title: NHRI Human 46K cDNA array
status: Public on Sep 01 2010
contact.city: Miaoli County
contact.first: Chieh Hua
contact.last: Lin
contact.email: mammer@nhri.org.tw
contact.address: 35, Keyan Road, Zhunan Town
contact.department: Division of Biostatistics and Bioinformatics
contact.country: Taiwan
contact.institute: National Health Research Institutes
organism: Homo sapiens
manufacture_protocol: The NHRI human cDNA microarray contains 46,657 cDNA clones (IMAGE consortium), representing approximately 26,000 unique Unigene clusters. We fabricated cDNA microarrays essentially following protocols as described by Michael B. Eisen and Patrick O. Brown with minor modification. Briefly, the purified cDNA products were resuspended in spotting solution containing 50% dimethyl sulfoxide. The final concentration of each probe was around 0.2 to 0.5 mg/ml. These probes were robotically arrayed onto aminosilane-coated UltraGAPS glass microarray slides (Corning) using a home-made robotic microarray spotter. In addition to the human cDNA probes, a series of control DNA probes from the Lucidea Universal ScoreCard (Amersham Bioscience) were also included for quality assessment of the microarray experiment. In total, there are 49,152 probes spotted on a single microarray slide.