domain: self.PENELOPE HiC

status: PUBLIC

characteristic: SRA accession

text: mailto:laurence.liaubet@inrae.fr

characteristic: availability

characteristic: biosample id

unit: YYYY-MM-DD

characteristic: birth date

text: Centre INRAe GenESI, Magneraud, France

characteristic: birth location

unit: decimal degree

characteristic: birth location latitude

characteristic: birth location longitude

unit: grams

characteristic: birth weight

text: Large White

characteristic: breed

characteristic: child of

text: normal

characteristic: health status

text: organism

characteristic: material

text: Sus scrofa

characteristic: organism

unit: day

characteristic: pregnancy length

text: FAANG

characteristic: project

text: Fetus, 110 days gestational age, Large White

characteristic: sample description

characteristic: sample name

text: Female

characteristic: sex

text: Feto-maternal crosstalk associated to fetal maturity using an integrative omics approach (transcriptome, metabolome and lipidome) and questioning simultaneously the two adjacent tissues (placenta/endometrium). The biobank includes endometrium samples (maternal) supplying fetuses and their adjacent placenta (fetal) samples.

characteristic: submission description

characteristic: submission title

type: child of

ANR

Role: funder

Address: 50, avenue Daumesnil 75012 PARIS

INRAE

Role: investigator

Address:
24, Chemin de Borde Rouge, CS 52627
31326 CASTANET TOLOSAN cedex,
France

Agnès  Bonnet

FirstName: Agnès

LastName: Bonnet

Role: submitter

Laure Gress

FirstName: Laure

LastName: Gress

Role: experiment performer

Laurence Liaubet

FirstName: Laurence

LastName: Liaubet

Laurianne Canario

FirstName: Laurianne

LastName: Canario

Role: consultant

Nathalie Iannuccelli

FirstName: Nathalie

LastName: Iannuccelli

Yvon Billon

FirstName: Yvon

LastName: Billon

Role: biomaterial provider


characteristic: SRA accession

text: mailto:laurence.liaubet@inrae.fr

characteristic: availability

characteristic: biosample id

characteristic: birth date

text: Centre INRAe GenESI, Magneraud, France

text: Large White

text: normal

text: organism

text: Sus scrofa

text: FAANG

text: Male

type: derived from


text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

characteristic: birth location

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text: Large White

text: normal

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text: Male


text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

text: Large White

characteristic: breed

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text: Sus scrofa

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text: Female


text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

text: Pig crossbreed

text: normal

text: organism

text: Sus scrofa

characteristic: organism

characteristic: pregnancy length

text: FAANG

characteristic: project

text: Fetus, 110 days gestational age, Large WhitexMeishan

characteristic: sample description

text: Male


text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

text: Pig crossbreed

text: normal

text: organism

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text: FAANG

characteristic: sample name

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characteristic: sex

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characteristic: SRA accession

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characteristic: availability

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text: Centre INRAe GenESI, Magneraud, France

text: Pig crossbreed

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text: Sus scrofa

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text: Female


text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

characteristic: birth location

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text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

text: Pig crossbreed

characteristic: breed

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text: Sus scrofa

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text: Fetus, 110 days gestational age, MeishanxLarge White

text: Female


text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

text: Pig crossbreed

text: normal

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characteristic: project

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text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

text: Pig crossbreed

text: normal

text: organism

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characteristic: sample name

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characteristic: SRA accession

text: mailto:laurence.liaubet@inrae.fr

characteristic: availability

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text: Centre INRAe GenESI, Magneraud, France

text: Pig crossbreed

text: normal

text: organism

text: Sus scrofa

text: FAANG

text: Female


text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

characteristic: birth location

characteristic: birth location latitude

characteristic: birth location longitude

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text: Meishan

text: normal

text: organism

text: Sus scrofa

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text: Fetus, 110 days gestational age, Meishan

text: Female


text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

text: Meishan

characteristic: breed

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text: mailto:laurence.liaubet@inrae.fr

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text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

text: Large White

text: normal

text: organism

text: Sus scrofa

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text: Fetus, 90 days gestational age, Large White

characteristic: sample name

text: Female

characteristic: sex

characteristic: submission description

characteristic: submission title


characteristic: SRA accession

text: mailto:laurence.liaubet@inrae.fr

characteristic: availability

characteristic: biosample id

characteristic: birth date

text: Centre INRAe GenESI, Magneraud, France

text: Large White

text: normal

text: organism

text: Sus scrofa

text: FAANG

text: Male


text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

characteristic: birth location

characteristic: birth location latitude

characteristic: birth location longitude

characteristic: birth weight

text: Large White

text: normal

text: organism

text: Sus scrofa

text: FAANG

text: Female


text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

text: Large White

characteristic: breed

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text: FAANG

text: Male


text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

text: Pig crossbreed

text: normal

text: organism

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characteristic: organism

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text: FAANG

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characteristic: sample description

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text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

text: Pig crossbreed

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characteristic: sample name

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text: Centre INRAe GenESI, Magneraud, France

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text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

characteristic: birth location

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text: Pig crossbreed

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text: organism

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text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

text: Pig crossbreed

characteristic: breed

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text: normal

characteristic: health status

text: organism

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text: Sus scrofa

text: FAANG

text: Fetus, 90 days gestational age, MeishanxLarge White

text: Female


text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

text: Pig crossbreed

text: normal

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text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

text: Pig crossbreed

text: normal

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text: FAANG

characteristic: sample name

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characteristic: sex

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text: mailto:laurence.liaubet@inrae.fr

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text: Centre INRAe GenESI, Magneraud, France

text: Meishan

text: normal

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text: Fetus, 90 days gestational age, Meishan

text: Male


text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

characteristic: birth location

characteristic: birth location latitude

characteristic: birth location longitude

characteristic: birth weight

text: Meishan

text: normal

text: organism

text: Sus scrofa

text: FAANG

text: Fetus, 90 days gestational age, Meishan

text: Male


text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

text: Meishan

characteristic: breed

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text: Female


text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

text: Meishan

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text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

text: Large White

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characteristic: sample name

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text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

characteristic: birth location

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characteristic: birth location longitude

