title: Heidelberg FlyArray
status: Public on Dec 31 2003
contact.city: Heidelberg
contact.first: Boris
contact.last: Beckmann
contact.email: b.beckmann@dkfz.de
contact.address: Im Neuenheimer Feld 580
contact.department: Funktionelle Genomanalyse
contact.country: Germany
contact.institute: Deutsches Krebsforschungszentrum
contact.phone: +49-6221-424679
organism: Drosophila melanogaster
description: cDNA Microarray from spotted PCR products containing 47,616 features from Drosophila melanogaster and various controls. Primer design was made with GenomePride software. Average length is 500 bp within exonic sequence.
  Microarray construction
  The 2nd round PCR-product was spotted in 3x SSC, 150mM NaPO4, 1.5M betaine onto QMT Amino slides (Quantifoil, Jena, Germany)
  using a MicroGrid II arrayer (BioRobotics, Cambridge, UK) and SMP3 pins (TeleChem International Inc., Sunnyvale, USA).
  Each PCR-product was spotted twice at different positions on the microarray. As controls, PCR-products of Arabidopsis cDNAs,
  genomic Drosophila DNA and C. elegans cDNAs were spotted. The DNA was UV-crosslinked (250mJ/cm2) and baked for 4h at 80°C.
  In total, the Heidelberg FlyArray contains 47,616 features, representing the 21,306 ORF-amplicons and 2,502 controls.