text: public

text: E-MTAB-13664_4:D33_Epi_all_SLX-19902-20446_SIGAG7

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Epithelial enriched - unsorted

characteristic: fraction

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

characteristic: organism part

characteristic: parity

text: 10x 3' v3

characteristic: protocol

text: Homo sapiens

text: female

text: prophylactic mastectomy


status: PUBLIC

characteristic: ENA first public

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13664_4:D21_Epi_all_SLX-20005-20446_SIGAA6

tag: Namespace:Cambridge Stem Cell Institute Department of Pharmacology

characteristic: Submitter Id

unit: year

characteristic: age

characteristic: batch

text: ArrayExpress

text: not collected

text: human

text: Protocols: All frozen primary human breast tissue was obtained from the Breast Cancer Now Tissue bank (REC 15/EE/0192). Prior to freezing, fresh tissues were minced and digested O/N in 1 mg/mL Collagenase 1A and 1 mg/mL Hyaluronidase in an orbital shaker, washed 2-3 times and sedimented to allow for the separation between epithelial-enriched and stromal fractions, which were then separately cryostored. Frozen vials of epithelial-enriched or stromal-enriched fractions were defrosted, washed in PBS and centrifuged at 400 g for 5 minutes and resuspended in 2 mL of freshly prepared PBS + 0.025% Trypsin and 0.4 mg/mL Deoxyribonuclease 1 (DNase). Samples were then incubated at 37°C with pipetting up and down for 2-3 minutes until smoothly digested or up to a maximum of 10 minutes. Next, samples were washed and centrifuged for 20 minutes at 400 g with slow break. The pellet was resuspended in media + DNase until homogeneous, then diluted and filtered through a 40 μm cell strainer. After centrifugation for 5 minutes at 400 g, the pellet was resuspended by pipetting up and down in 200 μL of Cell Preparation Medium + 10 μL of 10 mg/mL DNase until homogeneous, then washed in 3-6 mL of media. 30,000 cells were resuspended into 48 μL of media and 400 Human mammary epithelial cells (HuMECs, Thermo Fisher Scientific A10565) were added as spike-in, and samples were submitted for scRNA-seq (either stromal-enriched or epithelial-enriched samples). For the epithelial-enriched fraction only, the rest of the processed sample was stained with primary antibodies: CD45-APC, CD31-APC, EPCAM-AF488, CD49f-PE/Cy7. DAPI was used to detect dead cells. Cells were filtered through a cell strainer before sorting. Sorting of cells was done using a FACS Aria Fusion sorter. Single-stained control cells were used to perform compensation manually and unstained cells were used to set gates. After doublets, dead cells and contaminating haematopoietic and endothelial cells (referred to as lineage) were gated, up to 30,000 LASPs were sorted for scRNA-seq (with the addition of 400 HuMECs as spike-in). Approximately 30,000 mammary cells per sample were loaded into separate channels of a single Chromium controller (10x Genomics) chip. Consequently, gel bead-in-emulsions (GEMS) were generated using 10X Genomics Single Cell 3’ Reagent Kit v3. Sequencing libraries were prepared using Single Cell 3’ Reagent Kits v3 (10X Genomics) and then converted using the NovaSeq 6000 S2 Reagent Kit (Illumina).

text: adult

text: normal

text: Epithelial enriched - unsorted

text: not collected

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: breast

text: 10x 3' v3

characteristic: sample id

text: Homo sapiens

text: female

characteristic: sex

text: reduction mammoplasty

characteristic: surgery type

characteristic: title


text: Cambridge Stem Cell Institute Department of Pharmacology

text: public

text: E-MTAB-13664_4:D13_Epi_all_SLX-19902_SIGAD4

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Epithelial enriched - unsorted

characteristic: fraction

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

characteristic: organism part

characteristic: parity

text: 10x 3' v3

characteristic: protocol

text: Homo sapiens

text: female

text: reduction mammoplasty


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13664_4:D41_Str_all_SLX-19864-20262_SIGAE8

characteristic: Submitter Id

characteristic: age

characteristic: batch

text: ArrayExpress

text: not collected

text: human

text: adult

text: normal

text: Stroma enriched - unsorted

text: not collected

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: breast

text: 10x 3' v3

characteristic: sample id

text: Homo sapiens

text: female

text: prophylactic mastectomy


text: public

text: E-MTAB-13664_4:D43_Epi_LASP_SLX-20005_SIGAB9

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Epithelial enriched - LASP-sorted

characteristic: fraction

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

text: 10x 3' v3

text: Homo sapiens

text: female

characteristic: sex

text: prophylactic mastectomy

characteristic: surgery type

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13664_4:D22_Str_all_SLX-19864_SIGAF5

characteristic: Submitter Id

characteristic: age

characteristic: batch

text: ArrayExpress

text: not collected

text: human

text: adult

text: normal

text: Stroma enriched - unsorted

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

characteristic: organism part

characteristic: parity

text: 10x 3' v3

characteristic: protocol

text: Homo sapiens

text: female

text: reduction mammoplasty


text: public

text: E-MTAB-13664_4:D5_Epi_all_SLX-20261_SIGAG2

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Epithelial enriched - unsorted

characteristic: fraction

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: breast

text: unknown

text: 10x 3' v3

characteristic: sample id

text: Homo sapiens

text: female

text: reduction mammoplasty


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13664_4:D38_Epi_all_SLX-20261_SIGAA3

characteristic: Submitter Id

characteristic: age

characteristic: batch

text: ArrayExpress

text: not collected

text: human

text: adult

text: normal

text: Epithelial enriched - unsorted

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

text: 10x 3' v3

text: Homo sapiens

text: female

characteristic: sex

text: prophylactic mastectomy

characteristic: surgery type

characteristic: title


text: public

text: E-MTAB-13664_4:D40_Epi_all_SLX-19864-20262_SIGAC6

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Epithelial enriched - unsorted

characteristic: fraction

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

characteristic: organism part

characteristic: parity

text: 10x 3' v3

characteristic: protocol

text: Homo sapiens

text: female

text: prophylactic mastectomy


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13664_4:D8_Epi_all_SLX-19864-20262_SIGAG4

characteristic: Submitter Id

characteristic: age

characteristic: batch

text: ArrayExpress

text: not collected

text: human

text: adult

text: normal

text: Epithelial enriched - unsorted

text: not collected

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: breast

text: 10x 3' v3

characteristic: sample id

text: Homo sapiens

text: female

text: reduction mammoplasty


text: public

text: E-MTAB-13664_4:D41_Epi_LASP_SLX-19864_SIGAB6

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Epithelial enriched - LASP-sorted

