characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

text: public

text: 130983:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Seagrass leave biofilm from station BV06-A, replicate c

text: sea grass bed [ENVO:01000059]

text: plant matter [ENVO:01001121]

text: host-associated

text: Yes

characteristic: experiment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: host-associated

text: mimarks-specimen

characteristic: investigation type

text: Samples were thawed and homogenized. A subsample of 4-6 leaves was transferred into bead-beating tube. This was placed on ice and sonicated twice for 7 min and bead beaten for 30 s at 4 m/s. DNA was extracted with PowerSoil DNA isolation - DNeasy PowerSoil Pro Kit

characteristic: nucleic acid extraction

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


status: PUBLIC

characteristic: ENA first public

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 130984:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

tag: Namespace:IOW Leibniz-Institut fur Ostseeforschung Warnemuende

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Seagrass leave biofilm from station BV16_2-A, replicate a

characteristic: description

text: temperate marginal sea biome [ENVO:01000856]

characteristic: environment (biome)

text: sea grass bed [ENVO:01000059]

characteristic: environment (feature)

text: plant matter [ENVO:01001121]

characteristic: environment (material)

text: host-associated

characteristic: environmental package

text: Yes

text: Estonia

characteristic: geographic location (country and/or sea)

unit: m

characteristic: geographic location (depth)

unit: DD

text: Coastal samples

text: host-associated

characteristic: host-associated environmental package

text: mimarks-specimen

text: biofilm metagenome

characteristic: organism

text: FWD:CCTACGGGNGGCWGCAG REV:GACTACHVGGGTATCTAATCC

text: 16S and 18S rRNA amplicon sequencing of coastal microbial communities across the salinity gradient of the Baltic Sea in pelagic, benthic, and biofilm environments

text: B

text: Stored at -80 C

text: biofilm metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: IOW Leibniz-Institut fur Ostseeforschung Warnemuende

characteristic: INSDC center name

text: public

text: 130985:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Seagrass leave biofilm from station BV16_2-A, replicate b

text: sea grass bed [ENVO:01000059]

text: plant matter [ENVO:01001121]

text: host-associated

text: Yes

characteristic: experiment

text: Estonia

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: host-associated

text: mimarks-specimen

characteristic: investigation type

characteristic: nucleic acid extraction

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 130986:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Seagrass leave biofilm from station BV16_2-A, replicate c

characteristic: description

characteristic: environment (biome)

text: sea grass bed [ENVO:01000059]

characteristic: environment (feature)

text: plant matter [ENVO:01001121]

characteristic: environment (material)

text: host-associated

characteristic: environmental package

text: Yes

text: Estonia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: Coastal samples

text: host-associated

characteristic: host-associated environmental package

text: mimarks-specimen

text: biofilm metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: biofilm metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

text: public

text: 130987:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Seagrass leave biofilm from station BV16-A, replicate a

text: sea grass bed [ENVO:01000059]

text: plant matter [ENVO:01001121]

text: host-associated

text: Yes

characteristic: experiment

text: Estonia

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: host-associated

text: mimarks-specimen

characteristic: investigation type

characteristic: nucleic acid extraction

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 130988:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Seagrass leave biofilm from station BV16-A, replicate b

characteristic: description

characteristic: environment (biome)

text: sea grass bed [ENVO:01000059]

characteristic: environment (feature)

text: plant matter [ENVO:01001121]

characteristic: environment (material)

text: host-associated

characteristic: environmental package

text: Yes

text: Estonia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: Coastal samples

text: host-associated

characteristic: host-associated environmental package

text: mimarks-specimen

text: biofilm metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: biofilm metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

text: public

text: 130989:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Seagrass leave biofilm from station BV16-A, replicate c

text: sea grass bed [ENVO:01000059]

text: plant matter [ENVO:01001121]

text: host-associated

text: Yes

characteristic: experiment

text: Estonia

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: host-associated

text: mimarks-specimen

characteristic: investigation type

characteristic: nucleic acid extraction

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 130990:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Stuckenia pectinata material from station BV-15-B, replicate a

characteristic: description

characteristic: environment (biome)

text: plant matter [ENVO:01001121]

characteristic: environment (feature)

text: plant matter [ENVO:01001121]

characteristic: environment (material)

text: host-associated

characteristic: environmental package

text: Yes

text: Estonia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: Coastal samples

text: host-associated

characteristic: host-associated environmental package

text: mimarks-specimen

text: Samples were thawed and homogenized. A subsample of 4-6 leaves was transferred into bead-beating tube. This was placed on ice and sonicated twice for 7 min and bead beaten for 30 s at 4 m/s. DNA was extracted with PowerSoil DNA isolation - DNeasy PowerSoil Pro Kit

text: biofilm metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: biofilm metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

text: public

text: 130991:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Stuckenia pectinata material from station BV-15-B, replicate b

text: temperate marginal sea biome [ENVO:01000856]

text: plant matter [ENVO:01001121]

text: plant matter [ENVO:01001121]

text: host-associated

text: Yes

characteristic: experiment

text: Estonia

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: host-associated

text: mimarks-specimen

characteristic: investigation type

characteristic: nucleic acid extraction

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: IOW Leibniz-Institut fur Ostseeforschung Warnemuende

