title: Porcine oligonucleotide microarray version 4 (POM4) (Condensed version)
status: Public on Nov 08 2008
contact.city: Frederiksberg
contact.first: Jayda
contact.last: Siggers
contact.email: jlac@life.ku.dk
contact.state: C
contact.address: Roligehdsvej 30
contact.department: Human Nutrition
contact.country: Denmark
contact.institute: University of Copenhagen
contact.phone: +45 3533 2698
organism: Sus scrofa
manufacture_protocol: 1. This microarray is holding 384 different 60-70mer oligonucleotide probes representing more than 200 different immune related porcine genes and 8 synthetic array control oligonucleotide probes (ArrayControl)
  2. The porcine target sequences used for oligo design were obtained from public databases (NCBI, National Center for Biotechnology Information, GenBank (http://www.ncbi.nlm.nih.gov/) and the Gene Index Project (http://compbio.dfci.harvard.edu/tgi/tgipage.html)
  3. Probes were designed using Oligowiz (http://www.cbs.dtu.dk/services/OligoWiz2/)
  4. Probes were synthesized at DNA Technology A/S (Århus, Denmark)
  5. Probes were diluted to 50uM in 50% DMSO while array controls were diluted to 30uM in 50% DMSO
  6. Probes were spottet on Corning UltraGAPS Coated Slides using a VersArray Chip Writer Pro, software version 2.1.1., equipped with 8 SMP3 pins (TeleChem International, Inc)
  7. Oligonucleotides were spottet in tripilcate in 8 sub-arrays each holding 10 x 15 spots, these 8 sub-arrays were repeated 3 times vertically on the slide.
  8. After printing , slides were dried, UV cross-linked and stored in a vacuum desiccator at room temperature until use.
  9. Prior to hybridization the slides were pre-hybridized according to manufactures protecol