title: Human ORESTES NoMatch 4.8k v1.0
status: Public on Apr 12 2009
contact.city: Sao Paulo
contact.first: Barbara
contact.middle: Pereira
contact.last: Mello
contact.email: bmello@lbhc.hcancer.org.br
contact.state: SP
contact.address: R. Prof. Antonio Prudente, 211
contact.department: Centro de Pesquisa
contact.country: Brazil
contact.institute: Hospital A. C. Camargo
contact.phone: 55-11-2189-5000-1134
organism: Homo sapiens
description: Glass slide array composed by ORESTES (open reading frame expressed sequence tags) from the HCGP (Human Cancer Genome Project) that did not align to known genes.
manufacture_protocol: Bacteria were grown in LB (Luria-Bertani) medium containing ampicillin (250mg/ml). Inserts were amplified by PCR using M13 reverse and forward primers and products were submitted to 1% agarose gel stained in ethidium bromide for quality control. cDNA clones with single amplicons were purified by filtration in Sephadex G50 (GE Healthcare), following the manufacturer’s directions, printed onto Corning glass slides (Corning) and fixed by UV crosslink.