title: GAP_M_Ia
status: Public on Jan 01 2005
contact.city: Lynchburg
contact.first: Michael
contact.middle: S
contact.last: Price
contact.email: msprice2@liberty.edu
contact.state: VA
contact.address: 1971 University Blvd.
contact.department: Biology & Chemistry
contact.country: USA
contact.institute: Liberty University
contact.phone: 434-592-7277
organism: Aspergillus flavus
description: PCR products were generated using approximately 5 ng plasmid DNA isolated from unique cDNA clones. T3 and T7 primers were used to generate amplicons. PCR products were then purified by isopropanol precipation followed by a 70% ethanol wash. The presence of amplicons was verified by agarose gel electrophoresis.
  PCR products at approximately 250 ng/ul in 50% DMSO were spotted in triplicate onto Corning GAPSII slides (Corning, NY) using an Affymetrix 417 Arrayer (Santa Clara, CA). Spots were allowed to air dry before being fixed following manufacturer’s recommendation.
  Keywords = aspergillus
  Keywords = aflatoxin