 60 m replicate B

characteristic: title


HE 60C

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: HE 60C

characteristic: Submitter Id

text: terrestrial biome

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

characteristic: description

text: sediment

text: Czech Republic

text: subsurface

text: soil metagenome

text: ICDP Eger

text: Zeyu 48c

text: soil metagenome

text: Illumina MiSeq v3 2x300bp

text: Hartousov enrichment 60 m replicate C


HE 60D

characteristic: ENA-CHECKLIST

text: public

text: HE 60D

text: terrestrial biome

characteristic: elevation

text: sediment

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: subsurface

characteristic: local environmental context

text: soil metagenome

characteristic: organism

text: ICDP Eger

text: Zeyu 48d

text: soil metagenome

text: Illumina MiSeq v3 2x300bp

text: Hartousov enrichment 60 m replicate D


HE 119A

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: HE 119A

text: terrestrial biome

text: sediment

text: Czech Republic

text: subsurface

text: soil metagenome

text: ICDP Eger

characteristic: project name

text: Zeyu 65a

text: soil metagenome

text: Illumina MiSeq v3 2x300bp

characteristic: sequencing method

text: Hartousov enrichment 119 m replicate A

characteristic: title


HE 119B

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: HE 119B

characteristic: Submitter Id

text: terrestrial biome

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

characteristic: description

text: sediment

text: Czech Republic

text: subsurface

text: soil metagenome

text: ICDP Eger

text: Zeyu 65b

text: soil metagenome

text: Illumina MiSeq v3 2x300bp

text: Hartousov enrichment 119 m replicate B


HE 119C

characteristic: ENA-CHECKLIST

text: public

text: HE 119C

text: terrestrial biome

characteristic: elevation

text: sediment

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: subsurface

characteristic: local environmental context

text: soil metagenome

characteristic: organism

text: ICDP Eger

text: Zeyu 65c

text: soil metagenome

text: Illumina MiSeq v3 2x300bp

text: Hartousov enrichment 119 m replicate C


HE 119D

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: HE 119D

text: terrestrial biome

text: sediment

text: Czech Republic

text: subsurface

text: soil metagenome

text: ICDP Eger

characteristic: project name

text: Zeyu 65d

text: soil metagenome

text: Illumina MiSeq v3 2x300bp

characteristic: sequencing method

text: Hartousov enrichment 119 m replicate D

characteristic: title


HE 136A

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: HE 136A

characteristic: Submitter Id

text: terrestrial biome

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

characteristic: description

text: sediment

text: Czech Republic

text: subsurface

text: soil metagenome

text: ICDP Eger

text: Zeyu 74a

text: soil metagenome

text: Illumina MiSeq v3 2x300bp

text: Hartousov enrichment 136 m replicate A


HE 136B

characteristic: ENA-CHECKLIST

text: public

text: HE 136B

text: terrestrial biome

characteristic: elevation

text: sediment

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: subsurface

characteristic: local environmental context

text: soil metagenome

characteristic: organism

text: ICDP Eger

text: Zeyu 74b

text: soil metagenome

text: Illumina MiSeq v3 2x300bp

text: Hartousov enrichment 136 m replicate B


HE 136C

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: HE 136C

text: terrestrial biome

text: sediment

text: Czech Republic

text: subsurface

text: soil metagenome

text: ICDP Eger

characteristic: project name

text: Zeyu 74c

text: soil metagenome

text: Illumina MiSeq v3 2x300bp

characteristic: sequencing method

text: Hartousov enrichment 136 m replicate C

characteristic: title


HE 136D

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: HE 136D

characteristic: Submitter Id

text: terrestrial biome

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

characteristic: description

text: sediment

text: Czech Republic

text: subsurface

text: soil metagenome

text: ICDP Eger

text: Zeyu 74d

text: soil metagenome

text: Illumina MiSeq v3 2x300bp

text: Hartousov enrichment 136 m replicate D


HE 222A

characteristic: ENA-CHECKLIST

text: public

text: HE 222A

text: terrestrial biome

characteristic: elevation

text: sediment

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: subsurface

characteristic: local environmental context

text: soil metagenome

characteristic: organism

text: ICDP Eger

text: Zeyu 112a

text: soil metagenome

text: Illumina MiSeq v3 2x300bp

text: Hartousov enrichment 222 m replicate A


HE 222B

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: HE 222B

text: terrestrial biome

text: sediment

text: Czech Republic

text: subsurface

text: soil metagenome

text: ICDP Eger

characteristic: project name

text: Zeyu 112b

text: soil metagenome

text: Illumina MiSeq v3 2x300bp

characteristic: sequencing method

text: Hartousov enrichment 222 m replicate B

characteristic: title


HE 222C

