title: L.interrogans PCR-based array version 1
status: Public on Mar 12 2008
contact.city: shanghai
contact.first: xiaokui
contact.last: guo
contact.email: microbiology@sjtu.edu.cn
contact.state: shanghai
contact.address: ChongQing nan Road 280
contact.department: Department of Microbiology and Parasitology
contact.country: China
contact.institute: Shanghai Jiao Tong University
contact.phone: 86-021-64453285
organism: Leptospira interrogans
description: The genechip is a glass-based DNA chip. The chip has 11664 spots, including 3290 L.interrogans genes, 19 positive  and 10 negative controls. The chip is divided into 36 sub-matrix. There is 18*18 spots in every sub-matrix.
manufacture_protocol: 1.PCR-amplified 3290 CDSs (accession number GB: AE010300 for CI and GB: AE010301 for CII) of L.interrogans 56601 and purified in 96-wells plates.
  2.PCR products are arrayed in 384-well flat-bottom plates. Each well contains 10 μl(about 2μg)  PCR products  finally purified and dissolved in 50% DMSO in TE at concentrations of 200ng/ul.
  3.Centrifuge plates at 2000 rpm for 3 minutes.
  4.Spotting the genechip using OmniGrid Arraying System robotically. Each products were arrayed in triplicate.
  5.Hydrate the chip for 30 min in Arraying System, dry the chip for 2 hour at room temperature
  Block the chio for 15min with blocking buffer, wash it with water, dry it at room temperature.