text: UBT

text: public

text: temperate biome [ENVO_01001831]

text: 16S rRNA V4 reads from the distal rhizosphere of Zea mays L. grown at an agricultural field in Ruhstorf, Germany. This sample was taken at the grain filling plant developmental stage and subjected to natural precipitation. Maize variety: Gelber Badischer Landmais.

text: 367 m

text: rhizosphere environment [ENVO_01000999]

text: YaraBela SULFAN (YARA GmbH & Co. KG, D�lmen, Germany)

text: Germany

text: Bavaria, Lower Bavaria, Pocking

text: 96 d

text: maize

text: grain filling

text: maize field [ENVO_01000348]

text: soil metagenome

text: not applicable

text: Rhizo4Bio: RhizoTraits

text: natural precipitation

text: -80 degC

text: soil metagenome

text: Illumina iSeq 100

text: variety:Gelber Badischer Landmais

text: 16S rRNA


status: PUBLIC

characteristic: ENA first public

tag: attribute

characteristic: ENA-CHECKLIST

text: UBT

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: temperate biome [ENVO_01001831]

characteristic: collection date

text: 16S rRNA V4 reads from bulk soil at an agricultural field in Ruhstorf, Germany. This sample was taken at the flowering plant developmental stage and subjected to natural precipitation. Maize variety: Braunes Schindelmeiser.

characteristic: description

text: 367 m

characteristic: elevation

text: bulk soil [ENVO_00005802]

text: Germany

characteristic: geographic location (country and/or sea)

text: Bavaria, Lower Bavaria, Pocking

text: Bromotril 225EC (ADAMA Agricultural Solutions, Ashdod, Israel), MaisTer power (Bayer CropScience Deutschland GmbH, Monheim, Germany)

text: 59 d

text: maize

text: flowering

text: maize field [ENVO_01000348]

text: Extraction with water instead of sample and PCR blank control.

text: soil metagenome

characteristic: organism

text: initial denaturation:98degC_120s; denaturation:98degC_10s; annealing:55degC_30s; extension:72degC_30s; final extension:72degC_120s

text: 515f: 5'-GTGYCAGCMGCCGCGGTAA-3', 806RB: 5'-GGACTACNVGGGTWTCTAAT-3'

text: not applicable

text: Bacterial mock community of the following taxa: Bacillus subtilis (NCBI:txid1423), Chryseobacterium luteum, Clostridium roseum (NCBI:txid421531), Enterobacter mori (NCBI:txid539813), Franconibacter helveticus (NCBI:txid357240), Glycomyces tenuis (NCBI:txid58116), Pseudomonas fluorescens (NCBI:txid294), Pseudomonas putida (NCBI:txid303), Rhizobium endophyticum (NCBI:txid578290), Streptomyces acidiscabies (NCBI:txid42234), Variovorax guangxiensis (NCBI:txid1775474). Amplicons were generated individually for each taxon with the same conditions as for the other samples and pooled in equimolar manner after amplification.

text: Rhizo4Bio: RhizoTraits

text: natural precipitation

text: -80 degC

text: soil metagenome

text: Illumina iSeq 100

text: variety:Braunes Schindelmeiser

text: 16S rRNA

text: prokaryotic microbiome in bulk soil

characteristic: title


text: UBT

text: public

text: temperate biome [ENVO_01001831]

text: 16S rRNA V4 reads from the proximal rhizosphere of Zea mays L. grown at an agricultural field in Ruhstorf, Germany. This sample was taken at the flowering plant developmental stage and subjected to natural precipitation. Maize variety: Braunes Schindelmeiser.

text: 367 m

text: rhizosphere environment [ENVO_01000999]

text: Germany

text: Bavaria, Lower Bavaria, Pocking

text: 59 d

text: maize

text: flowering

text: maize field [ENVO_01000348]

text: soil metagenome

text: not applicable

text: Rhizo4Bio: RhizoTraits

text: natural precipitation

text: -80 degC

text: soil metagenome

text: Illumina iSeq 100

text: variety:Braunes Schindelmeiser

text: 16S rRNA

text: prokaryotic microbiome in the proximal rhizosphere of Zea mays L.


text: UBT

text: public

text: temperate biome [ENVO_01001831]

text: 16S rRNA V4 reads from bulk soil at an agricultural field in Ruhstorf, Germany. This sample was taken at the grain filling plant developmental stage and subjected to natural precipitation. Maize variety: Braunes Schindelmeiser.

text: 367 m

text: bulk soil [ENVO_00005802]

text: Germany

text: Bavaria, Lower Bavaria, Pocking

text: 94 d

text: maize

text: grain filling

text: maize field [ENVO_01000348]

text: soil metagenome

text: not applicable

text: Rhizo4Bio: RhizoTraits

text: natural precipitation

text: -80 degC

text: soil metagenome

text: Illumina iSeq 100

text: variety:Braunes Schindelmeiser

text: 16S rRNA

text: prokaryotic microbiome in bulk soil


text: UBT

text: public

text: temperate biome [ENVO_01001831]

text: 16S rRNA V4 reads from roots (rhizoplane and endosphere) of Zea mays L. grown at an agricultural field in Ruhstorf, Germany. This sample was taken at the grain filling plant developmental stage and subjected to natural precipitation. Maize variety: Braunes Schindelmeiser.

text: 367 m

text: root [PO_0009005]

text: Germany

text: Bavaria, Lower Bavaria, Pocking

text: 94 d

text: maize

text: grain filling

text: maize field [ENVO_01000348]

text: root metagenome

text: Rhizo4Bio: RhizoTraits

text: natural precipitation

text: -80 degC

text: root metagenome

text: Illumina iSeq 100

text: variety:Braunes Schindelmeiser

text: 16S rRNA

text: prokaryotic microbiome in roots of Zea mays L.


text: UBT

text: public

text: temperate biome [ENVO_01001831]

text: 16S rRNA V4 reads from the proximal rhizosphere of Zea mays L. grown at an agricultural field in Ruhstorf, Germany. This sample was taken at the grain filling plant developmental stage and subjected to natural precipitation. Maize variety: Braunes Schindelmeiser.

text: 367 m

text: rhizosphere environment [ENVO_01000999]

text: Germany

text: Bavaria, Lower Bavaria, Pocking

text: 94 d

text: maize

text: grain filling

text: maize field [ENVO_01000348]

text: soil metagenome

text: not applicable

text: Rhizo4Bio: RhizoTraits

text: natural precipitation

text: -80 degC

text: soil metagenome

text: Illumina iSeq 100

text: variety:Braunes Schindelmeiser

text: 16S rRNA


text: UBT

text: public

text: temperate biome [ENVO_01001831]

text: 16S rRNA V4 reads from the distal rhizosphere of Zea mays L. grown at an agricultural field in Ruhstorf, Germany. This sample was taken at the grain filling plant developmental stage and subjected to natural precipitation. Maize variety: Braunes Schindelmeiser.

text: 367 m

text: rhizosphere environment [ENVO_01000999]

text: Germany

text: Bavaria, Lower Bavaria, Pocking

text: 94 d

text: maize

text: grain filling

text: maize field [ENVO_01000348]

text: soil metagenome

text: not applicable

text: Rhizo4Bio: RhizoTraits

text: natural precipitation

text: -80 degC

text: soil metagenome

text: Illumina iSeq 100

text: variety:Braunes Schindelmeiser

text: 16S rRNA

text: prokaryotic microbiome in the distal rhizosphere of Zea mays L.


text: UBT

text: public

text: temperate biome [ENVO_01001831]

text: 16S rRNA V4 reads from bulk soil at an agricultural field in Ruhstorf, Germany. This sample was taken at the flowering plant developmental stage and subjected to natural precipitation. Maize variety: Gelber Badischer Landmais.

text: 367 m

text: bulk soil [ENVO_00005802]

text: Germany

text: Bavaria, Lower Bavaria, Pocking

text: 67 d

text: maize

text: flowering

text: maize field [ENVO_01000348]

text: soil metagenome

text: not applicable

text: Rhizo4Bio: RhizoTraits

text: natural precipitation

text: -80 degC

text: soil metagenome

text: Illumina iSeq 100

text: variety:Gelber Badischer Landmais

text: 16S rRNA

text: prokaryotic microbiome in bulk soil


text: UBT

text: public

text: temperate biome [ENVO_01001831]

text: 16S rRNA V4 reads from roots (rhizoplane and endosphere) of Zea mays L. grown at an agricultural field in Ruhstorf, Germany. This sample was taken at the flowering plant developmental stage and subjected to natural precipitation. Maize variety: Gelber Badischer Landmais.

text: 367 m

text: root [PO_0009005]

text: Germany

text: Bavaria, Lower Bavaria, Pocking

text: 67 d

text: maize

text: flowering

text: maize field [ENVO_01000348]

text: root metagenome

text: Rhizo4Bio: RhizoTraits

text: natural precipitation

text: -80 degC

text: root metagenome

text: Illumina iSeq 100

text: variety:Gelber Badischer Landmais

text: 16S rRNA


text: UBT

text: public

text: temperate biome [ENVO_01001831]

text: 16S rRNA V4 reads from the proximal rhizosphere of Zea mays L. grown at an agricultural field in Ruhstorf, Germany. This sample was taken at the flowering plant developmental stage and subjected to natural precipitation. Maize variety: Gelber Badischer Landmais.

text: 367 m

text: rhizosphere environment [ENVO_01000999]

text: YaraBela SULFAN (YARA GmbH & Co. KG, D�lmen, Germany)

text: Germany

text: Bavaria, Lower Bavaria, Pocking

text: 67 d

text: maize

text: flowering

text: maize field [ENVO_01000348]

text: soil metagenome

text: not applicable

text: Rhizo4Bio: RhizoTraits

text: natural precipitation

text: -80 degC

text: soil metagenome

text: Illumina iSeq 100

text: variety:Gelber Badischer Landmais

text: 16S rRNA


text: UBT

text: public

text: temperate biome [ENVO_01001831]

text: 16S rRNA V4 reads from bulk soil at an agricultural field in Ruhstorf, Germany. This sample was taken at the grain filling plant developmental stage and subjected to natural precipitation. Maize variety: Gelber Badischer Landmais.

text: 367 m

text: bulk soil [ENVO_00005802]

text: Germany

text: Bavaria, Lower Bavaria, Pocking

text: Bromotril 225EC (ADAMA Agricultural Solutions, Ashdod, Israel), MaisTer power (Bayer CropScience Deutschland GmbH, Monheim, Germany)

text: 92 d

text: maize

text: grain filling

text: maize field [ENVO_01000348]

text: soil metagenome

text: not applicable

text: Rhizo4Bio: RhizoTraits

text: natural precipitation

text: -80 degC

text: soil metagenome

text: Illumina iSeq 100

text: variety:Gelber Badischer Landmais

text: 16S rRNA

text: prokaryotic microbiome in bulk soil


text: UBT

text: public

text: temperate biome [ENVO_01001831]

text: 16S rRNA V4 reads from roots (rhizoplane and endosphere) of Zea mays L. grown at an agricultural field in Ruhstorf, Germany. This sample was taken at the grain filling plant developmental stage and subjected to natural precipitation. Maize variety: Gelber Badischer Landmais.

