title: COFC human tRNA array 384 v1.0
status: Public on Jul 07 2021
contact.city: Charleston
contact.first: Renaud
contact.last: Geslain
contact.state: SC
contact.address: 58 Coming Street
contact.department: Biology
contact.country: USA
contact.institute: College of Charleston
organism: Homo sapiens
manufacture_protocol: tRNA microarrays were printed manually on amine coated-slides glass slides (Molecular Devices, K2615) with 48 synthetic DNA oligonucleotides (IDT) at 50 µM in 3 X SSC, 0.01% (w/v) SDS using a commercial eight-pin arrayer and a glass slide indexing system (V&P Scientific, Inc., VP 470 and VP 478 respectively). Printed array were crosslinked at 254 nm with 999,999 µJ/cm2 on a Spectroline UV crosslinker (Select series 254 nm) and blocked overnight in 450 mL of blocking solution (125 mM phosphate buffer (NaH2PO4/Na2HPO4-) pH 7.2, 2.25 % SDS (w/v), 0.5 mM EDTA, 0.5 X SSC and 1 % BSA (w/v)) on a magnetic stirrer, at room temperature and under slow agitation. Prior to hybridization blocked arrays were rinsed in boiling water and dried by centrifugation. For a complete, step-by-step protocol, please refer to Emetu et al., 2018 (doi: 10.3791/56898)