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characteristic: broker name

text: house mouse

text: Protocols: The mice were sacrificed on different days post SARS-CoV-2 infection. Each uninfected mice tissue sample (control) was collected on the 6th-day post-infection of corresponding SARS CoV2 samples. Lung tissues were harvested in TRIzol. The tissues were homogenized in TRIzol and RNA was purified using RNeasy mini kit (QIAGEN #74104). The total RNA was extracted from uninfected and SARS-CoV-2 infected mice lung tissue using RNeasy mini kit (QIAGEN #74104). The quantity of RNA was determined using Qubit RNA assay kit with Qubit 4.0 and the quality of RNA was tested using agarose gel electrophoresis and High Sensitivity Tape station Kit (Agilent 2200, #5067-5576, #5067-5577 and #5067-5578). After assessing the quality of RNA, ~900 ng of total RNA was taken for library preparation using NEBNext®Ultra™ II Directional RNA Library kit for Illumina (# E7760L) and NEBNext Poly (A) mRNA Magnetic Isolation Module (# E7490L) as per manufacturer's protocol. The prepared library was quantified using Qubit dsDNA assay kit (Invitrogen, Q32851) followed by quality check (QC) and fragment size distribution using a High Sensitivity Tape station Kit (Agilent 2200, #5067-5584 and #5067-5585). After assessing the quality of RNA, 800-900 ng of total RNA was subjected to NEBNext® ultra™ directional RNA library prep kit for Illumina® (#E7420) using NEBNextPoly(A) mRNA magnetic isolation module (NEB #E7490). The QC of the prepared library was performed using High-Sensitivity Tape Station Kit (Agilent 2200, 5067-5585 (reagents) #5067-5584 ( screen tapes)) for fragment length distribution and Qubit dsDNA HS assay kit (Invitrogen, Q32851) for quantifying the library.

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

characteristic: individual

characteristic: infect

text: not applicable

characteristic: isolate

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lung

characteristic: organism part

text: Mus musculus

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CSIR-Centre For Cellular And Molecular Biology (CCMB)

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 8 to 10

text: ArrayExpress

text: house mouse

characteristic: common name

text: adult

text: not applicable

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus


characteristic: INSDC center name

text: public

text: E-MTAB-12681:K18-hACE2 + SARS-CoV-2_6DPI_1

text: 8 to 10

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: K18-hACE2 + SARS-CoV-2_6DPI_1

characteristic: individual

text: not applicable

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

text: K18-hACE2 + SARS-CoV-2_6DPI_1


characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-12681:K18-hACE2 + SARS-CoV-2_6DPI_2

text: 8 to 10

text: ArrayExpress

text: house mouse

text: adult

text: K18-hACE2 + SARS-CoV-2_6DPI_2

characteristic: infect

text: not applicable

characteristic: isolate

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lung

characteristic: organism part

text: Mus musculus

characteristic: strain

text: K18-hACE2 + SARS-CoV-2_6DPI_2

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12681:K18-hACE2 + SARS-CoV-2_6DPI_3

text: 8 to 10

text: ArrayExpress

text: house mouse

text: adult

text: K18-hACE2 + SARS-CoV-2_6DPI_3

text: not applicable

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

text: K18-hACE2 + SARS-CoV-2_6DPI_3


characteristic: INSDC center name

text: public

text: E-MTAB-12681:K18-hACE2 + SARS-CoV-2_7DPI_1

characteristic: Submitter Id

text: 8 to 10

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: K18-hACE2 + SARS-CoV-2_7DPI_1

text: not applicable

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

text: K18-hACE2 + SARS-CoV-2_7DPI_1


characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-12681:K18-hACE2 + SARS-CoV-2_7DPI_2

text: 8 to 10

text: ArrayExpress

text: house mouse

text: adult

characteristic: genotype

text: K18-hACE2 + SARS-CoV-2_7DPI_2

characteristic: individual

characteristic: infect

text: not applicable

characteristic: isolate

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lung

characteristic: organism part

text: Mus musculus

text: K18-hACE2 + SARS-CoV-2_7DPI_2


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-12681:K18-hACE2 + SARS-CoV-2_7DPI_3

