text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Daycare 1, Sample 1A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Daycare 1, Sample 1A


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Daycare 1, Sample 1B

characteristic: description

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: dust metagenome

characteristic: organism

text: dust metagenome

text: Daycare 1, Sample 1B

characteristic: title


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Daycare 1, Sample 2A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Daycare 1, Sample 2A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Daycare 1, Sample 2B

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Daycare 1, Sample 2B


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Daycare 1, Sample 3A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Daycare 1, Sample 3A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Daycare 1, Sample 3B

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Daycare 1, Sample 3B


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Daycare 1, Sample 4A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Daycare 1, Sample 4A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Daycare 1, Sample 4B

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Daycare 1, Sample 4B


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Daycare 1, Sample 5A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Daycare 1, Sample 5A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Daycare 1, Sample 5B

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Daycare 1, Sample 5B


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Daycare 2, Sample 1A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Daycare 2, Sample 1A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Daycare 2, Sample 1B

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Daycare 2, Sample 1B


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 3, Sample 1A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 3, Sample 1A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 3, Sample 1B

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 3, Sample 1B


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Daycare 3, Sample 1A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Daycare 3, Sample 1A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Daycare 3, Sample 1B

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Daycare 3, Sample 1B


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Daycare 3, Sample 2A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Daycare 3, Sample 2A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Daycare 3, Sample 2B

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Daycare 3, Sample 2B


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Daycare 3, Sample 3A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Daycare 3, Sample 3A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Daycare 3, Sample 3B

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Daycare 3, Sample 3B


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Daycare 3, Sample 4A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Daycare 3, Sample 4A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Daycare 3, Sample 4B

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Daycare 3, Sample 4B


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Daycare 3, Sample 5A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Daycare 3, Sample 5A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Daycare 3, Sample 5B

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Daycare 3, Sample 5B


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 4, Sample 1A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 4, Sample 1A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 4, Sample 1B

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 4, Sample 1B


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 5, Sample 1A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 5, Sample 1A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 5, Sample 1B

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 5, Sample 1B


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 6, Sample 1A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 6, Sample 1A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 6, Sample 1B

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 6, Sample 1B


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 7, Sample 1A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 7, Sample 1A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 7, Sample 1B

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 7, Sample 1B


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 8, Sample 1A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 8, Sample 1A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 8, Sample 1B

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 8, Sample 1B


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 9, Sample 1A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 9, Sample 1A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 9, Sample 1B

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 9, Sample 1B


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 10, Sample 1A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 10, Sample 1A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 10, Sample 1B

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 10, Sample 1B


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 11, Sample 1A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 11, Sample 1A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 11, Sample 1B

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 11, Sample 1B


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 12, Sample 1A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 12, Sample 1A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 12, Sample 1B

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 12, Sample 1B


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 13, Sample 1A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 13, Sample 1A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 13, Sample 1B

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 13, Sample 1B


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 14, Sample 1A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 14, Sample 1A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 14, Sample 1B

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 14, Sample 1B


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 15, Sample 1A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 15, Sample 1A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 15, Sample 1B

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 15, Sample 1B


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 16, Sample 1A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 16, Sample 1A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 16, Sample 1B

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 16, Sample 1B


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 17, Sample 1A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 17, Sample 1A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 18, Sample 1A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 18, Sample 1A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 18, Sample 1B

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 18, Sample 1B


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 19, Sample 1A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 19, Sample 1A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 19, Sample 1B

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 19, Sample 1B


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 20, Sample 1A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 20, Sample 1A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 20, Sample 1B

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 20, Sample 1B


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 21, Sample 1A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 21, Sample 1A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 21, Sample 1B

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 21, Sample 1B


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 22, Sample 1A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 22, Sample 1A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 22, Sample 1B

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 22, Sample 1B


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Negative Extraction Control 1A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Negative Extraction Control 1A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Negative Extraction Control 1B

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Negative Extraction Control 1B


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Negative Extraction Control 2A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Negative Extraction Control 2A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Negative Extraction Control 2B

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Negative Extraction Control 2B


