characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: 16SFw 5'-(ATCGCCTACCGTGAC - barcode - AGAGTTTGATCMTGGCTCAG); 16S Rev 5'-(ATCGCCTACCGTGAC - barcode - CGGTTACCTTGTTACGACTT)

text: Csongrad county

characteristic: description

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: 16S Barcoding Kit (SQK-RAB204)

text: ligation based

text: Szeged

text: AACTAGGCACAGCGAGTCTTGGTT

text: LongAmp Taq 2X Master Mix

text: QIAamp Fast DNA Stool Mini Kit, Qiagen

text: mixed culture metagenome

characteristic: organism

characteristic: project name

text: mixed culture metagenome

text: software

text: Oxford Nanopore, SQK-RAB20�C4

characteristic: sequencing method

text: 16S rRNA

text: BACMIX_minion_v1_9_20220825_qiagen_Q5_bc09

characteristic: title


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

text: UNIVERSITY OF SZEGED

text: public

characteristic: SRA accession

tag: Namespace:UNIVERSITY OF SZEGED

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

characteristic: collection date

text: ZymoBIOMICS Microbial Community Standard isolated by Zymo Reserach Quick-DNA� HMW MagBead Kit

text: ZymoBIOMICS Microbial Community DNA Standard

text: Hungary

unit: DD

text: 16S Barcoding Kit (SQK-RAB204)

characteristic: library construction method

text: ligation based

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

text: AAGAAAGTTGTCGGTGTCTTTGTG

characteristic: multiplex identifiers

text: LongAmp Taq 2X Master Mix

characteristic: nucleic acid amplification

text: Quick-DNA HMW MagBead Kit, Zymo

characteristic: nucleic acid extraction

text: mixed culture metagenome

text: initial denaturation:95degC_1min; denaturation=95degC_20�Csec; annealing=55deg_30sec; extension=65deg_2min

characteristic: pcr conditions

text: 27F 5'-(AGAGTTTGATCCTGGCTCAG); 1492R 5'-(GGTTACCTTGTTACGACTT)

characteristic: pcr primers

text: Comparative metagenomic profiling of canine gut microbial community using different sample preparation methodes

text: mixed culture metagenome

text: software

characteristic: sequence quality check

text: Oxford Nanopore, SQK-RAB20�C4

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: BACMIX_minion_v1_9_20220825_zymo_Z4_bc01


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Csongrad county

characteristic: description

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: 16S Barcoding Kit (SQK-RAB204)

text: ligation based

text: Szeged

text: TCGATTCCGTTTGTAGTCGTCTGT

text: LongAmp Taq 2X Master Mix

text: Quick-DNA HMW MagBead Kit, Zymo

text: mixed culture metagenome

characteristic: organism

characteristic: project name

text: mixed culture metagenome

text: software

text: Oxford Nanopore, SQK-RAB20�C4

characteristic: sequencing method

text: 16S rRNA

text: BACMIX_minion_v1_9_20220825_zymo_Z5_bc02

characteristic: title


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF OXFORD

text: public

characteristic: SRA accession

tag: Namespace:UNIVERSITY OF OXFORD

characteristic: Submitter Id

text: University of Oxford

characteristic: collecting institution

characteristic: collection date

text: Modernising Medical Microbiology

characteristic: collector name

text: United Kingdom

text: human

characteristic: host common name

text: not collected

characteristic: host health state

text: Homo sapien

characteristic: host scientific name

text: not collected

characteristic: host sex

text: not collected

characteristic: host subject id

text: RT-PCR

characteristic: influenza test method

text: P

characteristic: influenza test result

text: B

characteristic: influenza virus type

text: Influenza B virus

text: not applicable

characteristic: other pathogens test result

characteristic: other pathogens tested

text: Influenza B virus

text: fluB

characteristic: serotype (required for a seropositive sample)

text: Influenza genome sequencing


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-06-07-2023-10:14:15:070-21445

tag: Namespace:Hospital General Universitario Gregorio Maranon

text: HGUGM

text: HGUGM

text: SARS-CoV-2 virus sequencing Illumina

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: diseased

text: Human

text: male

characteristic: isolate

text: Severe acute respiratory syndrome coronavirus 2

characteristic: organism

characteristic: title


text: Hospital General Universitario Gregorio Maranon

text: public

characteristic: SRA accession

text: ena-SAMPLE-TAB-06-07-2023-10:14:15:071-21446

characteristic: Submitter Id

text: HGUGM

characteristic: collecting institution

characteristic: collection date

text: HGUGM

characteristic: collector name

text: SARS-CoV-2 virus sequencing Illumina

text: Spain

text: Human

characteristic: host common name

text: diseased

characteristic: host health state

text: Human

characteristic: host scientific name

text: male

characteristic: host sex

characteristic: host subject id


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ena-SAMPLE-TAB-06-07-2023-10:14:15:071-21447

