title: UDMP Homo sapiens MitoArray 6k v1
status: Public on Jul 01 2007
contact.city: Prague
contact.first: Viktor
contact.last: Stranecky
contact.address: Ke Karlovu 2
contact.department: 1st Faculty of Medicine
contact.country: Czech Republic
contact.institute: Charles University
organism: Homo sapiens
description: Microarray: 5760 spots
  24 blocks: 4x6
  each block: 15x16 spots
manufacture_protocol: For each of selected 1632 genes, a single 5´-aminomodified 40-mer oligonucleotide was designed using Oligopicker software (see the Parallel Biological Systems web page, by the Massachusetts General Hospital, Harvard Medical School, and Boston University).  Blast searches were performed with each candidate probe to exclude possibility of cross hybridization with homologous genes prior the synthesis oligonucleotide probes. Synthesized oligonucleotides, Generi Biotech (Czech Republic) and Illumina (San Diego, Ca), were resuspended at 20µM concentration in 3*SSC, printed in triplicates on aminosilane modified slides and immobilised by standard technique using combination of backing and UV cross-link. Qualities of arrays from individual printing series were assessed using fluorescently labelled panomers (Invitrogen, Carlsbad, Ca).