title: EcoArrayBass-4x44K-001 [ProbeName version]
status: Public on Aug 31 2012
contact.city: Alachua
contact.first: Barbara
contact.middle: Joan
contact.last: Carter
contact.email: bcarter@appliedfoodtechnologies.com
contact.state: FL
contact.address: 12085 Research Drive
contact.department: R&D
contact.country: USA
contact.institute: Applied Food Technologies
organism: Micropterus salmoides
  Micropterus dolomieu
description: This is the ProbeName version of GEO Platform GPL7799
  Our bass microarray contains genes for both largemouth (Micropterus salmoides) and smallmouth (Micropterus dolomieu) bass.  It was constructed by assembling over 40,000+ sequences to identify non-overlapping, unique sequences (singlets and contigs) in both species.  These data include our own proprietary sequences obtained from suppressive subtraction hybridizations and library construction plus sequence information licensed from the University of Florida.  Homology searches (Blast) were performed on these sequences in order to provide annotation (gene name) and ontology (gene function) information where present.  A total of 19,308 contigs were assembled.  Probes, 60-bases in length, were designed using Agilent’s web-based E-Array program.  The content of our bass microarray is located on our web site and contains 19,308 sequences.
manufacture_protocol: EcoArray selected Agilent Technologies as the manufacturer of our microarrays for environmental toxicology.   Probes are synthesized in situ using Agilent’s inkjet based “SurePrint” technology.  These arrays are manufactured on superior quality 1” x 3” (24.2 mm x 76.2 mm) treated glass slides for overall excellent performance.  This process minimizes variability across each production lot (batch to batch) and provides the highest consistency in slide-to-slide comparisons.  These glass slide microarrays are designed for either a one- or two-color  (cyanine-3 and cyanine-5) labeling reaction, the most reliable and sensitive methods for detecting differentially expressed genes.  Agilent's Feature Extraction software, when used with a high resolution (5 micron) scanner, provides QA metrics for each indivdual microarray, ensuring quality results.  Each slide contains four individual 44K arrays.