title: NHGRI Human 30k cDNA array
status: Public on Mar 31 2011
contact.city: Bethesda
contact.first: Phillip
contact.middle: A
contact.last: Dennis
contact.state: MD
contact.address: 37 Convent Dr.
contact.department: MOB, CCR
contact.country: USA
contact.web_link: http://ccr.cancer.gov/staff/staff.asp?Name=dennis
contact.institute: NCI
contact.phone: (301)-496-0929
organism: Homo sapiens
manufacture_protocol: The cDNA microarrays contained over 28,798 cDNA clones representing over 23,600 unique UniGene clusters. These cDNA clones were obtained from the sequence-verified IMAGE clone selection from Research Genetics (Invitrogen Corporation, Inc). After PCR amplification and purification, the cDNA inserts were resuspended in 3x SSC solution and spotted on poly-lysine-coated slides. The slides were then blocked by regular succinic Anhydre solution, denatured in boiling water and dried by centrifugation. The gene names are listed according to UniGene build 141 (www.ncbi.nlm.nih.gov/UniGene).