characteristic: ENA-CHECKLIST

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characteristic: ENA-LAST-UPDATE

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text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

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text: public

characteristic: INSDC status

characteristic: SRA accession

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text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Acetate; Light condition: Dark; Replicate: A; Time: 24h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1405765.8 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 109047.6 cells per ml; epifluorescent microscopy

text: aerobic

text: intital denaturation:98degC_3min;denaturation:98degC_10s; annealing:52degC_30s;elongation:72degC_30s;number of cycles:30; final elongation:72degC_5min

text: pufM_uniF: GGNAAYYTNTWYTAYAAY; pufM_uniR: YCCATNGTCCANCKCCARAACCNTTYCA

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

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text: NMFRI

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text: DADA2 version 1.18.0; function: removeBimeraDenovo; method=pooled

unit: m

text: Treatment: Acetate; Light condition: Dark; Replicate: B; Time: 24h

text: coniferous forest

text: Czech Republic

unit: DD

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1360724.4 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 93638.7 cells per ml; epifluorescent microscopy

characteristic: organism count

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text: less than 6 months

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text: Treatment: Acetate; Light condition: Dark; Replicate: C; Time: 24h

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text: South Bohemia, Lake Cep

text: freshwater lake

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text: total prokaryotes; 1387986.3 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 72303.3 cells per ml; epifluorescent microscopy

text: aerobic

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text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


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text: NMFRI

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text: Treatment: Acetate; Light condition: IR; Replicate: A; Time: 24h

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characteristic: geographic location (longitude)

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text: freshwater metagenome

text: total prokaryotes; 1815879.6 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 182536.2 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

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characteristic: chimera check software

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text: Treatment: Acetate; Light condition: IR; Replicate: B; Time: 24h

characteristic: description

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text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

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characteristic: organism

text: total prokaryotes; 1436583.6 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 132753.6 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


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text: NMFRI

text: public

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text: Treatment: Acetate; Light condition: IR; Replicate: C; Time: 24h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1531407.6 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 136309.5 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

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characteristic: pcr conditions

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text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

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text: less than 6 months

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text: freshwater metagenome

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characteristic: sequencing method

text: pufM

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characteristic: ENA-CHECKLIST

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text: NMFRI

characteristic: INSDC center name

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characteristic: SRA accession

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text: freshwater biome

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characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Control; Light condition: Dark; Replicate: A; Time: 48h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1431842.4 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 73488.6 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


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characteristic: ENA-LAST-UPDATE

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text: NMFRI

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text: Treatment: Control; Light condition: Dark; Replicate: B; Time: 48h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1524295.8 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 92453.4 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

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text: freshwater biome

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characteristic: chimera check software

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characteristic: depth

text: Treatment: Control; Light condition: Dark; Replicate: C; Time: 48h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1431842.4 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 75859.2 cells per ml; epifluorescent microscopy

text: aerobic

text: intital denaturation:98degC_3min;denaturation:98degC_10s; annealing:52degC_30s;elongation:72degC_30s;number of cycles:30; final elongation:72degC_5min

text: pufM_uniF: GGNAAYYTNTWYTAYAAY; pufM_uniR: YCCATNGTCCANCKCCARAACCNTTYCA

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


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text: NMFRI

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text: DADA2 version 1.18.0; function: removeBimeraDenovo; method=pooled

text: Treatment: Control; Light condition: IR; Replicate: A; Time: 48h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

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text: freshwater metagenome

text: total prokaryotes; 1387986.3 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 107862.3 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

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characteristic: pcr conditions

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text: pufM study

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text: Ruttner Water Sampler (model 11.003KC)

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text: less than 6 months

characteristic: sample storage duration

text: Centre Algatech, freezere on the coridor

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

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characteristic: ENA-LAST-UPDATE

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text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

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text: public

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text: freshwater biome

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characteristic: chimera check software

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characteristic: depth

text: Treatment: Control; Light condition: IR; Replicate: B; Time: 48h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1634528.7 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 93638.7 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


