title: Songbird Neurogenomics Initiative 20K Array
status: Public on Nov 11 2009
contact.city: Urbana
contact.first: Kirstin
contact.last: Replogle
contact.email: replogle@igb.uiuc.edu
contact.state: IL
contact.address: 1206 W Gregory Drive
contact.country: USA
contact.institute: University of Illinois
organism: Taeniopygia guttata
manufacture_protocol: cDNAs are arrayed in Genetix 384 well plates and stored frozen. Plates are thawed and spun down.
  Plates are placed in storage hotel of an Omnigrid 100 (Genomic Solutions)
  Slides are printed in batches of 100 using 1.5M Betaine + 3 x SSC
  Following printing slides are baked at 80C for 2 hours
  Slides are washed in 0.2% SDS followed by three water washes and an ethanol wash
  Slides are dried by spinning in a centriguge at 1000 RPM for 3 minutes
  Slides are stored in orange Corning boxes 25 per box
  Storage boxes are purged with argon and sealed under vacuum