text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained A10

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained A10


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: GENGS-CBMSO

characteristic: INSDC center name

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characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: GENGS-CBMSO

characteristic: broker name

text: fruit fly

characteristic: common name

text: RUT Trained A11

characteristic: description

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: RUT Trained A11

characteristic: title


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained A12

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained A12


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained B01

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained B01


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained B02

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained B02


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained B03

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained B03


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained B04

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained B04


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained B05

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained B05


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained B06

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained B06


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained B07

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained B07


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained B08

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained B08


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained B09

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained B09


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained B10

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained B10


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained B11

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained B11


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained B12

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained B12


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained C01

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained C01


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained C02

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained C02


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained C03

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained C03


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained C04

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained C04


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained C05

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained C05


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained C06

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained C06


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained C07

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained C07


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained C08

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained C08


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained C09

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained C09


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained C10

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained C10


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained C11

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained C11


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained C12

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained C12


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained D01

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained D01


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained D02

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained D02


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained D03

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained D03


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained D04

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained D04


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained D05

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained D05


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained D06

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained D06


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained D07

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained D07


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained D08

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained D08


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained D09

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained D09


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained D10

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained D10


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained D11

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained D11


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained D12

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained D12


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained E01

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained E01


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained E02

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained E02


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained E03

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained E03


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained E04

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained E04


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained E05

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained E05


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained E06

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained E06


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained E07

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained E07


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained E08

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained E08


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: fruit fly

text: RUT Trained E09

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RUT Trained E09


text: STOCKHOLM UNIVERSITY

text: public

tag: Namespace:STOCKHOLM UNIVERSITY

characteristic: collection date

text: river trout

text: Salmo trutta pooled sample from one lake

text: Sweden

characteristic: geographic location (country and/or sea)

text: Salmo trutta

text: Salmo trutta


characteristic: ENA-CHECKLIST

text: AUSL-IRCCS di Reggio Emilia

text: public

text: E-MTAB-13043:12243_002_Segmented_Epithelioid

tag: Namespace:AUSL-IRCCS di Reggio Emilia

unit: year

characteristic: age

text: ArrayExpress

text: not provided

text: human

text: Protocols: Spatial Transcriptomic was performed by GeoMx DSP® (Nanostring technologies)starting from slides of 5μm FFPE tumor tissue. In order to perform spatial selection, slides were stained with GeoMx Solid Tumor TME Morphology Kit including antibodies against CytoKeratin (CK) and nuclear stain SYTO-13. For each slide, area of interest (AOI) were selected by two pathologists as representative of the tumor heterogeneity, based on morphology and expression of the Pan-CK marker. Three types of regions were selected: area including only epithelioid cells, area including only sarcomatoid cells and transitional regions containing both mixed epithelioid and sarcomatoids cells. These transitional regions were further segmented to separate the Epithelioid and sarcomatoid component. The slides were hybridized with the GeoMx® Cancer Transcriptome Atlas panel according to manufacturer protocol. After AOIs selection and photo-cleavage GeoMx RNA probes were sequenced. Hybridized RNA-probes were collected from each of the selected AOIs, and libraries for NGS sequencing were constructed by GeoMx® Seq Code Pack (Nanostring technologies). Libraries were pooled according to AOIs dimension, purified by AMPure XP beads (Beckman Coulter, Brea, Cali-fornia, USA) clean up and finally resuspended in a volume proportional to the number of pooled AOIs. The quality and quantity of library pools were evaluated by Agilent Bioanalyzer.

text: adult

characteristic: developmental stage

text: malignant pleural mesothelioma

characteristic: disease

text: not provided

characteristic: histology

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: pleura

characteristic: organism part

text: Homo sapiens

text: female

characteristic: sex


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: AUSL-IRCCS di Reggio Emilia

