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characteristic: environmental package

text: yes

text: Sweden

characteristic: geographic location (country and/or sea)

text: Kalmar Strait

text: mimarks-survey

text: microbial community [PCO:1000004]

text: marine metagenome

characteristic: organism

text: marine metagenome

text: Illumina MiSeq

text: true

characteristic: title

text: water


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: public

text: 130919:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: brackish water [ENVO:00002019]

text: water

text: yes

characteristic: experiment

text: Sweden

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Kalmar Strait

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial community [PCO:1000004]

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characteristic: nucleic acid extraction

text: marine metagenome

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characteristic: project name

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characteristic: sample material processing

text: marine metagenome

text: Illumina MiSeq

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text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column

text: water

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characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 130920:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

characteristic: broad-scale environmental context

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: depth

text: Coastal metagenome taken 100m from Zostera marina meadow (20 cm above the sediment)

characteristic: description

text: brackish water [ENVO:00002019]

characteristic: environmental medium

text: water

characteristic: environmental package

text: yes

text: Sweden

characteristic: geographic location (country and/or sea)

text: Kalmar Strait

text: mimarks-survey

text: microbial community [PCO:1000004]

text: marine metagenome

characteristic: organism

text: marine metagenome

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characteristic: ENA first public

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text: 130921:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

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text: microbial community [PCO:1000004]

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text: marine metagenome

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text: public

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text: GFBIO

characteristic: broker name

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text: brackish water [ENVO:00002019]

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text: water

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text: yes

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characteristic: geographic location (country and/or sea)

text: Kalmar Strait

text: mimarks-survey

text: microbial community [PCO:1000004]

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characteristic: organism

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text: Illumina MiSeq

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characteristic: ENA first public

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text: public

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text: brackish water [ENVO:00002019]

text: water

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text: Sweden

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Kalmar Strait

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial community [PCO:1000004]

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text: brackish water [ENVO:00002019]

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text: yes

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text: microbial community [PCO:1000004]

text: PowerSoil DNA isolation - DNeasy PowerSoil Pro Kit

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text: public

text: 130925:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: brackish water [ENVO:00002019]

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text: yes

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characteristic: geographic location (latitude)

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text: Kalmar Strait

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characteristic: investigation type

text: microbial community [PCO:1000004]

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text: FWD:CCTACGGGNGGCWGCAG REV:GACTACHVGGGTATCTAATCC

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text: GFBIO

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text: Coastal metagenome taken 15m from Fucus visiculosus meadow (20 cm above the sediment)

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text: yes

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text: microbial community [PCO:1000004]

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characteristic: organism

text: 16S and 18S rRNA amplicon sequencing of coastal microbial communities across the salinity gradient of the Baltic Sea in pelagic, benthic, and biofilm environments

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characteristic: geographic location (latitude)

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text: Kalmar Strait

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text: mimarks-survey

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text: microbial community [PCO:1000004]

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text: brackish water [ENVO:00002019]

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text: brackish water [ENVO:00002019]

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text: brackish water [ENVO:00002019]

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characteristic: organism

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text: yes

text: Denmark

characteristic: geographic location (country and/or sea)

text: Oresund

text: mimarks-survey

text: microbial community [PCO:1000004]

text: marine metagenome

characteristic: organism

text: FWD:CCTACGGGNGGCWGCAG REV:GACTACHVGGGTATCTAATCC

text: marine metagenome

text: Illumina MiSeq

text: true

characteristic: title

text: water


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: public

text: 130945:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Coastal metagenome taken 100m from Fucus visiculosus/serratus meadow (20 cm above the sediment)

text: brackish water [ENVO:00002019]

text: water

text: yes

characteristic: experiment

text: Denmark

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Oresund

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial community [PCO:1000004]

characteristic: local environmental context

characteristic: nucleic acid extraction

text: marine metagenome

characteristic: pcr primers

characteristic: project name

characteristic: run

characteristic: sample material processing

text: marine metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column

text: water

characteristic: water environmental package


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 130946:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

