title: ENS SGDB Mus musculus 22k Compugen
status: Public on Jul 04 2008
contact.city: Paris
contact.first: Stéphane
contact.last: LE CROM
contact.address: 46 rue d'Ulm
contact.department: Biology Institute - IBENS
contact.country: France
contact.institute: École normale supérieure
organism: Mus musculus
description: Mouse OligoLibraryTM. The Mouse OligoLibraryTM by Compugen and Sigma-Genosys combines Compugen's superior computational platform and oligo design tools with Sigma's high-quality oligo synthesis.
  Oligo length: 65 bases.
  Modification: 5Õ-C6 amino modifier.
  Number of oligos: 21,997.
  Number of genes: 21,587 (excluding controls).
  Positive controls: four oligos per plate from glyceraldehyde-3-phosphate dehydrogenase (GAPDH).
  Distance from the 3Õ end: 95% of the oligos are within the range of 65Ð1,072 bases from the 3Õ end. Average distance from the 3Õ end is 379 bases.
  Cross-homology: 90% of the oligos contain a predicted cross-homology of less than 81.5%. Average cross-homology is 37.7%.
  Longest full match stretch: in addition to cross-homology percentage, oligos are also selected with a minimal length of sequence identity in the cross-homology alignment.
  GC content: 95% of the oligos contain a GC content within the range of 40%Ð60%. Average GC content is 49.9%.
manufacture_protocol: Slide spotted using a MicroGrid II (Genomic Solution)
  Spotting conditions: Temperature of 20¡C more or less 5¡C. Hygroscopy of 50% more or less 5%.
  Before each spotting:
  1.	Printing tips (Geneworx 10k) are tested on glass slides.
  2.	The spotting robot is put in operation and water tanks filled with distilled water.
  3.	Corning glass slides are deposited on plates.
  4.	The spotting program specific for each slide bacth is launched.