title: Carcinus maenas oligo microarray
status: Public on Oct 09 2011
contact.city: Winnipeg
contact.first: Sandra
contact.last: Fehsenfeld
contact.email: umfehsen@cc.umanitoba.ca
contact.state: MB
contact.address: 509 Buller Building, 45 Chancellors Circle
contact.department: Biological Science
contact.country: Canada
contact.institute: University of Manitoba
organism: Carcinus maenas
description: Contig IDs (CMX) according to Towle DW, Henry RP, Terwilliger NB: Microarray-detected cahnges in gene expression in gills of green crabs (Carcinus maenas) upon dilution of environmental salinity. Comp.Biochem. Physiol.D 2010, in press.
manufacture_protocol: 1. Oligonucleotide design: Array Designer 4 software, based on the sequences of the ESTs (50mers)
  2. Oligonucleotide synthesizing: Integrated DNA Technologies, provided in 384-well plates at a concentration of 250 μmol l-1
  3. Microarray printing: to 17 μl of Pronto Universal Spotting Solution (Corning) in a 384-well plate, dispense 3 μl of oligonucleotide solution
  4. adjust OmniGrid Accent [equipped with silica spotting pins (Parallel Synthesis Technologies 116 Inc.]) arrayer: set density and spot pattern desired; chamber humidity to 62-64% silica spotting pins (Parallel Synthesis Technologies  116 Inc.
  5. place 384-well plates containing diluted oligos into position
  6. place slides into the chamber and wait for humindity to stabilize for 30min, then start printing protocol
  7. after printing, dry slides in a vacuum desiccator overnight, then cross-link in a UV Stratalinker (Stratagene) at a setting of 6000 x 100 μJ