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characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: human-gut

text: metagenome

text: human gut metagenome

characteristic: organism

characteristic: project name

text: human gut metagenome

text: Illumina

text: DNA metagenome from sample CGM21120

characteristic: title


text: FISABIO

characteristic: INSDC center name

text: public

text: human gut metagenome

characteristic: common name

text: DNA metagenome from subject CGM21121

text: terrestrial biome

characteristic: environment (biome)

text: human-associated habitat

characteristic: environment (feature)

text: human-associated habitat

characteristic: environment (material)

text: Spain

text: human-gut

characteristic: human gut environmental package

text: metagenome

characteristic: investigation type

text: human gut metagenome

text: human gut metagenome

text: Illumina

characteristic: sequencing method

text: DNA metagenome from sample CGM21121


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: FISABIO

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: human gut metagenome

text: DNA metagenome from subject CGM21122

text: terrestrial biome

text: human-associated habitat

text: human-associated habitat

text: Spain

text: human-gut

text: metagenome

text: human gut metagenome

text: human gut metagenome

text: Illumina

text: DNA metagenome from sample CGM21122


text: Skin

characteristic: ArrayExpress-PHENOTYPE

text: female

characteristic: ArrayExpress-SEX

text: Felis catus

characteristic: ArrayExpress-SPECIES

text: Female

characteristic: GENDER

text: SC

text: public

text: Skin

characteristic: PHENOTYPE

characteristic: Submitter Id

text: domestic cat

text: Felis catus

characteristic: organism

text: Felis catus

characteristic: title


text: Skin

text: male

text: Felis catus

text: Male

text: SC

characteristic: INSDC center name

text: public

text: Skin

text: domestic cat

characteristic: common name

text: Felis catus

text: Felis catus


text: Bone

text: male

text: Felis catus

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Male

text: SC

text: public

text: Bone

text: domestic cat

text: Felis catus

text: Felis catus


text: Stomach

text: male

text: Felis catus

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Male

text: SC

text: public

text: Stomach

text: domestic cat

text: Felis catus

text: Felis catus


text: Liver

text: male

text: Felis catus

text: Male

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Liver

text: domestic cat

text: Felis catus

text: Felis catus


text: Heart

text: male

text: Felis catus

text: Male

text: SC

text: public

characteristic: INSDC status

text: Heart

characteristic: SRA accession

text: domestic cat

text: Felis catus

text: Felis catus


text: Bone

characteristic: ArrayExpress-PHENOTYPE

text: female

characteristic: ArrayExpress-SEX

text: Felis catus

text: Female

text: SC

text: public

text: Bone

text: domestic cat

text: Felis catus

text: Felis catus


text: Skeletal muscle

text: female

text: Felis catus

characteristic: ArrayExpress-SPECIES

text: Female

characteristic: GENDER

text: SC

text: public

text: Skeletal muscle

text: domestic cat

text: Felis catus

text: Felis catus


text: Kidney

text: male

text: Felis catus

text: Male

text: SC

text: public

text: Kidney

characteristic: PHENOTYPE

characteristic: Submitter Id

text: domestic cat

text: Felis catus

text: Felis catus


text: Femur

text: female

text: Felis catus

text: Female

text: SC

text: public

text: Femur

text: domestic cat

text: Felis catus

characteristic: organism

text: Felis catus

characteristic: title


text: Scapula

text: male

text: Felis catus

text: Male

text: SC

characteristic: INSDC center name

text: public

text: Scapula

text: domestic cat

characteristic: common name

text: Felis catus

text: Felis catus


text: Skull

text: male

text: Felis catus

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Male

text: SC

text: public

text: Skull

text: domestic cat

text: Felis catus

text: Felis catus


text: Femur

text: male

text: Felis catus

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Male

text: SC

text: public

text: Femur

text: domestic cat

text: Felis catus

text: Felis catus


text: Skull

text: female

text: Felis catus

text: Female

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Skull

text: domestic cat

text: Felis catus

text: Felis catus


text: Muscle

text: male

text: Felis catus

text: Male

text: SC

text: public

characteristic: INSDC status

text: Muscle

characteristic: SRA accession

text: domestic cat

text: Felis catus

text: Felis catus


text: Bone

characteristic: ArrayExpress-PHENOTYPE

text: unknown

characteristic: ArrayExpress-SEX

text: Felis catus

text: Unknown

text: SC

text: public

text: Bone

text: domestic cat

text: Felis catus

text: Felis catus


text: Muscle

text: unknown

text: Felis catus

characteristic: ArrayExpress-SPECIES

text: Unknown

characteristic: GENDER

text: SC

text: public

text: Muscle

text: domestic cat

text: Felis catus

text: Felis catus


text: Nasal mucosa

text: male

text: Felis catus

text: Male

text: SC

text: public

text: Nasal mucosa

characteristic: PHENOTYPE

characteristic: Submitter Id

text: domestic cat

text: Felis catus

text: Felis catus


text: Skin

text: unknown

text: Felis catus

text: Unknown

text: SC

text: public

text: Skin

text: domestic cat

text: Felis catus

characteristic: organism

text: Felis catus

characteristic: title


text: Skin

text: female

text: Felis catus

text: Female

text: SC

characteristic: INSDC center name

text: public

text: Skin

text: domestic cat

characteristic: common name

text: Felis catus

text: Felis catus


text: Muscle

text: female

text: Felis catus

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Female

text: SC

text: public

text: Muscle

text: domestic cat

text: Felis catus

text: Felis catus


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: WDRC KAUST

text: public

tag: Namespace:WDRC KAUST

text: wastewater biofilm

characteristic: broad-scale environmental context

characteristic: collection date

text: Reactor A T1a run1

characteristic: description

text: wastewater biofilm

characteristic: environmental medium

text: Saudi Arabia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: wastewater biofilm