text: HGUGM

text: HGUGM

text: SARS-CoV-2 virus sequencing Illumina

text: Spain

text: Human

text: diseased

text: Human

text: male


text: UNIVERSITY OF SZEGED

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Can_fam_Toti_illumina_v1_2_20220525_invitrogen_I1_R1

text: Quick-16S-AdapterFw-(TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG), Quick-16S-AdapterRev-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG and Illumina P5 5'-(AATGATACGGCGACCACCGA), P7 5'-(CAAGCAGAAGACGGCATACGAGAT)

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

unit: years

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

text: male

text: cross-breeding of Hungarian Puli with French and German herding dogs originated from 17. century

characteristic: host subspecific genetic lineage

characteristic: host taxid

text: Quick-16S NGS Library Prep Kit V1-V2

characteristic: library construction method

text: PCR

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

text: 3-(ACCTGGAT), 5-(TTCTAGAC)

characteristic: multiplex identifiers

text: Quick-16S NGS Library Prep Kit V1-V2

characteristic: nucleic acid amplification

text: PureLink Microbiome DNA Purification Kit, Invitrogen

characteristic: nucleic acid extraction

text: gut metagenome

characteristic: organism

text: initial denaturation:95degC_10min; denaturation=95degC_30sec; annealing=55deg_30sec; extension=72deg_3min

characteristic: pcr conditions

text: Quick-16S Primer Set V1-V2

characteristic: pcr primers

characteristic: project name

text: gut metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq v2 500 nano

characteristic: sequencing method

characteristic: target gene

characteristic: target subfragment

text: toti_illumina_v1_2_20220525_invitrogen_I1_R1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Can_fam_Toti_illumina_v1_2_20220525_invitrogen_I2_R1

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

characteristic: host scientific name

text: male

characteristic: host sex

characteristic: host subspecific genetic lineage

text: Quick-16S NGS Library Prep Kit V1-V2

text: PCR

text: Szeged

text: 3-(GTGCCATA), 5-(CCGATCTT)

text: Quick-16S NGS Library Prep Kit V1-V2

text: gut metagenome

text: Quick-16S Primer Set V1-V2

text: gut metagenome

text: software

text: Illumina MiSeq v2 500 nano

text: toti_illumina_v1_2_20220525_invitrogen_I2_R1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Can_fam_Toti_illumina_v1_2_20220525_invitrogen_I3_R1

characteristic: Submitter Id

text: Csongrad county

text: no

text: Hungary

text: stool

text: Hungarian herding terrier: Pumi

text: omnivore

text: Harsanyi-Tekergo Bator

text: Canis lupus familiaris

text: male

characteristic: host taxid

text: Quick-16S NGS Library Prep Kit V1-V2

characteristic: library construction method

text: PCR

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

text: 3-(TGAATCCG), 5-(TAAGATCC)

characteristic: multiplex identifiers

text: Quick-16S NGS Library Prep Kit V1-V2

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: gut metagenome

characteristic: organism

characteristic: pcr conditions

text: Quick-16S Primer Set V1-V2

characteristic: pcr primers

characteristic: project name

text: gut metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq v2 500 nano

characteristic: sequencing method

characteristic: target gene

characteristic: target subfragment

text: toti_illumina_v1_2_20220525_invitrogen_I3_R1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Can_fam_Toti_illumina_v1_2_20220525_invitrogen_I4_R1

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

characteristic: host scientific name

text: male

characteristic: host sex

characteristic: host subspecific genetic lineage

text: Quick-16S NGS Library Prep Kit V1-V2

text: PCR

text: Szeged

text: 3-(CATGATGC), 5-(AGGTCATT)

text: Quick-16S NGS Library Prep Kit V1-V2

text: gut metagenome

text: Quick-16S Primer Set V1-V2

text: Comparative metagenomic profiling of canine gut microbial community using different sample preparation methodes

text: gut metagenome

text: software

text: Illumina MiSeq v2 500 nano

text: toti_illumina_v1_2_20220525_invitrogen_I4_R1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Can_fam_Toti_illumina_v1_2_20220525_macherey_nagel_MN1_R1

