characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

text: CH-03-FO-SP1 bulk soil

text: Switzerland

text: Bagnes, Valais

text: uncultured eukaryote

characteristic: pH method

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: soil sieving

characteristic: sample material processing

characteristic: sample weight for DNA extraction

text: uncultured eukaryote

text: MiSeq

characteristic: sequencing method

characteristic: slope aspect

characteristic: slope gradient

text: in LifeGuard® (Qiagen) at -20degC

characteristic: storage conditions (fresh/frozen/other)

characteristic: target gene

characteristic: target subfragment

characteristic: total organic C method

characteristic: total organic carbon

characteristic: water content

text: heating at 105degC for 24 hours

characteristic: water content method


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:University of Neuchatel

characteristic: Submitter Id

unit: m

characteristic: altitude

text: temperate mixed forest biome [ENVO:01000212]

characteristic: broad-scale environmental context

characteristic: collection date

text: pool of ten subsample of 100 g and sieved at 2 mm mesh size

characteristic: composite design/sieving (if any)

characteristic: depth

text: CH-03-FO-SP2 bulk soil

characteristic: description

characteristic: elevation

text: soil [ENVO:00001998]|plant litter [ENVO:01000628]

characteristic: environmental medium

text: Switzerland

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Bagnes, Valais

characteristic: geographic location (region and locality)

text: soil surface layer [ENVO:04000010]|litter layer [ENVO:01000338]

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: organism

characteristic: pH

text: five grams of dry soil mixed in 12.5 ml of distilled water

text: initial denaturation=95degC_5min; 35 cycles:(denaturation=94degC_30sec; annealing=47degC_45sec; elongation=72degC_1min); final elongation=72degC_10min

text: TAReuk454FWD1:CCAGCASCYGCGGTAATTCC; TAReukREV3:ACTTTCGTTCTTGATYRA

text: Soil protists spatio-temporal turnover in mountains

text: soil sieving

unit: g

text: uncultured eukaryote

text: MiSeq

text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: Thermo ScientificTM FLASH 2000 CHNS elemental analyzer

unit: g/kg

unit: g/g

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

text: CH-03-FO-SP3 bulk soil

text: Switzerland

text: Bagnes, Valais

text: uncultured eukaryote

characteristic: pH method

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: soil sieving

characteristic: sample material processing

characteristic: sample weight for DNA extraction

text: uncultured eukaryote

text: MiSeq

characteristic: sequencing method

characteristic: slope aspect

characteristic: slope gradient

text: in LifeGuard® (Qiagen) at -20degC

characteristic: storage conditions (fresh/frozen/other)

characteristic: target gene

characteristic: target subfragment

characteristic: total organic C method

characteristic: total organic carbon

characteristic: water content

text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: CH-03-FO-SP4 bulk soil

characteristic: description

characteristic: elevation

characteristic: environmental medium

text: Switzerland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Bagnes, Valais

characteristic: geographic location (region and locality)

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: organism

characteristic: pH

text: soil sieving

text: uncultured eukaryote

text: MiSeq

text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

text: CH-03-FO-SP5 bulk soil

text: Switzerland

text: Bagnes, Valais

text: uncultured eukaryote

characteristic: pH method

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: soil sieving

characteristic: sample material processing

characteristic: sample weight for DNA extraction

text: uncultured eukaryote

text: MiSeq

characteristic: sequencing method

characteristic: slope aspect

characteristic: slope gradient

text: in LifeGuard® (Qiagen) at -20degC

characteristic: storage conditions (fresh/frozen/other)

characteristic: target gene

characteristic: target subfragment

characteristic: total organic C method

characteristic: total organic carbon

characteristic: water content

text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: CH-03-FO-SU1 bulk soil

characteristic: description

characteristic: elevation

characteristic: environmental medium

text: Switzerland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Bagnes, Valais

characteristic: geographic location (region and locality)

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: organism

characteristic: pH

text: soil sieving

text: uncultured eukaryote

text: MiSeq

text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

text: CH-03-FO-SU2 bulk soil

text: Switzerland

text: Bagnes, Valais

text: uncultured eukaryote

characteristic: pH method

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: soil sieving

characteristic: sample material processing

characteristic: sample weight for DNA extraction

text: uncultured eukaryote

text: MiSeq

characteristic: sequencing method

characteristic: slope aspect

characteristic: slope gradient

text: in LifeGuard® (Qiagen) at -20degC

characteristic: storage conditions (fresh/frozen/other)

characteristic: target gene

characteristic: target subfragment

characteristic: total organic C method

characteristic: total organic carbon

characteristic: water content

text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: CH-03-FO-SU3 bulk soil

characteristic: description

characteristic: elevation

characteristic: environmental medium

text: Switzerland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Bagnes, Valais

characteristic: geographic location (region and locality)

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: organism

characteristic: pH

text: soil sieving

text: uncultured eukaryote

text: MiSeq

text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

text: CH-03-FO-SU5 bulk soil

text: Switzerland

text: Bagnes, Valais

text: uncultured eukaryote

characteristic: pH method

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: soil sieving

characteristic: sample material processing

characteristic: sample weight for DNA extraction

text: uncultured eukaryote

text: MiSeq

characteristic: sequencing method

characteristic: slope aspect

characteristic: slope gradient

text: in LifeGuard® (Qiagen) at -20degC

characteristic: storage conditions (fresh/frozen/other)

characteristic: target gene

characteristic: target subfragment

characteristic: total organic C method

characteristic: total organic carbon

characteristic: water content

text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

text: temperate grassland biome [ENVO:01000193]

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: CH-03-OP-SP1 bulk soil

characteristic: description

characteristic: elevation

text: soil [ENVO:00001998]

characteristic: environmental medium

text: Switzerland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Orsières, Valais

characteristic: geographic location (region and locality)

text: soil surface layer [ENVO:04000010]

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: organism

characteristic: pH

text: soil sieving

text: uncultured eukaryote

text: MiSeq

text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

text: CH-03-OP-SP2 bulk soil

text: soil [ENVO:00001998]

text: Switzerland

text: Liddes, Valais

text: soil surface layer [ENVO:04000010]

text: uncultured eukaryote

characteristic: pH method

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: soil sieving

characteristic: sample material processing

characteristic: sample weight for DNA extraction

text: uncultured eukaryote

text: MiSeq

characteristic: sequencing method

characteristic: slope aspect

characteristic: slope gradient

text: in LifeGuard® (Qiagen) at -20degC

characteristic: storage conditions (fresh/frozen/other)

characteristic: target gene

characteristic: target subfragment

characteristic: total organic C method

characteristic: total organic carbon

characteristic: water content

text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: CH-03-OP-SP3 bulk soil

characteristic: description

characteristic: elevation

text: soil [ENVO:00001998]

characteristic: environmental medium

text: Switzerland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Bagnes, Valais

characteristic: geographic location (region and locality)

text: soil surface layer [ENVO:04000010]

