title: Netherlands Cancer Institute human 3.5k BAC array
status: Public on Nov 16 2006
contact.city: Hamburg
contact.first: Simon
contact.middle: A
contact.last: Joosse
contact.email: s.joosse@in-silico.net
contact.address: Martinistrasse 52
contact.department: Institute of Tumor Biology
contact.country: Germany
contact.institute: University Medical Center Hamburg-Eppendorf
organism: Homo sapiens
description: Slides: Code Link Activated Slides (Amersham Biosciences, Prod. No. 300011 00)
manufacture_protocol: Degenerate oligonucleotide PCR-products from all BAC clones were prepared for spotting according to detailed protocols (Pinkel et al, 1998) with some modifications (Alers et al, 1999). All clones (three replicates for each probe) were spotted in randomised fashion across 48 subarrays, each containing 270 spots.