title: ORFs of Cryptococcus neoformans var. grubii (serotype A, H99)
status: Public on Jan 09 2007
contact.city: San Francisco
contact.first: Cheryl
contact.last: Chun
contact.email: jessica.brown@ucsf.edu
contact.state: CA
contact.address: 600 16th St
contact.country: USA
contact.web_link: http://cryptogenome.ucsf.edu
contact.institute: UC San Francisco
contact.phone: 415-514-0596
organism: Cryptococcus neoformans
manufacture_protocol: Gene models for serotype A strain H99 were developed using BLAST in combination with the gene models previously predicted by TIGR and Stanford centers for serotype D strains JEC21 and B-3501.  Based on these gene models, 70-mer oligos were designed using the program ArrayOligoSelector (Bozdech etal, DeRisi Lab, UCSF).  The oligonucletodies were printed on polylysine-coated glass microscope slides using a custom robot and silicon tips at UC San Francisco's Center for Advanced Technology.