title: MMGG Pig X-tiling path 785 BACs v1
status: Public on May 30 2012
contact.city: Cambridge
contact.first: Ben
contact.last: Skinner
contact.email: bms41@cam.ac.uk
contact.address: Tennis Court Road
contact.department: Department of Pathology
contact.country: United Kingdom
contact.institute: University of Cambridge
organism: Sus scrofa
manufacture_protocol: Microarray construction followed the protocol of Fiegler et al (2007). A pig X-chromosome tiling path was provided by the Wellcome Trust Sanger Institute. Briefly, the BAC clones of the tiling path were grown overnight from glycerol stocks in LB in 96 well deep-well plates. BAC DNA was extracted by mini-prep, purified via 96-well filter-plates and eluted in ddH2O. The purified products were amplified by DOP-PCR in three separate reactions using three different DOP primers. Amplified DOP-PCR products were tagged with an aminolinker by PCR, filter-purified and eluted in spotting buffer. Amino-linked products were spotted onto CodeLink CodeLink activated slides (GE Healthcare UK Limited) using an MGII610 (BioRobotics).
  1. Fiegler H, Redon R, Carter NP: Construction and use of spotted large-insert clone DNA microarrays for the detection of genomic copy number changes. Nat. Protocols 2007, 2:577–587.