title: Arabidopsis thaliana 34K NARC serie 8
status: Public on Oct 15 2010
contact.city: Trondheim
contact.first: Per
contact.last: Winge
contact.email: per.winge@ntnu.no
contact.address: Høgskoleringen 5e
contact.department: Department of Biology
contact.country: Norway
contact.institute: Norwegian University of Science and Technology
contact.phone: +47 73596229
organism: Arabidopsis thaliana
description: Norwegian Arabidopsis Research Center (NARC)
manufacture_protocol: 1. Oligos used in the production of the microarrays are from the Qiagen-Operon Arabidopsis Genome Array Ready Oligo Set (AROS) Version 3.0, while the custom made oligos were produced by Operon (Alameda, CA, USA) or MWG (Ebersberg, Germany). Information about the AROS probes is available at: http://omad.operon.com/arabidopsis/
  2. Oligos are arrayed in Genetix X6004 384 well plates with V-shaped bottom. Each well contain 600 pmol 70 mer oligo
  3. Oligos were resuspended in 16 µl Milli-Q-filtered DNase free water and 8 µl from each well were transfered to new set of Genetix X6004 384 well plates using a Tecan Genesis RSP 200 liquid handling workstation.
  4. 8 µl DMSO were added to each well giving a final oligo consentration of ~20 µM and 50% DMSO.
  5. The plates were sealed with ABgene Adhesive PCR Foil seals and centrifuged at 1000 rpm in a Sorvall RT6000B centrifuge. The plates were stored at -20C.
  6. The plates were thawed and placed on a rotary shaker for 12 hour before printing of oligos.
  7. The oligos were printed on amino silane coated UltraGaps slides (Corning, NY, USA) using a BioRobotics MicroGrid II robot (Genomic Solutions, MI, USA).
  8. After printing the micorarray slides were exposed for UV light in a Stratalinker 2400 UV crosslinker (Stratagene), at 800 milijoules/cm2.
  9. Microarray slides were stored under low humidity conditions and not exposed to light.