title: PEPperCHIP Autoimmunity Microarray
status: Public on Feb 28 2015
contact.city: Birmingham
contact.first: Jennie
contact.last: Hamilton
contact.state: AL
contact.address: 1825 University Blvd.
contact.department: Department of Medicine, Division of Clinical Immunology and Rheumatology
contact.country: USA
contact.institute: University of Alabama Birmingham
organism: Klebsiella pneumoniae
  Proteus mirabilis
  Mycobacterium tuberculosis
  Homo sapiens
  Human alphaherpesvirus 2
  human gammaherpesvirus 4
  Caprine arthritis encephalitis virus
  Human endogenous retrovirus K
  Torque teno virus
description: Laser printer spotted peptides
  All source information for each peptide is available on the Immune Epitope Database (http://www.iedb.org/).
manufacture_protocol: Amino acid microparticles are laser printed onto a glass slide with a custom 24-color laser printer.  One layer of an amino acid particle pattern is printed on a coated glass slide. A heating step melts the particles.  Embedded amino acids can couple to the chip surface.  In accordance with solid phase Fmoc chemistry, a cycle of synthesis is completed after removal of excessive material by washing and cleavage of the N-terminal Fmoc protection group. The cycle is repeated until the full peptide microarray is synthesized.