title: Qiagen Mouse Inflammatory Response & Autoimmunity miRNA PCR Array (MIMM-105Z)
status: Public on Mar 13 2018
contact.city: Bloomington
contact.first: Patricia
contact.last: Silveyra
contact.email: psilveyr@iu.edu, ptrcslvr@gmail.com
contact.state: Indiana
contact.address: 2819 E 10th St
contact.department: Environmental and Occupational Health
contact.country: USA
contact.institute: Indiana University Bloomington
organism: Mus musculus
description: PCR assay
  The Mouse Inflammatory Response & Autoimmunity miScript miRNA PCR Array profiles the expression of 84 miRNAs predicted to regulate the expression of proinflammatory or antiinflammatory genes. This array provides researchers with a convenient way to quickly analyze the miRNAs most likely to be relevant to inflammatory and autoimmune disorders. These miRNAs have been carefully selected based on those predicted by bioinformatic algorithms and databases to regulate genes known to be relevant to inflammation. The profiling results from this array can serve as a useful molecular marker for the study of normal and pathological inflammation. The results may also provide a deeper understanding of the molecular mechanisms regulating inflammatory gene expression by miRNA. A set of controls present on this array enables data analysis using the ΔΔCT method of relative quantification, assessment of reverse transcription performance, and assessment of PCR performance. Using SYBR Green-based real-time PCR, the expression of a focused panel of miRNAs predicted to regulate inflammatory and autoimmune response genes can be easily and reliably analyzed with this miScript miRNA PCR Array.
manufacture_protocol: See manufacturer's website