title: Pseudomonas putida KT2440 Mycroarrays
status: Public on Aug 22 2010
contact.city: College Station
contact.first: Yunho
contact.last: Lee
contact.email: yunholee97@gmail.com
contact.state: TX
contact.address: 1501 Harvey Road
contact.department: Chemical engineering
contact.country: USA
contact.institute: Texas A&M
contact.phone: 979-450-3246
organism: Pseudomonas putida
description: The method is described at http://www.mycroarray.com/technology/synthesis.html.
  Probe design software searches for specific probes at the genomic level. The probe candidate is compared to all other expressed sequences from the same organism and the thermodynamic parameters (free energy and melting temperature) are computed for all possible hybridizations between the probe and perfect or non-perfect complementary sequences. If all of these values fall below a predetermined threshold, the probe is considered to be specific to its target. Probes are also selected to be unable to fold into stable secondary structures that may interfere with hybridization. Any probes with low sequence complexity or long stretches of the same base are rejected..
manufacture_protocol: See manufacturer's web site
  (http://www.mycroarray.com/technology/synthesis.html)