title: USP Blastocladiella emersonii 4.6 K Blasto3773 v3
status: Public on Jan 05 2009
contact.city: Goiania
contact.first: Silvia Maria
contact.last: Salem-Izacc
contact.email: silviaizacc@icb.ufg.br
contact.state: Goias
contact.address: ICB, Campus 2
contact.department: Bioquimica e Biologia Molecular
contact.country: Brazil
contact.institute: Universidade Federal de Goiás
organism: Blastocladiella emersonii
manufacture_protocol: The array consists of cDNA inserts from 3,563 distinct B. emersonii expressed genes obtained through Georg and Gomes (2007) and Ribichich et al. (2005) works. These inserts were PCR amplified with the T7 forward and SP6 reverse primers. The amplified products were purified in Millipore Multiscreen Filtration Plates (MAFB NOB), eluted with Tris-HCl 10 mM, pH 8.0 to a final concentration of 100 to 200 ng/μl and spotted onto Star 7 slides (Amersham Biosciences) using a Generation III Microarray SpotterTM (Amersham Biosciences).