title: Ocimum Biosolutions E.coli K12 V2 OciChip
status: Public on Apr 07 2010
contact.city: Hyderabad
contact.first: Ocimum
contact.last: Biosolutions
contact.email: govind.a@ocimumbio.com, sandhya.p@ocimumbio.com, hazira.b@ocimumbio.com, anwaruddin.m@ocimumbio.com
contact.state: Andhra Pradesh
contact.address: Banjara Hills Road No., 1 VI Floor Reliance Classic
contact.department: Bioinformatics
contact.country: India
contact.institute: Ocimum Biosolutions
organism: Escherichia coli K-12
manufacture_protocol: E.coli K12 V2 Ocichip consists of 4608 spots printed on Corning® epoxy coated glass slides using Omnigrid (Gene Machines). Chip has been printed using 4x4 SMP4 pins on 75.5x 25.5x1.3 mm microarray glass slides with a spot area of 18x18 cm. The origin offset values were 0 for X direction and 10000 for Y direction. The dot to dot distance is 260 mm; distance between subarrays is 150 mm and no of dots per array is 16x18=288. Oligos were printed using spotting buffer A from Ocimum Biosolution at 50% humidity and temperature at 22°C. The time taken by the robotic gridder to print was 16 hrs. Each probe was checked for its performance by standard QC process followed at Ocimum Biosolutions. Printed slides were stored in a light-tight box in a bench-top dessicator, with desiccant and were kept at 4°C until they were used for hybridization.