characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Dilution; Light condition: IR; Replicate: B; Time: 32h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1191226.5 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 252468.9 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

text: DADA2 version 1.18.0; function: removeBimeraDenovo; method=pooled

characteristic: chimera check software

characteristic: collection date

unit: m

characteristic: depth

text: Treatment: Dilution; Light condition: IR; Replicate: C; Time: 32h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1075659.75 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 276174.9 cells per ml; epifluorescent microscopy

text: aerobic

text: intital denaturation:98degC_3min;denaturation:98degC_10s; annealing:52degC_30s;elongation:72degC_30s;number of cycles:30; final elongation:72degC_5min

text: pufM_uniF: GGNAAYYTNTWYTAYAAY; pufM_uniR: YCCATNGTCCANCKCCARAACCNTTYCA

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

unit: days

text: Centre Algatech, freezere on the coridor

unit: mL

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Lignin; Light condition: Dark; Replicate: A; Time: 31.6h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1519339.09090909 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 279730.8 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Lignin; Light condition: Dark; Replicate: B; Time: 31.6h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1178385.75 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 207427.5 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Lignin; Light condition: Dark; Replicate: C; Time: 31.6h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1515490.71428571 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 234689.4 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Lignin; Light condition: IR; Replicate: A; Time: 31.6h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1306793.25 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 225207 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Lignin; Light condition: IR; Replicate: B; Time: 31.6h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1029517.71428571 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 201501 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Lignin; Light condition: IR; Replicate: C; Time: 31.6h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 934016.4 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 182536.2 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: DADA2 version 1.18.0; function: removeBimeraDenovo; method=pooled

text: Treatment: Control; Light condition: Dark; Replicate: A; Time: 38.25h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 2019619.5 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 447450.75 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Control; Light condition: Dark; Replicate: B; Time: 38.25h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1991304 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 427893.3 cells per ml; epifluorescent microscopy

text: aerobic

text: intital denaturation:98degC_3min;denaturation:98degC_10s; annealing:52degC_30s;elongation:72degC_30s;number of cycles:30; final elongation:72degC_5min

text: pufM_uniF: GGNAAYYTNTWYTAYAAY; pufM_uniR: YCCATNGTCCANCKCCARAACCNTTYCA

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Control; Light condition: Dark; Replicate: C; Time: 38.25h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 2047935 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 467008.2 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

text: Centre Algatech, freezere on the coridor

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Control; Light condition: IR; Replicate: A; Time: 38.25h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 2003157 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 440931.6 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Control; Light condition: IR; Replicate: B; Time: 38.25h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1706832 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 352034.1 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Control; Light condition: IR; Replicate: C; Time: 38.25h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1854994.5 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 396482.85 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Dilution; Light condition: Dark; Replicate: A; Time: 44h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1333462.5 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 278545.5 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

text: DADA2 version 1.18.0; function: removeBimeraDenovo; method=pooled

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Dilution; Light condition: Dark; Replicate: B; Time: 44h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1218389.625 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 265507.2 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Dilution; Light condition: Dark; Replicate: C; Time: 44h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1103316.75 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 252468.9 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Dilution; Light condition: IR; Replicate: A; Time: 44h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1552743 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 309363.3 cells per ml; epifluorescent microscopy

text: aerobic

text: intital denaturation:98degC_3min;denaturation:98degC_10s; annealing:52degC_30s;elongation:72degC_30s;number of cycles:30; final elongation:72degC_5min

text: pufM_uniF: GGNAAYYTNTWYTAYAAY; pufM_uniR: YCCATNGTCCANCKCCARAACCNTTYCA

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Dilution; Light condition: IR; Replicate: B; Time: 44h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1419396.75 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 320031 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

text: Centre Algatech, freezere on the coridor

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Dilution; Light condition: IR; Replicate: C; Time: 44h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1262344.5 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 311733.9 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Lignin; Light condition: Dark; Replicate: A; Time: 43.6h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1309756.5 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 277360.2 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Lignin; Light condition: Dark; Replicate: B; Time: 43.6h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1206635.4 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 245357.1 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: DADA2 version 1.18.0; function: removeBimeraDenovo; method=pooled

