E-MTAB-13083:Sample 1

status: PUBLIC

characteristic: ENA first public

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: External Id

text: UOM

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13083:Sample 1

characteristic: Submitter Id

text: 2 to 3

unit: month

characteristic: age

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: house mouse

characteristic: common name

text: Protocols: For circadian time series whole lumbar IVDs from 2-3-month-old male and female WT and Col2a1-Bmal1-/- mice were collected 4 hours apart over a period of 48 hours, starting at 9 am. Two biological replicates were obtained per genotype and timepoint. Whole lumbar IVDs were resected, and paraspinal tissues carefully removed. Tissues were immediately snap frozen and stored at -80oC for later processing. For AF tissue RNAseq male and female three-month-old WT and Col2a1-Bmal1-/- mice were collected at 8 am. Three biological replicates were obtained per genotype and timepoint. Tail discs were resected, and the NP and AF were manually separated under a dissection microscope to ensure careful separation of tissue. AF tissues were kept on ice and kept hydrated in HBSS throughout the dissection procedure, then snap frozen and stored at -80oC for later processing. All animal studies were performed in accordance with the 1986 UK Home Office Animal Procedures Act. Approval was provided by the local ethics committee. Mice were maintained at 20-22°C, with standard rodent chow available ad libitum and under 12:12 hr light dark schedule (light on at 7 am; light off at 7 pm). The PER2::Luc mice carry the firefly luciferase gene fused in-frame with the 3’ end of the Per2 gene, creating a fusion protein reporter (Yoo et al. PNAS 2004). Bmal1flox/flox - PER2::Luc mice were subsequently crossed with Col2a1-Cre mice expressing Cre recombinase under the control of the Col2a1 promoter3 to generate IVD /cartilage specific Bmal1 KO. All mice were bred in-house at the University of Manchester. Generation and genotyping of the Col2a1-Bmal1-/- mice was described before (Dudek et al. JCI 2016) For the isolation of RNA tissues were homogenised in TRIzol using a Mikro-Dismembrator S (Satorius Stedim Biotech) at 2000 rpm for 30 seconds, twice. Liquid nitrogen was used to keep tissues and homogenates cold at all times during the homogenisation process. Homogenates were subsequently processed using 1-Bromo-3-chloropropane to separate RNA. The aqueous phase was processed twice to ensure clean separation of RNA from other organic matter. Glycogen was used to capture mRNA. Pellets were washed three times in 70% EtOH, dried and resuspended in RNAse-free H2O. The quantity of resuspended mRNA was measured using a QubitTM 4 Fluorometer (Thermo Fisher Scientific) and RNA integrity measured using a 2200 TapeStation (Agilent Technologies) to ensure good quality for downstream analysis. Libraries were generated by the Genomic Technologies Core Facility using the TruSeq® Stranded mRNA assay (Illumina, Inc.) according to the manufacturer’s protocol

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Bmal1 KO

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

characteristic: isolate

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lumbar intervertebral disc

characteristic: organism part

text: Mus musculus

text: C57BL/6J PER2::Luc

characteristic: strain

text: Sample 1

characteristic: title


E-MTAB-13083:Sample 10

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: UOM

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13083:Sample 10

text: 2 to 3

text: ArrayExpress

text: not collected

text: house mouse

text: adult

text: normal

text: wild type genotype

text: not collected

text: not applicable

text: Mus musculus

text: Mus musculus

text: annulus fibrosus

text: Mus musculus

text: C57BL/6J PER2::Luc

text: Sample 10


E-MTAB-13083:Sample 11

text: UOM

text: public

text: E-MTAB-13083:Sample 11

characteristic: Submitter Id

text: 2 to 3

characteristic: age

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

text: Bmal1 KO

text: not collected

text: not applicable

text: Mus musculus

text: Mus musculus

text: annulus fibrosus

text: Mus musculus

text: C57BL/6J PER2::Luc

text: Sample 11


E-MTAB-13083:Sample 12

text: UOM

text: public

text: E-MTAB-13083:Sample 12

text: 2 to 3

text: ArrayExpress

text: not collected

text: house mouse

text: adult

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

characteristic: isolate

text: Mus musculus

characteristic: organism

text: Mus musculus

text: annulus fibrosus

characteristic: organism part

text: Mus musculus

text: C57BL/6J PER2::Luc

text: Sample 12


E-MTAB-13083:Sample 13

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: UOM

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-13083:Sample 13

text: 2 to 3

text: ArrayExpress

text: not collected

text: house mouse

text: adult

text: normal

text: Bmal1 KO

text: not collected

text: not applicable

text: Mus musculus

text: Mus musculus

text: annulus fibrosus

text: Mus musculus

text: C57BL/6J PER2::Luc

characteristic: strain

text: Sample 13

characteristic: title


E-MTAB-13083:Sample 14

text: UOM

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13083:Sample 14

characteristic: Submitter Id

text: 2 to 3

characteristic: age

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: house mouse

characteristic: common name

text: adult

text: normal

text: wild type genotype

text: not collected

text: not applicable

text: Mus musculus

text: Mus musculus

text: annulus fibrosus

text: Mus musculus

text: C57BL/6J PER2::Luc

text: Sample 14


E-MTAB-13083:Sample 15

text: UOM

text: public

text: E-MTAB-13083:Sample 15

text: 2 to 3

text: ArrayExpress

text: not collected

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Bmal1 KO

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

characteristic: individual

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: lumbar intervertebral disc

