text: public

tag: Namespace:1.Graduate Institute of Pathology, National Taiwan University, Taipei, Taiwan 2.School of Medicine, National Taiwan University, Taipei, Taiwan

text: ArrayExpress

characteristic: broker name

text: human

text: Protocols: All human tissue samples were obtained from the archive of Department of Pathology, National Taiwan University Hospital (NTUH). Hybridization cocktail with 25μl RNA probe mixture were made, containing 200μl buffer R (NanoString Technologies, Seattle, WA, USA) and 25μl DEPC water for each slide. We then applied the RNA probe mixture to each sample slide. Hybrislip (Thermo Fisher Scientific, Waltham, MA, USA) was used to cover to the sample slides. Then we incubated the slides in HyBEZ (ACDBio), (Advanced Cell Diagnostics, Inc., Newark, CA, USA) in a humid chamber at 37℃ for 16-24 h. On the next day, we washed the uncovered slides with prewarmed Stringent wash solution and SSC Solution (Sigma Aldrich, Inc. St. Louis, Missouri, USA). All the off-target probes were removed away from the slides. Then, we blocked the non-specific binding with 200μl Buffer W (Nanostring Technologies, Seattle, WA, USA) for 30 minutes in the humidity DPEC-wetted chamber. After blocking, 220 µL of morphology marker mix was applied to each sample and incubated for 1 hr. The tumours were visualized with staining using GeoMx Solid Tumor TME Morphology Kit (NanoString Technologies, Seattle, WA, USA) including 1:40x anti-CD45 (Yellow) plus PanCK (Green), and 1:10X SYTO 13 (nuclei, Blue) . Following the GeoMx DSP Instrument Quick Start Guide (MAN-10133-03), we prepared the PCR products with 2μl GeoMx Seq Code PCR Master Mix and 4 μl GeoMx DSP samples. CTA probe (Cat: GMX-RNA-NGS-CTA-4) was used as positive control and DEPC-water as negative control in this experiment. The following thermocycler programwas used: 37℃ incubation for 30 min, deactivation at 50℃ for 10 min, initial denaturation at 95℃ for 3 min cycles of denaturation, annealing and extension at 95℃, 65℃, and 68℃ for 15 sec, 60 sec, and 30 sec, respectively for cycles, and final extension for the PCR product for 5 min. PCR products were then purified with two rounds of AMPure XP beads (Beckman Coulter, CA, USA) at 1.2X beads-to sample ratio. Libraries were paired-end sequenced on a NextSeq550. Up to 400 M total aligned reads were obtained. NextSeq550-derived FASTQ files for each sample were compiled for each compartment using the fastq program of Illumina, and then demultiplexed and converted to digital count conversion (DCC) by the GeoMx NGS Pipeline. The quality of the RNA library and NGS analysis were performed by the Medical Microbiota Center of the First Core Laboratory, National Taiwan University College of Medicine, Taipei, Taiwan. Additional files contain the H and E staining as well as the sample sequence codes.

text: adult

characteristic: developmental stage

text: polyp of colon

characteristic: disease

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: colorectum

characteristic: organism part

text: Homo sapiens


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: 1.Graduate Institute of Pathology, National Taiwan University, Taipei, Taiwan 2.School of Medicine, National Taiwan University, Taipei, Taiwan

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: human

characteristic: common name

characteristic: description

text: adult

text: polyp of colon

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: colorectum

text: Homo sapiens

characteristic: title


text: public

text: ArrayExpress

characteristic: broker name

text: human

text: adult

characteristic: developmental stage

text: sessile serrated polyp

characteristic: disease

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: colorectum

text: Homo sapiens


characteristic: ENA-CHECKLIST

text: public

text: ArrayExpress

text: human

text: adult

text: Rectal Traditional Serrated Adenoma

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: colorectum

characteristic: organism part

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

tag: Namespace:Leiden University, Leiden Academic Centre for Drug Research (LACDR), department of Drug Development and Safety

text: ArrayExpress

text: hepatocyte

text: human

text: Protocols: Following 8, 24, 48 or 72 hour exposure, cell cultures were washed once with DPBS without calcium and magnesium chloride (Sigma-Aldrich, #D8537). Thereafter, cells were lysed with 50 µL lysis buffer (1:1 DPBS and 2x TempO‐Seq lysis buffer, BioSpyder, mixed ex tempore) for 15 minutes at room temperature and stored at ‐80°C, sealed with an aluminium silver seal (Greiner Bio-One, #676090). PHH (10-donor, LIVERPOOL Cryoplateable Hepatocytes; BioIVT, #X008001-P) were thawed in 25 mL Sekisui XenoTech OptiThaw Hepatocyte Kit (Tebu-bio, #K8000) and centrifuged at 100x g for 10 minutes. Pellet was resuspended in INVITROGRO CP medium (BioIVT, #Z99029) supplemented with TORPEDO antibiotics mix (BioIVT, #Z990007) and seeded at a density of 70,000 cells per well in BioCoat Collagen-I-coated 96-wells plates (Corning, #354407). After 6 hours, PHH were washed with Dulbecco’s phosphate-buffered saline (DPBS) to remove dead cells. PHH were overlaid with 100 µl 0.25 mg/mL cooled Matrigel™ (BD Biosciences, #354230) in INVITROGRO HI medium (BioIVT, #Z99009) supplemented with TORPEDO antibiotics mix (BioIVT, #Z990007) to create a collagen-Matrigel™ sandwich. Cryopreserved differentiated HepaRG® cells (Biopredic International, #HPR116-TA08) were thawed and seeded (day one) according to the manufacturer’s protocol. In short, 72,000 viable cells per well were seeded in collagen-coated (Corning #354236, 0.1 mg/ml in 0.02N acetic acid) 96-well plates (Falcon® #353072) in 100 µL basal hepatic cell medium (Biopredic International, #32551) supplemented with HepaRG® Thawing/Plating/General Purpose Medium (Biopredic International, #ADD670). On the second day of culture, the medium was changed to 100 µL basal hepatic cell medium with HepaRG® Maintenance/Metabolism Medium (Biopredic International, #ADD620) and subsequently renewed on days four and seven. PHH were exposed to the compounds in 100 µL INVITROGRO HI medium (BioIVT, #Z99009) supplemented with TORPEDO antibiotics mix (BioIVT, #Z990007) 24 hours after plating. On day eight in culture, HepaRG® cells were exposed to the compounds in 100 µL basal hepatic cell medium with serum-free HepaRG® Induction Medium (Biopredic International, #ADD650). Cell lysates were analysed at BioClavis (Glasgow, UK) using TempO-Seq targeted RNA sequencing technology with the human whole-transcriptome probeset version 2.0 according to their standard protocol. In TempO-Seq, each Detector Oligo consists of a sequence complementary to an mRNA target plus a universal (i.e. same for every targeted gene) primer binding site. They anneal in immediate juxtaposition to each other on the targeted RNA template such that they can be ligated together. Ligated detector oligos are PCR-amplified using a primer set (single-plex PCR reaction, with a single primer pair for each sample) that introduces both the adaptors required for sequencing and a sample-specific barcode. The barcode sequences flank the target sequence and are inserted appropriately into the standard Illumina adaptors to permit standard dual-index sequencing of the barcodes and deconvolution of sample-specific reads from the sequencing data using the standard Illumina software. All the PCR-amplified and barcoded samples are pooled into a single library for sequencing. Sequencing reads are demultiplexed using the standard sequencing instrument software for each sample using the barcodes to give a FASTQ file for each. Cell lysates were analysed at BioClavis (Glasgow, UK) using TempO-Seq targeted RNA sequencing technology with the human whole-transcriptome probeset version 2.0 according to their standard protocol. In TempO-Seq, each Detector Oligo consists of a sequence complementary to an mRNA target plus a universal (i.e. same for every targeted gene) primer binding site. They anneal in immediate juxtaposition to each other on the targeted RNA template such that they can be ligated together. Ligated detector oligos are PCR-amplified using a primer set (single-plex PCR reaction, with a single primer pair for each sample) that introduces both the adaptors required for sequencing and a sample-specific barcode. The barcode sequences flank the target sequence and are inserted appropriately into the standard Illumina adaptors to permit standard dual-index sequencing of the barcodes and deconvolution of sample-specific reads from the sequencing data using the standard Illumina software. All the PCR-amplified and barcoded samples are pooled into a single library for sequencing. Sequencing reads are demultiplexed using the standard sequencing instrument software for each sample using the barcodes to give a FASTQ file for each.

