title: STREM platform (Stress Response Microarray in Mytilussp)
status: Public on Aug 17 2017
contact.city: Padova
contact.first: Gerolamo
contact.last: Lanfranchi
contact.email: stefano.cagnin@unipd.it
contact.address: Via U. Bassi, 58/B
contact.department: CRIBI - Biotechnology Center and Biology Department
contact.country: Italy
contact.institute: University of Padova
contact.phone: +39-0498276219
organism: Mytilus californianus
  Mytilus edulis
  Mytilus galloprovincialis
  Magallana gigas
manufacture_protocol: STREM platform was constructed arraying on glass slides 465 DNA oligo  corresponding to the 3’-portion of a set of 16 biological processesat cellular:  Lysosomal activity, DNA Metabolism, Translation, Mitochondrial activity, Carbohydrate metabolism, Heat choc response, Oxydatif stress, Proteolysis, Phase I and II detoxification, Metal resistance, Endocrine disruptors, Endocytosis,  Macrophage, Pumps, Cytoskeleton and  Adhesion to substrate. The probes are triplicated randomly to fill a 1395-feature sub-microarray for a total of 8 sub-arrays per slide.  All sequences are from Mytilus californianus,  galloprovincialis, edulis and CrassosteraGigas . Spotting was performed using the robotic system Genpak Array 21 (Genetix, Hampshire, UK) equipped with 6 Stealth Micro Spotting Pins SMP 3B (ArrayIt) settled to obtain spots with an average diameter of 90 µm.  Spotting was performed at 50% relative humidity to obtain the best spot morphology and to reduce the plate evaporation. Microarrays were then processed in a UV cross linker (Stratagene, La Jolla, CA, USA) (total power of 300 mJoules) for the binding the DNA to the slides. Microarrays were finally processed to remove any unbound product, dried and stored under vacuum at room temperature in sealed boxes.