title: NimbleGen Geobacter sulfurreducens KN400-v1.0
status: Public on Jun 18 2013
contact.city: Amherst
contact.first: Jessica
contact.middle: Amber
contact.last: Smith
contact.email: jsmith@bio.umass.edu
contact.state: MA
contact.address: 203 Morrill Science Center IVN
contact.department: Microbiology
contact.country: USA
contact.institute: UMASS Amherst
contact.phone: 413-441-3403
organism: Geobacter sulfurreducens KN400
description: Custom 4x72k Gene Expression design for Geobacter sulfurreducens KN400 (accession number CP002031). 3328 CDS features were extracted from a GenBank file downloaded from NCBI on December 14th, 2011. 45-60mer probes were selected from the entire length of the sequences. 10 probes were selected for 3313 sequences. 15 sequences share all 10 probes with another sequence and are represented by exemplars. Probes were replicated 2 times on the array in containers BLOCK1-BLOCK2.  The remainder of the array was filled with randomly generated 45-60mer probes (GC=0.58, Tm=83, max. cycles=148).
  120104_Gsulf_JW_expr.ndf
  120104_Gsulf_JW_expr.ngd
manufacture_protocol: High-density DNA array prepared with Maskless Array Synthesizer (MAS) technology. See manufacturer's website at http://www.nimblegen.com/.