title: Lactococcus lactis IL1403 and lactococcal plasmids 2.5K
status: Public on Aug 31 2013
contact.city: Tsukuba
contact.first: Miho
contact.last: Kobayashi
contact.email: mihokoba@affrc.go.jp
contact.address: 2 Ikenodai
contact.department: Animal Products Research
contact.country: Japan
contact.institute: National Institute of Livestock and Grassland Science
contact.phone: +81-29-838-8685
organism: Lactococcus lactis
description: The genome sequences and ORF predictions for the chromosome of L. lactis IL1403 and several lactococcal plasmids were obtained from GenBank (accession numbers J05748, AF247159, X99798, AF409136, AF242367, AF036485, AF243383, AF207855, J04962, and AE005176). The genome sequences encoded a total of 2533 predicted ORFs (2425 genes from IL1403 and 108 genes from plasmids). The custom oligonucleotide microarrays contained 111,452 probe pairs, with each pair containing a perfect-match 24-mer oligonucleotide probe. Microarray hybridization experiments were performed at the GeneFrontier Corporation (Tokyo, Japan).
manufacture_protocol: High-density DNA array prepared with Maskless Array Synthesizer (MAS) technology. See manufacturer's website at http://www.nimblegen.com/.