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13043:12243_002_Segmented_Sarcomatoid

characteristic: Submitter Id

text: ArrayExpress

text: not provided

text: human

text: adult

text: malignant pleural mesothelioma

text: not provided

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: pleura

text: Homo sapiens

text: female


characteristic: ENA-CHECKLIST

text: AUSL-IRCCS di Reggio Emilia

text: public

text: E-MTAB-13043:12243_003_Segmented_Epithelioid

characteristic: age

text: ArrayExpress

characteristic: broker name

text: not provided

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

text: malignant pleural mesothelioma

text: not provided

characteristic: geographic location (country and/or sea)

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: pleura

text: Homo sapiens

text: female

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: AUSL-IRCCS di Reggio Emilia

text: public

text: E-MTAB-13043:12243_003_Segmented_Sarcomatoid

text: ArrayExpress

text: not provided

text: human

text: adult

characteristic: developmental stage

text: malignant pleural mesothelioma

characteristic: disease

text: not provided

characteristic: histology

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: pleura

characteristic: organism part

text: Homo sapiens

text: female

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: External Id

text: AUSL-IRCCS di Reggio Emilia

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13043:12243_005_Segmented_Epithelioid

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: not provided

text: human

text: adult

text: malignant pleural mesothelioma

text: not provided

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: pleura

text: Homo sapiens

text: female


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: AUSL-IRCCS di Reggio Emilia

text: public

text: E-MTAB-13043:12243_006_Segmented_Sarcomatoid

text: ArrayExpress

characteristic: broker name

text: not provided

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

text: malignant pleural mesothelioma

text: not provided

characteristic: geographic location (country and/or sea)

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: pleura

text: Homo sapiens

text: female

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

text: AUSL-IRCCS di Reggio Emilia

text: public

text: E-MTAB-13043:12243_007_Segmented_Epithelioid

text: ArrayExpress

text: not provided

text: human

text: adult

characteristic: developmental stage

text: malignant pleural mesothelioma

characteristic: disease

text: not provided

characteristic: histology

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: pleura

characteristic: organism part

text: Homo sapiens

text: female

characteristic: sex


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: AUSL-IRCCS di Reggio Emilia

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13043:12243_007_Segmented_Sarcomatoid

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: not provided

text: human

text: adult

text: malignant pleural mesothelioma

text: not provided

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: pleura

text: Homo sapiens

text: female


characteristic: ENA-CHECKLIST

characteristic: External Id

text: AUSL-IRCCS di Reggio Emilia

text: public

text: E-MTAB-13043:12243_008_Segmented_Epithelioid

text: ArrayExpress

characteristic: broker name

text: not provided

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

text: malignant pleural mesothelioma

text: not provided

characteristic: geographic location (country and/or sea)

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: pleura

text: Homo sapiens

text: female

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: AUSL-IRCCS di Reggio Emilia

text: public

text: E-MTAB-13043:12243_008_Segmented_Sarcomatoid

text: ArrayExpress

text: not provided

text: human

text: adult

characteristic: developmental stage

text: malignant pleural mesothelioma

characteristic: disease

text: not provided

characteristic: histology

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: pleura

characteristic: organism part

text: Homo sapiens

text: female

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: External Id

text: AUSL-IRCCS di Reggio Emilia

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13043:12243_010_Segmented_Epithelioid

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: not provided

text: human

text: adult

text: malignant pleural mesothelioma

text: not provided

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: pleura

text: Homo sapiens

text: female


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: AUSL-IRCCS di Reggio Emilia

text: public

text: E-MTAB-13043:12243_010_Segmented_Sarcomatoid

text: ArrayExpress

characteristic: broker name

text: not provided

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

text: malignant pleural mesothelioma

text: not provided

characteristic: geographic location (country and/or sea)

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: pleura

text: Homo sapiens

text: female

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

text: AUSL-IRCCS di Reggio Emilia

text: public

text: E-MTAB-13043:12243_011_Segmented_Epithelioid

text: ArrayExpress

text: not provided

text: human

text: adult

characteristic: developmental stage

text: malignant pleural mesothelioma

characteristic: disease

text: not provided

characteristic: histology

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: pleura

characteristic: organism part

text: Homo sapiens

text: female

characteristic: sex


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: AUSL-IRCCS di Reggio Emilia

