title: CombiMatrix_Influenza A_12,000_design 5200
status: Public on Nov 01 2008
contact.city: Mukilteo
contact.first: Michael
contact.middle: Joseph
contact.last: Lodes
contact.email: mlodes@combimatrix.com
contact.state: WA
contact.address: 6500 Harbour Heights Pkwy, Suite 303
contact.department: Assay Development
contact.country: USA
contact.institute: CombiMatrix Corp
contact.phone: 425-493-2272
organism: Influenza A virus
description: Semiconductor based array with in situ synthesized oligonucleotides that can be used with electrochemical detection.
manufacture_protocol: The custom oligonucleotide microarray is synthesized on a semiconductor using an electrochemical synthesis process. Each oligonucleotide probe (spot) is synthesized on a platinum electrode that is independently controlled by the synthesizer’s computer. Synthesis is based on established phosphoramidite chemistry and occurs at thousands of sites simultaneously according to a computer algorithm that activates only specified electrodes. Since physical photolithographic masks, mechanical micromirrors, or inkjet technologies are not involved in the process, all probes can be easily changed without extra time or cost.
  The oligonucleotides are synthesized in a proprietary porous reaction layer that coats the surface of the semiconductor, and acts to covalently attach the oligonucleotide to the surface of the chip.
  Influenza A subtypes H1-15 and N1 - 9