characteristic: local environmental context

text: wastewater metagenome

text: Understanding the role of sorption and biodegradation in the removal of organic micropollutants by MABR with different biofilm thickness

characteristic: project name

text: wastewater metagenome

text: Reactor A T1a run1


text: WDRC KAUST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: wastewater biofilm

text: Reactor A T1b run1

text: wastewater biofilm

text: Saudi Arabia

text: wastewater biofilm

text: wastewater metagenome

characteristic: organism

text: wastewater metagenome

text: Reactor A T1b run1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: WDRC KAUST

characteristic: INSDC center name

text: public

text: wastewater biofilm

text: Reactor B T1a run1

text: wastewater biofilm

text: Saudi Arabia

text: wastewater biofilm

text: wastewater metagenome

text: wastewater metagenome

text: Reactor B T1a run1

characteristic: title


characteristic: External Id

text: WDRC KAUST

text: public

text: wastewater biofilm

characteristic: broad-scale environmental context

characteristic: collection date

text: Reactor A T2a run1

characteristic: description

text: wastewater biofilm

characteristic: environmental medium

text: Saudi Arabia

text: wastewater biofilm

text: wastewater metagenome

text: wastewater metagenome

text: Reactor A T2a run1


text: WDRC KAUST

text: public

text: wastewater biofilm

text: Reactor A T2b run1

text: wastewater biofilm

text: Saudi Arabia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: wastewater biofilm

characteristic: local environmental context

text: wastewater metagenome

characteristic: project name

text: wastewater metagenome

text: Reactor A T2b run1


text: WDRC KAUST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: wastewater biofilm

text: Reactor B T2a run1

text: wastewater biofilm

text: Saudi Arabia

text: wastewater biofilm

text: wastewater metagenome

text: wastewater metagenome

text: Reactor B T2a run1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: WDRC KAUST

characteristic: INSDC center name

text: public

text: wastewater biofilm

text: Reactor B T2b run1

text: wastewater biofilm

text: Saudi Arabia

text: wastewater biofilm

text: wastewater metagenome

characteristic: organism

text: wastewater metagenome

text: Reactor B T2b run1


characteristic: External Id

text: WDRC KAUST

text: public

text: wastewater biofilm

characteristic: broad-scale environmental context

characteristic: collection date

text: Reactor A T3a run1

characteristic: description

text: wastewater biofilm

text: Saudi Arabia

text: wastewater biofilm

text: wastewater metagenome

text: wastewater metagenome

text: Reactor A T3a run1

characteristic: title


text: WDRC KAUST

text: public

text: wastewater biofilm

text: Reactor A T3b run1

text: wastewater biofilm

characteristic: environmental medium

text: Saudi Arabia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: wastewater biofilm

characteristic: local environmental context

text: wastewater metagenome

text: wastewater metagenome

text: Reactor A T3b run1


text: WDRC KAUST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: wastewater biofilm

text: Reactor B T3a run1

text: wastewater biofilm

text: Saudi Arabia

text: wastewater biofilm

text: wastewater metagenome

characteristic: project name

text: wastewater metagenome

text: Reactor B T3a run1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: WDRC KAUST

characteristic: INSDC center name

text: public

characteristic: Submitter Id

text: wastewater biofilm

text: Reactor B T3b run1

text: wastewater biofilm

text: Saudi Arabia

text: wastewater biofilm

text: wastewater metagenome

text: wastewater metagenome

text: Reactor B T3b run1


characteristic: External Id

text: WDRC KAUST

text: public

text: wastewater biofilm

characteristic: broad-scale environmental context

characteristic: collection date

text: Reactor A T1a run2

characteristic: description

text: wastewater biofilm

text: Saudi Arabia

text: wastewater biofilm

text: wastewater metagenome

characteristic: organism

text: wastewater metagenome

text: Reactor A T1a run2


text: WDRC KAUST

text: public

text: wastewater biofilm

text: Reactor A T1b run2

text: wastewater biofilm

characteristic: environmental medium

text: Saudi Arabia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: wastewater biofilm

text: wastewater metagenome

text: wastewater metagenome

text: Reactor A T1b run2

characteristic: title


text: WDRC KAUST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: wastewater biofilm

text: Reactor B T1a run2

text: wastewater biofilm

text: Saudi Arabia

text: wastewater biofilm

characteristic: local environmental context

text: wastewater metagenome

text: wastewater metagenome

text: Reactor B T1a run2


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: WDRC KAUST

characteristic: INSDC center name

text: public

text: wastewater biofilm

text: Reactor B T1b run2

text: wastewater biofilm

text: Saudi Arabia

text: wastewater biofilm

text: wastewater metagenome

characteristic: project name

text: wastewater metagenome

text: Reactor B T1b run2


characteristic: External Id

text: WDRC KAUST

text: public

characteristic: Submitter Id

text: wastewater biofilm

characteristic: broad-scale environmental context

characteristic: collection date

text: Reactor A T2a run2

characteristic: description

text: wastewater biofilm

text: Saudi Arabia

text: wastewater biofilm

text: wastewater metagenome

text: wastewater metagenome

text: Reactor A T2a run2


text: WDRC KAUST

text: public

text: wastewater biofilm

text: Reactor A T2b run2

text: wastewater biofilm

characteristic: environmental medium

text: Saudi Arabia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: wastewater biofilm

text: wastewater metagenome

characteristic: organism

text: wastewater metagenome

text: Reactor A T2b run2


text: WDRC KAUST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: wastewater biofilm

