characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Centre for Genomic Regulation (CRG)

text: public

text: ArrayExpress

text: missing: third party data

characteristic: collection date

text: human

text: adult

text: normal

characteristic: geographic location (country and/or sea)

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: heart

text: Homo sapiens

text: male

text: pacBio-Cshl-CapTrap-Heart

characteristic: title


status: PUBLIC

tag: attribute

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Centre for Genomic Regulation (CRG)

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13063:pacBioSII-Cshl-CapTrap-Brain

tag: Namespace:Centre for Genomic Regulation (CRG)

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: missing: third party data

text: human

characteristic: common name

text: Protocols: commercial samples obtained from Ambion: Ambion is a brand of life science research products and technologies, mainly focused on RNA products. It is owned by Thermo Fisher Scientific, a company that provides a variety of scientific research products and services. Therefore, \"total RNA commercial samples obtained from Ambion\" refers to RNA samples purchased from this brand for research purposes. CapTrap cDNA library preparation: CapTrap cDNA library preparation is a long-read sequencing protocol that selectively enriches 5'-capped transcripts by capturing the 5' caps of transcripts. This protocol uses Poly(A) tail selection to specifically target mRNA, and a 5' cap-specific oxidation reaction coupled to biotin-streptavidin to capture the 5' cap structure. The captured mRNA is then converted to cDNA and the library is prepared with 5' and 3' specific linkers to enrich for full-length libraries. CapTrap is particularly useful for studying transcription start sites, alternative splicing, and post-transcriptional modifications.

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: missing: data agreement established pre-2023

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: brain

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex

text: pacBioSII-Cshl-CapTrap-Brain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Centre for Genomic Regulation (CRG)

text: public

text: E-MTAB-13063:pacBioSII-Cshl-CapTrap-Heart

text: ArrayExpress

text: missing: third party data

characteristic: collection date

text: human

text: adult

text: normal

characteristic: geographic location (country and/or sea)

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: heart

text: Homo sapiens

text: male

text: pacBioSII-Cshl-CapTrap-Heart

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: German Cancer Research Center

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

tag: Namespace:German Cancer Research Center

text: ArrayExpress

text: not provided

text: house mouse

text: Protocols: Mice were euthanized by increasing concentrations of carbon dioxide. Single-cell suspensions from spleens were prepared using the GentleMacs system (Miltenyi Biotech, Bergisch Gladbach, Germany). Splenocytes were enriched in TCL1 cells using EasySep™ Mouse Pan-B Cell Isolation Kit (Stemcell Technologies, Vancouver, Canada) for further analyses, resulting in a purity above 97% of CD5+ CD19+ cells. Single-cell suspensions from spleens were prepared using the GentleMacs system (Miltenyi Biotech, Bergisch Gladbach, Germany). Splenocytes were enriched in TCL1 cells using EasySep™ Mouse Pan-B Cell Isolation Kit (Stemcell Technologies, Vancouver, Canada) for further analyses, resulting in a purity above 97% of CD5+ CD19+ cells, or cryopreserved and thawed when necessary. RNA isolation was performed according to manufacturer’s recommendations using QIAshredder and RNeasy Mini Kit (both Qiagen, Hilden, Germany). For RNA sequencing libraries were prepared using the Illumina TruSeq Stranded mRNA Kit.

text: Tbx21 knockout

characteristic: genetic modification

characteristic: genotype

text: not provided

text: CD5+ CD19+ TCL1

characteristic: immunophenotype

text: not applicable

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: female

characteristic: strain


text: German Cancer Research Center

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: not provided

text: house mouse

characteristic: common name

characteristic: description

text: not provided

text: CD5+ CD19+ TCL1

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: spleen

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Pasteur Institute

text: public

text: ena-SAMPLE-TAB-09-06-2023-15:17:15:148-59801

tag: Namespace:Pasteur Institute

text: not provided

characteristic: collection date

text: France

characteristic: geographic location (country and/or sea)

text: not applicable

characteristic: host health state

characteristic: host scientific name

text: Vibrio cholerae O1 biovar El Tor str. N16961

characteristic: organism

characteristic: serovar

text: ChIP in a wt Vibrio cholerae strain with no FLAG protein (negative control)

characteristic: title


text: Pasteur Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-09-06-2023-15:17:15:148-59803

characteristic: Submitter Id

text: not provided

text: France

text: not applicable

characteristic: strain

text: ChIPseq anti-FLAG in Vibrio cholerae parB2-3xFLAG (tagged on the chromosomal locus) strain (INPUT)


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Pasteur Institute

text: public

text: ena-SAMPLE-TAB-09-06-2023-15:17:15:148-59805

text: not provided

text: France

text: not applicable

characteristic: isolate

text: ChIPseq anti-FLAG in Vibrio cholerae parB2-3xFLAG (tagged on the chromosomal locus) strain (IP)


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Pasteur Institute

text: public

text: ena-SAMPLE-TAB-09-06-2023-15:17:15:149-59807

text: not provided

characteristic: collection date

text: France

text: not applicable

text: ChIPseq anti-FLAG in Vibrio cholerae 29merC/A RctB-3xFLAG (tagged on the chromosomal locus) strain (INPUT)


text: Pasteur Institute

text: public

text: ena-SAMPLE-TAB-09-06-2023-15:17:15:149-59809

text: not provided

text: France

characteristic: geographic location (country and/or sea)

text: not applicable

characteristic: host health state

characteristic: host scientific name

text: ChIPseq anti-FLAG in Vibrio cholerae 29merC/A RctB-3xFLAG (tagged on the chromosomal locus) strain (IP)


text: Pasteur Institute

text: public

text: ena-SAMPLE-TAB-09-06-2023-15:17:15:149-59819

text: not provided

text: France

text: not applicable

characteristic: organism

characteristic: serovar

text: ChIPseq anti-FLAG in Vibrio cholerae �crtS RctB-3xFLAG (tagged on the chromosomal locus) strain (INPUT)

characteristic: title


text: Pasteur Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ena-SAMPLE-TAB-09-06-2023-15:17:15:149-59821

text: not provided

text: France

text: not applicable

text: ChIPseq anti-FLAG in Vibrio cholerae �crtS RctB-3xFLAG (tagged on the chromosomal locus) strain (IP)


text: Pasteur Institute

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-09-06-2023-15:17:15:149-59823

characteristic: Submitter Id

text: not provided

text: France

text: not applicable

text: ChIPseq anti-FLAG in Vibrio cholerae RctB-3xFLAG (tagged on the chromosomal locus) strain exponential phase (INPUT)


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Pasteur Institute

text: public

text: ena-SAMPLE-TAB-09-06-2023-15:17:15:150-59825

text: not provided

text: France

text: not applicable

characteristic: strain

text: ChIPseq anti-FLAG in Vibrio cholerae RctB-3xFLAG (tagged on the chromosomal locus) strain exponential phase (IP)


