title: miRNA microarray
status: Public on Feb 01 2006
contact.city: Research Triangle Park
contact.first: Michael
contact.middle: D. 
contact.last: Mattie
contact.email: mdmattie@yahoo.com
contact.state: NC
contact.address: Five Moore Drive
contact.department: Genomics Research
contact.country: USA
contact.institute: GlaxoSmithKline
contact.phone: 919-483-5617
organism: Homo sapiens
manufacture_protocol: Printing, post-processing, and analysis of the microarrays was performed as described (www.microarrays.org). An oligonucleotide microarray was constructed containing all of the annotated human microRNA genes in the miRNA Registry (www.sanger.ac.uk/Software/Rfam/mirna/).  Oligonucleotide probes identical to the sense orientation of the mature human miRNA sequences were synthesized by Operon Biotechnologies (Huntsville, AL) and duplicate spotted in 3X SSC on Gold Seal microslides (Becton Dickinson, Bedford, MA) coated with poly-l-lysine using a linear servo arrayer at the UCSF Core Facility for Genomics and Proteomics.  Post-printing processing was performed using succinyl anhydride as described (http://microarrays.org).  Arrays were printed with probes consisting of a tandem repeat or dimer of the mature sequences.  At the time of printing the arrays contained all of the annotated human microRNA genes in the miRNA Registry as of November, 2004 (approximately 200).  A total of 14 negative control sequences were also printed on the array.  These sequences are reverse sequences of selected miRNAs.  We have not observed any binding of sequences to any of the negative controls.