characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: SRA accession

text: Estuarine

text: Site 3 P zone rep a tech 4

text: soil

text: Germany

text: metagenome

characteristic: investigation type

text: estuarine marsh

characteristic: local environmental context

text: estuary metagenome

characteristic: project name

text: estuary metagenome

text: NovaSeq 6000 S4

characteristic: sequencing method

text: soil

characteristic: soil environmental package


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

tag: Namespace:Leibniz Institute of Freshwater Ecology and Inland Fisheries

characteristic: Submitter Id

text: Estuarine

characteristic: broad-scale environmental context

characteristic: collection date

unit: m

characteristic: depth

text: Site 3 p zone rep c tech 4

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Germany

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

text: estuarine marsh

text: estuary metagenome

characteristic: organism

text: Metagenomes from Elbe Estuary Particles (2021-2022)

text: estuary metagenome

text: NovaSeq 6000 S4

text: soil

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Guy's and St Thomas' Trust

characteristic: INSDC center name

text: public

characteristic: SRA accession

tag: Namespace:Guy's and St Thomas' Trust

text: Performance characteristics

text: United Kingdom

text: metagenome

text: Swabs

text: metagenome


characteristic: ENA-CHECKLIST

text: Guy's and St Thomas' Trust

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Submitter Id

text: Performance characteristics

text: United Kingdom

text: metagenome

text: Sputum

text: metagenome


text: Guy's and St Thomas' Trust

text: public

characteristic: collection date

text: Performance characteristics

characteristic: description

text: United Kingdom

text: metagenome

text: swabs

text: metagenome


text: Guy's and St Thomas' Trust

text: public

text: Performance characteristics

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: metagenome

text: swab

text: metagenome


text: Guy's and St Thomas' Trust

text: public

text: Performance characteristics

text: United Kingdom

text: metagenome

text: BAL

text: metagenome


text: Guy's and St Thomas' Trust

text: public

text: Performance characteristics

text: United Kingdom

text: metagenome

text: NT

text: metagenome


text: Guy's and St Thomas' Trust

text: public

text: Performance characteristics

text: United Kingdom

text: metagenome

text: NTS

text: metagenome


text: Guy's and St Thomas' Trust

text: public

text: Performance characteristics

text: United Kingdom

text: metagenome

text: PF

text: metagenome


text: Guy's and St Thomas' Trust

text: public

text: Performance characteristics

text: United Kingdom

text: metagenome

text: NDL

text: metagenome


text: Guy's and St Thomas' Trust

text: public

text: Performance characteristics

text: United Kingdom

text: metagenome

text: BAL/WS

text: metagenome


text: Guy's and St Thomas' Trust

text: public

text: Performance characteristics

text: United Kingdom

text: metagenome

text: ETT

text: metagenome


text: Guy's and St Thomas' Trust

text: public

text: Limit of detection

text: United Kingdom

text: metagenome

text: BAL

text: metagenome


text: Guy's and St Thomas' Trust

text: public

text: Negative control

text: United Kingdom

text: metagenome

text: NTC

text: metagenome


text: Guy's and St Thomas' Trust

text: public

text: Prospective study

text: United Kingdom

text: metagenome

text: BAL

text: metagenome


text: Guy's and St Thomas' Trust

text: public

text: Prospective study

text: United Kingdom

text: metagenome

text: PF

text: metagenome


text: Guy's and St Thomas' Trust

text: public

text: Prospective study

text: United Kingdom

text: metagenome

text: Sputum

text: metagenome


text: Guy's and St Thomas' Trust

text: public

text: Prospective study

text: United Kingdom

text: metagenome

text: ETT

text: metagenome


text: Guy's and St Thomas' Trust

text: public

text: Prospective study

text: United Kingdom

text: metagenome

text: NBL

text: metagenome


text: University of Vienna

text: public

tag: Namespace:University of Vienna

text: marine pelagic biome (ENVO:01000023)

characteristic: broad-scale environmental context

text: Inoculum seawater microbial community

text: Slovenia

text: marine metagenome

text: Bacterial degradation of ctenophore Mnemiopsis leidyi organic matter

characteristic: project name

text: marine metagenome

characteristic: sequencing method


Control

text: University of Vienna

text: public

text: Control

text: marine pelagic biome (ENVO:01000023)

text: Merged DNA from non-amended microcosms

text: Slovenia

text: metagenome

characteristic: organism

text: metagenome

text: Control

characteristic: title


Cteno-OM

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: University of Vienna

text: public

text: Cteno-OM

text: marine pelagic biome (ENVO:01000023)

text: Merged DNA from ammended microcosms

text: Slovenia

text: metagenome

text: metagenome

text: Cteno-OM


characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

text: public

tag: Namespace:UNIVERSITY OF SZEGED

text: ATCGCCTACCGTGAC / barcode / AGAGTTTGATCMTGGCTCAG; ATCGCCTACCGTGAC / barcode / CGGTTACCTTGTTACGACTT

characteristic: adapters

text: Csongrad county

text: EMU

characteristic: chimera check software

text: dog

characteristic: common name

text: Dog stool sample of Toti

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

unit: years

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

characteristic: host scientific name

text: male

characteristic: host sex

text: not applicable

characteristic: host subject id

text: cross-breeding of Hungarian Puli with French and German herding dogs originated from 17. century

characteristic: host subspecific genetic lineage

characteristic: host taxid

text: 16S Barcoding Kit (SQK-RAB20�C4)

characteristic: library construction method

text: ligation based

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

text: 16SF(ATCGCCTACCGTGAC / barcode / AGAGTTTGATCMTGGCTCAG); 16SR(ATCGCCTACCGTGAC / barcode / CGGTTACCTTGTTACGACTT)

characteristic: multiplex identifiers

text: LongAmp Taq 2X Master Mix

characteristic: nucleic acid amplification

text: PureLink Microbiome DNA Purification Kit, Invitrogen

characteristic: nucleic acid extraction

text: Canis lupus familiaris

text: not collected

characteristic: organism count

text: initial denaturation:95degC_1min; denaturation=95degC_20�Csec; annealing=55deg_30sec; extension=65deg_2min

characteristic: pcr conditions

text: 27F(AGAGTTTGATCCTGGCTCAG); 1492R(GGTTACCTTGTTACGACTT)

characteristic: pcr primers

text: Comparative metagenomic profiling of canine gut microbial community using different sample preparation methodes

unit: hours

characteristic: sample storage duration

text: Canis lupus familiaris

text: software

characteristic: sequence quality check

text: Oxford Nanopore, SQK-RAB20�C4

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Canfam_toti_minion_v1_9_20�C21120�C9_invitrogen_I1_bc09


