status: PUBLIC

characteristic: ENA first public

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Forest Botany and Tree Physiology, University of Geottingen

characteristic: Submitter Id

unit: week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: Populus x canescens

characteristic: cultivar

text: Protocols: Per plant: harvest of full leaf (3,4,5) without petiole, pooling and freezing in liquid nitrogen Populus × canescens plants were in vitro propagated in glass jars (weck jars) according to Müller et al. 2013 Frontiers in Plant Science. Four week old Populus × canescens were co-cultivated with Laccaria bicolor (sandwich culture) according to Müller et al. 2013 Frontiers in Plant Science. The co-cultures were incubated for one, two, four and seven days until sample collection. The experiment was independently repeated in three times. Total RNA was extracted with innuPrep Plant RNA kit (Analytik Jena, Jena, Germany). The RNA of three samples was pooled. The library was constructed according to the manufacturer's protocol

characteristic: description

text: LP.07 seven leaves visible stage

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

characteristic: growth condition

characteristic: infect

text: not applicable

characteristic: isolate

text: Populus tremula

characteristic: organism

text: Populus tremula

text: leaf

characteristic: organism part

characteristic: replicate

text: mixed leaf three four and five

characteristic: sampling site

text: Populus tremula

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: Forest Botany and Tree Physiology, University of Geottingen

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: Populus x canescens

text: LP.07 seven leaves visible stage

text: wild type genotype

text: not applicable

text: Populus tremula

text: Populus tremula

text: leaf

text: mixed leaf three four and five

text: Populus tremula


text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: Populus x canescens

characteristic: cultivar

characteristic: description

text: LP.07 seven leaves visible stage

text: wild type genotype

text: control harvested directly from an assembled sandwich system

text: not applicable

text: Populus tremula

text: Populus tremula

text: leaf

text: mixed leaf three four and five

text: Populus tremula


text: public

text: ArrayExpress

text: Populus x canescens

text: LP.07 seven leaves visible stage

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: in vitro propagation in glass jar (weck jar)

characteristic: growth condition

characteristic: infect

text: not applicable

characteristic: isolate

text: Populus tremula

characteristic: organism

text: Populus tremula

text: leaf

text: mixed leaf three four and five

text: Populus tremula


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: public

text: ArrayExpress

text: Populus x canescens

text: LP.07 seven leaves visible stage

text: wild type genotype

text: Laccaria bicolor treatment on roots (co-cultivation, sandwich system)

text: Laccaria bicolor

text: not applicable

text: Populus tremula

text: Populus tremula

text: leaf

characteristic: organism part

characteristic: replicate

text: mixed leaf three four and five

characteristic: sampling site

text: Populus tremula

characteristic: title


characteristic: External Id

text: UNIVERSITY OF AMSTERDAM

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:UNIVERSITY OF AMSTERDAM

characteristic: Submitter Id

unit: g

characteristic: amount or size of sample collected

text: Botrytis cinerea (B05.10)

characteristic: biotic regimen

text: methanol (aerial) for 24 h before inoculation

characteristic: chemical administration

text: Krešimir Šola

characteristic: collection date

text: thale cress

characteristic: common name

text: Netherlands

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: North Holland, Amsterdam

characteristic: geographic location (region and locality)

text: growth chamber

characteristic: growth facility

characteristic: isolation and growth condition

unit: h

characteristic: light duration per day (24h)

characteristic: light regimen

text: Arabidopsis thaliana

text: Arabidopsis

characteristic: organism common name

characteristic: plant developmental stage

text: soil

characteristic: plant growth medium

characteristic: plant structure

text: diploid

characteristic: ploidy

text: infected (Botrytis cinerea)

characteristic: sample disease status

text: healthy

characteristic: sample health state

text: 5 weeks

characteristic: sampled age

text: Arabidopsis thaliana


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: UNIVERSITY OF AMSTERDAM

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: Botrytis cinerea (B05.10)

characteristic: biotic regimen

text: (Z)-3-hexenol in methanol (aerial) for 24 h before inoculation

characteristic: chemical administration

text: Krešimir Šola

characteristic: collection date

text: thale cress

characteristic: common name

characteristic: genotype

text: Netherlands

text: North Holland, Amsterdam

text: growth chamber

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis

text: soil

text: diploid

text: infected (Botrytis cinerea)

text: healthy

text: 5 weeks

text: Arabidopsis thaliana

characteristic: title


text: UNIVERSITY OF AMSTERDAM

text: public

text: Botrytis cinerea (B05.10)

text: (E)-2-hexenal in methanol (aerial) for 24 h before inoculation

text: Krešimir Šola

text: thale cress

text: Netherlands

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: North Holland, Amsterdam

characteristic: geographic location (region and locality)

text: growth chamber

characteristic: growth facility

characteristic: isolation and growth condition

characteristic: light duration per day (24h)

characteristic: light regimen

text: Arabidopsis thaliana

text: Arabidopsis

characteristic: organism common name

characteristic: plant developmental stage

text: soil

characteristic: plant growth medium

characteristic: plant structure

text: diploid

characteristic: ploidy

text: infected (Botrytis cinerea)

characteristic: sample disease status

text: healthy

characteristic: sample health state

text: 5 weeks

characteristic: sampled age

text: Arabidopsis thaliana


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: UNIVERSITY OF AMSTERDAM

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: Botrytis cinerea (B05.10)

characteristic: biotic regimen

text: (Z)-3-hexenyl acetate in methanol (aerial) for 24 h before inoculation

characteristic: chemical administration

text: Krešimir Šola

characteristic: collection date

text: thale cress

characteristic: common name

characteristic: genotype

text: Netherlands

text: North Holland, Amsterdam

text: growth chamber

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis

text: soil

text: diploid

text: infected (Botrytis cinerea)

text: healthy

text: 5 weeks

text: Arabidopsis thaliana

characteristic: title


text: UNIVERSITY OF AMSTERDAM

text: public

text: Botrytis cinerea (B05.10)

text: Krešimir Šola

text: thale cress

text: Netherlands

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: North Holland, Amsterdam

characteristic: geographic location (region and locality)

text: growth chamber

characteristic: growth facility

characteristic: isolation and growth condition

characteristic: light duration per day (24h)

characteristic: light regimen

text: Arabidopsis thaliana

text: Arabidopsis

characteristic: organism common name

characteristic: plant developmental stage

text: soil

characteristic: plant growth medium

characteristic: plant structure

text: diploid

characteristic: ploidy

text: infected (Botrytis cinerea)

characteristic: sample disease status

text: diseased

characteristic: sample health state

text: 5 weeks

characteristic: sampled age

text: Arabidopsis thaliana


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: UNIVERSITY OF AMSTERDAM

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: Botrytis cinerea (B05.10)

characteristic: biotic regimen

characteristic: chemical administration

text: Krešimir Šola

characteristic: collection date

text: thale cress

characteristic: common name

characteristic: genotype

text: Netherlands

text: North Holland, Amsterdam

text: growth chamber

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis

text: soil

text: diploid

text: infected (Botrytis cinerea)

text: diseased

text: 5 weeks

text: Arabidopsis thaliana

characteristic: title


text: UNIVERSITY OF AMSTERDAM

text: public

text: Botrytis cinerea (B05.10)

text: Krešimir Šola

text: thale cress

text: Netherlands

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: North Holland, Amsterdam

characteristic: geographic location (region and locality)