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text: Large White

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text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

text: Large White

characteristic: breed

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text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

text: Large White

text: normal

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text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

text: Large White

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text: mailto:laurence.liaubet@inrae.fr

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characteristic: birth location

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text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

text: Pig crossbreed

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text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

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text: mailto:laurence.liaubet@inrae.fr

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text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

characteristic: birth location

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text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

text: Pig crossbreed

characteristic: breed

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text: normal

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text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

text: Pig crossbreed

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text: mailto:laurence.liaubet@inrae.fr

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text: mailto:laurence.liaubet@inrae.fr

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characteristic: birth location

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text: mailto:laurence.liaubet@inrae.fr

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text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

text: Meishan

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text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

text: Large White

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text: FAANG

characteristic: sample name

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characteristic: sex

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text: Centre INRAe GenESI, Magneraud, France

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text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

characteristic: birth location

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text: Large White

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text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

text: Large White

characteristic: breed

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text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

text: Large White

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text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

text: Large White

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text: Centre INRAe GenESI, Magneraud, France

text: Pig crossbreed

text: normal

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text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

characteristic: birth location

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text: Pig crossbreed

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text: FAANG

text: Female


text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

text: Pig crossbreed

characteristic: breed

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text: normal

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text: Sus scrofa

text: FAANG

text: Male


text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

text: Pig crossbreed

text: normal

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text: Sus scrofa

characteristic: organism

characteristic: pregnancy length

text: FAANG

characteristic: project

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text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

text: Pig crossbreed

text: normal

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text: Sus scrofa

text: FAANG

characteristic: sample name

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text: mailto:laurence.liaubet@inrae.fr

characteristic: availability

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text: Centre INRAe GenESI, Magneraud, France

text: Pig crossbreed

text: normal

text: organism

text: Sus scrofa

text: FAANG

text: Male


text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

characteristic: birth location

characteristic: birth location latitude

characteristic: birth location longitude

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text: Pig crossbreed

text: normal

text: organism

text: Sus scrofa

text: FAANG

text: Female


text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

text: Pig crossbreed

characteristic: breed

characteristic: child of

text: normal

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text: organism

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text: Sus scrofa

text: FAANG

text: Male


text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

text: Pig crossbreed

text: normal

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text: Sus scrofa

characteristic: organism

characteristic: pregnancy length

text: FAANG

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text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

text: Pig crossbreed

text: normal

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text: Sus scrofa

text: FAANG

characteristic: sample name

text: Male

characteristic: sex

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text: mailto:laurence.liaubet@inrae.fr

characteristic: availability

characteristic: biosample id

characteristic: birth date

text: Centre INRAe GenESI, Magneraud, France

text: Meishan

text: normal

text: organism

text: Sus scrofa

text: FAANG

text: Fetus, 90 days gestational age, Meishan

text: Male


text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

characteristic: birth location

characteristic: birth location latitude

characteristic: birth location longitude

characteristic: birth weight

text: Meishan

text: normal

text: organism

text: Sus scrofa

text: FAANG

text: Fetus, 90 days gestational age, Meishan

text: Female


text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

text: Meishan

characteristic: breed

characteristic: child of

text: normal

characteristic: health status

text: organism

characteristic: material

text: Sus scrofa

text: FAANG

text: Fetus, 90 days gestational age, Meishan

text: Female


text: mailto:laurence.liaubet@inrae.fr

text: Centre INRAe GenESI, Magneraud, France

text: Meishan

text: normal

text: organism

text: Sus scrofa

characteristic: organism

characteristic: pregnancy length

text: FAANG

characteristic: project

text: Fetus, 90 days gestational age, Meishan

characteristic: sample description

text: Male


characteristic: ENA first public

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

tag: Namespace:Max Planck Unit for the Science of Pathogens

characteristic: Submitter Id

characteristic: collection date

text: C medium

text: Overnight cultures of S. pyogenes SF370 were diluted to an OD620 of 0.02 in C medium and grown at 37 degree C and 5% CO2. At early logarithmic growth phase (EL; OD620 of 0.1), an aliquot of the culture was removed and mixed with an equal volume of ice-cold acetone:ethanol (1:1, v/v). Cells were pelleted, washed with TE-sucrose (50 mM Tris-HCl, 10 mM EDTA, 50 mM NaCl, 25% sucrose; pH 8.0) and stored at -80 degree C.

characteristic: description

text: wild type

characteristic: genotype

text: Germany

characteristic: geographic location (country and/or sea)

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

characteristic: serotype (required for a seropositive sample)

characteristic: strain

text: S. pyogenes SF370, early logarithmic growth phase in C medium, replicate 1

characteristic: title


text: Max Planck Unit for the Science of Pathogens

text: public

characteristic: SRA accession

text: C medium

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

characteristic: organism

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, early logarithmic growth phase in C medium, replicate 2


text: public

text: C medium

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, early logarithmic growth phase in C medium, replicate 3


text: public

text: C medium

text: Overnight cultures of S. pyogenes SF370 were diluted to an OD620 of 0.02 in C medium and grown at 37 degree C and 5% CO2. At mid-logarithmic growth phase (ML; OD620 of 0.25), an aliquot of the culture was removed and mixed with an equal volume of ice-cold acetone:ethanol (1:1, v/v). Cells were pelleted, washed with TE-sucrose (50 mM Tris-HCl, 10 mM EDTA, 50 mM NaCl, 25% sucrose; pH 8.0) and stored at -80 degree C.

