title: Tammar wallaby custom 13,440 spot cDNA array
status: Public on Nov 27 2014
contact.city: Bundoora
contact.first: Christy
contact.last: Vander Jagt
contact.email: christy.vanderjagt@depi.vic.gov.au
contact.state: Victoria
contact.address: 5 Ring Road, LaTrobe University
contact.department: Computational Biology
contact.country: Australia
contact.institute: Department of Environment and Primary Industries
contact.phone: +61 3 90327212
organism: Notamacropus eugenii
manufacture_protocol: 1. Approximately 100 pg cDNA per spot was spotted onto CMT-GAPS coated slides (Corning)
  2. Slide baked for 30 minutes at 80°C
  3. cDNA was then immobilised by treatment with UV light (650 x 100 Joules) in a Stratalinker (Stratagene)
  4. In preparation for hybridisation, slides were re-hydrated in water at 95°C for 5 minutes, followed by ethanol for 1 minute and dried by centrifugation (Sigma 4K15 centrifuge, Sigma) at 800 rpm for 3 minutes