E-MTAB-13687:scRNA Sample 13_hashed_hto

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: CNAG-CRG

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-13687:scRNA Sample 13_hashed_hto

text: ArrayExpress

text: All

text: validation

text: not collected

text: human

text: adult

characteristic: developmental stage

text: sleep apnea

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: palatine tonsil

text: 10x 3' v3

text: Homo sapiens

text: male

text: scRNA Sample 13_hashed_hto


E-MTAB-13687:scRNA Sample 15_hashed_hto

status: PUBLIC

tag: attribute

text: CNAG-CRG

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13687:scRNA Sample 15_hashed_hto

characteristic: Submitter Id

unit: year

characteristic: age

text: ArrayExpress

characteristic: broker name

text: All

text: discovery

characteristic: cohort

text: not collected

characteristic: collection date

text: human

characteristic: common name

text: Protocols: We divided the sample collection into two cohorts: the discovery cohort (which we used to cluster cells, annotate cell types and identify bona-fide markers), and the validation cohort (which we used to validate cell types and markers). For the discovery cohort, we obtained ten tonsil samples from donors from three different age groups, i.e., six children (age 3-5; 3 males and 3 females; recurrent tonsillitis), three young adults (age 26-35; all male; sleep apnea) and one old adult (age 65; male, tonsil removed during surgery for benign pharyngeal squamous papillomatosis (Figure 1A and Table S1). Out of the ten tonsils, eight were obtained in Clinica Universidad de Navarra (Pamplona, Spain; all kids and 2 young adults) and two at Hospital Clinic Barcelona (Barcelona, Spain; one young and one old adult). All donors or legal guardians gave informed consent for their participation in this study, which was approved by the clinical research ethics committee of Clínica Universidad de Navarra and by the clinical research ethics committee of the Hospital Clinic of Barcelona (HCB/2018/0992). For the validation cohort, we obtained four tonsils from young adults undergoing elective tonsillectomies for recurrent tonsillitis at Newcastle Upon Tyne Hospitals NHS Foundation Trust (United Kingdom). All donors provided written informed consent for study participation, which was approved by the West of Scotland Research Ethics Service (22/WS/0126). Furthermore, the validation cohort included one young adult (age 25; female; recurrent tonsillitis) and two old adults (age 56, 63; both males; sleep apnea and tonsil removed during surgery for superficial squamous carcinoma of the laryngeal vocal cord) sampled at Hospital Clinic after giving their informed consents.All tonsil samples from the discovery and validation cohorts were reviewed at the Hematopathology Unit of Hospital Clinic of Barcelona and showed reactive follicular hyperplasia with several degrees of germinal center expansions. No atypical cells in the epithelium, stroma or lymphoid compartments were seen in the cases including the two tonsils (BCLL-2-T and BCLL-24-T; Table S1) that were extracted in the course of a surgery intervention for benign pharyngeal squamous papillomatosis and superficial squamous carcinoma of vocal cord, respectively.Tonsil tissues were split into three parts that were processed as follows: (1) first part was paraffin embedded to create FFPE blocks, according to standard pathology protocols; (2) second part was snap frozen to obtain OCT blocks, according to standard protocols, and (3) the remaining third part was processed to obtain a single-cell suspension, following the steps described below. Tonsils were first disaggregated by extensive manual mincing and filtered by 70 μm (samples from CIMA and Hospital Clinic) or 100 μm (samples from Newcastle Upone Tyne Hospitals) nylon strainer. In case non- disaggregated parts of tissue were still present, the samples were further dissociated by gentle MACS Dissociator (program tumor 04.01). The number and viability of cells was evaluated. All steps were performed at 4oC or on ice. Cells were either processed directly for single-cell sequencing (scRNA-seq, scATAC-seq, CITE-seq, or Multiome) or cryopreserved for later use.For the final part of the study, cryopreserved cells from tonsil samples of two mantle cell lymphoma (MCL) patients were used (age 64 and 80, both male). Informed consents were obtained according to the Institutional Review Board of the Hospital Clínic of Barcelona following the International Cancer Genome Consortium guidelines. MCL samples were obtained from cryopreserved dissociated cells from tonsils, from the ICGC case collection of Hospital Clinic, Barcelona. After thawing in culture medium supplemented with 20% FBS, the CD19 positive B cell fraction and CD19 negative non-B cell fraction were isolated by magnetic cell separation using CD19-MicroBeads MACS separation system protocol (Miltenyi Biotec, Auburn, CA). Separation steps were performed at 4oC. Both fractions were directly processed for Multiome library preparation and sequenced separately. See nucleic acid library construction protocol for a detailed description of the full protocol from sample extraction, nuclei acid extraction and library preparation. 3’ scRNA-seq and Cell hashing (Hospital Clinic and CIMA) Freshly isolated cells from tonsils were subjected to a Cell Hashing 26 protocol before proceeding to scRNA-seq. Cell hashing was performed following manufacturer’s instructions (Cell hashing and Single Cell Proteogenomics Protocol Using TotalSeqTM Antibodies; BioLegend). Cells were counted with a TC20TM Automated Cell Counter (Bio-Rad Laboratories, S.A), and 50,000 unlabeled cells were saved in a separate tube before proceeding with the cell hashing protocol. Each sample was split into seven aliquots with equal numbers of cells. Briefly, each aliquot was resuspended in Cell Staining Buffer (BioLegend), incubated for 10 min at 4°C with Human TruStain FcXTM Fc Blocking reagent (BioLegend). To each aliquot, a specific TotalSeq-A antibody-oligo conjugate (Table S21) was added and incubated on ice for 30 min. Cells were then washed three times with cold PBS+0.05% BSA (ThermoFisher) and centrifuged for 5 min at 500 rcf at 4°C. Finally, cells were resuspended in an appropriate volume of PBS+0.05% BSA to obtain a final cell concentration >1000 cells/μl, suitable for scRNA-seq. Assuming