title: Yang et. al. Oligoarray of CfMNPV select ORFs
status: Public on May 01 2007
contact.city: Guelph
contact.first: Dan-Hui
contact.last: Yang
contact.email: dyang@uoguelph.ca
contact.state: Ontario
contact.address: 488 Gordon Street
contact.department: Dept. of Molecular and Cellular Biology
contact.country: Canada
contact.web_link: http://www.uoguelph.ca/mcb
contact.institute: University of Guelph
contact.phone: 1-519-8244120 Ext.53264
organism: Choristoneura fumiferana multiple nucleopolyhedrovirus
description: The array included 46 oligonucleotides (1.0 µg/µl) representing 23 CfMNPV ORFs, one for the coding strand and the other for the non-coding strand.  Purified viral genomic DNA (50, 25, 5 ng/µl) was used as a positive control. A mixture of 18 viral oligonucleotides (50 ng/µl each) was served as a second positive control. The array included 2 oligos (At1 and At2) of Arabidopsis chlorophyll synthase gene to be hybridized with the spike RNA, and 2 oligos (HAT4, YaaJ) as negative controls. The array also included 5 insect internal control oligos. Each probe was printed in triplicate.
manufacture_protocol: Spotted 70 mer nucleotides on glass