title: NIAID Mmca -- Mouse 38K
status: Public on May 02 2008
contact.city: Bethesda
contact.first: Timothy
contact.middle: G
contact.last: Myers
contact.email: tgm@nih.gov
contact.state: MD
contact.address: 50 South Drive, Room 5509
contact.department: Research Technologies Branch
contact.country: USA
contact.institute: National Institute of Allergy and Infectious Diseases
organism: Mus musculus
manufacture_protocol: Microarrays are printed with an Omnigrid robotic arrayer by Genomic Solutions ( http://www.genomicsolutions.com ) that is fitted with a printhead and 48 SMP2 print tips from Telechem ( http://www.arrayit.com ).
  The mouse genome microarray (Mmca) probe set is based on 70mer oligonucleotides from Operon's (AROS) Mouse Genome Oligo Set Version 4.0 ( http://omad.operon.com/download/index.php ).
  Oligonucleotide probes are distributed into 384-well polypropylene plates (Genetix, 384 micro array) and dissolved in print buffer (150mM sodium phosphate, pH 8.5, 4% sodium sulfate, 0.0001% N-lauryl sarcosyl)
  to a normalized 25 micromolar concentration. Spot densities are 155 microns (center-to-center).
  The microarrays are printed on Epoxy Slides from Corning (Catalog # 40042) and treated by a method that is an adaptation of the manufacturer's instructions to allow higher throughput processing.