title: OSU Upland cotton line Im216 SSH library array
status: Public on Jan 01 2006
contact.city: Stillwater
contact.first: Margaret
contact.last: Essenberg
contact.email: margess@biochem.okstate.edu
contact.state: OK
contact.address: 246 NRC, OSU
contact.department: Biochemistry & Mol. Biol.
contact.country: USA
contact.institute: Oklahoma State University
organism: Gossypium hirsutum
description: Production of spotted microarrays of PCR products from cotton cDNAs by OSU:  PCR products were from an SSH library enriched in transcripts up-regulated at 8, 14, 20, 30, 45, or 60 hpi in Im216 leaves following inoculation with Xanthomonas campestris pv. malvacearum relative to non-inoculated control tissue.  Using an OmniGrid 100 arrayer (GeneMachines) fitted with 2x2 Telechem SMP4 quill pins, 191 PCR products from the library (116 non-redundant) plus ones from 14 donated cotton cDNAs in 3xSSC at about 360 ng/µl were spotted onto GAPS II glass slides (Corning Life Sciences) at 23-26°C and 55% RH.  Spots were arranged 10x10 per block (48 total blocks), with spot centers 350 µm apart.  The collection was arrayed 12 times per slide, as two columns of six arrays.  Slides were left to dry overnight on the arrayer, rehydrated for 30sec, and snap-dried.  Probes were immobilized by baking at 85°C overnight, and the slides were stored in slide holders in a desiccator at room temperature.
manufacture_protocol: OminiGrid 100 fitted with Telechem SMP4 quill-style pins.  Printed at room temperature (23-26C) at 55% RH.