characteristic: broad-scale environmental context

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: depth

characteristic: description

text: brackish water [ENVO:00002019]

characteristic: environmental medium

text: water

characteristic: environmental package

text: yes

text: Denmark

characteristic: geographic location (country and/or sea)

text: Oresund

text: mimarks-survey

text: microbial community [PCO:1000004]

text: marine metagenome

characteristic: organism

text: 16S and 18S rRNA amplicon sequencing of coastal microbial communities across the salinity gradient of the Baltic Sea in pelagic, benthic, and biofilm environments

text: marine metagenome

text: Illumina MiSeq

text: true

characteristic: title

text: water


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: public

text: 130947:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: brackish water [ENVO:00002019]

text: water

text: yes

characteristic: experiment

text: Denmark

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Oresund

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial community [PCO:1000004]

characteristic: local environmental context

characteristic: nucleic acid extraction

text: marine metagenome

characteristic: pcr primers

characteristic: project name

characteristic: run

text: filtered over 0.22 micrometer filters and shockfrozen

characteristic: sample material processing

text: marine metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column

text: water

characteristic: water environmental package


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 130948:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

characteristic: broad-scale environmental context

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: depth

text: Coastal metagenome taken in a Zostera marina meadow (5cm from the plant, 20 cm above the sediment)

characteristic: description

text: brackish water [ENVO:00002019]

characteristic: environmental medium

text: water

characteristic: environmental package

text: yes

text: Germany

characteristic: geographic location (country and/or sea)

text: Bay of Mecklenburg

text: mimarks-survey

text: microbial community [PCO:1000004]

text: marine metagenome

characteristic: organism

text: marine metagenome

text: Illumina MiSeq

text: true

characteristic: title

text: water


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: public

text: 130949:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: temperate marginal sea biome [ENVO:01000856]

text: GFBIO

text: brackish water [ENVO:00002019]

text: water

text: yes

characteristic: experiment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Bay of Mecklenburg

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial community [PCO:1000004]

characteristic: local environmental context

characteristic: nucleic acid extraction

text: marine metagenome

characteristic: pcr primers

characteristic: project name

characteristic: run

characteristic: sample material processing

text: marine metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column

text: water

characteristic: water environmental package


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 130950:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

characteristic: broad-scale environmental context

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: depth

characteristic: description

text: brackish water [ENVO:00002019]

characteristic: environmental medium

text: water

characteristic: environmental package

text: yes

text: Germany

characteristic: geographic location (country and/or sea)

text: Bay of Mecklenburg

text: mimarks-survey

text: microbial community [PCO:1000004]

text: marine metagenome

characteristic: organism

text: marine metagenome

text: Illumina MiSeq

text: true

characteristic: title

text: water


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: public

text: 130951:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Coastal metagenome taken 15m from Zostera marina meadow (20 cm above the sediment)

text: brackish water [ENVO:00002019]

text: water

text: yes

characteristic: experiment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Bay of Mecklenburg

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial community [PCO:1000004]

characteristic: local environmental context

characteristic: nucleic acid extraction

text: marine metagenome

characteristic: pcr primers

characteristic: project name

characteristic: run

characteristic: sample material processing

text: marine metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column

text: water

characteristic: water environmental package


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: IOW Leibniz-Institut fur Ostseeforschung Warnemuende

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 130952:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

characteristic: broad-scale environmental context

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: depth

characteristic: description

text: brackish water [ENVO:00002019]

characteristic: environmental medium

text: water

characteristic: environmental package

text: yes

text: Germany

characteristic: geographic location (country and/or sea)

text: Bay of Mecklenburg

text: mimarks-survey

text: microbial community [PCO:1000004]

text: PowerSoil DNA isolation - DNeasy PowerSoil Pro Kit

text: marine metagenome

characteristic: organism

text: marine metagenome

text: Illumina MiSeq

text: true

characteristic: title

text: water


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: public

text: 130953:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: brackish water [ENVO:00002019]

text: water

text: yes

characteristic: experiment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Bay of Mecklenburg