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: organism

characteristic: pH

text: soil sieving

text: uncultured eukaryote

text: MiSeq

text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

text: CH-03-OP-SP4 bulk soil

text: soil [ENVO:00001998]

text: Switzerland

text: Orsières, Valais

text: soil surface layer [ENVO:04000010]

text: uncultured eukaryote

characteristic: pH method

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: soil sieving

characteristic: sample material processing

characteristic: sample weight for DNA extraction

text: uncultured eukaryote

text: MiSeq

characteristic: sequencing method

characteristic: slope aspect

characteristic: slope gradient

text: in LifeGuard® (Qiagen) at -20degC

characteristic: storage conditions (fresh/frozen/other)

characteristic: target gene

characteristic: target subfragment

characteristic: total organic C method

characteristic: total organic carbon

characteristic: water content

text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: CH-03-OP-SP5 bulk soil

characteristic: description

characteristic: elevation

text: soil [ENVO:00001998]

characteristic: environmental medium

text: Switzerland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Orsières, Valais

characteristic: geographic location (region and locality)

text: soil surface layer [ENVO:04000010]

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: organism

characteristic: pH

text: soil sieving

text: uncultured eukaryote

text: MiSeq

text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

text: CH-03-OP-SU1 bulk soil

text: soil [ENVO:00001998]

text: Switzerland

text: Orsières, Valais

text: soil surface layer [ENVO:04000010]

text: uncultured eukaryote

characteristic: pH method

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: soil sieving

characteristic: sample material processing

characteristic: sample weight for DNA extraction

text: uncultured eukaryote

text: MiSeq

characteristic: sequencing method

characteristic: slope aspect

characteristic: slope gradient

text: in LifeGuard® (Qiagen) at -20degC

characteristic: storage conditions (fresh/frozen/other)

characteristic: target gene

characteristic: target subfragment

characteristic: total organic C method

characteristic: total organic carbon

characteristic: water content

text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: CH-03-OP-SU2 bulk soil

characteristic: description

characteristic: elevation

text: soil [ENVO:00001998]

characteristic: environmental medium

text: Switzerland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Liddes, Valais

characteristic: geographic location (region and locality)

text: soil surface layer [ENVO:04000010]

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: organism

characteristic: pH

text: soil sieving

text: uncultured eukaryote

text: MiSeq

text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

text: CH-03-OP-SU3 bulk soil

text: soil [ENVO:00001998]

text: Switzerland

text: Bagnes, Valais

text: soil surface layer [ENVO:04000010]

text: uncultured eukaryote

characteristic: pH method

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: soil sieving

characteristic: sample material processing

characteristic: sample weight for DNA extraction

text: uncultured eukaryote

text: MiSeq

characteristic: sequencing method

characteristic: slope aspect

characteristic: slope gradient

text: in LifeGuard® (Qiagen) at -20degC

characteristic: storage conditions (fresh/frozen/other)

characteristic: target gene

characteristic: target subfragment

characteristic: total organic C method

characteristic: total organic carbon

characteristic: water content

text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: CH-03-OP-SU4 bulk soil

characteristic: description

characteristic: elevation

text: soil [ENVO:00001998]

characteristic: environmental medium

text: Switzerland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Orsières, Valais

characteristic: geographic location (region and locality)

text: soil surface layer [ENVO:04000010]

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: organism

characteristic: pH

text: soil sieving

text: uncultured eukaryote

text: MiSeq

text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

text: CH-03-OP-SU5 bulk soil

text: soil [ENVO:00001998]

text: Switzerland

text: Orsières, Valais

text: soil surface layer [ENVO:04000010]

text: uncultured eukaryote

characteristic: pH method

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: soil sieving

characteristic: sample material processing

characteristic: sample weight for DNA extraction

text: uncultured eukaryote

text: MiSeq

characteristic: sequencing method

characteristic: slope aspect

characteristic: slope gradient

text: in LifeGuard® (Qiagen) at -20degC

characteristic: storage conditions (fresh/frozen/other)

characteristic: target gene

characteristic: target subfragment

characteristic: total organic C method

characteristic: total organic carbon

characteristic: water content

text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

text: mediterranean forest biome [ENVO:01000199]

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: SN-05-FO-SP1 bulk soil

characteristic: description

characteristic: elevation

characteristic: environmental medium

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Cenes de la Vega, Andalucia

characteristic: geographic location (region and locality)

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: organism

characteristic: pH

text: soil sieving

text: uncultured eukaryote

text: MiSeq

text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

text: SN-05-FO-SP2 bulk soil

text: Spain

text: Pinos Genil, Andalucia

text: uncultured eukaryote

characteristic: pH method

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: soil sieving

characteristic: sample material processing

characteristic: sample weight for DNA extraction

text: uncultured eukaryote

text: MiSeq

characteristic: sequencing method

characteristic: slope aspect

characteristic: slope gradient

text: in LifeGuard® (Qiagen) at -20degC

characteristic: storage conditions (fresh/frozen/other)

characteristic: target gene

characteristic: target subfragment

characteristic: total organic C method

characteristic: total organic carbon

characteristic: water content

text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: SN-05-FO-SP3 bulk soil

characteristic: description

characteristic: elevation

characteristic: environmental medium

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Güéjar-Sierra, Andalucia

characteristic: geographic location (region and locality)

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: organism

characteristic: pH

text: soil sieving

text: uncultured eukaryote

text: MiSeq

text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

text: SN-05-FO-SP4 bulk soil

text: Spain

text: Pradollano, Andalucia

text: uncultured eukaryote

characteristic: pH method

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: soil sieving

characteristic: sample material processing

characteristic: sample weight for DNA extraction

text: uncultured eukaryote

text: MiSeq

characteristic: sequencing method

characteristic: slope aspect

characteristic: slope gradient

text: in LifeGuard® (Qiagen) at -20degC

characteristic: storage conditions (fresh/frozen/other)

characteristic: target gene

characteristic: target subfragment

characteristic: total organic C method

characteristic: total organic carbon

characteristic: water content

text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: SN-05-FO-SP5 bulk soil

characteristic: description

characteristic: elevation

characteristic: environmental medium

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Pradollano, Andalucia

characteristic: geographic location (region and locality)

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: organism

characteristic: pH

text: soil sieving

text: uncultured eukaryote

text: MiSeq

text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

text: SN-05-FO-SU1 bulk soil

text: Spain

text: Cenes de la Vega, Andalucia

text: uncultured eukaryote

characteristic: pH method

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: soil sieving

characteristic: sample material processing

characteristic: sample weight for DNA extraction

text: uncultured eukaryote

text: MiSeq

characteristic: sequencing method

characteristic: slope aspect

characteristic: slope gradient

text: in LifeGuard® (Qiagen) at -20degC

characteristic: storage conditions (fresh/frozen/other)

characteristic: target gene

characteristic: target subfragment

characteristic: total organic C method

characteristic: total organic carbon

characteristic: water content

text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: SN-05-FO-SU2 bulk soil

characteristic: description

characteristic: elevation

characteristic: environmental medium

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Pinos Genil, Andalucia

characteristic: geographic location (region and locality)