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 130992:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Stuckenia pectinata material from station BV-15-B, replicate c

characteristic: description

characteristic: environment (biome)

text: plant matter [ENVO:01001121]

characteristic: environment (feature)

text: plant matter [ENVO:01001121]

characteristic: environment (material)

text: host-associated

characteristic: environmental package

text: Yes

text: Estonia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: Coastal samples

text: host-associated

characteristic: host-associated environmental package

text: mimarks-specimen

text: biofilm metagenome

characteristic: organism

text: FWD:CCTACGGGNGGCWGCAG REV:GACTACHVGGGTATCTAATCC

text: 16S and 18S rRNA amplicon sequencing of coastal microbial communities across the salinity gradient of the Baltic Sea in pelagic, benthic, and biofilm environments

text: B

text: Stored at -80 C

text: biofilm metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

text: public

text: 130993:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Seagrass leave biofilm from station BV-15-B, replicate b

text: sea grass bed [ENVO:01000059]

text: plant matter [ENVO:01001121]

text: host-associated

text: Yes

characteristic: experiment

text: Estonia

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: host-associated

text: mimarks-specimen

characteristic: investigation type

characteristic: nucleic acid extraction

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 130994:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Stuckenia pectinata material biofilm, BV-15-B, replicate c

characteristic: description

characteristic: environment (biome)

text: plant matter [ENVO:01001121]

characteristic: environment (feature)

text: plant matter [ENVO:01001121]

characteristic: environment (material)

text: host-associated

characteristic: environmental package

text: Yes

text: Estonia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: Coastal samples

text: host-associated

characteristic: host-associated environmental package

text: mimarks-specimen

text: biofilm metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: biofilm metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

text: public

text: 130995:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Seagrass leave biofilm from station BV-15-B, replicate c

text: sea grass bed [ENVO:01000059]

text: plant matter [ENVO:01001121]

text: host-associated

text: Yes

characteristic: experiment

text: Estonia

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: host-associated

text: mimarks-specimen

characteristic: investigation type

characteristic: nucleic acid extraction

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 130996:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Stuckenia pectinata material biofilm from station BV-15-C, replicate a

characteristic: description

characteristic: environment (biome)

text: plant matter [ENVO:01001121]

characteristic: environment (feature)

text: plant matter [ENVO:01001121]

characteristic: environment (material)

text: host-associated

characteristic: environmental package

text: Yes

text: Estonia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: Coastal samples

text: host-associated

characteristic: host-associated environmental package

text: mimarks-specimen

text: biofilm metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: biofilm metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

text: public

text: 130997:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Stuckenia pectinata material biofilm from station BV-15-C, replicate b

text: plant matter [ENVO:01001121]

text: plant matter [ENVO:01001121]

text: host-associated

text: Yes

characteristic: experiment

text: Estonia

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: host-associated

text: mimarks-specimen

characteristic: investigation type

text: Samples were thawed and homogenized. A subsample of 4-6 leaves was transferred into bead-beating tube. This was placed on ice and sonicated twice for 7 min and bead beaten for 30 s at 4 m/s. DNA was extracted with PowerSoil DNA isolation - DNeasy PowerSoil Pro Kit

characteristic: nucleic acid extraction

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 130998:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Stuckenia pectinata material biofilm from station BV-15-C, replicate c

characteristic: description

text: temperate marginal sea biome [ENVO:01000856]

characteristic: environment (biome)

text: plant matter [ENVO:01001121]

characteristic: environment (feature)

text: plant matter [ENVO:01001121]

characteristic: environment (material)

text: host-associated

characteristic: environmental package

text: Yes

text: Estonia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: Coastal samples

text: host-associated

characteristic: host-associated environmental package

text: mimarks-specimen

text: biofilm metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: biofilm metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: IOW Leibniz-Institut fur Ostseeforschung Warnemuende

characteristic: INSDC center name

text: public

text: 130999:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Seagrass leave biofilm from station BV13-A, replicate a

text: sea grass bed [ENVO:01000059]

text: plant matter [ENVO:01001121]

text: host-associated

text: Yes

characteristic: experiment

text: Estonia

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: host-associated

text: mimarks-specimen

characteristic: investigation type

characteristic: nucleic acid extraction

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 131000:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Seagrass leave biofilm from station BV13-A, replicate b

characteristic: description

characteristic: environment (biome)

text: sea grass bed [ENVO:01000059]

characteristic: environment (feature)

text: plant matter [ENVO:01001121]

characteristic: environment (material)

text: host-associated

characteristic: environmental package

text: Yes

text: Estonia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: Coastal samples

text: host-associated

characteristic: host-associated environmental package

text: mimarks-specimen

text: biofilm metagenome

characteristic: organism

text: FWD:CCTACGGGNGGCWGCAG REV:GACTACHVGGGTATCTAATCC

text: 16S and 18S rRNA amplicon sequencing of coastal microbial communities across the salinity gradient of the Baltic Sea in pelagic, benthic, and biofilm environments

text: B

text: Stored at -80 C

text: biofilm metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

text: public

text: 131001:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Seagrass leave biofilm from station BV13-A, replicate c

text: sea grass bed [ENVO:01000059]

text: plant matter [ENVO:01001121]

text: host-associated

text: Yes

characteristic: experiment

text: Estonia

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: host-associated

text: mimarks-specimen

characteristic: investigation type

characteristic: nucleic acid extraction

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 131002:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: description

characteristic: environment (biome)

