text: Wellcome Sanger Institute

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: not collected

text: human

characteristic: common name

text: L. monocytogenes  infected

text: not collected

text: Lm infected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: placenta

characteristic: organism part

text: scRNAseq

text: Homo sapiens

text: Unknown

text: EGD-cGFP

characteristic: title


status: PUBLIC

characteristic: ENA first public

tag: attribute

characteristic: ENA-CHECKLIST

text: Wellcome Sanger Institute

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: ArrayExpress

text: not collected

characteristic: collection date

text: human

text: Protocols: All tissue samples were obtained with written informed consent from all participants in accordance with the guidelines in the Declaration of Helsinki 2000. Placental samples were obtained from elective terminations from The MRC and Wellcome-funded Human Developmental Biology Resource (HDBR, http:// www.hdbr.org), with appropriate maternal written consent and approval from the Fulham Research Ethics Committee (REC reference 18/LO/0822). The HDBR is regulated by the UK Human Tissue Authority (HTA: www.hta.gov.uk) and operates in accordance with the relevant HTA Codes of Practice.  Explants were treated with Corning® Cell recovery solution (Corning, CLS354253) for 15 minutes on ice to remove Matrigel® remnants. The plate was shaken every 5 minutes to dislodge the explant from the membrane. The tissue was transferred to a 50mL Facon tube with ice-cold PBS and washed by inverting, then transferred to a Petri dish with fresh ice-cold PBS, and the villi were gently scraped using forceps and scalpel. Using a Pasteur pipette with a cut tip, all of the tissue was transferred to a Falcon tube with 40 mL of pre-warmed 0.2% trypsin-EDTA and sealed with parafilm. Digestion was performed at 37˚C on a rotator with gentle rotation at 30 rpm for 7 minutes and stopped with 2mL of PBS. The tissue was filtered using a 100µm strainer and the strainer was washed with 5-10 mL of RT PBS (the tissue on the filter was kept for the second step of digestion). The filtrate was centrifuged at 200g for 5 minutes at RT. The supernatant was discarded and the pellet kept on ice (digest 1). The undigested tissue was retrieved from the filter by forceps and placed in a 50 mL Falcon tube containing 12.5mL of prewarmed 1mg/ml Collagenase V (Sigma-Aldrich, C9263) and 40µg/mL DNase I (Roche, 10104159001) in PBS. The tube was sealed with Parafilm and transferred to a tube rotator in the incubator. The tissue was digested for 30 minutes with slow rotation at 30 rpm. The cell suspension was filtered through a 100µm filter and the filter was washed with 5mL of RT PBS. The filter with any undigested tissue was discarded. Cells were pelleted at 200g for 5 minutes at RT (digest 2). Cells from digest 1 and digest 2 were pooled and washed with PBS with 20µg/mL of DNase I (DNase I buffer). Cell suspension was centrifuged at 200g for 5 minutes at RT, the pellet resuspended in 1mL of DNase I buffer and kept on ice. Cells were counted using a haemocytometer.   To remove erythrocytes and debris, a Pancoll gradient was used. The cell suspension was topped up to 10mL with DNase I and very slowly added on top of 5mL of Human Pancoll (PAN-Biotech, P04-60500) in a 15mL Falcon tube. The suspension was centrifuged at 600g for 20 minutes at RT, with using acceleration=0, break=1 settings on the centrifuge. Cells were collected from the interface using a Pasteur pipette. The cells were washed with DNase I buffer and centrifuged at 200g for 5 minutes at RT twice. Cells were passed through a 35-40µm filter and counted (bulk cells). 1 million of these bulk cells were collected for flow cytometry assessment (see Flow cytometry section).  To enrich the final cell suspension for the CD45+ population, 1 million of bulk cells were separated and kept on ice and the rest was submitted to the MACS-based enrichment following the CD45 MicroBeads protocol (product code). The cells were centrifuged at 200g for 5 minutes at RT and the pellet resuspended in DNase I buffer and counted (CD45 cells). Bulk cells and CD45 cells were mixed in 1:1 ratio in PBS/0.04% BSA prior to loading of the 10x Genomics Chromium inlet.   Three independent placental tissue regions per donor were excised and processed to single cell suspension. For hashtag antibody staining, 500,000 cells from bulk and CD45+ enriched fraction were individually re-suspended in Cell Staining Buffer (420201, Biolegend) and blocked with 5 µL Human TruStain FcX™ (422301, Biolegend) for 10 minutes at 4 °C. Each of the cell suspensions originating from different placental tissue regions were hashtagged with different TotalSeq-B™ antibodies for 30 minutes at 4 °C. 1 µg of the following TotalSeq-B™ antibodies were used; TotalSeq™-B0253 anti-human Hashtag 3 Antibody (394635, Biolegend), TotalSeq™-B0254 anti-human Hashtag 4 Antibody (394637, Biolegend) and TotalSeq™-B0255 anti-human Hashtag 5 Antibody (394639, Biolegend). Antibody-tagged cells were washed three times with 3 ml of Cell Staining Buffer using centrifugation speed of 500 x g for 5 minutes at 4°C. Bulk cells and CD45 cells were mixed in 1:1 ratio then filtered through a 40 uM cell strainer. Cells were counted and diluted to desired loading concentration to recover between ~4000 cells. Hashtagged cells were pooled at equal cell number ratio (targeted for a total of ~12000 cells) right before loading of the 10x Genomics Chromium inlet.  Cells and nuclei were loaded into the 10x Genomics Chromium inlet according to the manufacturer’s instructions. The Chromium NEXT GEM Single Cell 3′ Library v3.1 chemistry Dual index (10x Genomics) was used and libraries were prepared according to manufacturer’s instructions aiming to recover between 2,000 and 25,000 cells per reaction. For cell hashing workflow, Chromium NEXT GEM Single Cell 3′ Library v3.1 with Feature Barcoding technology for Cell Surface Protein was used according to the manufacturer’s protocol (10x Genomics).

