characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: environmental medium

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay59): AAGAACTCATGA; REV (BC54): TATCCTGGTTTC

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

text: not collected

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title

text: not collected


status: PUBLIC

characteristic: ENA first public

tag: attribute

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

unit: m

characteristic: depth

text: PHB incubation - pelagic habitat - 10 months

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

text: Sea Water [ENVO:00002149]|Plastisphere [ENVO:06105106]

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

unit: DD

text: Tyrrhenian Sea: Italy, Island of Pianosa, National Park Tuscan Archipelago

text: Coastal zone [ENVO:00000486]

text: FWD (Golay61): TGATATCGTCTT; REV (BC56): CTGGTGCTGAAT

text: Powersoil DNA Isolation kit

text: plastisphere metagenome

text: not collected

text: Initial denaturation: 98degC_30s; 30 cycles of denaturation: 98degC_10s, annealing: 50degC_15s, elongation: 72degC_30s; final elongation: 72degC_5min

text: FWD (515F-Y): GTGYCAGCMGCCGCGGTAA; REV (926R): CCGYCAATTYMTTTRAGTTT

characteristic: pcr primers

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

unit: psu

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

unit: ºC

characteristic: temperature

characteristic: total depth of water column

text: not collected

characteristic: total inorganic nitrogen (mg/L)

characteristic: total nitrogen (mg/L)

characteristic: total phosphorus (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: environmental medium

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay63): CGGTGACCTACT; REV (BC58): GTTGAAGCACCT

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

text: not collected

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title

text: not collected


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

text: Coastal zone [ENVO:00000486]

text: FWD (Golay65): AATGCGCGTATA; REV (BC60): GTGCGGTTCACT

text: Powersoil DNA Isolation kit

text: plastisphere metagenome

text: not collected

characteristic: pcr primers

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

characteristic: temperature

characteristic: total depth of water column

text: not collected

characteristic: total inorganic nitrogen (mg/L)

characteristic: total nitrogen (mg/L)

characteristic: total phosphorus (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

text: LDPE incubation - pelagic habitat - 10 months

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: environmental medium

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay67): CTTGATTCTTGA; REV (BC62): CTTGTGCGACAA

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

text: not collected

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title

text: not collected


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

text: Coastal zone [ENVO:00000486]

text: FWD (Golay69): GAAATCTTGAAG; REV (BC64): CACAGTTGAAGT

text: Powersoil DNA Isolation kit

text: plastisphere metagenome

text: not collected

characteristic: pcr primers

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

characteristic: temperature

characteristic: total depth of water column

text: not collected

characteristic: total inorganic nitrogen (mg/L)

characteristic: total nitrogen (mg/L)

characteristic: total phosphorus (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: environmental medium

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay71): GAGATACAGTTC; REV (BC66): GGCTCGTCGGAG

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

text: not collected

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title

text: not collected


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

text: not collected

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

text: PBSeT incubation - pelagic habitat - 22 months

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

text: Coastal zone [ENVO:00000486]

text: FWD (Golay73): GTGGAGTCTCAT; REV (BC68): ACATGGGCGGAA

text: Powersoil DNA Isolation kit

text: plastisphere metagenome

characteristic: pcr primers

text: not collected

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

characteristic: temperature

characteristic: total depth of water column

characteristic: total inorganic nitrogen (mg/L)

characteristic: total nitrogen (mg/L)

characteristic: total phosphorus (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

text: not collected

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: environmental medium

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay75): ACCTTACACCTT; REV (BC70): TGCGCGCCTTCC

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: not collected

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

text: not collected

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

text: Coastal zone [ENVO:00000486]

text: FWD (Golay77): TAATCTCGCCGG; REV (BC72): GTTGGTTGGCAT

text: Powersoil DNA Isolation kit

text: plastisphere metagenome

characteristic: pcr primers

text: not collected

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

characteristic: temperature

characteristic: total depth of water column

characteristic: total inorganic nitrogen (mg/L)

characteristic: total nitrogen (mg/L)

characteristic: total phosphorus (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

text: not collected

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

text: PHB incubation - pelagic habitat - 22 months

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: environmental medium

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay79): ATCTAGTGGCAA; REV (BC74): GAACGATCATGT

