title: University of Alberta MAF Operon E. coli Genome Oligo Set Version 1.0 21k
status: Public on Mar 14 2008
contact.city: Jefferson
contact.first: Christopher
contact.middle: A
contact.last: Elkins
contact.email: chris.elkins@fda.hhs.gov
contact.state: AR
contact.address: 3900 NCTR Drive
contact.department: Microbiology
contact.country: USA
contact.institute: FDA National Center for Toxicological Research
contact.phone: 870-543-7547
organism: Escherichia coli
manufacture_protocol: 3x6K E. coli microarray on Corning epoxide slides.
  Each E. coli slide contains 5,978 oligos and a control set of 12 positive and 12 negative controls in a 4 by 4 block of sub-arrays which are printed in triplicate filling the slide surface with a total of 19086 spots printed at 200 um spacing in both the horizontal and vertical direction.
  The oligos are dissolved to a final concentration of 18.75 uM in 150 mM NaPO4 buffer (pH 8.7) prior to printing.
  The Operon E. coli Genome Oligo Set Version 1.0 consists of 5,978 70mer probes representing three strains of E. coli. The respective numbers of open reading frames represented in the array are as follows: 4,289 ORFs in K12, 5,029 ORFs in O157:H7 (EDL933), and 4,836 ORFs in O157:H7 (Sakai). All 5,978 probes are designed within known and predicted ORFs. For this probe set design, Operon employed state-of-the-art methods and proprietary software.
  All probes are Tm normalized to 75oC (+/- 5oC) and have a 5' amino linker.