title: MPIMarburg_Piriformospora_indica_105k_v1.0
status: Public on Oct 01 2011
contact.city: Regensburg
contact.first: Urs
contact.last: Lahrmann
contact.address: Am Biopark 9
contact.department: Project Group Personalized Tumor Therapy
contact.country: Germany
contact.institute: Fraunhofer ITEM
organism: Serendipita indica
manufacture_protocol: Both catalog and custom microarrays are manufactured using a proprietary non-contact industrial inkjet printing process, in which oligo monomers are deposited uniformly onto specially-prepared glass slides. This in situ synthesis process prints 60-mer length oligonucleotide probes, base-by-base, from digital sequence files. The precise inkjet process enables the delivery of extremely small, accurate volumes (picoliters) of the chemicals to be spotted. Standard phosphoramidite chemistry used in the reactions allows for very high coupling efficiencies to be maintained at each step in the synthesis of the full-length oligonucleotide. Precise quantities are reproducibly deposited “on the fly.” This engineering feat is achieved without stopping, to make contact with the slide surface and without introducing surface-contact feature anomalies, resulting in consistent spot uniformity and traceability. In addition, multiple microarrays can be printed simultaneously in a single manufacturing run.