title: IFC UNAM Mouse 22K v1
status: Public on Aug 22 2024
contact.city: Guadalajara
contact.first: Ana
contact.middle: Soledad
contact.last: Sandoval-Rodriguez
contact.email: anasol44@gmail.com
contact.state: Jalisco
contact.address: Sierra Mojada 950
contact.department: Molecular Biology and Genomics
contact.country: Mexico
contact.institute: University of Guadalajara
contact.phone: +52-3339526314
organism: Mus musculus
manufacture_protocol: 1. The Mouse 65-mer oligo library from Sigma-Genosys oligo sets was used. It contains 22,000 gene specific oligonucleotide probes representing the 70% of mouse genome.
  2. Oligo library was resuspended to 50µM in Micro Spotting solution (ArrayIt Brand Products.).
  3. SuperAmine coated slides 25x75 mm (TeleChem International INC) were printed using SDDC robot (Virtek) with 48 pins (Y= 12 meta rows; X= 4 meta columns; Y1= 22 rows; X1= 22 columns; with a dot spacing of Y1= 190 micron and X1= 190 microns). Fixed at 80°C for 4 hours.
  4. For pre-hybridization the slides were re-hydrated with water vapor at 60°C, and fixed with two cycles of UV light (1200J). After boiling for two minutes at 92°C, slides were washed with 95% ethanol for one minute and prehybridzed in 5X SSC, 0.1% SDS and 1% BSA for one hour at 42°C.