title: NCI/ATC Hs-OperonV3
status: Public on May 17 2006
contact.city: Gaithersburg
contact.first: David
contact.last: Petersen
contact.email: petersed@mail.nih.gov
contact.state: MD
contact.address: 8717 Grovemont Circle
contact.country: USA
contact.institute: NCI
contact.phone: 301-435-7898
organism: Homo sapiens
manufacture_protocol: Microarrays are printed with GeneMachines robotic arrayer from Genomic Solutions (http://www.genomicsolutions.com) that is fitted with a printhead containing 48 SMP2.5 printing pins from Telechem International (http://www.arrayit.com). The human long oligonuleotide probe set contains 34,960 optimized 70mers (plus controls) obtained from Operon (http://www.operon.com/). The long oligonucleotide probes were purchased in 384-well polypropylene plates (Genetix, 384 microarray) and dissolved print buffer of sodium phosphate (150mM) at ph 8.5 and 4% sodium sulphate at a concentration of 20-30 uM. The microarray spots are printed with 160 micron column x 155 microns row spacing, and one spot is printed per probe per slide. The microarrays were printed on Corning Expoxide slides or Schott Epoxide slides and post-processed according to the manufacturer's protocol.