characteristic: ENA-CHECKLIST

text: Wellcome Sanger Institute

characteristic: INSDC center name

text: public

characteristic: SRA accession

text: ArrayExpress

text: not collected

text: human

characteristic: common name

characteristic: description

text: Carnegie stage 19

text: normal

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: hindlimb

text: hindlimb bud

text: Homo sapiens

text: female


status: PUBLIC

characteristic: ENA first public

tag: attribute

text: Wellcome Sanger Institute

text: public

characteristic: INSDC status

characteristic: Submitter Id

text: 6.2 and unknown

unit: week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: not collected

characteristic: collection date

text: human

text: Protocols: The human embryonic samples were collected from medical abortions. The samples were stored in hibernate E medium (Thermo Fisher A1370601) and transported to the research lab for dissection. The limb samples were stored in HypoThermosol at 4 degrees, then were embedded and frozen in OCT on isopentane-dry ice slurry. Nucleic acid was extracted according to the standard protocol of the Spatial 3' v1 protocol Tissue sections were loaded onto Visium chips according to the standard protocol of the Spatial 3' v1 protocol

text: Carnegie stage 18 and 19

characteristic: developmental stage

text: normal

characteristic: disease

text: not collected

characteristic: geographic location (country and/or sea)

text: BRC2218, BRC2217

characteristic: individual

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: hindlimb

characteristic: organism part

text: hindlimb bud

characteristic: sampling site

text: Homo sapiens

text: not available

characteristic: sex

characteristic: title


characteristic: ENA-CHECKLIST

text: Brunel University London

characteristic: INSDC center name

text: public

characteristic: SRA accession

text: ArrayExpress

text: embryonic cell line

text: embryonic stem cell

text: not collected

text: house mouse

characteristic: common name

text: Protocols: Mouse embryonic stem cells (mES) were detached from gelatinesed flasks by addition of trypsin. Cells were disassembled from 3-dimensional gastruloids (initiated from mES cells and biased towards hemato-endothelial cell production) at 144 hours and 216 hours using the dissociation agent TrypLE Express. Cells suspensions were quenched with medium and centrifuged at 400 x g for 5 minutes to pellet for subsequent extraction. For colony forming assays, disassembled gastruloids cells from 144 hours or 216 hours were plated on Mouse Methylcellulose Complete Media (R&D Systems). Cells were first suspended in Iscove’s Modified Dulbecco’s Medium (IMDM, Gibco) with 20% FBS (Gibco) before addition to the methylcellulose medium. Cells were plated in duplicate 35 mm dishes with 2×10^5 cells/plate. Plates were incubated at 37°C and 5% CO2 for 10 days, when colonies were scored. Total RNA was extracted using Trizol (Invitrogen) and purified using Direct-zol RNA Purification Kits (Zymo Research). Library construction was performed by Novogene UK (Cambridge, UK). A minimum of 400 ng (20 ng/μl) of high-quality total RNA (extracted using Trizol, Invitrogen, and purified using Direct-zol RNA Purification Kits, Zymo Research) was supplied for sequencing. Novogene UK constructed cDNA libraries by purification of messenger RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, PCR amplification, and purification.

characteristic: description

text: embryo stage

text: wild type genotype

characteristic: genotype

text: not collected

text: gastruloid culture

characteristic: growth condition

text: not applicable

text: Mus musculus

text: Mus musculus

text: embryoid body

text: MNX1 overexpression

characteristic: phenotype

text: Mus musculus


characteristic: ENA first public

text: Brunel University London

text: public

characteristic: INSDC status

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: embryonic cell line

text: embryonic stem cell

text: not collected

characteristic: collection date

text: house mouse

text: embryo stage

characteristic: developmental stage

text: wild type genotype

text: not collected

text: gastruloid culture

text: not applicable

text: Mus musculus

text: Mus musculus

text: embryoid body

text: empty vector

text: Mus musculus


text: Brunel University London

text: public

text: ArrayExpress

text: embryonic cell line

text: embryonic stem cell

text: not collected

text: house mouse

text: embryo stage

text: wild type genotype

text: not collected

characteristic: geographic location (country and/or sea)