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13043:12243_012_Segmented_Epithelioid

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: not provided

text: human

text: adult

text: malignant pleural mesothelioma

text: not provided

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: pleura

text: Homo sapiens

text: female


characteristic: ENA-CHECKLIST

characteristic: External Id

text: AUSL-IRCCS di Reggio Emilia

text: public

text: E-MTAB-13043:12243_012_Segmented_Sarcomatoid

text: ArrayExpress

characteristic: broker name

text: not provided

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

text: malignant pleural mesothelioma

text: not provided

characteristic: geographic location (country and/or sea)

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: pleura

text: Homo sapiens

text: female

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: AUSL-IRCCS di Reggio Emilia

text: public

text: ArrayExpress

text: not provided

text: human

text: adult

characteristic: developmental stage

text: malignant pleural mesothelioma

characteristic: disease

text: not provided

characteristic: histology

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: pleura

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: External Id

text: AUSL-IRCCS di Reggio Emilia

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13043:14908_009_Segmented_Epithelioid

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: not provided

text: human

text: adult

text: malignant pleural mesothelioma

text: not provided

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: pleura

text: Homo sapiens

text: male


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: AUSL-IRCCS di Reggio Emilia

text: public

text: E-MTAB-13043:14908_009_Segmented_Sarcomatoid

text: ArrayExpress

characteristic: broker name

text: not provided

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

text: malignant pleural mesothelioma

text: not provided

characteristic: geographic location (country and/or sea)

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: pleura

text: Homo sapiens

text: male

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

text: AUSL-IRCCS di Reggio Emilia

text: public

text: E-MTAB-13043:14908_010_Segmented_Sarcomatoid

text: ArrayExpress

text: not provided

text: human

text: Protocols: Spatial Transcriptomic was performed by GeoMx DSP® (Nanostring technologies)starting from slides of 5μm FFPE tumor tissue. In order to perform spatial selection, slides were stained with GeoMx Solid Tumor TME Morphology Kit including antibodies against CytoKeratin (CK) and nuclear stain SYTO-13. For each slide, area of interest (AOI) were selected by two pathologists as representative of the tumor heterogeneity, based on morphology and expression of the Pan-CK marker. Three types of regions were selected: area including only epithelioid cells, area including only sarcomatoid cells and transitional regions containing both mixed epithelioid and sarcomatoids cells. These transitional regions were further segmented to separate the Epithelioid and sarcomatoid component. The slides were hybridized with the GeoMx® Cancer Transcriptome Atlas panel according to manufacturer protocol. After AOIs selection and photo-cleavage GeoMx RNA probes were sequenced. Hybridized RNA-probes were collected from each of the selected AOIs, and libraries for NGS sequencing were constructed by GeoMx® Seq Code Pack (Nanostring technologies). Libraries were pooled according to AOIs dimension, purified by AMPure XP beads (Beckman Coulter, Brea, Cali-fornia, USA) clean up and finally resuspended in a volume proportional to the number of pooled AOIs. The quality and quantity of library pools were evaluated by Agilent Bioanalyzer.

text: adult

characteristic: developmental stage

text: malignant pleural mesothelioma

characteristic: disease

text: not provided

characteristic: histology

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: pleura

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: AUSL-IRCCS di Reggio Emilia

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13043:14908_013_Segmented_Epithelioid

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: not provided

text: human

text: adult

text: malignant pleural mesothelioma

text: not provided

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: pleura

text: Homo sapiens

text: male


characteristic: ENA-CHECKLIST

characteristic: External Id

text: AUSL-IRCCS di Reggio Emilia

text: public

text: E-MTAB-13043:14908_013_Segmented_Sarcomatoid

text: ArrayExpress

characteristic: broker name

text: not provided

characteristic: collection date

text: human

characteristic: common name

characteristic: description

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text: Homo sapiens

characteristic: organism

text: Homo sapiens

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text: AUSL-IRCCS di Reggio Emilia

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text: E-MTAB-13043:14908_014_Segmented_Sarcomatoid