characteristic: Submitter Id

text: Csongrad county

text: no

text: Hungary

text: stool

text: Hungarian herding terrier: Pumi

text: omnivore

text: Harsanyi-Tekergo Bator

text: Canis lupus familiaris

text: male

characteristic: host taxid

text: Quick-16S NGS Library Prep Kit V1-V2

characteristic: library construction method

text: PCR

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

text: 3-(AATGTCCT), 5-(TTCTAGAC)

characteristic: multiplex identifiers

text: Quick-16S NGS Library Prep Kit V1-V2

characteristic: nucleic acid amplification

text: Macherey Nagel NucleoSpin DNA Stool kit

characteristic: nucleic acid extraction

text: gut metagenome

characteristic: organism

characteristic: pcr conditions

text: Quick-16S Primer Set V1-V2

characteristic: pcr primers

characteristic: project name

text: gut metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq v2 500 nano

characteristic: sequencing method

characteristic: target gene

characteristic: target subfragment

text: toti_illumina_v1_2_20220525_macherey_nagel_MN1_R1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Can_fam_Toti_illumina_v1_2_20220525_macherey_nagel_MN2_R1

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

characteristic: host scientific name

text: male

characteristic: host sex

characteristic: host subspecific genetic lineage

text: Quick-16S NGS Library Prep Kit V1-V2

text: PCR

text: Szeged

text: 3-(ATAGGCTC), 5-(CCGATCTT)

text: Quick-16S NGS Library Prep Kit V1-V2

text: Macherey Nagel NucleoSpin DNA Stool kit

text: gut metagenome

text: Quick-16S Primer Set V1-V2

text: gut metagenome

text: software

text: Illumina MiSeq v2 500 nano

text: toti_illumina_v1_2_20220525_macherey_nagel_MN2_R1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Can_fam_Toti_illumina_v1_2_20220525_macherey_nagel_MN3_R1

characteristic: Submitter Id

text: Csongrad county

text: no

text: Hungary

text: stool

text: Hungarian herding terrier: Pumi

text: omnivore

text: Harsanyi-Tekergo Bator

text: Canis lupus familiaris

text: male

characteristic: host taxid

text: Quick-16S NGS Library Prep Kit V1-V2

characteristic: library construction method

text: PCR

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

text: 3-(ACCTGGAT), 5-(TAAGATCC)

characteristic: multiplex identifiers

text: Quick-16S NGS Library Prep Kit V1-V2

characteristic: nucleic acid amplification

text: Macherey Nagel NucleoSpin DNA Stool kit

characteristic: nucleic acid extraction

text: gut metagenome

characteristic: organism

characteristic: pcr conditions

text: Quick-16S Primer Set V1-V2

characteristic: pcr primers

characteristic: project name

text: gut metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq v2 500 nano

characteristic: sequencing method

characteristic: target gene

characteristic: target subfragment

text: toti_illumina_v1_2_20220525_macherey_nagel_MN3_R1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Can_fam_Toti_illumina_v1_2_20220525_macherey_nagel_MN4_R1

characteristic: Submitter Id

text: Quick-16S-AdapterFw-(TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG), Quick-16S-AdapterRev-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG and Illumina P5 5'-(AATGATACGGCGACCACCGA), P7 5'-(CAAGCAGAAGACGGCATACGAGAT)

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

characteristic: host scientific name

text: male

characteristic: host sex

text: cross-breeding of Hungarian Puli with French and German herding dogs originated from 17. century

characteristic: host subspecific genetic lineage

text: Quick-16S NGS Library Prep Kit V1-V2

text: PCR

text: Szeged

text: 3-(GTGCCATA), 5-(AGGTCATT)

text: Quick-16S NGS Library Prep Kit V1-V2

text: Macherey Nagel NucleoSpin DNA Stool kit

text: gut metagenome

text: Quick-16S Primer Set V1-V2

text: gut metagenome

text: software

text: Illumina MiSeq v2 500 nano

text: toti_illumina_v1_2_20220525_macherey_nagel_MN4_R1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Can_fam_Toti_illumina_v1_2_20220525_zymo_Z1_R1

characteristic: Submitter Id

text: Csongrad county

text: no

text: Hungary

text: stool

text: Hungarian herding terrier: Pumi

text: omnivore

text: Harsanyi-Tekergo Bator

text: Canis lupus familiaris

text: male

characteristic: host taxid

text: Quick-16S NGS Library Prep Kit V1-V2

characteristic: library construction method

text: PCR

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

text: 3-(TGAATCCG), 5-(TTCTAGAC)

characteristic: multiplex identifiers

text: Quick-16S NGS Library Prep Kit V1-V2

characteristic: nucleic acid amplification

text: Quick-DNA HMW MagBead Kit, Zymo

characteristic: nucleic acid extraction

text: gut metagenome

characteristic: organism

text: initial denaturation:95degC_10min; denaturation=95degC_30sec; annealing=55deg_30sec; extension=72deg_3min

characteristic: pcr conditions

text: Quick-16S Primer Set V1-V2

characteristic: pcr primers

characteristic: project name

text: gut metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq v2 500 nano

characteristic: sequencing method

characteristic: target gene

characteristic: target subfragment

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Can_fam_Toti_illumina_v1_2_20220525_zymo_Z2_R1

