title: AccuTarget Human qPCR Screening (Pathway No. 60: Glucose Metabolism)
status: Public on Sep 22 2025
contact.city: Cheongju
contact.first: Hyo Youn
contact.last: Jo
contact.email: hyoyun05@chungbuk.ac.kr
contact.address: 1, Chungdae-ro, Seowon-gu, Cheongju-si, Chungcheongbuk-do
contact.department: Veterinary medicine
contact.country: South Korea
contact.institute: Chungbuk National University
contact.phone: +8201076625178
organism: Homo sapiens
description: AccuTarget Human qPCR Screening Kit (Pathway No. 60: Glucose Metabolism)
  qPCR-based gene expression profiling using SYBR Green detection
manufacture_protocol: Lyophilized forward and reverse primers were designed according to MIQE guidelines and provided in 96-well plate format. Each well contains one primer set for a glucose metabolism-related target gene or a housekeeping gene. Primers were resuspended with nuclease-free water before use, and qPCR was performed with AccuPower? 2X GreenStar? qPCR Master Mix (Bioneer) under a two-step protocol (95 °C pre-denaturation, followed by 45 cycles of 95 °C denaturation and 58 °C annealing/extension).
  https://www.bioneer.co.kr/literatures/manual/GeneExpressionAnalysis/AccuTarget_qPCR_Screening_Kit.pdf