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial community [PCO:1000004]

characteristic: local environmental context

characteristic: nucleic acid extraction

text: marine metagenome

text: FWD:CCTACGGGNGGCWGCAG REV:GACTACHVGGGTATCTAATCC

characteristic: pcr primers

characteristic: project name

characteristic: run

characteristic: sample material processing

text: marine metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column

text: water

characteristic: water environmental package


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 130954:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

characteristic: broad-scale environmental context

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: depth

text: Coastal metagenome taken 100m from Zostera marina meadow (20 cm above the sediment)

characteristic: description

text: brackish water [ENVO:00002019]

characteristic: environmental medium

text: water

characteristic: environmental package

text: yes

text: Germany

characteristic: geographic location (country and/or sea)

text: Bay of Mecklenburg

text: mimarks-survey

text: microbial community [PCO:1000004]

text: marine metagenome

characteristic: organism

text: marine metagenome

text: Illumina MiSeq

text: true

characteristic: title

text: water


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: public

text: 130955:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: brackish water [ENVO:00002019]

text: water

text: yes

characteristic: experiment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Bay of Mecklenburg

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial community [PCO:1000004]

characteristic: local environmental context

characteristic: nucleic acid extraction

text: marine metagenome

characteristic: pcr primers

text: 16S and 18S rRNA amplicon sequencing of coastal microbial communities across the salinity gradient of the Baltic Sea in pelagic, benthic, and biofilm environments

characteristic: project name

characteristic: run

characteristic: sample material processing

text: marine metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column

text: water

characteristic: water environmental package


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 130956:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

characteristic: broad-scale environmental context

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: depth

characteristic: description

text: brackish water [ENVO:00002019]

characteristic: environmental medium

text: water

characteristic: environmental package

text: yes

text: Germany

characteristic: geographic location (country and/or sea)

text: Bay of Mecklenburg

text: mimarks-survey

text: microbial community [PCO:1000004]

text: marine metagenome

characteristic: organism

text: filtered over 0.22 micrometer filters and shockfrozen

text: marine metagenome

text: Illumina MiSeq

text: true

characteristic: title

text: water


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: public

text: 130957:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: brackish water [ENVO:00002019]

text: water

text: yes

characteristic: experiment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Baltic Sea, Kiel Bay

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial community [PCO:1000004]

characteristic: local environmental context

characteristic: nucleic acid extraction

text: marine metagenome

characteristic: pcr primers

characteristic: project name

characteristic: run

characteristic: sample material processing

text: marine metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column

text: water

characteristic: water environmental package


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 130958:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

characteristic: broad-scale environmental context

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: depth

characteristic: description

text: brackish water [ENVO:00002019]

characteristic: environmental medium

text: water

characteristic: environmental package

text: yes

text: Germany

characteristic: geographic location (country and/or sea)

text: Baltic Sea, Kiel Bay

text: mimarks-survey

text: microbial community [PCO:1000004]

text: marine metagenome

characteristic: organism

text: marine metagenome

text: Illumina MiSeq

text: true

characteristic: title

text: water


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: public

text: 130959:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: temperate marginal sea biome [ENVO:01000856]

text: GFBIO

text: brackish water [ENVO:00002019]

text: water

text: yes

characteristic: experiment

text: Denmark

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Oresund

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial community [PCO:1000004]

characteristic: local environmental context

characteristic: nucleic acid extraction

text: marine metagenome

characteristic: pcr primers

characteristic: project name

characteristic: run

characteristic: sample material processing

text: marine metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column

text: water

characteristic: water environmental package


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 130960:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

characteristic: broad-scale environmental context

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: depth

characteristic: description

text: brackish water [ENVO:00002019]

characteristic: environmental medium

text: water

characteristic: environmental package

text: yes

text: Denmark

characteristic: geographic location (country and/or sea)

text: Oresund

text: mimarks-survey

text: microbial community [PCO:1000004]

