title: UIUC Bos taurus 13.2K 70-mer oligoarray (condensed)
status: Public on Oct 26 2009
contact.city: Tjele
contact.first: Kasey
contact.middle: M
contact.last: Moyes
contact.email: kasey.moyes@gmail.com
contact.address: Blichers Allé 20, P.O. BOX 50
contact.department: Animal Health, Welfare, and Disease
contact.country: Denmark
contact.institute: University of Aarhus
contact.phone: 004527285309
organism: Bos taurus
description: The 13,257 oligos used in this microarray were obtained from normalized and subtracted cattle placenta and spleen cDNA libraries and based upon the earlier cDNA array platform GPL2864 (Everts et al., 2005 Vet. Immunol. Immunopathol. 105:235-245) and subtracted cDNA libraries created from embryonic (day 36 and day 64) and extra-embryonic (day 14 to 25) tissues (NCBI libraries 15993,15993, and 17188). Positive controls include beta actin (ACTB), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and hypoxanthine phosphoribosyltransferase (HPRT). Exogenous spiking controls are the soybean genes chlorophyll ab binding protein (CAB), Rubisco small chain 1 (RBS1), and major latex protein (MSG). Negative controls are Cot1 DNA, genomic DNA, spotting buffer, poly-A, and water. All 70-mer oligos were printed in duplicate on the array.  Keywords: oligo, cow, placenta, bovine, embryo, microarray
  Platform GPL8776 is a condensed version of Platform GPL2853 ("UIUC Bos taurus 13.2K 70-mer oligoarray").
manufacture_protocol: 70-mer oligos were printed in 150 mM Phosphate buffer at 20 uM concentration.