title: Pleiss Lab_S.cerevisiae_Q-PCR Screen Library
status: Public on May 15 2012
contact.city: Ithaca
contact.first: Jeffrey
contact.middle: A
contact.last: Pleiss
contact.email: jpleiss@cornell.edu
contact.state: NY
contact.address: 451 Biotech
contact.department: Molecular Biology and Genetics
contact.country: USA
contact.institute: Cornell University
organism: Saccharomyces cerevisiae
manufacture_protocol: The S. cerevisiae strains from Open Biosystems were arrayed onto 14 384-well growth plates. Another set of temperature senzitive strains from Phil Hieter's lab were arrayed on the 15th 384-well plate together with conditional alleles derived from the Guthrie lab. RNA was extracted from these strains in a high-throughput manner, followed by cDNA synthesis and Q-PCR. The set comprises about 5500 mutants.