text: barrnap (version 0.9)

characteristic: 16S recovery software

text: No

characteristic: 16s recovered

text: no

characteristic: 23s recovered

text: no

characteristic: 5s recovered

text: CAU

text: public

characteristic: MAG coverage software

characteristic: SRA accession

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:828-115443

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

characteristic: binning parameters

characteristic: binning software

text: host associated gut microbiome

characteristic: completeness score

text: CheckM (version 1.1.2)

characteristic: completeness software

characteristic: contamination score

text: MAG_BA_Sporofaciens_01: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

text: host associated gut microbiome

text: Germany

text: mouse colon contents

text: host associated gut microbiome

text: mouse gut metagenome

characteristic: metagenomic source

characteristic: number of standard tRNAs extracted

text: uncultured Lachnospiraceae bacterium

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

characteristic: tRNA extraction software

text: GTDB-Tk, r214

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker


status: PUBLIC

text: barrnap (version 0.9)

tag: attribute

characteristic: 16S recovery software

text: No

characteristic: 16s recovered

text: yes

characteristic: 23s recovered

text: no

characteristic: 5s recovered

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: GC content

text: CAU

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: Bowtie (version 2.2.5) with jgi_summarize_bam_contig_depths script from MetaBAT (version 2.12.1)

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:828-115444

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes hybrid (SPAdes version 3.13.1)

text: coverage depth and k-mer binning. Scaffolds were filtered for min. length 1000bp and min. coverage 7.7815. Quality-controlled metagenomic reads were mapped back to the filtered scaffolds with Bowtie (version 2.2.5); the insert size was 0–1000 bp in very sensitive, non-deterministic, “fr” stranded mode with end-to-end alignment. Non-unique mappings and unaligned reads were discarded. The scaffold coverage depth was determined with the jgi_summarize_bam_contig_depths script from MetaBAT (version 2.12.1). Scaffolds were broken into 10 kbp chunks for CONCOCT.

text: MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), MaxBin (version 2.2.4), DASTool (version 1.1.2)

text: host associated gut microbiome

characteristic: broad-scale environmental context

characteristic: collection date

text: CheckM (version 1.1.2)

text: MAG_BA_Sporofaciens_02: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

characteristic: description

text: host associated gut microbiome

characteristic: environmental medium

text: Germany

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mouse colon contents

text: host associated gut microbiome

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text: Baseline aging (BA); Aging-associated loss of metabolic host-microbiome-interactions in mice

characteristic: project name

text: ERS18444878,ERS18444879,ERS18444880,ERS18444881,ERS18444882,ERS18444883,ERS18444884,ERS18444885,ERS18444886,ERS18444887,ERS18444888,ERS18444889,ERS18444890,ERS18444891,ERS18444892,ERS18444893,ERS18444894,ERS18444895,ERS18444896,ERS18444897,ERS18444898,ERS18444899,ERS18444900,ERS18444901,ERS18444902,ERS18444903,ERS18444904,ERS18444905,ERS18444906,ERS18444907,ERS18444908,ERS18444909,ERS18444910,ERS18444911,ERS18444912,ERS18444913,ERS18444914,ERS18444915,ERS18444916,ERS18444917,ERS18444918,ERS18444919,ERS18444920,ERS18444921,ERS18444922,ERS18444923,ERS18444924,ERS18444925,ERS18444926,ERS18444927,ERS18444928,ERS18444929,ERS18590020,ERS18590021,ERS18590022,ERS18590023,ERS18590024,ERS18590025,ERS18590026,ERS18590027

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

text: GTDB-Tk, r214

text: multi-marker approach

characteristic: title


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: No

characteristic: 16s recovered

text: no

characteristic: 23s recovered

text: no

characteristic: 5s recovered

text: CAU

text: public

characteristic: MAG coverage software

characteristic: SRA accession

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:828-115445

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

characteristic: binning parameters

characteristic: binning software

text: host associated gut microbiome

characteristic: completeness score

text: CheckM (version 1.1.2)

characteristic: completeness software

characteristic: contamination score

text: MAG_BA_Lachnospiraceae_03: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

text: host associated gut microbiome

text: Germany

text: mouse colon contents

text: host associated gut microbiome

text: mouse gut metagenome

characteristic: metagenomic source

characteristic: number of standard tRNAs extracted

text: uncultured Lachnospiraceae bacterium

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

characteristic: tRNA extraction software

text: GTDB-Tk, r214

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: No

characteristic: 16s recovered

text: no

characteristic: 23s recovered

text: no

characteristic: 5s recovered

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: GC content

text: CAU

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:829-115446

text: host associated gut microbiome

characteristic: broad-scale environmental context

characteristic: collection date

text: CheckM (version 1.1.2)

text: MAG_BA_Choladocola_01: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

characteristic: description

text: host associated gut microbiome

characteristic: environmental medium

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mouse colon contents

text: host associated gut microbiome

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

characteristic: project name

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

text: GTDB-Tk, r214

text: multi-marker approach

characteristic: title


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: No

characteristic: 16s recovered

text: no

characteristic: 23s recovered

text: no

characteristic: 5s recovered

text: CAU

text: public

characteristic: MAG coverage software

characteristic: SRA accession

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:829-115447

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

characteristic: binning parameters

characteristic: binning software

text: host associated gut microbiome

characteristic: completeness score

text: CheckM (version 1.1.2)

characteristic: completeness software

characteristic: contamination score

text: MAG_BA_Choladocola_02: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

text: host associated gut microbiome

text: Germany

text: mouse colon contents

text: host associated gut microbiome

text: mouse gut metagenome

characteristic: metagenomic source

characteristic: number of standard tRNAs extracted

text: uncultured Lachnospiraceae bacterium

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

characteristic: tRNA extraction software

text: GTDB-Tk, r214

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: Yes

characteristic: 16s recovered

text: yes

characteristic: 23s recovered

text: no

characteristic: 5s recovered

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: GC content

text: CAU

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:829-115448

text: host associated gut microbiome

characteristic: broad-scale environmental context

characteristic: collection date

text: CheckM (version 1.1.2)

text: MAG_BA_Merdisoma_01: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

characteristic: description

text: host associated gut microbiome

characteristic: environmental medium

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mouse colon contents

text: host associated gut microbiome

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

characteristic: project name

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

text: GTDB-Tk, r214

text: multi-marker approach

characteristic: title


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: No

characteristic: 16s recovered

text: no

characteristic: 23s recovered

text: no

characteristic: 5s recovered

text: CAU

text: public

text: Bowtie (version 2.2.5) with jgi_summarize_bam_contig_depths script from MetaBAT (version 2.12.1)

