title: Arizona Gallus gallus 20.7K Oligo Array v1.5
status: Public on Feb 20 2011
contact.city: Tucson
contact.first: Parker
contact.middle: B
contact.last: Antin
contact.email: pba@email.arizona.edu
contact.state: AZ
contact.address: 1656 E. Mabel Street
contact.department: Cell Biology and Anatomy
contact.country: USA
contact.institute: University of Arizona
contact.phone: 52062606382
organism: Gallus gallus
manufacture_protocol: 1. Oligo Design.  The chicken oligos were designed by Carre et al. (2007) using gene predictions from Ensembl, TIGR, full-length cDNAs from the BBSRC UMIST ChickEST database, and chicken bursal EST sequences from Jean-Marie Buerstedde's DT40 database. Sequences from these resources were aligned and 70mer oligos designed against regions common to more than one aligned sequence. Oligos were then designed using OligoArray2.0.  2. Oligo Array Manufacturer.  The chicken oligo set [Gallus gallus (chicken) Roslin/ARK CoRe Array V1.0] was manufactured by Operon Technologies, Inc.. The chicken oligo set (Catalog # 851102) contains 20,460 long oligo probes (300 pmol) arrayed in 54 384-well plates. Four wells in each of the 54 384-well plates contained the Operon Production Tracking Oligo opHsV04NC000001, which is a randomly-generated oligo sequence with a length of 30 bases.  3. Printing.  This 20.7K chicken oligo array contains 20,676 features that include 20,460 70mer oligos and 216 control spots of the Operon Production Tracking Oligo opHsV04NC000001. The oligos (300pmol) were dissolved in 8 microliters of 50% DMSO for printing. The chicken oligo array was printed on Corning® UltraGAPSTM aminosilane coated slides (Cat # 40018) using a Virtek ChipWriter Pro (Model # HCO1-M) robot fitted with 48 TeleChem (SMP2.5) pins. The slides were printed in a HEPA-filtered positive-pressure chamber maintained at 24 degrees C and 48% relative humidity (RH)(± 2 %RH). The printed slides were dried overnight at room temperature (22-25 degrees C with 20-40 %RH). The slides were then stored in a chemical (Drierite) desiccator at room temperature until use.