text: Mus musculus

text: C57BL/6J PER2::Luc

text: Sample 15


E-MTAB-13083:Sample 16

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: UOM

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-13083:Sample 16

text: 2 to 3

text: ArrayExpress

text: not collected

text: house mouse

text: adult

text: normal

text: Bmal1 KO

text: not collected

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lumbar intervertebral disc

characteristic: organism part

text: Mus musculus

text: C57BL/6J PER2::Luc

text: Sample 16


E-MTAB-13083:Sample 17

text: UOM

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13083:Sample 17

characteristic: Submitter Id

text: 2 to 3

characteristic: age

text: ArrayExpress

characteristic: broker name

text: not collected

text: house mouse

text: adult

text: normal

text: Bmal1 KO

text: not collected

text: not applicable

text: Mus musculus

text: Mus musculus

text: lumbar intervertebral disc

text: Mus musculus

text: C57BL/6J PER2::Luc

characteristic: strain

text: Sample 17

characteristic: title


E-MTAB-13083:Sample 18

text: UOM

text: public

text: E-MTAB-13083:Sample 18

text: 2 to 3

text: ArrayExpress

text: not collected

characteristic: collection date

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Bmal1 KO

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

text: Mus musculus

text: Mus musculus

text: lumbar intervertebral disc

text: Mus musculus

text: C57BL/6J PER2::Luc

text: Sample 18


E-MTAB-13083:Sample 19

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: UOM

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-13083:Sample 19

text: 2 to 3

text: ArrayExpress

text: not collected

text: house mouse

text: adult

text: normal

text: Bmal1 KO

text: not collected

characteristic: individual

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: lumbar intervertebral disc

text: Mus musculus

text: C57BL/6J PER2::Luc

text: Sample 19


E-MTAB-13083:Sample 2

text: UOM

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13083:Sample 2

characteristic: Submitter Id

text: 2 to 3

characteristic: age

text: ArrayExpress

characteristic: broker name

text: not collected

text: house mouse

text: adult

text: normal

text: Bmal1 KO

text: not collected

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lumbar intervertebral disc

characteristic: organism part

text: Mus musculus

text: C57BL/6J PER2::Luc

text: Sample 2


E-MTAB-13083:Sample 20

text: UOM

text: public

text: E-MTAB-13083:Sample 20

text: 2 to 3

text: ArrayExpress

text: not collected

characteristic: collection date

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Bmal1 KO

text: not collected

text: not applicable

text: Mus musculus

text: Mus musculus

text: lumbar intervertebral disc

text: Mus musculus

text: C57BL/6J PER2::Luc

characteristic: strain

text: Sample 20

characteristic: title


E-MTAB-13083:Sample 21

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: UOM

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-13083:Sample 21

text: 2 to 3

text: ArrayExpress

text: not collected

text: house mouse

text: adult

text: normal

text: Bmal1 KO

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

text: Mus musculus

text: Mus musculus

text: lumbar intervertebral disc

text: Mus musculus

text: C57BL/6J PER2::Luc

text: Sample 21


E-MTAB-13083:Sample 22

text: UOM

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13083:Sample 22

characteristic: Submitter Id

text: 2 to 3

characteristic: age

text: ArrayExpress

characteristic: broker name

text: not collected

text: house mouse

text: adult

text: normal

text: Bmal1 KO

text: not collected

characteristic: individual

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: lumbar intervertebral disc

text: Mus musculus

text: C57BL/6J PER2::Luc

text: Sample 22


E-MTAB-13083:Sample 23

text: UOM

text: public

text: E-MTAB-13083:Sample 23

text: 2 to 3

text: ArrayExpress

text: not collected

characteristic: collection date

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Bmal1 KO

text: not collected

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lumbar intervertebral disc

characteristic: organism part

text: Mus musculus

text: C57BL/6J PER2::Luc

text: Sample 23


E-MTAB-13083:Sample 24

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: UOM

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-13083:Sample 24

text: 2 to 3

text: ArrayExpress

text: not collected

text: house mouse

text: adult

text: normal

text: wild type genotype

text: not collected

text: not applicable

text: Mus musculus

text: Mus musculus

text: lumbar intervertebral disc

text: Mus musculus

text: C57BL/6J PER2::Luc

characteristic: strain

text: Sample 24

characteristic: title


E-MTAB-13083:Sample 25

text: UOM

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13083:Sample 25

characteristic: Submitter Id

text: 2 to 3

characteristic: age

text: ArrayExpress

characteristic: broker name

text: not collected

text: house mouse

text: adult

text: normal

text: wild type genotype

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

text: Mus musculus

text: Mus musculus

text: lumbar intervertebral disc

text: Mus musculus

text: C57BL/6J PER2::Luc

text: Sample 25


E-MTAB-13083:Sample 26

text: UOM

text: public

text: E-MTAB-13083:Sample 26

text: 2 to 3

text: ArrayExpress

text: not collected

characteristic: collection date

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

text: not collected

characteristic: individual

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: lumbar intervertebral disc

text: Mus musculus

text: C57BL/6J PER2::Luc

text: Sample 26


E-MTAB-13083:Sample 27

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: UOM

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-13083:Sample 27

text: 2 to 3

text: ArrayExpress

text: not collected

text: house mouse

text: adult

text: normal

text: wild type genotype

text: not collected

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lumbar intervertebral disc

characteristic: organism part

text: Mus musculus

text: C57BL/6J PER2::Luc

text: Sample 27


E-MTAB-13083:Sample 28

text: UOM

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13083:Sample 28

characteristic: Submitter Id

text: 2 to 3

characteristic: age

text: ArrayExpress

characteristic: broker name

text: not collected

text: house mouse

text: adult

text: normal

text: wild type genotype

text: not collected

text: not applicable

text: Mus musculus

text: Mus musculus

text: lumbar intervertebral disc

text: Mus musculus

text: C57BL/6J PER2::Luc

characteristic: strain

text: Sample 28

characteristic: title


E-MTAB-13083:Sample 29

text: UOM

text: public

text: E-MTAB-13083:Sample 29

text: 2 to 3

text: ArrayExpress

text: not collected

characteristic: collection date

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

text: Mus musculus

text: Mus musculus

text: lumbar intervertebral disc

text: Mus musculus

text: C57BL/6J PER2::Luc

text: Sample 29


E-MTAB-13083:Sample 3

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: UOM

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-13083:Sample 3

text: 2 to 3

text: ArrayExpress

text: not collected

text: house mouse

text: adult

text: normal

text: Bmal1 KO

text: not collected

characteristic: individual

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: lumbar intervertebral disc

