text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00279_MIP_EXP_plt5_24032019_well_330_S270

text: human

text: Homo sapiens

text: Homo sapiens


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: WEIZMANN INSTITUTE OF SCIENCE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: MIP-Prod-2-plt-1-well-87-SampleID-DK00279_S83

tag: Namespace:WEIZMANN INSTITUTE OF SCIENCE

characteristic: Submitter Id

text: human

characteristic: common name

text: Homo sapiens

characteristic: organism

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00281_MIP_EXP_plt5_24032019_well_332_S262

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-1-well-89-SampleID-DK00281_S84

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00282_MIP_EXP_plt5_24032019_well_334_S224

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-1-well-90-SampleID-DK00282_S86

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00283_MIP_EXP_plt5_24032019_well_336_S228

text: human

text: Homo sapiens

text: Homo sapiens


text: OXFORD

text: public

unit: week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: intestinal epithelial cell

text: house mouse

text: Protocols: After treatment, organoids were harvested by direct incubation in 350 ul RLT Plus lysis buffer (Qiagen RNeasy Plus Micro kit) in each well for 5 minutes. At least 30 mg of mouse small intestine was collected from a 5 week-old, wild-type, male C57BL/6 mice for optimal cell yield and organoid establishment. The tissue was transferred into a 50 ml conical centrifuge tube with 30 ml of PBS for short-term storage on ice until use. In a cell culture hood, tissues were transferred to a 10 cm Petri dish. Using small scissors, the intestinal section was cut open lengthwise as such that the lumen of the intestine was facing up and was then minced into smaller pieces of ∼2 mm. Pieces were rinsed twice with cold PBS and transferred into a 50 ml conical centrifugee tube filled with 30 mL of 10% FBS/PBS solution. The tube was shaken forcefully serval times and the intestinal pieces left to settle by gravity to the bottom of the tube. The 10% FBS/PBS solution was replaced with 25 ml of HBSS/EDTA solution (1.86 g of dehydrated EDTA (Sigma-Aldrich) and 500 ul of 10M NAOH (Sigma-Aldrich) in a 500 ml HBSS solution without Ca2+ and Mg2+ (Thermo Fisher Scientific)). The tube was incubated in a 37°C water bath for 10 minutes and shaken forcefully several times every 2-3 minutes. The crypts were released into the supernatant by shaking the tube. The supernatants containing the isolated crypts were collected and the procedure was repeated twice. Each fraction was filtered through a 150 µm cell strainer to remove debris and for crypts enrichment and 1ml of each fraction was examined under the microscope to check for culturable crypts (it is common for the first fraction to have minimal crypts isolated). 25 ml of 10% FBS/PBS solution was added to the fraction with higher numbers of isolated crypts and centrifuged at 1200 rpm at 4°C for five minutes. The pellet was washed with 25 ml of 10% FBS/PBS solution and spinned down again. The supernatant was removed carefully and the crypt containing pellets was resuspended in cold Cultrex™ UltiMatrix Reduced Growth Factor (RGF) Basement Membrane Extract (BME) (Bio-Techne). Approximately 250 crypts per 20 ul of BME were seeded in each well of a pre-warmed 48-well plate. The BME was then solidified by a 20-minute incubation in a 37°C and 5% CO2 cell culture incubator and overlaid with 500 µl of complete mouse organoid media (Advanced Dulbecco’s Modified Eagle Medium/F12 (Thermo Fisher) supplemented with GlutaMAX, 10mM HEPES, 100ug/mL Primocin, 1X N-2 supplement, 1X B-27 supplement, 0.1% BSA, 25% R-spondin-1 conditioned medium, 100 ng/mL mouse EGF, 100 ng/mL mouse Noggin, and 3 μM CHIR 99021) with 10 μM Y-27632 (Tocris). 10 µM Y-27632 ameliorate organoids establishment and apoptosis survival. Complete media was subsequently refreshed every two days. Organoids were usually passaged every 5–10 days by a mechanical approach using Gentle Cell Dissociation Reagent (GCDR) (STEMCELL Technologies). The mechanical method is recommended for expansion of organoids and before freezing as it is less harsh. Briefly, medium was aspirated and organoids were incubated with 500 ml GCDR at room temperature for a minimum of 10 minutes. Using a p1000 pipette, the BME domes were scraped off the surface of the plate and transferred with the GCDR into a 15 mL conical centrifuge tube (Multiple wells can be split together into one tube). The gel/GCDR mix were pipetted several times to break up the organoids. For maintenance passage, 10–15 rounds of pipetting are sufficient to dissociate the organoids to fragments that will quickly regenerate complete organoids. The tube was filled with basal medium up to 10 ml, and centrifuged at 1200 rpm for 5 min. The pellets were resuspended in BME and re-seeded at an appropriate ratio. Organoids were biobanked in FBS (Thermo Fisher Scientific) containing 5% DMSO (SigmaAldrich). Organoids were plated on Day 0 and the media was supplemented with 5 μg/ml PMX205 (Tocris #5196) dissolved in 20% ethanol/water on Day 1. Control organoids were provided with media supplemented with an equal amount of vehicle control (20% ethanol/water). Cells were pre-treated for 1 hour before irradiation with either 0 or 9 Gy irradiation. Samples were collected either 24 h or 48 h after irradiation with 9 Gy. RNA was extracted using the Qiagen RNeasy Plus Micro Kit according to the manufacturer protocol. RNA quality and concentration were measured with Agilent Bioanalyzer 2100 (Agilent) and Nanodrop (Thermo Fisher Scientific) respectively. Library construction was performed by Novogene UK. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers. Non-directional libraries were prepared by second strand cDNA synthesis using dTTP and were ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. Libraries were checked for quantification with Qubit and real-time PCR and for size distribution detection with an Agilent Bioanalyzer.

