title: MGAC_Ecoli_v1.0
status: Public on Sep 28 2009
contact.city: Seoul
contact.first: Sung Ho
contact.last: Yoon
contact.address: 120 Neungdong-ro, Gwangjin-gu
contact.department: Department of Bioscience and Biotechnology
contact.country: South Korea
contact.institute: Konkuk University
organism: Escherichia coli
manufacture_protocol: Oligonucleotide microarrays were fabricated by which both E. coli B REL606 and K-12 MG1655 could be tested for the synthesis of both common and unique mRNAs. Probes (70-mers) were designed by Array Designer (Premier Biosoft International) and cross-hybridization was checked by BLASTN. A total of 4,819 probes were synthesized by Illumina. The probes represented 3,762 genes that shared over 80-mer of sequence in the two E. coli strains, 382 genes found uniquely in REL606, and 534 genes unique to MG1655. The other probes were positive and negative controls. The probes were spotted in duplicate onto a GAPSII glass slide (Corning Inc.) by Digital Genomics in Seoul, Korea.