status: PUBLIC

characteristic: External Id

text: Antimicrobial Resistance Research Center, National Institute of Infectious Diseases

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: MIGS.ba.human-associated

characteristic: NCBI submission model

characteristic: NCBI submission package

characteristic: SRA accession

text: missing

tag: attribute

text: Keywords: GSC:MIxS;MIGS:6.0

characteristic: description

text: missing

text: missing

text: missing

text: Japan:Hiroshima

text: Homo sapiens

characteristic: host

text: missing

characteristic: isolate

text: missing

text: missing

text: Pseudomonas aeruginosa

characteristic: organism

text: Genome sequencing of Gram-negative antibiotic resistant bacteria collected in Hiroshima

text: missing

characteristic: strain

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Innstituto de Biomedicina de Valencia

text: public

tag: Namespace:Innstituto de Biomedicina de Valencia

characteristic: Submitter Id

text: WGS of M. tuberculosis samples from Mozambique 2018

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


characteristic: External Id

text: public

tag: Namespace:Genetics and Genomics of Insect Vectors Unit, Department of Parasites and Insect Vectors, Institut Pasteur, Universite de Paris Cite, CNRS UMR2000, F-75015 Paris, France

unit: day

characteristic: age

text: ArrayExpress

characteristic: broker name

text: Protocols: Three-day old female females Anopheles coluzzii mosquitoes were injected with dsPara or dsGFP, and after 4 days, total RNAs from three biological replicates were extracted and sequenced. For each biological replicate, pools of ~18 mosquitoes were extracted per treatment group. The para gene (AGAP004707), located on chromosome arm 2L, is composed of 39 exons, which transcribe 13 different messenger RNAs (mRNA) through alternate splicing. To maximize the efficacy of the gene silencing, two double stranded (ds) RNA for para were designed and synthesized for targeting each two distinct regions along the para gene with the aim to recognize all 13 transcripts. Each of the dsPara and the dsGFP used as control were prepared using the MEGAscriptTM RNAi Kit (Invitrogen by Thermo Fisher Scientific). Primers bearing the T7 RNA polymerase recognition sequence were designed to amplify ~604 (Para1) and ~730 (Para2) base pairs regions. For gene silencing procedure, one to three day old cold-anaesthetized mosquitoes were injected using the NanoJect II (Drummond) either with the equimolar mix of the two dsPara or with dsGFP for the control group. The mosquitoes were all injected into the thorax with approximately 1ug of dsRNA and maintained on sugar water until required for downstream experiments. Using Illumina’s Truseq RNA Sample Preparation Kit 1 microgram of total RNA was oligo-dT purified using oligo-dT coated magnetic beads, fragmented and then reverse transcribed into cDNA. The cDNA was fragmented, blunt-ended, and ligated to indexed (barcoded) adaptors and amplified using 15 cycles of PCR. Final library size distribution was validated using capillary electrophoresis and quantified using fluorimetry (PicoGreen) and via q-PCR. Indexed libraries were then normalized, pooled and then size selected to 320bp +/- 5% using Caliper’s XT instrument. Truseq libraries were hybridized to a paired end flow cell and individual fragments were clonally amplified by bridge amplification on the Illumina cBot.

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: pool of 18 mosquitoes

characteristic: individual

text: not applicable

text: Anopheles coluzzii

text: Anopheles coluzzii

text: whole organism

characteristic: organism part

text: Anopheles coluzzii

text: female

characteristic: sex

text: M


text: Genetics and Genomics of Insect Vectors Unit, Department of Parasites and Insect Vectors, Institut Pasteur, Universite de Paris Cite, CNRS UMR2000, F-75015 Paris, France

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

characteristic: description

text: adult

text: wild type genotype

text: pool of 18 mosquitoes

text: not applicable

text: Anopheles coluzzii

text: Anopheles coluzzii

text: whole organism

text: Anopheles coluzzii

text: female

text: M


text: Plasmodium falciparum

characteristic: ArrayExpress-SPECIES

text: SC

text: public

text: not provided

characteristic: collection date

text: malaria parasite P. falciparum

characteristic: common name

text: not provided

characteristic: geographic location (country and/or sea)

