title: MPIIB MWG-Ocimum Biosolutions E.coli K12 array
status: Public on May 14 2018
contact.city: Berlin
contact.first: Hans-Joachim
contact.last: Mollenkopf
contact.email: mollenkopf@mpiib-berlin.mpg.de
contact.address: Charitéplatz 1
contact.country: Germany
contact.institute: Max-Planck-Institute for Infection Biology
contact.phone: +49 30 28460 482
organism: Escherichia coli K-12
description: total 9216 features (4608 features spotted in duplicate)
manufacture_protocol: The ssDNA probes were spotted in Nexterion spot2 buffer (Schott AG, Mainz, Germany) with a BioRobotics MicroGrid II robot printer (Genomic Solutions Ltd., Huntingdon, UK) onto glass slides coated with poly-L-lysine at 50-55% humidity and room temperature using MicroSpot 2500 quill pins (Genomic Solutions Ltd.) in 2 identical replicates. Oligonucleotides were crosslinked to the support by UV irradiation with a total energy of 240mJ UV and blocked with succinic anhydride and 1-methyl-2-pyrrolidone in borate buffer (Fluka, Deisendorf). Please note that feature number 17, 306, 595, 884, 1173, 1462, 1751, 2040, 2329, 2618, 2907, 3196, 3485, 3774, 4063, 4352, 4641, 4930, 5219, 5508, 5797, 6086, 6375, 6664, 6953, 7242, 7531, 7820, 8109, 8398, 8687, 8976 are empty, derived from the combination of MicroGrid II printing and Agilent Technologies Microarray scanner BA.