text: sea grass bed [ENVO:01000059]

characteristic: environment (feature)

text: plant matter [ENVO:01001121]

characteristic: environment (material)

text: host-associated

characteristic: environmental package

text: Yes

text: Estonia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: Coastal samples

text: host-associated

characteristic: host-associated environmental package

text: mimarks-specimen

text: biofilm metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: biofilm metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

text: public

text: 131003:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: sea grass bed [ENVO:01000059]

text: plant matter [ENVO:01001121]

text: host-associated

text: Yes

characteristic: experiment

text: Estonia

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: host-associated

text: mimarks-specimen

characteristic: investigation type

characteristic: nucleic acid extraction

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 131004:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: description

characteristic: environment (biome)

text: sea grass bed [ENVO:01000059]

characteristic: environment (feature)

text: plant matter [ENVO:01001121]

characteristic: environment (material)

text: host-associated

characteristic: environmental package

text: Yes

text: Estonia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: Coastal samples

text: host-associated

characteristic: host-associated environmental package

text: mimarks-specimen

text: biofilm metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: biofilm metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

text: public

text: 131005:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Stuckenia pectinata material biofilm from station BV-13-B, replicate a

text: plant matter [ENVO:01001121]

text: plant matter [ENVO:01001121]

text: host-associated

text: Yes

characteristic: experiment

text: Estonia

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: host-associated

text: mimarks-specimen

characteristic: investigation type

characteristic: nucleic acid extraction

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 131006:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Stuckenia pectinata material biofilm from station BV-13-B, replicate b

characteristic: description

characteristic: environment (biome)

text: plant matter [ENVO:01001121]

characteristic: environment (feature)

text: plant matter [ENVO:01001121]

characteristic: environment (material)

text: host-associated

characteristic: environmental package

text: Yes

text: Estonia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: Coastal samples

text: host-associated

characteristic: host-associated environmental package

text: mimarks-specimen

text: Samples were thawed and homogenized. A subsample of 4-6 leaves was transferred into bead-beating tube. This was placed on ice and sonicated twice for 7 min and bead beaten for 30 s at 4 m/s. DNA was extracted with PowerSoil DNA isolation - DNeasy PowerSoil Pro Kit

text: biofilm metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: biofilm metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: IOW Leibniz-Institut fur Ostseeforschung Warnemuende

characteristic: INSDC center name

text: public

text: 131007:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Stuckenia pectinata material biofilm from station BV-13-B, replicate c

text: temperate marginal sea biome [ENVO:01000856]

text: plant matter [ENVO:01001121]

text: plant matter [ENVO:01001121]

text: host-associated

text: Yes

characteristic: experiment

text: Estonia

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: host-associated

text: mimarks-specimen

characteristic: investigation type

characteristic: nucleic acid extraction

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 131008:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Stuckenia pectinata material biofilm from station BV-13-C, replicate a

characteristic: description

characteristic: environment (biome)

text: plant matter [ENVO:01001121]

characteristic: environment (feature)

text: plant matter [ENVO:01001121]

characteristic: environment (material)

text: host-associated

characteristic: environmental package

text: Yes

text: Estonia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: Coastal samples

text: host-associated

characteristic: host-associated environmental package

text: mimarks-specimen

text: biofilm metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: biofilm metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

text: public

text: 131009:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Stuckenia pectinata material biofilm from station BV-13-C, replicate b

text: plant matter [ENVO:01001121]

text: plant matter [ENVO:01001121]

text: host-associated

text: Yes

characteristic: experiment

text: Estonia

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: host-associated

text: mimarks-specimen

characteristic: investigation type

characteristic: nucleic acid extraction

text: biofilm metagenome

text: FWD:CCTACGGGNGGCWGCAG REV:GACTACHVGGGTATCTAATCC

characteristic: pcr primers

text: 16S and 18S rRNA amplicon sequencing of coastal microbial communities across the salinity gradient of the Baltic Sea in pelagic, benthic, and biofilm environments

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 131010:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Stuckenia pectinata material biofilm from station BV-13-C, replicate c

characteristic: description

characteristic: environment (biome)

text: plant matter [ENVO:01001121]

characteristic: environment (feature)

text: plant matter [ENVO:01001121]

characteristic: environment (material)

text: host-associated

characteristic: environmental package

text: Yes

text: Estonia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: Coastal samples

text: host-associated

characteristic: host-associated environmental package

text: mimarks-specimen

text: biofilm metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: biofilm metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

text: public

text: 131011:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Seagrass leave biofilm from station BV18-A, replicate a

text: sea grass bed [ENVO:01000059]

text: plant matter [ENVO:01001121]

text: host-associated

text: Yes

characteristic: experiment

text: Finland

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: host-associated

text: mimarks-specimen

characteristic: investigation type

characteristic: nucleic acid extraction

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 131012:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Seagrass leave biofilm from station BV18-A, replicate b

characteristic: description

characteristic: environment (biome)

text: sea grass bed [ENVO:01000059]

characteristic: environment (feature)

text: plant matter [ENVO:01001121]

characteristic: environment (material)

text: host-associated

characteristic: environmental package

text: Yes

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: Coastal samples

text: host-associated

characteristic: host-associated environmental package

text: mimarks-specimen

text: biofilm metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: biofilm metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

text: public

text: 131013:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Seagrass leave biofilm from station BV18-A, replicate c

text: sea grass bed [ENVO:01000059]

text: plant matter [ENVO:01001121]

text: host-associated

text: Yes

characteristic: experiment

text: Finland

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: host-associated

text: mimarks-specimen

characteristic: investigation type

text: Samples were thawed and homogenized. A subsample of 4-6 leaves was transferred into bead-beating tube. This was placed on ice and sonicated twice for 7 min and bead beaten for 30 s at 4 m/s. DNA was extracted with PowerSoil DNA isolation - DNeasy PowerSoil Pro Kit

characteristic: nucleic acid extraction

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: IOW Leibniz-Institut fur Ostseeforschung Warnemuende

