title: Human miRNA 1K
status: Public on May 10 2012
contact.city: Guangzhou
contact.first: Rongrong
contact.last: wei
contact.email: happyrong1221@gmail.com
contact.address: 651 Dongfeng East Road
contact.department: cancer center
contact.country: China
contact.institute: Sun-Yat Sen university
organism: Homo sapiens
manufacture_protocol: The probes (40 μM final concentration) mixed with printing buffer were printed onto slides in duplicate using SmartArrayer™ 136 printer (CapitalBio Inc, Beijing, China). 2.5 μg of total RNA was employed with 100 nmol/L of pCp-DY647 (Dharmacon, Lafayette, CO, USA) and 15 Units of T4 RNA ligase (USB, Cleveland, Oh, USA) in a total reaction volume of 20 µL at 16 ℃ overnight. The labeled RNAs were mixed and hybridized to the array with a 2×hybridization solution (final concentration: 5× Denhart’s solution, 0.5% SDS, 5× SSC) in a Hybridization Chamber (Corning Inc, Corning, NY, USA) at 46 ℃ for 12 to 16 hours. After washing, the microarray was scanned with a LuxScan™ 10K Microarray Scanner (CapitalBio Inc, Beijing, China) at PMT 800. The scanned image was gridded using the GenPix Pro 6.0 software (Axon Instruments, Foster City, CA, U.S.A.).