title: ORNL-ESD Geobacter metallireducens 70mer oligo-array
status: Public on Jul 03 2007
contact.city: Knoxville
contact.first: Qiang
contact.last: He
contact.email: qianghe@utk.edu
contact.state: TN
contact.address: 57A Perkins Hall
contact.department: Civil and Environmental Engineering
contact.country: USA
contact.institute: The University of Tennessee
contact.phone: 865-974-6067
organism: Geobacter metallireducens GS-15
description: In total, 4129 probes were designed based on an early version of the gene model (2003) and printed on the microarray, among which 3727 probes have 1 perfect match with one single ORF; 98 probes have more than 1 perfect matches with more than 1 ORFs; 261 probes have at least 1 perfect match with the genome, but not with an ORF; and 43 probes have no perfect match with the genome.
manufacture_protocol: All 70mer oligonucleotides were commercially synthesized without modification by MWG Biotech Inc. (High Point, NC). The concentration of oligonucleotides was adjusted to 100 pmol/µl. Oligonucleotide probes were prepared in 50% vol/vol DMSO (Sigma-Aldrich, St. Louis, MO) and spotted onto UltraGAPS glass slides (Corning Life Sciences, Corning, NY) using a BioRobotics Microgrid II microarrayer (Genomic Solutions, Ann Arbor, MI). Each oligonucleotide probe had two replicates on a single slide. After printing, the oligonucleotide probes were fixed onto the slides by UV cross-linking (600 mJ of energy) according to the protocol of the manufacturer of the UltraGAPS glass slides (Corning Life Science, Corning. NY).