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

characteristic: host scientific name

text: male

characteristic: host sex

text: not applicable

text: 16S Barcoding Kit (SQK-RAB20�C4)

text: ligation based

text: Szeged

text: LongAmp Taq 2X Master Mix

text: Canis lupus familiaris

characteristic: organism

text: not collected

characteristic: project name

text: Canis lupus familiaris

text: software

text: Oxford Nanopore, SQK-RAB20�C4

characteristic: sequencing method

text: 16S rRNA

text: Canfam_toti_minion_v1_9_20�C21120�C9_invitrogen_I2_bc10

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

text: no

text: Hungary

text: stool

text: Hungarian herding terrier: Pumi

text: omnivore

text: Harsanyi-Tekergo Bator

text: Canis lupus familiaris

text: male

text: not applicable

characteristic: host subject id

characteristic: host subspecific genetic lineage

characteristic: host taxid

text: 16S Barcoding Kit (SQK-RAB20�C4)

characteristic: library construction method

text: ligation based

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

characteristic: multiplex identifiers

text: LongAmp Taq 2X Master Mix

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: Canis lupus familiaris

text: not collected

characteristic: organism count

characteristic: pcr conditions

characteristic: pcr primers

characteristic: sample storage duration

text: Canis lupus familiaris

text: software

characteristic: sequence quality check

text: Oxford Nanopore, SQK-RAB20�C4

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Canfam_toti_minion_v1_9_20�C21120�C9_invitrogen_I3_bc11


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

characteristic: host scientific name

text: male

characteristic: host sex

text: not applicable

text: 16S Barcoding Kit (SQK-RAB20�C4)

text: ligation based

text: Szeged

text: LongAmp Taq 2X Master Mix

text: Canis lupus familiaris

characteristic: organism

text: not collected

characteristic: project name

text: Canis lupus familiaris

text: software

text: Oxford Nanopore, SQK-RAB20�C4

characteristic: sequencing method

text: 16S rRNA

text: Canfam_toti_minion_v1_9_20�C21120�C9_invitrogen_I4_bc12

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

text: no

text: Hungary

text: stool

text: Hungarian herding terrier: Pumi

text: omnivore

text: Harsanyi-Tekergo Bator

text: Canis lupus familiaris

text: male

text: not applicable

characteristic: host subject id

characteristic: host subspecific genetic lineage

characteristic: host taxid

text: 16S Barcoding Kit (SQK-RAB20�C4)

characteristic: library construction method

text: ligation based

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

characteristic: multiplex identifiers

text: LongAmp Taq 2X Master Mix

characteristic: nucleic acid amplification

text: Macherey Nagel NucleoSpin DNA Stool kit

characteristic: nucleic acid extraction

text: Canis lupus familiaris

text: not collected

characteristic: organism count

characteristic: pcr conditions

characteristic: pcr primers

characteristic: sample storage duration

text: Canis lupus familiaris

text: software

characteristic: sequence quality check

text: Oxford Nanopore, SQK-RAB20�C4

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Canfam_toti_minion_v1_9_20�C21120�C9_macherey_nagel_MN1_bc05


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

characteristic: host scientific name

text: male

characteristic: host sex

text: not applicable

text: 16S Barcoding Kit (SQK-RAB20�C4)

text: ligation based

text: Szeged

text: LongAmp Taq 2X Master Mix

text: Macherey Nagel NucleoSpin DNA Stool kit

text: Canis lupus familiaris

characteristic: organism

text: not collected

characteristic: project name

text: Canis lupus familiaris

text: software

text: Oxford Nanopore, SQK-RAB20�C4

characteristic: sequencing method

text: 16S rRNA

text: Canfam_toti_minion_v1_9_20�C21120�C9_macherey_nagel_MN2_bc06

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

text: no

text: Hungary

text: stool

text: Hungarian herding terrier: Pumi

text: omnivore

text: Harsanyi-Tekergo Bator

text: Canis lupus familiaris

text: male

text: not applicable

characteristic: host subject id

characteristic: host subspecific genetic lineage

characteristic: host taxid

text: 16S Barcoding Kit (SQK-RAB20�C4)

characteristic: library construction method

text: ligation based

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

characteristic: multiplex identifiers

text: LongAmp Taq 2X Master Mix

characteristic: nucleic acid amplification

text: Macherey Nagel NucleoSpin DNA Stool kit

characteristic: nucleic acid extraction

text: Canis lupus familiaris

text: not collected

characteristic: organism count

characteristic: pcr conditions

characteristic: pcr primers

characteristic: sample storage duration

text: Canis lupus familiaris

text: software

characteristic: sequence quality check

text: Oxford Nanopore, SQK-RAB20�C4

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Canfam_toti_minion_v1_9_20�C21120�C9_macherey_nagel_MN3_bc07


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

characteristic: host scientific name

text: male

characteristic: host sex

text: not applicable

text: 16S Barcoding Kit (SQK-RAB20�C4)

text: ligation based

text: Szeged

text: LongAmp Taq 2X Master Mix

text: Macherey Nagel NucleoSpin DNA Stool kit

text: Canis lupus familiaris

characteristic: organism

text: not collected

characteristic: project name

text: Canis lupus familiaris

text: software

text: Oxford Nanopore, SQK-RAB20�C4

characteristic: sequencing method

text: 16S rRNA

text: Canfam_toti_minion_v1_9_20�C21120�C9_macherey_nagel_MN4_bc08

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

text: no

text: Hungary

text: stool

text: Hungarian herding terrier: Pumi

text: omnivore

text: Harsanyi-Tekergo Bator

text: Canis lupus familiaris

text: male

text: not applicable

characteristic: host subject id

characteristic: host subspecific genetic lineage

characteristic: host taxid

text: 16S Barcoding Kit (SQK-RAB20�C4)

characteristic: library construction method

text: ligation based

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

characteristic: multiplex identifiers

text: LongAmp Taq 2X Master Mix

characteristic: nucleic acid amplification

text: QIAamp Fast DNA Stool Mini Kit, Qiagen

characteristic: nucleic acid extraction

text: Canis lupus familiaris

text: not collected

characteristic: organism count

characteristic: pcr conditions

characteristic: pcr primers

characteristic: sample storage duration

text: Canis lupus familiaris

text: software

characteristic: sequence quality check

text: Oxford Nanopore, SQK-RAB20�C4

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Canfam_toti_minion_v1_9_20�C21120�C9_qiagen_Q1_bc01


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

characteristic: host scientific name

text: male

characteristic: host sex

text: not applicable

text: 16S Barcoding Kit (SQK-RAB20�C4)

text: ligation based

text: Szeged

text: LongAmp Taq 2X Master Mix

text: QIAamp Fast DNA Stool Mini Kit, Qiagen

text: Canis lupus familiaris

characteristic: organism

text: not collected

characteristic: project name

text: Canis lupus familiaris

text: software

text: Oxford Nanopore, SQK-RAB20�C4

characteristic: sequencing method

text: 16S rRNA

text: Canfam_toti_minion_v1_9_20�C21120�C9_qiagen_Q2_bc02

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ATCGCCTACCGTGAC / barcode / AGAGTTTGATCMTGGCTCAG; ATCGCCTACCGTGAC / barcode / CGGTTACCTTGTTACGACTT

