title: University of Padova Anguilla anguilla 8x15K v1.0
status: Public on May 22 2013
contact.city: Legnaro
contact.first: Massimo
contact.last: Milan
contact.email: massimo.milan@unipd.it
contact.state: PD
contact.address: Viale dell'Università 16
contact.department: Dept. of Department of Public Health, Comparative Pathology,and Veterinary Hygiene
contact.country: Italy
contact.institute: University of Padova
contact.phone: +39 0498272941
organism: Anguilla anguilla
description: Target sequences to be included in the European eel microarray were obtained from the assembly of a total of 640,040 sequence reads obtained by 454 sequencing. The array included all annotated sequences with a blast hit to vertebrates and invertebrates but excluding bacteria, virus, plants, fungi and protozoa. For 12,296 contigs, the putative orientation was unambiguous and a single probe was designed. For 1,390 contigs, the putative orientation was ambiguous and two probes were designed. In order to minimize assembler redundancy, 163 contigs differing by less than 3 nucleotide changes were discarded. The final number of probes was 14,913. Probe design was carried out using Agilent eArray Interface which applies proprietary prediction algorithms to design 60-mer oligoprobes. Microarrays were synthesized in situ using the Agilent ink-jet technology with an 8x15K format (8 arrays in a single slide). Each array contained default positive and negative controls.
manufacture_protocol: See SurePrint Technology at manufacturer's web site http://agilent.com/