text: Mus musculus

text: C57BL/6J PER2::Luc

text: Sample 3


E-MTAB-13083:Sample 30

text: UOM

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13083:Sample 30

characteristic: Submitter Id

text: 2 to 3

characteristic: age

text: ArrayExpress

characteristic: broker name

text: not collected

text: house mouse

text: adult

text: normal

text: wild type genotype

text: not collected

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lumbar intervertebral disc

characteristic: organism part

text: Mus musculus

text: C57BL/6J PER2::Luc

text: Sample 30


E-MTAB-13083:Sample 4

text: UOM

text: public

text: E-MTAB-13083:Sample 4

text: 2 to 3

text: ArrayExpress

text: not collected

characteristic: collection date

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

text: not collected

text: not applicable

text: Mus musculus

text: Mus musculus

text: lumbar intervertebral disc

text: Mus musculus

text: C57BL/6J PER2::Luc

characteristic: strain

text: Sample 4

characteristic: title


E-MTAB-13083:Sample 5

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: UOM

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-13083:Sample 5

text: 2 to 3

text: ArrayExpress

text: not collected

text: house mouse

text: adult

text: normal

text: wild type genotype

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

text: Mus musculus

text: Mus musculus

text: lumbar intervertebral disc

text: Mus musculus

text: C57BL/6J PER2::Luc

text: Sample 5


E-MTAB-13083:Sample 6

text: UOM

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13083:Sample 6

characteristic: Submitter Id

text: 2 to 3

characteristic: age

text: ArrayExpress

characteristic: broker name

text: not collected

text: house mouse

text: adult

text: normal

text: wild type genotype

text: not collected

characteristic: individual

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: lumbar intervertebral disc

text: Mus musculus

text: C57BL/6J PER2::Luc

text: Sample 6


E-MTAB-13083:Sample 7

text: UOM

text: public

text: E-MTAB-13083:Sample 7

text: 2 to 3

text: ArrayExpress

text: not collected

characteristic: collection date

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

text: not collected

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lumbar intervertebral disc

characteristic: organism part

text: Mus musculus

text: C57BL/6J PER2::Luc

text: Sample 7


E-MTAB-13083:Sample 8

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: UOM

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-13083:Sample 8

text: 2 to 3

text: ArrayExpress

text: not collected

text: house mouse

text: adult

text: normal

text: wild type genotype

text: not collected

text: not applicable

text: Mus musculus

text: Mus musculus

text: lumbar intervertebral disc

text: Mus musculus

text: C57BL/6J PER2::Luc

characteristic: strain

text: Sample 8

characteristic: title


E-MTAB-13083:Sample 9

text: UOM

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13083:Sample 9

characteristic: Submitter Id

text: 2 to 3

characteristic: age

text: ArrayExpress

characteristic: broker name

text: not collected

text: house mouse

text: adult

text: normal

text: Bmal1 KO

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

text: Mus musculus

text: Mus musculus

text: annulus fibrosus

text: Mus musculus

text: C57BL/6J PER2::Luc

text: Sample 9


text: Luxembourg Institute of Health

text: public

tag: Namespace:Luxembourg Institute of Health

characteristic: collection date

text: house mouse

characteristic: common name

text: Fecal sample at day 17 from mouse (ID 131) colonised with 14SM and switched to a FF diet (noCr)

characteristic: description

text: USA

text: Mus musculus

text: Mus musculus


characteristic: ENA first public

text: University of Exeter

text: public

tag: Namespace:University of Exeter

text: Ireland

text: Eunicella verrucosa

characteristic: organism

text: Eunicella verrucosa


characteristic: ENA-CHECKLIST

text: School of Cancer Sciences, University of Glasgow

text: public

text: 12 to 25

unit: week

text: ArrayExpress

text: mature gamma-delta T cell

text: not collected

text: house mouse

text: Protocols: Cells were FACS sorted (DAPI-CD3+TCRD+CD27+, then Vg1+ or Vg4+ followed by gating on Ly6C positive or negative) from pooled lymph nodes (inguinal, brachial and axillary) and spleens of 10 wildytpe female C57BL/6 mice per biological replicate raw sequence quality was assessed using FastQC v. 0.11.8, trimmed using FastP, trimmed sequences aligned to mouse genome using STAR, raw counts per gene determined using FeatureCounts v.1.6.4 RNA isolation was performed using Qiagen RNeasy Mini Kit (74106) according to manufacturer's instructions SMARTer Stranded Total RNA-seq v2 - Pico Input Mammalian (Takara)

text: adult

characteristic: developmental stage

text: wild type genotype

text: not collected

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: spleen and lymph nodes

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex


characteristic: INSDC center name

text: public

characteristic: INSDC status

text: 12 to 25

text: ArrayExpress

text: gamma-delta T cell

text: not collected

text: house mouse

text: adult

text: wild type genotype

text: not collected

text: not applicable

text: Mus musculus

text: Mus musculus

text: spleen and lymph nodes

text: Mus musculus

text: female

characteristic: strain

characteristic: title


unit: BP

characteristic: Age

text: SGN BiK-F

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: broad-scale environmental context

unit: m

characteristic: depth

text: Sediment samples from EMB262;tag=EMB262_6_28_MUC_14

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

characteristic: local environmental context

text: tctactga:tgacatca

characteristic: multiplex identifiers

text: no

characteristic: negative control type

text: uncultured eukaryote

text: Forward=TTTGTCTGSTTAATTSCG;Reverse=CACAGACCTGTTATTGC

characteristic: pcr primers

text: no

unit: e

characteristic: positive control type

text: Dia/Dino metabarcoding for comparison

characteristic: project name

unit: g

characteristic: sample volume or weight for DNA extraction

text: uncultured eukaryote

text: MiSeq V3

characteristic: sequencing method

text: 18S rRNA gene

characteristic: target gene

characteristic: target subfragment


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

characteristic: description

text: soil

text: Baltic Sea

characteristic: geographic location (country and/or sea)

