status: PUBLIC

characteristic: ENA first public

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: External Id

text: EMBL

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: ESC 129-B13

text: mouse embryonic stem cell

text: house mouse

characteristic: common name

text: Protocols: Mouse embryonic stem cells (129XC57BL/6J) were cultured in ESC medium containing Knockout-DMEM (Thermo Fisher Scientific) supplemented with 15% EmbryoMax fetal bovine serum (ES-FBS Merck-Millipore) and 20 ng/ml leukemia inhibitory factor (LIF, produced by Protein Expression and Purification Facility at EMBL Heidelberg), 1% non-essential amino acids (Thermo Fisher Scientific), 1% Glutamax (Thermo Fisher Scientific), 1% Pen/Strep (Thermo Fisher Scientific), 1% of 55 mM beta-Mercaptoethanol solution (Merck Millipore). Upon thawing, cells were cultured on a layer of mouse embryonic fibroblasts (MEFs) and afterwards passaged on 0.1% gelatine-coated culture dishes, without feeder cells. Cells were maintained at 37°C with 5% CO2 and routinely tested for mycoplasma. The medium was changed daily, and cells were passed every two days. DNA for native ChIP was digested by MNase treatment to obtain mainly mono-nucleosomes using a modified protocol from Barski et al., 2007 (PMID: 17512414). For each IP, 20x106 cells were resuspended in digestion buffer (50 mM Tris-HCl pH 7.6; 1 mM CaCl2; 0.2% Triton-X), treated with 100 U MNase (Worthington) and incubated at 37°C for 5 minutes while shaking at 500 rpm. Samples were quickly moved to ice and MNase was quenched by addition of 5 mM EDTA (final). Lysates were sonicated for 3 cycles using Bioruptor Pico (Diagenode) and dialyzed against RIPA buffer for 3 hours at 4°C. Insoluble materials was pelleted at 10,000 rpm for 10 minutes at 4°C and supernatant was used as input for ChIP. To check the fragmentation pattern, input DNA was analyzed by agarose gel electrophoresis. A 5% fraction of input was set aside for sequencing. For native ChIP of histone modifications, Protein-G-Dynabeads (Invitrogen) were pre-coated with antibodies for 4 hours at 4°C. After overnight incubation of the pre-coated beads with chromatin lysates, beads were washed (3X with RIPA; 2X with RIPA + 300 mM NaCl; 2X with LiCl buffer-250 mM LiCl, 0.5% NP-40, 0.5% NaDoc) and finally rinsed with TE + 50 mM NaCl Buffer. Samples were eluted from ProteinG Dynabeads using SDS elution buffer (50 mM Tris-HCl ph 8.0; 10 mM EDTA; 1% SDS) at 65°C for 30 minutes with shaking at 1500 rpm. Proteinase K was added (0.2 µg/ml final) to the eluted samples and digestion was carried out for 2 hours at 55°C followed by QIAquick PCR purification (Qiagen). Sequencing libraries were prepared using the NEBNext Ultra II DNA Library Preparation Kit (New England Biolabs) and, after Qubit quantification, the quality of individual libraries was assessed on a Bioanalyzer using the Agilent High Sensitivity DNA Kit.

