title: LC Sciences miRPlants miRBase_10.0
status: Public on Oct 26 2011
contact.city: Shanghai
contact.first: Zaochang
contact.last: Liu
contact.email: zaochangliu@hotmail.com
contact.address: 2901 Beidi Rd., Minhang District
contact.department: Lab
contact.country: China
contact.institute: SAAS
contact.phone: 21-52230526
organism: Oryza sativa
manufacture_protocol: 1. 623 miRNAs based on Sanger miRBase Release 10.0 and 100 custom miRNA sequences are arrayed on the chip. Each probe is repeated 5 times.
  2. Multiple redundant regions are included.  Each region further comprises a miRNA probe region, which detects miRNA transcripts listed in Sanger miRBase Release 10.0.
  3. Multiple control probes are included in each chip.  The control probes are used for quality controls of chip production, sample labeling and assay conditions.
  4. Among the control probes, PUC2PM-20B and PUC2MM-20B are the perfect match and single-based match detection probes, respectively, of a 20-mer RNA positive control sequence that is spiked into the RNA samples before labeling.
  5. One may assess assay stringency from the intensity ratio of PUC2PM-20B and PUC2MM-20B, which is normally larger than 30.