a 50% loss of cells in all tubes, an equal volume of hashed cell suspension from each of the seven aliquots was mixed and filtered with a 40 μm strainer. Cell concentration was verified with a TC20TM Automated Cell Counter (Bio-Rad Laboratories, S.A) upon cell staining with Trypan Blue. Cells were partitioned into Gel Beads-in-emulsion (GEMs) by using the Chromium Controller system (10X Genomics). Each sample was loaded into two channels with a target recovery of 20,000 cells per channel, for a total final recovery of 40,000 cells per sample. To assess the potential effects of cell hashing on gene expression and cell type composition, we additionally added non-hashed cells as a control (TR=5,000 cells). cDNA sequencing libraries were prepared using the Next GEM Single Cell 3’ Reagent Kits v3.1 (10X Genomics, PN-1000121), with some adaptations for Cell hashing, as indicated in TotalSeqTM-A antibodies and Cell Hashing with 10X Single Cell 3' Reagent Kit v3 3.1 protocol by BioLegend. Briefly, 1 μl of 0.2 μM hashtag oligonucleotides (HTO) primer (Integrated DNA Technologies, IDT) was added to the cDNA amplification reaction to amplify the HTO together with the full-length cDNAs. A SPRI selection clean up was done to separate mRNA-derived cDNA (>300 bp) from antibody-oligo-derived cDNA (<180 bp), as described in the above-mentioned protocol form BioLegend. Gene Expression (GEX) libraries were prepared following 10X Genomics single-cell 3’ mRNA kit protocol, while HTO DNAs were indexed by PCR as follows. 5 μl of purified HTO DNA were mixed with 2.5 μl of 10 μM Illumina TruSeq D70X_s primer (IDT) carrying a different i7 index for each sample (Table S22), 2.5 μl of SI primer from 10X single-cell 3’ mRNA kit, 50 μl of 2 X KAPA HiFi PCR Master Mix (KAPA Biosystem) and 40 μl of nuclease-free water. The reaction was carried out using the following thermal cycling conditions: 98°C for 2 min (initial denaturation), 12 cycles of 98°C for 20 seconds, 64°C for 30 seconds, 72°C for 20 seconds, and a final extension at 72°C for 5 min. The HTO libraries were purified by adding 1.2 X SPRI select reagent to the PCR reaction, incubating 5 min at room temperature (RT) and removing the supernatant after capturing the beads with a magnet. Samples were washed two times with 80% ethanol and elution was performed by adding 40.5 μl of nuclease-free water to the beads. Size distribution and concentration of full-length cDNA and HTO libraries were verified on an Agilent Bioanalyzer High Sensitivity chip (Agilent Technologies). Finally, sequencing of HTO and GEX libraries was carried out on a NovaSeq 6000 sequencer (Illumina) using the following sequencing conditions: 28 bp (Read 1) + 8 bp (i7 index) + 0 bp (i5 index) + 89 bp (Read 2), to obtain approximately 2,000 and >20,000 paired-end reads per HTO and cell, respectively. 3’ scRNA-seq (Newcastle Upon Tyne Hospitals) Fresh cells obtained by FACS sorting were counted, and resuspended in the appropriate volume of PBS to obtain a final cell concentration of 1000 cells/μl for scRNA-seq. Each fresh sample was loaded into two channels of a chromium controller system (10X Genomics) for partitioning into gel beads-in- emulsion (GEMs), with a target recovery of 10,000 cells per channel and 20,000 cells per sample. cDNA libraries were prepared using the Next GEM Single Cell 3’ Reagent Kits v3.1 (10X Genomics, PN- 1000121). Gene expression (GEX) libraries were prepared according to the 10X Genomics single-cell 3’ mRNA kit protocol. Size distribution and concentration of full-length cDNA libraries were quality controlled using a 4200 TapeStation System (Aligent Technologies). GEX libraries were sequenced using a NovaSeq 6000 (Illumina) using the following sequencing conditions: 28 bp (Read 1) + 10 bp (i7 index) + 10 (i5 index) + 90 bp (Read 2) to obtain approximately 25,000 paired-end reads per cell. scATAC-seq Cryopreserved samples were rapidly thawed in a 37oC water bath and transferred to a 15 ml Falcon using a 1000 μl wide bore tip. Next, 1 ml of 37oC pre-warmed Hibernate-A media supplemented with 10% FBS (Thermo Fisher Scientific) was added dropwise while gently swirling the sample. After 1 min RT incubation, 2 ml of pre-warmed media were added as mentioned before. Samples were again incubated at RT for 1 min and then additional media was added to bring the volume to 15 ml. Samples were centrifuged at 500 x g for 5 min at RT. Supernatant was removed and pellets resuspended in 10 ml of 1X PBS (Thermo Fisher Scientific) supplemented with 1% BSA. Samples were filtered with a 40 μm cell strainer to remove clumps. Cell number and viability were verified with a TC20TM Automated Cell Counter (Bio-Rad). Dead cells were removed by FACS sorting DAPI negative cells using a FACSAriaTM Fusion Flow Cytometer (BD Biosciences). To determine potential biases introduced by cell sorting, unsorted cells from 4 out of 10 samples were used for scATAC-seq analysis and compared with corresponding sorted samples. Nuclei isolation was performed following the “Nuclei Isolation for Single Cell ATAC Sequencing” demonstrated protocol (10X Genomics; CG000169) starting from 1 million cells per sample and incubating on ice for 3 min for cell lysis. Based on the starting number of cells and assuming a 50% loss during the procedure, nuclei were resuspended into the appropriate volume of chilled Diluted Nuclei Buffer (10x Genomics) to achieve a nuclei concentration of 925-2300 nuclei/μl, suitable for a Target Nuclei Recovery of 5,000 nuclei per sample. The resulting nuclei concentration was determined by manual counting using a Neubauer chamber upon staining with Trypan Blue. scATAC-seq libraries were prepared according to the Chromium Single Cell ATAC Reagent Kits v1.1 User Guide (10x Genomics; CG000209 Rev D). Transposed nuclei were partitioned into GEMs by using the Chromium Controller with Chip H for a target recovery of 5000 nuclei per sample. For samples used to assess potential artifacts due to FACS sorting, nuclei obtained from sorted and unsorted cells were loaded on separate channels of the same chip and parallelly processed for library preparation. After linear amplification, the resulting DNA was purified by sequential Dynabeads and SPRIselect reagent beads clean-ups. Libraries were indexed by PCR using the Single Index Kit N Set A (10X Genomics, PN-1000212) applying 10 cycles of amplification. Sequencing