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: HE 222C

characteristic: Submitter Id

text: terrestrial biome

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

characteristic: description

text: sediment

text: Czech Republic

text: subsurface

text: soil metagenome

text: ICDP Eger

text: Zeyu 112c

text: soil metagenome

text: Illumina MiSeq v3 2x300bp

text: Hartousov enrichment 222 m replicate C


HE 222D

characteristic: ENA-CHECKLIST

text: public

text: HE 222D

text: terrestrial biome

characteristic: elevation

text: sediment

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: subsurface

characteristic: local environmental context

text: soil metagenome

characteristic: organism

text: ICDP Eger

text: Zeyu 112d

text: soil metagenome

text: Illumina MiSeq v3 2x300bp

text: Hartousov enrichment 222 m replicate D


BLKa

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: BLKa

text: negative control

text: Extraction Negtative Control 1

text: liquid

text: Germany

text: ground

text: soil metagenome

text: ICDP Eger

characteristic: project name

text: Zeyu BLKa

text: soil metagenome

text: Illumina MiSeq v3 2x300bp

characteristic: sequencing method

text: Extraction Blank A

characteristic: title


BLKb

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: BLKb

characteristic: Submitter Id

text: negative control

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

text: Extraction Negtative Control 2

characteristic: description

text: liquid

text: Germany

text: ground

text: soil metagenome

text: ICDP Eger

text: Zeyu BLKb

text: soil metagenome

text: Illumina MiSeq v3 2x300bp

text: Extraction Blank B


BLKc

characteristic: ENA-CHECKLIST

text: public

text: BLKc

text: negative control

text: Extraction Negtative Control 3

characteristic: elevation

text: liquid

characteristic: environmental medium

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: ground

characteristic: local environmental context

text: soil metagenome

characteristic: organism

text: ICDP Eger

text: Zeyu BLKc

text: soil metagenome

text: Illumina MiSeq v3 2x300bp

text: Extraction Blank C


BLKd

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: BLKd

text: negative control

text: Extraction Negtative Control 4

text: liquid

text: Germany

text: ground

text: soil metagenome

text: ICDP Eger

characteristic: project name

text: Zeyu BLKd

text: soil metagenome

text: Illumina MiSeq v3 2x300bp

characteristic: sequencing method

text: Extraction Blank D

characteristic: title


PCR NC

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: PCR NC

characteristic: Submitter Id

text: negative control

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

text: PCR Negative Control

characteristic: description

text: liquid

text: Germany

text: ground

text: soil metagenome

text: ICDP Eger

text: Zeyu PCR NC

text: soil metagenome

text: Illumina MiSeq v3 2x300bp

text: PCR NC


Mock log

characteristic: ENA-CHECKLIST

text: public

text: Mock log

text: negative control

text: Mock community log

characteristic: elevation

text: liquid

characteristic: environmental medium

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: ground

characteristic: local environmental context

text: soil metagenome

characteristic: organism

text: ICDP Eger

text: Mock log

text: soil metagenome

text: Illumina MiSeq v3 2x300bp

text: Mock community log


Mock even

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Mock even

text: negative control

text: Mock community even

text: liquid

text: Germany

text: ground

text: soil metagenome

text: ICDP Eger

characteristic: project name

text: Mock even

text: soil metagenome

text: Illumina MiSeq v3 2x300bp

characteristic: sequencing method

text: Mock community even

characteristic: title


text: Cell line

characteristic: ArrayExpress-PHENOTYPE

text: unknown

characteristic: ArrayExpress-SEX

text: Homo sapiens

characteristic: ArrayExpress-SPECIES

text: Unknown

characteristic: GENDER

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Cell line

characteristic: PHENOTYPE

characteristic: SRA accession

characteristic: Submitter Id

text: human

characteristic: common name

text: Homo sapiens

text: Homo sapiens


text: unidentified

text: Unknown

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: unidentified

text: unidentified

characteristic: organism

text: unidentified


text: unidentified

characteristic: ArrayExpress-Species

text: Unknown

characteristic: ArrayExpress-StrainOrLine

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: unidentified

text: unidentified

text: Mixed bacteria from nasopharyngeal swab

text: unidentified

text: Unknown

characteristic: strain


Caprella mutica

text: public

text: Caprella mutica

tag: Namespace:LOEWE-TBG (Translational Biodiversity Genomics)

text: Male

characteristic: description

text: Caprella mutica

text: Caprella mutica

text: Caprella mutica

characteristic: title


text: FONDAZIONE EDMUND MACH

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: DN_1_lib_11418AAD_ATACCGTGTCGGTCTCG_R1_001.fastq.gz