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: not collected

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

text: not collected

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

text: LDPE incubation - pelagic habitat - 22 months

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

text: Sea Water [ENVO:00002149]|Plastisphere [ENVO:06105106]

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

text: Coastal zone [ENVO:00000486]

text: FWD (Golay81): ACGCTTAACGAC; REV (BC76): TCAGTCAGATGA

text: Powersoil DNA Isolation kit

text: plastisphere metagenome

characteristic: pcr primers

text: not collected

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

characteristic: temperature

characteristic: total depth of water column

characteristic: total inorganic nitrogen (mg/L)

characteristic: total nitrogen (mg/L)

characteristic: total phosphorus (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

text: not collected

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: environmental medium

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Tyrrhenian Sea: Italy, Island of Pianosa, National Park Tuscan Archipelago

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay83): TACGGATTATGG; REV (BC78): CTGGTCTTACGG

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

characteristic: particulate organic carbon (mg/L)

text: Initial denaturation: 98degC_30s; 30 cycles of denaturation: 98degC_10s, annealing: 50degC_15s, elongation: 72degC_30s; final elongation: 72degC_5min

characteristic: pcr conditions

text: not collected

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

text: PBSeT incubation - benthic habitat - 2.5 months

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: elevation

text: Marine sediment [ENVO:03000033]|Plastisphere [ENVO:06105106]

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

text: Coastal zone [ENVO:00000486]

text: FWD (Golay85): ATACATGCAAGA; REV (BC80): ACAAAGGTATCA

text: Powersoil DNA Isolation kit

text: not collected

text: plastisphere metagenome

text: not collected

text: FWD (515F-Y): GTGYCAGCMGCCGCGGTAA; REV (926R): CCGYCAATTYMTTTRAGTTT

characteristic: pcr primers

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

characteristic: temperature

characteristic: total carbon (mg/L)

characteristic: total nitrogen (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay87): CTTAGTGCAGAA; REV (BC82): GTAAACGACTTG

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

text: not collected

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

text: not collected

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: elevation

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

text: Coastal zone [ENVO:00000486]

text: FWD (Golay89): AATCTTGCGCCG; REV (BC84): TAGCGCGAACTT

text: Powersoil DNA Isolation kit

text: not collected

text: plastisphere metagenome

text: not collected

characteristic: pcr primers

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

characteristic: temperature

characteristic: total carbon (mg/L)

characteristic: total nitrogen (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

text: PHB incubation - benthic habitat - 2.5 months

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay91): AGGATCAGGGAA; REV (BC86): GCGCTTAGAATA

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

text: not collected

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

text: not collected

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: elevation

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

text: Coastal zone [ENVO:00000486]

text: FWD (Golay93): AATAACTAGGGT; REV (BC88): GCAAAGGCCCGC

text: Powersoil DNA Isolation kit

text: not collected

text: plastisphere metagenome

text: not collected

characteristic: pcr primers

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

characteristic: temperature

characteristic: total carbon (mg/L)

characteristic: total nitrogen (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay95): TATTGCAGCAGC; REV (BC90): GACATCTGACAC

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

text: not collected

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

text: not collected

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

text: LDPE incubation - benthic habitat - 2.5 months

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: elevation

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

text: Coastal zone [ENVO:00000486]

text: FWD (Golay97): TGATGTGCTAAG; REV (BC92): CTGCGGATATAC

text: Powersoil DNA Isolation kit

text: not collected

text: plastisphere metagenome

text: not collected

characteristic: pcr primers

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

characteristic: temperature

characteristic: total carbon (mg/L)

characteristic: total nitrogen (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay99): GTAGTAGACCAT; REV (BC94): GCGCACACCTTC

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

text: not collected

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

text: not collected

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: elevation

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

text: Coastal zone [ENVO:00000486]

text: FWD (Golay101): AGTAAAGATCGT; REV (BC96): AACGGGCGACGT

text: Powersoil DNA Isolation kit

text: not collected

text: plastisphere metagenome

text: not collected

characteristic: pcr primers

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

characteristic: temperature

characteristic: total carbon (mg/L)

characteristic: total nitrogen (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

text: PBSeT incubation - benthic habitat - 5 months

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Tyrrhenian Sea: Italy, Island of Pianosa, National Park Tuscan Archipelago

