title: Thermoanaerobacter sp. X514 2.3K oligo array version 1.0
status: Public on Feb 01 2011
contact.city: Qingdao
contact.first: Lu
contact.last: Lin
contact.email: linlu623@yahoo.com.cn
contact.state: Shandong
contact.address: 189 Songling RD
contact.country: China
contact.institute: QIBEBT
organism: Thermoanaerobacter sp. X514
manufacture_protocol: All designed oligonucleotides were commercially synthesized without modification by Invitrogen.
  Oligonucleotide probes were prepared in 50% dimethyl sulfoxide (Sigma Chemical Co., MO).
  (http://ieg.ou.edu/protocol/Protocols/DMSO_Printing_8.pdf)
  The concentration of oligonucleotide probes was adjusted to 100pmol/L. were spotted onto SuperAmine glass slides (Telechem International, CA) using a PixSys 5500 robotic printer (CartesianTechnologies Inc., CA).
  Each probe had two replicates on a single slide.
  After printing, the oligonucleotide probes were fixed onto the slides by UV cross-linking (600 mJ of energy) according to the protocol of the manufacturer (Telechem International, CA). (http://aem.asm.org/cgi/reprint/71/7/3753)