title: RIKEN Mouse Neues microarray version 2
status: Public on Feb 09 2007
contact.city: Hidaka
contact.first: Itoshi
contact.last: NIKAIDO
contact.email: itoshi@saitama-med.ac.jp
contact.state: Saitama
contact.address: 1397-1 Yamane
contact.country: Japan
contact.institute: Saitama Medical School
organism: Mus musculus
description: RIKEN Mouse Neues Microarray v2.0 contains 21,943 60-mer oligonucleotides representing over 20,000 known and predicted mouse genes. The sequence is based on the RIKEN cDNA clone sequences and public databases (RefSeq, Ensembl).
manufacture_protocol: Validated probes are probes optimized using a novel experimental method for the selection and validation of highly specific oligonucleotide microarray probes. This probe optimization strategy is based on the assumption that, if multiple probes for a particular gene are specific for that gene, they will show similar differential expression behavior. Optimized 60-mer probes are selected from clusters of computationally determined candidate probes that perform similarly across a number of differential gene expression experiments. This method also enables verification of computationally predicted gene assemblies. These selected probes are then experimentally validated based on hybridization responses to all known genes for which they were designed.
  
  Agilent uses its inkjet technology to print oligos and whole cDNAs onto glass slides. The non-contact inkjet technology produces microarrays with more uniform and consistent features. The inkjet technology is used as part of an industrial-scale manufacturing process that enables us to reliably produce uniform, high quality microarrays from lot to lot. Since the inkjet process is a non-contact process, it does not introduce defects as a result of surface tension interactions with the microarray surface.