title: Breakthrough Human 32K v5
status: Public on Aug 30 2014
contact.city: London
contact.first: Richard
contact.middle: Dafydd
contact.last: Williams
contact.email: richard.williams@ucl.ac.uk
contact.address: 30 Guilford Street
contact.department: Developmental Biology and Cancer
contact.country: United Kingdom
contact.institute: UCL Institute of Child Health
organism: Homo sapiens
manufacture_protocol: DNA from the 32K human BAC re-array set, a tiling path collection of BACs covering the entire human genome, was obtained from Pieter de Jong at the Children's Hospital Oakland Research Institute (CHORI). DNA was amplified by Genomiphi version 1 according to the manufacturer’s instructions. Following amplification, the DNA collection was randomized for spotting. DNA was mixed with an equal volume of 2x Microarray Spotting Solution (Genetix) and printed on UltraGaps APS coated microarray slides (Corning) using a Genetix QArray 2 spotter. The slides were printed with 48 blocks per array (4 columns, 12 rows) with a layout of 714 features per block (14 columns, 51 rows orange-pack pattern), a total of 34272 features with a nominal size of 110-120 micrometres. After printing, DNA was fixed to the slide using UV cross-linking using 200mJ with a Stratalinker (Stratagene). Slides were then stored in a desiccating cabinet before use.