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: organism

characteristic: pH

text: soil sieving

text: uncultured eukaryote

text: MiSeq

text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

text: SN-05-FO-SU3 bulk soil

text: Spain

text: Güéjar-Sierra, Andalucia

text: uncultured eukaryote

characteristic: pH method

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: soil sieving

characteristic: sample material processing

characteristic: sample weight for DNA extraction

text: uncultured eukaryote

text: MiSeq

characteristic: sequencing method

characteristic: slope aspect

characteristic: slope gradient

text: in LifeGuard® (Qiagen) at -20degC

characteristic: storage conditions (fresh/frozen/other)

characteristic: target gene

characteristic: target subfragment

characteristic: total organic C method

characteristic: total organic carbon

characteristic: water content

text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: SN-05-FO-SU5 bulk soil

characteristic: description

characteristic: elevation

characteristic: environmental medium

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Pradollano, Andalucia

characteristic: geographic location (region and locality)

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: organism

characteristic: pH

text: soil sieving

text: uncultured eukaryote

text: MiSeq

text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

text: mediterranean grassland biome [ENVO:01000224]

text: SN-05-OP-SP1 bulk soil

text: soil [ENVO:00001998]

text: Spain

text: Monachil, Andalucia

text: soil surface layer [ENVO:04000010]

text: uncultured eukaryote

characteristic: pH method

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: soil sieving

characteristic: sample material processing

characteristic: sample weight for DNA extraction

text: uncultured eukaryote

text: MiSeq

characteristic: sequencing method

characteristic: slope aspect

characteristic: slope gradient

text: in LifeGuard® (Qiagen) at -20degC

characteristic: storage conditions (fresh/frozen/other)

characteristic: target gene

characteristic: target subfragment

characteristic: total organic C method

characteristic: total organic carbon

characteristic: water content

text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: SN-05-OP-SP2 bulk soil

characteristic: description

characteristic: elevation

text: soil [ENVO:00001998]

characteristic: environmental medium

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: El Purche, Andalucia

characteristic: geographic location (region and locality)

text: soil surface layer [ENVO:04000010]

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: organism

characteristic: pH

text: soil sieving

text: uncultured eukaryote

text: MiSeq

text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

text: SN-05-OP-SP3 bulk soil

text: soil [ENVO:00001998]

text: Spain

text: El Purche, Andalucia

text: soil surface layer [ENVO:04000010]

text: uncultured eukaryote

characteristic: pH method

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: soil sieving

characteristic: sample material processing

characteristic: sample weight for DNA extraction

text: uncultured eukaryote

text: MiSeq

characteristic: sequencing method

characteristic: slope aspect

characteristic: slope gradient

text: in LifeGuard® (Qiagen) at -20degC

characteristic: storage conditions (fresh/frozen/other)

characteristic: target gene

characteristic: target subfragment

characteristic: total organic C method

characteristic: total organic carbon

characteristic: water content

text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: SN-05-OP-SP4 bulk soil

characteristic: description

characteristic: elevation

text: soil [ENVO:00001998]

characteristic: environmental medium

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Güéjar-Sierra, Andalucia

characteristic: geographic location (region and locality)

text: soil surface layer [ENVO:04000010]

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: organism

characteristic: pH

text: soil sieving

text: uncultured eukaryote

text: MiSeq

text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

text: SN-05-OP-SP5 bulk soil

text: soil [ENVO:00001998]

text: Spain

text: Pradollano, Andalucia

text: soil surface layer [ENVO:04000010]

text: uncultured eukaryote

characteristic: pH method

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: soil sieving

characteristic: sample material processing

characteristic: sample weight for DNA extraction

text: uncultured eukaryote

text: MiSeq

characteristic: sequencing method

characteristic: slope aspect

characteristic: slope gradient

text: in LifeGuard® (Qiagen) at -20degC

characteristic: storage conditions (fresh/frozen/other)

characteristic: target gene

characteristic: target subfragment

characteristic: total organic C method

characteristic: total organic carbon

characteristic: water content

text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: SN-05-OP-SP6 bulk soil

characteristic: description

characteristic: elevation

text: soil [ENVO:00001998]

characteristic: environmental medium

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Sierra Nevada, Andalucia

characteristic: geographic location (region and locality)

text: soil surface layer [ENVO:04000010]

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: organism

characteristic: pH

text: soil sieving

text: uncultured eukaryote

text: MiSeq

text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

text: SN-05-OP-SU1 bulk soil

text: soil [ENVO:00001998]

text: Spain

text: Cenes de la Vega, Andalucia

text: soil surface layer [ENVO:04000010]

text: uncultured eukaryote

characteristic: pH method

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: soil sieving

characteristic: sample material processing

characteristic: sample weight for DNA extraction

text: uncultured eukaryote

text: MiSeq

characteristic: sequencing method

characteristic: slope aspect

characteristic: slope gradient

text: in LifeGuard® (Qiagen) at -20degC

characteristic: storage conditions (fresh/frozen/other)

characteristic: target gene

characteristic: target subfragment

characteristic: total organic C method

characteristic: total organic carbon

characteristic: water content

text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: SN-05-OP-SU2 bulk soil

characteristic: description

characteristic: elevation

text: soil [ENVO:00001998]

characteristic: environmental medium

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: El Purche, Andalucia

characteristic: geographic location (region and locality)

text: soil surface layer [ENVO:04000010]

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: organism

characteristic: pH

text: soil sieving

text: uncultured eukaryote

text: MiSeq

text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

text: SN-05-OP-SU3 bulk soil

text: soil [ENVO:00001998]

text: Spain

text: El Purche, Andalucia

text: soil surface layer [ENVO:04000010]

text: uncultured eukaryote

characteristic: pH method

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: soil sieving

characteristic: sample material processing

characteristic: sample weight for DNA extraction

text: uncultured eukaryote

text: MiSeq

characteristic: sequencing method

characteristic: slope aspect

characteristic: slope gradient

text: in LifeGuard® (Qiagen) at -20degC

characteristic: storage conditions (fresh/frozen/other)

characteristic: target gene

characteristic: target subfragment

characteristic: total organic C method

characteristic: total organic carbon

characteristic: water content

text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: SN-05-OP-SU4 bulk soil

characteristic: description

characteristic: elevation

text: soil [ENVO:00001998]

characteristic: environmental medium

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Güéjar-Sierra, Andalucia

characteristic: geographic location (region and locality)

text: soil surface layer [ENVO:04000010]

