title: BMAP_HGPD
status: Public on Oct 13 2004
contact.city: Winnipeg
contact.first: Garrett
contact.last: Sorensen
contact.email: GSorensen@mts.net
contact.country: Canada
contact.institute: Canadian Science Centre for Human and Animal Health
contact.phone: (204) 789-7048
organism: Mus musculus
description: Libraries and clones derived from brain regions of adult mouse (strain c57BL6) were obtained from Research Genetics, Inc. The 11,136 clones represent expressed sequence tags (ESTs) from some 5,471 unique known genes and 5,665 mRNAs as yet uncharacterised, and form part of a library created as part of the Brain Molecular Anatomy Project (BMAP). Each insert from this BMAP 3 EST library was amplified by PCR. The cDNA was purified using Millipore multiwell purification plates (Millipore Inc.), and lyophilised PCR products were resuspended in 1 X Micro Spotting Solution Plus (TeleChem International, Inc.) at a concentration of 0.25-0.75 µg/µl. DNA was spotted onto CMT-GAPS coated glass slides (Corning) using Stealth micro-spotting pins (TeleChem International, Inc.).