title: Thermococcus gammatolerans microarray
status: Public on Jul 31 2012
contact.city: Orsay
contact.first: Arnaud
contact.last: Lagorce
contact.email: arnaud.lagorce@igmors.u-psud.fr
contact.address: Université Paris-Sud 11
contact.department: Institut de Génétique et de Microbiologie
contact.country: France
contact.web_link: http://www.igmors.u-psud.fr/
contact.institute: University Paris Sud
contact.phone: +(33) 01 69 15 46 21
organism: Thermococcus gammatolerans
manufacture_protocol: Array design and printing
  Microarray DNA probes were designed using Primer3 Software available at the website http://primer3.sourceforge.net/ (Rozen, 2000). This program designs optimal microarray oligonucleotides of all genomic ORFs. The parameters set in the program included a probe length range between 49 and 51-mers (optimal 50mers), primer melting temperature range of 70 to 90°C (optimal: 80°C), and a BLAST identity score of 100. Oligonucleotides defined by Primer3 were synthesized by Sigma-Proligo and printed with the MicroGrid II arraying robot (Biorobotics) on commercial HydroGel slides (Schott, Germany). Quality of each microarray batch was checked using sybr green.