title: Apoptosis and Survival Tier 1-4, H384
status: Public on Feb 01 2026
contact.city: Amsterdam
contact.first: Neeltje
contact.last: Kootstra
contact.email: n.a.kootstra@amsterdamumc.nl
contact.address: Meibergdreef 9
contact.country: Netherlands
contact.institute: AmsterdamUMC
organism: Homo sapiens
description: PCR assay
manufacture_protocol: see manufacturer's website; PCR arrays were using the 384 well plates Apoptosis and Survival Tier 1-4 from Bio-Rad (Hercules, CA, USA), where 384 primer pairs are lyophilized on the plate. 10 ng cDNA was used for qPCR with GoTaq® qPCR Master Mix (Promega, Madison, WI, USA) on the LightCycler 480 System (Roche, Basel, Switzerland). The following cycling conditions were used: denaturation: 95 °C for 2 min; amplification: 40 cycles of 95°C for 5 sec and 60°C for 30 sec, followed by a melting curve stage.