libraries were subjected to a final bead clean-up SPRIselect reagent and quantified on an Agilent Bioanalyzer High Sensitivity chip (Agilent Technologies). Finally, libraries were loaded on an Illumina NovaSeq 6000 with the following sequencing conditions: 50 bp (Read 1N) + 8 bp (i7 Index) + 16 bp (i5 Index) + 49 bp (Read 2N), aiming at a sequencing depth of >25,000 reads/nucleus. Single cell RNA and chromatin accessibility profiling Cryopreserved cells were thawed and cleaned from dead cells as described before (see scATAC-seq). Nuclei isolation was performed following the “Nuclei Isolation for Single Cell Multiome ATAC + Gene Expression Sequencing'' demonstrated protocol (10x Genomics; CG000365) starting from 0.5-1 M cells per sample and incubating on ice during 3 min for cell lysis. Nuclei were resuspended into the appropriate volume of chilled Diluted Nuclei Buffer (10X Genomics) to achieve a nuclei concentration of 925-2,300 nuclei/μl, suitable for a Target Nuclei Recovery of 7,000 per sample. The resulting nuclei concentration was determined by manual counting using a Neubauer chamber upon staining with Trypan Blue. GEX and ATAC-seq libraries were prepared following the Chromium Next GEM Single Cell Multiome ATAC + Gene Expression User Guide (10X Genomics; CG000338). Transposed nuclei were partitioned into GEMs by using the Chromium Controller with Chip J aiming at a target recovery of 7,000 nuclei per sample. After GEMs incubation for mRNA reverse transcription and transposed DNA barcoding, the resulting cDNA and barcoded gDNA were purified and pre-amplified during 7 cycles, following the 10X Genomics protocol. After a clean-up, 35 μl of the pre-amplified cDNA were amplified with 7 additional PCR cycles. The resulting cDNA was quantified on an Agilent Bioanalyzer High Sensitivity chip (Agilent Technologies) and 100 ng were used for library preparation. GEX libraries were indexed with 13 cycles of amplification using the Dual Index Plate TT Set A (10X Genomics; PN-3000431). In parallel, 40 μl of the pre-amplified DNA were indexed with 7 cycles of amplification using the Sample Index N Set A (10X Genomics; PN 3000427). Size distribution and concentration of full-length GEX and ATAC-seq libraries were verified on an Agilent Bioanalyzer High Sensitivity chip (Agilent Technologies). Finally, sequencing of GEX libraries was carried out on a NovaSeq 6000 sequencer (Illumina) using the following sequencing conditions: 28 bp (Read 1) + 8 bp (i7 index) + 0 bp (i5 index) + 89 bp (Read 2), to obtain approximately >20,000 paired-end reads per cell. ATAC-seq libraries were also sequenced with a NovaSeq 6000 sequencer (Illumina) using the following conditions: 50 bp (Read 1N) + 8 bp (i7 Index) + 16 bp (i5 Index) + 49 bp (Read 2N), aiming at a sequencing depth of >25,000 reads/nucleus. CITE-Seq Cryopreserved cells were thawed and FACS-sorted as previously described (see scATAC-seq and 3’ Single Cell RNA sequencing). For CITE-Seq experiments, samples were processed separately or processed in pools (subsequent demultiplexing by genotypes) before cell labeling with a custom panel of 192 oligo-barcoded antibodies (TotalSeq-C Custom Human Panel, Biolegend), following the same staining protocol of for Cell Hashing (see 3’ Single Cell RNA sequencing). Antibody details are included in Tables S23 and S24. Cells were loaded on the 10X Chromium Controller using the Next GEM Single Cell V(D)J Reagent Kits v1.1 with Feature Barcoding technology (10X Genomics, CG00208) according to manufacturer’s instructions. Each sample was loaded in duplicate for a total target recovery of 5,000 cells (20,000 for sample pools). After GEM dissolution and Dynabeads purification, 15 PCR cycles were done using the SC5ʹ Feature cDNA Primers (PN-1000080) to amplify the DNA from cell surface protein Feature Barcode oligos together with the full-length cDNA. The two products were separated by size selection and used for generating the different types of libraries. To construct the GEX library, the amplified full-length cDNA was fragmented, end repaired, A-tailed, and sample indexed using the Chromium Single Cell 5’ Library Construction Kit (10X Genomics, PN-1000020). For the V(D)J library, human T and B cell V(D)J sequences were enriched from the amplified cDNA with the Chromium Single Cell V(D)J Enrichment Kits (PN-1000005 and PN-1000016 for T and B cells respectively) followed by fragmentation, end repairing, A-tailing and sample indexing. Finally, the cell surface protein (CSP) library was generated from the amplified DNA from cell surface protein Feature Barcode by one-step PCR amplification using the Chromium Single Cell 5' Feature Barcode Library Kit (PN-1000080). Quantification and fragment size distribution of cDNAs and final libraries were determined using the Agilent 2100 BioAnalyzer High Sensitivity DNA kit (Agilent Technologies). All constructs were sequenced together on a Novaseq 6000 (Illumina), targeting a median sequencing depth of 20,000 (GEX), 2,000 (VDJ) and 8,000 (CSP) reads per cell. Spatial Transcriptomics (Visium OCT) Spatial visualization of gene expression within tonsil tissue was conducted using the Visium Spatial Gene Expression kit (10X Genomics) as per manufacturer's protocol. The OCT blocks were cut twice using a cryostat (Leica CM1950): a first time to assess RNA quality and assure a minimum RNA Integrity Number (RIN) number of 7 (RNA pico Chip) and a second time to mount a 10 μm section on the Visium slides. Slides were H&E stained before the sections were imaged using the NanoZoomer S60 (Hamamatsu) to assess tissue morphology and quality. The sections were then permeabilized for 6 min, according to the results of a corresponding Tissue Optimization experiment (10X Genomics, CG000238), and processed according to the Visium Spatial Gene Expression user guide (10X Genomics, CG000239). In short, tissue was lysed and reverse transcription was performed followed by second strand synthesis and cDNA denaturation. Spatially barcoded, full length cDNAs were amplified by PCR for 16 or 18 cycles, depending on the initial concentration previously determined by qPCR. Indexed sequencing libraries were generated via end repair, A-tailing, adaptor ligation and sample index PCR and analyzed using the Agilent 2100 BioAnalyzer. Libraries were sequenced on an Illumina NovaSeq 6000 with sequencing depth of ~100,000 reads per spot.