characteristic: description

text: targeted mutation

characteristic: genetic modification

text: H3f3a knockout; H3f3b K9A

characteristic: genotype

text: not applicable

characteristic: isolate

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Mus musculus

text: frozen specimen

characteristic: specimen with known storage state

characteristic: title


characteristic: ENA first public

text: EMBL

text: public

text: ArrayExpress

text: ESC 129-B13

text: mouse embryonic stem cell

text: house mouse

text: Protocols: Mouse embryonic stem cells (129XC57BL/6J) were cultured in ESC medium containing Knockout-DMEM (Thermo Fisher Scientific) supplemented with 15% EmbryoMax fetal bovine serum (ES-FBS Merck-Millipore) and 20 ng/ml leukemia inhibitory factor (LIF, produced by Protein Expression and Purification Facility at EMBL Heidelberg), 1% non-essential amino acids (Thermo Fisher Scientific), 1% Glutamax (Thermo Fisher Scientific), 1% Pen/Strep (Thermo Fisher Scientific), 1% of 55 mM beta-Mercaptoethanol solution (Merck Millipore). Upon thawing, cells were cultured on a layer of mouse embryonic fibroblasts (MEFs) and afterwards passaged on 0.1% gelatine-coated culture dishes, without feeder cells. Cells were maintained at 37°C with 5% CO2 and routinely tested for mycoplasma. The medium was changed daily, and cells were passed every two days. The protocol was adapted from Groh et al., 2021 (PMID: 34650047) and Navarro et al., 2020 (PMID: 33037201). Cells were harvested and crosslinked in 3 ml of pre-tempered (25°C) ES medium supplemented with 1% formaldehyde (106 cells/3 ml) for 10 minutes at RT, with rotation. Formaldehyde was then quenched with glycine (125 mM final) and incubated 5 minutes at RT, with rotation. Cells were washed twice with ice-cold PBS containing 10%FBS (centrifugation at 1100 rpm for 4 minutes, at 4°C). Pellets can be stored at -80°C for several months. To prepare lysates, fixed cells were resuspended in 300 µl of Sonication Buffer 1 (50 mM Tris-HCl pH 8.0; 0.5% SDS) and incubated for 10 minutes on ice. Sonication was performed with Bioruptor Pico (Diagenode) for 20 cycles (30’’ON/30’’OFF). Sonicated lysates were then diluted 1:6 with lysis buffer (10 mM Tris-HCl ph 8.0; 100 mM NaCl; 1% Triton-X; 1 mM EDTA; 0.5 mM EGTA; 0.1% NaDoc; 0.5% N-laurolsarcosine) and the soluble fraction was collected after centrifugation at full speed for 10 minutes at 4°C. A 2.5% fraction of the supernatant was set aside for input and the fragmentation pattern (~150-500 bp) was checked by agarose gel electrophoresis. Lysates can be aliquoted and stored at -80°C with 10% glycerol (final). For each IP, 30 µl Protein G Dynabeads (Invitrogen) were washed twice with 1 ml PBS-T (PBS + 0.01% Tween-20) and incubated with the desired antibody for at least 1 hour at RT (or > 1 hour at 4°C). Coated beads were then washed (1X with PBS-T; 2X with lysis buffer), resuspended in 30 µl of lysis buffer/IP and added to the desired amount of chromatin (generally 15-25 µg of chromatin were used, corresponding to 2-4x106 cells). After overnight incubation at 4°C with rotation, beads-immunocomplexes were washed twice-each time for 5 minutes-with the following buffers: RIPA; RIPA + 360 mM NaCl; LiCl buffer (10 mM Tris-HCl ph 8.0; 250 mM LiCl, 0.5% NP-40, 0.5% NaDoc; 1 mM EDTA) and finally quickly rinsed with TE buffer and eluted in ChIP SDS elution buffer (10 mM Tris-HCl ph 8.0; 300 mM NaCl; 5 mM EDTA; 0.5% SDS). RNA/protein digestion was performed on beads by adding 2 µl  RNaseA (10 mg/ml stock) incubated 30 minutes at 37°C, followed by addition of 1.5 µl Proteinase K (20 mg/ml stock) incubated 1h @ 55°C. Reverse cross-link was performed with overnight incubation at 65°C. DNA was purified with 1.4X SPRI-select beads or QIAquick PCR Purification kit (Qiagen). Sequencing libraries were prepared using the NEBNext Ultra II DNA Library Preparation Kit (New England Biolabs) and, after Qubit quantification, the quality of individual libraries was assessed on a Bioanalyzer using the Agilent High Sensitivity DNA Kit.

