title: WUSTL Caenorhabditis elegans Whole Genome 23k Oligo Array
status: Public on Sep 21 2007
contact.city: Boulder
contact.first: liang
contact.last: zhang
contact.email: liangz@colorado.edu
contact.state: CO
contact.address: University of Colorado at Boulder, Han lab, Department of MCD Biology, Campus box 0347
contact.country: USA
contact.institute: university of colorado at boulder
organism: Caenorhabditis elegans
manufacture_protocol: General Information
  Arrays printed on Corning Epoxy slides
  Slides have been post-processed and are ready to hyb.
  Common spot diameter – 90-110μ
  Spot to spot distance – 200μ
  • 22614 features
  • 22490 oligo features
  • 124 control features
  • 12 Rows
  • 4 Colums
  • 48 Subarrays
  Feature Concentration Information
  A. thaliana Features printed at 50 uM
  Controls (Positive and Negative) printed at 40 uM
  Poly(dA)40–60 oligonucleotide printed at 0.01 μg/μL
  Salmon sperm DNA printed at 0.1 μg/μL
  Human COT-1 DNA® printed at 0.1 μg/μL
  Control Feature Information
  Control Oligonucleotides
  Number Arabidopsis thaliana Gene Identification
  Positive Controls (70-mer)
  10 Oligonucleotide 1 (Cab) A. thaliana photosystem I chlorophyll a/b-binding
  protein
  (GenBank® database accession # X56062)
  10 Oligonucleotide 2 (RCA) A. thaliana RUBISCO activase
  (GenBank database accession #X14212)
  10 Oligonucleotide 3 (rbcL) A. thaliana ribulose-1,5-bisphosphate
  carboxylase/oxygenase large subunit
  (GenBank database accession#U91966)
  10 Oligonucleotide 4 (LTP4) A. thaliana lipid transfer protein 4
  (GenBank database accession #AF159801)
  10 Oligonucleotide 5 (LTP6) A. thaliana lipid transfer protein 6
  (GenBank database accession #AF159803)
  10 Oligonucleotide 6 (XCP2) A. thaliana papain-type cysteine endopeptidase
  (GenBank database accession #AF191028)
  10 Oligonucleotide 7 (RCP1) A. thaliana root cap 1
  (GenBank database accession #AF168390)
  10 Oligonucleotide 8 (NAC1) A. thaliana NAC1
  (GenBank database accession #AF198054)
  10 Oligonucleotide 9 (TIM) A. thaliana triosphosphate isomerase
  (GenBank database accession #AF247559)
  10 Oligonucleotide 10 (PRKase) A. thaliana PRKase gene for ribulose-5-phosphate
  kinase
  (GenBank database accession #X58149)
  Negative Controls
  10 E. coli – B1444 oligonucleotide
  8 E. coli – rRNA oligonucleotide
  2 Poly(dA)40–60 oligonucleotide
  2 Salmon sperm DNA
  1 Human COT-1 DNA®
  1 3X SSC/0.75 M Betaine
  124 – Total Control Features