title: GAP_MAF1
status: Public on Dec 09 2004
contact.city: Raleigh
contact.first: Ahmad
contact.last: Fakhoury
contact.email: amfakhou@siu.edu
contact.state: NC
contact.department: Plant Pathology
contact.country: USA
contact.institute: North Carolina State University
contact.phone: 919-515-6995
organism: Aspergillus flavus
description: PCR products were generated using approximately 5 ng plasmid DNA isolated from unique cDNA clones. T3 and T7 primers were used to generate amplicons. PCR products were then purified by isopropanol precipation followed by a 70% ethanol wash. The presence of amplicons was verified by agarose gel electrophoresis.
  PCR products at approximately 250 ng/ul in 50% DMSO were spotted in triplicate onto onto Corning CMT-GAPS glass slides (Corning, NY), using an Affymetrix 417 Arrayer (Santa Clara, CA). Spots were allowed to air dry before being fixed following manufacturer’s recommendation.