title: Laval/Sirard_human-bovine_granulosa_2.5K_V1.0
status: Public on Jul 10 2010
contact.city: Québec City
contact.first: Marc-André
contact.last: Sirard
contact.email: Marc-Andre.Sirard@fsaa.ulaval.ca
contact.state: Quebec
contact.address: Offfice 2732, 2440 Hochelaga Blvd.
contact.department: Sciences Animales
contact.country: Canada
contact.institute: Université Laval
organism: Homo sapiens
  Bos taurus
manufacture_protocol: cDNA was obtained by SSH experiments (Clontech). The substractions were done on granulosa cells collected at the same time as competant versus incompetant oocytes from bovine and human tissues.competant Each clone was amplified by PCR, purified, dry-down and resuspended in H20/DMSO(50:50). The clones were spotted using a chipwritter (Virtek) on ultra-glass slides (Corning). The slides were UV treated occording to the manufacturer's protocole.