title: Barley_array_v35-01
status: Public on Apr 29 2009
contact.city: Erlangen
contact.first: Weber
contact.last: Simone
contact.email: simoneweber.fr@gmail.com, Selina.Sitte@uk-erlangen.de
contact.state: Bavaria
contact.address: Ulmenweg 18
contact.department: Medicine
contact.country: Germany
contact.institute: Friedrich-Alexander University Erlangen-Nuremberg
contact.phone: 0049-9131-8535000
organism: Hordeum vulgare
description: Product: Number G2519F
  Design ID: 017060
  Slide Format: 4 x 44K
  Features / Microarray: 440000  Genes / Microarray: ~40,000 (41.196)
  Content Source: Harvest database
  Manufacturing Process: In situ oligo synthesis
  Slide Dimensions: 1 x 3" (25 x 75 mm)
  Probe Type: In situ
  Probe Length: 60 bases
  Probe Orientation: Sense
  Feature Size: 4 x 44K = ~65 microns
  Based on sequence information of 444.652 ESTs (february 2007; http://harvest-web.org/) a 44K 60mer Agilent oligoarray was designed. To this end all 28.001 consensus sequences and ca. 13.000 selected singletons were chosen to derive a specific set of oligos using the web-based earray-tool from Agilent (http://earray.chem.agilent.com/earray/). The respective Singletons were considered if they had a significant blast hit in rice and/or A. thaliana (E-Value >-10). After verifying the quality of the oligos barley -oligoarray (gerste_array_v35-01) was created comprising 41196 60mers. 2607 genes were randomly selected and spotted twice.
manufacture_protocol: See the manufacturer's website
  http://www.agilent.com/