text: normal cell culture

text: not applicable

characteristic: isolate

text: Mus musculus

characteristic: organism

text: Mus musculus

text: embryoid body

characteristic: organism part

text: empty vector

text: Mus musculus


characteristic: ENA-CHECKLIST

text: Brunel University London

characteristic: INSDC center name

text: public

text: ArrayExpress

text: embryonic cell line

text: embryonic stem cell

text: not collected

text: house mouse

text: embryo stage

text: wild type genotype

text: not collected

text: normal cell culture

text: not applicable

text: Mus musculus

text: Mus musculus

text: embryoid body

text: MNX1 overexpression

text: Mus musculus

characteristic: title


status: PRIVATE

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Bos taurus

characteristic: Organism

characteristic: SRA accession

tag: Namespace:ROSLIN INSTITUTE, UNIVERSITY OF EDINBURGH

text: not provided

text: Cattle

characteristic: common name

text: Kenya Boran - East African Zebu

characteristic: description

text: not provided

text: Bos indicus

text: Died

characteristic: outcome

text: Bos taurus

characteristic: scientific name

text: Bos indicus

text: F

characteristic: sex

characteristic: sire

text: Boran cattle

characteristic: trial

type: derived from

Livestock Genetics

Address: International Livestock Research Institute (ILRI) Addis Ababa, Ethiopia

Abdulfatai Tijjani

FirstName: Abdulfatai

LastName: Tijjani


characteristic: ENA-CHECKLIST

text: ROSLIN INSTITUTE, UNIVERSITY OF EDINBURGH

text: public

characteristic: INSDC status

text: Bos taurus

characteristic: Submitter Id

text: not provided

text: Cattle

text: Kenya Boran - East African Zebu

text: not provided

characteristic: geographic location (country and/or sea)

text: Bos indicus

characteristic: organism

text: Died

text: Bos taurus

text: Bos indicus

text: M

text: Unrelated

text: Boran cattle


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: Bos taurus

text: not provided

text: Cattle

text: Kenya Boran - East African Zebu

text: not provided

text: Bos indicus

text: Died

text: Bos taurus

text: Bos indicus

text: F

text: Unrelated

text: Boran cattle

characteristic: title


characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Bos taurus

characteristic: Organism

characteristic: SRA accession

text: not provided

text: Cattle

characteristic: common name

text: Kenya Boran - East African Zebu

text: not provided

text: Bos indicus

text: Died

text: Bos taurus

text: Bos indicus

text: F

text: Boran cattle


text: THE ROSLIN INSTITUTE

text: public

text: Bos taurus

tag: Namespace:THE ROSLIN INSTITUTE

text: Ayao Missohou at Interstate School of Veterinary Science and Medicine of Dakar (EISMV), Senegal

characteristic: collected by

text: not provided

text: Cattle

text: NDama - West African Taurine

characteristic: description

text: not provided

text: Bos indicus

text: Bos taurus

characteristic: scientific name

text: Bos indicus

text: Zebu Maure cattle


characteristic: ENA-CHECKLIST

text: THE ROSLIN INSTITUTE

text: public

characteristic: INSDC status

text: Bos taurus

text: not provided

text: Cattle

text: NDama - West African Taurine

text: not provided

text: Bos taurus

text: Bos taurus

text: Bos taurus

text: N'Dama cattle


text: THE ROSLIN INSTITUTE

text: public

text: Bos taurus

characteristic: Submitter Id

text: Amadou Traore at Environmental Institute for Agricultural Research (INERA), Burkina Faso

text: not provided

text: Cattle

text: Baoule - West African Taurine

text: not provided

characteristic: geographic location (country and/or sea)

text: Bos taurus

text: Bos taurus

text: Bos taurus

text: Baoule cattle


characteristic: ENA-FIRST-PUBLIC

text: THE ROSLIN INSTITUTE

text: public

text: Bos taurus

text: not provided

text: Cattle

text: Djakkore - West African Admixed

text: not provided

text: Bos indicus

characteristic: organism

text: Bos taurus

text: Bos indicus

text: Djakkore cattle


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: THE ROSLIN INSTITUTE

text: public

text: Bos taurus

text: not provided

text: Cattle

text: Gourounsi - West African Admixed

text: not provided

text: Bos taurus

text: Bos taurus

text: Bos taurus

text: Gourounsi cattle


text: THE ROSLIN INSTITUTE

characteristic: INSDC center name

text: public

text: Bos taurus

text: not provided

text: Cattle

text: NDama - West African Taurine

text: not provided

text: Bos taurus

text: Bos taurus

text: Bos taurus

text: Ndama cattle

characteristic: title


text: THE ROSLIN INSTITUTE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Bos taurus

text: not provided

text: Cattle

text: ZebuGobra - West African Zebu

text: not provided

text: Bos indicus

text: Bos taurus

text: Bos indicus

text: Zebu Gobra cattle


text: THE ROSLIN INSTITUTE

text: public

text: Bos taurus

characteristic: Organism

characteristic: SRA accession

text: not provided

text: Cattle

text: ZebuMaure - West African Zebu

text: not provided

text: Bos indicus

text: Bos taurus

text: Bos indicus

text: Zebu Maure cattle


characteristic: ENA first public

text: Artyukova

text: public

text: ena-SAMPLE-TAB-03-05-2022-06:59:19:895-21239

text: A.Yu. Astashenkov

text: intergenic spacer trnH-psbA

text: Russia

text: Tuva, near Tore-Khol Lake

characteristic: geographic location (region and locality)

text: TTK

characteristic: isolate

text: Iris potaninii

text: Iris potaninii

text: plastid:chloroplast


characteristic: ENA-CHECKLIST

text: Artyukova

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characteristic: description

text: Russia

text: Tuva, near Tore-Khol Lake

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text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-03-05-2022-06:59:19:896-21241

