title: Rosetta Genomics human miRNA array v34 (condensed)
status: Public on Jul 01 2011
contact.city: Rehovot
contact.first: Einat
contact.last: Sitbon
contact.address: 10 Plaut St
contact.country: Israel
contact.institute: Rosetta Genomics
organism: Homo sapiens
description: ROSG hsa 720 v.34
  Compared to v33, the v34 design strengthened the probes for 4 miRs galfile v.34 by adding AG at their end. The strengthened probes are: hsa-miR-21, hsa-miR-374a, hsa-miR-365, hsa-miR-369-3p.
  The array probes were planned according to mirBase 9. The naming of the probes was updated to match miRbase 15.
  The Platform data table reflects a condensed representation of the array's triplicate features.
manufacture_protocol: Custom microRNA microarrays were spotted on Slide E coated microarray slides (Schott Nexterion, Mainz, Germany) ~650 DNA oligonucleotide probes representing microRNAs (Sanger database version 9 and additional microRNAs predicted and validated by Rosetta Genomics).Probes spotted in triplicate using the BioRobotics MicroGrid II microarrater (Genomic Solutions, Ann Arbor,MI) according to the manufacturer’s directions. Fifty-four negative control probes were designed using the sense sequences of different microRNAs. Two types of positive control were included in the experimental design: (i) synthetic small RNAs were spiked into each RNA sample before labeling to verify labeling efficiency; and (ii) probes for abundant small RNAs were spotted on the miRdicator™ array to validate RNA quality.