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay103): CTCGCCCTCGCC; REV (BC98): CTCCACATTCCT

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

text: not collected

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

text: not collected

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: elevation

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

text: Coastal zone [ENVO:00000486]

text: FWD (Golay105): TCTCTTTCGACA; REV (BC100): CGAACGTCTATG

text: Powersoil DNA Isolation kit

text: not collected

text: plastisphere metagenome

text: not collected

text: Initial denaturation: 98degC_30s; 30 cycles of denaturation: 98degC_10s, annealing: 50degC_15s, elongation: 72degC_30s; final elongation: 72degC_5min

characteristic: pcr primers

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

characteristic: temperature

characteristic: total carbon (mg/L)

characteristic: total nitrogen (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

text: Marine sediment [ENVO:03000033]|Plastisphere [ENVO:06105106]

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay107): ACATACTGAGCA; REV (BC102): ATGCCGGTAATA

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

text: not collected

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

text: not collected

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

text: PHB incubation - benthic habitat - 5 months

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: elevation

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

text: Coastal zone [ENVO:00000486]

text: FWD (Golay109): GTTGATACGATG; REV (BC104): TGAACCCTATGG

text: Powersoil DNA Isolation kit

text: not collected

text: plastisphere metagenome

text: not collected

text: FWD (515F-Y): GTGYCAGCMGCCGCGGTAA; REV (926R): CCGYCAATTYMTTTRAGTTT

characteristic: pcr primers

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

characteristic: temperature

characteristic: total carbon (mg/L)

characteristic: total nitrogen (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay111): GTCAACGCTGTC; REV (BC106): TTGGACGTCCAC

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

text: not collected

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

text: not collected

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: elevation

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

text: Coastal zone [ENVO:00000486]

text: FWD (Golay113): TGAGACCCTACA; REV (BC108): ATGTAGGCTTAG

text: Powersoil DNA Isolation kit

text: not collected

text: plastisphere metagenome

text: not collected

characteristic: pcr primers

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

characteristic: temperature

characteristic: total carbon (mg/L)

characteristic: total nitrogen (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

text: LDPE incubation - benthic habitat - 5 months

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay115): ACTTGGTGTAAG; REV (BC110): AGAGGAGTCGAC

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

text: not collected

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

text: not collected

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: elevation

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

text: Coastal zone [ENVO:00000486]

text: FWD (Golay117): ATTACGTATCAT; REV (BC112): CTCCCACTAGAG

text: Powersoil DNA Isolation kit

text: not collected

text: plastisphere metagenome

text: not collected

characteristic: pcr primers

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

characteristic: temperature

characteristic: total carbon (mg/L)

characteristic: total nitrogen (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay119): CACGCAGTCTAC; REV (BC114): AATTTCCTAACA

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

text: not collected

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

text: not collected

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

text: PBSeT incubation - benthic habitat - 7.5 months

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: elevation

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

text: Coastal zone [ENVO:00000486]

text: FWD (Golay121): TGTGCACGCCAT; REV (BC116): GTGAGGGCAAGT

text: Powersoil DNA Isolation kit

text: plastisphere metagenome

characteristic: pcr primers

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

characteristic: temperature

characteristic: total carbon (mg/L)

characteristic: total nitrogen (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Tyrrhenian Sea: Italy, Island of Pianosa, National Park Tuscan Archipelago

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay123): CCGGACAAGAAG; REV (BC118): CACGAAAGCAGG

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: elevation

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

text: Coastal zone [ENVO:00000486]

text: FWD (Golay125): TTGCTGGACGCT; REV (BC120): TACTGAGCCTCG

text: Powersoil DNA Isolation kit

text: plastisphere metagenome

characteristic: pcr primers

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

characteristic: temperature

characteristic: total carbon (mg/L)

characteristic: total nitrogen (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

text: PHB incubation - benthic habitat - 7.5 months

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay127): TACTAACGCGGT; REV (BC122): TCTTCGCAGCAG

