title: SignatureChip v4.0
status: Public on Feb 27 2011
contact.city: Spokane
contact.first: Blake
contact.middle: C.
contact.last: Ballif
contact.state: WA
contact.address: 2820 North Astor Street
contact.department: Research & Development
contact.country: USA
contact.institute: Signature Genomic Laboratories
organism: Homo sapiens
manufacture_protocol: A total of 1900 BAC clones were selected in contigs of three partially-overlapping clones to target clinically significant regions of interest across the genome. FISH analysis was used to confirm the chromosomal location of each clone and to eliminate clones which showed cross-hybridization to multiple locations. BAC insert DNA was isolated and 5ug of each insert was sonicated to a final size of 50 bp-20 kb and combined with 50% DMSO with nitrocellulose prior to printing. Each insert was printed in quadruplicate onto low-autofluorescence slides using an Omnigrid microarrayer. Printed slides were baked overnight at 80 degrees C, washed with water and 95% ethanol, and stored in a desiccator prior to use.