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

text: no

text: Hungary

text: stool

text: Hungarian herding terrier: Pumi

text: omnivore

text: Harsanyi-Tekergo Bator

text: Canis lupus familiaris

text: male

text: not applicable

characteristic: host subject id

characteristic: host subspecific genetic lineage

characteristic: host taxid

text: 16S Barcoding Kit (SQK-RAB20�C4)

characteristic: library construction method

text: ligation based

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

characteristic: multiplex identifiers

text: LongAmp Taq 2X Master Mix

characteristic: nucleic acid amplification

text: QIAamp Fast DNA Stool Mini Kit, Qiagen

characteristic: nucleic acid extraction

text: Canis lupus familiaris

text: not collected

characteristic: organism count

characteristic: pcr conditions

characteristic: pcr primers

characteristic: sample storage duration

text: Canis lupus familiaris

text: software

characteristic: sequence quality check

text: Oxford Nanopore, SQK-RAB20�C4

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Canfam_toti_minion_v1_9_20�C21120�C9_qiagen_Q3_bc03


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

characteristic: host scientific name

text: male

characteristic: host sex

text: not applicable

text: cross-breeding of Hungarian Puli with French and German herding dogs originated from 17. century

text: 16S Barcoding Kit (SQK-RAB20�C4)

text: ligation based

text: Szeged

text: 16SF(ATCGCCTACCGTGAC / barcode / AGAGTTTGATCMTGGCTCAG); 16SR(ATCGCCTACCGTGAC / barcode / CGGTTACCTTGTTACGACTT)

text: LongAmp Taq 2X Master Mix

text: QIAamp Fast DNA Stool Mini Kit, Qiagen

text: Canis lupus familiaris

characteristic: organism

text: not collected

characteristic: project name

text: Canis lupus familiaris

text: software

text: Oxford Nanopore, SQK-RAB20�C4

characteristic: sequencing method

text: 16S rRNA

text: Canfam_toti_minion_v1_9_20�C21120�C9_qiagen_Q4_bc04

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

text: no

text: Hungary

text: stool

text: Hungarian herding terrier: Pumi

text: omnivore

text: Harsanyi-Tekergo Bator

text: Canis lupus familiaris

text: male

text: not applicable

characteristic: host subject id

characteristic: host subspecific genetic lineage

characteristic: host taxid

text: 16S Barcoding Kit (SQK-RAB20�C4)

characteristic: library construction method

text: ligation based

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

characteristic: multiplex identifiers

text: LongAmp Taq 2X Master Mix

characteristic: nucleic acid amplification

text: Quick-DNA HMW MagBead Kit, Zymo

characteristic: nucleic acid extraction

text: Canis lupus familiaris

text: not collected

characteristic: organism count

text: initial denaturation:95degC_1min; denaturation=95degC_20�Csec; annealing=55deg_30sec; extension=65deg_2min

characteristic: pcr conditions

text: 27F(AGAGTTTGATCCTGGCTCAG); 1492R(GGTTACCTTGTTACGACTT)

characteristic: pcr primers

characteristic: sample storage duration

text: Canis lupus familiaris

text: software

characteristic: sequence quality check

text: Oxford Nanopore, SQK-RAB20�C4

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Canfam_toti_minion_v1_9_20�C211215_zymo_Z1_bc01


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

characteristic: host scientific name

text: male

characteristic: host sex

text: not applicable

text: 16S Barcoding Kit (SQK-RAB20�C4)

text: ligation based

text: Szeged

text: LongAmp Taq 2X Master Mix

text: Quick-DNA HMW MagBead Kit, Zymo

text: Canis lupus familiaris

characteristic: organism

text: not collected

text: Comparative metagenomic profiling of canine gut microbial community using different sample preparation methodes

characteristic: project name

text: Canis lupus familiaris

text: software

text: Oxford Nanopore, SQK-RAB20�C4

characteristic: sequencing method

text: 16S rRNA

text: Canfam_toti_minion_v1_9_20�C211215_zymo_Z2_bc02

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

text: no

text: Hungary

text: stool

text: Hungarian herding terrier: Pumi

text: omnivore

text: Harsanyi-Tekergo Bator

text: Canis lupus familiaris

text: male

text: not applicable

characteristic: host subject id

characteristic: host subspecific genetic lineage

characteristic: host taxid

text: 16S Barcoding Kit (SQK-RAB20�C4)

characteristic: library construction method

text: ligation based

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

characteristic: multiplex identifiers

text: LongAmp Taq 2X Master Mix

characteristic: nucleic acid amplification

text: Quick-DNA HMW MagBead Kit, Zymo

characteristic: nucleic acid extraction

text: Canis lupus familiaris

text: not collected

characteristic: organism count

characteristic: pcr conditions

characteristic: pcr primers

characteristic: sample storage duration

text: Canis lupus familiaris

text: software

characteristic: sequence quality check

text: Oxford Nanopore, SQK-RAB20�C4

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Canfam_toti_minion_v1_9_20�C211215_zymo_Z3_bc03


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

characteristic: host scientific name

text: male

characteristic: host sex

text: not applicable

text: 16S Barcoding Kit (SQK-RAB20�C4)

text: ligation based

text: Szeged

text: LongAmp Taq 2X Master Mix

text: Quick-DNA HMW MagBead Kit, Zymo

text: Canis lupus familiaris

characteristic: organism

text: not collected

characteristic: project name

text: Canis lupus familiaris

text: software

text: Oxford Nanopore, SQK-RAB20�C4

characteristic: sequencing method

text: 16S rRNA

text: Canfam_toti_minion_v1_9_20�C211215_zymo_Z4_bc04

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

text: no

text: Hungary

text: stool

text: Hungarian herding terrier: Pumi

text: omnivore

text: Harsanyi-Tekergo Bator

text: Canis lupus familiaris

text: male

text: not applicable

characteristic: host subject id

characteristic: host subspecific genetic lineage

characteristic: host taxid

text: 16S Barcoding Kit (SQK-RAB20�C4)

characteristic: library construction method

text: ligation based

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

characteristic: multiplex identifiers

text: LongAmp Taq 2X Master Mix

characteristic: nucleic acid amplification

text: PureLink Microbiome DNA Purification Kit, Invitrogen

characteristic: nucleic acid extraction

text: Canis lupus familiaris

text: not collected

characteristic: organism count

characteristic: pcr conditions

characteristic: pcr primers

characteristic: sample storage duration

text: Canis lupus familiaris

text: software

characteristic: sequence quality check

text: Oxford Nanopore, SQK-RAB20�C4

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Canfam_toti_minion_v1_9_20�C220�C325_invitrogen_I1_bc09