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: organism

characteristic: pH

text: soil sieving

text: uncultured eukaryote

text: MiSeq

text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

text: SN-05-OP-SU5 bulk soil

text: soil [ENVO:00001998]

text: Spain

text: Pradollano, Andalucia

text: soil surface layer [ENVO:04000010]

text: uncultured eukaryote

characteristic: pH method

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: soil sieving

characteristic: sample material processing

characteristic: sample weight for DNA extraction

text: uncultured eukaryote

text: MiSeq

characteristic: sequencing method

characteristic: slope aspect

characteristic: slope gradient

text: in LifeGuard® (Qiagen) at -20degC

characteristic: storage conditions (fresh/frozen/other)

characteristic: target gene

characteristic: target subfragment

characteristic: total organic C method

characteristic: total organic carbon

characteristic: water content

text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: SN-05-OP-SU6 bulk soil

characteristic: description

characteristic: elevation

text: soil [ENVO:00001998]

characteristic: environmental medium

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Sierra Nevada, Andalucia

characteristic: geographic location (region and locality)

text: soil surface layer [ENVO:04000010]

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: organism

characteristic: pH

text: soil sieving

text: uncultured eukaryote

text: MiSeq

text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

text: SN-06-FO-SP1 bulk soil

text: Spain

text: Carataunas, Andalucia

text: uncultured eukaryote

characteristic: pH method

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: soil sieving

characteristic: sample material processing

characteristic: sample weight for DNA extraction

text: uncultured eukaryote

text: MiSeq

characteristic: sequencing method

characteristic: slope aspect

characteristic: slope gradient

text: in LifeGuard® (Qiagen) at -20degC

characteristic: storage conditions (fresh/frozen/other)

characteristic: target gene

characteristic: target subfragment

characteristic: total organic C method

characteristic: total organic carbon

characteristic: water content

text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: SN-06-FO-SP2 bulk soil

characteristic: description

characteristic: elevation

characteristic: environmental medium

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Pampaneira, Andalucia

characteristic: geographic location (region and locality)

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: organism

characteristic: pH

text: soil sieving

text: uncultured eukaryote

text: MiSeq

text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

text: SN-06-FO-SP3 bulk soil

text: Spain

text: Capileira, Andalucia

text: uncultured eukaryote

characteristic: pH method

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: soil sieving

characteristic: sample material processing

characteristic: sample weight for DNA extraction

text: uncultured eukaryote

text: MiSeq

characteristic: sequencing method

characteristic: slope aspect

characteristic: slope gradient

text: in LifeGuard® (Qiagen) at -20degC

characteristic: storage conditions (fresh/frozen/other)

characteristic: target gene

characteristic: target subfragment

characteristic: total organic C method

characteristic: total organic carbon

characteristic: water content

text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: SN-06-FO-SP4 bulk soil

characteristic: description

characteristic: elevation

characteristic: environmental medium

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Capileira, Andalucia

characteristic: geographic location (region and locality)

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: organism

characteristic: pH

text: soil sieving

text: uncultured eukaryote

text: MiSeq

text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

text: SN-06-FO-SU1 bulk soil

text: Spain

text: Carataunas, Andalucia

text: uncultured eukaryote

characteristic: pH method

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: soil sieving

characteristic: sample material processing

characteristic: sample weight for DNA extraction

text: uncultured eukaryote

text: MiSeq

characteristic: sequencing method

characteristic: slope aspect

characteristic: slope gradient

text: in LifeGuard® (Qiagen) at -20degC

characteristic: storage conditions (fresh/frozen/other)

characteristic: target gene

characteristic: target subfragment

characteristic: total organic C method

characteristic: total organic carbon

characteristic: water content

text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: SN-06-FO-SU2 bulk soil

characteristic: description

characteristic: elevation

characteristic: environmental medium

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Pampaneira, Andalucia

characteristic: geographic location (region and locality)

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: organism

characteristic: pH

text: soil sieving

text: uncultured eukaryote

text: MiSeq

text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

text: SN-06-OP-SP1 bulk soil

text: soil [ENVO:00001998]

text: Spain

text: Carataunas, Andalucia

text: soil surface layer [ENVO:04000010]

text: uncultured eukaryote

characteristic: pH method

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: soil sieving

characteristic: sample material processing

characteristic: sample weight for DNA extraction

text: uncultured eukaryote

text: MiSeq

characteristic: sequencing method

characteristic: slope aspect

characteristic: slope gradient

text: in LifeGuard® (Qiagen) at -20degC

characteristic: storage conditions (fresh/frozen/other)

characteristic: target gene

characteristic: target subfragment

characteristic: total organic C method

characteristic: total organic carbon

characteristic: water content

text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: SN-06-OP-SP2 bulk soil

characteristic: description

characteristic: elevation

text: soil [ENVO:00001998]

characteristic: environmental medium

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Pampaneira, Andalucia

characteristic: geographic location (region and locality)

text: soil surface layer [ENVO:04000010]

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: organism

characteristic: pH

text: soil sieving

text: uncultured eukaryote

text: MiSeq

text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

text: SN-06-OP-SP3 bulk soil

text: soil [ENVO:00001998]

text: Spain

text: Capileira, Andalucia

text: soil surface layer [ENVO:04000010]

text: uncultured eukaryote

characteristic: pH method

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: soil sieving

characteristic: sample material processing

characteristic: sample weight for DNA extraction

text: uncultured eukaryote

text: MiSeq

characteristic: sequencing method

characteristic: slope aspect

characteristic: slope gradient

text: in LifeGuard® (Qiagen) at -20degC

characteristic: storage conditions (fresh/frozen/other)

characteristic: target gene

characteristic: target subfragment

characteristic: total organic C method

characteristic: total organic carbon

characteristic: water content

text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: SN-06-OP-SP4 bulk soil

characteristic: description

characteristic: elevation

text: soil [ENVO:00001998]

characteristic: environmental medium

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Capileira, Andalucia

characteristic: geographic location (region and locality)

text: soil surface layer [ENVO:04000010]

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: organism

characteristic: pH

text: soil sieving

text: uncultured eukaryote

text: MiSeq

text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

text: SN-06-OP-SP5 bulk soil

text: soil [ENVO:00001998]

text: Spain

text: Pórtugos, Andalucia

text: soil surface layer [ENVO:04000010]

text: uncultured eukaryote

characteristic: pH method

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: soil sieving

characteristic: sample material processing

characteristic: sample weight for DNA extraction

text: uncultured eukaryote

text: MiSeq

characteristic: sequencing method

characteristic: slope aspect

characteristic: slope gradient

text: in LifeGuard® (Qiagen) at -20degC

characteristic: storage conditions (fresh/frozen/other)

characteristic: target gene

characteristic: target subfragment

characteristic: total organic C method

characteristic: total organic carbon

characteristic: water content

text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: SN-06-OP-SU1 bulk soil

characteristic: description

characteristic: elevation

text: soil [ENVO:00001998]

characteristic: environmental medium

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Carataunas, Andalucia

characteristic: geographic location (region and locality)

text: soil surface layer [ENVO:04000010]

