title: Mm-FDA-OperonV4-35K
status: Public on Oct 18 2011
contact.city: Bethesda
contact.first: Amy 
contact.middle: Xiaoyun
contact.last: Yang
contact.email: amy.yang@fda.hhs.gov
contact.state: MD
contact.address: 8800 Rockville Pike
contact.department: Division of Celular and Gene Therapies
contact.country: USA
contact.web_link: http://@fda.hhs.gov
contact.institute: CBER, FDA
contact.phone: 301-827-9770
organism: Mus musculus
manufacture_protocol: The mouse genome oligonucleotide probe set version 4.0 contains 35,852 optimized 70mers (plus controls) obtained from Operon (no more existing). The oligonucleotide probes were purchased in 384-well polypropylene plates (Genetix, 384 micro array) and dissolved in a print buffer (150mM sodium phosphate, pH 8.5, 4% sodium sulfate) at a concentration of 15 uM. The microarray is composed of 48 blocks and one spot is printed per probe per slide. The spot size is 70 micron and spot distance is 155 microns (center-to-center). The microarrays were printed on epoxide coated slides (Corning).