characteristic: fraction

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

text: 10x 3' v3

text: Homo sapiens

text: female

characteristic: sex

text: prophylactic mastectomy

characteristic: surgery type

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13664_4:D23_Str_all_SLX-20005-20446_SIGAD12

characteristic: Submitter Id

characteristic: age

characteristic: batch

text: ArrayExpress

text: not collected

text: human

text: adult

text: normal

text: Stroma enriched - unsorted

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

characteristic: organism part

characteristic: parity

text: 10x 3' v3

characteristic: protocol

text: Homo sapiens

text: female

text: prophylactic mastectomy


text: public

text: E-MTAB-13664_4:D25_Epi_all_SLX-19864_SIGAC5

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Epithelial enriched - unsorted

characteristic: fraction

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: breast

text: 10x 3' v3

characteristic: sample id

text: Homo sapiens

text: female

text: prophylactic mastectomy


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13664_4:D54_Str_all_SLX-19902-20449_SIGAA3

characteristic: Submitter Id

characteristic: age

characteristic: batch

text: ArrayExpress

text: not collected

text: human

text: adult

text: normal

text: Stroma enriched - unsorted

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

text: 10x 3' v3

text: Homo sapiens

text: female

characteristic: sex

text: contralateral

characteristic: surgery type

characteristic: title


text: public

text: E-MTAB-13664_4:D50_Epi_all_SLX-19864-20262_SIGAA5

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Epithelial enriched - unsorted

characteristic: fraction

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

characteristic: organism part

characteristic: parity

text: 10x 3' v3

characteristic: protocol

text: Homo sapiens

text: female

text: contralateral


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13664_4:D17_Epi_all_SLX-19864_SIGAC9

characteristic: Submitter Id

characteristic: age

characteristic: batch

text: ArrayExpress

text: not collected

text: human

text: adult

text: normal

text: Epithelial enriched - unsorted

text: not collected

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: breast

text: 10x 3' v3

characteristic: sample id

text: Homo sapiens

text: female

text: reduction mammoplasty


text: public

text: E-MTAB-13664_4:D34_Str_all_SLX-20261-20448_SIGAC6

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Stroma enriched - unsorted

characteristic: fraction

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

text: 10x 3' v3

text: Homo sapiens

text: female

characteristic: sex

text: prophylactic mastectomy

characteristic: surgery type

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13664_4:D16_Str_all_SLX-19864_SIGAH6

characteristic: Submitter Id

characteristic: age

characteristic: batch

text: ArrayExpress

text: not collected

text: human

text: Protocols: All frozen primary human breast tissue was obtained from the Breast Cancer Now Tissue bank (REC 15/EE/0192). Prior to freezing, fresh tissues were minced and digested O/N in 1 mg/mL Collagenase 1A and 1 mg/mL Hyaluronidase in an orbital shaker, washed 2-3 times and sedimented to allow for the separation between epithelial-enriched and stromal fractions, which were then separately cryostored. Frozen vials of epithelial-enriched or stromal-enriched fractions were defrosted, washed in PBS and centrifuged at 400 g for 5 minutes and resuspended in 2 mL of freshly prepared PBS + 0.025% Trypsin and 0.4 mg/mL Deoxyribonuclease 1 (DNase). Samples were then incubated at 37°C with pipetting up and down for 2-3 minutes until smoothly digested or up to a maximum of 10 minutes. Next, samples were washed and centrifuged for 20 minutes at 400 g with slow break. The pellet was resuspended in media + DNase until homogeneous, then diluted and filtered through a 40 μm cell strainer. After centrifugation for 5 minutes at 400 g, the pellet was resuspended by pipetting up and down in 200 μL of Cell Preparation Medium + 10 μL of 10 mg/mL DNase until homogeneous, then washed in 3-6 mL of media. 30,000 cells were resuspended into 48 μL of media and 400 Human mammary epithelial cells (HuMECs, Thermo Fisher Scientific A10565) were added as spike-in, and samples were submitted for scRNA-seq (either stromal-enriched or epithelial-enriched samples). For the epithelial-enriched fraction only, the rest of the processed sample was stained with primary antibodies: CD45-APC, CD31-APC, EPCAM-AF488, CD49f-PE/Cy7. DAPI was used to detect dead cells. Cells were filtered through a cell strainer before sorting. Sorting of cells was done using a FACS Aria Fusion sorter. Single-stained control cells were used to perform compensation manually and unstained cells were used to set gates. After doublets, dead cells and contaminating haematopoietic and endothelial cells (referred to as lineage) were gated, up to 30,000 LASPs were sorted for scRNA-seq (with the addition of 400 HuMECs as spike-in). Approximately 30,000 mammary cells per sample were loaded into separate channels of a single Chromium controller (10x Genomics) chip. Consequently, gel bead-in-emulsions (GEMS) were generated using 10X Genomics Single Cell 3’ Reagent Kit v3. Sequencing libraries were prepared using Single Cell 3’ Reagent Kits v3 (10X Genomics) and then converted using the NovaSeq 6000 S2 Reagent Kit (Illumina).

text: adult

text: normal

text: Stroma enriched - unsorted

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

characteristic: organism part

characteristic: parity

text: 10x 3' v3

characteristic: protocol

text: Homo sapiens

text: female

text: reduction mammoplasty


text: Cambridge Stem Cell Institute Department of Pharmacology

text: public

text: E-MTAB-13664_4:D10_Epi_all_SLX-20261_SIGAE2

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Epithelial enriched - unsorted

characteristic: fraction

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: breast

text: 10x 3' v3

characteristic: sample id

text: Homo sapiens

text: female

text: reduction mammoplasty


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13664_4:D55_Str_all_SLX-20005-20446_SIGAC12

characteristic: Submitter Id

characteristic: age

characteristic: batch

text: ArrayExpress

text: not collected

text: human

text: adult

text: normal

text: Stroma enriched - unsorted

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

text: 10x 3' v3

text: Homo sapiens

text: female

characteristic: sex

text: contralateral

characteristic: surgery type

characteristic: title


text: public

text: E-MTAB-13664_4:D12_Str_all_SLX-19864-20262_SIGAF6

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Stroma enriched - unsorted

characteristic: fraction

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

characteristic: organism part

characteristic: parity

text: 10x 3' v3

characteristic: protocol

text: Homo sapiens

text: female

text: reduction mammoplasty


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13664_4:D7_Str_all_SLX-20005-20446_SIGAD7