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text: Homo sapiens

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text: pleura

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text: Homo sapiens

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text: ArrayExpress

text: not provided

text: human

text: adult

text: malignant pleural mesothelioma

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text: pleura

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characteristic: ENA-FIRST-PUBLIC

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text: Homo sapiens

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text: Homo sapiens

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text: not applicable

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text: Homo sapiens

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characteristic: age

text: ArrayExpress

text: not provided

text: human

text: adult

text: malignant pleural mesothelioma

text: not provided

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text: Homo sapiens

text: Homo sapiens

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text: Homo sapiens

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text: Homo sapiens

text: pleura

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text: not applicable

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text: Homo sapiens

text: Homo sapiens

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text: Homo sapiens

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text: public

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text: ArrayExpress

text: not provided

text: human

text: adult

text: malignant pleural mesothelioma

text: not provided

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: pleura

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text: male


characteristic: ENA-FIRST-PUBLIC

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text: AUSL-IRCCS di Reggio Emilia

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text: not provided

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text: human

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text: adult

text: malignant pleural mesothelioma

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text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: pleura

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text: AUSL-IRCCS di Reggio Emilia

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text: not provided

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text: not provided

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text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: pleura

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text: Homo sapiens

text: male

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text: AUSL-IRCCS di Reggio Emilia

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text: E-MTAB-13043:15959_007_Segmented_Epithelioid

characteristic: Submitter Id

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text: ArrayExpress

text: not provided

text: human

text: adult

text: malignant pleural mesothelioma

text: not provided

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: pleura

text: Homo sapiens

text: male


characteristic: ENA-CHECKLIST

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text: AUSL-IRCCS di Reggio Emilia

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characteristic: broker name

text: not provided

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text: human

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text: adult

text: malignant pleural mesothelioma

text: not provided

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text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: pleura

text: Homo sapiens

text: male

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characteristic: ENA-FIRST-PUBLIC

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text: AUSL-IRCCS di Reggio Emilia

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text: E-MTAB-13043:15959_008_Segmented_Epithelioid

text: ArrayExpress

text: not provided

text: human

text: adult

characteristic: developmental stage

text: malignant pleural mesothelioma

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text: not provided

characteristic: histology

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: pleura

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex


characteristic: ENA-CHECKLIST

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text: AUSL-IRCCS di Reggio Emilia

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text: public

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text: E-MTAB-13043:15959_008_Segmented_Sarcomatoid

characteristic: Submitter Id

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text: ArrayExpress

text: not provided

text: human

text: adult

text: malignant pleural mesothelioma

text: not provided

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: pleura

text: Homo sapiens

text: male


characteristic: ENA-FIRST-PUBLIC

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text: AUSL-IRCCS di Reggio Emilia

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text: ArrayExpress

characteristic: broker name

text: not provided

characteristic: collection date

text: human

characteristic: common name

text: Protocols: Spatial Transcriptomic was performed by GeoMx DSP® (Nanostring technologies)starting from slides of 5μm FFPE tumor tissue. In order to perform spatial selection, slides were stained with GeoMx Solid Tumor TME Morphology Kit including antibodies against CytoKeratin (CK) and nuclear stain SYTO-13. For each slide, area of interest (AOI) were selected by two pathologists as representative of the tumor heterogeneity, based on morphology and expression of the Pan-CK marker. Three types of regions were selected: area including only epithelioid cells, area including only sarcomatoid cells and transitional regions containing both mixed epithelioid and sarcomatoids cells. These transitional regions were further segmented to separate the Epithelioid and sarcomatoid component. The slides were hybridized with the GeoMx® Cancer Transcriptome Atlas panel according to manufacturer protocol. After AOIs selection and photo-cleavage GeoMx RNA probes were sequenced. Hybridized RNA-probes were collected from each of the selected AOIs, and libraries for NGS sequencing were constructed by GeoMx® Seq Code Pack (Nanostring technologies). Libraries were pooled according to AOIs dimension, purified by AMPure XP beads (Beckman Coulter, Brea, Cali-fornia, USA) clean up and finally resuspended in a volume proportional to the number of pooled AOIs. The quality and quantity of library pools were evaluated by Agilent Bioanalyzer.