text: Plasmodium falciparum

characteristic: organism

text: Plasmodium falciparum

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: University of Crete

text: public

tag: Namespace:University of Crete

characteristic: Submitter Id

text: western European house mouse

text: mrna of wt1

text: Greece

text: Mus musculus domesticus

text: Mus musculus domesticus

text: Wild type 1


text: University of Crete

text: public

text: western European house mouse

text: mrna of ko4

text: Greece

text: Mus musculus domesticus

text: Mus musculus domesticus

text: Knock out 4


text: University of Crete

text: public

text: western European house mouse

text: mrna of ko3

text: Greece

text: Mus musculus domesticus

text: Mus musculus domesticus

text: Knock out 3


text: University of Crete

text: public

text: western European house mouse

text: mrna of ko2

text: Greece

text: Mus musculus domesticus

text: Mus musculus domesticus

text: Knock out 2


text: University of Crete

text: public

text: western European house mouse

text: mrna of ko1

text: Greece

text: Mus musculus domesticus

text: Mus musculus domesticus

text: Knock out 1


text: University of Crete

text: public

text: western European house mouse

text: mrna of wt1b

text: Greece

text: Mus musculus domesticus

text: Mus musculus domesticus

text: Wild type 1b


text: University of Crete

text: public

text: western European house mouse

text: mrna of wt4

text: Greece

text: Mus musculus domesticus

text: Mus musculus domesticus

text: Wild type 4


text: University of Crete

text: public

text: western European house mouse

text: mrna of wt2

text: Greece

text: Mus musculus domesticus

text: Mus musculus domesticus

text: Wild type 2


text: public

tag: Namespace:ErasmusMC, university medical center Roterdam

text: human

text: Hepatocellular carcinoma cell line Hep3B, AXIN-1 repaired clone A8

text: Netherlands

text: Homo sapiens

text: Homo sapiens

text: liver


text: ErasmusMC, university medical center Roterdam

text: public

text: human

text: Hepatocellular carcinoma cell line Hep3B, parental control cell line

text: Netherlands

text: Homo sapiens

text: Homo sapiens

text: liver


text: public

text: human

text: Hepatocellular carcinoma cell line Hep3B, AXIN-1 repaired clone F10

text: Netherlands

text: Homo sapiens

text: Homo sapiens

text: liver


text: public

text: human

text: Hepatocellular carcinoma cell line Hep3B, AXIN-1 repaired clone G9

text: Netherlands

text: Homo sapiens

text: Homo sapiens

text: liver


text: public

text: human

text: Hepatocellular carcinoma cell line HuH1, AXIN-1 repaired clone 2B3

text: Netherlands

text: Homo sapiens

text: Homo sapiens

text: liver


text: public

text: human

text: Hepatocellular carcinoma cell line HuH1, AXIN-1 repaired clone 2G5

text: Netherlands

text: Homo sapiens

text: Homo sapiens

text: liver


text: public

text: human

text: Hepatocellular carcinoma cell line HuH1, AXIN-1 repaired clone 2H3

text: Netherlands

text: Homo sapiens

text: Homo sapiens

text: liver


text: public

text: human

text: Hepatocellular carcinoma cell line HuH1, AXIN-1 repaired clone 2H5

text: Netherlands

text: Homo sapiens

text: Homo sapiens

text: liver


text: public

text: human

text: Hepatocellular carcinoma cell line, parental control cell line

text: Netherlands

text: Homo sapiens

text: Homo sapiens

text: liver


text: public

text: human

text: Hepatocellular carcinoma cell line JHH6, AXIN-1 repaired clone C12

text: Netherlands

text: Homo sapiens

text: Homo sapiens

text: liver


text: public

text: human

text: Hepatocellular carcinoma cell line JHH6, parental control cell line

text: Netherlands

text: Homo sapiens

text: Homo sapiens

text: liver


text: public

text: human

text: Hepatocellular carcinoma cell line JHH6, AXIN-1 repaired clone E12

text: Netherlands

text: Homo sapiens

text: Homo sapiens

text: liver


text: public

text: human

text: Hepatocellular carcinoma cell line JHH7, AXIN-1 repaired clone A6

text: Netherlands

text: Homo sapiens

text: Homo sapiens

text: liver


text: public

text: human

text: Hepatocellular carcinoma cell line JHH7, AXIN-1 repaired clone A8

text: Netherlands

text: Homo sapiens

text: Homo sapiens

text: liver


text: public

text: human

text: Hepatocellular carcinoma cell line JHH7, AXIN-1 repaired clone B9

text: Netherlands

text: Homo sapiens

text: Homo sapiens

text: liver


text: public

text: human

text: Hepatocellular carcinoma cell line JHH7, parental control cell line

text: Netherlands

text: Homo sapiens

text: Homo sapiens

text: liver


text: public

text: human

text: Hepatocellular carcinoma cell line SNU423, AXIN-1 repaied clone 2B10

text: Netherlands

text: Homo sapiens

text: Homo sapiens

text: liver


text: ErasmusMC, university medical center Roterdam

text: public

text: human

text: Hepatocellular carcinoma cell line SNU423, AXIN-1 repaied clone 2D3

text: Netherlands

text: Homo sapiens

text: Homo sapiens

text: liver


text: public

text: human

text: Hepatocellular carcinoma cell line SNU423, parental control cell line

text: Netherlands

text: Homo sapiens

text: Homo sapiens

text: liver


text: public

tag: Namespace:Charles University, Faculty of Science Department of Cell Biology

text: ArrayExpress

characteristic: broker name

text: not collected

text: fission yeast

text: Protocols: For Cbf11-related analyses, 400 mL cultures of Cbf11-TAP (MP707), Cbf11DBM-TAP (MP712), Cbf11-TAP_mga2KO (MP921), TAP-Mga2 (MP872), TAP-Mga2_cbf11KO (MP915), untagged WT (JB32, a negative control) and Fkh2-TAP (MP931, forkhead transcription factor unrelated to CSL; a negative control) cells were grown in YES to exponential phase. For Cbf12-related analyses, 10-15 mL of Cbf12-TAP (MP540), Cbf12DBM-TAP (MP518), untagged WT (JB32) and Fkh2-TAP (MP931) cells were grown in YES to stationary phase and were brought up to 400 mL with YES before fixation. Three independent biological experiments were performed for the CSL proteins; the negative controls were grown once for each cultivation condition. Saccharomyces cerevisiae cells expressing the histone H2A.2-TAP (Euroscarf yeast strain collection, original ID SC0421, lab ID MP792) were used as a potential spike-in control for normalization of ChIP samples of TAP-tagged S. pombe proteins. S. cerevisiae cells were cultivated to exponential phase (OD600 1.8) in the complex YPAD medium at 30°C, and chromatin extract preparation was performed as described below for S. pombe with these exceptions: fixation for 30 min, 15 cycles of chromatin shearing. However, after assessing the performance of the spike-in control, we decided not to take it into account during subsequent data analyses. Cells were fixed by adding formaldehyde to the final concentration of 1%. After 10 min incubation, the remaining formaldehyde was quenched by 125 mM glycine for 10 min. Cells were washed with PBS and broken with glass beads in the Lysis Buffer (50 mM Hepes pH 7.6, 1 mM EDTA pH 8.0, 150 mM NaCl, 1% Triton X-100, 0.1% sodium deoxycholate, FY protease inhibitors [Serva], 1 mM PMSF) using the FastPrep24 machine (MP Biomedicals). Extracted chromatin was sheared with the Bioruptor sonicator (Diagenode) using 20 cycles of 30 s on, 30 s off at high power settings. An aliquot of each chromatin extract was kept as the input control sample (used for a qPCR validation step), and 1.5-2 mg of chromatin extract with added S. cerevisiae spike-in chromatin (40,000-fold dilution) were used for immunoprecipitation with 100 μl of BSA-blocked magnetic IgG-coated beads (Invitrogen, 110.41). Chromatin immunoprecipitation was performed on a rotating wheel overnight at 4°C. The precipitated material was ChIP-exo treated according to the updated, simplified version of the ChIP-nexus approach (ChIP-nexus [version 2019]; https://research.stowers.org/zeitlingerlab/protocols.html) introduced by (He et al. 2015), except for wash buffers A-D that were optimized for yeasts as previously described (Doris et al. 2018). ChIP-exo treated material was eluted in Elution Buffer (50 mM Tris-HCl pH 8.0, 10 mM EDTA, 1% SDS). Cross-links were reversed at 65°C for 6 h, samples were treated with DNase-free RNase A followed by proteinase K digestion, and purified using standard phenol-chloroform extraction and sodium acetate/ethanol precipitation. qPCR was performed to check the efficiency of ChIP. ChIP-nexus library construction for Illumina sequencing was performed as described in the updated version of the ChIP-nexus approach (ChIP-nexus [version 2019]; https://research.stowers.org/zeitlingerlab/protocols.html). Concentration of ChIP-nexus libraries was measured using the Quantus fluorometer (Promega) and fragment size distribution was checked on Agilent Bioanalyzer using the High Sensitivity DNA Assay.

text: exponential phase

characteristic: developmental stage

characteristic: genotype

text: not collected

text: YES medium

characteristic: growth condition

text: not applicable

characteristic: isolate

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: whole organism

characteristic: organism part

text: Schizosaccharomyces pombe

text: added

characteristic: spike in

characteristic: strain


text: Charles University, Faculty of Science Department of Cell Biology

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: not collected

characteristic: collection date

text: fission yeast

characteristic: description

text: exponential phase

text: not collected

text: YES medium

text: not applicable

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: whole organism

text: Schizosaccharomyces pombe

text: added


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: public

text: ArrayExpress

text: not collected

text: fission yeast

characteristic: common name

text: stationary phase

text: not collected

characteristic: geographic location (country and/or sea)

text: YES medium

text: not applicable

text: Schizosaccharomyces pombe

characteristic: organism

text: Schizosaccharomyces pombe

text: whole organism

text: Schizosaccharomyces pombe

text: added

characteristic: title


characteristic: External Id

text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: not collected

text: fission yeast

text: exponential phase

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: not collected

text: YES medium

characteristic: growth condition

text: not applicable

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: whole organism

text: Schizosaccharomyces pombe


characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: ArrayExpress

text: not collected

text: fission yeast

text: stationary phase

text: wild type genotype

text: not collected

text: YES medium

text: not applicable

characteristic: isolate

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: whole organism

characteristic: organism part

text: Schizosaccharomyces pombe

text: added

characteristic: spike in

characteristic: strain


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: not collected

characteristic: collection date

text: fission yeast

text: Protocols: Samples were prepared from 3 biological replicates of mga2Δ (MP815) and mga2Δcbf11Δ (MP836) cells. Cells were cultured to exponential phase in the complex YES medium (with or without ammonium supplementation), 10 mL were harvested (600 g, 2 min) and the cell pellet was flash-frozen. Total RNA was isolated using a hot acidic phenol method followed by phenol-chloroform extractions and precipitation (PMID: 12854975). Extracted RNA was treated with TURBO DNase (Thermo Fisher Scientific) and purified using Qiagen RNeasy columns. RNA quality was assessed on Agilent Bioanalyzer. The sequencing libraries were synthesized using KAPA mRNA HyperPrep Kit (Illumina platform) (Roche, KK8581).

characteristic: description

text: not collected

text: YES medium

text: not applicable

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: whole organism

characteristic: replicate

text: Schizosaccharomyces pombe


characteristic: External Id

text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: not collected

text: fission yeast

characteristic: common name

text: not collected

characteristic: geographic location (country and/or sea)

text: YES medium with ammonium chloride

text: not applicable

text: Schizosaccharomyces pombe

characteristic: organism

text: Schizosaccharomyces pombe

text: whole organism

text: Schizosaccharomyces pombe

characteristic: title


characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: ArrayExpress

text: not collected

text: fission yeast

text: Protocols: At OD 0.5 10 ml aliquots of yeast cultures were centrifuged for 2 minutes at 600g. Supernatant was discarded. Pellets were flash-frozen in liquid nitrogen and placed in storage at -75°C. Cultures were pre-grown in YES to the exponentiation phase at 32°C. Then two aliquots of each cultures were centrifuged for 3' at 1000g, washed with YES and YES+93.5mM ammonium chloride (in parallel), diluted to OD=0.005 (WT) or 0.03 (cbf11Δ) and grown in corresponding media at 32°C. Caution: always use gloves, separate solutions and special tubes/tips when working with RNA 1. Harvest cells (usually 10 ml of OD600 ~0.5, adjust volume according to OD). Centrifuge 2’ at 600 g in a falcon tube and discard supernatant (SN). Snap freeze pellet (liquid nitrogen or dry ice/ethanol). Alternatively, filter cells and snap freeze filter disc. Store cells at -80°C. 2. Thaw cells on ice (~5 min). Add 1 ml of ice-cold nuclease-free (NF) water, resuspend cells, and transfer to 2ml Eppendorf tubes. Spin 10’’ at 2300 g and remove SN. Return tubes on ice.3. To pellet add 750 µl of TES (adjust if total cells are >5 ODs), resuspend cells with pipette, immediately add 750 µl acidic phenol-chloroform (refrigerated, Sigma P-1944 or P4682+C0549 5:1 mix; under fume hood), vortex, and place in 65°C heat block (use fume hood!). Only then process the next sample in the same way. 4. Incubate all samples in 65°C heat block for 1 hr, vortex 10’’ every 10’. 5. Place samples on ice for 1’, vortex 20’’, and centrifuge for 15’ at 20,000 g at 4°C. 6. Pre-spin 2ml MaXtract™ High Density tubes (QIAGEN, #129056; 1 tube per sample) for 10’’. Add 700 µl of acidic phenol-chloroform (under fume hood). 7. Take 700 µl of the water phase from step 5 and add to the MaXtract™ tubes from step 6, thoroughly mix by inverting (no vortexing), and centrifuge 5’ at 20,000 g at 4°C. 8. Pre-spin new 2ml MaXtract™ tubes as in step 6. Add 700 µl of chloroform:isoamyl alcohol (24:1, under fume hood, Sigma C-0549). 9. Take 700 µl of the water phase from step 7 and add to the MaXtract™ tubes from step 8, mix thoroughly by inverting (no vortexing), and centrifuge 5’ at 20,000 g at 4°C. 10. Prepare normal 2ml Eppendorf tubes (1 tube per sample) with 1.5 ml of 100% EtOH and 50 µl of 3M NaAc pH 5.2. 11. Transfer 500 µl of water phase from step 9 to the tubes from step 10, vortex 10’’. Samples can be precipitated at -20°C overnight (or at -80°C for 30 min). 12. Centrifuge for 10’ at 20,000 g at RT. Discard SN, add 500 µl 70% EtOH (4°C, made with NF water), don’t vortex, just add, and spin for 1’ (same tube orientation!). Aspirate most SN, spin 5’, and remove the rest of liquid with pipette. Air dry 5’’ at RT. 13. Add 100 µl of NF water, and incubate 1’ at 65°C. Dissolve pellet first by pipetting up and down (~30x) until no particles are left, then gently vortex 10’’.14. Measure concentrations with NanoDrop.15. Treat with TURBO DNase (ThermoFisher AM2238, see appendix 1).16. Clean with RNeasy columns (QIAgen74104). Elution with TE (see appendix 2, note the differences from the kit manual!).17. OPTIONAL: Run 10 µl of purified RNA on a 1% agarose gel (wipe gel apparatus/tray with RNAse-Zap, rinse with water and use new TBE buffer, use RNAse-free loading buffer). You should see the two ribosomal bands clean, distinct and without smears.18. Send an aliquot to Qubit and Bioanalyzer - 5ul in strips.TES: 10mM Tris pH 7.5; 10mM EDTA pH 8; 0.5% SDS (store at RT)Appendix 1. RNase treatment1. Add 10ug of RNA according to NanoDrop data.2. Add 5ul of 10x TURBO DNase Buffer.3. Add 1ul (2U) of TURBO DNase.4. Adjust volume to 50ul with RNase-free water. Mix by pipetting.5. Incubate 37°C 30’. Immediately proceed with column purification.Appendix 2. Column purification1. Adjust the sample to a volume of 100 μl with RNAse-free water. Add 350 μl Buffer RLT, and mix well.2. Add 250 μl ethanol (96–100%) to the diluted RNA, and mix well by pipetting. Do not centrifuge. Proceed immediately to step 3.3. Transfer the sample (700 μl) to an RNeasy Mini spin column placed in a 2 ml collection tube (supplied). Close the lid gently, and centrifuge for 15 s at ≥8000 x g (≥10,000 rpm). Discard the flow-through. Reuse the collection tube in step 4.Note: After centrifugation, carefully remove the RNeasy spin column from the collection tube so that the column does not contact the flow-through. Be sure to empty the collection tube completely.4. Add 500 μl Buffer RPE to the RNeasy spin column. Close the lid gently, and centrifuge for 15 s at ≥8000 x g (≥10,000 rpm) to wash the spin column membrane. Discard the flow-through.Reuse the collection tube in step 5. Note: Ensure that ethanol is added to Buffer RPE before use.5. Add 500 μl Buffer RPE to the RNeasy spin column. Close the lid gently, and centrifuge for 2 min at ≥8000 x g (≥10,000 rpm) to wash the spin column membrane.Note: After centrifugation, carefully remove the RNeasy spin column from the collection tube so that the column does not contact the flow-through. Otherwise, carryover of ethanol will occur.6. Optional: Place the RNeasy spin column in a new 2 ml collection tube (supplied), and discard the old collection tube with the flow-through. Close the lid gently, and centrifuge at full speed for 1 min.Perform this step to eliminate any possible carryover of Buffer RPE, or if residual flow-through remains on the outside of the RNeasy spin column after step 5.7. Place the RNeasy spin column in a new 1.5 ml collection tube (supplied). Add 50 μl of TE buffer directly to the spin column membrane. Close the lid gently, leave for 5’ at RT. Centrifuge for 1 min at ≥8000 x g (≥10,000 rpm) to elute the RNA.8. Collect the flow-through and load it back on the membrane. Centrifuge for 1 min at ≥8000 x g (≥10,000 rpm) to elute the RNA. The Illumina TruSeq stranded mRNA Library (poly-A) kit was used for library construction using the manufacturer's instructions.