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, mid-logarithmic growth phase in C medium, replicate 1


text: public

text: C medium

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, mid-logarithmic growth phase in C medium, replicate 2


text: public

text: C medium

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, mid-logarithmic growth phase in C medium, replicate 3


text: public

text: C medium

text: Overnight cultures of S. pyogenes SF370 were diluted to an OD620 of 0.02 in C medium and grown at 37 degree C and 5% CO2. At early stationary growth phase (ES; OD620 of 0.4), an aliquot of the culture was removed and mixed with an equal volume of ice-cold acetone:ethanol (1:1, v/v). Cells were pelleted, washed with TE-sucrose (50 mM Tris-HCl, 10 mM EDTA, 50 mM NaCl, 25% sucrose; pH 8.0) and stored at -80 degree C.

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, early stationary growth phase in C medium, replicate 1


text: public

text: C medium

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, early stationary growth phase in C medium, replicate 2


text: public

text: C medium

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, early stationary growth phase in C medium, replicate 3


text: public

text: C medium

text: S. pyogenes SF370 was transformed with the empty vector control pEC2812, and transformants were selected on TSA plates with 3% sheep blood and 300 �g/mL kanamycin. Overnight cultures in C medium with 300 �g/mL kanamycin were inoculated with single colonies, grown overnight, and diluted to an OD620 of 0.02. Cultures were grown at 37 degree C and 5% CO2, and culture aliquots were mixed with an equal volume of ice-cold acetone:ethanol (1:1, v/v) at mid-logarithmic growth phase (ML; OD620 of 0.25). Cells were pelleted, washed with TE-sucrose (50 mM Tris-HCl, 10 mM EDTA, 50 mM NaCl, 25% sucrose; pH 8.0) and stored at -80 degree C.

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370 with empty vector pEC2812, mid-logarithmic growth phase in C medium, replicate 1


text: public

text: C medium

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370 with empty vector pEC2812, mid-logarithmic growth phase in C medium, replicate 2


text: public

text: C medium

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370 with empty vector pEC2812, mid-logarithmic growth phase in C medium, replicate 3


text: public

text: C medium

text: S. pyogenes SF370 was transformed with the tadA overexpression plasmid pEC2813, and transformants were selected on TSA plates with 3% sheep blood and 300 �g/mL kanamycin. Overnight cultures in C medium with 300 �g/mL kanamycin were inoculated with single colonies, grown overnight, and diluted to an OD620 of 0.02. Cultures were grown at 37 degree C and 5% CO2, and culture aliquots were mixed with an equal volume of ice-cold acetone:ethanol (1:1, v/v) at mid-logarithmic growth phase (ML; OD620 of 0.25). Cells were pelleted, washed with TE-sucrose (50 mM Tris-HCl, 10 mM EDTA, 50 mM NaCl, 25% sucrose; pH 8.0) and stored at -80 degree C.

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370 with tadA-overexpressing pEC2813, mid-logarithmic growth phase in C medium, replicate 1


text: Max Planck Unit for the Science of Pathogens

text: public

text: C medium

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370 with tadA-overexpressing pEC2813, mid-logarithmic growth phase in C medium, replicate 2


text: public

text: C medium

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370 with tadA-overexpressing pEC2813, mid-logarithmic growth phase in C medium, replicate 3


text: public

text: C medium

text: Overnight cultures of S. pyogenes 5448 were diluted to an OD620 of 0.02 in C medium and grown at 37 degree C and 5% CO2. At early logarithmic growth phase (EL; OD620 of 0.1), an aliquot of the culture was removed and mixed with an equal volume of ice-cold acetone:ethanol (1:1, v/v). Cells were pelleted, washed with TE-sucrose (50 mM Tris-HCl, 10 mM EDTA, 50 mM NaCl, 25% sucrose; pH 8.0) and stored at -80 degree C.

text: wild type

text: Germany

text: Streptococcus pyogenes

text: Streptococcus pyogenes

text: S. pyogenes 5448, early logarithmic growth phase in C medium, replicate 1


text: public

text: C medium

text: wild type

text: Germany

text: Streptococcus pyogenes

text: Streptococcus pyogenes

text: S. pyogenes 5448, early logarithmic growth phase in C medium, replicate 2


text: public

text: C medium

text: Overnight cultures of S. pyogenes 5448 were diluted to an OD620 of 0.02 in C medium and grown at 37 degree C and 5% CO2. At mid-logarithmic growth phase (ML; OD620 of 0.3), an aliquot of the culture was removed and mixed with an equal volume of ice-cold acetone:ethanol (1:1, v/v). Cells were pelleted, washed with TE-sucrose (50 mM Tris-HCl, 10 mM EDTA, 50 mM NaCl, 25% sucrose; pH 8.0) and stored at -80 degree C.

text: wild type

text: Germany

text: Streptococcus pyogenes

text: Streptococcus pyogenes

text: S. pyogenes 5448, mid-logarithmic growth phase in C medium, replicate 1


text: public

text: C medium

text: wild type

text: Germany

text: Streptococcus pyogenes

text: Streptococcus pyogenes

text: S. pyogenes 5448, mid-logarithmic growth phase in C medium, replicate 2


text: public

text: C medium

text: Overnight cultures of S. pyogenes 5448 were diluted to an OD620 of 0.02 in C medium and grown at 37 degree C and 5% CO2. At early stationary growth phase (ES; OD620 of 0.45), an aliquot of the culture was removed and mixed with an equal volume of ice-cold acetone:ethanol (1:1, v/v). Cells were pelleted, washed with TE-sucrose (50 mM Tris-HCl, 10 mM EDTA, 50 mM NaCl, 25% sucrose; pH 8.0) and stored at -80 degree C.