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

characteristic: host scientific name

text: male

characteristic: host sex

text: not applicable

text: 16S Barcoding Kit (SQK-RAB20�C4)

text: ligation based

text: Szeged

text: LongAmp Taq 2X Master Mix

text: Canis lupus familiaris

characteristic: organism

text: not collected

characteristic: project name

text: Canis lupus familiaris

text: software

text: Oxford Nanopore, SQK-RAB20�C4

characteristic: sequencing method

text: 16S rRNA

text: Canfam_toti_minion_v1_9_20�C220�C325_invitrogen_I2_bc02

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

text: no

text: Hungary

text: stool

text: Hungarian herding terrier: Pumi

text: omnivore

text: Harsanyi-Tekergo Bator

text: Canis lupus familiaris

text: male

text: not applicable

characteristic: host subject id

characteristic: host subspecific genetic lineage

characteristic: host taxid

text: 16S Barcoding Kit (SQK-RAB20�C4)

characteristic: library construction method

text: ligation based

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

characteristic: multiplex identifiers

text: LongAmp Taq 2X Master Mix

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: Canis lupus familiaris

text: not collected

characteristic: organism count

characteristic: pcr conditions

characteristic: pcr primers

characteristic: sample storage duration

text: Canis lupus familiaris

text: software

characteristic: sequence quality check

text: Oxford Nanopore, SQK-RAB20�C4

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Canfam_toti_minion_v1_9_20�C220�C325_invitrogen_I3_bc03


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

characteristic: host scientific name

text: male

characteristic: host sex

text: not applicable

text: 16S Barcoding Kit (SQK-RAB20�C4)

text: ligation based

text: Szeged

text: LongAmp Taq 2X Master Mix

text: Canis lupus familiaris

characteristic: organism

text: not collected

characteristic: project name

text: Canis lupus familiaris

text: software

text: Oxford Nanopore, SQK-RAB20�C4

characteristic: sequencing method

text: 16S rRNA

text: Canfam_toti_minion_v1_9_20�C220�C325_invitrogen_I4_bc04

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ATCGCCTACCGTGAC / barcode / AGAGTTTGATCMTGGCTCAG; ATCGCCTACCGTGAC / barcode / CGGTTACCTTGTTACGACTT

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

text: no

text: Hungary

text: stool

text: Hungarian herding terrier: Pumi

text: omnivore

text: Harsanyi-Tekergo Bator

text: Canis lupus familiaris

text: male

text: not applicable

characteristic: host subject id

characteristic: host subspecific genetic lineage

characteristic: host taxid

text: 16S Barcoding Kit (SQK-RAB20�C4)

characteristic: library construction method

text: ligation based

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

characteristic: multiplex identifiers

text: LongAmp Taq 2X Master Mix

characteristic: nucleic acid amplification

text: Macherey Nagel NucleoSpin DNA Stool kit

characteristic: nucleic acid extraction

text: Canis lupus familiaris

text: not collected

characteristic: organism count

characteristic: pcr conditions

characteristic: pcr primers

characteristic: sample storage duration

text: Canis lupus familiaris

text: software

characteristic: sequence quality check

text: Oxford Nanopore, SQK-RAB20�C4

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Canfam_toti_minion_v1_9_20�C220�C325_macherey_nagel_MN1_bc05


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

characteristic: host scientific name

text: male

characteristic: host sex

text: not applicable

text: cross-breeding of Hungarian Puli with French and German herding dogs originated from 17. century

text: 16S Barcoding Kit (SQK-RAB20�C4)

text: ligation based

text: Szeged

text: LongAmp Taq 2X Master Mix

text: Macherey Nagel NucleoSpin DNA Stool kit

text: Canis lupus familiaris

characteristic: organism

text: not collected

characteristic: project name

text: Canis lupus familiaris

text: software

text: Oxford Nanopore, SQK-RAB20�C4

characteristic: sequencing method

text: 16S rRNA

text: Canfam_toti_minion_v1_9_20�C220�C325_macherey_nagel_MN2_bc10

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

text: no

text: Hungary

text: stool

text: Hungarian herding terrier: Pumi

text: omnivore

text: Harsanyi-Tekergo Bator

text: Canis lupus familiaris

text: male

text: not applicable

characteristic: host subject id

characteristic: host subspecific genetic lineage

characteristic: host taxid

text: 16S Barcoding Kit (SQK-RAB20�C4)

characteristic: library construction method

text: ligation based

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

text: 16SF(ATCGCCTACCGTGAC / barcode / AGAGTTTGATCMTGGCTCAG); 16SR(ATCGCCTACCGTGAC / barcode / CGGTTACCTTGTTACGACTT)

characteristic: multiplex identifiers

text: LongAmp Taq 2X Master Mix

characteristic: nucleic acid amplification

text: Macherey Nagel NucleoSpin DNA Stool kit

characteristic: nucleic acid extraction

text: Canis lupus familiaris

text: not collected

characteristic: organism count

characteristic: pcr conditions

characteristic: pcr primers

characteristic: sample storage duration

text: Canis lupus familiaris

text: software

characteristic: sequence quality check

text: Oxford Nanopore, SQK-RAB20�C4

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Canfam_toti_minion_v1_9_20�C220�C325_macherey_nagel_MN3_bc11


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

characteristic: host scientific name

text: male

characteristic: host sex

text: not applicable

text: 16S Barcoding Kit (SQK-RAB20�C4)

text: ligation based

text: Szeged

text: LongAmp Taq 2X Master Mix

text: Macherey Nagel NucleoSpin DNA Stool kit

text: Canis lupus familiaris

characteristic: organism

text: not collected

text: initial denaturation:95degC_1min; denaturation=95degC_20�Csec; annealing=55deg_30sec; extension=65deg_2min

text: 27F(AGAGTTTGATCCTGGCTCAG); 1492R(GGTTACCTTGTTACGACTT)

characteristic: project name

text: Canis lupus familiaris

text: software

text: Oxford Nanopore, SQK-RAB20�C4

characteristic: sequencing method

text: 16S rRNA

text: Canfam_toti_minion_v1_9_20�C220�C325_macherey_nagel_MN4_bc12

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

text: no

text: Hungary

text: stool

text: Hungarian herding terrier: Pumi

text: omnivore

text: Harsanyi-Tekergo Bator

text: Canis lupus familiaris

text: male

text: not applicable

characteristic: host subject id

characteristic: host subspecific genetic lineage

characteristic: host taxid

text: 16S Barcoding Kit (SQK-RAB20�C4)

characteristic: library construction method

text: ligation based

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

characteristic: multiplex identifiers

text: LongAmp Taq 2X Master Mix

characteristic: nucleic acid amplification

text: Quick-DNA HMW MagBead Kit, Zymo

characteristic: nucleic acid extraction

text: Canis lupus familiaris

text: not collected

characteristic: organism count

characteristic: pcr conditions

characteristic: pcr primers

text: Comparative metagenomic profiling of canine gut microbial community using different sample preparation methodes

characteristic: sample storage duration

text: Canis lupus familiaris

text: software

characteristic: sequence quality check

text: Oxford Nanopore, SQK-RAB20�C4

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Canfam_toti_minion_v1_9_20�C220�C325_zymo_Z1_bc01