text: Δcbf11::kanR, h+

characteristic: genotype

text: not collected

text: YES medium with ammonium chloride

characteristic: growth condition

text: not applicable

characteristic: isolate

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: whole organism

characteristic: organism part

text: Schizosaccharomyces pombe


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: not collected

text: fission yeast

text: Δcbf11::kanR, h+

text: not collected

text: YES medium

text: not applicable

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: whole organism

text: Schizosaccharomyces pombe

characteristic: strain


characteristic: External Id

text: public

characteristic: Submitter Id

text: ArrayExpress

text: not collected

characteristic: collection date

text: fission yeast

characteristic: description

text: wild type genotype

text: not collected

text: YES medium with ammonium chloride

text: not applicable

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: whole organism

characteristic: replicate

text: Schizosaccharomyces pombe


text: public

text: ArrayExpress

characteristic: broker name

text: not collected

text: fission yeast

characteristic: common name

text: wild type genotype

text: not collected

characteristic: geographic location (country and/or sea)

text: YES medium

text: not applicable

text: Schizosaccharomyces pombe

characteristic: organism

text: Schizosaccharomyces pombe

text: whole organism

text: Schizosaccharomyces pombe

characteristic: title


characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: ArrayExpress

text: not collected

text: fission yeast

text: Protocols: Samples were prepared from 3 biological replicates. Two separate experiments were performed: 1) Cells were cultured to exponential phase in the complex YES medium with or without ammonium supplementation. 2) Cells were cultured to exponential phase in the complex YES medium and then treated with DMSO (control) or 20 μM cerulenin for 1 hour. For each sample, 10 mL were harvested (600 g, 2 min) and the cell pellet was flash-frozen. Total RNA was isolated using a hot acidic phenol method followed by phenol-chloroform extractions and precipitation (PMID: 12854975). Extracted RNA was treated with TURBO DNase (Thermo Fisher Scientific) and purified using Qiagen RNeasy columns. RNA quality was assessed on Agilent Bioanalyzer. The sequencing libraries were synthesized using KAPA mRNA HyperPrep Kit (Illumina platform) (Roche, KK8581).

text: wild type genotype

characteristic: genotype

text: not collected

text: YES medium

characteristic: growth condition

text: not applicable

characteristic: isolate

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: whole organism

text: Schizosaccharomyces pombe


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: not collected

text: fission yeast

text: not collected

text: YES medium

text: not applicable

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: whole organism

characteristic: organism part

text: Schizosaccharomyces pombe


characteristic: External Id

text: public

characteristic: Submitter Id

text: ArrayExpress

text: not collected

characteristic: collection date

text: fission yeast

characteristic: description

text: not collected

text: YES medium with ammonium chloride

text: not applicable

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: whole organism

text: Schizosaccharomyces pombe

characteristic: strain


text: Anopholes gambiae

characteristic: ArrayExpress-SPECIES

text: SC

text: public

text: not provided

text: African malaria mosquito

characteristic: common name

text: Burkina Faso

characteristic: geographic location (country and/or sea)

text: Anopheles gambiae

characteristic: organism

text: Anopheles gambiae


text: University of Crete

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: western European house mouse

text: Total RNA

text: Greece

text: Mus musculus domesticus

text: Mus musculus domesticus

text: Knock out 3.

characteristic: title


characteristic: ENA first public

text: University of Crete

text: public

text: western European house mouse

text: Total RNA

text: Greece

text: Mus musculus domesticus

text: Mus musculus domesticus

text: Knock out 2.


characteristic: ENA-CHECKLIST

text: University of Crete

text: public

text: western European house mouse

text: Total RNA

text: Greece

text: Mus musculus domesticus

text: Mus musculus domesticus

text: Knock out 1.


text: University of Crete

text: public

characteristic: INSDC status

text: western European house mouse

text: Total RNA

text: Greece

text: Mus musculus domesticus

text: Mus musculus domesticus

text: Wild type 3.


text: University of Crete

text: public

characteristic: SRA accession

text: western European house mouse

text: Total RNA

text: Greece

text: Mus musculus domesticus

text: Mus musculus domesticus

text: Wild type 2.


text: University of Crete

text: public

text: western European house mouse

text: Total RNA

text: Greece

text: Mus musculus domesticus

text: Mus musculus domesticus

text: Wild type 1.


text: GIMR

text: public

text: Richard Pillans

text: CSIRO

characteristic: collecting institution

text: common sawfish

text: Blood samples from pristis pristis

text: Australia

text: Archer river, Queensland

characteristic: geographic location (region and locality)

text: river/estuarine

characteristic: habitat

text: juvenile

characteristic: lifestage

text: Pristis pristis

text: blood

characteristic: organism part

text: ORG.one

characteristic: project name

text: Pristis pristis

text: male

characteristic: sex


E-MTAB-13923:Clone 0

text: Laboratory of Cancer Cell Biology, Institute of Molecular Genetics of the ASCR, Prague, Czech Republic

text: public

text: E-MTAB-13923:Clone 0

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: human

characteristic: common name

text: Protocols: non-transformed RPE-PPM1D-T1 cells and six RPE-PPM1D-T1-SA clones  Quick-gDNA Miniprep kit (Zymo Research) according to the manufacturer’s protocols KAPA EvoPlus Kit (Roche)

characteristic: description

characteristic: developmental stage

characteristic: genotype

text: not collected

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Clone 0


E-MTAB-13923:Clone 1

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: E-MTAB-13923:Clone 1

text: ArrayExpress

text: not collected

text: human

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: Clone 1

characteristic: title


E-MTAB-13923:Clone 2

text: public

characteristic: SRA accession

text: E-MTAB-13923:Clone 2

text: ArrayExpress

text: not collected

text: human

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Clone 2


E-MTAB-13923:Clone 3

text: public

text: E-MTAB-13923:Clone 3

text: ArrayExpress

text: not collected

text: human

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Clone 3


E-MTAB-13923:Clone 4

text: public

text: E-MTAB-13923:Clone 4

text: ArrayExpress

text: not collected

text: human

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Clone 4


E-MTAB-13923:Clone 5

text: public

text: E-MTAB-13923:Clone 5

text: ArrayExpress

text: not collected

text: human

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Clone 5


E-MTAB-13923:Clone 6

text: public

text: E-MTAB-13923:Clone 6

text: ArrayExpress

text: not collected

text: human

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Clone 6


text: Center for Oncological Research and Integrated Personalised Precision Oncology Network - Antwerp University

text: public

text: ArrayExpress

text: breast invasive ductal carcinoma cell

text: not collected

text: human

text: Protocols: Monolayer cells of SUM149, rSUM149, and rrSUM149 (n=3 for each cell line) seeded with their respective medium in 100 mm dishes (250,000 cells/dish) were harvested 72 hours after they were seeded. Monolayer cells were lifted using a cell scraper instead of trypsin-EDTA. After collection, monolayer cells were pelleted, washed with ice-cold PBS, and re-pelleted to be stored at -80°C until RNA extraction.  SUM149PT cells were obtained from Asterand Inc. and were routinely cultured in Ham’s F12 medium supplemented with 5% FBS, 1% penicillin/streptomycin, 1% antibiotic/antimycotic, hydrocortisone and an insulin/transferrin/selenium cocktail. To generate the resistance model rSUM149, SUM149 cells were routinely maintained in normal culture medium supplemented with 7.5 μM research grade Lapatinib in increasing concentrations (0.25– 7.5 μM) for a period of 3 months. Cells were grown for two weeks in each dosage concentration. Following an initial decrease in cell growth and cell death, small colonies of viable cells were seen by the end of the two weeks and were cultured to confluence before the next drug concentration increase. The resistance reversal model rrSUM149 was generated from rSUM149 after removal of Lapatinib >3 months and were cultured in regular SUM149 media. All cell lines were cultured at 37C with 5% CO2 for no more than 2 months prior to use in this study. Total RNA was extracted was conducted using Qiagen RNeasy Plus Universal mini kit following the manufacturer’s instructions. Extracted RNA samples were quantified using Qubit 2.0 Fluorometer and RNA integrity was checked using Agilent TapeStation 4200. RNA sequencing libraries (n=9) were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina following the manufacturer’s instructions. Briefly, mRNAs were enriched with Oligo(dT) beads and fragmented for 15 minutes at 94 °C. First strand and second strand cDNAs were subsequently synthesized. cDNA fragments were end repaired and adenylated at 3’ends, and universal adapters were ligated to cDNA fragments, followed by index addition and library enrichment by limited-cycle PCR. The sequencing libraries were validated on the Agilent TapeStation, and quantified by using Qubit 2.0 Fluorometer as well as by quantitative PCR.

text: adult

text: breast neoplasm

characteristic: disease

text: ERBB2 CNA

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

characteristic: organism part

text: Lapatinib resistance reversal

characteristic: phenotype

characteristic: replicate

text: Homo sapiens


text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: breast invasive ductal carcinoma cell

text: not collected

characteristic: collection date

text: human

characteristic: common name

text: adult

text: breast neoplasm

text: ERBB2 CNA

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens


text: public

text: ArrayExpress

text: breast invasive ductal carcinoma cell

text: not collected

text: human

characteristic: description

text: adult

characteristic: developmental stage

text: breast neoplasm

text: ERBB2 CNA

characteristic: genotype

text: not collected

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Lapatinib resistance

text: Homo sapiens


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: ArrayExpress

text: breast invasive ductal carcinoma cell

text: not collected

text: human

text: adult

text: breast neoplasm

text: ERBB2 CNA

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Control

text: Homo sapiens


E-MTAB-13933:Clone 0

text: public

characteristic: SRA accession

text: E-MTAB-13933:Clone 0

text: ArrayExpress

text: not collected

text: human

text: Protocols: non-transformed RPE-PPM1D-T1 cells and six RPE-PPM1D-T1-SA clones  RNeasy Mini Kit (Qiagen) according to the manufacturer’s protocols KAPA EvoPlus Kit (Roche)