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characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Control; Light condition: IR; Replicate: C; Time: 48h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1411692.3 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 75859.2 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

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text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

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characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

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characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Dilution; Light condition: Dark; Replicate: A; Time: 48h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1563410.7 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 119715.3 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


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characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Dilution; Light condition: Dark; Replicate: B; Time: 48h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1500589.8 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 99565.2 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

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characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

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characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Dilution; Light condition: Dark; Replicate: C; Time: 48h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1610822.7 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 107862.3 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Dilution; Light condition: IR; Replicate: A; Time: 48h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1615563.9 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 75859.2 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

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characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


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characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Dilution; Light condition: IR; Replicate: B; Time: 48h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1473327.9 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 94824 cells per ml; epifluorescent microscopy

text: aerobic

text: intital denaturation:98degC_3min;denaturation:98degC_10s; annealing:52degC_30s;elongation:72degC_30s;number of cycles:30; final elongation:72degC_5min

text: pufM_uniF: GGNAAYYTNTWYTAYAAY; pufM_uniR: YCCATNGTCCANCKCCARAACCNTTYCA

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: DADA2 version 1.18.0; function: removeBimeraDenovo; method=pooled

text: Treatment: Dilution; Light condition: IR; Replicate: C; Time: 48h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

text: Centre Algatech, freezere on the coridor

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Acetate; Light condition: Dark; Replicate: A; Time: 48h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1539704.7 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 143421.3 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Acetate; Light condition: Dark; Replicate: B; Time: 48h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1236267.9 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 104306.4 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Acetate; Light condition: Dark; Replicate: C; Time: 48h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1384430.4 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 124456.5 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Acetate; Light condition: IR; Replicate: A; Time: 48h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1263529.8 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 110232.9 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Acetate; Light condition: IR; Replicate: B; Time: 48h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1421174.7 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 117344.7 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Acetate; Light condition: IR; Replicate: C; Time: 48h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1134332.1 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 74673.9 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Control; Light condition: Dark; Replicate: A; Time: 72h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1549187.1 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 151718.4 cells per ml; epifluorescent microscopy

text: aerobic

text: intital denaturation:98degC_3min;denaturation:98degC_10s; annealing:52degC_30s;elongation:72degC_30s;number of cycles:30; final elongation:72degC_5min

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: DADA2 version 1.18.0; function: removeBimeraDenovo; method=pooled

text: Treatment: Control; Light condition: Dark; Replicate: B; Time: 72h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1300274.1 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 138680.1 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

text: pufM_uniF: GGNAAYYTNTWYTAYAAY; pufM_uniR: YCCATNGTCCANCKCCARAACCNTTYCA

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Control; Light condition: Dark; Replicate: C; Time: 72h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1527851.7 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 96009.3 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: Centre Algatech, freezere on the coridor

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Control; Light condition: IR; Replicate: A; Time: 72h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1313312.4 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 118530 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Control; Light condition: IR; Replicate: B; Time: 72h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1534963.5 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 112603.5 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Control; Light condition: IR; Replicate: C; Time: 72h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1641640.5 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 158830.2 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Dilution; Light condition: Dark; Replicate: A; Time: 72h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1392727.5 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 207427.5 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Dilution; Light condition: Dark; Replicate: B; Time: 72h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1626231.6 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 182536.2 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

text: DADA2 version 1.18.0; function: removeBimeraDenovo; method=pooled

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Dilution; Light condition: Dark; Replicate: C; Time: 72h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1466216.1 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 200315.7 cells per ml; epifluorescent microscopy

text: aerobic

text: intital denaturation:98degC_3min;denaturation:98degC_10s; annealing:52degC_30s;elongation:72degC_30s;number of cycles:30; final elongation:72degC_5min

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Dilution; Light condition: IR; Replicate: A; Time: 72h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1659420 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 228762.9 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

text: pufM_uniF: GGNAAYYTNTWYTAYAAY; pufM_uniR: YCCATNGTCCANCKCCARAACCNTTYCA

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Dilution; Light condition: IR; Replicate: B; Time: 72h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1612008 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 167127.3 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: Centre Algatech, freezere on the coridor

