title: Huang Lab Human 9K CpG Island Spotted Array
status: Public on Dec 01 2009
contact.city: Columbus
contact.first: Samson
contact.middle: T.
contact.last: Jacob
contact.email: samson.jacob@osumc.edu
contact.state: OH
contact.address: 420 W. 12th Ave
contact.country: USA
contact.institute: Ohio State University
organism: Homo sapiens
manufacture_protocol: We used the Affymetrix/GMS 417 microarrayer to dot unpurified PCR products (~0.02 μl per dot 0.1μg/μl) in the presence of 20% DMSO as microdots (150-μm diameter spaced at 300 μm) on poly-L-lysine-coated microscope slides. Spotted DNA was denatured before use.