characteristic: Submitter Id

characteristic: age

characteristic: batch

text: ArrayExpress

text: not collected

text: human

text: adult

text: normal

text: Stroma enriched - unsorted

text: not collected

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: breast

text: 10x 3' v3

characteristic: sample id

text: Homo sapiens

text: female

text: reduction mammoplasty


text: public

text: E-MTAB-13664_4:D55_Epi_all_SLX-19864_SIGAA8

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Epithelial enriched - unsorted

characteristic: fraction

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

text: 10x 3' v3

text: Homo sapiens

text: female

characteristic: sex

text: contralateral

characteristic: surgery type

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13664_4:D26_Str_all_SLX-20005-20446_SIGAE11

characteristic: Submitter Id

characteristic: age

characteristic: batch

text: ArrayExpress

text: not collected

text: human

text: adult

text: normal

text: Stroma enriched - unsorted

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

characteristic: organism part

characteristic: parity

text: 10x 3' v3

characteristic: protocol

text: Homo sapiens

text: female

text: prophylactic mastectomy


text: public

text: E-MTAB-13664_4:D51_Str_all_SLX-20870_SIGAC9

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Stroma enriched - unsorted

characteristic: fraction

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: breast

text: 10x 3' v3

characteristic: sample id

text: Homo sapiens

text: female

text: contralateral


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13664_4:D3_Epi_all_SLX-20005_SIGAG3

characteristic: Submitter Id

characteristic: age

characteristic: batch

text: ArrayExpress

text: not collected

text: human

text: adult

text: normal

text: Epithelial enriched - unsorted

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

text: unknown

text: 10x 3' v3

text: Homo sapiens

text: female

characteristic: sex

text: reduction mammoplasty

characteristic: surgery type

characteristic: title


text: public

text: E-MTAB-13664_4:D38_Str_all_SLX-19864-20262_SIGAG6

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Stroma enriched - unsorted

characteristic: fraction

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

characteristic: organism part

characteristic: parity

text: 10x 3' v3

characteristic: protocol

text: Homo sapiens

text: female

text: prophylactic mastectomy


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13664_4:D4_Str_all_SLX-19864_SIGAD8

characteristic: Submitter Id

characteristic: age

characteristic: batch

text: ArrayExpress

text: not collected

text: human

text: adult

text: normal

text: Stroma enriched - unsorted

text: not collected

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: breast

text: 10x 3' v3

characteristic: sample id

text: Homo sapiens

text: female

text: reduction mammoplasty


text: public

text: E-MTAB-13664_4:failed_1_Epi_all_SLX-20261_SIGAE3

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Epithelial enriched - unsorted

characteristic: fraction

text: unknown

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

text: 10x 3' v3

text: Homo sapiens

text: female

characteristic: sex

text: prophylactic mastectomy

characteristic: surgery type

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13664_4:D30_Str_all_SLX-19864-20262_SIGAE5

characteristic: Submitter Id

characteristic: age

characteristic: batch

text: ArrayExpress

text: not collected

text: human

text: adult

text: normal

text: Stroma enriched - unsorted

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

characteristic: organism part

characteristic: parity

text: 10x 3' v3

characteristic: protocol

text: Homo sapiens

text: female

text: prophylactic mastectomy


text: public

text: E-MTAB-13664_4:D4_Epi_LASP_SLX-19864_SIGAE7

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Epithelial enriched - LASP-sorted

characteristic: fraction

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: breast

text: 10x 3' v3

characteristic: sample id

text: Homo sapiens

text: female

text: reduction mammoplasty


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13664_4:D11_Epi_LASP_SLX-20005_SIGAF9

characteristic: Submitter Id

characteristic: age

characteristic: batch

text: ArrayExpress

text: not collected

text: human

text: adult

text: normal

text: Epithelial enriched - LASP-sorted

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

text: 10x 3' v3

text: Homo sapiens

text: female

characteristic: sex

text: reduction mammoplasty

characteristic: surgery type

characteristic: title


text: public

text: E-MTAB-13664_4:D6_Str_all_SLX-19864-20262_SIGAE6

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Stroma enriched - unsorted

characteristic: fraction

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

characteristic: organism part

characteristic: parity

text: 10x 3' v3

characteristic: protocol

text: Homo sapiens

text: female

text: reduction mammoplasty


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13664_4:D48_Epi_all_SLX-20005_SIGAF6

characteristic: Submitter Id

characteristic: age

characteristic: batch

text: ArrayExpress

text: not collected

text: human

text: Protocols: All frozen primary human breast tissue was obtained from the Breast Cancer Now Tissue bank (REC 15/EE/0192). Prior to freezing, fresh tissues were minced and digested O/N in 1 mg/mL Collagenase 1A and 1 mg/mL Hyaluronidase in an orbital shaker, washed 2-3 times and sedimented to allow for the separation between epithelial-enriched and stromal fractions, which were then separately cryostored. Frozen vials of epithelial-enriched or stromal-enriched fractions were defrosted, washed in PBS and centrifuged at 400 g for 5 minutes and resuspended in 2 mL of freshly prepared PBS + 0.025% Trypsin and 0.4 mg/mL Deoxyribonuclease 1 (DNase). Samples were then incubated at 37°C with pipetting up and down for 2-3 minutes until smoothly digested or up to a maximum of 10 minutes. Next, samples were washed and centrifuged for 20 minutes at 400 g with slow break. The pellet was resuspended in media + DNase until homogeneous, then diluted and filtered through a 40 μm cell strainer. After centrifugation for 5 minutes at 400 g, the pellet was resuspended by pipetting up and down in 200 μL of Cell Preparation Medium + 10 μL of 10 mg/mL DNase until homogeneous, then washed in 3-6 mL of media. 30,000 cells were resuspended into 48 μL of media and 400 Human mammary epithelial cells (HuMECs, Thermo Fisher Scientific A10565) were added as spike-in, and samples were submitted for scRNA-seq (either stromal-enriched or epithelial-enriched samples). For the epithelial-enriched fraction only, the rest of the processed sample was stained with primary antibodies: CD45-APC, CD31-APC, EPCAM-AF488, CD49f-PE/Cy7. DAPI was used to detect dead cells. Cells were filtered through a cell strainer before sorting. Sorting of cells was done using a FACS Aria Fusion sorter. Single-stained control cells were used to perform compensation manually and unstained cells were used to set gates. After doublets, dead cells and contaminating haematopoietic and endothelial cells (referred to as lineage) were gated, up to 30,000 LASPs were sorted for scRNA-seq (with the addition of 400 HuMECs as spike-in). Approximately 30,000 mammary cells per sample were loaded into separate channels of a single Chromium controller (10x Genomics) chip. Consequently, gel bead-in-emulsions (GEMS) were generated using 10X Genomics Single Cell 3’ Reagent Kit v3. Sequencing libraries were prepared using Single Cell 3’ Reagent Kits v3 (10X Genomics) and then converted using the NovaSeq 6000 S2 Reagent Kit (Illumina).

