title: University of Utah Schmidtea mediterranea cDNA array generation I
status: Public on Aug 26 2008
contact.city: Salt Lake City
contact.first: Alejandro
contact.last: Sánchez Alvarado
contact.email: sanchez@neuro.utah.edu
contact.state: UT
contact.address: 20N 1900E
contact.department: Neurobiology and Anatomy
contact.country: USA
contact.web_link: http://planaria.neuro.utah.edu
contact.institute: University of Utah School of Medicine
organism: Schmidtea mediterranea
manufacture_protocol: 3,435 cDNAs from two asexual cDNA libraries, a neoblast-enriched cell population (NB) and animal heads (H) (Sánchez Alvarado et al., Development. 129:5659-5665), were printed in triplicate on Corning UltraGAPS glass microscope slides using an Amersham BioSciences Lucidea Array Spotter.  The PCR products were prepared for spotting by diluting the samples in a solution of 50% DMSO to an average final concentration of 100 ng/ul.  Following the print session, the DNA targets were cross-linked to the slide surface at 250mJ using a Stratagene Stratalinker.