title: The M.D. Anderson Cancer Center (MDACC) miRNA Expression Bioarrays
status: Public on May 25 2013
contact.city: Houston
contact.first: Yiqun
contact.last: Zhang
contact.email: yiqunz@bcm.edu
contact.address: One Baylor plaza
contact.country: USA
contact.institute: BCM
organism: Homo sapiens
manufacture_protocol: The M.D. Anderson Cancer Center (MDACC) miRNA Expression Bioarrays are transcriptional profiling products designed to monitor the miRNA and other small non-coding RNA levels of multiple genes. The miRNA Expression Bioarrays utilize nucleic acid hybridization of a 52 biotin-labeled complementary cDNA target with DNA oligonucleotide probes attached to a gel matrix. The biotin-labeled cDNA targets are prepared by a simple reverse transcription into first strand cDNA. Total RNA is primed for reverse transcription by a random Octomer conjugated with two biotins and a 52 poly (A) tail. This procedure results in an equal copy number of biotin cDNA target to the templates of miRNA.