title: NCSU_chicken_JS1 (96 oligos)
status: Public on Jan 31 2008
contact.city: Raleigh
contact.first: Jean
contact.middle: Eduardo
contact.last: de Oliveira
contact.state: NC
contact.address: 2711 Founders Dr
contact.department: Poultry Science
contact.country: USA
contact.web_link: http://www.ncsu.edu/ashwell-lab/
contact.institute: North Carolina State University
contact.phone: 919-515-5537
organism: Gallus gallus
manufacture_protocol: 1.70-mer oligos synthetized by Operon Biothechnologies Inc. Oligos were resuspended in Tris buffer; 250 µM of each oligo was added to a well of a 384-well plate and dried.
  2. Oligos were resuspended in Universal Spotting Solution (Corning, Inc)
  3. Seal plates with Corning seals.
  4. Shake plate at mid speed on plate vortexer for 1 minute.
  5. Remove seal and place plate on appropriate place on VersArray ChipWriter array printer.
  6. Place UltraGaps Amino-Silane Coated Slides on appropriate place on array printer
  7. Run pin clean-up steps 5 times before start printing (using 1 pin)
  8. Start printing run
  9. Turn-off printer once run is completed
  10. Take plate from printer, seal it with Corning seal and store it at -20C
  11. Let slides dry on printer for 24h
  12. Crosslink slides on UV over set to 6000 x 100 µJ/cm2, store slides on secator