characteristic: description

text: wild type genotype

characteristic: genotype

text: intestinal organoid

characteristic: growth condition

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: small intestine

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: WEIZMANN INSTITUTE OF SCIENCE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: MIP-Prod-2-plt-1-well-91-SampleID-DK00283_S87

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: DK00284_MIP_EXP_plt5_24032019_well_354_S382

characteristic: Submitter Id

text: human

characteristic: common name

text: Homo sapiens

characteristic: organism

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-1-well-92-SampleID-DK00284_S88

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00285_MIP_EXP_plt5_24032019_well_356_S340

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-1-well-93-SampleID-DK00285_S89

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00286_MIP_EXP_plt5_24032019_well_358_S383

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-1-well-94-SampleID-DK00286_S90

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00287_MIP_EXP_plt5_24032019_well_360_S254

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-1-well-95-SampleID-DK00287_S91

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00288_MIP_EXP_plt5_24032019_well_362_S252

text: human

text: Homo sapiens

text: Homo sapiens


E-MTAB-12559:Sample 6

text: public

text: E-MTAB-12559:Sample 6

tag: Namespace:Institute of Immunobiology, Kantonsspital St. Gallen, St. Gallen, Switzerland

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

text: Protocols: Euthanized animals were perfused with 20 ml of PBS before hearts were harvested. Mice were treated i.p. twice per week with 200 µg of 14-D10-2 anti-Gremlin monoclonal antibodies or IgG2b isotype control. For enzymatic dissociation hearts were minced into small pieces and were placed into a six-well dish filled with RPMI 1640 medium containing 2% FCS, 20 mM HEPES (Lonza), 1 mg/ml collagenase D (Sigma), and 25 μg/ml DNase I (Applichem) and incubated at 37°C under continuous stirring. The remaining tissue pieces were mechanically disrupted. Nuclei were isolated from frozen murine hearts and sorted to enrich intact nuclei using a BDFACSMelody. Sorted single nuclei suspensions were emulsified for library generation using the droplet-based 10x Chromium (10x Genomics) system. cDNA library generation was performed following the established commercial protocol for Chromium Single Cell 3’ Reagent Kit (v3 Chemistry).

