title: SNI_human_MPSS
status: Public on May 06 2010
contact.city: Seattle
contact.first: Biaoyang
contact.last: Lin
contact.email: bylin@u.washington.edu
contact.state: WA
contact.address: 550 17th Ave, James Tower suite 550
contact.department: Swedish Neuroscience Institute
contact.country: USA
contact.institute: Swedish Medical Center
contact.phone: 206 320 3570
organism: Homo sapiens
manufacture_protocol: The mRNA was processed according to the MPSS protocol as outlined in the publications : Brenner, S., et al. (2000) Proc Natl Acad Sci U S A 97(4):1665-1670 and Brenner, S. et al., Nat. Biotechnol 18(6): 630-634. Briefly, the mRNA was reverse transcribed and the cDNA was digested with DpnII. The cDNA fragment starting at the 3' most DpnII site and ending with the polyA was cloned into a Megaclone vector library. The resulting library was amplified and loaded onto microbeads. About 1.6 million microbeads were loaded into each flow cell and the signature sequences were determined by a series of enzymatic reactions as outlined in the above publications. The abundance for each signature was converted to transcripts per million (tpm) for the purpose of comparisons between samples.