title: 4CMenB antigenic components protein microarray
status: Public on Aug 28 2020
contact.city: Siena
contact.first: Erika
contact.last: Bartolini
contact.state: Italy
contact.address: Via Fiorentina 1
contact.department: AIMB
contact.country: Italy
contact.institute: GSK Vaccines
organism: Neisseria meningitidis
manufacture_protocol: Purified recombinant protein were diluted at final concentration of 0.5 mg/ul in 40% glycerol and transferred into 384-well microtiterplates. One droplet of 0.2 nL protein solution was deposited per spot on nitrocellulose-coated slides (FAST slides, Schleicher and Schuell) using the non-contact printer Marathon Spotter (Arrayjet, Edinburgh, UK). Printing was performed in a cabinet with controlled humidity and temperature (55-60% and 12 °C respectively), resulting in spots of 90-110 μm in diameter range. Each protein was printed in 8 replicates on each slide. Printed protein microarrays were kept at room temperature for 5 hours, dried under a stream of nitrogen and store a 4°C.