title: Ocimum Biosolutions Human 40k OciChip A
status: Public on Jul 31 2011
contact.city: Kolkata
contact.first: Nitai
contact.middle: Pada
contact.last: Bhattacharyya
contact.email: nitai_sinp@yahoo.com
contact.state: West Bengal
contact.address: 1/AF Bidhannagar
contact.department: Crystallography and Molecular Biology division
contact.country: India
contact.institute: Saha Institute of Nuclear Physics
organism: Homo sapiens
manufacture_protocol: Array Design Protocol: Human 40K OciChip consisting of 20,160 spots was printed on Corning® epoxy-coated glass slides using Omnigrid (Gene Machines). Chip has been printed using 4x12 SMP4 pins on 75.5x 25.5x1.3mm microarray glass slides. The origin offset values were 0 for X direction and 0 for Y direction. The dot to dot distance is 220 um; distance between subarrays is 150um and no of dots per array is 20x21=420. Oligos were printed using spotting buffer A from Ocimum Biosolution at 50% humidity and temperature at 22°C. The time taken by the robotic gridder to print 20160 spots was 25 hrs. Each probe was checked for its performance by standard QC process followed at Ocimum Biosolutions. Printed slides were stored in a light-tight box in a bench-top dessicator, with desiccant and were kept at 4 deg C until they were used for hybridization.