title: INRA-INSA Rat Operon V3 oligo array
status: Public on Mar 13 2007
contact.city: Toulouse
contact.first: Françoise
contact.last: Guéraud
contact.email: fgueraud@toulouse.inra.fr, marques@toulouse.inra.fr
contact.address: 180, Chemin de Tounefeuille BP3, Cedex 9
contact.department: UMR1089 INRA-ENVT
contact.country: France
contact.institute: Institut National de la Recherche Agronomique (INRA)
contact.phone: +33 5 61 28 5383
organism: Rattus norvegicus
description: The Rat_V3 array-ready oligo set (AROS) is based in the ENSEMBL rat v19.3b.2 database. This geneliste provides annotation based on the ENSEMBL rat v19.3b.1 database (http://ensemble.org).
manufacture_protocol: Rat oligos (set_V3 Operon, 73 plates/well) were loaded at 300 pmol/well. Oligos were resuspenden in 20 µl 3X SSC buffer (final concentration 20 µM). Plates were shaken and frozen. Prior to spotting, plates are defrozen one hour at room temperature and centrifuged. Spotting was performed as single spot on Ultra GAPS glass slides (CORNING) using Qarray mini robot (GENETIX). Atmosphere moisture was set at 50% and plates were cooled to 15ºC to prevent drying. Spotting design was as follow: 48 pins therfore 48 grids, each grid contains 24X25 spots