text: 8 to 10

text: ArrayExpress

text: house mouse

text: adult

text: K18-hACE2 + SARS-CoV-2_7DPI_3

text: not applicable

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

characteristic: strain

text: K18-hACE2 + SARS-CoV-2_7DPI_3

characteristic: title


characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12681:K18-hACE2 Ifnar1-/- Uninfected_1

characteristic: Submitter Id

text: 8 to 10

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: adult

text: K18-hACE2 Ifnar1 knock-out

text: K18-hACE2 Ifnar1-/- Uninfected_1

text: not applicable

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

text: K18-hACE2 Ifnar1-/- Uninfected_1


characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-12681:K18-hACE2 Ifnar1-/- Uninfected_2

text: 8 to 10

text: ArrayExpress

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: K18-hACE2 Ifnar1 knock-out

characteristic: genotype

text: K18-hACE2 Ifnar1-/- Uninfected_2

characteristic: individual

characteristic: infect

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

text: K18-hACE2 Ifnar1-/- Uninfected_2


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-12681:K18-hACE2 Ifnar1-/- Uninfected_3

text: 8 to 10

text: ArrayExpress

text: house mouse

text: adult

text: K18-hACE2 Ifnar1 knock-out

text: K18-hACE2 Ifnar1-/- Uninfected_3

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lung

characteristic: organism part

text: Mus musculus

text: K18-hACE2 Ifnar1-/- Uninfected_3


name: E-MTAB-12681:K18-hACE2 Ifnar1-/- + SARS-CoV-2_6DPI_1

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 8 to 10

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: K18-hACE2 Ifnar1 knock-out

text: K18-hACE2 Ifnar1-/- + SARS-CoV-2_6DPI_1

text: not applicable

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

characteristic: strain

text: K18-hACE2 Ifnar1-/- + SARS-CoV-2_6DPI_1

characteristic: title


name: E-MTAB-12681:K18-hACE2 Ifnar1-/- + SARS-CoV-2_6DPI_2

characteristic: ENA-CHECKLIST

text: public

text: 8 to 10

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: K18-hACE2 Ifnar1 knock-out

characteristic: genotype

text: K18-hACE2 Ifnar1-/- + SARS-CoV-2_6DPI_2

characteristic: individual

text: not applicable

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

text: K18-hACE2 Ifnar1-/- + SARS-CoV-2_6DPI_2


name: E-MTAB-12681:K18-hACE2 Ifnar1-/- + SARS-CoV-2_6DPI_3

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: 8 to 10

text: ArrayExpress

text: house mouse

text: adult

text: K18-hACE2 Ifnar1 knock-out

text: K18-hACE2 Ifnar1-/- + SARS-CoV-2_6DPI_3

characteristic: infect

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

text: K18-hACE2 Ifnar1-/- + SARS-CoV-2_6DPI_3


name: E-MTAB-12681:K18-hACE2 Ifnar1-/- + SARS-CoV-2_7DPI_1

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 8 to 10

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: K18-hACE2 Ifnar1 knock-out

text: K18-hACE2 Ifnar1-/- + SARS-CoV-2_7DPI_1

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lung

characteristic: organism part

text: Mus musculus

text: K18-hACE2 Ifnar1-/- + SARS-CoV-2_7DPI_1


name: E-MTAB-12681:K18-hACE2 Ifnar1-/- + SARS-CoV-2_7DPI_2

characteristic: ENA-CHECKLIST

text: public

text: 8 to 10

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: K18-hACE2 Ifnar1 knock-out

