title: 2D-LC MS/MS Analyses of HLA Class I Peptides Isolated from B-cells Infected with Vaccinia Virus
status: Public on Aug 31 2009
contact.city: Rochester
contact.first: Kenneth
contact.middle: L.
contact.last: Johnson
contact.email: johnson.kenneth@mayo.edu
contact.state: MN
contact.address: 200 First Street S.W.
contact.country: USA
contact.institute: Mayo Clinic
organism: Homo sapiens
  Bos taurus
  Orthopoxvirus vaccinia
description: B-cell cultures were infected with live vaccinia virus (NYBOH strain).  Cells were lysed, HLA/peptide complexes were enriched by immunoaffinity (W6/32 mAb), peptides were released with acid and separated from HLA proteins by 10 kDa ultrafilitration.  HLA class I peptides were separated and identified by 2-dimensional liquid chromatography and tandem mass spectrometry to identify class I peptides derived from vaccinia proteins, that were being presented to the immune system.