title: Bovine oviduct and endometrium (BOE) cDNA array version 1
status: Public on Dec 31 2007
contact.city: Lindau
contact.first: Stefan
contact.middle: Michael
contact.last: Bauersachs
contact.email: stefan.bauersachs@uzh.ch
contact.state: Zurich
contact.address: Eschikon 27 EHB 23.1
contact.department: Department for Farm Animals
contact.country: Switzerland
contact.institute: University of Zurich
organism: Bos taurus
manufacture_protocol: Fifteen microliters of PCR reactions of amplified cDNA clones were transferred to 384 well microtiter plates (AbGene, Epsom, Surrey, UK) containing 15 µl of 2 x spotting buffer (40 mmol/l Tris-HCl pH 8, 2 M NaCl, 2 mmol/l EDTA, and bromphenol blue). PCR products were spotted onto amphoterous nylon membranes (Nytran plus, Schleicher & Schuell, Dassel, Germany) within an area of 2 x 5 cm. For spotting an Omnigrid Accent microarrayer (GeneMachines, San Carlos, CA) was used with solid pins (0.015 inches in diameter, SSP015; Telechem International; Sunnyvale, CA). Spotting was performed 6 times for each PCR product on the same position for sufficient and equal application. Twenty-eight arrays were produced simultaneously. The spotted DNA was denatured with 0.5 N NaOH for 20 min at room temperature. DNA was immobilized by baking for 30 min at 80 °C and UV crosslinking (120 mJ/cm2).