title: Daphnia magna DGC cDNA array ver.1
status: Public on Dec 22 2006
contact.city: Boston
contact.first: Helen
contact.middle: C
contact.last: Poynton
contact.email: helen.poynton@umb.edu
contact.state: MA
contact.address: 100 Morrissey Blvd.
contact.department: School for the Environment
contact.country: USA
contact.institute: UMass Boston
contact.phone: 617-287-7323
organism: Daphnia magna
manufacture_protocol: The 5000 randomly selected cDNA clones from the Daphnia Genome Consortium (DGC) library (generous gift from D. Bauer at University of New Hampshire, NH and J. Colbourne at Indiana University, IN) were PCR amplified from the pDNR-LIB vector using the following primers: forward: AGTCGACGGTACCGGACATA and reverse: GCCAAACGAATGGTCTAGAAA.  PCR products were purified by ethanol precipitation and resuspended in distilled water. cDNA clones were printed onto lysine-coated glass slides (Cheung et al. 1999).
  Cheung VG, Morley M, Aguilar F, Massimi A, Kucherlapati R, Childs G.  1999.  Making and reading microarrays. Nat Genet 21:15-9.