title: UMGC Custom Multispecies miRNA microarray
status: Public on May 08 2021
contact.city: Nanjing
contact.first: Yan
contact.last: Zeng
contact.email: zengyan@njau.edu.cn
contact.state: Jiangsu
contact.address: XuanWu District, No.1 WeiGang
contact.country: China
contact.institute: Nanjing Agricultural University
organism: synthetic construct
description: C. elegans, Drosophila, zebrafish, mouse, rat, and human
manufacture_protocol: A miRNA probe set was purchased from Invitrogen, which was designed based on the Sanger miRBase Sequence Database, Release 9.0.  The set contains approximately 1,140 oligonucleotides of 34 to 44 nt long as probes.  They are complementary to C. elegans, Drosophila, zebrafish, mouse, rat, and human miRNAs, and also include a number of internal and negative control probes.  The oligonucleotides were dissolved in 3 x SSC and quadruply printed on Corning® GAPSTM II coated slides  using a BioRobotics Microgrid II spotter