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

characteristic: host scientific name

text: male

characteristic: host sex

characteristic: host subspecific genetic lineage

text: Quick-16S NGS Library Prep Kit V1-V2

text: PCR

text: Szeged

text: 3-(CATGATGC), 5-(CCGATCTT)

text: Quick-16S NGS Library Prep Kit V1-V2

text: Quick-DNA HMW MagBead Kit, Zymo

text: gut metagenome

text: Quick-16S Primer Set V1-V2

text: gut metagenome

text: software

text: Illumina MiSeq v2 500 nano


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Can_fam_Toti_illumina_v1_2_20220525_zymo_Z3_R1

characteristic: Submitter Id

text: Csongrad county

text: no

text: Hungary

text: stool

text: Hungarian herding terrier: Pumi

text: omnivore

text: Harsanyi-Tekergo Bator

text: Canis lupus familiaris

text: male

characteristic: host taxid

text: Quick-16S NGS Library Prep Kit V1-V2

characteristic: library construction method

text: PCR

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

text: 3-(AATGTCCT), 5-(TAAGATCC)

characteristic: multiplex identifiers

text: Quick-16S NGS Library Prep Kit V1-V2

characteristic: nucleic acid amplification

text: Quick-DNA HMW MagBead Kit, Zymo

characteristic: nucleic acid extraction

text: gut metagenome

characteristic: organism

characteristic: pcr conditions

text: Quick-16S Primer Set V1-V2

characteristic: pcr primers

characteristic: project name

text: gut metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq v2 500 nano

characteristic: sequencing method

characteristic: target gene

characteristic: target subfragment

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Can_fam_Toti_illumina_v1_2_20220525_zymo_Z4_R1

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

characteristic: host scientific name

text: male

characteristic: host sex

characteristic: host subspecific genetic lineage

text: Quick-16S NGS Library Prep Kit V1-V2

text: PCR

text: Szeged

text: 3-(ATAGGCTC), 5-(AGGTCATT)

text: Quick-16S NGS Library Prep Kit V1-V2

text: Quick-DNA HMW MagBead Kit, Zymo

text: gut metagenome

text: Quick-16S Primer Set V1-V2

text: gut metagenome

text: software

text: Illumina MiSeq v2 500 nano


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Can_fam_Toti_illumina_v1_3_20211204_invitrogen_I1_R1

characteristic: Submitter Id

text: Perkin-Fw-(AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT), Perkin-Rev-(CAAGCAGAAGACGGCATACGAGATXXXXXXXXXXXX1GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT)

text: Csongrad county

text: no

text: Hungary

text: stool

text: Hungarian herding terrier: Pumi

text: omnivore

text: Harsanyi-Tekergo Bator

text: Canis lupus familiaris

text: male

characteristic: host taxid

text: NEXTflex 16S V1 - V3 Amplicon-Seq Kit - 12

characteristic: library construction method

text: PCR

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

text: CCGTCTCTCCGG

characteristic: multiplex identifiers

characteristic: nucleic acid amplification

text: PureLink Microbiome DNA Purification Kit, Invitrogen

characteristic: nucleic acid extraction

text: gut metagenome

characteristic: organism

text: initial denaturation:98degC_4min; denaturation=98degC_30sec; annealing=60deg_30sec; extension=72deg_20sec

characteristic: pcr conditions

text: 16S-V1-V3-Fw(CTCTTTCCCTACACGACGCTCTTCCGATCTAGAGTTTGATCCTGGCTCAG), 16S-V1-V3-Rev(CTGGAGTTCAGACGTGTGCTCTTCCGATCTGTATTACCGCGGCTGCTGG)

characteristic: pcr primers

text: Comparative metagenomic profiling of canine gut microbial community using different sample preparation methodes

characteristic: project name

text: gut metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq v2 500 nano

characteristic: sequencing method

characteristic: target gene

characteristic: target subfragment

text: toti_illumina_v1_3_20211204_invitrogen_I1_R1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Can_fam_Toti_illumina_v1_3_20211204_invitrogen_I2_R1

