title: CUFSL Listeria interalins 384 v1
status: Public on Dec 01 2006
contact.city: Ithaca
contact.first: Patrick
contact.last: Mc Gann
contact.email: pm223@cornell.edu
contact.state: NY
contact.address: 412 Stocking Hall
contact.department: Food Science
contact.country: USA
contact.institute: Cornell University
contact.phone: (607) 227 0627
organism: Listeria monocytogenes
manufacture_protocol: Oligonucleotides were resuspended in 1× SSC, 0.0025% Sarcosyl to a final molarity of 30uM. All oligonucleotides and controls were spotted in duplicate on Amino-silane coated Corning UltraGAPS slides (Corning, NY USA) using a custom built XYZ microarrayer. Oligonucleotides were UV-crosslinked at 300mJ for 1 minute. Printing quality was checked using Cy3-labeled random sequence nonamers