title: TIGR_RAT_27k_cDNAarray_1
status: Public on Mar 04 2008
contact.city: Washington, DC
contact.first: Norman
contact.middle: H
contact.last: Lee
contact.email: phmnhl@gwumc.edu
contact.state: DC
contact.address: 2300  I Street, N.W.
contact.department: Pharmacology & Physiology
contact.country: USA
contact.institute: The George Washington University Medical Center
organism: Rattus norvegicus
description: The arrays are printed on the barcoded side of the slide with approximate distance from the top, left side and right side of 3 mm. The array consists of 48 grids with each grid representing 576 spots in a 24 x 24 arrangement.
manufacture_protocol: TIGR Department of Mammalian Genomics protocol.
  The TIGR RAT 27k cDNAarray consists of 26,501 probe elements representing 20,465 unique genes.
  Purified cDNA amplicons were combined with the printing buffer to a final  buffer percentage of 50% DMSO. The printing plates were arrayed onto aminosilane glass slides on a custom-built microarray robot from Brooks Automation (Cambridge, MA). Printed arrays were UV cross-linked to ensure binding.