text: targeted mutation

text: H3f3a knockout; H3f3b K9A

text: not applicable

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: frozen specimen


characteristic: ENA-CHECKLIST

text: EMBL

text: public

text: ArrayExpress

text: ESC 129-B13

text: mouse embryonic stem cell

text: house mouse

text: Protocols: Mouse embryonic stem cells (129XC57BL/6J) were cultured in ESC medium containing Knockout-DMEM (Thermo Fisher Scientific) supplemented with 15% EmbryoMax fetal bovine serum (ES-FBS Merck-Millipore) and 20 ng/ml leukemia inhibitory factor (LIF, produced by Protein Expression and Purification Facility at EMBL Heidelberg), 1% non-essential amino acids (Thermo Fisher Scientific), 1% Glutamax (Thermo Fisher Scientific), 1% Pen/Strep (Thermo Fisher Scientific), 1% of 55 mM beta-Mercaptoethanol solution (Merck Millipore). Upon thawing, cells were cultured on a layer of mouse embryonic fibroblasts (MEFs) and afterwards passaged on 0.1% gelatine-coated culture dishes, without feeder cells. Cells were maintained at 37°C with 5% CO2 and routinely tested for mycoplasma. The medium was changed daily, and cells were passed every two days. The protocol was adapted from Groh et al., 2021 (PMID: 34650047) and Navarro et al., 2020 (PMID: 33037201). Cells were harvested and crosslinked in 3 ml of pre-tempered (25°C) ES medium supplemented with 1% formaldehyde (106 cells/3 ml) for 10 minutes at RT, with rotation. Formaldehyde was then quenched with glycine (125 mM final) and incubated 5 minutes at RT, with rotation. Cells were washed twice with ice-cold PBS containing 10%FBS (centrifugation at 1100 rpm for 4 minutes, at 4°C). Pellets can be stored at -80°C for several months. To prepare lysates, fixed cells were resuspended in 300 µl of Sonication Buffer 1 (50 mM Tris-HCl pH 8.0; 0.5% SDS) and incubated for 10 minutes on ice. Sonication was performed with Bioruptor Pico (Diagenode) for 20 cycles (30’’ON/30’’OFF). Sonicated lysates were then diluted 1:6 with lysis buffer (10 mM Tris-HCl ph 8.0; 100 mM NaCl; 1% Triton-X; 1 mM EDTA; 0.5 mM EGTA; 0.1% NaDoc; 0.5% N-laurolsarcosine) and the soluble fraction was collected after centrifugation at full speed for 10 minutes at 4°C. A 2.5% fraction of the supernatant was set aside for input and the fragmentation pattern (~150-500 bp) was checked by agarose gel electrophoresis. Lysates can be aliquoted and stored at -80°C with 10% glycerol (final). For each IP, 30 µl Protein G Dynabeads (Invitrogen) were washed twice with 1 ml PBS-T (PBS + 0.01% Tween-20) and incubated with the desired antibody for at least 1 hour at RT (or > 1 hour at 4°C). Coated beads were then washed (1X with PBS-T; 2X with lysis buffer), resuspended in 30 µl of lysis buffer/IP and added to the desired amount of chromatin (generally 15-25 µg of chromatin were used, corresponding to 2-4x106 cells). For ChIP-Rx, spike-in chromatin was prepared from Drosophila Schneider 2 (S2) cells following the same protocol and aliquots were stored at -80°C with 10% glycerol. Before immunoprecipitation, 3% of exogenous chromatin was added to each reaction. After overnight incubation at 4°C with rotation, beads-immunocomplexes were washed twice-each time for 5 minutes-with the following buffers: RIPA; RIPA + 360 mM NaCl; LiCl buffer (10 mM Tris-HCl ph 8.0; 250 mM LiCl, 0.5% NP-40, 0.5% NaDoc; 1 mM EDTA) and finally quickly rinsed with TE buffer and eluted in ChIP SDS elution buffer (10 mM Tris-HCl ph 8.0; 300 mM NaCl; 5 mM EDTA; 0.5% SDS). RNA/protein digestion was performed on beads by adding 2 µl  RNaseA (10 mg/ml stock) incubated 30 minutes at 37°C, followed by addition of 1.5 µl Proteinase K (20 mg/ml stock) incubated 1h @ 55°C. Reverse cross-link was performed with overnight incubation at 65°C. DNA was purified with 1.4X SPRI-select beads or QIAquick PCR Purification kit (Qiagen). Sequencing libraries were prepared using the NEBNext Ultra II DNA Library Preparation Kit (New England Biolabs) and, after Qubit quantification, the quality of individual libraries was assessed on a Bioanalyzer using the Agilent High Sensitivity DNA Kit.

