status: PUBLIC

characteristic: ENA first public

tag: attribute

characteristic: ENA-CHECKLIST

text: Southern University of Science and Technology

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

unit: day

characteristic: age

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: house mouse

characteristic: common name

text: Protocols: Dissection of VZs from neonatal mouse brains was performed as described 1. Briefly, the midbrain, the olfactory bulb, meninges and the hippocampus were removed in sequence to attain the telencephalon. Then VZ-containing tissues were separated from the telencephalon. Tissues from 4 mice were pooled, transferred to a 1.5ml Eppendorf tube, and digested with freshly prepared 10U/ml Papain solution (Worthington, catalog no. LS003126) at 37°C for 30 minutes. Then the Papain solution was aspirated, and 1ml culture medium (20% FBS) was added, followed by 1 minute of incubation to terminate the digestion. The supernatant was removed, and another 1ml culture medium (20% FBS) was added. The digested tissues were gently pipetted up and down by 20 times with a P1000 tip to dissociate the cells mechanically. Centrifuge at 350rcf for 5 minutes and discard the supernatant.  DNA was purified first by Qiagen minElute PCR purification kit according to the manufacturer's instruction, then by AmupureXP beads with 0.5X upper cutoff and a 1.2X lower cutoff according to manufacturer's instruction. Libraries were constructed in the Illumina Nextera DNA Library preparation kit.

characteristic: description

text: EPC

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

characteristic: isolate

text: Mus musculus

characteristic: organism

text: Mus musculus

text: ventricular zone

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex

characteristic: strain

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: ArrayExpress

text: not collected

text: house mouse

text: EPC

text: wild type genotype

text: not collected

text: not applicable

text: Mus musculus

text: Mus musculus

text: ventricular zone

text: Mus musculus

text: siNC

characteristic: stimulus


text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: house mouse

text: EPC

text: wild type genotype

text: not collected

text: not applicable

text: Mus musculus

text: Mus musculus

text: ventricular zone

text: Mus musculus

text: siTfeb


text: public

text: ArrayExpress

text: P0 CD133 glial cell

text: not collected

text: house mouse

characteristic: common name

text: Protocols: Dissection of VZs from neonatal mouse brains was performed as described 1. Briefly, the midbrain, the olfactory bulb, meninges and the hippocampus were removed in sequence to attain the telencephalon. Then VZ-containing tissues were separated from the telencephalon. Tissues from 4 mice were pooled, transferred to a 1.5ml Eppendorf tube, and digested with freshly prepared 10U/ml Papain solution (Worthington, catalog no. LS003126) at 37°C for 30 minutes. Then the Papain solution was aspirated, and 1ml culture medium (20% FBS) was added, followed by 1 minute of incubation to terminate the digestion. The supernatant was removed, and another 1ml culture medium (20% FBS) was added. The digested tissues were gently pipetted up and down by 20 times with a P1000 tip to dissociate the cells mechanically. Centrifuge at 350rcf for 5 minutes and discard the supernatant. DNA was purified first by Qiagen minElute PCR purification kit according to the manufacturer's instruction, then by AmupureXP beads with 0.5X upper cutoff and a 1.2X lower cutoff according to manufacturer's instruction. Libraries were constructed using the 10x Genomics Gene Expression 3' kit according to the manual.

characteristic: description

text: radial glia

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

text: Mus musculus

text: Mus musculus

text: ventricular zone

text: Mus musculus

text: 10x Genomics Gene Expression 3'

characteristic: single-cell platform


text: public

text: ArrayExpress

text: P0 CD133 glial cell

text: not collected

text: house mouse

text: radial glia

text: wild type genotype

text: not collected

text: not applicable

characteristic: isolate

text: Mus musculus

characteristic: organism

text: Mus musculus

text: ventricular zone

characteristic: organism part

text: Mus musculus

text: mcSCRB-seq

characteristic: strain

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: ArrayExpress

text: P5 CD133 glial cell

text: not collected

text: house mouse

text: radial glia

text: wild type genotype

text: not collected

text: not applicable

text: Mus musculus

text: Mus musculus

text: ventricular zone

text: Mus musculus

text: 10x Genomics Gene Expression 3'


text: Max Planck Institute for Evolutionary Anthropology

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: human

text: Prehispanic human remains

text: Mexico

text: Chich�n Itz�, Yucatan

characteristic: geographic location (region and locality)

text: 20� 40' N, 88� 34' W

text: Homo sapiens

text: Homo sapiens

text: Male

characteristic: sex

text: Left petrous bone


text: ITPA

text: public

text: S1_KB2P_CRISPR_WholeGenome_Cisplatin_3720_rep1

characteristic: collection date

text: house mouse

characteristic: common name

text: sgRNA plasmid reads from LoF screen in KB2P cell line

characteristic: description

text: Switzerland

text: Mus musculus

text: Mus musculus


text: ITPA

text: public

text: S2_KB2P_CRISPR_WholeGenome_Cisplatin_3720_rep2

text: house mouse

text: Switzerland

characteristic: geographic location (country and/or sea)

text: Mus musculus

text: Mus musculus


text: ITPA

text: public

text: S3_KB2P_CRISPR_WholeGenome_Cisplatin_3720_rep3

text: house mouse

text: Switzerland

text: Mus musculus

text: Mus musculus


text: ITPA

text: public

text: S4_KB2P_CRISPR_WholeGenome_Cisplatin_3720_rep1

text: house mouse

text: Switzerland

text: Mus musculus

text: Mus musculus


text: ITPA

text: public

text: S5_KB2P_CRISPR_WholeGenome_Cisplatin_3720_rep2

text: house mouse

text: Switzerland

text: Mus musculus

text: Mus musculus


text: ITPA

text: public

text: S6_KB2P_CRISPR_WholeGenome_Cisplatin_3720_rep3

text: house mouse

text: Switzerland

text: Mus musculus

text: Mus musculus


text: ITPA

text: public

text: S7_KB2P_CRISPR_WholeGenome_Cisplatin_3720_rep1

text: house mouse

text: Switzerland

text: Mus musculus

text: Mus musculus

text: S7_KB2P121_cisplatin025_rep1_GATCTG_3720


text: ITPA

text: public

text: S8_KB2P_CRISPR_WholeGenome_Cisplatin_3720_rep2

text: house mouse

text: Switzerland

text: Mus musculus

text: Mus musculus

text: S8_KB2P121_cisplatin025_rep2_TCAAGT_3564


text: ITPA

text: public

text: S9_KB2P_CRISPR_WholeGenome_Cisplatin_3720_rep3

text: house mouse

text: Switzerland

text: Mus musculus

text: Mus musculus

text: S9_KB2P121_cisplatin025_rep3_CTGATC_3564


text: NIUST

text: public

text: house mouse

text: Mice feces

text: China

text: Mus musculus

text: Mus musculus


characteristic: External Id

text: University of Cologne

characteristic: INSDC last update

text: public

text: ena-SAMPLE-TAB-21-04-2022-07:53:50:693-55941

tag: Namespace:University of Cologne

text: zebrafish

text: Targeted PacBio sequencing of selected immune genes of 93 zebrafish individuals

text: Danio rerio

text: Danio rerio

text: PacBio Zebrafish Immunity