title: NCI/ATC Mm-MEEBO 38K
status: Public on Sep 28 2011
contact.city: Bethesda
contact.first: Daekwan
contact.last: Seo
contact.email: daekwan_seo@nih.gov
contact.state: MD
contact.address: 37 Convent Dr. Rm 4140
contact.department: NCI
contact.country: USA
contact.institute: NIH
contact.phone: 301-496-5688
organism: Mus musculus
description: NCBI mouse genome Build 37 (UCSC mm9).
  Derived from GPL4200.
manufacture_protocol: Microarrays are printed with GeneMachines robotic arrayer from Genomic Solutions (http://www.genomicsolutions.com) that is fitted with a printhead containing 48 SMP2.5 printing pins from Telechem International (http://www.arrayit.com). The mouse long oligonuleotide probe set contains 38,467 optimized 70mers (plus NCI Spotplate #4 controls) obtained from Invitrogen (http://www.invitrogen.com/). The long oligonucleotide probes were purchased in 384-well polypropylene plates (Genetix, 384 microarray) and dissolved print buffer of sodium phosphate (150mM) at ph 8.5 and 4% sodium sulphate at a concentration of 20-30 uM. The microarray spots are printed with 160 micron column x 155 microns row spacing, and one spot is printed per probe per slide. The microarrays were printed on Corning Expoxide slides and post-processed according to the manufacturer's protocol.