text: targeted mutation

text: H3f3a knockout; H3f3b K9A

text: not applicable

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: frozen specimen


characteristic: External Id

text: EMBL

text: public

text: ArrayExpress

text: ESC 129-B13

text: mouse embryonic stem cell

text: house mouse

text: targeted mutation

text: H3f3a knockout; H3f3b K27A

text: not applicable

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: frozen specimen


text: EMBL

characteristic: INSDC center name

text: public

text: ArrayExpress

text: ESC 129-B13

text: mouse embryonic stem cell

text: house mouse

text: targeted mutation

text: H3f3a knockout; H3f3b K27A

text: not applicable

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: frozen specimen


text: EMBL

characteristic: INSDC last update

text: public

text: ArrayExpress

text: ESC 129-B13

text: mouse embryonic stem cell

text: house mouse

text: targeted mutation

text: H3f3a knockout; H3f3b K27A

text: not applicable

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: frozen specimen


text: EMBL

text: public

characteristic: INSDC status

text: ArrayExpress

text: ESC 129-B13

text: mouse embryonic stem cell

text: house mouse

text: targeted mutation

text: H3f3a knockout; H3f3b K79A

text: not applicable

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: frozen specimen


text: EMBL

text: public

characteristic: SRA accession

text: ArrayExpress

text: ESC 129-B13

text: mouse embryonic stem cell

text: house mouse

text: targeted mutation

text: H3f3a knockout; H3f3b wild type genotype

text: not applicable

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: frozen specimen


text: EMBL

text: public

characteristic: Submitter Id

text: ArrayExpress

text: ESC 129-B13

text: mouse embryonic stem cell

text: house mouse

text: targeted mutation

text: not applicable

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: frozen specimen


text: EMBL

text: public

text: ArrayExpress

characteristic: broker name

text: ESC 129-B13

text: mouse embryonic stem cell

text: house mouse

text: targeted mutation

text: not applicable

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: frozen specimen


text: EMBL

text: public

text: ArrayExpress

text: ESC 129-B13

text: mouse embryonic stem cell

text: house mouse

characteristic: common name

text: Protocols: Mouse embryonic stem cells (129XC57BL/6J) were cultured in ESC medium containing Knockout-DMEM (Thermo Fisher Scientific) supplemented with 15% EmbryoMax fetal bovine serum (ES-FBS Merck-Millipore) and 20 ng/ml leukemia inhibitory factor (LIF, produced by Protein Expression and Purification Facility at EMBL Heidelberg), 1% non-essential amino acids (Thermo Fisher Scientific), 1% Glutamax (Thermo Fisher Scientific), 1% Pen/Strep (Thermo Fisher Scientific), 1% of 55 mM beta-Mercaptoethanol solution (Merck Millipore). Upon thawing, cells were cultured on a layer of mouse embryonic fibroblasts (MEFs) and afterwards passaged on 0.1% gelatine-coated culture dishes, without feeder cells. Cells were maintained at 37°C with 5% CO2 and routinely tested for mycoplasma. The medium was changed daily, and cells were passed every two days. Precision nuclear run-on transcription sequencing (PRO-seq) experiments were performed following the protocol previously described (PMID: 27442863). PRO-seq was performed in two batches, one with two replicates each of control and H3.3K27A mESCs and one with three replicates each of control and H3.3K9A mESCs. Cells were harvested by scraping and permeabilized on ice for 10 min in permeabilization buffer, followed by 1 wash with the permeabilization buffer and centrifugations at 1000x g at 4°C. Cells were DAPI-stained, counted and resuspended in storage buffer. Aliquots were snap-frozen and stored at -80°C. For the experiment, 10 million mESCs were used for each reaction and 5% (i.e., 500k) of permeabilized Drosophila S2 cells were  added prior to the in-vitro run-on reaction. Run-on reactions were carried out by adding 100 µl of 2× NRO reaction mixture (10 mM of Tris-HCl, pH 8.0; 5 mM MgCl2; 300 mM KCl; 1 mM dithiothreitol (DTT); 1% sarkosyl; 375 µM biotin-11-CTP/-UTP; 375  µM ATP/GTP; 2 µl RNase inhibitor) to 100 µl of the permeabilized cells, and incubated at 30 °C for 3 min. Addition of TRIzol LS (Thermo Fisher Scientific) terminated the reaction and RNA was extracted and precipitated in 75% ethanol. Nuclear RNA was fragmented by base hydrolysis in 0.2 N of NaOH on ice for 10 min and subsequently neutralized by adding the same volume of 1 M of Tris-HCl, pH 6.8. Fragmented biotinylated RNA was bound to the streptavidin magnetic beads (Thermo Fisher Scientific), washed, eluted using TRIzol and precipitated in ethanol. Following 3′–RNA and 5′–RNA adapter ligation, the precipitated RNA was reverse-transcribed and PCR-amplified. DNA libraries were purified using SPRI-select beads and quality was assessed on a Bioanalyzer using the Agilent High Sensitivity DNA Kit.