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Negative Extraction Control 3A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Negative Extraction Control 3A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Negative Extraction Control 3B

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Negative Extraction Control 3B


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 23, Sample 1A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 23, Sample 1A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Home 24, Sample 1A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Home 24, Sample 1A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Daycare 2, Sample 2A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Daycare 2, Sample 2A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Negative Extraction Control 4A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Negative Extraction Control 4A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Negative Sequencing Control 1A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Negative Sequencing Control 1A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Positive Sequencing Control 1A

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Positive Sequencing Control 1A


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Negative Sequencing Control 1B

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Negative Sequencing Control 1B


text: NORTHUMBRIA UNIVERSITY, NEWCASTLE

text: public

text: Positive Sequencing Control 1B

text: United Kingdom

text: dust metagenome

text: dust metagenome

text: Positive Sequencing Control 1B


text: CEDIMOL

text: public

text: ena-SAMPLE-TAB-10-12-2023-23:17:33:016-73935

text: DNA

text: Colombia

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

characteristic: isolate

text: Human

text: not provided

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: not provided

characteristic: serovar

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CEDIMOL

text: public

text: ena-SAMPLE-TAB-10-12-2023-23:17:33:017-73936

text: DNA

text: Colombia

text: not provided

text: Homo sapiens

text: not provided

text: Human

text: not provided

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: not provided


text: CEDIMOL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-10-12-2023-23:17:33:017-73937

text: DNA

text: Colombia

text: not provided

text: Homo sapiens

text: not provided

text: Human

text: not provided

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: not provided


text: CEDIMOL

text: public

characteristic: SRA accession

text: ena-SAMPLE-TAB-10-12-2023-23:17:33:017-73938

characteristic: Submitter Id

characteristic: collection date

text: DNA

characteristic: description

text: Colombia

text: not provided

text: Homo sapiens

text: not provided

text: Human

text: not provided

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: not provided


text: CEDIMOL

text: public

text: ena-SAMPLE-TAB-10-12-2023-23:17:33:017-73939

text: DNA

text: Colombia

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: host health state

text: Homo sapiens

text: not provided

text: Human

text: not provided

text: Mycobacterium tuberculosis

characteristic: organism

text: Mycobacterium tuberculosis

text: not provided

characteristic: title


text: CEDIMOL

text: public

text: ena-SAMPLE-TAB-10-12-2023-23:17:33:017-73941

text: DNA

text: Colombia

text: not provided

text: Homo sapiens

characteristic: host scientific name

text: not provided

characteristic: isolate

text: Human

text: not provided

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: not provided

characteristic: serovar

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CEDIMOL

text: public

text: ena-SAMPLE-TAB-10-12-2023-23:17:33:017-73943

text: DNA

text: Colombia

text: not provided

text: Homo sapiens

text: not provided

text: Human

text: not provided

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: not provided


text: CEDIMOL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-10-12-2023-23:17:33:017-73944

text: DNA

text: Colombia

text: not provided

text: Homo sapiens

text: not provided

text: Human

text: not provided

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: not provided


text: CEDIMOL

text: public

characteristic: SRA accession

text: ena-SAMPLE-TAB-10-12-2023-23:17:33:017-73945

characteristic: Submitter Id

characteristic: collection date

text: DNA

characteristic: description

text: Colombia

text: not provided

text: Homo sapiens

text: not provided

text: Human

text: not provided

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: not provided


text: CEDIMOL

text: public

text: ena-SAMPLE-TAB-10-12-2023-23:17:33:017-73946

text: DNA

text: Colombia

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: host health state

text: Homo sapiens

text: not provided

text: Human

text: not provided

text: Mycobacterium tuberculosis

characteristic: organism

text: Mycobacterium tuberculosis

text: not provided

characteristic: title


text: CEDIMOL

text: public

text: ena-SAMPLE-TAB-10-12-2023-23:17:33:017-73947

text: DNA

text: Colombia

text: not provided

text: Homo sapiens

characteristic: host scientific name

text: not provided

characteristic: isolate

text: Human

text: not provided

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: not provided

characteristic: serovar

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CEDIMOL

text: public

text: ena-SAMPLE-TAB-10-12-2023-23:17:33:017-73948

text: DNA

text: Colombia

text: not provided

text: Homo sapiens

text: not provided

text: Human

text: not provided

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: not provided


text: CEDIMOL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-10-12-2023-23:17:33:018-73950