text: growth chamber

characteristic: growth facility

characteristic: isolation and growth condition

characteristic: light duration per day (24h)

characteristic: light regimen

text: Arabidopsis thaliana

text: Arabidopsis

characteristic: organism common name

characteristic: plant developmental stage

text: soil

characteristic: plant growth medium

characteristic: plant structure

text: diploid

characteristic: ploidy

text: infected (Botrytis cinerea)

characteristic: sample disease status

text: diseased

characteristic: sample health state

text: 5 weeks

characteristic: sampled age

text: Arabidopsis thaliana


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: UNIVERSITY OF AMSTERDAM

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: Botrytis cinerea (B05.10)

characteristic: biotic regimen

characteristic: chemical administration

text: Krešimir Šola

characteristic: collection date

text: thale cress

characteristic: common name

characteristic: genotype

text: Netherlands

text: North Holland, Amsterdam

text: growth chamber

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis

text: soil

text: diploid

text: infected (Botrytis cinerea)

text: diseased

text: 5 weeks

text: Arabidopsis thaliana

characteristic: title


text: mixed

characteristic: ArrayExpress-SEX

text: Panagrolaimus detritophagus

characteristic: ArrayExpress-SPECIES

text: Mixed

characteristic: GENDER

text: SC

text: public

text: Panagrolaimus detritophagus

text: Panagrolaimus detritophagus

text: Panagrolaimus detritophagus


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: webin-liuxiaokun

text: public

characteristic: description

text: China

characteristic: geographic location (country and/or sea)

text: metagenome

text: metagenome


text: Swansea University

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Swansea University

characteristic: Submitter Id

text: Brazil

text: Kryptolebias hermaphroditus

text: Kryptolebias hermaphroditus


text: Swansea University

text: public

characteristic: collection date

text: Brazil

text: Kryptolebias ocellatus

text: Kryptolebias ocellatus


text: Royal Botanic Gardens, Kew

text: public

tag: Namespace:Royal Botanic Gardens, Kew

text: 99 7055 /0

text: de Vos, J.M.

text: not provided

text: s.n.

characteristic: collectorNo

text: not provided

text: ZSS:Living_collection:99 7055 /0

text: Disocactus phyllanthoides

text: Disocactus phyllanthoides

text: de Vos, J.M. s.n.; 99 7055 /0 (ZSS); 99 7055 /0

text: Disocactus phyllanthoides


text: Royal Botanic Gardens, Kew

text: public

text: Luteyn, J.L.; Pedraza L.P.

text: not provided

text: Gonocalyx megabracteolatus

characteristic: organism

text: Gonocalyx megabracteolatus

text: Luteyn, J.L.; Pedraza L.P. 15566 (K); K000993527

text: Gonocalyx megabracteolatus


text: Royal Botanic Gardens, Kew

text: public

text: not provided

text: Ecclinusa ramiflora

text: Ecclinusa ramiflora

text: Amorim, A.; Sant'Ana, S.; Santos, T.S.; Brito, H.S.; dos Santos, E.B. 465 (K); K001400204

text: Ecclinusa ramiflora

characteristic: title


text: Royal Botanic Gardens, Kew

text: public

text: Silva, L.A.M.

text: not provided

text: Holoregmia viscida

text: Holoregmia viscida

text: Silva, L.A.M. 2297 (K); K001395040

text: Holoregmia viscida


text: public

tag: Namespace:MAX PLANCK INSTITUTE FOR MARINE MICROBIOLOGY

text: marine biome [ENVO:00000447]

characteristic: broad-scale environmental context

unit: m

characteristic: depth

text: A 500 ml surface seawater sample was sequentially filtered through a 10, 3 and 0.2 µm pore-size polycarbonate membrane filter. A DNA extraction was subsequently performed on the 0.2 – 3 µm fraction using Qiagen PowerSoil Pro Kit.

text: sea water [ENVO: 00002149]

characteristic: environmental medium

text: Canada

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Yukon; Herschel Island

characteristic: geographic location (region and locality)

text: neritic pelagic zone biome [ENVO: 01000032]

characteristic: local environmental context

text: marine metagenome

characteristic: project name

unit: °C

characteristic: sample storage temperature

text: marine metagenome

text: HER19_SLC_1000 surface seawater sample


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: MAX PLANCK INSTITUTE FOR MARINE MICROBIOLOGY

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: marine biome [ENVO:00000447]

characteristic: description

text: sea water [ENVO: 00002149]

text: Canada

characteristic: geographic location (country and/or sea)

text: Yukon; Herschel Island

text: marine metagenome

text: marine metagenome

text: HER19_SLD_250 surface seawater sample


text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: marine biome [ENVO:00000447]

characteristic: collection date

text: sea water [ENVO: 00002149]

text: Canada

text: Yukon; Herschel Island

text: marine metagenome

characteristic: organism

text: marine metagenome

text: HER19_SLT_250 surface seawater sample

characteristic: title


text: public

text: marine biome [ENVO:00000447]

characteristic: broad-scale environmental context

characteristic: depth

text: sea water [ENVO: 00002149]

characteristic: environmental medium

text: Canada

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Yukon; Herschel Island

characteristic: geographic location (region and locality)

text: marine metagenome

text: marine metagenome

text: HER19_SIP_2000 surface seawater sample


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

text: marine biome [ENVO:00000447]

text: A 200 ml surface seawater sample was sequentially filtered through a 10, 3 and 0.2 µm pore-size polycarbonate membrane filter. Nucleic acids were subsequently extracted from the 0.2 – 3 µm fraction using ZymoBIOMICS DNA/RNA Miniprep Kit.

text: sea water [ENVO: 00002149]

text: Canada

text: Yukon; Herschel Island

characteristic: local environmental context

text: marine metagenome

characteristic: project name

characteristic: sample storage temperature

text: marine metagenome

text: HER22_SLD_50 surface seawater sample


characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: marine biome [ENVO:00000447]

characteristic: description

text: sea water [ENVO: 00002149]

text: Canada

characteristic: geographic location (country and/or sea)

text: Yukon; Herschel Island

text: marine metagenome

text: marine metagenome

text: HER22_SLD_2000 surface seawater sample


text: public

characteristic: Submitter Id

text: marine biome [ENVO:00000447]

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

text: sea water [ENVO: 00002149]

text: Canada

text: Yukon; Herschel Island

text: marine metagenome

characteristic: organism

text: marine metagenome

text: HER22_SLC_100 surface seawater sample

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: public

text: marine biome [ENVO:00000447]

text: sea water [ENVO: 00002149]

characteristic: environmental medium

text: Canada

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Yukon; Herschel Island

characteristic: geographic location (region and locality)

text: marine metagenome

text: marine metagenome

text: HER22_SIP_1000 surface seawater sample


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: marine biome [ENVO:00000447]

text: sea water [ENVO: 00002149]

text: Canada

text: Yukon; Herschel Island

characteristic: local environmental context

text: marine metagenome

characteristic: project name

characteristic: sample storage temperature

text: marine metagenome

text: HER19_SLC_50 surface seawater sample


text: Stockholm University

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Stockholm University

characteristic: Submitter Id

text: terrestrial biome

characteristic: broad-scale environmental context

text: differential expression, first replicate, bud

characteristic: description

text: soil

text: Spain

characteristic: geographic location (country and/or sea)

