title: Breakthrough Human 17K 2.1.2
status: Public on Aug 30 2014
contact.city: London
contact.first: Richard
contact.middle: Dafydd
contact.last: Williams
contact.email: richard.williams@ucl.ac.uk
contact.address: 30 Guilford Street
contact.department: Developmental Biology and Cancer
contact.country: United Kingdom
contact.institute: UCL Institute of Child Health
organism: Homo sapiens
manufacture_protocol: IMAGE clones in bacterial glycerol stocks were acquired from the CRUK DNA microarray facility at the Institute of Cancer Research, Sutton, UK, and from MRC Geneservice, Cambridge, UK. Clones were PCR-amplified using YieldAce DNA Polymerase (Stratagene) and primers: KIMF (GAGCGGATAACAATTTCACACAG) and KIMR (GTTTTCCCAGTCACGACG) for ampicillin resistant clones, or PM001a (GTCGACGTTAGAACGCGGCTAC) and PM002a (GGGTTAAATTCCCGGGTACTGC) for chloramphenicol resistant clones. PCR products were purified using the Montage 96-well purification plate system (Millipore). Purified DNA was mixed with equal volumes of 2x Microarray Spotting Solution (Genetix), and printed on FMB cDNA slides (Full Moon Biosystems) using a Lucidea ArraySpotter (Amersham). ). The array was laid out in 24 blocks (2 columns, 12 rows). Each block contained 748 features (17 rows, 44 columns, Pitch 190 micrometre). [Adapted from A. Grigoriadis, ArrayExpress A-MEXP-347].