characteristic: MAG coverage software

characteristic: SRA accession

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:829-115449

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

characteristic: binning parameters

characteristic: binning software

text: host associated gut microbiome

characteristic: completeness score

text: CheckM (version 1.1.2)

characteristic: completeness software

characteristic: contamination score

text: MAG_BA_Lachnospiraceae_04: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

text: host associated gut microbiome

text: Germany

text: mouse colon contents

text: host associated gut microbiome

text: mouse gut metagenome

characteristic: metagenomic source

characteristic: number of standard tRNAs extracted

text: uncultured Lachnospiraceae bacterium

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

characteristic: tRNA extraction software

text: GTDB-Tk, r214

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: No

characteristic: 16s recovered

text: no

characteristic: 23s recovered

text: no

characteristic: 5s recovered

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: GC content

text: CAU

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:830-115450

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes hybrid (SPAdes version 3.13.1)

text: host associated gut microbiome

characteristic: broad-scale environmental context

characteristic: collection date

text: CheckM (version 1.1.2)

text: MAG_BA_Lachnospiraceae_05: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

characteristic: description

text: host associated gut microbiome

characteristic: environmental medium

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mouse colon contents

text: host associated gut microbiome

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

characteristic: project name

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

text: GTDB-Tk, r214

text: multi-marker approach

characteristic: title


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: No

characteristic: 16s recovered

text: yes

characteristic: 23s recovered

text: no

characteristic: 5s recovered

text: CAU

text: public

characteristic: MAG coverage software

characteristic: SRA accession

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:830-115451

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: coverage depth and k-mer binning. Scaffolds were filtered for min. length 1000bp and min. coverage 7.7815. Quality-controlled metagenomic reads were mapped back to the filtered scaffolds with Bowtie (version 2.2.5); the insert size was 0–1000 bp in very sensitive, non-deterministic, “fr” stranded mode with end-to-end alignment. Non-unique mappings and unaligned reads were discarded. The scaffold coverage depth was determined with the jgi_summarize_bam_contig_depths script from MetaBAT (version 2.12.1). Scaffolds were broken into 10 kbp chunks for CONCOCT.

characteristic: binning parameters

text: MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), MaxBin (version 2.2.4), DASTool (version 1.1.2)

characteristic: binning software

text: host associated gut microbiome

characteristic: completeness score

text: CheckM (version 1.1.2)

characteristic: completeness software

characteristic: contamination score

text: MAG_BA_Lachnospiraceae_06: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

text: host associated gut microbiome

text: Germany

text: mouse colon contents

text: host associated gut microbiome

text: mouse gut metagenome

characteristic: metagenomic source

characteristic: number of standard tRNAs extracted

text: uncultured Lachnospiraceae bacterium

text: Baseline aging (BA); Aging-associated loss of metabolic host-microbiome-interactions in mice

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

characteristic: tRNA extraction software

text: GTDB-Tk, r214

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: Yes

characteristic: 16s recovered

text: no

characteristic: 23s recovered

text: no

characteristic: 5s recovered

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: GC content

text: CAU

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:830-115452

text: host associated gut microbiome

characteristic: broad-scale environmental context

characteristic: collection date

text: CheckM (version 1.1.2)

text: MAG_BA_Lachnospiraceae_07: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

characteristic: description

text: host associated gut microbiome

characteristic: environmental medium

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mouse colon contents

text: host associated gut microbiome

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

characteristic: project name

text: ERS18444878,ERS18444879,ERS18444880,ERS18444881,ERS18444882,ERS18444883,ERS18444884,ERS18444885,ERS18444886,ERS18444887,ERS18444888,ERS18444889,ERS18444890,ERS18444891,ERS18444892,ERS18444893,ERS18444894,ERS18444895,ERS18444896,ERS18444897,ERS18444898,ERS18444899,ERS18444900,ERS18444901,ERS18444902,ERS18444903,ERS18444904,ERS18444905,ERS18444906,ERS18444907,ERS18444908,ERS18444909,ERS18444910,ERS18444911,ERS18444912,ERS18444913,ERS18444914,ERS18444915,ERS18444916,ERS18444917,ERS18444918,ERS18444919,ERS18444920,ERS18444921,ERS18444922,ERS18444923,ERS18444924,ERS18444925,ERS18444926,ERS18444927,ERS18444928,ERS18444929,ERS18590020,ERS18590021,ERS18590022,ERS18590023,ERS18590024,ERS18590025,ERS18590026,ERS18590027

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

text: GTDB-Tk, r214

text: multi-marker approach

characteristic: title


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: No

characteristic: 16s recovered

text: no

characteristic: 23s recovered

text: no

characteristic: 5s recovered

text: CAU

text: public

characteristic: MAG coverage software

characteristic: SRA accession

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:830-115453

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

characteristic: binning parameters

characteristic: binning software

text: host associated gut microbiome

characteristic: completeness score

text: CheckM (version 1.1.2)

characteristic: completeness software

characteristic: contamination score

text: MAG_BA_Lachnospiraceae_08: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

text: host associated gut microbiome

text: Germany

text: mouse colon contents

text: host associated gut microbiome

text: mouse gut metagenome

characteristic: metagenomic source

characteristic: number of standard tRNAs extracted

text: uncultured Lachnospiraceae bacterium

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

characteristic: tRNA extraction software

text: GTDB-Tk, r214

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: No

characteristic: 16s recovered

text: no

characteristic: 23s recovered

text: no

characteristic: 5s recovered

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: GC content

text: CAU

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: Bowtie (version 2.2.5) with jgi_summarize_bam_contig_depths script from MetaBAT (version 2.12.1)

