title: 16S and 23S rRNA CodeLink Microarray
status: Public on Nov 05 2005
contact.city: Louvain-la-Neuve
contact.first: Laurent
contact.last: Eyers
contact.email: eyers@gebi.ucl.ac.be
contact.address: Place Croix du Sud 2/19
contact.department: Unit of bioengineering
contact.country: Belgium
contact.institute: Catholic University of Louvain
organism: unidentified
description: Probes targeting the 16S and 23S rRNA of various microorganisms (mainly Bacteria).
manufacture_protocol: The CodeLink microarray contained oligonucleotide probes deposited on CodeLink slides of uniformly coated hydrophilic polymer containing amine-reactive groups (Amersham Biosciences, Buckinghamshire, UK). Oligonucleotide probes were manufactured by Argonne National Laboratory and Qiagen, Inc., and approximately 6 fmol of each probe was printed in duplicate with a SpotBot arrayer (Telechem International). Probes were covalently attached to the slide via a 3' C7 primary amine linker. Probe attachment, slide hydration, and blocking were done as described by the slide manufacturer's instructions. The design of oligonucleotide probes targeting rRNA molecules was done as described previously (El Fantroussi et al. 2003, Appl. Environ. Microbiol. 69:2377-2382). CodeLink microarrays contained 83 different perfect match oligonucleotide probes (13-25 nucleotides in length) that were complementary to universal, domain, species, and phylogenetic group specific rRNA sequences.