characteristic: description

text: adult

text: sleep apnea

characteristic: disease

characteristic: donorid

text: not collected

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: palatine tonsil

characteristic: organism part

text: 10x 3' v3

characteristic: protocol

text: Homo sapiens

text: male

characteristic: sex

text: scRNA Sample 15_hashed_hto

characteristic: title


E-MTAB-13687:scRNA Sample 16_hashed_hto

characteristic: ENA first public

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characteristic: External Id

text: CNAG-CRG

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-13687:scRNA Sample 16_hashed_hto

text: ArrayExpress

text: All

text: discovery

text: not collected

text: human

text: juvenile stage

characteristic: developmental stage

text: tonsillitis

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: palatine tonsil

text: 10x 3' v3

text: Homo sapiens

text: male

text: scRNA Sample 16_hashed_hto


E-MTAB-13687:scRNA Sample 16_not_hashed

text: CNAG-CRG

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-13687:scRNA Sample 16_not_hashed

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: All

text: discovery

characteristic: cohort

text: not collected

characteristic: collection date

text: human

characteristic: common name

text: juvenile stage

text: tonsillitis

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: palatine tonsil

text: 10x 3' v3

text: Homo sapiens

text: male

text: scRNA Sample 16_not_hashed


E-MTAB-13687:scRNA Sample 17_hashed_hto

text: CNAG-CRG

text: public

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text: ArrayExpress

text: All

text: discovery

text: not collected

text: human

characteristic: description

text: juvenile stage

text: tonsillitis

characteristic: disease

characteristic: donorid

text: not collected

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: palatine tonsil

characteristic: organism part

text: 10x 3' v3

characteristic: protocol

text: Homo sapiens

text: male

characteristic: sex

text: scRNA Sample 17_hashed_hto

characteristic: title


E-MTAB-13687:scRNA Sample 17_not_hashed

characteristic: ENA first public

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characteristic: External Id

text: CNAG-CRG

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-13687:scRNA Sample 17_not_hashed

text: ArrayExpress

text: All

text: discovery

text: not collected

text: human

text: juvenile stage

characteristic: developmental stage

text: tonsillitis

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: palatine tonsil

text: 10x 3' v3

text: Homo sapiens

text: male

text: scRNA Sample 17_not_hashed


E-MTAB-13687:scRNA Sample 2_hashed_hto

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characteristic: SRA accession

