title: NCI/ATC Mm-MEEBO
status: Public on Jan 01 2019
contact.city: Houston
contact.first: Sangbae
contact.last: Kim
contact.email: sangbkim22@gmail.com
contact.state: TX
contact.address: 1 Baylor plaza
contact.department: HGSC
contact.country: USA
contact.institute: Baylor Colleage of Medicine
organism: Mus musculus
manufacture_protocol: Microarrays are printed with GeneMachines robotic arrayer from Genomic Solutions (http://www.genomicsolutions.com) that is fitted with a printhead containing 48 SMP2.5 printing pins from Telechem International (http://www.arrayit.com). The mouse long oligonuleotide probe set contains 38,517 optimized 70mers (plus NCI Spotplate #4 controls) obtained from Invitrogen (http://www.invitrogen.com/). The long oligonucleotide probes were purchased in 384-well polypropylene plates (Genetix, 384 microarray) and dissolved print buffer of sodium phosphate (150mM) at ph 8.5 and 4% sodium sulphate at a concentration of 20-30 uM. The microarray spots are printed with 160 micron column x 155 microns row spacing, and one spot is printed per probe per slide. The microarrays were printed on Corning Expoxide slides and post-processed according to the manufacturer's protocol.