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text: Ocean water

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characteristic: GISAID Accession ID

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tag: Namespace:OH-PACT consortium

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text: ERASMUS MC, UNIVERISTY MEDICAL CENTER

characteristic: broker name

text: not provided

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text: OH-PACT consortium

characteristic: collector name

text: Human coronavirus 2019

characteristic: common name

text: A SARS-CoV-2 specfic multiplex PCR for Nanopore sequencing was performed, similar to amplicon-based approaches as previously described. In short, primers for 86 overlapping amplicons spanning the entire genome were designed using primal. The amplicon length was set to 500bp with 75bp overlap between the different amplicons. The libraries were generated using the native barcode kits from Nanopore (EXP-NBD104 and EXP-NBD114 and SQK-LSK109) and sequenced on a R9.4 flow cell multiplexing up to 24 samples per sequence run. Raw data was demultiplexed, amplicon primers were trimmed and human data was removed by mapping against the human reference genome.

text: Poland

text: not provided

characteristic: geographic location (region and locality)

text: mink

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characteristic: host health state

text: Neovison vison

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text: not provided

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text: restricted access

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text: Severe acute respiratory syndrome coronavirus 2

text: active surveillance in response to outbreak

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text: Ocean water

text: Ocean water

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text: Ocean water

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text: Arctic Ocean

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text: tetra-nucleotide frequencies and co-abundance, --minContig 2000 --maxEdges 500

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text: Ocean water

text: Ocean water

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