text: temperate shrubland biome

text: Linum trigynum

text: differential expression

text: Linum trigynum


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Stockholm University

text: public

text: terrestrial biome

characteristic: collection date

text: differential expression, second replicate, bud

text: soil

text: Spain

text: temperate shrubland biome

text: Linum trigynum

characteristic: organism

text: differential expression

text: Linum trigynum

characteristic: title


characteristic: External Id

text: Stockholm University

text: public

text: terrestrial biome

text: differential expression, third replicate, bud

text: soil

characteristic: environmental medium

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: temperate shrubland biome

text: Linum trigynum

text: differential expression

text: Linum trigynum


text: Stockholm University

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: terrestrial biome

text: differential expression, first replicate, leaf

text: soil

text: Spain

text: temperate shrubland biome

characteristic: local environmental context

text: Linum trigynum

text: differential expression

characteristic: project name

text: Linum trigynum


text: Stockholm University

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: terrestrial biome

text: differential expression, second replicate, leaf

text: soil

text: Spain

text: temperate shrubland biome

text: Linum trigynum

text: differential expression

text: Linum trigynum


text: Stockholm University

text: public

text: terrestrial biome

characteristic: broad-scale environmental context

text: differential expression, third replicate, leaf

characteristic: description

text: soil

text: Spain

characteristic: geographic location (country and/or sea)

text: temperate shrubland biome

text: Linum trigynum

text: differential expression

text: Linum trigynum


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Stockholm University

text: public

text: terrestrial biome

characteristic: collection date

text: genome annotation, first replicate, bud

text: soil

text: Spain

text: temperate shrubland biome

text: Linum trigynum

text: genome annotation

text: Linum trigynum


characteristic: External Id

text: Stockholm University

text: public

text: terrestrial biome

text: genome annotation, second replicate, bud

text: soil

text: Spain

text: temperate shrubland biome

text: Linum trigynum

characteristic: organism

text: genome annotation

text: Linum trigynum

characteristic: title


text: Stockholm University

text: public

text: terrestrial biome

text: genome annotation, first replicate, leaf

text: soil

characteristic: environmental medium

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: temperate shrubland biome

text: Linum trigynum

text: genome annotation

text: Linum trigynum


text: Stockholm University

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: terrestrial biome

text: genome annotation, second replicate, leaf

text: soil

text: Spain

text: temperate shrubland biome

characteristic: local environmental context

text: Linum trigynum

text: genome annotation

text: Linum trigynum


text: Stockholm University

text: public

characteristic: INSDC status

characteristic: SRA accession

text: terrestrial biome

text: genome annotation, first replicate, stem

text: soil

text: Spain

text: temperate shrubland biome

text: Linum trigynum

text: genome annotation

characteristic: project name

text: Linum trigynum


text: Stockholm University

text: public

characteristic: Submitter Id

text: terrestrial biome

characteristic: broad-scale environmental context

text: genome annotation, second replicate, stem

characteristic: description

text: soil

text: Spain

text: temperate shrubland biome

text: Linum trigynum

text: genome annotation

text: Linum trigynum


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Stockholm University

text: public

text: terrestrial biome

text: genome annotation, first replicate, flower

text: soil

text: Spain

characteristic: geographic location (country and/or sea)

text: temperate shrubland biome

text: Linum trigynum

text: genome annotation

text: Linum trigynum


characteristic: External Id

text: Stockholm University

text: public

text: terrestrial biome

characteristic: collection date

text: genome annotation, second replicate, flower

text: soil

text: Spain

text: temperate shrubland biome

text: Linum trigynum

characteristic: organism

text: genome annotation

text: Linum trigynum


text: Stockholm University

text: public

text: terrestrial biome

text: wild sample, leaf

text: soil

characteristic: environmental medium

text: Spain

characteristic: geographic location (latitude)

text: temperate shrubland biome

text: Linum tenue

text: Linum tenue

characteristic: title


text: Stockholm University

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: terrestrial biome

text: wild sample, leaf

text: soil

text: Spain

characteristic: geographic location (longitude)

text: temperate shrubland biome

text: Linum trigynum

text: Linum trigynum


text: Stockholm University

text: public

characteristic: INSDC status

characteristic: SRA accession

text: terrestrial biome

text: Supernova genome assembly, leaf

text: soil

text: Spain

text: temperate shrubland biome

characteristic: local environmental context

text: Linum trigynum

text: genome assembly

text: Linum trigynum


text: Stockholm University

text: public

characteristic: Submitter Id

text: terrestrial biome

characteristic: broad-scale environmental context

text: Supernova genome assembly, leaf

text: soil

text: Spain

text: temperate shrubland biome

text: Linum tenue

text: genome assembly

characteristic: project name

text: Linum tenue


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Stockholm University

text: public

text: terrestrial biome

text: genome assembly, leaf

characteristic: description

text: soil

text: Spain

text: temperate shrubland biome

text: Linum trigynum

text: genome assembly

text: Linum trigynum


characteristic: External Id

text: CAMUNI

text: public

characteristic: collection date

text: Stool isolate

text: Gambia

characteristic: geographic location (country and/or sea)

text: diseased

characteristic: host health state

text: Homo sapien

characteristic: host scientific name

characteristic: isolate

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri 2a

characteristic: serovar


text: CAMUNI

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: Stool isolate

text: Gambia

text: healthy

text: Homo sapien

text: stool

text: Shigella flexneri

characteristic: organism

text: Shigella flexneri

text: S. flexneri 6

characteristic: title


text: CAMUNI

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Stool isolate

text: Gambia

text: diseased

text: Homo sapien

text: stool

text: Shigella sonnei

text: Shigella sonnei

text: S. sonnei


characteristic: ENA first public

text: CAMUNI

text: public

text: Stool isolate

text: Gambia

text: diseased

text: Homo sapien

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri 2b


characteristic: ENA-CHECKLIST

text: CAMUNI

text: public

text: Stool isolate

text: Gambia

text: diseased

text: Homo sapien

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri 1b


text: CAMUNI

text: public

text: Stool isolate

characteristic: description

text: Gambia

text: diseased

text: Homo sapien

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri 1d


characteristic: External Id

text: CAMUNI

text: public

text: Stool isolate

text: Gambia

text: diseased

text: Homo sapien

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri 3a


text: CAMUNI

text: public

characteristic: collection date

text: Stool isolate

text: Gambia

text: healthy

text: Homo sapien

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri 1b


text: CAMUNI

text: public

text: Stool isolate

text: Gambia

characteristic: geographic location (country and/or sea)

