title: CAT-30K Schizosaccharomyces-probes-str-ASM294v2
status: Public on Jun 30 2023
contact.city: Puebla
contact.first: Irma
contact.last: Herrera-Camacho
contact.email: irma.herrera@correo.buap.mx
contact.state: Puebla
contact.address: Av. San Claudio y 22 sur, Jardines de San Manuel
contact.department: Instituto de Ciencias
contact.country: Mexico
contact.institute: Benemérita Universidad Autónoma de Puebla
contact.phone: +52 2222295500
organism: Schizosaccharomyces pombe
manufacture_protocol: Probes specific to 5131 genes from Schizosaccharomyces pombe  were designed by MYcroarray, Ann Arbor, MI (now Daicel Arbor Biosciences, Ann Arbor, MI) using a proprietary version of the publicly available software OligoArray2.1. One specific probe was designed for each gene, 4345 probes were six technical replicates of each unique probe sequence and some probes had 5 technical replicates were included on each array.  45-mer oligonucleotide probes were synthesized in situ, not spotted, on standard size (25mm x 76mm) glass slides using RotoSynth Software Version 1.0. All probes were synthesized on amine-functionalized glass substrates such that the 3' end was covalently coupled to a spacer moiety, which was covalently linked to an amine. The 5’ end of the probe sequence was pushed away from the glass by synthesizing a lawn of poly-deoxythymidine spacers. Two identical 30K arrays were synthesized on each slide so that different samples could be applied independently to each array with the use of gasket slides during hybridization to segregate the replicate arrays. Arrays also included control probes for flour-labelled spike-in control oligos for assessing hybridization and washing stringency. https://arborbiosci.com/