title: UNAM Human 35K
status: Public on Aug 05 2020
contact.city: San Nicolás de los Garza
contact.first: María
contact.middle: Del Rayo
contact.last: Camacho-Corona
contact.email: maracamcor@gmail.com
contact.state: Nuevo León
contact.address: Av. Universidad S/N, Ciudad Universitaria
contact.department: Facultad de Ciencias Químicas
contact.country: Mexico
contact.institute: Universidad Autónoma de Nuevo León
contact.phone: +5218110395600
organism: Homo sapiens
manufacture_protocol: 1. Human 70-mer oligo library from OPERON Oligo Sets (http://omad.operon.com/) were resuspended to 40M in Micro Spotting solution (Telechem International Inc.).
  2. SuperAmine coated slides 25x75 mm (TeleChem International INC) were printed in single copy, and fixed at 80C for 4 hours.
  3. For pre-hybridization the slides were re-hydrated with water vapor at 60C, and fixed with two cycles of UV light (1200J).
  4. After boiling for two minutes at 92C, slides were washed with 95% ethanol for one minute and prehybridzed in 5X SSC, 0.1% SDS and 1% BSA for one hour at 42C .
  5. The slides were washed and dried for further hybridization.
  6. Printing geometry: Grid “X” and “Y” coordinates in slide 3.5 and 7 mm, dot spacing “Y” 160 micron, dot spacing “X” 155 micron, number of dots per grid “X” 28 and “Y” 27, distances between grids “X” and “Y” 4.5 mm, number of grids 48.