title: USDA 9.6K avian intestinal intraepithelial lymphocyte cDNA microarray (AVIELA) version 1
status: Public on May 27 2009
contact.city: Beltsville
contact.first: Hyun 
contact.last: Lillehoj
contact.email: hyun.lillehoj@ars.usda.gov
contact.state: MD
contact.address: USDA-ARS, Bldg. 1040, BARC-East
contact.department: Animal Research Service
contact.country: USA
contact.web_link: http://www.usda.gov
contact.institute: US Department of Agriculture
contact.phone: 301-504-6170
organism: Gallus gallus
manufacture_protocol: The microarray was constructed from 9,668 EST clones selected from an intestinal IEL cDNA library. PCR products of the ESTs were dried and resuspended in 3X SSC, 0.01% SDS, followed by spotting in duplicate  onto MicroMax glass slides (Perkin Elmer, Boston, MA) using an OmniGrid Accent spotter (GeneMachines, San Carlos, CA).  The elements were organized into duplicated 24 subarrays, each as a 21 x 21 spot grid.  The PCR products were fixed by UV cross-linking, followed by assessment of spotting quality by staining with SYBR-Green II according to the manufacturer's protocol (Invitrogen, Carlsbad, CA).  Greater than 90% of the spots possessed an average diameter of 150-200 µm.