title: Gallus gallus 14K A-MEXP-831
status: Public on May 07 2014
contact.city: Cambridge
contact.first: Antony
contact.last: Jackson
contact.email: apj10@cam.ac.uk
contact.state: Cambridgeshire
contact.address: Tennis Court Road, Cambridge
contact.department: Department of Biochemistry
contact.country: United Kingdom
contact.institute: University of Cambridge
organism: Gallus gallus
manufacture_protocol: Clones for the target sequences were arrayed and grown overnight in Ampicillin Lbroth in 1 ml deep 96 well plates
  Plasmids were prepared from the clones using the Qiagen plasmid prep kit on a Bio8000 Robot
  The purified plasmids were used as PCR templates using T7 and T3 primer sequences to amplify the multiple cloning blocks with the inserts.
  The amplified DNA was purified on Millipore Multiscreen plates.
  The DNA was quantified by picogren and diluted to a concentrtaion of 250ng/ul DNA in 1 x spotting buffer
  The DNA was printed on to Corning CMT-GAPS slides using the BioRobot MicroGrid II
  Slides were allowed to Air dry prior to processing according to manufacturers protcol for blocking any unused binding sites.
  Slides were then stored desicated until used