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Pasteur Institute

text: public

text: ena-SAMPLE-TAB-09-06-2023-15:17:15:150-59827

text: not provided

text: France

text: not applicable

characteristic: isolate

text: Vibrio cholerae O1 biovar El Tor str. N16961

text: ChIPseq anti-FLAG in Vibrio cholerae RctB-L651P-3xFLAG (tagged on the chromosomal locus) strain (INPUT)


text: Pasteur Institute

text: public

text: ena-SAMPLE-TAB-09-06-2023-15:17:15:150-59829

text: not provided

characteristic: collection date

text: France

characteristic: geographic location (country and/or sea)

text: not applicable

characteristic: host health state

text: ChIPseq anti-FLAG in Vibrio cholerae RctB-L651P-3xFLAG (tagged on the chromosomal locus) strain (IP)


text: Pasteur Institute

text: public

text: ena-SAMPLE-TAB-09-06-2023-15:17:15:150-59831

text: not provided

text: France

text: not applicable

characteristic: host scientific name

characteristic: organism

characteristic: serovar

text: ChIPseq anti-FLAG in Vibrio cholerae RctB-3xFLAG (tagged on the chromosomal locus) strain 0min after SHX removal (INPUT)


text: Pasteur Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ena-SAMPLE-TAB-09-06-2023-15:17:15:150-59833

text: not provided

text: France

text: not applicable

text: ChIPseq anti-FLAG in Vibrio cholerae RctB-3xFLAG (tagged on the chromosomal locus) strain 0min after SHX removal (IP)

characteristic: title


text: Pasteur Institute

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-09-06-2023-15:17:15:150-59835

text: not provided

text: France

text: not applicable

text: ChIPseq anti-FLAG in Vibrio cholerae RctB-3xFLAG (tagged on the chromosomal locus) strain 15min after SHX removal (INPUT)


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Pasteur Institute

text: public

text: ena-SAMPLE-TAB-09-06-2023-15:17:15:150-59837

characteristic: Submitter Id

text: not provided

text: France

text: not applicable

text: ChIPseq anti-FLAG in Vibrio cholerae RctB-3xFLAG (tagged on the chromosomal locus) strain 15min after SHX removal (IP)


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Pasteur Institute

text: public

text: ena-SAMPLE-TAB-09-06-2023-15:17:15:150-59839

text: not provided

text: France

text: not applicable

characteristic: strain

text: ChIPseq anti-FLAG in Vibrio cholerae RctB-3xFLAG (tagged on the chromosomal locus) strain 30min after SHX removal (INPUT)


text: Pasteur Institute

text: public

text: ena-SAMPLE-TAB-09-06-2023-15:17:15:150-59841

text: not provided

characteristic: collection date

text: France

characteristic: geographic location (country and/or sea)

text: not applicable

characteristic: isolate

text: ChIPseq anti-FLAG in Vibrio cholerae RctB-3xFLAG (tagged on the chromosomal locus) strain 30min after SHX removal (IP)


text: Pasteur Institute

text: public

text: ena-SAMPLE-TAB-09-06-2023-15:17:15:151-59843

text: not provided

text: France

text: not applicable

characteristic: host health state

characteristic: host scientific name

characteristic: organism

text: ChIPseq anti-FLAG in Vibrio cholerae RctB-3xFLAG (tagged on the chromosomal locus) strain 45min after SHX removal (INPUT)


text: Pasteur Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ena-SAMPLE-TAB-09-06-2023-15:17:15:151-59845

text: not provided

text: France

text: not applicable

text: Vibrio cholerae O1 biovar El Tor str. N16961

characteristic: serovar

text: ChIPseq anti-FLAG in Vibrio cholerae RctB-3xFLAG (tagged on the chromosomal locus) strain 45min after SHX removal (IP)


text: Pasteur Institute

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-09-06-2023-15:17:15:151-59847

text: not provided

text: France

text: not applicable

text: ChIPseq anti-FLAG in Vibrio cholerae RctB-3xFLAG (tagged on the chromosomal locus) strain 60min after SHX removal (INPUT)

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Pasteur Institute

text: public

characteristic: SRA accession

text: ena-SAMPLE-TAB-09-06-2023-15:17:15:151-59849

text: not provided

text: France

text: not applicable

text: ChIPseq anti-FLAG in Vibrio cholerae RctB-3xFLAG (tagged on the chromosomal locus) strain 60min after SHX removal (IP)


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Pasteur Institute

text: public

text: ena-SAMPLE-TAB-09-06-2023-15:17:15:151-59851

characteristic: Submitter Id

text: not provided

text: France

text: not applicable

text: ChIPseq anti-FLAG in Vibrio cholerae RctB-3xFLAG (tagged on the chromosomal locus) strain stationnary phase (INPUT)


text: Pasteur Institute

text: public

text: ena-SAMPLE-TAB-09-06-2023-15:17:15:151-59853

text: not provided

characteristic: collection date

text: France

characteristic: geographic location (country and/or sea)

text: not applicable

characteristic: strain

text: ChIPseq anti-FLAG in Vibrio cholerae RctB-3xFLAG (tagged on the chromosomal locus) strain stationnary phase (IP)


text: Aix-Marseille University

text: public

text: ena-SAMPLE-TAB-13-06-2023-07:37:11:563-3991

tag: Namespace:Aix-Marseille University

text: D. ramea from Cyprus

characteristic: description

text: Cyprus

text: Dendrophyllia ramea

text: Dendrophyllia ramea

text: D-ramea-MON-A


text: Aix-Marseille University

text: public

text: ena-SAMPLE-TAB-13-06-2023-07:37:11:563-3992

text: D. ramea from Cyprus

text: Cyprus

text: Dendrophyllia ramea

characteristic: organism

text: Dendrophyllia ramea

text: D-ramea-MON-B


text: Aix-Marseille University

characteristic: INSDC center name

text: public

text: ena-SAMPLE-TAB-13-06-2023-07:37:11:563-3993

text: D. cornigera from Balearic Islands

text: Spain

text: Dendrophyllia cornigera

text: Dendrophyllia cornigera

text: D-corn-BAL-A


text: Aix-Marseille University

characteristic: INSDC first public

text: public

text: ena-SAMPLE-TAB-13-06-2023-07:37:11:563-3994

text: D. cornigera from Balearic Islands

text: Spain

text: Dendrophyllia cornigera

text: Dendrophyllia cornigera

text: D-corn-BAL-B


text: Aix-Marseille University

text: public

text: ena-SAMPLE-TAB-13-06-2023-07:37:11:563-3995

text: not provided

text: D. cornigera from Lavaze-Duthiers canyon kept at Banyuls aquarium, noted ramea by error

