title: MPB Murine 32K Oligo printrun 4.1
status: Public on Jul 01 2011
contact.city: Winnipeg
contact.first: Rhiannon
contact.middle: LCH
contact.last: Huzarewich
contact.email: rhiannon_huzarewich@phac-aspc.gc.ca
contact.state: MB
contact.address: 1015 Arlington St.
contact.department: Molecular PathoBiology
contact.country: Canada
contact.institute: Public Health Agency of Canada
contact.phone: 2047847542
organism: Mus musculus
manufacture_protocol: Arrays were made using the mouse genome oligo set from Qiagen’s Array-Ready Oligo SetTM (AROS) version 3.0. Each set contained 31,769 arrayable 70mers representing 24,878 genes and 32,829 gene transcripts. The array design is based on the Ensembl (http://www.ensembl.org/) Mouse 14.30 Database and Mouse Genome Sequencing Project and contains alternative splicing variants using common, partial common, or individual transcript oligos. The set is provided in 84, 384-well plates containing 31,769 70mers each containing a 5’ C6 amino linker. Each well in the plate contained 600 pmol of the specified 70mer (including control elements) lyophilized and were resuspended in ArrayIt buffer (1x, Telechem) for a final concentration of 40 µM. After resuspension, the plates were resealed and shaken on an orbital shaker at 60 rpm overnight at room temp. The 70mers were spotted onto CMT-GAPS-coated glass slides (Corning, NY, Cat. 2549) using the Stealth micro-spotting pins (Telechem).