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 131014:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Seagrass leave biofilm from station BV09-A, replicate b

characteristic: description

characteristic: environment (biome)

text: sea grass bed [ENVO:01000059]

characteristic: environment (feature)

text: plant matter [ENVO:01001121]

characteristic: environment (material)

text: host-associated

characteristic: environmental package

text: Yes

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: Coastal samples

text: host-associated

characteristic: host-associated environmental package

text: mimarks-specimen

text: biofilm metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: biofilm metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

text: public

text: 131015:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Seagrass leave biofilm from station BV09-A, replicate c

text: temperate marginal sea biome [ENVO:01000856]

text: sea grass bed [ENVO:01000059]

text: plant matter [ENVO:01001121]

text: host-associated

text: Yes

characteristic: experiment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: host-associated

text: mimarks-specimen

characteristic: investigation type

characteristic: nucleic acid extraction

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 131016:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Seagrass leave biofilm from station BV14-A, replicate a

characteristic: description

characteristic: environment (biome)

text: sea grass bed [ENVO:01000059]

characteristic: environment (feature)

text: plant matter [ENVO:01001121]

characteristic: environment (material)

text: host-associated

characteristic: environmental package

text: Yes

text: Estonia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: Coastal samples

text: host-associated

characteristic: host-associated environmental package

text: mimarks-specimen

text: biofilm metagenome

characteristic: organism

text: FWD:CCTACGGGNGGCWGCAG REV:GACTACHVGGGTATCTAATCC

text: B

text: Stored at -80 C

text: biofilm metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

text: public

text: 131017:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Seagrass leave biofilm from station BV14-A, replicate b

text: sea grass bed [ENVO:01000059]

text: plant matter [ENVO:01001121]

text: host-associated

text: Yes

characteristic: experiment

text: Estonia

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: host-associated

text: mimarks-specimen

characteristic: investigation type

characteristic: nucleic acid extraction

text: biofilm metagenome

characteristic: pcr primers

text: 16S and 18S rRNA amplicon sequencing of coastal microbial communities across the salinity gradient of the Baltic Sea in pelagic, benthic, and biofilm environments

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 131018:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Seagrass leave biofilm from station BV14-A, replicate c

characteristic: description

characteristic: environment (biome)

text: sea grass bed [ENVO:01000059]

characteristic: environment (feature)

text: plant matter [ENVO:01001121]

characteristic: environment (material)

text: host-associated

characteristic: environmental package

text: Yes

text: Estonia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: Coastal samples

text: host-associated

characteristic: host-associated environmental package

text: mimarks-specimen

text: biofilm metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: biofilm metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

text: public

text: 131019:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Seagrass leave biofilm from station BV12-A, replicate a

text: sea grass bed [ENVO:01000059]

text: plant matter [ENVO:01001121]

text: host-associated

text: Yes

characteristic: experiment

text: Poland

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Bay of Gdansk

characteristic: geographic location (region and locality)

text: host-associated

text: mimarks-specimen

characteristic: investigation type

characteristic: nucleic acid extraction

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 131020:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Seagrass leave biofilm from station BV12-A, replicate b

characteristic: description

characteristic: environment (biome)

text: sea grass bed [ENVO:01000059]

characteristic: environment (feature)

text: plant matter [ENVO:01001121]

characteristic: environment (material)

text: host-associated

characteristic: environmental package

text: Yes

text: Poland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: Bay of Gdansk

text: host-associated

characteristic: host-associated environmental package

text: mimarks-specimen

text: Samples were thawed and homogenized. A subsample of 4-6 leaves was transferred into bead-beating tube. This was placed on ice and sonicated twice for 7 min and bead beaten for 30 s at 4 m/s. DNA was extracted with PowerSoil DNA isolation - DNeasy PowerSoil Pro Kit

text: biofilm metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: biofilm metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: IOW Leibniz-Institut fur Ostseeforschung Warnemuende

characteristic: INSDC center name

text: public

text: 131021:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Seagrass leave biofilm from station BV12-A, replicate c

text: sea grass bed [ENVO:01000059]

text: plant matter [ENVO:01001121]

text: host-associated

text: Yes

characteristic: experiment

text: Poland

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Bay of Gdansk

characteristic: geographic location (region and locality)

text: host-associated

text: mimarks-specimen

characteristic: investigation type

characteristic: nucleic acid extraction

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 131022:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Chara spp. material biofilm from station BV-29-A, replicate a

characteristic: description

text: temperate marginal sea biome [ENVO:01000856]

characteristic: environment (biome)

text: plant matter [ENVO:01001121]

characteristic: environment (feature)

text: plant matter [ENVO:01001121]

characteristic: environment (material)