text: France

text: Dendrophyllia cornigera

text: Dendrophyllia cornigera

text: D-ramea-BAN-A


text: Aix-Marseille University

text: public

text: ena-SAMPLE-TAB-13-06-2023-07:37:11:564-3996

text: Saint-Dominique Wreck

text: France

text: Caryophyllia inornata

text: Caryophyllia inornata

text: C-inorn-SDO-A


text: Aix-Marseille University

text: public

text: ena-SAMPLE-TAB-13-06-2023-07:37:11:564-3997

text: Saint-Dominique Wreck

text: France

text: Caryophyllia inornata

text: Caryophyllia inornata

text: C-inorn-SDO-B


text: Aix-Marseille University

text: public

text: ena-SAMPLE-TAB-13-06-2023-07:37:11:564-3998

text: Saint-Dominique Wreck

text: France

text: Caryophyllia inornata

text: Caryophyllia inornata

text: C-inorn-SDO-C


text: Aix-Marseille University

text: public

text: ena-SAMPLE-TAB-13-06-2023-07:37:11:564-3999

text: Saint-Dominique Wreck

text: France

text: Caryophyllia smithii

text: Caryophyllia smithii

text: C-smith-SDO-A


text: Aix-Marseille University

text: public

text: ena-SAMPLE-TAB-13-06-2023-07:37:11:564-4000

text: Saint-Dominique Wreck

text: France

text: Caryophyllia smithii

text: Caryophyllia smithii

text: C-smith-SDO-B


text: Aix-Marseille University

text: public

text: ena-SAMPLE-TAB-13-06-2023-07:37:11:564-4001

text: Saint-Dominique Wreck

text: France

text: Caryophyllia smithii

text: Caryophyllia smithii

text: C-smith-SDO-C


text: Centro Nacional de Análisis Genómico

characteristic: GAL

text: Not collected

text: EarlhamInstitute

text: public

text: COPO

characteristic: broker name

text: MARC VENTURA

text: CENTER FOR ADVANCED STUDIES OF BLANES (CEAB-CSIC)

characteristic: collecting institution

characteristic: collector ORCID ID

unit: m

characteristic: elevation

text: Spain

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: CATALONIA|EBRO DELTA, ICHTYOLOGICAL INSTITUTE

characteristic: geographic location (region and locality)

text: CULTURE

characteristic: habitat

text: Nati Franch

text: Delta de l'Ebre Natural Park

text: adult

characteristic: lifestage

text: Valencia hispanica

text: LIVER

characteristic: organism part

text: SPAIN/CATALONIA/LLACUNES DE SANTES CREUS

characteristic: original collection location

characteristic: original geographic location (latitude)

characteristic: original geographic location (longitude)

text: ERGA

characteristic: project name

text: NOT PROVIDED

characteristic: proxy voucher

text: caught with fyke net

characteristic: sample collection device or method

text: Javier Palma Guerrero | Rosa Fernandez

characteristic: sample coordinator

characteristic: sample coordinator ORCID ID

text: INSTITUTE OF EVOLUTIONARY BIOLOGY | INSTITUTE OF EVOLUTIONARY BIOLOGY

characteristic: sample coordinator affiliation

characteristic: sample derived from

text: Valencia hispanica

text: FEMALE

characteristic: sex

text: ERGA MV 414X 001

text: MNCN ICTIO 296.954

text: 9d3c03d7-cc09-456e-8a75-dc0a58344e79-erga

characteristic: tolid


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: STATENS SERUM INSTITUT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:STATENS SERUM INSTITUT

characteristic: Submitter Id

characteristic: collection date

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

text: Staphylococcus aureus

characteristic: organism

text: Staphylococcus aureus

characteristic: title


text: UPPSALA UNIVERSITET

text: public

tag: Namespace:UPPSALA UNIVERSITET

text: not provided

text: aurochs

characteristic: common name

text: Bos remain excavated from Artusia

text: Spain

text: 42.67, -1.61

text: Bos primigenius

text: Bos primigenius


text: UPPSALA UNIVERSITET

text: public

text: not provided

text: aurochs

text: Bos remain excavated from Els Trocs

text: Spain

text: 42.5, 0.5

text: Bos primigenius

text: Bos primigenius


text: UPPSALA UNIVERSITET

text: public

text: not provided

text: cattle

text: Bos remain excavated from El Portalón

text: Spain

text: 42.35, -3.52

text: Bos taurus

text: Bos taurus


text: UPPSALA UNIVERSITET

text: public

text: not provided

text: aurochs

text: Bos remain excavated from El Portalón

text: Spain

text: 42.35, -3.52

text: Bos primigenius

text: Bos primigenius


text: UPPSALA UNIVERSITET

text: public

text: not provided

text: cattle

text: Bos remain excavated from Mendandia

text: Spain

text: 42.74, -2.59

text: Bos taurus

text: Bos taurus


text: UPPSALA UNIVERSITET

text: public

text: not provided

text: aurochs

text: Bos remain excavated from Mendandia

text: Spain

text: 42.74, -2.59

text: Bos primigenius

text: Bos primigenius


text: public

tag: Namespace:Institute of Molecular Genetics of the Czech Academy of Sciences

unit: week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: not collected

text: house mouse

text: Protocols: Long-term HSCs (LT-HSCs sorted from 12-week-old WT and Ppm1DT/+ mice using Influx instrument (BD Biosciences). RNA was isolated from c-Kit+ cells isolated from the leukemic animals using KAPA mRNA HyperPrep Kit (Kapa Biosystems). Library was prepared using Illumina’s KAPA mRNA Hyperprep Kit as per manufactures instructio

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: wild type genotype

characteristic: genotype

text: not collected

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: bone marrow

characteristic: organism part

characteristic: replicate

text: Mus musculus

text: male

characteristic: sex

text: genotoxic stress

characteristic: stimulus

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Institute of Molecular Genetics of the Czech Academy of Sciences

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: not collected

characteristic: collection date

text: house mouse

text: adult

text: acute myeloid leukemia

text: wild type genotype

text: not collected

text: not applicable

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: male

text: genotoxic stress


text: public

characteristic: age

text: ArrayExpress

characteristic: broker name

text: not collected

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: wild type genotype

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

characteristic: isolate

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

text: genotoxic stress

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: not collected

text: house mouse

text: adult

text: acute myeloid leukemia

text: Ppm1d T/+

text: not collected

text: not applicable

text: Mus musculus

text: Mus musculus

text: bone marrow

characteristic: organism part

characteristic: replicate

text: Mus musculus

text: male

characteristic: sex

text: genotoxic stress

characteristic: stimulus

characteristic: strain


characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: Ppm1d T/+

text: not collected

text: not applicable

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

text: genotoxic stress


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: not collected

text: house mouse

text: adult

text: normal

text: wild type genotype

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

characteristic: isolate

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

text: no

characteristic: title


text: Center for Molecular Medicine

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Center for Molecular Medicine

characteristic: Submitter Id

text: house mouse

text: Normal cys

text: Netherlands

text: Mus musculus

text: Mus musculus


text: Center for Molecular Medicine

text: public

characteristic: collection date

text: house mouse

characteristic: common name

text: Low cys

characteristic: description

text: Netherlands

text: Mus musculus

text: Mus musculus


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: public

tag: Namespace:Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, CB10 1SA, UK University of Cambridge, Department of Haematology, Cambridge, CB2 0AW, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 0AW, UK