characteristic: description

characteristic: developmental stage

characteristic: disease

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

characteristic: infect

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: placenta

text: scRNAseq

characteristic: protocol

text: Homo sapiens

text: Unknown

characteristic: sex

characteristic: strain


text: Wellcome Sanger Institute

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: not collected

text: human

characteristic: common name

text: not collected

text: Hrv141, Hrv142

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: placenta

characteristic: organism part

text: scRNAseq

text: Homo sapiens

text: Unknown

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Wellcome Sanger Institute

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: ArrayExpress

text: not collected

characteristic: collection date

text: human

characteristic: description

text: control/none

text: not collected

text: control/none

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: placenta

text: scRNAseq

text: Homo sapiens

text: Unknown


text: Wellcome Sanger Institute

text: public

text: ArrayExpress

text: not collected

text: human

characteristic: developmental stage

text: P. falciparum infected

characteristic: disease

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: Pf infected

characteristic: infect

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: placenta

text: scRNAseq

text: Homo sapiens

text: Unknown

text: 2D9 NF54-eGFP


text: Wellcome Sanger Institute

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: not collected

text: malaria parasite P. falciparum

text: Protocols: All tissue samples were obtained with written informed consent from all participants in accordance with the guidelines in the Declaration of Helsinki 2000. Placental samples were obtained from elective terminations from The MRC and Wellcome-funded Human Developmental Biology Resource (HDBR, http:// www.hdbr.org), with appropriate maternal written consent and approval from the Fulham Research Ethics Committee (REC reference 18/LO/0822). The HDBR is regulated by the UK Human Tissue Authority (HTA: www.hta.gov.uk) and operates in accordance with the relevant HTA Codes of Practice.  Infected placental tissue explant with 2D9 PfNF54eGFP were washed on a rotating platform for five times with 1xPBS to remove unbound parasites, which was designated as ‘pf_nb’. The washed placental tissue explants were then cut into smaller pieces, followed by another gentle washing with 50 mM EDTA (15575020, Invitrogen) on a rotating platform for 1 hr, as previously described88. The EDTA washed fraction, containing potentially cytoadherence population, was filtered through 40µM cell strainer to remove placental tissue pieces. The filtered fraction containing mostly bound parasites, designated as ‘pf_b’, was centrifuged at 1500 rpm and washed five times with 1xPBS. 2D9 PfNF54eGFP used for infection assay was also cultured in parallel in the same batch of TOM media, but without the exposure to placental tissue explants. This batch of culture is designated as ‘pf_iv’ which was also harvested and washed 5 times with 1xPBS. All three parasite fractions, ‘pf_b’, ‘pf_nb’ and ‘pf_iv’ were then resuspended in PBS/0.04% BSA at 3% hematocrit for flow cytometry assessment and loading of the 10x Genomics Chromium inlet. Three independent placental tissue regions per donor were excised and processed to single cell suspension. For hashtag antibody staining, 500,000 cells from bulk and CD45+ enriched fraction were individually re-suspended in Cell Staining Buffer (420201, Biolegend) and blocked with 5 µL Human TruStain FcX™ (422301, Biolegend) for 10 minutes at 4 °C. Each of the cell suspensions originating from different placental tissue regions were hashtagged with different TotalSeq-B™ antibodies for 30 minutes at 4 °C. 1 µg of the following TotalSeq-B™ antibodies were used; TotalSeq™-B0253 anti-human Hashtag 3 Antibody (394635, Biolegend), TotalSeq™-B0254 anti-human Hashtag 4 Antibody (394637, Biolegend) and TotalSeq™-B0255 anti-human Hashtag 5 Antibody (394639, Biolegend). Antibody-tagged cells were washed three times with 3 ml of Cell Staining Buffer using centrifugation speed of 500 x g for 5 minutes at 4°C. Bulk cells and CD45 cells were mixed in 1:1 ratio then filtered through a 40 uM cell strainer. Cells were counted and diluted to desired loading concentration to recover between ~4000 cells. Hashtagged cells were pooled at equal cell number ratio (targeted for a total of ~12000 cells) right before loading of the 10x Genomics Chromium inlet.  