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

characteristic: host scientific name

text: male

characteristic: host sex

text: not applicable

text: 16S Barcoding Kit (SQK-RAB20�C4)

text: ligation based

text: Szeged

text: LongAmp Taq 2X Master Mix

text: Quick-DNA HMW MagBead Kit, Zymo

text: Canis lupus familiaris

characteristic: organism

text: not collected

characteristic: project name

text: Canis lupus familiaris

text: software

text: Oxford Nanopore, SQK-RAB20�C4

characteristic: sequencing method

text: 16S rRNA

text: Canfam_toti_minion_v1_9_20�C220�C325_zymo_Z2_bc06

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

text: no

text: Hungary

text: stool

text: Hungarian herding terrier: Pumi

text: omnivore

text: Harsanyi-Tekergo Bator

text: Canis lupus familiaris

text: male

text: not applicable

characteristic: host subject id

characteristic: host subspecific genetic lineage

characteristic: host taxid

text: 16S Barcoding Kit (SQK-RAB20�C4)

characteristic: library construction method

text: ligation based

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

characteristic: multiplex identifiers

text: LongAmp Taq 2X Master Mix

characteristic: nucleic acid amplification

text: Quick-DNA HMW MagBead Kit, Zymo

characteristic: nucleic acid extraction

text: Canis lupus familiaris

text: not collected

characteristic: organism count

characteristic: pcr conditions

characteristic: pcr primers

characteristic: sample storage duration

text: Canis lupus familiaris

text: software

characteristic: sequence quality check

text: Oxford Nanopore, SQK-RAB20�C4

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Canfam_toti_minion_v1_9_20�C220�C325_zymo_Z3_bc07


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

characteristic: host scientific name

text: male

characteristic: host sex

text: not applicable

text: 16S Barcoding Kit (SQK-RAB20�C4)

text: ligation based

text: Szeged

text: LongAmp Taq 2X Master Mix

text: Quick-DNA HMW MagBead Kit, Zymo

text: Canis lupus familiaris

characteristic: organism

text: not collected

characteristic: project name

text: Canis lupus familiaris

text: software

text: Oxford Nanopore, SQK-RAB20�C4

characteristic: sequencing method

text: 16S rRNA

text: Canfam_toti_minion_v1_9_20�C220�C325_zymo_Z4_bc08

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: TCTGTATCTCTATGTG; ATGTGCGTGTGTGTCT

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

text: no

text: Hungary

text: stool

text: Hungarian herding terrier: Pumi

text: omnivore

text: Harsanyi-Tekergo Bator

text: Canis lupus familiaris

text: male

text: not applicable

characteristic: host subject id

characteristic: host subspecific genetic lineage

characteristic: host taxid

text: software

characteristic: library construction method

text: ligation based

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

text: 16S_Fw_1007(TCTGTATCTCTATGTG); 16S_Rev_1056(ATGTGCGTGTGTGTCT)

characteristic: multiplex identifiers

text: LongAmp Taq 2X Master Mix

characteristic: nucleic acid amplification

text: PureLink Microbiome DNA Purification Kit, Invitrogen

characteristic: nucleic acid extraction

text: Canis lupus familiaris

text: not collected

characteristic: organism count

characteristic: pcr conditions

text: 27F(AGRGTTTGATYMTGGCTCAG); 1492R(GGYTACCTTGTTACGACTT)

characteristic: pcr primers

characteristic: sample storage duration

text: Canis lupus familiaris

text: software

characteristic: sequence quality check

text: PacBio RS II SMRT DNA Sequencing System, P6-C4

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Canfam_toti_pacbio_v1_v9_20�C220�C722_invitrogen_I1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ACAGTCGAGCGCTGCG; ATGTGCGTGTGTGTCT

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

characteristic: host scientific name

text: male

characteristic: host sex

text: not applicable

text: cross-breeding of Hungarian Puli with French and German herding dogs originated from 17. century

text: software

text: ligation based

text: Szeged

text: 16S_Fw_1008(ACAGTCGAGCGCTGCG); 16S_Rev_1056(ATGTGCGTGTGTGTCT)

text: LongAmp Taq 2X Master Mix

text: Canis lupus familiaris

characteristic: organism

text: not collected

characteristic: project name

text: Canis lupus familiaris

text: software

characteristic: sequencing method

text: 16S rRNA

text: Canfam_toti_pacbio_v1_v9_20�C220�C722_invitrogen_I2

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ACACTAGATCGCGTGT; ATGTGCGTGTGTGTCT

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

text: no

text: Hungary

text: stool

text: Hungarian herding terrier: Pumi

text: omnivore

text: Harsanyi-Tekergo Bator

text: Canis lupus familiaris

text: male

text: not applicable

characteristic: host subject id

characteristic: host subspecific genetic lineage

characteristic: host taxid

text: software

characteristic: library construction method

text: ligation based

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

text: 16S_Fw_1012(ACACTAGATCGCGTGT); 16S_Rev_1056(ATGTGCGTGTGTGTCT)

characteristic: multiplex identifiers

text: LongAmp Taq 2X Master Mix

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: Canis lupus familiaris

text: not collected

characteristic: organism count

characteristic: pcr conditions

characteristic: pcr primers

characteristic: sample storage duration

text: Canis lupus familiaris

text: software

characteristic: sequence quality check

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Canfam_toti_pacbio_v1_v9_20�C220�C722_invitrogen_I3


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: CGCATGACACGTGTGT; ATGTGCGTGTGTGTCT

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

characteristic: host scientific name

text: male

characteristic: host sex

text: not applicable

text: software

text: ligation based

text: Szeged

text: 16S_Fw_1015(CGCATGACACGTGTGT); 16S_Rev_1056(ATGTGCGTGTGTGTCT)

text: LongAmp Taq 2X Master Mix

text: Canis lupus familiaris

characteristic: organism

text: not collected

text: initial denaturation:95degC_1min; denaturation=95degC_20�Csec; annealing=55deg_30sec; extension=65deg_2min

characteristic: project name

text: Canis lupus familiaris

text: software

characteristic: sequencing method

text: 16S rRNA

text: Canfam_toti_pacbio_v1_v9_20�C220�C722_invitrogen_I4

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: CACGACACGACGATGT; ATGTGCGTGTGTGTCT

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

text: no

text: Hungary

text: stool

text: Hungarian herding terrier: Pumi

text: omnivore

text: Harsanyi-Tekergo Bator

text: Canis lupus familiaris

text: male

text: not applicable

characteristic: host subject id

characteristic: host subspecific genetic lineage

characteristic: host taxid

text: software

characteristic: library construction method

text: ligation based

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

text: 16S_Fw_1020(CACGACACGACGATGT); 16S_Rev_1056(ATGTGCGTGTGTGTCT)

characteristic: multiplex identifiers

text: LongAmp Taq 2X Master Mix

characteristic: nucleic acid amplification

text: Macherey Nagel NucleoSpin DNA Stool kit

characteristic: nucleic acid extraction

text: Canis lupus familiaris

text: not collected

characteristic: organism count

characteristic: pcr conditions

characteristic: pcr primers

text: Comparative metagenomic profiling of canine gut microbial community using different sample preparation methodes

characteristic: sample storage duration

text: Canis lupus familiaris

text: software

characteristic: sequence quality check

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Canfam_toti_pacbio_v1_v9_20�C220�C722_macherey_nagel_MN1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: CACTCACGTGTGATAT; ATGTGCGTGTGTGTCT

