title: LC Sciences Branchiostoma belcheri Custom Array LCB_D140317
status: Public on Dec 08 2016
contact.city: Beihai
contact.first: Xin
contact.last: Liao
contact.email: njuliaoxin@163.com
contact.state: Guangxi
contact.address: 2# JinHaian Road
contact.department: School of Life Sciences
contact.country: China
contact.institute: Nanjing University
contact.phone: +8618677911616
organism: Branchiostoma belcheri
manufacture_protocol: Arrays are made based µParaflo microfluidic technology (Atactic Technologies). On the µParaflo microfluidic chip, each detection probe consists of a chemically modified nucleotide coding segment complementary to target microRNA (from miRBase, http://microrna.sanger.ac.uk/sequences/) or other RNA (control or customer defined sequences) and a spacer segment of polyethylene glycol to extend the coding segment away from the substrate. The detection probes are made by in situ synthesis using PGR (photogenerated reagent) chemistry.