text: K18-hACE2 Ifnar1-/- + SARS-CoV-2_7DPI_2

text: not applicable

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

characteristic: strain

text: K18-hACE2 Ifnar1-/- + SARS-CoV-2_7DPI_2

characteristic: title


name: E-MTAB-12681:K18-hACE2 Ifnar1-/- + SARS-CoV-2_7DPI_3

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: 8 to 10

text: ArrayExpress

text: house mouse

text: adult

text: K18-hACE2 Ifnar1 knock-out

characteristic: genotype

text: K18-hACE2 Ifnar1-/- + SARS-CoV-2_7DPI_3

characteristic: individual

text: not applicable

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

text: K18-hACE2 Ifnar1-/- + SARS-CoV-2_7DPI_3


characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12681:K18-hACE2 Irgm1-/- Uninfected_1

characteristic: Submitter Id

text: 8 to 10

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: K18-hACE2 Irgm1 knock-out

text: K18-hACE2 Irgm1-/- Uninfected_1

characteristic: infect

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

text: K18-hACE2 Irgm1-/- Uninfected_1


characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-12681:K18-hACE2 Irgm1-/- Uninfected_2

text: 8 to 10

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: K18-hACE2 Irgm1 knock-out

text: K18-hACE2 Irgm1-/- Uninfected_2

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lung

characteristic: organism part

text: Mus musculus

text: K18-hACE2 Irgm1-/- Uninfected_2


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-12681:K18-hACE2 Irgm1-/- Uninfected_3

