text: KAUST, CDA, Simon Krattinger's group

text: public

text: ena-SAMPLE-TAB-10-04-2022-12:34:50:883-16140

text: Triticum monococcum

text: Triticum monococcum


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: KAUST, CDA, Simon Krattinger's group

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-10-04-2022-12:34:50:883-16141

tag: Namespace:KAUST, CDA, Simon Krattinger's group

characteristic: Submitter Id

text: Triticum monococcum

characteristic: organism

characteristic: sample alias

text: Triticum monococcum

characteristic: title


text: KAUST, CDA, Simon Krattinger's group

text: public

text: ena-SAMPLE-TAB-10-04-2022-12:34:50:883-16142

text: Triticum monococcum

text: Triticum monococcum


text: KAUST, CDA, Simon Krattinger's group

text: public

text: ena-SAMPLE-TAB-10-04-2022-12:57:38:221-19788

text: Triticum monococcum

text: Triticum monococcum


text: KAUST, CDA, Simon Krattinger's group

text: public

text: ena-SAMPLE-TAB-10-04-2022-12:57:38:221-19789

text: Triticum monococcum

text: Triticum monococcum


text: KAUST, CDA, Simon Krattinger's group

text: public

text: ena-SAMPLE-TAB-10-04-2022-12:57:38:221-19790

text: Triticum monococcum

text: Triticum monococcum


text: KAUST, CDA, Simon Krattinger's group

text: public

text: ena-SAMPLE-TAB-10-04-2022-12:57:38:221-19791

text: Triticum monococcum

text: Triticum monococcum


text: KAUST, CDA, Simon Krattinger's group

text: public

text: ena-SAMPLE-TAB-10-04-2022-12:57:38:221-19792

text: Triticum monococcum

text: Triticum monococcum


text: KAUST, CDA, Simon Krattinger's group

text: public

text: ena-SAMPLE-TAB-10-04-2022-12:57:38:221-19793

text: Triticum monococcum

text: Triticum monococcum


text: KAUST, CDA, Simon Krattinger's group

text: public

text: ena-SAMPLE-TAB-10-04-2022-12:57:38:221-19794

text: Triticum monococcum

text: Triticum monococcum


text: KAUST, CDA, Simon Krattinger's group

text: public

text: ena-SAMPLE-TAB-10-04-2022-12:57:38:221-19795

text: Triticum monococcum

text: Triticum monococcum


text: KAUST, CDA, Simon Krattinger's group

text: public

text: ena-SAMPLE-TAB-10-04-2022-12:57:38:221-19796

text: Triticum monococcum

text: Triticum monococcum


text: KAUST, CDA, Simon Krattinger's group

text: public

text: ena-SAMPLE-TAB-10-04-2022-12:57:38:221-19797

text: Triticum monococcum

text: Triticum monococcum


text: KAUST, CDA, Simon Krattinger's group

text: public

text: ena-SAMPLE-TAB-10-04-2022-12:57:38:221-19798

text: Triticum monococcum

text: Triticum monococcum


text: KAUST, CDA, Simon Krattinger's group

text: public

text: ena-SAMPLE-TAB-10-04-2022-12:57:38:221-19799

text: Triticum monococcum

text: Triticum monococcum


text: KAUST, CDA, Simon Krattinger's group

text: public

text: ena-SAMPLE-TAB-10-04-2022-12:57:38:221-19800

text: Triticum monococcum

text: Triticum monococcum


text: KAUST, CDA, Simon Krattinger's group

text: public

text: ena-SAMPLE-TAB-10-04-2022-12:57:38:221-19801

text: Triticum monococcum

text: Triticum monococcum


text: KAUST, CDA, Simon Krattinger's group

text: public

text: ena-SAMPLE-TAB-10-04-2022-12:57:38:221-19802

text: Triticum monococcum

text: Triticum monococcum


text: KAUST, CDA, Simon Krattinger's group

text: public

text: ena-SAMPLE-TAB-10-04-2022-12:57:38:221-19803

text: Triticum monococcum

text: Triticum monococcum


text: Oesophageal Epithelium

characteristic: ArrayExpress-PHENOTYPE

text: unknown

characteristic: ArrayExpress-SEX

text: Mus musculus

characteristic: ArrayExpress-SPECIES

text: Unknown

characteristic: GENDER

text: SC

text: public

text: Oesophageal Epithelium

characteristic: PHENOTYPE

text: house mouse

characteristic: common name

text: Mus musculus

text: Mus musculus


text: Skin

text: unknown

text: Mus musculus

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Unknown

text: SC

text: public

text: Skin

text: house mouse

text: Mus musculus

text: Mus musculus


characteristic: External Id

text: UNIVERSITY OF AMSTERDAM

characteristic: INSDC center name

characteristic: INSDC first public

text: public