text: E-MTAB-13687:scRNA Sample 2_hashed_hto

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text: ArrayExpress

characteristic: broker name

text: All

text: discovery

characteristic: cohort

text: not collected

characteristic: collection date

text: human

characteristic: common name

text: juvenile stage

text: tonsillitis

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: palatine tonsil

text: 10x 3' v3

text: Homo sapiens

text: female

text: scRNA Sample 2_hashed_hto


E-MTAB-13687:scRNA Sample 2_not_hashed

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text: All

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characteristic: description

text: juvenile stage

text: tonsillitis

characteristic: disease

characteristic: donorid

text: not collected

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: palatine tonsil

characteristic: organism part

text: 10x 3' v3

characteristic: protocol

text: Homo sapiens

text: female

characteristic: sex

text: scRNA Sample 2_not_hashed

characteristic: title


E-MTAB-13687:scRNA Sample 3_hashed_hto

characteristic: ENA first public

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text: CNAG-CRG

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text: public

text: E-MTAB-13687:scRNA Sample 3_hashed_hto

text: ArrayExpress

text: All

text: discovery

text: not collected

text: human

text: juvenile stage

characteristic: developmental stage

text: tonsillitis

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: palatine tonsil

text: 10x 3' v3

text: Homo sapiens

text: female

text: scRNA Sample 3_hashed_hto


E-MTAB-13687:scRNA Sample 3_not_hashed

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text: not collected

characteristic: collection date

text: human

characteristic: common name

text: juvenile stage

text: tonsillitis

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: palatine tonsil

text: 10x 3' v3

text: Homo sapiens

text: female

text: scRNA Sample 3_not_hashed


E-MTAB-13687:scRNA Sample 4_hashed_hto

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text: ArrayExpress

text: All

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text: not collected

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characteristic: description

text: juvenile stage

text: tonsillitis

characteristic: disease

characteristic: donorid

text: not collected

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characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: palatine tonsil

characteristic: organism part

text: 10x 3' v3

characteristic: protocol

text: Homo sapiens

text: female

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text: scRNA Sample 4_hashed_hto

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E-MTAB-13687:scRNA Sample 4_not_hashed

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text: CNAG-CRG

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text: ArrayExpress

text: All

text: discovery

text: not collected

text: human

text: juvenile stage

characteristic: developmental stage

text: tonsillitis

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: palatine tonsil

text: 10x 3' v3

text: Homo sapiens

text: female

text: scRNA Sample 4_not_hashed


E-MTAB-13687:scRNA Sample 5_hashed_hto

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text: E-MTAB-13687:scRNA Sample 5_hashed_hto

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text: ArrayExpress

characteristic: broker name

text: All

text: discovery

characteristic: cohort

text: not collected

characteristic: collection date

text: human

characteristic: common name

text: adult

text: sleep apnea

text: not collected

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text: Homo sapiens

text: Homo sapiens

text: palatine tonsil

text: 10x 3' v3

text: Homo sapiens

text: male

text: scRNA Sample 5_hashed_hto


E-MTAB-13687:scRNA Sample 5_not_hashed

text: CNAG-CRG

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text: E-MTAB-13687:scRNA Sample 5_not_hashed

text: ArrayExpress

text: All

text: discovery

text: not collected

text: human

characteristic: description

text: adult

text: sleep apnea

characteristic: disease

characteristic: donorid

text: not collected

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: palatine tonsil

characteristic: organism part

text: 10x 3' v3

characteristic: protocol

text: Homo sapiens

text: male

characteristic: sex

text: scRNA Sample 5_not_hashed

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E-MTAB-13687:scRNA Sample 6_hashed_hto

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text: CNAG-CRG

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text: E-MTAB-13687:scRNA Sample 6_hashed_hto

text: ArrayExpress

text: All

text: discovery

text: not collected

text: human

text: adult

characteristic: developmental stage

text: sleep apnea

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: palatine tonsil

text: 10x 3' v3

text: Homo sapiens

text: male

text: scRNA Sample 6_hashed_hto


E-MTAB-13687:scRNA Sample 7_hashed_hto

text: CNAG-CRG

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characteristic: SRA accession

text: E-MTAB-13687:scRNA Sample 7_hashed_hto

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: All

text: discovery

characteristic: cohort

text: not collected

characteristic: collection date

text: human

characteristic: common name

text: adult

text: surgery benign pharyngeal squamous papillomatosis

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: palatine tonsil

text: 10x 3' v3

text: Homo sapiens

text: male

text: scRNA Sample 7_hashed_hto


E-MTAB-13687:scRNA Sample 7_not_hashed

text: CNAG-CRG

text: public

text: E-MTAB-13687:scRNA Sample 7_not_hashed

text: ArrayExpress

text: All

text: discovery

text: not collected

text: human

characteristic: description

text: adult

characteristic: disease

characteristic: donorid

text: not collected

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: palatine tonsil

characteristic: organism part

text: 10x 3' v3

characteristic: protocol

text: Homo sapiens

text: male

characteristic: sex

text: scRNA Sample 7_not_hashed

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: External Id

text: Candiolo Cancer Institute

characteristic: INSDC center name

characteristic: INSDC last update

text: public

tag: Namespace:Candiolo Cancer Institute

text: human

text: Italy

characteristic: geographic location (country and/or sea)

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Cell line


text: Institute of Cellular Biology and Pathology \"N. Simionescu\"