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

characteristic: host scientific name

text: male

characteristic: host sex

text: not applicable

text: software

text: ligation based

text: Szeged

text: 16S_Fw_1022(CACTCACGTGTGATAT); 16S_Rev_1056(ATGTGCGTGTGTGTCT)

text: LongAmp Taq 2X Master Mix

text: Macherey Nagel NucleoSpin DNA Stool kit

text: Canis lupus familiaris

characteristic: organism

text: not collected

characteristic: project name

text: Canis lupus familiaris

text: software

characteristic: sequencing method

text: 16S rRNA

text: Canfam_toti_pacbio_v1_v9_20�C220�C722_macherey_nagel_MN2

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: CATGTAGAGCAGAGAG; ATGTGCGTGTGTGTCT

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

text: no

text: Hungary

text: stool

text: Hungarian herding terrier: Pumi

text: omnivore

text: Harsanyi-Tekergo Bator

text: Canis lupus familiaris

text: male

text: not applicable

characteristic: host subject id

characteristic: host subspecific genetic lineage

characteristic: host taxid

text: software

characteristic: library construction method

text: ligation based

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

text: 16S_Fw_1024(CATGTAGAGCAGAGAG); 16S_Rev_1056(ATGTGCGTGTGTGTCT)

characteristic: multiplex identifiers

text: LongAmp Taq 2X Master Mix

characteristic: nucleic acid amplification

text: Macherey Nagel NucleoSpin DNA Stool kit

characteristic: nucleic acid extraction

text: Canis lupus familiaris

text: not collected

characteristic: organism count

characteristic: pcr conditions

characteristic: pcr primers

characteristic: sample storage duration

text: Canis lupus familiaris

text: software

characteristic: sequence quality check

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Canfam_toti_pacbio_v1_v9_20�C220�C722_macherey_nagel_MN3


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: CACTCGACTCTCGCGT; CTCTCAGACGCTCGTC

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

characteristic: host scientific name

text: male

characteristic: host sex

text: not applicable

text: software

text: ligation based

text: Szeged

text: 16S_Fw_1005(CACTCGACTCTCGCGT); 16S_Rev_1057(CTCTCAGACGCTCGTC)

text: LongAmp Taq 2X Master Mix

text: Macherey Nagel NucleoSpin DNA Stool kit

text: Canis lupus familiaris

characteristic: organism

text: not collected

text: 27F(AGRGTTTGATYMTGGCTCAG); 1492R(GGYTACCTTGTTACGACTT)

characteristic: project name

text: Canis lupus familiaris

text: software

text: PacBio RS II SMRT DNA Sequencing System, P6-C4

characteristic: sequencing method

text: 16S rRNA

text: Canfam_toti_pacbio_v1_v9_20�C220�C722_macherey_nagel_MN4

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: TCTGTATCTCTATGTG; CTCTCAGACGCTCGTC

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

text: no

text: Hungary

text: stool

text: Hungarian herding terrier: Pumi

text: omnivore

text: Harsanyi-Tekergo Bator

text: Canis lupus familiaris

text: male

text: not applicable

characteristic: host subject id

text: cross-breeding of Hungarian Puli with French and German herding dogs originated from 17. century

characteristic: host subspecific genetic lineage

characteristic: host taxid

text: software

characteristic: library construction method

text: ligation based

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

text: 16S_Fw_1007(TCTGTATCTCTATGTG); 16S_Rev_1057(CTCTCAGACGCTCGTC)

characteristic: multiplex identifiers

text: LongAmp Taq 2X Master Mix

characteristic: nucleic acid amplification

text: Quick-DNA HMW MagBead Kit, Zymo

characteristic: nucleic acid extraction

text: Canis lupus familiaris

text: not collected

characteristic: organism count

characteristic: pcr conditions

characteristic: pcr primers

characteristic: sample storage duration

text: Canis lupus familiaris

text: software

characteristic: sequence quality check

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Canfam_toti_pacbio_v1_v9_20�C220�C722_zymo_Z1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ACAGTCGAGCGCTGCG; CTCTCAGACGCTCGTC

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

characteristic: host scientific name

text: male

characteristic: host sex

text: not applicable

text: software

text: ligation based

text: Szeged

text: 16S_Fw_1008(ACAGTCGAGCGCTGCG); 16S_Rev_1057(CTCTCAGACGCTCGTC)

text: LongAmp Taq 2X Master Mix

text: Quick-DNA HMW MagBead Kit, Zymo

text: Canis lupus familiaris

characteristic: organism

text: not collected

characteristic: project name

text: Canis lupus familiaris

text: software

characteristic: sequencing method

text: 16S rRNA

text: Canfam_toti_pacbio_v1_v9_20�C220�C722_zymo_Z2

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ACACTAGATCGCGTGT; CTCTCAGACGCTCGTC

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

text: no

text: Hungary

text: stool

text: Hungarian herding terrier: Pumi

text: omnivore

text: Harsanyi-Tekergo Bator

text: Canis lupus familiaris

text: male

text: not applicable

characteristic: host subject id

characteristic: host subspecific genetic lineage

characteristic: host taxid

text: software

characteristic: library construction method

text: ligation based

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

text: 16S_Fw_1012(ACACTAGATCGCGTGT); 16S_Rev_1057(CTCTCAGACGCTCGTC)

characteristic: multiplex identifiers

text: LongAmp Taq 2X Master Mix

characteristic: nucleic acid amplification

text: Quick-DNA HMW MagBead Kit, Zymo

characteristic: nucleic acid extraction

text: Canis lupus familiaris

text: not collected

characteristic: organism count

text: initial denaturation:95degC_1min; denaturation=95degC_20�Csec; annealing=55deg_30sec; extension=65deg_2min

characteristic: pcr conditions

characteristic: pcr primers

characteristic: sample storage duration

text: Canis lupus familiaris

text: software

characteristic: sequence quality check

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Canfam_toti_pacbio_v1_v9_20�C220�C722_zymo_Z3


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: CGCATGACACGTGTGT; CTCTCAGACGCTCGTC

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

text: EMU

characteristic: chimera check software

characteristic: collection date

text: dog

characteristic: common name

text: Dog stool sample of Toti

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

characteristic: host scientific name

text: male

characteristic: host sex

text: not applicable

text: software

text: ligation based

text: Szeged

text: 16S_Fw_1015(CGCATGACACGTGTGT); 16S_Rev_1057(CTCTCAGACGCTCGTC)

text: LongAmp Taq 2X Master Mix

text: Quick-DNA HMW MagBead Kit, Zymo

text: Canis lupus familiaris

characteristic: organism

text: not collected

text: Comparative metagenomic profiling of canine gut microbial community using different sample preparation methodes

characteristic: project name

text: Canis lupus familiaris

text: software

characteristic: sequencing method

text: 16S rRNA

text: Canfam_toti_pacbio_v1_v9_20�C220�C722_zymo_Z4

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 16SFw 5'-(ATCGCCTACCGTGAC - barcode - AGAGTTTGATCMTGGCTCAG); 16S Rev 5'-(ATCGCCTACCGTGAC - barcode - CGGTTACCTTGTTACGACTT)

