title: DaphniaArray
status: Public on Dec 26 2005
contact.city: Okazaki
contact.first: Hajime
contact.last: Watanabe
contact.email: watanabe@nibb.ac.jp
contact.state: Aichi
contact.address: 5-1, Higashiyama
contact.department: Center for Integrative Bioscience
contact.country: Japan
contact.institute: National Institutes of Natural Sciences
contact.phone: 81-564-59-5237
organism: Daphnia magna
manufacture_protocol: Based on EST database of daphnia magna (Daphniabase, http://daphnia.nibb.ac.jp), 178 genes were selected for DNA microarray.  Genes were selected based on their annotations.  Genes that have similarities to other genes related to detoxification and metabolism were mainly selected.  Other EST genes were randomly selected from the database.
  An oligonucleotid corresponding to each gene was designed so that it did not have similarities to other sequences in the database.  Then the oligonucleotids were synthesized and spotted onto Geneslide (Toyokohan, Tokyo, Japan), whose surface is coated with carbon that facilitates covalent linkage of DNA.  Spotting was performed by manufacturer (NGK Insulators, Nagoya, Japan) by ink-jet printer.