title: Agilent-024081 Hopkins/TRC human shRNA Array
status: Public on Feb 10 2012
contact.city: Taipei
contact.first: Yu-yi 
contact.last: Lin
contact.email: yuyilin@ntu.edu.tw
contact.address: R809, No.1 Jen-Ai Road Section 1
contact.country: Taiwan
contact.institute: National Taiwan University College of Medicine
organism: Homo sapiens
description: Agilent AMADID024081
  TRC website: http://www.broadinstitute.org/rnai/public/ for shRNA sequence search.
manufacture_protocol: Comprehensive array containing TRC1.0 human shRNA half-hairpin probes for deconvolution of mulplex RNAi screen. Each probe contains the specific 21-nucleotide antisense-strand sequence of each shRNA along with one nucleotide immediately flanking both 5’ (a G nucleotide) and 3’ (a C nucleotide) ends. The probes also contained a stretch of 60-nucleotide linker sequence to attach the slide surface, and were randomly distributed across the array. The customized microarray slides were synthesized by Agilent at a density of 4 x 180,000 (4 subarrays containing 180k probes each).