title: Plants 4K miRNA LC_MRA-1038_miRNAPlants_10.0 + Bn_PsRNAs
status: Public on Dec 15 2009
contact.city: Madrid
contact.first: Anja
contact.last: Buhtz
contact.email: anja.buhtz@upm.es
contact.address: Campus de Montegancedo, M40,km38
contact.country: Spain
contact.institute: Centro de Biotecnologia y Genomica de Plantas
organism: Physcomitrium patens
  Pinus taeda
  Populus trichocarpa
  Arabidopsis thaliana
  Brassica napus
  Glycine max
  Medicago truncatula
  Oryza sativa
  Saccharum officinarum
  Sorghum bicolor
  Triticum aestivum
  Zea mays
  Selaginella moellendorffii
description: Plant miRNA microarray with 100% coverage of mature miRNAs listed in miRBase version 10.0 and additional custom small RNA sequences from Brassica napus phloem sap (Bn_PsRNAs)
manufacture_protocol: Arrays are made based µParaflo microfluidic technology (Atactic Technologies). On the µParaflo microfluidic chip, each detection probe consists of a chemically modified nucleotide coding segment complementary to target microRNA (from miRBase, http://microrna.sanger.ac.uk/sequences/) or other RNA (control or customer defined sequences) and a spacer segment of polyethylene glycol to extend the coding segment away from the substrate. The detection probes are made by in situ synthesis using PGR (photogenerated reagent) chemistry.