text: Reactor B T2a run2

text: wastewater biofilm

text: Saudi Arabia

text: wastewater biofilm

text: wastewater metagenome

text: wastewater metagenome

text: Reactor B T2a run2

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: WDRC KAUST

characteristic: INSDC center name

text: public

text: wastewater biofilm

text: Reactor B T2b run2

text: wastewater biofilm

text: Saudi Arabia

text: wastewater biofilm

characteristic: local environmental context

text: wastewater metagenome

text: wastewater metagenome

text: Reactor B T2b run2


characteristic: External Id

text: WDRC KAUST

text: public

characteristic: Submitter Id

text: wastewater biofilm

characteristic: broad-scale environmental context

characteristic: collection date

text: Reactor A T3a run2

text: wastewater biofilm

text: Saudi Arabia

text: wastewater biofilm

text: wastewater metagenome

characteristic: project name

text: wastewater metagenome

text: Reactor A T3a run2


text: WDRC KAUST

text: public

text: wastewater biofilm

text: Reactor A T3b run2

characteristic: description

text: wastewater biofilm

characteristic: environmental medium

text: Saudi Arabia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: wastewater biofilm

text: wastewater metagenome

text: wastewater metagenome

text: Reactor A T3b run2


text: WDRC KAUST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: wastewater biofilm

text: Reactor B T3a run2

text: wastewater biofilm

text: Saudi Arabia

text: wastewater biofilm

text: wastewater metagenome

characteristic: organism

text: wastewater metagenome

text: Reactor B T3a run2


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: WDRC KAUST

characteristic: INSDC center name

text: public

text: wastewater biofilm

text: Reactor B T3b run2

text: wastewater biofilm

text: Saudi Arabia

text: wastewater biofilm

text: wastewater metagenome

text: wastewater metagenome

text: Reactor B T3b run2

characteristic: title


text: Cell Line

characteristic: ArrayExpress-PHENOTYPE

text: unknown

characteristic: ArrayExpress-SEX

text: Homo sapiens

characteristic: ArrayExpress-SPECIES

characteristic: External Id

text: Unknown

characteristic: GENDER

text: SC

text: public

text: Cell Line

characteristic: PHENOTYPE

characteristic: Submitter Id

text: human

characteristic: common name

text: Homo sapiens

text: Homo sapiens


text: Mus musculus

characteristic: ArrayExpress-Species

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: house mouse

text: Mus musculus

text: Mus musculus

characteristic: strain


characteristic: ENA-CHECKLIST

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:WASHINGTON UNIVERSITY CENTER FOR GENOME SCIENCES

text: metagenome

characteristic: organism

text: metagenome

text: human fecal sample


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: WASHINGTON UNIVERSITY CENTER FOR GENOME SCIENCES

characteristic: INSDC center name

text: public

text: metagenome

text: metagenome

text: human fecal sample

characteristic: title


characteristic: External Id

text: public

tag: Namespace:University of the West Indies-Cave Hill Campus

text: marine benthic biome (ENVO:01000024)

characteristic: broad-scale environmental context

characteristic: collection date

unit: m

characteristic: depth

text: sea beach (ENVO:00000092)

characteristic: environmental medium

text: Barbados

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: algal material (ENVO:01001189)

characteristic: local environmental context

text: macroalgae metagenome

text: 341F (CCTACGGGNGGCWGCAG) and 805R (GACTACHVGGGTATCTAATCC)

characteristic: pcr primers

text: Prokaryotic diversity in Sargassum waste August 2019

characteristic: project name

text: macroalgae metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Sargassum sample-LB


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: University of the West Indies-Cave Hill Campus

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: marine benthic biome (ENVO:01000024)

text: sea beach (ENVO:00000092)

text: Barbados

text: algal material (ENVO:01001189)

text: macroalgae metagenome

characteristic: organism

text: macroalgae metagenome

text: Illumina MiSeq

text: 16S rRNA

text: Sargassum sample-HS


characteristic: External Id

characteristic: INSDC center name

text: public

tag: Namespace:Key Laboratory of Environmental and Applied Microbiology, CAS

text: Soil

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

characteristic: elevation

text: Soil

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Soil

text: gut metagenome

text: Soil

text: gut metagenome

text: Soil

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Key Laboratory of Environmental and Applied Microbiology, CAS

text: public

text: Soil

text: Soil

text: China

characteristic: geographic location (longitude)

text: Soil

characteristic: local environmental context

text: gut metagenome

text: Soil

characteristic: project name

text: gut metagenome

text: Soil


text: WEIZMANN INSTITUTE OF SCIENCE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:WEIZMANN INSTITUTE OF SCIENCE

characteristic: Submitter Id

text: human

characteristic: common name

text: Homo sapiens

characteristic: organism

text: Homo sapiens


characteristic: External Id

text: IZSLER

characteristic: INSDC center name

text: public

characteristic: collection date

text: Genomic study of Salmonella serovars isolated in wild boars

characteristic: description

text: Italy

text: not provided

characteristic: host health state

text: Gallus gallus

characteristic: host scientific name

characteristic: isolate

text: environmental

text: Salmonella enterica

text: Salmonella enterica


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: IZSLER

text: public

text: Italy

characteristic: geographic location (country and/or sea)

text: not provided

text: homo sapiens

text: feces

text: Salmonella enterica

text: Salmonella enterica

characteristic: title


text: IZSLER

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Italy

text: not provided

text: homo sapiens

text: not provided

text: Salmonella enterica

text: Salmonella enterica


text: IZSLER

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Italy

text: not provided

text: Gallus gallus

text: animal

text: Salmonella enterica

text: Salmonella enterica


characteristic: External Id

text: IZSLER

text: public

text: Italy

text: not provided

text: homo sapiens

text: urine

text: Salmonella enterica

characteristic: organism

text: Salmonella enterica


text: IZSLER

characteristic: INSDC center name

text: public

characteristic: collection date

text: Italy

text: not provided

text: Sus scrofa

text: not provided

text: Salmonella enterica

text: Salmonella enterica


text: IZSLER

text: public

characteristic: description

text: Italy

text: not provided

characteristic: host health state

text: Sus scrofa

text: mesearic lymph nodes

text: Salmonella enterica

text: Salmonella enterica


text: IZSLER

text: public

text: Italy

text: not provided

text: Sus scrofa

characteristic: host scientific name

characteristic: isolate

text: lymph node

text: Salmonella enterica

text: Salmonella enterica


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IZSLER

text: public

text: Italy

text: not provided

text: Bos taurus

text: animal

text: Salmonella enterica

text: Salmonella enterica


characteristic: ENA-LAST-UPDATE

text: IZSLER

text: public

text: Italy

characteristic: geographic location (country and/or sea)

