title: NHGRI Custom cDNA Microarray
status: Public on Jun 24 2004
contact.city: Gaithersburg
contact.first: Jeff
contact.last: Kiefer
contact.email: jkiefer@tgen.org
contact.state: MD
contact.address: 20 Firstfield Rd,  Suite 110
contact.department: CDDL
contact.country: USA
contact.institute: Translational Genomics Research Institute (TGen)
contact.phone: 240-631-1607 ext 240
organism: Homo sapiens
description: This cDNA microarray encompasses ~16,000 genes printed in the Microarray Core of the the National Human Genome Research Institute (NHGRI)/NIH. These cDNA microarrays were generated from cDNA clones obtained from the sequence verified IMAGE clone selection from Research Genetics (Invitrogen corporation, Inc). Of these clones, 75% represent genes with functional annotation and the remaining represent ESTs and hypothetical proteins. The gene description of these clones was based on the Unigene database, build #148 and the chromosomal base location of these genes was updated from University of California Santa Cruz’s (UCSC) Genome Browser database. This update was performed through the GenBank accession ID of the Image clone ID. For quality control, 2 slides per lot were hybridized for spot consistency, homogeneity and signal to noise ratios.
  Keywords = cDNA Microarray