title: Salmonella typhimurium LT2 1.1K
status: Public on Dec 25 2009
contact.city: Fayetteville
contact.first: Arunachalam
contact.last: Muthaiyan
contact.state: AR
contact.address: 2435 N Hatch Ave
contact.department: Food Science
contact.country: USA
contact.institute: University of Arkansas
organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
manufacture_protocol: Using the transcriptome of Salmonella Typhimurium LT2, an oligonucleotide microarray, made up of 43-45 mers, was designed. We choose 1152 genes, that were selected based upon their being associated with virulence or with regulation of virulence genes. Specifications of oligos were based upon various design characteristics such as temperature of melting, 3’ location, specificity, lack of repeat nucleotides, and other consideration as previously described. Oligos were synthesized and normalized in concentration by Integrated DNA Technologies Inc. (Coralville, IA). Oligos were resuspended in Epoxide Slide Spotting Solution and printed onto Epoxide Coated Slides (Corning Inc., Corning, NY) using a GeneMachine’s Omnigrid Accent microarray printer (Genomic Solutions Ann Arbor, MI). Each array also contains duplicate elements for each gene.
  