text: not provided

text: Gallus gallus

text: environmental swab

text: Salmonella enterica

text: Salmonella enterica


text: IZSLER

characteristic: INSDC first public

text: public

text: Italy

text: not provided

text: Bos taurus

text: not provided

text: Salmonella enterica

text: Salmonella enterica

characteristic: title


text: IZSLER

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Italy

text: not provided

text: not provided

text: environmental

text: Salmonella enterica

text: Salmonella enterica


text: IZSLER

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Italy

text: not provided

text: Bos taurus

text: rectal swab

text: Salmonella enterica

text: Salmonella enterica


characteristic: External Id

text: IZSLER

text: public

text: Italy

text: not provided

text: Bos taurus

text: feces

text: Salmonella enterica

characteristic: organism

text: Salmonella enterica


text: IZSLER

characteristic: INSDC center name

text: public

characteristic: collection date

text: Italy

text: not provided

text: Sus scrofa

text: environmental

text: Salmonella enterica

text: Salmonella enterica


text: IZSLER

text: public

characteristic: description

text: Italy

text: not provided

characteristic: host health state

text: not provided

text: not provided

text: Salmonella enterica

text: Salmonella enterica


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: human

characteristic: common name

text: Homo sapiens

text: Homo sapiens


characteristic: ENA-CHECKLIST

text: IZSLER

text: public

text: Italy

text: not provided

text: Anas platyrhynchos domesticus

characteristic: host scientific name

characteristic: isolate

text: environmental

text: Salmonella enterica

text: Salmonella enterica


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: IZSLER

text: public

text: Italy

characteristic: geographic location (country and/or sea)

text: not provided

text: homo sapiens

text: feces

text: Salmonella enterica

text: Salmonella enterica


text: IZSLER

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Italy

text: not provided

text: homo sapiens

text: blood

text: Salmonella enterica

text: Salmonella enterica

characteristic: title


text: National Health Research Institutes

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:National Health Research Institutes

characteristic: Submitter Id

text: DH5[alpha] with asymmetric loops

text: Escherichia coli DH5[alpha]

text: Escherichia coli DH5[alpha]


characteristic: External Id

text: National Health Research Institutes

characteristic: INSDC center name

text: public

text: DH5[alpha] with symmetric loops

text: Escherichia coli DH5[alpha]

characteristic: organism

text: Escherichia coli DH5[alpha]


text: National Health Research Institutes

text: public

text: ZymoBIOMICS standard

characteristic: description

text: synthetic metagenome

text: synthetic metagenome

text: ZymoBIOMICS standard


text: National Health Research Institutes

text: public

text: PacBio sequenced sample

text: human feces metagenome

text: human feces metagenome

text: PacBio sequenced sample


text: UNIVERSITY OF AMSTERDAM

text: public

text: S06_Elite_cultivar_typeVI_leaf_gland_trichome_rep1

tag: Namespace:UNIVERSITY OF AMSTERDAM

text: Rodrigo Therezan

characteristic: collection date

text: tomato

characteristic: common name

text: Elite cultivar

characteristic: genotype

text: Netherlands

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: glasshouse

characteristic: growth facility

characteristic: isolation and growth condition

text: Solanum lycopersicum

text: tomato

characteristic: organism common name

characteristic: plant developmental stage

text: soil

characteristic: plant growth medium

characteristic: plant structure

text: diploid

characteristic: ploidy

text: healthy

characteristic: sample health state

text: Solanum lycopersicum

text: active (highly productive in terpenes, large trichome cavity) and lazy (poorly productive, small cavity) trichomes study


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF AMSTERDAM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: S07_Elite_cultivar_typeVI_leaf_gland_trichome_rep2

characteristic: Submitter Id

text: Rodrigo Therezan

text: tomato

text: Elite cultivar

text: Netherlands

characteristic: geographic location (country and/or sea)

text: glasshouse

text: Solanum lycopersicum

text: tomato

text: soil

text: diploid

text: healthy

text: Solanum lycopersicum

characteristic: title


text: UNIVERSITY OF AMSTERDAM

characteristic: INSDC center name

text: public

text: S08_Elite_cultivar_typeVI_leaf_gland_trichome_rep3

text: Rodrigo Therezan

characteristic: collection date

text: tomato

characteristic: common name

characteristic: description

text: Elite cultivar

characteristic: genotype

text: Netherlands

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: glasshouse

characteristic: growth facility

characteristic: isolation and growth condition

text: Solanum lycopersicum

characteristic: organism

text: tomato

characteristic: organism common name

text: soil

text: diploid

text: healthy

text: Solanum lycopersicum


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF AMSTERDAM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: S09_Elite_cultivar_typeVI_leaf_gland_trichome_rep4

text: Rodrigo Therezan

text: tomato

text: Elite cultivar

text: Netherlands

text: glasshouse

text: Solanum lycopersicum

text: tomato

characteristic: plant developmental stage

text: soil

characteristic: plant growth medium

characteristic: plant structure

text: diploid

characteristic: ploidy

text: healthy

characteristic: sample health state

text: Solanum lycopersicum


text: UNIVERSITY OF AMSTERDAM

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: S10_Elite_cultivar_typeVI_leaf_gland_trichome_rep5

