title: SIPPE Brassica napus 8.1K cDNA Microarray (v1.0)
status: Public on Feb 26 2008
contact.city: Shanghai
contact.first: Hong-Wei
contact.last: Xue
contact.email: gzwu@sibs.ac.cn
contact.state: Shanghai
contact.address: 300 Fenglin Road
contact.department: molecular genetics
contact.country: China
contact.web_link: http://www.plantsignal.cn
contact.institute: Institute of Plant Physiology & Ecology
organism: Brassica napus
description: The genechip is a glass-based cDNA chip. The chip has 8192 spots, including 8095 Brassica Napus genes, 81 blanks and 16 negative controls. The chip is divided into 32 sub-matrix. There is 16*16 spots in every sub-matrix. The distance between two spots is 0.281 millimeter.
manufacture_protocol: 1、Amplify the insertions (in pBSF vector) by PCR using T3 and T7 primers;
  2、Check the quality of the PCR products through electrophoresis;
  3、Purify the products using isopropyl alcohol;
  4、Dissolve the PCR products(in 96-well plates) in spotting buffer;
  5、Transfer the products into 384-well plates;
  6、Spotting the genechip using OmniGrid Arraying System with MicroQuill 2000 Pins;
  7、Hydrate the chip for 30 min in Arraying System, dry the chip for 2 hour at room temperature;
  8、Block the chip for 15 min with blocking buffer, wash it with water, dry it at room temperature.