title: Custom Chironomus riparius Expression Array
status: Public on Jun 08 2012
contact.city: Amsterdam
contact.first: Wim
contact.last: de Leeuw
contact.email: w.c.deleeuw@uva.nl
contact.address: Science Park 904
contact.department: Swammerdam Institute for Life Sciences
contact.country: Netherlands
contact.institute: University of Amsterdam
contact.phone: +31(0)20-5257567
organism: Chironomus tentans
  Aedes aegypti
  Anopheles gambiae
  Culex quinquefasciatus
  Anopheles darlingi
  Anopheles funestus
  Chironomus riparius
description: To select the most reliable and biological relevant probes from the previously designed probe-library, it is necessary to develop a probe-selection microarray containing the probe-library and ample negative-control probes that do not to recognize any sequence in GenBank. The aCGH experiment subsequently allows the identification of probes that hybridize with the gDNA of the non-model organism. Assuming a limited chance on random homology, an aCGH experiment should allow considerable clean-up of the NGS data, eliminating NGS reads that originate from contamination or technological errors, and simultaneously allow selection of the relevant probes for the final expression microarray.
manufacture_protocol: NA