text: DNA

text: Colombia

text: not provided

text: Homo sapiens

text: not provided

text: Human

text: not provided

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: not provided


text: CEDIMOL

text: public

characteristic: SRA accession

text: ena-SAMPLE-TAB-10-12-2023-23:17:33:018-73951

characteristic: Submitter Id

characteristic: collection date

text: DNA

characteristic: description

text: Colombia

text: not provided

text: Homo sapiens

text: not provided

text: Human

text: not provided

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: not provided


text: CEDIMOL

text: public

text: ena-SAMPLE-TAB-10-12-2023-23:17:33:018-73952

text: DNA

text: Colombia

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: host health state

text: Homo sapiens

text: not provided

text: Human

text: not provided

text: Mycobacterium tuberculosis

characteristic: organism

text: Mycobacterium tuberculosis

text: not provided

characteristic: title


Kullaberg

text: STOCKHOLM UNIVERSITY

text: public

text: Kullaberg

tag: Namespace:STOCKHOLM UNIVERSITY

text: Pool of 24 adult female butterflies

text: Adult

text: Sweden

text: Kullaberg

characteristic: geographic location (region and locality)

text: Pieris napi napi

text: Pieris napi napi

text: F

characteristic: sex

text: Pieris_napi.SWE.14.Skane_Kullaberg.Field_females.Thorax.24


Lulea

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: STOCKHOLM UNIVERSITY

text: public

text: Lulea

text: Pool of 30 adult female butterflies

text: Adult

text: Sweden

text: Lulea

text: Pieris napi napi

text: Pieris napi napi

text: F

text: Pieris_napi.SWE.15.Lulea_Avan.Field.Thorax.30


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Institute of microbiology CAS

text: public

tag: Namespace:Institute of microbiology CAS

text: stool samples

text: Czech Republic

text: metagenome

text: metagenome

text: gut microbiota


text: ZTRI

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Pacbio Hifi genome sequencing

text: adult

text: China

text: Helicoverpa assulta

text: Helicoverpa assulta

text: female

text: whole body

text: illumina Hic genome sequencing of F1


text: ETH ZURICH

characteristic: INSDC last update

text: public

characteristic: INSDC status

tag: Namespace:ETH ZURICH

text: blood

text: European bison

characteristic: common name

text: male

text: Switzerland

text: Bison bonasus

text: Bison bonasus

text: Urano


text: UNIBE-IG

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: goat

text: hair sample

text: Switzerland

text: Capra hircus

text: Capra hircus

text: Chamois colored


text: UNIBE-IG

text: public

text: goat

text: hair sample

characteristic: description

text: Switzerland

characteristic: geographic location (country and/or sea)

text: Capra hircus

text: Capra hircus

text: Peacock


text: UNIBE-IG

text: public

text: goat

text: hair sample

text: Switzerland

text: Capra hircus

characteristic: organism

text: Capra hircus

text: Saanen

characteristic: title


text: UNIBE-IG

text: public

text: goat

text: blood sample

text: Switzerland

text: Capra hircus

text: Capra hircus

text: Boer goat


text: kpeters

text: public

text: Kristian Peters

text: iDiv

characteristic: collecting institution

text: Sweden

text: Sweden

characteristic: geographic location (region and locality)

text: biological soil crust

characteristic: habitat

characteristic: latitude

text: gametophyte

characteristic: lifestage

characteristic: longitude

text: Mannia gracilis

text: thallus

characteristic: organism part

characteristic: project name

text: Mannia gracilis

text: not provided

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: kpeters

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Kristian Peters

text: iDiv

text: Sweden

text: Sweden

text: biological soil crust

text: gametophyte

text: Clevea hyalina

text: thallus

text: Clevea hyalina

text: not provided


text: kpeters

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Kristian Peters

text: iDiv

characteristic: collecting institution

characteristic: collection date

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

text: Sweden

text: biological soil crust

text: gametophyte

text: Mannia fragrans

characteristic: organism

text: thallus

text: Mannia fragrans

text: not provided

characteristic: title


text: kpeters

text: public

text: Kristian Peters

text: iDiv

text: Sweden

text: Sweden

characteristic: geographic location (region and locality)

