title: PEPperCHIP Autoimmunity Microarray 2.0
status: Public on Feb 28 2015
contact.city: Birmingham
contact.first: Jennie
contact.last: Hamilton
contact.state: AL
contact.address: 1825 University Blvd.
contact.department: Department of Medicine, Division of Clinical Immunology and Rheumatology
contact.country: USA
contact.institute: University of Alabama Birmingham
organism: Helicobacter pylori
  Escherichia coli
  Klebsiella pneumoniae
  Proteus mirabilis
  Shigella flexneri
  Yersinia enterocolitica
  Haemophilus influenzae
  Chlamydia trachomatis
  Porphyromonas gingivalis
  Staphylococcus aureus
  Streptococcus pyogenes
  Lactobacillus delbrueckii subsp. bulgaricus
  Mycobacterium avium
  Mycobacterium tuberculosis variant bovis
  Mycobacterium avium subsp. paratuberculosis
  Mycobacterium tuberculosis
  Mycobacterium gordonae
  Trypanosoma cruzi
  Homo sapiens
  Mus musculus
  Human alphaherpesvirus 1
  Human alphaherpesvirus 2
  Human betaherpesvirus 5
  Human betaherpesvirus 6
  human gammaherpesvirus 4
  Human herpesvirus 4 strain B95-8
  Hepatitis C virus isolate HC-J6
  Human immunodeficiency virus 1
  Human T-cell leukemia virus type I
  Human T-cell lymphotrophic virus type 1 (isolate MT-2)
  Coxsackievirus A9
  Coxsackievirus B4
  Human herpesvirus 4 type 2
  Human adenovirus 12
  Human herpesvirus 4 type 1
  Coxsackievirus A20
  Human endogenous retrovirus K
  Human Endogenous Retrovirus IDDMK1,2-22
  Torque teno virus
  Chlamydia pneumoniae
  Prochlorococcus marinus str. MIT 9202
  Human rotavirus MP409
  Coxsackievirus B4 (strain E2)
  Paraburkholderia fungorum
  Porphyromonas gingivalis W83
  Mycobacterium avium subsp. paratuberculosis K-10
  Human poliovirus 3 strain Sabin
  Staphylococcus aureus A9635
  Betapolyomavirus macacae
  Hepacivirus hominis
description: Laser printer spotted peptides
manufacture_protocol: Amino acid microparticles are laser printed onto a glass slide with a custom 24-color laser printer.  One layer of an amino acid particle pattern is printed on a coated glass slide. A heating step melts the particles.  Embedded amino acids can couple to the chip surface.  In accordance with solid phase Fmoc chemistry, a cycle of synthesis is completed after removal of excessive material by washing and cleavage of the N-terminal Fmoc protection group. The cycle is repeated until the full peptide microarray is synthesized.