characteristic: description

text: adult

text: malignant pleural mesothelioma

text: not provided

characteristic: geographic location (country and/or sea)

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: pleura

text: Homo sapiens

text: male

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

text: AUSL-IRCCS di Reggio Emilia

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text: E-MTAB-13043:15959_010_Segmented_Epithelioid

text: ArrayExpress

text: not provided

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text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: pleura

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex


characteristic: ENA-FIRST-PUBLIC

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text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13043:15959_010_Segmented_Sarcomatoid

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: not provided

text: human

text: adult

text: malignant pleural mesothelioma

text: not provided

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: pleura

text: Homo sapiens

text: male


characteristic: ENA-CHECKLIST

characteristic: External Id

text: AUSL-IRCCS di Reggio Emilia

text: public

text: E-MTAB-13043:15959_011_Segmented_Epithelioid

text: ArrayExpress

characteristic: broker name

text: not provided

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

text: malignant pleural mesothelioma

text: not provided

characteristic: geographic location (country and/or sea)

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: pleura

text: Homo sapiens

text: male

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: AUSL-IRCCS di Reggio Emilia

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text: ArrayExpress

text: not provided

text: human

text: adult

characteristic: developmental stage

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text: not provided

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characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: pleura

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex


characteristic: ENA-CHECKLIST

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text: AUSL-IRCCS di Reggio Emilia

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text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13043:15959_012_Segmented_Sarcomatoid

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: not provided

text: human

text: adult

text: malignant pleural mesothelioma

text: not provided

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: pleura

text: Homo sapiens

text: male


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: AUSL-IRCCS di Reggio Emilia

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text: ArrayExpress

characteristic: broker name

text: not provided

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text: human

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text: adult

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text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: pleura

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text: not provided

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text: not provided

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characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: pleura

characteristic: organism part

text: Homo sapiens

text: male

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characteristic: ENA-FIRST-PUBLIC

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text: E-MTAB-13043:15959_014_Segmented_Epithelioid

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: not provided

text: human

text: adult

text: malignant pleural mesothelioma

text: not provided

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: pleura

text: Homo sapiens

text: male


characteristic: ENA-CHECKLIST

characteristic: External Id

text: AUSL-IRCCS di Reggio Emilia

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text: ArrayExpress

characteristic: broker name

text: not provided

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text: human

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text: malignant pleural mesothelioma

text: not provided

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text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: pleura

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text: male

characteristic: title


characteristic: ENA-FIRST-PUBLIC

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text: E-MTAB-13043:2158_001_Segmented_Epithelioid

text: ArrayExpress

text: not provided

text: human

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characteristic: developmental stage

text: malignant pleural mesothelioma

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text: not provided

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characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: pleura

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex


characteristic: ENA-CHECKLIST

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text: AUSL-IRCCS di Reggio Emilia

characteristic: INSDC center name

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text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13043:2158_003_Segmented_Epithelioid

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: not provided

text: human

text: adult

text: malignant pleural mesothelioma

text: not provided

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: pleura

text: Homo sapiens

text: male


characteristic: ENA-FIRST-PUBLIC

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text: ArrayExpress

characteristic: broker name

text: not provided

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text: human

characteristic: common name

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text: malignant pleural mesothelioma

text: not provided

characteristic: geographic location (country and/or sea)

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: pleura

text: Homo sapiens

text: male

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

text: AUSL-IRCCS di Reggio Emilia

text: public

text: E-MTAB-13043:2158_005_Segmented_Epithelioid

text: ArrayExpress

text: not provided

text: human

text: adult

characteristic: developmental stage

text: malignant pleural mesothelioma

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text: not provided

characteristic: histology

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text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: pleura

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex


characteristic: ENA-FIRST-PUBLIC

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text: AUSL-IRCCS di Reggio Emilia

characteristic: INSDC center name

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text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13043:2158_005_Segmented_Sarcomatoid

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: not provided

text: human

text: adult

text: malignant pleural mesothelioma

text: not provided

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: pleura

text: Homo sapiens

text: male


characteristic: ENA-CHECKLIST

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text: AUSL-IRCCS di Reggio Emilia