characteristic: Submitter Id

text: Rodrigo Therezan

characteristic: collection date

text: tomato

characteristic: common name

characteristic: description

text: Elite cultivar

characteristic: genotype

text: Netherlands

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: glasshouse

text: Solanum lycopersicum

text: tomato

text: soil

text: diploid

text: healthy

text: Solanum lycopersicum

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF AMSTERDAM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Rodrigo Therezan

text: F2-P28 S. lycopersicum Elite cultivar x S. habrochaites PI127826

text: Netherlands

characteristic: geographic location (longitude)

text: glasshouse

characteristic: growth facility

characteristic: isolation and growth condition

text: Solanum lycopersicum x Solanum habrochaites

characteristic: organism

text: tomato

characteristic: organism common name

text: soil

text: diploid

text: healthy


text: Leitat Technological Center

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Leitat Technological Center

characteristic: Submitter Id

text: Laboratory environment (ENVO_01001405)

characteristic: broad-scale environmental context

characteristic: collection date

text: biofilm

text: Biofilm material (ENVO:01000156)

characteristic: environmental medium

text: Spain

text: not collected

text: not collected

text: Laboratory environment (ENVO_01001405)

characteristic: local environmental context

text: metagenome

text: ARGs study in bioelectrochemical remediation

characteristic: project name

text: metagenome


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Leitat Technological Center

text: public

text: Laboratory environment (ENVO_01001405)

text: biomass

characteristic: description

text: Biofilm material (ENVO:01000156)

text: Spain

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: geographic location (latitude)

text: not collected

text: Laboratory environment (ENVO_01001405)

text: metagenome

text: metagenome

characteristic: title


text: INRAE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: INRAE

text: domestic pig

characteristic: common name

text: DNA

text: adult

text: Qiagen MagAttract High Molecular Weight

characteristic: extraction method

text: Iannuccelli N.

characteristic: host scientific name

text: GenPhySE

text: Sus scrofa domesticus

characteristic: organism

text: Sus scrofa domesticus

text: Male

characteristic: sex

text: Meishan

characteristic: strain

text: blood


characteristic: ENA-CHECKLIST

text: INRAE

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: INRAE

text: domestic pig

text: DNA

text: adult

text: Qiagen MagAttract High Molecular Weight

text: Iannuccelli N.

text: GenPhySE

text: Sus scrofa domesticus

text: Sus scrofa domesticus

text: Female

text: Meishan

text: blood


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRAE

text: public

text: INRAE

text: domestic pig

text: DNA

characteristic: description

text: adult

text: Qiagen MagAttract High Molecular Weight

text: Iannuccelli N.

text: GenPhySE

text: Sus scrofa domesticus

text: Sus scrofa domesticus

text: Female

text: LargeWhite

text: blood


text: INRAE

characteristic: INSDC center name

text: public

text: INRAE

text: domestic pig

text: DNA

text: 1 day

text: Qiagen MagAttract High Molecular Weight

characteristic: father

text: Iannuccelli N.

text: GenPhySE

characteristic: mother

text: Sus scrofa domesticus

text: Sus scrofa domesticus

text: Female

text: LargeWhiteMeishan

text: blood

characteristic: title


text: INRAE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: INRAE

text: domestic pig

characteristic: common name

text: DNA

text: 1 day

text: Qiagen MagAttract High Molecular Weight

characteristic: extraction method

text: Iannuccelli N.

text: GenPhySE

text: Sus scrofa domesticus

text: Sus scrofa domesticus

text: Male

text: LargeWhiteMeishan

text: blood


text: INRAE

text: public

text: INRAE

text: domestic pig

text: DNA

text: 1 day

text: Qiagen MagAttract High Molecular Weight

text: Iannuccelli N.

characteristic: host scientific name

text: GenPhySE

text: Sus scrofa domesticus

characteristic: organism

text: Sus scrofa domesticus

text: Female

characteristic: sex

text: MeishanLargeWhite

characteristic: strain

text: blood


characteristic: ENA-CHECKLIST

text: INRAE

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: INRAE

text: domestic pig

text: DNA

text: 1 day

text: Qiagen MagAttract High Molecular Weight

text: Iannuccelli N.

text: GenPhySE

text: Sus scrofa domesticus

text: Sus scrofa domesticus

text: Male

text: MeishanLargeWhite

text: blood


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRAE

text: public

text: commercial line

text: domestic pig

text: DNA

characteristic: description

text: adult

text: Qiagen MagAttract High Molecular Weight

text: Iannuccelli N.

text: commercial line

text: GenPhySE

text: Sus scrofa domesticus

text: Sus scrofa domesticus

text: Male

text: LargeWhite

text: blood


text: INRAE

characteristic: INSDC center name

text: public

text: INRAE

text: domestic pig

text: DNA

text: adult

text: Qiagen Tip100

text: Iannuccelli N.

text: GenPhySE

text: Sus scrofa domesticus

text: Sus scrofa domesticus

text: Male

text: Meishan

text: blood

characteristic: title


text: WEIZMANN INSTITUTE OF SCIENCE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: human

text: Homo sapiens

text: Homo sapiens


text: INRAE

text: public

text: INRAE

text: domestic pig

characteristic: common name

text: DNA

text: adult

text: Qiagen Tip100

characteristic: extraction method

text: Iannuccelli N.

text: GenPhySE

text: Sus scrofa domesticus

text: Sus scrofa domesticus

text: Female

text: Meishan

text: blood


text: INRAE

text: public

text: INRAE

text: domestic pig

text: DNA

text: adult

text: Qiagen Tip100

text: Iannuccelli N.

