title: LC Human-Mouse-Rat miRNA Array
status: Public on Dec 31 2011
contact.city: Chicago
contact.first: Dauren
contact.last: Biyashev
contact.address: 300 E.Superior St.
contact.country: USA
contact.institute: Northwestern University
organism: Homo sapiens
  Mus musculus
  Rattus norvegicus
manufacture_protocol: Arrays are made based on µParaflo microfluidic technology (Atactic Technologies). On the µParaflo microfluidic chip, each detection probe consists of a chemically modified nucleotide-coding segment complementary to target microRNA (from miRBase, http://microrna.sanger.ac.uk/sequences/) or other RNA (control or customer defined sequences) and a spacer segment of polyethylene glycol to extend the coding segment away from the substrate. The detection probes are made by in situ synthesis using PGR (photogenerated reagent) chemistry.