title: Plants 4K miRNA LC_MRA-1038_miRNAPlants_11.0 + Bn_PsRNAs
status: Public on Dec 15 2009
contact.city: Madrid
contact.first: Anja
contact.last: Buhtz
contact.email: anja.buhtz@upm.es
contact.address: Campus de Montegancedo, M40,km38
contact.country: Spain
contact.institute: Centro de Biotecnologia y Genomica de Plantas
organism: Physcomitrium patens
  Pinus taeda
  Populus trichocarpa
  Arabidopsis thaliana
  Brassica napus
  Brassica rapa
  Brassica oleracea
  Glycine max
  Medicago truncatula
  Oryza sativa
  Saccharum officinarum
  Sorghum bicolor
  Triticum aestivum
  Zea mays
  Vitis vinifera
  Selaginella moellendorffii
description: Plant miRNA microarray with 100% coverage of mature miRNAs listed in miRBase version 11.0 and additional custom small RNA sequences from Brassica napus phloem sap (Bn_PsRNAs)
manufacture_protocol: Arrays are made based µParaflo microfluidic technology (Atactic Technologies). On the µParaflo microfluidic chip, each detection probe consists of a chemically modified nucleotide coding segment complementary to target microRNA (from miRBase, http://microrna.sanger.ac.uk/sequences/) or other RNA (control or customer defined sequences) and a spacer segment of polyethylene glycol to extend the coding segment away from the substrate. The detection probes are made by in situ synthesis using PGR (photogenerated reagent) chemistry.