title: IHB miR Zebrafish 14 0.3K
status: Public on Sep 17 2012
contact.city: Wuhan
contact.first: Yanyan
contact.last:  Zhao
contact.email: yan_zi1104@126.com
contact.address: Donghu South Road 7
contact.country: China
contact.institute: Institute of Hydrobiology
organism: Danio rerio
manufacture_protocol: Arrays are made based µParaflo microfluidic technology (Atactic Technologies). On the µParaflo microfluidic chip, each detection probe consists of a chemically modified nucleotide coding segment complementary to target microRNA (from miRBase, http://microrna.sanger.ac.uk/sequences/) or other RNA (control or customer defined sequences) and a spacer segment of polyethylene glycol to extend the coding segment away from the substrate. The detection probes are made by in situ synthesis using PGR (photogenerated reagent) chemistry.