text: brackish water

text: gcgtcagc:tgacatca

text: no

text: uncultured eukaryote

characteristic: organism

text: no

text: Dia/Dino metabarcoding for comparison

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene

characteristic: title


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

characteristic: local environmental context

text: tactatac:tgacatca

characteristic: multiplex identifiers

text: no

characteristic: negative control type

text: uncultured eukaryote

characteristic: pcr primers

text: no

characteristic: positive control type

text: Dia/Dino metabarcoding for comparison

characteristic: project name

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

text: tcgcgctg:tgacatca

text: no

text: uncultured eukaryote

text: no

text: Dia/Dino metabarcoding for comparison

characteristic: sample volume or weight for DNA extraction

text: uncultured eukaryote

text: MiSeq V3

characteristic: sequencing method

text: 18S rRNA gene

characteristic: target gene

characteristic: target subfragment


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

text: Sediment samples from EMB262;tag=EMB262_6_28_MUC_1

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

characteristic: local environmental context

text: ctatgcta:cgtataca

characteristic: multiplex identifiers

text: no

text: uncultured eukaryote

characteristic: organism

text: no

text: Dia/Dino metabarcoding for comparison

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene

characteristic: title


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

text: atcagtca:cgtataca

text: no

characteristic: negative control type

text: uncultured eukaryote

characteristic: pcr primers

text: no

characteristic: positive control type

text: Dia/Dino metabarcoding for comparison

characteristic: project name

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

text: agactatg:cgtataca

text: no

text: uncultured eukaryote

text: no

text: Dia/Dino metabarcoding for comparison

characteristic: sample volume or weight for DNA extraction

text: uncultured eukaryote

text: MiSeq V3

characteristic: sequencing method

text: 18S rRNA gene

characteristic: target gene

characteristic: target subfragment


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

characteristic: local environmental context

text: tagtcgca:cgtataca

characteristic: multiplex identifiers

text: no

text: uncultured eukaryote

characteristic: organism

text: no

text: Dia/Dino metabarcoding for comparison

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene

characteristic: title


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

text: Sediment samples from EMB262;tag=EMB262_6_28_MUC_5

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

text: acgacgag:tcgcgctg

text: no

characteristic: negative control type

text: uncultured eukaryote

characteristic: pcr primers

text: no

characteristic: positive control type

text: Dia/Dino metabarcoding for comparison

characteristic: project name

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

text: gtcacgtc:tcgcgctg

text: no

text: uncultured eukaryote

text: no

text: Dia/Dino metabarcoding for comparison

characteristic: sample volume or weight for DNA extraction

text: uncultured eukaryote

text: MiSeq V3

characteristic: sequencing method

text: 18S rRNA gene

characteristic: target gene

characteristic: target subfragment


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

characteristic: local environmental context

text: gtgtacat:tcgcgctg

characteristic: multiplex identifiers

text: no

text: uncultured eukaryote

characteristic: organism

text: no

text: Dia/Dino metabarcoding for comparison

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene

characteristic: title


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

text: actctgct:tcgcgctg

text: no

characteristic: negative control type

text: uncultured eukaryote

characteristic: pcr primers

text: no

characteristic: positive control type

text: Dia/Dino metabarcoding for comparison

characteristic: project name

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

text: Sediment samples from EMB262;tag=EMB262_6_28_MUC_12

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

text: agcgacta:tctactga

text: no

text: uncultured eukaryote

text: no

text: Dia/Dino metabarcoding for comparison

characteristic: sample volume or weight for DNA extraction

text: uncultured eukaryote

text: MiSeq V3

characteristic: sequencing method

text: 18S rRNA gene

characteristic: target gene

characteristic: target subfragment


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

characteristic: local environmental context

text: acacacac:tctactga

characteristic: multiplex identifiers

text: no

text: uncultured eukaryote

characteristic: organism

text: no

text: Dia/Dino metabarcoding for comparison

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene

characteristic: title


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

text: gtacgact:tctactga

text: no

characteristic: negative control type

text: uncultured eukaryote

characteristic: pcr primers

text: no

characteristic: positive control type

text: Dia/Dino metabarcoding for comparison

characteristic: project name

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

text: cgctctcg:tctactga

text: no

text: uncultured eukaryote

text: no

text: Dia/Dino metabarcoding for comparison

characteristic: sample volume or weight for DNA extraction

text: uncultured eukaryote

text: MiSeq V3

characteristic: sequencing method

text: 18S rRNA gene

characteristic: target gene

characteristic: target subfragment


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

text: Sediment samples from EMB262;tag=EMB262_13_8_MUC_21

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

characteristic: local environmental context

text: catcagtc:cacatgat

characteristic: multiplex identifiers

text: no

text: uncultured eukaryote

characteristic: organism

text: no

text: Dia/Dino metabarcoding for comparison

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene

characteristic: title


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

text: atatagcg:cacatgat

text: no

characteristic: negative control type

text: uncultured eukaryote

characteristic: pcr primers

text: no

characteristic: positive control type

text: Dia/Dino metabarcoding for comparison

characteristic: project name

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

text: gactgatg:cacatgat

text: no

text: uncultured eukaryote

text: no

text: Dia/Dino metabarcoding for comparison

characteristic: sample volume or weight for DNA extraction

text: uncultured eukaryote

text: MiSeq V3

characteristic: sequencing method

text: 18S rRNA gene

characteristic: target gene

characteristic: target subfragment


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

characteristic: local environmental context

text: tatgtcag:cacatgat

characteristic: multiplex identifiers

text: no

text: uncultured eukaryote

characteristic: organism

text: no

text: Dia/Dino metabarcoding for comparison

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene

characteristic: title


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

text: Sediment samples from EMB262;tag=EMB262_13_8_MUC_22

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

text: agactatg:cacatgat

text: no

characteristic: negative control type

text: uncultured eukaryote

characteristic: pcr primers

text: no

characteristic: positive control type

text: Dia/Dino metabarcoding for comparison

characteristic: project name

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

text: atcagtca:cacatgat

text: no

text: uncultured eukaryote

text: no

text: Dia/Dino metabarcoding for comparison

characteristic: sample volume or weight for DNA extraction

text: uncultured eukaryote

text: MiSeq V3

characteristic: sequencing method

text: 18S rRNA gene

characteristic: target gene