text: 367 m

text: root [PO_0009005]

text: Germany

text: Bavaria, Lower Bavaria, Pocking

text: 92 d

text: maize

text: grain filling

text: maize field [ENVO_01000348]

text: Extraction with water instead of sample and PCR blank control.

text: root metagenome

text: initial denaturation:98degC_120s; denaturation:98degC_10s; annealing:55degC_30s; extension:72degC_30s; final extension:72degC_120s

text: Rhizo4Bio: RhizoTraits

text: natural precipitation

text: -80 degC

text: root metagenome

text: Illumina iSeq 100

text: variety:Gelber Badischer Landmais

text: 16S rRNA


text: UBT

text: public

text: temperate biome [ENVO_01001831]

text: 16S rRNA V4 reads from the proximal rhizosphere of Zea mays L. grown at an agricultural field in Ruhstorf, Germany. This sample was taken at the grain filling plant developmental stage and subjected to natural precipitation. Maize variety: Gelber Badischer Landmais.

text: 367 m

text: rhizosphere environment [ENVO_01000999]

text: Germany

text: Bavaria, Lower Bavaria, Pocking

text: 92 d

text: maize

text: grain filling

text: maize field [ENVO_01000348]

text: soil metagenome

text: 515f: 5'-GTGYCAGCMGCCGCGGTAA-3', 806RB: 5'-GGACTACNVGGGTWTCTAAT-3'

text: not applicable

text: Bacterial mock community of the following taxa: Bacillus subtilis (NCBI:txid1423), Chryseobacterium luteum, Clostridium roseum (NCBI:txid421531), Enterobacter mori (NCBI:txid539813), Franconibacter helveticus (NCBI:txid357240), Glycomyces tenuis (NCBI:txid58116), Pseudomonas fluorescens (NCBI:txid294), Pseudomonas putida (NCBI:txid303), Rhizobium endophyticum (NCBI:txid578290), Streptomyces acidiscabies (NCBI:txid42234), Variovorax guangxiensis (NCBI:txid1775474). Amplicons were generated individually for each taxon with the same conditions as for the other samples and pooled in equimolar manner after amplification.

text: Rhizo4Bio: RhizoTraits

text: natural precipitation

text: -80 degC

text: soil metagenome

text: Illumina iSeq 100

text: variety:Gelber Badischer Landmais

text: 16S rRNA


text: UBT

text: public

text: temperate biome [ENVO_01001831]

text: 16S rRNA V4 reads from the distal rhizosphere of Zea mays L. grown at an agricultural field in Ruhstorf, Germany. This sample was taken at the grain filling plant developmental stage and subjected to natural precipitation. Maize variety: Gelber Badischer Landmais.

text: 367 m

text: rhizosphere environment [ENVO_01000999]

text: Germany

text: Bavaria, Lower Bavaria, Pocking

text: 92 d

text: maize

text: grain filling

text: maize field [ENVO_01000348]

text: soil metagenome

text: not applicable

text: Rhizo4Bio: RhizoTraits

text: natural precipitation

text: -80 degC

text: soil metagenome

text: Illumina iSeq 100

text: variety:Gelber Badischer Landmais

text: 16S rRNA


text: UBT

text: public

text: temperate biome [ENVO_01001831]

text: 16S rRNA V4 reads from roots (rhizoplane and endosphere) of Zea mays L. grown at an agricultural field in Ruhstorf, Germany. This sample was taken at the flowering plant developmental stage and subjected to natural precipitation. Maize variety: Braunes Schindelmeiser.

text: 367 m

text: root [PO_0009005]

text: Germany

text: Bavaria, Lower Bavaria, Pocking

text: 59 d

text: maize

text: flowering

text: maize field [ENVO_01000348]

text: root metagenome

text: Rhizo4Bio: RhizoTraits

text: natural precipitation

text: -80 degC

text: root metagenome

text: Illumina iSeq 100

text: variety:Braunes Schindelmeiser

text: 16S rRNA

text: prokaryotic microbiome in roots of Zea mays L.


text: UBT

text: public

text: temperate biome [ENVO_01001831]

text: 16S rRNA V4 reads from bulk soil at an agricultural field in Ruhstorf, Germany. This sample was taken at the grain filling plant developmental stage and subjected to natural precipitation. Maize variety: Braunes Schindelmeiser.

text: 367 m

text: bulk soil [ENVO_00005802]

text: Germany

text: Bavaria, Lower Bavaria, Pocking

text: 93 d

text: maize

text: grain filling

text: maize field [ENVO_01000348]

text: soil metagenome

text: not applicable

text: Rhizo4Bio: RhizoTraits

text: natural precipitation

text: -80 degC

text: soil metagenome

text: Illumina iSeq 100

text: variety:Braunes Schindelmeiser

text: 16S rRNA

text: prokaryotic microbiome in bulk soil


text: UBT

text: public

text: temperate biome [ENVO_01001831]

text: 16S rRNA V4 reads from roots (rhizoplane and endosphere) of Zea mays L. grown at an agricultural field in Ruhstorf, Germany. This sample was taken at the grain filling plant developmental stage and subjected to natural precipitation. Maize variety: Braunes Schindelmeiser.

text: 367 m

text: root [PO_0009005]

text: Germany

text: Bavaria, Lower Bavaria, Pocking

text: 93 d

text: maize

text: grain filling

text: maize field [ENVO_01000348]

text: root metagenome

text: Rhizo4Bio: RhizoTraits

text: natural precipitation

text: -80 degC

text: root metagenome

text: Illumina iSeq 100

text: variety:Braunes Schindelmeiser

text: 16S rRNA


text: UBT

text: public

text: temperate biome [ENVO_01001831]

text: 16S rRNA V4 reads from the proximal rhizosphere of Zea mays L. grown at an agricultural field in Ruhstorf, Germany. This sample was taken at the grain filling plant developmental stage and subjected to natural precipitation. Maize variety: Braunes Schindelmeiser.

text: 367 m

text: rhizosphere environment [ENVO_01000999]

text: Germany

text: Bavaria, Lower Bavaria, Pocking

text: 93 d

text: maize

text: grain filling

text: maize field [ENVO_01000348]

text: soil metagenome

text: not applicable

text: Rhizo4Bio: RhizoTraits

text: natural precipitation

text: -80 degC

text: soil metagenome

text: Illumina iSeq 100

text: variety:Braunes Schindelmeiser

text: 16S rRNA

text: prokaryotic microbiome in the proximal rhizosphere of Zea mays L.


text: UBT

text: public

text: temperate biome [ENVO_01001831]

text: 16S rRNA V4 reads from the distal rhizosphere of Zea mays L. grown at an agricultural field in Ruhstorf, Germany. This sample was taken at the grain filling plant developmental stage and subjected to natural precipitation. Maize variety: Braunes Schindelmeiser.

text: 367 m

text: rhizosphere environment [ENVO_01000999]

text: Germany

text: Bavaria, Lower Bavaria, Pocking

text: 93 d

text: maize

text: grain filling

text: maize field [ENVO_01000348]

text: soil metagenome

text: not applicable

text: Rhizo4Bio: RhizoTraits

text: natural precipitation

text: -80 degC

text: soil metagenome

text: Illumina iSeq 100

text: variety:Braunes Schindelmeiser

text: 16S rRNA

text: prokaryotic microbiome in the distal rhizosphere of Zea mays L.


text: UBT

text: public

text: temperate biome [ENVO_01001831]

text: 16S rRNA V4 reads from bulk soil at an agricultural field in Ruhstorf, Germany. This sample was taken at the flowering plant developmental stage and subjected to natural precipitation reduced by 60%. Maize variety: Gelber Badischer Landmais.

text: 367 m

text: bulk soil [ENVO_00005802]

text: YaraBela SULFAN (YARA GmbH & Co. KG, D�lmen, Germany)

text: Germany

text: Bavaria, Lower Bavaria, Pocking

text: 67 d

text: maize

text: flowering

text: maize field [ENVO_01000348]

text: soil metagenome

text: not applicable

text: Rhizo4Bio: RhizoTraits

text: natural precipitation reduced by 60%

text: -80 degC

text: soil metagenome

text: Illumina iSeq 100

text: variety:Gelber Badischer Landmais

text: 16S rRNA

text: prokaryotic microbiome in bulk soil


text: UBT

text: public

text: temperate biome [ENVO_01001831]

text: 16S rRNA V4 reads from roots (rhizoplane and endosphere) of Zea mays L. grown at an agricultural field in Ruhstorf, Germany. This sample was taken at the flowering plant developmental stage and subjected to natural precipitation reduced by 60%. Maize variety: Gelber Badischer Landmais.

text: 367 m

text: root [PO_0009005]

text: Germany

text: Bavaria, Lower Bavaria, Pocking

text: Bromotril 225EC (ADAMA Agricultural Solutions, Ashdod, Israel), MaisTer power (Bayer CropScience Deutschland GmbH, Monheim, Germany)

text: 67 d

text: maize

text: flowering

text: maize field [ENVO_01000348]

text: root metagenome

text: Rhizo4Bio: RhizoTraits

text: natural precipitation reduced by 60%

text: -80 degC

text: root metagenome

text: Illumina iSeq 100

text: variety:Gelber Badischer Landmais

text: 16S rRNA


text: UBT

text: public

text: temperate biome [ENVO_01001831]

text: 16S rRNA V4 reads from the proximal rhizosphere of Zea mays L. grown at an agricultural field in Ruhstorf, Germany. This sample was taken at the flowering plant developmental stage and subjected to natural precipitation reduced by 60%. Maize variety: Gelber Badischer Landmais.

text: 367 m

text: rhizosphere environment [ENVO_01000999]

text: Germany

text: Bavaria, Lower Bavaria, Pocking

text: 67 d

text: maize

text: flowering

text: maize field [ENVO_01000348]

text: Extraction with water instead of sample and PCR blank control.

text: soil metagenome

text: initial denaturation:98degC_120s; denaturation:98degC_10s; annealing:55degC_30s; extension:72degC_30s; final extension:72degC_120s

text: not applicable

text: Rhizo4Bio: RhizoTraits

text: natural precipitation reduced by 60%

text: -80 degC

text: soil metagenome

text: Illumina iSeq 100

text: variety:Gelber Badischer Landmais

text: 16S rRNA


text: UBT

text: public

text: temperate biome [ENVO_01001831]

text: 16S rRNA V4 reads from bulk soil at an agricultural field in Ruhstorf, Germany. This sample was taken at the grain filling plant developmental stage and subjected to natural precipitation reduced by 60%. Maize variety: Gelber Badischer Landmais.

text: 367 m

text: bulk soil [ENVO_00005802]

text: Germany

text: Bavaria, Lower Bavaria, Pocking

text: 95 d

text: maize

text: grain filling

text: maize field [ENVO_01000348]

text: soil metagenome

text: 515f: 5'-GTGYCAGCMGCCGCGGTAA-3', 806RB: 5'-GGACTACNVGGGTWTCTAAT-3'

text: not applicable

text: Bacterial mock community of the following taxa: Bacillus subtilis (NCBI:txid1423), Chryseobacterium luteum, Clostridium roseum (NCBI:txid421531), Enterobacter mori (NCBI:txid539813), Franconibacter helveticus (NCBI:txid357240), Glycomyces tenuis (NCBI:txid58116), Pseudomonas fluorescens (NCBI:txid294), Pseudomonas putida (NCBI:txid303), Rhizobium endophyticum (NCBI:txid578290), Streptomyces acidiscabies (NCBI:txid42234), Variovorax guangxiensis (NCBI:txid1775474). Amplicons were generated individually for each taxon with the same conditions as for the other samples and pooled in equimolar manner after amplification.