Cells and nuclei were loaded into the 10x Genomics Chromium inlet according to the manufacturer’s instructions. The Chromium NEXT GEM Single Cell 3′ Library v3.1 chemistry Dual index (10x Genomics) was used and libraries were prepared according to manufacturer’s instructions aiming to recover between 2,000 and 25,000 cells per reaction. For cell hashing workflow, Chromium NEXT GEM Single Cell 3′ Library v3.1 with Feature Barcoding technology for Cell Surface Protein was used according to the manufacturer’s protocol (10x Genomics).

text: not collected

text: not applicable

text: Plasmodium falciparum

characteristic: organism

text: Plasmodium falciparum

text: scRNAseq

characteristic: protocol

text: Plasmodium falciparum

characteristic: sex

text: 2D9 NF54-eGFP

characteristic: strain


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Wellcome Sanger Institute

text: public

text: ArrayExpress

characteristic: broker name

text: not collected

text: human

characteristic: common name

text: control/ none

text: not collected

text: Hrv171, Hrv172

text: control/ none

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: placenta

characteristic: organism part

text: scRNAseq

text: Homo sapiens

text: Unknown

characteristic: title


text: Wellcome Sanger Institute

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: ArrayExpress

text: not collected

characteristic: collection date

text: human

characteristic: description

characteristic: developmental stage

text: T. gondii infected

characteristic: disease

text: not collected

text: Hrv171, Hrv172

text: Tg infected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: placenta

text: scRNAseq

text: Homo sapiens

text: Unknown

text: RH-HX


text: Wellcome Sanger Institute

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: not collected

text: human

text: Protocols: All tissue samples were obtained with written informed consent from all participants in accordance with the guidelines in the Declaration of Helsinki 2000. Placental samples were obtained from elective terminations from The MRC and Wellcome-funded Human Developmental Biology Resource (HDBR, http:// www.hdbr.org), with appropriate maternal written consent and approval from the Fulham Research Ethics Committee (REC reference 18/LO/0822). The HDBR is regulated by the UK Human Tissue Authority (HTA: www.hta.gov.uk) and operates in accordance with the relevant HTA Codes of Practice.  Three independent placental tissue regions per donor were excised and processed to single cell suspension. For hashtag antibody staining, 500,000 cells from bulk and CD45+ enriched fraction were individually re-suspended in Cell Staining Buffer (420201, Biolegend) and blocked with 5 µL Human TruStain FcX™ (422301, Biolegend) for 10 minutes at 4 °C. Each of the cell suspensions originating from different placental tissue regions were hashtagged with different TotalSeq-B™ antibodies for 30 minutes at 4 °C. 1 µg of the following TotalSeq-B™ antibodies were used; TotalSeq™-B0253 anti-human Hashtag 3 Antibody (394635, Biolegend), TotalSeq™-B0254 anti-human Hashtag 4 Antibody (394637, Biolegend) and TotalSeq™-B0255 anti-human Hashtag 5 Antibody (394639, Biolegend). Antibody-tagged cells were washed three times with 3 ml of Cell Staining Buffer using centrifugation speed of 500 x g for 5 minutes at 4°C. Bulk cells and CD45 cells were mixed in 1:1 ratio then filtered through a 40 uM cell strainer. Cells were counted and diluted to desired loading concentration to recover between ~4000 cells. Hashtagged cells were pooled at equal cell number ratio (targeted for a total of ~12000 cells) right before loading of the 10x Genomics Chromium inlet.  Cells and nuclei were loaded into the 10x Genomics Chromium inlet according to the manufacturer’s instructions. The Chromium NEXT GEM Single Cell 3′ Library v3.1 chemistry Dual index (10x Genomics) was used and libraries were prepared according to manufacturer’s instructions aiming to recover between 2,000 and 25,000 cells per reaction. For cell hashing workflow, Chromium NEXT GEM Single Cell 3′ Library v3.1 with Feature Barcoding technology for Cell Surface Protein was used according to the manufacturer’s protocol (10x Genomics).

