title: Human TKS4 isoform, TKS5 isoform, WIP, WASL, CR16, TTP custom gene expression assay (w/ Taq-Man probe)
status: Public on Aug 11 2025
contact.city: Kyiv
contact.first: Serhii
contact.last: Kropyvko
contact.email: s.v.kropyvko@edu.imbg.org.ua
contact.address: 150, Zabolotnogo str.
contact.department: Department of Functional Genomics
contact.country: Ukraine
contact.institute: Institute of Molecular Biology and Genetics NAS of Ukraine
organism: Homo sapiens
description: qPCR assay consists of 0.2μM of each specific primer, 0.1μM Taq-Man probe, 1.5mM MgCl2, 0.2mM dNTP, 2.5units DreamTaq DNA polymerase (Thermo Fisher Scientific, USA) and the corresponding buffer. Total volume - 25ul
manufacture_protocol: Primer design was performed with Oligo Primer Analysis Software v.7, the assay uses TBP expression as control.