title: UTSW Human 22k Oligo spotted array
status: Public on Aug 19 2004
contact.city: Stillwater
contact.first: Patricia
contact.middle: J
contact.last: Ayoubi
contact.email: ayoubi@okstate.edu
contact.state: OK
contact.address: 246 NRC
contact.department: Biochemistry and Molecular Biology
contact.country: USA
contact.web_link: http://microarray.okstate.edu
contact.institute: Oklahoma State University
contact.phone: 405-744-6209
organism: Homo sapiens
description: Construction and Preparation of Human Oligo 22K Spotted Microarrays by UTSW:  Operon Human Genome Oligo Set (Version 2) (70mers) representing 21,329 human genes in 150mM Sodium Phosphate (pH8.3) plus 1xSSC at 30 µM were spotted singly onto polymer-coated glass slides (Full Moon Biosystems) using BioRobotics MicroGrid II spotter (Genomic Solutions) fitted with 4x12 Microspot 2500 quill pins at 20-23°C and 60-65% RH.  Spot are arranged with 22x22 spots per block (48 total blocks) and spot centers are 200 µm apart.  Printed slides were subjected to a UV-cross-linking at a dose of 650mJ cm2 for probe immobilization. Slides with immobilized probes were then stored in slide holders and placed in a desiccator at room temperature.