title: Pseudomonas fluorescens Pf29Arp microarray
status: Public on Oct 01 2014
contact.city: Champenoux
contact.first: Annegret
contact.last: Kohler
contact.email: annegret.kohler@inrae.fr
contact.address: Centre Grand Est Nancy
contact.country: France
contact.institute: INRAE
organism: Pseudomonas fluorescens
manufacture_protocol: A genomic library containing 14208 clones was PCR-amplified. Amplified DNA products of the 14 208 clones were purified on SephadexTM G-50 superfine (GE Healthcare, Little Chalfont, UK) and multiscreen filter plates (Millipore, Bedford, MA, USA) and then resuspended in 20 μl of spotting buffer (1.5 m betaine, 3X SSC). The PCR efficiency was around 85%, and 93% of the amplified clones had a DNA insert, with 1 kbp average size. We amplified 11 400 PCR fragments, theoretically representing 80% of the genome. DNA products were printed in simplicate on Corning UltraGAPS II slides (Corning, Corning,NY, USA) by a Lucidea spotter (Amersham Biosciences, Piscataway, NJ, USA) at the BioGenouest transcriptomic facilities (Nantes, France)
  Pseudomonas fluorescens Pf29Arp shotgun DNA microarray