title: SLRI_Zebrafish_3.5k_v1
status: Public on Mar 16 2006
contact.city: Toronto
contact.first: Chi-Yip
contact.last: Ho
contact.email: cyho@mshri.on.ca
contact.state: Ontario
contact.address: 600 University Ave. rm-981
contact.department: Research
contact.country: Canada
contact.web_link: http://www.mshri.on.ca/microarray
contact.institute: SLRI/Mount Sinai Hospital
contact.phone: 416-586-1589
organism: Danio rerio
manufacture_protocol: This microarray is printed in triplicate with Operon's the Zebrafish Genome Oligo Set Version 1.0 contains 3,479 70mer probes representing 3,479 Danio rerio genes. The set of 3,479 probes represents cloned Zebrafish genes and some expressed sequence tags (ESTs). For our probe design we use state-of-the-art methodology and proprietary software. All probes are designed from the UniGene Database Build Dr 41, and the Zebrafish Reference Sequence (RefSeq) database, developed and maintained at the National Center of Biotechnology Information (NCBI) (http://www.ncbi.nlm.nih.gov). For the oligo set design, we selected 1,206 sequences, representing well-annotated genes, and 2,273 ESTs with high or moderate homology to known genes. The probes are Tm normalized to 78ºC (±5ºC) and have a 5' amino linker. The concentration of all probes are normalized to 40 micromolar and printed on NUNC poly-L-lysine coated slides using BioRad VersArray Chipwriter Pro.