title: The Use of a Soybean cDNA Microarray to Assess for Differential Gene Expression in Vicia Species: A Mixed Model Approach
status: Public on Oct 30 2005
contact.city: Fort Collins
contact.first: Kathleen
contact.last: Yeater
contact.email: kathleen.yeater@ars.usda.gov
contact.state: CO
contact.address: 2150 Centre Avenue
contact.country: USA
contact.institute: USDA-ARS
organism: Glycine max
  Vicia villosa
  Vicia pannonica
manufacture_protocol: Printer was a Cartesian Pyxis 5500
  
  PCR primers:
  
  F NIA-M13F CCAGTCACGACGTTGTAAAACGAC
  R NIA-M13R GTGTGGAATTGTGAGCGGATAACAA
  
  PCR condition:
  50 ul reactions
  95oC 10 min
  35 cycles of 95oC 40 sec, 65oC 40 sec, 72oC 3 min
  72oC 5 min
  4oC 1 min
  
  All products were run out on 1.5% agarose gels. Failed reactions were rerun and replaced. The entire reaction was cleaned on Sephadex columns in Whatman filter plates, transferred to a 384 well plate,
  dried down in a speedvac and resuspended in printing buffer.
  
  There was one grid, double spotted with 185 x 185 micron spacing. 8 columns and 39 rows.