text: diseased

text: Homo sapien

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri 6


text: CAMUNI

text: public

text: Stool isolate

text: Gambia

text: healthy

characteristic: host health state

text: Homo sapien

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri 3a


text: CAMUNI

text: public

text: Stool isolate

text: Gambia

text: healthy

text: Homo sapien

characteristic: host scientific name

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri 2a


text: CAMUNI

text: public

text: Stool isolate

text: Gambia

text: healthy

text: Homo sapien

characteristic: isolate

text: stool

text: Shigella sonnei

text: Shigella sonnei

text: S. sonnei


text: CAMUNI

text: public

text: Stool isolate

text: Gambia

text: healthy

text: Homo sapien

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri 2b

characteristic: serovar


text: CAMUNI

characteristic: INSDC center name

text: public

text: Stool isolate

text: Gambia

text: diseased

text: Homo sapien

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri 1a


text: CAMUNI

characteristic: INSDC last update

text: public

text: Stool isolate

text: Gambia

text: diseased

text: Homo sapien

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri 4a


text: CAMUNI

text: public

text: Stool isolate

text: Gambia

text: healthy

text: Homo sapien

text: stool

text: Shigella flexneri

characteristic: organism

text: Shigella flexneri

text: S. flexneri 4a


text: CAMUNI

text: public

text: Stool isolate

text: Gambia

text: diseased

text: Homo sapien

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri 3b

characteristic: title


text: CAMUNI

text: public

characteristic: INSDC status

text: Stool isolate

text: Gambia

text: diseased

text: Homo sapien

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri 2a


text: CAMUNI

text: public

characteristic: SRA accession

text: Stool isolate

text: Gambia

text: healthy

text: Homo sapien

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri 6


text: CAMUNI

text: public

characteristic: Submitter Id

text: Stool isolate

text: Gambia

text: diseased

text: Homo sapien

text: stool

text: Shigella sonnei

text: Shigella sonnei

text: S. sonnei


characteristic: ENA first public

text: CAMUNI

text: public

text: Stool isolate

text: Gambia

text: diseased

text: Homo sapien

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri 7a


characteristic: ENA-CHECKLIST

text: CAMUNI

text: public

text: Stool isolate

text: Gambia

text: diseased

text: Homo sapien

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri 1b


text: CAMUNI

text: public

text: Stool isolate

characteristic: description

text: Gambia

text: diseased

text: Homo sapien

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri Y


characteristic: External Id

text: CAMUNI

text: public

text: Stool isolate

text: Gambia

text: diseased

text: Homo sapien

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri 3a


text: CAMUNI

text: public

characteristic: collection date

text: Stool isolate

text: Mali

text: diseased

text: Homo sapien

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri 2a


text: CAMUNI

text: public

text: Stool isolate

text: Mali

characteristic: geographic location (country and/or sea)

text: healthy

text: Homo sapien

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri 7a


text: CAMUNI

text: public

text: Stool isolate

text: Mali

text: diseased

characteristic: host health state

text: Homo sapien

text: stool

text: Shigella sonnei

text: Shigella sonnei

text: S. sonnei


text: CAMUNI

text: public

text: Stool isolate

text: Mali

text: healthy

text: Homo sapien

characteristic: host scientific name

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri 6


text: CAMUNI

text: public

text: Stool isolate

text: Mali

text: diseased

text: Homo sapien

characteristic: isolate

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri X


text: CAMUNI

text: public

text: Stool isolate

text: Mali

text: healthy

text: Homo sapien

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri 2a

characteristic: serovar


text: CAMUNI

characteristic: INSDC center name

text: public

text: Stool isolate

text: Mali

text: healthy

text: Homo sapien

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri 2b


text: CAMUNI

characteristic: INSDC last update

text: public

text: Stool isolate

text: Mali

text: healthy

text: Homo sapien

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri X


text: CAMUNI

text: public

text: Stool isolate

text: Kenya

text: diseased

text: Homo sapien

text: stool

text: Shigella flexneri

characteristic: organism

text: Shigella flexneri

text: S. flexneri 2a


text: CAMUNI

text: public

text: Stool isolate

text: Kenya

text: diseased

text: Homo sapien

text: stool

text: Shigella sonnei

text: Shigella sonnei

text: S. sonnei

characteristic: title


text: CAMUNI

text: public

characteristic: INSDC status

text: Stool isolate

text: Kenya

text: diseased

text: Homo sapien

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri 3a


text: CAMUNI

text: public

characteristic: SRA accession

text: Stool isolate

text: Kenya

text: diseased

text: Homo sapien

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri 1b


text: CAMUNI

text: public

characteristic: Submitter Id

text: Stool isolate

text: Kenya

text: diseased

text: Homo sapien

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri X


characteristic: ENA first public

text: CAMUNI

text: public

text: Stool isolate

text: Kenya

text: healthy

text: Homo sapien

text: stool

text: Shigella sonnei

text: Shigella sonnei

text: S. sonnei


characteristic: ENA-CHECKLIST

text: CAMUNI

text: public

text: Stool isolate

text: Kenya

text: diseased

text: Homo sapien

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri 4c


text: CAMUNI

text: public

text: Stool isolate

characteristic: description

text: Kenya

text: diseased

text: Homo sapien

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri 4b


characteristic: External Id

text: CAMUNI

text: public

text: Stool isolate

text: Kenya

text: diseased

text: Homo sapien

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri 6


text: CAMUNI

text: public

characteristic: collection date

text: Stool isolate

text: Kenya

text: healthy

text: Homo sapien

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri 2a


text: CAMUNI

text: public

text: Stool isolate

text: Kenya

characteristic: geographic location (country and/or sea)

text: healthy

text: Homo sapien

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri 4a


text: CAMUNI

text: public

text: Stool isolate

text: Kenya

text: healthy

characteristic: host health state

text: Homo sapien

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri 6


text: CAMUNI

text: public

text: Stool isolate

text: Kenya

text: healthy

text: Homo sapien

characteristic: host scientific name

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri 3a


text: CAMUNI

text: public

text: Stool isolate

text: Kenya

text: diseased

text: Homo sapien

characteristic: isolate

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri 4a


text: CAMUNI

text: public

text: Stool isolate

text: Kenya

text: diseased

text: Homo sapien

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri 7a

characteristic: serovar


text: CAMUNI

characteristic: INSDC center name

text: public

text: Stool isolate

text: Kenya

text: healthy

text: Homo sapien

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri 1b


text: CAMUNI

characteristic: INSDC last update

text: public

text: Stool isolate

text: Kenya

text: diseased

text: Homo sapien

text: stool

text: Shigella flexneri

text: Shigella flexneri

text: S. flexneri Y


text: CAMUNI

text: public

text: Stool isolate

text: Kenya

text: diseased

text: Homo sapien

text: stool

text: Shigella flexneri

characteristic: organism

text: Shigella flexneri

text: S. flexneri 2a


E-MTAB-13648:12 wk recovery 1

text: ED

text: public

text: E-MTAB-13648:12 wk recovery 1

characteristic: age

characteristic: batch

text: ArrayExpress

characteristic: broker name

text: not collected

text: house mouse

characteristic: common name

text: Protocols: Anterior pituitary cells were acutely isolated by trypsin digestion. Each cell preparation used anterior pituitary glands collected from three animals. After dissociation, cells were resuspended in 500 μL of phosphate-buffered saline supplemented with 25 mM HEPES and 5 mM EDTA (FACS buffer) and passed through a 35-μm cell strainer that was washed with a further 200 μL of the FACS buffer. Draq7 was added as a vitality marker and cells sorted using a SH800 Cell Sorter (Sony). Gates were established using wild-type pituitaries to avoid capturing eGFP negative (−ve) cells and to select single cells. Sorted single cells were resuspended into low bind Eppendorf tubes into 300 μL of Trizol and were frozen on dry ice and stored at −80 °C until sending for sequencing. RNA isolation was conducted at Azenta Life Science (South Plainfield, NJ). Total RNA was extracted using Qiagen RNeasy Plus Mini kit following manufacturer’s instructions (Qiagen, Hilden, Germany). Extracted RNA samples were quantified using Qubit Fluorometer (Life Technologies, Carlsbad, CA, USA) and RNA integrity was checked using Agilent TapeStation (Agilent Technologies, Palo Alto, CA, USA). Library preparation was conducted at Azenta Life Science (South Plainfield, NJ). Ultra-low input RNA sequencing library was prepared by using SMART-Seq v4 Ultra Low Input Kit for Sequencing was used for full-length cDNA synthesis and amplification (Clontech, Mountain View, CA), and Illumina Nextera XT (Illumina, San Diego, CA, USA) library was used for sequencing library preparation. Briefly, cDNA was fragmented, and adaptor was added using Transposase, followed by limited-cycle PCR to enrich and add index to the cDNA fragments. The sequencing library was validated on the Agilent TapeStation (Agilent Technologies, Palo Alto, CA, USA), and quantified by using Qubit Fluorometer (ThermoFisher Scientific, Waltham, MA, USA) as well as by quantitative PCR (KAPA Biosystems, Wilmington, MA, USA).

