title: NCI/ATC Hs-OperonV2
status: Public on Oct 27 2004
contact.city: Gaithersburg
contact.first: David
contact.last: Petersen
contact.email: petersed@mail.nih.gov
contact.state: MD
contact.address: 8717 Grovemont Circle
contact.country: USA
contact.institute: NCI
contact.phone: 301-435-7898
organism: Homo sapiens
description: Attachment: UV cross-linked
  Array description:  The custom arrays are manufactured by the NCI Advanced Technology Center Microarray Facility (NIH/NCI/CCR/ATC).  Microarrays are printed with GeneMachines robotic arrayer from Genomic Solutions (http://www.genomicsolutions.com) that is fitted with a printhead containing 32 SMP3 printing pins from Telechem International (http://www.arrayit.com).
  
manufacture_protocol: The human long oligonuleotide probe set contains 21,329 optimized 70mers (plus controls) obtained from Operon (http://www.operon.com/ ) . The long oligonucleotide probes were purchased in 384-well polypropylene plates (Genetix, 384 micro array) and dissolved in 3XSSC print buffer at a concentration of 20-30 uM.  The microarray spot densities are 160 microns (center-to-center), and one spot is printed per probe per slide.  The microarrays printed on Corning Ultra-GAPS slides (Item #40018) coated with amino-silane and post-processed according to the manufacturer's protocol.