text: adult

text: normal

text: Epithelial enriched - unsorted

text: unknown

text: not collected

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: breast

text: 10x 3' v3

characteristic: sample id

text: Homo sapiens

text: female

text: prophylactic mastectomy


text: Cambridge Stem Cell Institute Department of Pharmacology

text: public

text: E-MTAB-13664_4:D38_Epi_LASP_SLX-20261-20448_SIGAB3

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Epithelial enriched - LASP-sorted

characteristic: fraction

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

text: 10x 3' v3

text: Homo sapiens

text: female

characteristic: sex

text: prophylactic mastectomy

characteristic: surgery type

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13664_4:D27_Epi_all_SLX-19864_SIGAA9

characteristic: Submitter Id

characteristic: age

characteristic: batch

text: ArrayExpress

text: not collected

text: human

text: adult

text: normal

text: Epithelial enriched - unsorted

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

characteristic: organism part

characteristic: parity

text: 10x 3' v3

characteristic: protocol

text: Homo sapiens

text: female

text: prophylactic mastectomy


text: public

text: E-MTAB-13664_4:D7_Epi_all_SLX-20005_SIGAC11

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Epithelial enriched - unsorted

characteristic: fraction

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: breast

text: 10x 3' v3

characteristic: sample id

text: Homo sapiens

text: female

text: reduction mammoplasty


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13664_4:D9_Epi_all_SLX-20261_SIGAC10

characteristic: Submitter Id

characteristic: age

characteristic: batch

text: ArrayExpress

text: not collected

text: human

text: adult

text: normal

text: Epithelial enriched - unsorted

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

text: 10x 3' v3

text: Homo sapiens

text: female

characteristic: sex

text: reduction mammoplasty

characteristic: surgery type

characteristic: title


text: public

text: E-MTAB-13664_4:D32_Str_all_SLX-19864-20262_SIGAD10

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Stroma enriched - unsorted

characteristic: fraction

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

characteristic: organism part

characteristic: parity

text: 10x 3' v3

characteristic: protocol

text: Homo sapiens

text: female

text: prophylactic mastectomy


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13664_4:D53_Str_all_SLX-20870_SIGAD9

characteristic: Submitter Id

characteristic: age

characteristic: batch

text: ArrayExpress

text: not collected

text: human

text: adult

text: normal

text: Stroma enriched - unsorted

text: not collected

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: breast

text: 10x 3' v3

characteristic: sample id

text: Homo sapiens

text: female

text: contralateral


text: public

text: E-MTAB-13664_4:D8_Epi_LASP_SLX-19864-20262_SIGAH4

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Epithelial enriched - LASP-sorted

characteristic: fraction

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

text: 10x 3' v3

text: Homo sapiens

text: female

characteristic: sex

text: reduction mammoplasty

characteristic: surgery type

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13664_4:D28_Str_all_SLX-20261_SIGAA6

characteristic: Submitter Id

characteristic: age

characteristic: batch

text: ArrayExpress

text: not collected

text: human

text: adult

text: normal

text: Stroma enriched - unsorted

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

characteristic: organism part

characteristic: parity

text: 10x 3' v3

characteristic: protocol

text: Homo sapiens

text: female

text: prophylactic mastectomy


text: public

text: E-MTAB-13664_4:D21_Str_all_SLX-20261-20448_SIGAG10

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Stroma enriched - unsorted

characteristic: fraction

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: breast

text: 10x 3' v3

characteristic: sample id

text: Homo sapiens

text: female

text: reduction mammoplasty


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13664_4:D47_Epi_LASP_SLX-20870_SIGAF9

characteristic: Submitter Id

characteristic: age

characteristic: batch

text: ArrayExpress

text: not collected

text: human

text: adult

text: normal

text: Epithelial enriched - LASP-sorted

text: unknown

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

text: 10x 3' v3

text: Homo sapiens

text: female

characteristic: sex

text: prophylactic mastectomy

characteristic: surgery type

characteristic: title


text: public

text: E-MTAB-13664_4:D9_Epi_LASP_SLX-20261-20448_SIGAD10

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Epithelial enriched - LASP-sorted

characteristic: fraction

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

characteristic: organism part

characteristic: parity

text: 10x 3' v3

characteristic: protocol

text: Homo sapiens

text: female

text: reduction mammoplasty


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13664_4:D4_Epi_all_SLX-19864_SIGAD7

characteristic: Submitter Id

characteristic: age

characteristic: batch

text: ArrayExpress

text: not collected

text: human

text: adult

text: normal

text: Epithelial enriched - unsorted

text: not collected

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: breast

text: 10x 3' v3

characteristic: sample id

text: Homo sapiens

text: female

text: reduction mammoplasty


text: public

text: E-MTAB-13664_4:failed_1_Str_all_SLX-20261_SIGAF10

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Stroma enriched - unsorted

characteristic: fraction

text: unknown

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

text: 10x 3' v3

text: Homo sapiens

text: female

characteristic: sex

text: prophylactic mastectomy

characteristic: surgery type

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13664_4:D47_Str_all_SLX-20870_SIGAG9

characteristic: Submitter Id

characteristic: age

characteristic: batch

text: ArrayExpress

text: not collected

text: human

text: adult

text: normal

text: Stroma enriched - unsorted

text: unknown

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

characteristic: organism part

characteristic: parity

text: 10x 3' v3

characteristic: protocol

text: Homo sapiens

text: female

text: prophylactic mastectomy


text: public

text: E-MTAB-13664_4:D10_Epi_LASP_SLX-20261_SIGAF2

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Epithelial enriched - LASP-sorted

characteristic: fraction

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: breast

text: 10x 3' v3

characteristic: sample id

text: Homo sapiens

text: female

text: reduction mammoplasty


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13664_4:D24_Str_all_SLX-20870_SIGAA9

