title: LMIVV laboratory, Animal Pan-viral DNA array, 3800 probes, V2
status: Public on Nov 02 2015
contact.city: St-Hyacinthe
contact.first: Christian
contact.last: Bellehumeur
contact.email: christianbellehumeur@yahoo.ca
contact.address: 3200 Sicotte
contact.country: Canada
contact.institute: Université de Montréal
organism: Viruses
description: multiple animal viruses DNA or RNA genomic sequences selected from sequences alignment
manufacture_protocol: Lyophilized oligonucleotides were suspended in water to a concentration of 50 pmol/ml. These probe stocks were diluted in dimethyl sulfoxide (50%, v/v) to a final concentration of 25 pmol/ml. Ten ml of each probe was transferred into a 384-well microplate and printed onto Corning GAPS II slides (Corning Co., Corning, N.Y.) with a Virtek ChipWriter, using Telechem SMP3 microspotting pins. Each probe was printed in duplicate. Slides were processed by UV cross-linking (600 mJ) followed by heat treatment (80 C overnight) and stored in the dark at room temperature until use.