text: adult

characteristic: developmental stage

text: POMC-GFP

characteristic: genotype

text: not collected

text: 3 male mice

characteristic: individual

text: not applicable

text: Mus musculus

text: Mus musculus

text: pituitary gland

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex

text: chronic stress + 12 wk recovery

characteristic: stimulus

text: C57 BL/6

characteristic: strain

text: 12 wk recovery 1

characteristic: title


E-MTAB-13648:12 wk recovery 2

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: ED

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13648:12 wk recovery 2

characteristic: Submitter Id

text: ArrayExpress

text: not collected

characteristic: collection date

text: house mouse

characteristic: description

text: adult

text: POMC-GFP

text: not collected

characteristic: geographic location (country and/or sea)

text: 3 male mice

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: pituitary gland

text: Mus musculus

text: male

text: chronic stress + 12 wk recovery

text: C57 BL/6

text: 12 wk recovery 2


E-MTAB-13648:12 wk recovery 3

text: ED

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-13648:12 wk recovery 3

characteristic: age

characteristic: batch

text: ArrayExpress

characteristic: broker name

text: not collected

text: house mouse

characteristic: common name

text: adult

characteristic: developmental stage

text: POMC-GFP

characteristic: genotype

text: not collected

text: 3 male mice

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: pituitary gland

text: Mus musculus

text: male

text: chronic stress + 12 wk recovery

text: C57 BL/6

text: 12 wk recovery 3


E-MTAB-13648:4 wk recovery 1

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: ED

text: public

text: E-MTAB-13648:4 wk recovery 1

text: ArrayExpress

text: not collected

text: house mouse

text: adult

text: POMC-GFP

text: not collected

text: 3 male mice

characteristic: individual

text: not applicable

text: Mus musculus

text: Mus musculus

text: pituitary gland

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex

text: chronic stress + 4 wk recovery

characteristic: stimulus

text: C57 BL/6

characteristic: strain

text: 4 wk recovery 1

characteristic: title


E-MTAB-13648:4 wk recovery 2

text: ED

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13648:4 wk recovery 2

characteristic: Submitter Id

text: ArrayExpress

text: not collected

characteristic: collection date

text: house mouse

characteristic: description

text: adult

text: POMC-GFP

text: not collected

characteristic: geographic location (country and/or sea)

text: 3 male mice

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: pituitary gland

text: Mus musculus

text: male

text: chronic stress + 4 wk recovery

text: C57 BL/6

text: 4 wk recovery 2


E-MTAB-13648:4 wk recovery 3

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: ED

text: public

text: E-MTAB-13648:4 wk recovery 3

characteristic: age

characteristic: batch

text: ArrayExpress

characteristic: broker name

text: not collected

text: house mouse

characteristic: common name

text: adult

characteristic: developmental stage

text: POMC-GFP

characteristic: genotype

text: not collected

text: 3 male mice

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: pituitary gland

text: Mus musculus

text: male

text: chronic stress + 4 wk recovery

text: C57 BL/6

text: 4 wk recovery 3


E-MTAB-13648:Aged control 1

characteristic: External Id

text: ED

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-13648:Aged control 1

text: ArrayExpress

text: not collected

text: house mouse

text: adult

text: POMC-GFP

text: not collected

text: 3 male mice

characteristic: individual

text: not applicable

text: Mus musculus

text: Mus musculus

text: pituitary gland

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex

characteristic: stimulus

text: C57 BL/6

characteristic: strain

text: Aged control 1

characteristic: title


E-MTAB-13648:Aged control 2

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: ED

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13648:Aged control 2

characteristic: Submitter Id

text: ArrayExpress

text: not collected

characteristic: collection date

text: house mouse

characteristic: description

text: adult

text: POMC-GFP

text: not collected

characteristic: geographic location (country and/or sea)

text: 3 male mice

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: pituitary gland

text: Mus musculus

text: male

text: C57 BL/6

text: Aged control 2


E-MTAB-13648:Aged control 3

characteristic: External Id

text: ED

characteristic: INSDC center name

text: public

text: E-MTAB-13648:Aged control 3

characteristic: age

characteristic: batch

text: ArrayExpress

characteristic: broker name

text: not collected

text: house mouse

characteristic: common name

text: adult

characteristic: developmental stage

text: POMC-GFP

characteristic: genotype

text: not collected

text: 3 male mice

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: pituitary gland

text: Mus musculus

text: male

text: C57 BL/6

text: Aged control 3


E-MTAB-13648:Aged control 4

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: ED

characteristic: INSDC last update

text: public

text: E-MTAB-13648:Aged control 4

text: ArrayExpress

text: not collected

text: house mouse

text: adult

text: POMC-GFP

text: not collected

text: 3 male mice

characteristic: individual

text: not applicable

text: Mus musculus

text: Mus musculus

text: pituitary gland

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex

characteristic: stimulus

text: C57 BL/6

characteristic: strain

text: Aged control 4

characteristic: title


E-MTAB-13648:Chronic stress 1

characteristic: External Id

text: ED

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13648:Chronic stress 1

characteristic: Submitter Id

text: ArrayExpress

text: not collected

characteristic: collection date

text: house mouse

characteristic: description

text: adult

text: POMC-GFP

text: not collected

characteristic: geographic location (country and/or sea)

text: 3 male mice

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: pituitary gland

text: Mus musculus

text: male

text: chronic stress

text: C57 BL/6

text: Chronic stress 1


E-MTAB-13648:Chronic stress 2

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: ED

text: public

text: E-MTAB-13648:Chronic stress 2

characteristic: age

characteristic: batch

text: ArrayExpress

characteristic: broker name

text: not collected

text: house mouse

characteristic: common name

text: adult

characteristic: developmental stage

text: POMC-GFP

characteristic: genotype

text: not collected

text: 3 male mice

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: pituitary gland

text: Mus musculus

text: male

text: chronic stress

text: C57 BL/6

text: Chronic stress 2


E-MTAB-13648:Chronic stress 3

characteristic: External Id

text: ED

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-13648:Chronic stress 3

text: ArrayExpress

text: not collected

text: house mouse

text: adult

text: POMC-GFP

text: not collected

text: 3 male mice

characteristic: individual

text: not applicable

text: Mus musculus

text: Mus musculus

text: pituitary gland

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex

text: chronic stress

characteristic: stimulus

text: C57 BL/6

characteristic: strain

text: Chronic stress 3

characteristic: title


E-MTAB-13648:Control 1

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: ED

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13648:Control 1

characteristic: Submitter Id

text: ArrayExpress

text: not collected

characteristic: collection date

text: house mouse

characteristic: description

text: adult

text: POMC-GFP

text: not collected

characteristic: geographic location (country and/or sea)

