title: LC Mouse_Human_12.0_090114 miRNA Array (MRA-2001)
status: Public on Jan 30 2012
contact.city: Beijing
contact.first: Xiao
contact.last: Yang
contact.email: yangx@nic.bmi.ac.cn
contact.address: 20 Dongdajie
contact.country: China
contact.institute: Beijing Institute of Biotechnology
organism: Homo sapiens
  Mus musculus
description: Including:
  1) All mouse mature miRNAs listed in miRBase version 12.0
  2) Human mature miRNAs that are different from mouse miRNAs.
manufacture_protocol: Arrays are made based µParaflo microfluidic technology (Atactic Technologies). On the microfluidic chip, each detection probe consisted of a chemically modified nucleotide coding segment complementary to target microRNA (from miRBase, http://microrna.sanger.ac.uk/sequences/) or other RNA (control or customer defined sequences) and a spacer segment of polyethylene glycol to extend the coding segment away from the substrate. The detection probes were made by in situ synthesis using PGR (photogenerated reagent) chemistry. The hybridization melting temperatures were balanced by chemical modifications of the detection probes.