title: Meyerowitz Lab Arabidopsis Operon Array Version 4
status: Public on Apr 02 2010
contact.city: Bellaterra
contact.first: Jose Luis
contact.last: Riechmann
contact.email: joseluis.riechmann@cragenomica.es
contact.state: Barcelona
contact.address: Campus UAB - Edifici CRAG
contact.country: Spain
contact.institute: Center for Research in Agricultural Genomics
organism: Arabidopsis thaliana
description: This microarray is based on the Arabidopsis Genome Oligo Set Version 1.0 and on the Arabidopsis Genome Oligo Set Version 1.0 Upgrade (Operon, Alameda, CA). These sets consist of a total of 30,194 oligonucleotides.
manufacture_protocol: The oligonucleotides of the Arabidopsis Genome Oligo Set (Operon) were provided in 384-well plates. The dried DNA was resuspended in 15 ul of printing buffer (150 mM Na-phosphate pH 8.5) per well, resulting in an estimated DNA concentration of 40 uM. DNA was spotted onto poly-lysine coated glass slides with a MicroGrid II spotter (Biorobotics) using Microspot 10K quill pins (Biorobotics). After printing, the slides were processed and blocked following standard protocols.