title: NimbleGen human CGH 4x72K Whole Genome Array [80101_HG18_WG_CGH_v2_X4]
status: Public on Jul 29 2011
contact.city: Madison
contact.first: Inc
contact.last: Nimblegen Systems
contact.email: geo@ncbi.nlm.nih.gov
contact.state: WI
contact.address: 504 S. Rosa Road
contact.country: USA
contact.web_link: http://www.nimblegen.com/
contact.institute: NimbleGen Systems, Inc.
contact.phone: 608-218-7600
organism: Homo sapiens
description: design ID : 6916
  native array description file : 080101_HG18_WG_CGH_v2_X4.ndf
  native array description file : 080101_HG18_WG_CGH_v2_X4.pos
manufacture_protocol: NimbleGen human CGH 4x72K Whole Genome v2.0 array (Roche NimbleGen, Inc). Placenta DNA was used as the reference. The CGH array protocol from NimbleGen Systems was followed. Briefly, 500 ng of tumour and reference DNA were labelled with Cy3 and Cy5, respectively. Labelled material was co-hybridized to microarrays consisting of 71341 oligonucleotide probes spaced at approximately 40000 bp intervals throughout the human genome, washed and scanned at 10 µm resolution using the GenePix 4000B dual scanner (Axon Instruments, Burlingame, CA). Raw data were extracted using NimbleScan software v2.5 (Roche NimbleGen, Inc), which enables automated grid alignment, extraction and generation of data files.