title: MRC Toxicology Unit-Mus musculus-miRCURY LNA f version 8.0 of miRBase (Exiqon)
status: Public on Jun 24 2016
contact.city: Didcot
contact.first: Emma
contact.middle: Louise
contact.last: Marczylo
contact.state: Oxfordshire
contact.address: Harwell Campus, Chilton
contact.department: Toxicology
contact.country: United Kingdom
contact.institute: Public Health England
organism: Mus musculus
description: MRC Toxicology Unit-Mus musculus-miRCURY LNA probe set probe set providing complete coverage of version 8.0 of miRBase (Exiqon)
manufacture_protocol: Probes were supplied dried down in 384 well plates and were resuspended to 20 µM in 3X SSC, 1.5 M Betaine, 30% (v/v) ethylene glycol prior to printing. Plates were loaded onto an ArrayJet microarrayer for printing onto Genetix aldehyde plus slides. Each probe was printed 4 times. The layout of the print was over 3 blocks, each one consisting of 32 columns and 36 rows, with a total of 12 blocks representing n=4 of each probe.