title: Chulalongkorn Univ Leptospira interrogans spotted 17K oligo array
status: Public on Mar 31 2010
contact.city: Pathumwan
contact.first: Kanitha
contact.last: Patarakul
contact.email: k_patarakul@yahoo.com
contact.state: Bangkok
contact.address: 1873 Rama IV Road
contact.department: Microbiology
contact.country: Thailand
contact.institute: Faculty of Medicine, Chulalongkorn University
contact.phone: 662-256-4132
organism: Leptospira interrogans
manufacture_protocol: 1. Oligos were transferred to 384 well printing plates and resuspended at 250ng/ul in 3xSSC buffer for printing.
  2. The prepared oligos were arrayed onto Schott A+ aminosilane glass slides using the ESI Virtek Arraying Robot. DNA was spotted using Point Technology split pins.
  3. Slides were baked and UV irradiated at 700MJ to crosslink the DNA.