title: M20K (Incyte mouse release 1.0 and Unigene1)
status: Public on May 15 2003
contact.city: Memphis
contact.first: Deanna
contact.middle: Cole
contact.last: Naeve
contact.email: deanna.naeve@stjude.org
contact.state: TN
contact.address: 332 N. Lauderdale
contact.department: Hartwell Center for Bioinformatics and Biotechnology
contact.country: USA
contact.institute: St. Jude Children's Research Hospital
contact.phone: 901-495-4847
organism: Mus musculus
description: The M20K microarray includes 18,330 cDNA mouse clones from the Incyte mouse release 1.0 and Unigene 1 collections. The PCR amplified cDNA inserts were either obtained from Incyte or were amplified from replica plate cultures, purified and mechanically spotted onto polylysine-coated microscope slides using an OmniGrid robotic spotter (GeneMachines, San Carlo, CA).  Printed slides were post-processed according to the procedures on the Stanford website with some modifications: http://cmgm.stanford.edu/pbrown/protocols/index.html.