title: Kanazawa Univ. Human Liver chip 10k
status: Public on Aug 19 2012
contact.city: Kanazawa
contact.first: Masao
contact.last: Honda
contact.email: mhondag@gmail.com
contact.address: Takara-Machi 13-1
contact.department: Gastroenterology
contact.country: Japan
contact.institute: Kanazawa University
contact.phone: 0762652243
organism: Homo sapiens
description: In addition to in-house cDNA microarray slides consisting 1080 cDNA clones as previously described（Gastroenterology. 2001 Mar;120(4):955-66, we made a new cDNA microarray slide for detailed analysis of the signaling pathway of metabolism and enzyme function in liver disease. Besides cDNA microarray analysis, a total of 256 550 tags were obtained from hepatic SAGE libraries (derived from normal liver, CH-C, CH-C related HCC, CH-B, CH-B related HCC respectively), including 52 149 unique tags. Among these, 16 916 tags expressing more than two hits were selected to avoid the effect of sequencing errors in the libraries. From these candidate genes, 9,614 non-redundant clones were obtained from Incyte Genomics (Incyte Corporation, Beverly, MA, USA), Clontech (Nippon Becton Dickinson, Tokyo, Japan), and Invitrogen (Invitrogen Japan K.K., Tokyo, Japan). Each clone was sequence validated and PCR amplified by Dragon Genomics (Takara Bio, Otsu, Japan), and the cDNA microarray slides (Liver chip 10k) were constructed using SPBIO 2000 (Hitachi Software, Fukuoka, Japan) as described previously.
manufacture_protocol: see manufacturer's website