characteristic: target subfragment


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

characteristic: local environmental context

text: tagtcgca:cacatgat

characteristic: multiplex identifiers

text: no

text: uncultured eukaryote

characteristic: organism

text: no

text: Dia/Dino metabarcoding for comparison

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene

characteristic: title


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

text: ctatgcta:cacatgat

text: no

characteristic: negative control type

text: uncultured eukaryote

characteristic: pcr primers

text: no

characteristic: positive control type

text: Dia/Dino metabarcoding for comparison

characteristic: project name

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

text: Sediment samples from EMB262;tag=EMB262_13_8_MUC_26

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

text: tgacatca:agactatg

text: no

text: uncultured eukaryote

text: no

text: Dia/Dino metabarcoding for comparison

characteristic: sample volume or weight for DNA extraction

text: uncultured eukaryote

text: MiSeq V3

characteristic: sequencing method

text: 18S rRNA gene

characteristic: target gene

characteristic: target subfragment


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

characteristic: local environmental context

text: gatgatct:agactatg

characteristic: multiplex identifiers

text: no

text: uncultured eukaryote

characteristic: organism

text: no

text: Dia/Dino metabarcoding for comparison

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene

characteristic: title


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

text: agcacagt:agactatg

text: no

characteristic: negative control type

text: uncultured eukaryote

characteristic: pcr primers

text: no

characteristic: positive control type

text: Dia/Dino metabarcoding for comparison

characteristic: project name

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

text: actagatc:agactatg

text: no

text: uncultured eukaryote

text: no

text: Dia/Dino metabarcoding for comparison

characteristic: sample volume or weight for DNA extraction

text: uncultured eukaryote

text: MiSeq V3

characteristic: sequencing method

text: 18S rRNA gene

characteristic: target gene

characteristic: target subfragment


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

text: Sediment samples from EMB262;tag=EMB262_13_8_MUC_23

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

characteristic: local environmental context

text: atcagtca:tactatac

characteristic: multiplex identifiers

text: no

text: uncultured eukaryote

characteristic: organism

text: no

text: Dia/Dino metabarcoding for comparison

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene

characteristic: title


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

text: agactatg:tactatac

text: no

characteristic: negative control type

text: uncultured eukaryote

characteristic: pcr primers

text: no

characteristic: positive control type

text: Dia/Dino metabarcoding for comparison

characteristic: project name

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

text: tagtcgca:tactatac

text: no

text: uncultured eukaryote

text: no

text: Dia/Dino metabarcoding for comparison

characteristic: sample volume or weight for DNA extraction

text: uncultured eukaryote

text: MiSeq V3

characteristic: sequencing method

text: 18S rRNA gene

characteristic: target gene

characteristic: target subfragment


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

characteristic: local environmental context

text: ctatgcta:tactatac

characteristic: multiplex identifiers

text: no

text: uncultured eukaryote

characteristic: organism

text: no

text: Dia/Dino metabarcoding for comparison

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene

characteristic: title


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

text: Sediment samples from EMB262;tag=EMB262_13_8_MUC_25

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

text: agcgacta:acatgtgt

text: no

characteristic: negative control type

text: uncultured eukaryote

characteristic: pcr primers

text: no

characteristic: positive control type

text: Dia/Dino metabarcoding for comparison

characteristic: project name

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

text: acacacac:acatgtgt

text: no

text: uncultured eukaryote

text: no

text: Dia/Dino metabarcoding for comparison

characteristic: sample volume or weight for DNA extraction

text: uncultured eukaryote

text: MiSeq V3

characteristic: sequencing method

text: 18S rRNA gene

characteristic: target gene

characteristic: target subfragment


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

characteristic: local environmental context

text: gtacgact:acatgtgt

characteristic: multiplex identifiers

text: no

text: uncultured eukaryote

characteristic: organism

text: no

text: Dia/Dino metabarcoding for comparison

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene

characteristic: title


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

text: cgctctcg:acatgtgt

text: no

characteristic: negative control type

text: uncultured eukaryote

characteristic: pcr primers

text: no

characteristic: positive control type

text: Dia/Dino metabarcoding for comparison

characteristic: project name

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

text: Sediment samples from EMB262;tag=EMB262_6_28_MUC_16

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

text: atatagcg:ctatgcta

text: no

text: uncultured eukaryote

text: no

text: Dia/Dino metabarcoding for comparison

characteristic: sample volume or weight for DNA extraction

text: uncultured eukaryote

text: MiSeq V3

characteristic: sequencing method

text: 18S rRNA gene

characteristic: target gene

characteristic: target subfragment


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

characteristic: local environmental context

text: gactgatg:ctatgcta

characteristic: multiplex identifiers

text: no

text: uncultured eukaryote

characteristic: organism

text: no

text: Dia/Dino metabarcoding for comparison

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene

characteristic: title


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

text: catcagtc:ctatgcta

text: no

characteristic: negative control type

text: uncultured eukaryote

characteristic: pcr primers

text: no

characteristic: positive control type

text: Dia/Dino metabarcoding for comparison

characteristic: project name

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

text: tatgtcag:ctatgcta

text: no

text: uncultured eukaryote

text: no

text: Dia/Dino metabarcoding for comparison

characteristic: sample volume or weight for DNA extraction

text: uncultured eukaryote

text: MiSeq V3

characteristic: sequencing method

text: 18S rRNA gene

characteristic: target gene

characteristic: target subfragment


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

text: Sediment samples from EMB262;tag=EMB262_6_28_MUC_7

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

characteristic: local environmental context

text: gtcacgtc:gtacgact

characteristic: multiplex identifiers

text: no

text: uncultured eukaryote

characteristic: organism

text: no

text: Dia/Dino metabarcoding for comparison

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene

characteristic: title


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

text: gtgtacat:gtacgact

text: no

characteristic: negative control type

text: uncultured eukaryote

characteristic: pcr primers

text: no

characteristic: positive control type

text: Dia/Dino metabarcoding for comparison

characteristic: project name

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

text: actctgct:gtacgact

text: no

text: uncultured eukaryote

text: no

text: Dia/Dino metabarcoding for comparison

characteristic: sample volume or weight for DNA extraction

text: uncultured eukaryote

text: MiSeq V3

characteristic: sequencing method