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: Clone 0

characteristic: title


E-MTAB-13933:Clone 1

text: public

text: E-MTAB-13933:Clone 1

text: ArrayExpress

text: not collected

text: human

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Clone 1


E-MTAB-13933:Clone 2

text: public

text: E-MTAB-13933:Clone 2

text: ArrayExpress

text: not collected

text: human

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Clone 2


E-MTAB-13933:Clone 3

text: public

text: E-MTAB-13933:Clone 3

text: ArrayExpress

text: not collected

text: human

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Clone 3


E-MTAB-13933:Clone 4

text: public

text: E-MTAB-13933:Clone 4

text: ArrayExpress

text: not collected

text: human

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Clone 4


E-MTAB-13933:Clone 5

text: public

text: E-MTAB-13933:Clone 5

text: ArrayExpress

text: not collected

text: human

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Clone 5


E-MTAB-13933:Clone 6

text: public

text: E-MTAB-13933:Clone 6

text: ArrayExpress

text: not collected

text: human

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Clone 6


E-MTAB-13938:N2 2% agar T1

text: USC

text: public

text: E-MTAB-13938:N2 2% agar T1

text: ArrayExpress

text: not collected

text: Protocols: For RNA isolation, all RNa collection was performed at day 2 of adulthood. ~1000 animals were harvested from RNAi plates using M9. Animals were pelleted by gravity by allowing adult worms to settle to the bottom of the tube and aspirating off eggs and L1. Animals were washed and gravity settled 3x to remove a majority of progeny, then animals were placed into Trizol solution and worms were freeze/thawed 3x with liquid nitrogen with a 30 sec vortexing step between each freeze cycle.  Standard bleaching protocol was used to synchronize animal populations. Animals were L1 arrested for a maximum of 16 hours to further synchronize populations. Animals were grown on RNAi indicated from L1 until Day 1 of adulthood and material was collected on Day 1 of adulthood. Animals were either grown on 2% or 4% solid agar medium from L1 stage. After the final thaw, chloroform was added at a 1:5 chlorform/trizol ratio and aqueous separation of RNA was performed via centrifugation in a heavy gel phase-lock tube (VWR, 10847-802). The aqueous phase was mixed 1:1 with isopropanol then applied to a Qiagen RNeasy Mini Kit (74106) and RNA purification was performed as per manufacturer’s directions.  Library construction was performed by Novogene using their standard pipeline.

text: adult

text: wild type genotype

text: not collected

text: 2% solid agar medium

characteristic: growth condition

text: not applicable

text: Caenorhabditis elegans

text: Caenorhabditis elegans

characteristic: replicate

text: Caenorhabditis elegans

text: N2 2% agar T1


E-MTAB-13938:N2 2% agar T2

text: USC

text: public

text: E-MTAB-13938:N2 2% agar T2

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: not collected

text: adult

text: wild type genotype

text: not collected

text: 2% solid agar medium

text: not applicable

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: N2 2% agar T2


E-MTAB-13938:N2 2% agar T3

text: USC

text: public

text: E-MTAB-13938:N2 2% agar T3

text: ArrayExpress

text: not collected

characteristic: collection date

text: adult

text: wild type genotype

text: not collected

text: 2% solid agar medium

text: not applicable

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: N2 2% agar T3


E-MTAB-13938:N2 4% agar T1

text: USC

text: public

text: E-MTAB-13938:N2 4% agar T1

text: ArrayExpress

text: not collected

characteristic: description

text: adult

text: wild type genotype

text: not collected

text: 4% solid agar medium

text: not applicable

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: N2 4% agar T1


E-MTAB-13938:N2 4% agar T2

text: USC

text: public

text: E-MTAB-13938:N2 4% agar T2

text: ArrayExpress

text: not collected

text: adult

characteristic: developmental stage

text: wild type genotype

text: not collected

text: 4% solid agar medium

text: not applicable

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: N2 4% agar T2


E-MTAB-13938:N2 4% agar T3

text: USC

text: public

text: E-MTAB-13938:N2 4% agar T3

text: ArrayExpress

text: not collected

text: adult

text: wild type genotype

characteristic: genotype

text: not collected

text: 4% solid agar medium

text: not applicable

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: N2 4% agar T3


text: Gyeongsang National University Animal Genomics Laboratory

text: public

text: ArrayExpress

text: not collected

text: dog

characteristic: common name

text: Protocols: For whole genome sequencing, whole blood samples were collected from 113 Jindo dogs. The experiment was ethically approved by the National Institute of Animal Science, Rural Development Administration (approval no: NIAS2018-275). Total DNA was extracted from the trachea with TRIzol Reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s protocol. DNA concentration was checked using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, USA). Individual libraries were generated using Illumina TruSeq™ DNA Sample Preparation Kit (Illumina, San Diego, CA, USA).

text: not collected

text: not applicable

characteristic: isolate

text: Canis lupus familiaris

text: Canis lupus familiaris

text: blood

characteristic: organism part

text: Yellow

characteristic: phenotype

text: Canis lupus familiaris

text: Female

characteristic: sex


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: not collected

text: dog

text: not collected

text: not applicable

text: Canis lupus familiaris

text: Canis lupus familiaris

text: blood

text: Blacktan

text: Canis lupus familiaris

text: Female


text: public

text: ArrayExpress

text: not collected

text: dog

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

text: Canis lupus familiaris

characteristic: organism

text: Canis lupus familiaris

text: blood

text: White

text: Canis lupus familiaris

text: Male

characteristic: title


text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: dog

text: not collected

text: not applicable

text: Canis lupus familiaris

text: Canis lupus familiaris

text: blood

text: White

text: Canis lupus familiaris

text: Female


text: public

text: ArrayExpress

text: not collected

text: dog

characteristic: description

text: not collected

text: not applicable

text: Canis lupus familiaris

text: Canis lupus familiaris

text: blood

text: Yellow

text: Canis lupus familiaris

text: Male


text: public

text: ArrayExpress

text: not collected

text: dog

characteristic: common name

text: not collected

text: not applicable

text: Canis lupus familiaris

text: Canis lupus familiaris

text: blood

text: Black

text: Canis lupus familiaris


text: public

text: ArrayExpress

text: not collected

text: dog

text: not collected

text: not applicable

characteristic: isolate

text: Canis lupus familiaris

text: Canis lupus familiaris

text: blood

text: Brindle

text: Canis lupus familiaris


text: public

text: ArrayExpress

text: not collected

text: dog

text: not collected

text: not applicable

text: Canis lupus familiaris

text: Canis lupus familiaris

text: blood

characteristic: organism part

text: Black

text: Canis lupus familiaris

text: Female


text: public

text: ArrayExpress

text: not collected

text: dog

text: not collected

text: not applicable

text: Canis lupus familiaris

text: Canis lupus familiaris

text: blood

text: Black

characteristic: phenotype

text: Canis lupus familiaris

text: Male


text: public

text: ArrayExpress

text: not collected

text: dog

text: not collected

text: not applicable

text: Canis lupus familiaris

text: Canis lupus familiaris

text: blood

text: Blacktan

text: Canis lupus familiaris

characteristic: sex


characteristic: ENA first public

text: public

text: ArrayExpress

text: not collected

text: dog

text: not collected

text: not applicable

text: Canis lupus familiaris

text: Canis lupus familiaris

text: blood

text: Blacktan

text: Canis lupus familiaris

text: Male


characteristic: ENA-CHECKLIST

text: public

text: ArrayExpress

text: not collected

text: dog

text: not collected

text: not applicable

text: Canis lupus familiaris

text: Canis lupus familiaris

text: blood

text: Brindle

text: Canis lupus familiaris

text: Male


characteristic: INSDC center name

text: public

text: ArrayExpress

text: not collected

text: dog

text: not collected

text: not applicable

text: Canis lupus familiaris

text: Canis lupus familiaris

text: blood

text: Brindle

text: Canis lupus familiaris

text: Female


text: public

characteristic: INSDC status

text: ArrayExpress

text: not collected

text: dog

text: not collected

text: not applicable

text: Canis lupus familiaris

text: Canis lupus familiaris

text: blood

text: Gray

text: Canis lupus familiaris

text: Male


text: University of Chicago

text: public

characteristic: SRA accession

unit: week

characteristic: age

text: ArrayExpress

text: not collected

text: house mouse

text: Protocols: Live CD11b+ Ly6Chi cells were FACS sorted from wildtype B6 mice into Takara RNA isolation sort buffer containing RNase inhibitor and 3' Primer II A (as per Takara instructions)  RNA was isolated from sorted cells using the Takara SMART-Seq mRNA LP kit according to manufacturer directions.  RNA libraries were constructed using Takara SMART-Seq mRNA LP kit and Takara unique dual index kit according to manufacturer directions. Libraries were quantified, then equal quantity of library was pooled before sequencing

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: not collected

characteristic: individual

text: not applicable

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: female

characteristic: strain


text: The Kennel Club Genetics Centre

text: public

characteristic: Submitter Id

text: Pomeranian

characteristic: breed

characteristic: collection date

text: dog

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Canis lupus familiaris

characteristic: organism

text: Canis lupus familiaris

text: Male

text: Pomeranian WGS

characteristic: title


text: MAX PLANCK INSTITUTE FOR DEVELOPMENTAL BIOLOGY

text: public

text: DNA of Pristionchus pacificus RSC011 mutation accumulation line MAN_114

characteristic: description

text: Germany

text: Pristionchus pacificus

text: Pristionchus pacificus


characteristic: ENA first public

text: Haukeland University Hospital

text: public

text: Swab

text: Norway

text: diseased

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

characteristic: isolate

text: Skin wound

text: Streptococcus dysgalactiae

text: Streptococcus dysgalactiae


characteristic: ENA-CHECKLIST

text: OH-STAR

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E. coli

characteristic: common name

text: Canine Faeces

text: United Kingdom

text: Escherichia coli

text: Escherichia coli


text: Genoscope

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

text: public

text: GOCE UL 8b

text: COPO

characteristic: broker name

text: Tomasz Mamos, Michal Grabowski, Karolina Biniek, Piotr Falczewski

text: University of Lodz

characteristic: collecting institution

characteristic: collector ORCID ID

text: Germany

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Lobbe|Rugia

characteristic: geographic location (region and locality)

text: intertidal

characteristic: habitat

text: Michal Grabowski

text: University of Lodz

text: adult

characteristic: lifestage

text: Gammarus oceanicus

text: WHOLE ORGANISM

characteristic: organism part

text: ERGA

characteristic: project name

text: hand net

characteristic: sample collection method

text: Tomasz Mamos

characteristic: sample coordinator

characteristic: sample coordinator ORCID ID

text: University of Lodz

characteristic: sample coordinator affiliation

text: Gammarus oceanicus

text: MALE

characteristic: sex

text: ERGA GOC 0307 016

text: d7e2c716-b765-47d1-a9af-12c70b96bf42-ERGA-specimen

characteristic: tolid


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Genoscope

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: GOCE UL 8b

text: COPO

text: University of Lodz

characteristic: collection date

text: Germany

characteristic: geographic location (country and/or sea)