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:830-115454

text: host associated gut microbiome

characteristic: broad-scale environmental context

characteristic: collection date

text: CheckM (version 1.1.2)

text: MAG_BA_Lachnospiraceae_09: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

characteristic: description

text: host associated gut microbiome

characteristic: environmental medium

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mouse colon contents

text: host associated gut microbiome

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

characteristic: project name

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

text: GTDB-Tk, r214

text: multi-marker approach

characteristic: title


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: Yes

characteristic: 16s recovered

text: no

characteristic: 23s recovered

text: no

characteristic: 5s recovered

text: CAU

text: public

characteristic: MAG coverage software

characteristic: SRA accession

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:831-115455

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

characteristic: binning parameters

characteristic: binning software

text: host associated gut microbiome

characteristic: completeness score

text: CheckM (version 1.1.2)

characteristic: completeness software

characteristic: contamination score

text: MAG_BA_Lachnospiraceae_10: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

text: host associated gut microbiome

text: Germany

text: mouse colon contents

text: host associated gut microbiome

text: mouse gut metagenome

characteristic: metagenomic source

characteristic: number of standard tRNAs extracted

text: uncultured Lachnospiraceae bacterium

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

characteristic: tRNA extraction software

text: GTDB-Tk, r214

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: No

characteristic: 16s recovered

text: no

characteristic: 23s recovered

text: no

characteristic: 5s recovered

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: GC content

text: CAU

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:831-115456

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes hybrid (SPAdes version 3.13.1)

text: host associated gut microbiome

characteristic: broad-scale environmental context

characteristic: collection date

text: CheckM (version 1.1.2)

text: MAG_BA_Lachnospiraceae_11: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

characteristic: description

text: host associated gut microbiome

characteristic: environmental medium

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mouse colon contents

text: host associated gut microbiome

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

characteristic: project name

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

text: GTDB-Tk, r214

text: multi-marker approach

characteristic: title


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: Yes

characteristic: 16s recovered

text: no

characteristic: 23s recovered

text: no

characteristic: 5s recovered

text: CAU

text: public

characteristic: MAG coverage software

characteristic: SRA accession

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:831-115457

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: coverage depth and k-mer binning. Scaffolds were filtered for min. length 1000bp and min. coverage 7.7815. Quality-controlled metagenomic reads were mapped back to the filtered scaffolds with Bowtie (version 2.2.5); the insert size was 0–1000 bp in very sensitive, non-deterministic, “fr” stranded mode with end-to-end alignment. Non-unique mappings and unaligned reads were discarded. The scaffold coverage depth was determined with the jgi_summarize_bam_contig_depths script from MetaBAT (version 2.12.1). Scaffolds were broken into 10 kbp chunks for CONCOCT.

characteristic: binning parameters

text: MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), MaxBin (version 2.2.4), DASTool (version 1.1.2)

characteristic: binning software

text: host associated gut microbiome

characteristic: completeness score

text: CheckM (version 1.1.2)

characteristic: completeness software

characteristic: contamination score

text: MAG_BA_Lachnospiraceae_12: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

text: host associated gut microbiome

text: Germany

text: mouse colon contents

text: host associated gut microbiome

text: mouse gut metagenome

characteristic: metagenomic source

characteristic: number of standard tRNAs extracted

text: uncultured Lachnospiraceae bacterium

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

characteristic: tRNA extraction software

text: GTDB-Tk, r214

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: Yes

characteristic: 16s recovered

text: no

characteristic: 23s recovered

text: no

characteristic: 5s recovered

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: GC content

text: CAU

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:831-115458

text: host associated gut microbiome

characteristic: broad-scale environmental context

characteristic: collection date

text: CheckM (version 1.1.2)

text: MAG_BA_Lachnospiraceae_13: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

characteristic: description

text: host associated gut microbiome

characteristic: environmental medium

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mouse colon contents

text: host associated gut microbiome

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text: Baseline aging (BA); Aging-associated loss of metabolic host-microbiome-interactions in mice

characteristic: project name

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

text: GTDB-Tk, r214

text: multi-marker approach

characteristic: title


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: No

characteristic: 16s recovered

text: no

characteristic: 23s recovered

text: no

characteristic: 5s recovered

text: CAU

text: public

characteristic: MAG coverage software

characteristic: SRA accession

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:832-115459

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

characteristic: binning parameters

characteristic: binning software

text: host associated gut microbiome

characteristic: completeness score

text: CheckM (version 1.1.2)

characteristic: completeness software

characteristic: contamination score

text: MAG_BA_Acetatifactor_01: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

text: host associated gut microbiome

text: Germany

text: mouse colon contents

text: host associated gut microbiome

text: mouse gut metagenome

characteristic: metagenomic source

characteristic: number of standard tRNAs extracted

text: uncultured Acetatifactor sp.

text: ERS18444878,ERS18444879,ERS18444880,ERS18444881,ERS18444882,ERS18444883,ERS18444884,ERS18444885,ERS18444886,ERS18444887,ERS18444888,ERS18444889,ERS18444890,ERS18444891,ERS18444892,ERS18444893,ERS18444894,ERS18444895,ERS18444896,ERS18444897,ERS18444898,ERS18444899,ERS18444900,ERS18444901,ERS18444902,ERS18444903,ERS18444904,ERS18444905,ERS18444906,ERS18444907,ERS18444908,ERS18444909,ERS18444910,ERS18444911,ERS18444912,ERS18444913,ERS18444914,ERS18444915,ERS18444916,ERS18444917,ERS18444918,ERS18444919,ERS18444920,ERS18444921,ERS18444922,ERS18444923,ERS18444924,ERS18444925,ERS18444926,ERS18444927,ERS18444928,ERS18444929,ERS18590020,ERS18590021,ERS18590022,ERS18590023,ERS18590024,ERS18590025,ERS18590026,ERS18590027

characteristic: sample derived from

text: uncultured Acetatifactor sp.

text: tRNAscan-SE (version 2.0.9)

characteristic: tRNA extraction software

text: GTDB-Tk, r214

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: No

characteristic: 16s recovered

text: no

characteristic: 23s recovered

text: no

characteristic: 5s recovered

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: GC content

text: CAU

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: Bowtie (version 2.2.5) with jgi_summarize_bam_contig_depths script from MetaBAT (version 2.12.1)

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:832-115460

text: host associated gut microbiome

characteristic: broad-scale environmental context

characteristic: collection date

text: CheckM (version 1.1.2)

text: MAG_BA_Acetatifactor_02: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

characteristic: description

text: host associated gut microbiome

characteristic: environmental medium

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mouse colon contents

text: host associated gut microbiome

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Acetatifactor sp.

characteristic: organism

characteristic: project name

text: uncultured Acetatifactor sp.

text: tRNAscan-SE (version 2.0.9)

text: GTDB-Tk, r214

text: multi-marker approach

characteristic: title


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: No

characteristic: 16s recovered

text: yes

characteristic: 23s recovered

text: no

characteristic: 5s recovered

text: CAU

text: public

characteristic: MAG coverage software

characteristic: SRA accession

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:832-115461

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

characteristic: binning parameters

characteristic: binning software

text: host associated gut microbiome

characteristic: completeness score

text: CheckM (version 1.1.2)

characteristic: completeness software

characteristic: contamination score

text: MAG_BA_Acetatifactor_03: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

text: host associated gut microbiome

text: Germany

text: mouse colon contents

text: host associated gut microbiome

text: mouse gut metagenome

characteristic: metagenomic source

characteristic: number of standard tRNAs extracted

text: uncultured Acetatifactor sp.