text: adult

text: normal

text: wild type genotype

characteristic: genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens


text: Leiden University, Leiden Academic Centre for Drug Research (LACDR), department of Drug Development and Safety

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: hepatocyte

text: human

text: adult

text: normal

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens


text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: HepaRG

text: hepatocyte

text: human

characteristic: common name

characteristic: description

text: adult

text: hepatocellular carcinoma

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens


text: public

text: ArrayExpress

characteristic: broker name

characteristic: developmental stage

text: not applicable

text: synthetic construct

characteristic: organism

text: synthetic construct

text: synthetic construct

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Rueda-M,N

text: public

text: Ecuador

characteristic: geographic location (country and/or sea)

text: Heliconius sara

text: Heliconius sara

text: Male

characteristic: sex


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

tag: Namespace:Department of Biology, University of Florence

text: organism substance

characteristic: broad-scale environmental context

characteristic: collection date

text: AOM model rat control diet T0

text: feaces

characteristic: environmental medium

text: France

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Rattus norvegicus

characteristic: host scientific name

text: excreta

characteristic: local environmental context

text: rat metagenome

characteristic: project name

text: rat metagenome


text: Department of Biology, University of Florence

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: organism substance

text: AOM model rat control diet T3

characteristic: description

text: feaces

text: France

text: Rattus norvegicus

text: excreta

text: rat metagenome

characteristic: organism

text: rat metagenome


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characteristic: ENA-LAST-UPDATE

characteristic: External Id

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text: organism substance

text: AOM model rat control diet T0

text: feaces

text: France

characteristic: geographic location (country and/or sea)

text: Rattus norvegicus

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text: rat metagenome

text: rat metagenome

characteristic: title


text: public

text: organism substance

characteristic: broad-scale environmental context

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text: AOM model rat meat based diet T0

text: feaces

characteristic: environmental medium

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Rattus norvegicus

characteristic: host scientific name

text: excreta

text: rat metagenome

text: rat metagenome


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text: organism substance

text: AOM model rat meat based diet T3

text: feaces

text: France

text: Rattus norvegicus

text: excreta

characteristic: local environmental context

text: rat metagenome

characteristic: project name

text: rat metagenome


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

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characteristic: SRA accession

characteristic: Submitter Id

text: organism substance

text: AOM model rat meat+tocopherol based diet T0

characteristic: description

text: feaces

text: France

text: Rattus norvegicus

text: excreta

text: rat metagenome

characteristic: organism

text: rat metagenome


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characteristic: External Id

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text: organism substance

characteristic: broad-scale environmental context

characteristic: collection date

text: AOM model rat meat+tocopherol based diet T3

text: feaces

text: France

characteristic: geographic location (country and/or sea)

text: Rattus norvegicus

text: excreta

text: rat metagenome

text: rat metagenome

characteristic: title


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: organism substance

text: AOM model rat pesco-vegetarian based diet T0

text: feaces

characteristic: environmental medium

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Rattus norvegicus

characteristic: host scientific name

text: excreta

text: rat metagenome

text: rat metagenome


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: organism substance

text: AOM model rat pesco-vegetarian based diet T3

text: feaces

text: France

text: Rattus norvegicus

text: excreta

characteristic: local environmental context

text: rat metagenome

characteristic: project name

text: rat metagenome


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: organism substance

text: PIRC model rat pesco-vegetarian based diet T0

characteristic: description

text: feaces

text: Italy

text: Rattus norvegicus

text: excreta

text: rat metagenome

characteristic: organism

text: rat metagenome


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: organism substance

characteristic: broad-scale environmental context

characteristic: collection date

text: PIRC model rat pesco-vegetarian based diet T1

text: feaces

text: Italy

characteristic: geographic location (country and/or sea)

text: Rattus norvegicus

text: excreta

text: rat metagenome

text: rat metagenome

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: organism substance

text: PIRC model rat control based diet T0

text: feaces

characteristic: environmental medium

text: Italy

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Rattus norvegicus

characteristic: host scientific name

text: excreta

text: rat metagenome

text: rat metagenome


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: organism substance

text: PIRC model rat control based diet T1

text: feaces

text: Italy

text: Rattus norvegicus

text: excreta

characteristic: local environmental context

text: rat metagenome

characteristic: project name

text: rat metagenome


characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: organism substance

text: PIRC model rat meat based diet T0

characteristic: description

text: feaces

text: Italy

text: Rattus norvegicus

text: excreta

text: rat metagenome

characteristic: organism

text: rat metagenome


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: organism substance

characteristic: broad-scale environmental context

characteristic: collection date

text: PIRC model rat meat based diet T1

text: feaces

text: Italy

characteristic: geographic location (country and/or sea)

text: Rattus norvegicus

text: excreta

text: rat metagenome

text: rat metagenome

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: organism substance

text: PIRC model rat meat+tocopherol based diet T0

text: feaces

characteristic: environmental medium

text: Italy

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Rattus norvegicus

characteristic: host scientific name

text: excreta

text: rat metagenome

text: rat metagenome


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: organism substance

text: PIRC model rat meat+tocopherol based diet T1

text: feaces

text: Italy

text: Rattus norvegicus

text: excreta

characteristic: local environmental context

text: rat metagenome

characteristic: project name

text: rat metagenome


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: organism substance

text: FMT(AOM) model rat meat based diet T0

characteristic: description

text: feaces

text: France

text: Rattus norvegicus

text: excreta

text: rat metagenome

characteristic: organism

text: rat metagenome


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: organism substance

characteristic: broad-scale environmental context

characteristic: collection date

text: FMT(AOM) model rat meat based diet T14

text: feaces

text: France

characteristic: geographic location (country and/or sea)

text: Rattus norvegicus

text: excreta

text: rat metagenome

text: rat metagenome

characteristic: title


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: organism substance

text: FMT(AOM) model rat meat based diet T2

text: feaces

characteristic: environmental medium

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Rattus norvegicus

characteristic: host scientific name

text: excreta

text: rat metagenome

text: rat metagenome


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: organism substance

text: FMT(AOM) model rat meat+tocopherol based diet T0

text: feaces

text: France

text: Rattus norvegicus

text: excreta

characteristic: local environmental context

text: rat metagenome

characteristic: project name

text: rat metagenome


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: organism substance

text: FMT(AOM) model rat meat+tocopherol based diet T14

characteristic: description

text: feaces

text: France

text: Rattus norvegicus

text: excreta

text: rat metagenome

characteristic: organism

text: rat metagenome


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: organism substance

characteristic: broad-scale environmental context

characteristic: collection date

text: FMT(AOM) model rat meat+tocopherol based diet T2

text: feaces

text: France

characteristic: geographic location (country and/or sea)