text: 8 to 10

text: ArrayExpress

text: house mouse

text: adult

text: K18-hACE2 Irgm1 knock-out

text: K18-hACE2 Irgm1-/- Uninfected_3

text: not applicable

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

characteristic: strain

text: K18-hACE2 Irgm1-/- Uninfected_3

characteristic: title


name: E-MTAB-12681:K18-hACE2 Irgm1-/- + SARS-CoV-2_6DPI_1

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 8 to 10

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: K18-hACE2 Irgm1 knock-out

characteristic: genotype

text: K18-hACE2 Irgm1-/- + SARS-CoV-2_6DPI_1

characteristic: individual

text: not applicable

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

text: K18-hACE2 Irgm1-/- + SARS-CoV-2_6DPI_1


name: E-MTAB-12681:K18-hACE2 Irgm1-/- + SARS-CoV-2_6DPI_2

characteristic: ENA-CHECKLIST

text: public

text: 8 to 10

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: K18-hACE2 Irgm1 knock-out

text: K18-hACE2 Irgm1-/- + SARS-CoV-2_6DPI_2

characteristic: infect

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

text: K18-hACE2 Irgm1-/- + SARS-CoV-2_6DPI_2


name: E-MTAB-12681:K18-hACE2 Irgm1-/- + SARS-CoV-2_6DPI_3

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: 8 to 10

text: ArrayExpress

text: house mouse

text: adult

text: K18-hACE2 Irgm1 knock-out

text: K18-hACE2 Irgm1-/- + SARS-CoV-2_6DPI_3

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lung

characteristic: organism part

text: Mus musculus

text: K18-hACE2 Irgm1-/- + SARS-CoV-2_6DPI_3


text: Duke University

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: RIN

characteristic: SRA accession

tag: Namespace:Duke University

characteristic: Submitter Id

unit: months

characteristic: age

text: house mouse

text: Young male APOE2 mouse blood sample 10

text: mouse blood

text: Mus musculus

text: Mus musculus

text: male

characteristic: sex

characteristic: title


characteristic: ENA-CHECKLIST

text: Duke University

text: public

text: house mouse

characteristic: common name

text: Young male APOE2 mouse blood sample 5

characteristic: description

text: mouse blood

text: Mus musculus

text: Mus musculus

text: male


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Duke University

text: public

text: house mouse

text: Young male APOE2 mouse blood sample 6

text: mouse blood

text: Mus musculus

text: Mus musculus

text: male


characteristic: External Id

text: Duke University

characteristic: INSDC first public

text: public

text: house mouse

text: Young female APOE2 mouse blood sample 7

text: mouse blood

text: Mus musculus

text: Mus musculus

text: female


text: Duke University

characteristic: INSDC last update

text: public

text: house mouse

text: Young female APOE2 mouse blood sample 8

text: mouse blood

text: Mus musculus

characteristic: organism

text: Mus musculus

text: female


text: Duke University

characteristic: INSDC center name

text: public

text: house mouse

text: Young male APOE2 mouse blood sample 9

text: mouse blood

text: Mus musculus

text: Mus musculus

text: male


text: Duke University

text: public

characteristic: INSDC status

text: house mouse

text: Old male APOE2 mouse blood sample 10

text: mouse blood

text: Mus musculus

text: Mus musculus

text: male


text: Duke University

text: public

characteristic: RIN

text: house mouse

text: Old male APOE2 mouse blood sample 11

text: mouse blood

text: Mus musculus

text: Mus musculus

text: male


text: Duke University

text: public

characteristic: SRA accession

text: house mouse

text: Old male APOE2 mouse blood sample 12

text: mouse blood

text: Mus musculus

text: Mus musculus

text: male


text: Duke University

text: public

characteristic: Submitter Id

text: house mouse

text: Old male APOE2 mouse blood sample 3

text: mouse blood

text: Mus musculus

text: Mus musculus

text: male


text: Duke University

text: public

characteristic: age

text: house mouse

text: Old male APOE2 mouse blood sample 4

text: mouse blood

text: Mus musculus

text: Mus musculus

text: male


text: Duke University

text: public

text: house mouse

text: Old male APOE2 mouse blood sample 5

text: mouse blood

text: Mus musculus

text: Mus musculus

text: male

characteristic: sex


text: Duke University

text: public

text: house mouse

text: Old female APOE2 mouse blood sample 6

text: mouse blood

text: Mus musculus

text: Mus musculus

text: female

characteristic: title


characteristic: ENA-CHECKLIST

text: Duke University

text: public

text: house mouse

text: Old female APOE2 mouse blood sample 7

text: mouse blood

text: Mus musculus

text: Mus musculus

text: female


text: Duke University

text: public

text: house mouse

characteristic: common name

text: Old female APOE2 mouse blood sample 8

text: mouse blood

text: Mus musculus

text: Mus musculus

text: female


text: Duke University

text: public

text: house mouse

text: Old female APOE2 mouse blood sample 9

characteristic: description

text: mouse blood

text: Mus musculus

text: Mus musculus

text: female


characteristic: ENA-FIRST-PUBLIC

text: Duke University

text: public

text: house mouse

text: Old female APOE2 mouse blood sample 10

text: mouse blood

text: Mus musculus

text: Mus musculus

text: female


characteristic: ENA-LAST-UPDATE

text: Duke University

text: public

text: house mouse

text: Old male APOE2 mouse blood sample 13

text: mouse blood

text: Mus musculus

text: Mus musculus

text: male


characteristic: External Id

text: Duke University

text: public

text: house mouse

text: Old female APOE2 mouse blood sample 1

text: mouse blood

text: Mus musculus

text: Mus musculus

text: female


text: Duke University

characteristic: INSDC first public

text: public

text: house mouse

text: Old female APOE2 mouse blood sample 2

text: mouse blood

text: Mus musculus

text: Mus musculus

text: female


text: Duke University

characteristic: INSDC last update

text: public

text: house mouse

text: Old female APOE2 mouse blood sample 3

text: mouse blood

text: Mus musculus

text: Mus musculus

text: female


text: Duke University

text: public

text: house mouse

text: Old female APOE2 mouse blood sample 4

text: mouse blood

text: Mus musculus

characteristic: organism

text: Mus musculus

text: female


text: Duke University

characteristic: INSDC center name

text: public

text: house mouse

text: Old female APOE2 mouse blood sample 5

text: mouse blood

text: Mus musculus

text: Mus musculus

text: female


text: University of Neuchatel

text: public

characteristic: INSDC status

tag: Namespace:University of Neuchatel

unit: m

characteristic: altitude

text: temperate mixed forest biome [ENVO:01000212]

characteristic: broad-scale environmental context

characteristic: collection date

text: pool of ten subsample of 100 g and sieved at 2 mm mesh size

characteristic: composite design/sieving (if any)

characteristic: depth

text: CH-01-FO-SP1 bulk soil

characteristic: elevation

text: soil [ENVO:00001998]|plant litter [ENVO:01000628]

characteristic: environmental medium

text: Switzerland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Salgesch, Valais

characteristic: geographic location (region and locality)

text: soil surface layer [ENVO:04000010]|litter layer [ENVO:01000338]