text: Rhizo4Bio: RhizoTraits

text: natural precipitation reduced by 60%

text: -80 degC

text: soil metagenome

text: Illumina iSeq 100

text: variety:Gelber Badischer Landmais

text: 16S rRNA

text: prokaryotic microbiome in bulk soil


text: UBT

text: public

text: temperate biome [ENVO_01001831]

text: 16S rRNA V4 reads from roots (rhizoplane and endosphere) of Zea mays L. grown at an agricultural field in Ruhstorf, Germany. This sample was taken at the grain filling plant developmental stage and subjected to natural precipitation reduced by 60%. Maize variety: Gelber Badischer Landmais.

text: 367 m

text: root [PO_0009005]

text: Germany

text: Bavaria, Lower Bavaria, Pocking

text: 95 d

text: maize

text: grain filling

text: maize field [ENVO_01000348]

text: root metagenome

text: Rhizo4Bio: RhizoTraits

text: natural precipitation reduced by 60%

text: -80 degC

text: root metagenome

text: Illumina iSeq 100

text: variety:Gelber Badischer Landmais

text: 16S rRNA

text: prokaryotic microbiome in roots of Zea mays L.


text: UBT

text: public

text: temperate biome [ENVO_01001831]

text: 16S rRNA V4 reads from the proximal rhizosphere of Zea mays L. grown at an agricultural field in Ruhstorf, Germany. This sample was taken at the grain filling plant developmental stage and subjected to natural precipitation reduced by 60%. Maize variety: Gelber Badischer Landmais.

text: 367 m

text: rhizosphere environment [ENVO_01000999]

text: Germany

text: Bavaria, Lower Bavaria, Pocking

text: 95 d

text: maize

text: grain filling

text: maize field [ENVO_01000348]

text: soil metagenome

text: not applicable

text: Rhizo4Bio: RhizoTraits

text: natural precipitation reduced by 60%

text: -80 degC

text: soil metagenome

text: Illumina iSeq 100

text: variety:Gelber Badischer Landmais

text: 16S rRNA


text: UBT

text: public

text: temperate biome [ENVO_01001831]

text: 16S rRNA V4 reads from the distal rhizosphere of Zea mays L. grown at an agricultural field in Ruhstorf, Germany. This sample was taken at the grain filling plant developmental stage and subjected to natural precipitation reduced by 60%. Maize variety: Gelber Badischer Landmais.

text: 367 m

text: rhizosphere environment [ENVO_01000999]

text: Germany

text: Bavaria, Lower Bavaria, Pocking

text: 95 d

text: maize

text: grain filling

text: maize field [ENVO_01000348]

text: soil metagenome

text: not applicable

text: Rhizo4Bio: RhizoTraits

text: natural precipitation reduced by 60%

text: -80 degC

text: soil metagenome

text: Illumina iSeq 100

text: variety:Gelber Badischer Landmais

text: 16S rRNA


text: UBT

text: public

text: temperate biome [ENVO_01001831]

text: 16S rRNA V4 reads from bulk soil at an agricultural field in Ruhstorf, Germany. This sample was taken at the flowering plant developmental stage and subjected to natural precipitation reduced by 60%. Maize variety: Braunes Schindelmeiser.

text: 367 m

text: bulk soil [ENVO_00005802]

text: Germany

text: Bavaria, Lower Bavaria, Pocking

text: 61 d

text: maize

text: flowering

text: maize field [ENVO_01000348]

text: soil metagenome

text: not applicable

text: Rhizo4Bio: RhizoTraits

text: natural precipitation reduced by 60%

text: -80 degC

text: soil metagenome

text: Illumina iSeq 100

text: variety:Braunes Schindelmeiser

text: 16S rRNA

text: prokaryotic microbiome in bulk soil


text: UBT

text: public

text: temperate biome [ENVO_01001831]

text: 16S rRNA V4 reads from roots (rhizoplane and endosphere) of Zea mays L. grown at an agricultural field in Ruhstorf, Germany. This sample was taken at the flowering plant developmental stage and subjected to natural precipitation reduced by 60%. Maize variety: Braunes Schindelmeiser.

text: 367 m

text: root [PO_0009005]

text: Germany

text: Bavaria, Lower Bavaria, Pocking

text: 61 d

text: maize

text: flowering

text: maize field [ENVO_01000348]

text: root metagenome

text: Rhizo4Bio: RhizoTraits

text: natural precipitation reduced by 60%

text: -80 degC

text: root metagenome

text: Illumina iSeq 100

text: variety:Braunes Schindelmeiser

text: 16S rRNA


text: UBT

text: public

text: temperate biome [ENVO_01001831]

text: 16S rRNA V4 reads from the proximal rhizosphere of Zea mays L. grown at an agricultural field in Ruhstorf, Germany. This sample was taken at the flowering plant developmental stage and subjected to natural precipitation reduced by 60%. Maize variety: Braunes Schindelmeiser.

text: 367 m

text: rhizosphere environment [ENVO_01000999]

text: Germany

text: Bavaria, Lower Bavaria, Pocking

text: 61 d

text: maize

text: flowering

text: maize field [ENVO_01000348]

text: soil metagenome

text: not applicable

text: Rhizo4Bio: RhizoTraits

text: natural precipitation reduced by 60%

text: -80 degC

text: soil metagenome

text: Illumina iSeq 100

text: variety:Braunes Schindelmeiser

text: 16S rRNA

text: prokaryotic microbiome in the proximal rhizosphere of Zea mays L.


text: UBT

text: public

text: temperate biome [ENVO_01001831]

text: 16S rRNA V4 reads from bulk soil at an agricultural field in Ruhstorf, Germany. This sample was taken at the grain filling plant developmental stage and subjected to natural precipitation reduced by 60%. Maize variety: Braunes Schindelmeiser.

text: 367 m

text: bulk soil [ENVO_00005802]

text: YaraBela SULFAN (YARA GmbH & Co. KG, D�lmen, Germany)

text: Germany

text: Bavaria, Lower Bavaria, Pocking

text: 93 d

text: maize

text: grain filling

text: maize field [ENVO_01000348]

text: soil metagenome

text: not applicable

text: Rhizo4Bio: RhizoTraits

text: natural precipitation reduced by 60%

text: -80 degC

text: soil metagenome

text: Illumina iSeq 100

text: variety:Braunes Schindelmeiser

text: 16S rRNA

text: prokaryotic microbiome in bulk soil


text: UBT

text: public

text: temperate biome [ENVO_01001831]

text: 16S rRNA V4 reads from roots (rhizoplane and endosphere) of Zea mays L. grown at an agricultural field in Ruhstorf, Germany. This sample was taken at the grain filling plant developmental stage and subjected to natural precipitation reduced by 60%. Maize variety: Braunes Schindelmeiser.

text: 367 m

text: root [PO_0009005]

text: Germany

text: Bavaria, Lower Bavaria, Pocking

text: Bromotril 225EC (ADAMA Agricultural Solutions, Ashdod, Israel), MaisTer power (Bayer CropScience Deutschland GmbH, Monheim, Germany)

text: 93 d

text: maize

text: grain filling

text: maize field [ENVO_01000348]

text: root metagenome

text: Rhizo4Bio: RhizoTraits

text: natural precipitation reduced by 60%

text: -80 degC

text: root metagenome

text: Illumina iSeq 100

text: variety:Braunes Schindelmeiser

text: 16S rRNA


text: UBT

text: public

text: temperate biome [ENVO_01001831]

text: 16S rRNA V4 reads from the proximal rhizosphere of Zea mays L. grown at an agricultural field in Ruhstorf, Germany. This sample was taken at the grain filling plant developmental stage and subjected to natural precipitation reduced by 60%. Maize variety: Braunes Schindelmeiser.

text: 367 m

text: rhizosphere environment [ENVO_01000999]

text: Germany

text: Bavaria, Lower Bavaria, Pocking

text: 93 d

text: maize

text: grain filling

text: maize field [ENVO_01000348]

text: Extraction with water instead of sample and PCR blank control.

text: soil metagenome

text: not applicable

text: Rhizo4Bio: RhizoTraits

text: natural precipitation reduced by 60%

text: -80 degC

text: soil metagenome

text: Illumina iSeq 100

text: variety:Braunes Schindelmeiser

text: 16S rRNA


text: UBT

text: public

text: temperate biome [ENVO_01001831]

text: 16S rRNA V4 reads from the distal rhizosphere of Zea mays L. grown at an agricultural field in Ruhstorf, Germany. This sample was taken at the grain filling plant developmental stage and subjected to natural precipitation reduced by 60%. Maize variety: Braunes Schindelmeiser.

text: 367 m

text: rhizosphere environment [ENVO_01000999]

text: Germany

text: Bavaria, Lower Bavaria, Pocking

text: 93 d

text: maize

text: grain filling

text: maize field [ENVO_01000348]

text: soil metagenome

text: initial denaturation:98degC_120s; denaturation:98degC_10s; annealing:55degC_30s; extension:72degC_30s; final extension:72degC_120s

text: not applicable

text: Rhizo4Bio: RhizoTraits

text: natural precipitation reduced by 60%

text: -80 degC

text: soil metagenome

text: Illumina iSeq 100

text: variety:Braunes Schindelmeiser

text: 16S rRNA

text: prokaryotic microbiome in the distal rhizosphere of Zea mays L.


text: UBT

text: public

text: missing: control sample

text: missing: control sample

text: Blank control extracted with water instead of sample. Treated like the other samples in the subsequent steps.

text: missing: control sample

text: missing: control sample

text: missing: control sample

text: missing: control sample

text: missing: control sample

text: missing: control sample

text: missing: control sample

text: missing: control sample

text: missing: control sample

text: missing: control sample

text: missing: control sample

text: missing: control sample

text: missing: control sample

text: blank sample

text: 515f: 5'-GTGYCAGCMGCCGCGGTAA-3', 806RB: 5'-GGACTACNVGGGTWTCTAAT-3'

text: missing: control sample

text: Bacterial mock community of the following taxa: Bacillus subtilis (NCBI:txid1423), Chryseobacterium luteum, Clostridium roseum (NCBI:txid421531), Enterobacter mori (NCBI:txid539813), Franconibacter helveticus (NCBI:txid357240), Glycomyces tenuis (NCBI:txid58116), Pseudomonas fluorescens (NCBI:txid294), Pseudomonas putida (NCBI:txid303), Rhizobium endophyticum (NCBI:txid578290), Streptomyces acidiscabies (NCBI:txid42234), Variovorax guangxiensis (NCBI:txid1775474). Amplicons were generated individually for each taxon with the same conditions as for the other samples and pooled in equimolar manner after amplification.