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Dilution; Light condition: IR; Replicate: C; Time: 72h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1779135.3 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 237060 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Acetate; Light condition: Dark; Replicate: A; Time: 72h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1455548.4 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 67562.1 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Acetate; Light condition: Dark; Replicate: B; Time: 72h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1337018.4 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 58079.7 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Acetate; Light condition: Dark; Replicate: C; Time: 72h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1795729.5 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 109047.6 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: DADA2 version 1.18.0; function: removeBimeraDenovo; method=pooled

text: Treatment: Acetate; Light condition: IR; Replicate: A; Time: 72h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1553928.3 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 86526.9 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Acetate; Light condition: IR; Replicate: B; Time: 72h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1395098.1 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 64006.2 cells per ml; epifluorescent microscopy

text: aerobic

text: intital denaturation:98degC_3min;denaturation:98degC_10s; annealing:52degC_30s;elongation:72degC_30s;number of cycles:30; final elongation:72degC_5min

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Acetate; Light condition: IR; Replicate: C; Time: 72h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1230341.4 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 67562.1 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

text: pufM_uniF: GGNAAYYTNTWYTAYAAY; pufM_uniR: YCCATNGTCCANCKCCARAACCNTTYCA

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Control; Light condition: Dark; Replicate: A; Time: 96h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1446066 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 66376.8 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: Centre Algatech, freezere on the coridor

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Control; Light condition: Dark; Replicate: B; Time: 96h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1307385.9 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 62820.9 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Control; Light condition: Dark; Replicate: C; Time: 96h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1289606.4 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 66376.8 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Control; Light condition: IR; Replicate: A; Time: 96h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1276568.1 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 42670.8 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

text: DADA2 version 1.18.0; function: removeBimeraDenovo; method=pooled

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Control; Light condition: IR; Replicate: B; Time: 96h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1690237.8 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 56894.4 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Control; Light condition: IR; Replicate: C; Time: 96h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1792173.6 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 55709.1 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Dilution; Light condition: Dark; Replicate: A; Time: 96h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 2012639.4 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 348478.2 cells per ml; epifluorescent microscopy

text: aerobic

text: intital denaturation:98degC_3min;denaturation:98degC_10s; annealing:52degC_30s;elongation:72degC_30s;number of cycles:30; final elongation:72degC_5min

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Dilution; Light condition: Dark; Replicate: B; Time: 96h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1810875 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 251283.6 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

text: pufM_uniF: GGNAAYYTNTWYTAYAAY; pufM_uniR: YCCATNGTCCANCKCCARAACCNTTYCA

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Dilution; Light condition: Dark; Replicate: C; Time: 96h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1997230.5 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 328328.1 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: Centre Algatech, freezere on the coridor

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Dilution; Light condition: IR; Replicate: A; Time: 96h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 2126428.2 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 382851.9 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Dilution; Light condition: IR; Replicate: B; Time: 96h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 2367044.1 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 355590 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: DADA2 version 1.18.0; function: removeBimeraDenovo; method=pooled

text: Treatment: Dilution; Light condition: IR; Replicate: C; Time: 96h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 2092054.5 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 356775.3 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Acetate; Light condition: Dark; Replicate: A; Time: 96h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 2313705.6 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 138680.1 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Acetate; Light condition: Dark; Replicate: B; Time: 96h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 2644404.3 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 101935.8 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Acetate; Light condition: Dark; Replicate: C; Time: 96h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1923741.9 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 97194.6 cells per ml; epifluorescent microscopy

text: aerobic

text: intital denaturation:98degC_3min;denaturation:98degC_10s; annealing:52degC_30s;elongation:72degC_30s;number of cycles:30; final elongation:72degC_5min

