title: Massively Parallel Signature Sequencing - MPSS (classic method)
status: Public on Nov 29 2005
contact.city: Hayward
contact.first: Lynx
contact.last: Lynx
contact.email: tvasicek@lynxgen.com
contact.state: CA
contact.address: 25861 Industrial Blvd.
contact.country: USA
contact.web_link: http://www.lynxgen.com/wt/tert.php3?page_name=mpss
contact.institute: Lynx Therapeutics, Inc.
contact.phone: 510-670-9300
organism: Homo sapiens
description: Sequencing from the 3' most DpnII site from mRNA using Lynx's MPSS technology.
  MPSS allows a comprehensive survey of all expressed transcripts in a cell or tissue, without apriori knowledge.
  This dataset was annotated using a combination of LocusLink, Unigene (build 171) and the latest human build as provided from UCSC (hg16).
  Annotation Classe Table:
  Virtual Signature Class    mRNA Orientation                        Poly-Adenylation Features     Position
  0                          Either - Repeat Warning                 Not applicable                Not applicable
  1                          Forward Strand                          Poly-A Signal, Poly-A Tail    3' most
  2                          Forward Strand                          Poly-A Signal                 3' most
  3                          Forward Strand                          Poly-A Tail                   3' most
  4                          Forward Strand                          None                          3' most
  5                          Forward Strand                          None                          Not 3' most
  6                          Forward Strand                          Internal Poly-A               Not 3' most
  11                         Reverse Strand                          Poly-A Signal, Poly-A Tail    5' most
  12                         Reverse Strand                          Poly-A Signal                 5' most
  13                         Reverse Strand                          Poly-A Tail                   5' most
  14                         Reverse Strand                          None                          5' most
  15                         Reverse Strand                          None                          Not 5' most
  16                         Reverse Strand                          Internal Poly-A               Not 5' most
  22                         Unknown                                 Poly-A Signal                 Last before signal
  23                         Unknown                                 Poly-A Tail                   Last before tail
  24                         Unknown                                 None                          Last in sequence
  25                         Unknown                                 None                          Not last
  26                         Unknown                                 Internal Poly-A               Not 3' most
  1000                       Unknown- Derived from Genomic Sequence  Not applicable                Not applicable
  Keywords = MPSS, mRNA sequencing, tags