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

characteristic: host scientific name

text: male

characteristic: host sex

characteristic: host subspecific genetic lineage

text: PCR

text: Szeged

text: ACGCTCTTCCGG

text: gut metagenome

text: gut metagenome

text: software

text: Illumina MiSeq v2 500 nano

text: toti_illumina_v1_3_20211204_invitrogen_I2_R1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Can_fam_Toti_illumina_v1_3_20211204_macherey_nagel_MN1_R1

characteristic: Submitter Id

text: Csongrad county

text: no

text: Hungary

text: stool

text: Hungarian herding terrier: Pumi

text: omnivore

text: Harsanyi-Tekergo Bator

text: Canis lupus familiaris

text: male

text: cross-breeding of Hungarian Puli with French and German herding dogs originated from 17. century

characteristic: host taxid

characteristic: library construction method

text: PCR

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

text: TTATCAGTCCTT

characteristic: multiplex identifiers

characteristic: nucleic acid amplification

text: Macherey Nagel NucleoSpin DNA Stool kit

characteristic: nucleic acid extraction

text: gut metagenome

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: gut metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq v2 500 nano

characteristic: sequencing method

characteristic: target gene

characteristic: target subfragment

text: toti_illumina_v1_3_20211204_macherey_nagel_MN1_R1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Can_fam_Toti_illumina_v1_3_20211204_macherey_nagel_MN2_R1

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

characteristic: host scientific name

text: male

characteristic: host sex

characteristic: host subspecific genetic lineage

text: PCR

text: Szeged

text: GTCATCGCGTCC

text: Macherey Nagel NucleoSpin DNA Stool kit

text: gut metagenome

text: gut metagenome

text: software

text: Illumina MiSeq v2 500 nano

text: toti_illumina_v1_3_20211204_macherey_nagel_MN2_R1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Can_fam_Toti_illumina_v1_3_20211204_qiagen_Q1_R1

characteristic: Submitter Id

text: Csongrad county

text: no

text: Hungary

text: stool

text: Hungarian herding terrier: Pumi

text: omnivore

text: Harsanyi-Tekergo Bator

text: Canis lupus familiaris

text: male

characteristic: host taxid

characteristic: library construction method

text: PCR

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

text: AAGCGTACGTCC

characteristic: multiplex identifiers

characteristic: nucleic acid amplification

text: QIAamp Fast DNA Stool Mini Kit, Qiagen

characteristic: nucleic acid extraction

text: gut metagenome

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: gut metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq v2 500 nano

characteristic: sequencing method

characteristic: target gene

characteristic: target subfragment

text: toti_illumina_v1_3_20211204_qiagen_Q1_R1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Can_fam_Toti_illumina_v1_3_20211204_qiagen_Q2_R1

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

characteristic: host scientific name

text: male

characteristic: host sex

characteristic: host subspecific genetic lineage

text: PCR

text: Szeged

text: TCGGGAAGGTCC

text: QIAamp Fast DNA Stool Mini Kit, Qiagen

text: gut metagenome

text: gut metagenome

text: software

text: Illumina MiSeq v2 500 nano

text: toti_illumina_v1_3_20211204_qiagen_Q2_R1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Can_fam_Toti_illumina_v1_3_20211216_invitrogen_I3_R1

characteristic: Submitter Id

text: Perkin-Fw-(AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT), Perkin-Rev-(CAAGCAGAAGACGGCATACGAGATXXXXXXXXXXXX1GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT)

text: Csongrad county

text: no

text: Hungary

text: stool

text: Hungarian herding terrier: Pumi

text: omnivore

text: Harsanyi-Tekergo Bator

text: Canis lupus familiaris

text: male

characteristic: host taxid

text: NEXTflex 16S V1 - V3 Amplicon-Seq Kit - 12

characteristic: library construction method

text: PCR

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

text: AAATCTCAGGCC

characteristic: multiplex identifiers

characteristic: nucleic acid amplification

characteristic: nucleic acid extraction

text: gut metagenome

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: gut metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq v2 500 nano

characteristic: sequencing method

characteristic: target gene

characteristic: target subfragment

text: toti_illumina_v1_3_20211216_invitrogen_I3_R1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Can_fam_Toti_illumina_v1_3_20211216_invitrogen_I4_R1

