title: Buck Institute_Homo sapiens_25K_verC
status: Public on May 23 2007
contact.city: Hamilton
contact.first: Adeel
contact.last: Safdar
contact.email: safdars@mcmaster.ca
contact.state: ON
contact.address: 1200 Main Street West
contact.department: Kinesiology
contact.country: Canada
contact.institute: McMaster University
contact.phone: 905-525-9140
organism: Homo sapiens
manufacture_protocol: Print plate cDNA was amplified with a standard PCR protocol. cDNA samples were purified and verified with a gel. Purification and gel used robotics (Tecan and Quadra) to process 384-well plates. After purification, cDNA samples were dried down in a speed vacuum and resuspended in a 3XSSC+1.5M Betaine print buffer. Using a Gene Machines Omni grid printer and Telchem Stealth print pins, samples were arrayed in a 32 block, print format on MGM epoy slides.