title: LC_MRA-2001_Mouse miRNA Array [miRMouse_17_SXH_110728]
status: Public on Feb 28 2019
contact.city: hunan
contact.first: Rongrong
contact.last: Cui
contact.email: rongrongcui@csu.edu.cn
contact.address: 139，renming road
contact.country: China
contact.institute: The second Xiangya hospital of central south university
organism: Mus musculus
description: Based on Sanger miRBase Release 17
manufacture_protocol: Custom sheep miRNA microarray targeting the mature miRNAs of Ovis aries. Arrays are made based µParaflo microfluidic technology (Atactic Technologies). On the µParaflo microfluidic chip, each detection probe consists of a chemically modified nucleotide coding segment complementary to target microRNA (from miRBase, http://miRBase.org) and sequences obtained by next-gen sequencing or other RNA (control or customer defined sequences) and a spacer segment of polyethylene glycol to extend the coding segment away from the substrate. The detection probes are made by in situ synthesis using PGR (photogenerated reagent) chemistry.