title: NCSU_Metallosphaera sedula_2Karray_version1
status: Public on Apr 29 2008
contact.city: Raleigh
contact.first: Kathryne
contact.middle: Sherlock
contact.last: Auernik
contact.email: kpsherlock@yahoo.com
contact.state: NC
contact.address: Partners II, rm 3309, 849 Main Campus Drive
contact.department: Chemical and Biomolecular Engineering
contact.country: USA
contact.web_link: http://www.che.ncsu.edu/extremophiles/
contact.institute: North Carolina State University
organism: Metallosphaera sedula
manufacture_protocol: A DNA microarray was designed and constructed based on at least 2258 protein-coding sequences in the M. sedula genome obtained from the Department on Energy’s Joint Genomes Institute (http://genome.ornl.gov/microbial/msed). OligoArray 2.1 (Rouillard, 2003) was used to generate at least one 60-mer oligonucleotide probe sequence for each open reading frame. The probes were synthesized (Integrated DNA Technologies, IA), re-suspended in 50% DMSO, and printed onto Ultragap microarray slides (Corning, NY) using a QArrayMini arrayer (Genetix, UK). Each probe was spotted five times onto each array to fortify statistical analysis.