text: Rattus norvegicus

text: excreta

text: rat metagenome

text: rat metagenome

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: organism substance

text: faecal inoculum from PIRC model rat control based diet

text: feaces

characteristic: environmental medium

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Rattus norvegicus

characteristic: host scientific name

text: excreta

text: rat metagenome

text: rat metagenome


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: organism substance

text: faecal inoculum from PIRC model rat pesco-vegetarian based diet

text: feaces

text: France

text: Rattus norvegicus

text: excreta

characteristic: local environmental context

text: rat metagenome

characteristic: project name

text: rat metagenome


characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: organism substance

text: faecal inoculum from PIRC model rat meat based diet

characteristic: description

text: feaces

text: France

text: Rattus norvegicus

text: excreta

text: rat metagenome

characteristic: organism

text: rat metagenome


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: organism substance

characteristic: broad-scale environmental context

characteristic: collection date

text: faecal inoculum from PIRC model rat meat+tocopherol based diet

text: feaces

text: France

characteristic: geographic location (country and/or sea)

text: Rattus norvegicus

text: excreta

text: rat metagenome

text: rat metagenome

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: organism substance

text: FMT(AOM) model rat pesco-vegetarian based diet T0

text: feaces

characteristic: environmental medium

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Rattus norvegicus

characteristic: host scientific name

text: excreta

text: rat metagenome

text: rat metagenome


text: Department of Biology, University of Florence

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: organism substance

text: FMT(AOM) model rat pesco-vegetarian based diet T14

text: feaces

text: France

text: Rattus norvegicus

text: excreta

characteristic: local environmental context

text: rat metagenome

characteristic: project name

text: rat metagenome


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: organism substance

text: FMT(AOM) model rat pesco-vegetarian based diet T2

characteristic: description

text: feaces

text: France

text: Rattus norvegicus

text: excreta

text: rat metagenome

characteristic: organism

text: rat metagenome


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: organism substance

characteristic: broad-scale environmental context

characteristic: collection date

text: FMT(AOM) model rat control based diet T0

text: feaces

text: France

characteristic: geographic location (country and/or sea)

text: Rattus norvegicus

text: excreta

text: rat metagenome

text: rat metagenome

characteristic: title


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: organism substance

text: FMT(AOM) model rat control based diet T14

text: feaces

characteristic: environmental medium

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Rattus norvegicus

characteristic: host scientific name

text: excreta

text: rat metagenome

text: rat metagenome


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: organism substance

text: FMT(AOM) model rat control based diet T2

text: feaces

text: France

text: Rattus norvegicus

text: excreta

characteristic: local environmental context

text: rat metagenome

characteristic: project name

text: rat metagenome


E-MTAB-12686:DT-DAOY-VIN N = 1

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NEWCASTLE UNIVERSITY

text: public

characteristic: SRA accession

text: E-MTAB-12686:DT-DAOY-VIN N = 1

tag: Namespace:NEWCASTLE UNIVERSITY

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: Daoy

text: SHH medulloblastoma

characteristic: clinical information

text: human

characteristic: common name

text: Protocols: Drug-tolerant cell lines were pelleted and snap frozen in liquid nitrogen. Samples were stored at -80oC until downstream use. RNA was isolated utilising the NucleoSpin® RNA Plus kit (Machery-Nagel) as per the manufacturer’s instructions, with an elution volume of 40 μL. RNA concentration and purity was analysed using a Nanodrop2000 spectrophotometer (Thermo Scientific) and only samples which met the following criteria were submitted for library preparation: RNA concentration > 100 ng/μL; 260/280 ratio 1.9 – 2.1; 260/230 ratio 1.8 – 2.2. Further quality control was undertaken using an Agilent TapeStation 4200 with the Agilent RNA ScreenTape Assay Kit (Agilent; 5067-5576 and 5067-5577) to test RNA integrity levels and a Qubit Fluorometer with the Qubit RNA BR Assay Kit (ThermoFisher Scientific; Q10211) to assess RNA quantity. 3’mRNA sequencing (3’mRNA-Seq) libraries were prepared using the QuantSeq 3’ mRNA-Seq library prep kit for Illumina in the forward read direction supplemented with unique molecular index (UMI) as per manufacturer’s instructions (Lexogen; 015.96). The aforementioned kit uses total RNA as input, hence no prior poly(A) enrichment or rRNA depletion is needed. For each sample, cDNA was generated from 250 ng total RNA. First, total RNA was reverse transcribed using oligo-dT primers containing an Illumina-specific linker sequence. First-strand synthesis and RNA removal was followed by random-primed synthesis of the complementary strand. The random primer contained another Illumina-specific linker sequence. The resulting double-stranded cDNA was purified with magnetic beads. Library PCR amplification then introduced the complete adapter sequences required for cluster generation and UMIs which allow elimination of possible PCR duplicates in sequencing datasets (i7 6nt Index Set; 015.96; Lexogen). 14 PCR cycles were found to be optimal for library amplification. Libraries were next quantified using the Qubit Fluorometer and the Qubit dsDNA HS Kit (ThermoFisher Scientific; Q32854), and library fragment-length distributions analysed using the Agilent TapeStation 4200 and the Agilent High Sensitivity D1000 ScreenTape Assay (Agilent; 5067-5584 and 5067-5585). Finally, libraries were pooled in equimolar amounts and final library quantification performed using the KAPA Library Quantification Kit for Illumina (Roche; KK4824).

characteristic: description

text: juvenile stage

characteristic: developmental stage

text: desmoplastic medulloblastoma

characteristic: disease

text: not applicable

characteristic: isolate

characteristic: metastatic site

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: brain

characteristic: organism part

text: Homo sapiens

text: DT-DAOY-VIN N = 1


E-MTAB-12686:DT-DAOY-VIN N = 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: NEWCASTLE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12686:DT-DAOY-VIN N = 2

text: ArrayExpress

text: Daoy

text: SHH medulloblastoma

text: human

text: juvenile stage

text: desmoplastic medulloblastoma

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: brain

text: Homo sapiens

text: DT-DAOY-VIN N = 2

characteristic: title


E-MTAB-12686:DT-DAOY-VIN N = 3

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NEWCASTLE UNIVERSITY

text: public

characteristic: SRA accession

text: E-MTAB-12686:DT-DAOY-VIN N = 3

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: Daoy

text: SHH medulloblastoma

text: human

text: juvenile stage

text: desmoplastic medulloblastoma

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: brain

text: Homo sapiens

text: DT-DAOY-VIN N = 3


E-MTAB-12686:DT-D283-CIS N = 1

text: NEWCASTLE UNIVERSITY

text: public

text: E-MTAB-12686:DT-D283-CIS N = 1

text: ArrayExpress

text: D283 Med

text: group 3 medulloblastoma

characteristic: clinical information

text: human

characteristic: common name

characteristic: description

text: juvenile stage

characteristic: developmental stage

text: medulloblastoma

characteristic: disease

text: not applicable

text: peritoneum

text: Homo sapiens

text: Homo sapiens

text: brain

text: Homo sapiens

text: DT-D283-CIS N = 1


E-MTAB-12686:DT-D283-CIS N = 2

characteristic: ENA-CHECKLIST

text: NEWCASTLE UNIVERSITY

text: public

text: E-MTAB-12686:DT-D283-CIS N = 2

text: ArrayExpress

text: D283 Med

text: group 3 medulloblastoma

text: human

text: juvenile stage

text: medulloblastoma

text: not applicable

characteristic: isolate

text: peritoneum

characteristic: metastatic site

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: brain

characteristic: organism part

text: Homo sapiens

text: DT-D283-CIS N = 2


E-MTAB-12686:DT-D283-CIS N = 3

characteristic: ENA-FIRST-PUBLIC

text: NEWCASTLE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12686:DT-D283-CIS N = 3