characteristic: age

text: ArrayExpress

characteristic: broker name

text: not collected

text: human

text: Protocols: Human foetal liver samples were obtained from 3 Ts21 foetuses and 3 healthy foetuses, following termination of pregnancy and informed written consent. The human foetal material was provided by the Joint MRC/Wellcome Trust (Grant MR/R006237/1) Human Developmental Biology Resource (http://www.hdbr.org), with maternal informed consent, in accordance with ethical approval by the National Health Service (NHS) Research Health Authority, REC Ref: 18/LO/0822. HDBR is regulated by the UK Human Tissue Authoriy (HTA; www.hta.gov.uk) and operates in accordance with the relevant HTA Codes of Practice. Foetal livers were received in L15 media and processed within 3h from dissection. Livers were cut in smaller pieces with a scalpel and transferred to a tube containing prewarmed digestion media: RPMI (Gibco) supplemented with 10% FBS (Gibco), penicillin/streptomycin (10U/ml penicillin, 100ng/ml streptomycin, Sigma Aldrich), 2mM L-glutamine (Thermo Scientific), 1x MEM NEAA (Gibco), 1mM Sodium pyruvate (Gibco) and 1.6mg/ml Collagenase-IV (Sigma Aldrich). The tube was vortexed for 10”, then incubated at 37ºC for 30 minutes, and vortexed for 10” every 15 minutes. The digested tissue was filtered through a 100μm filter and diluted in cold D-PBS (Gibco). Cells were centrifuged at 300g for 5 minutes, then aliquoted and cryopreserved in KnockOut Serum Replacement (Gibco) + 5% DMSO (Sigma Aldrich). Cells were stored in liquid nitrogen until further analysis. On the day of FACS sorting, cells were rapidly thawed at 37℃ and transferred to complete RPMI media. Live cell enrichment was performed using MACS Dead Cell Removal Kit following the manufacturer's instructions. For FACS sorting, cells were stained with Zombie Aqua to exclude dead cells and CD45-FITC (MHCD4501) for 30 minutes at 4°C. Cells were centrifuged for 5 minutes at 300 g, 4°C, resuspended in a final volume of 500 μl of 5% FBS in PBS and subsequently filtered into polypropylene FACS tubes. Cells were sorted on a BD FACS Aria Fusion.Nuclei preparation was performed following 10x genomics recommendations. Sorted cells were centrifuged at 300g for 10' at 4ºC. Pellets were resuspended in 45ul chilled lysis buffer (), and incubated on ice for 5'. 50ul of chilled wash buffer () was added and nuclei were centrifuged at 500g for 7' at 4ºC. After removing the supernatant, the nuclear pellet was washed with 45ul chilled diluted nuclei buffer (1x) without pipetting. Nuclei were centrifuged at 500g for 10' at 4ºC. Nuclei were resuspended in 7ul of chilled diluted nuclei buffer and counted. 15,000 nuclei were targeted for library preparation. Immediately after cell enrichment, single nuclei libraries were prepared on the 10x Chromium with a target of 15,000 nuclei. Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Bundle and Chromium Next GEM Chip J Single Cell Kit were used. Immediately after cell enrichment, single nuclei libraries were prepared on the 10x Chromium with a target of 15,000 nuclei. Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Bundle and Chromium Next GEM Chip J Single Cell Kit were used.

text: fetus

characteristic: developmental stage

text: Down syndrome

characteristic: disease

text: not collected

characteristic: individual

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: liver

characteristic: organism part

text: T21 15582 nuclei A

characteristic: original source name

text: ATAC-seq

characteristic: protocol

text: Homo sapiens


characteristic: External Id

text: Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, CB10 1SA, UK University of Cambridge, Department of Haematology, Cambridge, CB2 0AW, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 0AW, UK

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: ArrayExpress

text: not collected

text: human

text: fetus

text: normal

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: liver

text: ATAC-seq

text: Homo sapiens

characteristic: title


characteristic: ENA first public

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: not collected

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: fetus

text: Down syndrome

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: liver

text: T21 15582 nuclei A

text: RNA-Seq

text: Homo sapiens


characteristic: ENA-CHECKLIST

text: public

characteristic: age

text: ArrayExpress

characteristic: broker name

text: not collected

text: human

text: fetus

characteristic: developmental stage

text: Down syndrome

characteristic: disease

text: not collected

characteristic: individual

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: liver

characteristic: organism part

text: T21 15582 nuclei B

text: RNA-Seq

text: Homo sapiens


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: ArrayExpress

text: not collected

text: human

text: fetus

text: Down syndrome

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: liver

characteristic: original source name

text: RNA-Seq

characteristic: protocol

text: Homo sapiens


characteristic: ENA first public

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: not collected

characteristic: collection date

text: human

text: fetus

text: normal

text: not collected

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: liver

text: RNA-Seq

text: Homo sapiens

characteristic: title


characteristic: ENA-CHECKLIST

text: public

characteristic: age

text: ArrayExpress

characteristic: broker name

text: not collected

text: human

characteristic: common name

characteristic: description

text: fetus

characteristic: developmental stage

text: Down syndrome

characteristic: disease

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: liver

text: T21 15582 nuclei B

text: ATAC-seq

text: Homo sapiens


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: ArrayExpress

text: not collected

text: human

text: fetus

text: Down syndrome

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: liver

characteristic: organism part

text: ATAC-seq

text: Homo sapiens


E-MTAB-13091:Sample 1

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13091:Sample 1

tag: Namespace:Sandberg lab, Department of Cell and Molecular Biology, Karolinska Institutet

characteristic: Submitter Id

text: ArrayExpress

text: lymphoblast

text: not collected

characteristic: collection date

text: human

text: Protocols: The experiments used K562 cells from DSMZ (ACC-10) K562 cells were were grown in RPMI 1640 medium supplemented with 10% fetal bovine serum and 1% antibiotics consisting of a Penicillin/ Streptomycin combination at 37°C and 5% CO2. Cells were grown in T25-flasks, seeded to a density of 200,000 cells/ ml and split 1:4 once the density approached 1,000,000 cells/ ml. Cells were treated with compounds from a panel of epigenetically active drugs at a concentration of 12.5 micromolar either one or three hours before harvesting. Additionally, 200 micromolar 4sU was added one hour before cell harvest. RNA was isolated using magnetic beads following the BOMB-bio protocol 8.2 version 1.0 The sequencing libraries were prepared from isolated RNA through mRNA capture with an oligo-dT primer, and full length cDNA molecules were constructed through the use of a template switching oligo. The resulting cDNA fragments were pre-amplified through PCR and then fragmented through tagmentation to yield sequence ready fragments.

