title: Cattle 7,872-element cDNA
status: Public on Aug 10 2005
contact.city: Urbana
contact.first: Harris
contact.middle: A
contact.last: Lewin
contact.state: IL
contact.address: 1201 West Gregory Drive
contact.department: Animal Sciences
contact.country: USA
contact.web_link: http://cagst.animal.uiuc.edu/Lewin.html
contact.institute: University of Illinois
contact.phone: 217-244-6745
organism: Bos taurus
description: The 7,872 cDNAs used in this microarray were obtained from normalized and subtracted cattle placenta and spleen cDNA libraries (see Band et al., 2002 Anim. Biotechnol. 13:163-172; Everts et al., 2005 Vet. Immunol. Immunopathol. 105:235-245). Positive controls include beta actin (ACTB), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and hypoxanthine phosphoribosyltransferase (HPRT). Exogenous spiking controls are the soybean genes chlorophyll ab binding protein (CAB), Rubisco small chain 1 (RBS1), and major latex protein (MSG). Negative controls are Cot1 DNA, genomic DNA, spotting buffer, poly-A, and water. All PCR products were printed in duplicate on the array.  Keywords: cDNA, cow, liver, bovine, lactation, microarray