characteristic: description

text: adult

characteristic: developmental stage

text: myocarditis

characteristic: disease

text: MYH6-specific TCR transgene

characteristic: genotype

text: mouse 6

characteristic: individual

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: heart

text: Mus musculus

text: male

text: BALB/c

text: Sample 6


E-MTAB-12559:Sample 7

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Institute of Immunobiology, Kantonsspital St. Gallen, St. Gallen, Switzerland

text: public

text: E-MTAB-12559:Sample 7

text: ArrayExpress

text: house mouse

text: adult

text: myocarditis

text: MYH6-specific TCR transgene

text: mouse 7

text: not applicable

text: Mus musculus

text: Mus musculus

text: heart

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex

text: BALB/c

characteristic: strain

text: Sample 7

characteristic: title


E-MTAB-12559:Sample 8

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12559:Sample 8

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

characteristic: common name

text: adult

text: myocarditis

text: MYH6-specific TCR transgene

text: mouse 8

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: heart

text: Mus musculus

text: male

text: BALB/c

text: Sample 8


E-MTAB-12559:Sample 4

text: public

text: E-MTAB-12559:Sample 4

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: myocarditis

characteristic: disease

text: MYH6-specific TCR transgene

characteristic: genotype

text: mouse 4

characteristic: individual

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: heart

text: Mus musculus

text: male

text: BALB/c

text: Sample 4


E-MTAB-12559:Sample 9

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-12559:Sample 9

text: ArrayExpress

text: house mouse

text: adult

text: myocarditis

text: MYH6-specific TCR transgene

text: mouse 9

text: not applicable

text: Mus musculus

text: Mus musculus

text: heart

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex

text: BALB/c

characteristic: strain

text: Sample 9

characteristic: title


E-MTAB-12559:Sample 5

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12559:Sample 5

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

characteristic: common name

text: adult

text: myocarditis

text: MYH6-specific TCR transgene

text: mouse 5

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: heart

text: Mus musculus

text: male

text: BALB/c

text: Sample 5


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-1-well-96-SampleID-DK00288_S92

text: human

text: Homo sapiens

text: Homo sapiens


E-MTAB-12559:Sample 1

text: public

text: E-MTAB-12559:Sample 1

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: mouse 1

characteristic: individual

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: heart

text: Mus musculus

text: male

text: BALB/c

text: Sample 1


E-MTAB-12559:Sample 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-12559:Sample 2

text: ArrayExpress

text: house mouse

text: adult

text: normal

text: wild type genotype

text: mouse 2

text: not applicable

text: Mus musculus

text: Mus musculus

text: heart

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: BALB/c

characteristic: strain

text: Sample 2

characteristic: title


E-MTAB-12559:Sample 3

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12559:Sample 3

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

characteristic: common name

text: adult

text: myocarditis

text: MYH6-specific TCR transgene

text: mouse 3

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: heart

text: Mus musculus

text: female

text: BALB/c

text: Sample 3


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00291_MIP_EXP_plt5_24032019_well_366_S250

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-2-well-3-SampleID-DK00291_S122

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00292_MIP_EXP_plt5_24032019_well_368_S272

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-2-well-4-SampleID-DK00292_S133

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00293_MIP_EXP_plt5_24032019_well_18_S373

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-2-well-5-SampleID-DK00293_S144

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00294_MIP_EXP_plt5_24032019_well_20_S182

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-2-well-6-SampleID-DK00294_S155

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00295_MIP_EXP_plt5_24032019_well_22_S234

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-2-well-7-SampleID-DK00295_S166

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00296_MIP_EXP_plt5_24032019_well_24_S146

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-2-well-8-SampleID-DK00296_S176

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00298_MIP_EXP_plt5_24032019_well_28_S126

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-2-well-10-SampleID-DK00298_S93

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00299_MIP_EXP_plt5_24032019_well_30_S48

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-2-well-11-SampleID-DK00299_S94

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00300_MIP_EXP_plt5_24032019_well_32_S142

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-2-well-12-SampleID-DK00300_S95

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00301_MIP_EXP_plt5_24032019_well_50_S342

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-2-well-13-SampleID-DK00301_S96

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00302_MIP_EXP_plt5_24032019_well_52_S54

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-2-well-14-SampleID-DK00302_S97

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00303_MIP_EXP_plt5_24032019_well_54_S282

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-2-well-15-SampleID-DK00303_S98

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00304_MIP_EXP_plt5_24032019_well_56_S185

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-2-well-16-SampleID-DK00304_S99

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00305_MIP_EXP_plt5_24032019_well_58_S138

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-2-well-17-SampleID-DK00305_S100

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00307_MIP_EXP_plt5_24032019_well_62_S85

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-2-well-19-SampleID-DK00307_S101

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00308_MIP_EXP_plt5_24032019_well_64_S84

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-2-well-20-SampleID-DK00308_S102

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00309_MIP_EXP_plt5_24032019_well_82_S367

