title: Phytoplasma asteris OY-M DNA array
status: Public on Jan 16 2012
contact.city: Bunkyo
contact.first: Kenro
contact.last: Oshima
contact.email: kenro@ims.u-tokyo.ac.jp
contact.state: Tokyo
contact.address: 1-1-1, Yayoi
contact.country: Japan
contact.institute: The University of Tokyo
organism: Candidatus Phytoplasma asteris
description: OY-M strain
manufacture_protocol: 1.About 300 bp of DNA are amplified for each gene from OY-M phytoplasma genome, purified, and resuspended to 0.1 ug/ul dH2O. The 5'-end of primer used with amplification is labeled with amino residue.
  2. Add equal volume of the spotting buffer to the cDNA solution.
  3. Spot to TaKaRa-Hubble Slide Glass.
  4. After the print run is completed, process the fixation protocol of the glass.