title: CNIO Human Oncochip 1.0 , 1.2 and 2.0
status: Public on Jul 01 2008
contact.city: Madrid
contact.first: Mohit
contact.last: Aggarwal
contact.email: maggarwal@cnio.es
contact.address: Melchor F. Almagro 3
contact.country: Spain
contact.institute: CNIO
organism: Homo sapiens
manufacture_protocol: 1.- Individual I.M.A.G.E. Consortium [LLNL] clones were grown in 96-well plates at 37°C for 6 hours.
  2.- The bacterial cultures were used for PCR amplification.
  3.- PCR products were purified by ultrafiltration, verified by agarose gel electrophoresis and quantified by gel image densitometry
  4.- Probe concentrations were adjusted in 50% DMSO at about 100-150 ng/ul.
  5.- Probes were printed onto Gamma Amino Silane Coated glass slides (CMT-GAPS Corning) using the spotter Multigrid II (BioRobotics).
  6.- After printing, the slides were baked for 4 hours at 80ºC and stored desiccated in the dark, under vacuum and at room temperature.