title: Colorado State University Mycobacterium tuberculosis V20071007
status: Public on Sep 22 2009
contact.city: Baltimore
contact.first: Petros
contact.last: Karakousis
contact.email: petros@jhmi.edu
contact.state: MD
contact.address: 1550 Orleans street
contact.department: Medicine
contact.country: USA
contact.institute: John Hopkins University school of medicine
contact.phone: 4105028233
organism: Mycobacterium tuberculosis H37Rv
description: The Tuberculosis Genome Oligo Set consists of 70mer probes representing 4,269 M. tuberculosis (H37Rv and CDC1551) genes, plus 26 controls. The probes were designed from the predicted genomic sequence of the best-characterized M. tuberculosis strain, H37Rv, obtained from the Sanger Center (Cambridge, UK) and The Institute for Genomic Research (Rockville, MD, USA). An additional 371 probes were designed from the CDC1551 strain. The probes are Tm normalized to 79°C.
manufacture_protocol: Oligonucleotide 70mers were generated from whole genome sequence within coding regions. Features were designed to have similar hybridization temperatures. Samples were spotted and pre-processed using standard procedures from the lab of Rocky Mountain Regional Center of Excellence.