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-2-well-21-SampleID-DK00309_S103

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00310_MIP_EXP_plt5_24032019_well_84_S3

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-2-well-22-SampleID-DK00310_S104

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00311_MIP_EXP_plt5_24032019_well_86_S303

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-2-well-23-SampleID-DK00311_S105

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00312_MIP_EXP_plt5_24032019_well_88_S162

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-2-well-24-SampleID-DK00312_S106

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00313_MIP_EXP_plt5_24032019_well_90_S101

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-2-well-25-SampleID-DK00313_S107

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00314_MIP_EXP_plt5_24032019_well_92_S147

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-2-well-26-SampleID-DK00314_S108

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00315_MIP_EXP_plt5_24032019_well_94_S38

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-2-well-27-SampleID-DK00315_S109

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00316_MIP_EXP_plt5_24032019_well_96_S46

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-2-well-28-SampleID-DK00316_S110

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00317_MIP_EXP_plt5_24032019_well_114_S372

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-2-well-29-SampleID-DK00317_S111

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

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characteristic: broker name

text: house mouse

text: Protocols: Tumours were excised from radiation-treated mice at 4 hours, 24 hours, 3 days, and 7 days after the completion of each irradiation scheme (15Gy and 3x7Gy) as well as from corresponding untreated/non-irradiated mice and stored in RNAprotect tissue reagent (Qiagen) at -20°C prior to RNA extraction. The AKPT (villinCreER;Apcfl/fl;KrasG12D/+;Trp53fl/fl;TgfbrIfl/fl) organoids were maintained in Advanced Dulbecco’s Modified Eagle Medium/F12 (Thermo Fisher) supplemented with penicillin/streptomycin, 10 mmol/L HEPES, Glutamax, 1x N2 supplement (Thermo Fisher), 1× B27 supplement (Thermo Fisher), 1.25 mmol/L N-acetyl-l-cysteine (Sigma-Aldrich), 50 ng/mL recombinant murine epidermal growth factor (Thermo Fisher) and 100 ng/mL noggin (PeproTech). Organoids were suspended in a phosphate-buffered saline and Matrigel (Corning) mixture (50:50). Organoids were then implanted into male C57BL/6 mice subcutaneously, monitored, and treated two weeks after implantation. Once the subcutaneous tumour volumes reached a volume of 150-200 mm3, mice were given either 15 Gy radiation as a single dose or 3 doses of 7Gy, using the Gulmay Medical RS320 irradiator (300kV, 10mA, 1.81Gy/min). For the fractionated irradiation, tumours received 7Gy of radiation three times, with 24 hours gap between each dose. Tumours were harvested after recieving the single dose of 15Gy or after the final dose of 7Gy at the previously described timepoints (4 hours, 24 hours, 3days, 7days). was extracted using the RNeasy Micro Kit from Qiagen (74004) and DNase treatment was performed using the DNA-free kit from Life Technologies (AM1906). The starting RNA material was quantified using RiboGreen (Invitrogen) on the FLUOstar OPTIMA plate reader (BMG Labtech) and the size profile and integrity analysed on the TapeStation (RNA ScreenTape, Agilent). 3'RNAseq libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD (Lexogen, Austria) and the UMI Second strand synthesis module for QuantSeq FWD (Lexogen, Austria) following the manufacturer's instructions. RNA input varying from 100-1000ng and 14-20 PCR cycles were used. Library quality and quantity were assessed using the TapeStation (Agilent) and Qubit Assay (ThermoFisher) respectively. Individual libraries were normalised and pooled accordingly to multiplex for sequencing.

text: adult

characteristic: developmental stage

text: small intestine carcinoma

characteristic: disease

text: wild type genotype

characteristic: genotype

characteristic: individual

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: small intestine

characteristic: organism part

text: neoplasm

characteristic: sampling site

text: Mus musculus

text: male

characteristic: sex

text: AKPT (villinCreER;Apcfl/fl;KrasG12D/+;Trp53fl/fl;TgfbrIfl/fl) intestinal organoid

characteristic: xenograft


text: WEIZMANN INSTITUTE OF SCIENCE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: MIP-Prod-2-plt-4-well-19-SampleID-DK00499_S282

characteristic: Submitter Id

text: human

characteristic: common name

text: Homo sapiens

characteristic: organism

text: Homo sapiens


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00500_MIP_EXP_plt6_30032019_well_61_S531