text: biological soil crust

characteristic: habitat

characteristic: latitude

text: gametophyte

characteristic: lifestage

characteristic: longitude

text: Reboulia hemisphaerica

text: thallus

characteristic: organism part

characteristic: project name

text: Reboulia hemisphaerica

text: not provided

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: kpeters

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Kristian Peters

text: iDiv

text: Sweden

text: Sweden

text: biological soil crust

text: gametophyte

text: Riccia beyrichiana

text: thallus

text: Riccia beyrichiana

text: not provided


text: kpeters

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Kristian Peters

text: iDiv

characteristic: collecting institution

characteristic: collection date

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

text: Sweden

text: biological soil crust

text: gametophyte

text: Riccia bifurca

characteristic: organism

text: thallus

text: Riccia bifurca

text: not provided

characteristic: title


text: kpeters

text: public

text: Kristian Peters

text: iDiv

text: Sweden

text: Sweden

characteristic: geographic location (region and locality)

text: biological soil crust

characteristic: habitat

characteristic: latitude

text: gametophyte

characteristic: lifestage

characteristic: longitude

text: Riccia canaliculata

text: thallus

characteristic: organism part

characteristic: project name

text: Riccia canaliculata

text: not provided

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: kpeters

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Kristian Peters

text: iDiv

text: Sweden

text: Sweden

text: biological soil crust

text: gametophyte

text: Riccia cavernosa

text: thallus

text: Riccia cavernosa

text: not provided


text: kpeters

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Kristian Peters

text: iDiv

characteristic: collecting institution

characteristic: collection date

characteristic: description

text: Germany

characteristic: geographic location (country and/or sea)

text: Germany

text: biological soil crust

text: gametophyte

text: Riccia ciliifera

characteristic: organism

text: thallus

text: Riccia ciliifera

text: not provided

characteristic: title


text: kpeters

text: public

text: Kristian Peters

text: iDiv

text: Sweden

text: Sweden

characteristic: geographic location (region and locality)

text: biological soil crust

characteristic: habitat

characteristic: latitude

text: gametophyte

characteristic: lifestage

characteristic: longitude

text: Riccia ciliifera

text: thallus

characteristic: organism part

characteristic: project name

text: Riccia ciliifera

text: not provided

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: kpeters

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Kristian Peters

text: iDiv

text: Sweden

text: Sweden

text: biological soil crust

text: gametophyte

text: Riccia gothica

text: thallus

text: Riccia gothica

text: not provided


text: kpeters

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Kristian Peters

text: iDiv

characteristic: collecting institution

characteristic: collection date

characteristic: description

text: Sweden

characteristic: geographic location (country and/or sea)

text: Sweden

text: biological soil crust

text: gametophyte

text: Riccia huebeneriana

characteristic: organism

text: thallus

text: Riccia huebeneriana

text: not provided

characteristic: title


text: kpeters

text: public

text: Kristian Peters

text: iDiv

text: Sweden

text: Sweden

characteristic: geographic location (region and locality)

