title: R & D Human Cytokine Array
status: Public on Apr 15 2010
contact.city: Central
contact.first: R A P 
contact.middle: M
contact.last: Perera
contact.email: h0696905@hkusua.hku.hk
contact.address: 34, Hospital road
contact.department: Faculty of Dentistry
contact.country: Hong Kong
contact.institute: The University of Hong kong
organism: Homo sapiens
manufacture_protocol: Proteome Profiler 96 Microplate-based Antibody Arrays consist of a 96-well microplate pre-spotted with multiple capture antibodies in each well. Experimental samples are added and target proteins present in the samples bind to the immobilized antibodies. All wells also contain Reference Spots, which provide a strong positive signal to allow easy visualization of well location. A cocktail of biotinylated detection antibodies and Streptavidin-HRP or an HRP-conjugated pan anti-phospho-tyrosine antibody is subsequently used to detect the bound proteins. Chemiluminescent substrate reagents produce a signal that is proportional to the amount of analyte bound. A compatible digital imaging system capable of chemiluminescent detection is used to determine the intensity of light emitted from individual spots.