title: Campylobacter jejuni 2K whole-ORF array v1.2
status: Public on Jul 08 2009
contact.city: East Lansing
contact.first: Linda
contact.middle: S.
contact.last: Mansfield
contact.email: mansfie4@cvm.msu.edu
contact.state: MI
contact.address: 181 Food Safety Bldg.
contact.department: Microbiology and Molecular Genetics
contact.country: USA
contact.institute: Michigan State University
contact.phone: 517-884-2027
organism: Campylobacter jejuni
description: Array includes ORF coverage from array "Campylobacter jejuni 6.5K whole-ORF array v1.0" (GPL7254) plus an additional 46 ORFs amplified from strain 11168 using primers designed at Michigan State University.
manufacture_protocol: 1.) Primers were designed at Wayne State University by Jodi R. Parrish, furnished to Michigan State University, and are described in “High-Throughput Cloning of Campylobacter jejuni ORFs by in Vivo Recombination in Escherichia coli”, Journal of Proteome Research, Parrish et al., 2004 3(3):582-6.  Additional primers were designed at Michigan State University to amplify 46 additional ORFs or putative ORFs from strain 11168.
  2.) PCR products were amplified from genomic DNA from Campylobacter jejuni 11168 or plasmid DNA obtained from C. jejuni 81-176. Products were ethanol precipitated, resuspended in 3X SSC, 0.005% SDS print buffer, and run on gels for quality control.
  3.) 16 pin replicate arrays were printed on Corning UltraGAPS slides using a GenMachines Omnigrid spotting robot and Telechem Chipmaker 3 pins.
  4.) Each slide contains every spot once.