title: NAPPA
status: Public on Jan 10 2024
contact.city: Bronx
contact.first: Jacqueline
contact.middle: M.
contact.last: Achkar
contact.email: Jacqueline.achkar@einsteinmed.edu
contact.state: NY
contact.address: 1300 Morris Park Ave
contact.country: USA
contact.institute: Albert Einstein College of Medicine
contact.phone: 7184302971
organism: synthetic construct
description: 3295 genes from H37Rv and 437 genes CDC1551 were cloned in a pANT7-cGST expression vector, which encodes a C-terminal fusion partner for the target gene of Glutathione-S-Transferase (GST)
manufacture_protocol: The NAPPA print was performed on AU302 piezoelectric dispensing system (Engineering Arts LLC, Tempe, AZ, USA) by depositing MM (1200 pL/well) and plasmid(s) (100 ng/µl, 300 pL/well) sequentially utilizing 16 individual non-contact dispensing heads. The NAPPA slides were stored under an argon gas filled container at room temperature until the day of use when proteins were expressed.