text: targeted mutation

text: H3f3a knockout; H3f3b K9A

text: not applicable

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: fresh specimen


text: EMBL

text: public

text: ArrayExpress

text: ESC 129-B13

text: mouse embryonic stem cell

text: house mouse

characteristic: description

text: targeted mutation

text: H3f3a knockout; H3f3b K27A

text: not applicable

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: fresh specimen


text: EMBL

text: public

text: ArrayExpress

text: ESC 129-B13

text: mouse embryonic stem cell

text: house mouse

text: targeted mutation

characteristic: genetic modification

text: not applicable

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: fresh specimen


text: CHRISTIAN MEDICAL COLLEGE

characteristic: collection date

text: Genome Surveillance of  K. pneumoniae  from livestock specimens and hospital sewage

text: India

characteristic: geographic location (country and/or sea)

text: diseased

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: Blood

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: CHRISTIAN MEDICAL COLLEGE

text: India

text: diseased

text: Homo sapiens

characteristic: isolate

text: Blood

text: Klebsiella pneumoniae

characteristic: organism

text: Klebsiella pneumoniae

text: Genome Surveillance of K. pneumoniae from livestock specimens and hospital sewage

characteristic: title


characteristic: ENA-CHECKLIST

text: CHRISTIAN MEDICAL COLLEGE

text: India

text: diseased

text: Homo sapiens

text: Blood

text: Klebsiella quasipneumoniae

text: Klebsiella quasipneumoniae


text: CHRISTIAN MEDICAL COLLEGE

characteristic: INSDC center name

text: India

text: diseased

text: Homo sapiens

text: Blood

text: Klebsiella aerogenes

text: Klebsiella aerogenes


text: CHRISTIAN MEDICAL COLLEGE

text: India

text: diseased

text: Homo sapiens

text: Sputum

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


characteristic: ENA first public

characteristic: External Id

text: Cucumber

text: Cucumis sativus

text: RMCA

characteristic: INSDC last update

text: public

text: melon fly

text: larvae

text: third instar

text: gut

characteristic: environment (feature)

text: gut

characteristic: environment (material)

text: Tanzania

text: Zeugodacus cucurbitae

text: Zeugodacus cucurbitae

text: illumina

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene


text: Watermelon

text: Citrullus lanatus

text: RMCA

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: melon fly

characteristic: common name

text: larvae

characteristic: description

text: third instar

text: gut

text: gut

text: Tanzania

text: Zeugodacus cucurbitae

text: Zeugodacus cucurbitae

text: illumina

text: 16S rRNA


text: Sothern Medical University

text: public

characteristic: collection date

text: sample from human gut metagenome

text: China

characteristic: geographic location (country and/or sea)

text: human gut metagenome

text: human gut metagenome


text: public

tag: Namespace:University of Zagreb, Faculty of Science

text: 18S V4 rRNA gene sequences obtained from the skin of loggerhead sea turtle ID047

text: mediterranean sea biome

characteristic: environment (biome)

text: marine biota

text: biofilm material

text: Croatia

text: not collected

unit: DD

characteristic: geographic location (latitude)

text: not collected

characteristic: geographic location (longitude)

text: loggerhead sea turtle

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: metagenome

characteristic: investigation type

text: sequencing batch 2

characteristic: miscellaneous parameter

text: epibiont metagenome

characteristic: organism

characteristic: project name

text: epibiont metagenome

text: Illumina MiSeq

text: loggerhead skin sample TB116 18S V4 rRNA gene sequences

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-09-03-2022-15:03:55:799-146759

tag: Namespace:Institute for Chemistry and Biology of the Marine Environment

characteristic: Submitter Id

text: North Sea spring bloom; size-fraction 0.2-3µm

text: ocean

text: North Sea

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: North Sea

unit: m

characteristic: geographic location (depth)

characteristic: nucleic acid amplification

text: marine metagenome

characteristic: pcr primers

text: EcoMol Planktoron Amplicon Sequencing

text: marine metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: water

characteristic: water environmental package


text: Institute for Chemistry and Biology of the Marine Environment

text: public

text: ena-SAMPLE-TAB-09-03-2022-15:03:55:799-146760

characteristic: collection date

text: North Sea spring bloom; size-fraction 3-8µm

characteristic: description

text: ocean

characteristic: environment (biome)