text: host-associated

characteristic: environmental package

text: Yes

text: Lithuania

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: Coastal samples

text: host-associated

characteristic: host-associated environmental package

text: mimarks-specimen

text: biofilm metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: biofilm metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

text: public

text: 131023:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Chara spp. material biofilm from station BV-29-A, replicate b

text: plant matter [ENVO:01001121]

text: plant matter [ENVO:01001121]

text: host-associated

text: Yes

characteristic: experiment

text: Lithuania

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: host-associated

text: mimarks-specimen

characteristic: investigation type

characteristic: nucleic acid extraction

text: biofilm metagenome

text: FWD:CCTACGGGNGGCWGCAG REV:GACTACHVGGGTATCTAATCC

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 131024:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Chara spp. material biofilm from station BV-29-A, replicate c

characteristic: description

characteristic: environment (biome)

text: plant matter [ENVO:01001121]

characteristic: environment (feature)

text: plant matter [ENVO:01001121]

characteristic: environment (material)

text: host-associated

characteristic: environmental package

text: Yes

text: Lithuania

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: Coastal samples

text: host-associated

characteristic: host-associated environmental package

text: mimarks-specimen

text: biofilm metagenome

characteristic: organism

text: 16S and 18S rRNA amplicon sequencing of coastal microbial communities across the salinity gradient of the Baltic Sea in pelagic, benthic, and biofilm environments

text: B

text: Stored at -80 C

text: biofilm metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

text: public

text: 131025:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Seagrass leave biofilm from station BV21-A, replicate a

text: sea grass bed [ENVO:01000059]

text: plant matter [ENVO:01001121]

text: host-associated

text: Yes

characteristic: experiment

text: Sweden

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: host-associated

text: mimarks-specimen

characteristic: investigation type

characteristic: nucleic acid extraction

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 131026:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Seagrass leave biofilm from station BV21-A, replicate b

characteristic: description

characteristic: environment (biome)

text: sea grass bed [ENVO:01000059]

characteristic: environment (feature)

text: plant matter [ENVO:01001121]

characteristic: environment (material)

text: host-associated

characteristic: environmental package

text: Yes

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: Coastal samples

text: host-associated

characteristic: host-associated environmental package

text: mimarks-specimen

text: biofilm metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: biofilm metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

text: public

text: 131027:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Seagrass leave biofilm from station BV21-A, replicate c

text: sea grass bed [ENVO:01000059]

text: plant matter [ENVO:01001121]

text: host-associated

text: Yes

characteristic: experiment

text: Sweden

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: host-associated

text: mimarks-specimen

characteristic: investigation type

text: Samples were thawed and homogenized. A subsample of 4-6 leaves was transferred into bead-beating tube. This was placed on ice and sonicated twice for 7 min and bead beaten for 30 s at 4 m/s. DNA was extracted with PowerSoil DNA isolation - DNeasy PowerSoil Pro Kit

characteristic: nucleic acid extraction

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: IOW Leibniz-Institut fur Ostseeforschung Warnemuende

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 131028:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Seagrass leave biofilm from station BV20-A, replicate c

characteristic: description

characteristic: environment (biome)

text: sea grass bed [ENVO:01000059]

characteristic: environment (feature)

text: plant matter [ENVO:01001121]

characteristic: environment (material)

text: host-associated

characteristic: environmental package

text: Yes

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: Coastal samples

text: host-associated

characteristic: host-associated environmental package

text: mimarks-specimen

text: biofilm metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: biofilm metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

text: public

text: 131029:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Seagrass leave biofilm from station BV22-A, replicate a

text: temperate marginal sea biome [ENVO:01000856]

text: sea grass bed [ENVO:01000059]

text: plant matter [ENVO:01001121]

text: host-associated

text: Yes

characteristic: experiment

text: Denmark

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: host-associated

text: mimarks-specimen

characteristic: investigation type

characteristic: nucleic acid extraction

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 131030:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Seagrass leave biofilm from station BV22-A, replicate b

characteristic: description

characteristic: environment (biome)

text: sea grass bed [ENVO:01000059]

characteristic: environment (feature)

text: plant matter [ENVO:01001121]

characteristic: environment (material)

text: host-associated

characteristic: environmental package

text: Yes

text: Denmark

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: Coastal samples

text: host-associated

characteristic: host-associated environmental package

text: mimarks-specimen

text: biofilm metagenome

characteristic: organism

text: FWD:CCTACGGGNGGCWGCAG REV:GACTACHVGGGTATCTAATCC

text: B

text: Stored at -80 C

text: biofilm metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

text: public

text: 131031:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: broad-scale environmental context

text: GFBIO

characteristic: depth

text: Coastal sediment metagenome taken in a Zostera marina meadow

characteristic: elevation

text: shallow marine sediment [ENVO:03000034]

characteristic: environmental medium

text: sediment

text: yes

characteristic: experiment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial community [PCO:1000004]

characteristic: local environmental context

text: Samples were thawed and homogenized. A subsample of 500 mg of sediment was transferred into bead-beating tube. This was placed on ice and sonicated twice for 7 min and bead beaten for 30 s at 4 m/s. DNA was extracted with PowerSoil DNA isolation - DNeasy PowerSoil Pro Kit

characteristic: nucleic acid extraction

text: marine sediment metagenome

characteristic: pcr primers

text: 16S and 18S rRNA amplicon sequencing of coastal microbial communities across the salinity gradient of the Baltic Sea in pelagic, benthic, and biofilm environments