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: primary cell line

text: endothelial cell

text: not collected

characteristic: collection date

text: human

text: Protocols: VECs after interaction with monocytes in NG or HG conditions, monocytes were removed by washing with PBS and total RNA from VECs was isolated with TRIzol reagent and Phasemaker tubes. VECs in NG or HG media were used as controls. For each condition, 3 biological replicates were collected in 3 independent experiments.  Primary human valvular endothelial cells (VEC) were sourced from aortic valve leaflets extracted from patients undergoing aortic valve replacement surgery. VEC population was subsequently cultured in EGM-2 media supplemented with 10% FBS and 1% penicillin/streptomycin in normal glucose concentration (NG – 5 mM glucose) or in high glucose concentrations (HG – 33 mM glucose).  Monocyte‐like cell line THP‐1 was grown in suspension in the RPMI 1640 culture medium containing 10% FCS and were split 1:5, twice a week.  To set up the VEC-monocyte co-culture, we first incubated the monocytes for 72 hours in either normal glucose (NG) media (Mo-NG) or high glucose (HG) media (Mo-HG). Then, we introduced 1.5x10^6 monocytes/ml to the VEC monolayer for 2 hours. For this addition, Mo-NG was added to VECs in NG media, and Mo-HG was added to VECs in HG media, matching each monocyte type with its respective media. As controls, we used VECs in NG and VECs in HG media without monocytes. Total RNA was extracted using the manufacturer's recommended protocol: The Phasemaker tubes were centrifuged for 30 seconds at 12,000-16,000 × g before use. Samples were lysed and homogenized in TRIzol Reagent. After adding chloroform, the mixture was centrifuged to separate into three layers: a clear upper aqueous layer containing RNA, an interphase, and a red lower organic layer with DNA and proteins. The Phasemaker tubes facilitated the transfer of the aqueous layer by creating a barrier between the upper and lower phases. RNA was then precipitated from this aqueous layer using isopropanol. Following precipitation, the RNA was washed to remove any impurities and subsequently resuspended for downstream applications. RNA concentration and purity were quantified using the NanoDrop 1000 (ThermoFisher, USA).  Library construction, quality control and sequencing were performed by Novogene (Cambridge, UK). A total amount of 1 µg RNA per sample was used as input for RNA analysis.  Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dUTP. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection.

text: adult

characteristic: developmental stage

text: aortic valve disease

text: wild type genotype

characteristic: genotype

text: not collected

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: aortic valve

characteristic: replicate

text: Homo sapiens

text: NG - Normal Glucose

characteristic: stimulus


text: public

text: ArrayExpress

text: primary cell line

text: endothelial cell

text: not collected

text: human

characteristic: common name

characteristic: description

text: adult

text: aortic valve disease

characteristic: disease

text: wild type genotype

text: not collected

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: aortic valve

characteristic: organism part

text: Homo sapiens

text: HG - high glucose

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: INSDC center name

text: public

text: ArrayExpress

text: primary cell line

text: endothelial cell

text: not collected

text: human

text: adult

text: aortic valve disease

text: wild type genotype

text: not collected

characteristic: geographic location (country and/or sea)

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: aortic valve

text: Homo sapiens

text: Interaction with monocytes in NG


text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: primary cell line

text: endothelial cell

text: not collected

text: human

text: adult

text: aortic valve disease

text: wild type genotype

text: not collected

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: aortic valve

text: Homo sapiens

text: Interaction with monocytes in HG


text: IAI - Institut des Agents Infectieux

text: public

characteristic: collection date

text: S. haemolyticus strain isolated from blood

text: France

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

text: blood

text: Staphylococcus haemolyticus

text: Staphylococcus haemolyticus

characteristic: strain


text: IAI - Institut des Agents Infectieux

text: public

text: S. haemolyticus strain isolated from carriage/skin

characteristic: description

text: France

text: not provided

text: Homo sapiens

text: not provided

text: carriage/skin

text: Staphylococcus haemolyticus

text: Staphylococcus haemolyticus


text: IAI - Institut des Agents Infectieux

text: public

text: S. haemolyticus strain isolated from urogenital

text: France

text: not provided

text: Homo sapiens

text: not provided

text: urogenital

text: Staphylococcus haemolyticus

text: Staphylococcus haemolyticus


text: IAI - Institut des Agents Infectieux

text: public

text: S. haemolyticus strain isolated from other

text: France

text: not provided

text: Homo sapiens

text: not provided

text: other

text: Staphylococcus haemolyticus

text: Staphylococcus haemolyticus


text: IAI - Institut des Agents Infectieux

text: public

text: S. haemolyticus strain isolated from material (non environment)

text: France

text: not provided

text: Homo sapiens

text: not provided

text: material (non environment)