characteristic: host scientific name

text: GenPhySE

text: Sus scrofa domesticus

characteristic: organism

text: Sus scrofa domesticus

text: Female

text: LargeWhite

text: blood


text: INRAE

text: public

text: INRAE

text: domestic pig

text: DNA

text: 1 day

text: Qiagen Tip100

characteristic: father

text: Iannuccelli N.

text: GenPhySE

characteristic: mother

text: Sus scrofa domesticus

text: Sus scrofa domesticus

text: Female

characteristic: sex

text: LargeWhiteMeishan

characteristic: strain

text: blood


characteristic: ENA-CHECKLIST

text: INRAE

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: INRAE

text: domestic pig

text: DNA

text: 1 day

text: Qiagen Tip100

text: Iannuccelli N.

text: GenPhySE

text: Sus scrofa domesticus

text: Sus scrofa domesticus

text: Male

text: LargeWhiteMeishan

text: blood


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRAE

text: public

text: INRAE

text: domestic pig

text: DNA

characteristic: description

text: 1 day

text: Qiagen Tip100

text: Iannuccelli N.

text: GenPhySE

text: Sus scrofa domesticus

text: Sus scrofa domesticus

text: Female

text: MeishanLargeWhite

text: blood


text: INRAE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: INRAE

text: domestic pig

text: DNA

text: 1 day

text: Qiagen Tip100

text: Iannuccelli N.

text: GenPhySE

text: Sus scrofa domesticus

text: Sus scrofa domesticus

text: Male

text: MeishanLargeWhite

text: blood

characteristic: title


text: INRAE

text: public

text: INRAE

text: domestic pig

characteristic: common name

text: DNA

text: 1 day

characteristic: extraction method

text: Iannuccelli N.

characteristic: host scientific name

text: GenPhySE

text: Sus scrofa domesticus

characteristic: organism

text: Sus scrofa domesticus

text: Male

text: MeishanLargeWhite

text: blood


text: INRAE

text: public

text: commercial line

text: domestic pig

text: DNA

text: adult

text: phenol chloroform home-made protocol

text: Iannuccelli N.

text: commercial line

text: GenPhySE

text: Sus scrofa domesticus

text: Sus scrofa domesticus

text: Male

characteristic: sex

text: LargeWhite

characteristic: strain

text: sperm


ARGFX

characteristic: ENA-CHECKLIST

text: UNIVERSITY OF OULU

text: public

characteristic: INSDC status

text: ARGFX

tag: Namespace:UNIVERSITY OF OULU

characteristic: broad-scale environmental context

characteristic: collection date

text: human

text: ARGFX

characteristic: environmental medium

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: local environmental context

text: Homo sapiens

text: ChIP-seq data of PRDL factors in HEK293 cells

characteristic: project name

text: Homo sapiens

text: ARGFX


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF OULU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: human

text: ARGFX processed

characteristic: description

text: Finland

text: Homo sapiens

text: Homo sapiens

characteristic: title


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF OULU

text: public

characteristic: INSDC status

characteristic: broad-scale environmental context

characteristic: collection date

text: human

characteristic: common name

text: Finland

text: Homo sapiens

characteristic: organism

text: Homo sapiens


text: UNIVERSITY OF OULU

text: public

text: human

text: CPHX1 processed

characteristic: environmental medium

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: local environmental context

text: Homo sapiens

characteristic: project name

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF OULU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: human

text: CPHX2 processed

text: Finland

text: Homo sapiens

text: Homo sapiens


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF OULU

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: human

text: CPHX2-ARGFX_Input processed

characteristic: description

text: Finland

text: Homo sapiens

text: Homo sapiens

characteristic: title


CRX

text: UNIVERSITY OF OULU

text: public

text: CRX

characteristic: broad-scale environmental context

characteristic: collection date

text: human

characteristic: common name

text: CRX

characteristic: environmental medium

text: Finland

characteristic: geographic location (country and/or sea)

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: CRX


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF OULU

text: public

text: human

text: CRX processed

text: Finland

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: local environmental context

text: Homo sapiens

characteristic: project name

text: Homo sapiens


characteristic: ENA-CHECKLIST

characteristic: External Id

text: UNIVERSITY OF OULU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: human

text: CRX-EGR2-DUX4-ZNF263_Input processed

text: Finland

text: Homo sapiens

text: Homo sapiens


DPRX

text: UNIVERSITY OF OULU

text: public

characteristic: SRA accession

text: DPRX

characteristic: Submitter Id

characteristic: broad-scale environmental context

characteristic: collection date

text: human

text: DPRX

characteristic: description

text: Finland

text: Homo sapiens

text: Homo sapiens

text: DPRX

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF OULU

text: public

text: human

characteristic: common name

text: DPRX processed

characteristic: environmental medium

text: Finland

characteristic: geographic location (country and/or sea)

text: Homo sapiens

characteristic: organism

text: Homo sapiens


characteristic: ENA-CHECKLIST

characteristic: External Id

text: UNIVERSITY OF OULU

text: public

text: human

text: DUX4 processed

text: Finland

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: local environmental context

text: Homo sapiens

characteristic: project name

text: Homo sapiens


text: UNIVERSITY OF OULU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: human

text: DUX4-TPRX2-CPHX1_Input processed

text: Finland

text: Homo sapiens

text: Homo sapiens


DUXA

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF OULU

text: public

text: DUXA

characteristic: broad-scale environmental context

characteristic: collection date

text: human

text: DUXA

characteristic: description

text: Finland

text: Homo sapiens

text: Homo sapiens

text: DUXA

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: human

characteristic: common name

text: Homo sapiens

characteristic: organism

text: Homo sapiens


text: UNIVERSITY OF OULU

text: public

text: human

text: DUXA processed

characteristic: environmental medium

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: local environmental context

text: Homo sapiens

characteristic: project name

text: Homo sapiens


DUXB

text: UNIVERSITY OF OULU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: DUXB