text: marine metagenome

characteristic: organism

text: marine metagenome

text: Illumina MiSeq

text: true

characteristic: title

text: water


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: public

text: 130961:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Coastal metagenome taken in a mixed Zostera marina/Ruppia maritima / Characeae meadow (5cm from the plant, 20 cm above the sediment)

text: brackish water [ENVO:00002019]

text: water

text: yes

characteristic: experiment

text: Estonia

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Baltic Sea, Gulf of Finland

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial community [PCO:1000004]

characteristic: local environmental context

characteristic: nucleic acid extraction

text: marine metagenome

characteristic: pcr primers

characteristic: project name

characteristic: run

characteristic: sample material processing

text: marine metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column

text: water

characteristic: water environmental package


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: IOW Leibniz-Institut fur Ostseeforschung Warnemuende

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 130962:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Seagrass leave biofilm from station BV07-A, replicate a

characteristic: description

characteristic: environment (biome)

text: sea grass bed [ENVO:01000059]

characteristic: environment (feature)

text: plant matter [ENVO:01001121]

characteristic: environment (material)

text: host-associated

characteristic: environmental package

text: Yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: Coastal samples

text: host-associated

characteristic: host-associated environmental package

text: mimarks-specimen

text: Samples were thawed and homogenized. A subsample of 4-6 leaves was transferred into bead-beating tube. This was placed on ice and sonicated twice for 7 min and bead beaten for 30 s at 4 m/s. DNA was extracted with PowerSoil DNA isolation - DNeasy PowerSoil Pro Kit

text: biofilm metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: biofilm metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

text: public

text: 130963:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Seagrass leave biofilm from station BV07-A, replicate b

text: sea grass bed [ENVO:01000059]

text: plant matter [ENVO:01001121]

text: host-associated

text: Yes

characteristic: experiment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: host-associated

text: mimarks-specimen

characteristic: investigation type

characteristic: nucleic acid extraction

text: biofilm metagenome

text: FWD:CCTACGGGNGGCWGCAG REV:GACTACHVGGGTATCTAATCC

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 130964:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Seagrass leave biofilm from station BV07-A, replicate c

characteristic: description

characteristic: environment (biome)

text: sea grass bed [ENVO:01000059]

characteristic: environment (feature)

text: plant matter [ENVO:01001121]

characteristic: environment (material)

text: host-associated

characteristic: environmental package

text: Yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: Coastal samples

text: host-associated

characteristic: host-associated environmental package

text: mimarks-specimen

text: biofilm metagenome

characteristic: organism

text: 16S and 18S rRNA amplicon sequencing of coastal microbial communities across the salinity gradient of the Baltic Sea in pelagic, benthic, and biofilm environments

text: B

text: Stored at -80 C

text: biofilm metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

text: public

text: 130965:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Seagrass leave biofilm from station BV24-A, replicate a

text: sea grass bed [ENVO:01000059]

text: plant matter [ENVO:01001121]

text: host-associated

text: Yes

characteristic: experiment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: host-associated

text: mimarks-specimen

characteristic: investigation type

characteristic: nucleic acid extraction

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 130966:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Seagrass leave biofilm from station BV24-A, replicate b

characteristic: description

characteristic: environment (biome)

text: sea grass bed [ENVO:01000059]

characteristic: environment (feature)

text: plant matter [ENVO:01001121]

characteristic: environment (material)

text: host-associated

characteristic: environmental package

text: Yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: Coastal samples

text: host-associated

characteristic: host-associated environmental package

text: mimarks-specimen

text: biofilm metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: biofilm metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

text: public

text: 130967:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Seagrass leave biofilm from station BV24-A, replicate c

text: temperate marginal sea biome [ENVO:01000856]

text: sea grass bed [ENVO:01000059]

text: plant matter [ENVO:01001121]

text: host-associated

text: Yes

characteristic: experiment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: host-associated

text: mimarks-specimen

characteristic: investigation type

characteristic: nucleic acid extraction

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: IOW Leibniz-Institut fur Ostseeforschung Warnemuende