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: marine sediment metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 131032:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

characteristic: broad-scale environmental context

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: elevation

text: shallow marine sediment [ENVO:03000034]

characteristic: environmental medium

text: sediment

characteristic: environmental package

text: yes

text: Germany

characteristic: geographic location (country and/or sea)

text: Coastal samples

text: mimarks-survey

text: microbial community [PCO:1000004]

text: marine sediment metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: marine sediment metagenome

text: sediment

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

text: 131033:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: shallow marine sediment [ENVO:03000034]

text: sediment

text: yes

characteristic: experiment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial community [PCO:1000004]

characteristic: local environmental context

characteristic: nucleic acid extraction

text: marine sediment metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: marine sediment metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 131034:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

characteristic: broad-scale environmental context

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: depth

text: Coastal sediment metagenome taken 15m from a Zostera marina meadow

characteristic: description

characteristic: elevation

text: shallow marine sediment [ENVO:03000034]

characteristic: environmental medium

text: sediment

characteristic: environmental package

text: yes

text: Germany

characteristic: geographic location (country and/or sea)

text: Coastal samples

text: mimarks-survey

text: microbial community [PCO:1000004]

text: marine sediment metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: marine sediment metagenome

text: sediment

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: IOW Leibniz-Institut fur Ostseeforschung Warnemuende

text: public

text: 131035:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: shallow marine sediment [ENVO:03000034]

text: sediment

text: yes

characteristic: experiment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial community [PCO:1000004]

characteristic: local environmental context

characteristic: nucleic acid extraction

text: marine sediment metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: marine sediment metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 131036:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

characteristic: broad-scale environmental context

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: depth

text: Coastal sediment metagenome taken 100m from a Zostera marina meadow

characteristic: description

characteristic: elevation

text: shallow marine sediment [ENVO:03000034]

characteristic: environmental medium

text: sediment

characteristic: environmental package

text: yes

text: Germany

characteristic: geographic location (country and/or sea)

text: Coastal samples

text: mimarks-survey

text: microbial community [PCO:1000004]

text: marine sediment metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: marine sediment metagenome

text: sediment

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

text: 131037:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: shallow marine sediment [ENVO:03000034]

text: sediment

text: yes

characteristic: experiment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial community [PCO:1000004]

characteristic: local environmental context

characteristic: nucleic acid extraction

text: marine sediment metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: marine sediment metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 131038:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

text: temperate marginal sea biome [ENVO:01000856]

characteristic: broad-scale environmental context

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: elevation

text: shallow marine sediment [ENVO:03000034]

characteristic: environmental medium

text: sediment

characteristic: environmental package

text: yes

text: Germany

characteristic: geographic location (country and/or sea)

text: Coastal samples

text: mimarks-survey

text: microbial community [PCO:1000004]

text: marine sediment metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: marine sediment metagenome

text: sediment

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

text: 131039:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: shallow marine sediment [ENVO:03000034]

text: sediment

text: yes

characteristic: experiment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial community [PCO:1000004]

characteristic: local environmental context

characteristic: nucleic acid extraction

text: marine sediment metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: marine sediment metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 131040:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

characteristic: broad-scale environmental context

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: elevation

text: shallow marine sediment [ENVO:03000034]

characteristic: environmental medium

text: sediment

characteristic: environmental package

text: yes

text: Germany

characteristic: geographic location (country and/or sea)

text: Coastal samples

text: mimarks-survey

text: microbial community [PCO:1000004]

text: marine sediment metagenome

characteristic: organism

text: FWD:CCTACGGGNGGCWGCAG REV:GACTACHVGGGTATCTAATCC

text: B

text: Stored at -80 C

text: marine sediment metagenome

text: sediment

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

text: 131041:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Coastal sediment metagenome taken in a Zostera marina meadow

text: shallow marine sediment [ENVO:03000034]

text: sediment

text: yes

characteristic: experiment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial community [PCO:1000004]

characteristic: local environmental context

characteristic: nucleic acid extraction

text: marine sediment metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: marine sediment metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 131042:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

characteristic: broad-scale environmental context

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: elevation

text: shallow marine sediment [ENVO:03000034]

characteristic: environmental medium

text: sediment

characteristic: environmental package

text: yes

text: Germany

characteristic: geographic location (country and/or sea)

text: Coastal samples

text: mimarks-survey

text: microbial community [PCO:1000004]

text: Samples were thawed and homogenized. A subsample of 500 mg of sediment was transferred into bead-beating tube. This was placed on ice and sonicated twice for 7 min and bead beaten for 30 s at 4 m/s. DNA was extracted with PowerSoil DNA isolation - DNeasy PowerSoil Pro Kit

text: marine sediment metagenome

characteristic: organism

text: 16S and 18S rRNA amplicon sequencing of coastal microbial communities across the salinity gradient of the Baltic Sea in pelagic, benthic, and biofilm environments

text: B

text: Stored at -80 C

text: marine sediment metagenome

text: sediment

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

text: 131043:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: shallow marine sediment [ENVO:03000034]

text: sediment

text: yes

characteristic: experiment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial community [PCO:1000004]

characteristic: local environmental context

characteristic: nucleic acid extraction

text: marine sediment metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: marine sediment metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 131044:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

