title: Human 9K CGI Array
status: Public on Jan 22 2010
contact.city: Beijing
contact.first: Kunlin
contact.last: Zhang
contact.address: DaTun Road A4
contact.country: China
contact.institute: Chinese Academy of Sciences
organism: Homo sapiens
manufacture_protocol: Clone:pGEM-5Zf(-)/Primers:5'-CGG CCG CCT GCA GGT CGA CCT TAA- 3';5'-AAC GCG TTG GGA GCT CTC CCT TAA- 3';oligo synthesize/strandedness:Methylation tolerant E.coli strain XLI-BLUE MRF'/Length:760 bp(Average insert size);Arrayer Harderware:Aminosilane Coated Glass Slides/Software:Arrays were scanned with a confocal LuxScanTM scanner (CapitalBio Corp.), and obtained images were analyzed with LuxScan3.0 TM software (CapitalBio Corp);Spotting protocol:The PCR products were precipitated with ethanol and re-dissolved in EasyArrayTM Spotting Solution (CapitalBio Corp.), then printed in triplicate on Aminosilane Coated Glass Slides using a SmartArrayTM microarrayer (All from CapitalBio Corp.).