text: 18S rRNA gene

characteristic: target gene

characteristic: target subfragment


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

characteristic: local environmental context

text: acgacgag:gtacgact

characteristic: multiplex identifiers

text: no

text: uncultured eukaryote

characteristic: organism

text: no

text: Dia/Dino metabarcoding for comparison

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene

characteristic: title


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

text: Sediment samples from EMB262;tag=EMB262_6_28_MUC_13

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

text: atcagtca:ctatgcta

text: no

characteristic: negative control type

text: uncultured eukaryote

characteristic: pcr primers

text: no

characteristic: positive control type

text: Dia/Dino metabarcoding for comparison

characteristic: project name

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

text: ctatgcta:ctatgcta

text: no

text: uncultured eukaryote

text: no

text: Dia/Dino metabarcoding for comparison

characteristic: sample volume or weight for DNA extraction

text: uncultured eukaryote

text: MiSeq V3

characteristic: sequencing method

text: 18S rRNA gene

characteristic: target gene

characteristic: target subfragment


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

characteristic: local environmental context

text: tagtcgca:ctatgcta

characteristic: multiplex identifiers

text: no

text: uncultured eukaryote

characteristic: organism

text: no

text: Dia/Dino metabarcoding for comparison

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene

characteristic: title


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

text: agactatg:ctatgcta

text: no

characteristic: negative control type

text: uncultured eukaryote

characteristic: pcr primers

text: no

characteristic: positive control type

text: Dia/Dino metabarcoding for comparison

characteristic: project name

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

text: Sediment samples from EMB262;tag=EMB262_13_8_MUC_19

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

text: gcgtcagc:ctatgcta

text: no

text: uncultured eukaryote

text: no

text: Dia/Dino metabarcoding for comparison

characteristic: sample volume or weight for DNA extraction

text: uncultured eukaryote

text: MiSeq V3

characteristic: sequencing method

text: 18S rRNA gene

characteristic: target gene

characteristic: target subfragment


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

characteristic: local environmental context

text: tcgcgctg:ctatgcta

characteristic: multiplex identifiers

text: no

text: uncultured eukaryote

characteristic: organism

text: no

text: Dia/Dino metabarcoding for comparison

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene

characteristic: title


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

text: tctactga:ctatgcta

text: no

characteristic: negative control type

text: uncultured eukaryote

characteristic: pcr primers

text: no

characteristic: positive control type

text: Dia/Dino metabarcoding for comparison

characteristic: project name

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

text: tactatac:ctatgcta

text: no

text: uncultured eukaryote

text: no

text: Dia/Dino metabarcoding for comparison

characteristic: sample volume or weight for DNA extraction

text: uncultured eukaryote

text: MiSeq V3

characteristic: sequencing method

text: 18S rRNA gene

characteristic: target gene

characteristic: target subfragment


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

text: Sediment samples from EMB262;tag=EMB262_13_8_MUC_18

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

characteristic: local environmental context

text: tactatac:atgatcgc

characteristic: multiplex identifiers

text: no

text: uncultured eukaryote

characteristic: organism

text: no

text: Dia/Dino metabarcoding for comparison

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene

characteristic: title


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

text: tcgcgctg:atgatcgc

text: no

characteristic: negative control type

text: uncultured eukaryote

characteristic: pcr primers

text: no

characteristic: positive control type

text: Dia/Dino metabarcoding for comparison

characteristic: project name

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

text: gcgtcagc:atgatcgc

text: no

text: uncultured eukaryote

text: no

text: Dia/Dino metabarcoding for comparison

characteristic: sample volume or weight for DNA extraction

text: uncultured eukaryote

text: MiSeq V3

characteristic: sequencing method

text: 18S rRNA gene

characteristic: target gene

characteristic: target subfragment


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

characteristic: local environmental context

text: tctactga:atgatcgc

characteristic: multiplex identifiers

text: no

text: uncultured eukaryote

characteristic: organism

text: no

text: Dia/Dino metabarcoding for comparison

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene

characteristic: title


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

text: Sediment samples from EMB262;tag=EMB262_6_28_MUC_18

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

text: tactatac:agtgctac

text: no

characteristic: negative control type

text: uncultured eukaryote

characteristic: pcr primers

text: no

characteristic: positive control type

text: Dia/Dino metabarcoding for comparison

characteristic: project name

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

text: tcgcgctg:agtgctac

text: no

text: uncultured eukaryote

text: no

text: Dia/Dino metabarcoding for comparison

characteristic: sample volume or weight for DNA extraction

text: uncultured eukaryote

text: MiSeq V3

characteristic: sequencing method

text: 18S rRNA gene

characteristic: target gene

characteristic: target subfragment


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

characteristic: local environmental context

text: tctactga:agtgctac

characteristic: multiplex identifiers

text: no

text: uncultured eukaryote

characteristic: organism

text: no

text: Dia/Dino metabarcoding for comparison

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene

characteristic: title


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

text: gcgtcagc:agtgctac

text: no

characteristic: negative control type

text: uncultured eukaryote

characteristic: pcr primers

text: no

characteristic: positive control type

text: Dia/Dino metabarcoding for comparison

characteristic: project name

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

text: Sediment samples from EMB262;tag=EMB262_13_8_MUC_17

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

text: atatagcg:gtgtacat

text: no

text: uncultured eukaryote

text: no

text: Dia/Dino metabarcoding for comparison

characteristic: sample volume or weight for DNA extraction

text: uncultured eukaryote

text: MiSeq V3

characteristic: sequencing method

text: 18S rRNA gene

characteristic: target gene

characteristic: target subfragment


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

characteristic: local environmental context

text: catcagtc:gtgtacat

characteristic: multiplex identifiers

text: no

text: uncultured eukaryote

characteristic: organism

text: no

text: Dia/Dino metabarcoding for comparison

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene

characteristic: title


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

text: gactgatg:gtgtacat

text: no

characteristic: negative control type

text: uncultured eukaryote

characteristic: pcr primers

text: no

characteristic: positive control type

text: Dia/Dino metabarcoding for comparison

characteristic: project name

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

text: tatgtcag:gtgtacat

text: no

text: uncultured eukaryote

text: no

text: Dia/Dino metabarcoding for comparison

characteristic: sample volume or weight for DNA extraction

text: uncultured eukaryote

