title: YCW_Human_3.6KBAC_ARRAY
status: Public on Jul 25 2012
contact.city: Tainan
contact.first: Yi-Ching
contact.last: Wang
contact.email: ycw5798@mail.ncku.edu.tw
contact.address: No.1, University Road, Tainan 70101, Taiwan, R. O. C.
contact.department: Department of Pharmacology, College of Medicine
contact.country: Taiwan
contact.institute: National Cheng Kung University
contact.phone: +886-6-2353535
organism: Homo sapiens
description: build 35 - hg17 of UCSC Genome Browser
manufacture_protocol: The genomic microarray used containing 3604 BAC clones representing the human genome at 18 non-gap chromosome regions. Clone mapping was analyzed using the BAC end pairs database of UCSC Genome Bioinformatics (http://genome.ucsc.edu/). The BAC library used was RP-11, which was provided from Genome Research Center, University of Yang-Ming. The extracted BAC DNA was amplified to 10 μg by Phi29 DNA polymerase reaction (TempliPhi DNA Sequencing Template Amplification Kit, Amersham, Princeton, NJ), and digested by DNaseI (New England Biolabs, Ipswich, MA), and purified by MultiScreen-PCR Plates (Millipore, Danvers, MA). After digestion, the DNA fragments were resolved by gel electrophoresis and the fragment size were optimized to within 0.5–3 kb. Finally, the purified BAC DNA fragments were dotted triplicate in 72 blocks on the glass slides (Corning, NY, USA) with spotting solution (50% DMSO, 50% D2H2O).