text: ArrayExpress

text: D283 Med

text: group 3 medulloblastoma

text: human

text: juvenile stage

text: medulloblastoma

text: not applicable

text: peritoneum

text: Homo sapiens

text: Homo sapiens

text: brain

text: Homo sapiens

text: DT-D283-CIS N = 3


E-MTAB-12686:DT-D458-CIS N = 1

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NEWCASTLE UNIVERSITY

text: public

characteristic: SRA accession

text: E-MTAB-12686:DT-D458-CIS N = 1

characteristic: Submitter Id

text: ArrayExpress

text: group 3 medulloblastoma

text: human

text: juvenile stage

text: medulloblastoma

text: not applicable

text: cerebrospinal fluid

text: Homo sapiens

text: Homo sapiens

text: brain

text: Homo sapiens

text: DT-D458-CIS N = 1

characteristic: title


E-MTAB-12686:DT-D458-CIS N = 2

text: NEWCASTLE UNIVERSITY

text: public

text: E-MTAB-12686:DT-D458-CIS N = 2

text: ArrayExpress

characteristic: broker name

text: group 3 medulloblastoma

characteristic: clinical information

text: human

characteristic: common name

characteristic: description

text: juvenile stage

characteristic: developmental stage

text: medulloblastoma

text: not applicable

text: cerebrospinal fluid

text: Homo sapiens

text: Homo sapiens

text: brain

text: Homo sapiens

text: DT-D458-CIS N = 2


E-MTAB-12686:DT-D458-CIS N = 3

characteristic: ENA-CHECKLIST

text: NEWCASTLE UNIVERSITY

text: public

text: E-MTAB-12686:DT-D458-CIS N = 3

text: ArrayExpress

text: group 3 medulloblastoma

text: human

text: juvenile stage

text: medulloblastoma

characteristic: disease

text: not applicable

characteristic: isolate

text: cerebrospinal fluid

characteristic: metastatic site

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: brain

text: Homo sapiens

text: DT-D458-CIS N = 3


E-MTAB-12686:DT-HDMB-03-CIS N = 1

characteristic: ENA-FIRST-PUBLIC

text: NEWCASTLE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-12686:DT-HDMB-03-CIS N = 1

text: ArrayExpress

text: group 3 medulloblastoma

text: human

text: juvenile stage

text: medulloblastoma

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: brain

characteristic: organism part

text: Homo sapiens

text: DT-HDMB-03-CIS N = 1


E-MTAB-12686:DT-HDMB-03-CIS N = 2

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NEWCASTLE UNIVERSITY

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12686:DT-HDMB-03-CIS N = 2

characteristic: Submitter Id

text: ArrayExpress

text: group 3 medulloblastoma

text: human

text: juvenile stage

text: medulloblastoma

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: brain

text: Homo sapiens

text: DT-HDMB-03-CIS N = 2


E-MTAB-12686:DT-HDMB-03-CIS N = 3

text: NEWCASTLE UNIVERSITY

text: public

text: E-MTAB-12686:DT-HDMB-03-CIS N = 3

text: ArrayExpress

characteristic: broker name

text: group 3 medulloblastoma

characteristic: clinical information

text: human

characteristic: common name

characteristic: description

text: juvenile stage

text: medulloblastoma

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: brain

text: Homo sapiens

text: DT-HDMB-03-CIS N = 3

characteristic: title


E-MTAB-12686:DT-D283-DMF N = 1

characteristic: ENA-CHECKLIST

text: NEWCASTLE UNIVERSITY

text: public

text: E-MTAB-12686:DT-D283-DMF N = 1

text: ArrayExpress

text: D283 Med

text: group 3 medulloblastoma

text: human

text: juvenile stage

characteristic: developmental stage

text: medulloblastoma

characteristic: disease

text: not applicable

characteristic: isolate

text: peritoneum

characteristic: metastatic site

text: Homo sapiens

text: Homo sapiens

text: brain

text: Homo sapiens

text: DT-D283-DMF N = 1


E-MTAB-12686:DT-D283-DMF N = 2

characteristic: ENA-FIRST-PUBLIC

text: NEWCASTLE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-12686:DT-D283-DMF N = 2

text: ArrayExpress

text: D283 Med

text: group 3 medulloblastoma

text: human

text: juvenile stage

text: medulloblastoma

text: not applicable

text: peritoneum

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: brain

text: Homo sapiens

text: DT-D283-DMF N = 2


E-MTAB-12686:DT-D283-DMF N = 3

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NEWCASTLE UNIVERSITY

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12686:DT-D283-DMF N = 3

text: ArrayExpress

text: D283 Med

text: group 3 medulloblastoma

text: human

text: juvenile stage

text: medulloblastoma

text: not applicable

text: peritoneum

text: Homo sapiens

text: Homo sapiens

text: brain

characteristic: organism part

text: Homo sapiens

text: DT-D283-DMF N = 3


E-MTAB-12686:DT-D458-DMF N = 1

text: NEWCASTLE UNIVERSITY

text: public

text: E-MTAB-12686:DT-D458-DMF N = 1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: group 3 medulloblastoma

characteristic: clinical information

text: human

characteristic: common name

characteristic: description

text: juvenile stage

text: medulloblastoma

text: not applicable

text: cerebrospinal fluid

text: Homo sapiens

text: Homo sapiens

text: brain

text: Homo sapiens

text: DT-D458-DMF N = 1


E-MTAB-12686:DT-D458-DMF N = 2

characteristic: ENA-CHECKLIST

text: NEWCASTLE UNIVERSITY

text: public

text: E-MTAB-12686:DT-D458-DMF N = 2

text: ArrayExpress

text: group 3 medulloblastoma

text: human

text: juvenile stage

characteristic: developmental stage

text: medulloblastoma

characteristic: disease

text: not applicable

characteristic: isolate

text: cerebrospinal fluid

text: Homo sapiens

text: Homo sapiens

text: brain

text: Homo sapiens

text: DT-D458-DMF N = 2

characteristic: title


E-MTAB-12686:DT-D458-DMF N = 3

characteristic: ENA-FIRST-PUBLIC

text: NEWCASTLE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-12686:DT-D458-DMF N = 3

text: ArrayExpress

text: group 3 medulloblastoma

text: human

text: juvenile stage

text: medulloblastoma

text: not applicable

text: cerebrospinal fluid

characteristic: metastatic site

text: Homo sapiens

text: Homo sapiens

text: brain

text: Homo sapiens

text: DT-D458-DMF N = 3


E-MTAB-12686:DT-HDMB-03-DMF N = 1

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NEWCASTLE UNIVERSITY

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12686:DT-HDMB-03-DMF N = 1

text: ArrayExpress

text: group 3 medulloblastoma

text: human

text: juvenile stage

text: medulloblastoma

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: brain

text: Homo sapiens

text: DT-HDMB-03-DMF N = 1


E-MTAB-12686:DT-HDMB-03-DMF N = 2

text: NEWCASTLE UNIVERSITY

text: public

text: E-MTAB-12686:DT-HDMB-03-DMF N = 2

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: group 3 medulloblastoma

characteristic: clinical information

text: human

characteristic: common name

text: juvenile stage

text: medulloblastoma

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: brain

characteristic: organism part

text: Homo sapiens

text: DT-HDMB-03-DMF N = 2


E-MTAB-12686:DT-HDMB-03-DMF N = 3

characteristic: ENA-CHECKLIST

text: NEWCASTLE UNIVERSITY

text: public

text: E-MTAB-12686:DT-HDMB-03-DMF N = 3

text: ArrayExpress

text: group 3 medulloblastoma

text: human

characteristic: description

text: juvenile stage

characteristic: developmental stage

text: medulloblastoma

characteristic: disease

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: brain

text: Homo sapiens

text: DT-HDMB-03-DMF N = 3


E-MTAB-12686:DT-DAOY-DMSO N = 1

characteristic: ENA-FIRST-PUBLIC

text: NEWCASTLE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-12686:DT-DAOY-DMSO N = 1