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

characteristic: host scientific name

text: male

characteristic: host sex

characteristic: host subspecific genetic lineage

text: PCR

text: Szeged

text: GTGCGCGGCCGG

text: PureLink Microbiome DNA Purification Kit, Invitrogen

text: gut metagenome

text: initial denaturation:98degC_4min; denaturation=98degC_30sec; annealing=60deg_30sec; extension=72deg_20sec

text: 16S-V1-V3-Fw(CTCTTTCCCTACACGACGCTCTTCCGATCTAGAGTTTGATCCTGGCTCAG), 16S-V1-V3-Rev(CTGGAGTTCAGACGTGTGCTCTTCCGATCTGTATTACCGCGGCTGCTGG)

text: Comparative metagenomic profiling of canine gut microbial community using different sample preparation methodes

text: gut metagenome

text: software

text: Illumina MiSeq v2 500 nano

text: toti_illumina_v1_3_20211216_invitrogen_I4_R1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Can_fam_Toti_illumina_v1_3_20211216_macherey_nagel_MN3_R1

characteristic: Submitter Id

text: Csongrad county

text: no

text: Hungary

text: stool

text: Hungarian herding terrier: Pumi

text: omnivore

text: Harsanyi-Tekergo Bator

text: Canis lupus familiaris

text: male

text: cross-breeding of Hungarian Puli with French and German herding dogs originated from 17. century

characteristic: host taxid

characteristic: library construction method

text: PCR

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

text: AATAATTGGTCC

characteristic: multiplex identifiers

characteristic: nucleic acid amplification

text: Macherey Nagel NucleoSpin DNA Stool kit

characteristic: nucleic acid extraction

text: gut metagenome

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: gut metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq v2 500 nano

characteristic: sequencing method

characteristic: target gene

characteristic: target subfragment

text: toti_illumina_v1_3_20211216_macherey_nagel_MN3_R1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Can_fam_Toti_illumina_v1_3_20211216_macherey_nagel_MN4_R1

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

characteristic: host scientific name

text: male

characteristic: host sex

characteristic: host subspecific genetic lineage

text: PCR

text: Szeged

text: GTCGTCAACCGG

text: Macherey Nagel NucleoSpin DNA Stool kit

text: gut metagenome

text: gut metagenome

text: software

text: Illumina MiSeq v2 500 nano

text: toti_illumina_v1_3_20211216_macherey_nagel_MN4_R1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Can_fam_Toti_illumina_v1_3_20211216_qiagen_Q1_R1

characteristic: Submitter Id

text: Csongrad county

text: no

text: Hungary

text: stool

text: Hungarian herding terrier: Pumi

text: omnivore

text: Harsanyi-Tekergo Bator

text: Canis lupus familiaris

text: male

characteristic: host taxid

characteristic: library construction method

text: PCR

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

text: TGACGCGCGGCC

characteristic: multiplex identifiers

characteristic: nucleic acid amplification

text: QIAamp Fast DNA Stool Mini Kit, Qiagen

characteristic: nucleic acid extraction

text: gut metagenome

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: gut metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq v2 500 nano

characteristic: sequencing method

characteristic: target gene

characteristic: target subfragment

text: toti_illumina_v1_3_20211216_qiagen_Q1_R1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Can_fam_Toti_illumina_v1_3_20211216_qiagen_Q4_R1

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

characteristic: host scientific name

text: male

characteristic: host sex

characteristic: host subspecific genetic lineage

text: PCR

text: Szeged

text: GAGGCATCGGCC

text: QIAamp Fast DNA Stool Mini Kit, Qiagen

text: gut metagenome

text: gut metagenome

text: software

text: Illumina MiSeq v2 500 nano

text: toti_illumina_v1_3_20211216_qiagen_Q4_R1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Can_fam_Toti_illumina_v1_3_20211216_zymo_Z1_R1

characteristic: Submitter Id

text: Perkin-Fw-(AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT), Perkin-Rev-(CAAGCAGAAGACGGCATACGAGATXXXXXXXXXXXX1GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT)

text: Csongrad county

text: no

text: Hungary

text: stool

text: Hungarian herding terrier: Pumi

text: omnivore

text: Harsanyi-Tekergo Bator

text: Canis lupus familiaris

text: male

characteristic: host taxid

text: NEXTflex 16S V1 - V3 Amplicon-Seq Kit - 12

characteristic: library construction method

text: PCR

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

text: AAGCGTACGTCC

characteristic: multiplex identifiers

characteristic: nucleic acid amplification

text: Quick-DNA HMW MagBead Kit, Zymo

characteristic: nucleic acid extraction

text: gut metagenome

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: gut metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq v2 500 nano

characteristic: sequencing method

characteristic: target gene

characteristic: target subfragment

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Can_fam_Toti_illumina_v1_3_20211216_zymo_Z2_R1

