title: ACRI_Human2_35K [full layout version]
status: Public on Apr 18 2011
contact.city: Halifax
contact.first: Jason
contact.last: Williams
contact.email: jason.williams@nrc-cnrc.gc.ca
contact.state: Nova Scotia
contact.address: 1411 Oxford Street
contact.department: Institute for Marine Biosciences
contact.country: Canada
contact.institute: National Research Council
contact.phone: (902)426-5903
organism: Homo sapiens
manufacture_protocol: Human DNA probes were synthesized at the Atlantic Microarray Facility (Moncton, NB, Canada) by the phosphoramidite method at a 40 nmole scale on a Gene Machines PolyPlex 2 oligonucleotide synthesizer (Genomic Soutions, Ann Arbor, MI, USA). Isolated DNA was analyzed by ESIElectrospray ionisation-Mass Spectrometry using an Agilent VL1100MSD mass spectrometer (Agilent Technologies, Inc., Mississauga, ON, Canada). DNA of low quality was re-synthesized. The human probes spotted in side –by-side duplicate on epoxide microarray slides (Schott) to give 34944-feature microarrays. An OmniGrid 100 microarrayer (Genomic Solutions) equipped with SMT-S50 silicon print pins (Parallel Synthesis Technology, Santa Clara, CA, USA) was used.
  Spotted microarrays were analyzed by SpotQC (IDT Inc., Coralville, IA, USA) for any defects in spotting that may have occurred. Microarrays were stored at room temperature in vacuum-sealed microarray pouches (Corning Inc., Corning, NY, USA).