title: Microarrays of Bacillus encoded tRNAs [2020_bacillus.gal]
status: Public on Aug 18 2021
contact.city: Hamburg
contact.first: Zoya
contact.last: Ignatova
contact.email: zoya.ignatova@chemie.uni-hamburg.de
contact.address: Martin -Luther-King-Platz 6
contact.country: Germany
contact.institute: Universitaet Hamburg
organism: Enterococcus faecalis
manufacture_protocol: 1. Oligos were arrayed in 384 well plates with a final concentration of 10 µM in 3× SSC/0.01% SDS in a total volume of 25 µl.
  2. Arrays were printed onto (3-Glycidyloxypropyl)trimethoxysilane (Sigma) activated glas slides using a contact-spotter (QArray 2, Moleculare Devices).
  3. After spotting arrays were incubated overnight at 4°C in a humidified atmosphere and subsequently dried for 30 min at 30°C.
  4. Arrays were stored in a dry atmosphere at room temperature until use.