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: pH

text: five grams of dry soil mixed in 12.5 ml of distilled water

characteristic: pH method

text: initial denaturation=95degC_5min; 35 cycles:(denaturation=94degC_30sec; annealing=47degC_45sec; elongation=72degC_1min); final elongation=72degC_10min

characteristic: pcr conditions

text: TAReuk454FWD1:CCAGCASCYGCGGTAATTCC; TAReukREV3:ACTTTCGTTCTTGATYRA

characteristic: pcr primers

text: Soil protists spatio-temporal turnover in mountains

characteristic: project name

text: soil sieving

characteristic: sample material processing

unit: g

characteristic: sample weight for DNA extraction

text: uncultured eukaryote

text: MiSeq

characteristic: sequencing method

characteristic: slope aspect

characteristic: slope gradient

text: in LifeGuard® (Qiagen) at -20degC

characteristic: storage conditions (fresh/frozen/other)

characteristic: target gene

characteristic: target subfragment

text: Thermo ScientificTM FLASH 2000 CHNS elemental analyzer

characteristic: total organic C method

unit: g/kg

characteristic: total organic carbon

unit: g/g

characteristic: water content

text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: CH-01-FO-SP2 bulk soil

characteristic: description

characteristic: elevation

characteristic: environmental medium

text: Switzerland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Miège, Valais

characteristic: geographic location (region and locality)

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: organism

characteristic: pH

text: soil sieving

text: uncultured eukaryote

text: MiSeq

text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

text: CH-01-FO-SP3 bulk soil

text: Switzerland

text: Salgesch, Valais

text: uncultured eukaryote

characteristic: pH method

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: soil sieving

characteristic: sample material processing

characteristic: sample weight for DNA extraction

text: uncultured eukaryote

text: MiSeq

characteristic: sequencing method

characteristic: slope aspect

characteristic: slope gradient

text: in LifeGuard® (Qiagen) at -20degC

characteristic: storage conditions (fresh/frozen/other)

characteristic: target gene

characteristic: target subfragment

characteristic: total organic C method

characteristic: total organic carbon

characteristic: water content

text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: CH-01-FO-SP4 bulk soil

characteristic: description

characteristic: elevation

characteristic: environmental medium

text: Switzerland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Crans-Montana, Valais

characteristic: geographic location (region and locality)

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: organism

characteristic: pH

text: soil sieving

text: uncultured eukaryote

text: MiSeq

text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

text: CH-01-FO-SP5 bulk soil

text: Switzerland

text: Crans-Montana, Valais

text: uncultured eukaryote

characteristic: pH method

characteristic: pcr conditions

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characteristic: project name

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characteristic: sample material processing

characteristic: sample weight for DNA extraction

text: uncultured eukaryote

text: MiSeq

characteristic: sequencing method

characteristic: slope aspect

characteristic: slope gradient

text: in LifeGuard® (Qiagen) at -20degC

characteristic: storage conditions (fresh/frozen/other)

characteristic: target gene

characteristic: target subfragment

characteristic: total organic C method

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characteristic: water content

text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

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characteristic: INSDC center name

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characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: CH-01-FO-SU1 bulk soil

characteristic: description

characteristic: elevation

characteristic: environmental medium

text: Switzerland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Salgesch, Valais

characteristic: geographic location (region and locality)

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: organism

characteristic: pH

text: soil sieving

text: uncultured eukaryote

text: MiSeq

text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

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characteristic: INSDC center name

