title: McGill/Behr lab_M. tuberculosis_10K_v1.0
status: Public on Jul 22 2009
contact.city: Montreal
contact.first: Pilar
contact.last: Domenech
contact.email: pilar.domenech@mcgill.ca
contact.state: Quebec
contact.address: 1001 Décarie Boulevard
contact.department: IDIGH Program
contact.country: Canada
contact.institute: Research Institute of McGill University Health Centre
organism: Mycobacterium tuberculosis
description: 
  Contact information for the platform:
  Dr. Marcel A. Behr
  Division of Infectious Diseases and Medical Microbiology
  A5-156, Montreal General Hospital
  1650 Cedar Avenue
  Montreal, QC  H3G 1A4  Canada
  
manufacture_protocol: 1.Oligos are arrayed in Greiner 384-well flat-bottom plates. Each well contains 600 pmol of 70-mer oligo.
  2. Resuspend oligos in 3X SSC to 40 uM shake overnight at 4 degrees and rearray 5 µL into 384-well, Genetix polystyrene V-bottom plates (cat# X6004).
  3. Seal plates and store at -20 degrees until ready to print.
  4. Day of print, thaw plates and centrifuge plates at 2000 rpm for 2 minutes.
  5. Remove seals and place plate in the plate cassette.
  6. Arrays are spotted using a Virtek robot and the software that runs the system is VersArray ChipWriter Pro 2.1.1
  7. Make sure plates rest behind both holding clips in the cassettes. Push plates back into the cassettes as far as they will go, putting them in the proper position for the server arm.
  8. After the print run is completed, remove plates, seal and store at -20 degrees.
  9. For each subsequent preparation of these plates for a print run, verify if any evaporation has occured.  Add water to the wells if necessary.  The amount of water should be decreased by 0.25 µL per print run, as this is the amount drawn up by the pin capillary during each print.