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

characteristic: host scientific name

text: male

characteristic: host sex

characteristic: host subspecific genetic lineage

text: PCR

text: Szeged

text: TTATCAGTCCTT

text: Quick-DNA HMW MagBead Kit, Zymo

text: gut metagenome

text: gut metagenome

text: software

text: Illumina MiSeq v2 500 nano


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Can_fam_Toti_illumina_v1_3_20220117_invitrogen_I3_R1

characteristic: Submitter Id

text: Csongrad county

text: no

text: Hungary

text: stool

text: Hungarian herding terrier: Pumi

text: omnivore

text: Harsanyi-Tekergo Bator

text: Canis lupus familiaris

text: male

characteristic: host taxid

characteristic: library construction method

text: PCR

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

text: AAATCTCAGGCC

characteristic: multiplex identifiers

characteristic: nucleic acid amplification

text: PureLink Microbiome DNA Purification Kit, Invitrogen

characteristic: nucleic acid extraction

text: gut metagenome

characteristic: organism

text: initial denaturation:98degC_4min; denaturation=98degC_30sec; annealing=60deg_30sec; extension=72deg_20sec

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: gut metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq v2 300 nano

characteristic: sequencing method

characteristic: target gene

characteristic: target subfragment

text: toti_illumina_v1_3_20220117_invitrogen_I3_R1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Can_fam_Toti_illumina_v1_3_20220117_invitrogen_I4_R1

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

characteristic: host scientific name

text: male

characteristic: host sex

characteristic: host subspecific genetic lineage

text: PCR

text: Szeged

text: GTGCGCGGCCGG

text: gut metagenome

text: 16S-V1-V3-Fw(CTCTTTCCCTACACGACGCTCTTCCGATCTAGAGTTTGATCCTGGCTCAG), 16S-V1-V3-Rev(CTGGAGTTCAGACGTGTGCTCTTCCGATCTGTATTACCGCGGCTGCTGG)

text: Comparative metagenomic profiling of canine gut microbial community using different sample preparation methodes

text: gut metagenome

text: software

text: Illumina MiSeq v2 300 nano

text: toti_illumina_v1_3_20220117_invitrogen_I4_R1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Can_fam_Toti_illumina_v1_3_20220117_macherey_nagel_MN1_R1

characteristic: Submitter Id

text: Csongrad county

text: no

text: Hungary

text: stool

text: Hungarian herding terrier: Pumi

text: omnivore

text: Harsanyi-Tekergo Bator

text: Canis lupus familiaris

text: male

text: cross-breeding of Hungarian Puli with French and German herding dogs originated from 17. century

characteristic: host taxid

characteristic: library construction method

text: PCR

characteristic: library screening strategy

text: Szeged

characteristic: local environmental context

text: TTATCAGTCCTT

characteristic: multiplex identifiers

characteristic: nucleic acid amplification

text: Macherey Nagel NucleoSpin DNA Stool kit

characteristic: nucleic acid extraction

text: gut metagenome

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

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text: gut metagenome

text: software

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text: Illumina MiSeq v2 300 nano

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characteristic: title


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characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

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characteristic: INSDC center name

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characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Can_fam_Toti_illumina_v1_3_20220117_macherey_nagel_MN4_R1

characteristic: Submitter Id

characteristic: adapters

text: Csongrad county

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

characteristic: host scientific name

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text: Macherey Nagel NucleoSpin DNA Stool kit

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text: Illumina MiSeq v2 300 nano

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characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

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text: public

characteristic: INSDC status

characteristic: SRA accession

text: Can_fam_Toti_illumina_v1_3_20220117_qiagen_Q2_R1

characteristic: Submitter Id

text: Csongrad county

text: no

text: Hungary

text: stool

text: Hungarian herding terrier: Pumi

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text: Harsanyi-Tekergo Bator

text: Canis lupus familiaris

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characteristic: host taxid

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text: QIAamp Fast DNA Stool Mini Kit, Qiagen

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text: public

characteristic: INSDC status

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text: no

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text: Hungary

characteristic: geographic location (country and/or sea)

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characteristic: host age

text: stool

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text: Hungarian herding terrier: Pumi

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text: NEXTflex 16S V1 - V3 Amplicon-Seq Kit - 12

text: PCR

text: Szeged

text: CCGTCTCTCCGG

text: Quick-DNA HMW MagBead Kit, Zymo

text: gut metagenome

text: gut metagenome

text: software

text: Illumina MiSeq v2 300 nano


characteristic: ENA-CHECKLIST

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characteristic: ENA-LAST-UPDATE

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text: public

characteristic: INSDC status

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text: Csongrad county

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text: Hungarian herding terrier: Pumi

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text: Harsanyi-Tekergo Bator

text: Canis lupus familiaris

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characteristic: host taxid

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text: Quick-DNA HMW MagBead Kit, Zymo

