title: Human 17K cDNA-GeneTrack
status: Public on Feb 01 2008
contact.city: Seoul
contact.first: DONG HYUK
contact.last: KI
contact.email: dki@ic.sunysb.edu
contact.address: sinchon-dong 134
contact.country: South Korea
contact.institute: NBRC
contact.phone: 82-2-2228-8047
organism: Homo sapiens
manufacture_protocol: 1.  cDNAs are amplified in Sorenson 96 well PCR plate (cat#21970) with 50 ul reaction volume and purified PCR products using Sephadex G-50 Fine (Amersham Biosciences, cat#17-0573-02).
  2. Transfer purified PCR products to Nunc 96 well V-bottom plates (cat#442587) and allow plates to dry through passive water evaporation in a protective environment (e.g., chemical hood).
  3. Resuspend cDNAs in 50% DMSO to 100-500 ng/ul (total volume 12 ul) and Seal plates with Corning seals
  4. Incubated plates at 60oC for 30 minutes to aid resuspension of cDNA and rearray 10 ul into 384-well, Genetix polystyrene V-bottom plates (cat# X6004). This can be done the night before a print run is started.
  5. Seal plates with Corning seals.
  6. Centrifuge plates at 2000 rpm for 3 minutes.
  7. Remove seals and cover with plate lids. Place in appropriate location of plate cassette. This should be done with first plates just before print run is started to minimize evaporation time before printing. For second and third cassettes, wait until 30 minutes before next cassette is needed to begin centrifugation.
  8. Make sure plates rest behind both holding clips in the cassettes. Push plates back into the cassettes as far as they will go, putting them in the proper position for the server arm.
  9. After the print run is completed, back plates to 4oC refrigerater.