tag: Namespace:FONDAZIONE EDMUND MACH

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: FONDAZIONE EDMUND MACH

text: public

characteristic: SRA accession

text: DN_2_lib_11419AAD_TCTAGGTGTCGGTCTCG_R1_001.fastq.gz

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: FONDAZIONE EDMUND MACH

text: public

text: DN_3_lib_11420AAD_GCAGCGTGTCGGTCTCG_R1_001.fastq.gz

characteristic: Submitter Id

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: FONDAZIONE EDMUND MACH

text: public

text: DN_4_lib_11421AAD_CCGAGGTGTCGGTCTCG_R1_001.fastq.gz

text: baker's yeast

characteristic: common name

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


characteristic: ENA-CHECKLIST

text: FONDAZIONE EDMUND MACH

text: public

text: DN_5_lib_11422AAD_AAGATGTGTCGGTCTCG_R1_001.fastq.gz

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


characteristic: ENA-FIRST-PUBLIC

text: FONDAZIONE EDMUND MACH

text: public

text: DN_6_lib_11423AAD_TAGTAGTGTCGGTCTCG_R1_001.fastq.gz

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


characteristic: ENA-LAST-UPDATE

text: FONDAZIONE EDMUND MACH

text: public

text: DN_7_lib_11424AAD_GAACTGTGTCGGTCTCG_R1_001.fastq.gz

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: FONDAZIONE EDMUND MACH

text: public

text: DN_8_lib_11425AAD_CAATGGTGTCGGTCTCG_R1_001.fastq.gz

text: baker's yeast

text: Saccharomyces cerevisiae

characteristic: organism

text: Saccharomyces cerevisiae


text: FONDAZIONE EDMUND MACH

text: public

text: DN_9_lib_11426AAD_AGACAGTGTCGGTCTCG_R1_001.fastq.gz

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: FONDAZIONE EDMUND MACH

text: public

text: DN_10_lib_11427AAD_TGTTCGTGTCGGTCTCG_R1_001.fastq.gz

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: FONDAZIONE EDMUND MACH

text: public

text: DN_11_lib_11428AAD_GCCGGGTGTCGGTCTCG_R1_001.fastq.gz

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: FONDAZIONE EDMUND MACH

text: public

text: DN_12_lib_11429AAD_CGTAGGTGTCGGTCTCG_R1_001.fastq.gz

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: FONDAZIONE EDMUND MACH

text: public

text: DN_13_lib_11430AAD_ACACGGTGTCGGTCTCG_R1_001.fastq.gz

text: baker's yeast

text: Control

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: FONDAZIONE EDMUND MACH

text: public

text: DN_14_lib_11431AAD_TGTAAGTGTCGGTCTCG_R1_001.fastq.gz

text: baker's yeast

text: Control

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: FONDAZIONE EDMUND MACH

text: public

text: DN_15_lib_11432AAD_GTATTGTGTCGGTCTCG_R1_001.fastq.gz

text: baker's yeast

text: Control

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: FONDAZIONE EDMUND MACH

text: public

text: DN_16_lib_11433AAD_CTCTAGTGTCGGTCTCG_R1_001.fastq.gz

text: baker's yeast

text: Control

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: FONDAZIONE EDMUND MACH

text: public

text: UP_1_lib_11434AAD_ATACCGTCCACGAGGTC_R1_001.fastq.gz

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: FONDAZIONE EDMUND MACH

text: public

text: UP_2_lib_11435AAD_TCTAGGTCCACGAGGTC_R1_001.fastq.gz

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: FONDAZIONE EDMUND MACH

text: public

text: UP_3_lib_11436AAD_GCAGCGTCCACGAGGTC_R1_001.fastq.gz

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: FONDAZIONE EDMUND MACH

text: public

text: UP_4_lib_11437AAD_CCGAGGTCCACGAGGTC_R1_001.fastq.gz

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: FONDAZIONE EDMUND MACH

text: public

text: UP_5_lib_11438AAD_AAGATGTCCACGAGGTC_R1_001.fastq.gz

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: FONDAZIONE EDMUND MACH

text: public

text: UP_6_lib_11439AAD_TAGTAGTCCACGAGGTC_R1_001.fastq.gz

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: FONDAZIONE EDMUND MACH

text: public

text: UP_7_lib_11440AAD_GAACTGTCCACGAGGTC_R1_001.fastq.gz

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: FONDAZIONE EDMUND MACH

text: public

text: UP_8_lib_11441AAD_CAATGGTCCACGAGGTC_R1_001.fastq.gz

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: FONDAZIONE EDMUND MACH

text: public

text: UP_9_lib_11442AAD_AGACAGTCCACGAGGTC_R1_001.fastq.gz

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: FONDAZIONE EDMUND MACH

text: public

text: UP_10_lib_11443AAD_TGTTCGTCCACGAGGTC_R1_001.fastq.gz

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: FONDAZIONE EDMUND MACH

text: public

text: UP_11_lib_11444AAD_GCCGGGTCCACGAGGTC_R1_001.fastq.gz

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: FONDAZIONE EDMUND MACH

text: public

text: UP_12_lib_11445AAD_CGTAGGTCCACGAGGTC_R1_001.fastq.gz

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: FONDAZIONE EDMUND MACH

text: public

text: UP_13_lib_11446AAD_ACACGGTCCACGAGGTC_R1_001.fastq.gz

text: baker's yeast

text: Control

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: FONDAZIONE EDMUND MACH

text: public

text: UP_14_lib_11447AAD_TGTAAGTCCACGAGGTC_R1_001.fastq.gz

text: baker's yeast

text: Control

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: FONDAZIONE EDMUND MACH

text: public

text: UP_15_lib_11448AAD_GTATTGTCCACGAGGTC_R1_001.fastq.gz

text: baker's yeast

text: Control

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: FONDAZIONE EDMUND MACH

text: public

text: UP_16_lib_11449AAD_CTCTAGTCCACGAGGTC_R1_001.fastq.gz

text: baker's yeast

text: Control

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


BalbC

text: CANDIOLO CANCER INSTITUTE

text: public

text: BalbC

tag: Namespace:CANDIOLO CANCER INSTITUTE

text: house mouse

text: mouse cell line

text: Mus musculus

text: Mus musculus

text: Cell


text: CANDIOLO CANCER INSTITUTE

text: public

text: house mouse

text: mouse cell line

text: Mus musculus

text: Mus musculus

text: Cell


E-MTAB-12521:12h dissociated rep1

text: public

text: E-MTAB-12521:12h dissociated rep1

tag: Namespace:Walter and Eliza Hall Institute, Parkville, Australia

text: ArrayExpress

characteristic: broker name

text: thale cress

