title: University of Victoria/Caren Helbing Laboratory Frog MAGEX DNA array version 2008
status: Public on Feb 21 2013
contact.city: Victoria
contact.first: Nik
contact.last: Veldhoen
contact.email: veldhoen@uvic.ca
contact.state: BC
contact.address: PO Box 3055 STN CSC
contact.department: Biochemistry & Microbiology
contact.country: Canada
contact.institute: University of Victoria
contact.phone: 250-721-7086
organism: Xenopus laevis
  Aquarana catesbeiana
  Lithobates pipiens
manufacture_protocol: MAGEX 2008 cDNA microarrays were printed on Erie C28 aminosilane (ThermoFisher Scientific) coated slides with a QArraymax microarray printer equipped with ArrayIt Stealth Technology SMP2 quill pins (TeleChem International Inc.) in a 48-pin tool that deposited ~ 0.1ng cDNA pieces per spot.  The cDNA fragments were spotted in 16 subgrids, each subgrid is 11x11 containing 119 spots (plus 2 blanks) with a spot pitch of 220 microns.  The spots have a diameter range of 80-100 microns and are spotted in triplicate, twice on each slide (i.e. two identical arrays (or fields) per slide).  Each subgrid also included negative controls (empty spots, 3X SSC buffer-only spots, and Arabidopsis thaliana PCR product) and corner markers (a 68 base GFP (Green Fluorescent Protein) oligo provided by the Microarray Facility of The Prostate Centre at Vancouver General Hospital).