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

characteristic: particulate organic carbon (mg/L)

text: Initial denaturation: 98degC_30s; 30 cycles of denaturation: 98degC_10s, annealing: 50degC_15s, elongation: 72degC_30s; final elongation: 72degC_5min

characteristic: pcr conditions

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: elevation

text: Marine sediment [ENVO:03000033]|Plastisphere [ENVO:06105106]

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

text: Coastal zone [ENVO:00000486]

text: FWD (Golay129): GCGATCACACCT; REV (BC124): CAGTCCCTGCAC

text: Powersoil DNA Isolation kit

text: plastisphere metagenome

characteristic: pcr primers

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

characteristic: temperature

characteristic: total carbon (mg/L)

characteristic: total nitrogen (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay131): CAAACGCACTAA; REV (BC126): ATCAAGATACGC

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: FWD (515F-Y): GTGYCAGCMGCCGCGGTAA; REV (926R): CCGYCAATTYMTTTRAGTTT

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

text: LDPE incubation - benthic habitat - 7.5 months

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: elevation

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

text: Coastal zone [ENVO:00000486]

text: FWD (Golay133): GAAGAGGGTTGA; REV (BC128): AATAAGCAATAG

text: Powersoil DNA Isolation kit

text: plastisphere metagenome

characteristic: pcr primers

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

characteristic: temperature

characteristic: total carbon (mg/L)

characteristic: total nitrogen (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay137): TTACACAAAGGC; REV (BC132): GCATTACTGGAC

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

text: PBSeT incubation - benthic habitat - 10 months

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: elevation

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

text: Coastal zone [ENVO:00000486]

text: FWD (Golay139): ACGACGCATTTG; REV (BC134): GTCTCCTCCCTT

text: Powersoil DNA Isolation kit

text: plastisphere metagenome

text: not collected

characteristic: pcr primers

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

characteristic: temperature

characteristic: total carbon (mg/L)

characteristic: total nitrogen (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay141): TATCCAAGCGCA; REV (BC136): AATAGATGCTGA

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

text: not collected

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: elevation

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

text: Coastal zone [ENVO:00000486]

text: FWD (Golay143): AGAGCCAAGAGC; REV (BC138): ATAAACGGACAT

text: Powersoil DNA Isolation kit

text: plastisphere metagenome

text: not collected

characteristic: pcr primers

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

characteristic: temperature

characteristic: total carbon (mg/L)

characteristic: total nitrogen (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

text: PHB incubation - benthic habitat - 10 months

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Tyrrhenian Sea: Italy, Island of Pianosa, National Park Tuscan Archipelago

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay145): GGTGAGCAAGCA; REV (BC140): ATATTGGCAGCC

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

text: not collected

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: elevation

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

text: Coastal zone [ENVO:00000486]

text: FWD (Golay147): TAAATATACCCT; REV (BC142): CGTGGCTTTCCG

text: Powersoil DNA Isolation kit

text: plastisphere metagenome

text: not collected

characteristic: pcr primers

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

characteristic: temperature

characteristic: total carbon (mg/L)

characteristic: total nitrogen (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay149): TTGCGGACCCTA; REV (BC144): GGTGCAGACAGA

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

text: not collected

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

text: LDPE incubation - benthic habitat - 10 months

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: elevation

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

text: Coastal zone [ENVO:00000486]

text: FWD (Golay151): GTCGTCCAAATG; REV (BC146): CACGCTATTGGA

text: Powersoil DNA Isolation kit

text: plastisphere metagenome

text: not collected

text: Initial denaturation: 98degC_30s; 30 cycles of denaturation: 98degC_10s, annealing: 50degC_15s, elongation: 72degC_30s; final elongation: 72degC_5min

characteristic: pcr primers

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

characteristic: temperature

characteristic: total carbon (mg/L)

characteristic: total nitrogen (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

text: Marine sediment [ENVO:03000033]|Plastisphere [ENVO:06105106]

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay153): TGCACAGTCGCT; REV (BC148): TGATTTAATTGC