text: North Sea

text: water

text: North Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: investigation type

text: marine metagenome

characteristic: organism

text: EcoMol Planktoron Amplicon Sequencing

characteristic: project name

text: marine metagenome

text: Illumina MiSeq

text: water


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-09-03-2022-15:03:55:799-146761

characteristic: Submitter Id

text: ocean

text: North Sea

text: water

text: North Sea

text: marine metagenome

text: EcoMol Planktoron Amplicon Sequencing

text: marine metagenome

text: Illumina MiSeq

characteristic: title

text: water


text: public

text: ena-SAMPLE-TAB-09-03-2022-15:03:55:799-146762

characteristic: collection date

characteristic: description

text: ocean

text: North Sea

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: North Sea

characteristic: geographic location (depth)

characteristic: nucleic acid amplification

text: marine metagenome

characteristic: pcr primers

text: EcoMol Planktoron Amplicon Sequencing

text: marine metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: water

characteristic: water environmental package


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: public

text: ena-SAMPLE-TAB-09-03-2022-15:03:55:799-146763

text: ocean

characteristic: environment (biome)

text: North Sea

text: water

text: North Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: investigation type

text: marine metagenome

characteristic: organism

text: EcoMol Planktoron Amplicon Sequencing

characteristic: project name

text: marine metagenome

text: Illumina MiSeq

text: water


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-09-03-2022-15:03:55:799-146764

characteristic: Submitter Id

characteristic: collection date

characteristic: description

text: ocean

text: North Sea

text: water

text: North Sea

text: marine metagenome

text: EcoMol Planktoron Amplicon Sequencing

text: marine metagenome

text: Illumina MiSeq

characteristic: title

text: water


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: public

text: ena-SAMPLE-TAB-09-03-2022-15:03:55:799-146765

text: ocean

text: North Sea

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: North Sea

characteristic: geographic location (depth)

characteristic: nucleic acid amplification

text: marine metagenome

characteristic: pcr primers

text: EcoMol Planktoron Amplicon Sequencing

text: marine metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: water

characteristic: water environmental package


characteristic: External Id

characteristic: INSDC center name

text: public

text: ena-SAMPLE-TAB-09-03-2022-15:03:55:799-146766

text: ocean

characteristic: environment (biome)

text: North Sea

text: water

text: North Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: investigation type

text: marine metagenome

characteristic: organism

text: EcoMol Planktoron Amplicon Sequencing

characteristic: project name

text: marine metagenome

text: Illumina MiSeq

text: water


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-09-03-2022-15:03:55:800-146767

characteristic: Submitter Id

characteristic: collection date

characteristic: description

text: ocean

text: North Sea

text: water

text: North Sea

text: marine metagenome

text: EcoMol Planktoron Amplicon Sequencing

text: marine metagenome

text: Illumina MiSeq

characteristic: title

text: water


characteristic: External Id

characteristic: INSDC center name

text: public

text: ena-SAMPLE-TAB-09-03-2022-15:03:55:800-146768

text: ocean

text: North Sea

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: North Sea

characteristic: geographic location (depth)

characteristic: nucleic acid amplification

text: marine metagenome

characteristic: pcr primers

text: EcoMol Planktoron Amplicon Sequencing

text: marine metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: water

characteristic: water environmental package


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: public

text: ena-SAMPLE-TAB-09-03-2022-15:03:55:800-146769

text: ocean

characteristic: environment (biome)

text: North Sea

text: water

text: North Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: investigation type

text: marine metagenome

characteristic: organism

text: EcoMol Planktoron Amplicon Sequencing

characteristic: project name

text: marine metagenome

text: Illumina MiSeq

text: water


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-09-03-2022-15:03:55:800-146770

characteristic: Submitter Id

characteristic: collection date

characteristic: description

text: ocean

text: North Sea

text: water

text: North Sea

text: marine metagenome

text: EcoMol Planktoron Amplicon Sequencing

text: marine metagenome

text: Illumina MiSeq

characteristic: title

text: water


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: public

text: ena-SAMPLE-TAB-09-03-2022-15:03:55:800-146771

text: ocean

text: North Sea

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: North Sea

characteristic: geographic location (depth)

characteristic: nucleic acid amplification

text: marine metagenome

characteristic: pcr primers

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