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characteristic: pcr conditions

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characteristic: project name

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text: software

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characteristic: ENA-LAST-UPDATE

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text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

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characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

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text: Csongrad county

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characteristic: collection date

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text: no

characteristic: environmental medium

text: Hungary

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characteristic: host age

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text: toti_illumina_v1_3_20220126_invitrogen_I3_R1


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text: public

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characteristic: SRA accession

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text: Csongrad county

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characteristic: title


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text: UNIVERSITY OF SZEGED

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text: public

characteristic: INSDC status

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text: Can_fam_Toti_illumina_v1_3_20220126_qiagen_Q1_R1

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text: Csongrad county

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characteristic: collection date

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

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characteristic: host age

text: stool

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text: QIAamp Fast DNA Stool Mini Kit, Qiagen

text: gut metagenome

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text: toti_illumina_v1_3_20220126_qiagen_Q1_R1


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text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

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text: public

characteristic: INSDC status

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text: Csongrad county

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text: Harsanyi-Tekergo Bator

text: Canis lupus familiaris

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text: QIAamp Fast DNA Stool Mini Kit, Qiagen

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text: gut metagenome

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text: no

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text: Hungary

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text: stool

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text: Hungarian herding terrier: Pumi

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text: QIAamp Fast DNA Stool Mini Kit, Qiagen

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text: public

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text: Harsanyi-Tekergo Bator

text: Canis lupus familiaris

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text: Quick-DNA HMW MagBead Kit, Zymo

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text: public

characteristic: INSDC status

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text: Can_fam_Toti_illumina_v1_3_20221004_invitrogen_I4_R1

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text: Csongrad county

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characteristic: collection date

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

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characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

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text: omnivore

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text: Harsanyi-Tekergo Bator

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text: PCR

text: Szeged

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text: software

text: Illumina MiSeq v2 500 nano

text: toti_illumina_v1_3_20221004_invitrogen_I4_R1


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characteristic: External Id

text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

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text: public

characteristic: INSDC status

characteristic: SRA accession

text: Can_fam_Toti_illumina_v1_3_20221004_macherey_nagel_MN3_R1

characteristic: Submitter Id

text: Csongrad county

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text: Hungarian herding terrier: Pumi

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text: Harsanyi-Tekergo Bator

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text: gut metagenome

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text: UNIVERSITY OF SZEGED

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text: public

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text: Csongrad county

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

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text: Hungarian herding terrier: Pumi

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text: PCR

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text: QIAamp Fast DNA Stool Mini Kit, Qiagen

text: gut metagenome

text: 16S-V1-V3-Fw(CTCTTTCCCTACACGACGCTCTTCCGATCTAGAGTTTGATCCTGGCTCAG), 16S-V1-V3-Rev(CTGGAGTTCAGACGTGTGCTCTTCCGATCTGTATTACCGCGGCTGCTGG)

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text: Illumina MiSeq v2 500 nano

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text: public

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text: Can_fam_Toti_illumina_v1_3_20221004_qiagen_Q4_R1

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text: Csongrad county

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text: public

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characteristic: Submitter Id

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text: Csongrad county

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: description

text: no

characteristic: environmental medium

text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

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characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

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characteristic: host scientific name

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text: PCR

text: Szeged

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text: Illumina MiSeq v2 500 nano


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text: public

characteristic: INSDC status

characteristic: SRA accession

text: Can_fam_Toti_illumina_v1_3_20221004_zymo_Z4_R1

characteristic: Submitter Id

text: Csongrad county

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text: Harsanyi-Tekergo Bator

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text: Quick-DNA HMW MagBead Kit, Zymo

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text: UNIVERSITY OF SZEGED

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characteristic: SRA accession

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characteristic: Submitter Id

text: Quick-16S-AdapterFw-(TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG), Quick-16S-AdapterRev-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG and Illumina P5 5'-(AATGATACGGCGACCACCGA), P7 5'-(CAAGCAGAAGACGGCATACGAGAT)

characteristic: adapters

text: Csongrad county

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characteristic: collection date

characteristic: description

text: no

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text: Hungary

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host age

text: stool

characteristic: host body product

text: Hungarian herding terrier: Pumi

characteristic: host common name

text: omnivore

characteristic: host diet

text: Harsanyi-Tekergo Bator

characteristic: host family relationship

text: Canis lupus familiaris

characteristic: host scientific name

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text: Quick-16S NGS Library Prep Kit V3-V4

text: PCR

text: Szeged

text: 3-(ACCTGGAT), 5-(TTCTAGAC)

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