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: organism

characteristic: pH

text: soil sieving

text: uncultured eukaryote

text: MiSeq

text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

text: SN-06-OP-SU2 bulk soil

text: soil [ENVO:00001998]

text: Spain

text: Pampaneira, Andalucia

text: soil surface layer [ENVO:04000010]

text: uncultured eukaryote

characteristic: pH method

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: soil sieving

characteristic: sample material processing

characteristic: sample weight for DNA extraction

text: uncultured eukaryote

text: MiSeq

characteristic: sequencing method

characteristic: slope aspect

characteristic: slope gradient

text: in LifeGuard® (Qiagen) at -20degC

characteristic: storage conditions (fresh/frozen/other)

characteristic: target gene

characteristic: target subfragment

characteristic: total organic C method

characteristic: total organic carbon

characteristic: water content

text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: SN-06-OP-SU3 bulk soil

characteristic: description

characteristic: elevation

text: soil [ENVO:00001998]

characteristic: environmental medium

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Capileira, Andalucia

characteristic: geographic location (region and locality)

text: soil surface layer [ENVO:04000010]

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: organism

characteristic: pH

text: soil sieving

text: uncultured eukaryote

text: MiSeq

text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

text: SN-06-OP-SU4 bulk soil

text: soil [ENVO:00001998]

text: Spain

text: Capileira, Andalucia

text: soil surface layer [ENVO:04000010]

text: uncultured eukaryote

characteristic: pH method

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: soil sieving

characteristic: sample material processing

characteristic: sample weight for DNA extraction

text: uncultured eukaryote

text: MiSeq

characteristic: sequencing method

characteristic: slope aspect

characteristic: slope gradient

text: in LifeGuard® (Qiagen) at -20degC

characteristic: storage conditions (fresh/frozen/other)

characteristic: target gene

characteristic: target subfragment

characteristic: total organic C method

characteristic: total organic carbon

characteristic: water content

text: heating at 105degC for 24 hours

characteristic: water content method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Neuchatel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: composite design/sieving (if any)

characteristic: depth

text: SN-06-OP-SU5 bulk soil

characteristic: description

characteristic: elevation

text: soil [ENVO:00001998]

characteristic: environmental medium

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Pórtugos, Andalucia

characteristic: geographic location (region and locality)

text: soil surface layer [ENVO:04000010]

characteristic: local environmental context

characteristic: negative control type

text: uncultured eukaryote

characteristic: organism

characteristic: pH

text: soil sieving

text: uncultured eukaryote

text: MiSeq

text: in LifeGuard® (Qiagen) at -20degC

characteristic: title

text: heating at 105degC for 24 hours


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:The Department of Earth and Environmental Sciences at BGU

characteristic: Submitter Id

text: lake [ENVO_00000020]

text: Microbial methanoges-methanotrophs and iron reducers in incubation experiments from lake sediment

text: Israel

text: North Israel The sea of galilee

text: lake sediment (ENVO_00000546)

text: uncultured prokaryote

text: Microbial methanoges-methanotrophs and iron reducers in incubation experiments of sediments from the sea of Galilee

characteristic: project name

text: uncultured prokaryote

text: Incubation of lake sediment control


ExpD-methane

text: The Department of Earth and Environmental Sciences at BGU

text: public

text: ExpD-methane

text: lake [ENVO_00000020]

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: elevation

characteristic: environmental medium

text: Israel

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: North Israel The sea of galilee

text: lake sediment (ENVO_00000546)

text: uncultured prokaryote

text: uncultured prokaryote

text: Incubation of lake sediment methane addition


expD-methanol

characteristic: ENA first public

text: public

text: expD-methanol

text: lake [ENVO_00000020]

text: Israel

characteristic: geographic location (longitude)

text: North Israel The sea of galilee

characteristic: geographic location (region and locality)

text: lake sediment (ENVO_00000546)

characteristic: local environmental context

text: uncultured prokaryote

characteristic: organism

text: uncultured prokaryote

text: Incubation of lake sediment methanol addition

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: lake [ENVO_00000020]

text: Israel

text: North Israel The sea of galilee

text: lake sediment (ENVO_00000546)

text: uncultured prokaryote

text: uncultured prokaryote

text: Incubation of lake sediment control2


text: public

characteristic: Submitter Id

text: lake [ENVO_00000020]

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

characteristic: description

text: Israel

text: North Israel The sea of galilee

text: lake sediment (ENVO_00000546)

text: uncultured prokaryote

characteristic: project name

text: uncultured prokaryote

text: Incubation of lake sediment control3


ExpE-BES

characteristic: ENA first public

text: public

text: ExpE-BES

text: lake [ENVO_00000020]

characteristic: elevation

characteristic: environmental medium

text: Israel

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: North Israel The sea of galilee

text: lake sediment (ENVO_00000546)

text: uncultured prokaryote

text: uncultured prokaryote

text: Incubation of lake sediment BES addition


ExpE-hematite

characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

text: ExpE-hematite

text: lake [ENVO_00000020]

text: Israel

text: North Israel The sea of galilee

characteristic: geographic location (region and locality)

text: lake sediment (ENVO_00000546)

characteristic: local environmental context

text: uncultured prokaryote

characteristic: organism

text: uncultured prokaryote

text: Incubation of lake sediment hematite addition

characteristic: title


text: yes

characteristic: Comp

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: ETH Zurich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 1750 m