text: ArrayExpress

text: Daoy

text: SHH medulloblastoma

text: human

text: juvenile stage

text: desmoplastic medulloblastoma

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: brain

text: Homo sapiens

text: DT-DAOY-DMSO N = 1

characteristic: title


E-MTAB-12686:DT-DAOY-DMSO N = 2

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NEWCASTLE UNIVERSITY

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12686:DT-DAOY-DMSO N = 2

text: ArrayExpress

text: Daoy

text: SHH medulloblastoma

text: human

text: juvenile stage

text: desmoplastic medulloblastoma

text: not applicable

characteristic: metastatic site

text: Homo sapiens

text: Homo sapiens

text: brain

text: Homo sapiens

text: DT-DAOY-DMSO N = 2


E-MTAB-12686:DT-DAOY-DMSO N = 3

text: NEWCASTLE UNIVERSITY

text: public

text: E-MTAB-12686:DT-DAOY-DMSO N = 3

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: Daoy

text: SHH medulloblastoma

characteristic: clinical information

text: human

characteristic: common name

text: juvenile stage

text: desmoplastic medulloblastoma

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: brain

text: Homo sapiens

text: DT-DAOY-DMSO N = 3


characteristic: ENA-CHECKLIST

text: Vrije Universiteit Amsterdam

text: public

tag: Namespace:Vrije Universiteit Amsterdam

text: Tungara frog

text: DNA extracted from toe-clips

characteristic: description

text: Engystomops pustulosus

text: Engystomops pustulosus

text: AR_F_019 sample


characteristic: ENA-FIRST-PUBLIC

text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: AR_F_020 sample


text: Vrije Universiteit Amsterdam

characteristic: INSDC center name

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: AR_F_021 sample


text: Vrije Universiteit Amsterdam

characteristic: INSDC first public

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: AR_F_022 sample


text: Vrije Universiteit Amsterdam

characteristic: INSDC last update

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: AR_F_023 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: AR_F_024 sample

characteristic: title


characteristic: ENA-LAST-UPDATE

text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: AR_F_025 sample


characteristic: External Id

text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: AR_F_026 sample


text: Vrije Universiteit Amsterdam

text: public

characteristic: INSDC status

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: AR_F_027 sample


text: Vrije Universiteit Amsterdam

text: public

characteristic: SRA accession

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: AR_F_028 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: AR_F_029 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: AR_F_030 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: AR_F_031 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: AR_F_032 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: AR_U_001 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: AR_U_002 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: AR_U_003 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: AR_U_005 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: AR_U_006 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: AR_U_007 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: AR_U_008 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: AR_U_009 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: AR_U_010 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: AR_U_011 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: AR_U_012 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: AR_U_013 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: AR_U_014 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: AR_U_015 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: AR_U_016 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: AR_U_017 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: BG_F_051 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: BG_F_052 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: BG_F_053 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: BG_F_054 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: BG_F_055 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: BG_F_056 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: BG_F_057 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: BG_F_058 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: BG_F_059 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: BG_F_061 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: BG_F_062 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: BG_F_063 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: BG_F_064 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: BG_F_065 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: BG_F_160 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: BG_U_033 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: BG_U_034 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: BG_U_035 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: BG_U_036 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: BG_U_039 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: BG_U_040 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: BG_U_041 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: BG_U_042 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: BG_U_043 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: BG_U_044 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: BG_U_045 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: BG_U_047 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: BG_U_048 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: BG_U_049 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: BG_U_050 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: BG_U_159 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: CH_U_066 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: CH_U_067 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: CH_U_069 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: CH_U_070 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: CH_U_071 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: CH_U_072 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: CH_U_074 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: CH_U_078 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: CH_U_079 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: GM_U_082 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: GM_U_084 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: GM_U_096 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: GM_U_097 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: GM_U_098 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: GM_U_100 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: GM_U_161 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: GM_U_162 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: GM_U_163 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: GM_U_164 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: GM_U_165 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: GM_U_166 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: GM_U_167 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: GM_U_168 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: GM_U_169 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: GM_U_170 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: LC_F_101 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: LC_F_102 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: LC_F_103 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: LC_F_104 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: LC_F_105 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: LC_F_106 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: LC_F_107 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: LC_F_108 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PL_F_142 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PL_F_143 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PL_F_145 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PL_F_146 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PL_F_147 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PL_F_148 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PL_F_149 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PL_F_150 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PL_F_151 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PL_F_152 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PL_F_153 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PL_F_155 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PL_F_156 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PL_F_157 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PL_F_158 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PL_F_171 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PO_U_111 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PO_U_112 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PO_U_114 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PO_U_115 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PO_U_116 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PO_U_117 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PO_U_118 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PO_U_119 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PO_U_120 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PO_U_121 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PO_U_122 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PO_U_123 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PO_U_124 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PP_F_129 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PP_F_130 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PP_F_131 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PP_F_132 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PP_F_133 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PP_F_137 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PP_F_138 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PP_F_139 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PP_F_140 sample


text: Vrije Universiteit Amsterdam

text: public

text: Tungara frog

text: DNA extracted from toe-clips

text: Engystomops pustulosus

text: Engystomops pustulosus

text: PP_F_141 sample


characteristic: ENA first public

text: UNIVERSITY OF HAMBURG

text: public

tag: Namespace:UNIVERSITY OF HAMBURG

text: WGA of Daphnia resting eggs

text: Daphnia galeata

text: Daphnia galeata


text: UNIVERSITY OF HAMBURG

text: public

characteristic: Submitter Id

text: Daphnia longispina

text: Daphnia longispina


E-MTAB-12711:Sample 2

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12711:Sample 2

tag: Namespace:UNIVERSITY OF MANCHESTER

unit: year

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fibroblast

text: human

text: Protocols: The effects of the two candidate peptides was assessed by RNA-Seq analysis. Primary human dermal fibroblast cells from adult Caucasian females aged between 26-33 (taken from a range of skin sites: buttock (n=2), labia (n=2) and breast (n=1)) were seeded within appropriate cell densities into tissue culture plates in assay medium – Fibroblast Basal medium 2 (phenol red-free; PromoCell, C-23225) containing 2% FBS, 0.001% basic human fibroblast growth factor (hbFGF) and 0.005% insulin (Fibroblast growth medium 2 supplement pack; PromoCell, C39320)). Primary human dermal fibroblast cells from adult Caucasian females aged between 26-33 (taken from a range of skin sites: buttock (n=2), labia (n=2) and breast (n=1)) were seeded within appropriate cell densities into tissue culture plates in assay medium – Fibroblast Basal medium 2 (phenol red-free; PromoCell, C-23225) containing 2% FBS, 0.001% basic human fibroblast growth factor (hbFGF) and 0.005% insulin (Fibroblast growth medium 2 supplement pack; PromoCell, C39320)). After two days of culture in a humidified environment at 37°C with 5% CO2, once cells had reached confluency, cells were treated. Treatments included 8 parts per million (ppm) P1, 8ppm P7, a combination of P1 and P7 at 8 ppm each and 0.2 nM TGF-β1 (Gibco, PHG9214). RNA was extracted 12 hours following the treatment. RNA was extracted 12 hours following the treatment using the RNeasy Mini Kits (74104, Qiagen) according to the manufacturer's instructions. Briefly, lysis buffer was added to the samples, followed by precipitation of nucleic acids and desalting by addition of 70% ethanol. The resulting mixture was added to the RNeasy Mini Spin Columns and centrifuged to bind total RNA to the membrane. Samples were then washed, first with buffer RW1 to remove biomolecules such as carbohydrates, proteins and fatty acids which may have bound non-specifically to the membrane. The membrane was then washed twice with buffer RPE to remove salts from previous steps of the method. Finally, using RNAse-free water, total RNA was eluted from the spin columns. All extraction steps were performed on ice, with pre-chilled solutions. Following RNA extraction, RNA sample concentration was measured using a NanoDropTM Spectrophotometer (ThermoFisher Scientific). All samples were stored at -80˚C, and repetitive freeze-thaw cycles were avoided by aliquoting RNA samples. RNA samples were analysed as described below. Stranded mRNA assay (Illumina)