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-20-SampleID-DK00500_S283

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00501_MIP_EXP_plt6_30032019_well_63_S626

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-21-SampleID-DK00501_S284

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00502_MIP_EXP_plt6_30032019_well_81_S577

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-22-SampleID-DK00502_S285

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00503_MIP_EXP_plt6_30032019_well_83_S469

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-23-SampleID-DK00503_S286

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00504_MIP_EXP_plt6_30032019_well_85_S729

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-24-SampleID-DK00504_S287

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00505_MIP_EXP_plt6_30032019_well_87_S575

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-25-SampleID-DK00505_S288

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00506_MIP_EXP_plt6_30032019_well_89_S485

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-26-SampleID-DK00506_S289

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00507_MIP_EXP_plt6_30032019_well_91_S642

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-27-SampleID-DK00507_S290

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00508_MIP_EXP_plt6_30032019_well_93_S691

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-28-SampleID-DK00508_S291

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00509_MIP_EXP_plt6_30032019_well_95_S517

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-29-SampleID-DK00509_S292

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00510_MIP_EXP_plt6_30032019_well_113_S515

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-30-SampleID-DK00510_S293

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00511_MIP_EXP_plt6_30032019_well_115_S631

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-31-SampleID-DK00511_S294

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00512_MIP_EXP_plt6_30032019_well_117_S445

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-32-SampleID-DK00512_S295

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00513_MIP_EXP_plt6_30032019_well_119_S734

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-33-SampleID-DK00513_S296

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00514_MIP_EXP_plt6_30032019_well_121_S663

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-34-SampleID-DK00514_S297

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-35-SampleID-DK00515_S298

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00516_MIP_EXP_plt6_30032019_well_125_S555

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-36-SampleID-DK00516_S299

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00517_MIP_EXP_plt6_30032019_well_127_S585

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-37-SampleID-DK00517_S300

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00518_MIP_EXP_plt6_30032019_well_145_S673

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-38-SampleID-DK00518_S301

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00519_MIP_EXP_plt6_30032019_well_147_S655

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-39-SampleID-DK00519_S302

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00520_MIP_EXP_plt6_30032019_well_149_S477

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-40-SampleID-DK00520_S304

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00521_MIP_EXP_plt6_30032019_well_151_S509

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-41-SampleID-DK00521_S305

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-42-SampleID-DK00522_S306

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00523_MIP_EXP_plt6_30032019_well_155_S535

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00524_MIP_EXP_plt6_30032019_well_157_S424

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-44-SampleID-DK00524_S307

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00525_MIP_EXP_plt6_30032019_well_159_S566

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-45-SampleID-DK00525_S308

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00526_MIP_EXP_plt6_30032019_well_177_S656

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-46-SampleID-DK00526_S309

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00527_MIP_EXP_plt6_30032019_well_179_S725

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-47-SampleID-DK00527_S310

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00528_MIP_EXP_plt6_30032019_well_181_S406

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-48-SampleID-DK00528_S311

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00529_MIP_EXP_plt6_30032019_well_183_S672

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-49-SampleID-DK00529_S312

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00530_MIP_EXP_plt6_30032019_well_185_S410

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-50-SampleID-DK00530_S314

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00531_MIP_EXP_plt6_30032019_well_187_S536

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-51-SampleID-DK00531_S315

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00532_MIP_EXP_plt6_30032019_well_189_S633

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-52-SampleID-DK00532_S316

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00533_MIP_EXP_plt6_30032019_well_191_S591

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-53-SampleID-DK00533_S317

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00535_MIP_EXP_plt6_30032019_well_209_S599

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-55-SampleID-DK00535_S318

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00536_MIP_EXP_plt6_30032019_well_211_S519

