title: Custom NimbleGen 12-plex Batrachochytrium dendrobatidis Oligo Expression Array
status: Public on Nov 01 2012
contact.city: Berkeley
contact.first: Thomas
contact.last: Poorten
contact.email: tom.poorten@gmail.com
contact.state: CA
contact.address: 54 Mulford Hall
contact.department: Environmental Science, Policy, and Management
contact.country: USA
contact.institute: UC Berkeley
organism: Batrachochytrium dendrobatidis
description: We designed a NimbleGen 12-plex microarray for Bd based on the publically available Bd genomes JAM81 (B. dendrobatidis Sequencing Project, Joint Genome Institute: http://genome.jgi-psf.org/Batde5/Batde5.download.ftp.html) and JEL423 (B. dendrobatidis Sequencing Project, Broad Institute of Harvard and MIT: http://www.broadinstitute.org/annotation/genome/batrachochytrium_dendrobatidis). The microarray contained 133,254 60-mer probes representing a possible 8,896 expressed transcripts (probe sets). Probe sets were defined as all probes that mapped to a single transcript, which in turn mapped to a single gene.
manufacture_protocol: See manufacturer's website