title: Buck Institute Human 8k cDNA microarray
status: Public on Feb 17 2006
contact.city: Ottawa
contact.first: Douglas
contact.middle: Joseph
contact.last: Mahoney
contact.email: doug@mgcheo.med.uottawa.ca
contact.state: ON
contact.address: 401 Smyth Rd
contact.department: Apoptosis Research Center
contact.country: Canada
contact.institute: CHEO Research Institute
contact.phone: 613 738 3927
organism: Homo sapiens
manufacture_protocol: 8,432 randomly chosen cDNAs from a 40k Research Genetics human cDNA library were amplified under standard conditions (GeneAmp PCR System 9700) with the help of a volumetric robot (Tecan/Genesis, RSP150 and Tomtec/Quadra 384).  Amplified products were transferred to a 384-well plates, purified transferred to 384-well print plates (Genetix), dried and resuspended in print buffer (3xSSC, 1.5M Betaine).  Spotted cDNA microarrays were generated on glass slides (MWC epoxy) using an Omnigene (GeneMachines) microarrayer.  After printing, slides were incubated overnight at 50% humidity, UV irradiated (65 ujoules), and stored in a desicator until hybridization.