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text: ArrayExpress

characteristic: broker name

text: not provided

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text: human

characteristic: common name

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text: adult

text: malignant pleural mesothelioma

text: not provided

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text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: pleura

text: Homo sapiens

text: male

characteristic: title


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text: ArrayExpress

text: not provided

text: human

text: adult

characteristic: developmental stage

text: malignant pleural mesothelioma

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text: not provided

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characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: pleura

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex


characteristic: ENA-CHECKLIST

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text: AUSL-IRCCS di Reggio Emilia

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text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13043:2158_008_Segmented_Epithelioid

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: not provided

text: human

text: Protocols: Spatial Transcriptomic was performed by GeoMx DSP® (Nanostring technologies)starting from slides of 5μm FFPE tumor tissue. In order to perform spatial selection, slides were stained with GeoMx Solid Tumor TME Morphology Kit including antibodies against CytoKeratin (CK) and nuclear stain SYTO-13. For each slide, area of interest (AOI) were selected by two pathologists as representative of the tumor heterogeneity, based on morphology and expression of the Pan-CK marker. Three types of regions were selected: area including only epithelioid cells, area including only sarcomatoid cells and transitional regions containing both mixed epithelioid and sarcomatoids cells. These transitional regions were further segmented to separate the Epithelioid and sarcomatoid component. The slides were hybridized with the GeoMx® Cancer Transcriptome Atlas panel according to manufacturer protocol. After AOIs selection and photo-cleavage GeoMx RNA probes were sequenced. Hybridized RNA-probes were collected from each of the selected AOIs, and libraries for NGS sequencing were constructed by GeoMx® Seq Code Pack (Nanostring technologies). Libraries were pooled according to AOIs dimension, purified by AMPure XP beads (Beckman Coulter, Brea, Cali-fornia, USA) clean up and finally resuspended in a volume proportional to the number of pooled AOIs. The quality and quantity of library pools were evaluated by Agilent Bioanalyzer.

text: adult

text: malignant pleural mesothelioma

text: not provided

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: pleura

text: Homo sapiens

text: male


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: AUSL-IRCCS di Reggio Emilia

text: public

text: E-MTAB-13043:2158_008_Segmented_Sarcomatoid

text: ArrayExpress

characteristic: broker name

text: not provided

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

text: malignant pleural mesothelioma

text: not provided

characteristic: geographic location (country and/or sea)

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: pleura

text: Homo sapiens

text: male

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

text: AUSL-IRCCS di Reggio Emilia

text: public

text: E-MTAB-13043:2158_009_Segmented_Epithelioid

text: ArrayExpress

text: not provided

text: human

text: adult

characteristic: developmental stage

text: malignant pleural mesothelioma

characteristic: disease

text: not provided

characteristic: histology

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: pleura

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex


characteristic: ENA-FIRST-PUBLIC

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text: AUSL-IRCCS di Reggio Emilia

characteristic: INSDC center name

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text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13043:2158_009_Segmented_Sarcomatoid

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: not provided

text: human

text: adult

text: malignant pleural mesothelioma

text: not provided

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: pleura

text: Homo sapiens

text: male


characteristic: ENA-CHECKLIST

characteristic: External Id

text: AUSL-IRCCS di Reggio Emilia

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text: ArrayExpress

characteristic: broker name

text: not provided

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text: human

characteristic: common name

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text: adult

text: malignant pleural mesothelioma

text: not provided

characteristic: geographic location (country and/or sea)

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: pleura

text: Homo sapiens

text: male

characteristic: title


characteristic: ENA-FIRST-PUBLIC

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text: ArrayExpress

text: not provided

text: human

text: adult

characteristic: developmental stage

text: malignant pleural mesothelioma

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text: not provided

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characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: pleura

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex


characteristic: ENA-CHECKLIST

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text: AUSL-IRCCS di Reggio Emilia

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text: adult

text: malignant pleural mesothelioma

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text: Homo sapiens

text: Homo sapiens

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characteristic: ENA-FIRST-PUBLIC

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text: malignant pleural mesothelioma

