title: WT_Rodent_Chip_03
status: Public on Feb 11 2016
contact.city: Loughborough
contact.first: Tim
contact.last: Giles
contact.email: timothy.giles@nottingham.ac.uk
contact.address: College Road
contact.country: United Kingdom
contact.institute: University of Nottingham
organism: Leptospira interrogans
  Campylobacter jejuni
  Francisella tularensis subsp. novicida
  Yersinia enterocolitica
  Yersinia pestis
  Yersinia pseudotuberculosis
  Rickettsia prowazekii
  Rickettsia typhi
  Bartonella quintana
  Staphylococcus aureus
  Mycobacterium avium
  Mycobacterium tuberculosis variant bovis
  Mycobacterium avium subsp. paratuberculosis
  Mycobacterium tuberculosis
  Mycobacterium tuberculosis variant microti
  Toxoplasma gondii
  Cowpox virus
  Streptobacillus moniliformis
  Bartonella henselae
  Mycobacterium canetti
  Escherichia coli O157:H7
  Salmonella enterica subsp. enterica serovar Typhimurium
  Francisella tularensis subsp. tularensis
  Francisella tularensis subsp. holarctica
  Francisella tularensis subsp. mediasiatica
  Paslahepevirus balayani
  Mammarenavirus choriomeningitidis
  Orthohantavirus puumalaense
  Orthohantavirus seoulense
manufacture_protocol: Oligonucleotides were purchased as 3′-amino-modified oligos from Metabion (Martinsried, Germany) and applied at a final concentration of 10 μM in Spotting Buffer 1 (Quantifoil Micro Tools, Jena, Germany). Arrays were spotted using a Microgrid II spotting machine (BioRobotics Inc., Cambridge, UK) according to the instructions of the manufacturer and assembled into ArrayStrips.  Each oligonucleotide probe was replicated two-fold on the array.  The average spot size was 80 μm.