title: UMDNJ_Human_BCA1135
status: Public on Feb 01 2007
contact.city: Shanghai
contact.first: Guohong
contact.last: Hu
contact.email: ghhu@sibs.ac.cn
contact.address: 320 Yueyang Road
contact.country: China
contact.institute: Shanghai Institute of Nutrition and Health
organism: Homo sapiens
manufacture_protocol: The oligonucleotides were printed in duplicate spots with a center-to-center distance of 250 µm. Microarray preparation was performed according to a procedure established in our laboratory. Briefly, (1) preparation of microarray slides: Pre-cleaned Gold Seal Micro slides (Becton Dickson) without scratches were chosen, and were soaked in 30% bleach with shaking for 1-2 hrs followed by rinsing 5 times with deionized H2O and 3 times with MilliQ H2O. Slides were then sonicated in 15% Fisher brand Versa-Clean Liquid Concentrate with heat on for 1-2 hrs followed by rinsing with shaking in deionized H2O 10 times or until no bubbles occurring and 5 times with MilliQ H2O. Slides were dried by centrifugation at 1,000 rpm for 5 min in a GS-6 Beckman centrifuge with a slide holder and then were baked at 140oC vacuum oven for 4-5 hrs (Fisher Scientific Model 280A); (2) microarray preparation: oligonucleotides were mixed with microarray printing solution (GenScript, PiscatawayNJ) at a 1:5 ratio (v/v) to have a final concentration of 50 µM for each probe in the wells of 384-well plates. Probes were then spotted onto the washed glass slides under a humidity of 50% to 55% and temperature from 24.5oC to 26.5oC