title: ICAT Humulus lupulus 3.5K array version 1
status: Public on Jul 10 2008
contact.city: Lisboa
contact.first: Ana
contact.middle: Margarida
contact.last: Fortes
contact.email: margafortes@yahoo.com
contact.address: Campus da FCUL
contact.department: Plant Biology
contact.country: Portugal
contact.institute: BIOFIG_FCUL
contact.phone: +351 21 7500382
organism: Humulus lupulus
description: Technical Details of Array Design and Spotting
  Platform type: spotted glass array coated with Poly-L-lysine
  Microarrayer: ChipWriter Compact System (Biorad)
  Pins: 12 printing pins (Telechem International, Sunnyvale, CA, USA)
  Pin configuration: 2 x 6
  Number of repeats: spots are repeated once on the same subgrid in the same order. Last plate contains only printing solution.
  Printing plates: 36 x 2 (96 wells, one plate contains only printing solution)
  Printing scheme: 12 subgrids, each subgrid with 24 columns and 24 rows
  Distance between spots: 320μm
  Humidity: 40%
  Temperature: 22ºC
  This is a condensed version of the physical array, 7K. The full array linked as a supplementary file.
manufacture_protocol: Amplified cDNA inserts were purified with MultiScreen-PCR plate (Millipore), transferred to printing plates (Microtiter V plates, Sigma), resuspended in 50% dimethylsulphoxide, 0,2 % SSC and spotted in duplicate onto Poly-L lysine treated glass slides using VersArray ChipWriter Compact® (Bio-Rad Lab., Hercules, CA, USA). After printing, cross-linking was performed with heat and UV according to the protocol from Vodkin laboratory (http://soybeangenomics.cropsci.uiuc.edu/).
  Pins: 12 printing pins (Telechem International, Sunnyvale, CA, USA)
  Pin configuration: 2 x 6
  Number of repeats: spots are repeated once on the same subgrid in the same order.
  Printing plates: 36 x 2 (96 wells, one plate contains only printing solution) Printing scheme: 12 subgrids, each subgrid with 24 columns and 24 rows