title: NADC MAP Oligo v1.0
status: Public on Feb 08 2006
contact.city: Ames
contact.first: Mike
contact.last: Paustian
contact.email: mpaustia@nadc.ars.usda.gov
contact.state: IA
contact.address: 2300 Dayton Ave
contact.country: USA
contact.institute: National Animal Disease Center, USDA-ARS
organism: Mycobacterium avium subsp. paratuberculosis
  Mycobacterium avium subsp. avium
manufacture_protocol: 70mer oligos were designed using ArrayOligoSelector. Input sequences included all annotated ORFs and intergenic regions > 500 bp from MAP K10 as well as all MAA 104 ORFs with < 30% identity to MAP K10. MAA 104 sequence was obtained from TIGR on 07/09/04 and automatically annotated using Reganor software (McHardy et al). One oligo was designed for each ORF < 2000 bp and two oligos were designed for each ORF > 2000 bp. Six Arabidopsis clones and MAP K10 sheared genomic DNA are included as controls. Oligos were synthesized by Illumina. The microarray was printed using a BioRobotics arrayer equiped with 48 quill tip pins. Each source well was printed in triplicate on the array.