title: MPIP24K
status: Public on Jan 31 2009
contact.city: München-Neuherberg
contact.first: Dietrich
contact.last: Trümbach
contact.email: dietrich.truembach@helmholtz-muenchen.de
contact.address: Ingolstädter Landstraße 1
contact.department: Institute for Developmental Genetics
contact.country: Germany
contact.institute: German Research Center for Environmental Health
organism: Mus musculus
manufacture_protocol: The cDNA microarray contains 24192 PCR products derived from different mouse cDNA libraries (sources: Research Genetics, RZPD Deutsches Ressourcenzentrum für Genomforschung, proprietary clones) representing 12387 different unigene clusters (unigene build #143). The spotting buffer contained 3 x SSC and 1.5 M betaine. The PCR products were spotted on GAPS II slides (Corning) using a Chipwriter Pro spotting robot (Biorad) and 48 SMP3 stealth pins (Telechem). After spotting, the arrays were heated to 80°C for 10 seconds and crosslinked using a UV Stratalinker 2400 at 200mJ (Stratagene). Slides were blocked 30 min at 65°C and prehybridized for 1 h at 42°C in mixture with 0.1 mg/ml BSA, 50% formamide, 5 x SSC and 0.1 x SDS. The arrays were rinsed with ultrapure water, then vigourously shaken for a few seconds in a 50 ml polypropylene tube containing isopropanol. The arrays were dried by centrifugation for 2 minutes at 400 g and hybridized within a few days.