text: wild type

text: Germany

text: Streptococcus pyogenes

text: Streptococcus pyogenes

text: S. pyogenes 5448, early stationary growth phase in C medium, replicate 1


text: public

text: C medium

text: wild type

text: Germany

text: Streptococcus pyogenes

text: Streptococcus pyogenes

text: S. pyogenes 5448, early stationary growth phase in C medium, replicate 2


text: public

text: C medium

text: Overnight cultures of S. pyogenes 5448AP were diluted to an OD620 of 0.02 in C medium and grown at 37 degree C and 5% CO2. At early logarithmic growth phase (EL; OD620 of 0.1), an aliquot of the culture was removed and mixed with an equal volume of ice-cold acetone:ethanol (1:1, v/v). Cells were pelleted, washed with TE-sucrose (50 mM Tris-HCl, 10 mM EDTA, 50 mM NaCl, 25% sucrose; pH 8.0) and stored at -80 degree C.

text: covS(-)

text: Germany

text: Streptococcus pyogenes

text: Streptococcus pyogenes

text: S. pyogenes 5448AP, early logarithmic growth phase in C medium, replicate 1


text: public

text: C medium

text: covS(-)

text: Germany

text: Streptococcus pyogenes

text: Streptococcus pyogenes

text: S. pyogenes 5448AP, early logarithmic growth phase in C medium, replicate 2


text: public

text: C medium

text: Overnight cultures of S. pyogenes 5448AP were diluted to an OD620 of 0.02 in C medium and grown at 37 degree C and 5% CO2. At mid-logarithmic growth phase (ML; OD620 of 0.3), an aliquot of the culture was removed and mixed with an equal volume of ice-cold acetone:ethanol (1:1, v/v). Cells were pelleted, washed with TE-sucrose (50 mM Tris-HCl, 10 mM EDTA, 50 mM NaCl, 25% sucrose; pH 8.0) and stored at -80 degree C.

text: covS(-)

text: Germany

text: Streptococcus pyogenes

text: Streptococcus pyogenes

text: S. pyogenes 5448AP, mid-logarithmic growth phase in C medium, replicate 1


text: Max Planck Unit for the Science of Pathogens

text: public

text: C medium

text: covS(-)

text: Germany

text: Streptococcus pyogenes

text: Streptococcus pyogenes

text: S. pyogenes 5448AP, mid-logarithmic growth phase in C medium, replicate 2


text: public

text: C medium

text: Overnight cultures of S. pyogenes 5448AP were diluted to an OD620 of 0.02 in C medium and grown at 37 degree C and 5% CO2. At early stationary growth phase (ES; OD620 of 0.45), an aliquot of the culture was removed and mixed with an equal volume of ice-cold acetone:ethanol (1:1, v/v). Cells were pelleted, washed with TE-sucrose (50 mM Tris-HCl, 10 mM EDTA, 50 mM NaCl, 25% sucrose; pH 8.0) and stored at -80 degree C.

text: covS(-)

text: Germany

text: Streptococcus pyogenes

text: Streptococcus pyogenes

text: S. pyogenes 5448AP, early stationary growth phase in C medium, replicate 1


text: public

text: C medium

text: covS(-)

text: Germany

text: Streptococcus pyogenes

text: Streptococcus pyogenes

text: S. pyogenes 5448AP, early stationary growth phase in C medium, replicate 2


text: public

text: C medium

text: Overnight cultures of S. pyogenes SF370 in C medium were pelleted, washed once with 1x PBS and used to inoculate C medium at an OD620 of 0.02. Cultures were grown at 37 degree C and 5% CO2 until mid-logarithmic growth phase (ML; OD620 of 0.25), and an aliquot of the culture was removed and mixed with an equal volume of ice-cold acetone:ethanol (1:1, v/v). Cells were pelleted, washed with TE-sucrose (50 mM Tris-HCl, 10 mM EDTA, 50 mM NaCl, 25% sucrose; pH 8.0) and stored at -80 degree C.

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, mid-logarithmic growth phase in C medium, replicate 1


text: public

text: C medium

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, mid-logarithmic growth phase in C medium, replicate 2


text: public

text: C medium

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, mid-logarithmic growth phase in C medium, replicate 3


text: public

text: Chemically Defined Medium

text: Overnight cultures of S. pyogenes SF370 in C medium were pelleted, washed once with 1x PBS and used to inoculate chemically defined medium (CDM) at an OD620 of 0.02. Cultures were grown at 37 degree C and 5% CO2 until mid-logarithmic growth phase (ML; OD620 of 0.22), and an aliquot of the culture was removed and mixed with an equal volume of ice-cold acetone:ethanol (1:1, v/v). Cells were pelleted, washed with TE-sucrose (50 mM Tris-HCl, 10 mM EDTA, 50 mM NaCl, 25% sucrose; pH 8.0) and stored at -80 degree C.

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, mid-logarithmic growth phase in CDM, replicate 1


text: public

text: Chemically Defined Medium

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, mid-logarithmic growth phase in CDM, replicate 2


text: public

text: Chemically Defined Medium

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, mid-logarithmic growth phase in CDM, replicate 3


text: public

text: THY

text: Overnight cultures of S. pyogenes SF370 in C medium were pelleted, washed once with 1x PBS and used to inoculate THY at an OD620 of 0.02. Cultures were grown at 37 degree C and 5% CO2 until mid-logarithmic growth phase (ML; OD620 of 0.25), and an aliquot of the culture was removed and mixed with an equal volume of ice-cold acetone:ethanol (1:1, v/v). Cells were pelleted, washed with TE-sucrose (50 mM Tris-HCl, 10 mM EDTA, 50 mM NaCl, 25% sucrose; pH 8.0) and stored at -80 degree C.

