title: DGRC-D. melanogaster-DGRC1-15552-v2
status: Public on Jun 02 2006
contact.city: Bloomington
contact.last: Drosophila Genomics Resource Center (DGRC)
contact.email: dgrc@cgb.indiana.edu
contact.state: IN
contact.address: 1001 E. 3rd St.
contact.department: Indiana University
contact.country: USA
contact.web_link: http://dgrc.cgb.indiana.edu/
contact.institute: Drosophila Genomics Resource Center (DGRC)
contact.phone: 812-855-5510
organism: Drosophila melanogaster
description: DGRC-1 amplicon transcriptome microarrays are spotted with DNA fragments amplified from genomic DNA of the Oregon R strain of Drosophila melanogaster using gene-specific oligonucleotide primer pairs made by Incyte Genomics.  The primers were designed against release 1 of the annotated genome sequence and were selected to amplify fragments which have minimal sequence similarity to other genes, size range between 100-600 bp, and location biased towards the 3'-end of transcripts. The array also contains the following controls: a ubiquitously expressed gene (actin 5C), a spotting buffer-only control (3XSSC plus 1.5M Betaine), exogenous(non-Drosophila) probes (7 from Arabidopsis and 8 Ambion array control PCR spots from bacteria that can be used with spiking RNAs), and three exogenous markers: GAL4, lacZ, and FLP.  The DGRC arrays are printed using an Omnigrid 300 contact-printing instrument and 48 silicon split-pins on Corning GAPS II slides.
manufacture_protocol: http://dgrc.cgb.indiana.edu/microarrays/support/protocols.html