title: Campylobacter jejuni 6K whole-ORF array v2.0
status: Public on Jul 31 2009
contact.city: East Lansing
contact.first: Linda
contact.middle: S.
contact.last: Mansfield
contact.email: mansfie4@cvm.msu.edu
contact.state: MI
contact.address: 181 Food Safety Bldg.
contact.department: Microbiology and Molecular Genetics
contact.country: USA
contact.institute: Michigan State University
contact.phone: 517-884-2027
organism: Campylobacter jejuni
description: Array includes ORF coverage from array "Campylobacter jejuni 6.5Kf whole-ORF array v1.0 (GPL7254) plus 46 additional ORFs amplified from strain 11168 using primers designed at Michigan State University.  Also an additional 883 ORFs were amplified from 8 other sequenced C. jejuni strains, primers also manufactured at Michigan State University
manufacture_protocol: 1. First set of Primers were designed at Wayne State University by Jodi R. Parrish, furnished to Michigan State University and are described in “High-Throughput Cloning of Campylobacter jejuni ORFs by in Vivo Recombination in Escherichia coli”, Journal of Proteome Research, Parrish et al., 2004 3(3):582-6.
  2. Primers for additional 46 ORFs from C. jejuni 11168 and 883 ORFs from 8 other C. jejuni strains were manufactured at Michigan State University
  3. PCR products were amplified from genomic DNA from Campylobacter jejuni 11168 or plasmid DNA obtained from C. jejuni 81-176.  Products were ethanol precipitated, resuspended in 3X SSC, 0.005% SDS print buffer, and run on gels for quality control.
  4. 16 pin replicate arrays were printed on Corning UltraGAPS slides using a GenMachines Omnigrid spotting robot and Telechem Chipmaker 3 pins.
  5. Each slide contains every spot in duplicate.