title: Human 14K 70mer Oligo Array
status: Public on Jun 13 2015
contact.city: Calgary
contact.first: Don
contact.last: Morris
contact.email: don.morris@albertahealthservices.ca
contact.address: 1331, 29th Street, NW
contact.department: Oncology
contact.country: Canada
contact.institute: Tom Baker Cancer Centre
contact.phone: 403-521-3437
organism: Homo sapiens
manufacture_protocol: Human Genome Oligo Set(14K) from Qiagen, 70mer length was dissolved in 3XSSC in concentration of 40pmol.
  The 384 plates were stored at -80C untill printing day. The plates were transferred to -20C freezer in the morning for an hour and then spinned down for 20min at 1000rpm.
  The Oligo arrays were produced by Virtek Arrayer System SDDC-2 on Corning GAPS II Coated Slides.
  Oligos were arrayed on the slides. Each spot contains 600 pmol of 70-mer oligo.
  The slides were post-processed at 80C in an oven for 2 hours for fixation of the oligos.  Following this treatment, they were soaked in Succinane anhydride, N-methyl-pyrrolidinone and 0.2M NaBorate(pH 8.0) solution in order to block the oligos.
  The visualization of the printed oligos was performed by hybridizing the array with a Cy3-labeled random 9mer sequence at room temperature for the quality control of the array production.
  The spots size was 110um diameter.