text: Rhizo4Bio: RhizoTraits

text: missing: control sample

text: missing: control sample

text: blank sample

text: Illumina iSeq 100

text: missing: control sample

text: 16S rRNA

text: blank control


text: UBT

text: public

text: missing: control sample

text: missing: control sample

text: PCR blank control with water instead of DNA extract. Treated like the other samples in the subsequent steps.

text: missing: control sample

text: missing: control sample

text: missing: control sample

text: missing: control sample

text: missing: control sample

text: missing: control sample

text: missing: control sample

text: missing: control sample

text: missing: control sample

text: missing: control sample

text: missing: control sample

text: missing: control sample

text: missing: control sample

text: blank sample

text: missing: control sample

text: Rhizo4Bio: RhizoTraits

text: missing: control sample

text: missing: control sample

text: blank sample

text: Illumina iSeq 100

text: missing: control sample

text: 16S rRNA

text: PCR blank control


text: UBT

text: public

text: missing: control sample

text: missing: control sample

text: missing: control sample

text: missing: control sample

text: missing: control sample

text: missing: control sample

text: missing: control sample

text: missing: control sample

text: missing: control sample

text: missing: control sample

text: missing: control sample

text: missing: control sample

text: missing: control sample

text: missing: control sample

text: missing: control sample

text: mixed sample

text: missing: control sample

text: Rhizo4Bio: RhizoTraits

text: missing: control sample

text: missing: control sample

text: mixed sample

text: Illumina iSeq 100

text: missing: control sample

text: 16S rRNA

text: mock community


text: INRAE

text: public

text: Ovary from control chicken

text: Growing

text: France

text: Gallus gallus gallus

text: Gallus gallus gallus

text: Female

characteristic: sex

text: ovary

text: Control ovary

text: Ross 308

characteristic: variety


text: INRAE

text: public

text: Ovary from chicken dietary exposed to Glyphosate Based Herbicide (GBH) at low concentration (13 mg eq Glyphosate/kg body weight/day

text: Growing

text: France

text: Gallus gallus gallus

text: Gallus gallus gallus

text: Female

text: ovary

text: GBH- ovary

text: Ross 308


text: INRAE

text: public

text: Ovary from chicken dietary exposed to Glyphosate Based Herbicide (GBH) at high concentration (34 mg eq Glyphosate/kg body weight/day

text: Growing

text: France

text: Gallus gallus gallus

text: Gallus gallus gallus

text: Female

text: ovary

text: GBH+ ovary

text: Ross 308


text: WDRC KAUST

text: public

text: Influent sample

text: Saudi Arabia

text: metagenome

characteristic: investigation type

text: wastewater metagenome

text: wastewater metagenome

text: Municipal wastewater treatment plant

characteristic: sewage type


text: WDRC KAUST

text: public

text: Sludge sample

text: Saudi Arabia

text: metagenome

text: wastewater metagenome

text: wastewater metagenome

text: Municipal wastewater treatment plant


text: WDRC KAUST

text: public

text: Effluent sample

text: Saudi Arabia

text: metagenome

text: wastewater metagenome

text: wastewater metagenome

text: Municipal wastewater treatment plant


text: Department of Zoology

text: public

text: 16S amplicones

text: Czech Republic

text: metagenome

text: metagenome

text: House mouse microbiota


text: NGI

text: public

text: human

characteristic: common name

text: Promega CAT#G1471

text: Sweden

text: Homo sapiens

text: Homo sapiens


characteristic: ENA first public

text: CDA

text: public

text: not provided

text: bread wheat

characteristic: cultivar

text: Total RNA extracted from leaf tissue of Triticum aestivum, Mutant_SrA_12a

text: not provided

text: Triticum aestivum

text: Triticum aestivum

text: Leaf

text: Zahir-1644 Wheat Leaf RNA_4


characteristic: ENA-CHECKLIST

text: CDA

characteristic: INSDC center name

text: public

text: not provided

text: bread wheat

text: Total RNA extracted from leaf tissue of Triticum aestivum, Mutant_SrA_263g

text: not provided

text: Triticum aestivum

text: Triticum aestivum

text: Leaf

text: Zahir-1644 Wheat Leaf RNA_5


text: CDA

text: public

characteristic: INSDC status

characteristic: SRA accession

text: not provided

text: bread wheat

text: Total RNA extracted from leaf tissue of Triticum aestivum, Mutant_SrA_267d

text: not provided

text: Triticum aestivum

text: Triticum aestivum

text: Leaf

text: Zahir-1644 Wheat Leaf RNA_6


text: CDA

text: public

characteristic: Submitter Id

text: not provided

characteristic: collection date

text: bread wheat

text: Total RNA extracted from leaf tissue of Triticum aestivum, Mutant_SrA_44d

text: not provided

text: Triticum aestivum

text: Triticum aestivum

text: Leaf

text: Zahir-1644 Wheat Leaf RNA_7


text: CDA

text: public

text: not provided

text: bread wheat

text: Total RNA extracted from leaf tissue of Triticum aestivum, Mutant_SrA_190d

characteristic: description

text: not provided

text: Triticum aestivum

text: Triticum aestivum

text: Leaf

text: Zahir-1644 Wheat Leaf RNA_9


text: FRIGE

text: public

text: human

text: Patient

text: India

characteristic: geographic location (country and/or sea)

text: Homo sapiens

text: Homo sapiens


text: Earth Hologenome Iniatiative

text: public

text: 1000218 - Xeric shrubland

characteristic: broad-scale environmental context

text: host

characteristic: environmental medium

text: Australia

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Faecal

characteristic: host body site

text: Southern hairy-nosed wombat

characteristic: host common name

text: Adult

characteristic: host life stage

text: Lasiorhinus latifrons

characteristic: host scientific name

text: female

characteristic: host sex

text: 1000218 - Xeric shrubland

characteristic: local environmental context

text: marsupial metagenome

characteristic: organism

characteristic: project name

text: marsupial metagenome


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Earth Hologenome Iniatiative

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 1000218 - Xeric shrubland

text: Pouch: Very moist, Dirty, Deep

text: host

text: Australia

text: Pouch

text: Southern hairy-nosed wombat

text: Adult

text: Lasiorhinus latifrons

text: female

text: 1000218 - Xeric shrubland

text: marsupial metagenome

text: marsupial metagenome

characteristic: title


text: Earth Hologenome Iniatiative

text: public

characteristic: Submitter Id

text: 1000218 - Xeric shrubland

characteristic: broad-scale environmental context

characteristic: collection date

text: Pouch: Very moist, dark red, dirty, deep

characteristic: description

text: host

characteristic: environmental medium

text: Australia

characteristic: geographic location (country and/or sea)

text: Pouch

text: Southern hairy-nosed wombat

text: Adult

text: Lasiorhinus latifrons

text: female

text: 1000218 - Xeric shrubland

text: marsupial metagenome

text: marsupial metagenome


text: Earth Hologenome Iniatiative

text: public

text: 1000218 - Xeric shrubland

text: Pouch: Late lactation

text: host

text: Australia

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Pouch

characteristic: host body site

text: Southern hairy-nosed wombat

characteristic: host common name

text: Adult

characteristic: host life stage

text: Lasiorhinus latifrons

characteristic: host scientific name

text: female

text: 1000218 - Xeric shrubland

text: marsupial metagenome

text: marsupial metagenome


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Earth Hologenome Iniatiative

text: public

text: 1000218 - Xeric shrubland

text: Pouch: Moist, pink, dirty, deep, Pouch young roughly 8 weeks

text: host

text: Australia

text: Pouch

text: Southern hairy-nosed wombat

text: Adult

text: Lasiorhinus latifrons

text: female

characteristic: host sex

text: 1000218 - Xeric shrubland

characteristic: local environmental context

text: marsupial metagenome

characteristic: organism

characteristic: project name

text: marsupial metagenome


text: Earth Hologenome Iniatiative

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 1000218 - Xeric shrubland

characteristic: broad-scale environmental context

text: Pouch: Dry and shallow

text: host

text: Australia

text: Pouch

text: Southern hairy-nosed wombat

text: Adult

text: Lasiorhinus latifrons

text: female

text: 1000218 - Xeric shrubland

text: marsupial metagenome

text: marsupial metagenome

characteristic: title


text: Earth Hologenome Iniatiative

text: public

text: 1000218 - Xeric shrubland

characteristic: collection date

text: same individual as 207_PS. Pouch: Anoestrus

characteristic: description

text: host

characteristic: environmental medium

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Pouch

text: Southern hairy-nosed wombat

text: Adult

text: Lasiorhinus latifrons

text: female

text: 1000218 - Xeric shrubland

text: marsupial metagenome

text: marsupial metagenome


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Earth Hologenome Iniatiative

text: public

text: 1000218 - Xeric shrubland

text: joey 475g

text: host

text: Australia

text: Faecal

characteristic: host body site

text: Southern hairy-nosed wombat

characteristic: host common name

text: Adult

characteristic: host life stage

text: Lasiorhinus latifrons

characteristic: host scientific name

text: female

text: 1000218 - Xeric shrubland

text: marsupial metagenome

text: marsupial metagenome


text: Earth Hologenome Iniatiative

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: 1000218 - Xeric shrubland

text: joey 475g

text: host

text: Australia

text: Skin

text: Southern hairy-nosed wombat

text: Juvenile

text: Lasiorhinus latifrons

text: female

characteristic: host sex

text: 1000218 - Xeric shrubland

characteristic: local environmental context

text: marsupial metagenome

characteristic: organism

characteristic: project name

text: marsupial metagenome


text: Earth Hologenome Iniatiative

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: 1000218 - Xeric shrubland

characteristic: broad-scale environmental context

characteristic: collection date

text: joey 475g

characteristic: description

text: host

text: Australia

text: Pouch

text: Southern hairy-nosed wombat

text: Adult

text: Lasiorhinus latifrons

text: female

text: 1000218 - Xeric shrubland

text: marsupial metagenome

text: marsupial metagenome

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Earth Hologenome Iniatiative

