title: SUCEST-FUN Sugarcane 44k v1.0
status: Public on Jul 31 2012
contact.city: São Paulo
contact.first: Milton
contact.middle: Yutaka
contact.last: Nishiyama Jr
contact.email: yutakajr@gmail.com
contact.state: São Paulo
contact.address: Av. Professor Lineu Prestes, 748
contact.department: Biochemistry
contact.country: Brazil
contact.web_link: http://sucest-fun.org
contact.institute: Universidade de São Paulo - USP
organism: Saccharum officinarum
description: CaneRegNet microarray (44k multiplex chip, agilent) was designed using an in-house developed method for empirical selection of best performing probes (60bp long oligomers) for each SAS (Sugarcane Assembled Sequence - Pre Selection Strategy based on gene function and categorization). Were designed two probes for each cluster SAS from SUCEST Project, one in the sense orientation and another one in the antisense orientation if available or one sense probe. The microarray contain 21902 oligo probes in sense and antisense orientation corresponding of a total of 14522 SAS.
  
  The ID column includes unique numeral id (for each 'spot' on the array). A different version of this platform with unique probe name in the ID column is assigned accession number GPL22278.
manufacture_protocol: We labeled, hybridized, and washed the arrays according to Agilent's Two-Color Microarray-Based Gene Expression Analysis(Quick Amp labeling) Protocol (version 5.7, March 2008)