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

text: not collected

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: elevation

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

text: Coastal zone [ENVO:00000486]

text: FWD (Golay155): TTACTGTGGCCG; REV (BC150): CTGAGGCCCTTC

text: Powersoil DNA Isolation kit

text: plastisphere metagenome

text: not collected

text: FWD (515F-Y): GTGYCAGCMGCCGCGGTAA; REV (926R): CCGYCAATTYMTTTRAGTTT

characteristic: pcr primers

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

characteristic: temperature

characteristic: total carbon (mg/L)

characteristic: total nitrogen (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

text: PBSeT incubation - benthic habitat - 22 months

characteristic: description

characteristic: dissolved organic carbon (mg/L)

text: not collected

characteristic: dissolved oxygen (mg/L)

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay157): GGTTCATGAACA; REV (BC152): AGAAGGCCTTAT

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

text: not collected

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

text: not collected

characteristic: pH ()

text: not collected

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: not collected

text: Open-Bio bioplastics

text: not collected

characteristic: salinity (psu)

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

text: not collected

characteristic: temperature (ºC)

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

text: not collected

characteristic: dissolved oxygen (mg/L)

characteristic: elevation

characteristic: environmental medium

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

text: Coastal zone [ENVO:00000486]

text: FWD (Golay159): TAACAATAATTC; REV (BC154): CTTAGGCATGTG

text: Powersoil DNA Isolation kit

text: not collected

text: plastisphere metagenome

text: not collected

text: not collected

characteristic: pcr primers

text: not collected

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

text: not collected

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

text: not collected

characteristic: total carbon (mg/L)

characteristic: total nitrogen (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

text: PHB incubation - benthic habitat - 22 months

characteristic: description

characteristic: dissolved organic carbon (mg/L)

text: not collected

characteristic: dissolved oxygen (mg/L)

text: Field incubation, Time series

text: Mediterranean Sea

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay161): CTTATTAAACGT; REV (BC156): TCCGGACTCCTG

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

text: not collected

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

text: not collected

characteristic: pH ()

text: not collected

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: not collected

text: Open-Bio bioplastics

text: not collected

characteristic: salinity (psu)

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

text: not collected

characteristic: temperature (ºC)

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

text: not collected

characteristic: dissolved oxygen (mg/L)

characteristic: elevation

characteristic: environmental medium

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

text: Coastal zone [ENVO:00000486]

text: FWD (Golay163): GCTCGAAGATTC; REV (BC158): AGTATCATATAT

text: Powersoil DNA Isolation kit

text: not collected

text: plastisphere metagenome

text: not collected

text: not collected

characteristic: pcr primers

text: not collected

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

text: not collected

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

text: not collected

characteristic: total carbon (mg/L)

characteristic: total nitrogen (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

text: LDPE incubation - benthic habitat - 22 months

characteristic: description

characteristic: dissolved organic carbon (mg/L)

text: not collected

characteristic: dissolved oxygen (mg/L)

text: Field incubation, Time series

text: Mediterranean Sea

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Tyrrhenian Sea: Italy, Island of Pianosa, National Park Tuscan Archipelago

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay165): TATTTGATTGGT; REV (BC160): AACGCTTCTTAT

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

text: not collected

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

text: not collected

characteristic: pH ()

text: not collected

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: not collected

text: Open-Bio bioplastics

text: not collected

characteristic: salinity (psu)

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

text: not collected

characteristic: temperature (ºC)

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

text: not collected

characteristic: dissolved oxygen (mg/L)

characteristic: elevation

characteristic: environmental medium

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

text: Coastal zone [ENVO:00000486]

text: FWD (Golay167): TGTCAAAGTGAC; REV (BC162): GTTCGGTGTCCA

text: Powersoil DNA Isolation kit

text: not collected

text: plastisphere metagenome

text: not collected

text: not collected

characteristic: pcr primers

text: not collected

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

text: not collected

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

text: not collected

characteristic: total carbon (mg/L)

characteristic: total nitrogen (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not collected

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

text: not collected

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

text: PBSeT incubation - eulittoral habitat - 2.5 months

characteristic: description

text: not collected

characteristic: dissolved organic carbon (mg/L)

text: not collected

characteristic: dissolved oxygen (mg/L)

text: Field incubation, Time series

text: Mediterranean Sea

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Tyrrhenian Sea: Italy, Island of Elba coast

