title: Cyanidioschyzon merolae cell cycle
status: Public on May 08 2009
contact.city: Toshima-ku
contact.first: Takayuki
contact.last: Fujiwara
contact.email: 07rd003h@stu.rikkyo.ne.jp
contact.address: Nishi-ikebukuro3-34-1
contact.department: Life Science
contact.country: Japan
contact.institute: Rikkyo University
contact.phone: 081-03-3985-4592
organism: Cyanidioschyzon merolae
manufacture_protocol: Based on the annotated C. meroae genome, we ordered synthesis of the ORF-specific oligonucleotides for Sigam Genosys and searched a unique 50-mer sequence for each of the 4586 ORFs coding protein gene (>96% of the ORFs in the nucleus genome). In brief, 50-mer sequences were first selected from ORF sequences based on a set of criteria that included Tm of 75 ± 10 degrees Celsius. 98% of the ORFs-specific oligonucleotides were selected among 1.5 kb from the 3′ end of the ORF.  All the oligonucleotiodes were synthesized (Sigma Genosys) and spotted onto poly-lysine–coated glass slides by using a spotting machine. Spotted glass slides were post-processed according to Brown lab’s protocol (http://cmgm.stanford.edu/pbrown/) and used directly in hybridization or stored at room temperature in a dry cabinet for later use.