title: NCI/ATC Mm-MEEBO 30.7k array
status: Public on Jul 21 2009
contact.city: Frederick
contact.first: Debra
contact.last: Tross
contact.email: trossd@mail.nih.gov
contact.state: MD
contact.address: 1050 Boyle St/567/203
contact.country: USA
contact.institute: NCI
organism: Mus musculus
description: Derived from GEO Platform GPL4200 03/25/09 - NCI/ATC Mm-MEEBO.
manufacture_protocol: Microarrays are printed with GeneMachines robotic arrayer from Genomic Solutions (http://www.genomicsolutions.com) that is fitted with a printhead containing 48 SMP2.5 printing pins from Telechem International (http://www.arrayit.com). The mouse long oligonuleotide probe set contains 38,467 optimized 70mers (plus NCI Spotplate #4 controls) obtained from Invitrogen (http://www.invitrogen.com/). The long oligonucleotide probes were purchased in 384-well polypropylene plates (Genetix, 384 microarray) and dissolved print buffer of sodium phosphate (150mM) at ph 8.5 and 4% sodium sulphate at a concentration of 20-30 uM. The microarray spots are printed with 160 micron column x 155 microns row spacing, and one spot is printed per probe per slide. The microarrays were printed on Corning Expoxide slides and post-processed according to the manufacturer's protocol.