title: CEA/UWash Yeast High Density ORF array
status: Public on Jun 14 2007
contact.city: Seattle
contact.first: Kenneth
contact.middle: M
contact.last: Dombek
contact.email: kmd@uw.edu
contact.state: WA
contact.address: 1959 Pacific St. N.E.
contact.department: Biochemistry
contact.country: USA
contact.institute: University of Washington
contact.phone: 206-616-9259
organism: Saccharomyces cerevisiae
manufacture_protocol: 1.  Each yeast ORF sequence was amplified from S288C genomic DNA by PCR. The forward primer sequence begins at the start codon and the reverse primer sequence begins at the stop codon of each ORF. The resulting PCR products were full-length ORF DNAs.
  2.  PCR products were purified, dried, suspended in 50% DMSO, and deposited on 75- by 25-mm type VII coated glass microscope slides from AP Biotech using a Molecular Dynamics Generation III microarray spotter (Sunnyvale, CA).
  3.  After the spotting, the microarrays were air dried, cross-linked at 450 mJ, and stored dessicated under nitrogen until needed.