text: biological soil crust

characteristic: habitat

characteristic: latitude

text: gametophyte

characteristic: lifestage

characteristic: longitude

text: Riccia sorocarpa

text: thallus

characteristic: organism part

characteristic: project name

text: Riccia sorocarpa

text: not provided

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: kpeters

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Kristian Peters

text: iDiv

text: Sweden

text: Sweden

text: biological soil crust

text: gametophyte

text: Riccia subbifurca

text: thallus

text: Riccia subbifurca

text: not provided


text: FISABIONGS

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: FISABIONGS

characteristic: broker name

characteristic: collection date

text: feces

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

text: pig gut metagenome

characteristic: organism

text: pig gut metagenome

characteristic: title


characteristic: ENA first public

text: Universidad de León

text: public

tag: Namespace:Universidad de León

text: ArrayExpress

text: milk somatic cell

text: not collected

text: sheep

characteristic: common name

text: Protocols: Milk samples for RNA-Seq were collected at the period when FE was evaluated, between Days 40-50 at first lactation.Briefly, for each animal, 50 ml of milk was collected one hour after morning milking and ten minutes after the injection of 5 IU of Oxitocine Facilpart (Syva, León, Spain) to maximize the number of exfoliated milk epithelial cells . To avoid RNA degradation during sampling collection, the udder was disinfected with povidone-iodine, nipples were cleaned with RNAseZap (Ambion, Austin, TX, USA), and sterile gauze was used to cover the collection container (i.e., 50 ml RNAse-free tube). Forty Spanish Assaf female lambs (86.7 days of age; SD=2.5) were bought from a single flock and were transported to the facilities of the Instituto de Ganadería de Montaña (IGM; CSIC- Universidad de León). The animals were managed under standard commercial conditions; ewe lambs, i.e., female lambs, were fed a standard diet for replacement ewe lambs, consisting of 16% crude protein. At three months of age, they were divided into two groups: 20 nutritional protein-restricted ewes (NPR) and 20 control ewes. The protein restriction period was applied in NPR ewes during the mammary gland allometric growth period (64 days, between 3.5 and 5.8 months of age). Thus, the control ewes received the standard diet mentioned above for 64 d, and during the same period, the NPR ewes received the same diet without soybean meal (42.3% reduction in protein content). Both groups had ad libitum access to barley straw. Estrus was synchronized to avoid differences due to the lactation stage, the ewes were artificially inseminated at approximately 10 months of age, and lambing was concentrated within a timeframe of a few days. During the first lactation, milk production was recorded daily, and milk composition (protein and fat content) was analyzed regularly for each ewe from weeks 1 to 12 after lambing. Thus, for each animal, a total of 27 records, including milk yield (MY) and milk protein and fat percentages (PP and FP, respectively), were available. On Day 33 of lactation, the ewes were housed in individual pens. To estimate FE, individual feed intake and milk production were measured over 28 days after the ewes adapting not only to the diet but also to the individual stalls. During lactation, the ewes were fed a total mixed ration (TMR) formulated from alfalfa hay (particle size > 4 cm) and concentrate (50:50 forage:concentrate ratio), including sugar beet molasses, to hinder the selection of dietary components. The ingredients and chemical composition of the TMR are reported in Table 1. Clean drinking water was made consistently available. Feed intake was calculated by weighing the amounts of dry matter (DM) offered and refused by each animal. The ewes were milked twice daily at approximately 08:30 and 18:30 h in a single-side milking parlor with 10 stalls (DeLaval, Madrid, Spain). The total milk produced by each animal at both milkings was collected and weighed to calculate the milk yield. Composite samples of the milk produced by each ewe were prepared daily according to individual yields in both milkings. One aliquot (40 mL) of that composite milk was collected on Days 4, 5, 9, 13, 14, 15, 23 and 28 of the trial, preserved with bronopol (D&F Control Systems Inc., San Ramon, CA, USA) and stored at 4 °C until analyzed for fat, protein, lactose, and total solids concentrations and for somatic cell count. FE was calculated using two indices: RFI and FCR. The FCR was calculated as the ratio between DMI and the energy-corrected milk yield (ECM): FCR = DMI/ECM, where DMI represents the mean dry matter intake during the experimental period (kg/d), and ECM (L/d) was calculated using the formula proposed by INRA [25] for sheep: ECM = L/d milk yield × [(0.0071 × g/L milk fat) + (0.0043 × g/L milk protein) + 0.2224 The RFI as the residuals of the following regression model, using the glm procedure of the R software (v4.2.1): DMI = µ + a × ECM + b × MBW + c × BW×BWC + RFI, where DMI represents the mean dry matter intake during the experimental period (kg/d); µ is the intercept; ECM is the energy-corrected milk yield (kg/d); MBW is the mean metabolic body weight (BW) (BW0.75; kg); BW×BWC is the interaction between the body weight (BW in kg) and the BW change per day (kg/d); RFI is the residual term; and a, b, and c are the regression coefficients. High-FE and Low-FE (phenotype column) are those animals that could be classified as high-FE or low-FE based on FCR and RFI indices After milk collection, the milk somatic cells (MSCs) were pelleted by centrifugation at 650×g for 10 minutes at 4 °C and in the presence of a final concentration of 0.5 mM EDTA to eliminate casein and fat globules. Then, the MSC pellet was washed twice with 10 and 2 mL of PBS (pH 7.2 and 0.5 mM EDTA), followed by centrifugation at 650×g for 10 min at 4 °C. The last pellet was kept in RNAlater (Sigma‒Aldrich, Madrid, Spain) and stored at −80 °C until RNA extraction using 500 μL of TRIzol according to the manufacturer's instructions (Invitrogen, Carlsbad, CA, USA). The RNA quality was evaluated using an Agilent 2,100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Then, 28 samples with enough RNA quality (RIN > 6.4) were sent to be sequenced at Novogene (Cambridge, UK). The UltraTM RNA Library Prep Kit (NEBNext®) was used to construct the libraries.