characteristic: sample derived from

text: uncultured Acetatifactor sp.

text: tRNAscan-SE (version 2.0.9)

characteristic: tRNA extraction software

text: GTDB-Tk, r214

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: No

characteristic: 16s recovered

text: yes

characteristic: 23s recovered

text: no

characteristic: 5s recovered

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: GC content

text: CAU

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:833-115462

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes hybrid (SPAdes version 3.13.1)

text: host associated gut microbiome

characteristic: broad-scale environmental context

characteristic: collection date

text: CheckM (version 1.1.2)

text: MAG_BA_Lachnospiraceae_14: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

characteristic: description

text: host associated gut microbiome

characteristic: environmental medium

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mouse colon contents

text: host associated gut microbiome

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

characteristic: project name

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

text: GTDB-Tk, r214

text: multi-marker approach

characteristic: title


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: Yes

characteristic: 16s recovered

text: yes

characteristic: 23s recovered

text: no

characteristic: 5s recovered

text: CAU

text: public

characteristic: MAG coverage software

characteristic: SRA accession

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:833-115463

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: coverage depth and k-mer binning. Scaffolds were filtered for min. length 1000bp and min. coverage 7.7815. Quality-controlled metagenomic reads were mapped back to the filtered scaffolds with Bowtie (version 2.2.5); the insert size was 0–1000 bp in very sensitive, non-deterministic, “fr” stranded mode with end-to-end alignment. Non-unique mappings and unaligned reads were discarded. The scaffold coverage depth was determined with the jgi_summarize_bam_contig_depths script from MetaBAT (version 2.12.1). Scaffolds were broken into 10 kbp chunks for CONCOCT.

characteristic: binning parameters

text: MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), MaxBin (version 2.2.4), DASTool (version 1.1.2)

characteristic: binning software

text: host associated gut microbiome

characteristic: completeness score

text: CheckM (version 1.1.2)

characteristic: completeness software

characteristic: contamination score

text: MAG_BA_Lachnospiraceae_15: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

text: host associated gut microbiome

text: Germany

text: mouse colon contents

text: host associated gut microbiome

text: mouse gut metagenome

characteristic: metagenomic source

characteristic: number of standard tRNAs extracted

text: uncultured Lachnospiraceae bacterium

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

characteristic: tRNA extraction software

text: GTDB-Tk, r214

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: Yes

characteristic: 16s recovered

text: yes

characteristic: 23s recovered

text: no

characteristic: 5s recovered

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: GC content

text: CAU

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:833-115464

text: host associated gut microbiome

characteristic: broad-scale environmental context

characteristic: collection date

text: CheckM (version 1.1.2)

text: MAG_BA_Lachnospiraceae_16: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

characteristic: description

text: host associated gut microbiome

characteristic: environmental medium

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mouse colon contents

text: host associated gut microbiome

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text: Baseline aging (BA); Aging-associated loss of metabolic host-microbiome-interactions in mice

characteristic: project name

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

text: GTDB-Tk, r214

text: multi-marker approach

characteristic: title


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: No

characteristic: 16s recovered

text: yes

characteristic: 23s recovered

text: no

characteristic: 5s recovered

text: CAU

text: public

characteristic: MAG coverage software

characteristic: SRA accession

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:833-115465

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

characteristic: binning parameters

characteristic: binning software

text: host associated gut microbiome

characteristic: completeness score

text: CheckM (version 1.1.2)

characteristic: completeness software

characteristic: contamination score

text: MAG_BA_Lachnospiraceae_17: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

text: host associated gut microbiome

text: Germany

text: mouse colon contents

text: host associated gut microbiome

text: mouse gut metagenome

characteristic: metagenomic source

characteristic: number of standard tRNAs extracted

text: uncultured Lachnospiraceae bacterium

text: ERS18444878,ERS18444879,ERS18444880,ERS18444881,ERS18444882,ERS18444883,ERS18444884,ERS18444885,ERS18444886,ERS18444887,ERS18444888,ERS18444889,ERS18444890,ERS18444891,ERS18444892,ERS18444893,ERS18444894,ERS18444895,ERS18444896,ERS18444897,ERS18444898,ERS18444899,ERS18444900,ERS18444901,ERS18444902,ERS18444903,ERS18444904,ERS18444905,ERS18444906,ERS18444907,ERS18444908,ERS18444909,ERS18444910,ERS18444911,ERS18444912,ERS18444913,ERS18444914,ERS18444915,ERS18444916,ERS18444917,ERS18444918,ERS18444919,ERS18444920,ERS18444921,ERS18444922,ERS18444923,ERS18444924,ERS18444925,ERS18444926,ERS18444927,ERS18444928,ERS18444929,ERS18590020,ERS18590021,ERS18590022,ERS18590023,ERS18590024,ERS18590025,ERS18590026,ERS18590027

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

characteristic: tRNA extraction software

text: GTDB-Tk, r214

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: Yes

characteristic: 16s recovered

text: yes

characteristic: 23s recovered

text: no

characteristic: 5s recovered

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: GC content

text: CAU

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: Bowtie (version 2.2.5) with jgi_summarize_bam_contig_depths script from MetaBAT (version 2.12.1)

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:833-115466

text: host associated gut microbiome

characteristic: broad-scale environmental context

characteristic: collection date

text: CheckM (version 1.1.2)

text: MAG_BA_Lachnospiraceae_18: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

characteristic: description

text: host associated gut microbiome

characteristic: environmental medium

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mouse colon contents

text: host associated gut microbiome

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

characteristic: project name

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

text: GTDB-Tk, r214

text: multi-marker approach

characteristic: title


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: No

characteristic: 16s recovered

text: yes

characteristic: 23s recovered

text: no

characteristic: 5s recovered

text: CAU

text: public

characteristic: MAG coverage software

characteristic: SRA accession

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:834-115467

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

characteristic: binning parameters

characteristic: binning software

text: host associated gut microbiome

characteristic: completeness score

text: CheckM (version 1.1.2)

characteristic: completeness software

characteristic: contamination score

text: MAG_BA_Lachnospiraceae_19: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

text: host associated gut microbiome

text: Germany

text: mouse colon contents

text: host associated gut microbiome

text: mouse gut metagenome

characteristic: metagenomic source

characteristic: number of standard tRNAs extracted

text: uncultured Lachnospiraceae bacterium

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

characteristic: tRNA extraction software

text: GTDB-Tk, r214

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: Yes

characteristic: 16s recovered

text: yes

characteristic: 23s recovered

text: no

characteristic: 5s recovered

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: GC content

text: CAU

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:834-115468

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes hybrid (SPAdes version 3.13.1)

