title: Custom Salmonella LT2 and Tennessee Strain GE 4x44K (030869)
status: Public on Jan 01 2013
contact.city: Bedford Park
contact.first: Haiping
contact.last: Li
contact.state: IL
contact.address: 6502 S. Archer Rd
contact.department: CFSAN/OFS/Moffett Center
contact.country: USA
contact.institute: Food and Drug Administration
organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  Salmonella enterica subsp. enterica serovar Tennessee
description: Agilent 60-mer SurePrint technology: a proprietary non-contact industrial inkjet printing process, in which oligo monomers are deposited uniformly onto specially-prepared glass slides. This in situ synthesis process prints 60-mer length oligonucleotide probes, base-by-base, from digital sequence files. The precise inkjet process enables the delivery of extremely small, accurate volumes (picoliters) of the chemicals to be spotted.
manufacture_protocol: Standard phosphoramidite chemistry used in the reactions allows for very high coupling efficiencies to be maintained at each step in the synthesis of the full-length oligonucleotide. Precise quantities are reproducibly deposited “on the fly.” This engineering feat is achieved without stopping, to make contact with the slide surface and without introducing surface-contact feature anomalies, resulting in consistent spot uniformity and traceability. In addition, multiple microarrays can be printed simultaneously in a single manufacturing run.