title: NMC Homo sapiens 21K Hs-OperonV2
status: Public on Sep 30 2007
contact.city: Oslo
contact.first: Nur
contact.last: Duale
contact.address: Lovisenbergg. 8, P.O.Box 4404 Nydalen,
contact.department: Division of Climate and Environment Health
contact.country: Norway
contact.institute: Norwegian Institute of Public Health
organism: Homo sapiens
description: •Printing plates: 384 well low profile plates from Genetix (Cat# X60004)
  •Spotting conditions: 20–22°C and at 45–55% RH.
  •Slide post-processing: Slides are stored in a desicator cabinet to dry for 24-48 hours after spotting, and UV-crosslinked at 800 mJ/cm2
  (active side up) using a UV-Stratalinker 2400 from Stratagene Inc. The arrays are stored in Corning slide boxes before use.
manufacture_protocol: Array Production Details:
  •Spotting Instrumentation: MicroGrid II arrayer (model# 600),
  MicroSpot pin tool (Cat# BR001482),
  MicroSpot 10 K Split pins (Cat# BR001489),
  BioRobotics (see the description for the MicroGrid arrayer at the Genomic Solutions website)
  •Slide substrates: CMT UltraGAPS slides (25x75 mm) , Aminosilane coating (Corning Cat# 40016)
  •Oligonucleotide consentration: 40 μM (600 pmol oligonucleotide dissolved in 15 μL 50% DMSO)
  •Spotting buffer: 50% Dimethyl Sulphoxide (DMSO), (Sigma Cat# D-8418)