title: Agilent-031272 Aspergillus nidulans expression array V5 (Feature Number version)
status: Public on Sep 01 2013
contact.city: Kgs. Lyngby
contact.first: Mikael
contact.middle: R
contact.last: Andersen
contact.email: mr@bio.dtu.dk
contact.address: Søltofts plads, bygn 223
contact.department: BioCentrum-DTU
contact.country: Denmark
contact.institute: Technical University of Denmark
organism: Aspergillus nidulans FGSC A4
description: DTU-Anidulans-44K-V5
  4x44K
  
  Arrays of this design have barcodes that begin with 16031272 or 2531272.
  
  Orientation:
  Features are numbered numbered Left-to-Right, Top-to-Bottom as scanned by an Agilent scanner (barcode on the left, DNA on the back surface, scanned through the glass), matching the FeatureNum output from Agilent's Feature Extraction software.
  
  The ID column represents the Agilent Feature Extraction feature number.
  
  Rows and columns are numbered as scanned by an Axon Scanner (barcode on the bottom, DNA on the front surface).
  
  To match data scanned on an Axon scanner, use the RefNumber column contained in the Agilent-provided GAL file as the ID_REF column in sample submissions.
manufacture_protocol: Probes designed using the OligoWiz 2.0 software (Workman et al., 2002) from the CDS sequences of predicted ORFs from the genome sequence of A. nidulans FGSC A4 using version 5 of the A. nidulans gene annotation, downloaded from the Aspergillus Genome Database (aspgd.org). For each gene, a maximum of 3 non-overlapping perfect-match 60-mer probes were calculated by using the OligoWiz standard scoring of cross-hybridization, melting temperature, folding, position preference, and low complexity. A position preference for the probes was included in the computations. Pruning of the probe sequences was done by removing duplicate probe sequences. The arrays were manufactured according to the current standards for Agilent 4x44K arrays.