title: MPIMG Homo sapiens 44K aCGH2_MPIMG_BERLIN
status: Public on Sep 12 2006
contact.city: Berlin
contact.first: Reinhard
contact.last: Ullmann
contact.email: ullmann@molgen.mpg.de
contact.address: Ihnestr.73
contact.department: Human Molecular Genetics
contact.country: Germany
contact.institute: MPIMG
contact.phone: 00493084131251
organism: Homo sapiens
manufacture_protocol: The submegabase resolution whole genome tiling path BAC array consists of the human 32k BAC Re-Array Set* (BACPAC Resources Center; DNA kindly provided by Pieter de Jong), the 1Mb Sanger set (clones kindly provided by Nigel Carter, Wellcome Trust Sanger Centre) and a set of subtelomeric clones (assembled by members of the COSTB19 Action: Molecular cytogenetics of solid tumours). Protocols for the isolation of DNA (1Mb Sanger set and subtelomeric set) and the amplification of insert DNA by linker adapter PCR are described in detail at the Molecular Cytogenetics website of MPG. Amplicons were ethanol precipitated, dissolved in 3xSSC/1,5M betaine and spotted on epoxy-coated slides (NUNC, Wiesbaden, FRG) by means of a modified Qarray (originally from Genetix, new Milton, U.K.) and Pointech (Gibbon, MN) Tungston PTL 2500 slit pins.
  Each BAC is represented by a single spot on the array.
  * due to recurrent abnormal ratios in a series of control hybridisation encompassing 30 tumors, clones from plate HGP DNA RP C 12_2 were excluded from further analysis