characteristic: Site

tag: Namespace:ETH Zurich

characteristic: Submitter Id

text: yes

characteristic: Transplant

text: Plantago alpina

text: Plantago alpina


text: yes

characteristic: ENA-CHECKLIST

text: ETH Zurich

text: public

text: 2200 m

text: yes

text: Plantago alpina

text: Plantago alpina


text: no

characteristic: External Id

text: ETH Zurich

text: public

text: 1950 m

text: yes

text: Plantago alpina

text: Plantago alpina


text: no

text: ETH Zurich

text: public

text: 1750 m

text: yes

text: Plantago alpina

text: Plantago alpina


text: yes

text: ETH Zurich

text: public

text: 1400 m

text: yes

text: Plantago alpina

text: Plantago alpina


text: no

text: ETH Zurich

text: public

text: 2200 m

text: yes

text: Plantago alpina

text: Plantago alpina


text: no

text: ETH Zurich

text: public

text: 1400 m

text: yes

text: Plantago alpina

text: Plantago alpina


text: yes

text: ETH Zurich

text: public

text: 1950 m

text: yes

text: Plantago alpina

text: Plantago alpina


text: yes

text: ETH Zurich

text: public

text: 2200 m

text: no

text: Plantago alpina

text: Plantago alpina


text: Shanghai Tenth People's Hospital

text: public

tag: Namespace:Shanghai Tenth People's Hospital

text: ArrayExpress

characteristic: broker name

text: Protocols: Cells were grown in YPD broth at 37˚C from OD600=0.01 till OD600=1.0. Cells were treated with 1 µg/ml or 8 µg/ml of tunicamycin, or DMSO (control) for 3h. Pellets of 5ml cell culture were obtained by centrifuging at maximum speed for 1min. the pellets were fast frozen in liquid nitrogen. RNA was extracted using the TRIzol method following the manufacturer's protocol. Cell pellets were transferred from liquid nitrogen into the 2ml tub containing 1.5ml Trizol reagent. The mix was shared for 3min, and kept for 5in at room temperature, then centrifuged at 10,000xg for 5min at 4˚C. The supernatant was added 200µl of chloroform/isoamyl alcohol (24:1) with 1ml lysis reagents. After centrifuged at 10,000g for 10min at 4˚C, the supernatant was transferred into another new tube with equal volume of isopropanol and put in the refrigerator at -20˚C for 1h. After centrifuged at 13600xg for 20min at 4˚C, the supernatant was precipitated by 1ml of 75% ethanol and let dry for 3-5min. The RNA pellet was dissolved with 30-100µl of DEPC water. The concentration of the extracted RNA was determined using a Nanodrop system (NanoDrop, Madison, USA), and the integrity of the RNA was examined by the RNA integrity number (RIN) using an Agilent 2100 bioanalyzer (Agilent, Santa Clara, USA) buffer at appropriate temperature. Then First-strand cDNA was generated using random hexamer-primed reverse transcription, followed by a second-strand cDNA synthesis. afterwards, A-Tailing Mix and RNA Index Adapters were added by incubating to end repair. The cDNA fragments obtained from previous step were amplified by PCR, and products were purified by Ampure XP Beads, then dissolved in EB solution. The product was validated on the Agilent Technologies 2100 bioanalyzer for quality control. The double stranded PCR products from previous step were heated denatured and circularized by the splint oligo sequence to get the final library. The single strand circle DNA (ssCir DNA) was formatted as the final library.

characteristic: description

text: wild type genotype

characteristic: genotype

text: grown to exponential phase

characteristic: growth condition

text: not applicable

characteristic: isolate

text: Candida auris

text: [Candida] auris

text: whole organism

characteristic: organism part

text: [Candida] auris

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF WARWICK

characteristic: INSDC center name

text: public

tag: Namespace:UNIVERSITY OF WARWICK

text: ArrayExpress

text: whole organism

text: Protocols: The Snapwell inserts containing the polarised cell layers were placed between the two half chambers of the VDC (Harvard Apparatus, Cambridge, UK) and sealed with the provided clamps. 3 mL DMEM-10 was added to both sides to fill the chamber. As described previously, (31) a single bacterial colony was inoculated in pre-reduced BHIS broth and incubated at 37°C for 16 h in anaerobic conditions. The culture was centrifuged at 5,000 rpm for 5 min (Eppendorf 5810R, Eppendorf, Germany) and bacterial pellet was resuspended in DMEM-10, before being diluted to an OD600 of 1.0 and incubated at 37°C in anaerobic conditions for 1 hour. Intestinal epithelial cells were infected with bacterial cultures at an MOI of 100:1 in the apical chamber. This chamber was supplied with anaerobic gas mixture (10% CO2, 10% H2, 80% N2, BOC, UK) and the basolateral compartment with 5% CO2 and 95% air (BOC, UK) at a rate of ~1 bubble every 5 seconds. At 3 h post infection, the apical media containing the C. difficile was removed, the chamber was washed once with PBS and 3 mL fresh pre-reduced DMEM-10 was added. Chambers were incubated for a further 3 – 45 h. The intestinal epithelial cell layers were washed thrice with pre-reduced PBS before being lysed with 1 mL sterile water. The number of cell-associated b and colony forming unit (CFU) counts from the intestinal epithelial cell lysate, prepared using serial dilutions plated on BHIS agar. Bacterial and human cells were treated with RNAprotect cell or bacterial reagent (Qiagen, Germany) and stored at -80°C for up to 1 week. To extract RNA, samples were thawed and centrifuged at 4°C (Sigma Zentrifugen 1-14K, Germany). Cell pellets were resuspended in 1 mL buffer RLT from the RNeasy mini kit (Qiagen, Germany) with a 1:200 dilution of β-mercaptoethanol (Sigma Aldrich, USA). Cells were homogenised using lysing matrix B tubes with 0.1 mm silica beads (MP Biomedicals, USA) in a FastPrep-24 5G (6.5 m/s for 20 seconds with 180 seconds rest on ice for 6 cycles) (MP Biomedicals, USA). RNA was extracted using the RNeasy RNA isolation kit, (Qiagen, Germany), according to the manufacturer’s protocol. A rigorous treatment with TURBO DNase (Thermo Fisher Scientific, USA) was used to remove genomic DNA contamination from RNA samples and clean up was performed using the RNeasy mini kit following the manufacturer’s protocol (Qiagen, Germany). RNA concentrations were quantified using the Qubit RNA BR assay kit (Thermo Fisher Scientific, USA). The RNA quality was examined using the Bioanalyzer RNA 6000 pico kit (Agilent, USA). An RNase H method for rRNA depletion was used as described by Adiconis et al. (2013). The human rRNA single-stranded DNA probes described in this thesis were purchased as oligonucleotides (Integrated DNA technologies, USA). C. difficile probes complementary to the 16S, 23S and 5S rRNA sequences were designed as ~50 bp non- overlapping primers (Integrated DNA Technologies, USA). RNase H rRNA depletion protocol was used as described by SciLifeLab (Sweden). Briefly, oligonucleotide probes were hybridised to RNA samples by incubation with hybridisation buffer (1 M NaCl, 0.5 M Tris-HCl pH 7.4) in a Bio-Rad T100™ thermocycler (95°C for 2 min, temperature decreased -0.1 C/s to 45°C and hold at 45°C). Thermostable RNase H (New England Biolabs, USA) was added, and the reaction was incubated at 45°C for 30 min. Agencourt® AMPure® XP magnetic beads were used to purify RNA. DNase I (New England Biolabs, USA) was used to remove leftover DNA oligonucleotides and Agencourt® AMPure® XP magnetic beads (Beckman Coulter Life Sciences, USA) were used to clean up rRNA depleted samples. Bioanalyzer RNA pico analysis was used to assess the RNA quality and effectiveness of the rRNA depletion (Agilent, USA). The NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® with the NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1) were used to generate DNA libraries for sequencing (New England Biolabs, USA) as per the manufacturers protocol. Agencourt® AMPure XP magnetic beads (Beckman Coulter Life Sciences, USA) were used for bead-based DNA clean up. Quality of DNA libraries were assessed using a Bioanalyzer High Sensitivity DNA kit (Agilent, USA).

