title: NCode miRNA microarray 474
status: Public on Jul 15 2010
contact.city: Jupiter
contact.first: Paul 
contact.middle: J.
contact.last: Kenny
contact.email: pjkenny@scripps.edu
contact.state: FL
contact.address: 130 Scripps Way
contact.department: Molecular Therapeutics
contact.country: USA
contact.institute: The Scripps Research Institute - Florida
contact.phone: 561 2282231
organism: Rattus norvegicus
manufacture_protocol: The microarray is contact-printed using microquill pins on Corning® Epoxide-Coated Glass Slides. The array specifications are: Total subarrays per slide: 3, Subarray layout: 8 blocks (4 rows × 2 columns), Block layout: 192 spots (12 rows × 16 columns), Block dimensions: 4 mm × 3 mm
  Average spot diameter: 120 μm, Spot center-to-center spacing: 265 μm. The array is printed with NCode™ miRNA probes, which are unmodified oligonucleotides (34–44 bases long) containing all known mature miRNAs in the miRBase Sequence Database, Release 9.0, for human, mouse, rat, D. melanogaster, C. elegans, and Zebrafish. Species-specific probes were designed using a proprietary algorithm that generates miRNA sequences with enhanced hybridization properties. The probes provide comparable sensitivity to wild-type sequences, while offering maximum specificity for discerning between closely related miRNAs and normalized melting temperatures for uniform hybridization. Probes have been validated for their ability to distinguish between wild-type and mismatched miRNA sequences with ≥1 base mismatch.