text: Protocols: Col-0 seeds were sown and collected at the 12 h, 24 h and 48 h time-points, in three independent replicates (batches) of the experiment. For bulk RNA-seq, embryos either collected without protoplasting (np) or with protoplasting (p). Col-0 seeds were sown on MS media plates (containing 3% sucrose). Seeds were sterilised and stratified for 48 h of cold (4°C) dark stratification before being transferred to continuous light (at 22°C), then collected after 12 h, 24 h and 48 h in the light. Dissociation of Arabidopsis thaliana embryos into single cells: Seeds were sandwiched between two glass slides and embryos were released mechanically from seed coats by pressing two slides. Ruptured seed coats and embryos were collected into microcentrifuge tubes and separated from each other using Percoll gradient. In brief, sample was resuspended in MC buffer (10mM potassium phospate pH7.0, 50mM NaCl, 0.1M sucrose) and loaded onto a 1ml 60% Percoll cushion. Sample was centrifuged at 800g for 5min and fraction of embryos (at the bottom of the tube) was collected and re-suspended in 0.6ml MC buffer. A second Percoll gradient was repeated to obtain pellet of embryos without any seed coats. Enriched embryos samples were cut with razor blades and digested in protoplasting buffer (2% w/v Cellulase, 3% w/v Macerozyme, 0.4M D-mannitol, 20mM MES, 20mM KCl in milli-Q water and adjust the pH to 5.7 with 1M Tris/HCL pH7.5, 0.1% w/v BSA, 10mM CaCl2, and 5mM β-mercaptoethanol). After 3 hour of digestion, protoplasts were filtered through a 70μm cell strainer, then 40μm cell strainer to remove debris and centrifuged at 500g for 5min. Supernatant was removed and pellet was washed with 2ml protoplasting buffer without enzymes and mercaptoethanol and centrifuged at 500g for 5min. Pellet was resuspended in 50μl protoplasting buffer without enzymes and mercaptoethanol. Protoplasts were counted using hemocytometer and adjusted to final concentration of 800-1200 protoplasts per μl. QIAgen Plant RNEasy kit Libraries were prepared using TruSeq Stranded Total RNA with Ribo-Zero Plant kit.

text: seed germination stage

characteristic: developmental stage

characteristic: ecotype

text: wild type genotype

characteristic: genotype

text: not applicable

characteristic: isolate

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: embryo

characteristic: organism part

characteristic: replicate

text: Arabidopsis thaliana

text: 12h dissociated rep1


E-MTAB-12521:12h dissociated rep2

characteristic: External Id

text: Walter and Eliza Hall Institute, Parkville, Australia

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12521:12h dissociated rep2

text: ArrayExpress

text: thale cress

characteristic: description

text: seed germination stage

text: wild type genotype

text: not applicable

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: embryo

text: Arabidopsis thaliana

text: 12h dissociated rep2

characteristic: title


E-MTAB-12521:12h dissociated rep3

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12521:12h dissociated rep3

characteristic: Submitter Id

text: ArrayExpress

text: thale cress

characteristic: common name

text: seed germination stage

text: wild type genotype

text: not applicable

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: embryo

text: Arabidopsis thaliana

text: 12h dissociated rep3


E-MTAB-12521:12h non-dissociated rep1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-12521:12h non-dissociated rep1

text: ArrayExpress

characteristic: broker name

text: thale cress

text: seed germination stage

text: wild type genotype

text: not applicable

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis thaliana

text: embryo

text: Arabidopsis thaliana

text: 12h non-dissociated rep1


E-MTAB-12521:12h non-dissociated rep2

text: public

text: E-MTAB-12521:12h non-dissociated rep2

text: ArrayExpress

text: thale cress

text: seed germination stage

characteristic: developmental stage

characteristic: ecotype

text: wild type genotype

characteristic: genotype

text: not applicable

characteristic: isolate

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: embryo

characteristic: organism part

text: Arabidopsis thaliana

text: 12h non-dissociated rep2


E-MTAB-12521:12h non-dissociated rep3

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12521:12h non-dissociated rep3

text: ArrayExpress

text: thale cress

characteristic: description

text: seed germination stage

text: wild type genotype

text: not applicable

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: embryo

characteristic: replicate

text: Arabidopsis thaliana

text: 12h non-dissociated rep3


E-MTAB-12521:24h dissociated rep1

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12521:24h dissociated rep1

characteristic: Submitter Id

text: ArrayExpress

text: thale cress

text: seed germination stage

text: wild type genotype

text: not applicable

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: embryo

text: Arabidopsis thaliana

text: 24h dissociated rep1

characteristic: title


E-MTAB-12521:24h dissociated rep2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-12521:24h dissociated rep2

text: ArrayExpress

characteristic: broker name

text: thale cress

characteristic: common name

text: seed germination stage

text: wild type genotype

text: not applicable

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: embryo

text: Arabidopsis thaliana

text: 24h dissociated rep2


E-MTAB-12521:24h dissociated rep3

text: public

text: E-MTAB-12521:24h dissociated rep3

text: ArrayExpress

text: thale cress

text: seed germination stage

characteristic: developmental stage

characteristic: ecotype

text: wild type genotype

characteristic: genotype

text: not applicable

characteristic: isolate

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis thaliana

text: embryo

text: Arabidopsis thaliana

text: 24h dissociated rep3


E-MTAB-12521:24h non-dissociated rep1

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12521:24h non-dissociated rep1

text: ArrayExpress

text: thale cress

characteristic: description

text: seed germination stage

text: wild type genotype

text: not applicable

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: embryo

characteristic: organism part

text: Arabidopsis thaliana

text: 24h non-dissociated rep1


E-MTAB-12521:24h non-dissociated rep2

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12521:24h non-dissociated rep2

characteristic: Submitter Id

text: ArrayExpress

text: thale cress

text: seed germination stage

text: wild type genotype

text: not applicable

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: embryo

characteristic: replicate

text: Arabidopsis thaliana

text: 24h non-dissociated rep2


E-MTAB-12521:24h non-dissociated rep3

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-12521:24h non-dissociated rep3

