title: Schistosoma japonicum 12 x 135k microarray
status: Public on Jul 19 2015
contact.city: Beijing
contact.first: Shuai
contact.last: Liu
contact.address: Dong Dan San Tiao 9, Beijing, China
contact.country: China
contact.institute: Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College
organism: Schistosoma japonicum
description: The platform was designed using the sequences obtained from schistosome genome and UniGene database  on NCBI by NimbleGen. A total of 21,861 target sequences (20,194 sequences derived from S. japonicum and 1,667 sequences derived from S. mansoni) were provided for array design, for each of the sequences, 3 or 4 unique 60-mer oligonucleotide probes were designed. There were 131,092 experimental probes corresponding to both strands of the genomic DNA on the microarray. Besides the experimental probes, control probes were printed on the array. Probes with random sequences were printed as negative control and eight spike-RNA probes which from the intergenic sequence of yeast as positive controls. The microarray was manufactured by NimbleGen.
  The native array description file  and  schistosome sequence file used for arrray design are linked below as supplementary files.
  110218_Sjaponicum_YR_exp_2.ndf
manufacture_protocol: See manufacturer's website at http://www.nimblegen.com/.