text: Lobbe|Rugia

text: intertidal

text: Michal Grabowski

text: University of Lodz

text: adult

text: Gammarus oceanicus

characteristic: organism

text: WHOLE ORGANISM

text: ERGA

text: hand net

text: Tomasz Mamos

text: University of Lodz

text: Gammarus oceanicus

text: MALE

text: ERGA GOC 0307 015

text: 304b20a2-9964-4449-b02e-77326fffda84-ERGA-specimen

characteristic: title


text: Genoscope

text: NOT PROVIDED

text: EarlhamInstitute

text: public

text: GOCE UL 7b

text: COPO

characteristic: broker name

text: University of Lodz

characteristic: collecting institution

characteristic: collector ORCID ID

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Lobbe|Rugia

characteristic: geographic location (region and locality)

text: intertidal

characteristic: habitat

text: Michal Grabowski

text: University of Lodz

text: adult

characteristic: lifestage

text: Gammarus oceanicus

text: WHOLE ORGANISM

characteristic: organism part

text: ERGA

characteristic: project name

text: hand net

characteristic: sample collection method

text: Tomasz Mamos

text: University of Lodz

text: Gammarus oceanicus

text: MALE

text: ERGA GOC 0307 014

text: 352e85e6-0cc7-4a80-a7e1-9bdc7a127f2d-ERGA-specimen


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Genoscope

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: GOCE UL 7b

text: COPO

text: University of Lodz

text: Germany

text: Lobbe|Rugia

text: intertidal

text: Michal Grabowski

text: University of Lodz

text: adult

text: Gammarus oceanicus

text: WHOLE ORGANISM

text: ERGA

text: hand net

text: Tomasz Mamos

characteristic: sample coordinator

characteristic: sample coordinator ORCID ID

text: University of Lodz

characteristic: sample coordinator affiliation

text: Gammarus oceanicus

text: MALE

characteristic: sex

text: ERGA GOC 0307 013

text: 1fe7e8ae-dd91-48c2-a7ae-056e1119a2fb-ERGA-specimen

characteristic: tolid


text: Genoscope

text: NOT PROVIDED

text: EarlhamInstitute

text: public

characteristic: Submitter Id

text: GOCE UL 6b

text: COPO

characteristic: broker name

text: University of Lodz

characteristic: collecting institution

characteristic: collection date

characteristic: collector ORCID ID

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Lobbe|Rugia

characteristic: geographic location (region and locality)

text: intertidal

text: Michal Grabowski

text: University of Lodz

text: adult

text: Gammarus oceanicus

characteristic: organism

text: WHOLE ORGANISM

text: ERGA

text: hand net

text: Tomasz Mamos

text: University of Lodz

text: Gammarus oceanicus

text: MALE

text: ERGA GOC 0307 012

text: 6531ef82-5998-442f-8e95-9eb18617aa5c-ERGA-specimen

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Genoscope

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: GOCE UL 6b

text: COPO

text: University of Lodz

text: Germany

text: Lobbe|Rugia

text: intertidal

characteristic: habitat

text: Michal Grabowski

text: University of Lodz

text: adult

characteristic: lifestage

text: Gammarus oceanicus

text: WHOLE ORGANISM

characteristic: organism part

text: ERGA

characteristic: project name

text: hand net

characteristic: sample collection method

text: Tomasz Mamos

text: University of Lodz

text: Gammarus oceanicus

text: MALE

text: ERGA GOC 0307 011

text: a9353daa-e0cc-4c22-b29d-1e1a6794ad05-ERGA-specimen


text: Genoscope

text: NOT PROVIDED

text: EarlhamInstitute

text: public

characteristic: Submitter Id

text: GOCE UL 5b

text: COPO

characteristic: broker name

text: University of Lodz

characteristic: collecting institution

characteristic: collection date

characteristic: collector ORCID ID

text: Germany

characteristic: geographic location (country and/or sea)

text: Boiensdorf|Wismar Bay

text: intertidal

text: Michal Grabowski

text: University of Lodz

text: adult

text: Gammarus oceanicus

text: WHOLE ORGANISM

text: ERGA

text: hand net

text: Tomasz Mamos

characteristic: sample coordinator

characteristic: sample coordinator ORCID ID

text: University of Lodz

characteristic: sample coordinator affiliation

text: Gammarus oceanicus

text: FEMALE

characteristic: sex

text: ERGA GOC 0307 010

text: 4e412841-a2d9-4408-ac75-031b21883277-ERGA-specimen

characteristic: tolid


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Genoscope

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: GOCE UL 5b

text: COPO

text: University of Lodz

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Boiensdorf|Wismar Bay

characteristic: geographic location (region and locality)

text: intertidal

text: Michal Grabowski

text: University of Lodz

text: adult

text: Gammarus oceanicus

characteristic: organism

text: WHOLE ORGANISM

text: ERGA

text: hand net

text: Tomasz Mamos

text: University of Lodz

text: Gammarus oceanicus

text: FEMALE

text: ERGA GOC 0307 009

text: 86c0a48c-59bd-455a-a2ae-89d07b081e66-ERGA-specimen

characteristic: title


text: Genoscope

text: NOT PROVIDED

text: EarlhamInstitute

text: public

characteristic: Submitter Id

text: GOCE UL 4b

text: COPO

characteristic: broker name

text: University of Lodz

characteristic: collecting institution

characteristic: collection date

characteristic: collector ORCID ID

text: Germany

characteristic: geographic location (country and/or sea)

text: Boiensdorf|Wismar Bay

text: intertidal

characteristic: habitat

text: Michal Grabowski

text: University of Lodz

text: adult

characteristic: lifestage

text: Gammarus oceanicus

text: WHOLE ORGANISM

characteristic: organism part

text: ERGA

characteristic: project name

text: hand net

characteristic: sample collection method

text: Tomasz Mamos

text: University of Lodz

text: Gammarus oceanicus

text: MALE

text: ERGA GOC 0307 008

text: ee1ddbea-c6b7-4275-b480-09b678f4ce03-ERGA-specimen


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Genoscope

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: GOCE UL 4b

text: COPO

text: University of Lodz

text: Germany

text: Boiensdorf|Wismar Bay

text: intertidal

text: Michal Grabowski

text: University of Lodz

text: adult

text: Gammarus oceanicus

text: WHOLE ORGANISM

text: ERGA

text: hand net

text: Tomasz Mamos

characteristic: sample coordinator

characteristic: sample coordinator ORCID ID

text: University of Lodz

characteristic: sample coordinator affiliation

text: Gammarus oceanicus

text: MALE

characteristic: sex

text: ERGA GOC 0307 007

text: b7bc4333-012e-4f4f-82eb-585a5151f00e-ERGA-specimen

characteristic: tolid


text: Genoscope

text: NOT PROVIDED

text: EarlhamInstitute

text: public

characteristic: Submitter Id

text: GOCE UL 3b

text: COPO

characteristic: broker name

text: University of Lodz

characteristic: collecting institution

characteristic: collection date

characteristic: collector ORCID ID

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Boiensdorf|Wismar Bay

characteristic: geographic location (region and locality)

text: intertidal

text: Michal Grabowski

text: University of Lodz

text: adult

text: Gammarus oceanicus

characteristic: organism

text: WHOLE ORGANISM

text: ERGA

text: hand net

text: Tomasz Mamos

text: University of Lodz

text: Gammarus oceanicus

text: MALE

text: ERGA GOC 0307 006

text: 1987dae1-cf65-4a8d-8e87-547215a8e2f1-ERGA-specimen

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Genoscope

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: GOCE UL 3b

text: COPO

text: University of Lodz

text: Germany

text: Boiensdorf|Wismar Bay

text: intertidal

characteristic: habitat

text: Michal Grabowski

text: University of Lodz

text: adult

characteristic: lifestage

text: Gammarus oceanicus

text: WHOLE ORGANISM

characteristic: organism part

text: ERGA

characteristic: project name

text: hand net

characteristic: sample collection method

text: Tomasz Mamos

text: University of Lodz

text: Gammarus oceanicus

text: MALE

text: ERGA GOC 0307 005

text: d119e986-6552-4641-989d-2f1066ec3391-ERGA-specimen


text: Genoscope

text: NOT PROVIDED

text: EarlhamInstitute

text: public

characteristic: Submitter Id

text: GOCE UL 2b

text: COPO

characteristic: broker name

text: University of Lodz

characteristic: collecting institution

characteristic: collection date

characteristic: collector ORCID ID

text: Germany

characteristic: geographic location (country and/or sea)

text: Boiensdorf|Wismar Bay

text: intertidal

text: Michal Grabowski

text: University of Lodz

text: adult

text: Gammarus oceanicus

text: WHOLE ORGANISM

text: ERGA

text: hand net

text: Tomasz Mamos

characteristic: sample coordinator

characteristic: sample coordinator ORCID ID

text: University of Lodz

characteristic: sample coordinator affiliation

text: Gammarus oceanicus

text: MALE

characteristic: sex

text: ERGA GOC 0307 004

text: 31666701-6260-402a-b668-e2a56c327068-ERGA-specimen

characteristic: tolid


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Genoscope

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: GOCE UL 2b

text: COPO

text: University of Lodz

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Boiensdorf|Wismar Bay

characteristic: geographic location (region and locality)

text: intertidal

text: Michal Grabowski

text: University of Lodz

text: adult

text: Gammarus oceanicus

characteristic: organism

text: WHOLE ORGANISM

text: ERGA

text: hand net

text: Tomasz Mamos

text: University of Lodz

text: Gammarus oceanicus

text: MALE

text: ERGA GOC 0307 003

text: e102c495-9794-47fa-8d04-662bba2de6bd-ERGA-specimen

characteristic: title


text: Genoscope

text: NOT PROVIDED

text: EarlhamInstitute

text: public

characteristic: Submitter Id

text: GOCE UL 1b

text: COPO

characteristic: broker name

text: University of Lodz

characteristic: collecting institution

characteristic: collection date

characteristic: collector ORCID ID

text: Germany

characteristic: geographic location (country and/or sea)

text: Boiensdorf|Wismar Bay

text: intertidal

characteristic: habitat

text: Michal Grabowski

text: University of Lodz

text: adult

characteristic: lifestage

text: Gammarus oceanicus

text: WHOLE ORGANISM

characteristic: organism part

text: ERGA

characteristic: project name

text: hand net

characteristic: sample collection method

text: Tomasz Mamos

text: University of Lodz

text: Gammarus oceanicus

text: FEMALE

text: ERGA GOC 0307 002

text: 02d865f1-29c8-427f-9b0d-ac81f73cc089-ERGA-specimen


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Genoscope

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: GOCE UL 1b

text: COPO

text: University of Lodz

text: Germany

text: Boiensdorf|Wismar Bay

text: intertidal

text: Michal Grabowski

text: University of Lodz

text: adult

text: Gammarus oceanicus

text: WHOLE ORGANISM

text: ERGA

text: hand net

text: Tomasz Mamos

characteristic: sample coordinator

characteristic: sample coordinator ORCID ID

text: University of Lodz

characteristic: sample coordinator affiliation

text: Gammarus oceanicus

text: FEMALE

characteristic: sex

text: ERGA GOC 0307 001

text: a657a852-38f7-4c02-81b5-3f3740bf3a5d-ERGA-specimen

characteristic: tolid


text: Genoscope

text: GOCE UL 8b

text: EarlhamInstitute

text: public

characteristic: Submitter Id

text: GOCE UL 8b

text: GOCE UL 8b A8

text: COPO

characteristic: broker name

text: Tomasz Mamos, Michal Grabowski, Karolina Biniek, Piotr Falczewski

text: University of Lodz

characteristic: collecting institution

characteristic: collection date

characteristic: collector ORCID ID

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Lobbe|Rugia

characteristic: geographic location (region and locality)

