title: Toxoplasma gondii Chromosome 1b 1230000-1880000 tiling array
status: Public on May 22 2007
contact.city: Tampa
contact.first: Kami
contact.last: Kim
contact.email: kamikim.usf@gmail.com
contact.state: FL
contact.address: 3720 Spectrum Blvd, Suite 304
contact.department: Internal Medicine Medicine
contact.country: USA
contact.institute: University of South Florida
contact.phone: 813 974 5936
organism: Toxoplasma gondii
description: Tiling microarray encompassing .65Mb (nts 1230000-1880000) of Chromosome 1b, accession number AM055943.1.
manufacture_protocol: NimbleGen manufactures custom, high-density DNA arrays based on its proprietary Maskless Array Synthesizer (MAS) technology. The MAS system is a bench top, solid-state, high-density DNA array fabrication instrument comprised of a maskless light projector, a reaction chamber, a personal computer, and a DNA synthesizer. NimbleGen builds its arrays using photo-mediated synthesis chemistry with its MAS system.
  At the heart of the system is a Digital Micromirror Device (DMD), similar to Texas Instruments' Digital Light Processor (DLP), employing a solid-state array of miniature aluminum mirrors to pattern up to 786,000 individual pixels of light. The DMD creates "virtual masks" that replace the physical chromium masks used in traditional arrays.
  These "virtual masks" reflect the desired pattern of UV light with individually addressable aluminum mirrors controlled by the computer. The DMD controls the pattern of UV light projected on the microscope slide in the reaction chamber, which is coupled to the DNA synthesizer. The UV light selectively cleaves a UV-labile protecting group at the precise location where the next nucleotide will be coupled. The patterns are coordinated with the DNA synthesis chemistry in a parallel, combinatorial manner such that up to 786,000 unique probe features are synthesized in a single array.