title: FHCRC Human 18K Array
status: Public on May 17 2005
contact.city: Seattle
contact.first: Pavel
contact.last: Sova
contact.email: ps44@u.washington.edu
contact.state: WA
contact.address: 325 9th Avenue
contact.department: Department of Pathology
contact.country: USA
contact.institute: University of Washington
contact.phone: 206/341-4705
organism: Homo sapiens
description: Microarray construction and hybridization protocols were modified from DeRisi, J.L., V.R Iyer, and P.O. Brown. Science 278:680-686, 1997. The microarrays were constructed using a set of more than 17000 sequence-verified clones from Research Genetics (UniGene Build 136). The cDNA inserts were amplified from replica plate cultures by PCR, purified and mechanically spotted onto polylysine-coated microscope slides using an OmniGrid robotic spotter (GeneMachines, San Carlo, CA).