title: IMS human 0.6K v.2
status: Public on Dec 22 2008
contact.city: Minato-ku
contact.first: Naoyuki
contact.last: Takahashi
contact.email: takan@ims.u-tokyo.ac.jp
contact.state: Tokyo
contact.address: 4-6-1, Shirokanedai
contact.department: Research Hospital
contact.country: Japan
contact.institute: The Institute of Medical Science, The University of Tokyo
contact.phone: 81-3-5449-5524
organism: Homo sapiens
description: Probes for 207 target genes were spotted singly in the first block, those for 205 target genes in the second block, and those for 203 target genes in the third block.  Probes for 8 positive and 4 negative control genes were spotted singly in every block.  It was confirmed that each spot contained the same amount of probes throughout the three thin chips which were set into a holder together.
manufacture_protocol: In this study, we used a custom-made fibrous oligonucleotide DNA microarray Genopal (Mitsubishi Rayon) for 615 target genes, which were selected from genes encoding cytokines, cytokine receptors, chemokines, chemokine receptors, adhesion molecules, and signal transducers, as well as for 8 positive control genes (GAPDH, ACTB, RPS5, K-ALPHA-1/TUBA1B, ATP5F1, GTF2B, PLA2G5, and TFRC)　and 4 negative control genes (lambda A, lambda B, Omp A1, and Omp A2).
  All oligonucleotide capture probes (70 mer in length), including those for positive and negative controls, were designed to have approximately the same annealing temperature (Tm)　throughout a microarray.  In addition, each probe was designed also to hybridize to its target antisense RNA specifically within 1 kb from 3’-end.
  The custom-made fibrous oligonucleotide DNA microarray, Genopal (Mitsubishi Rayon), was manufactured in the following manner.  Hollow plastic fibers, in which oligonucleotide capture probes were chemically bonded to hydrophilic gel, were bundled in an orderly arrangement, hardened with resin to form a block (12 rows × 19 columns) and then sliced into thin chips.  Finally, three thin chips were set into a holder together.