title: NIAID Ssca -- Strongyloides stercoralis 4K - mAdb version
status: Public on Oct 27 2010
contact.city: Bethesda
contact.first: Timothy
contact.middle: G
contact.last: Myers
contact.email: tgm@nih.gov
contact.state: MD
contact.address: 50 South Drive, Room 5509
contact.department: Research Technologies Branch
contact.country: USA
contact.institute: National Institute of Allergy and Infectious Diseases
organism: Strongyloides stercoralis
description: Oligonucleotide probes were designed to hybridize 3,572 contigs generated by the Strongyloides sequencing project. Microarrays were manufactured by Agilent SurePrint(TM) to produce spotted glass slide arrays. The spot density was 22K spots per array. Of the 6 oligos designed per target, 1 was designed using the Array Designer program from Premier Biosoft, 2 were designed using using E-Array (Agilent) using "base composition" method) (replicated twice), 2 were designed using E-array "best Tm" method, the last one was a 40-mer designed using Array Designer. Probes were selected to avoid cross-hybridization to other sequences in the target (contig) dataset.
  Agilent designID: 015083
manufacture_protocol: see manufacturer's web site at http://www.agilent.com/