title: Applied Biosystems TaqMan TLDA 64-Breast cancer associated-gene panel
status: Public on Sep 30 2014
contact.city: San Francisco
contact.first: Mark
contact.last: Magbanua
contact.state: CA
contact.address: 1450 3rd Street, PO Box 589001
contact.department: HemOnc
contact.country: USA
contact.institute: UCSF/Helen Diller Familiy Comprehensive Cancer Center
organism: Homo sapiens
description: We selected 64 genes from previously published data focused on QPCR analyses of CTCs. Included in the list are clinically relevant genes, stem cell and epithelial-mesenchymal transition (EMT) markers, and highly expressed genes in breast and prostate cancers. We also included EPCAM and PTPRC (encodes CD45) to measure the expression of these markers specific for epithelial cells and leukocytes, respectively. Also, included are 6 candidate reference genes for data normalization.
  We designed a custom Taqman® Low Density Array (TLDA, Applied Biosystems) microfluidic card (384-well format) containing two sets of 64 Taqman® gene expression assays printed in triplicate.
manufacture_protocol: see manufacturer's website