title: TAGC Homo sapiens 9k (HuSG9k) v1
status: Public on Mar 22 2011
contact.city: Lyon
contact.first: Julien
contact.last: Textoris
contact.email: julien.textoris@gmail.com
contact.address: Hôpital Edouard herriot - Pavillon P; 5 place d'Arsonval
contact.department: Medical Diagnostic Discovery Department (MD3)
contact.country: France
contact.institute: bioMérieux
contact.phone: +33 472 119 546
organism: Homo sapiens
description: Nylon based microarray with cDNA probes
manufacture_protocol: Briefly, HuSG9k is a human cDNA nylon chip designed from IMAGE clones. The microarray contained 9216 spots. Among 8682 genes, 100 genes are duplicated with another clone. Four hundred and thirty four probes served as controls. The microarray design was not enriched in specific pathways. Genes with known functions were selected when predicted genes or genes with unknown function were discarded. Probes were selected by bioinformatics analysis to match the 3' end of the major transcripts of each gene (Microarray Quality Control (MiQC), TAGC, INSERM U928; Marseilles, France). PCR products were spotted onto nylon membranes (Hybond-N+, Amersham) with a MicroGrid II arrayer (Affymetrix, Santa Clara, CA, USA). PCR amplification products of selected clones were 1 500 bp length on average. The robot used a print head of 16x4 arrangement of print tips. Each tip printed 144 spots in a 12x12 grid. Further details on the HuSG9k microarray are available on the TAGC website (http://tagc.univ-mrs.fr/).