title: MacLeod custom equine cartilage cDNA microarray
status: Public on Nov 09 2008
contact.city: Lexington
contact.first: Michael
contact.middle: J.
contact.last: Mienaltowski
contact.email: M.Mski@uky.edu, mmienalt@health.usf.edu
contact.state: KY
contact.address: 108 Gluck Equine Research Center
contact.department: Veterinary Science
contact.country: USA
contact.institute: University of Kentucky
contact.phone: 8592574757
organism: Equus caballus
description: The 9,322 cDNAs spotted on this microarray were obtained from a normalized and subtracted cDNA library generated from the total RNA of articular cartilage from a 15-month old equine Thoroughbred gelding.   In addition, cDNAs from 28 plasmids used within the lab were spotted; 2 plasmids have been submitted to GenBank.  Positive control spots include Elongation Factor Tu (EFTu) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Negative control spots are the soybean genes chlorophyll ab binding protein (CAB - SPOT_ID: ABbinding) and Rubisco small chain 1 (RBS1 - SPOT_ID: Rubisco). Other spots might be identified as empty of buffer.   Keywords: cDNA, equine, articular cartilage, microarray
manufacture_protocol: The cDNAs were spotted in 3x SSC buffer supplemented with 1.5 M betaine