title: IFTC Rattus norvegicus 1152
status: Public on Feb 24 2009
contact.city: Hannover
contact.first: Cornelia
contact.last: Repenning
contact.address: Callinstr 5
contact.country: Germany
contact.institute: Institut fuer Technische Chemie
organism: Rattus norvegicus
manufacture_protocol: Oligonucleotide design and synthesis: 50mer oligonucleotides with a 5' aminolink were designed and synthesised by MWG (Ebersberg, Germany). Spotting protocol: To produce microarrays silylated slides were purchased from TeleChemTM International Inc. (Sunnyvale, CA, USA) and the printing of the oligonucleotides was performed by using an AffymetrixTM 417 arrayer. Oligonucleotide solutions were prepared in 96-well-plates containing each 30 µl. The concentration of the printed oligonucleotides was 50 µM in 1x ArrayItTM Microarray Spotting Solution Plus. After spotting, the slides were incubated at 80 °C for 2 h. Slides were blocked in SSC containing 1% BSA (Sigma) for 45 min and rinsed with water for 5 min.