title: Bowen BC016 oligo array
status: Public on Jan 13 2018
contact.city: Nahant
contact.first: Jennifer
contact.last: Bowen
contact.email: je.bowen@northeastern.edu
contact.state: Massachusetts
contact.address: 430 Nahant Rd.
contact.department: Marine and Environmental Sciences
contact.country: USA
contact.institute: Northeastern University
organism: uncultured bacterium
manufacture_protocol: 1.The probe oligonucleotides are printed onto Corning Ultragaps or Erie Scientific Epoxy Silane slides using a DeRisi style arrayer (DiRisi et al., 1997) and pins from Parallel Synthesis.
  2.The 70-mer oligo probes (Operon Technologies) were adjusted to a concentration of 0.05 mg ml-1 in 50%  DMSO and were spotted on CMT-GAPS amino silane-coated  glass slides (Corning, Corning, NY).
  3. After printing, the slides were stored in the dark at room temperature under slight vacuum in a dessicator. They were UV irradiated before hybridization,