text: intertidal

text: Michal Grabowski

text: University of Lodz

text: adult

text: Gammarus oceanicus

characteristic: organism

text: MID BODY

text: ERGA

text: hand net

text: Tomasz Mamos

text: University of Lodz

characteristic: sample derived from

text: Gammarus oceanicus

text: MALE

text: ERGA GOC 0307 016

text: GOCE UL 8b

text: 21224a90-0d23-4893-90a1-192f6963340f-erga

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Genoscope

characteristic: GAL

text: GOCE UL 8b

text: EarlhamInstitute

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: GOCE UL 8b

text: GOCE UL 8b A8

text: COPO

text: University of Lodz

text: Germany

text: Lobbe|Rugia

text: intertidal

characteristic: habitat

text: Michal Grabowski

text: University of Lodz

text: adult

characteristic: lifestage

text: Gammarus oceanicus

text: MID BODY

characteristic: organism part

text: ERGA

characteristic: project name

text: hand net

characteristic: sample collection method

text: Tomasz Mamos

characteristic: sample coordinator

text: University of Lodz

text: Gammarus oceanicus

text: MALE

text: ERGA GOC 0307 015

text: GOCE UL 8b

text: 1f17ab53-894a-4b0c-b027-461ce465367d-erga


text: Genoscope

text: GOCE UL 7b

text: EarlhamInstitute

text: public

characteristic: Submitter Id

text: GOCE UL 7b A7

text: GOCE UL 7b

text: COPO

characteristic: broker name

text: University of Lodz

characteristic: collecting institution

characteristic: collection date

characteristic: collector ORCID ID

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Lobbe|Rugia

text: intertidal

text: Michal Grabowski

text: University of Lodz

text: adult

text: Gammarus oceanicus

text: MID BODY

text: ERGA

text: hand net

text: Tomasz Mamos

characteristic: sample coordinator ORCID ID

text: University of Lodz

characteristic: sample coordinator affiliation

text: Gammarus oceanicus

text: MALE

characteristic: sex

text: ERGA GOC 0307 014

text: GOCE UL 7b

text: 7024a65b-234b-456a-a1c8-327b10e69bec-erga

characteristic: tolid


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Genoscope

characteristic: GAL

text: GOCE UL 7b

text: EarlhamInstitute

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: GOCE UL 7b A7

text: GOCE UL 7b

text: COPO

text: University of Lodz

text: Germany

text: Lobbe|Rugia

characteristic: geographic location (region and locality)

text: intertidal

characteristic: habitat

text: Michal Grabowski

text: University of Lodz

text: adult

characteristic: lifestage

text: Gammarus oceanicus

characteristic: organism

text: MID BODY

text: ERGA

text: hand net

text: Tomasz Mamos

text: University of Lodz

characteristic: sample derived from

text: Gammarus oceanicus

text: MALE

text: ERGA GOC 0307 013

text: GOCE UL 7b

text: 41bcc000-dfc5-443e-95b4-0568d6103de9-erga

characteristic: title


text: Genoscope

text: GOCE UL 6b

text: EarlhamInstitute

text: public

characteristic: Submitter Id

text: GOCE UL 6b

text: GOCE UL 6b A6

text: COPO

characteristic: broker name

text: University of Lodz

characteristic: collecting institution

characteristic: collection date

characteristic: collector ORCID ID

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Lobbe|Rugia

text: intertidal

text: Michal Grabowski

text: University of Lodz

text: adult

text: Gammarus oceanicus

text: MID BODY

characteristic: organism part

text: ERGA

characteristic: project name

text: hand net

characteristic: sample collection method

text: Tomasz Mamos

characteristic: sample coordinator

text: University of Lodz

text: Gammarus oceanicus

text: MALE

text: ERGA GOC 0307 012

text: GOCE UL 6b

text: 72faab64-2f72-43d9-aff6-63ca68c89545-erga


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Genoscope

characteristic: GAL

text: GOCE UL 6b

text: EarlhamInstitute

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: GOCE UL 6b

text: GOCE UL 6b A6

text: COPO

text: University of Lodz

text: Germany

text: Lobbe|Rugia

characteristic: geographic location (region and locality)

text: intertidal

characteristic: habitat

text: Michal Grabowski

text: University of Lodz

text: adult

text: Gammarus oceanicus

text: MID BODY

text: ERGA

text: hand net

text: Tomasz Mamos

characteristic: sample coordinator ORCID ID

text: University of Lodz

characteristic: sample coordinator affiliation

text: Gammarus oceanicus

text: MALE

characteristic: sex

text: ERGA GOC 0307 011

text: GOCE UL 6b

text: ab4af73f-c6b0-4b18-aa1e-e4afe93cb29e-erga

characteristic: tolid


text: Genoscope

text: GOCE UL 5b

text: EarlhamInstitute

text: public

characteristic: Submitter Id

text: GOCE UL 5b

text: GOCE UL 5b A5

text: COPO

characteristic: broker name

text: University of Lodz

characteristic: collecting institution

characteristic: collection date

characteristic: collector ORCID ID

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Boiensdorf|Wismar Bay

text: intertidal

text: Michal Grabowski

text: University of Lodz

text: adult

characteristic: lifestage

text: Gammarus oceanicus

characteristic: organism

text: MID BODY

text: ERGA

text: hand net

text: Tomasz Mamos

text: University of Lodz

characteristic: sample derived from

text: Gammarus oceanicus

text: FEMALE

text: ERGA GOC 0307 010

text: GOCE UL 5b

text: 102bf041-c115-4164-9d64-58f2bdf81773-erga

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Genoscope

characteristic: GAL

text: GOCE UL 5b

text: EarlhamInstitute

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: GOCE UL 5b

text: GOCE UL 5b A5

text: COPO

text: University of Lodz

text: Germany

text: Boiensdorf|Wismar Bay

characteristic: geographic location (region and locality)

text: intertidal

characteristic: habitat

text: Michal Grabowski

text: University of Lodz

text: adult

text: Gammarus oceanicus

text: MID BODY

characteristic: organism part

text: ERGA

characteristic: project name

text: hand net

characteristic: sample collection method

text: Tomasz Mamos

characteristic: sample coordinator

text: University of Lodz

text: Gammarus oceanicus

text: FEMALE

text: ERGA GOC 0307 009

text: GOCE UL 5b

text: ee29abc5-0eb1-49f7-9165-9a678c1f2560-erga


text: Genoscope

text: GOCE UL 4b

text: EarlhamInstitute

text: public

characteristic: Submitter Id

text: GOCE UL 4b

text: GOCE UL 4b A4

text: COPO

characteristic: broker name

text: University of Lodz

characteristic: collecting institution

characteristic: collection date

characteristic: collector ORCID ID

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Boiensdorf|Wismar Bay

text: intertidal

text: Michal Grabowski

text: University of Lodz

text: adult

text: Gammarus oceanicus

text: MID BODY

text: ERGA

text: hand net

text: Tomasz Mamos

characteristic: sample coordinator ORCID ID

text: University of Lodz

characteristic: sample coordinator affiliation

text: Gammarus oceanicus

text: MALE

characteristic: sex

text: ERGA GOC 0307 008

text: GOCE UL 4b

text: d17e15c7-bc57-40c0-9eff-2bd6535f60fb-erga

characteristic: tolid


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Genoscope

characteristic: GAL

text: GOCE UL 4b

text: EarlhamInstitute

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: GOCE UL 4b

text: GOCE UL 4b A4

text: COPO

text: University of Lodz

text: Germany

text: Boiensdorf|Wismar Bay

characteristic: geographic location (region and locality)

text: intertidal

characteristic: habitat

text: Michal Grabowski

text: University of Lodz

text: adult

characteristic: lifestage

text: Gammarus oceanicus

characteristic: organism

text: MID BODY

text: ERGA

text: hand net

text: Tomasz Mamos

text: University of Lodz

characteristic: sample derived from

text: Gammarus oceanicus

text: MALE

text: ERGA GOC 0307 007

text: GOCE UL 4b

text: 78249138-922e-4d71-b4c7-bb923f943d3a-erga

characteristic: title


text: Genoscope

text: GOCE UL 3b

text: EarlhamInstitute

text: public

characteristic: Submitter Id

text: GOCE UL 3b A3

text: GOCE UL 3b

text: COPO

characteristic: broker name

text: University of Lodz

characteristic: collecting institution

characteristic: collection date

characteristic: collector ORCID ID

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Boiensdorf|Wismar Bay

text: intertidal

text: Michal Grabowski

text: University of Lodz

text: adult

text: Gammarus oceanicus

text: MID BODY

characteristic: organism part

text: ERGA

characteristic: project name

text: hand net

characteristic: sample collection method

text: Tomasz Mamos

characteristic: sample coordinator

text: University of Lodz

text: Gammarus oceanicus

text: MALE

text: ERGA GOC 0307 006

text: GOCE UL 3b

text: f8cfa39e-9abf-4e77-abc4-bd24c58a7c34-erga


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Genoscope

characteristic: GAL

text: GOCE UL 3b

text: EarlhamInstitute

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: GOCE UL 3b A3

text: GOCE UL 3b

text: COPO

text: University of Lodz

text: Germany

text: Boiensdorf|Wismar Bay

characteristic: geographic location (region and locality)

text: intertidal

characteristic: habitat

text: Michal Grabowski

text: University of Lodz

text: adult

text: Gammarus oceanicus

text: MID BODY

text: ERGA

text: hand net

text: Tomasz Mamos

characteristic: sample coordinator ORCID ID

text: University of Lodz

characteristic: sample coordinator affiliation

text: Gammarus oceanicus

text: MALE

characteristic: sex

text: ERGA GOC 0307 005

text: GOCE UL 3b

text: fe002865-58dd-4670-be7b-a0c5a756a16d-erga

characteristic: tolid


text: Genoscope

text: GOCE UL 2b

text: EarlhamInstitute

text: public

characteristic: Submitter Id

text: GOCE UL 2b

text: GOCE UL 2b A2

text: COPO

characteristic: broker name

text: University of Lodz

characteristic: collecting institution

characteristic: collection date

characteristic: collector ORCID ID

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Boiensdorf|Wismar Bay

text: intertidal

text: Michal Grabowski

text: University of Lodz

text: adult

characteristic: lifestage

text: Gammarus oceanicus

characteristic: organism

text: MID BODY

text: ERGA

text: hand net

text: Tomasz Mamos

text: University of Lodz

characteristic: sample derived from

text: Gammarus oceanicus

text: MALE

text: ERGA GOC 0307 004

text: GOCE UL 2b

text: 734ceddc-877e-46bc-9aef-c278f27bd024-erga

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Genoscope

characteristic: GAL

text: GOCE UL 2b

text: EarlhamInstitute

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: GOCE UL 2b

text: GOCE UL 2b A2

text: COPO

text: University of Lodz

text: Germany

text: Boiensdorf|Wismar Bay

characteristic: geographic location (region and locality)

