title: Human Unigene3.1 cDNA Array 37.5K v1.0
status: Public on Dec 09 2005
contact.city: Heidelberg
contact.first: Ruprecht
contact.last: Kuner
contact.address: Im Neuenheimer Feld 460
contact.department: Molecular Genetics
contact.country: Germany
contact.institute: German Cancer Research Center and National Center of Tumor Diseases
organism: Homo sapiens
description: The human Unigene3.1 cDNA Array represents 37530 cDNA clones provided by "Deutsches Ressourcenzentrum fuer Genomforschung (RZPD)"
manufacture_protocol: The microarrays carried 37,530 human cDNA clones (human UniGene Set RZPD3.1, German Resource Center for Genome Research, Berlin, Germany). DNA fragments were amplified by PCR and spotted on epoxysilane glass slides (Quantifoil, Jena, Germany) using the VersArray ChipWriter Pro System (Biorad, Munich, Germany) and SMP3 pins (Telechem, Sunnyvale, CA, USA). Spotting was performed in the division of Molecular Genome Analysis with a
  spacing of 28x29 Spots in 4x12 Subarrays (top-down to left-right). After spotting, microarrays were rehydrated, and the DNA was denatured with boiling water prior to washing with 0.2% SDS, water, ethanol, and isopropanol. The arrays were dried with air pressure.