title: Paramecium bursaria Chlorella Virus-1 (PBCV-1) oligo array, v.1.0
status: Public on Jan 10 2010
contact.city: Lincoln
contact.first: James
contact.middle: L
contact.last: Van Etten
contact.email: jvanetten@unlnotes.unl.edu
contact.state: NE
contact.address: 4240 Fair Street
contact.department: Plant Pathology
contact.country: USA
contact.institute: University of Nebraska-Lincoln
contact.phone: 402-472-3168
organism: Paramecium bursaria Chlorella virus 1
description: The Platform data table reflects a condensed representation of the array's replicate features.
manufacture_protocol: 50-mer probes representing each major PBCV-1 protein encoding gene (CDS) were designed and synthesized by MWG Biotech (Ebersberg, Germany) (20-80% GC, 60-80º C melting temperature).  Probes were spotted onto CMT-GAPS silane-coated slides (Corning, Lowell, MA) using Omnigrid 100 (Genomic Solutions, Ann Arbor, MI) according to manufacturer’s instructions.  Probes were printed in quadruplicates on every slide.