title: CGH-SANGER 5K 2
status: Public on Jul 31 2006
contact.city: Leuven
contact.first: Rekin's
contact.last: Janky
contact.email: Nucleomics.Bioinformatics@vib.be
contact.address: Herestraat 49 Box 816
contact.department: Nucleomics Core
contact.country: Belgium
contact.institute: VIB
organism: Homo sapiens
description: The array contains 5088 distinct BAC and PAC sequences at an average resolution of 1Mb. The bacs were printed in 2 replicates.
  3490 BACS are from the Sanger institute. 1152  BACS were provided by K.U.Leuven Department CME (Belgium).
  and 96 BACS are from the Human 32K BAC rearray of CHORI BACPAC resources.
  The bacs were amplified by DOP - PCR.
  In total the array design contains 10080 features printed in a layout of
  12 meta rows, 4 meta columns, 14 rows per block and 15 columns per block.
manufacture_protocol: Equipment:
  1. Lucidea microarray spotter (Amersham Biosciences, Buckinghamshire, UK).
  2. 384 microtiter plates (Amersham Biosciences, Buckinghamshire, UK).
  3.  Motorola slides
  Printing protocol:
  1. Set spotter settings : 55% humidity, column pitch = 272 um, row pitch = 272 um.
  2. Make sure room temperature is between 20-24 degrees Celsius.
  3. Place microtiter plates in the plate hotel with A1 in upper right corner.
  4. Place 75 slides on the slide-deck and perform 2 manual pin-washes.
  5. Start spotting early in the morning and stop spotting procedure at end of the day and remove pin set. Leave spotter overnight with vaccuum and humidity settings on. Continue spotting the next morning until all plates are printed.
  6. Slides can be stored for at least 6 months in a dry-cabinet.