title: MPIMG Homo sapiens 43K aCGH2_MPIMG_BERLIN
status: Public on Oct 19 2006
contact.city: Berlin
contact.first: Reinhard
contact.last: Ullmann
contact.email: ullmann@molgen.mpg.de
contact.address: Ihnestr.73
contact.department: Human Molecular Genetics
contact.country: Germany
contact.institute: MPIMG
contact.phone: 00493084131251
organism: Homo sapiens
manufacture_protocol: This BAC array consists of  the tiling path for chromosomes 4, 9, 10, 11, 16, 17, 21, 22  and X, derived from the human 32k BAC Re-Array Set (BAC PAC resources; DNA kindly provided by Pieter de Jong), the 1Mb Sanger set (clones kindly provided by Nigel Carter, Wellcome Trust Sanger Centre) and a set of subtelomeric clones (assembled by members of the COSTB19 Action: Molecular cytogenetics of solid tumours). Protocols for the isolation of DNA (1Mb Sanger set and subtelomeric set) and the amplification of insert DNA by linker adapter PCR are described in detail at Molecular Cytogenetics web site of MPG. Amplicons were ethanol precipitated, dissolved in 3xSSC/1,5M betaine and spotted on epoxy-coated slides (NUNC, Wiesbaden, FRG) by means of a modified Qarray (originally from Genetix, new Milton, U.K.) and Pointech (Gibbon, MN) Tungston PTL 2500 slit pins.
  DNA is spotted in triplicates.