title: National Yang-Ming University Homo sapiens 8K
status: Public on Dec 01 2006
contact.city: Taipei
contact.first: Ann-Ping
contact.last: Tsou
contact.email: aptsou@ym.edu.tw
contact.address: 155, Li-Nong St., Sec.2
contact.department: Inst. Biotechnology in Medicine
contact.country: Taiwan
contact.institute: National Yang-Ming University
contact.phone: 886-2-28267155
organism: Homo sapiens
description: Human hepatocellular carcinoma cells, namely, HuH7 were seeded. Samples were cultured in 0.1 μM sodium selenite-containing medium for 15 passages (SeLT) or in serum-free medium for 30 h (SF30) or in the regular complete medium containing 10 % fetal calf serum (HuH7) for 30 h. All the samples were collected at the time indicated above. The total RNA were reverse transcried and hybridized to Human 8K-1 (Egenomix Technology Corp., Taiwan). The hybridization time and temperature was 16 h and 62℃, respectively. SF30 sample was labeled with Cy3. SeLT and HuH7 samples were labeled with Cy5.
manufacture_protocol: Custom Array