text: adult

text: chronic myelogenous leukemia

text: wild type genotype

characteristic: genotype

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 1


E-MTAB-13091:Sample 2

characteristic: ENA-CHECKLIST

text: Sandberg lab, Department of Cell and Molecular Biology, Karolinska Institutet

text: public

text: E-MTAB-13091:Sample 2

text: ArrayExpress

characteristic: broker name

text: lymphoblast

text: not collected

text: human

characteristic: common name

text: Protocols: The experiments used K562 cells from DSMZ (ACC-10) K562 cells were were grown in RPMI 1640 medium supplemented with 10% fetal bovine serum and 1% antibiotics consisting of a Penicillin/ Streptomycin combination at 37°C and 5% CO2. Cells were grown in T25-flasks, seeded to a density of 200,000 cells/ ml and split 1:4 once the density approached 1,000,000 cells/ ml. Cells were treated with the HDAC inhibitor SAHA (vorinostat) at a dose of 10 micromolar for either 30 or 60 minutes. 200 micromolar 4sU was added to the cells at time of treatment. RNA was isolated using magnetic beads following the BOMB-bio protocol 8.2 version 1.0 The sequencing libraries were prepared from isolated RNA through mRNA capture with an oligo-dT primer, and full length cDNA molecules were constructed through the use of a template switching oligo. The resulting cDNA fragments were pre-amplified through PCR and then fragmented through tagmentation to yield sequence ready fragments.

characteristic: description

text: adult

characteristic: developmental stage

text: chronic myelogenous leukemia

text: wild type genotype

text: not collected

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 2

characteristic: title


E-MTAB-13091:Sample 3

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-13091:Sample 3

text: ArrayExpress

text: lymphoblast

text: not collected

text: human

text: Protocols: The experiments used K562 cells from DSMZ (ACC-10) K562 cells were were grown in RPMI 1640 medium supplemented with 10% fetal bovine serum and 1% antibiotics consisting of a Penicillin/ Streptomycin combination at 37°C and 5% CO2. Cells were grown in T25-flasks, seeded to a density of 200,000 cells/ ml and split 1:4 once the density approached 1,000,000 cells/ ml. Cells were treated with the HDAC inhibitor SAHA (vorinostat) at a dose of 10 micromolar for either 6 or 24 hours. No 4sU was used in this experiment. RNA was isolated using magnetic beads following the BOMB-bio protocol 8.2 version 1.0 The sequencing libraries were prepared from isolated RNA through mRNA capture with an oligo-dT primer, and full length cDNA molecules were constructed through the use of a template switching oligo. The resulting cDNA fragments were pre-amplified through PCR and then fragmented through tagmentation to yield sequence ready fragments.

text: adult

text: chronic myelogenous leukemia

characteristic: disease

text: wild type genotype

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 3


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: age

text: ArrayExpress

text: hematopoietic stem cell

text: not collected

text: house mouse

text: Protocols: Long-term HSCs (LT-HSCs sorted from 12-week-old WT and Ppm1DT/+ mice using Influx instrument (BD Biosciences). RNA was isolated from c-Kit+ cells isolated from the genotypes using KAPA mRNA HyperPrep Kit (Kapa Biosystems). Library was prepared using Illumina’s KAPA mRNA Hyperprep Kit as per manufactures instruction

text: adult

text: not collected

text: not applicable

text: Mus musculus

text: Mus musculus

text: bone marrow

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex

characteristic: strain


characteristic: ENA-CHECKLIST

text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: hematopoietic stem cell

text: not collected

characteristic: collection date

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: not collected

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: ArrayExpress

text: hematopoietic stem cell

text: not collected

text: house mouse

text: adult

text: wild type genotype

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: male

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: age

text: ArrayExpress

text: hematopoietic stem cell

text: not collected

text: house mouse

text: adult

text: wild type genotype

text: not collected

text: not applicable

text: Mus musculus

text: Mus musculus

text: bone marrow

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

characteristic: strain


text: public

tag: Namespace:Institute of Chemical Biology, National Hellenic Research Foundation

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: human

characteristic: common name

text: Protocols: Colo-205 and RKO cells were were obtained from the American Type Culture Collection (ATCC) and grown in DMEM medium supplemented with 10% Fetal Bovine Serum and 1% penicillin/streptomycin and amino acids (all from Thermo Fisher Scientific, Waltham, MA, USA) at 37°C, 5% CO2. Cells were seeded in 6-well plates and left to attach overnight. Treatment with the indicated compounds was performed for 24h. RNA was extracted from cells using the TRIzol Reagent (Invitrogen) followed by a clean-up step combined with on-column DNase digestion using the RNeasy kit (Qiagen) according to manufacturers' instructions. After the total RNA extraction and DNase I treatment, magnetic beads with Oligo (dT) are used to isolate mRNA. Then cDNA is synthesized using the mRNA fragments as templates. Short fragments are purified and resolved with EB buffer for end reparation and single nucleotide A (adenine) addition. After that, the short fragments are connected with adapters. The suitable fragments are selected for the PCR amplification as templates.

characteristic: description

text: colorectal adenoma

characteristic: disease

text: wild type genotype

characteristic: genotype

text: not collected

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: colorectum

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Institute of Chemical Biology, National Hellenic Research Foundation

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: ArrayExpress

text: not collected

text: human

text: colorectal adenoma

text: wild type genotype

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: colorectum

text: Homo sapiens

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: RKO

text: not collected

text: human

text: colorectal adenoma

text: wild type genotype

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: colorectum

characteristic: organism part

text: Homo sapiens


text: Biozentrum, University of Basel

text: public

tag: Namespace:Biozentrum, University of Basel

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: human

characteristic: common name

text: Protocols: Cells were propagated to 70-90% confluence in T175cm2 flasks, incubated with cycloheximide (100 µg/ml, C7698, Sigma) 30min prior to harvest (scraping), washed (2x) with cold PBS buffer and resuspended in 400µl of 1x lysis buffer (#9803, CST) supplemented with protease/phosphatase inhibitor cocktail (#78442, Thermo Fisher) and 5mM of MgCl2 (HN03.1, ROTH). Cells were mechanically disrupted by vortexing (3x 10sec at max. output, 10min on ice in between), cleared by centrifugation (10min, 21.000rcf, 4°C) Hot phenol extraction of RNA TruSeq RNA Library Prep Kit v2 Illumina

characteristic: description

text: adult

characteristic: developmental stage

text: breast carcinoma

text: wild type genotype

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Biozentrum, University of Basel