text: control/ none

text: not collected

characteristic: geographic location (country and/or sea)

text: Hrv171, Hrv172

characteristic: individual

text: control/ none

characteristic: infect

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: placenta

text: scRNAseq

text: Homo sapiens

text: Unknown


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Wellcome Sanger Institute

text: public

text: ArrayExpress

text: not collected

text: human

text: T. gondii infected

text: not collected

text: Hrv171, Hrv172

text: Tg infected

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: placenta

text: scRNAseq

characteristic: protocol

text: Homo sapiens

text: Unknown

characteristic: sex

text: RH-HX

characteristic: strain


text: Wellcome Sanger Institute

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: ArrayExpress

characteristic: broker name

text: not collected

text: human

characteristic: common name

text: Protocols: All tissue samples were obtained with written informed consent from all participants in accordance with the guidelines in the Declaration of Helsinki 2000. Placental samples were obtained from elective terminations from The MRC and Wellcome-funded Human Developmental Biology Resource (HDBR, http:// www.hdbr.org), with appropriate maternal written consent and approval from the Fulham Research Ethics Committee (REC reference 18/LO/0822). The HDBR is regulated by the UK Human Tissue Authority (HTA: www.hta.gov.uk) and operates in accordance with the relevant HTA Codes of Practice.  Single-nuclei suspensions were isolated either from fresh frozen placental explants, trophoblast organoids or OCT frozen tissue sections (8 x 50 μm cryosections) using a previously described protocol by Krishnaswamiet al, 2016. Isolated nuclei were stained with Trypan blue and counted with a haemocytometer. Nuclei suspension was then resuspended in PBS/1% BSA diluted to targeted final concentration to recover between ~5,000-6000 nuclei per sample prior to loading prior to loading of the 10x Genomics Chromium inlet.  Three independent placental tissue regions per donor were excised and processed to single cell suspension. For hashtag antibody staining, 500,000 cells from bulk and CD45+ enriched fraction were individually re-suspended in Cell Staining Buffer (420201, Biolegend) and blocked with 5 µL Human TruStain FcX™ (422301, Biolegend) for 10 minutes at 4 °C. Each of the cell suspensions originating from different placental tissue regions were hashtagged with different TotalSeq-B™ antibodies for 30 minutes at 4 °C. 1 µg of the following TotalSeq-B™ antibodies were used; TotalSeq™-B0253 anti-human Hashtag 3 Antibody (394635, Biolegend), TotalSeq™-B0254 anti-human Hashtag 4 Antibody (394637, Biolegend) and TotalSeq™-B0255 anti-human Hashtag 5 Antibody (394639, Biolegend). Antibody-tagged cells were washed three times with 3 ml of Cell Staining Buffer using centrifugation speed of 500 x g for 5 minutes at 4°C. Bulk cells and CD45 cells were mixed in 1:1 ratio then filtered through a 40 uM cell strainer. Cells were counted and diluted to desired loading concentration to recover between ~4000 cells. Hashtagged cells were pooled at equal cell number ratio (targeted for a total of ~12000 cells) right before loading of the 10x Genomics Chromium inlet.  Cells and nuclei were loaded into the 10x Genomics Chromium inlet according to the manufacturer’s instructions. The Chromium NEXT GEM Single Cell 3′ Library v3.1 chemistry Dual index (10x Genomics) was used and libraries were prepared according to manufacturer’s instructions aiming to recover between 2,000 and 25,000 cells per reaction. For cell hashing workflow, Chromium NEXT GEM Single Cell 3′ Library v3.1 with Feature Barcoding technology for Cell Surface Protein was used according to the manufacturer’s protocol (10x Genomics).