text: host associated gut microbiome

characteristic: broad-scale environmental context

characteristic: collection date

text: CheckM (version 1.1.2)

text: MAG_BA_Lachnospiraceae_20: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

characteristic: description

text: host associated gut microbiome

characteristic: environmental medium

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mouse colon contents

text: host associated gut microbiome

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

characteristic: project name

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

text: GTDB-Tk, r214

text: multi-marker approach

characteristic: title


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: Yes

characteristic: 16s recovered

text: no

characteristic: 23s recovered

text: no

characteristic: 5s recovered

text: CAU

text: public

characteristic: MAG coverage software

characteristic: SRA accession

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:834-115469

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: coverage depth and k-mer binning. Scaffolds were filtered for min. length 1000bp and min. coverage 7.7815. Quality-controlled metagenomic reads were mapped back to the filtered scaffolds with Bowtie (version 2.2.5); the insert size was 0–1000 bp in very sensitive, non-deterministic, “fr” stranded mode with end-to-end alignment. Non-unique mappings and unaligned reads were discarded. The scaffold coverage depth was determined with the jgi_summarize_bam_contig_depths script from MetaBAT (version 2.12.1). Scaffolds were broken into 10 kbp chunks for CONCOCT.

characteristic: binning parameters

text: MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), MaxBin (version 2.2.4), DASTool (version 1.1.2)

characteristic: binning software

text: host associated gut microbiome

characteristic: completeness score

text: CheckM (version 1.1.2)

characteristic: completeness software

characteristic: contamination score

text: MAG_BA_Lachnospiraceae_21: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

text: host associated gut microbiome

text: Germany

text: mouse colon contents

text: host associated gut microbiome

text: mouse gut metagenome

characteristic: metagenomic source

characteristic: number of standard tRNAs extracted

text: uncultured Lachnospiraceae bacterium

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

characteristic: tRNA extraction software

text: GTDB-Tk, r214

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: No

characteristic: 16s recovered

text: yes

characteristic: 23s recovered

text: no

characteristic: 5s recovered

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: GC content

text: CAU

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:834-115470

text: host associated gut microbiome

characteristic: broad-scale environmental context

characteristic: collection date

text: CheckM (version 1.1.2)

text: MAG_BA_Lachnospiraceae_22: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

characteristic: description

text: host associated gut microbiome

characteristic: environmental medium

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mouse colon contents

text: host associated gut microbiome

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text: Baseline aging (BA); Aging-associated loss of metabolic host-microbiome-interactions in mice

characteristic: project name

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

text: GTDB-Tk, r214

text: multi-marker approach

characteristic: title


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: No

characteristic: 16s recovered

text: no

characteristic: 23s recovered

text: no

characteristic: 5s recovered

text: CAU

text: public

characteristic: MAG coverage software

characteristic: SRA accession

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:835-115471

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

characteristic: binning parameters

characteristic: binning software

text: host associated gut microbiome

characteristic: completeness score

text: CheckM (version 1.1.2)

characteristic: completeness software

characteristic: contamination score

text: MAG_BA_Lachnospiraceae_23: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

text: host associated gut microbiome

text: Germany

text: mouse colon contents

text: host associated gut microbiome

text: mouse gut metagenome

characteristic: metagenomic source

characteristic: number of standard tRNAs extracted

text: uncultured Lachnospiraceae bacterium

text: ERS18444878,ERS18444879,ERS18444880,ERS18444881,ERS18444882,ERS18444883,ERS18444884,ERS18444885,ERS18444886,ERS18444887,ERS18444888,ERS18444889,ERS18444890,ERS18444891,ERS18444892,ERS18444893,ERS18444894,ERS18444895,ERS18444896,ERS18444897,ERS18444898,ERS18444899,ERS18444900,ERS18444901,ERS18444902,ERS18444903,ERS18444904,ERS18444905,ERS18444906,ERS18444907,ERS18444908,ERS18444909,ERS18444910,ERS18444911,ERS18444912,ERS18444913,ERS18444914,ERS18444915,ERS18444916,ERS18444917,ERS18444918,ERS18444919,ERS18444920,ERS18444921,ERS18444922,ERS18444923,ERS18444924,ERS18444925,ERS18444926,ERS18444927,ERS18444928,ERS18444929,ERS18590020,ERS18590021,ERS18590022,ERS18590023,ERS18590024,ERS18590025,ERS18590026,ERS18590027

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

characteristic: tRNA extraction software

text: GTDB-Tk, r214

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: No

characteristic: 16s recovered

text: no

characteristic: 23s recovered

text: no

characteristic: 5s recovered

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: GC content

text: CAU

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: Bowtie (version 2.2.5) with jgi_summarize_bam_contig_depths script from MetaBAT (version 2.12.1)

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:835-115472

text: host associated gut microbiome

characteristic: broad-scale environmental context

characteristic: collection date

text: CheckM (version 1.1.2)

text: MAG_BA_Lachnospiraceae_24: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

characteristic: description

text: host associated gut microbiome

characteristic: environmental medium

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mouse colon contents

text: host associated gut microbiome

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

characteristic: project name

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

text: GTDB-Tk, r214

text: multi-marker approach

characteristic: title


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: Yes

characteristic: 16s recovered

text: no

characteristic: 23s recovered

text: no

characteristic: 5s recovered

text: CAU

text: public

characteristic: MAG coverage software

characteristic: SRA accession

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:835-115473

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

characteristic: binning parameters

characteristic: binning software

text: host associated gut microbiome

characteristic: completeness score

text: CheckM (version 1.1.2)

characteristic: completeness software

characteristic: contamination score

text: MAG_BA_Kineothrix_01: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

text: host associated gut microbiome

text: Germany

text: mouse colon contents

text: host associated gut microbiome

text: mouse gut metagenome

characteristic: metagenomic source

characteristic: number of standard tRNAs extracted

text: uncultured Kineothrix sp.

characteristic: sample derived from

text: uncultured Kineothrix sp.

text: tRNAscan-SE (version 2.0.9)

characteristic: tRNA extraction software

text: GTDB-Tk, r214

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: No

characteristic: 16s recovered

text: yes

characteristic: 23s recovered

text: no

characteristic: 5s recovered

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: GC content

text: CAU

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:835-115474

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes hybrid (SPAdes version 3.13.1)

text: host associated gut microbiome

characteristic: broad-scale environmental context

characteristic: collection date

text: CheckM (version 1.1.2)

text: MAG_BA_Kineothrix_02: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

characteristic: description

text: host associated gut microbiome

characteristic: environmental medium

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mouse colon contents

text: host associated gut microbiome

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Kineothrix sp.