characteristic: Submitter Id

characteristic: age

characteristic: batch

text: ArrayExpress

text: not collected

text: human

text: Protocols: All frozen primary human breast tissue was obtained from the Breast Cancer Now Tissue bank (REC 15/EE/0192). Prior to freezing, fresh tissues were minced and digested O/N in 1 mg/mL Collagenase 1A and 1 mg/mL Hyaluronidase in an orbital shaker, washed 2-3 times and sedimented to allow for the separation between epithelial-enriched and stromal fractions, which were then separately cryostored. Frozen vials of epithelial-enriched or stromal-enriched fractions were defrosted, washed in PBS and centrifuged at 400 g for 5 minutes and resuspended in 2 mL of freshly prepared PBS + 0.025% Trypsin and 0.4 mg/mL Deoxyribonuclease 1 (DNase). Samples were then incubated at 37°C with pipetting up and down for 2-3 minutes until smoothly digested or up to a maximum of 10 minutes. Next, samples were washed and centrifuged for 20 minutes at 400 g with slow break. The pellet was resuspended in media + DNase until homogeneous, then diluted and filtered through a 40 μm cell strainer. After centrifugation for 5 minutes at 400 g, the pellet was resuspended by pipetting up and down in 200 μL of Cell Preparation Medium + 10 μL of 10 mg/mL DNase until homogeneous, then washed in 3-6 mL of media. 30,000 cells were resuspended into 48 μL of media and 400 Human mammary epithelial cells (HuMECs, Thermo Fisher Scientific A10565) were added as spike-in, and samples were submitted for scRNA-seq (either stromal-enriched or epithelial-enriched samples). For the epithelial-enriched fraction only, the rest of the processed sample was stained with primary antibodies: CD45-APC, CD31-APC, EPCAM-AF488, CD49f-PE/Cy7. DAPI was used to detect dead cells. Cells were filtered through a cell strainer before sorting. Sorting of cells was done using a FACS Aria Fusion sorter. Single-stained control cells were used to perform compensation manually and unstained cells were used to set gates. After doublets, dead cells and contaminating haematopoietic and endothelial cells (referred to as lineage) were gated, up to 30,000 LASPs were sorted for scRNA-seq (with the addition of 400 HuMECs as spike-in). Approximately 30,000 mammary cells per sample were loaded into separate channels of a single Chromium controller (10x Genomics) chip. Consequently, gel bead-in-emulsions (GEMS) were generated using 10X Genomics Single Cell 3’ Reagent Kit v3. Sequencing libraries were prepared using Single Cell 3’ Reagent Kits v3 (10X Genomics) and then converted using the NovaSeq 6000 S2 Reagent Kit (Illumina).

text: adult

text: normal

text: Stroma enriched - unsorted

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

text: 10x 3' v3

text: Homo sapiens

text: female

characteristic: sex

text: prophylactic mastectomy

characteristic: surgery type

characteristic: title


text: Cambridge Stem Cell Institute Department of Pharmacology

text: public

text: E-MTAB-13664_4:D16_Epi_LASP_SLX-20261_SIGAD3

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Epithelial enriched - LASP-sorted

characteristic: fraction

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

characteristic: organism part

characteristic: parity

text: 10x 3' v3

characteristic: protocol

text: Homo sapiens

text: female

text: reduction mammoplasty


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13664_4:D42_Str_all_SLX-20005_SIGAA12

characteristic: Submitter Id

characteristic: age

characteristic: batch

text: ArrayExpress

text: not collected

text: human

text: adult

text: normal

text: Stroma enriched - unsorted

text: not collected

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: breast

text: 10x 3' v3

characteristic: sample id

text: Homo sapiens

text: female

text: prophylactic mastectomy


text: public

text: E-MTAB-13664_4:D3_Str_all_SLX-20261_SIGAF6

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Stroma enriched - unsorted

characteristic: fraction

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

text: unknown

text: 10x 3' v3

text: Homo sapiens

text: female

characteristic: sex

text: reduction mammoplasty

characteristic: surgery type

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13664_4:D20_Epi_all_SLX-19864_SIGAC2

characteristic: Submitter Id

characteristic: age

characteristic: batch

text: ArrayExpress

text: not collected

text: human

text: adult

text: normal

text: Epithelial enriched - unsorted

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

characteristic: organism part

characteristic: parity

text: 10x 3' v3

characteristic: protocol

text: Homo sapiens

text: female

text: reduction mammoplasty


text: public

text: E-MTAB-13664_4:failed_2_Epi_all_SLX-20005_SIGAC9

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Epithelial enriched - unsorted

characteristic: fraction

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: breast

text: 10x 3' v3

characteristic: sample id

text: Homo sapiens

text: female

text: reduction mammoplasty


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13664_4:D11_Epi_all_SLX-20005_SIGAE9

characteristic: Submitter Id

characteristic: age

characteristic: batch

text: ArrayExpress

text: not collected

text: human

text: adult

text: normal

text: Epithelial enriched - unsorted

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

text: 10x 3' v3

text: Homo sapiens

text: female

characteristic: sex

text: reduction mammoplasty

characteristic: surgery type

characteristic: title


text: public

text: E-MTAB-13664_4:D16_Epi_all_SLX-20261_SIGAC3

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Epithelial enriched - unsorted

characteristic: fraction

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

characteristic: organism part

characteristic: parity

text: 10x 3' v3

characteristic: protocol

text: Homo sapiens

text: female

text: reduction mammoplasty


characteristic: ENA-CHECKLIST

text: University of Oxford

characteristic: GAL

text: NOT PROVIDED

text: Tree of Life Programme

characteristic: INSDC center name

characteristic: SRA accession

characteristic: Submitter Id

text: NATURAL HISTORY MUSEUM

characteristic: barcoding center

text: Liam Crowley | James McCulloch

text: University of Oxford

characteristic: collecting institution

unit: m

characteristic: elevation

text: United Kingdom

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Oxfordshire | Withymead Nature Reserve

characteristic: geographic location (region and locality)

text: Reedbed

characteristic: habitat

text: Tuberolachnus salignus

characteristic: host scientific name

characteristic: host taxid

text: Liam Crowley

text: University of Oxford

text: adult

characteristic: lifestage

text: Candidatus Hamiltonella endosymbiont of Tuberolachnus salignus

characteristic: organism

text: WHOLE ORGANISM

text: DTOL

characteristic: project name

text: NOT APPLICABLE

characteristic: proxy voucher

text: Sweep net

characteristic: sample collection method

characteristic: sample symbiont of

text: FEMALE

text: Y

characteristic: symbiont

text: eee46e84-88ba-4997-9627-ba9a3c08c7b3-dtol-cobiont

characteristic: tolid

type: derived from


characteristic: ENA-CHECKLIST

text: University of Oxford

characteristic: GAL

text: NOT PROVIDED

text: Tree of Life Programme

characteristic: INSDC center name

characteristic: SRA accession

characteristic: Submitter Id

text: NATURAL HISTORY MUSEUM

characteristic: barcoding center

text: Liam Crowley | James McCulloch

text: University of Oxford

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Oxfordshire | Withymead Nature Reserve

