title: CGH X 1K-2
status: Public on Sep 25 2007
contact.city: Leuven
contact.first: Rekin's
contact.last: Janky
contact.email: Nucleomics.Bioinformatics@vib.be
contact.address: Herestraat 49 Box 816
contact.department: Nucleomics Core
contact.country: Belgium
contact.institute: VIB
organism: Homo sapiens
description: In total the array design contains 8096 features printed in a layout of 4 meta rows, 4 meta columns,  18 rows per block and 16 columns per block.  The distinct BAC and PAC sequences were printed in 2 replicates. This grid is printed twice on the glass slides allowing for two microarray arrays per slide.
  The Platform data table reflects a condensed representation of the array's replicate features.  The full array layout representing all of the individual features is linked as a supplementary file at the foot of this record.
manufacture_protocol: MicroGrid Printing ProtocolEquipment:
  1. Microgrid (Genomics Solutions, UK).
  2. 384 microtiter plates (X7022, Genetix)
  3.  4x4 MicroSpot quill pins - 10K (cat# BR001489, Genomics Solutions, UK)
  4. Codelink slides (cat#: 30001100, GE Healthcare).
  Printing 2 arrays of 4x4 blocks on 1 microscope slide:
  1. Dissolve PCR products in 250mM Phosphate buffer, 0.00025% sarcosyl at concentrations of 300 ng/ul.
  2. Set spotter settings for 2 arrays of 4x4 blocks printed with 16 pins: column pitch = 200 um, row pitch = 200 um; Humidity 55%; 101 spots per source visit.
  3. Make sure room temperature is between 20-24 degrees Celsius.
  4. Place microtiter plates in the plate hotel with A1 in front left corner.
  5. Place 50 slides on the slide-deck.
  6. Start spotting early in the morning and stop spotting procedure at end of the day and remove pin set. Leave spotter overnight with vacuum and humidity settings on.
  7. Incubate the slides in humid chamber with saturated NaCl for 24 to 72 hours.
  8. Block 1%NH4OH.
  9. Slides can be stored for at least 6 months in a dry-cabinet.