title: IBBA/CCG_Common bean_90K_v1.0 [condensed]
status: Public on Jul 01 2012
contact.city: Cuernavaca
contact.first: Mario
contact.last: Ramírez
contact.email: mario@ccg.unam.mx
contact.state: Morelos
contact.address: Avenida Universidad S/N
contact.department: Functional Genomics of Eukaryotes
contact.country: Mexico
contact.institute: Centro de Ciencias Genomicas-UNAM
contact.phone: +52 777 3115164
organism: Glycine max
  Phaseolus vulgaris
description: 18,883 specific oligos (35-40mer) were designed on Bean Gene Index Version 3 (Release 3.0. May 22, 2009) tentative consensus (TC) sequences and 11,205 specific oligos (35-40mer) were designed on Soybean UniGene Built #38 by using the program design OligoArray 2.1 (Rouillard et al. 2003). 141 oligonucleotides sequences provided by the manufacturer were used as quality and negative controls. The 90K BeanArrayTM 1.0 was designed with three replicates of each probe randomly distributed across the array to allow measurement of the variability within the array. All the procedures provided were performed as indicated by CombiMatrix protocols available at the CombiMatrix website (www.combimatrix.com).
manufacture_protocol: Probes are directly synthesized on a 90k Combimatrix array by an in situ oligo synthesis system developed by CombiMatrix Corporation (Mukilteo, USA) using the CombiMatrix CustomArray synthesizer. Standard synthesis protocol provided by CombiMatrix was used.