title: Chr. Hansen A/S_Lactococcus lactis subsp. lactis_2208_v1.0
status: Public on Jun 22 2007
contact.city: Cadorago
contact.first: Martin
contact.middle: Bastian
contact.last: Pedersen
contact.email: martinbastianpedersen@gmail.com
contact.state: CO
contact.address: Via Manzoni 29
contact.department: R&D
contact.country: Italy
contact.institute: Sacco Srl
organism: Lactococcus lactis subsp. lactis
manufacture_protocol: DNA oligos were purchased from Bioneer Corporation at 50nmol synthesis scale with BioRP purification and in lyophilized state (Daedeok-gu, Daejeon, South Korea). Oligos were dissolved at 10uM in 50% DMSO and printed in 4 replicates on UltraGAPS slides (Corning B.V., Schiphol-Rijk, The Netherlands) using a Genpak ARRAY 21 arrayer (Genetix Ltd., Hampshire, United Kingdom) with 16 SMP3 pins, (TeleChem International Inc., Sunnyvale, CA) giving a spot diameter of 100-120mm. Washing was performed between each print cycle for 20s in water followed by 20s in 70% ethanol. The relative humidity was kept at 48 ± 5% by placing a water bath at the appropriate temperature below the air inlet of the arrayer. The oligos were cross-linked to the surface using a Stratalinker 1800 UV Crosslinker set at 70mJ/cm2 (Stratagene). Printed arrays were stored in aluminium pouches with five arrays each and silica gel to remove humidity.