title: Hebrew University Mus musculus SpliceChip (condensed template)
status: Public on Dec 07 2007
contact.city: Jerusalem
contact.first: Ari
contact.last: Meerson
contact.email: arime@cc.huji.ac.il
contact.address: Givat Ram
contact.country: Israel
contact.institute: Hebrew University
organism: Mus musculus
manufacture_protocol: To compose the SpliceChip, C6 amino-modified 70-mer oligonucleotides from the Operon mouse genome oligo set version 3.0 (Operon, Huntsville, AL, USA) were dissolved in spotting buffer [3 × saline sodium citrate (SSC), 1.5 m betain] to a final concentration of 20 mm, and printed on sialyated (SAL-1) Genorama slides (AsperBiotech, Tartu, Estonia) using the MicroGrid spotter (Genomic Solutions, Holliston, MA, USA). The array layout (17.76 × 13.26 mm) contains 12 subgrids, each composed of 12 rows and 12 columns. Tested oligonucleotides were spotted six times each on the array; negative controls were spotted 18 times.