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text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: pleura

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text: not provided

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text: not applicable

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text: Homo sapiens

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text: not provided

text: human

text: Protocols: Spatial Transcriptomic was performed by GeoMx DSP® (Nanostring technologies)starting from slides of 5μm FFPE tumor tissue. In order to perform spatial selection, slides were stained with GeoMx Solid Tumor TME Morphology Kit including antibodies against CytoKeratin (CK) and nuclear stain SYTO-13. For each slide, area of interest (AOI) were selected by two pathologists as representative of the tumor heterogeneity, based on morphology and expression of the Pan-CK marker. Three types of regions were selected: area including only epithelioid cells, area including only sarcomatoid cells and transitional regions containing both mixed epithelioid and sarcomatoids cells. These transitional regions were further segmented to separate the Epithelioid and sarcomatoid component. The slides were hybridized with the GeoMx® Cancer Transcriptome Atlas panel according to manufacturer protocol. After AOIs selection and photo-cleavage GeoMx RNA probes were sequenced. Hybridized RNA-probes were collected from each of the selected AOIs, and libraries for NGS sequencing were constructed by GeoMx® Seq Code Pack (Nanostring technologies). Libraries were pooled according to AOIs dimension, purified by AMPure XP beads (Beckman Coulter, Brea, Cali-fornia, USA) clean up and finally resuspended in a volume proportional to the number of pooled AOIs. The quality and quantity of library pools were evaluated by Agilent Bioanalyzer.

text: adult

text: malignant pleural mesothelioma

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text: Homo sapiens

text: Homo sapiens

text: pleura

text: Homo sapiens

text: male


characteristic: ENA-FIRST-PUBLIC

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text: not applicable

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text: Homo sapiens

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text: ArrayExpress

text: not provided

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text: adult

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text: not applicable

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text: ArrayExpress

text: not provided

text: human

text: adult

text: malignant pleural mesothelioma

text: not provided

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text: Homo sapiens

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characteristic: ENA-FIRST-PUBLIC

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text: Homo sapiens

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characteristic: ENA-CHECKLIST

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characteristic: broker name

text: not provided

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text: not provided

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text: not applicable

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text: not provided

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text: not provided

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text: adult

text: malignant pleural mesothelioma

text: not provided

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characteristic: ENA-FIRST-PUBLIC

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text: not applicable

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text: ArrayExpress

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text: not applicable

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text: pleura

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text: Homo sapiens

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text: ArrayExpress

text: not provided

text: human

text: adult

text: malignant pleural mesothelioma

text: not provided

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characteristic: broker name

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text: not provided

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text: not applicable

text: Homo sapiens

characteristic: organism

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text: pleura

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text: E-MTAB-13043:41231_002_Segmented_Sarcomatoid

characteristic: Submitter Id

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text: ArrayExpress

text: not provided

text: human

text: Protocols: Spatial Transcriptomic was performed by GeoMx DSP® (Nanostring technologies)starting from slides of 5μm FFPE tumor tissue. In order to perform spatial selection, slides were stained with GeoMx Solid Tumor TME Morphology Kit including antibodies against CytoKeratin (CK) and nuclear stain SYTO-13. For each slide, area of interest (AOI) were selected by two pathologists as representative of the tumor heterogeneity, based on morphology and expression of the Pan-CK marker. Three types of regions were selected: area including only epithelioid cells, area including only sarcomatoid cells and transitional regions containing both mixed epithelioid and sarcomatoids cells. These transitional regions were further segmented to separate the Epithelioid and sarcomatoid component. The slides were hybridized with the GeoMx® Cancer Transcriptome Atlas panel according to manufacturer protocol. After AOIs selection and photo-cleavage GeoMx RNA probes were sequenced. Hybridized RNA-probes were collected from each of the selected AOIs, and libraries for NGS sequencing were constructed by GeoMx® Seq Code Pack (Nanostring technologies). Libraries were pooled according to AOIs dimension, purified by AMPure XP beads (Beckman Coulter, Brea, Cali-fornia, USA) clean up and finally resuspended in a volume proportional to the number of pooled AOIs. The quality and quantity of library pools were evaluated by Agilent Bioanalyzer.