tag: Namespace:UNIVERSITY OF AMSTERDAM

unit: g

characteristic: amount or size of sample collected

text: Rodrigo Therezan

text: tomato

text: Stem trichome mRNA-seq data on MT-Pic related genotypes

characteristic: description

text: wild-type (MicroTom cultivar)

characteristic: genotype

text: Netherlands

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: North Holland, Amsterdam

characteristic: geographic location (region and locality)

text: glasshouse

characteristic: growth facility

characteristic: isolation and growth condition

text: Solanum lycopersicum

characteristic: plant developmental stage

text: soil

characteristic: plant growth medium

characteristic: plant structure

text: diploid

characteristic: ploidy

text: manual collection of two leaflets of the fourth fully developed leaf, in 50ml falcon tubes

characteristic: sample collection device or method

text: healthy

characteristic: sample health state

text: frozen in liquid N2 and homogenized into frozen-powder by vortexing

characteristic: sample material processing

text: 4 weeks

characteristic: sampled age

text: Solanum lycopersicum


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF AMSTERDAM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: Rodrigo Therezan

text: tomato

characteristic: common name

text: Near Isogenic Line (MicroTom-Pic)

text: Netherlands

text: North Holland, Amsterdam

text: glasshouse

text: Solanum lycopersicum

characteristic: organism

text: soil

text: diploid

text: healthy

text: 4 weeks

text: Solanum lycopersicum

characteristic: title


text: UNIVERSITY OF AMSTERDAM

text: public

text: Rodrigo Therezan

characteristic: description

text: wild-type (S. habrochaites PI127826)

characteristic: genotype

text: Netherlands

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: North Holland, Amsterdam

characteristic: geographic location (region and locality)

text: glasshouse

characteristic: growth facility

characteristic: isolation and growth condition

text: Solanum habrochaites

characteristic: plant developmental stage

text: soil

characteristic: plant growth medium

characteristic: plant structure

text: diploid

characteristic: ploidy

characteristic: sample collection device or method

text: healthy

characteristic: sample health state

text: 4 weeks

text: Solanum habrochaites


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Max Planck Institute of Immunobiology and Epigenetics (MPI-IE)

characteristic: Submitter Id

unit: week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: LSK GFP+

text: house mouse

characteristic: common name

text: Protocols: 1000 GFP+ LSK cells using the following antibodies: anti-lineage [anti-CD4 (clone GK1.5), anti-CD8a (53-6.7), anti-CD11b (M1/70), anti- B220 (RA3-6B2), anti-GR1 (RB6-8C5) and anti-TER119 (Ter-119)] all PE-Cy7; anti-CD117/c-Kit (2B8)-PE; anti-Ly6a/Sca-1 (D7)-APCCy7. Sorted cells were collected into ice-cold RNA lysis buffer (ARCTURUS PicoPure RNA Isolation Kit (Life Technologies, Invitrogen)) and stored at -80C until further usage. Total RNA isolation was using ARCTURUS PicoPure RNA Isolation Kit (Life Technologies, Invitrogen) according to the manufacturer’s instructions. DNAse treatment was performed using RNAse-free DNAse Set (Qiagen). Total RNA was used as starting material for cDNA-libraries preparation using the SMARTseq v4 Ultra Low RNA Kit (Takara) according to the manufacturers indications.DNAse treatment was performed using RNAse-free DNAse Set (Qiagen). Total RNA was used as starting material for cDNA-libraries preparation using the SMARTseq v4 Ultra Low RNA Kit (Takara) according to the manufacturers indications. Of note, 12 cycles were used for the amplification of cDNA. Sequencing libraries were prepared from 10ng of cDNA libraries as input using NEBNext Ultra II FS DNA library prep kit (#E7805S, NEB) according to manufacturer's protocol together with unique dual indexing barcodes (NEB, #E6440). Libraries were amplified with 7 PCR-cycles before Ampure bead (Beckman-Coultier) clean-up and subsequent quality control.

