title: LC Sciences Mouse 01_M9.1_070362 miRNA Array
status: Public on Sep 06 2013
contact.city: New haven
contact.first: seyedtaghi
contact.last: takyar
contact.email: seyedtaghi.takyar@yale.edu
contact.state: CT
contact.address: 300 Cedar street
contact.department: Medicine
contact.country: USA
contact.institute: Yale School of Medicine
organism: Mus musculus
description: Detects miRNA transcripts listed in Sanger miRBase Release 9.1 (http://www.sanger.ac.uk/Software/Rfam/mirna/). Multiple control probes are included in each chip. The control probes are used for quality controls of chip production, sample labeling and assay conditions. Among the control probes, PUC2PM-20B and PUC2MM-20B are the perfect match and single-based match detection probes, respectively, of a 20-mer RNA positive control sequence that is spiked into the RNA samples before labeling. One may assess assay stringency from the intensity ratio of PUC2PM-20B and PUC2MM-20B, which is normally larger than 30. When the option for custom probes is selected, custom probes are also included.
manufacture_protocol: see manufacturer's website