title: 081118_Sims_GIS_multi_Burkh_cgh
status: Public on Aug 14 2010
contact.city: SINGAPORE
contact.first: BERNICE
contact.middle: MENGQI
contact.last: SIM
contact.email: SIMB@GIS.A-STAR.EDU.SG
contact.state: SINGAPORE
contact.address: 60 Biopolis Street, Genome; #02-01
contact.department: INFECTIOUS DISEASES 2
contact.country: Singapore
contact.institute: GENOME INSTITUTE OF SINGAPORE
organism: Burkholderiales
description: Using the NimbleGen proprietary photolithography Maskless Array Synthesizer (MAS) technology in situ oligo-synthesis, we fabricated a multi-species pan genome array that has 388943 probes, of which 366064 are data probes, 2074 control probes and 20,000 probes for backgroun estimation and correction. The make-up of these data probes are 55% (of which 60% from Bp K96243) for all sequenced Bp strains, 34% designed for all sequenced Bt strains (of which 90% from Bt E264) and finally 11% designed for Bc strain J2315. The pan genome array has an average melting temperature of 83degC and has a range from 65.4degC to 97.3degC. The average probe length is 50bp, with an average spacing of 60 to 62 bp after removing all repetition and homo-polymer runs.
  native array description file: 081118_Sims_GIS_multi_Burkh_cgh.ndf
  native array description file: 081118_Sims_GIS_multi_Burkh_cgh.pos
manufacture_protocol: High-density DNA array prepared with Maskless Array Synthesizer (MAS) technology. See manufacturer's website at http://www.nimblegen.com/.