title: NTUH-K2044 microarray
status: Public on Nov 25 2015
contact.city: Taipei
contact.first: Pei-Fang
contact.last: Hsieh
contact.email: peifanghsieh@yahoo.com.tw
contact.address: 1, Sec 1, Jen-Ai Rd.
contact.department: Department of Microbiology
contact.country: Taiwan
contact.institute: National Taiwan University College of Medicine
organism: Klebsiella pneumoniae
description: spotted single stranded PCR amplicon
manufacture_protocol: The genomic library was constructed from a clinical isolate, NTUH-K2044, obtained from a patient with PLA plus meningitis and endophthalmitis for a KP genome shotgun sequencing project. NTUH-K2044 array contains 2,848 cloned sequences, which covering 99.9% of the entire genome sequence of NTUH-K2044. DNA fragments of K. pneumoniae in pUC18 were amplified by polymerase chain reaction (PCR) using primers to vector sequences and were spotted onto a nylon membrane (Roche) by a computer-controlled XYZ translation system (PM500; Newport).