text: intertidal

characteristic: habitat

text: Michal Grabowski

text: University of Lodz

text: adult

text: Gammarus oceanicus

text: MID BODY

characteristic: organism part

text: ERGA

characteristic: project name

text: hand net

characteristic: sample collection method

text: Tomasz Mamos

characteristic: sample coordinator

text: University of Lodz

text: Gammarus oceanicus

text: MALE

text: ERGA GOC 0307 003

text: GOCE UL 2b

text: 634f5b5b-fcd0-4dfa-8d38-f07edcfbabb2-erga


text: Genoscope

text: GOCE UL 1b

text: EarlhamInstitute

text: public

characteristic: Submitter Id

text: GOCE UL 1b

text: GOCE UL 1b A1

text: COPO

characteristic: broker name

text: University of Lodz

characteristic: collecting institution

characteristic: collection date

characteristic: collector ORCID ID

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Boiensdorf|Wismar Bay

text: intertidal

text: Michal Grabowski

text: University of Lodz

text: adult

text: Gammarus oceanicus

text: MID BODY

text: ERGA

text: hand net

text: Tomasz Mamos

characteristic: sample coordinator ORCID ID

text: University of Lodz

characteristic: sample coordinator affiliation

text: Gammarus oceanicus

text: FEMALE

characteristic: sex

text: ERGA GOC 0307 002

text: GOCE UL 1b

text: fb6a6f95-5404-4723-8b86-d75ef7992f1f-erga

characteristic: tolid


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Genoscope

characteristic: GAL

text: GOCE UL 1b

text: EarlhamInstitute

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: GOCE UL 1b

text: GOCE UL 1b A1

text: COPO

text: University of Lodz

text: Germany

text: Boiensdorf|Wismar Bay

characteristic: geographic location (region and locality)

text: intertidal

characteristic: habitat

text: Michal Grabowski

text: University of Lodz

text: adult

characteristic: lifestage

text: Gammarus oceanicus

characteristic: organism

text: MID BODY

text: ERGA

text: hand net

text: Tomasz Mamos

text: University of Lodz

characteristic: sample derived from

text: Gammarus oceanicus

text: FEMALE

text: ERGA GOC 0307 001

text: GOCE UL 1b

text: b9e186bc-2449-468c-a943-625b4642e666-erga

characteristic: title


text: IMBIV

text: public

characteristic: Submitter Id

characteristic: broad-scale environmental context

characteristic: collection date

unit: m

characteristic: depth

text: soil sample

characteristic: description

characteristic: elevation

text: Peanuts alongside corn as a predecessor in a soybean-corn-peanut rotation + SC6

characteristic: environmental medium

text: Argentina

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Non-till agricultural system

characteristic: local environmental context

text: soil metagenome

text: Effect of the application of PGPR microorganisms on different productive systems in Argentina

characteristic: project name

text: soil metagenome


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: IMBIV

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: soil sample

text: Peanuts alongside corn as a predecessor in a soybean-corn-peanut rotation + RI3

text: Argentina

text: Non-till agricultural system

text: soil metagenome

text: soil metagenome


text: IMBIV

text: public

characteristic: Submitter Id

text: soil sample

text: Peanuts alongside corn as a predecessor in a soybean-corn-peanut rotation + PSE10

text: Argentina

text: Non-till agricultural system

text: soil metagenome

characteristic: organism

text: soil metagenome

characteristic: title


characteristic: External Id

text: INBIONATEC

characteristic: INSDC last update

text: public

characteristic: collection date

text: strains grown in a medium with glyphosate

text: terrestial biome

characteristic: environment (biome)

text: saline pan

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: Argentina

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

text: mimarks-survey

characteristic: investigation type

text: salt metagenome

text: Screening of halotolerant glyphosate oxidases

text: salt metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: 16S rRNA amplicons from Ambargasta salt flat soil medium


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: INBIONATEC

characteristic: INSDC center name

text: public

text: control medium

characteristic: description

text: terrestial biome

text: saline pan

text: soil

text: Argentina

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

text: salt metagenome

characteristic: project name

text: salt metagenome

text: Illumina

text: soil


characteristic: External Id

text: Nanyang Technological University

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-05-04-2022-11:44:58:071-14698

characteristic: Submitter Id

text: wild type

text: human

characteristic: common name

text: RNA Nanopore sequencing of H9 rep 3: embryo (part2)

text: H9 wild type

text: DaRE lab

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Embryo

text: H9 (wild type) rep3

characteristic: title


text: public

tag: Namespace:Department of Plant Sciences,University of Cambridge

text: common toadflax

text: dorsal petal of L. vulgaris two days before flowering

text: Late

text: Linaria vulgaris

text: Linaria vulgaris

text: dorsal petal


text: Department of Plant Sciences,University of Cambridge

text: public

text: common toadflax

text: ventral petal of L. vulgaris two days before flowering

text: Late

text: Linaria vulgaris

text: Linaria vulgaris

text: ventral petal (including spur)


text: public

text: common toadflax

text: dorsal petal of L. vulgaris four and five days before flowering

text: intermediate

text: Linaria vulgaris

text: Linaria vulgaris

text: dorsal petal


text: public

text: common toadflax

text: ventral petal of L. vulgaris four and five days before flowering

text: intermediate

text: Linaria vulgaris

text: Linaria vulgaris

text: ventral petal (including spur)


text: public

text: common toadflax

text: dorsal petal of L. vulgaris eight or nine days before flowering

text: early

text: Linaria vulgaris

text: Linaria vulgaris

text: dorsal petal


text: public

text: common toadflax

text: ventral petal of L. vulgaris eight or nine days before flowering

text: early

text: Linaria vulgaris

text: Linaria vulgaris

text: ventral petal (including spur)


text: public

text: snapdragon

text: dorsal petal of A. majus two days before flowering

text: Late

text: Antirrhinum majus

text: Antirrhinum majus

text: dorsal petal


text: public

text: snapdragon

text: ventral petal of A. majus two days before flowering

text: Late

text: Antirrhinum majus

text: Antirrhinum majus

text: ventral petal (including gibba)


text: public

text: snapdragon

text: dorsal petal of A. majus four and five days before flowering

text: intermediate

text: Antirrhinum majus

text: Antirrhinum majus

text: dorsal petal


text: public

text: snapdragon

text: ventral petal of A. majus four and five days before flowering

text: intermediate

text: Antirrhinum majus

text: Antirrhinum majus

text: ventral petal (including gibba)


text: public

text: snapdragon

text: dorsal petal of A. majus eight or nine days before flowering

text: early

text: Antirrhinum majus

text: Antirrhinum majus

text: dorsal petal


text: public

text: snapdragon

text: ventral petal of A. majus eight or nine days before flowering

text: early

text: Antirrhinum majus

text: Antirrhinum majus

text: ventral petal (including gibba)