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

characteristic: collection date

text: ZymoBIOMICS Microbial Community Standard isolated by Invitrogen PureLink Microbiome DNA Purification Kit

text: ZymoBIOMICS Microbial Community DNA Standard

text: Hungary

text: 16S Barcoding Kit (SQK-RAB204)

characteristic: library construction method

text: ligation based

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

text: GTGTTACCGTGGGAATGAATCCTT

characteristic: multiplex identifiers

text: LongAmp Taq 2X Master Mix

characteristic: nucleic acid amplification

text: PureLink Microbiome DNA Purification Kit, Invitrogen

characteristic: nucleic acid extraction

text: mixed culture metagenome

characteristic: pcr conditions

text: 27F 5'-(AGAGTTTGATCCTGGCTCAG); 1492R 5'-(GGTTACCTTGTTACGACTT)

characteristic: pcr primers

text: mixed culture metagenome

text: software

characteristic: sequence quality check

text: Oxford Nanopore, SQK-RAB20�C4

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: BACMIX_minion_v1_9_20220720_invitrogen_I1_bc07


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Csongrad county

characteristic: description

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: 16S Barcoding Kit (SQK-RAB204)

text: ligation based

text: Szeged

text: TTCAGGGAACAAACCAAGTTACGT

text: LongAmp Taq 2X Master Mix

text: mixed culture metagenome

characteristic: organism

characteristic: project name

text: mixed culture metagenome

text: software

text: Oxford Nanopore, SQK-RAB20�C4

characteristic: sequencing method

text: 16S rRNA

text: BACMIX_minion_v1_9_20220720_invitrogen_I2_bc08

characteristic: title


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF SZEGED

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

characteristic: collection date

text: Hungary

text: 16S Barcoding Kit (SQK-RAB204)

characteristic: library construction method

text: ligation based

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

text: AACTAGGCACAGCGAGTCTTGGTT

characteristic: multiplex identifiers

text: LongAmp Taq 2X Master Mix

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: mixed culture metagenome

characteristic: pcr conditions

characteristic: pcr primers

text: mixed culture metagenome

text: software

characteristic: sequence quality check

text: Oxford Nanopore, SQK-RAB20�C4

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: BACMIX_minion_v1_9_20220720_invitrogen_I3_bc09


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Csongrad county

text: ZymoBIOMICS Microbial Community Standard isolated by Macherey-Nagel NucleoSpin DNA Stool Mini kit

characteristic: description

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: 16S Barcoding Kit (SQK-RAB204)

text: ligation based

text: Szeged

text: TTCGGATTCTATCGTGTTTCCCTA

text: LongAmp Taq 2X Master Mix

text: Macherey Nagel NucleoSpin DNA Stool kit

text: mixed culture metagenome

characteristic: organism

characteristic: project name

text: mixed culture metagenome

text: software

text: Oxford Nanopore, SQK-RAB20�C4

characteristic: sequencing method

text: 16S rRNA

text: BACMIX_minion_v1_9_20220720_macherey_nagel_MN1_bc04

characteristic: title


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF SZEGED

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

characteristic: collection date

text: Hungary

text: 16S Barcoding Kit (SQK-RAB204)

characteristic: library construction method

text: ligation based

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

text: CTTGTCCAGGGTTTGTGTAACCTT

characteristic: multiplex identifiers

text: LongAmp Taq 2X Master Mix

characteristic: nucleic acid amplification

text: Macherey Nagel NucleoSpin DNA Stool kit

characteristic: nucleic acid extraction

text: mixed culture metagenome

characteristic: pcr conditions

characteristic: pcr primers

text: mixed culture metagenome

text: software

characteristic: sequence quality check

text: Oxford Nanopore, SQK-RAB20�C4

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: BACMIX_minion_v1_9_20220720_macherey_nagel_MN2_bc05


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Csongrad county

characteristic: description

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: 16S Barcoding Kit (SQK-RAB204)

text: ligation based

text: Szeged

text: TTCTCGCAAAGGCAGAAAGTAGTC

text: LongAmp Taq 2X Master Mix

text: Macherey Nagel NucleoSpin DNA Stool kit

text: mixed culture metagenome

characteristic: organism

characteristic: project name

text: mixed culture metagenome

text: software

text: Oxford Nanopore, SQK-RAB20�C4

characteristic: sequencing method

text: 16S rRNA

text: BACMIX_minion_v1_9_20220720_macherey_nagel_MN3_bc06

characteristic: title


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF SZEGED

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

characteristic: collection date

text: ZymoBIOMICS Microbial Community Standard isolated by Qiagen QIAamp Fast DNA Stool Mini Kit

text: Hungary

text: 16S Barcoding Kit (SQK-RAB204)

characteristic: library construction method

text: ligation based

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

text: AAGCGTTGAAACCTTTGTCCTCTC

characteristic: multiplex identifiers

text: LongAmp Taq 2X Master Mix

characteristic: nucleic acid amplification

text: QIAamp Fast DNA Stool Mini Kit, Qiagen

characteristic: nucleic acid extraction

text: mixed culture metagenome

text: initial denaturation:95degC_1min; denaturation=95degC_20�Csec; annealing=55deg_30sec; extension=65deg_2min

characteristic: pcr conditions

characteristic: pcr primers

text: mixed culture metagenome

text: software

characteristic: sequence quality check

text: Oxford Nanopore, SQK-RAB20�C4

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: BACMIX_minion_v1_9_20220720_qiagen_Q1_bc10


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Csongrad county

characteristic: description

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: 16S Barcoding Kit (SQK-RAB204)

text: ligation based

text: Szeged

text: GTTTCATCTATCGGAGGGAATGGA

text: LongAmp Taq 2X Master Mix

text: QIAamp Fast DNA Stool Mini Kit, Qiagen

text: mixed culture metagenome

characteristic: organism

text: Comparative metagenomic profiling of canine gut microbial community using different sample preparation methodes

characteristic: project name

text: mixed culture metagenome

text: software

text: Oxford Nanopore, SQK-RAB20�C4

characteristic: sequencing method

text: 16S rRNA

text: BACMIX_minion_v1_9_20220720_qiagen_Q2_bc11

characteristic: title


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF SZEGED

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: 16SFw 5'-(ATCGCCTACCGTGAC - barcode - AGAGTTTGATCMTGGCTCAG); 16S Rev 5'-(ATCGCCTACCGTGAC - barcode - CGGTTACCTTGTTACGACTT)

