title: UNIS_S COELICOLOR_SCo3_8149
status: Public on Apr 30 2008
contact.city: Manchester
contact.first: Giles
contact.last: Velarde
contact.address: MIB
contact.country: United Kingdom
contact.institute: University of Manchester
organism: Streptomyces coelicolor
manufacture_protocol: 1. Arrays are printed in 12X4 blocks using a Genetix QArray2 high-throughput spotting robot with tungsten split pins at room temperature, 45% Humidity and a spotting buffer containg 150mM PO4/0.005% SDS/Tween20.
  2. After printing, the arrays stay on the platform in the printing chamber overnight (printing starts in early afternoon).
  3. Next day the oligo arrays are cross linked at 600 mJ/cm2.