title: Li UCSF yeast 14062 v1.0
status: Public on Mar 17 2006
contact.city: San Francisco
contact.first: Joachim
contact.middle: J
contact.last: Li
contact.email: joachim.li@ucsf.edu
contact.state: CA
contact.address: 600 16th Street S376
contact.department: Microbiology and Immunology
contact.country: USA
contact.institute: University of California, San Francisco
contact.phone: 415-476-8782
organism: Saccharomyces cerevisiae
manufacture_protocol: PCR products representing ORF and intergenic yeast genomic sequences were amplified.  Products were spotted on poly-L-lysine coated glass slides using a linear servo arrayer from the DeRisi Lab at UCSF.
  
  Blast analysis of oligos sequences was used to determine the position of the final PCR product. Those oligo pairs which Blast analysis indicated could not generate a product or generated more than one product given the flag –1.  In cases where no product was found the fields Chr, Start and End have been left empty.  In cases where multiple products were found values in the fields Chr, Start and End correspond to the first product identified.
  
  For all elements (except mitochondrial) the sequence of the PCR product was determined using the positions determined above.  Blast analysis of the PCR products was used to determine how repetitive each product was.  Elements that had products that were too repetitive were given a flag of –2.  The sequence of these products is listed.
  
  Elements with flags of –1 or –2 were removed from all datasets prior to analysis.
  
  Lamda DNA spots were not included in analysis.