title: CGP Atlantic cod 20K oligo array v1.0
status: Public on Nov 05 2010
contact.city: St. John's
contact.first: Marije
contact.last: Booman
contact.email: mbooman@mun.ca
contact.state: NL
contact.address: 1 Marine Lab Road
contact.department: Ocean Sciences
contact.country: Canada
contact.institute: Memorial University of Newfoundland
organism: Gadus morhua
manufacture_protocol: Array design: The array contains 20,000 oligos, each spotted in duplicate, representing unique contigs selected from the EST database which was established by the Atlantic Cod Genomics and Broodstock Development Project (CGP), accessible on request through www.codgene.ca. Also included are 1472 control features (cod actin, human actin, SpotReport Alien Oligos 4, 6, 7 an 8 (Stratagene) ands buffer-only spots), resulting in a total of 41,472 features on the array. All probes are in sense orientation.
  Array Printing: Oligonucleotides were provided by IDT in Genetix X7022 384-well plates, each well containing 1 nmole of 50-mer oligo (dried). Oligos were resuspended in 30 μl of Schott Nexterion Spot printing buffer to a final oligo concentration of 30 μM. Oligos were spotted in duplicate on epoxy-coated glass slides (Schott Nexterion E slides) using a Genomic Solutions OmniGrid 100 microarray printer equipped with silicon print pins (Parallel Synthesis Technology SMT-S50). The array contains 41,472 features spotted on 48 blocks of 29 rows and 30 columns each (the last row of each block contains only 24 features).