text: public

text: 1000218 - Xeric shrubland

text: joey 16g. same individual as EHI01380

text: host

characteristic: environmental medium

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Pouch

text: Southern hairy-nosed wombat

text: Adult

text: Lasiorhinus latifrons

text: female

text: 1000218 - Xeric shrubland

text: marsupial metagenome

text: marsupial metagenome


text: Earth Hologenome Iniatiative

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: 1000218 - Xeric shrubland

text: joey 39g

text: host

text: Australia

text: Pouch

characteristic: host body site

text: Southern hairy-nosed wombat

characteristic: host common name

text: Adult

characteristic: host life stage

text: Lasiorhinus latifrons

characteristic: host scientific name

text: female

text: 1000218 - Xeric shrubland

text: marsupial metagenome

text: marsupial metagenome


text: Imagine Institute

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: human

characteristic: common name

text: France

text: Homo sapiens

characteristic: organism

text: Homo sapiens


text: Wellcome Sanger Institute

text: public

text: ArrayExpress

characteristic: broker name

text: human induced pluripotent stem cells

text: not collected

characteristic: collection date

text: human

text: Protocols: For dissociation of organoids on day −7, they were removed from culture insert and incubated in digestion buffer, which consisted of collagenase type IV solution (StemCell Technologies) supplemented with 0.88mg/ml collagenase/dispase (Roche) and 50U DNase I (Sigma), for 20 minutes at 37oC. Vigorous pipetting was performed in the middle of the incubation and at the end. After complete disaggregation, single cell suspension was prepared by passing through a 50-μm strainer.  For dissociation of organoids from day 0 onwards, a cell scraper was used to detach ATOs from cell culture insert membranes and detached ATOs were then submerged in cold flow buffer (PBS (Gibco) containing 2% (v/v) fetal bovine serum (FBS; Gibco) and 2 mM EDTA (Invitrogen)). Culture inserts were washed and detached ATOs were pipetted up and down to form single-cell suspension before passing through a 50-μm strainer.   Cells were then stained with designed panels of antibodies and analyzed by flow cytometry. FACS was performed at the same time and live human DAPI–anti-mouse CD29– (APC/Cy7 anti-mouse CD29 antibody, Biolegend, 102225) cells were sorted for day −7, day 0 and week 3 samples, and live (DAPI–) cells were sorted for week 5 and week 7 samples before loading onto each channel of the Chromium chip from Chromium single cell V(D)J kit (10X Genomics). The metadata for all the ATO samples can be found in ATO_manifest.csv. For the day −14 sample, some sorted (both hEMP and the rest of DAPI– fraction) and unsorted cells were stained with hashtag antibodies (TotoalSeq-C antibodies from Biolegend, see ATO_hashtagging.csv, following 10X cell surface protein labeling protocol) before being mixed together with some mouse stromal cells (MS5-hDLL4) for 10X loading. For week 1 sample, hashtag antibodies were added in at the same time as the FACS antibodies i.e. before sorting.  We followed the PSC-ATO protocol as previously described30. iPSC cells were harvested as a single-cell suspension and seeded (3×106 cells per well) in GFR reduced Matrigel (Corning) - coated 6-well plates in X-VIVO 15 media (Lonza), supplemented with rhActivin A, rhBMP4, rhVEGF, rhFGF (all from R&D Systems), and ROCK inhibitor (Y27632; LKT Labs) on day −17, and only rhBMP4, rhVEGF and rhFGF on days −16 and −15. Cells were harvested 3.5 days later (day−14) , and isolated by FACS for CD326–CD56+ (PE anti-human CD326 antibody, Biolegend, 324205; APC anti-human CD56 antibody, Biolegend, 318309) human embryonic mesodermal progenitors (hEMPs).  Isolated hEMPs were combined with MS5-hDLL4 at a ratio of 1:50. Two or three cell-dense droplets (5×105 cells in 6 μl hematopoietic induction medium) were deposited on top of an insert in each well of a six-well plate. Hematopoietic induction medium composed of EGM2 (Lonza) supplemented with ROCK inhibitor and SB blocker (TGF-β receptor kinase inhibitor SB- 431542; Abcam) was added into the wells outside the inserts so that the cells sat at the air-liquid interface. The organoids were then cultured in EGM2 with SB blocker for 7 days (days −14 to −7), before the addition of cytokines rhSCF, rhFLT3L, rhTPO (all from Peprotech) between days −6 to 0. These 2 weeks formed the hematopoietic induction phase. On day 1, media was changed again to RB27 (RPMI supplemented with B27 (Gibco), ascorbic acid (Sigma-Aldrich), penicillin/streptomycin (Sigma-Aldrich) and glutamax (Thermo Fisher Scientific)) with rhSCF, rhFLT3L and rhIL7. The organoids can be maintained in culture for 7 more weeks in this medium.  For the TNFɑ validation experiment, we followed the PSC-ATO protocol described previously using Kolf iPSC line. One plate (12 organoids) was set for each condition, with 4 conditions which were 1) control i.e., no TNFɑ addition, 2) 1 ng/ml TNFɑ (final  concentration), 3) 5 ng/ml TNFɑ, and 4) 25 ng/ml TNFɑ. TNFɑ was added into the media between week 6 to 7. Organoids were harvested at week 7, stained with hashtag antibodies (TotalSeq-C antibodies from Biolegend) and a designed panel of FACS antibodies, and sorted for CD45+ cells before 10X loading.  Single-cell cDNA synthesis, amplification and gene expression (GEX) and cell surface protein (CITE-seq) libraries were generated following the manufacturer’s instructions.  Single-cell cDNA synthesis, amplification and gene expression (GEX) and cell surface protein (CITE-seq) libraries were generated following the manufacturer’s instructions.

characteristic: description

text: not collected

characteristic: growth condition

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Genomes for Australian Plants

text: public

text: ena-SAMPLE-TAB-29-02-2024-03:11:52:076-30318

text: Not recorded

text: Grastidium baileyi (F.Muell.) Rauschert, Orchidaceae, CANB 507319

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: wild collected

characteristic: isolation and growth condition

text: Dendrobium baileyi

text: Adult

characteristic: plant developmental stage

text: wild collected

characteristic: plant growth medium

text: Leaf tissue

characteristic: plant structure

text: Dendrobium baileyi

text: CANB 507319

characteristic: source material identifiers

text: Target capture data for Grastidium baileyi (F.Muell.) Rauschert, Bioplatforms Australia Sample ID: 102.100.100/79953 using Angiosperms353 bait kit 10.1093/sysbio/syy086


text: Genomes for Australian Plants

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-29-02-2024-03:11:52:076-30319

characteristic: Submitter Id

text: Not recorded

characteristic: common name

text: Drabastrum alpestre (F.Muell.) O.E.Schulz, Brassicaceae, CANB 504902

text: Australia

text: wild collected

text: Drabastrum alpestre

characteristic: organism

text: Adult

text: wild collected

text: Leaf tissue

text: Drabastrum alpestre

text: CANB 504902

text: Target capture data for Drabastrum alpestre (F.Muell.) O.E.Schulz, Bioplatforms Australia Sample ID: 102.100.100/80168 using Angiosperms353 bait kit 10.1093/sysbio/syy086


text: Genomes for Australian Plants

text: public

text: ena-SAMPLE-TAB-29-02-2024-03:11:52:076-30320

characteristic: collection date

text: Not recorded

text: Potamophila parviflora R.Br., Poaceae, CANB 489379

characteristic: description

text: Australia

text: wild collected

text: Potamophila parviflora

text: Adult

text: wild collected

text: Leaf tissue

text: Potamophila parviflora

text: CANB 489379

text: Target capture data for Potamophila parviflora R.Br., Bioplatforms Australia Sample ID: 102.100.100/80279 using Angiosperms353 bait kit 10.1093/sysbio/syy086

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Genomes for Australian Plants

text: public

text: ena-SAMPLE-TAB-29-02-2024-03:11:52:076-30321

text: Not recorded

text: Levieria acuminata (F.Muell.) Perkins, Monimiaceae, NSW933451

text: Australia

characteristic: geographic location (country and/or sea)

text: wild collected

text: Levieria acuminata

text: Adult

text: wild collected

text: Leaf tissue

text: Levieria acuminata

text: Target capture data for Levieria acuminata (F.Muell.) Perkins, Bioplatforms Australia Sample ID: 102.100.100/80394 using Angiosperms353 bait kit 10.1093/sysbio/syy086


text: Genomes for Australian Plants

text: public

text: ena-SAMPLE-TAB-29-02-2024-03:11:52:076-30322

text: Not recorded

text: Palmeria scandens F.Muell., Monimiaceae, NSW845956

text: Australia

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: wild collected

characteristic: isolation and growth condition

text: Palmeria scandens

text: Adult

text: wild collected

text: Leaf tissue

text: Palmeria scandens

text: Target capture data for Palmeria scandens F.Muell., Bioplatforms Australia Sample ID: 102.100.100/80395 using Angiosperms353 bait kit 10.1093/sysbio/syy086


text: Genomes for Australian Plants

text: public

text: ena-SAMPLE-TAB-29-02-2024-03:11:52:076-30323

text: Not recorded

text: Neosepicaea jucunda (F.Muell.) Steenis, Bignoniaceae, CNS 138541

text: Australia

text: wild collected

text: Neosepicaea jucunda

text: Adult

characteristic: plant developmental stage

text: wild collected

characteristic: plant growth medium

text: Leaf tissue

characteristic: plant structure

text: Neosepicaea jucunda

text: CNS 138541

text: Target capture data for Neosepicaea jucunda (F.Muell.) Steenis, Bioplatforms Australia Sample ID: 102.100.100/80935 using Angiosperms353 bait kit 10.1093/sysbio/syy086


text: Genomes for Australian Plants

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-29-02-2024-03:11:52:076-30324

text: Not recorded

text: Gymnostoma australianum L.A.S.Johnson, Casuarinaceae, CNS 129655

text: Australia

text: wild collected

text: Gymnostoma australianum

text: Adult

text: wild collected

text: Leaf tissue

text: Gymnostoma australianum

text: CNS 129655

characteristic: source material identifiers

text: Target capture data for Gymnostoma australianum L.A.S.Johnson, Bioplatforms Australia Sample ID: 102.100.100/80936 using Angiosperms353 bait kit 10.1093/sysbio/syy086


text: Genomes for Australian Plants

text: public

characteristic: SRA accession

text: ena-SAMPLE-TAB-29-02-2024-03:11:52:076-30325

characteristic: Submitter Id

text: Not recorded

characteristic: common name

text: Arthragrostis brassiana var. minutiflora B.K.Simon, Poaceae, BRI AQ0914285

text: Australia

text: wild collected

text: Panicum brassianum

text: Adult

text: wild collected

text: Leaf tissue

text: Panicum brassianum

text: BRI AQ0914285

text: Target capture data for Arthragrostis brassiana var. minutiflora B.K.Simon, Bioplatforms Australia Sample ID: 102.100.100/81006 using Angiosperms353 bait kit 10.1093/sysbio/syy086


text: Genomes for Australian Plants

text: public

text: ena-SAMPLE-TAB-29-02-2024-03:11:52:076-30326

characteristic: collection date

text: Not recorded

text: x Cynochloris reynoldensis B.K.Simon, Poaceae, BRI AQ0424737

characteristic: description

text: Australia

text: wild collected

text: x Cynochloris reynoldensis

characteristic: organism

text: Adult

text: wild collected

text: Leaf tissue

text: x Cynochloris reynoldensis

text: BRI AQ0424737

text: Target capture data for x Cynochloris reynoldensis B.K.Simon, Bioplatforms Australia Sample ID: 102.100.100/81105 using Angiosperms353 bait kit 10.1093/sysbio/syy086


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Genomes for Australian Plants

text: public

text: ena-SAMPLE-TAB-29-02-2024-03:11:52:076-30327

text: Not recorded

text: Dallwatsonia felliana B.K.Simon, Poaceae, BRI AQ0914940

text: Australia

text: wild collected

text: Hymenachne felliana

text: Adult

text: wild collected

text: Leaf tissue

text: Hymenachne felliana

text: BRI AQ0914940

text: Target capture data for Dallwatsonia felliana B.K.Simon, Bioplatforms Australia Sample ID: 102.100.100/81150 using Angiosperms353 bait kit 10.1093/sysbio/syy086

