title: Arborea spruce 11K cDNA microarray
status: Public on Dec 01 2010
contact.city: Edmonton
contact.first: Chelsea
contact.last: Ju
contact.email: chelsea.ju@ualberta.net
contact.state: Alberta
contact.address: CW 405, Biological Sciences Centre
contact.department: Biological Sciences
contact.country: Canada
contact.institute: University of Alberta
organism: Picea glauca
manufacture_protocol: The 11K microarray was designed based on the white spruce (Picea gluca [Moench.] Voss) expressed sequence tag (EST) assembly. This assembly comprised 16600 unique contigs sequenced from 17 cDNA libraries, which represented different tissues at a diverse developmental stages and growing conditions.  10400 nonredundant sequences were selected and printed as double, side-by-side on the microarray slide with 4X12 subgrid layout and 132 spots per subgrid.  Details of the microarray manufacture and quality control are described in Pavy et al. (2008) (PMID 18811621).