text: adult early hematopoiesis

characteristic: developmental stage

text: empty vector

text: mouse 1

characteristic: individual

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: bone marrow

characteristic: organism part

text: MLL-AF9 retroviral

characteristic: phenotype

text: Mus musculus

text: female

characteristic: sex

text: fresh specimen

characteristic: specimen with known storage state

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Max Planck Institute of Immunobiology and Epigenetics (MPI-IE)

characteristic: INSDC center name

text: public

text: ArrayExpress

text: LSK GFP+

text: house mouse

characteristic: description

text: adult early hematopoiesis

text: empty vector

characteristic: genotype

text: mouse 2

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

text: MLL-AF9 retroviral

text: Mus musculus

text: female

text: fresh specimen

characteristic: title


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: LSK GFP+

text: house mouse

characteristic: common name

text: adult early hematopoiesis

characteristic: developmental stage

text: empty vector

text: mouse 3

text: not applicable

text: Mus musculus

text: Mus musculus

text: bone marrow

text: MLL-AF9 retroviral

text: Mus musculus

text: female

text: fresh specimen


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: ArrayExpress

text: LSK GFP+

text: house mouse

text: adult early hematopoiesis

text: Gprc5c overexpression

text: mouse 1

characteristic: individual

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: bone marrow

characteristic: organism part

text: MLL-AF9 retroviral

characteristic: phenotype

text: Mus musculus

text: female

characteristic: sex

text: fresh specimen

characteristic: specimen with known storage state

characteristic: strain


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: LSK GFP+

text: house mouse

characteristic: description

text: adult early hematopoiesis

text: Gprc5c overexpression

characteristic: genotype

text: mouse 2

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

text: MLL-AF9 retroviral

text: Mus musculus

text: female

text: fresh specimen

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: LSK GFP+

text: house mouse

characteristic: common name

text: adult early hematopoiesis

characteristic: developmental stage

text: Gprc5c overexpression

text: mouse 3

text: not applicable

text: Mus musculus

text: Mus musculus

text: bone marrow

text: MLL-AF9 retroviral

text: Mus musculus

text: female

text: fresh specimen


text: Schistosoma mansoni

characteristic: ArrayExpress-SPECIES

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: Schistosoma mansoni

text: Schistosoma mansoni

text: Schistosoma mansoni


E-MTAB-11677:Sample 1

text: Bioinformatician

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11677:Sample 1

text: ArrayExpress

text: marginal zone B cell

text: house mouse

text: Protocols: C57BL6 adult mouse sorted Marginal Bcells in spleen at 16-wk old. Euthanized with CO2 and then opened abdomen to get spleen. Spleen then smashed and filtered after obtaining a suspension with isolated spleen cells. Then B cells were pre-enriched from the suspension using Stem Cell B cell isolation kit. Cells were stained for B220, CD21 and CD23 and sorted in Aria III sorter (Cd23low Cd21hi population). 16wks C57BL6 mouse RNA was isolated from sorted MZB Micro RNA Plus (Quiagen) from reconstituted lethally irradiated LDLr-/-: Rag2-/- (mice that do not have T and B cells) with a BM containing 100% CD4Cre/+; Bcl6flox/flox (No Tfh) or a mixed chimaera containing 80% CD4Cre/+; Bcl6flox/flox + 20% CD4+/+; Bcl6flox/flox (WT). Align to GRCm39 reference genome using star (version 2.6.1d) RNA was isolated from sorted MZB Micro RNA Plus (Quiagen) from reconstituted lethally irradiated LDLr-/-: Rag2-/- (mice that do not have T and B cells) with a BM containing 100% CD4Cre/+; Bcl6flox/flox (No Tfh) or a mixed chimaera containing 80% CD4Cre/+; Bcl6flox/flox + 20% CD4+/+; Bcl6flox/flox (WT). The quality of the RNA extracted was checked using a Bioanalyser RNA Pico kit (Agilent) with RIN values > 7. RNA (2.5 ng) was whole transcriptome-amplified using the Ovation RNA-seq System V2 (NuGEN). Prepare the RNA-seq library, the amplified cDNA (2μg per sample) was fragmented to 200 bp using a Bioruptor Sonicator (Diagenode), and barcode ligation and end repair were performed using the Ovation Rapid DR Library System (NuGEN).

