title: Candida_albicans_ArrayII_v5
status: Public on Jun 15 2009
contact.city: Boston
contact.first: Anna
contact.last: Selmecki
contact.email: selme003@umn.edu
contact.state: MA
contact.address: 450 Brookline Ave
contact.country: USA
contact.institute: Dana-Farber Cancer Institute
organism: Candida albicans
description: This platform has a different probe layout and different printing date than the other platforms.
manufacture_protocol: Array production and printing were done as described for ArrayI and ArrayII platforms. Primer pairs were used to amplify Candida albicans ORFs from SC5314 genomic DNA. PCR fragments were purified on MultiScreen-PCR 96-well filtration units (Millipore, Bedford, Mass.) and were eluted in spotting buffer (3 SSC [(1 SSC is 0.15 M NaCl plus 0.015 M sodium citrate] plus 0.01% SDS). Purified PCR products were spotted in duplicate on poly-L-lysine-coated slides with a MicroGrid II (BioRobotics, Cambridge, United Kingdom). Slides were postprocessed with succinic anhydride in 1-methyl-2-pyrrolidinone (http://www.microarrays.org/protocols.html).