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of East Anglia

characteristic: INSDC first public

characteristic: INSDC last update

text: public

tag: Namespace:University of East Anglia

text: National Institute for Communicable Disease, South Africa

text: Salmonella Infantis strain isolated from a human in South Africa

text: South Africa

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

characteristic: isolate

text: Human Stool

text: Salmonella enterica subsp. enterica

text: Salmonella enterica subsp. enterica

text: Infantis

characteristic: serovar

text: enterica


text: IBENS

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: thale cress

characteristic: common name

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: Fast co-evolution of anti-silencing systems and target sequences shapes the invasiveness of Mu-like DNA transposons


text: medium

characteristic: Compound

text: 0 µg/ml

characteristic: Concentration

characteristic: ENA-CHECKLIST

text: 24 h

characteristic: Exposure time

text: female

characteristic: Gender

text: University of Konstanz

text: public

tag: Namespace:University of Konstanz

characteristic: Treatment

text: once

characteristic: Treatment scheme

text: EU-ToxRisk DCC

characteristic: broker name

text: human immature dorsal root ganglia neurons

text: human

text: Homo sapiens

characteristic: organism

text: Homo sapiens


text: Dox

text: 2 µg/ml

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: 24 h

characteristic: External Id

text: female

text: University of Konstanz

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: once

text: EU-ToxRisk DCC

text: human

text: Homo sapiens

text: Homo sapiens


text: Matsapume

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: bare sand

characteristic: description

text: water

characteristic: environment (biome)

text: water

characteristic: environment (feature)

text: water ERC000024

text: Thailand

characteristic: geographic location (country and/or sea)

unit: m

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: aquatic metagenome

characteristic: project name

text: aquatic metagenome

text: Illumina

characteristic: sequencing method

characteristic: title

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Matsapume

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: Sand from healthy sea cucumber

text: water

text: water

text: water ERC000024

text: Thailand

text: metagenome

text: aquatic metagenome

characteristic: organism

text: aquatic metagenome

text: Illumina

text: water


text: MAQC

characteristic: Compound

text: nan µM

characteristic: Concentration

text: nan h

characteristic: Exposure time

text: female

characteristic: Gender

text: Stockholm University

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Stockholm University

text: single dose

characteristic: Treatment

text: once

characteristic: Treatment scheme

text: EU-ToxRisk DCC

characteristic: broker name

text: human

characteristic: common name

text: Homo sapiens

text: Homo sapiens


text: nan µM

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: 2.0 h

text: female

text: Stockholm University

text: public

text: single dose

text: once

text: EU-ToxRisk DCC

text: human

text: Homo sapiens

text: Homo sapiens


text: nan µM

text: 6.0 h

characteristic: External Id

text: female

text: Stockholm University

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: single dose

text: once

text: EU-ToxRisk DCC

text: human

text: Homo sapiens

text: Homo sapiens


text: Nicotine

characteristic: Compound

text: 1 µM

characteristic: Concentration

text: 2.0 h

text: female

text: Stockholm University

text: public

text: single dose

text: once

text: EU-ToxRisk DCC

text: human

text: Homo sapiens

characteristic: organism

text: Homo sapiens


text: Nicotine

text: 1 µM

text: 6.0 h

characteristic: Exposure time

text: female

characteristic: Gender

text: Stockholm University

characteristic: INSDC center name

text: public

text: single dose

text: once

text: EU-ToxRisk DCC

text: human

text: Homo sapiens

text: Homo sapiens


text: Nicontine+PNU

text: 1+10 µM

text: 2.0 h

text: female

text: Stockholm University

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: single dose

text: once

text: EU-ToxRisk DCC

text: human

text: Homo sapiens

text: Homo sapiens


text: Nicontine+PNU

text: 1+10 µM

text: 6.0 h

text: female

text: Stockholm University

text: public

text: single dose

characteristic: Treatment

text: once

characteristic: Treatment scheme

text: EU-ToxRisk DCC

characteristic: broker name

text: human

text: Homo sapiens

text: Homo sapiens


text: Nicotine+PNU

text: 1+10 µM

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: 2.0 h

text: female

text: Stockholm University

text: public

text: single dose

text: once

text: EU-ToxRisk DCC

text: human

characteristic: common name

text: Homo sapiens

text: Homo sapiens


text: Nicotine+PNU

text: 1+10 µM

characteristic: ENA-LAST-UPDATE

text: 6.0 h

characteristic: External Id

text: female

text: Stockholm University

characteristic: INSDC first public

text: public

text: single dose

text: once

text: EU-ToxRisk DCC

text: human

text: Homo sapiens

text: Homo sapiens


text: ROBERT KOCH INSTITUT

text: public

tag: Namespace:ROBERT KOCH INSTITUT

characteristic: Submitter Id

text: RespiCoV

characteristic: description

text: Severe acute respiratory syndrome-related coronavirus

text: RespiCoV

characteristic: title


text: public

tag: Namespace:European Research Institute for the Biology of Ageing University Medical Center Groningen

unit: month

characteristic: age

text: ArrayExpress

text: tumor single-cell suspension

text: house mouse

text: Protocols: Mice were sacrificed when ill, i.e. displaying signs of hematopoietic malignancies including weight loss, lethargic behaviour, anaemia and dyspnea. Malignancies were dissected out and homogenised using a 100 um tissue strainer. Homogenised cell suspensions were centrifuged and taken up in 10% DMSO and 10% FBS in RPMI and stored at -80 degrees Celsius until RNA isolation. RNA was isolated using the RNeasy mini kit (Qiagen) and RNA quality was examined using the Agilent 2100 Bioanalyzer (Agilent Technologies). Typically 300 ng of total RNA was used as input for enrichment by NEXTflex Poly(A) Beads (Bioo Scientific) followed by library preparation using NEXTflex Rapid Directional qRNA-Seq Kit (Bioo Scientific).

text: adult

characteristic: developmental stage

text: Mad2 PiggyBac Mx1-Cre

characteristic: genotype

characteristic: individual

text: not applicable

characteristic: isolate

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spleen

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex

text: mixed C57BL/6 genetic background

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: European Research Institute for the Biology of Ageing University Medical Center Groningen

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

characteristic: broker name

text: tumor single-cell suspension

text: house mouse

text: adult

text: Mad2 PiggyBac Mx1-Cre

text: not applicable

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: male

text: mixed C57BL/6 genetic background


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ArrayExpress

text: tumor single-cell suspension

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: Mad2 PiggyBac Mx1-Cre

text: not applicable

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: female

text: mixed C57BL/6 genetic background

characteristic: title


text: public

characteristic: age

text: ArrayExpress

text: tumor single-cell suspension

text: house mouse

text: adult

characteristic: developmental stage

text: Mad2 PiggyBac Mx1-Cre

characteristic: genotype

characteristic: individual

text: not applicable

characteristic: isolate

text: Mus musculus

characteristic: organism

text: Mus musculus

text: thymus

characteristic: organism part

text: Mus musculus

text: female

text: mixed C57BL/6 genetic background


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: tumor single-cell suspension

text: house mouse

text: adult

text: Mad2 PiggyBac Mx1-Cre

text: not applicable

text: Mus musculus

text: Mus musculus

text: thymus

text: Mus musculus

text: male

characteristic: sex

text: mixed C57BL/6 genetic background

characteristic: strain


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC first public

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: tumor single-cell suspension

text: house mouse

characteristic: common name

text: adult

text: PiggyBac Mx1-Cre

text: not applicable

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: female

text: mixed C57BL/6 genetic background


text: public

characteristic: age

text: ArrayExpress

text: tumor single-cell suspension

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: PiggyBac Mx1-Cre

characteristic: genotype

characteristic: individual

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: thymus

text: Mus musculus

text: male

text: mixed C57BL/6 genetic background

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: tumor single-cell suspension

text: house mouse

text: adult

text: PiggyBac Mx1-Cre

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: thymus

characteristic: organism part

text: Mus musculus

text: female

text: mixed C57BL/6 genetic background


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC first public

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: tumor single-cell suspension

text: house mouse

text: adult

text: PiggyBac Mx1-Cre

text: not applicable

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: male

characteristic: sex

text: mixed C57BL/6 genetic background

characteristic: strain


text: Plasmodium falciparum

characteristic: ArrayExpress-SPECIES

text: SC

text: public

text: malaria parasite P. falciparum

characteristic: common name

text: Plasmodium falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum


text: UNIVERSITY OF AMSTERDAM

text: public

tag: Namespace:UNIVERSITY OF AMSTERDAM

unit: g

characteristic: amount or size of sample collected

text: Pietro Zocca

characteristic: collection date

text: tomato

text: knock-down (RNAi 35S:ir-SlMYC1)

characteristic: genotype

text: Netherlands

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: North Holland, Amsterdam

characteristic: geographic location (region and locality)

text: glasshouse

characteristic: growth facility

text: Qiagen RNeasy Plant Mini Kit (50) (Cat NoID : 74904) for RNA isolation and purification and Qiagen RNase-Free DNase Set (50) (Cat NoID : 79254) for on-columd DNAse treatment

characteristic: isolation and growth condition

unit: h

characteristic: light duration per day (24h)

text: Solanum lycopersicum

text: tomato

characteristic: organism common name

characteristic: plant developmental stage

text: soil

characteristic: plant growth medium

characteristic: plant structure

text: diploid

characteristic: ploidy

characteristic: radiation regimen

text: manual collection of two leaflets of the fourth fully developed leaf, in 50ml falcon tubes

characteristic: sample collection device or method

text: healthy

characteristic: sample disease status

text: healthy

characteristic: sample health state

text: frozen in liquid N2 and homogenized into frozen-powder by vortexing

characteristic: sample material processing

text: 4 weeks

characteristic: sampled age

text: Solanum lycopersicum

text: MYC1-related sample

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF AMSTERDAM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: Pietro Zocca

characteristic: collection date

text: tomato

characteristic: common name

text: knock-out (CRISPR-Cas9)

characteristic: genotype

text: Netherlands

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: North Holland, Amsterdam

text: glasshouse

text: Solanum lycopersicum

characteristic: organism

text: tomato

text: soil

text: diploid

text: healthy

text: healthy

text: 4 weeks

text: Solanum lycopersicum

text: MYC1-related sample


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF AMSTERDAM

text: public

text: Pietro Zocca

text: tomato

text: over-expressor (35S::MYC1e161k)

text: Netherlands

text: North Holland, Amsterdam

characteristic: geographic location (region and locality)

text: glasshouse

characteristic: growth facility

characteristic: isolation and growth condition

characteristic: light duration per day (24h)

text: Solanum lycopersicum

text: tomato

characteristic: organism common name

characteristic: plant developmental stage

text: soil

characteristic: plant growth medium

characteristic: plant structure

text: diploid

characteristic: ploidy

characteristic: radiation regimen

characteristic: sample collection device or method

text: healthy

characteristic: sample disease status

text: healthy

characteristic: sample health state

characteristic: sample material processing

text: 4 weeks

characteristic: sampled age

text: Solanum lycopersicum

text: MYC1-related sample

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF AMSTERDAM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: Pietro Zocca

characteristic: collection date

text: tomato

characteristic: common name

text: wild-type

characteristic: genotype

text: Netherlands

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: North Holland, Amsterdam

text: glasshouse

text: Solanum lycopersicum

characteristic: organism

text: tomato

text: soil

text: diploid

text: healthy

text: healthy

text: 4 weeks

text: Solanum lycopersicum

text: MYC1-related sample


E-MTAB-11368:Sample 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: DEPARTMENT OF GENETICS