text: control/ none

text: not collected

text: Hrv168, Hrv170

text: control/ none

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: placenta

characteristic: organism part

text: snRNAseq

text: Homo sapiens

text: Unknown

characteristic: title


text: Wellcome Sanger Institute

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: not collected

characteristic: collection date

text: human

characteristic: description

characteristic: developmental stage

text: P. falciparum infected

characteristic: disease

text: not collected

text: Hrv168, Hrv170

text: Pf infected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: placenta

text: snRNAseq

text: Homo sapiens

text: Unknown

text: 2D9 NF54-eGFP


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Wellcome Sanger Institute

text: public

text: ArrayExpress

text: not collected

text: human

text: control/ none

text: not collected

characteristic: geographic location (country and/or sea)

text: Hrv225, Hrv232

characteristic: individual

text: control/ none

characteristic: infect

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: placenta

text: scRNAseq

text: Homo sapiens

text: Unknown


text: Wellcome Sanger Institute

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: ArrayExpress

text: not collected

text: human

text: P. falciparum infected

text: not collected

text: Hrv225, Hrv232

text: Pf infected

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: placenta

text: scRNAseq

characteristic: protocol

text: Homo sapiens

text: Unknown

characteristic: sex

text: 2D9 NF54-eGFP

characteristic: strain


text: Wellcome Sanger Institute

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: not collected

text: human

characteristic: common name

text: control/ none

text: not collected

text: control/ none

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: placenta

characteristic: organism part

text: scRNAseq

text: Homo sapiens

text: Unknown

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Wellcome Sanger Institute

text: public

text: ArrayExpress

text: not collected

characteristic: collection date

text: human

characteristic: description

characteristic: developmental stage

text: P. falciparum infected

characteristic: disease

text: not collected

text: Pf infected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: placenta

text: scRNAseq

text: Homo sapiens

text: Unknown

text: 2D9 NF54-eGFP


text: Wellcome Sanger Institute

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: ArrayExpress

text: not collected

text: human

text: control/ none

text: not collected

characteristic: geographic location (country and/or sea)

text: Hrv241, Hrv242

characteristic: individual

text: control/ none

characteristic: infect

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: placenta

text: scRNAseq

text: Homo sapiens

text: Unknown


text: Wellcome Sanger Institute

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: not collected

text: human

text: T. gondii infected

text: not collected

text: Hrv241, Hrv242

text: Tg infected

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: placenta

text: scRNAseq

characteristic: protocol

text: Homo sapiens

text: Unknown

characteristic: sex

text: RH-HX

characteristic: strain


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: CDA

text: public

text: not provided

text: bread wheat

characteristic: common name

characteristic: cultivar

text: Total RNA extracted from leaf tissue of Triticum aestivum, Wild Type_SrA_WT

text: not provided

text: Triticum aestivum

text: Triticum aestivum

text: Leaf

text: Zahir-1644 Wheat Leaf RNA_1

characteristic: title


text: CDA

characteristic: INSDC center name

text: public

text: not provided

characteristic: collection date

text: bread wheat

text: Total RNA extracted from leaf tissue of Triticum aestivum, Mutant_SrA_1101a

characteristic: description

text: not provided

text: Triticum aestivum

text: Triticum aestivum

text: Leaf

text: Zahir-1644 Wheat Leaf RNA_8


text: CDA

text: public

characteristic: INSDC status

text: not provided

text: bread wheat

text: Total RNA extracted from leaf tissue of Triticum aestivum, Mutant_SrA_200h

text: not provided

characteristic: geographic location (country and/or sea)