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: skin of breast

characteristic: organism part

text: Homo sapiens

text: female

characteristic: sex

text: Sample 2


E-MTAB-12711:Sample 5

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12711:Sample 5

text: ArrayExpress

text: fibroblast

text: human

characteristic: common name

characteristic: description

text: adult

text: normal

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: skin of breast

text: Homo sapiens

text: female

text: Sample 5


E-MTAB-12711:Sample 3

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12711:Sample 3

text: ArrayExpress

text: fibroblast

text: human

text: adult

text: normal

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin of breast

text: Homo sapiens

text: female

text: Sample 3

characteristic: title


E-MTAB-12711:Sample 4

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12711:Sample 4

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fibroblast

text: human

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: individual

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin of breast

text: Homo sapiens

text: female

text: Sample 4


E-MTAB-12711:Sample 6

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

text: public

text: E-MTAB-12711:Sample 6

text: ArrayExpress

text: fibroblast

text: human

text: adult

text: normal

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: skin of breast

characteristic: organism part

text: Homo sapiens

text: female

characteristic: sex

text: Sample 6


E-MTAB-12711:Sample 20

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC first public

text: public

text: E-MTAB-12711:Sample 20

text: ArrayExpress

text: fibroblast

text: human

characteristic: common name

characteristic: description

text: adult

text: normal

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: skin of buttock

text: Homo sapiens

text: female

text: Sample 20


E-MTAB-12711:Sample 23

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12711:Sample 23

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: fibroblast

text: human

text: adult

text: normal

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin of buttock

text: Homo sapiens

text: female

text: Sample 23

characteristic: title


E-MTAB-12711:Sample 21

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12711:Sample 21

text: ArrayExpress

characteristic: broker name

text: fibroblast

text: human

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: individual

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin of buttock

text: Homo sapiens

text: female

text: Sample 21


E-MTAB-12711:Sample 22

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

text: public

text: E-MTAB-12711:Sample 22

text: ArrayExpress

text: fibroblast

text: human

text: adult

text: normal

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: skin of buttock

characteristic: organism part

text: Homo sapiens

text: female

characteristic: sex

text: Sample 22


E-MTAB-12711:Sample 24

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12711:Sample 24

text: ArrayExpress

text: fibroblast

text: human

characteristic: common name

characteristic: description

text: adult

text: normal

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: skin of buttock

text: Homo sapiens

text: female

text: Sample 24


E-MTAB-12711:Sample 26

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

text: public

characteristic: SRA accession

text: E-MTAB-12711:Sample 26

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: fibroblast

text: human

text: adult

text: normal

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin of buttock

text: Homo sapiens

text: female

text: Sample 26

characteristic: title


E-MTAB-12711:Sample 29

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12711:Sample 29

text: ArrayExpress

characteristic: broker name

text: fibroblast

text: human

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: individual

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin of buttock

text: Homo sapiens

text: female

text: Sample 29


E-MTAB-12711:Sample 27

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-12711:Sample 27

text: ArrayExpress

text: fibroblast

text: human

text: adult

text: normal

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: skin of buttock

characteristic: organism part

text: Homo sapiens

text: female

characteristic: sex

text: Sample 27


E-MTAB-12711:Sample 28

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

text: public

characteristic: INSDC status

text: E-MTAB-12711:Sample 28

text: ArrayExpress

text: fibroblast

text: human

characteristic: common name

characteristic: description

text: adult

text: normal

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: skin of buttock

text: Homo sapiens

text: female

text: Sample 28


E-MTAB-12711:Sample 30

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

text: public

characteristic: SRA accession

text: E-MTAB-12711:Sample 30

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: fibroblast

text: human

text: adult

text: normal

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin of buttock

text: Homo sapiens

text: female

text: Sample 30

characteristic: title


E-MTAB-12711:Sample 8

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12711:Sample 8

text: ArrayExpress

characteristic: broker name

text: fibroblast

text: human

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: individual

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin of labia

text: Homo sapiens

text: female

text: Sample 8


E-MTAB-12711:Sample 11

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC last update

text: public

text: E-MTAB-12711:Sample 11

text: ArrayExpress

text: fibroblast

text: human

text: adult

text: normal

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: skin of labia

characteristic: organism part

text: Homo sapiens

text: female

characteristic: sex

text: Sample 11


E-MTAB-12711:Sample 9

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

text: public

characteristic: INSDC status

text: E-MTAB-12711:Sample 9

text: ArrayExpress

text: fibroblast

text: human

characteristic: common name

characteristic: description

text: adult

text: normal

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: skin of labia

text: Homo sapiens

text: female

text: Sample 9


E-MTAB-12711:Sample 10

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12711:Sample 10

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: fibroblast

text: human

text: adult

text: normal

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin of labia

text: Homo sapiens

text: female

text: Sample 10

characteristic: title


E-MTAB-12711:Sample 12

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12711:Sample 12

text: ArrayExpress

characteristic: broker name

text: fibroblast

text: human

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: individual

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin of labia

text: Homo sapiens

text: female

text: Sample 12


E-MTAB-12711:Sample 14

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC last update

text: public

text: E-MTAB-12711:Sample 14

text: ArrayExpress

text: fibroblast

text: human

text: adult

text: normal

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: skin of labia

characteristic: organism part

text: Homo sapiens

text: female

characteristic: sex

text: Sample 14


E-MTAB-12711:Sample 17

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: E-MTAB-12711:Sample 17

text: ArrayExpress

text: fibroblast

text: human

characteristic: common name

characteristic: description

text: adult

text: normal

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: skin of labia

text: Homo sapiens

text: female

text: Sample 17


E-MTAB-12711:Sample 15

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

text: public

characteristic: SRA accession

text: E-MTAB-12711:Sample 15

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: fibroblast

text: human

text: adult

text: normal

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin of labia

text: Homo sapiens

text: female

text: Sample 15

characteristic: title


E-MTAB-12711:Sample 16

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12711:Sample 16

text: ArrayExpress

characteristic: broker name

text: fibroblast

text: human

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: individual