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 130968:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Seagrass leave biofilm from station BV08-A, replicate a

characteristic: description

characteristic: environment (biome)

text: sea grass bed [ENVO:01000059]

characteristic: environment (feature)

text: plant matter [ENVO:01001121]

characteristic: environment (material)

text: host-associated

characteristic: environmental package

text: Yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: Coastal samples

text: host-associated

characteristic: host-associated environmental package

text: mimarks-specimen

text: biofilm metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: biofilm metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

text: public

text: 130969:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Seagrass leave biofilm from station BV08-A, replicate b

text: sea grass bed [ENVO:01000059]

text: plant matter [ENVO:01001121]

text: host-associated

text: Yes

characteristic: experiment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: host-associated

text: mimarks-specimen

characteristic: investigation type

text: Samples were thawed and homogenized. A subsample of 4-6 leaves was transferred into bead-beating tube. This was placed on ice and sonicated twice for 7 min and bead beaten for 30 s at 4 m/s. DNA was extracted with PowerSoil DNA isolation - DNeasy PowerSoil Pro Kit

characteristic: nucleic acid extraction

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 130970:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Seagrass leave biofilm from station BV08-A, replicate c

characteristic: description

characteristic: environment (biome)

text: sea grass bed [ENVO:01000059]

characteristic: environment (feature)

text: plant matter [ENVO:01001121]

characteristic: environment (material)

text: host-associated

characteristic: environmental package

text: Yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: Coastal samples

text: host-associated

characteristic: host-associated environmental package

text: mimarks-specimen

text: biofilm metagenome

characteristic: organism

text: FWD:CCTACGGGNGGCWGCAG REV:GACTACHVGGGTATCTAATCC

text: B

text: Stored at -80 C

text: biofilm metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

text: public

text: 130971:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Seagrass leave biofilm from station BV10-A, replicate a

text: sea grass bed [ENVO:01000059]

text: plant matter [ENVO:01001121]

text: host-associated

text: Yes

characteristic: experiment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: host-associated

text: mimarks-specimen

characteristic: investigation type

characteristic: nucleic acid extraction

text: biofilm metagenome

characteristic: pcr primers

text: 16S and 18S rRNA amplicon sequencing of coastal microbial communities across the salinity gradient of the Baltic Sea in pelagic, benthic, and biofilm environments

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 130972:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Seagrass leave biofilm from station BV10-A, replicate b

characteristic: description

characteristic: environment (biome)

text: sea grass bed [ENVO:01000059]

characteristic: environment (feature)

text: plant matter [ENVO:01001121]

characteristic: environment (material)

text: host-associated

characteristic: environmental package

text: Yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: Coastal samples

text: host-associated

characteristic: host-associated environmental package

text: mimarks-specimen

text: biofilm metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: biofilm metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

text: public

text: 130973:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Seagrass leave biofilm from station BV10-A, replicate c

text: sea grass bed [ENVO:01000059]

text: plant matter [ENVO:01001121]

text: host-associated

text: Yes

characteristic: experiment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: host-associated

text: mimarks-specimen

characteristic: investigation type

characteristic: nucleic acid extraction

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 130974:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Myriophyllum spicatum material boifilm from station BV-27-A, replicate b

characteristic: description

text: temperate marginal sea biome [ENVO:01000856]

characteristic: environment (biome)

text: plant matter [ENVO:01001121]

characteristic: environment (feature)

text: plant matter [ENVO:01001121]

characteristic: environment (material)

text: host-associated

characteristic: environmental package

text: Yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: Coastal samples

text: host-associated

characteristic: host-associated environmental package

text: mimarks-specimen

text: biofilm metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: biofilm metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: IOW Leibniz-Institut fur Ostseeforschung Warnemuende

characteristic: INSDC center name

text: public

text: 130975:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Myriophyllum spicatum material biofilm from station BV-27-A, replicate c

text: plant matter [ENVO:01001121]

