title: Sus scrofa Swine Leucocyte Antigen region Tiling Array 385K Nimblegen
status: Public on Oct 15 2009
contact.city: Jouy-en-Josas
contact.first: Sylvain
contact.last: Marthey
contact.email: abridge@inra.fr
contact.address: Domaine de Vilvert Bat 320
contact.department: Animal Genetics
contact.country: France
contact.institute: INRA
organism: Sus scrofa
description: The 385k SLA Tiling Array was designed in both orientations to cover the SLA complex. The sequence from olfactory receptor gene OLF42 to BTNL6 on SSC7 p arm that belong to Class I and III regions was gotten from VEGA database on 16 May 2007 release (http://vega.sanger.ac.uk/Sus_scrofa/index.html) including 1,795,000 nt, and the sequence from centromeric sequences to RING1 on SSC7 q arm that belong to Class II region was gotten from VEGA database on 16 May 2007 release and SBAB BACs including 974,684 nt. The tiling array also covered the sequence belonging to centromeric/pericentromeric region and it was gotten from SBAB BACs, which were derived from a Large White boar homozygous for the H01 haplotype including 241,770 nt.
manufacture_protocol: Maskless array synthesis was used to construct isothermal arrays. An isothermal format (Tm=76oC) and probe length constraint between 50 and 75 bp were used for probe synthesis. Each probe overlapped its neighbor by about 40 base pairs.