title: IMB Atlantic salmon cDNA microarray
status: Public on Dec 30 2005
contact.city: Halifax
contact.first: Jason
contact.last: Williams
contact.email: jason.williams@nrc-cnrc.gc.ca
contact.state: Nova Scotia
contact.address: 1411 Oxford Street
contact.department: Institute for Marine Biosciences
contact.country: Canada
contact.institute: National Research Council
contact.phone: (902)426-5903
organism: Salmo salar
description: Corning GAPS II
manufacture_protocol: Inserts from 4104 cDNAs were amplified by PCR, purified, resuspended in distilled water and spotted in duplicate on Gap II glass slides in 50% DMSO using a Virtek SDDC-2 ChipWriter at the National Research Council Biotechnology Research Institute. Two-fold dilutions of a plasmid encoding chlorophyll synthetase G4 from Arabidopsis thaliana were included at the bottom of each sub-array to serve as internal controls. The resulting array of 8,208 spots was printed twice  on each slide, giving a total of 16,416 spots.