title: VTT_KB_Aspergillus nidulans v4.0_135K array
status: Public on Apr 16 2012
contact.city: Espoo
contact.first: Kirsi
contact.last: Bromann
contact.email: kirsi.bromann@vtt.fi
contact.address: Tietotie 2
contact.department: TK402, Cell Factory
contact.country: Finland
contact.institute: VTT Technical Research Centre of Finland
organism: Aspergillus nidulans FGSC A4
description: Nimblegen design name 100608_Anid_KB_exp, Nimblegen design ID 531573. Expression + Tiling design for Aspergillus nidulans FGSC A4 using customer-provided transcript set. Expression portion -- selected 6 probes per transcript for 10546 out of 10597 transcripts (51 not found, 18 less than 6 probes per transcript, 114 duplicate probes/exemplars).  2 replicates of 63,130 probes for a final expression total of 126,260 probes. Tiling portion -- Used customer provided list of 36 long transcripts.  Selected 5651 tiling 50-75mers and tiled at a 66bp interval (int=56/win=10/mm=1). 2 replicates on final array for 11302 tiling probes.  Created .pos and probe_locations.gff to go with tiling portion.  Annotation for these regions can be found in the .ngd file
manufacture_protocol: High-density DNA array prepared with Maskless Array Synthesizer (MAS) technology. See manufacturer's website at http://www.nimblegen.com/.