title: Transcriptome of Lilium longiflorum generative cells
status: Public on Oct 24 2007
contact.city: Parkville
contact.first: Takashi
contact.last: Okada
contact.email: takashi.okada@csiro.au
contact.state: VIC
contact.address: Cnr of Royal pde and Tin alley
contact.department: Faculty of Land and Food Resources
contact.country: Australia
contact.institute: The University of Melbourne
contact.phone: +61-3-8344-5051
organism: Lilium longiflorum
manufacture_protocol: Insert of 1234 cDNA clones and 3 marker genes were amplified with an M13 universal primer set.  PCR products were purified and resuspended in 50% DMSO solution.  Array was printed on Corning Gaps II slides (Corning Incorporated Life Sciences, MA, U.S.A.) using a 4 x 4 array of 16 telecam pins 21 dots x 21 dots per grid each dot approximately 100 microns apart.  Each cDNA was spotted in triplicate.  A set of universal control including human and mouse genes was also spiked.  Post printing cross-linking consisted of UV and slide baking for 2 hours at 80oC.