text: 3 male mice

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: pituitary gland

text: Mus musculus

text: male

text: C57 BL/6

text: Control 1


E-MTAB-13648:Control 2

characteristic: External Id

text: ED

characteristic: INSDC center name

text: public

text: E-MTAB-13648:Control 2

characteristic: age

characteristic: batch

text: ArrayExpress

characteristic: broker name

text: not collected

text: house mouse

characteristic: common name

text: adult

characteristic: developmental stage

text: POMC-GFP

characteristic: genotype

text: not collected

text: 3 male mice

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: pituitary gland

text: Mus musculus

text: male

text: C57 BL/6

text: Control 2


E-MTAB-13648:Control 3

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: ED

characteristic: INSDC last update

text: public

text: E-MTAB-13648:Control 3

text: ArrayExpress

text: not collected

text: house mouse

text: adult

text: POMC-GFP

text: not collected

text: 3 male mice

characteristic: individual

text: not applicable

text: Mus musculus

text: Mus musculus

text: pituitary gland

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex

characteristic: stimulus

text: C57 BL/6

characteristic: strain

text: Control 3

characteristic: title


E-MTAB-13648:Control 4

characteristic: External Id

text: ED

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13648:Control 4

characteristic: Submitter Id

text: ArrayExpress

text: not collected

characteristic: collection date

text: house mouse

characteristic: description

text: adult

text: POMC-GFP

text: not collected

characteristic: geographic location (country and/or sea)

text: 3 male mice

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: pituitary gland

text: Mus musculus

text: male

text: C57 BL/6

text: Control 4


E-MTAB-13648:Control 5

characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: ED

text: public

text: E-MTAB-13648:Control 5

characteristic: age

characteristic: batch

text: ArrayExpress

characteristic: broker name

text: not collected

text: house mouse

characteristic: common name

text: adult

characteristic: developmental stage

text: POMC-GFP

characteristic: genotype

text: not collected

text: 3 male mice

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: pituitary gland

text: Mus musculus

text: male

text: C57 BL/6

text: Control 5


E-MTAB-13648:Control 6

characteristic: External Id

text: ED

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-13648:Control 6

text: ArrayExpress

text: not collected

text: house mouse

text: adult

text: POMC-GFP

text: not collected

text: 3 male mice

characteristic: individual

text: not applicable

text: Mus musculus

text: Mus musculus

text: pituitary gland

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex

characteristic: stimulus

text: C57 BL/6

characteristic: strain

text: Control 6

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: UKCEH

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: lowland dipterocarp rainforest

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

text: Organic layer soil sample for 16S and ITS amplicon sequencing

characteristic: description

characteristic: elevation

text: soil

characteristic: environmental medium

characteristic: experimental factor

text: Malaysia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: selective logging gap

characteristic: local environmental context

text: soil metagenome

characteristic: project name

text: soil metagenome


characteristic: ENA first public

characteristic: External Id

text: UKCEH

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: lowland dipterocarp rainforest

text: soil

text: Malaysia

text: intact closed canopy forest

text: soil metagenome

characteristic: organism

text: soil metagenome

characteristic: title


characteristic: ENA-CHECKLIST

text: University of Edinburgh

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:University of Edinburgh

characteristic: Submitter Id

text: PBMC

characteristic: collection date

text: human

characteristic: common name

text: United Kingdom

text: Homo sapiens

text: Homo sapiens


text: Section for Experimental and Translational Immunology, Department of Health Technology, Technical University of Denmark (DTU), Kongens Lyngby, Denmark

text: public

text: ArrayExpress

characteristic: broker name

text: CD8-Positive T-Lymphocytes

text: not collected

text: human

text: Protocols: Whole blood from 27 anonymous blood donors were obtained at the central blood bank at Rigshospitalet, Copenhagen, and enriched for PBMCs using density centrifugation in Leucosep tubes before cryopreservation at -150°C in FCS plus 10% DMSO PBMCs from 21 hepatitis patients were collected and cryopreserved under protocol ID #1999-P004983 “Cell Mediated Immunity in Viral hepatitis ~15,000 cells were loaded (based on 55% recovery from 27,500 sorted cells) onto a Chromium Controller to yield a maximum of 9,000 cells with an intermediate/high doublet rate (6.9%). We utilized the 10x Genomics 5’ v2 chemistry protocol supplied by 10x genomics allowing the cell barcode to be appended at the 5’-end of transcripts during capture. See https://elifesciences.org/articles/81810#s4 for further details. Downstream processing of mRNA and DNA barcodes is performed according to the manufacturer’s instructions (Chromium Next GEM Single Cell 5' Reagent Kits v2 [Dual Index], with the Feature Barcode technology for Cell Surface Protein & Immune Receptor Mapping) (10x Genomics, USA). Targeted amplification was performed for 13 cycles and the products were separated according to size into <400 bp (DNA barcode-tags) and >400 bp (the TCR sequences) using 0.6× SPRIselect beads (Beckman Coulter, B23318).

