title: NCI/LMT Mm_FCRF_UniGEM2
status: Public on Oct 27 2004
contact.city: Frederick
contact.first: Lisa
contact.middle: M
contact.last: Gangi
contact.email: gangil@pop.nci.nih.gov
contact.state: MD
contact.address: 915 Tollhouse Ave
contact.department: LMT-Frederick
contact.country: USA
contact.institute: NCI
contact.phone: 301-846-6296
organism: Mus musculus
description: Printing by the NCI-Frederick/LMT Microarray Research Facility
  Surface type: non-adsorptive, polylysine-coated glass
  Attachment: covalent, (UV-treated polylysine)
  Array description:  The custom arrays are manufactured by the NCI Frederick Laboratory of Molecular Technology Microarray Facility (NIH/NCI/SAIC/LMT).  Microarrays are printed with BioRobotics TAS II robotic arrayer from Genomic Solutions (http://www.genomicsolutions.com) that is fitted with a printhead containing 32 SMP3 printing pins from Telechem International (http://www.arrayit.com).  The mouse cDNA probe set is based on 9984 PCR products from Incyte's mouse UniGEM 2.0 library (http://www.incyte.com).  The set includes 9234 Unique GenBank Accessions; 8882 mapped into Mm UniGene Build #1201; 8656 Unique UniGene Clusters; 3463 EST clusters; 5193 "Named" clusters. The cDNA probes are distributed into 384-well polypropylene plates (Genetix, 384 micro array) and dissolved in 3XSSC print buffer at a concentration of 50-100 ug/ml.  The microarray spot densities are 235 microns (center-to-center), and one spot is printed per probe per slide.  The microarrays are printed on Fisher Premium microscope slides (cat# 12-544-1) coated with poly-L-lysine and post-processed according to the procedures on the NCI website http://nciarray.nci.nih.gov/ .