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Acetate; Light condition: IR; Replicate: A; Time: 96h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1209006 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 55709.1 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

text: pufM_uniF: GGNAAYYTNTWYTAYAAY; pufM_uniR: YCCATNGTCCANCKCCARAACCNTTYCA

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Acetate; Light condition: IR; Replicate: B; Time: 96h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1472142.6 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 81785.7 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: Centre Algatech, freezere on the coridor

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Acetate; Light condition: IR; Replicate: C; Time: 96h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1219673.7 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 36744.3 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


T brucei TTseq

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: T brucei TTseq

tag: Namespace:Department of Veterinary Sciences, LMU Muenchen

characteristic: Submitter Id

characteristic: collection date

text: Trypanosoma brucei brucei

characteristic: description

text: Uganda

characteristic: geographic location (country and/or sea)

text: Trypanosoma brucei brucei

characteristic: organism

text: Trypanosoma brucei brucei

text: BSF 427 TTseq

characteristic: title


T brucei SLAMseq RNA Halflives

text: Department of Veterinary Sciences, LMU Muenchen

text: public

text: T brucei SLAMseq RNA Halflives

text: Trypanosoma brucei brucei

text: Uganda

text: Trypanosoma brucei brucei

text: Trypanosoma brucei brucei

text: BSF 427 SLAMseq RNA Halflives


T brucei SLAMseq Labeling efficiency 1

text: public

text: T brucei SLAMseq Labeling efficiency 1

text: Trypanosoma brucei brucei

text: Uganda

text: Trypanosoma brucei brucei

text: Trypanosoma brucei brucei

text: BSF 427 SLAMseq Labeling efficiency 1


T brucei SLAMseq Labeling efficiency 2

text: public

text: T brucei SLAMseq Labeling efficiency 2

text: Trypanosoma brucei brucei

text: Uganda

text: Trypanosoma brucei brucei

text: Trypanosoma brucei brucei

text: BSF 427 SLAMseq Labeling efficiency 2


T brucei RNAseq

text: public

text: T brucei RNAseq

text: Trypanosoma brucei brucei

text: Uganda

text: Trypanosoma brucei brucei

text: Trypanosoma brucei brucei

text: BSF 427 SLAMseq RNAseq


characteristic: ENA first public

text: Hebrew University, Genetics Department

text: public

tag: Namespace:Hebrew University, Genetics Department

text: ArrayExpress

characteristic: broker name

text: Diploid differentiated ESCs

text: not collected

text: human

characteristic: common name

text: Protocols: Cells were washed with PBS and trypsinized Cells were encapsulated with hydrogel beads, droplet oil and RT/lysis mixture. The encapsulated cells were then exposed to UV light, releasing photocleavable primers from hydrogel beads. Subsequently, reverse transcription was performed and the beads filtered out. The RT product was digested using ExoI and HinFI and purified using AMpure XP beads. Then, the second strand cDNA synthesis and in vitro transcription steps were carried out. Next, the resulting RNA molecules were fragmented and a second reverse transcription step was carried out using random hexamers. The cDNA was then purified using Ampure XP beads and quantified via qPCR.

text: Neuronal differentiation

characteristic: developmental stage

text: Diploid

characteristic: genotype

text: not collected

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Diploid


characteristic: ENA-CHECKLIST

characteristic: External Id

text: Hebrew University, Genetics Department

characteristic: INSDC center name

text: public

text: ArrayExpress

text: Haploid differentiated ESCs

text: not collected

text: human

text: Neuronal differentiation

text: Haploid

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: NMFRI

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: pure culture of Dinoroseobacter shibae

text: Czech Republic

text: Dinoroseobacter shibae

text: Dinoroseobacter shibae

text: Dinoroseobacter shibae


text: uzh

text: public

characteristic: SRA accession

text: ena-SAMPLE-TAB-13-12-2023-13:22:36:394-146595

characteristic: Submitter Id

text: marine

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

text: marine eDNA

characteristic: description

text: water

characteristic: environmental medium

text: CND

characteristic: experimental factor

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Shark Bay

characteristic: local environmental context

text: fish metagenome

characteristic: project name

text: fish metagenome


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: uzh

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