title: MIT-HRH-Celegans-MAS8
status: Public on Jan 06 2010
contact.city: Cambridge
contact.first: Ezequiel
contact.last: Alvarez-Saavedra
contact.state: MA
contact.address: 77 Massachusetts Avenue  Room 68-411
contact.department: Biology
contact.country: USA
contact.institute: MIT
organism: Caenorhabditis elegans
manufacture_protocol: Microarray probes were oligonucleotides with sequences complementary to microRNAs and other small RNAs. Each probe was modified with a free amino group linked to its 5' terminus through a 6-carbon spacer (IDT) and was printed onto amine-binding slides (CodeLink, Amersham Biosciences). Printing and hybridization were done using the protocols from the slide manufacturer with the following modifications: the oligonucleotide concentration for printing was 20 μM in 150 mM sodium phosphate pH 8.5, and hybridization was at 50°C for 6 h.