title: VUMC MACF human 19K oligo vSAE4
status: Public on Dec 31 2005
contact.city: Amsterdam
contact.first: Daoud
contact.last: Sie
contact.email: d.sie@vumc.nl
contact.address: De Boelelaan 1117
contact.department: Pathology
contact.country: Netherlands
contact.institute: Vrije Universiteit Medical Center
contact.phone: +31 20 4442428
organism: Homo sapiens
description: - oligonucleotide sequences are available via www.ensemble.org after agreeing to Compugen's conditions
  - oligonucleotides were mapped by Ensembl to the NCBI35 version of the human genome
manufacture_protocol: The Human Release 1.0 oligonucleotide library, containing 18861 60-mer oligonucleotides representing 17260 unique genes as designed by Compugen (San Jose, CA, USA) was obtained from Sigma-Genosys (Zwijndrecht, The Netherlands). The oligonucleotides were dissolved at 10 µM concentration in 150 mM sodium phosphate buffer pH 8.5 and single spotted onto CodeLink slides (Amersham Biosciences) using a SpotArray Enterprise non-contact microarray printer (Perkin Elmer). After printing slides were processed and blocked prior to use according to the manufacturer’s protocol.