title: Meningococcal antigens microarray
status: Public on Mar 02 2026
contact.city: Manchester
contact.first: Fidel
contact.last: Ramirez Bencomo
contact.email: fidel.ramirezbencomo@manchester.ac.uk
contact.state: England
contact.address: Michael Smith Building
contact.department: FBMH/SBS/EIGen
contact.country: United Kingdom
contact.institute: The University of Manchester
organism: Neisseria meningitidis
manufacture_protocol: Recombinant proteins were prepared at 0.5 mg/mL in 40% glycerol. Positive controls consisted of 8 serial 2-fold dilutions of mouse, human, rat, and rabbit IgG (from 0.5 mg/mL to 0.004 mg/mL in 40% glycerol). Negative controls were the different protein buffers + 40% glycerol. All samples were printed randomly in replicates (four times) onto ultra-thin nitrocellulose-coated glass slides (FAST slides; Maine Manufacturing). Printing was performed with the ink-jet spotter Marathon Argus (Arrayjet, UK) (200 pL for each spot) in a cabinet with controlled temperature and humidity (18°C and 50-55%, respectively). The efficiency and the reproducibility of protein immobilisation were assessed by probing some test slides with mouse anti-His tag monoclonal antibodies (Sigma Aldrich, SAB1305538) diluted 1:1000 and followed by detection with an AlexaFluor 647-conjugated anti-mouse IgG secondary antibody (Jackson Immunoresearch) diluted 1:800