title: High-throughput pyrosequencing of small RNAs in Tomato fruits and leaves
status: Public on Jul 21 2008
contact.city: Norwich
contact.first: Frank
contact.last: Schwach
contact.email: f.schwach@uea.ac.uk
contact.address: University of East Anglia
contact.department: School of Computing Sciences
contact.country: United Kingdom
contact.institute: University of East Anglia
organism: Solanum lycopersicum
description: The dataset contains Lycopersicon esculentum cv. MicroTom endogenous small RNA sequences in the range 16-30 nucleotides. High-throughput sequencing was carried out at 454 Life Sciences. Please see www.454.com for details of the technology. Total RNA was extracted from tomato leaf, bud before flower blooming and different developmental stages of whole fruits.Small RNA sequences that did not match tRNA or rRNA sequences (obtained from RFAM), The Genomic tRNA Database and EMBL were mapped to bacs version 175 of the Solanum lycopersicum cv. Heinz 1706-BG genome.  The number of matches to the unfinished genome, if any, is recorded in the table.
manufacture_protocol: see manufacturer website www.454.com