characteristic: Submitter Id

text: human

text: DUXB

text: Finland

text: Homo sapiens

text: Homo sapiens

text: DUXB


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF OULU

text: public

characteristic: broad-scale environmental context

characteristic: collection date

text: human

text: DUXB processed

characteristic: description

text: Finland

text: Homo sapiens

text: Homo sapiens

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

text: UNIVERSITY OF OULU

text: public

text: human

characteristic: common name

text: EGR2 processed

characteristic: environmental medium

text: Finland

characteristic: geographic location (country and/or sea)

text: Homo sapiens

characteristic: organism

text: Homo sapiens


text: UNIVERSITY OF OULU

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: human

text: Finland

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: local environmental context

text: Homo sapiens

characteristic: project name

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF OULU

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: human

text: KLF3 processed

text: Finland

text: Homo sapiens

text: Homo sapiens


characteristic: ENA-CHECKLIST

characteristic: External Id

text: UNIVERSITY OF OULU

text: public

characteristic: broad-scale environmental context

characteristic: collection date

text: human

text: KLF3-DPRX-DUB-TPRX1_Input processed

characteristic: description

text: Finland

text: Homo sapiens

text: Homo sapiens

characteristic: title


text: UNIVERSITY OF OULU

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: human

characteristic: common name

text: TPRX1 processed

characteristic: environmental medium

text: Finland

characteristic: geographic location (country and/or sea)

text: Homo sapiens

characteristic: organism

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF OULU

text: public

text: human

text: TPRX2 processed

text: Finland

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: local environmental context

text: Homo sapiens

characteristic: project name

text: Homo sapiens


characteristic: ENA-CHECKLIST

characteristic: External Id

text: UNIVERSITY OF OULU

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: human

text: ZNF263 processed

text: Finland

text: Homo sapiens

text: Homo sapiens


text: EMBL

characteristic: INSDC center name

characteristic: INSDC first public

text: public

unit: week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: spermatid

text: house mouse

text: Protocols: The genotype for KO is homozygous for the mutation "FVB/NCrl-Trim66em2(gfp)Emr". The homozygous mutants were produced by intercrossing heterozygous for the allele FVB/NCrl-Trim66em2(gfp)Emr. The allele FVB/NCrl-Trim66em2(gfp)Emr was created at EMBL. Spermatids were isolated from testes by fluorescent activated cell sorting (FACS) using a BD FACS ariaII (BD Biosciences). The material was sourced from both testes for each mouse and was run until the whole material was sorted. The dissected tissue was incubated in enhanced Krebs-Ringer's buffer (120 mM NaCl, 4.8 mM KCl, 25.2 mM NaHCO3, 1.2 mM KH2PO3, 1.2 mM Mg2SO4, 1.3 mM CaCl2, 11mM glucose, 1X Non-essential AA (Gibco), Pen/Strep 1X (Gibco)). De-encapsulated testes were digested for 5 min in 0.5 mg/ml collagenase (Clostridium histolyticum, Sigma), then treated for 10 min with Trypsin (Acetylated from bovine pancreas, type V-S) at 32 °C. The staining was done in EKRB buffer with 10% FBS, with 10ug/ml Hoechst 33342 for 30 min and 10 min with 2 µg/ml propidium iodide at 32 °C. The cells were sorted in a sorting buffer (10% FBS enhanced Krebs-Ringer's buffer). The sorted spermatid fractions were collected in Monarch DNA/RNA protection reagent (NEB) and flash frozen in liquid nitrogen before the RNA extraction. The animals were sacrificed by cervical dislocation. The testis were extracted from the abdominal cavity pulling the fat pads surroundings tissue. The epidyndymis was surgically detached and excess of fat removed in suspension of 1x PBS. The tissue was washed once in 1x PBS before the de-encapsulation of the testis tubules. After removal of the tunica the testis were placed in EKRB buffer before the enzymatic tissue dissociation. Total RNA was extracted from collected spermatids fractions with Monarch RNA extraction kit (NEB) according to manufacturers' protocol for RNA extraction from tissue or leukocytes, with proteinase K incubation time optimised for homogenized tissues. The optional step with DNAseI treatment was performed plus an additional steps with TurboDNase to eliminate all traces of genomic DNA (Invitrogen). Total RNA was then purified with Monarch RNA Cleanup Kit (NEB) and quantified using Qubit Fluorimetric Quantification (Thermo Scientific). The integrity of the RNA was determined using the TapeStation High Sensitivity RNA kit (Agilent Technologies). 10 ng of total RNA has been used to prepare ribo-depleted RNA-seq libraries with these kits: NEBNext rRNA Depletion Kit (Human/Mouse/Rat) and NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®. We used 17 PCR cycles to obtain enough material for sequencing.

