title: OHSU_macaque_RRV_252_v1
status: Public on Aug 29 2008
contact.city: Beaverton
contact.first: Scott
contact.middle: William
contact.last: Wong
contact.email: wongs@ohsu.edu
contact.state: OR
contact.address: 505 NW 185th Avenue
contact.department: Vaccine and Gene Therapy Institute
contact.country: USA
contact.institute: Oregon Health & Science University
contact.phone: 5036905285
organism: Macaca mulatta rhadinovirus 17577
manufacture_protocol: Arrays were printed onto UltraGAPS slides (Corning) using a PixSys 5500XL microarray printer (Cartesian) and CMP3 pins (Telechem).  Spots were made from 1-2 nl of solution with a center-to-center distance of 183 microns.  Arrays were printed in duplicate on each slide.  After printing arrays were baked for 3.5 hours at 80 C, then stored in a dark dessicator until use.  Prior to use arrays were blocked with 2% BSA in 5x SSC / 0.1% SDS.