title: PIQOR Immunology Microarray Mouse Antisense
status: Public on Sep 13 2007
contact.city: Cologne
contact.first: Bernhard
contact.last: Gerstmayer
contact.email: bernhard.gerstmayer@miltenyibiotec.de
contact.address: Stockheimer Weg 1
contact.country: Germany
contact.institute: Miltenyi Biotec GmbH
contact.phone: 0049-221-95048211
organism: Mus musculus
description: The PIQOR Immunology Microarray Mouse Antisense comprises 1076 genes (including six housekeeping genes and six controls).
manufacture_protocol: Defined 200-400 bp fragments of selected cDNAs were generated by RT-PCR, cloned and sequence-verified. Amplified and purified cDNA inserts were spotted in quadruplicate on PIQOR slides using a proprietary piezoelectric spotter. The PIQOR slides were coated in a 2 step process generating a surface which covalently binds DNA molecules.