text: public

text: E-MTAB-11368:Sample 2

tag: Namespace:DEPARTMENT OF GENETICS

text: 17 to 18

unit: day

characteristic: age

text: ArrayExpress

characteristic: broker name

text: neuron

text: Norway rat

text: Protocols: Primary hippocampal cells from rat embryos were grown in gels of different stiffnesses (10kpa and 100pa) attached to ibidi 35mm diameter glass bottom dishes. 2 separate CRISPR assays in parallel (H1 and H2). Each of those assays has been carried out by electroporating 4 different CRISPR guides into the cells per assay. All 8 guides are specific for the PIEZO1 gene. TOPO cloning with subsequent Sanger sequencing has shown random variations for the different guides in the guide regions (or close to it). But the efficiency of the KD is low. We estimate between 25% and 40%. For RNA isolation culture medium was taken off and 350ul RLT plus buffer containing beta mercapto ethanol was added to the gel containing the cells. The gel containing the cells was scraped off the dish (Cell Scraper Greiner Bio one), transferred to a 1.5 ml Eppendorf and then homogenized by pipetting up and down (x50) using a blue tip cut off to increase the tip opening. Each lysate was then vortexed 30 sec. RNA purification was performed as described by RNeasy plus Micro kit (QIAGEN #74034). Samples were eluted in 20ul of RNase-free H2O and quantified measuring UV absorption at 260 nm with a spectrophotometer plate reader, the SpectroStar (BMG Labtech). Libraries were generated using the the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech Laboratories)

