title: Paracoccidioides brasiliensis microarray v1
status: Public on Aug 26 2005
contact.city: Santo André
contact.first: Luiz
contact.middle: R.
contact.last: Nunes
contact.email: luiz.nunes@ufabc.edu.br
contact.state: SP
contact.address: Rua Santa Adélia 166
contact.department: Centro de Ciências Naturais e Humanas
contact.country: Brazil
contact.institute: UFABC - Universidade Federal do ABC
contact.phone: (55) (11) 4798-7104
organism: Paracoccidioides brasiliensis
description: Paracoccidioides brasiliensis isolate 18
manufacture_protocol: The array consists of cDNA inserts from representative ESTs from 4,692 expressed genes that were functionally classified by similarity to known genes. These inserts were PCR amplified with the M13 forward and reverse primers and the amplified products were purified with the QiaQuick 96 PCR purification system (Qiagen), dried in a Savant speed-vac, resuspended in 50 % DMSO at a final concentration of 100-200 mg/ml and spotted onto CMT-GAPS Coated Slides (25 x 75 mm, Corning) using an Affimetrix 427 arrayer. Those interested in obtaining the P. brasiliensis microarrays, please contact lnunes@umc.br or ggoldman@usp.br for further details