title: Fundulus_192gene_metabolic_array
status: Public on Feb 18 2005
contact.city: Miami
contact.first: andrew
contact.last: whitehead
contact.email: awhitehead@rsmas.miami.edu
contact.state: FL
contact.country: USA
contact.institute: University of Miami, RSMAS
contact.phone: 305-361-4627
organism: Fundulus heteroclitus
description: Microarrays were printed using 192 cDNAs from a F. heteroclitus cardiac library encoding essential proteins for cellular metabolism.  These cDNAs were a subset of over 33,000 expressed sequences in our database, which all may be found at http://genomics.rsmas.miami.edu/funnybase/super_craw3/ (Oleksiak et al. 2001).  These 192 cDNAs were amplified with amine-linked primers and printed on 3-D Link Activated slides (Surmodics Inc., Eden Prairie, MN) using a SpotArray Enterprise piezoelectric microarray printer (PerkinElmer Life Sciences Inc., Boston, MA, USA) at Louisiana State University.  Slides were blocked following slide manufacturer protocols.  The suite of 192 amplified cDNAs was printed as a group in spatially separated replicates (6 replicates for 178 genes, and 4 replicates for 14 genes).  Four hybridization zones of these six replicate arrays were printed per slide, with each zone set separated by a hydrophobic barrier.
  Keywords = Fundulus
  Keywords = metabolism
  Keywords = populations
  Keywords = tissue-specific gene expression