text: Treatment: Lignin; Light condition: Dark; Replicate: C; Time: 43.6h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1133443.125 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 225207 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Lignin; Light condition: IR; Replicate: A; Time: 43.6h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1112206.5 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 190833.3 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Lignin; Light condition: IR; Replicate: B; Time: 43.6h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1222834.5 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 193203.9 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Lignin; Light condition: IR; Replicate: C; Time: 43.6h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 485973 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 82971 cells per ml; epifluorescent microscopy

text: aerobic

text: intital denaturation:98degC_3min;denaturation:98degC_10s; annealing:52degC_30s;elongation:72degC_30s;number of cycles:30; final elongation:72degC_5min

text: pufM_uniF: GGNAAYYTNTWYTAYAAY; pufM_uniR: YCCATNGTCCANCKCCARAACCNTTYCA

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Control; Light condition: Dark; Replicate: A; Time: 50.25h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1964211.42857143 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 336625.2 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

text: Centre Algatech, freezere on the coridor

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Control; Light condition: Dark; Replicate: B; Time: 50.25h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 2019751.2 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 318845.7 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Control; Light condition: Dark; Replicate: C; Time: 50.25h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 2515799.25 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 373369.5 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

text: DADA2 version 1.18.0; function: removeBimeraDenovo; method=pooled

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Control; Light condition: IR; Replicate: A; Time: 50.25h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1711911.85714286 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 482417.1 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Control; Light condition: IR; Replicate: B; Time: 50.25h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 2209737.85714286 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 581982.3 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Control; Light condition: IR; Replicate: C; Time: 50.25h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 2408529.6 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 606873.6 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Dilution; Light condition: Dark; Replicate: A; Time: 56h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1672590 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 407743.2 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Dilution; Light condition: Dark; Replicate: B; Time: 56h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1097884.125 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 293954.4 cells per ml; epifluorescent microscopy

text: aerobic

text: intital denaturation:98degC_3min;denaturation:98degC_10s; annealing:52degC_30s;elongation:72degC_30s;number of cycles:30; final elongation:72degC_5min

text: pufM_uniF: GGNAAYYTNTWYTAYAAY; pufM_uniR: YCCATNGTCCANCKCCARAACCNTTYCA

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Dilution; Light condition: Dark; Replicate: C; Time: 56h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1775974.5 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 450414 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

text: Centre Algatech, freezere on the coridor

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Dilution; Light condition: IR; Replicate: A; Time: 56h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1989328.5 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 651915 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: DADA2 version 1.18.0; function: removeBimeraDenovo; method=pooled

text: Treatment: Dilution; Light condition: IR; Replicate: B; Time: 56h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1626824.25 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 462267 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Dilution; Light condition: IR; Replicate: C; Time: 56h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1252467 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 352034.1 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Lignin; Light condition: Dark; Replicate: A; Time: 55.6h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1745777.57142857 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 335439.9 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Lignin; Light condition: Dark; Replicate: B; Time: 55.6h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1406556 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 274989.6 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Lignin; Light condition: Dark; Replicate: C; Time: 55.6h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1676660.72727273 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 337810.5 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Lignin; Light condition: IR; Replicate: A; Time: 55.6h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1151716.5 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 169497.9 cells per ml; epifluorescent microscopy

text: aerobic

text: intital denaturation:98degC_3min;denaturation:98degC_10s; annealing:52degC_30s;elongation:72degC_30s;number of cycles:30; final elongation:72degC_5min

text: pufM_uniF: GGNAAYYTNTWYTAYAAY; pufM_uniR: YCCATNGTCCANCKCCARAACCNTTYCA

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Lignin; Light condition: IR; Replicate: B; Time: 55.6h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1238638.5 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 232318.8 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

text: Centre Algatech, freezere on the coridor

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

text: DADA2 version 1.18.0; function: removeBimeraDenovo; method=pooled

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Lignin; Light condition: IR; Replicate: C; Time: 55.6h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1512442.8 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 256024.8 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Control; Light condition: Dark; Replicate: A; Time: 0h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1014616.8 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 93638.7 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Control; Light condition: Dark; Replicate: B; Time: 0h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 968390.1 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 59265 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Control; Light condition: Dark; Replicate: C; Time: 0h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1107070.2 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 50967.9 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Control; Light condition: IR; Replicate: A; Time: 0h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 855786.6 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 48597.3 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Control; Light condition: IR; Replicate: B; Time: 0h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 813115.8 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 36744.3 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Control; Light condition: IR; Replicate: C; Time: 0h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 824968.8 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 39114.9 cells per ml; epifluorescent microscopy

