title: WUSTL Brugia malayi v.1
status: Public on Dec 06 2004
contact.city: St. Louis
contact.first: Seth
contact.middle: Daniel
contact.last: Crosby
contact.email: scrosby@wustl.edu
contact.state: MO
contact.address: 660 S. Euclid
contact.department: Genetics
contact.country: USA
contact.institute: Washington Univeristy School of Medicine
contact.phone: 314-286-1256
organism: Brugia malayi
description: B. malayi clusters for arrays were selected from 8392 clusters generated and posted at the website http://nema.cap.ed.ac.uk/nematodeESTs/nembase
  These clusters were derived from > 20 cDNA libraries that represent the major B. malayi life cycle stages.  They represent approximately ~40% of the total number of predicted genes for B. malayi.
  Clusters with multiple ESTs, predicted homology by BLAST, and sequence permitting design of a unique 65-mer oligonucleotides were chosen for inclusion on the array.  Oligos were synthesized from the consensus sequence of selected clusters (n=3569) by standard methods by Illumina (San Diego, CA).   The oligonucleotides (50nM in 3x SSC with 0.75M betaine) were printed in duplicate on MWG Epoxy slides (MWG Biotech Inc, High point, NC) by a locally constructed linear servo arrayer (after the DeRisi model, http://derisilabs.ucsf.edu/ ).
  Keywords = brugia malayi nematode worm parasite filaria filariasis