title: Cotton Ovule cDNA MicroArray ghA
status: Public on Nov 03 2005
contact.city: Canberra
contact.first: Andrew
contact.last: Spriggs
contact.email: andrew.spriggs@csiro.au
contact.state: ACT
contact.address:  GPO Box 1600
contact.department: Bioinformatics Group
contact.country: Australia
contact.institute: CSIRO Plant Industry
contact.phone: 612-6246-5193
organism: Gossypium hirsutum
description: 10584 spot Cotton Ovule cDNA MicroArray
manufacture_protocol: The cotton ovule cDNA microarray comprised a total of 10,410 PCR amplified inserts from cDNA clones. The PCR amplified inserts were purified using ethanol, sodium acetate precipitation. The PCR amplicons were re-suspended in 10 micro liters of 50% DMSO. Quality control of the amplified products was done by separation on agarose gels containing EtBr. Spotting was performed using a VersArray ChipWriter Pro (BioRad) arrayer equipped with 24 Stealth Micro Spotting Pins SMP 3 (Telechem International Inc.). Samples were spotted onto CMT-GAPS II™ coated microarray slides (Corning). Post-printing slide processing was performed by baking the slides at 80 oC for 3 hours.