text: adult

text: normal

characteristic: disease

text: LDLr-/-: Rag2-/-

text: lethally irradiated then transfected with bone marrow from CD4Cre/+; Bcl6flox/flox mice

characteristic: growth condition

characteristic: individual

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: spleen

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex

characteristic: strain

text: Sample 1


E-MTAB-11677:Sample 10

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Bioinformatician

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11677:Sample 10

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: marginal zone B cell

text: house mouse

characteristic: description

text: adult

text: normal

text: 80% CD4Cre/+; Bcl6flox/flox + 20% CD4+/+; Bcl6flox/flox (WT)

characteristic: genotype

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spleen

text: Mus musculus

text: female

text: Sample 10

characteristic: title


E-MTAB-11677:Sample 11

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Bioinformatician

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11677:Sample 11

text: ArrayExpress

characteristic: broker name

text: marginal zone B cell

text: house mouse

characteristic: common name

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: not applicable

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: female

text: Sample 11


E-MTAB-11677:Sample 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Bioinformatician

text: public

characteristic: INSDC status

text: E-MTAB-11677:Sample 2

text: ArrayExpress

text: marginal zone B cell

text: house mouse

text: adult

text: normal

text: LDLr-/-: Rag2-/-

characteristic: growth condition

characteristic: individual

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: spleen

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex

characteristic: strain

text: Sample 2


E-MTAB-11677:Sample 3

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Bioinformatician

text: public

characteristic: SRA accession

text: E-MTAB-11677:Sample 3

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: marginal zone B cell

text: house mouse

characteristic: description

text: adult

text: normal

text: LDLr-/-: Rag2-/-

characteristic: genotype

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spleen

text: Mus musculus

text: female

text: Sample 3

characteristic: title


E-MTAB-11677:Sample 4

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Bioinformatician

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11677:Sample 4

text: ArrayExpress

characteristic: broker name

text: marginal zone B cell

text: house mouse

characteristic: common name

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: LDLr-/-: Rag2-/-

text: not applicable

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: female

text: Sample 4


E-MTAB-11677:Sample 5

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Bioinformatician

text: public

characteristic: INSDC status

text: E-MTAB-11677:Sample 5

text: ArrayExpress

text: marginal zone B cell

text: house mouse

text: adult

text: normal

text: LDLr-/-: Rag2-/-

characteristic: growth condition

characteristic: individual

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: spleen

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

characteristic: strain

text: Sample 5


E-MTAB-11677:Sample 6

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Bioinformatician

text: public

characteristic: SRA accession

text: E-MTAB-11677:Sample 6

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: marginal zone B cell

text: house mouse

characteristic: description

text: adult

text: normal

text: LDLr-/-: Rag2-/-

characteristic: genotype

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spleen

text: Mus musculus

text: female

text: Sample 6

characteristic: title


E-MTAB-11677:Sample 7

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Bioinformatician

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11677:Sample 7

text: ArrayExpress

characteristic: broker name

text: marginal zone B cell

text: house mouse

characteristic: common name

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: not applicable

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: male

text: Sample 7


E-MTAB-11677:Sample 8

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Bioinformatician

text: public

characteristic: INSDC status

text: E-MTAB-11677:Sample 8

text: ArrayExpress

text: marginal zone B cell

text: house mouse

text: adult

text: normal

text: 82% CD4Cre/+; Bcl6flox/flox + 20% CD4+/+; Bcl6flox/flox (WT)

characteristic: growth condition

characteristic: individual

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: spleen

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex

characteristic: strain

text: Sample 8


E-MTAB-11677:Sample 9

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Bioinformatician

text: public

characteristic: SRA accession

text: E-MTAB-11677:Sample 9

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: marginal zone B cell

text: house mouse

characteristic: description

text: adult

text: normal

text: 84% CD4Cre/+; Bcl6flox/flox + 20% CD4+/+; Bcl6flox/flox (WT)

characteristic: genotype

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spleen

text: Mus musculus

text: female

text: Sample 9

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Rausing Laboratory Lund University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

