title: JCVI peanut oligonucleotide microarray (6,932 genes)
status: Public on Feb 19 2011
contact.city: Rockville
contact.first: Chun-Hua
contact.last: Wan
contact.email: cwan@jcvi.org
contact.state: MD
contact.address: 9704 Medical Center Dr
contact.department: Pathogen Functional Genomics Resources Center (PFGRC)
contact.country: USA
contact.institute: The J. Craig Venter Institute
contact.phone: 301-795-7707
organism: Arachis hypogaea
manufacture_protocol: At the final stage of the microarray design, the total oligos spotted on the microarray were 6,932, which represent 6,932 expressed peanut genes.  The oligos were synthesized by Sigma-Aldrich (Saint Louis, MO). The oligos were spotted on to Corning ultraGAPs glass slides with 3 replications at different location.  With dye-flip, the total was equivalent 6 replications to accommodate bioinformatics statistic analysis. There are 4 meta columns and 12 meta rows.  Each meta square consists of 24 sub-columns and 24 sub-rows.  This provides a capacity to accommodate 27,648 spots on each glass slide.  The distance between samples is 180 microns. The microarray printing was done on a 48 pen IAS-2 machine.  The oligo DNA was attached to the glass slide using UV Stratalinker.