characteristic: Submitter Id

text: A.Yu. Astashenkov

text: intergenic spacer trnS-GCU-trnG-UCC

text: Russia

text: Tuva, near Tore-Khol Lake

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text: A.Yu. Astashenkov

text: intergenic spacer trnL-trnF

text: Russia

characteristic: geographic location (country and/or sea)

text: Tuva, near Tore-Khol Lake

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text: Artyukova

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text: D.N. Shaulo & V.M. Doronkin

text: intergenic spacer trnH-psbA

text: Russia

text: Tuva, near Tore-Khol Lake

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text: Artyukova

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text: D.N. Shaulo & V.M. Doronkin

text: intergenic spacer rps4-trnS-GGA

text: Russia

text: Tuva, near Tore-Khol Lake

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text: plastid:chloroplast


text: Artyukova

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text: D.N. Shaulo & V.M. Doronkin

text: intergenic spacer trnS-GCU-trnG-UCC

text: Russia

text: Tuva, near Tore-Khol Lake

text: TTL

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text: plastid:chloroplast


text: Artyukova

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text: ena-SAMPLE-TAB-03-05-2022-06:59:19:896-21246

text: D.N. Shaulo & V.M. Doronkin

text: intergenic spacer trnL-trnF

text: Russia

text: Tuva, near Tore-Khol Lake

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text: Artyukova

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text: ena-SAMPLE-TAB-03-05-2022-06:59:19:896-21247

text: A.Yu. Astashenkov

text: intergenic spacer trnH-psbA

text: Russia

text: Tuva,near Tes River

text: TTR

text: Iris potaninii

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text: plastid:chloroplast


text: Artyukova

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text: ena-SAMPLE-TAB-03-05-2022-06:59:19:896-21248

text: A.Yu. Astashenkov

text: intergenic spacer rps4-trnS-GGA

text: Russia

text: Tuva,near Tes River

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text: Artyukova

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text: ena-SAMPLE-TAB-03-05-2022-06:59:19:896-21249

text: A.Yu. Astashenkov

text: intergenic spacer trnS-GCU-trnG-UCC

text: Russia

text: Tuva,near Tes River

text: TTR

text: Iris potaninii

text: Iris potaninii

text: plastid:chloroplast


text: Artyukova

text: public

text: ena-SAMPLE-TAB-03-05-2022-06:59:19:896-21250

text: A.Yu. Astashenkov

text: intergenic spacer trnL-trnF

text: Russia

text: Tuva,near Tes River

text: TTR

text: Iris potaninii

text: Iris potaninii

text: plastid:chloroplast


text: Artyukova

text: public

text: ena-SAMPLE-TAB-03-05-2022-06:59:19:896-21251

text: E.V. Boltenkov et al.

text: intergenic spacer trnH-psbA

text: Russia

text: Altai Republic, Kosh-Agach, near Low Boguty Lake

text: LBL

text: Iris potaninii

text: Iris potaninii

text: plastid:chloroplast


text: Artyukova

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text: E.V. Boltenkov et al.

text: intergenic spacer rps4-trnS-GGA

text: Russia

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text: Iris potaninii

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text: Artyukova

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text: E.V. Boltenkov et al.

text: intergenic spacer trnS-GCU-trnG-UCC

text: Russia

text: LBL

text: Iris potaninii

text: Iris potaninii

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text: Artyukova

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text: E.V. Boltenkov et al.

text: intergenic spacer trnL-trnF

text: Russia

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text: Iris potaninii

text: Iris potaninii

text: plastid:chloroplast


text: Artyukova

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text: ena-SAMPLE-TAB-03-05-2022-06:59:19:896-21255

text: E.V. Boltenkov et al.

text: intergenic spacer trnH-psbA

text: Russia

text: Altai Republic, Kosh-Agach, near Tashanta

text: Iris potaninii

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text: plastid:chloroplast


text: Artyukova

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text: intergenic spacer rps4-trnS-GGA

text: Russia

text: Iris potaninii

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text: E.V. Boltenkov et al.

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text: Russia

text: Iris potaninii

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text: E.V. Boltenkov et al.

text: intergenic spacer trnL-trnF

text: Russia

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text: Iris potaninii

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text: E.V. Boltenkov et al.

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text: Russia

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text: Russia

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text: Russia

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text: Russia

text: Iris potaninii

text: Iris potaninii

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text: N.V. Shchegoleva

text: intergenic spacer trnH-psbA

text: Russia

text: Iris potaninii

text: Iris potaninii

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text: P.A. Kosachev et al.

text: intergenic spacer trnH-psbA

text: Russia

text: Altai Republic, Kosh-Agach, 6 km north of Tashanta

text: Iris potaninii

text: Iris potaninii

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text: Artyukova

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text: Russia

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text: P.A. Kosachev et al.

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text: R.V. Kamelin et al.

text: intergenic spacer trnH-psbA

text: Russia

text: Altai Republic, Kosh-Agach, the mouth of Sar-Gobo River

text: SGR

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text: intergenic spacer trnH-psbA

text: Russia

text: Altai Republic, Kosh-Agach, near Chagan-Uzun

text: ARC

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