tag: Namespace:Rausing Laboratory Lund University

text: ArrayExpress

characteristic: broker name

text: Norway rat

characteristic: common name

text: Protocols: Rats are 10 weeks old, female Fisher 344 rats at the initiation of the experiments. Tumor cell line: NS1 cells -  can be syngeneically inoculated into Fischer 344 rats where it generates infiltrative tumors, described by Ahlstedt, J., et al., Growth pattern of experimental glioblastoma. Histol Histopathol, 2020. 35(8): p. 871-886.Intracranial tumor: Each rat received 5000 NS1 glioblastoma cells, suspended in 5 µl of serum free nutrient solution. A stereotactic frame and a 10 µl Hamilton syringe. Isofluran anaesthesia was applied. The cells were injected on the right side of the cranium at a depth of 5 mm, 2 mm laterally from the sagittal suture, and 1 mm anterior to the coronal suture. The cranial burr hole was sealed with bone wax, and the incision was closed with absorbable suture.Subcutaneous tumor: Rats were inoculated with 50 000 NS1 cells subcutaneously on their hindleg and tumor site was carefully marked. Upon tumor development, a part of the tumor was dissected and frozen in liquid nitrogen when the. RNA extraction was performed using RNeasy kit (Qiagen®) according to the manufacturer’s instructions. All experiments have been approved by the local ethics board and have been conducted in accordance with the regulations. Tumors were collected from rats with intracranial or subcutaneous glioma. RNA was extracted using RNeasy kit (Qiagen®) according to manufacturer's instructions. a. Protocol: Qubit Flex Fluorometer Quick Reference No. MAN0018187 Rev. A.0 b. Reagent/kit: QuantIT RNA HS Assay Kit (Q33140, Life Technologies) c. Equipment: Qubit Flex (Q33327, Invitrogen Thermo Fisher Scientific) III. Library preparation: a. Protocol: TruSeq® Stranded mRNA Sample Preparation Guide (Part # 15031047 Rev. E) Modification of “Enrich DNA Fragment” step: from 15 to 13 PCR cycles b. Reagent/kit: TruSeq® Stranded mRNA Library Prep (20020594, Illumina) c. Index: IDT for Illumina TruSeq RNA UD Indexes (96 indexes, 20022371, Illumina) d. Equipment: Automatisation of Clean up steps: King Fisher FLEX (18-5400620, Thermo Scientific), incubations and PCR: Eppendorf Mastercycler X50s (6311000010, Eppendorf) IV. Library concentration check a. Protocol: Qubit Flex Fluorometer Quick Reference No. MAN0018187 Rev. A.0 b. Reagent/kit: QuantIT 1X dsDNA HS Assay Kit (Q33232) c. Equipment: Qubit Flex (Q33327 Invitrogen Thermo Fisher Scientific) V. Library size and quality check a. Protocol: Agilent D5000 ScreenTape Quick Guide for TapeStation, Part Number: G2991-90150 Edition 08/2015 b. Reagent/kit: D5000 Screen Tape (5067-5588, Agilent) c. Equipment: Agilent 2200 TapeStation System (G2964AA, Agilent)

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

text: in vivo transfection of NS1 cells intracranially until neoplasm

text: not applicable

text: Rattus norvegicus

text: Rattus norvegicus

text: brain

text: neoplasm

characteristic: sampling site

text: Rattus norvegicus

text: female

text: Fischer 344


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Rausing Laboratory Lund University

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: Norway rat

text: adult

text: normal

text: wild type genotype

text: in vivo transfection of NS1 cells subcutaneously until neoplasm

characteristic: growth condition

text: not applicable

characteristic: isolate

text: Rattus norvegicus

text: Rattus norvegicus

text: skin of hindleg

characteristic: organism part

text: neoplasm

text: Rattus norvegicus

text: female

characteristic: sex

text: Fischer 344

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: FISABIO

characteristic: INSDC center name

text: public

characteristic: Submitter Id

text: human gut metagenome

text: DNA metagenome from subject 000004-01

characteristic: description

text: terrestrial biome

characteristic: environment (biome)

text: human-associated habitat

characteristic: environment (feature)

text: human-associated habitat

characteristic: environment (material)

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

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