title: Human 58K oligonucleotide chip
status: Public on Sep 30 2012
contact.city: Odense C
contact.first: Nete
contact.middle: Vinstrup
contact.last: Michelsen
contact.email: nete.michelsen@ouh.regionsyddanmark.dk
contact.address: Sdr Boulevard 29
contact.department: Dept of Clinical Genetics
contact.country: Denmark
contact.institute: Odense University Hospital
organism: Homo sapiens
manufacture_protocol: A human oligonucleotide library composed of 29,134 oligonucleotide targets (60mers) recognising 28,830 genes was purchased from Sigma-Genosys (The Woodlands, TX) and designed by Compugen (Compugen Ltd., Jamesburg, NJ) with a 5´ amino linker modification. The oligonucleotides have been designed to include known genes, expressed sequence tags, the majority of known alternative splice variants (http://www.labonweb.com/oligo), and spike targets.  According to the CodeLinkTM protocol, oligonucleotide targets were solubilized in 150 mM sodium phosphate buffer (pH 8.5) and divided into a single aliquot and applied to 76 384-well source plates using an Eppendorf epMotion 5070 robot (Radiometer, DK) at a final concentration of 20pmol/µl. Oligonucleotide targets were arrayed in duplicate by a spotting robot (Virtek ChipWriter Pro (ESI)) onto 75 slides in a controlled environment with a humidity of 38 % inside the spotting compartment. 12 SMP 2.5 stealth pins (TeleChem International Inc., Sunnyvale, USA) were used for printing. After printing, slides were incubated overnight and blocked as recommended by the manufacturer. Dried microarray slides were stored in a desiccated environment at room temperature.