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, mid-logarithmic growth phase in THY, replicate 1


text: public

text: THY

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, mid-logarithmic growth phase in THY, replicate 2


text: public

text: THY

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, mid-logarithmic growth phase in THY, replicate 3


text: Max Planck Unit for the Science of Pathogens

text: public

text: C medium

text: Overnight cultures of S. pyogenes SF370 were diluted to an OD620 of 0.02 in C medium and grown at 37 degree C and 5% CO2. At mid-logarithmic growth phase (ML; OD620 of 0.25), an aliquot of the main culture was removed and mixed with an equal volume of ice-cold acetone:ethanol (1:1, v/v). Cells were pelleted, washed with TE-sucrose (50 mM Tris-HCl, 10 mM EDTA, 50 mM NaCl, 25% sucrose; pH 8.0) and stored at -80 degree C. The remaining main culture was splitted into three sub-cultures and treated with different concentrations of H2O2 for different amounts of time.

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, before H2O2 challenge, mid-logarithmic growth phase in C medium, replicate 1


text: public

text: C medium

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, before H2O2 challenge, mid-logarithmic growth phase in C medium, replicate 2


text: public

text: C medium

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, before H2O2 challenge, mid-logarithmic growth phase in C medium, replicate 3


text: public

text: C medium

text: Overnight cultures of S. pyogenes SF370 were diluted to an OD620 of 0.02 in C medium and grown at 37 degree C and 5% CO2. At mid-logarithmic growth phase (ML; OD620 of 0.25), the main culture was splitted into three sub-cultures, and this culture aliquot was mock-treated with water. After 15 min, an aliquot of the mock-treated sub-culture was removed and mixed with an equal volume of ice-cold acetone:ethanol (1:1, v/v). Cells were pelleted, washed with TE-sucrose (50 mM Tris-HCl, 10 mM EDTA, 50 mM NaCl, 25% sucrose; pH 8.0) and stored at -80 degree C.

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, mock-treated with water instead of H2O2 for 15 min, C medium, replicate 1


text: public

text: C medium

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, mock-treated with water instead of H2O2 for 15 min, C medium, replicate 2


text: public

text: C medium

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, mock-treated with water instead of H2O2 for 15 min, C medium, replicate 3


text: public

text: C medium

text: Overnight cultures of S. pyogenes SF370 were diluted to an OD620 of 0.02 in C medium and grown at 37 degree C and 5% CO2. At mid-logarithmic growth phase (ML; OD620 of 0.25), the main culture was splitted into three sub-cultures, and this culture aliquot was treated with 0.5 mM H2O2. After 15 min, an aliquot of the 0.5 mM H2O2-treated sub-culture was removed and mixed with an equal volume of ice-cold acetone:ethanol (1:1, v/v). Cells were pelleted, washed with TE-sucrose (50 mM Tris-HCl, 10 mM EDTA, 50 mM NaCl, 25% sucrose; pH 8.0) and stored at -80 degree C.

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, treated with 0.5 mM H2O2 for 15 min, C medium, replicate 1


text: public

text: C medium

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, treated with 0.5 mM H2O2 for 15 min, C medium, replicate 2


text: public

text: C medium

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, treated with 0.5 mM H2O2 for 15 min, C medium, replicate 3


text: public

text: C medium

text: Overnight cultures of S. pyogenes SF370 were diluted to an OD620 of 0.02 in C medium and grown at 37 degree C and 5% CO2. At mid-logarithmic growth phase (ML; OD620 of 0.25), the main culture was splitted into three sub-cultures, and this culture aliquot was treated with 1.0 mM H2O2. After 15 min, an aliquot of the 1.0 mM H2O2-treated sub-culture was removed and mixed with an equal volume of ice-cold acetone:ethanol (1:1, v/v). Cells were pelleted, washed with TE-sucrose (50 mM Tris-HCl, 10 mM EDTA, 50 mM NaCl, 25% sucrose; pH 8.0) and stored at -80 degree C.

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, treated with 1.0 mM H2O2 for 15 min, C medium, replicate 1


text: public

text: C medium

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, treated with 1.0 mM H2O2 for 15 min, C medium, replicate 2


text: public

text: C medium

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, treated with 1.0 mM H2O2 for 15 min, C medium, replicate 3


text: Max Planck Unit for the Science of Pathogens

text: public

text: C medium

text: Overnight cultures of S. pyogenes SF370 were diluted to an OD620 of 0.02 in C medium and grown at 37 degree C and 5% CO2. At mid-logarithmic growth phase (ML; OD620 of 0.25), the main culture was splitted into three sub-cultures, and this culture aliquot was mock-treated with water. After 30 min, an aliquot of the mock-treated sub-culture was removed and mixed with an equal volume of ice-cold acetone:ethanol (1:1, v/v). Cells were pelleted, washed with TE-sucrose (50 mM Tris-HCl, 10 mM EDTA, 50 mM NaCl, 25% sucrose; pH 8.0) and stored at -80 degree C.

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, mock-treated with water instead of H2O2 for 30 min, C medium, replicate 1


text: public

text: C medium

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, mock-treated with water instead of H2O2 for 30 min, C medium, replicate 2


text: public

text: C medium

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, mock-treated with water instead of H2O2 for 30 min, C medium, replicate 3


text: public

text: C medium

text: Overnight cultures of S. pyogenes SF370 were diluted to an OD620 of 0.02 in C medium and grown at 37 degree C and 5% CO2. At mid-logarithmic growth phase (ML; OD620 of 0.25), the main culture was splitted into three sub-cultures, and this culture aliquot was treated with 0.5 mM H2O2. After 30 min, an aliquot of the 0.5 mM H2O2-treated sub-culture was removed and mixed with an equal volume of ice-cold acetone:ethanol (1:1, v/v). Cells were pelleted, washed with TE-sucrose (50 mM Tris-HCl, 10 mM EDTA, 50 mM NaCl, 25% sucrose; pH 8.0) and stored at -80 degree C.