text: primiparous

characteristic: developmental stage

text: control

characteristic: diet

text: not collected

characteristic: individual

text: not applicable

characteristic: isolate

text: Ovis aries

text: Ovis aries

text: mammary gland

characteristic: organism part

characteristic: phenotype

text: Ovis aries

text: female

characteristic: sex

text: Assaf

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: External Id

text: Universidad de León

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: milk somatic cell

text: not collected

text: sheep

text: primiparous

text: protein restriction

text: not collected

text: not applicable

text: Ovis aries

text: Ovis aries

text: mammary gland

text: Ovis aries

text: female

text: Assaf


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: LNU

characteristic: INSDC first public

text: public

characteristic: collection date

text: WH-5701 sp015841355 KAC 105 full genome

characteristic: description

text: Baltic Sea

characteristic: geographic location (country and/or sea)

text: Synechococcus sp. CBW1107

characteristic: organism

text: Synechococcus sp. CBW1107

characteristic: title


text: LNU

text: public

text: Cyanobium_A KAC 108 full genome

text: Baltic Sea

text: Cyanobium usitatum str. Tous

text: Cyanobium usitatum str. Tous


text: LNU

text: public

text: CBW1002 sp015840915 KAC 102 full genome

text: Baltic Sea

text: Synechococcus sp. CBW1107

text: Synechococcus sp. CBW1107


text: LNU

text: public

text: WH-5701 sp000153045 KAC 106 full genome

text: Baltic Sea

text: Synechococcus sp. CBW1107

text: Synechococcus sp. CBW1107


text: LNU

text: public

text: Vulcanococcus sp018502385 KAC 114 full genome

text: Baltic Sea

text: Synechococcus sp. CBW1107

text: Synechococcus sp. CBW1107


text: NMFRI

text: public

text: freshwater biome

characteristic: broad-scale environmental context

text: DADA2 version 1.18.0; function: removeBimeraDenovo; method=pooled

characteristic: chimera check software

unit: m

characteristic: depth

text: Treatment: Control; Light condition: Dark; Replicate: A; Time: 0h

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1348278.75 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 156459.6 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

text: intital denaturation:98degC_3min;denaturation:98degC_10s; annealing:52degC_30s;elongation:72degC_30s;number of cycles:30; final elongation:72degC_5min

characteristic: pcr conditions

text: pufM_uniF: GGNAAYYTNTWYTAYAAY; pufM_uniR: YCCATNGTCCANCKCCARAACCNTTYCA

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

unit: days

characteristic: sample storage duration

text: Centre Algatech, freezere on the coridor

characteristic: sample storage location

unit: mL

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Control; Light condition: Dark; Replicate: B; Time: 0h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1536939 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 193203.9 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Control; Light condition: Dark; Replicate: C; Time: 0h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1550372.4 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 162386.1 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Control; Light condition: IR; Replicate: A; Time: 0h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1077545.45454545 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 137494.8 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Control; Light condition: IR; Replicate: B; Time: 0h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1248516 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 141050.7 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Control; Light condition: IR; Replicate: C; Time: 0h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1300274.1 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 169497.9 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Dilution; Light condition: Dark; Replicate: A; Time: 0h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

