title: Silkworm Microarray 22k v1
status: Public on Aug 28 2009
contact.city: BeiBei
contact.first: Qingyou
contact.last: Xia
contact.email: xiaqy@swu.edu.cn
contact.state: Chongqing
contact.address: No.2 Tiansheng Road
contact.country: China
contact.institute: Southwest University
contact.phone: 086-23-68250099
organism: Bombyx mori
manufacture_protocol: All sequences that were regarded as probe designing models were derived from the draft silkworm genome sequence in SilkDB (http://silkworm.genomics.org.cn/; xia et al. 2004) and other resources available in GenBank. These data set contained 21,302 predicted genes (including 13,093 complete and 8,209 partial) from WGS, 212 full-length cDNA sequences from GenBank, and 16 425 Unigens based on ~150000 ESTs from different silkworm tissues. We also integrated 554 GenBank genes of other Lepidoptera and 521 silkworm genes homologous to other lepidopteran insect genes.
  A 70-mer oligonucleotide modified at 5' ends with a 6-carbon linker and a primary amine corresponding to each sequence was designed by CapitalBio Corporation (Beijing, China) and synthesized by MWG Biotech (Ebersberg, Germany). Then Oligonucleotides were dissolved in EasyArrayTM spotting solution (CapitalBio Corp.) at 40μM concentration. Probe was printed on amine silaned slides using a SmartArrayTM microarrayer (CapitalBio Corp.). On the slide, there are 48 blocks and every block has 23 columns and 22 rows. Probes for four house keeping genes and eight yeast intergenic sequences were printed on every block used as internal and external controls respectively.