text: ArrayExpress

characteristic: broker name

text: thale cress

text: seed germination stage

text: wild type genotype

text: not applicable

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: embryo

text: Arabidopsis thaliana

text: 24h non-dissociated rep3

characteristic: title


E-MTAB-12521:48h dissociated rep1

text: public

text: E-MTAB-12521:48h dissociated rep1

text: ArrayExpress

text: thale cress

characteristic: common name

text: seed germination stage

characteristic: developmental stage

characteristic: ecotype

text: wild type genotype

characteristic: genotype

text: not applicable

characteristic: isolate

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: embryo

text: Arabidopsis thaliana

text: 48h dissociated rep1


E-MTAB-12521:48h dissociated rep2

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12521:48h dissociated rep2

text: ArrayExpress

text: thale cress

characteristic: description

text: seed germination stage

text: wild type genotype

text: not applicable

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis thaliana

text: embryo

text: Arabidopsis thaliana

text: 48h dissociated rep2


E-MTAB-12521:48h non-dissociated rep1

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12521:48h non-dissociated rep1

characteristic: Submitter Id

text: ArrayExpress

text: thale cress

text: seed germination stage

text: wild type genotype

text: not applicable

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: embryo

characteristic: organism part

text: Arabidopsis thaliana

text: 48h non-dissociated rep1


E-MTAB-12521:48h non-dissociated rep2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-12521:48h non-dissociated rep2

text: ArrayExpress

characteristic: broker name

text: thale cress

text: seed germination stage

text: wild type genotype

text: not applicable

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: embryo

characteristic: replicate

text: Arabidopsis thaliana

text: 48h non-dissociated rep2


E-MTAB-12521:48h non-dissociated rep3

text: public

text: E-MTAB-12521:48h non-dissociated rep3

text: ArrayExpress

text: thale cress

characteristic: common name

text: seed germination stage

characteristic: developmental stage

characteristic: ecotype

text: wild type genotype

characteristic: genotype

text: not applicable

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: embryo

text: Arabidopsis thaliana

text: 48h non-dissociated rep3

characteristic: title


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

tag: Namespace:DKTK Clinical Cooperation Unit (CCU) Neuroimmunology and Brain Tumor Immunology, German Cancer Research Center (DKFZ),

text: ArrayExpress

text: leukocyte

text: house mouse

text: Protocols: Mice were anesthetized and perfused with PBS. Brains of tumor-bearing mice were extracted and the tumor bearing hemisphere was cut into pieces and digested with 50 µg/ml liberase (Sigma) at 37°C for 30 minutes and subsequently mashed through a 100 µm and 70 µm strainer (Miltenyi). Myelin was removed using a 30% continuous Percoll (GE Healthcare) gradient and centrifugation at 2700 rpm. Immune cells were isolated as described above. Murine cells were blocked with rat anti-mouse CD16/32 (0.5 μg per well, eBioscience). Dextramer staining was performed according to manufacturer’s protocol (Immudex): after blocking, cells were incubated with 1:10 dextramer (PE and APC) dilution in 50 µl PBS for 10 minutes. Respective antibodies in PBS were added in 50 µl total volume and stained for 30 minutes. Following antibodies were used: CD45-BV510, CD3-FITC, MHCII-AF700, CD11b-PEDazzle, CD8-PercpCy5.5. eFluor 780 fixable viability dye (eBioscience) was used according to manufacturer's protocol to exclude dead cells. For scRNA-Seq, cells were pre-incubated for 10 minutes with titrated amounts of TotalSeq C hashing antibodies. Cells were sorted on a BDAria II and BD Aria Fusion through an 85 µM nozzle and 4-way purity. Cells were sorted in 20 µl 0.04% BSA in PBS and kept on ice until processing. Nuclei isolation was performed using the 10x Nuclei Isolation for Single Cell ATAC Sequencing protocol for low cell input. Cells were resuspended in chilled lysis buffer (10 mM Tris-HCl, 10 mM NaCl, 3 mM MgCl2, 0.1% Tween-20, 0.1% Nonidet P40 Substitute (Roche), 0.01% Digitonin (Sigma), 1% BSA (Miltenyi) in nuclease free water) and incubated on ice for 3 minutes. Cells were washed, resuspended in Nuclei Buffer (10x). 6.000-10.000 nuclei were used for subsequent single cell ATAC library preparation according to the 10x genomics ChromiumNext GEM Single Cell ATAC v1.1 user guide.

characteristic: description

text: glioblastoma

characteristic: disease

text: Cx3CR1CreERT2-MHCII flox

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: brain

text: Mus musculus

characteristic: strain


characteristic: ENA-CHECKLIST

text: DKTK Clinical Cooperation Unit (CCU) Neuroimmunology and Brain Tumor Immunology, German Cancer Research Center (DKFZ),

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: leukocyte

text: house mouse

text: glioblastoma

text: MHCII flox

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: brain

characteristic: organism part

text: Mus musculus


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

tag: Namespace:Institut d'Investigacio Sanitaria de Palma

text: laboratory mutant

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


text: Institut d'Investigacio Sanitaria de Palma

text: public

text: laboratory mutant

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa

characteristic: title


original sample FQSE10-0503

characteristic: External Id

text: public

text: original sample FQSE10-0503

text: clinical sample

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


original sample MAD05-008

characteristic: INSDC center name

text: public

text: original sample MAD05-008

text: clinical sample

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa


characteristic: INSDC first public

text: public

tag: Namespace:Cancer Stem Cells Unit, ISBReMIT, IRCSS Casa Sollievo della Sofferenza, Opera di San Pio da Pietrelcina

text: ArrayExpress

characteristic: broker name

text: Homo sapiens hospitalised patient

characteristic: clinical history

text: Protocols: Fecal samples were collected at baseline, after 12 weeks and 6 months of lifestyle intervention in tubes filled with DNA stabilization buffer (CANVAX Biotech). For 16S rRNA sequencing, the total microbial DNA was extracted using the QIAamp PowerFecal DNA Kit (Qiagen) following the manufacturer’s protocol. The Illumina 16S Metagenomic Sequencing Library Preparation for high-throughput sequencing was performed as follows: 12.5 ng of each DNA extract was employed for the amplification of the V3-V4 hypervariable regions of the bacterial 16S ribosomal RNA (rRNA) gene, using the universal primers previously published by Klindworth et al. with Illumina adapters.