text: MiSeq V3

characteristic: sequencing method

text: 18S rRNA gene

characteristic: target gene

characteristic: target subfragment


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

text: Sediment samples from EMB262;tag=EMB262_6_28_MUC_3

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

characteristic: local environmental context

text: tgacatca:gtcacgtc

characteristic: multiplex identifiers

text: no

text: uncultured eukaryote

characteristic: organism

text: no

text: Dia/Dino metabarcoding for comparison

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene

characteristic: title


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

text: actagatc:gtcacgtc

text: no

characteristic: negative control type

text: uncultured eukaryote

text: Forward=TTTGTCTGSTTAATTSCG;Reverse=CACAGACCTGTTATTGC

characteristic: pcr primers

text: no

characteristic: positive control type

text: Dia/Dino metabarcoding for comparison

characteristic: project name

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

text: agcacagt:gtcacgtc

text: no

text: uncultured eukaryote

text: no

text: Dia/Dino metabarcoding for comparison

characteristic: sample volume or weight for DNA extraction

text: uncultured eukaryote

text: MiSeq V3

characteristic: sequencing method

text: 18S rRNA gene

characteristic: target gene

characteristic: target subfragment


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

characteristic: local environmental context

text: gatgatct:gtcacgtc

characteristic: multiplex identifiers

text: no

text: uncultured eukaryote

characteristic: organism

text: no

text: Dia/Dino metabarcoding for comparison

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene

characteristic: title


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

text: Sediment samples from EMB262;tag=EMB262_6_28_MUC_20

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

text: tcgcgctg:tagtcgca

text: no

characteristic: negative control type

text: uncultured eukaryote

characteristic: pcr primers

text: no

characteristic: positive control type

text: Dia/Dino metabarcoding for comparison

characteristic: project name

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

text: tctactga:tagtcgca

text: no

text: uncultured eukaryote

text: no

text: Dia/Dino metabarcoding for comparison

characteristic: sample volume or weight for DNA extraction

text: uncultured eukaryote

text: MiSeq V3

characteristic: sequencing method

text: 18S rRNA gene

characteristic: target gene

characteristic: target subfragment


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

characteristic: local environmental context

text: tactatac:tagtcgca

characteristic: multiplex identifiers

text: no

text: uncultured eukaryote

characteristic: organism

text: no

text: Dia/Dino metabarcoding for comparison

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene

characteristic: title


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

text: gcgtcagc:tagtcgca

text: no

characteristic: negative control type

text: uncultured eukaryote

characteristic: pcr primers

text: no

characteristic: positive control type

text: Dia/Dino metabarcoding for comparison

characteristic: project name

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

text: Sediment samples from EMB262;tag=EMB262_6_28_MUC_21

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

text: tcagtgtc:atatagcg

text: no

text: uncultured eukaryote

text: no

text: Dia/Dino metabarcoding for comparison

characteristic: sample volume or weight for DNA extraction

text: uncultured eukaryote

text: MiSeq V3

characteristic: sequencing method

text: 18S rRNA gene

characteristic: target gene

characteristic: target subfragment


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

characteristic: local environmental context

text: acagcaca:atatagcg

characteristic: multiplex identifiers

text: no

text: uncultured eukaryote

characteristic: organism

text: no

text: Dia/Dino metabarcoding for comparison

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene

characteristic: title


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

text: gtcgtaga:atatagcg

text: no

characteristic: negative control type

text: uncultured eukaryote

characteristic: pcr primers

text: no

characteristic: positive control type

text: Dia/Dino metabarcoding for comparison

characteristic: project name

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

text: atgatcgc:atatagcg

text: no

text: uncultured eukaryote

text: no

text: Dia/Dino metabarcoding for comparison

characteristic: sample volume or weight for DNA extraction

text: uncultured eukaryote

text: MiSeq V3

characteristic: sequencing method

text: 18S rRNA gene

characteristic: target gene

characteristic: target subfragment


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

text: Sediment samples from EMB262;tag=EMB262_13_8_MUC_14

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

characteristic: local environmental context

text: tactatac:acagcaca

characteristic: multiplex identifiers

text: no

text: uncultured eukaryote

characteristic: organism

text: no

text: Dia/Dino metabarcoding for comparison

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene

characteristic: title


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

text: tctactga:acagcaca

text: no

characteristic: negative control type

text: uncultured eukaryote

characteristic: pcr primers

text: no

characteristic: positive control type

text: Dia/Dino metabarcoding for comparison

characteristic: project name

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

text: gcgtcagc:acagcaca

text: no

text: uncultured eukaryote

text: no

text: Dia/Dino metabarcoding for comparison

characteristic: sample volume or weight for DNA extraction

text: uncultured eukaryote

text: MiSeq V3

characteristic: sequencing method

text: 18S rRNA gene

characteristic: target gene

characteristic: target subfragment


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

characteristic: local environmental context

text: tcgcgctg:acagcaca

characteristic: multiplex identifiers

text: no

text: uncultured eukaryote

characteristic: organism

text: no

text: Dia/Dino metabarcoding for comparison

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene

characteristic: title


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

text: Sediment samples from EMB262;tag=EMB262_6_28_MUC_17

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

text: tgacatca:cgctctcg

text: no

characteristic: negative control type

text: uncultured eukaryote

characteristic: pcr primers

text: no

characteristic: positive control type

text: Dia/Dino metabarcoding for comparison

characteristic: project name

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

text: gatgatct:cgctctcg

text: no

text: uncultured eukaryote

text: no

text: Dia/Dino metabarcoding for comparison

characteristic: sample volume or weight for DNA extraction

text: uncultured eukaryote

text: MiSeq V3

characteristic: sequencing method

text: 18S rRNA gene

characteristic: target gene

characteristic: target subfragment


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

characteristic: local environmental context

text: actagatc:cgctctcg

characteristic: multiplex identifiers

text: no

text: uncultured eukaryote

characteristic: organism

text: no

text: Dia/Dino metabarcoding for comparison

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene

characteristic: title


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

text: agcacagt:cgctctcg

text: no

characteristic: negative control type

text: uncultured eukaryote

characteristic: pcr primers

text: no

characteristic: positive control type

text: Dia/Dino metabarcoding for comparison

characteristic: project name

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

text: Sediment samples from EMB262;tag=EMB262_6_28_MUC_23