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-56-SampleID-DK00536_S319

text: human

text: Homo sapiens

text: Homo sapiens


text: IRD-MIVEGEC

text: public

text: IRD

characteristic: collecting institution

text: Christelle Ngou

characteristic: collector name

text: malaria parasite P. falciparum

text: Parasite cell sequencing data from individual C204

characteristic: description

text: Blood stages

text: Cameroon

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: isolate

text: Plasmodium falciparum

text: Plasmodium falciparum

characteristic: title


text: IRD-MIVEGEC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: IRD

text: Christelle Ngou

text: malaria parasite P. falciparum

text: Parasite cell sequencing data from individual C253

text: Blood stages

text: Cameroon

text: not provided

text: Plasmodium falciparum

text: Plasmodium falciparum


text: IRD-MIVEGEC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: IRD

text: Christelle Ngou

text: malaria parasite P. falciparum

text: Parasite cell sequencing data from individual C337

text: Blood stages

text: Cameroon

text: not provided

text: Plasmodium falciparum

text: Plasmodium falciparum


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

characteristic: SRA accession

text: DK00537_MIP_EXP_plt6_30032019_well_213_S514

characteristic: Submitter Id

text: human

text: Homo sapiens

text: Homo sapiens


text: IRD-MIVEGEC

text: public

text: IRD

text: Christelle Ngou

text: malaria parasite P. falciparum

characteristic: common name

text: Parasite cell sequencing data from individual C311

text: Blood stages

text: Cameroon

text: not provided

text: Plasmodium falciparum

characteristic: organism

text: Plasmodium falciparum


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IRD-MIVEGEC

text: public

text: IRD

text: Christelle Ngou

text: malaria parasite P. falciparum

text: Parasite cell sequencing data from individual C228

text: Blood stages

text: Cameroon

text: not provided

text: Plasmodium falciparum

text: Plasmodium falciparum


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IRD-MIVEGEC

text: public

text: IRD

text: Christelle Ngou

text: malaria parasite P. falciparum

text: Parasite cell sequencing data from individual C276

text: Blood stages

text: Cameroon

text: not provided

text: Plasmodium falciparum

text: Plasmodium falciparum


text: IRD-MIVEGEC

text: public

text: IRD

characteristic: collecting institution

text: Christelle Ngou

characteristic: collector name

text: malaria parasite P. falciparum

text: Parasite cell sequencing data from individual C250

text: Blood stages

text: Cameroon

text: not provided

text: Plasmodium falciparum

text: Plasmodium falciparum


text: IRD-MIVEGEC

text: public

text: IRD

text: Christelle Ngou

text: malaria parasite P. falciparum

text: Parasite cell sequencing data from individual C220

characteristic: description

text: Blood stages

text: Cameroon

characteristic: geographic location (country and/or sea)

text: not provided

text: Plasmodium falciparum

text: Plasmodium falciparum


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-57-SampleID-DK00537_S320

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00538_MIP_EXP_plt6_30032019_well_215_S433

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-58-SampleID-DK00538_S321

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00539_MIP_EXP_plt6_30032019_well_217_S389

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-59-SampleID-DK00539_S322

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00540_MIP_EXP_plt6_30032019_well_219_S471

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: MIP-Prod-2-plt-4-well-60-SampleID-DK00540_S324

text: human

text: Homo sapiens

text: Homo sapiens


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

text: DK00541_MIP_EXP_plt6_30032019_well_221_S478

text: human

text: Homo sapiens

text: Homo sapiens


text: IRD-MIVEGEC

text: public

text: IRD

text: Christelle Ngou

text: malaria parasite P. falciparum

text: Parasite cell sequencing data from individual C204

text: Blood stages

text: Cameroon

text: not provided

characteristic: isolate

text: Plasmodium falciparum

text: Plasmodium falciparum

characteristic: title


text: WEIZMANN INSTITUTE OF SCIENCE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: MIP-Prod-2-plt-4-well-61-SampleID-DK00541_S325

text: human

text: Homo sapiens

text: Homo sapiens


text: IRD-MIVEGEC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: IRD

text: Christelle Ngou

text: malaria parasite P. falciparum

text: Parasite cell sequencing data from individual C253

text: Blood stages

text: Cameroon

text: not provided

text: Plasmodium falciparum

text: Plasmodium falciparum


text: WEIZMANN INSTITUTE OF SCIENCE

text: public

characteristic: SRA accession

text: DK00542_MIP_EXP_plt6_30032019_well_223_S707

characteristic: Submitter Id

text: human

text: Homo sapiens

text: Homo sapiens


text: IRD-MIVEGEC

text: public

text: IRD

text: Christelle Ngou

text: malaria parasite P. falciparum

characteristic: common name

text: Parasite cell sequencing data from individual C311

text: Blood stages

text: Cameroon

text: not provided

text: Plasmodium falciparum

characteristic: organism

text: Plasmodium falciparum