text: public

text: ArrayExpress

text: not collected

text: human

text: adult

text: breast carcinoma

characteristic: disease

text: sox2 full length overexpression

characteristic: genotype

text: not collected

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: mammary gland

text: Homo sapiens


characteristic: ENA-CHECKLIST

text: Biozentrum, University of Basel

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: ArrayExpress

text: not collected

text: human

text: adult

text: breast carcinoma

text: sox2 NesC terminal overexpression

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

characteristic: title


text: Biozentrum, University of Basel

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: not collected

text: human

text: adult

text: breast carcinoma

text: sox2 Nter overexpression

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

characteristic: organism part

text: Homo sapiens


text: Plasmodium falciparum

characteristic: ArrayExpress-SPECIES

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: not provided

characteristic: collection date

text: malaria parasite P. falciparum

characteristic: common name

text: not provided

text: Plasmodium falciparum

text: Plasmodium falciparum


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

characteristic: age

text: ArrayExpress

text: not collected

text: human

text: Protocols: Human foetal bone and liver samples were obtained from 15 Ts21 foetuses aged 12-20 pcw and 5 healthy foetuses aged 11-19 pcw, following termination of pregnancy and informed written consent. The human foetal material was provided by the Joint MRC/Wellcome Trust (Grant MR/R006237/1) Human Developmental Biology Resource (http://www.hdbr.org), with maternal informed consent, in accordance with ethical approval by the National Health Service (NHS) Research Health Authority, REC Ref: 18/LO/0822. HDBR is regulated by the UK Human Tissue Authoriy (HTA; www.hta.gov.uk) and operates in accordance with the relevant HTA Codes of Practice. Foetal livers and femurs were received in L15 media and processed within 3h from dissection. Livers were cut in smaller pieces with a scalpel and transferred to a tube containing prewarmed digestion media: RPMI (Gibco) supplemented with 10% FBS (Gibco), penicillin/streptomycin (10U/ml penicillin, 100ng/ml streptomycin, Sigma Aldrich), 2mM L-glutamine (Thermo Scientific), 1x MEM NEAA (Gibco), 1mM Sodium pyruvate (Gibco) and 1.6mg/ml Collagenase-IV (Sigma Aldrich). The tube was vortexed for 10”, then incubated at 37ºC for 30 minutes, and vortexed for 10” every 15 minutes. The digested tissue was filtered through a 100μm filter and diluted in cold D-PBS (Gibco). Cells were centrifuged at 300g for 5 minutes, then aliquoted and cryopreserved in KnockOut Serum Replacement (Gibco) + 5% DMSO (Sigma Aldrich). For femurs, adherent material was removed, then the epiphyses were removed with a scalpel and the bone marrow flushed with D-PBS. The remaining bone was cut in small pieces, then ground with a mortar and pestle using digestion media and incubated at 37ºC for 30 minutes, vortexing every 15 minutes. The digested material and the bone marrow flush were mixed and filtered through a 100μm filter. Cells were centrifuged at 300g for 5 minutes, then aliquoted and cryopreserved in KnockOut Serum Replacement (Gibco) + 5% DMSO (Sigma Aldrich). Cells were stored in liquid nitrogen until further analysis.On the day of FACS sorting, cells were rapidly thawed at 37℃ and transferred to complete RPMI media. Live cell enrichment was performed using MACS Dead Cell Removal Kit following the manufacturer's instructions. When depleting for CD235a+ cells, a magnetic negative selection was performed using CD235a Microbeads and MACS LS columns following the manufacturer's instructions.For FACS sorting, cells were stained with Zombie Aqua to exclude dead cells and CD45-FITC (MHCD4501), CD3-AF700 (OKT3), CD19-AF700 (HIB19), CD56-AF700 (BI59), CD8-AF700 (SKI), CD11b-AF700 (CBRM1/5), CD14-AF700 (61D3), CD71-APC (CY1G4), CD235a-APC (GA-RD)for 30 minutes at 4°C. Cells were centrifuged for 5 minutes at 300 g, 4°C, resuspended in a final volume of 500 μl of 5% FBS in PBS and subsequently filtered into polypropylene FACS tubes. Cells were sorted on a BD FACS Aria Fusion. Immediately after cell enrichment, single cell libraries were prepared on the 10x Chromium with a target of 50,000 cells. Chromium Next GEM Single Cell 3' Kit v3.1, Chromium Next GEM Chip G Single Cell Kit and Dual Index Kit TT Set A were used. Immediately after cell enrichment, single cell libraries were prepared on the 10x Chromium with a target of 50,000 cells. Chromium Next GEM Single Cell 3' Kit v3.1, Chromium Next GEM Chip G Single Cell Kit and Dual Index Kit TT Set A were used.

characteristic: description

text: embryo stage

characteristic: developmental stage

text: normal

characteristic: disease

text: not collected

characteristic: immunophenotype

characteristic: individual

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: liver

text: Homo sapiens


characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: not collected

text: human

text: embryo stage

text: normal

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: femur

text: Homo sapiens

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

text: ArrayExpress

text: not collected

characteristic: collection date

text: human

characteristic: common name

text: embryo stage

text: trisomy 21

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: liver

characteristic: organism part

text: Homo sapiens


text: public

characteristic: age

text: ArrayExpress

text: not collected

text: human

characteristic: description

text: embryo stage

characteristic: developmental stage

text: trisomy 21

characteristic: disease

text: not collected

characteristic: immunophenotype

characteristic: individual

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: femur

text: Homo sapiens


characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: not collected

text: human

text: embryo stage

text: trisomy 21

text: not collected

text: CD45+ (+) CD45-/CD235a-/CD71-

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: liver

text: Homo sapiens


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

tag: Namespace:Council for Agricultural Research and Economics (CREA)

text: dicoccon

text: Armenia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: doi

characteristic: isolation and growth condition

characteristic: number of replicons

text: Triticum turgidum

text: seedling

characteristic: plant developmental stage

text: soil

characteristic: plant growth medium

text: leaf

characteristic: plant structure

text: Triticum turgidum

text: landrace

characteristic: subspecific genetic lineage rank

characteristic: title


characteristic: External Id

text: UNIVERSITE DE LIMOGES

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:UNIVERSITE DE LIMOGES

characteristic: collection date

text: house mouse

characteristic: common name

text: Loop analysis Smu Bait in WT1 R

characteristic: description

text: France

text: Mus musculus

text: Mus musculus


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITE DE LIMOGES

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: house mouse

text: Loop analysis Smu Bait in WT2 R

text: France

characteristic: geographic location (country and/or sea)

text: Mus musculus

characteristic: organism

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis Smu Bait in WT3 R

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis Smu Bait in WT4 R

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis Smu Bait in WT5 R

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis Smu Bait in WT1 LPS

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis Smu Bait in WT2 LPS

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis Smu Bait in WT3 LPS

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis Smu Bait in WT4 LPS

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis Smu Bait in 3RRKO1 R

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis Smu Bait in 3RRKO2 R

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis Smu Bait in 3RRKO3 R

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis Smu Bait in 3RRKO1 LPS

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis Smu Bait in 3RRKO2 LPS

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis Smu Bait in 3RRKO3 LPS

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis Smu Bait in c3RR1 R

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis Smu Bait in c3RR2 R

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis Smu Bait in c3RR1 LPS

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis Smu Bait in c3RR2 LPS

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis Smu Bait in c3RR3 LPS

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis CBE Bait in WT1 R

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis CBE Bait in WT2 R

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis CBE Bait in WT1 LPS

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis CBE Bait in WT2 LPS

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis CBE Bait in WT3 LPS

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis CBE Bait in 3RRKO1 R

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis CBE Bait in 3RRKO2 R

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis CBE Bait in 3RRKO1 LPS

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis CBE Bait in 3RRKO2 LPS

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis CBE Bait in c3RR1 R

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis CBE Bait in c3RR2 R

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis CBE Bait in c3RR1 LPS

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis CBE Bait in c3RR2 LPS

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis 3RR Bait in WT1 R

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis 3RR Bait in WT2 R

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis 3RR Bait in WT3 R

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis 3RR Bait in 3RRKO1 R

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis 3RR Bait in 3RRKO2 R

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis 3RR Bait in 3RRKO3 R

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis 3RR Bait in hs567KO1 R

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis 3RR Bait in hs567KO2 R

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis 3RR Bait in hs567KO3 R

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis 3RR Bait in WT1 LPS

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis 3RR Bait in WT2 LPS

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis 3RR Bait in WT3 LPS

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis 3RR Bait in 3RRKO1 LPS

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis 3RR Bait in 3RRKO2 LPS

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis 3RR Bait in hs567KO1 LPS

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Loop analysis 3RR Bait in hs567KO2 LPS

