title: Bordetella pertussis Whole genome array
status: Public on Oct 07 2014
contact.city: Lille
contact.first: David
contact.last: Hot
contact.email: david.hot@pasteur-lille.fr
contact.address: 1 rue du professeur Calmette
contact.department: Center for Infection and Immunity of Lille
contact.country: France
contact.institute: Institut Pasteur de Lille
organism: Bordetella pertussis
manufacture_protocol: Long oligonucleotide probes were designed on the sequences of the 3554 open reading frames (all coding CDS except transposases of IS elements) of B. pertussis Tohama I genome using OligoArray v2.1 [1]. Oligonucleotides were synthesized by Sigma-Aldrich and spotted on duplicat on Nexterion AL slides (Schott Nexterion) in 1x SciSpot-AM buffer (Scienion) using a Q-Array II spotter (Genetix).