text: Staphylococcus haemolyticus

text: Staphylococcus haemolyticus


text: IAI - Institut des Agents Infectieux

text: public

text: S. haemolyticus strain isolated from bone joint infection

text: France

text: not provided

text: Homo sapiens

text: not provided

text: bone joint infection

text: Staphylococcus haemolyticus

text: Staphylococcus haemolyticus


text: IAI - Institut des Agents Infectieux

text: public

text: S. haemolyticus strain isolated from abces

text: France

text: not provided

text: Homo sapiens

text: not provided

text: abces

text: Staphylococcus haemolyticus

text: Staphylococcus haemolyticus


text: IAI - Institut des Agents Infectieux

text: public

text: S. haemolyticus strain isolated from respiratory

text: France

text: not provided

text: Homo sapiens

text: not provided

text: respiratory

text: Staphylococcus haemolyticus

text: Staphylococcus haemolyticus


text: IAI - Institut des Agents Infectieux

text: public

text: S. haemolyticus strain isolated from environment

text: France

text: not provided

text: Homo sapiens

text: not provided

text: environment

text: Staphylococcus haemolyticus

text: Staphylococcus haemolyticus


text: IAI - Institut des Agents Infectieux

text: public

text: S. haemolyticus strain isolated from Catheter

text: France

text: not provided

text: Homo sapiens

text: not provided

text: Catheter

text: Staphylococcus haemolyticus

text: Staphylococcus haemolyticus


text: IAI - Institut des Agents Infectieux

text: public

text: S. haemolyticus strain isolated from eye

text: France

text: not provided

text: Homo sapiens

text: not provided

text: eye

text: Staphylococcus haemolyticus

text: Staphylococcus haemolyticus


text: IAI - Institut des Agents Infectieux

text: public

text: S. haemolyticus strain isolated from not known

text: France

text: not provided

text: Homo sapiens

text: not provided

text: not known

text: Staphylococcus haemolyticus

text: Staphylococcus haemolyticus


text: IAI - Institut des Agents Infectieux

text: public

text: S. haemolyticus strain isolated from ORL

text: France

text: not provided

text: Homo sapiens

text: not provided

text: ORL

text: Staphylococcus haemolyticus

text: Staphylococcus haemolyticus


text: University of Duisburg-Essen, Aquatic Ecosystem Research

text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 1


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 2


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 3


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 4


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 5


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 6


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 7


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 8


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 9


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 10


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 11


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 12


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 14


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 15


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 17


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 18


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 19


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 20


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 21


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 22


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 23


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 24


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 25


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 26


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 27


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 28


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 29


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 30


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 31


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 32


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 33


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 35


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 37


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 38


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 40


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 41


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 42


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 43


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 44


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 45


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 46


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 47


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 48


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 49


GmO

text: public

text: GmO

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 50


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 51


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 52


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 53


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 54


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 55


GmB

text: public

text: GmB

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 56


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 57


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 58


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 59


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 60


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 61


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 62


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 63


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 65


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 66


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 67


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 69


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 70


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 71


UB

text: public

text: UB

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 72


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 73


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 74


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 75


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 76


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 78


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 79


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 80


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 81


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 82


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 83


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 85


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 86


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 87


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 88


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 89


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 90


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 91


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 92


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 93


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 94


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 95


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 96


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 97


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 99


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 100


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 101


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 102


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 1


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 2


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 3


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 4


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 5


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 6


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 7


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 8


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 9


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 10


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 11


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 12


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 14


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 15


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 17


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 18


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 19


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 20


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 21


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 22


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 23


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 24


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 25


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 26


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 27


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 28


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 29


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 30


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 31


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 32


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 33


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 35


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 37


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 38


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 40


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 41


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 42


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 43


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 44


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 45


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 46


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 47


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 48


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 49


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 50


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 51


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 52


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 53


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 54


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 55


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 56


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 57


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 58


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 59


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 60


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 61


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 62


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 63


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 65


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 66


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 67


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 69


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 70


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 71


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 72


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 73


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 74


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 75


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 76


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 78


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 79


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 80


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 81


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 82


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 83


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 85


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 86


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 87


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 88


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 89


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 90


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 91


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 92


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 93


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 94


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 95


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 96


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 97


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 99


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 100


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 101


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 102


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 1


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 3


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 5


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 7


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 9


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 11


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 13


text: public

text: eDNA sample

text: Germany

text: freshwater metagenome

text: freshwater metagenome

text: EPTDr2n sample 15


text: Candiolo Cancer Institute

text: public

text: human

characteristic: common name

text: B1003_XL5 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: B1003_XLSP CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: C146 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: C170 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: CL34 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: CL40 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: CO115 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: COGA10 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: COGA12 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: COGA3 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: COLO205 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: COLO320 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: COLO320DM CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: COLO320HSR CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

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text: COLO60H CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: CRC0322_XL CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: CRC1360_XL CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: CX1 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


GEO

text: Candiolo Cancer Institute

text: public

text: GEO

text: human

text: GEO CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens

text: GEO


text: Candiolo Cancer Institute

text: public

text: human

text: GP2D CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: GP5D CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: HCA7 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: HCT116 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: HCT8 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: HDC135 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: HDC143 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: HDC54 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: HDC8 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: HDC9 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: HHC6548 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: HROBMCO1 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: HROC107_cT0M2 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: HROC113 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: HROC126 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: HROC147_MET CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: HROC173 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: HROC212 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: HROC222_T1M2 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: HROC257_T0M1 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: HROC277_MET2 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: HROC57 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: HUTU80 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: IRCC10_A_HL CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: IRCC3_HL CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

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text: Italy

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text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: JVE187 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: JVE192 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: JVE241 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: KM12SM CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: LM0201_A_XL CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

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text: Italy

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text: Homo sapiens


text: Candiolo Cancer Institute

text: public

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text: Italy

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text: Homo sapiens


text: Candiolo Cancer Institute

text: public

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text: Italy

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text: Candiolo Cancer Institute

text: public

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text: Italy

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text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

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text: Italy

text: Homo sapiens

text: Homo sapiens


RKO

text: Candiolo Cancer Institute

text: public

text: RKO

text: human

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text: Italy

text: Homo sapiens

text: Homo sapiens

text: RKO


text: Candiolo Cancer Institute

text: public

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

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text: Italy

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text: Homo sapiens


text: Candiolo Cancer Institute

text: public

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text: Italy

text: Homo sapiens

text: Homo sapiens


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text: public

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

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text: Italy

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text: Homo sapiens


text: Candiolo Cancer Institute

text: public

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

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text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: SW48 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: SW620 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: V457 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: V703 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: V9P CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: VACO400 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: VACO432 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: VACO5 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Candiolo Cancer Institute

text: public

text: human

text: VACO6 CRC cell line

text: Italy

text: Homo sapiens

text: Homo sapiens


text: Statens Serum Institut

text: public

text: building

characteristic: broad-scale environmental context

unit: m

characteristic: depth

text: wastewater

characteristic: environmental medium

text: Denmark

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: wastewater treatment plant

