title: QuantiGene 2.0 Multiplex Assay [Custom Mouse Panel]
status: Public on Jan 09 2015
contact.city: St. Louis
contact.first: Muhammad Farooq
contact.last: Rai
contact.state: MO
contact.address: 660 S. Euclid Ave.
contact.department: Orthopaedic Surgery
contact.country: USA
contact.institute: Washington University School of Medicine
organism: Mus musculus
description: The QuantiGene Plex 2.0 Assay is a hybridization-based assay using the xMAP® Luminex® magnetic beads and performed on 96-well plates. The assay is based on DIRECT quantification of the RNA targets using xMAP Luminex beads for multiplexing of 3 to 80 RNA targets and branched DNA (bDNA) signal amplification technology. On the first day the sample is lysed to release the RNAs, and incubated overnight with target specific probe sets panel. On the second day the signal amplification tree is build via sequential hybridization of PreAmplifier (PreAmp), Amplifier (Amp) and Label Probe (LP). Each amplification unit gives a 400 x signal amplification and there are six amplification units per target RNA copy leaning to a 2,400 x signal amplification per copy RNA. The signal is detected by adding SAPE substrate and using a Luminex instrument for the read out.
manufacture_protocol: see manufacturer's website (http://www.panomics.com/products/gene-expression/plex-2.0-assay/overview)