text: DADA2 version 1.18.0; function: removeBimeraDenovo; method=pooled

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Dilution; Light condition: Dark; Replicate: B; Time: 0h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Dilution; Light condition: Dark; Replicate: C; Time: 0h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Dilution; Light condition: IR; Replicate: A; Time: 0h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1348278.75 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 156459.6 cells per ml; epifluorescent microscopy

text: aerobic

text: intital denaturation:98degC_3min;denaturation:98degC_10s; annealing:52degC_30s;elongation:72degC_30s;number of cycles:30; final elongation:72degC_5min

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Dilution; Light condition: IR; Replicate: B; Time: 0h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

text: pufM_uniF: GGNAAYYTNTWYTAYAAY; pufM_uniR: YCCATNGTCCANCKCCARAACCNTTYCA

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

text: Centre Algatech, freezere on the coridor

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Dilution; Light condition: IR; Replicate: C; Time: 0h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1550372.4 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 162386.1 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Lignin; Light condition: Dark; Replicate: A; Time: 0h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1536939 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 193203.9 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Lignin; Light condition: Dark; Replicate: B; Time: 0h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1077545.45454545 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 137494.8 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Lignin; Light condition: Dark; Replicate: C; Time: 0h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Lignin; Light condition: IR; Replicate: A; Time: 0h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1300274.1 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 169497.9 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: DADA2 version 1.18.0; function: removeBimeraDenovo; method=pooled

text: Treatment: Lignin; Light condition: IR; Replicate: B; Time: 0h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Lignin; Light condition: IR; Replicate: C; Time: 0h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1608057 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 196759.8 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Control; Light condition: Dark; Replicate: A; Time: 14.25h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1333462.5 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 292769.1 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

text: intital denaturation:98degC_3min;denaturation:98degC_10s; annealing:52degC_30s;elongation:72degC_30s;number of cycles:30; final elongation:72degC_5min

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Control; Light condition: Dark; Replicate: B; Time: 14.25h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1218488.4 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 271433.7 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM_uniF: GGNAAYYTNTWYTAYAAY; pufM_uniR: YCCATNGTCCANCKCCARAACCNTTYCA

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: Centre Algatech, freezere on the coridor

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Control; Light condition: Dark; Replicate: C; Time: 14.25h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1206372 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 297510.3 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Control; Light condition: IR; Replicate: A; Time: 14.25h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1179373.5 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 216909.9 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Control; Light condition: IR; Replicate: B; Time: 14.25h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1227632.14285714 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 200315.7 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

text: DADA2 version 1.18.0; function: removeBimeraDenovo; method=pooled

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Control; Light condition: IR; Replicate: C; Time: 14.25h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1191765.27272727 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 201501 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Dilution; Light condition: Dark; Replicate: A; Time: 20h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 713814 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 80600.4 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Dilution; Light condition: Dark; Replicate: B; Time: 20h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 665249.625 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 94824 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Dilution; Light condition: Dark; Replicate: C; Time: 20h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 894901.5 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 129197.7 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

text: intital denaturation:98degC_3min;denaturation:98degC_10s; annealing:52degC_30s;elongation:72degC_30s;number of cycles:30; final elongation:72degC_5min

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Dilution; Light condition: IR; Replicate: A; Time: 20h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 609582.857142857 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 158830.2 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM_uniF: GGNAAYYTNTWYTAYAAY; pufM_uniR: YCCATNGTCCANCKCCARAACCNTTYCA

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: Centre Algatech, freezere on the coridor

