title: NIA Microarray Mouse Immune Gene Chip (MIC-1)
status: Public on Jun 26 2007
contact.city: Baltimore
contact.first: Supriyo
contact.last: De
contact.state: Maryland
contact.address: 251 Bayview Blvd
contact.department: Laboratory of Genetics and Genomics
contact.country: USA
contact.institute: NIA-IRP, NIH
organism: Mus musculus
description: MIC-1 consists of 4608 cDNA clones that were selected from the initial screening of over 20,000 unique cDNA clones (mouse embryo 15K cDNA clone set from NIA/NIH: http://lgsun.grc.nia.nih.gov/cDNA/15k.html and mouse brain 11K cDNA clones from Brain Molecular Anatomy Project (BMAP), University of Iowa, http://mgc.nci.nih.gov) for expression in mouse spleen and thymus (~4,500) plus additional 90 known genes for cytokines and cytokine receptors that were not in the initial cDNA pool.  Approximately, 64% of the cDNA clones are known genes, the remaining are unnamed genes, and ESTs.  Functionally, the known genes were involved in regulation of cell cycle, growth, and division (10.2%), immune response, chemotaxis, and adhesion (7.6%), signal transduction and transcription regulation (17.6%), apoptosis and cell death (3.1%), protein synthesis, modification, and degradation (12.8%), metabolism (9.7%), DNA packaging and chromatin organization (1.7%).
manufacture_protocol: cDNA were amplified by PCR and printed onto nylon membrane (SuperCharge Nylon, S&S) using MicroGrid II printer (BioRobotics, Apogent Discoveries Inc, Hudson, NH).  The filter is 7.5 x 11 cm2 in size and consisting of 9600 spots at the diameter of 400 micron.  The filter is divided into 384 mini-domain.  Each domain contains 12 cDNA clones that were printed twice and one blank spot as a control for the local background.