title: JCVI Aspergillus flavus NRRL3357 27.6k oligo array
status: Public on Aug 05 2008
contact.city: Rockville
contact.first: Natalie
contact.last: Fedorova
contact.email: natalief@jcvi.org
contact.state: MD
contact.address: 9704 Medical Center Dr
contact.department: Infectious Disease
contact.country: USA
contact.institute: JCVI
contact.phone: 301-795-7756
organism: Aspergillus flavus NRRL3357
  Aspergillus oryzae RIB40
description: At least one oligo was designed from each gene model larger then 100 amino acids.  For genes with more than one exon, oligos were designed based on the second exon sequence.  For genes bigger than 4 kb, two oligos were designed from 5’ and 3’ ends.
manufacture_protocol: The 70-mer oligo microarray was designed based on CDS annotated from the A. flavus NRRL 3357 whole genome sequence.  A total of 12,160 oligos were synthesized by Invitrogen.  They represent 12,125 sequences, including 11,837 A. flavus genes, 278 A. oryzae–specific genes (not present in A. flavus), and 10 corn genes that show resistance to A. flavus.  The oligos were printed in duplicate on Corning glassUltraGAPs amino-silane coated slides at JCVI using a high density automated arrayer (Intelligent Automation, Cambridge, MA 02139).