text: adult

text: malignant pleural mesothelioma

text: not provided

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: pleura

text: Homo sapiens

text: male


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: AUSL-IRCCS di Reggio Emilia

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text: ArrayExpress

characteristic: broker name

text: not provided

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text: malignant pleural mesothelioma

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text: not applicable

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characteristic: organism

text: Homo sapiens

text: pleura

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characteristic: ENA-CHECKLIST

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text: AUSL-IRCCS di Reggio Emilia

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text: E-MTAB-13043:41231_003_Segmented_Sarcomatoid

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text: not provided

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text: adult

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text: not applicable

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text: Homo sapiens

text: Homo sapiens

text: pleura

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characteristic: Submitter Id

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text: ArrayExpress

text: not provided

text: human

text: adult

text: malignant pleural mesothelioma

text: not provided

text: not applicable

text: Homo sapiens

text: Homo sapiens

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text: male


characteristic: ENA-CHECKLIST

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text: not applicable

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characteristic: organism

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text: not applicable

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text: ArrayExpress

text: not provided

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text: adult

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text: not provided

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text: Homo sapiens

text: Homo sapiens

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characteristic: ENA-FIRST-PUBLIC

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characteristic: organism

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text: Protocols: Spatial Transcriptomic was performed by GeoMx DSP® (Nanostring technologies)starting from slides of 5μm FFPE tumor tissue. In order to perform spatial selection, slides were stained with GeoMx Solid Tumor TME Morphology Kit including antibodies against CytoKeratin (CK) and nuclear stain SYTO-13. For each slide, area of interest (AOI) were selected by two pathologists as representative of the tumor heterogeneity, based on morphology and expression of the Pan-CK marker. Three types of regions were selected: area including only epithelioid cells, area including only sarcomatoid cells and transitional regions containing both mixed epithelioid and sarcomatoids cells. These transitional regions were further segmented to separate the Epithelioid and sarcomatoid component. The slides were hybridized with the GeoMx® Cancer Transcriptome Atlas panel according to manufacturer protocol. After AOIs selection and photo-cleavage GeoMx RNA probes were sequenced. Hybridized RNA-probes were collected from each of the selected AOIs, and libraries for NGS sequencing were constructed by GeoMx® Seq Code Pack (Nanostring technologies). Libraries were pooled according to AOIs dimension, purified by AMPure XP beads (Beckman Coulter, Brea, Cali-fornia, USA) clean up and finally resuspended in a volume proportional to the number of pooled AOIs. The quality and quantity of library pools were evaluated by Agilent Bioanalyzer.

characteristic: description

text: adult

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text: Protocols: Spatial Transcriptomic was performed by GeoMx DSP® (Nanostring technologies)starting from slides of 5μm FFPE tumor tissue. In order to perform spatial selection, slides were stained with GeoMx Solid Tumor TME Morphology Kit including antibodies against CytoKeratin (CK) and nuclear stain SYTO-13. For each slide, area of interest (AOI) were selected by two pathologists as representative of the tumor heterogeneity, based on morphology and expression of the Pan-CK marker. Three types of regions were selected: area including only epithelioid cells, area including only sarcomatoid cells and transitional regions containing both mixed epithelioid and sarcomatoids cells. These transitional regions were further segmented to separate the Epithelioid and sarcomatoid component. The slides were hybridized with the GeoMx® Cancer Transcriptome Atlas panel according to manufacturer protocol. After AOIs selection and photo-cleavage GeoMx RNA probes were sequenced. Hybridized RNA-probes were collected from each of the selected AOIs, and libraries for NGS sequencing were constructed by GeoMx® Seq Code Pack (Nanostring technologies). Libraries were pooled according to AOIs dimension, purified by AMPure XP beads (Beckman Coulter, Brea, Cali-fornia, USA) clean up and finally resuspended in a volume proportional to the number of pooled AOIs. The quality and quantity of library pools were evaluated by Agilent Bioanalyzer.

text: adult

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text: Homo sapiens

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characteristic: ENA-FIRST-PUBLIC

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characteristic: External Id

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