title: BAMC-DCI GAS 12K ver1.0 [condensed version]
status: Public on Nov 01 2010
contact.city: San Antonio
contact.first: Jeffrey
contact.last: Livezey
contact.email: jeffrey.livezey@us.army.mil
contact.state: TX
contact.address: 3400 Rawley E Chambers Ave
contact.department: Clinical Investigation
contact.country: USA
contact.institute: Brooke Army Medical Center
organism: Streptococcus pyogenes M1 GAS
  Streptococcus pyogenes SSI-1
  Streptococcus pyogenes MGAS315
  Streptococcus pyogenes MGAS5005
  Streptococcus pyogenes MGAS6180
  Streptococcus pyogenes MGAS9429
  Streptococcus pyogenes MGAS2096
  Streptococcus pyogenes MGAS10750
description: The full array layout file provided as a supplementary file.
manufacture_protocol: Probe sets were 30-40mers in length, sense strand and were selected using a Combimatrix probe selection algorithm taking into account gene (>90% BLAST score) and serotype specificity, Tm, hairpins and GC content. Arrays were constructed using the CustomArray Synthesizer at Combimatrix. CustomArray uses a specially modified CMOS semiconductor to direct the molecular assembly of a specific sequence of DNA bases in response to a digital command. Each feature on the array (a microelectrode) is digitally addressed to selectively generate acid, by means of an electrochemical reaction which, in-turn, controls the detritylation reaction during phosphoramidite synthesis. A software program is used to control the microelectrodes, and thus, the detritylation pattern applied on the chip during synthesis. Seventy factory built in negative controls and 327 quality control probes are also included in the microarray.