title: ENS SGDB Mus musculus NeuroDev v2.2
status: Public on Jun 30 2008
contact.city: Paris
contact.first: Stéphane
contact.last: LE CROM
contact.address: 46 rue d'Ulm
contact.department: Biology Institute - IBENS
contact.country: France
contact.institute: École normale supérieure
organism: Mus musculus
description: NeuroDev mouse slides were designed using PCR products (produced by the transcriptome platform) that represent around 2000 cDNA by slide linked to the developement of the mouse nervous system. A large part of these clone are a subset of the NIA 15k collection where the clone name have been selected according to their implication in the central nervous system developpement.
  Each clone has been spotted in duplicate
  PCR products were amplified by the National Sequencing Center.
manufacture_protocol: Slide spotted using a MicroGrid II (Genomic Solution)
  Spotting conditions: Temperature of 20¡C more or less 5¡C. Hygroscopy of 50% more or less 5%.
  Before each spotting:
  1.	Printing tips (Geneworx 10k) are tested on glass slides.
  2.	The spotting robot is put in operation and water tanks filled with distilled water.
  3.	Corning glass slides are deposited on plates.
  4.	The spotting program specific for each slide bacth is launched.