title: Cardiff Caenorhabditis elegans 20K oligo array (Operon oligonucleotide set version 1.1)
status: Public on Mar 23 2010
contact.city: Cardiff
contact.first: Peter
contact.last: Kille
contact.email: kille@cardiff.ac.uk
contact.address: Park Place
contact.department: Cardiff School of Biosciences
contact.country: United Kingdom
contact.web_link: http://kille.cf.ac.uk
contact.institute: Cardiff University
contact.phone: 02920874507
organism: Caenorhabditis elegans
description: Created from Operon C. elegans oligonucleotide set version 1.1.
  60-65mer oligonuleotides.
manufacture_protocol: 1. The microarrays were printed using Perkin Elmer SpotArray 72 printer (Perkin Elmer, MA, USA).
  2. Oligonucleotides  were resuspended in a water/DMSO 50/50 mix to a final concentration of 200 uM and Placed in 384 well plates with appropriate landmarks and oligonucleotides representing the Lucidea ScoreCard (GE Life Sciences).
  3. Each array was comprised of (12x4) 48 subarrays, each consisting of (20x22) 440 cDNA spots. In total, each array had 21,120 cDNA spots. This number of spots included the blanks, controls (lucidea scorecard), landmarks and the oligonucleotide reporters.
  4. Printing used 48 SMP3 Telechem split pins mounted on a Spotarray 72 (Perkin-Elmer).
  5. This printing regime yield spots of approximately 120 µm in diameter. Landmarks were introduced at the left-hand corner of each sub-array (and thus evenly spaced across the whole array) by the introduction of 3 replicates of the Lucida Scorecard (Amersham).
  6. Immobilization of the cDNA onto the CMT-GAPSTM coated glass slides was achieved by means of baking and UV crosslinking. The printed slides were initially immobilized by placing them in a UV StratalinkerTM 2400 bench top transilluminator (Stratagene Ltd) for five minutes, in order to initially immobilise the cDNA.
  7. The slides were then placed in a lightproof container and baked in an oven at 80oC for 2 hours. The slides were stored in a lightproof desiccator at room temperature, until required.