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin of labia

text: Homo sapiens

text: female

text: Sample 16


E-MTAB-12711:Sample 18

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-12711:Sample 18

text: ArrayExpress

text: fibroblast

text: human

text: adult

text: normal

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: skin of labia

characteristic: organism part

text: Homo sapiens

text: female

characteristic: sex

text: Sample 18


E-MTAB-12710:Sample 1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

text: public

characteristic: INSDC status

text: E-MTAB-12710:Sample 1

text: ArrayExpress

text: fibroblast

text: human

characteristic: common name

text: Protocols: The effects of the peptide blend was assessed by RNA-Seq analysis. Primary human dermal fibroblast cells from adult Caucasian females aged between 23-27 (taken from buttock (n=3)) were seeded within appropriate cell densities into tissue culture plates in assay medium – Fibroblast Basal medium 2 (phenol red-free; PromoCell, C-23225) containing 2% FBS, 0.001% basic human fibroblast growth factor (hbFGF) and 0.005% insulin (Fibroblast growth medium 2 supplement pack; PromoCell, C39320)). Primary human dermal fibroblast cells from adult Caucasian females aged between 23-27 (taken from buttock (n=3)) were seeded within appropriate cell densities into tissue culture plates in assay medium – Fibroblast Basal medium 2 (phenol red-free; PromoCell, C-23225) containing 2% FBS, 0.001% basic human fibroblast growth factor (hbFGF) and 0.005% insulin (Fibroblast growth medium 2 supplement pack; PromoCell, C39320)). After two days of culture in a humidified environment at 37°C with 5% CO2, once cells had reached confluency, cells were either left untreated as a negative control, or treated with a combination of P1 and P7 at 8 parts per million (ppm) each, or 1 μM ATRA (Sigma-Aldrich, R2625). All treatments were performed in triplicate. RNA was extracted 12 hours following the treatment using the RNeasy Mini Kits (74104, Qiagen) according to the manufacturer's instructions. Briefly, lysis buffer was added to the samples, followed by precipitation of nucleic acids and desalting by addition of 70% ethanol. The resulting mixture was added to the RNeasy Mini Spin Columns and centrifuged to bind total RNA to the membrane. Samples were then washed, first with buffer RW1 to remove biomolecules such as carbohydrates, proteins and fatty acids which may have bound non-specifically to the membrane. The membrane was then washed twice with buffer RPE to remove salts from previous steps of the method. Finally, using RNAse-free water, total RNA was eluted from the spin columns. All extraction steps were performed on ice, with pre-chilled solutions. Following RNA extraction, RNA sample concentration was measured using a NanoDropTM Spectrophotometer (ThermoFisher Scientific). All samples were stored at -80˚C, and repetitive freeze-thaw cycles were avoided by aliquoting RNA samples. RNA samples were analysed as described below. Stranded mRNA assay (Illumina)

characteristic: description

text: adult

text: normal

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: skin from buttock

text: Homo sapiens

text: female

text: Sample 1


E-MTAB-12710:Sample 4

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

text: public

characteristic: SRA accession

text: E-MTAB-12710:Sample 4

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: fibroblast

text: human

text: adult

text: normal

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin from buttock

text: Homo sapiens

text: female

text: Sample 4

characteristic: title


E-MTAB-12710:Sample 7

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12710:Sample 7

text: ArrayExpress

characteristic: broker name

text: fibroblast

text: human

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: individual

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin from buttock

text: Homo sapiens

text: female

text: Sample 7


E-MTAB-12710:Sample 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC last update

text: public

text: E-MTAB-12710:Sample 2

text: ArrayExpress

text: fibroblast

text: human

text: adult

text: normal

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: skin from buttock

characteristic: organism part

text: Homo sapiens

text: female

characteristic: sex

text: Sample 2


E-MTAB-12710:Sample 5

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

text: public

characteristic: INSDC status

text: E-MTAB-12710:Sample 5

text: ArrayExpress

text: fibroblast

text: human

characteristic: common name

characteristic: description

text: adult

text: normal

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: skin from buttock

text: Homo sapiens

text: female

text: Sample 5


E-MTAB-12710:Sample 8

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12710:Sample 8

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: fibroblast

text: human

text: adult

text: normal

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin from buttock

text: Homo sapiens

text: female

text: Sample 8

characteristic: title


E-MTAB-12710:Sample 3

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

text: public

text: E-MTAB-12710:Sample 3

text: ArrayExpress

characteristic: broker name

text: fibroblast

text: human

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: individual

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin from buttock

text: Homo sapiens

text: female

text: Sample 3


E-MTAB-12710:Sample 6

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC last update

text: public

text: E-MTAB-12710:Sample 6

text: ArrayExpress

text: fibroblast

text: human

text: adult

text: normal

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: skin from buttock

characteristic: organism part

text: Homo sapiens

text: female

characteristic: sex

text: Sample 6


E-MTAB-12710:Sample 9

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: E-MTAB-12710:Sample 9

text: ArrayExpress

text: fibroblast

text: human

characteristic: common name

characteristic: description

text: adult

text: normal

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: skin from buttock

text: Homo sapiens

text: female

text: Sample 9


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: SRA accession

tag: Namespace:Institut Necker Enfants Malades (INEM), INSERM U1151/CNRS UMS 8253, Universite Paris Cite, Paris, France.

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: B cell

text: mRNA vaccinated

characteristic: clinical information

text: human

text: Protocols: For each sample, we always FACS sorted (MA900, Sony) an initial pool of 50.000 total peripheral CD3-CD14-CD56- CD19+ IgD- cells and afterward, to enrich for cells of interest, only sorted CD19+CD38low antibody secreting cells (ASCs), CD19hi IgD+ cells and SARS-CoV-2 Spike/RBD PE/tetramer positive B cells, leading to approximately 55000-60000 total sorted cells per sample. Inferred cell types per sample can be found in the corresponding sample_information_filtered.tsv file. Cells were counted and up to 20 000 cells were loaded in the 10x Chromium Controller to generate single-cell gel-beads in emulsion. After reverse transcription, gel-beads in emulsion were disrupted. Barcoded complementary DNA was isolated and amplified by PCR. The scRNA-seq libraries were generated using Chromium Next GEM Single Cell V(D)J Reagent Kit v.1.1 with Feature Barcoding (10x Genomics) according to the manufacturer’s protocol. Gene expression (mRNA), ADT and VDJ BCR libraries were constructed.

text: adult

text: Severe acute respiratory syndrome coronavirus 2 BA.1 Omicron

characteristic: infect

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: male

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Institut Necker Enfants Malades (INEM), INSERM U1151/CNRS UMS 8253, Universite Paris Cite, Paris, France.

characteristic: INSDC last update

text: public

text: ArrayExpress

characteristic: broker name

text: B cell

text: mRNA vaccinated

text: human

text: adult

characteristic: developmental stage

characteristic: disease

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: male


characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: B cell

text: mRNA vaccinated

text: human

characteristic: common name

characteristic: description

text: adult

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens

text: female

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: SRA accession

tag: Namespace:Natural Resources Institute Finland (Luke)