text: Reedbed

text: Tuberolachnus salignus

text: Liam Crowley

text: University of Oxford

text: adult

text: Candidatus Regiella endosymbiont of Tuberolachnus salignus

text: WHOLE ORGANISM

characteristic: organism part

text: DTOL

text: NOT APPLICABLE

text: Sweep net

text: FEMALE

characteristic: sex

text: Y

text: 50df7a5c-45bd-4916-af13-c11c5892e7e1-dtol-cobiont

characteristic: title


text: University of Oxford

text: NOT PROVIDED

text: Tree of Life Programme

text: NATURAL HISTORY MUSEUM

text: Liam Crowley | James McCulloch

text: University of Oxford

characteristic: collecting institution

characteristic: elevation

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Oxfordshire | Withymead Nature Reserve

characteristic: geographic location (region and locality)

text: Reedbed

characteristic: habitat

text: Tuberolachnus salignus

characteristic: host scientific name

characteristic: host taxid

text: Liam Crowley

text: University of Oxford

text: adult

characteristic: lifestage

text: Serratia symbiotica (Tuberolachnus salignus)

characteristic: organism

text: WHOLE ORGANISM

text: DTOL

characteristic: project name

text: NOT APPLICABLE

text: Sweep net

text: FEMALE

text: Y

text: 9fdd06ab-7e51-4012-94e9-ef92e41eb10f-dtol-cobiont


characteristic: ENA-CHECKLIST

text: Natural History Museum

characteristic: GAL

text: NOT PROVIDED

text: Tree of Life Programme

characteristic: INSDC center name

characteristic: SRA accession

characteristic: Submitter Id

text: MAXWELL BARCLAY

text: NATURAL HISTORY MUSEUM

text: United Kingdom

text: not collected

text: not collected

text: England| Leatherhead|Bookham Common

text: NOT COLLECTED

text: Agriotes lineatus

text: MAXWELL BARCLAY

text: NATURAL HISTORY MUSEUM

text: adult

text: Spiroplasma endosymbiont of Agriotes lineatus

text: WHOLE ORGANISM

text: DTOL

characteristic: sample symbiont of

text: NOT COLLECTED

text: NOT PROVIDED

text: Y

characteristic: symbiont

text: c80ccc06-2dea-4acd-bd14-5b1b8c50ad68-dtol-cobiont

characteristic: tolid


text: University of Oxford

text: NOT PROVIDED

text: Tree of Life Programme

text: Steven Falk

text: University of Oxford

characteristic: collecting institution

characteristic: collection date

characteristic: elevation

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Berkshire | Wytham woods

text: Woodland

text: Pipizella viduata

text: Steven Falk

text: University of Oxford

text: adult

text: Alphaproteobacteria bacterium endosymbiont of Pipizella viduata

text: WHOLE ORGANISM

characteristic: organism part

text: DTOL

text: NOT COLLECTED

characteristic: sex

text: NOT APPLICABLE

text: Y

text: e6689bee-e4fc-4eb7-97e8-416618babde4-dtol-cobiont

characteristic: title


text: Natural History Museum

text: NOT PROVIDED

text: Tree of Life Programme

text: MAXWELL BARCLAY

text: NATURAL HISTORY MUSEUM

text: United Kingdom

text: not collected

text: not collected

characteristic: geographic location (longitude)

text: England| Leatherhead|Bookham Common

characteristic: geographic location (region and locality)

text: NOT COLLECTED

characteristic: habitat

text: Agriotes lineatus

characteristic: host scientific name

characteristic: host taxid

text: MAXWELL BARCLAY

text: NATURAL HISTORY MUSEUM

text: adult

characteristic: lifestage

text: Candidatus Mesenet endosymbiont of Agriotes lineatus

characteristic: organism

text: WHOLE ORGANISM

text: DTOL

characteristic: project name

text: NOT COLLECTED

text: NOT PROVIDED

text: Y

text: 423b1271-2d71-4c33-aa66-f74c97ded29e-dtol-cobiont


characteristic: ENA-CHECKLIST

text: Natural History Museum

characteristic: GAL

text: NOT PROVIDED

text: Tree of Life Programme

characteristic: INSDC center name

characteristic: SRA accession

characteristic: Submitter Id

text: NATURAL HISTORY MUSEUM

characteristic: barcoding center

text: CLARE BOYES

text: DIPTERIST FORUM

text: United Kingdom

text: England|Buxton Heath

text: NOT COLLECTED

text: Hedychridium roseum

text: CLARE BOYES

text: DIPTERIST FORUM

text: adult

text: Candidatus Lariskella endosymbiont of Hedychridium roseum

text: WHOLE ORGANISM

text: DTOL

text: NOT APPLICABLE

characteristic: proxy voucher

text: Hand Picked

characteristic: sample collection method

characteristic: sample symbiont of

text: FEMALE

text: Y

characteristic: symbiont

text: 28e0f223-7833-4d3d-9dd7-acf0206c828b-dtol-cobiont

characteristic: tolid


text: Sanger Institute

text: NOT PROVIDED

text: Tree of Life Programme

text: NATURAL HISTORY MUSEUM

text: STEPHEN MORAN | ANDY GRIFFITHS

text: HIGHLAND BIOLOGICAL RECORDING GROUP | SANGER

characteristic: collecting institution

characteristic: collection date

text: alder spittlebug

characteristic: common name

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: SCOTLAND | HIGHLANDS | BEINN EIGHE NNR | COLLIE NA GLAS-LEITIR

characteristic: geographic location (region and locality)

text: caledonian pinewood

characteristic: habitat

text: Aphrophora alni

text: STEPHEN MORAN

text: HIGHLAND BIOLOGICAL RECORDING GROUP

text: adult

text: Candidatus Zinderia endosymbiont of Aphrophora alni

text: WHOLE ORGANISM

characteristic: organism part

text: DTOL

text: NOT COLLECTED

characteristic: sex

text: NOT PROVIDED

text: Y

text: cdd75adb-845e-4293-b7a8-8a044faef570-dtol-cobiont

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: GIMR

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Richard Pillans

text: CSIRO

text: speartooth shark

text: Blood sample from glyphis glyphis

characteristic: description

text: Australia

text: Wenlock river, Queensland

text: river/estuarine

text: juvenile

characteristic: lifestage

text: Glyphis glyphis

characteristic: organism

text: blood

text: ORG.one

characteristic: project name

text: Glyphis glyphis

text: female


text: soil metagenome


text: LONDON SCHOOL OF HYGIENE AND TROPICAL MEDICINE

text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:352-274480

characteristic: collection date

text: human

characteristic: common name

text: South Africa

characteristic: geographic location (country and/or sea)