text: plant matter [ENVO:01001121]

text: host-associated

text: Yes

characteristic: experiment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: host-associated

text: mimarks-specimen

characteristic: investigation type

characteristic: nucleic acid extraction

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 130976:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Seagrass leave biofilm from station BV11-A, replicate a

characteristic: description

characteristic: environment (biome)

text: sea grass bed [ENVO:01000059]

characteristic: environment (feature)

text: plant matter [ENVO:01001121]

characteristic: environment (material)

text: host-associated

characteristic: environmental package

text: Yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: Coastal samples

text: host-associated

characteristic: host-associated environmental package

text: mimarks-specimen

text: Samples were thawed and homogenized. A subsample of 4-6 leaves was transferred into bead-beating tube. This was placed on ice and sonicated twice for 7 min and bead beaten for 30 s at 4 m/s. DNA was extracted with PowerSoil DNA isolation - DNeasy PowerSoil Pro Kit

text: biofilm metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: biofilm metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

text: public

text: 130977:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Seagrass leave biofilm from station BV11-A, replicate b

text: sea grass bed [ENVO:01000059]

text: plant matter [ENVO:01001121]

text: host-associated

text: Yes

characteristic: experiment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: host-associated

text: mimarks-specimen

characteristic: investigation type

characteristic: nucleic acid extraction

text: biofilm metagenome

text: FWD:CCTACGGGNGGCWGCAG REV:GACTACHVGGGTATCTAATCC

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 130978:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Seagrass leave biofilm from station BV11-A, replicate c

characteristic: description

characteristic: environment (biome)

text: sea grass bed [ENVO:01000059]

characteristic: environment (feature)

text: plant matter [ENVO:01001121]

characteristic: environment (material)

text: host-associated

characteristic: environmental package

text: Yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: Coastal samples

text: host-associated

characteristic: host-associated environmental package

text: mimarks-specimen

text: biofilm metagenome

characteristic: organism

text: 16S and 18S rRNA amplicon sequencing of coastal microbial communities across the salinity gradient of the Baltic Sea in pelagic, benthic, and biofilm environments

text: B

text: Stored at -80 C

text: biofilm metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

text: public

text: 130979:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Seagrass leave biofilm from station BV05-A, replicate a

text: sea grass bed [ENVO:01000059]

text: plant matter [ENVO:01001121]

text: host-associated

text: Yes

characteristic: experiment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: host-associated

text: mimarks-specimen

characteristic: investigation type

characteristic: nucleic acid extraction

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 130980:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Seagrass leave biofilm from station BV05-A, replicate b

characteristic: description

characteristic: environment (biome)

text: sea grass bed [ENVO:01000059]

characteristic: environment (feature)

text: plant matter [ENVO:01001121]

characteristic: environment (material)

text: host-associated

characteristic: environmental package

text: Yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: Coastal samples

text: host-associated

characteristic: host-associated environmental package

text: mimarks-specimen

text: biofilm metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: biofilm metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

text: public

text: 130981:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Seagrass leave biofilm from station BV05-A, replicate c

text: temperate marginal sea biome [ENVO:01000856]

text: sea grass bed [ENVO:01000059]

text: plant matter [ENVO:01001121]

text: host-associated

text: Yes

characteristic: experiment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: host-associated

text: mimarks-specimen

characteristic: investigation type

characteristic: nucleic acid extraction

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: IOW Leibniz-Institut fur Ostseeforschung Warnemuende

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 130982:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Seagrass leave biofilm from station BV06-A, replicate a

characteristic: description

characteristic: environment (biome)

text: sea grass bed [ENVO:01000059]

characteristic: environment (feature)

text: plant matter [ENVO:01001121]

characteristic: environment (material)

text: host-associated

characteristic: environmental package

text: Yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: Coastal samples

text: host-associated

characteristic: host-associated environmental package

text: mimarks-specimen

text: biofilm metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: biofilm metagenome

text: Illumina MiSeq

text: true

characteristic: title