title: KTH Human 30k v1.0
status: Public on Dec 11 2008
contact.city: Stockholm
contact.first: Johan
contact.last: Lindberg
contact.email: johlin@kth.se
contact.address: Roslagstullsbacken 21, Floor 3
contact.country: Sweden
contact.institute: Royal Institute of Technology
organism: Homo sapiens
manufacture_protocol: Version 3.0 of the Human Genome Oligo Set contains 34,580 70mer probes representing 24,650 genes and 37,123 gene transcripts. The design is fully based on the Ensambl (http://www.ensambl.org/) Human 13,31 Database and Human Genome Sequencing Project and directly deals with alternative splicing variants using common, partial common, or individual transcript oligos. For the probe design we use state-of-the-art methodology and proprietary software. An amino linker is attached to the 5`end of each oligo. http://www.operon.com/arrays/oligosets_human.php. The 34,580 70mer probes were dissolved in 30% DMSO to a concentration of 18 µM. The microarrays were printed with a QArray2 (Genetix) instrument with 48 K2805 pins (Genetix) on Ultra GAPS slides (Corning). The 34,580 longmer probes were spotted in a 28x28 pattern within each block and with a feature center-to-center distance of 155 µm. The quality of the spotted slides was assessed by staining with Syto61 (Molecular Probes) and with hybridization with random nonamers. The slides were UV cross-linked at 150 mJ/cm2.