title: NIAID - Mmac
status: Public on Feb 11 2004
contact.city: Bethesda
contact.first: Michael
contact.last: Wilson
contact.email: mwilson@ambion.com
contact.state: MD
contact.address: 50 South Drive, Room 5509
contact.department: Division of Intramural Research
contact.country: USA
contact.web_link: http://madb.niaid.nih.gov/
contact.institute: National Institute of Allergy and Infectious Diseases
contact.phone: 301-496-4287
organism: Mus musculus
description: Printing by NIAID / Microarray Research Facility
  Surface type: non-adsorptive, polylysine-coated glass
  Attachment: covalent, (UV-treated polylysine)
  Array description: The custom microarrays are manufactured by the Microarray Research Facility of the National Institute of Allergy and Infectious Diseases (NIH/NIAID). Microarrays are printed with an Omnigrid robotic arrayer by Genomic Solutions (http://www.genomicsolutions.com ) that is fitted with a printhead and 32 SMP3 print tips from Telechem (http://www.arrayit.com ). The mouse microarray (Mmac) probe set is based on 70mer oligonucleotides from Qiagen's Mouse Genome Oligo Set V1.1 (http://oligos.qiagen.com/arrays/omad.php ).  A comprehensive gene list is also available at http://madb.niaid.nih.gov
  
  Oligonucleotide probes are distributed into 384-well polypropylene plates (Genetix, 384 micro array) and dissolved in 4XSSC print buffer at a normalized 25 micromolar concentration. The Mmac microarray spot densities are 190 microns (center-to-center).  The microarrays are printed on Fisher Premium microscope slides (cat# 12-544-1) coated with poly-L-lysine and post-processed according to the procedures on the Stanford website: http://cmgm.stanford.edu/pbrown/protocols/index.html .