title: Agilent-044345 UniversityPadova_Mytilus galloprovincialis_8x60K
status: Public on Dec 27 2014
contact.city: Legnaro
contact.first: Massimo
contact.last: Milan
contact.email: massimo.milan@unipd.it
contact.state: PD
contact.address: Viale dell'Università 16
contact.department: Dept. of Department of Public Health, Comparative Pathology,and Veterinary Hygiene
contact.country: Italy
contact.institute: University of Padova
contact.phone: +39 0498272941
organism: Mytilus galloprovincialis
description: Roche 454 sequencing of hemocytes, oocytes, larval stages (trochophores, veliger, pediveliger, settled larvae, one moth seed) and adult tissues (gills, foot, mantle, muscle, digestive gland, gonads) have been performed. A total of 1,325,571 reads have been obtained and assembled through MIRA3 (default parameters). Assembly produced a total of 103,995 transcripts. A preliminary annotation was obtained for 43,218 contigs (41.5%) through Blast similarity searches conducted against several protein databases (e-value 1 E-5). Probe design was carried out using the Agilent eArray interface (https://earray.chem.agilent.com/earray/), which applies proprietary prediction algorithms to design 60-mer probes. A total of 59,971 out of 59,997 probes were successfully obtained, representing 50,680 different M. galloprovincialis contigs.
manufacture_protocol: See SurePrint Technology at manufacturer's web site http://agilent.com/