characteristic: description

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: ADT

characteristic: protocol

text: Homo sapiens


characteristic: ENA first public

text: public

text: ArrayExpress

text: CD8-Positive T-Lymphocytes

text: not collected

text: human

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: blood

text: GEX

text: Homo sapiens


characteristic: INSDC center name

text: public

text: ArrayExpress

text: CD8-Positive T-Lymphocytes

text: not collected

text: human

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: blood

text: VDJ

text: Homo sapiens


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:241-6547

text: France

text: diseased

characteristic: host health state

text: human

characteristic: host scientific name

text: blood culture

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:241-6548

text: France

text: diseased

text: human

text: blood culture

text: Staphylococcus epidermidis

characteristic: organism

text: Staphylococcus epidermidis

characteristic: title


characteristic: ENA-CHECKLIST

text: HCL

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:241-6549

text: France

text: diseased

text: human

text: blood culture

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

characteristic: SRA accession

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:241-6550

characteristic: Submitter Id

text: France

text: diseased

text: human

text: blood culture

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:241-6551

characteristic: collection date

text: France

text: diseased

text: human

text: blood culture

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:241-6552

text: France

text: diseased

text: human

text: blood culture

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:241-6553

text: France

text: diseased

text: human

text: blood culture

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:241-6554

text: France

text: diseased

text: human

text: blood culture

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:241-6555

text: France

text: diseased

text: human

text: blood culture

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:241-6556

text: France

text: diseased

text: human

text: blood culture

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:241-6557

text: France

text: diseased

text: human

text: blood culture

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:241-6558

text: France

text: diseased

text: human

text: blood culture

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:241-6559

text: France

text: diseased

text: human

text: blood culture

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:242-6560

text: France

text: diseased

text: human

text: blood culture

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:242-6561

text: France

text: diseased

text: human

text: blood culture

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:242-6562

text: France

text: diseased

text: human

text: blood culture

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:242-6563

text: France

text: diseased

text: human

text: blood culture

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:242-6564

text: France

text: diseased

text: human

text: blood culture

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:242-6565

text: France

text: diseased

text: human

text: blood culture

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:242-6566

text: France

text: diseased

text: human

text: blood culture

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:242-6567

text: France

text: diseased

text: human

text: blood culture

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:242-6568

text: France

text: diseased

text: human

text: blood culture

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:242-6569

text: France

text: diseased

text: human

text: blood culture

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:242-6570

text: France

text: diseased

text: human

text: blood culture

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:242-6571

text: France

text: diseased

text: human

text: blood culture

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:242-6572

text: France

text: diseased

text: human

text: blood culture

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:242-6573

text: France

text: diseased

text: human

text: blood culture

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:242-6574

text: France

text: diseased

text: human

text: blood culture

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:243-6594

text: France

text: diseased

text: human

text: bone and tissue sample

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:243-6595

text: France

text: diseased

text: human

text: bone and tissue sample

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:244-6596

text: France

text: diseased

text: human

text: bone and tissue sample

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:244-6597

text: France

text: diseased

text: human

text: bone and tissue sample

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:244-6598

text: France

text: diseased

text: human

text: bone and tissue sample

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:244-6599

text: France

text: diseased

text: human

text: bone and tissue sample

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:244-6600

text: France

text: diseased

text: human

text: bone and tissue sample

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:244-6609

text: France

text: diseased

text: human

text: catheter

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:244-6610

text: France

text: diseased

text: human

text: catheter

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:244-6611

text: France

text: diseased

text: human

text: catheter

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:244-6612

text: France

text: not collected

text: human

text: cutaneous sample

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:244-6613

text: France

text: not collected

text: human

text: respiratory sample

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:245-6614

text: France

text: not collected

text: human

text: unknown

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:245-6615

text: France

text: not collected

text: human

text: urine sample

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: HCL

text: public

text: ena-SAMPLE-TAB-12-02-2024-17:36:08:245-6616

text: France

text: not collected

text: human

text: urine sample

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: Royal Botanic Gardens, Kew

text: public

text: Pittier, H.

characteristic: collectorNo

text: not provided

text: unidentified

text: unidentified

text: Pittier, H. 13024 (K); K000220436

text: Cleome pilosa


text: Royal Botanic Gardens, Kew

text: public

text: Bovini M.G.

text: not provided

text: unidentified

text: unidentified

text: Bovini M.G.  3547 (RB); RB00713248

text: Cleome sp. - Bovini M.G.  3547 (RB); RB00713248


text: Royal Botanic Gardens, Kew

text: public

text: Camp, W.H.

text: not provided

text: Cleome gigantea

text: Cleome gigantea

text: E3795 (K); K000370580

text: Cleome gigantea


text: Wageningen university

text: public

characteristic: broad-scale environmental context

text: sand

characteristic: description

text: sand+biofilm

characteristic: environmental medium

text: Netherlands

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: slow_sand_filtration step

characteristic: local environmental context

text: biofilter metagenome

characteristic: project name

text: biofilter metagenome


characteristic: ENA first public

text: Wageningen university

characteristic: INSDC center name

text: public

text: effluent

text: Netherlands

characteristic: geographic location (country and/or sea)

text: slow_sand_filtration step

text: biofilter metagenome

text: biofilter metagenome


text: Wageningen university

text: public

text: influent

text: Netherlands

text: slow_sand_filtration step

text: biofilter metagenome

characteristic: organism

text: biofilter metagenome

characteristic: title


characteristic: ENA-CHECKLIST

text: UMC Utrecht

text: public

characteristic: INSDC status

text: human

characteristic: common name

text: Netherlands

text: Homo sapiens

text: Homo sapiens


text: UMC Utrecht

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Netherlands

text: Enterococcus faecium

text: Enterococcus faecium


text: Tromsoe Ecosystem Genomics

text: public

text: Chisholm GH sequence library 1 (ECOG-2-3)

characteristic: description

text: USA

text: Viridiplantae environmental sample

text: Viridiplantae environmental sample


text: Tromsoe Ecosystem Genomics

text: public

text: Chisholm GH sequence library 2 (ECOG-2-4)

text: USA

text: Viridiplantae environmental sample

text: Viridiplantae environmental sample


text: Tromsoe Ecosystem Genomics

text: public

text: Chisholm GH sequence library 3 (ECOG-2-5)

text: USA

text: Viridiplantae environmental sample

text: Viridiplantae environmental sample


text: Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Poland

text: public

text: 7 to 8

unit: month

characteristic: age

text: ArrayExpress

characteristic: broker name

text: anterior pituitary cell

text: not collected

text: pig

text: Protocols: For the experiment, the glandular parts of the pituitaries were obtained from gilts (age of 7-8 months and weight of 130-150 kg) on days 10 to 12 of the oestrous cycle, and descended for slaughter at the commercial abattoir. The day of the oestrous cycle was confirmed based on the morphology of ovaries.  The cells were washed with Dulbecco’s medium (Sigma-Aldrich, USA) enriched with 0.1% bovine serum albumin (Sigma-Aldrich, USA) and antibiotics (Antibiotic Antimycotic Solution, Merck, USA), and homogenized with scalpel blade. The homogenates were digested with 0.2% collagenase solution (Sigma-Aldrich, USA) at 37°C for 30 min. Isolated APc were centrifuged three times at (1000 × g, 10 min, room temperature) in 15 mL tubes containing Dulbecco’s medium. The remained tissue was treated with 0.2% collagenase and  0.25% pancreatin (Sigma-Aldrich, USA) for 10 min. The digestion steps were repeated until the complete digestion of the material. After each digestion, the obtained cells were collected and washed in Dulbecco’s medium, as described above. The cell suspensions were pooled and filtered through a 70 μm mesh filter. The cells were suspended in McCoy’s 5A medium (Sigma-Aldrich, USA), enriched with 10% horse serum (Sigma-Aldrich, USA) and 2.5% foetal calf serum (Sigma-Aldrich, USA) and seeded onto 6-well culture plates (1.5×10^6 cells/2 ml medium/well). The cells were preincubated for 72 h at 37°C (water-saturated atmosphere, 5% of CO2 and 95% of air). After 48 h of preincubation, 1 ml of fresh, serum-free McCoy’s 5A medium was added to each well. After preincubation media were replaced with fresh, enriched McCoy’s 5A medium and incubated for another 24 h in the presence of recombinant VIS (100 ng/mL; cat. # 8424-VF, RD System, USA).  Total RNA from APc was isolated using Extrazol (BLIRT, Poland), following the manufacturer’s protocol. The purity (A260/A280) and quantity (wavelength 260 nm, A260) of the obtained RNA samples were determined spectrophotometrically using Infinite M200 Pro (Tecan, Männedorf, Switzerland). Bioanalyzer 2100 (Agilent Technology, USA) was used to determine the RNA integrity. Only samples which passed RNA integrity number (RIN) >8 criteria were qualified for the further transcriptome high-throughput sequencing (RNA-Seq) analysis and the quantitative real-time polymerase chain reaction (qPCR) and reverse transcription polymerase chain reaction (RT-PCR) validations. Approximately 5 μg of total RNA was used to remove ribosomal RNA (rRNA) from the samples with the use of  Ribo-Zero Gold rRNA Removal Kit (Illumina, USA). The remaining RNA samples were fragmented  into short fragments using divalent cation buffers at elevated temperature (NEBNext® Magnesium RNA Fragmentation Module, NEB, USA). The obtained, samples were reverse-transcribed to cDNA using SuperScript™II Reverse Transcriptase (Invitrogen, USA). Subsequently, cDNA was used to synthesise U-labeled second-stranded DNA using E. coli DNA polymerase I (NEB, USA), RNase H (NEB, USA) and dUTPSolution (Thermo Fisher, USA). The obtained double-stranded cDNA (dscDNA) was subjected to the end repair and A-tailing processes. At the end, the obtained fragments of dscDNA were ligated with specific adaptors. Quality control analysis and the quantification of the obtained libraries were performed with the use of Agilent Technologies 2100 Bioanalyzer High Sensitivity DNA Chip.

