title: Tsinghua University mammalian 2K microRNA microarray
status: Public on Feb 02 2007
contact.city: Beijing
contact.first: yong
contact.last: guo
contact.email: yongguo@tsinghua.edu.cn
contact.state: Beijing
contact.address: Qinghuayuan
contact.department: Medical Systems Biology Research Center
contact.country: China
contact.institute: Tsinghua University
contact.phone: (86)-10-62796071
organism: Homo sapiens
  Mus musculus
  Rattus norvegicus
description: Altogether 509 mature miRNA sequences were assembled and integrated into our miRNA microarray design, these comprised 435 human (including a further 122 predicted miRNA sequences from published references (Xie et al., Nature 2005,434:338-345 ), 196 rat and 261 mouse mature miRNAs from the miRNA Registry (Sanger, accessed Sept. 2005).  In addition, we designed 8 short oligonucleotides that possessed no homology to any known RNA sequence and we produced their corresponding synthetic miRNAs by in vitro transcription using Ambion's miRNA Probe Construction kit (Cat. No.1550, Austin, TX).  We added various amounts of these synthetic miRNAs into the human miRNA samples prior to analysis as internal controls.
manufacture_protocol: as described in CapitalBio lab manual