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: CSR Analysis in WT1 LPS

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: CSR Analysis in WT2 LPS

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: CSR Analysis in WT3 LPS

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: CSR Analysis in WT4 LPS

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: CSR Analysis in WT5 LPS

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: CSR Analysis in 3RRKO1 LPS

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: CSR Analysis in 3RRKO2 LPS

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: CSR Analysis in 3RRKO3 LPS

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: CSR Analysis in c3RR1 LPS

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: CSR Analysis in c3RR2 LPS

text: France

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: CSR Analysis in c3RR3 LPS

text: France

text: Mus musculus

text: Mus musculus


Digitalis purpurea plant1

text: public

text: Digitalis purpurea plant1

tag: Namespace:Institute of Plant Biology, TU Braunschweig

text: common foxglove

text: DNA extracted from Digitalis purpurea

text: Germany

text: Digitalis purpurea

text: Digitalis purpurea

text: Digitalis purpurea plant1


Digitalis purpurea plant2

text: Institute of Plant Biology, TU Braunschweig

text: public

text: Digitalis purpurea plant2

text: common foxglove

text: DNA extracted from Digitalis purpurea

text: Germany

text: Digitalis purpurea

text: Digitalis purpurea

text: Digitalis purpurea plant2


Digitalis purpurea plant3

text: public

text: Digitalis purpurea plant3

text: common foxglove

text: DNA extracted from Digitalis purpurea

text: Germany

text: Digitalis purpurea

text: Digitalis purpurea

text: Digitalis purpurea plant3


Digitalis purpurea plant4

text: public

text: Digitalis purpurea plant4

text: common foxglove

text: DNA extracted from Digitalis purpurea

text: Germany

text: Digitalis purpurea

text: Digitalis purpurea

text: Digitalis purpurea plant4


E-MTAB-13105:Sample 1

text: LabMedMolGe

text: public

text: E-MTAB-13105:Sample 1

unit: month

characteristic: age

text: ArrayExpress

characteristic: broker name

text: not collected

text: house mouse

text: Protocols: LoxP (L83) site and a FNFL (Frt- Neo- Frt-LoxP) cassette were engineered to flank exon 2/3 (2 Kb) of the praja2 allele to generate �floxed/neo� praja2 on a Bacterial Artificial Chromosome (BAC). A gene targeting vector was constructed by retrieving the 2 kb short homology arm (5� to L83), the floxed sequence containing exon 2/3, the FNL cassette, and the 5 kb long homology arm (end of FNFL to 3�) into a plasmid vector carrying a DTA (Diphtheria Toxin Alpha chain) negative selection marker. The FNFL cassette confers G418 resistance during gene targeting in KV1 (129B6 hybrid) ES cells and targeted ES cells were confirmed by PCR and southern blotting analysis. One of the ES positive clones was injected into C57BL/6J blastocysts to generate chimeric mice. Chimeric founder male mice were bred with C57BL/6J females for germline transmission and the consequent generation of a mouse line carrying the genetic locus of praja2 under the control of loxP sites. (Authorization number: prot. 2014/0015838). After the deletion of Neo Cassette by Flpe recombinase, praja2 floxed mice were crossed with Hprt-Cre mice for the excision of exon 1 and 2. The resulting heterozygous praja2 KO mice (praja2+/-) were backcrossed into the C57BL/6 J background for three generations (F3). Praja2+/- mice were then interbred to produce homozygous praja2-/- animals. Genomic DNA was extracted as described previously[73] by ear clip biopsies and KO mice were genotyped by PCR using the following primers: FNFFw, 5�- GATGTTCCAAAGGAGAACACCTCAG-3�; FNFRw, 5�-TATGCAGGAAAAACACACTC GGTTC-3�; LNLFw, 5�-GAAAGGGTTGGTTTGGAGTACAGGT-3�. The wild type fragment (466 bp) was generated by the primers FNFFw and FNFRw; the KO fragment (320 bp) was generated by the primers LNLFw and FNFRw. Amplifcation conditions were: 95 �C for 5 min; 35 cycles: 95 �C for 30 s, 62 �C for 30 s, 72 �C for 1 min; 72 �C for 10 min. Animals were group housed (five per cage), at a constant temperature (22�1 �C) on a 12 h light/dark cycle (lights on at 7 AM) with food and water ad libitum. (Autorization n. 924/2017-PR del 29/11/2017) RNAs were extracted from FFPE-embedded tissues using RNeasy FFPE kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. RNA purity was assessed by NanoDrop� 2000/2000c spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA), whereas its concentration and integrity were measured by Qubit fluorimeter using RNA assay kit (both Life Technologies, Carlsbad, CA, USA) and Agilent 4200 Tapestation System (Agilent, Santa Clara, CA, USA), respectively. Indexed libraries were prepared starting from 500 ng of total RNA using TruSeq Stranded Total RNA preparation kit (Illumina Inc., San Diego, CA, USA).

text: adult

characteristic: developmental stage

text: renal carcinoma

characteristic: disease

characteristic: genotype

text: not collected

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: kidney

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex

text: C57BL/6 J

characteristic: strain

text: Sample 1


E-MTAB-13105:Sample 2

characteristic: External Id

text: LabMedMolGe

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13105:Sample 2

text: ArrayExpress

text: not collected

characteristic: collection date

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: renal carcinoma

text: not collected

text: not applicable

text: Mus musculus

text: Mus musculus

text: kidney

text: Mus musculus

text: male

text: C57BL/6 J

text: Sample 2

characteristic: title


E-MTAB-13105:Sample 3

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: LabMedMolGe

characteristic: INSDC first public

text: public

text: E-MTAB-13105:Sample 3

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: not collected

text: house mouse

text: adult

text: renal carcinoma

text: wild type genotype

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: kidney

text: Mus musculus

text: male

text: C57BL/6 J

text: Sample 3


E-MTAB-13105:Sample 4

characteristic: External Id

text: LabMedMolGe

characteristic: INSDC center name

text: public

text: E-MTAB-13105:Sample 4

text: ArrayExpress

text: not collected

text: house mouse

text: adult

characteristic: developmental stage

text: renal carcinoma

characteristic: disease

text: wild type genotype

characteristic: genotype

text: not collected

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: kidney

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex

text: C57BL/6 J

characteristic: strain

text: Sample 4


E-MTAB-13105:Sample 5

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: LabMedMolGe

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13105:Sample 5

text: ArrayExpress

text: not collected

characteristic: collection date

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: renal carcinoma

text: wild type genotype

text: not collected

text: not applicable

text: Mus musculus

text: Mus musculus

text: kidney

text: Mus musculus

text: male

text: C57BL/6 J

text: Sample 5

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

tag: Namespace:Institute of Food, Nutrition and Health, ETH Zurich

characteristic: Submitter Id

text: human

text: Treatment reactor

text: Switzerland

characteristic: geographic location (country and/or sea)

