title: Mycobacterium tuberculosis AROS, Operon Biotechnologies, Inc.
status: Public on May 03 2008
contact.city: Aurora
contact.first: Ryan
contact.middle: William
contact.last: Honaker
contact.state: CO
contact.address: 12800 E. 19th Ave.
contact.department: Microbiology
contact.country: USA
contact.institute: University of Colorado Denver
organism: Mycobacterium tuberculosis CDC1551
  Mycobacterium tuberculosis H37Rv
manufacture_protocol: 1.Oligos are arrayed in Whatman 384-well V-bottom plates. Each well contains 600 pmol of 70-mer oligo.
  2. Oligos are suspended in 3xSSC to 20 uM and centrifuged at 500 rpm for 2 minutes.
  3. Plates are stored at 4oC overnight.
  4. Incubate at RT for 30 minutes to aid resuspension of DNA.
  5. Centrifuge plates at 500 rpm for 2 minutes.
  6. Remove seals and cover plates with lids.
  7. Print
  8. After completion of print, allow slides to dry through passive evaporation in a protected environment.