characteristic: organism

characteristic: project name

text: uncultured Kineothrix sp.

text: tRNAscan-SE (version 2.0.9)

text: GTDB-Tk, r214

text: multi-marker approach

characteristic: title


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: Yes

characteristic: 16s recovered

text: no

characteristic: 23s recovered

text: no

characteristic: 5s recovered

text: CAU

text: public

characteristic: MAG coverage software

characteristic: SRA accession

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:835-115475

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: coverage depth and k-mer binning. Scaffolds were filtered for min. length 1000bp and min. coverage 7.7815. Quality-controlled metagenomic reads were mapped back to the filtered scaffolds with Bowtie (version 2.2.5); the insert size was 0–1000 bp in very sensitive, non-deterministic, “fr” stranded mode with end-to-end alignment. Non-unique mappings and unaligned reads were discarded. The scaffold coverage depth was determined with the jgi_summarize_bam_contig_depths script from MetaBAT (version 2.12.1). Scaffolds were broken into 10 kbp chunks for CONCOCT.

characteristic: binning parameters

text: MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), MaxBin (version 2.2.4), DASTool (version 1.1.2)

characteristic: binning software

text: host associated gut microbiome

characteristic: completeness score

text: CheckM (version 1.1.2)

characteristic: completeness software

characteristic: contamination score

text: MAG_BA_Lachnospiraceae_25: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

text: host associated gut microbiome

text: Germany

text: mouse colon contents

text: host associated gut microbiome

text: mouse gut metagenome

characteristic: metagenomic source

characteristic: number of standard tRNAs extracted

text: uncultured Lachnospiraceae bacterium

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

characteristic: tRNA extraction software

text: GTDB-Tk, r214

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: Yes

characteristic: 16s recovered

text: no

characteristic: 23s recovered

text: no

characteristic: 5s recovered

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: GC content

text: CAU

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:836-115476

text: host associated gut microbiome

characteristic: broad-scale environmental context

characteristic: collection date

text: CheckM (version 1.1.2)

text: MAG_BA_Lachnospiraceae_26: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

characteristic: description

text: host associated gut microbiome

characteristic: environmental medium

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mouse colon contents

text: host associated gut microbiome

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text: Baseline aging (BA); Aging-associated loss of metabolic host-microbiome-interactions in mice

characteristic: project name

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

text: GTDB-Tk, r214

text: multi-marker approach

characteristic: title


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: Yes

characteristic: 16s recovered

text: yes

characteristic: 23s recovered

text: no

characteristic: 5s recovered

text: CAU

text: public

characteristic: MAG coverage software

characteristic: SRA accession

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:836-115477

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

characteristic: binning parameters

characteristic: binning software

text: host associated gut microbiome

characteristic: completeness score

text: CheckM (version 1.1.2)

characteristic: completeness software

characteristic: contamination score

text: MAG_BA_Ventrimonas_01: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

text: host associated gut microbiome

text: Germany

text: mouse colon contents

text: host associated gut microbiome

text: mouse gut metagenome

characteristic: metagenomic source

characteristic: number of standard tRNAs extracted

text: uncultured Lachnospiraceae bacterium

text: ERS18444878,ERS18444879,ERS18444880,ERS18444881,ERS18444882,ERS18444883,ERS18444884,ERS18444885,ERS18444886,ERS18444887,ERS18444888,ERS18444889,ERS18444890,ERS18444891,ERS18444892,ERS18444893,ERS18444894,ERS18444895,ERS18444896,ERS18444897,ERS18444898,ERS18444899,ERS18444900,ERS18444901,ERS18444902,ERS18444903,ERS18444904,ERS18444905,ERS18444906,ERS18444907,ERS18444908,ERS18444909,ERS18444910,ERS18444911,ERS18444912,ERS18444913,ERS18444914,ERS18444915,ERS18444916,ERS18444917,ERS18444918,ERS18444919,ERS18444920,ERS18444921,ERS18444922,ERS18444923,ERS18444924,ERS18444925,ERS18444926,ERS18444927,ERS18444928,ERS18444929,ERS18590020,ERS18590021,ERS18590022,ERS18590023,ERS18590024,ERS18590025,ERS18590026,ERS18590027

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

characteristic: tRNA extraction software

text: GTDB-Tk, r214

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: No

characteristic: 16s recovered

text: no

characteristic: 23s recovered

text: no

characteristic: 5s recovered

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: GC content

text: CAU

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: Bowtie (version 2.2.5) with jgi_summarize_bam_contig_depths script from MetaBAT (version 2.12.1)

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:836-115478

text: host associated gut microbiome

characteristic: broad-scale environmental context

characteristic: collection date

text: CheckM (version 1.1.2)

text: MAG_BA_Ventrimonas_02: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

characteristic: description

text: host associated gut microbiome

characteristic: environmental medium

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mouse colon contents

text: host associated gut microbiome

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

characteristic: project name

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

text: GTDB-Tk, r214

text: multi-marker approach

characteristic: title


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: Yes

characteristic: 16s recovered

text: yes

characteristic: 23s recovered

text: yes

characteristic: 5s recovered

text: CAU

text: public

characteristic: MAG coverage software

characteristic: SRA accession

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:836-115479

characteristic: Submitter Id

text: Multiple fragments where gaps span repetitive regions. Presence of the 23S, 16S, and 5S rRNA genes and at least 18 tRNAs

characteristic: assembly quality

characteristic: assembly software

characteristic: binning parameters

characteristic: binning software

text: host associated gut microbiome

characteristic: completeness score

text: CheckM (version 1.1.2)

characteristic: completeness software

characteristic: contamination score

text: MAG_BA_Lachnospira_01: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

text: host associated gut microbiome

text: Germany

text: mouse colon contents

text: host associated gut microbiome

text: mouse gut metagenome

characteristic: metagenomic source

characteristic: number of standard tRNAs extracted

text: uncultured Lachnospira sp.

characteristic: sample derived from

text: uncultured Lachnospira sp.

