title: Agilent-013282 Human Genome CGH Microarray 44B (Feature number version)
status: Public on Sep 24 2005
contact.city: Palo Alto
contact.last: Agilent Technologies
contact.email: cag_sales-na@agilent.com
contact.state: CA
contact.country: USA
contact.web_link: http://www.agilent.com
contact.institute: Agilent Technologies
contact.phone: 877-424-4536
organism: Homo sapiens
description: Human Genome CGH Microarray 44B, high-resolution, high performance 60-mer oligonucleotide, allowing genome-wide survey and molecular profiling of DNA copy number changes on a single chip.  It provides comprehensive genome-wide coverage with emphasis on the most commonly studied genomic regions and cancer-related genes.  It consists of ~43,000 60-mer oligonucleotide probes, empirically validated in multiple model systems, spanning coding and noncoding sequences with average spatial resolution of 35kb.  It replaces 44A (G4410A), includes all biological probes from 44A, mapped to the latest NCBI build, 35), plus an additional ~ 2,500 probes to expand coverage in the human genome.  Agilent has taken extra measures to design the microarray that truly represents the coverage and performance needed for this application.  Agilent has developed and optimized assay protocols that enable researchers to use nanogram levels of total genomic DNA without genome complexity reduction.
  
  Arrays of this design have barcodes that begin with 16013282 or 2513282.
  
  Orientation:
  Features are numbered numbered Left-to-Right, Top-to-Bottom as scanned by an Agilent scanner (barcode on the left, DNA on the back surface, scanned through the glass), matching the FeatureNum output from Agilent's Feature Extraction software.
  
  The ID column represents the Agilent Feature Extraction feature number.
  
  Rows and columns are numbered as scanned by an Axon Scanner (barcode on the bottom, DNA on the front surface).
  
  To match data scanned on an Axon scanner, use the RefNumber column contained in the Agilent-provided GAL file as the ID_REF column in sample submissions.
  
  *** A different version of this platform with the Agilent Probe names in the ID column is assigned accession number GPL11068.
manufacture_protocol: see manufacturer's web site at http://www.agilent.com/