title: NanoString nCounter Gene Expression
status: Public on Oct 05 2021
contact.city: Chapel Hill
contact.first: Xiaohua
contact.last: Gao
contact.email: xiaohua_gao@med.unc.edu
contact.state: NC
contact.address: 450 West Dr
contact.department: Lineberger Comprehensive Cancer Center
contact.country: USA
contact.institute: UNC
contact.phone: 9197375638
organism: Homo sapiens
manufacture_protocol: Briefly, RNA was isolated from a 1-mm FFPE core or two 10-μm unstained FFPE slides using the Qiagen RNeasy FFPE Kit and protocol (catalog no. 73504). RNA was quantitated using a Thermo Fisher Scientific NanoDrop 2000 Spectrophotometer (catalog no. ND-2000) and Agilent 4200 TapeStation. After excluding samples with low concentration or low percentages of RNA molecules >300 nucleotides long, the remaining 90% of samples were processed by the UNC Translational Genomics Laboratory using the NanoString nCounter platform.