title: Rana pirica tadpole-stage cDNA 3k array
status: Public on Jun 15 2009
contact.city: Fujisawa
contact.first: Tsukasa
contact.last: Mori
contact.email: mori.tsukasa@nihon-u.ac.jp
contact.state: kanagawa
contact.address: Kameino 1866
contact.department: Nihon University College of Bioresource Sciences
contact.country: Japan
contact.institute: Nihon University
contact.phone:  01-(81)-466-84-3682
organism: Rana pirica
description: Tadpole Rana pirica cDNA microarray
  Keywords = Tadpole Rana pirica
  Keywords = cDNA array
  Keywords = gene expression
manufacture_protocol: Inserts in the pCRII-TOPO vector (Invitogen) from the subtraction experiments were amplified with PCR using primers from sequences flanking the cloning site.  The PCR products (1020 clones) were visualized on 0.8% agarose gel to ensure adequate PCR amplification prior to being robotically printed onto glass slides (DNA Chip Research Inc).  After electrophoresis, PCR products were performed isopropanol precipitation without salt and glycogen.  These PCR products were spotted in triplicate onto the glass slide.