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:353-274481

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:353-274482

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:353-274483

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:353-274484

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:353-274485

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:353-274486

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:353-274487

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:353-274488

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:353-274489

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:353-274490

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:353-274491

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:353-274492

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:353-274493

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:353-274494

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:353-274495

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: LONDON SCHOOL OF HYGIENE AND TROPICAL MEDICINE

text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:353-274496

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:353-274497

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:353-274498

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:353-274499

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:353-274500

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:353-274501

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:353-274502

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:353-274503

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:353-274504

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:353-274505

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:353-274506

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:354-274507

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:354-274508

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:354-274509

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:354-274510

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:354-274511

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: LONDON SCHOOL OF HYGIENE AND TROPICAL MEDICINE

text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:354-274512

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:354-274513

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:354-274514

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:354-274515

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:354-274516

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:354-274517

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:354-274518

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:354-274519

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:354-274520

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:354-274521

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:354-274522

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:354-274523

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:354-274524

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:354-274525

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:354-274526

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:354-274527

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: LONDON SCHOOL OF HYGIENE AND TROPICAL MEDICINE

text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:354-274528

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:354-274529

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:354-274530

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:354-274531

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:354-274532

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:354-274533

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:355-274534

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:355-274535

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:355-274536

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:355-274537

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:355-274538

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:355-274539

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:355-274540

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:355-274541

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:355-274542

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:355-274543

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: LONDON SCHOOL OF HYGIENE AND TROPICAL MEDICINE

text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:355-274544

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:355-274545

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:355-274546

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:355-274547

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:355-274548

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:355-274549

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: public

text: ena-SAMPLE-TAB-05-04-2024-11:39:20:355-274550

text: human

text: South Africa

text: Homo sapiens

text: Homo sapiens


text: FZB

text: public

text: WGS of MAC isolates from Europe

text: Germany

text: Mycobacterium avium

text: Mycobacterium avium


text: FZB

text: public

text: WGS of MAC isolates from Europe

text: Germany

text: Mycobacterium intracellulare subsp. chimaera


text: FZB

text: public

text: WGS of MAC isolates from Europe

text: Germany

text: Mycobacterium marseillense

text: Mycobacterium marseillense


text: FZB

text: public

text: WGS of MAC isolates from Europe

text: Germany

text: Mycobacterium intracellulare

text: Mycobacterium intracellulare


text: FZB

text: public

text: WGS of MAC isolates from Europe

text: Switzerland

text: Mycobacterium avium

text: Mycobacterium avium


text: FZB

text: public

text: WGS of MAC isolates from Europe

text: Switzerland


text: FZB

text: public

text: WGS of MAC isolates from Europe

text: Switzerland

text: Mycobacterium intracellulare

text: Mycobacterium intracellulare


text: FZB

text: public

text: WGS of MAC isolates from Europe

text: Switzerland

text: Mycobacterium marseillense

text: Mycobacterium marseillense


text: FZB

text: public

text: WGS of MAC isolates from Europe

text: Germany

text: Mycobacterium colombiense

text: Mycobacterium colombiense


text: FZB

text: public

text: WGS of MAC isolates from Europe

text: France

text: Mycobacterium intracellulare

text: Mycobacterium intracellulare


text: FZB

text: public

text: WGS of MAC isolates from Europe

text: France

text: Mycobacterium avium

text: Mycobacterium avium


text: FZB

text: public

text: WGS of MAC isolates from Europe

text: France


text: St James's Hopsital

text: Ireland

text: not collected

characteristic: host health state

text: homo sapiens

characteristic: host scientific name

characteristic: isolate

text: clinical sample

text: Mycobacteroides abscessus

text: Mycobacteroides abscessus


text: yes

text: NATIONAL INSTITUTE OF HEALTH DR. RICARDO JORGE

text: public

characteristic: Sample

text: clinical

characteristic: Source

text: Portugal

text: Homo sapiens

text: Listeria monocytogenes

text: Listeria monocytogenes

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Clinical Bioinformatics Area

characteristic: INSDC center name

text: public

text: ena-upload-cli

text: Servicio de Microbiologia, Hospital Universitario Virgen del Rocio, Sevilla, Spain

characteristic: collecting institution

text: not provided

characteristic: collector name

text: Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region. Sequencing supported by RELECOV consortium, funded by the European Centre for Diseases Prevention and Control (ECDC) under Grant Agreement ECDC/HERA/2021/024 ECDC.12241

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

text: Homo Sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolation source host-associated

text: active surveillance in response to outbreak

characteristic: sample capture status

characteristic: sample storage conditions

text: AND39366, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


text: Clinical Bioinformatics Area

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collection date

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism

text: AND39368, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Clinical Bioinformatics Area

characteristic: INSDC center name

text: public

text: ena-upload-cli

characteristic: collecting institution

text: not provided

characteristic: collector name

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND39369, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


text: Clinical Bioinformatics Area

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

text: not provided

text: Spain

text: Human

characteristic: host common name

text: not provided

text: Homo Sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolation source host-associated

characteristic: sample capture status

characteristic: sample storage conditions

text: AND39370, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


text: Clinical Bioinformatics Area

text: public

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collection date

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism

text: AND39371, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Clinical Bioinformatics Area

characteristic: INSDC center name

text: public

text: ena-upload-cli

characteristic: collecting institution

text: not provided

characteristic: collector name

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND39373, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


text: Clinical Bioinformatics Area

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

text: not provided

text: Spain

text: Human

characteristic: host common name

text: not provided

text: Homo Sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolation source host-associated

characteristic: sample capture status

characteristic: sample storage conditions

text: AND39374, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


text: Clinical Bioinformatics Area

text: public

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collection date

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism

text: AND39375, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Clinical Bioinformatics Area

characteristic: INSDC center name

text: public

text: ena-upload-cli

characteristic: collecting institution

text: not provided

characteristic: collector name

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND39378, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


