title: MBPL human 30k P8
status: Public on Mar 20 2015
contact.city: Hsinchu
contact.first: Chaang Ray
contact.last: Chen
contact.email: d948504@oz.nthu.edu.tw
contact.address: 101, Section 2, Kuang-Fu Road
contact.department: Biomedical Engineering and Environmental Sciences
contact.country: Taiwan
contact.institute: National Tsing Hua University
organism: Homo sapiens
description: spotted DNA/cDNA
manufacture_protocol: Arrayer obtained from Dr. Patrick O. Brown MGuide II and ArrayMarker program version 2.3.2 (Dr. Joseph DeRisi, University of California, San Francisco) with 48 spotting pins (TeleChem).We control printing condition in temperature 25~26°C and Humidity about 49~51%, and printing on January 2009. cDNA clones were purchased from Incyte Genomics and 7334 sequence-verified clones were amplified by PCR.We purified these 7334 clones by isopropanol precipitation and used 3× saline-sodium citrate (SSC) as spotting solution. cDNA spotted on GAPS II coated slides (Corning Incorporated) coating of pure gamma amino propylsilane.