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Dilution; Light condition: IR; Replicate: B; Time: 20h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 322401.6 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 82971 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Dilution; Light condition: IR; Replicate: C; Time: 20h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 485973 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 120900.6 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: DADA2 version 1.18.0; function: removeBimeraDenovo; method=pooled

text: Treatment: Lignin; Light condition: Dark; Replicate: A; Time: 19.6h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 879492.6 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 98379.9 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Lignin; Light condition: Dark; Replicate: B; Time: 19.6h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1302348.375 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 126827.1 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Lignin; Light condition: Dark; Replicate: C; Time: 19.6h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 892926 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 113788.8 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Lignin; Light condition: IR; Replicate: A; Time: 19.6h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 875146.5 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 118530 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Lignin; Light condition: IR; Replicate: B; Time: 19.6h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 860958.818181818 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 135124.2 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

text: intital denaturation:98degC_3min;denaturation:98degC_10s; annealing:52degC_30s;elongation:72degC_30s;number of cycles:30; final elongation:72degC_5min

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Lignin; Light condition: IR; Replicate: C; Time: 19.6h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 771432.75 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 110232.9 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM_uniF: GGNAAYYTNTWYTAYAAY; pufM_uniR: YCCATNGTCCANCKCCARAACCNTTYCA

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: Centre Algatech, freezere on the coridor

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Control; Light condition: Dark; Replicate: A; Time: 26.25h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1169496 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 177795 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

text: DADA2 version 1.18.0; function: removeBimeraDenovo; method=pooled

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Control; Light condition: Dark; Replicate: B; Time: 26.25h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1224414.9 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 241801.2 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Control; Light condition: Dark; Replicate: C; Time: 26.25h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1518877.28571429 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 254839.5 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Control; Light condition: IR; Replicate: A; Time: 26.25h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1145790 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 278545.5 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Control; Light condition: IR; Replicate: B; Time: 26.25h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1539196.71428571 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 384037.2 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Control; Light condition: IR; Replicate: C; Time: 26.25h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1398654 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 359145.9 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: Treatment: Dilution; Light condition: Dark; Replicate: A; Time: 32h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1096402.5 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 207427.5 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

text: intital denaturation:98degC_3min;denaturation:98degC_10s; annealing:52degC_30s;elongation:72degC_30s;number of cycles:30; final elongation:72degC_5min

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Dilution; Light condition: Dark; Replicate: B; Time: 32h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1255232.7 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 257210.1 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM_uniF: GGNAAYYTNTWYTAYAAY; pufM_uniR: YCCATNGTCCANCKCCARAACCNTTYCA

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: Centre Algatech, freezere on the coridor

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

text: public

text: freshwater biome

text: DADA2 version 1.18.0; function: removeBimeraDenovo; method=pooled

text: Treatment: Dilution; Light condition: Dark; Replicate: C; Time: 32h

text: coniferous forest

text: Czech Republic

characteristic: geographic location (longitude)

text: South Bohemia, Lake Cep

characteristic: geographic location (region and locality)

text: freshwater lake

characteristic: local environmental context

characteristic: nucleic acid extraction

text: freshwater metagenome

text: total prokaryotes; 1384430.4 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 263136.6 cells per ml; epifluorescent microscopy

characteristic: organism count

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr conditions

characteristic: pcr primers

text: pufM study

characteristic: project name

text: Ruttner Water Sampler (model 11.003KC)

characteristic: sample collection device

text: less than 6 months

characteristic: sample storage duration

characteristic: sample storage location

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: pufM

characteristic: target gene

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NMFRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: chimera check software

characteristic: collection date

characteristic: depth

text: Treatment: Dilution; Light condition: IR; Replicate: A; Time: 32h

characteristic: description

text: coniferous forest

characteristic: environmental medium

text: Czech Republic

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Bohemia, Lake Cep

text: freshwater lake

text: freshwater metagenome

characteristic: organism

text: total prokaryotes; 1181913.42857143 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 259580.7 cells per ml; epifluorescent microscopy

text: aerobic

text: pufM study

text: Ruttner Water Sampler (model 11.003KC)

text: less than 6 months

text: freshwater metagenome

text: Illumina MiSeq

text: pufM

characteristic: title