title: PDP Mouse 16K BMAP-MSV
status: Public on Aug 27 2008
contact.city: Winnipeg
contact.first: Debra
contact.last: Parchaliuk
contact.email: Debra_Parchaliuk@phac-aspc.gc.ca
contact.state: Manitoba
contact.address: 1015 Arlington Street
contact.department: Prion Diseases Program
contact.country: Canada
contact.institute: Public Health Agency of Canada
contact.phone: 2047895014
organism: Mus musculus
manufacture_protocol: Libraries and clones derived from brain regions of adult mouse (C57BL/6) were obtained from Research Genetics. The 11 136 clones represent expressed sequence tags (ESTs) from some 5471 unique known genes and 5665 as yet uncharacterized mRNAs and form part of a library that was created as part of the Brain Molecular Anatomy Project (BMAP). In addition, a Mouse Sequence Verified (MSV) Unigene library, containing 5179  ESTs was added to the array. Each insert from these 3' EST libraries was amplified by PCR. cDNA was purified using Millipore multiwell purification plates and lyophilized PCR products were resuspended in 1x Micro-Spotting Solution Plus (TeleChem) at a concentration of 0.25-0.75 ug ul -1. DNA was spotted onto CMT-GAPS-coated glass slides (Corning) by using Stealth micro-spotting pins (TeleChem).