title: Hofmann A. burtoni 16K brain_pinky_array_print4
status: Public on Jan 25 2008
contact.city: Portland
contact.first: Suzy
contact.middle: C.P.
contact.last: Renn
contact.email: renns@reed.edu
contact.state: OR
contact.address: 3203 SE Woodstock Blvd
contact.department: Biology
contact.country: USA
contact.institute: Reed College
contact.phone: 503 517 7967
organism: Haplochromis burtoni
description: synonym: Astatotilapia burtoni
  the array consists of 48 blocks (4#12) of 400 spots each (20X20)
  the physical array contains an adition set of yeast control features that are not included in the GEO platform
  NO EMPTY FEATURES NOR UNRELIABLE SPOTS (BAD PCR PRODUCT) ARE INCLUDED IN THIS PLATFORM
manufacture_protocol: This array is built primarily from two Cichlid cDNA libraries. Library 1: Brain2000 was made in the lab of Russ Fernald using brains from all sexes, stages, and phenotypes. Library 2: Pinky2003 was made by Walter Salzburger using mixed tissues including gonads, liver, skin, muschle from all stages. this library was normalized Both libraries represent Astatotilapia burtoni Colony picking, PCR amplification, and array printing have been done at Harvard's Bauer Center for Genomics Research in the lab of Hans Hofmann. the array also includes candidate genes cloned in the Hofmann and Fernald labs as well as yeast control genes and a few other positive and negative controls