text: tRNAscan-SE (version 2.0.9)

characteristic: tRNA extraction software

text: GTDB-Tk, r214

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: Yes

characteristic: 16s recovered

text: no

characteristic: 23s recovered

text: no

characteristic: 5s recovered

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: GC content

text: CAU

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:837-115480

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes hybrid (SPAdes version 3.13.1)

text: host associated gut microbiome

characteristic: broad-scale environmental context

characteristic: collection date

text: CheckM (version 1.1.2)

text: MAG_BA_Lachnospiraceae_27: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

characteristic: description

text: host associated gut microbiome

characteristic: environmental medium

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mouse colon contents

text: host associated gut microbiome

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

characteristic: project name

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

text: GTDB-Tk, r214

text: multi-marker approach

characteristic: title


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: Yes

characteristic: 16s recovered

text: no

characteristic: 23s recovered

text: yes

characteristic: 5s recovered

text: CAU

text: public

characteristic: MAG coverage software

characteristic: SRA accession

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:837-115481

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: coverage depth and k-mer binning. Scaffolds were filtered for min. length 1000bp and min. coverage 7.7815. Quality-controlled metagenomic reads were mapped back to the filtered scaffolds with Bowtie (version 2.2.5); the insert size was 0–1000 bp in very sensitive, non-deterministic, “fr” stranded mode with end-to-end alignment. Non-unique mappings and unaligned reads were discarded. The scaffold coverage depth was determined with the jgi_summarize_bam_contig_depths script from MetaBAT (version 2.12.1). Scaffolds were broken into 10 kbp chunks for CONCOCT.

characteristic: binning parameters

text: MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), MaxBin (version 2.2.4), DASTool (version 1.1.2)

characteristic: binning software

text: host associated gut microbiome

characteristic: completeness score

text: CheckM (version 1.1.2)

characteristic: completeness software

characteristic: contamination score

text: MAG_BA_Lachnospiraceae_28: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

text: host associated gut microbiome

text: Germany

text: mouse colon contents

text: host associated gut microbiome

text: mouse gut metagenome

characteristic: metagenomic source

characteristic: number of standard tRNAs extracted

text: uncultured Lachnospiraceae bacterium

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

characteristic: tRNA extraction software

text: GTDB-Tk, r214

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: Yes

characteristic: 16s recovered

text: no

characteristic: 23s recovered

text: no

characteristic: 5s recovered

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: GC content

text: CAU

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:837-115482

text: host associated gut microbiome

characteristic: broad-scale environmental context

characteristic: collection date

text: CheckM (version 1.1.2)

text: MAG_BA_Lachnospiraceae_29: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

characteristic: description

text: host associated gut microbiome

characteristic: environmental medium

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mouse colon contents

text: host associated gut microbiome

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text: Baseline aging (BA); Aging-associated loss of metabolic host-microbiome-interactions in mice

characteristic: project name

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

text: GTDB-Tk, r214

text: multi-marker approach

characteristic: title


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: Yes

characteristic: 16s recovered

text: yes

characteristic: 23s recovered

text: no

characteristic: 5s recovered

text: CAU

text: public

text: Bowtie (version 2.2.5) with jgi_summarize_bam_contig_depths script from MetaBAT (version 2.12.1)

characteristic: MAG coverage software

characteristic: SRA accession

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:837-115483

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

characteristic: binning parameters

characteristic: binning software

text: host associated gut microbiome

characteristic: completeness score

text: CheckM (version 1.1.2)

characteristic: completeness software

characteristic: contamination score

text: MAG_BA_Eubacterium_01: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

text: host associated gut microbiome

text: Germany

text: mouse colon contents

text: host associated gut microbiome

text: mouse gut metagenome

characteristic: metagenomic source

characteristic: number of standard tRNAs extracted

text: uncultured Lachnospiraceae bacterium

text: ERS18444878,ERS18444879,ERS18444880,ERS18444881,ERS18444882,ERS18444883,ERS18444884,ERS18444885,ERS18444886,ERS18444887,ERS18444888,ERS18444889,ERS18444890,ERS18444891,ERS18444892,ERS18444893,ERS18444894,ERS18444895,ERS18444896,ERS18444897,ERS18444898,ERS18444899,ERS18444900,ERS18444901,ERS18444902,ERS18444903,ERS18444904,ERS18444905,ERS18444906,ERS18444907,ERS18444908,ERS18444909,ERS18444910,ERS18444911,ERS18444912,ERS18444913,ERS18444914,ERS18444915,ERS18444916,ERS18444917,ERS18444918,ERS18444919,ERS18444920,ERS18444921,ERS18444922,ERS18444923,ERS18444924,ERS18444925,ERS18444926,ERS18444927,ERS18444928,ERS18444929,ERS18590020,ERS18590021,ERS18590022,ERS18590023,ERS18590024,ERS18590025,ERS18590026,ERS18590027

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

characteristic: tRNA extraction software

text: GTDB-Tk, r214

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: No

characteristic: 16s recovered

text: no

characteristic: 23s recovered

text: no

characteristic: 5s recovered

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: GC content

text: CAU

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:837-115484

text: host associated gut microbiome

characteristic: broad-scale environmental context

characteristic: collection date

text: CheckM (version 1.1.2)

text: MAG_BA_Butyribacter_01: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

characteristic: description

text: host associated gut microbiome

characteristic: environmental medium

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mouse colon contents

text: host associated gut microbiome

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

characteristic: project name

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

text: GTDB-Tk, r214

text: multi-marker approach

characteristic: title


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: Yes

characteristic: 16s recovered

text: yes

characteristic: 23s recovered

text: no

characteristic: 5s recovered

text: CAU

text: public

characteristic: MAG coverage software

characteristic: SRA accession

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:838-115485

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

characteristic: binning parameters

characteristic: binning software

text: host associated gut microbiome

characteristic: completeness score

text: CheckM (version 1.1.2)

characteristic: completeness software

characteristic: contamination score

text: MAG_BA_Butyribacter_02: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

text: host associated gut microbiome

text: Germany

text: mouse colon contents

text: host associated gut microbiome

text: mouse gut metagenome

characteristic: metagenomic source

characteristic: number of standard tRNAs extracted

text: uncultured Lachnospiraceae bacterium

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

characteristic: tRNA extraction software

text: GTDB-Tk, r214

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: Yes

characteristic: 16s recovered

text: yes

characteristic: 23s recovered

text: no

characteristic: 5s recovered

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: GC content

text: CAU

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:838-115486

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes hybrid (SPAdes version 3.13.1)

text: host associated gut microbiome

characteristic: broad-scale environmental context

characteristic: collection date

text: CheckM (version 1.1.2)

text: MAG_BA_Butyribacter_03: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

characteristic: description

text: host associated gut microbiome

characteristic: environmental medium

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mouse colon contents

text: host associated gut microbiome

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

characteristic: project name

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

text: GTDB-Tk, r214

text: multi-marker approach

characteristic: title


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: No

characteristic: 16s recovered

text: yes

characteristic: 23s recovered

text: no

characteristic: 5s recovered

text: CAU

text: public

characteristic: MAG coverage software

characteristic: SRA accession

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:838-115487

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: coverage depth and k-mer binning. Scaffolds were filtered for min. length 1000bp and min. coverage 7.7815. Quality-controlled metagenomic reads were mapped back to the filtered scaffolds with Bowtie (version 2.2.5); the insert size was 0–1000 bp in very sensitive, non-deterministic, “fr” stranded mode with end-to-end alignment. Non-unique mappings and unaligned reads were discarded. The scaffold coverage depth was determined with the jgi_summarize_bam_contig_depths script from MetaBAT (version 2.12.1). Scaffolds were broken into 10 kbp chunks for CONCOCT.