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, treated with 0.5 mM H2O2 for 30 min, C medium, replicate 1


text: public

text: C medium

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, treated with 0.5 mM H2O2 for 30 min, C medium, replicate 2


text: public

text: C medium

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, treated with 0.5 mM H2O2 for 30 min, C medium, replicate 3


text: public

text: C medium

text: Overnight cultures of S. pyogenes SF370 were diluted to an OD620 of 0.02 in C medium and grown at 37 degree C and 5% CO2. At mid-logarithmic growth phase (ML; OD620 of 0.25), the main culture was splitted into three sub-cultures, and this culture aliquot was treated with 1.0 mM H2O2. After 30 min, an aliquot of the 1.0 mM H2O2-treated sub-culture was removed and mixed with an equal volume of ice-cold acetone:ethanol (1:1, v/v). Cells were pelleted, washed with TE-sucrose (50 mM Tris-HCl, 10 mM EDTA, 50 mM NaCl, 25% sucrose; pH 8.0) and stored at -80 degree C.

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, treated with 1.0 mM H2O2 for 30 min, C medium, replicate 1


text: public

text: C medium

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, treated with 1.0 mM H2O2 for 30 min, C medium, replicate 2


text: public

text: C medium

text: wild type

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370, treated with 1.0 mM H2O2 for 30 min, C medium, replicate 3


text: public

text: THY

text: Overnight cultures of S. pyogenes SF370 were diluted 1/200 in THY and grown without anhydrotetracycline (AHT) at 37 degree C and 5% CO2. At mid-logarithmic growth phase (ML; OD620 of 0.25), an aliquot of the culture was removed and mixed with an equal volume of ice-cold acetone:ethanol (1:1, v/v). Cells were pelleted, washed with TE-sucrose (50 mM Tris-HCl, 10 mM EDTA, 50 mM NaCl, 25% sucrose; pH 8.0) and stored at -80 degree C.

text: Ptet-rnjA

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370 Ptet-rnjA, -AHT, mid-logarithmic growth phase in THY, replicate 1


text: public

text: THY

text: Ptet-rnjA

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370 Ptet-rnjA, -AHT, mid-logarithmic growth phase in THY, replicate 2


text: public

text: THY

text: Ptet-rnjA

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370 Ptet-rnjA, -AHT, mid-logarithmic growth phase in THY, replicate 3


text: Max Planck Unit for the Science of Pathogens

text: public

text: THY

text: Overnight cultures of S. pyogenes SF370 were diluted 1/200 in THY and grown with 0.1 ng/mL anhydrotetracycline (AHT) at 37 degree C and 5% CO2. At mid-logarithmic growth phase (ML; OD620 of 0.25), an aliquot of the culture was removed and mixed with an equal volume of ice-cold acetone:ethanol (1:1, v/v). Cells were pelleted, washed with TE-sucrose (50 mM Tris-HCl, 10 mM EDTA, 50 mM NaCl, 25% sucrose; pH 8.0) and stored at -80 degree C.

text: Ptet-rnjA

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370 Ptet-rnjA, +AHT, mid-logarithmic growth phase in THY, replicate 1


text: public

text: THY

text: Ptet-rnjA

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370 Ptet-rnjA, +AHT, mid-logarithmic growth phase in THY, replicate 2


text: public

text: THY

text: Ptet-rnjA

text: Germany

text: Streptococcus pyogenes M1 GAS

text: Streptococcus pyogenes M1 GAS

text: S. pyogenes SF370 Ptet-rnjA, +AHT, mid-logarithmic growth phase in THY, replicate 3


text: public

tag: Namespace:London School of Hygiene and Tropical Medicine

text: WGS data of Anopheles darlingi collected in Candeias dos Jamari

text: Brazil

text: Anopheles darlingi

text: Anopheles darlingi


text: London School of Hygiene and Tropical Medicine

text: public

text: Brazil

text: Anopheles darlingi

text: Anopheles darlingi


text: public

text: WGS data of Anopheles darlingi colony isolate

text: Brazil

text: Anopheles darlingi

text: Anopheles darlingi


text: Royal NIOZ

text: public

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

unit: m

characteristic: depth

text: PBSeT incubation - pelagic habitat - 2.5 months

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

text: Sea Water [ENVO:00002149]|Plastisphere [ENVO:06105106]

characteristic: environmental medium

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Tyrrhenian Sea: Italy, Island of Pianosa, National Park Tuscan Archipelago

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay1): AGCCTTCGTCGC; REV (BC252): CTCTTCTGATCA

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

text: not collected

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: pH

text: not collected

characteristic: particulate organic carbon (mg/L)

text: Initial denaturation: 98degC_30s; 30 cycles of denaturation: 98degC_10s, annealing: 50degC_15s, elongation: 72degC_30s; final elongation: 72degC_5min

characteristic: pcr conditions

text: FWD (515F-Y): GTGYCAGCMGCCGCGGTAA; REV (926R): CCGYCAATTYMTTTRAGTTT

characteristic: pcr primers

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

unit: psu

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

unit: ºC

characteristic: temperature

characteristic: total depth of water column

text: not collected

characteristic: total inorganic nitrogen (mg/L)

characteristic: total nitrogen (mg/L)

characteristic: total phosphorus (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: environmental medium

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay3): TCCATACCGGAA; REV (BC254): CGCGGCGCAGCT

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

text: not collected

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

text: not collected

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title

text: not collected


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

text: Coastal zone [ENVO:00000486]

text: FWD (Golay5): AGCCCTGCTACA; REV (BC256): TGGACTCAGCTA

text: Powersoil DNA Isolation kit

text: not collected

text: plastisphere metagenome

text: not collected

characteristic: pcr primers

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

characteristic: temperature

characteristic: total depth of water column

text: not collected

characteristic: total inorganic nitrogen (mg/L)

characteristic: total nitrogen (mg/L)

characteristic: total phosphorus (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

text: PHB incubation - pelagic habitat - 2.5 months

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: environmental medium

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay7): CCTAACGGTCCA; REV (BC2): CTTCCAACTCAT

