title: NimbleGen Polaromonas JS666 385K array v1.0 (condensed)
status: Public on Dec 01 2009
contact.city: Ithaca
contact.first: James
contact.middle: M
contact.last: Gossett
contact.email: jmg18@cornell.edu
contact.state: NY
contact.address: 220 Hollister Hall
contact.department: Civil and Environmental Engineering
contact.country: USA
contact.institute: Cornell University
organism: Polaromonas sp. JS666
description: Whole-genome expression arrays were synthesized by NimbleGen Systems, Inc.  Arrays were designed to obtain full coverage of open-reading frames and a 50-bp tiling of the intergenic regions.  A frequency filtering of the intergenic regions was carried out to remove low-complexity regions and repeats.  Long oligos (60-mer) were synthesized on ultra high density, 1-plex arrays containing 385,000 features.  The arrays contained five replicate probes for each of the target sequences. A condensed template is presented below in the Platform data table.
  Native NimbleGen files (ndf, ngd) are linked below as supplementary files.
manufacture_protocol: High-density DNA array prepared with Maskless Array Synthesizer (MAS) technology. See manufacturer's website at http://www.nimblegen.com/.