characteristic: binning parameters

text: MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), MaxBin (version 2.2.4), DASTool (version 1.1.2)

characteristic: binning software

text: host associated gut microbiome

characteristic: completeness score

text: CheckM (version 1.1.2)

characteristic: completeness software

characteristic: contamination score

text: MAG_BA_Butyribacter_04: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

text: host associated gut microbiome

text: Germany

text: mouse colon contents

text: host associated gut microbiome

text: mouse gut metagenome

characteristic: metagenomic source

characteristic: number of standard tRNAs extracted

text: uncultured Lachnospiraceae bacterium

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

characteristic: tRNA extraction software

text: GTDB-Tk, r214

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: Yes

characteristic: 16s recovered

text: yes

characteristic: 23s recovered

text: yes

characteristic: 5s recovered

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: GC content

text: CAU

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:838-115488

text: Multiple fragments where gaps span repetitive regions. Presence of the 23S, 16S, and 5S rRNA genes and at least 18 tRNAs

text: host associated gut microbiome

characteristic: broad-scale environmental context

characteristic: collection date

text: CheckM (version 1.1.2)

text: MAG_BA_Lachnospiraceae_30: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

characteristic: description

text: host associated gut microbiome

characteristic: environmental medium

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mouse colon contents

text: host associated gut microbiome

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text: Baseline aging (BA); Aging-associated loss of metabolic host-microbiome-interactions in mice

characteristic: project name

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

text: GTDB-Tk, r214

text: multi-marker approach

characteristic: title


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: Yes

characteristic: 16s recovered

text: yes

characteristic: 23s recovered

text: yes

characteristic: 5s recovered

text: CAU

text: public

text: Bowtie (version 2.2.5) with jgi_summarize_bam_contig_depths script from MetaBAT (version 2.12.1)

characteristic: MAG coverage software

characteristic: SRA accession

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:838-115489

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

characteristic: binning parameters

characteristic: binning software

text: host associated gut microbiome

characteristic: completeness score

text: CheckM (version 1.1.2)

characteristic: completeness software

characteristic: contamination score

text: MAG_BA_Wujia_01: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

text: host associated gut microbiome

text: Germany

text: mouse colon contents

text: host associated gut microbiome

text: mouse gut metagenome

characteristic: metagenomic source

characteristic: number of standard tRNAs extracted

text: uncultured Lachnospiraceae bacterium

text: ERS18444878,ERS18444879,ERS18444880,ERS18444881,ERS18444882,ERS18444883,ERS18444884,ERS18444885,ERS18444886,ERS18444887,ERS18444888,ERS18444889,ERS18444890,ERS18444891,ERS18444892,ERS18444893,ERS18444894,ERS18444895,ERS18444896,ERS18444897,ERS18444898,ERS18444899,ERS18444900,ERS18444901,ERS18444902,ERS18444903,ERS18444904,ERS18444905,ERS18444906,ERS18444907,ERS18444908,ERS18444909,ERS18444910,ERS18444911,ERS18444912,ERS18444913,ERS18444914,ERS18444915,ERS18444916,ERS18444917,ERS18444918,ERS18444919,ERS18444920,ERS18444921,ERS18444922,ERS18444923,ERS18444924,ERS18444925,ERS18444926,ERS18444927,ERS18444928,ERS18444929,ERS18590020,ERS18590021,ERS18590022,ERS18590023,ERS18590024,ERS18590025,ERS18590026,ERS18590027

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: tRNAscan-SE (version 2.0.9)

characteristic: tRNA extraction software

text: GTDB-Tk, r214

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: No

characteristic: 16s recovered

text: yes

characteristic: 23s recovered

text: yes

characteristic: 5s recovered

characteristic: ENA first public

characteristic: ENA-CHECKLIST

characteristic: GC content

text: CAU

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:839-115490

text: host associated gut microbiome

characteristic: broad-scale environmental context

characteristic: collection date

text: CheckM (version 1.1.2)

text: MAG_BA_Anaerotignum_01: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

characteristic: description

text: host associated gut microbiome

characteristic: environmental medium

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mouse colon contents

text: host associated gut microbiome

characteristic: local environmental context

text: mouse gut metagenome

text: uncultured Anaerotignum sp.

characteristic: organism

characteristic: project name

text: uncultured Anaerotignum sp.

text: tRNAscan-SE (version 2.0.9)

text: GTDB-Tk, r214

text: multi-marker approach

characteristic: title


text: barrnap (version 0.9)

characteristic: 16S recovery software

text: No

characteristic: 16s recovered

text: no

characteristic: 23s recovered

text: yes

characteristic: 5s recovered

text: CAU

text: public

characteristic: MAG coverage software

characteristic: SRA accession

text: ena-SAMPLE-TAB-22-03-2024-10:43:23:839-115491

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

characteristic: assembly software

characteristic: binning parameters

characteristic: binning software

text: host associated gut microbiome

characteristic: completeness score

text: CheckM (version 1.1.2)

characteristic: completeness software

characteristic: contamination score

text: MAG_BA_Acutalibacter_01: Metagenomic assembled genome (MAG) from colon contents of n=52, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with metaSpades hybrid. Coverage and k-mer binning with MetaBAT (version 2.12.1), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced 252 bins which were refined into 181, high to medium quality MAGs via DASTool.

text: host associated gut microbiome

text: Germany

text: mouse colon contents

text: host associated gut microbiome

text: mouse gut metagenome

characteristic: metagenomic source

characteristic: number of standard tRNAs extracted

text: uncultured Acutalibacter sp.

characteristic: sample derived from

text: uncultured Acutalibacter sp.

text: tRNAscan-SE (version 2.0.9)

characteristic: tRNA extraction software

text: GTDB-Tk, r214

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker