title: LCS miRHuman 16
status: Public on Oct 04 2016
contact.city: Hangzhou
contact.first: Wengong
contact.last: Si
contact.email: swgjiangda@163.com
contact.state: Zhejiang Province
contact.address: 866 Yuhangtang Road
contact.country: China
contact.institute: Zhejiang university
organism: Homo sapiens
manufacture_protocol: Arrays are made based μParaflo microfluidic chip using a micro-circulation pump (AtacticTechnologies). On the microfluidic chip, each detection probe consisted of a chemically modified nucleotide coding segment complementary to target microRNA (from miRBase, http://microrna.sanger.ac.uk/sequences/) or other RNA (control or customer defined sequences) and a spacer segment of polyethylene glycol to extend the coding segment away from the substrate. The detection probes were made by in situ synthesis using PGR (photogenerated reagent) chemistry.