title: Catholic Human 19K
status: Public on Sep 28 2010
contact.city: Suwon, Korea
contact.first: Jung Woo
contact.last: Eun
contact.email: jetaimebin@catholic.ac.kr
contact.state: Korea
contact.address: Department of Gastroenterology
contact.country: South Korea
contact.institute: Ajou University of Korea
organism: Homo sapiens
manufacture_protocol: 1.The 60mers of Human Oligolibrary package representing 18,664 LEADS clusters plus 197 controls (GAPDH) were purchased from Compugen/Sigma-Genosys
  2. Centrifuge all plates at 1000rpm for 3min after vortexing and dissolving
  3. Before printing, add 3X SSX buffer and resuspension probe acting solubilization to each well.
  4. Seal plates with sealing film.
  5. Incubate at 25°C for 3hrs to aid resuspension of DNA.
  6. Shake plates and centrifuge plates at 1000 rpm for 3 minutes.
  7. Centrifuge plates at 2000 rpm for 3 minutes.
  8. Remove seals and cover with plate lids. Place in appropriate location of plate cassette.
  9. After the print run is completed, allow arrayer to print chips
  10. Allow oligo chip to reactive probes, after printing is completed.
  11. Sealing and keeping probes on -20℃ for long time storage
  12. Go post-processing step