title: Udall global cotton16 EST assembly array
status: Public on Sep 11 2008
contact.city: Athens
contact.first: Alan
contact.middle: R
contact.last: Gingle
contact.email: agingle@uga.edu
contact.state: GA
contact.address: 111 Riverbend Rd.
contact.department: Center for Applied Genetics Technologies
contact.country: USA
contact.institute: University of Georgia
organism: Gossypium
description: We have designed and implemented a novel NimbleGen microarray platform capable of measuring global gene expression in Gossypium species. This custom designed microarray features ~60mer generic probes those were created by first assembling EST contigs from G.arboreum, G.raimondii, and G. hirsutum libraries and has the ability to measure total gene expression.
manufacture_protocol: Oligonucleotide arrays were synthesized by NimbleGen Systems, Inc by maskless array synthesis using a digital micromirror array-mediated,parallel synthesis process incorporating 5'-photoprotected phosphoramidites. Synthesized arrays were deprotected by incubation in ethanolamine:ethanol (1:1) at room temperature for two hours, washed extensively in distilled water, and stored desiccated until used. This custom design has 42,460 cotton genes and includes ~290,000 expression probes.