text: breast cancer

text: gut metagenome

text: gut metagenome

text: feces

text: gut metagenome


text: Cancer Stem Cells Unit, ISBReMIT, IRCSS Casa Sollievo della Sofferenza, Opera di San Pio da Pietrelcina

text: public

text: ArrayExpress

text: Homo sapiens hospitalised patient

characteristic: description

text: breast cancer

characteristic: disease

text: gut metagenome

text: gut metagenome

text: feces

text: gut metagenome


E-MTAB-12502:Sample 1

characteristic: ENA-CHECKLIST

text: Martin-Luther-University Halle-Wittenberg

text: public

text: E-MTAB-12502:Sample 1

text: ArrayExpress

text: B cell

text: chronic viral infection

characteristic: clinical information

text: human

characteristic: common name

text: Protocols: Peripheral blood of HCV patients was collected in CPT tubes. PBMC were isolated by centrifugation and cryopreserved in liquid nitrogen. To enrich B cells for single-cell analyses, we FACS-sorted CD19+ cells. Cells were processed on a 10X Chromium Controller. Single-cell libraries were generated using the Next GEM Single Cell 5' Kit v2 and Chromium Single Cell Human BCR Amplification kits.

text: adult

characteristic: developmental stage

text: chronic hepatitis C virus infection

text: CD19 positive

characteristic: immunophenotype

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 1


E-MTAB-12502:Sample 2

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12502:Sample 2

characteristic: Submitter Id

text: ArrayExpress

text: B cell

text: chronic viral infection

text: human

text: adult

text: chronic hepatitis C virus infection

text: CD19 positive

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens

text: Sample 2


E-MTAB-12502:Sample 3

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12502:Sample 3

text: ArrayExpress

text: B cell

text: sustained viral response

text: human

text: adult

text: hepatitis C virus infection

text: CD19 positive

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 3

characteristic: title


E-MTAB-12502:Sample 4

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-12502:Sample 4

text: ArrayExpress

characteristic: broker name

text: B cell

text: sustained viral response

characteristic: clinical information

text: human

characteristic: description

text: adult

text: hepatitis C virus infection

characteristic: disease

text: CD19 positive

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 4


characteristic: INSDC last update

text: public

tag: Namespace:Lab.ricerca traslazionale AUSL-IRCCS di Reggio Emilia

text: ArrayExpress

text: human

characteristic: common name

text: Protocols: Cell were collected at 48h from transfection A549 cells were transfected with 30nM siRNA concentration. Total RNA from cell was extracted by Rneasy Mini kit (Qiagen) according to the manufacture’s instructions. Libraries were obtained starting from 100ng of total RNA following the Illumina Stranded Total RNA preparation Ligation with Ribo-Zero Plus protocol.

text: adult

characteristic: developmental stage

text: non-small cell lung adenocarcinoma

text: wild type genotype

characteristic: genotype

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: lung

text: Homo sapiens


text: Lab.ricerca traslazionale AUSL-IRCCS di Reggio Emilia

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: human

text: adult

text: non-small cell lung adenocarcinoma

text: wild type genotype

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: lung

characteristic: organism part

text: Homo sapiens


E-MTAB-12501:Sample 18

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INSTITUT CURIE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12501:Sample 18

tag: Namespace:INSTITUT CURIE

text: ArrayExpress

text: G1/S asynchronous

characteristic: cell cycle stage

text: HeLa

text: human

text: Protocols: For synchronized cells, a double thymidine block has been applied. For the 4sU-labeled RNA-seq, cells were cultured in 15 cm dish and 10 ml of new medium containing 1 mM 4sU was added for 8 minutes (Fuchs et al., 2015). HeLa cells were grown in DMEM 1X 4.5 g/L D-Glucose, L-Glutamine, Pyruvate (Gibco) with 10% Fetal Cow Serum FCS (Eurobio) and 1% Penicillin/Streptomycin (Gibco). Cells were transfected with siRNA at 100 nM concentration using Lipofectamine RNAiMAX (Invitrogen, 13778-150) as previously shown in (Clément et al., 2018) and for plasmids using Lipofectamine 2000 (Invitrogen, 11668-019). Cells were lysed, and total RNA was extracted directly from the cell culture dishes using the RNeasy Mini Kit according to the manufacturer’s instructions. The total RNA was DNase treatment using the TURBO DNA-free kit (ThermoFisher Scientific, AM1907) using manufacturer’s instructions. The quantity of isolated RNA was measured using the Nanodrop and quality was checked by Tapestation. For 4sU-labeled RNA purification, 100-200 μg RNA was used for RNA biotinylation and purification was performed using Streptavidin-coupled Dynabeads (Fuchs et al., 2015). 10 ng of total and 4sU-labeled RNA was used for library preparation using SMARTer Stranded Total RNA-seq kit.

