title: Duke Taeniopygia guttata 45K
status: Public on Jan 16 2010
contact.city: Durham
contact.first: Osceola
contact.last: Whitney
contact.email: whitney@neuro.duke.edu
contact.state: NC
contact.address: Research Drive, Bryan Research Building, Room 401E
contact.department: Neurobiology
contact.country: USA
contact.web_link: http://songbirdtranscriptome.net/
contact.institute: Duke University Medical Center
contact.phone: 919-681-1681
organism: Taeniopygia guttata
manufacture_protocol: Using Agilent eArray 5.4, a web-based application, we designed custom microarrays. The microarrays are manufactured using a proprietary non-contact industrial inkjet printing process, in which oligo monomers are deposited uniformly onto specially-prepared glass slides. This in situ synthesis process prints 60-mer length oligonucleotide probes, base-by-base, from digital sequence files. The precise inkjet process enables the delivery of extremely small, accurate volumes (picoliters) of the chemicals to be spotted. Standard phosphoramidite chemistry used in the reactions allows for very high coupling efficiencies to be maintained at each step in the synthesis of the oligonucleotide. Agilent’s real time quality control inspection system verifies chemical deposition at each step in the process to minimize or eliminate feature drop-out and premature truncation of the oligonucleotide probe.