characteristic: Submitter Id

unit: day

characteristic: age

text: ArrayExpress

text: domesticated barley

text: GoldenPromise

characteristic: cultivar

text: Protocols: Second leaves from the youngest tillers were collected for RNA analysis: Timepoint 1 (T1) on the last day of the well-watered phase (day 12); T2, on the last day of Dry Phase II (day 41); T3, last day of Rewatering II (day 44). Well-watered control samples at the same growth stages were collected at T2 and T3. For RNA extraction, leaf samples were collected in liquid N2 and later ground and stored in TRIzol reagent (Invitrogen). Total RNA was extracted from the leaf samples with the Direct-zol RNA Miniprep Plus kit according to the manufacturer’s (Zymo Research Europe GmbH) guidelines. RNA (1 µg) from each sample was treated with Ambion® DNase I (RNase-free; Applied Biosystems) in a 20 µl reaction mixture for 90 min at 37 °C to remove remaining DNA. An RNeasy MinElute Cleanup Kit (Qiagen) was used to purify and concentrate the RNA from the DNase treatments. RNA quantity and quality were checked both spectrophotometrically (NANODROP-2000 spectrophotometer, Thermo Scientific) and by agarose gel electrophoresis. Plants grown during the winter season in the iCORE functional-phenotyping greenhouse (https://plantscience.agri.huji.ac.il/icore-center) under natural light, with moderate temperature control, in Rehovot, Israel. Seeds were sown in germination trays, then transplanted at the 3-4-leaf stage (two weeks old) to pots. Before transplanting, seedling roots were carefully to remove the original soil, planted into potting soil (“Bental 11”, Tuff Marom Golan, Israel), four per 3.9-litre pot, then placed onto lysimeters. The plants were grown at 18–25°C, 20–35% relative humidity, and 10h light /14h dark. The vapor pressure deficit (VPD) ranged between 0.2 to 4.0 kPa in the greenhouse and represented typical weather fluctuations during winters in Central Israel Drought experiments were carried out on a gravimetric functional phenotyping platform (Dalal A et al. 2020. A Telemetric, Gravimetric Platform for Real-Time Physiological Phenotyping of Plant-Environment Interactions. J Vis Exp. DOI: 10.3791/61280). The experiment was divided into five phases on the lysimeter platform: (i) well-watered; (ii) Dry Phase I (iii); Rewatering I (iv); Dry Phase II; (v) Rewatering II. For the well-watered phase, all plants received daily night irrigation for the first 12 days with three irrigations in 3-hour intervals. In Dry Phase I, the system was set to irrigate each plant to 80% of its own previous day’s transpiration, so that all plants would be subjected to the same gradual water stress from day 13 to day 24, followed by rewatering (Rewatering I) on the night of day 25. Dry Phase II extended from day 26 to day 41 with the same irrigation parameters as Dry Phase I, until the time when the droughted plants reached <100g daily transpiration (around 20 to 30% of controls). Rewatering II began on day 42. Daily night irrigation was given to all plants until day 44. On the first irrigation, several irrigation cycles were used to moisten the soil and to ensure a laterally uniform soil water content (SWC) in the pots. VPD data were taken daily at 17:22 from the SPAC analysis. Total RNA was extracted from the leaf samples with the Direct-zol RNA Miniprep Plus kit according to the manufacturer’s (Zymo Research Europe GmbH) guidelines. RNA (1 µg) from each sample was treated with Ambion® DNase I (RNase-free; Applied Biosystems) in a 20 µl reaction mixture for 90 min at 37 °C to remove remaining DNA. An RNeasy MinElute Cleanup Kit (Qiagen) was used to purify and concentrate the RNA from the DNase treatments. RNA quantity and quality were checked both spectrophotometrically (NANODROP-2000 spectrophotometer, Thermo Scientific) and by agarose gel electrophoresis. Libraries were constructed using the TruSeq Stranded Total RNA kit; adaptors were TruSeq adaptors. Cytoplasmic and chloroplast was removed by hybridization on beads. Following rRNA removal, the RNA was fragmented into small pieces using divalent cations under elevated temperature. Cleaved RNA fragments were copied into first strand cDNA using reverse transcriptase and random primers.

text: pre-flowering

text: somatic genotype

characteristic: genotype

text: pots in greenhouse, natural light

characteristic: growth condition

text: not applicable

text: Hordeum vulgare subsp. vulgare

text: Hordeum vulgare subsp. vulgare

text: leaf

text: Hordeum vulgare subsp. vulgare

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Natural Resources Institute Finland (Luke)

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: ArrayExpress

characteristic: broker name

text: domesticated barley

text: GoldenPromise

text: pre-flowering

characteristic: developmental stage

text: somatic genotype

text: pots in greenhouse, natural light

text: not applicable

characteristic: isolate

text: Hordeum vulgare subsp. vulgare

text: Hordeum vulgare subsp. vulgare

text: leaf

text: Hordeum vulgare subsp. vulgare


characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: domesticated barley

characteristic: common name

text: GoldenPromise

characteristic: description

text: pre-flowering

text: somatic genotype

text: pots in greenhouse, natural light, drought at day 41

text: not applicable

text: Hordeum vulgare subsp. vulgare

characteristic: organism

text: Hordeum vulgare subsp. vulgare

text: leaf

characteristic: organism part

text: Hordeum vulgare subsp. vulgare

text: GPD


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: domesticated barley

text: GoldenPromise

characteristic: cultivar

text: pre-flowering

text: somatic genotype

text: pots in greenhouse, natural light, rewatering at day 44ht

text: not applicable

text: Hordeum vulgare subsp. vulgare

text: Hordeum vulgare subsp. vulgare

text: leaf

text: Hordeum vulgare subsp. vulgare

text: GPR


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NTNU University Museum

characteristic: INSDC center name

text: public

tag: Namespace:NTNU University Museum

text: Reindeer sample MLM12

text: Rangifer tarandus platyrhyncus

text: Rangifer tarandus platyrhyncus

characteristic: title


text: NTNU University Museum

characteristic: INSDC last update

text: public

text: Reindeer sample M72

text: Rangifer tarandus platyrhyncus

text: Rangifer tarandus platyrhyncus


text: NTNU University Museum

characteristic: INSDC first public

text: public

text: Reindeer sample MLM51

text: Rangifer tarandus platyrhyncus

text: Rangifer tarandus platyrhyncus


text: NTNU University Museum

text: public

characteristic: INSDC status

text: Reindeer sample MLM82

text: Rangifer tarandus platyrhyncus

text: Rangifer tarandus platyrhyncus


text: NTNU University Museum

text: public

text: Reindeer sample B20

text: Rangifer tarandus platyrhyncus

text: Rangifer tarandus platyrhyncus


text: NTNU University Museum

text: public

text: Reindeer sample MLM16

text: Rangifer tarandus platyrhyncus

text: Rangifer tarandus platyrhyncus


text: NTNU University Museum

text: public

text: Reindeer sample MLM80

text: Rangifer tarandus platyrhyncus

text: Rangifer tarandus platyrhyncus


text: NTNU University Museum

text: public

text: Reindeer sample BBH7

text: Rangifer tarandus platyrhyncus

text: Rangifer tarandus platyrhyncus


text: NTNU University Museum

text: public

text: Reindeer sample M44

text: Rangifer tarandus platyrhyncus

text: Rangifer tarandus platyrhyncus


text: NTNU University Museum

text: public

text: Reindeer sample M46

text: Rangifer tarandus platyrhyncus

text: Rangifer tarandus platyrhyncus


text: NTNU University Museum

text: public

text: Reindeer sample M68

text: Rangifer tarandus platyrhyncus

text: Rangifer tarandus platyrhyncus


text: NTNU University Museum

text: public

text: Reindeer sample MB60

text: Rangifer tarandus platyrhyncus

text: Rangifer tarandus platyrhyncus


text: NTNU University Museum

text: public

text: Reindeer sample MDV11

text: Rangifer tarandus platyrhyncus

text: Rangifer tarandus platyrhyncus


text: NTNU University Museum

text: public

text: Reindeer sample MLM61

text: Rangifer tarandus platyrhyncus

text: Rangifer tarandus platyrhyncus


text: NTNU University Museum

text: public

text: Reindeer sample R20

text: Rangifer tarandus platyrhyncus

text: Rangifer tarandus platyrhyncus


text: NTNU University Museum

text: public

text: Reindeer sample R25a

text: Rangifer tarandus platyrhyncus

text: Rangifer tarandus platyrhyncus


text: NTNU University Museum

text: public

text: Reindeer sample R29a

text: Rangifer tarandus platyrhyncus

text: Rangifer tarandus platyrhyncus