characteristic: description

text: embryo stage

characteristic: developmental stage

text: Piezo1 mutant H1

text: 10 kPa hydrogel

characteristic: growth condition

text: pooled offspring from mother 1

characteristic: individual

text: not applicable

characteristic: isolate

text: Rattus norvegicus

text: Rattus norvegicus

text: hippocampus

characteristic: organism part

text: Rattus norvegicus

text: Sprague dawley

characteristic: strain

text: Sample 2

characteristic: title


E-MTAB-11368:Sample 1

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: DEPARTMENT OF GENETICS

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11368:Sample 1

characteristic: Submitter Id

text: 17 to 18

text: ArrayExpress

text: neuron

text: Norway rat

characteristic: common name

text: embryo stage

text: Piezo1 mutant H1

characteristic: genotype

text: 100 Pa hydrogel

text: pooled offspring from mother 1

text: not applicable

text: Rattus norvegicus

text: Rattus norvegicus

text: hippocampus

text: Rattus norvegicus

text: Sprague dawley

text: Sample 1


E-MTAB-11368:Sample 4

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: DEPARTMENT OF GENETICS

text: public

text: E-MTAB-11368:Sample 4

text: 17 to 18

characteristic: age

text: ArrayExpress

characteristic: broker name

text: neuron

text: Norway rat

characteristic: description

text: embryo stage

characteristic: developmental stage

text: Piezo1 mutant H1

text: 10 kPa hydrogel

text: pooled offspring from mother 2

text: not applicable

text: Rattus norvegicus

characteristic: organism

text: Rattus norvegicus

text: hippocampus

text: Rattus norvegicus

text: Sprague dawley

text: Sample 4


E-MTAB-11368:Sample 3

characteristic: ENA-LAST-UPDATE

text: DEPARTMENT OF GENETICS

text: public

text: E-MTAB-11368:Sample 3

text: 17 to 18

text: ArrayExpress

text: neuron

text: Norway rat

text: embryo stage

text: Piezo1 mutant H1

text: 100 Pa hydrogel

characteristic: growth condition

text: pooled offspring from mother 2

characteristic: individual

text: not applicable

characteristic: isolate

text: Rattus norvegicus

text: Rattus norvegicus

text: hippocampus

characteristic: organism part

text: Rattus norvegicus

text: Sprague dawley

characteristic: strain

text: Sample 3

characteristic: title


E-MTAB-11368:Sample 6

characteristic: External Id

text: DEPARTMENT OF GENETICS

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11368:Sample 6

characteristic: Submitter Id

text: 17 to 18

text: ArrayExpress

text: neuron

text: Norway rat

text: embryo stage

text: Piezo1 mutant H1

text: 10 kPa hydrogel

text: pooled offspring from mother 3

text: not applicable

text: Rattus norvegicus

text: Rattus norvegicus

text: hippocampus

text: Rattus norvegicus

text: Sprague dawley

text: Sample 6


E-MTAB-11368:Sample 5

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: DEPARTMENT OF GENETICS

text: public

text: E-MTAB-11368:Sample 5

text: 17 to 18

characteristic: age

text: ArrayExpress

characteristic: broker name

text: neuron

text: Norway rat

characteristic: common name

characteristic: description

text: embryo stage

text: Piezo1 mutant H1

characteristic: genotype

text: 100 Pa hydrogel

text: pooled offspring from mother 3

text: not applicable

text: Rattus norvegicus

text: Rattus norvegicus

text: hippocampus

text: Rattus norvegicus

text: Sprague dawley

text: Sample 5


E-MTAB-11368:Sample 8

characteristic: ENA-LAST-UPDATE

text: DEPARTMENT OF GENETICS

text: public

text: E-MTAB-11368:Sample 8

text: 17 to 18

text: ArrayExpress

text: neuron

text: Norway rat

text: embryo stage

characteristic: developmental stage

text: Piezo1 mutant H1

text: 10 kPa hydrogel

characteristic: growth condition

text: pooled offspring from mother 4

characteristic: individual

text: not applicable

characteristic: isolate

text: Rattus norvegicus

characteristic: organism

text: Rattus norvegicus

text: hippocampus

characteristic: organism part

text: Rattus norvegicus

text: Sprague dawley

text: Sample 8


E-MTAB-11368:Sample 7

characteristic: External Id

text: DEPARTMENT OF GENETICS

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11368:Sample 7

text: 17 to 18

text: ArrayExpress

text: neuron

text: Norway rat

text: embryo stage

text: Piezo1 mutant H1

text: 100 Pa hydrogel

text: pooled offspring from mother 4

text: not applicable

text: Rattus norvegicus

text: Rattus norvegicus

text: hippocampus

text: Rattus norvegicus

text: Sprague dawley

characteristic: strain

text: Sample 7

characteristic: title


E-MTAB-11368:Sample 10

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: DEPARTMENT OF GENETICS

text: public

characteristic: SRA accession

text: E-MTAB-11368:Sample 10

characteristic: Submitter Id

text: 17 to 18

characteristic: age

text: ArrayExpress

characteristic: broker name

text: neuron

text: Norway rat

characteristic: common name

text: embryo stage

text: Piezo1 mutant H2

text: 10 kPa hydrogel

text: pooled offspring from mother 1

text: not applicable

text: Rattus norvegicus

text: Rattus norvegicus

text: hippocampus

text: Rattus norvegicus

text: Sprague dawley

text: Sample 10


E-MTAB-11368:Sample 9

characteristic: ENA-LAST-UPDATE

text: DEPARTMENT OF GENETICS

text: public

text: E-MTAB-11368:Sample 9

text: 17 to 18

text: ArrayExpress

text: neuron

text: Norway rat

characteristic: description

text: embryo stage

characteristic: developmental stage

text: Piezo1 mutant H2

characteristic: genotype

text: 100 Pa hydrogel

characteristic: growth condition

text: pooled offspring from mother 1

characteristic: individual

text: not applicable

characteristic: isolate

text: Rattus norvegicus

text: Rattus norvegicus

text: hippocampus

text: Rattus norvegicus

text: Sprague dawley

text: Sample 9


E-MTAB-11368:Sample 12

characteristic: External Id

text: DEPARTMENT OF GENETICS

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11368:Sample 12

text: 17 to 18

text: ArrayExpress

text: neuron

text: Norway rat

text: embryo stage

text: Piezo1 mutant H2

text: 10 kPa hydrogel

text: pooled offspring from mother 2

text: not applicable

text: Rattus norvegicus

characteristic: organism

text: Rattus norvegicus

text: hippocampus

characteristic: organism part

text: Rattus norvegicus

text: Sprague dawley

text: Sample 12


E-MTAB-11368:Sample 11

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: DEPARTMENT OF GENETICS

text: public

characteristic: SRA accession

text: E-MTAB-11368:Sample 11

characteristic: Submitter Id

text: 17 to 18

characteristic: age

text: ArrayExpress

text: neuron

text: Norway rat

text: embryo stage

text: Piezo1 mutant H2

text: 100 Pa hydrogel

text: pooled offspring from mother 2

text: not applicable

text: Rattus norvegicus

text: Rattus norvegicus

text: hippocampus

text: Rattus norvegicus

text: Sprague dawley

characteristic: strain

text: Sample 11

characteristic: title


E-MTAB-11368:Sample 14

characteristic: ENA-LAST-UPDATE

text: DEPARTMENT OF GENETICS

text: public

text: E-MTAB-11368:Sample 14

text: 17 to 18

text: ArrayExpress

characteristic: broker name

text: neuron

text: Norway rat

characteristic: common name

characteristic: description

text: embryo stage

characteristic: developmental stage

text: Piezo1 mutant H2

characteristic: genotype

text: 10 kPa hydrogel

characteristic: growth condition

text: pooled offspring from mother 3

text: not applicable

text: Rattus norvegicus

text: Rattus norvegicus

text: hippocampus

text: Rattus norvegicus

text: Sprague dawley

text: Sample 14


E-MTAB-11368:Sample 13

characteristic: External Id

text: DEPARTMENT OF GENETICS

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11368:Sample 13

text: 17 to 18

text: ArrayExpress

text: neuron

text: Norway rat

text: embryo stage

text: Piezo1 mutant H2

text: 100 Pa hydrogel

text: pooled offspring from mother 3

characteristic: individual

text: not applicable

characteristic: isolate

text: Rattus norvegicus

text: Rattus norvegicus

text: hippocampus

text: Rattus norvegicus

text: Sprague dawley

text: Sample 13


E-MTAB-11368:Sample 16

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: DEPARTMENT OF GENETICS

text: public

characteristic: SRA accession

text: E-MTAB-11368:Sample 16

characteristic: Submitter Id

text: 17 to 18

characteristic: age

text: ArrayExpress

text: neuron

text: Norway rat

text: embryo stage

text: Piezo1 mutant H2

text: 10 kPa hydrogel

text: pooled offspring from mother 4

text: not applicable

text: Rattus norvegicus

characteristic: organism

text: Rattus norvegicus

text: hippocampus

characteristic: organism part

text: Rattus norvegicus

text: Sprague dawley

text: Sample 16


E-MTAB-11368:Sample 15

characteristic: ENA-LAST-UPDATE

text: DEPARTMENT OF GENETICS

text: public

text: E-MTAB-11368:Sample 15

text: 17 to 18

text: ArrayExpress

characteristic: broker name

text: neuron

text: Norway rat

characteristic: common name

characteristic: description

text: embryo stage

characteristic: developmental stage

text: Piezo1 mutant H2

text: 100 Pa hydrogel

text: pooled offspring from mother 4

text: not applicable

text: Rattus norvegicus

text: Rattus norvegicus

text: hippocampus

text: Rattus norvegicus

text: Sprague dawley

characteristic: strain

text: Sample 15

characteristic: title


E-MTAB-11368:Sample 18

characteristic: External Id

text: DEPARTMENT OF GENETICS

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11368:Sample 18

text: 17 to 18

text: ArrayExpress

text: neuron

text: Norway rat

text: embryo stage

text: wild type genotype

characteristic: genotype

text: 10 kPa hydrogel

characteristic: growth condition

text: pooled offspring from mother 1

text: not applicable

text: Rattus norvegicus

text: Rattus norvegicus

text: hippocampus

text: Rattus norvegicus

text: Sprague dawley

text: Sample 18


E-MTAB-11368:Sample 17

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: DEPARTMENT OF GENETICS

text: public

characteristic: SRA accession

text: E-MTAB-11368:Sample 17

characteristic: Submitter Id

text: 17 to 18

characteristic: age

text: ArrayExpress

text: neuron

text: Norway rat

text: embryo stage

text: wild type genotype

text: 100 Pa hydrogel

text: pooled offspring from mother 1

characteristic: individual

text: not applicable

characteristic: isolate

text: Rattus norvegicus

text: Rattus norvegicus

text: hippocampus

text: Rattus norvegicus

text: Sprague dawley

text: Sample 17


E-MTAB-11368:Sample 20

characteristic: ENA-LAST-UPDATE

text: DEPARTMENT OF GENETICS

text: public

text: E-MTAB-11368:Sample 20

text: 17 to 18

text: ArrayExpress

characteristic: broker name

text: neuron

text: Norway rat

characteristic: common name

characteristic: description

text: embryo stage

characteristic: developmental stage

text: wild type genotype

text: 10 kPa hydrogel

text: pooled offspring from mother 2

text: not applicable

text: Rattus norvegicus

characteristic: organism

text: Rattus norvegicus

text: hippocampus

characteristic: organism part

text: Rattus norvegicus

text: Sprague dawley

text: Sample 20


E-MTAB-11368:Sample 19

characteristic: External Id

text: DEPARTMENT OF GENETICS

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11368:Sample 19

text: 17 to 18

text: ArrayExpress

text: neuron

text: Norway rat

text: embryo stage

text: wild type genotype

text: 100 Pa hydrogel

text: pooled offspring from mother 2

text: not applicable

text: Rattus norvegicus

text: Rattus norvegicus

text: hippocampus

text: Rattus norvegicus

text: Sprague dawley

characteristic: strain

text: Sample 19

characteristic: title


E-MTAB-11368:Sample 22

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: DEPARTMENT OF GENETICS

text: public

characteristic: SRA accession

text: E-MTAB-11368:Sample 22

characteristic: Submitter Id

text: 17 to 18

characteristic: age

text: ArrayExpress

text: neuron

text: Norway rat

text: embryo stage

text: wild type genotype

characteristic: genotype

text: 10 kPa hydrogel

characteristic: growth condition

text: pooled offspring from mother 3

text: not applicable

text: Rattus norvegicus

text: Rattus norvegicus

text: hippocampus

text: Rattus norvegicus

text: Sprague dawley

text: Sample 22


E-MTAB-11368:Sample 21

characteristic: ENA-LAST-UPDATE

text: DEPARTMENT OF GENETICS

text: public

text: E-MTAB-11368:Sample 21

text: 17 to 18

text: ArrayExpress

characteristic: broker name

text: neuron

text: Norway rat

characteristic: common name

characteristic: description

text: embryo stage

characteristic: developmental stage

text: wild type genotype

text: 100 Pa hydrogel

text: pooled offspring from mother 3

characteristic: individual

text: not applicable

characteristic: isolate

text: Rattus norvegicus

text: Rattus norvegicus

text: hippocampus

text: Rattus norvegicus

text: Sprague dawley

text: Sample 21


E-MTAB-11368:Sample 24

characteristic: External Id

text: DEPARTMENT OF GENETICS

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11368:Sample 24

text: 17 to 18

text: ArrayExpress

text: neuron

text: Norway rat

text: embryo stage

text: wild type genotype

text: 10 kPa hydrogel

text: pooled offspring from mother 4

text: not applicable

text: Rattus norvegicus

characteristic: organism

text: Rattus norvegicus

text: hippocampus

characteristic: organism part

text: Rattus norvegicus

text: Sprague dawley

text: Sample 24


E-MTAB-11368:Sample 23

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: DEPARTMENT OF GENETICS

text: public

characteristic: SRA accession

text: E-MTAB-11368:Sample 23

characteristic: Submitter Id

text: 17 to 18

characteristic: age

text: ArrayExpress

text: neuron

text: Norway rat

text: embryo stage

text: wild type genotype

text: 100 Pa hydrogel

text: pooled offspring from mother 4

text: not applicable

text: Rattus norvegicus

text: Rattus norvegicus

text: hippocampus

text: Rattus norvegicus

text: Sprague dawley

characteristic: strain

text: Sample 23

characteristic: title


characteristic: ENA-LAST-UPDATE

text: Finnish Food Authority

text: public

tag: Namespace:Finnish Food Authority

text: Finnish Food Authority

characteristic: collected by

characteristic: collection date

text: Finland

characteristic: geographic location (country and/or sea)

text: Canis lupus familiaris

characteristic: host scientific name

text: the intestinal tract of dog

characteristic: isolation source

text: Escherichia coli

text: Escherichia coli

text: Escherichia coli from dog


characteristic: External Id

text: Finnish Food Authority

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Finnish Food Authority

text: Finland

text: Canis lupus familiaris

characteristic: isolate

text: the intestinal tract of dog

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae from dog


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Finnish Food Authority

text: public

characteristic: INSDC status

text: Finnish Food Authority

text: Finland

text: not applicable

characteristic: host health state

text: Canis lupus familiaris

text: the intestinal tract of dog

text: Escherichia coli

characteristic: organism

text: Escherichia coli

text: Escherichia coli from dog


text: Finnish Food Authority

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Finnish Food Authority

text: Finland

text: Canis lupus familiaris

text: the intestinal tract of dog

text: Escherichia coli

text: Escherichia coli

text: Escherichia coli/albertii from dog

characteristic: title


E-MTAB-11457:Sample 10

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-11457:Sample 10

tag: Namespace:UNIVERSITY OF CAMBRIDGE

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: Protocols: Fetuses were killed by decapitation and livers rapidly collected, weighed and snap frozen in liquid nitrogen. Samples were stored at -80C RNA isolated using a RNeasy Plus Mini Kit (Qiagen, UK). Illumina TruSeq Stranded mRNA library preparation kit (Illumina))

characteristic: description

text: late embryonic stage

characteristic: developmental stage

text: control

characteristic: genotype

characteristic: individual

text: not applicable

text: Mus musculus

text: Mus musculus

text: liver

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex

characteristic: strain

text: Sample 10


E-MTAB-11457:Sample 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11457:Sample 2

text: ArrayExpress

text: house mouse

text: late embryonic stage

text: control

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: liver

text: Mus musculus

text: female

text: Sample 2


E-MTAB-11457:Sample 4

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF CAMBRIDGE

text: public

characteristic: SRA accession

text: E-MTAB-11457:Sample 4

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

text: late embryonic stage

text: control

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: liver

text: Mus musculus

text: female

text: Sample 4

characteristic: title


E-MTAB-11457:Sample 5

text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-11457:Sample 5

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: late embryonic stage

characteristic: developmental stage

text: control

characteristic: genotype

characteristic: individual

text: not applicable

text: Mus musculus

text: Mus musculus

text: liver

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: Sample 5


E-MTAB-11457:Sample 6

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11457:Sample 6

text: ArrayExpress

text: house mouse

text: late embryonic stage

text: control

text: not applicable

text: Mus musculus

text: Mus musculus

text: liver

text: Mus musculus

text: male

characteristic: strain

text: Sample 6


E-MTAB-11457:Sample 9

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF CAMBRIDGE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11457:Sample 9

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

text: late embryonic stage

text: control

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: liver

text: Mus musculus

text: male

text: Sample 9


E-MTAB-11457:Sample 1

text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-11457:Sample 1

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: late embryonic stage

characteristic: developmental stage

text: Igf2 and H19 mutant expression in placenta

characteristic: genotype

characteristic: individual

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: liver

text: Mus musculus

text: male

text: Sample 1

characteristic: title


E-MTAB-11457:Sample 11

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11457:Sample 11

text: ArrayExpress

text: house mouse

text: late embryonic stage

text: not applicable

text: Mus musculus

text: Mus musculus

text: liver

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: Sample 11


E-MTAB-11457:Sample 12

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11457:Sample 12

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: house mouse

text: late embryonic stage

text: not applicable

text: Mus musculus

text: Mus musculus

text: liver

text: Mus musculus

text: female

characteristic: strain

text: Sample 12


E-MTAB-11457:Sample 13

text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-11457:Sample 13

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

characteristic: description

text: late embryonic stage

characteristic: developmental stage

characteristic: genotype

characteristic: individual

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: liver

text: Mus musculus

text: female

text: Sample 13


E-MTAB-11457:Sample 14

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11457:Sample 14

text: ArrayExpress

text: house mouse

text: late embryonic stage

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: liver

text: Mus musculus

text: female

text: Sample 14

characteristic: title


E-MTAB-11457:Sample 3

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11457:Sample 3

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: late embryonic stage

text: not applicable

text: Mus musculus

text: Mus musculus

text: liver

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex

text: Sample 3


E-MTAB-11457:Sample 7

text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-11457:Sample 7

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

characteristic: description

text: late embryonic stage

characteristic: developmental stage

characteristic: genotype

text: not applicable

text: Mus musculus

text: Mus musculus

text: liver

text: Mus musculus

text: male

characteristic: strain

text: Sample 7


E-MTAB-11457:Sample 8

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11457:Sample 8

text: ArrayExpress

text: house mouse

text: late embryonic stage

characteristic: individual

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: liver

text: Mus musculus

text: male

text: Sample 8


characteristic: ENA-LAST-UPDATE

text: NEWCASTLE UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:NEWCASTLE UNIVERSITY

characteristic: Submitter Id

text: ArrayExpress

text: human

text: Protocols: Samples were collected from the clinic and dissected before storage in hypothermosol over night. Tissues were digested using collagenase type 4 and trypsin before dead cell removal using FACS. Nucleic acids were extracted as part of the 10x Genomics Chromium Next GEM Single Cell 5' Reagent Kits v2 (Dual Index) protocol. Nucleic acid libraries were constructed as part of the 10x Genomics Chromium Next GEM Single Cell 5' Reagent Kits v2 (Dual Index) protocol.

text: embryonic stage

text: normal

characteristic: disease

text: Carnegie stage 16

characteristic: embryonic stage

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: embryo

text: section one

characteristic: sampling site

text: Homo sapiens

text: male

characteristic: title


characteristic: ENA-CHECKLIST

text: NEWCASTLE UNIVERSITY

text: public

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

characteristic: description

text: embryonic stage

characteristic: developmental stage

text: normal

text: Carnegie stage 16

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: embryo

characteristic: organism part

text: section two

text: Homo sapiens

text: male

characteristic: sex