characteristic: local environmental context

text: Severe acute respiratory syndrome coronavirus 2

text: SARS-CoV-2 wastewater Nanopore sequencing, Denmark

characteristic: project name


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Statens Serum Institut

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: building

text: wastewater

text: Denmark

characteristic: geographic location (country and/or sea)

text: wastewater treatment plant

text: Gammel_Køge_Landevej_(Avedøre)_2021-11-23

characteristic: title


text: Statens Serum Institut

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: building

characteristic: collection date

text: wastewater

text: Denmark

text: wastewater treatment plant

characteristic: organism

text: Gammel_Køge_Landevej_(Avedøre)_2021-12-07


text: Statens Serum Institut

text: public

text: building

characteristic: broad-scale environmental context

text: wastewater

text: Denmark

text: wastewater treatment plant

text: Gammel_Køge_Landevej_(Avedøre)_2021-12-14


text: Statens Serum Institut

text: public

text: building

characteristic: depth

text: wastewater

text: Denmark

text: wastewater treatment plant

text: Gammel_Køge_Landevej_(Avedøre)_2021-12-28


text: Statens Serum Institut

text: public

text: building

text: wastewater

characteristic: environmental medium

text: Denmark

text: wastewater treatment plant

text: Gammel_Køge_Landevej_(Avedøre)_2022-01-04


text: Statens Serum Institut

text: public

text: building

text: wastewater

text: Denmark

characteristic: geographic location (latitude)

text: wastewater treatment plant

text: Gammel_Køge_Landevej_(Avedøre)_2022-01-18


text: Statens Serum Institut

text: public

text: building

text: wastewater

text: Denmark

characteristic: geographic location (longitude)

text: wastewater treatment plant

text: Gammel_Køge_Landevej_(Avedøre)_2022-01-25


text: Statens Serum Institut

text: public

text: building

text: wastewater

text: Denmark

text: wastewater treatment plant

characteristic: local environmental context

text: Gammel_Køge_Landevej_(Avedøre)_2022-02-15


text: Statens Serum Institut

text: public

text: building

text: wastewater

text: Denmark

text: wastewater treatment plant

characteristic: project name

text: Gammel_Køge_Landevej_(Avedøre)_2022-03-01


characteristic: ENA first public

text: Statens Serum Institut

text: public

text: building

text: wastewater

text: Denmark

text: wastewater treatment plant

text: Gammel_Køge_Landevej_(Avedøre)_2022-03-15


characteristic: ENA-CHECKLIST

text: Statens Serum Institut

text: public

text: building

text: wastewater

text: Denmark

text: wastewater treatment plant

text: Gammel_Køge_Landevej_(Avedøre)_2022-03-22


text: Statens Serum Institut

characteristic: INSDC center name

text: public

text: building

text: wastewater

text: Denmark

text: wastewater treatment plant

text: Gammel_Køge_Landevej_(Avedøre)_2022-05-24


text: UNIVERSITE DE PARIS

text: public

characteristic: INSDC status

text: human

characteristic: common name

text: Late Neolithic

characteristic: description

text: France

text: Grand Est, Bréviandes

characteristic: geographic location (region and locality)

text: petrous bone

text: Homo sapiens

text: Homo sapiens

text: F

characteristic: sex


text: UNIVERSITE DE PARIS

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: human

text: Late Neolithic

text: France

characteristic: geographic location (country and/or sea)

text: Grand Est, Bréviandes

text: petrous bone

text: Homo sapiens

text: Homo sapiens

text: M

characteristic: title


text: UNIVERSITE DE PARIS

text: public

text: human

text: Bell Beaker

text: France

text: Ile-de-France, Saint-Martin-la-Garenne

text: petrous bone

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: M


text: UO IE2 Phillips lab

text: public

text: ena-SAMPLE-TAB-23-03-2024-00:35:22:956-118303

text: The inbred strain CFB2252 was initially isolated as a single female from a decaying marigold flower in India. It underwent twenty sib-matings, with expansion every five generations. Then, for twenty-two generations, a single gravid female was selected for propagation. The population was subsequently expanded for eight generations. Finally, the strain underwent forty generations of single-female propagation.

text: India

text: Shyamnagar

text: Rotten marigold flower

text: Asher D. Cutter at the University of Toronto; Charles F. Baer at the Florida State University; Patrick C. Phillips at the University of Oregon

text: Caenorhabditis brenneri

text: Caenorhabditis brenneri

characteristic: strain

text: Caenorhabditis brenneri, strain CFB2252


text: UNIVERSITY OF WESTERN ONTARIO

text: public

text: sub-boreal

characteristic: broad-scale environmental context

characteristic: depth

text: Tailings Soil

characteristic: elevation

text: Waste material

characteristic: environmental medium

text: Canada

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Mine tailings

characteristic: local environmental context

text: uncultured bacterium

text: organic covers response to climate change

text: uncultured bacterium


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Finnish Food Authority

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: L. monocytogenes food sample

characteristic: description

text: Finland

characteristic: isolate

text: fava bean product

text: Listeria monocytogenes

text: Listeria monocytogenes

text: L. monocytogenes FIXT-750


text: Finnish Food Authority

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: L. monocytogenes food sample

text: Finland

characteristic: geographic location (country and/or sea)

text: fava bean product

text: Listeria monocytogenes

text: Listeria monocytogenes

text: L. monocytogenes FIXT-991


text: Finnish Food Authority

text: public

text: L. monocytogenes food sample

text: Finland

text: fava bean product

text: Listeria monocytogenes

characteristic: organism

text: Listeria monocytogenes

text: L. monocytogenes FIXT-992

characteristic: title