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

characteristic: individual

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: testis

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex

text: FVB

characteristic: strain

characteristic: title

unit: nanogram per microliter

characteristic: total RNA


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMBL

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: spermatid

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: testis

text: Mus musculus

text: male

text: FVB


text: KYUNG HEE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

tag: Namespace:KYUNG HEE UNIVERSITY

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: Oc cell

text: protoplast

text: Japanese rice

characteristic: cultivar

text: Protocols: Rice Oc cell line was established from seedling roots of Oryza sativa L. indica-type accession C5928 (Baba et al. 1986 1). The cell suspension culture is fast growing and composed of small and near-uniform clumps of cells. The Oc cells are grown in a Murashige and Skoog (MS) medium supplemented with 1 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D), pH 5.7. Our Oc cell culture has been maintained in the dark at 27°C with rotary shaking at 120 rpm and subcultured at 7-day intervals. DOI: 10.1093/oxfordjournals.pcp.a077122. Oc cells were grown for 10 days in an R2S solution complemented with 3% glucose as a carbon source. They were divided into two equal parts; one was added with sucrose and the other with glucose, obtaining a final sugar concentration of 3% in two replications. Samples were collected immediately before the transfer to new media (0 h) and 4 hours after sugar treatment (4 h). Disrupt a maximum of 100 mg plant material in liquid nitrogen and add 450 µl Buffer RLT. Vortex vigorously. Transfer the lysate to a QIAshredder spin column (lilac) placed in a 2 ml collection tube. Centrifuge for 2 min at full speed. Transfer the supernatant of the flow-through to a new microcentrifuge tube without disturbing the cell-debris pellet. Add 0.5 volume of ethanol (96–100%) to the cleared lysate, and mix immediately by pipetting. Transfer the sample (usually 650 μl), with any precipitate, to an RNeasy Mini spin column in a 2 ml collection tube. Close the lid, and centrifuge for 15 s at ≥8000 x g. Discard the flow-through. Add 700 µl Buffer RW1 to the RNeasy spin column. Close the lid, and centrifuge for 15 s at ≥8000 x g. Discard the flow-through. Add 500 µl Buffer RPE to the RNeasy spin column. Close the lid, and centrifuge for 15 s at ≥8000 x g. Discard the flow-through. 8. Add 500 µl Buffer RPE to the RNeasy spin column. Close the lid, and centrifuge for 2 min at ≥8000 x g. Centrifuge at full speed for 1 min to dry the membrane. Place the RNeasy spin column in a new 1.5 ml collection tube. Add 30–50 µl RNase-free water directly to the spin column membrane. Close the lid, and centrifuge for 1 min at ≥8000 x g to elute the RNA. The sequencing library is prepared by random fragmentation of the DNA or cDNA sample, followed by 5' and 3' adapter ligation. Alternatively, \"tagmentation\" combines the fragmentation and ligation reactions into a single step that greatly increases the efficiency of the library preparation process. Adapter-ligated fragments are then PCR amplified and gel purified.

characteristic: description

text: normal

characteristic: disease

text: wild type genotype

text: not applicable

text: Oryza sativa Japonica Group

text: Oryza sativa Japonica Group

text: root

text: Oryza sativa Japonica Group


Empty

text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: Empty

text: human

text: Homo sapiens

text: Homo sapiens


text: Estacion Biologica de Donana, CSIC

text: public

tag: Namespace:Estacion Biologica de Donana, CSIC

text: Cleia Detri, Uniarq

text: Spanish lynx

text: Mandible

text: Portugal

characteristic: geographic location (country and/or sea)

text: Monte Moliao, Lagos

characteristic: geographic location (region and locality)

text: Archeological site

text: Lynx pardinus

text: Lynx pardinus

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Estacion Biologica de Donana, CSIC

text: public

text: Jordi Nadal, SERP, U. Barcelona

text: Spanish lynx

text: Mandible

text: Spain

text: La Moleta del Remei, Alcanar

text: Archeological site

text: Lynx pardinus

text: Lynx pardinus


characteristic: External Id

text: Estacion Biologica de Donana, CSIC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Francisco Nocete, MIDAS, U Huelva

text: Spanish lynx

text: Bone

text: Spain

text: Eras del Alcazar, Úbeda

text: Archeological site

text: Lynx pardinus

text: Lynx pardinus


text: WEIZMANN INSTITUTE OF SCIENCE

characteristic: INSDC center name

text: public

text: human

characteristic: common name

text: Homo sapiens

characteristic: organism

text: Homo sapiens


IDAN

text: WEIZMANN INSTITUTE OF SCIENCE

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: IDAN

characteristic: Submitter Id

text: human

text: Homo sapiens

text: Homo sapiens


NC

text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: NC

text: human

text: Homo sapiens

text: Homo sapiens


PC

text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: PC

text: human

text: Homo sapiens

text: Homo sapiens


text: Hebrew University, Genetics Department

text: public

tag: Namespace:Hebrew University, Genetics Department

text: ArrayExpress

characteristic: broker name

text: neuron

text: human

text: Protocols: hESCs were grown on feeder layer MEFs combined with hESC medium until 50-60% confluency (small colonies). Cells were washed with PBS and gently dissociated using 0.25% Trypsin Solution A (Biological Industries) for approximately 5 minutes at 37Â°đś. Dissociated colonies were collected and transferred to non-adherent 10 cm dishes and suspended in confluency). The following day, mTeSR medium was replaced with embryoid body (EB) medium which contains same ingredients as standard hESC medium without bFGF (knockout Dulbeccoâs modified Eagleâs medium (DMEM) supplemented with 15% knockout serum replacement (KSR, Thermo Fisher Scientific), 0.1 mM nonessential amino acids, 2mM L-glutamine, 50 đđ đđ -1 streptomycin, 50 đ˘đđđĄđ  đđ-1 penicillin and 0.1 mM Î˛ mercaptoethanol), for 4 days. After 4 days EB medium was supplemented with 0.1 đM Retinoic Acid (RA, Sigma). Cells were grown in suspension for 17 more days before being plated on 10 cm Matrigel-coated dishes. Cells were grown on Matrigel up to 10 more days before they were collected for downstream analysis. Medium was changed every other day throughout the experiment. For sample collection, cells were washed with PBS and trypsinized. Qiagen RNeasy Kit KAPA Stranded mRNA-Seq Kit

characteristic: description

text: embryo

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characteristic: genotype

text: 3D culture

characteristic: growth condition

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens


characteristic: ENA-CHECKLIST

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characteristic: External Id

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text: embryo

text: PHLDA2 -/- / SLC22A18 -/-

text: 3D culture

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: embryonic stem cell

text: Homo sapiens

characteristic: title


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text: Homo sapiens

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text: ArrayExpress

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text: not applicable

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