title: CSIRO Cane Toad 5.5k cDNA array
status: Public on Nov 27 2008
contact.city: Canberra
contact.first: Andrew
contact.last: Spriggs
contact.email: andrew.spriggs@csiro.au
contact.state: ACT
contact.address:  GPO Box 1600
contact.department: Bioinformatics Group
contact.country: Australia
contact.institute: CSIRO Plant Industry
contact.phone: 612-6246-5193
organism: Rhinella marina
description: 5472 spot cane toad array
manufacture_protocol: A toadlet cDNA library was constructed with the plasmid pCMVSport6 and gene fragments PCR amplified using M13/pUC forward and reverse primers. PCR products from 5000 randomly selected clones were precipitated (sodium acetate/ethanol) and resuspended with ddH2O:DMSO (Dimethyl Sulfoxide, Sigma) in preparation for spotting. Microarray slides were printed using a VersArray ChipWriter Pro microarray robotic system (CSIRO Plant Industry).