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

text: not collected

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

text: not collected

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title

text: not collected


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

text: Coastal zone [ENVO:00000486]

text: FWD (Golay9): CGCGCCTTAAAC; REV (BC4): GCCTAGCCCAAT

text: Powersoil DNA Isolation kit

text: not collected

text: plastisphere metagenome

text: not collected

characteristic: pcr primers

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

characteristic: temperature

characteristic: total depth of water column

text: not collected

characteristic: total inorganic nitrogen (mg/L)

characteristic: total nitrogen (mg/L)

characteristic: total phosphorus (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: environmental medium

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay11): TATGGTACCCAG; REV (BC6): GTTAGGGAGCGA

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

text: not collected

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

text: not collected

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title

text: not collected


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

text: LDPE incubation - pelagic habitat - 2.5 months

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

text: Coastal zone [ENVO:00000486]

text: FWD (Golay13): TACAATATCTGT; REV (BC8): TATACCGCTGCG

text: Powersoil DNA Isolation kit

text: not collected

text: plastisphere metagenome

text: not collected

characteristic: pcr primers

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

characteristic: temperature

characteristic: total depth of water column

text: not collected

characteristic: total inorganic nitrogen (mg/L)

characteristic: total nitrogen (mg/L)

characteristic: total phosphorus (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: environmental medium

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay15): AATTTAGGTAGG; REV (BC10): CTTACACTGCTT

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

text: not collected

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

text: not collected

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title

text: not collected


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

text: Coastal zone [ENVO:00000486]

text: FWD (Golay17): GACTCAACCAGT; REV (BC12): CCTGCACCTGCA

text: Powersoil DNA Isolation kit

text: not collected

text: plastisphere metagenome

text: not collected

characteristic: pcr primers

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

characteristic: temperature

characteristic: total depth of water column

text: not collected

characteristic: total inorganic nitrogen (mg/L)

characteristic: total nitrogen (mg/L)

characteristic: total phosphorus (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

text: PBSeT incubation - pelagic habitat - 5 months

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: environmental medium

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay19): GCCTCTACGTCG; REV (BC14): AGGACAAACTAT

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

text: not collected

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

text: not collected

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title

text: not collected


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

text: Sea Water [ENVO:00002149]|Plastisphere [ENVO:06105106]

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

text: Coastal zone [ENVO:00000486]

text: FWD (Golay21): ACTACTGAGGAT; REV (BC16): ATAACGGTGTAC

text: Powersoil DNA Isolation kit

text: not collected

text: plastisphere metagenome

text: not collected

characteristic: pcr primers

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

characteristic: temperature

characteristic: total depth of water column

text: not collected

characteristic: total inorganic nitrogen (mg/L)

characteristic: total nitrogen (mg/L)

characteristic: total phosphorus (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: environmental medium

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Tyrrhenian Sea: Italy, Island of Pianosa, National Park Tuscan Archipelago

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay23): AATTCACCTCCT; REV (BC18): GTCGTTACCCGC

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

text: not collected

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

text: not collected

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title

text: not collected


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

text: PHB incubation - pelagic habitat - 5 months

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

text: Coastal zone [ENVO:00000486]

text: FWD (Golay25): CGTATAAATGCG; REV (BC20): CGCAAGCCCGCG

text: Powersoil DNA Isolation kit

text: not collected

text: plastisphere metagenome

text: not collected

text: Initial denaturation: 98degC_30s; 30 cycles of denaturation: 98degC_10s, annealing: 50degC_15s, elongation: 72degC_30s; final elongation: 72degC_5min

text: FWD (515F-Y): GTGYCAGCMGCCGCGGTAA; REV (926R): CCGYCAATTYMTTTRAGTTT

characteristic: pcr primers

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

characteristic: temperature

characteristic: total depth of water column

text: not collected

characteristic: total inorganic nitrogen (mg/L)

characteristic: total nitrogen (mg/L)

characteristic: total phosphorus (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: environmental medium

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay27): ATGCTGCAACAC; REV (BC22): CGGTCAATTGAC

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

text: not collected

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

text: not collected

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title

text: not collected


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

text: Coastal zone [ENVO:00000486]

text: FWD (Golay29): ACTCGCTCGCTG; REV (BC24): ATGTTCCTCATC

text: Powersoil DNA Isolation kit

text: not collected

text: plastisphere metagenome

text: not collected

characteristic: pcr primers

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

characteristic: temperature

characteristic: total depth of water column

text: not collected

characteristic: total inorganic nitrogen (mg/L)

characteristic: total nitrogen (mg/L)

characteristic: total phosphorus (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

text: LDPE incubation - pelagic habitat - 5 months

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: environmental medium

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay31): TTCCTTAGTAGT; REV (BC26): CGACCTCGCATA

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

text: not collected

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

text: not collected

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title

text: not collected


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

text: Coastal zone [ENVO:00000486]

text: FWD (Golay33): CGTCCGTATGAA; REV (BC28): CATTCGTGGCGT

text: Powersoil DNA Isolation kit

text: not collected

text: plastisphere metagenome

text: not collected

characteristic: pcr primers

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

characteristic: temperature

characteristic: total depth of water column

text: not collected

characteristic: total inorganic nitrogen (mg/L)

characteristic: total nitrogen (mg/L)

characteristic: total phosphorus (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: environmental medium

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay35): ACGTGAGGAACG; REV (BC30): AGTACGCAGTCT

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

text: not collected

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

text: not collected

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title

text: not collected


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

text: PBSeT incubation - pelagic habitat - 7.5 months

characteristic: description

text: not collected

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

text: Coastal zone [ENVO:00000486]

text: FWD (Golay37): GGTTGCCCTGTA; REV (BC32): CAGTGCACGTCT

text: Powersoil DNA Isolation kit

text: plastisphere metagenome

text: not collected

characteristic: pcr primers

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

characteristic: temperature

characteristic: total depth of water column

text: not collected

characteristic: total inorganic nitrogen (mg/L)

characteristic: total nitrogen (mg/L)

characteristic: total phosphorus (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

text: not collected

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: environmental medium

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay39): CATATAGCCCGA; REV (BC34): ACTCACAGGAAT

