title: mwg-10k-1
status: Public on Mar 14 2007
contact.city: Ciudad de Buenos Aires
contact.first: Mariano
contact.middle: Cesar
contact.last: Salibe
contact.email: msalibe@leloir.org.ar
contact.address: Av. Patricias Argentinas 435
contact.country: Argentina
contact.institute: Gentron LLC
organism: Mus musculus
manufacture_protocol: 50 mer mouse 10K oligo-set (MWG, Germany) were resuspended in 15 ul of DMSO 50% (Stock solution plates). 5 ul from each oligo of the stock solution were transferred to a 384 well plate (Whatman Inc) (Working solution plates). Oligos in the working solution plates were printed onto UltraGap Slides (Corning) using a Versarray chipwriter (Bio-Rad) with Stealth Micro Spotting quill pins (Telechem). Humidity was set at 45%, and temperature was 22-25°C. Pins were loaded in the working plate during 0.5 sec. Distance between spots was 292 um, Dwell time for printing was 0.05 sec, each oligo was spotted one time per slide. After the pins were loaded in the working plate and before printing the slides, a blotting slide was printed 5 times in order to discard the excess of oligo on the pins. After printing on slides, and before loading a new oligo, pins were washed with 3 cycles of 5 sec. wash with water, followed by 1 sec vacuum, the last vacuum time was 10 sec. After printing was completed, oligos were UV crosslinked to the slides on a CL-1000 UV crosslinker (UVP). Printed slides were stored in a dissecator