title: IQ-USP Xylella fastidiosa 9.2K v2.0.
status: Public on Jan 06 2010
contact.city: São Paulo
contact.first: Paulo
contact.middle: A.
contact.last: Zaini
contact.email: paulo.zaini@gmail.com
contact.state: São Paulo
contact.address: Av. Prof. Lineu Prestes, 748 - Butantã
contact.department: Bioquímica
contact.country: Brazil
contact.institute: Universidade de São Paulo
organism: Xylella fastidiosa
manufacture_protocol: A 9,216-element DNA microarray was printed containing unique internal fragments of 2,608 CDS spotted at least in duplicate, representing 91.6% of all of the 2,848 CDS annotated by Simpson et al. (2000). DNA fragments ranging from 200 to 1,000 bp were PCR amplified with CDS-specific primers (18- to 23-mers) designed with PRIMER3 (http://www-genome.wi.mit.edu/genome_software/other/primer3.html) and based on the annotated genome sequence of X. fastidiosa strain 9a5c. PCR products in 50% DMSO were spotted with a Generation III Spotter (GE Healthcare, Little Chalfont, England) and kept in low humidity (<15%) until use.