text: aerobic

text: intital denaturation:98degC_3min;denaturation:98degC_10s; annealing:52degC_30s;elongation:72degC_30s;number of cycles:30; final elongation:72degC_5min

text: pufM_uniF: GGNAAYYTNTWYTAYAAY; pufM_uniR: YCCATNGTCCANCKCCARAACCNTTYCA

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: DADA2 version 1.18.0; function: removeBimeraDenovo; method=pooled

text: Treatment: Dilution; Light condition: Dark; Replicate: A; Time: 0h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 868824.9 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 42670.8 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

text: Centre Algatech, freezere on the coridor

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Dilution; Light condition: Dark; Replicate: B; Time: 0h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 794151 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 58079.7 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Dilution; Light condition: Dark; Replicate: C; Time: 0h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 923348.7 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 56894.4 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Dilution; Light condition: IR; Replicate: A; Time: 0h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1092846.6 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 53338.5 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Dilution; Light condition: IR; Replicate: B; Time: 0h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1085734.8 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 47412 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Dilution; Light condition: IR; Replicate: C; Time: 0h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 904383.9 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 40300.2 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Acetate; Light condition: Dark; Replicate: A; Time: 0h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1051361.1 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 37929.6 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Acetate; Light condition: Dark; Replicate: B; Time: 0h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1245750.3 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 68747.4 cells per ml; epifluorescent microscopy

text: aerobic

text: intital denaturation:98degC_3min;denaturation:98degC_10s; annealing:52degC_30s;elongation:72degC_30s;number of cycles:30; final elongation:72degC_5min

text: pufM_uniF: GGNAAYYTNTWYTAYAAY; pufM_uniR: YCCATNGTCCANCKCCARAACCNTTYCA

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: DADA2 version 1.18.0; function: removeBimeraDenovo; method=pooled

text: Treatment: Acetate; Light condition: Dark; Replicate: C; Time: 0h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 964834.2 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 43856.1 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

text: Centre Algatech, freezere on the coridor

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Acetate; Light condition: IR; Replicate: B; Time: 0h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 874751.4 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 58079.7 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Acetate; Light condition: IR; Replicate: C; Time: 0h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1097587.8 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 58079.7 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Control; Light condition: Dark; Replicate: A; Time: 24h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1277753.4 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 129197.7 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Control; Light condition: Dark; Replicate: B; Time: 24h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1479254.4 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 149347.8 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Control; Light condition: Dark; Replicate: C; Time: 24h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1536148.8 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 152903.7 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Control; Light condition: IR; Replicate: A; Time: 24h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1395098.1 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 110232.9 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Control; Light condition: IR; Replicate: B; Time: 24h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1305015.3 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 119715.3 cells per ml; epifluorescent microscopy

text: aerobic

text: intital denaturation:98degC_3min;denaturation:98degC_10s; annealing:52degC_30s;elongation:72degC_30s;number of cycles:30; final elongation:72degC_5min

text: pufM_uniF: GGNAAYYTNTWYTAYAAY; pufM_uniR: YCCATNGTCCANCKCCARAACCNTTYCA

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: DADA2 version 1.18.0; function: removeBimeraDenovo; method=pooled

text: Treatment: Control; Light condition: IR; Replicate: C; Time: 24h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 2105092.8 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 177795 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

text: Centre Algatech, freezere on the coridor

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Dilution; Light condition: Dark; Replicate: A; Time: 24h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1585931.4 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 120900.6 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Dilution; Light condition: Dark; Replicate: B; Time: 24h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1411692.3 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 107862.3 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Dilution; Light condition: Dark; Replicate: C; Time: 24h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1209006 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 79415.1 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Dilution; Light condition: IR; Replicate: A; Time: 24h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1657049.4 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 149347.8 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Dilution; Light condition: IR; Replicate: B; Time: 24h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1389171.6 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 136309.5 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Dilution; Light condition: IR; Replicate: C; Time: 24h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1386801 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 98379.9 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column