title: NCSU_chicken_JS2 (312 oligos)
status: Public on Jan 31 2008
contact.city: Raleigh
contact.first: Jean
contact.middle: Eduardo
contact.last: de Oliveira
contact.state: NC
contact.address: 2711 Founders Dr
contact.department: Poultry Science
contact.country: USA
contact.web_link: http://www.ncsu.edu/ashwell-lab/
contact.institute: North Carolina State University
contact.phone: 919-515-5537
organism: Gallus gallus
description: Due to array printer configuration, 320 wells were spoted but only 312 actually contained oligo material. The remaining 8 wells had only spoting solution. These wells were annotated as NA and were excluded from statistical analysis. Each well was spotted 3 times (side-by-side) in the same grid, and
  grids were replicated 4 times on each slide; so each gene was spotted a total of 12 times per array (3840). According to experimental design and to increase statistical power, the 3 side-by side spots were averaged so reducing the total number of data rows from 3840 to 1280.
manufacture_protocol: 1.A 384 well plate with the 70-mer oligos was synthetized by Operon Biothechnologies Inc.
  2. Oligos were resuspended in Universal Spotting Solution (Corning, Inc)
  3. Seal plates with Corning seals.
  4. Shake plate at mid speed on plate vortexer for 1 minute.
  5. Remove seal and place plate on appropriate place on VersArray ChipWriter array printer.
  6. Place UltraGaps Amino-Silane Coated Slides on appropriate place on array printer
  7. Run pin clean-up steps 5 times before start printing (using 4 pins)
  8. Start printing run
  9. Turn-off printer once run is completed
  10. Take plate from printer, seal it with Corning seal and store it at -20C
  11. Let slides dry on printer for 24h
  12. Crosslink slides on UV over set to 6000 x 100 µJ/cm2, store slides on secator