text: Triticum aestivum

text: Triticum aestivum

text: Leaf

text: Zahir-1644 Wheat Leaf RNA_10


text: CDA

text: public

text: not provided

text: bread wheat

text: Total RNA extracted from leaf tissue of Triticum aestivum, Mutant_SrA_353g

text: not provided

text: Triticum aestivum

text: Triticum aestivum

text: Leaf

text: Zahir-1644 Wheat Leaf RNA_11


text: CDA

text: public

text: not provided

text: bread wheat

text: Total RNA extracted from leaf tissue of Triticum aestivum, Mutant_SrA_734e

text: not provided

text: Triticum aestivum

text: Triticum aestivum

text: Leaf

text: Zahir-1644 Wheat Leaf RNA_12


text: CDA

text: public

text: not provided

text: bread wheat

text: Total RNA extracted from leaf tissue of Triticum aestivum, Mutant_SrA_743a

text: not provided

text: Triticum aestivum

text: Triticum aestivum

text: Leaf

text: Zahir-1644 Wheat Leaf RNA_13


text: CDA

text: public

text: not provided

text: bread wheat

text: Total RNA extracted from leaf tissue of Triticum aestivum, Mutant_SrA_896d

text: not provided

text: Triticum aestivum

text: Triticum aestivum

text: Leaf

text: Zahir-1644 Wheat Leaf RNA_14


text: CDA

text: public

text: not provided

text: bread wheat

text: Total RNA extracted from leaf tissue of Triticum aestivum, Mutant_SrA_905c

text: not provided

text: Triticum aestivum

text: Triticum aestivum

text: Leaf

text: Zahir-1644 Wheat Leaf RNA_15


text: Pusan National University

text: public

text: Saliva

characteristic: broad-scale environmental context

text: Saliva

text: Saliva

characteristic: environmental medium

text: South Korea

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Saliva

characteristic: local environmental context

text: clinical metagenome

characteristic: project name

text: clinical metagenome


text: UofG

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Skin lesion

text: USA

text: diseased

characteristic: host health state

text: Megaptera novaeangliae

characteristic: host scientific name

characteristic: isolate

text: Humpback whale carcass

text: Erysipelothrix rhusiopathiae

characteristic: organism

text: Erysipelothrix rhusiopathiae

text: E. rhusiopathiae isolated from humpback whale carcass


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: UofG

characteristic: INSDC center name

text: public

text: Lung

text: USA

text: diseased

text: Delphinapterus leucas

text: Belgua whale carcass

text: Erysipelothrix rhusiopathiae

text: Erysipelothrix rhusiopathiae

text: E. rhusiopathiae isolated from beluga whale carcass

characteristic: title


text: UofG

text: public

characteristic: INSDC status

characteristic: collection date

text: Mammary gland

characteristic: description

text: USA

characteristic: geographic location (country and/or sea)

text: diseased

text: Delphinapterus leucas

text: Belgua whale carcass

text: Erysipelothrix rhusiopathiae

text: Erysipelothrix rhusiopathiae


text: UofG

text: public

text: Rectal swab

text: USA

text: diseased

text: Delphinapterus leucas

text: Belgua whale carcass

text: Erysipelothrix rhusiopathiae

text: Erysipelothrix rhusiopathiae


text: UofG

text: public

text: Heart - aortic valve

text: USA

text: diseased

text: Delphinapterus leucas

text: Belgua whale carcass

text: Erysipelothrix rhusiopathiae

text: Erysipelothrix rhusiopathiae


text: UofG

text: public

text: Tonsil

text: USA

text: diseased

text: Delphinapterus leucas

text: Belgua whale carcass

text: Erysipelothrix rhusiopathiae

text: Erysipelothrix rhusiopathiae


text: UofG

text: public

text: Pericardium

text: USA

text: diseased

text: Enhydra lutris

text: Sea otter carcass

text: Erysipelothrix rhusiopathiae

text: Erysipelothrix rhusiopathiae

text: E. rhusiopathiae isolated from sea otter carcass


text: CENTER FOR EVOLUTIONARY HOLOGENOMICS

text: public

text: Atlantic salmon

characteristic: common name

text: Host Transcriptomics

text: Norway

text: Salmo salar

text: Salmo salar


text: Centre for Translational Microbiome Research (CTMR)

text: public

text: vagina

characteristic: broad-scale environmental context

text: Vaginal swabs of dysplasia samples

text: vaginal swab

characteristic: environmental medium

text: Sweden

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mid fornix

characteristic: local environmental context

text: human vaginal metagenome

text: 031 Vamigyn

text: human vaginal metagenome

text: HSIL/cancer


text: public

characteristic: SRA accession

characteristic: Submitter Id

text: vagina

text: Vaginal swabs of dysplasia samples

text: vaginal swab

text: Sweden

text: mid fornix

text: human vaginal metagenome

text: 031 Vamigyn

characteristic: project name

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