text: Clinical Bioinformatics Area

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

text: not provided

text: Spain

text: Human

characteristic: host common name

text: not provided

text: Homo Sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolation source host-associated

characteristic: sample capture status

characteristic: sample storage conditions

text: AND39379, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


text: Clinical Bioinformatics Area

text: public

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collection date

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism

text: AND39381, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Clinical Bioinformatics Area

characteristic: INSDC center name

text: public

text: ena-upload-cli

characteristic: collecting institution

text: not provided

characteristic: collector name

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND39382, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


text: Clinical Bioinformatics Area

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

text: not provided

text: Spain

text: Human

characteristic: host common name

text: not provided

text: Homo Sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolation source host-associated

characteristic: sample capture status

characteristic: sample storage conditions

text: AND39383, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


text: Clinical Bioinformatics Area

text: public

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collection date

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism

text: AND39385, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Clinical Bioinformatics Area

characteristic: INSDC center name

text: public

text: ena-upload-cli

text: Servicio de Microbiologia, Hospital Universitario Virgen del Rocio, Sevilla, Spain

characteristic: collecting institution

text: not provided

characteristic: collector name

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND39388, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


text: Clinical Bioinformatics Area

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

text: not provided

text: Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region. Sequencing supported by RELECOV consortium, funded by the European Centre for Diseases Prevention and Control (ECDC) under Grant Agreement ECDC/HERA/2021/024 ECDC.12241

text: Spain

text: Human

characteristic: host common name

text: not provided

text: Homo Sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolation source host-associated

text: active surveillance in response to outbreak

characteristic: sample capture status

characteristic: sample storage conditions

text: AND39390, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


text: Clinical Bioinformatics Area

text: public

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collection date

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism

text: AND39391, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Clinical Bioinformatics Area

characteristic: INSDC center name

text: public

text: ena-upload-cli

characteristic: collecting institution

text: not provided

characteristic: collector name

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND39392, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


text: Clinical Bioinformatics Area

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

text: not provided

text: Spain

text: Human

characteristic: host common name

text: not provided

text: Homo Sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolation source host-associated

characteristic: sample capture status

characteristic: sample storage conditions

text: AND39393, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


text: Clinical Bioinformatics Area

text: public

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collection date

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism

text: AND39398, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Clinical Bioinformatics Area

characteristic: INSDC center name

text: public

text: ena-upload-cli

characteristic: collecting institution

text: not provided

characteristic: collector name

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND39399, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


text: HUS

text: public

characteristic: INSDC status

characteristic: SRA accession

text: wound exudate

text: Norway

text: diseased

text: Ecuus ferus caballus

text: skin

text: Streptococcus dysgalactiae

text: Streptococcus dysgalactiae


text: Clinical Bioinformatics Area

text: public

text: ena-upload-cli

characteristic: Submitter Id

text: Servicio de Microbiologia, Hospital Puerta del Mar, Cadiz, Spain

characteristic: collection date

text: not provided

text: Spain

text: Human

characteristic: host common name

text: not provided

text: Homo Sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolation source host-associated

characteristic: sample capture status

characteristic: sample storage conditions

text: AND39499, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Clinical Bioinformatics Area

text: public

text: ena-upload-cli

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism

text: AND39500, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


text: Clinical Bioinformatics Area

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

characteristic: collecting institution

text: not provided

characteristic: collector name

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND39501, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


text: Clinical Bioinformatics Area

text: public

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collection date

text: not provided

text: Spain

text: Human

characteristic: host common name

text: not provided

text: Homo Sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolation source host-associated

characteristic: sample capture status

characteristic: sample storage conditions

text: AND39502, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Clinical Bioinformatics Area

text: public

text: ena-upload-cli

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism

text: AND39503, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


text: Clinical Bioinformatics Area

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

characteristic: collecting institution

text: not provided

characteristic: collector name

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND39504, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


text: Clinical Bioinformatics Area

text: public

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collection date

text: not provided

text: Spain

text: Human

characteristic: host common name

text: not provided

text: Homo Sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolation source host-associated

characteristic: sample capture status

characteristic: sample storage conditions

text: AND39505, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Clinical Bioinformatics Area

text: public

text: ena-upload-cli

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism

text: AND39506, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


text: Clinical Bioinformatics Area

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

characteristic: collecting institution

text: not provided

characteristic: collector name

text: Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region. Sequencing supported by RELECOV consortium, funded by the European Centre for Diseases Prevention and Control (ECDC) under Grant Agreement ECDC/HERA/2021/024 ECDC.12241

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND39507, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


text: Clinical Bioinformatics Area

text: public

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collection date

text: not provided

text: Spain

text: Human

characteristic: host common name

text: not provided

text: Homo Sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolation source host-associated

text: active surveillance in response to outbreak

characteristic: sample capture status

characteristic: sample storage conditions

text: AND39508, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Clinical Bioinformatics Area

text: public

text: ena-upload-cli

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism

text: AND39509, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


text: Clinical Bioinformatics Area

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

characteristic: collecting institution

text: not provided

characteristic: collector name

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND39510, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


text: Clinical Bioinformatics Area

text: public

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collection date

text: not provided

text: Spain

text: Human

characteristic: host common name

text: not provided

text: Homo Sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolation source host-associated

characteristic: sample capture status

characteristic: sample storage conditions

text: AND39511, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Clinical Bioinformatics Area

text: public

text: ena-upload-cli

text: Servicio de Microbiologia, Hospital Universitario Virgen del Rocio, Sevilla, Spain

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism

text: AND39512, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


text: Clinical Bioinformatics Area

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

characteristic: collecting institution

text: not provided

characteristic: collector name

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND39513, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


text: Clinical Bioinformatics Area

text: public

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collection date

text: not provided

text: Spain

text: Human

characteristic: host common name

text: not provided

text: Homo Sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolation source host-associated

characteristic: sample capture status

characteristic: sample storage conditions

text: AND39514, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Clinical Bioinformatics Area

text: public

text: ena-upload-cli

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism

text: AND39515, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


text: Clinical Bioinformatics Area

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

characteristic: collecting institution

text: not provided

characteristic: collector name

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND39516, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


text: Clinical Bioinformatics Area

text: public

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collection date

text: not provided

text: Spain

text: Human

characteristic: host common name

text: not provided

text: Homo Sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolation source host-associated

characteristic: sample capture status

characteristic: sample storage conditions

text: AND39517, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region