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text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

text: CH-01-FO-SU2 bulk soil

text: Switzerland

text: Miège, Valais

text: uncultured eukaryote

characteristic: pH method

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: soil sieving

characteristic: sample material processing

characteristic: sample weight for DNA extraction

text: uncultured eukaryote

text: MiSeq

characteristic: sequencing method

characteristic: slope aspect

characteristic: slope gradient

text: in LifeGuard® (Qiagen) at -20degC

characteristic: storage conditions (fresh/frozen/other)

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characteristic: target subfragment

characteristic: total organic C method

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characteristic: water content

text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

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characteristic: INSDC center name

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characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: CH-01-FO-SU3 bulk soil

characteristic: description

characteristic: elevation

characteristic: environmental medium

text: Switzerland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Salgesch, Valais

characteristic: geographic location (region and locality)

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: organism

characteristic: pH

text: soil sieving

text: uncultured eukaryote

text: MiSeq

text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

text: CH-01-FO-SU4 bulk soil

text: Switzerland

text: Crans-Montana, Valais

text: uncultured eukaryote

characteristic: pH method

characteristic: pcr conditions

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text: soil sieving

characteristic: sample material processing

characteristic: sample weight for DNA extraction

text: uncultured eukaryote

text: MiSeq

characteristic: sequencing method

characteristic: slope aspect

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text: in LifeGuard® (Qiagen) at -20degC

characteristic: storage conditions (fresh/frozen/other)

characteristic: target gene

characteristic: target subfragment

characteristic: total organic C method

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characteristic: water content

text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

text: temperate grassland biome [ENVO:01000193]

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: CH-01-OP-SP1 bulk soil

characteristic: description

characteristic: elevation

text: soil [ENVO:00001998]

characteristic: environmental medium

text: Switzerland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Varen, Valais

characteristic: geographic location (region and locality)

text: soil surface layer [ENVO:04000010]

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: organism

characteristic: pH

text: soil sieving

text: uncultured eukaryote

text: MiSeq

text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

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characteristic: INSDC center name

characteristic: INSDC first public

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text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

text: CH-01-OP-SP2 bulk soil

text: soil [ENVO:00001998]

text: Switzerland

text: Salgesch, Valais

text: soil surface layer [ENVO:04000010]

text: uncultured eukaryote

characteristic: pH method

characteristic: pcr conditions

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characteristic: project name

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characteristic: sequencing method

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characteristic: storage conditions (fresh/frozen/other)

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text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

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text: public

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characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: CH-01-OP-SP3 bulk soil

characteristic: description

characteristic: elevation

text: soil [ENVO:00001998]

characteristic: environmental medium

text: Switzerland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Salgesch, Valais

characteristic: geographic location (region and locality)

text: soil surface layer [ENVO:04000010]

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: organism

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text: soil sieving

text: uncultured eukaryote

text: MiSeq

text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

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text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

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text: CH-01-OP-SP4 bulk soil

text: soil [ENVO:00001998]

text: Switzerland

text: Crans-Montana, Valais

text: soil surface layer [ENVO:04000010]

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characteristic: pH method

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text: uncultured eukaryote

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characteristic: sequencing method

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characteristic: storage conditions (fresh/frozen/other)

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text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

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characteristic: INSDC center name

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characteristic: altitude

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: CH-01-OP-SP5 bulk soil

characteristic: description

characteristic: elevation

text: soil [ENVO:00001998]

characteristic: environmental medium

text: Switzerland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Salgesch, Valais

characteristic: geographic location (region and locality)

text: soil surface layer [ENVO:04000010]

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text: MiSeq

text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

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text: public

characteristic: INSDC status

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characteristic: Submitter Id

characteristic: altitude

text: CH-01-OP-SP6 bulk soil

text: soil [ENVO:00001998]

text: Switzerland

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characteristic: pH method

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text: uncultured eukaryote

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characteristic: sequencing method

