title: Candida albicans ENS oligo array version 1.0
status: Public on Nov 17 2010
contact.city: New Delhi
contact.first: Rajendra
contact.last: Prasad
contact.email: rp47jnu@gmail.com
contact.state: Delhi
contact.address: New Mehrauli Road
contact.department: School of Life Sciences
contact.country: India
contact.institute: Jawaharlal Nehru University
contact.phone: 01126704509
organism: Candida albicans
manufacture_protocol: 1.Oligos are arrayed in Greiner 384-well flat-bottom plates. Each well contains 600 pmol of 70-mer oligo.
  2. Resuspend oligos in Corning buffer to 20 uM and rearray 10 µL into 384-well, Genetix polystyrene V-bottom plates (cat# X6004).
  3. Shake plates near maximum rotational speed on flat-bed shaker for 1 minute.
  4. Centrifuge plates at 2000 rpm for 3 minutes.
  5. Print oligonucleotides in duplicate on corning ultragaps II slides, using a Biorobotics spotting device and 16 pins.
  6. Fix DNA on slides using UV treatment (1200 mJ default set up in a stratalinker device)