characteristic: broad-scale environmental context

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: depth

text: Coastal sediment metagenome taken 15m from a Zostera marina meadow

characteristic: description

characteristic: elevation

text: shallow marine sediment [ENVO:03000034]

characteristic: environmental medium

text: sediment

characteristic: environmental package

text: yes

text: Germany

characteristic: geographic location (country and/or sea)

text: Coastal samples

text: mimarks-survey

text: microbial community [PCO:1000004]

text: marine sediment metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: marine sediment metagenome

text: sediment

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: IOW Leibniz-Institut fur Ostseeforschung Warnemuende

text: public

text: 131045:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: shallow marine sediment [ENVO:03000034]

text: sediment

text: yes

characteristic: experiment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial community [PCO:1000004]

characteristic: local environmental context

characteristic: nucleic acid extraction

text: marine sediment metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: marine sediment metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 131046:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

characteristic: broad-scale environmental context

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: depth

text: Coastal sediment metagenome taken 100m from a Zostera marina meadow

characteristic: description

characteristic: elevation

text: shallow marine sediment [ENVO:03000034]

characteristic: environmental medium

text: sediment

characteristic: environmental package

text: yes

text: Germany

characteristic: geographic location (country and/or sea)

text: Coastal samples

text: mimarks-survey

text: microbial community [PCO:1000004]

text: marine sediment metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: marine sediment metagenome

text: sediment

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

text: 131047:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: shallow marine sediment [ENVO:03000034]

text: sediment

text: yes

characteristic: experiment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial community [PCO:1000004]

characteristic: local environmental context

characteristic: nucleic acid extraction

text: marine sediment metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: marine sediment metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 131048:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

text: temperate marginal sea biome [ENVO:01000856]

characteristic: broad-scale environmental context

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: elevation

text: shallow marine sediment [ENVO:03000034]

characteristic: environmental medium

text: sediment

characteristic: environmental package

text: yes

text: Germany

characteristic: geographic location (country and/or sea)

text: Coastal samples

text: mimarks-survey

text: microbial community [PCO:1000004]

text: marine sediment metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: marine sediment metagenome

text: sediment

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

text: 131049:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: shallow marine sediment [ENVO:03000034]

text: sediment

text: yes

characteristic: experiment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial community [PCO:1000004]

characteristic: local environmental context

characteristic: nucleic acid extraction

text: marine sediment metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: marine sediment metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 131050:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

characteristic: broad-scale environmental context

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: elevation

text: shallow marine sediment [ENVO:03000034]

characteristic: environmental medium

text: sediment

characteristic: environmental package

text: yes

text: Germany

characteristic: geographic location (country and/or sea)

text: Coastal samples

text: mimarks-survey

text: microbial community [PCO:1000004]

text: marine sediment metagenome

characteristic: organism

text: FWD:CCTACGGGNGGCWGCAG REV:GACTACHVGGGTATCTAATCC

text: B

text: Stored at -80 C

text: marine sediment metagenome

text: sediment

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

text: 131051:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: shallow marine sediment [ENVO:03000034]

text: sediment

text: yes

characteristic: experiment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial community [PCO:1000004]

characteristic: local environmental context

characteristic: nucleic acid extraction

text: marine sediment metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: marine sediment metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 131052:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

characteristic: broad-scale environmental context

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: elevation

text: shallow marine sediment [ENVO:03000034]

characteristic: environmental medium

text: sediment

characteristic: environmental package

text: yes

text: Germany

characteristic: geographic location (country and/or sea)

text: Coastal samples

text: mimarks-survey

text: microbial community [PCO:1000004]

text: marine sediment metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: marine sediment metagenome

text: sediment

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

text: 131053:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: shallow marine sediment [ENVO:03000034]

text: sediment

text: yes

characteristic: experiment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial community [PCO:1000004]

characteristic: local environmental context

text: Samples were thawed and homogenized. A subsample of 500 mg of sediment was transferred into bead-beating tube. This was placed on ice and sonicated twice for 7 min and bead beaten for 30 s at 4 m/s. DNA was extracted with PowerSoil DNA isolation - DNeasy PowerSoil Pro Kit

characteristic: nucleic acid extraction

text: marine sediment metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: marine sediment metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 131054:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

characteristic: broad-scale environmental context

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: depth

text: Coastal sediment metagenome taken in a Zostera marina meadow

characteristic: description

characteristic: elevation

text: shallow marine sediment [ENVO:03000034]

characteristic: environmental medium

text: sediment

characteristic: environmental package

text: yes

text: Germany

characteristic: geographic location (country and/or sea)

text: Coastal samples

text: mimarks-survey

text: microbial community [PCO:1000004]

text: marine sediment metagenome

characteristic: organism

text: 16S and 18S rRNA amplicon sequencing of coastal microbial communities across the salinity gradient of the Baltic Sea in pelagic, benthic, and biofilm environments

text: B

text: Stored at -80 C

text: marine sediment metagenome

text: sediment

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: IOW Leibniz-Institut fur Ostseeforschung Warnemuende

text: public

text: 131055:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: shallow marine sediment [ENVO:03000034]

text: sediment

text: yes

characteristic: experiment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial community [PCO:1000004]

characteristic: local environmental context

characteristic: nucleic acid extraction

text: marine sediment metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: marine sediment metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 131056:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

