title: Custom Vibrio fischeri GeneChip Array (Affymetrix)
status: Public on Jun 01 2007
contact.city: Vancouver
contact.first: L. Caetano
contact.middle: M.
contact.last: Antunes
contact.email: antunes@mail.ubc.ca
contact.state: BC
contact.address: #367 - 2185 East Mall
contact.department: Michael Smith Laboratories
contact.country: Canada
contact.institute: The University of British Columbia
contact.phone: 604-827-3921
organism: Aliivibrio fischeri
manufacture_protocol: Custom GeneChips (11 uM feature size) representing >99% of the annotated open reading frames in the V. fischeri ES114 genome, as well as 125 intergenic regions greater than 150 bp, were constructed by Affymetrix Inc. (Santa Clara, CA). GeneChips were designed as described by the manufacturer in their GeneChip Custom Express Array Design Guide (www.affymetrix.com) except that, due to the low GC content of V. fischeri DNA, 16 probe pair sets (25-bp oligonucleotide probe length) were used per gene sequence. Poly-A control sequences of dap, lys, phe, and trp from Bacillus subtilis were also included.