title: EcoArray 1 x 22K Fathead Minnow Microarray (v1 and v2)
status: Public on Sep 20 2008
contact.city: Alachua
contact.first: Barbara
contact.middle: Joan
contact.last: Carter
contact.email: bcarter@appliedfoodtechnologies.com
contact.state: FL
contact.address: 12085 Research Drive
contact.department: R&D
contact.country: USA
contact.institute: Applied Food Technologies
organism: Pimephales promelas
description: This second generation array has approximately 22,000 fully-annotated genes; 90% of these genes have been matched (e-score < E-04) to named genes in the published databases. The genes are part of a wide variety of biological pathways and processes including: reproduction, cell communication, metabolism, homeostasis, and others.  The full list of genes is on our website: www.ecoarray.com.  These arrays are low-density (10 um scanner) and are no longer produced by Agilent.  They have been replaced by our higher density FHM 4 x 44K and the 8 x 15K arrays.
manufacture_protocol: See manufacturer's web site at http://www.agilent.com/. These genes are spotted onto microarrays created for EcoArray by Agilent Technologies, a leading supplier of genomics and proteomics products.  Agilent uses their SurePrint inkjet deposition of in situ synthesized 60-mer oligonucleotides, to manufacture these chips on superior quality 1” x 3” (24.2 mm x 76.2 mm) treated glass slides for overall excellent performance.  This process minimizes variability across each production lot (batch to batch) and provides the highest consistency in slide-to-slide comparisons.  These glass slide microarrays are designed for either a one- or two-color (cyanine-3 and cyanine-5) labeling reaction, the most reliable and sensitive method for detecting differentially expressed genes.  Each individual microarray produces QA metrics to ensure quality.