text: adult

text: adenocarcinoma

text: nascent RNA

characteristic: fraction

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: mixed uterus and cervix

text: Homo sapiens

text: Sample 18


E-MTAB-12501:Sample 20

characteristic: ENA-CHECKLIST

text: INSTITUT CURIE

characteristic: INSDC last update

text: public

text: E-MTAB-12501:Sample 20

text: ArrayExpress

characteristic: broker name

text: G1/S asynchronous

text: HeLa

text: human

characteristic: description

text: adult

text: adenocarcinoma

characteristic: disease

text: nascent RNA

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: mixed uterus and cervix

text: Homo sapiens

text: Sample 20

characteristic: title


E-MTAB-12501:Sample 10

text: INSTITUT CURIE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12501:Sample 10

text: ArrayExpress

text: G1/S synchronized

text: HeLa

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: adenocarcinoma

text: total RNA

text: wild type genotype

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: mixed uterus and cervix

text: Homo sapiens

text: Sample 10


E-MTAB-12501:Sample 11

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: INSTITUT CURIE

text: public

text: E-MTAB-12501:Sample 11

characteristic: Submitter Id

text: ArrayExpress

text: G1/S synchronized

text: HeLa

text: human

text: adult

text: adenocarcinoma

text: total RNA

text: wild type genotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: mixed uterus and cervix

characteristic: organism part

text: Homo sapiens

text: Sample 11


E-MTAB-12501:Sample 12

characteristic: External Id

text: INSTITUT CURIE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12501:Sample 12

text: ArrayExpress

text: G1/S synchronized

characteristic: cell cycle stage

text: HeLa

text: human

text: adult

text: adenocarcinoma

text: total RNA

characteristic: fraction

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: mixed uterus and cervix

text: Homo sapiens

text: Sample 12


E-MTAB-12501:Sample 15

characteristic: ENA-CHECKLIST

text: INSTITUT CURIE

characteristic: INSDC last update

text: public

text: E-MTAB-12501:Sample 15

text: ArrayExpress

characteristic: broker name

text: G1/S asynchronous

text: HeLa

text: human

characteristic: description

text: adult

text: adenocarcinoma

characteristic: disease

text: total RNA

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: mixed uterus and cervix

text: Homo sapiens

text: Sample 15


E-MTAB-12501:Sample 16

text: INSTITUT CURIE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12501:Sample 16

text: ArrayExpress

text: G1/S asynchronous

text: HeLa

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: adenocarcinoma

text: total RNA

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: mixed uterus and cervix

text: Homo sapiens

text: Sample 16

characteristic: title


E-MTAB-12501:Sample 7

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: INSTITUT CURIE

text: public

text: E-MTAB-12501:Sample 7

characteristic: Submitter Id

text: ArrayExpress

text: G1/S synchronized

text: HeLa

text: human

text: adult

text: adenocarcinoma

text: total RNA

text: wild type genotype

characteristic: genotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: mixed uterus and cervix

text: Homo sapiens

text: Sample 7


E-MTAB-12501:Sample 8

characteristic: External Id

text: INSTITUT CURIE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12501:Sample 8

text: ArrayExpress

text: G1/S synchronized

characteristic: cell cycle stage

text: HeLa

text: human

text: adult

text: adenocarcinoma

text: total RNA

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: mixed uterus and cervix

characteristic: organism part

text: Homo sapiens

text: Sample 8


E-MTAB-12501:Sample 9

characteristic: ENA-CHECKLIST

text: INSTITUT CURIE

characteristic: INSDC last update

text: public

text: E-MTAB-12501:Sample 9

text: ArrayExpress

characteristic: broker name

text: G1/S synchronized

text: HeLa

text: human

characteristic: description

text: adult

text: adenocarcinoma

text: total RNA

characteristic: fraction

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: mixed uterus and cervix

text: Homo sapiens

text: Sample 9


E-MTAB-12501:Sample 17

text: INSTITUT CURIE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12501:Sample 17

text: ArrayExpress

text: G1/S asynchronous

text: HeLa

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: adenocarcinoma

characteristic: disease

text: nascent RNA

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: mixed uterus and cervix

text: Homo sapiens

text: Sample 17


E-MTAB-12501:Sample 19

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: INSTITUT CURIE

text: public

text: E-MTAB-12501:Sample 19

characteristic: Submitter Id

text: ArrayExpress

text: G1/S asynchronous

text: HeLa

text: human

text: adult

text: adenocarcinoma

text: nascent RNA

text: wild type genotype

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: mixed uterus and cervix

text: Homo sapiens

text: Sample 19

characteristic: title


E-MTAB-12501:Sample 1

characteristic: External Id

text: INSTITUT CURIE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12501:Sample 1

text: ArrayExpress

text: G1/S synchronized

characteristic: cell cycle stage

text: HeLa

text: human

text: adult

text: adenocarcinoma

text: total RNA

text: wild type genotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: mixed uterus and cervix

text: Homo sapiens

text: Sample 1


E-MTAB-12501:Sample 13

characteristic: ENA-CHECKLIST

text: INSTITUT CURIE

characteristic: INSDC last update

text: public

text: E-MTAB-12501:Sample 13

text: ArrayExpress

characteristic: broker name

text: G1/S asynchronous

text: HeLa

text: human

characteristic: description

text: adult

text: adenocarcinoma

text: total RNA

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: mixed uterus and cervix

characteristic: organism part

text: Homo sapiens

text: Sample 13


E-MTAB-12501:Sample 14

text: INSTITUT CURIE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12501:Sample 14

