title: Pangenomic Ostreococcus microarray (8K)
status: Public on Apr 20 2010
contact.city: Rennes
contact.first: annabelle
contact.last: monnier
contact.email: annabelle.monnier@univ-rennes1.fr
contact.address: 2 avenue du Professeur Léon Bernard
contact.country: France
contact.web_link: http://www.umr6061.univ-rennes1.fr
contact.institute: UMR 6061 Génétique et Développement
contact.phone: +33 (0)2 23 23 61 14
organism: Ostreococcus tauri
manufacture_protocol: 1. 8096 gene-specific oligonucleotides (50-mers) were designed and synthesized by Eurogentec.
  2. Oligonucleotides were resuspended at a 30 microM concentration in Nexterion Spot Solution 2X (Schott Nexterion).
  3. Oligonucleotides were spotted in triplicate on Nexterion Slides E (Schott) using a Biorobotics MicroGrid II spotter (Genomic Solutions).  24k feature array comprises technical triplicates.
  4. Positives (Cy3 labelled oligonucleotides) and negatives (spotting buffer) controls were added
  5. Quality of spotting was checked by slide scanning using an Agilent G2565BA Microarray scanner (Agilent Technologies).