title: Locust 20k 60-mer oligo array
status: Public on Dec 12 2012
contact.city: New Haven
contact.first: Dejian
contact.last: Zhao
contact.email: dejian.zhao@yale.edu
contact.state: CT
contact.address: 333 Cedar St
contact.department: Genetics
contact.country: USA
contact.institute: Yale School of Medicine
organism: Locusta migratoria
manufacture_protocol: Oligos are arrayed in Greiner 384-well flat-bottom plates. Each well contains 600 pmol of 70-mer oligo. Resuspend oligos in water to 20 uM and rearray 5 µL into 384-well, Genetix polystyrene V-bottom plates (cat# X6004). Allow Genetix plates to dry through passive water evaporation in a protected environment (e.g., chemical hood). Before printing, add 5 µL of 1X Printing Buffer to each well. This can be done the night before a print run is started. Seal plates with Corning seals. Incubate at 37°C for 30 minutes to aid resuspension of DNA. Shake plates near maximum rotational speed on flat-bed shaker for 1 minute. Centrifuge plates at 2000 rpm for 3 minutes. Remove seals and cover with plate lids. Place in appropriate location of plate cassette. This should be done with first plates just before print run is started to minimize evaporation time before printing. For second and third cassettes, wait until 30  Make sure plates rest behind both holding clips in the cassettes. Push plates back into the cassettes as far as they will go, putting them in the proper position for the server arm. After the print run is completed, allow plates to dry through passive evaporation in a protected environment. For each subsequent preparation of these plates for a print run, add water to the wells instead of sodium phosphate buffer.