title: Human 10k cDNA Array
status: Public on Oct 27 2014
contact.city: Fort Detrick
contact.first: seid
contact.last: muhie
contact.email: yimersm@gmail.com
contact.state: MD
contact.address: 568 Doughten Dr
contact.department: Systems Biology Enterprise
contact.country: USA
contact.institute: USACEHR
organism: Homo sapiens
manufacture_protocol: The PCR products, ranging between 500 to 700 base pairs, were deposited in 3x saline sodium citrate (SSC) at an average concentration of 165 ug/ml on CMT-GAPSTM II (γ-aminopropylsilane) coated slides (Corning Inc., Corning, NY), using VersArray™ MicroArrayer (Bio-Rad, Hercules, CA).  The cDNAs were UV-cross-linked at 120 mJ/cm2 using UV Stratalinker 2400 (Stratagene, La Jolla, CA).  The microarrays were baked at 80C for four hours.  The slides were treated with succinic anhydride and N-methyl-2-pyrrolidinone to remove excess amines.