title: Tavazoie Lab Escherichia coli 3.2k cDNA array
status: Public on May 13 2008
contact.city: Princeton
contact.first: yirchung
contact.last: Liu
contact.email: yliu@princeton.edu
contact.state: NJ
contact.address: 220 Carl Icahn Laboratory
contact.department: Molecular Biology
contact.country: USA
contact.institute: Princeton University
organism: Escherichia coli K-12
description: The Platform data table reflects a condensed representation of the duplicate-spotted PCR products on the physical array.
manufacture_protocol: We produced in-house DNA microarrays that contain PCR-amplified fragment of DNA derived from predicted open reading frames (ORFs) in the E. coli genome.  All PCR products were analyzed by electrophoresis on 1% agarose gels.  Reactions that resulted in undetectable PCR products were re-amplified using a lower annealing temperature. All PCR products from the final round of amplifications were precipitated with ethanol, then dissolved in 50% DMSO (100-150 μg/ml) and spotted onto Corning GAPS II slides using Affymetrix GMS 417 Arrayer (Santa Clara, CA) in duplicate on each slide. Arrayed slides were baked at 80˚ C for 2 hours and stored in a desiccator at room temperature under vacuum.