title: GAP_M_II
status: Public on Jan 01 2005
contact.city: Lynchburg
contact.first: Michael
contact.middle: S
contact.last: Price
contact.email: msprice2@liberty.edu
contact.state: VA
contact.address: 1971 University Blvd.
contact.department: Biology & Chemistry
contact.country: USA
contact.institute: Liberty University
contact.phone: 434-592-7277
organism: Aspergillus flavus
description: PCR products were generated using approximately 5 ng plasmid DNA isolated from unique cDNA clones. T3 and T7 primers were used to generate amplicons. PCR products were then purified by isopropanol precipation followed by a 70% ethanol wash. The presence of amplicons was verified by agarose gel electrophoresis.
  PCR products at approximately 250 ng/ul in 50% DMSO were spotted in triplicate onto Corning UltraGAPS slides (Corning, NY) using a Genetix Qarray Mini array printer (Genetix Limited, Hampshire, UK). Spots were allowed to air dry before being fixed following manufacturer’s recommendation.
  Keywords = aspergillus
  Keywords = aflatoxin