characteristic: broad-scale environmental context

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: elevation

text: shallow marine sediment [ENVO:03000034]

characteristic: environmental medium

text: sediment

characteristic: environmental package

text: yes

text: Germany

characteristic: geographic location (country and/or sea)

text: Coastal samples

text: mimarks-survey

text: microbial community [PCO:1000004]

text: marine sediment metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: marine sediment metagenome

text: sediment

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

text: 131057:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Coastal sediment metagenome taken 15m from a Zostera marina meadow

text: shallow marine sediment [ENVO:03000034]

text: sediment

text: yes

characteristic: experiment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial community [PCO:1000004]

characteristic: local environmental context

characteristic: nucleic acid extraction

text: marine sediment metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: marine sediment metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 131058:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

characteristic: broad-scale environmental context

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: elevation

text: shallow marine sediment [ENVO:03000034]

characteristic: environmental medium

text: sediment

characteristic: environmental package

text: yes

text: Germany

characteristic: geographic location (country and/or sea)

text: Coastal samples

text: mimarks-survey

text: microbial community [PCO:1000004]

text: marine sediment metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: marine sediment metagenome

text: sediment

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

text: 131059:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: temperate marginal sea biome [ENVO:01000856]

text: GFBIO

text: shallow marine sediment [ENVO:03000034]

text: sediment

text: yes

characteristic: experiment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial community [PCO:1000004]

characteristic: local environmental context

characteristic: nucleic acid extraction

text: marine sediment metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: marine sediment metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 131060:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

characteristic: broad-scale environmental context

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: depth

text: Coastal sediment metagenome taken 100m from a Zostera marina meadow

characteristic: description

characteristic: elevation

text: shallow marine sediment [ENVO:03000034]

characteristic: environmental medium

text: sediment

characteristic: environmental package

text: yes

text: Germany

characteristic: geographic location (country and/or sea)

text: Coastal samples

text: mimarks-survey

text: microbial community [PCO:1000004]

text: marine sediment metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: marine sediment metagenome

text: sediment

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

text: 131061:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: shallow marine sediment [ENVO:03000034]

text: sediment

text: yes

characteristic: experiment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial community [PCO:1000004]

characteristic: local environmental context

characteristic: nucleic acid extraction

text: marine sediment metagenome

text: FWD:CCTACGGGNGGCWGCAG REV:GACTACHVGGGTATCTAATCC

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: marine sediment metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 131062:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

characteristic: broad-scale environmental context

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: elevation

text: shallow marine sediment [ENVO:03000034]

characteristic: environmental medium

text: sediment

characteristic: environmental package

text: yes

text: Germany

characteristic: geographic location (country and/or sea)

text: Coastal samples

text: mimarks-survey

text: microbial community [PCO:1000004]

text: marine sediment metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: marine sediment metagenome

text: sediment

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

text: 131063:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: Coastal sediment metagenome taken in a Potamegaton meadow

text: shallow marine sediment [ENVO:03000034]

text: sediment

text: yes

characteristic: experiment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial community [PCO:1000004]

characteristic: local environmental context

text: Samples were thawed and homogenized. A subsample of 500 mg of sediment was transferred into bead-beating tube. This was placed on ice and sonicated twice for 7 min and bead beaten for 30 s at 4 m/s. DNA was extracted with PowerSoil DNA isolation - DNeasy PowerSoil Pro Kit

characteristic: nucleic acid extraction

text: marine sediment metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: marine sediment metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 131064:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

characteristic: broad-scale environmental context

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: depth

text: Coastal sediment metagenome taken 15m from a Potamegaton meadow

characteristic: description

characteristic: elevation

text: shallow marine sediment [ENVO:03000034]

characteristic: environmental medium

text: sediment

characteristic: environmental package

text: yes

text: Germany

characteristic: geographic location (country and/or sea)

text: Coastal samples

text: mimarks-survey

text: microbial community [PCO:1000004]

text: marine sediment metagenome

characteristic: organism

text: 16S and 18S rRNA amplicon sequencing of coastal microbial communities across the salinity gradient of the Baltic Sea in pelagic, benthic, and biofilm environments

text: B

text: Stored at -80 C

text: marine sediment metagenome

text: sediment

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: IOW Leibniz-Institut fur Ostseeforschung Warnemuende

text: public

text: 131065:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

text: GFBIO

text: shallow marine sediment [ENVO:03000034]

text: sediment

text: yes

characteristic: experiment

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Coastal samples

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial community [PCO:1000004]

characteristic: local environmental context

characteristic: nucleic acid extraction

text: marine sediment metagenome

characteristic: pcr primers

characteristic: project name

text: B

characteristic: run

text: Stored at -80 C

characteristic: sample material processing

text: marine sediment metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 131066:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67

characteristic: Submitter Id

characteristic: broad-scale environmental context

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: depth

characteristic: description

characteristic: elevation

text: shallow marine sediment [ENVO:03000034]

characteristic: environmental medium

text: sediment

characteristic: environmental package

text: yes

text: Germany

characteristic: geographic location (country and/or sea)

text: Coastal samples

text: mimarks-survey

text: microbial community [PCO:1000004]

text: marine sediment metagenome

characteristic: organism

text: B

text: Stored at -80 C

text: marine sediment metagenome

text: sediment

text: Illumina MiSeq

text: true

characteristic: title