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: not collected

characteristic: individual

text: not applicable

characteristic: isolate

text: Sus scrofa

text: Sus scrofa

text: pituitary gland

characteristic: organism part

text: Sus scrofa

text: female

characteristic: sex

characteristic: stimulus

text: Large White × Polish Landrace

characteristic: strain


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: INSDC center name

text: public

text: 7 to 8

text: ArrayExpress

text: anterior pituitary cell

text: not collected

text: pig

text: adult

text: wild type genotype

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: pituitary gland

text: Sus scrofa

text: female

text: Visfatin ng/mL

text: Large White × Polish Landrace

characteristic: title


text: IBENS

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: thale cress

characteristic: common name

text: Long-read ONT sequencing from mutant plants

characteristic: description

characteristic: ecotype

text: France

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: leaves


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

characteristic: isolate

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 1


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 2


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 3


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 4


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 5


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 6


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 7


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 8


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 9


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 10


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 11


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 12


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 13


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 14


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 15


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 16


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 17


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 18


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 19


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 20


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 21


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 22


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 23


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 24


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 25


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 26


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 27


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 28


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 29


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 30


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 31


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 32


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 33


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 34


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 35


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 36


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 37


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 38


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 39


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 40


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 41


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 42


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 43


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 44


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 45


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 46


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 47


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 48


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 49


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 50


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 51


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 52


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 53


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 54


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 55


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 56


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 57


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 58


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 59


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 60


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 61


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 62


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 63


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 64


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 65


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 66


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 67


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 68


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 69


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 70


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 71


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 72


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 73


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 74


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 75


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 76


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 77


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 78


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 79


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 80


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 81


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 82


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 83


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 84


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 85


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 86


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 87


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 88


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 89


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 90


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 91


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 92


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 93


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 94


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 95


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 96


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 97


text: ISCIII

text: public

text: S. aureus clinical sample

text: Spain

text: diseased

text: Homo Sapiens

text: Human

text: Staphylococcus aureus

text: Staphylococcus aureus

text: S. aureus sample 98


text: CeNT UW

text: public

text: horse

text: horse

text: Poland

text: Equus caballus

text: Equus caballus


text: CeNT UW

text: public

text: horse

text: horse

text: Serbia

text: Equus caballus

text: Equus caballus


text: Institute of geology UAM

text: public

text: coastal wetland ecosystem

characteristic: broad-scale environmental context

unit: mm

characteristic: depth

text: not collected

characteristic: elevation

text: sand

characteristic: environmental medium

text: Portugal

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Algarve,Martinhal

characteristic: geographic location (region and locality)

text: mudflat

characteristic: local environmental context

text: beach sand metagenome

characteristic: project name

text: spatula

characteristic: sample collection method

text: beach sand metagenome


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Institute of geology UAM

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: coastal wetland ecosystem

characteristic: collection date

text: storm

text: not collected

text: sand

text: Portugal

characteristic: geographic location (country and/or sea)

text: Algarve,Martinhal

text: mudflat

text: sand metagenome

characteristic: organism

text: spatula

text: sand metagenome

characteristic: title


text: Institute of geology UAM

text: public

text: coastal wetland ecosystem

characteristic: broad-scale environmental context

characteristic: depth

text: lagoon

characteristic: description

text: not collected

characteristic: elevation

text: mud

characteristic: environmental medium

text: Portugal

text: Algarve,Martinhal

text: mudflat

text: mud metagenome

text: spatula

text: mud metagenome


text: Institute of geology UAM

text: public

text: coastal wetland ecosystem

text: tsunami

text: not collected

text: sand

text: Portugal

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Algarve,Martinhal

characteristic: geographic location (region and locality)

text: mudflat

characteristic: local environmental context

text: sand metagenome

characteristic: project name

text: spatula

text: sand metagenome


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Institute of geology UAM

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: coastal wetland ecosystem

text: tsunami

text: not collected

text: mud

text: Portugal

text: Algarve,Martinhal

text: mudflat

text: mud metagenome

text: spatula

characteristic: sample collection method

text: mud metagenome


text: Institute of geology UAM

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: coastal wetland ecosystem

characteristic: collection date

text: storm

text: not collected

text: silt

text: Portugal

characteristic: geographic location (country and/or sea)

text: Algarve,Martinhal

text: mudflat

text: sand metagenome

characteristic: organism

text: spatula

text: sand metagenome


text: Institute of geology UAM

text: public

text: coastal wetland ecosystem

characteristic: broad-scale environmental context

characteristic: depth

text: lagoon

characteristic: description

text: not collected

characteristic: elevation

text: mud

text: Portugal

text: Algarve,Martinhal

text: mudflat

text: sand metagenome

text: spatula

text: sand metagenome

characteristic: title


text: Institute of geology UAM

text: public

text: coastal wetland ecosystem

text: tsunami

text: not collected

text: sand

characteristic: environmental medium

text: Portugal

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Algarve,Martinhal

characteristic: geographic location (region and locality)

text: mudflat

characteristic: local environmental context

text: mud metagenome

text: spatula

text: mud metagenome


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Institute of geology UAM

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: coastal wetland ecosystem

text: not collected

text: soil

text: Portugal

text: Algarve,Martinhal

text: mudflat

text: soil metagenome

characteristic: project name

text: spatula

text: soil metagenome


text: Institute of geology UAM

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: coastal wetland ecosystem

characteristic: collection date

text: not collected

text: sand

text: Portugal

characteristic: geographic location (country and/or sea)

text: Algarve,Martinhal

text: mudflat

text: sand metagenome

text: spatula

characteristic: sample collection method

text: sand metagenome


text: Institute of geology UAM

text: public

text: aquatic ecosystem

characteristic: broad-scale environmental context

characteristic: depth

characteristic: description

text: not collected

characteristic: elevation

text: sand

text: Portugal

text: Algarve,Martinhal

text: mudflat

text: marine sediment metagenome

characteristic: organism

text: marine sediment metagenome


text: Institute of geology UAM

text: public

text: marine ecosystem

text: not collected

text: sand

characteristic: environmental medium

text: Portugal

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Algarve,Martinhal

characteristic: geographic location (region and locality)

text: marine neritic benthic zone biome

text: marine sediment metagenome

text: marine sediment metagenome

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: LiU

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: barley

characteristic: common name

text: Archaeological seed # 1, dating from 7-8 century recovered at Cruz de la Esquina cueva funeraria, Gran Canaria. Results of sequencing of DNA enriched through exome capture using SeqCap EZ Developer probe pool design 120426_Barley_BEC_D04.EZ (Mascher et al, 2013).

text: Spain

text: Gran Canaria, Canary Islands

text: Hordeum vulgare

text: Hordeum vulgare

text: Cruz de la Esquina Cueva funeraria seed 1