text: normal

characteristic: disease

text: wild type genotype

text: monoculture

characteristic: infect

text: not applicable

text: Clostridioides difficile

characteristic: organism

text: Clostridioides difficile

text: whole organism

text: Clostridioides difficile

characteristic: title


text: UNIVERSITY OF WARWICK

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: intestinal epithelial cell

text: human

characteristic: common name

text: normal

text: wild type genotype

text: monoculture

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: intestinal epithelium

text: Homo sapiens


characteristic: ENA-CHECKLIST

characteristic: External Id

text: UNIVERSITY OF WARWICK

text: public

text: ArrayExpress

characteristic: broker name

text: intestinal epithelial cell

text: human

characteristic: description

text: normal

text: wild type genotype

characteristic: genotype

text: co-culture in snapwell with Clostridioides difficile

characteristic: growth condition

text: Clostridioides difficile

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: intestinal epithelium

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UMCG

characteristic: INSDC center name

text: public

text: Netherlands

characteristic: country

text: human

characteristic: host

text: Yes

text: throat swab

text: Staphylococcus aureus

text: Staphylococcus aureus


text: UMCG

characteristic: INSDC first public

text: public

text: Netherlands

text: human

text: Yes

text: rectal swab

text: Staphylococcus aureus

characteristic: organism

text: Staphylococcus aureus

characteristic: title


text: UMCG

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Netherlands

text: human

text: Yes

text: nose/throat swab

text: Staphylococcus aureus

text: Staphylococcus aureus


characteristic: ENA-CHECKLIST

text: UMCG

text: public

characteristic: Submitter Id

text: Netherlands

text: human

text: Yes

text: nose swab

text: Staphylococcus aureus

text: Staphylococcus aureus


characteristic: External Id

text: UMCG

text: public

text: Netherlands

text: human

text: Yes

text: other

text: Staphylococcus aureus

text: Staphylococcus aureus


text: UMCG

text: public

text: Netherlands

text: human

text: Yes

text: pus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: UMCG

text: public

text: Netherlands

text: human

text: Yes

text: wound

text: Staphylococcus aureus

text: Staphylococcus aureus


text: UMCG

text: public

text: Netherlands

text: human

text: Yes

text: sputum

text: Staphylococcus aureus

text: Staphylococcus aureus


text: UMCG

text: public

text: Netherlands

text: human

text: Yes

text: blood culture

text: Staphylococcus aureus

text: Staphylococcus aureus


text: UMCG

text: public

text: Netherlands

text: human

text: Yes

text: perineum

text: Staphylococcus aureus

text: Staphylococcus aureus


text: UMCG

text: public

text: Netherlands

text: human

text: Yes

text: sinus fluid

text: Staphylococcus aureus

text: Staphylococcus aureus


text: UMCG

text: public

text: Netherlands

text: human

text: Yes

text: perianal

text: Staphylococcus aureus

text: Staphylococcus aureus


text: Plasmodium falciparum

characteristic: ArrayExpress-SPECIES

text: SC

text: public

text: malaria parasite P. falciparum

characteristic: common name

text: Plasmodium falciparum

text: Plasmodium falciparum


characteristic: BiologicalReplicate

text: GSC

text: public

text: Spike

characteristic: Organ

characteristic: Species

characteristic: TimePoint (post-inoculation)

characteristic: Treatment

text: Arche

characteristic: WheatCultivar

text: bread wheat

text: total RNA from Triticum aestivum (Arche)

characteristic: description

text: TRIzol protocol + FastDNase treatment

characteristic: mRNA Extraction Method

text: Triticum aestivum

text: Triticum aestivum


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Spike

text: Mock

text: Arche

text: bread wheat

text: TRIzol protocol + FastDNase treatment

text: Triticum aestivum

characteristic: organism

text: Triticum aestivum

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

text: GSC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Spike

characteristic: SRA accession

text: Chinese Spring-MDC_Fg1-48hpi-R1

characteristic: Submitter Id

text: Chinese Spring

text: bread wheat

text: total RNA from Triticum aestivum (Chinese Spring)

text: TRIzol protocol + FastDNase treatment

text: Triticum aestivum

text: Triticum aestivum


characteristic: BiologicalReplicate

text: GSC

text: public

text: Spike

characteristic: Organ

text: Chinese Spring-MDC_Fg1-48hpi-R2

characteristic: Species

characteristic: TimePoint (post-inoculation)

text: Chinese Spring

text: bread wheat

characteristic: common name

text: TRIzol protocol + FastDNase treatment

text: Triticum aestivum

text: Triticum aestivum


characteristic: ENA-FIRST-PUBLIC

text: GSC

text: public

text: Spike

text: Chinese Spring-MDC_Fg1-48hpi-R3

characteristic: Treatment

text: Chinese Spring

characteristic: WheatCultivar

text: bread wheat

characteristic: description

text: TRIzol protocol + FastDNase treatment

characteristic: mRNA Extraction Method

text: Triticum aestivum

text: Triticum aestivum


characteristic: ENA-LAST-UPDATE

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Spike

text: Chinese Spring-MDC_Fg1-72hpi-R1

text: Chinese Spring

text: bread wheat

text: TRIzol protocol + FastDNase treatment

text: Triticum aestivum

characteristic: organism

text: Triticum aestivum

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

text: GSC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Spike

characteristic: SRA accession

text: Chinese Spring-MDC_Fg1-72hpi-R2

text: Chinese Spring

text: bread wheat

text: TRIzol protocol + FastDNase treatment

text: Triticum aestivum

text: Triticum aestivum


characteristic: BiologicalReplicate

text: GSC

text: public

text: Spike

characteristic: Organ

text: Chinese Spring-MDC_Fg1-72hpi-R3

characteristic: Species

characteristic: Submitter Id

characteristic: TimePoint (post-inoculation)

text: Chinese Spring

text: bread wheat

text: TRIzol protocol + FastDNase treatment

text: Triticum aestivum

text: Triticum aestivum


characteristic: ENA-FIRST-PUBLIC

text: GSC

text: public

text: Spike

text: Chinese Spring-MDC_Fg1-96hpi-R1

characteristic: Treatment

text: Chinese Spring

characteristic: WheatCultivar

text: bread wheat

characteristic: common name

characteristic: description

text: TRIzol protocol + FastDNase treatment

text: Triticum aestivum

text: Triticum aestivum


characteristic: ENA-LAST-UPDATE

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Spike

text: Chinese Spring-MDC_Fg1-96hpi-R2

text: Chinese Spring

text: bread wheat

text: TRIzol protocol + FastDNase treatment

characteristic: mRNA Extraction Method

text: Triticum aestivum

characteristic: organism

text: Triticum aestivum


characteristic: ENA-CHECKLIST

characteristic: External Id

text: GSC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Spike

text: Chinese Spring-MDC_Fg1-96hpi-R3

text: Chinese Spring

text: bread wheat

text: TRIzol protocol + FastDNase treatment

text: Triticum aestivum

text: Triticum aestivum

characteristic: title


characteristic: BiologicalReplicate

text: GSC

text: public

text: Spike

characteristic: Organ

characteristic: SRA accession

text: Chinese Spring-Mock-48hpi-R1

characteristic: Species

characteristic: Submitter Id

text: Mock

text: Chinese Spring

text: bread wheat

text: TRIzol protocol + FastDNase treatment

text: Triticum aestivum

text: Triticum aestivum


characteristic: ENA-FIRST-PUBLIC

text: GSC

text: public

text: Spike

text: Chinese Spring-Mock-48hpi-R2

characteristic: TimePoint (post-inoculation)

