title: WUSTL Yersinia pestis 8.4k v.1
status: Public on Dec 16 2005
contact.city: St. Louis
contact.first: Seth
contact.middle: Daniel
contact.last: Crosby
contact.email: scrosby@wustl.edu
contact.state: MO
contact.address: 660 S. Euclid
contact.department: Genetics
contact.country: USA
contact.institute: Washington Univeristy School of Medicine
contact.phone: 314-286-1256
organism: Yersinia pestis
description: To construct a gene-specific microarray of the Y. pestis genome, we designed 70mer oligonucleotide array elements. Predicted Y. pestis genes, encoded on both the chromosome and the three plasmids of strain CO92, were obtained from the The Wellcome Trust Sanger Institute (http://www.sanger.ac.uk/Projects/Y_pestis/).  In addition genes derived from human, mouse, rat and virus were included to detect contaminating host mRNA.  Oligonucleotides were designed from these sequences using ArrayOligoSelector.
manufacture_protocol: Oligos were synthesized using standard methods by Illumina (San Diego, CA). These oligonucleotides, as well as Ambion control E. coli sense oligos #7 and #8 (Ambion part# 1781), were dissolved at a concentration of 50 μM in 3X SSC with 0.75M betaine and were printed in duplicate on MWG Epoxy slides by a locally constructed linear servo arrayer (after the DeRisi model, http://derisilabs.ucsf.edu/).