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

text: acgacgag:cacatgat

text: no

text: uncultured eukaryote

text: no

text: Dia/Dino metabarcoding for comparison

characteristic: sample volume or weight for DNA extraction

text: uncultured eukaryote

text: MiSeq V3

characteristic: sequencing method

text: 18S rRNA gene

characteristic: target gene

characteristic: target subfragment


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

characteristic: local environmental context

text: gtcacgtc:cacatgat

characteristic: multiplex identifiers

text: no

text: uncultured eukaryote

characteristic: organism

text: no

text: Dia/Dino metabarcoding for comparison

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene

characteristic: title


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

text: actctgct:cacatgat

text: no

characteristic: negative control type

text: uncultured eukaryote

characteristic: pcr primers

text: no

characteristic: positive control type

text: Dia/Dino metabarcoding for comparison

characteristic: project name

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

text: gtgtacat:cacatgat

text: no

text: uncultured eukaryote

text: no

text: Dia/Dino metabarcoding for comparison

characteristic: sample volume or weight for DNA extraction

text: uncultured eukaryote

text: MiSeq V3

characteristic: sequencing method

text: 18S rRNA gene

characteristic: target gene

characteristic: target subfragment


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

text: Sediment samples from EMB262;tag=EMB262_6_28_MUC_22

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

characteristic: local environmental context

text: tactatac:tatgtcag

characteristic: multiplex identifiers

text: no

text: uncultured eukaryote

characteristic: organism

text: no

text: Dia/Dino metabarcoding for comparison

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene

characteristic: title


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

text: gcgtcagc:tatgtcag

text: no

characteristic: negative control type

text: uncultured eukaryote

characteristic: pcr primers

text: no

characteristic: positive control type

text: Dia/Dino metabarcoding for comparison

characteristic: project name

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

text: tctactga:tatgtcag

text: no

text: uncultured eukaryote

text: no

text: Dia/Dino metabarcoding for comparison

characteristic: sample volume or weight for DNA extraction

text: uncultured eukaryote

text: MiSeq V3

characteristic: sequencing method

text: 18S rRNA gene

characteristic: target gene

characteristic: target subfragment


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

characteristic: local environmental context

text: tcgcgctg:tatgtcag

characteristic: multiplex identifiers

text: no

text: uncultured eukaryote

characteristic: organism

text: no

text: Dia/Dino metabarcoding for comparison

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene

characteristic: title


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

text: Sediment samples from EMB262;tag=EMB262_13_8_MUC_9

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

text: catcagtc:cgtataca

text: no

characteristic: negative control type

text: uncultured eukaryote

characteristic: pcr primers

text: no

characteristic: positive control type

text: Dia/Dino metabarcoding for comparison

characteristic: project name

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

text: atatagcg:cgtataca

text: no

text: uncultured eukaryote

text: no

text: Dia/Dino metabarcoding for comparison

characteristic: sample volume or weight for DNA extraction

text: uncultured eukaryote

text: MiSeq V3

characteristic: sequencing method

text: 18S rRNA gene

characteristic: target gene

characteristic: target subfragment


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

characteristic: local environmental context

text: gactgatg:cgtataca

characteristic: multiplex identifiers

text: no

text: uncultured eukaryote

characteristic: organism

text: no

text: Dia/Dino metabarcoding for comparison

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene

characteristic: title


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

text: tatgtcag:cgtataca

text: no

characteristic: negative control type

text: uncultured eukaryote

characteristic: pcr primers

text: no

characteristic: positive control type

text: Dia/Dino metabarcoding for comparison

characteristic: project name

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

text: Sediment samples from EMB262;tag=EMB262_6_28_MUC_34

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

text: atcagtca:ctgcgtac

text: no

text: uncultured eukaryote

text: no

text: Dia/Dino metabarcoding for comparison

characteristic: sample volume or weight for DNA extraction

text: uncultured eukaryote

text: MiSeq V3

characteristic: sequencing method

text: 18S rRNA gene

characteristic: target gene

characteristic: target subfragment


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

characteristic: local environmental context

text: agactatg:ctgcgtac

characteristic: multiplex identifiers

text: no

text: uncultured eukaryote

characteristic: organism

text: no

text: Dia/Dino metabarcoding for comparison

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene

characteristic: title


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

text: ctatgcta:ctgcgtac

text: no

characteristic: negative control type

text: uncultured eukaryote

characteristic: pcr primers

text: no

characteristic: positive control type

text: Dia/Dino metabarcoding for comparison

characteristic: project name

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

text: tagtcgca:ctgcgtac

text: no

text: uncultured eukaryote

text: no

text: Dia/Dino metabarcoding for comparison

characteristic: sample volume or weight for DNA extraction

text: uncultured eukaryote

text: MiSeq V3

characteristic: sequencing method

text: 18S rRNA gene

characteristic: target gene

characteristic: target subfragment


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

text: Sediment samples from EMB262;tag=EMB262_6_28_MUC_11

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

characteristic: local environmental context

text: tatgtcag:acgacgag

characteristic: multiplex identifiers

text: no

text: uncultured eukaryote

characteristic: organism

text: no

text: Dia/Dino metabarcoding for comparison

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene

characteristic: title


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

text: gactgatg:acgacgag

text: no

characteristic: negative control type

text: uncultured eukaryote

characteristic: pcr primers

text: no

characteristic: positive control type

text: Dia/Dino metabarcoding for comparison

characteristic: project name

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene


text: SGN BiK-F

text: public

text: aquatic biome

characteristic: broad-scale environmental context

characteristic: depth

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: brackish water

text: atatagcg:acgacgag

text: no

text: uncultured eukaryote

text: no

text: Dia/Dino metabarcoding for comparison

characteristic: sample volume or weight for DNA extraction

text: uncultured eukaryote

text: MiSeq V3

characteristic: sequencing method

text: 18S rRNA gene

characteristic: target gene

characteristic: target subfragment


characteristic: Age

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: SGN BiK-F

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: aquatic biome

characteristic: collection date

text: soil

text: Baltic Sea

text: brackish water

characteristic: local environmental context

text: catcagtc:acgacgag

characteristic: multiplex identifiers

text: no

text: uncultured eukaryote

characteristic: organism

text: no

text: Dia/Dino metabarcoding for comparison

text: uncultured eukaryote

text: MiSeq V3

text: 18S rRNA gene

characteristic: title