title: Borrelia burgdorferi 70-mer oligoarray
status: Public on Sep 09 2008
contact.city: Dallas
contact.first: zhiming
contact.last: ouyang
contact.email: zhiming.ouyang@UTSouthwestern.edu
contact.state: TX
contact.address: 6000 Harry Hines Blvd
contact.department: Department of Microbiology
contact.country: USA
contact.institute: UT Southwestern Medical Center
contact.phone: 214-648-5983
organism: Borreliella burgdorferi B31
manufacture_protocol: Oligonucleotides representing all annotated ORFs and pseudogenes of B. burgdorferi B31MI in the TIGR or NCBI genome databases were synthesized by QIAGEN-Operon (Alameda, CA) according to a proprietary algorithm that optimizes the melting temperature (Tm) for all oligonucleotides within 5°C of each other. The target Tm was 70°C, and only five oligonucleotides (BB0150, BB0609, BBH39, BBK001, and BBQ83) failed to have a minimum Tm of 65°C. All oligonucleotides are 70 nucleotides in length and are at least 40 nucleotides from the 3' end of the ORF sequence (except for a number of short ORFs, where these criteria could not be met). In addition, 19 random-sequence 70-mers were synthesized as negative controls.