title: Agilent-063245 E. coli Custom 4 X 44K array (Probe name version)
status: Public on Oct 10 2016
contact.city: Seoul
contact.first: Ji-Min
contact.last: Woo
contact.email: wjm87ewhain@naver.com
contact.address: Ewhayeodae-gil
contact.country: South Korea
contact.institute: Ewha womans university
organism: Escherichia coli
manufacture_protocol: Microarray interrogations were performed using a custom-designed, Agilent-based microarray platform with 4 × 44 K probes per slide (E.coli_chip; Agilent Design ID:063245). Total of 3936 coding sequences of K12 and 4007 coding sequences of BL21 were obtained from NCBI Database and 1 probe was designed for each coding sequence using eArray software according to standard Agilent probe design criteria. We successfully designed 60-mer probes for 7,943  using eArray software (http://earray.chem.agilent.com/earray/) and microarrays were manufactured using Agilent Technologies (USA).