title: Dichelobacter nodosus oligonucleotide microarray
status: Public on Apr 17 2006
contact.city: Melbourne
contact.first: Julian
contact.middle: I
contact.last: Rood
contact.email: julian.rood@monash.edu
contact.state: Victoria
contact.address: Wellington Road
contact.department: Microbiology
contact.country: Australia
contact.institute: Monash University
contact.phone: 61 3 990 29157
organism: Dichelobacter nodosus
description: Microarrays consisted of 2,317 custom 70-mer oligonucleotides (designed to reduce cross-hybridisation) spotted in triplicate on Ultra-GAPS slides (Corning) using a QArray Mini (Genetix) arrayer, representing 1,343 ORFs as determined from the unpublished D. nodosus genome sequence (http://www.tigr.org) and where possible two gene probes per ORF were synthesised.  Oligonucleotides were spotted on a 2X6 metagrid, which was composed of 24X26 subgrids.
manufacture_protocol: Microarrays were constructed in house and consisted of 2,317 custom 70-mer oligonucleotides (designed to reduce cross-hybridisation; Gary Myers and Ian Paulsen, TIGR) spotted in triplicate with 50% DMSO on Ultra-GAPS slides (Corning) using a QArray Mini (Genetix) arrayer, representing 1,343 ORFs, as determined from the unpublished D. nodosus genome sequence (http://www.tigr.org).