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay169): CTATGTATTAGT; REV (BC164): CTACCGATTGCG

characteristic: multiplex identifiers

text: not collected

characteristic: nitrate (mg/L)

text: not collected

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

text: not collected

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

text: not collected

characteristic: pH ()

text: not collected

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: not collected

text: not collected

text: Open-Bio bioplastics

text: not collected

characteristic: salinity (psu)

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: not collected

text: 16S/18S SSU rRNA

text: 400bp, 600bp

text: not collected

characteristic: temperature (ºC)

characteristic: title

text: not collected

text: not collected


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not collected

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

text: not collected

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

text: not collected

characteristic: dissolved organic carbon (mg/L)

text: not collected

characteristic: dissolved oxygen (mg/L)

characteristic: elevation

text: Marine sediment [ENVO:03000033]|Plastisphere [ENVO:06105106]

characteristic: environmental medium

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

text: Coastal zone [ENVO:00000486]

text: FWD (Golay171): ACTCCCGTGTGA; REV (BC166): GAGAGTCCACTT

text: not collected

text: not collected

text: Powersoil DNA Isolation kit

text: not collected

text: plastisphere metagenome

text: not collected

text: not collected

text: Initial denaturation: 98degC_30s; 30 cycles of denaturation: 98degC_10s, annealing: 50degC_15s, elongation: 72degC_30s; final elongation: 72degC_5min

characteristic: pcr primers

text: not collected

characteristic: phaeopigments (µg/L)

text: not collected

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

text: not collected

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

text: not collected

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

text: not collected

text: not collected

characteristic: total carbon (mg/L)

text: not collected

characteristic: total nitrogen (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not collected

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

text: not collected

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

text: not collected

characteristic: dissolved organic carbon (mg/L)

text: not collected

characteristic: dissolved oxygen (mg/L)

text: Field incubation, Time series

text: Mediterranean Sea

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay173): CGGTATAGCAAT; REV (BC168): CTAACCTCATAT

characteristic: multiplex identifiers

text: not collected

characteristic: nitrate (mg/L)

text: not collected

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

text: not collected

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

text: not collected

characteristic: pH ()

text: not collected

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: FWD (515F-Y): GTGYCAGCMGCCGCGGTAA; REV (926R): CCGYCAATTYMTTTRAGTTT

text: not collected

text: not collected

text: Open-Bio bioplastics

text: not collected

characteristic: salinity (psu)

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: not collected

text: 16S/18S SSU rRNA

text: 400bp, 600bp

text: not collected

characteristic: temperature (ºC)

characteristic: title

text: not collected

text: not collected


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not collected

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

text: not collected

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

text: PHB incubation - eulittoral habitat - 2.5 months

characteristic: description

text: not collected

characteristic: dissolved organic carbon (mg/L)

text: not collected

characteristic: dissolved oxygen (mg/L)

characteristic: elevation

characteristic: environmental medium

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

text: Coastal zone [ENVO:00000486]

text: FWD (Golay175): GACTCTGCTCAG; REV (BC170): AGCTTCGACAGT

text: not collected

text: not collected

text: Powersoil DNA Isolation kit

text: not collected

text: plastisphere metagenome

text: not collected

text: not collected

characteristic: pcr primers

text: not collected

characteristic: phaeopigments (µg/L)

text: not collected

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

text: not collected

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

text: not collected

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

text: not collected

text: not collected

characteristic: total carbon (mg/L)

text: not collected

characteristic: total nitrogen (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not collected

characteristic: ammonium (mg/L)

text: Marine benthic biome [ENVO:01000024]

characteristic: broad-scale environmental context

text: not collected

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

text: not collected

characteristic: dissolved organic carbon (mg/L)

text: not collected

characteristic: dissolved oxygen (mg/L)

text: Field incubation, Time series

text: Mediterranean Sea

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay177): GTCATGCTCCAG; REV (BC172): GAGAGGGATCAC

characteristic: multiplex identifiers

text: not collected

characteristic: nitrate (mg/L)

text: not collected

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

text: not collected

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

text: not collected

characteristic: pH ()

text: not collected

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: not collected

text: not collected

text: Open-Bio bioplastics

text: not collected

characteristic: salinity (psu)

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: not collected

text: 16S/18S SSU rRNA

text: 400bp, 600bp

text: not collected

characteristic: temperature (ºC)

characteristic: title

text: not collected

text: not collected