text: Mock

characteristic: Treatment

text: Chinese Spring

characteristic: WheatCultivar

text: bread wheat

characteristic: common name

text: TRIzol protocol + FastDNase treatment

text: Triticum aestivum

text: Triticum aestivum


characteristic: ENA-LAST-UPDATE

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Spike

text: Chinese Spring-Mock-48hpi-R3

text: Mock

text: Chinese Spring

text: bread wheat

characteristic: description

text: TRIzol protocol + FastDNase treatment

characteristic: mRNA Extraction Method

text: Triticum aestivum

text: Triticum aestivum


characteristic: ENA-CHECKLIST

characteristic: External Id

text: GSC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Spike

text: Chinese Spring-Mock-72hpi-R1

text: Mock

text: Chinese Spring

text: bread wheat

text: TRIzol protocol + FastDNase treatment

text: Triticum aestivum

characteristic: organism

text: Triticum aestivum


characteristic: BiologicalReplicate

text: GSC

text: public

text: Spike

characteristic: Organ

characteristic: SRA accession

text: Chinese Spring-Mock-72hpi-R2

characteristic: Species

text: Mock

text: Chinese Spring

text: bread wheat

text: TRIzol protocol + FastDNase treatment

text: Triticum aestivum

text: Triticum aestivum

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: GSC

text: public

text: Spike

text: Chinese Spring-Mock-72hpi-R3

characteristic: Submitter Id

characteristic: TimePoint (post-inoculation)

text: Mock

characteristic: Treatment

text: Chinese Spring

characteristic: WheatCultivar

text: bread wheat

text: TRIzol protocol + FastDNase treatment

text: Triticum aestivum

text: Triticum aestivum


characteristic: ENA-LAST-UPDATE

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Spike

text: Chinese Spring-Mock-96hpi-R1

text: Mock

text: Chinese Spring

text: bread wheat

characteristic: common name

characteristic: description

text: TRIzol protocol + FastDNase treatment

text: Triticum aestivum

text: Triticum aestivum


characteristic: ENA-CHECKLIST

characteristic: External Id

text: GSC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Spike

text: Chinese Spring-Mock-96hpi-R2

text: Mock

text: Chinese Spring

text: bread wheat

text: TRIzol protocol + FastDNase treatment

characteristic: mRNA Extraction Method

text: Triticum aestivum

text: Triticum aestivum


characteristic: BiologicalReplicate

text: GSC

text: public

text: Spike

characteristic: Organ

characteristic: SRA accession

text: Chinese Spring-Mock-96hpi-R3

characteristic: Species

text: Mock

text: Chinese Spring

text: bread wheat

text: TRIzol protocol + FastDNase treatment

text: Triticum aestivum

characteristic: organism

text: Triticum aestivum


characteristic: ENA-FIRST-PUBLIC

text: GSC

text: public

text: Spike

characteristic: Submitter Id

characteristic: TimePoint (post-inoculation)

characteristic: Treatment

text: Courtot

text: bread wheat

text: total RNA from Triticum aestivum (Courtot)

text: TRIzol protocol + FastDNase treatment

text: Triticum aestivum

text: Triticum aestivum

characteristic: title


characteristic: ENA-LAST-UPDATE

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Spike

text: Mock

text: Courtot

characteristic: WheatCultivar

text: bread wheat

characteristic: common name

text: TRIzol protocol + FastDNase treatment

text: Triticum aestivum

text: Triticum aestivum


characteristic: ENA-CHECKLIST

characteristic: External Id

text: GSC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Spike

text: Recital

text: bread wheat

text: total RNA from Triticum aestivum (Recital)

characteristic: description

text: TRIzol protocol + FastDNase treatment

text: Triticum aestivum

text: Triticum aestivum


characteristic: BiologicalReplicate

text: GSC

text: public

text: Spike

characteristic: Organ

characteristic: SRA accession

characteristic: Species

text: Mock

text: Recital

text: bread wheat

text: TRIzol protocol + FastDNase treatment

characteristic: mRNA Extraction Method

text: Triticum aestivum

text: Triticum aestivum


characteristic: ENA-FIRST-PUBLIC

text: GSC

text: public

text: Spike

characteristic: Submitter Id

characteristic: TimePoint (post-inoculation)

characteristic: Treatment

text: Renan

text: bread wheat

text: total RNA from Triticum aestivum (Renan)

text: TRIzol protocol + FastDNase treatment

text: Triticum aestivum

characteristic: organism

text: Triticum aestivum


characteristic: ENA-LAST-UPDATE

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Spike

text: Mock

text: Renan

characteristic: WheatCultivar

text: bread wheat

text: TRIzol protocol + FastDNase treatment

text: Triticum aestivum

text: Triticum aestivum

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

text: MMRI

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: house mouse

characteristic: common name

text: VIP+ cells obtained and pooled from multiple neonate mouse hippocampus samples

text: Mus musculus

text: Mus musculus

text: Long-read nanopore sequencing of mouse brain cell populations


text: MMRI

text: public

characteristic: SRA accession

text: house mouse

text: VIP- cells obtained and pooled from multiple neonate mouse hippocampus samples

characteristic: description

text: Mus musculus

text: Mus musculus


characteristic: ENA-FIRST-PUBLIC

text: MMRI

text: public

text: house mouse

text: VIP-,Tub+ cells obtained and pooled from multiple neonate mouse hippocampus samples

text: Mus musculus

text: Mus musculus


text: MMRI

text: public

characteristic: Submitter Id

text: house mouse

text: VIP-,Tub- cells obtained and pooled from multiple neonate mouse hippocampus samples

text: Mus musculus

text: Mus musculus


text: MMRI

text: public

text: house mouse

text: Additional PV+ cells obtained and pooled from multiple neonate mouse hippocampus samples

text: Mus musculus

text: Mus musculus


text: MMRI

text: public

text: house mouse

text: Additional PV- cells obtained and pooled from multiple neonate mouse hippocampus samples

text: Mus musculus

text: Mus musculus


text: MMRI

text: public

text: house mouse

text: PV-,Tub+ cells obtained and pooled from multiple neonate mouse hippocampus samples

text: Mus musculus

text: Mus musculus


text: MMRI

text: public

text: house mouse

text: PV-,Tub- cells obtained and pooled from multiple neonate mouse hippocampus samples

text: Mus musculus

text: Mus musculus