title: ULB-Erasme_MGE_Staphaureus_v2.00
status: Public on Feb 15 2009
contact.city: Brussels
contact.first: Ricardo
contact.middle: Luis
contact.last: De Mendonca
contact.email: ricardo.de.mendonca@ulb.ac.be
contact.address: Route de Lennik 808
contact.department: Microbiology
contact.country: Belgium
contact.institute: ULB - Hôpital Erasme
contact.phone: +32-2-555 8903
organism: Staphylococcus aureus
description: 1326 probes recognize 403 target genes located on the accessory or core variable genome. Target genes encode antimicrobial resistance factors (35 %), virulence factors (28 %) and adhesins (31%).Controls:A total of  102 controls are included in the set. This encompasses:Negative controls:  84 buffer only and 6 probes corresponding to human actin and gapdh genes were positioned throughout the array to assist in determining background. Each human negative control was spotted in triplicatePositive controls:  12 probes corresponding to two distinct 23S rRNA genes and two distinct 16S rRNA genes. Each control was spotted in triplicate.
manufacture_protocol: Custom made commercial DNA-microarray selectively target 403 staphylococci genes. 60-mer oligonucleotides were designed to specifically target those genes by using the Array Designer 4.0 software (Eurogentec, Liège, Belgium - www.eurogentec.com/product/research-oligonucleotide-design.html). A local BLAST database containing the eight complete S. aureus genomes was used to ensure probe specificity. Oligonucleotide probes were synthesized (http://www.eurogentec.com/product/research-oligonucleotide-synthesis-and-quality-control.html), then printed in triplicate on UltraGaps glass slides (Corning) with the VersArray® ChipWriter Pro System (Bio-Rad)