text: public

tag: Namespace:CENTER FOR BIOTECHNOLOGY, BIELEFELD UNIVERSITY

text: wine grape

text: RNA-Seq reads of 6 hpi control Sample2

text: GEILWEILERHOF 2014-099-0003

characteristic: genotype

text: 0 m

text: Germany

text: Vitis vinifera

text: Leaf

text: Vitis vinifera

text: weisshaa@cebitec.uni-bielefeld.de

text: Bernd Weisshaar

text: RNA-Seq 6hpi control Sample2

text: Green house environment condition CO_715:0000190


characteristic: ENA first public

text: CENTER FOR BIOTECHNOLOGY, BIELEFELD UNIVERSITY

text: public

text: wine grape

text: RNA-Seq reads of 6 hpi control Sample3

text: GEILWEILERHOF 2014-099-0003

text: 0 m

text: Germany

text: Vitis vinifera

text: Leaf

text: Vitis vinifera

text: weisshaa@cebitec.uni-bielefeld.de

text: Bernd Weisshaar

text: RNA-Seq 6hpi control Sample3

text: Green house environment condition CO_715:0000191


characteristic: ENA-CHECKLIST

text: public

text: wine grape

text: RNA-Seq reads of 12 hpi Sample1

text: GEILWEILERHOF 2014-099-0003

text: 0 m

text: Germany

text: Vitis vinifera

text: Leaf

text: Vitis vinifera

text: weisshaa@cebitec.uni-bielefeld.de

text: Bernd Weisshaar

text: RNA-Seq 12hpi Sample1

text: Green house environment condition CO_715:0000192


characteristic: INSDC center name

text: public

text: wine grape

text: RNA-Seq reads of 12 hpi Sample2

text: GEILWEILERHOF 2014-099-0003

text: 0 m

text: Germany

text: Vitis vinifera

text: Leaf

text: Vitis vinifera

text: weisshaa@cebitec.uni-bielefeld.de

text: Bernd Weisshaar

text: RNA-Seq 12hpi Sample2

text: Green house environment condition CO_715:0000193


text: public

text: wine grape

text: RNA-Seq reads of 12 hpi Sample3

characteristic: description

text: GEILWEILERHOF 2014-099-0003

text: 0 m

text: Germany

text: Vitis vinifera

text: Leaf

text: Vitis vinifera

text: weisshaa@cebitec.uni-bielefeld.de

text: Bernd Weisshaar

text: RNA-Seq 12hpi Sample3

text: Green house environment condition CO_715:0000194


text: public

text: wine grape

text: RNA-Seq reads of 12 hpi control Sample1

text: GEILWEILERHOF 2014-099-0003

text: 0 m

text: Germany

text: Vitis vinifera

text: Leaf

text: Vitis vinifera

text: weisshaa@cebitec.uni-bielefeld.de

text: Bernd Weisshaar

text: RNA-Seq 12hpi control Sample1

text: Green house environment condition CO_715:0000195


text: public

text: wine grape

text: RNA-Seq reads of 12 hpi control Sample2

text: GEILWEILERHOF 2014-099-0003

text: 0 m

text: Germany

text: Vitis vinifera

text: Leaf

text: Vitis vinifera

text: weisshaa@cebitec.uni-bielefeld.de

text: Bernd Weisshaar

text: RNA-Seq 12hpi control Sample2

text: Green house environment condition CO_715:0000196


text: public

text: wine grape

text: RNA-Seq reads of 12 hpi control Sample3

text: GEILWEILERHOF 2014-099-0003

text: 0 m

text: Germany

text: Vitis vinifera

text: Leaf

text: Vitis vinifera

text: weisshaa@cebitec.uni-bielefeld.de

text: Bernd Weisshaar

text: RNA-Seq 12hpi control Sample3

text: Green house environment condition CO_715:0000197


text: public

text: wine grape

text: RNA-Seq reads of 24 hpi Sample1

text: GEILWEILERHOF 2014-099-0003

text: 0 m

text: Germany

text: Vitis vinifera

text: Leaf

text: Vitis vinifera

text: weisshaa@cebitec.uni-bielefeld.de

text: Bernd Weisshaar

text: RNA-Seq 24hpi Sample1

text: Green house environment condition CO_715:0000198


text: public

text: wine grape

text: RNA-Seq reads of 24 hpi Sample2

text: GEILWEILERHOF 2014-099-0003

text: 0 m

text: Germany

text: Vitis vinifera

text: Leaf

text: Vitis vinifera

text: weisshaa@cebitec.uni-bielefeld.de

text: Bernd Weisshaar

text: RNA-Seq 24hpi Sample2

text: Green house environment condition CO_715:0000199


text: public

text: wine grape

text: RNA-Seq reads of 24 hpi Sample3

text: GEILWEILERHOF 2014-099-0003

text: 0 m

text: Germany

text: Vitis vinifera

text: Leaf

text: Vitis vinifera

text: weisshaa@cebitec.uni-bielefeld.de

text: Bernd Weisshaar

text: RNA-Seq 24hpi Sample3

text: Green house environment condition CO_715:0000200


text: public

text: wine grape

text: RNA-Seq reads of 24 hpi control Sample1

text: GEILWEILERHOF 2014-099-0003

text: 0 m

text: Germany

text: Vitis vinifera

text: Leaf

text: Vitis vinifera

text: weisshaa@cebitec.uni-bielefeld.de

text: Bernd Weisshaar

text: RNA-Seq 24hpi control Sample1

text: Green house environment condition CO_715:0000201


text: public

text: wine grape

text: RNA-Seq reads of 24 hpi control Sample2

text: GEILWEILERHOF 2014-099-0003

text: 0 m

text: Germany

text: Vitis vinifera

text: Leaf

text: Vitis vinifera

text: weisshaa@cebitec.uni-bielefeld.de

text: Bernd Weisshaar

text: RNA-Seq 24hpi control Sample2

text: Green house environment condition CO_715:0000202


text: JIC

text: public

text: thale cress

text: Purified genomic DNA from Arabidopsis leaves are tagmented with Tn5 from an Illumina Nextera kit. Replicate: 1st biological replicate. Library was amplified with custom-design and 9nt-space dual-index primers. After multiplexed with other samples are sequenced with four flow cells of Nextseq 500/550 high output kit (75 cycles), and demultiplexed with Illumina dcl2fastq.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: Purified genomic DNA from Arabidopsis leaves are tagmented with Tn5 from an Illumina Nextera kit. Replicate: 2nd biological replicate. Library was amplified with custom-design and 9nt-space dual-index primers. After multiplexed with other samples are sequenced with four flow cells of Nextseq 500/550 high output kit (75 cycles), and demultiplexed with Illumina dcl2fastq.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: 22 degree, short-day light, 75% humidity. Genotype: gh=sard1 cbp60g double mutant. Replicate: 1st biological replicate. Treatment: Infiltration leaves with OD600=0.2 of Pf0-1:AvrRps4 (wild type) in 10mM MgCl2. Sampling: 4 hours post infiltration. Fresh nuclei were extracted from mature leaves from plants which are 5-week old and sorted with flow cytometry (fluorescence-activated nuclei sorting), and tagmented with Tn5 from an Illumina Nextera kit. ATACseq library was amplified with custom-design and 9nt-space dual-index primers. After multiplexed with other samples are sequenced with four flow cells of Nextseq 500/550 high output kit (75 cycles), and demultiplexed with Illumina dcl2fastq.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: 22 degree, short-day light, 75% humidity. Genotype: gh=sard1 cbp60g double mutant. Replicate: 1st biological replicate. Treatment: Infiltration leaves with OD600=0.2 of Pf0-1:AvrRps4(KRVY135-138AAAA mutant) in 10mM MgCl2. Sampling: 4 hours post infiltration. Fresh nuclei were extracted from mature leaves from plants which are 5-week old and sorted with flow cytometry (fluorescence-activated nuclei sorting), and tagmented with Tn5 from an Illumina Nextera kit. ATACseq library was amplified with custom-design and 9nt-space dual-index primers. After multiplexed with other samples are sequenced with four flow cells of Nextseq 500/550 high output kit (75 cycles), and demultiplexed with Illumina dcl2fastq.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: 22 degree, short-day light, 75% humidity. Genotype: gh=sard1 cbp60g double mutant. Replicate: 2nd biological replicate. Treatment: Infiltration leaves with OD600=0.2 of Pf0-1:AvrRps4(KRVY135-138AAAA mutant) in 10mM MgCl2. Sampling: 4 hours post infiltration. Fresh nuclei were extracted from mature leaves from plants which are 5-week old and sorted with flow cytometry (fluorescence-activated nuclei sorting), and tagmented with Tn5 from an Illumina Nextera kit. ATACseq library was amplified with custom-design and 9nt-space dual-index primers. After multiplexed with other samples are sequenced with four flow cells of Nextseq 500/550 high output kit (75 cycles), and demultiplexed with Illumina dcl2fastq.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: 22 degree, short-day light, 75% humidity. Genotype: gh=sard1 cbp60g double mutant. Replicate: 1st biological replicate. Treatment: Infiltration leaves with 10mM MgCl2. Sampling: 4 hours post infiltration. Fresh nuclei were extracted from mature leaves from plants which are 5-week old and sorted with flow cytometry (fluorescence-activated nuclei sorting), and tagmented with Tn5 from an Illumina Nextera kit. ATACseq library was amplified with custom-design and 9nt-space dual-index primers. After multiplexed with other samples are sequenced with four flow cells of Nextseq 500/550 high output kit (75 cycles), and demultiplexed with Illumina dcl2fastq.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: 22 degree, short-day light, 75% humidity. Genotype: gh=sard1 cbp60g double mutant. Replicate: 1st biological replicate. Treatment: untreated. Fresh nuclei were extracted from mature leaves from plants which are 5-week old and sorted with flow cytometry (fluorescence-activated nuclei sorting), and tagmented with Tn5 from an Illumina Nextera kit. ATACseq library was amplified with custom-design and 9nt-space dual-index primers. After multiplexed with other samples are sequenced with four flow cells of Nextseq 500/550 high output kit (75 cycles), and demultiplexed with Illumina dcl2fastq.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: 22 degree, short-day light, 75% humidity. Genotype: setigh= SETI with wild-type inducible AvrRps4 (Ngou et al 2019 JXB) crossed into sard1 cbp60g double mutant. Treatment: e2=beta-estradiol, infiltrated with 50uM e2 (in water), harvested at 3.5 hours post infiltration (hpi). Replicate: 1st biological replicate (1/9). Fresh nuclei were extracted from mature leaves from plants which are 5-week old and sorted with flow cytometry (fluorescence-activated nuclei sorting), and tagmented with Tn5 from an Illumina Nextera kit. ATACseq library was amplified with custom-design and 9nt-space dual-index primers. After multiplexed with other samples are sequenced with four flow cells of Nextseq 500/550 high output kit (75 cycles), and demultiplexed with Illumina dcl2fastq.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: 22 degree, short-day light, 75% humidity. Genotype: setigh= SETI with wild-type inducible AvrRps4 (Ngou et al 2019 JXB) crossed into sard1 cbp60g double mutant. Treatment: mk=mock treatment, infiltrated with 0.1% DMSO (in water), harvested at 3.5 hours post infiltration (hpi). Replicate: 1st biological replicate (1/9). Fresh nuclei were extracted from mature leaves from plants which are 5-week old and sorted with flow cytometry (fluorescence-activated nuclei sorting), and tagmented with Tn5 from an Illumina Nextera kit. ATACseq library was amplified with custom-design and 9nt-space dual-index primers. After multiplexed with other samples are sequenced with four flow cells of Nextseq 500/550 high output kit (75 cycles), and demultiplexed with Illumina dcl2fastq.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: 22 degree, short-day light, 75% humidity. Genotype: setigh= SETI with wild-type inducible AvrRps4 (Ngou et al 2019 JXB) crossed into sard1 cbp60g double mutant. Treatment: un=no treatment/untreated. Replicate: 1st biological replicate (1/9). Fresh nuclei were extracted from mature leaves from plants which are 5-week old and sorted with flow cytometry (fluorescence-activated nuclei sorting), and tagmented with Tn5 from an Illumina Nextera kit. ATACseq library was amplified with custom-design and 9nt-space dual-index primers. After multiplexed with other samples are sequenced with four flow cells of Nextseq 500/550 high output kit (75 cycles), and demultiplexed with Illumina dcl2fastq.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: 22 degree, short-day light, 75% humidity. Genotype: setiwt= setikv= SETI with inducible mutant AvrRps4 (KRVY135-138AAAA) (Ngou et al 2019 JXB). Treatment: e2=beta-estradiol, infiltrated with 50uM e2 (in water), harvested at 3.5 hours post infiltration (hpi). Replicate: 1st biological replicate (1/9). Fresh nuclei were extracted from mature leaves from plants which are 5-week old and sorted with flow cytometry (fluorescence-activated nuclei sorting), and tagmented with Tn5 from an Illumina Nextera kit. ATACseq library was amplified with custom-design and 9nt-space dual-index primers. After multiplexed with other samples are sequenced with four flow cells of Nextseq 500/550 high output kit (75 cycles), and demultiplexed with Illumina dcl2fastq.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: 22 degree, short-day light, 75% humidity. Genotype: setikv= SETI with inducible mutant AvrRps4 (KRVY135-138AAAA) (Ngou et al 2019 JXB). Treatment: mk=mock treatment, infiltrated with 0.1% DMSO (in water), harvested at 3.5 hours post infiltration (hpi). Replicate: 1st biological replicate (1/9). Fresh nuclei were extracted from mature leaves from plants which are 5-week old and sorted with flow cytometry (fluorescence-activated nuclei sorting), and tagmented with Tn5 from an Illumina Nextera kit. ATACseq library was amplified with custom-design and 9nt-space dual-index primers. After multiplexed with other samples are sequenced with four flow cells of Nextseq 500/550 high output kit (75 cycles), and demultiplexed with Illumina dcl2fastq.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: 22 degree, short-day light, 75% humidity. Genotype: setikv= SETI with inducible mutant AvrRps4 (KRVY135-138AAAA) (Ngou et al 2019 JXB). Treatment: un=no treatment/untreated. Replicate: 1st biological replicate (1/9). Fresh nuclei were extracted from mature leaves from plants which are 5-week old and sorted with flow cytometry (fluorescence-activated nuclei sorting), and tagmented with Tn5 from an Illumina Nextera kit. ATACseq library was amplified with custom-design and 9nt-space dual-index primers. After multiplexed with other samples are sequenced with four flow cells of Nextseq 500/550 high output kit (75 cycles), and demultiplexed with Illumina dcl2fastq.

text: Arabidopsis thaliana

text: Arabidopsis thaliana