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

text: not collected

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title

text: not collected


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

text: not collected

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

text: Sea Water [ENVO:00002149]|Plastisphere [ENVO:06105106]

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

text: Coastal zone [ENVO:00000486]

text: FWD (Golay41): GCCTATGAGATC; REV (BC36): TGGTGTTTATAT

text: Powersoil DNA Isolation kit

text: plastisphere metagenome

text: not collected

characteristic: pcr primers

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

characteristic: temperature

characteristic: total depth of water column

text: not collected

characteristic: total inorganic nitrogen (mg/L)

characteristic: total nitrogen (mg/L)

characteristic: total phosphorus (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

text: PHB incubation - pelagic habitat - 7.5 months

characteristic: description

text: not collected

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: environmental medium

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Tyrrhenian Sea: Italy, Island of Pianosa, National Park Tuscan Archipelago

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay43): CAAGTGAAGGGA; REV (BC38): TCTTCCTAAAGT

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

text: not collected

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title

text: not collected


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

text: not collected

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

text: Coastal zone [ENVO:00000486]

text: FWD (Golay45): CACGTTTATTCC; REV (BC40): GCCCTTCCCGTG

text: Powersoil DNA Isolation kit

text: plastisphere metagenome

text: not collected

characteristic: pcr primers

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

characteristic: temperature

characteristic: total depth of water column

text: not collected

characteristic: total inorganic nitrogen (mg/L)

characteristic: total nitrogen (mg/L)

characteristic: total phosphorus (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

text: not collected

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: environmental medium

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay47): TAATCGGTGCCA; REV (BC42): AGTATATGTTTC

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

text: not collected

characteristic: particulate organic carbon (mg/L)

text: Initial denaturation: 98degC_30s; 30 cycles of denaturation: 98degC_10s, annealing: 50degC_15s, elongation: 72degC_30s; final elongation: 72degC_5min

characteristic: pcr conditions

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title

text: not collected


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

text: LDPE incubation - pelagic habitat - 7.5 months

characteristic: description

text: not collected

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

text: Coastal zone [ENVO:00000486]

text: FWD (Golay49): TGACTAATGGCC; REV (BC44): AGAGCGGAACAA

text: Powersoil DNA Isolation kit

text: plastisphere metagenome

text: not collected

text: FWD (515F-Y): GTGYCAGCMGCCGCGGTAA; REV (926R): CCGYCAATTYMTTTRAGTTT

characteristic: pcr primers

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

characteristic: temperature

characteristic: total depth of water column

text: not collected

characteristic: total inorganic nitrogen (mg/L)

characteristic: total nitrogen (mg/L)

characteristic: total phosphorus (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

text: not collected

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: environmental medium

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay51): CGGGACACCCGA; REV (BC46): TCCGCTGCTGAC

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

text: not collected

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title

text: not collected


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

text: not collected

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

text: Coastal zone [ENVO:00000486]

text: FWD (Golay53): CTGTCTATACTA; REV (BC48): TCCGTGGTATAG

text: Powersoil DNA Isolation kit

text: plastisphere metagenome

text: not collected

characteristic: pcr primers

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

characteristic: temperature

characteristic: total depth of water column

text: not collected

characteristic: total inorganic nitrogen (mg/L)

characteristic: total nitrogen (mg/L)

characteristic: total phosphorus (mg/L)


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

text: PBSeT incubation - pelagic habitat - 10 months

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: environmental medium

text: Field incubation, Time series

characteristic: experimental factor

text: Mediterranean Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Coastal zone [ENVO:00000486]

characteristic: local environmental context

text: FWD (Golay55): TATGCCAGAGAT; REV (BC50): GTCAGAGTATTG

characteristic: multiplex identifiers

characteristic: nitrate (mg/L)

characteristic: nitrite (mg/L)

text: Powersoil DNA Isolation kit

characteristic: nucleic acid extraction

characteristic: organic carbon (mg/L)

text: plastisphere metagenome

characteristic: organism

characteristic: pH

text: not collected

characteristic: particulate organic carbon (mg/L)

characteristic: pcr conditions

text: Open-Bio bioplastics

text: plastisphere metagenome

text: software

text: Illumina MiSeq

text: 16S/18S SSU rRNA

text: 400bp, 600bp

characteristic: title

text: not collected


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Royal NIOZ

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: ammonium (mg/L)

text: Mediterranean Sea Biome [ENVO:01000047]

characteristic: broad-scale environmental context

characteristic: chlorophyll (µg/L)

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: dissolved organic carbon (mg/L)

characteristic: dissolved oxygen (mg/L)

characteristic: environmental medium

text: Field incubation, Time series

text: Mediterranean Sea

text: Coastal zone [ENVO:00000486]

text: FWD (Golay57): CGTTTGGAATGA; REV (BC52): GCGGTCGGTCAG

text: Powersoil DNA Isolation kit

text: plastisphere metagenome

text: not collected

characteristic: pcr primers

characteristic: phaeopigments (µg/L)

characteristic: phosphate (mg/L)

characteristic: polymer disintegration (%)

text: Open-Bio bioplastics

characteristic: project name

characteristic: salinity

text: plastisphere metagenome

text: software

characteristic: sequence quality check

text: Illumina MiSeq

characteristic: sequencing method

characteristic: silicate (mg/L)

text: 16S/18S SSU rRNA

characteristic: target gene

text: 400bp, 600bp

characteristic: targeted amplicon size

characteristic: temperature

characteristic: total depth of water column

text: not collected

characteristic: total inorganic nitrogen (mg/L)

characteristic: total nitrogen (mg/L)

characteristic: total phosphorus (mg/L)