title: UMD Metarhizium anisopliae V1.0
status: Public on Jun 25 2009
contact.city: College Park
contact.first: Sibao 
contact.last: Wang
contact.email: swang1@umd.edu
contact.state: MD
contact.address: Baltimore Avenue
contact.department: Entomology
contact.country: USA
contact.institute: University of Maryland
contact.phone: 301-405-1603
organism: Metarhizium anisopliae
manufacture_protocol: The construction of the cDNA microarrays used in this study has been previously described (Wang et al., 2005). This array harbors PCR amplified fragments from the unique cDNA clones from M. anisopliae var. anisopliae ARSEF 2575 and a few genes from M. anisopliae var. acridum ARSEF 324 absent from the libraries of ARSEF 2575. In total, 1730 amplified clones were printed in triplicates on the slides. Additional background control was provided by 30 randomly distributed spots of 3×SSC buffer. Printing, hybridization, and scanning of slides was as described before (Freimoser et al., 2005).