title: Lallemand Health Solutions Human Immune Array
status: Public on Dec 31 2011
contact.city: Montreal
contact.first: Thomas
contact.middle: Allan
contact.last: Tompkins
contact.state: Qc
contact.address: 6100 Royalmount Avenue
contact.department: R&D
contact.country: Canada
contact.institute: Lallemand Health Solutions Inc.
organism: Homo sapiens
manufacture_protocol: Genes were selected from the KEGG data base from specific pathways. Gene specific long oligonucleotides sequences (70-mers) were recovered from the Human Exonic Evidence Based Oligonucleotide (HEEBO) database. The oligonucleotides were synthesized (IDT integrated technology Inc. IA, USA) at a normalized concentration of 25 mM and spotted onto Corning UltraGAPS Coated Slides using a PBI robot from Virtex. The Immune Array design was repeated twice per slide in six subgrid and each gene was in duplicate. The microarray slides were printed at the NRC Biotechnology Research Institute (Montreal, QC Canada) in the Microarray Laboratory