text: Homo sapiens

characteristic: organism

text: Homo sapiens


tag: attribute

text: Institute of Food, Nutrition and Health, ETH Zurich

text: public

text: human

text: Treatment reactor

text: Switzerland

text: Homo sapiens

text: Homo sapiens


text: public

text: human

text: Inoculum reactor

text: Switzerland

text: Homo sapiens

text: Homo sapiens


text: public

text: human

text: Feces

text: Switzerland

text: Homo sapiens

text: Homo sapiens


text: Plasmodium falciparum

characteristic: ArrayExpress-SPECIES

text: SC

text: public

text: not provided

text: malaria parasite P. falciparum

text: not provided

text: Plasmodium falciparum

text: Plasmodium falciparum


characteristic: ENA-CHECKLIST

text: public

tag: Namespace:Leibniz Institute of Freshwater Ecology and Inland Fisheries

text: Estuarine

characteristic: broad-scale environmental context

characteristic: depth

text: Site 1 High rep a tech 2

characteristic: elevation

text: soil

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: estuarine marsh

characteristic: local environmental context

text: estuary metagenome

text: Metagenomes from Elbe Estuary Particles (2021-2022)

characteristic: project name

text: estuary metagenome

text: NovaSeq 6000 S4

characteristic: sequencing method

text: soil

characteristic: soil environmental package


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Leibniz Institute of Freshwater Ecology and Inland Fisheries

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Estuarine

characteristic: collection date

text: Site 1 High rep c tech 2

characteristic: description

text: soil

text: Germany

text: metagenome

text: estuarine marsh

text: estuary metagenome

text: estuary metagenome

text: NovaSeq 6000 S4

text: soil

characteristic: title


characteristic: ENA-CHECKLIST

text: public

characteristic: Submitter Id

text: Estuarine

characteristic: broad-scale environmental context

characteristic: depth

text: Site 1 Low rep a tech 2

characteristic: elevation

text: soil

characteristic: environmental medium

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

text: estuarine marsh

text: estuary metagenome

characteristic: organism

text: estuary metagenome

text: NovaSeq 6000 S4

text: soil


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Estuarine

text: Site 1 Low rep c tech 2

text: soil

text: Germany

text: metagenome

characteristic: investigation type

text: estuarine marsh

characteristic: local environmental context

text: estuary metagenome

characteristic: project name

text: estuary metagenome

text: NovaSeq 6000 S4

characteristic: sequencing method

text: soil

characteristic: soil environmental package


characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Estuarine

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

text: Site 1 P zone rep a tech 2

characteristic: description

text: soil

text: Germany

text: metagenome

text: estuarine marsh

text: estuary metagenome

text: estuary metagenome

text: NovaSeq 6000 S4

text: soil

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: Estuarine

text: Site 1 p zone rep c tech 2

characteristic: elevation

text: soil

characteristic: environmental medium

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

text: estuarine marsh

text: estuary metagenome

characteristic: organism

text: estuary metagenome

text: NovaSeq 6000 S4

text: soil


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Estuarine

text: Site 2 High rep a tech 2

text: soil

text: Germany

text: metagenome

characteristic: investigation type

text: estuarine marsh

characteristic: local environmental context

text: estuary metagenome

characteristic: project name

text: estuary metagenome

text: NovaSeq 6000 S4

characteristic: sequencing method

text: soil

characteristic: soil environmental package


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: Submitter Id

text: Estuarine

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

text: Site 2 High rep c tech 2

characteristic: description

text: soil

text: Germany

text: metagenome

text: estuarine marsh

text: estuary metagenome

text: estuary metagenome

text: NovaSeq 6000 S4

text: soil

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Estuarine

text: Site 2 Low rep a tech 2

characteristic: elevation

text: soil

characteristic: environmental medium

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

text: estuarine marsh

text: estuary metagenome

characteristic: organism

text: estuary metagenome

text: NovaSeq 6000 S4

text: soil


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: SRA accession

text: Estuarine

text: Site 2 Low rep c tech 2

text: soil

text: Germany

text: metagenome

characteristic: investigation type

text: estuarine marsh

characteristic: local environmental context

text: estuary metagenome

characteristic: project name

text: estuary metagenome

text: NovaSeq 6000 S4

characteristic: sequencing method

text: soil

characteristic: soil environmental package


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Submitter Id

text: Estuarine

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

text: Site 2 P zone rep a tech 2

characteristic: description

text: soil

text: Germany

text: metagenome

text: estuarine marsh

text: estuary metagenome

text: estuary metagenome

text: NovaSeq 6000 S4

text: soil

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: Estuarine

text: Site 2 p zone rep c tech 2

characteristic: elevation

text: soil

characteristic: environmental medium

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

text: estuarine marsh

text: estuary metagenome

characteristic: organism

text: estuary metagenome

text: NovaSeq 6000 S4

text: soil


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Estuarine

text: Site 3 High rep a tech 2

text: soil

text: Germany

text: metagenome

characteristic: investigation type

text: estuarine marsh

characteristic: local environmental context

text: estuary metagenome

characteristic: project name

text: estuary metagenome

text: NovaSeq 6000 S4

characteristic: sequencing method

text: soil

characteristic: soil environmental package


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: Submitter Id

text: Estuarine

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

text: Site 3 Low rep a tech 2

characteristic: description

text: soil

text: Germany

text: metagenome

text: estuarine marsh

text: estuary metagenome

text: estuary metagenome

text: NovaSeq 6000 S4

text: soil

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Estuarine

text: Site 3 Low rep c tech 2

characteristic: elevation

text: soil

characteristic: environmental medium

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

text: estuarine marsh

text: estuary metagenome

characteristic: organism

text: estuary metagenome

text: NovaSeq 6000 S4

text: soil


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: SRA accession

text: Estuarine

text: Site 3 P zone rep a tech 2

text: soil

text: Germany

text: metagenome

characteristic: investigation type

text: estuarine marsh

characteristic: local environmental context

text: estuary metagenome

characteristic: project name

text: estuary metagenome

text: NovaSeq 6000 S4

characteristic: sequencing method

text: soil

characteristic: soil environmental package


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Submitter Id

text: Estuarine

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

text: Site 3 p zone rep c tech 2

characteristic: description

text: soil

text: Germany

text: metagenome

text: estuarine marsh

text: estuary metagenome

text: estuary metagenome

text: NovaSeq 6000 S4

text: soil

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

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