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

characteristic: collection date

text: Hungary

text: 16S Barcoding Kit (SQK-RAB204)

characteristic: library construction method

text: ligation based

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

text: CAGGTAGAAAGAAGCAGAATCGGA

characteristic: multiplex identifiers

text: LongAmp Taq 2X Master Mix

characteristic: nucleic acid amplification

text: QIAamp Fast DNA Stool Mini Kit, Qiagen

characteristic: nucleic acid extraction

text: mixed culture metagenome

characteristic: pcr conditions

characteristic: pcr primers

text: mixed culture metagenome

text: software

characteristic: sequence quality check

text: Oxford Nanopore, SQK-RAB20�C4

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: BACMIX_minion_v1_9_20220720_qiagen_Q3_bc12


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Csongrad county

text: ZymoBIOMICS Microbial Community Standard isolated by Zymo Reserach Quick-DNA� HMW MagBead Kit

characteristic: description

text: ZymoBIOMICS Microbial Community DNA Standard

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: 16S Barcoding Kit (SQK-RAB204)

text: ligation based

text: Szeged

text: AAGAAAGTTGTCGGTGTCTTTGTG

text: LongAmp Taq 2X Master Mix

text: Quick-DNA HMW MagBead Kit, Zymo

text: mixed culture metagenome

characteristic: organism

characteristic: project name

text: mixed culture metagenome

text: software

text: Oxford Nanopore, SQK-RAB20�C4

characteristic: sequencing method

text: 16S rRNA

text: BACMIX_minion_v1_9_20220720_zymo_Z1_bc01

characteristic: title


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF SZEGED

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

characteristic: collection date

text: Hungary

text: 16S Barcoding Kit (SQK-RAB204)

characteristic: library construction method

text: ligation based

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

text: TCGATTCCGTTTGTAGTCGTCTGT

characteristic: multiplex identifiers

text: LongAmp Taq 2X Master Mix

characteristic: nucleic acid amplification

text: Quick-DNA HMW MagBead Kit, Zymo

characteristic: nucleic acid extraction

text: mixed culture metagenome

characteristic: pcr conditions

text: 27F 5'-(AGAGTTTGATCCTGGCTCAG); 1492R 5'-(GGTTACCTTGTTACGACTT)

characteristic: pcr primers

text: mixed culture metagenome

text: software

characteristic: sequence quality check

text: Oxford Nanopore, SQK-RAB20�C4

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: BACMIX_minion_v1_9_20220720_zymo_Z2_bc02


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Csongrad county

characteristic: description

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: 16S Barcoding Kit (SQK-RAB204)

text: ligation based

text: Szeged

text: GAGTCTTGTGTCCCAGTTACCAGG

text: LongAmp Taq 2X Master Mix

text: Quick-DNA HMW MagBead Kit, Zymo

text: mixed culture metagenome

characteristic: organism

characteristic: project name

text: mixed culture metagenome

text: software

text: Oxford Nanopore, SQK-RAB20�C4

characteristic: sequencing method

text: 16S rRNA

text: BACMIX_minion_v1_9_20220720_zymo_Z3_bc03

characteristic: title


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF SZEGED

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

characteristic: collection date

text: ZymoBIOMICS Microbial Community Standard isolated by Invitrogen PureLink Microbiome DNA Purification Kit

text: Hungary

text: 16S Barcoding Kit (SQK-RAB204)

characteristic: library construction method

text: ligation based

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

text: TTCTCGCAAAGGCAGAAAGTAGTC

characteristic: multiplex identifiers

text: LongAmp Taq 2X Master Mix

characteristic: nucleic acid amplification

text: PureLink Microbiome DNA Purification Kit, Invitrogen

characteristic: nucleic acid extraction

text: mixed culture metagenome

characteristic: pcr conditions

characteristic: pcr primers

text: mixed culture metagenome

text: software

characteristic: sequence quality check

text: Oxford Nanopore, SQK-RAB20�C4

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: BACMIX_minion_v1_9_20220825_invitrogen_I4_bc06


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Csongrad county

characteristic: description

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: 16S Barcoding Kit (SQK-RAB204)

text: ligation based

text: Szeged

text: GTGTTACCGTGGGAATGAATCCTT

text: LongAmp Taq 2X Master Mix

text: mixed culture metagenome

characteristic: organism

characteristic: project name

text: mixed culture metagenome

text: software

text: Oxford Nanopore, SQK-RAB20�C4

characteristic: sequencing method

text: 16S rRNA

text: BACMIX_minion_v1_9_20220825_invitrogen_I5_bc07

characteristic: title


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF SZEGED

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

characteristic: collection date

text: ZymoBIOMICS Microbial Community Standard isolated by Macherey-Nagel NucleoSpin DNA Stool Mini kit

text: Hungary

text: 16S Barcoding Kit (SQK-RAB204)

characteristic: library construction method

text: ligation based

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

text: GAGTCTTGTGTCCCAGTTACCAGG

characteristic: multiplex identifiers

text: LongAmp Taq 2X Master Mix

characteristic: nucleic acid amplification

text: Macherey Nagel NucleoSpin DNA Stool kit

characteristic: nucleic acid extraction

text: mixed culture metagenome

characteristic: pcr conditions

characteristic: pcr primers

text: mixed culture metagenome

text: software

characteristic: sequence quality check

text: Oxford Nanopore, SQK-RAB20�C4

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: BACMIX_minion_v1_9_20220825_macherey_nagel_MN4_bc03


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Csongrad county

characteristic: description

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: 16S Barcoding Kit (SQK-RAB204)

text: ligation based

text: Szeged

text: TTCGGATTCTATCGTGTTTCCCTA

text: LongAmp Taq 2X Master Mix

text: Macherey Nagel NucleoSpin DNA Stool kit

text: mixed culture metagenome

characteristic: organism

text: initial denaturation:95degC_1min; denaturation=95degC_20�Csec; annealing=55deg_30sec; extension=65deg_2min

characteristic: project name

text: mixed culture metagenome

text: software

text: Oxford Nanopore, SQK-RAB20�C4

characteristic: sequencing method

text: 16S rRNA

text: BACMIX_minion_v1_9_20220825_macherey_nagel_MN5_bc04

characteristic: title


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF SZEGED

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

characteristic: collection date

text: ZymoBIOMICS Microbial Community Standard isolated by Qiagen QIAamp Fast DNA Stool Mini Kit

text: Hungary

text: 16S Barcoding Kit (SQK-RAB204)

characteristic: library construction method

text: ligation based

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

text: TTCAGGGAACAAACCAAGTTACGT

characteristic: multiplex identifiers

text: LongAmp Taq 2X Master Mix

characteristic: nucleic acid amplification

text: QIAamp Fast DNA Stool Mini Kit, Qiagen

characteristic: nucleic acid extraction

text: mixed culture metagenome

characteristic: pcr conditions

characteristic: pcr primers

text: Comparative metagenomic profiling of canine gut microbial community using different sample preparation methodes

text: mixed culture metagenome

text: software

characteristic: sequence quality check

text: Oxford Nanopore, SQK-RAB20�C4

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: BACMIX_minion_v1_9_20220825_qiagen_Q4_bc08