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characteristic: storage conditions (fresh/frozen/other)

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text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

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characteristic: collection date

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characteristic: depth

text: CH-01-OP-SU1 bulk soil

characteristic: description

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text: soil [ENVO:00001998]

characteristic: environmental medium

text: Switzerland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Varen, Valais

characteristic: geographic location (region and locality)

text: soil surface layer [ENVO:04000010]

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text: MiSeq

text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

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characteristic: INSDC center name

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text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

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text: CH-01-OP-SU2 bulk soil

text: soil [ENVO:00001998]

text: Switzerland

text: Salgesch, Valais

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characteristic: sequencing method

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characteristic: storage conditions (fresh/frozen/other)

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text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

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characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: CH-01-OP-SU3 bulk soil

characteristic: description

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text: soil [ENVO:00001998]

characteristic: environmental medium

text: Switzerland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Salgesch, Valais

characteristic: geographic location (region and locality)

text: soil surface layer [ENVO:04000010]

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: organism

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text: uncultured eukaryote

text: MiSeq

text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

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characteristic: INSDC center name

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text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

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text: CH-01-OP-SU4 bulk soil

text: soil [ENVO:00001998]

text: Switzerland

text: Crans-Montana, Valais

text: soil surface layer [ENVO:04000010]

text: uncultured eukaryote

characteristic: pH method

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characteristic: sequencing method

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characteristic: storage conditions (fresh/frozen/other)

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characteristic: water content method


characteristic: ENA-CHECKLIST

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characteristic: altitude

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characteristic: collection date

characteristic: composite design/sieving (if any)

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text: CH-01-OP-SU5 bulk soil

characteristic: description

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text: soil [ENVO:00001998]

characteristic: environmental medium

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characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Salgesch, Valais

characteristic: geographic location (region and locality)

text: soil surface layer [ENVO:04000010]

characteristic: local environmental context

characteristic: negative control type

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characteristic: organism

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text: MiSeq

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characteristic: title

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characteristic: ENA-CHECKLIST

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text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

text: CH-01-OP-SU6 bulk soil

text: soil [ENVO:00001998]

text: Switzerland

text: Salgesch, Valais

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text: uncultured eukaryote

characteristic: pH method

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text: uncultured eukaryote

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characteristic: sequencing method

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characteristic: storage conditions (fresh/frozen/other)

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text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

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characteristic: INSDC center name

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characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: CH-02-FO-SP1 bulk soil

characteristic: description

characteristic: elevation

characteristic: environmental medium

text: Switzerland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Bex, Vaud

characteristic: geographic location (region and locality)

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: organism

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text: soil sieving

text: uncultured eukaryote

text: MiSeq

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characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

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characteristic: INSDC center name

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characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

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text: CH-02-FO-SP2 bulk soil

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characteristic: sequencing method

characteristic: slope aspect

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characteristic: storage conditions (fresh/frozen/other)

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characteristic: water content

text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

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characteristic: INSDC center name

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characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: CH-02-FO-SP3 bulk soil

characteristic: description

characteristic: elevation

characteristic: environmental medium

text: Switzerland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Bex, Vaud

characteristic: geographic location (region and locality)

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

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text: uncultured eukaryote

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text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

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characteristic: INSDC center name

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characteristic: INSDC status

characteristic: SRA accession

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characteristic: altitude

text: CH-02-FO-SP4 bulk soil

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text: Bex, Vaud

text: uncultured eukaryote

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characteristic: storage conditions (fresh/frozen/other)

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characteristic: water content

text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

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characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

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characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

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text: CH-02-FO-SP5 bulk soil

characteristic: description

characteristic: elevation

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text: Switzerland

characteristic: geographic location (country and/or sea)

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characteristic: geographic location (longitude)

text: Bex, Vaud

characteristic: geographic location (region and locality)

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characteristic: negative control type

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text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

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characteristic: External Id

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characteristic: INSDC center name

