title: UMGC-IRCNA 9k A
status: Public on Oct 18 2007
contact.city: Saint Herblain
contact.first: Wilfried
contact.last: Gouraud
contact.address: bd Jacques Monod
contact.department: Integrated Center of Oncology René Gauducheau
contact.country: France
contact.institute: ICO (Integrated Center of Oncology Nantes)
organism: Homo sapiens
manufacture_protocol: One channel DNA microarrays were constructed from EST cDNA clones (based on Homo sapiens).
  End-sequence-verified I.M.A.G.E. clones were purchased from RZPD German Resource Center for Genome Research (Berlin, Germany) or provided by the Human Genome Mapping Project Resource Centre (Hinxton, UK) and sequenced by MilleGen (Labege France).
  The cDNA clones were amplified in 96-well microtiter plates with universal primers.
  PCR products were spotted onto nylon using a robot-based high-speed contact spotting facility which ensures high precision positioning and constant droplet volumes of cDNA samples to provide an reproducible spot morphology and DNA content over the whole array.
  The process is performed under dust free, temperature-and humidity controlled conditions.
  The spotted cDNA is covalently bound by U.V. rays to the nylon surface.
  The feasibility, reproducibility and sensitivity of spotting procedures onto nylon membrane currently used in our laboratory to produce cDNA arrays have been previously described (Nguyen et al., Genomics. 1995;29:207-216; Bernard et al., Nucleic Acids Res. 1996;24:1435-1442; Bertucci et al. Hum Mol Genet. 1999;8:1715-1722; Magrangeas F et al. Blood. 2003;101:4998-5006.).