title: Irradiated Mouse liver array
status: Public on Oct 20 2007
contact.city: sendai-city
contact.first: Taisuke
contact.last: Baba
contact.state: Miyagi
contact.address: seiryo-machi 4-1
contact.country: Japan
contact.institute: Tohoku Univ. IDAC
organism: Mus musculus
manufacture_protocol: 1.  Oligos are arrayed in Genetix 384-well V-bottom plates. Each well contains 300 pmol of 60-mer oligo.
  2. Resuspend oligos in 30-percent DMSO to 30 uM and rearray 10 uL into 384-well, Genetix polystyrene V-bottom plates (cat# X6004).
  3. After printing, rehydrate arrays by holding slides ( array side down) over a bath of hot double distilled H2O (95-C) for approximately 5 sec until a light bapor film is observed across the slide.
  4. Plate to dry on a 100-C hot plate for approximately 5 to 10 sec.
  5. Crosslinked DNA to the array slide using a UV crosslinker (150mJoules).
  6. Incubate at 42-C for 30 minutes by 5XSSC, 0.1-percent SDS, 0.1mg/mL BSA 25-percent formamide.
  7. Washing to remove the SDS completely.
  8. Centrifuge plates at 2000 rpm for 3 minutes.