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text: public

characteristic: INSDC status

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characteristic: altitude

text: CH-02-FO-SP6 bulk soil

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text: Bex, Vaud

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characteristic: sequencing method

characteristic: slope aspect

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characteristic: storage conditions (fresh/frozen/other)

characteristic: target gene

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characteristic: total organic C method

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characteristic: water content

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characteristic: ENA-CHECKLIST

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characteristic: INSDC center name

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characteristic: broad-scale environmental context

characteristic: collection date

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text: CH-02-FO-SU1 bulk soil

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characteristic: ENA-CHECKLIST

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characteristic: storage conditions (fresh/frozen/other)

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characteristic: ENA-CHECKLIST

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characteristic: collection date

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text: CH-02-FO-SU3 bulk soil

characteristic: description

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characteristic: geographic location (country and/or sea)

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characteristic: ENA-CHECKLIST

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characteristic: storage conditions (fresh/frozen/other)

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text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

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characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: CH-02-FO-SU5 bulk soil

characteristic: description

characteristic: elevation

characteristic: environmental medium

text: Switzerland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Bex, Vaud

characteristic: geographic location (region and locality)

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: organism

characteristic: pH

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text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

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characteristic: INSDC center name

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characteristic: INSDC status

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characteristic: altitude

text: CH-02-OP-SP1 bulk soil

text: soil [ENVO:00001998]

text: Switzerland

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text: soil surface layer [ENVO:04000010]

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characteristic: pH method

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characteristic: sequencing method

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characteristic: storage conditions (fresh/frozen/other)

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text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

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characteristic: Submitter Id

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characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: CH-02-OP-SP2 bulk soil

characteristic: description

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text: soil [ENVO:00001998]

characteristic: environmental medium

text: Switzerland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Gryon, Vaud

characteristic: geographic location (region and locality)

text: soil surface layer [ENVO:04000010]

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characteristic: title

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characteristic: ENA-CHECKLIST

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text: CH-02-OP-SP3 bulk soil

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characteristic: sequencing method

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characteristic: storage conditions (fresh/frozen/other)

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text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

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characteristic: collection date

characteristic: composite design/sieving (if any)

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text: CH-02-OP-SP4 bulk soil

characteristic: description

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text: soil [ENVO:00001998]

characteristic: environmental medium

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characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Bex, Vaud

characteristic: geographic location (region and locality)

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characteristic: title

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characteristic: ENA-CHECKLIST

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text: public

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characteristic: Submitter Id

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text: CH-02-OP-SP5 bulk soil

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characteristic: storage conditions (fresh/frozen/other)

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characteristic: ENA-CHECKLIST

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characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: CH-02-OP-SU1 bulk soil

characteristic: description

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characteristic: environmental medium

text: Switzerland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Bex, Vaud

characteristic: geographic location (region and locality)

text: soil surface layer [ENVO:04000010]

characteristic: local environmental context

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characteristic: title

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characteristic: ENA-CHECKLIST

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characteristic: storage conditions (fresh/frozen/other)

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text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

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characteristic: ENA-LAST-UPDATE

characteristic: External Id

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characteristic: INSDC center name

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characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: CH-02-OP-SU3 bulk soil

characteristic: description

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characteristic: environmental medium

text: Switzerland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

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characteristic: geographic location (region and locality)

text: soil surface layer [ENVO:04000010]

characteristic: local environmental context

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characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

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characteristic: INSDC center name

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text: public

characteristic: INSDC status

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text: CH-02-OP-SU4 bulk soil

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characteristic: sequencing method

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characteristic: storage conditions (fresh/frozen/other)

characteristic: target gene

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text: heating at 105degC for 24 hours

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characteristic: ENA-CHECKLIST

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characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

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text: CH-02-OP-SU5 bulk soil

characteristic: description

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characteristic: environmental medium

text: Switzerland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Bex, Vaud

characteristic: geographic location (region and locality)

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characteristic: pH

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characteristic: title

text: heating at 105degC for 24 hours