title: Human V3 AROS
status: Public on Jan 31 2021
contact.city: Jerusalem
contact.first: Inbar
contact.last: Plaschkes
contact.address: The Hebrew University of Jerusalem - Ein Kerem
contact.department: The Faculty of Medicine - Ein Kerem
contact.country: Israel
contact.institute: HUJI
organism: Homo sapiens
manufacture_protocol: The Spotted long oligonucleotide array produced using the Operon Human Genome Oligo Set Version 2.1 (21,329 70mer oligonucleotides, based on build 147 of the Human UniGene database and the Human RefSeq Database, plus 24 controls) and the Operon Human Genome Oligo Set Version 2.1 Upgrade (5,462 70mer oligonucleotides, based on build 13.31 of the Human Ensembl Database).
  Spotting was performed in duplicates (replicate arrays) with 4x6 SMP3 pins (Telechem) at 18-20 °C and 40-50% RH on epoxysilane coated slides (Quantifoil) using 40 µM oligos in FBNC spotting buffer (G. Wrobel et al., Nucleic Acids Res 31 e67, 2003).
  Spot centers were 129 µm apart.
  DNA adhesion was accomplished by 1 h incubation at 60 °C followed by 240 mJ/cm2 UV irradiation at 254 nm.