title: MSU_Rose_Env_Vir_v3
status: Public on Feb 28 2008
contact.city: East Lansing
contact.first: Mark
contact.last: Wong
contact.state: MI
contact.address: 3 Natural Resources
contact.department: Crops and Soil Sciences
contact.country: USA
contact.institute: Rose_Lab@MSU
organism: Viruses
manufacture_protocol: Oligonucleotide probes are synthesized in-situ using conventional DMT-protected phosphoramidite chemistry. A photogenerated acid (PGA) is used instead of the conventional acid DMT deprotection step. In photogenerated acid deprotection, deprotection is initiated by directing light at selected three-dimensional chambers in microfluidic chips. Upon activation, acid forms in seconds, removing DMT group.
  An incoming phosphoramidite nucleoside monomer is then coupled to the growing oligomer chain. The synthesis cycle is repeated for each additional monomer until an array of thousands of oligonucleotides is formed.