text: ArrayExpress

text: G1/S asynchronous

text: HeLa

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: adenocarcinoma

text: total RNA

characteristic: fraction

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: mixed uterus and cervix

text: Homo sapiens

text: Sample 14


E-MTAB-12501:Sample 2

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: INSTITUT CURIE

text: public

text: E-MTAB-12501:Sample 2

characteristic: Submitter Id

text: ArrayExpress

text: G1/S synchronized

text: HeLa

text: human

text: adult

text: adenocarcinoma

characteristic: disease

text: total RNA

text: wild type genotype

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: mixed uterus and cervix

text: Homo sapiens

text: Sample 2


E-MTAB-12501:Sample 3

characteristic: External Id

text: INSTITUT CURIE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12501:Sample 3

text: ArrayExpress

text: G1/S synchronized

characteristic: cell cycle stage

text: HeLa

text: human

text: adult

text: adenocarcinoma

text: total RNA

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: mixed uterus and cervix

text: Homo sapiens

text: Sample 3

characteristic: title


E-MTAB-12501:Sample 4

characteristic: ENA-CHECKLIST

text: INSTITUT CURIE

characteristic: INSDC last update

text: public

text: E-MTAB-12501:Sample 4

text: ArrayExpress

characteristic: broker name

text: G1/S synchronized

text: HeLa

text: human

characteristic: description

text: adult

text: adenocarcinoma

text: total RNA

text: wild type genotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: mixed uterus and cervix

text: Homo sapiens

text: Sample 4


E-MTAB-12501:Sample 5

text: INSTITUT CURIE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12501:Sample 5

text: ArrayExpress

text: G1/S synchronized

text: HeLa

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: adenocarcinoma

text: total RNA

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: mixed uterus and cervix

characteristic: organism part

text: Homo sapiens

text: Sample 5


E-MTAB-12501:Sample 6

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: INSTITUT CURIE

text: public

text: E-MTAB-12501:Sample 6

characteristic: Submitter Id

text: ArrayExpress

text: G1/S synchronized

text: HeLa

text: human

text: adult

text: adenocarcinoma

characteristic: disease

text: total RNA

characteristic: fraction

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: mixed uterus and cervix

text: Homo sapiens

text: Sample 6


E-MTAB-12520:Sample 10

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12520:Sample 10

tag: Namespace:Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen

unit: week

characteristic: age

text: ArrayExpress

text: house mouse

text: Protocols: The mice were fasted overnight (11 hours) prior to liver isolation, and livers were kept at -80°C until analysis. C57Bl/6JRj mice (8 weeks of age) received either a long acting glucagon analogue (NNC9204-0043; terminal half-life of 5-6 hours, 1.5 nmol/kg twice daily) or PBS+1% BSA (twice daily). Drugs were administered over a period of 8 weeks, by subcutaneous injection. The initial dose of the glucagon analogue of 3 nmol/kg was reduced to 1.5 nmol/kg after 16 days of treatment, due to a weight decrease of >20% of one of the mice (the mouse was terminated and excluded from the study). Raw sequencing data were processed using bcl2fastq software version 2.20.0 (Illumina). Total DNA/RNA was purified by an AllPrep DNA/RNA Minikit (catalog no. 80204; QIAgen, Hilden, Germany) according to manufacturer’s instructions and quality tested using a 2100 Bioanalyzer instrument (Agilent Genomics, Santa Clara, California, USA). The extracted RNA was DNase treated with RNeasy Minikit (catalog no. 74106; QIAgen, Hilden, Germany) and RNase-Free DNase set (catalog no. 79254; QIAgen, Hilden, Germany) according to manufacturer’s protocol. All the above-mentioned processes were performed on a QIACube machine (QIAgen, Hilden, Germany). Library preparation was done using TruSeq Stranded Total RNA Library Prep Gold (catalog no. 20020599; Illumina, San Diego, California, USA) and IDT for Illumina-TruSeq RNA UD Indexes (cat 20022371; Illumina, San Diego, California, USA) according to the manufacturer’s instructions

text: normal

text: wild type genotype

characteristic: genotype

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: kidney

text: Mus musculus

text: female

characteristic: sex

characteristic: strain

text: Sample 10


E-MTAB-12520:Sample 11

characteristic: ENA-CHECKLIST

text: Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12520:Sample 11

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: description

text: normal

text: wild type genotype

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: kidney

text: Mus musculus

text: female

text: Sample 11

characteristic: title


E-MTAB-12520:Sample 12

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-12520:Sample 12

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

characteristic: common name

text: normal

characteristic: disease

text: wild type genotype

text: not applicable

text: Mus musculus

text: Mus musculus

text: kidney

characteristic: organism part

text: Mus musculus

text: female

text: Sample 12


E-MTAB-12520:Sample 13

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12520:Sample 13

characteristic: age

text: ArrayExpress

text: house mouse

text: normal

text: wild type genotype

characteristic: genotype

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: kidney

text: Mus musculus

text: female

text: Sample 13


E-MTAB-12520:Sample 9

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12520:Sample 9

text: ArrayExpress

text: house mouse

text: normal

text: wild type genotype

text: not applicable

text: Mus musculus

text: Mus musculus

text: kidney

text: Mus musculus

text: female

characteristic: sex

characteristic: strain

text: Sample 9


E-MTAB-12520:Sample 17

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-12520:Sample 17

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: description

text: normal

text: wild type genotype

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: kidney

text: Mus musculus

text: female

text: Sample 17

characteristic: title