title: CapitalBioMiamiOtoArray Human Microarray
status: Public on Feb 17 2017
contact.city: Miami
contact.first: Denise
contact.last: Yan
contact.email: dyan@med.miami.edu
contact.state: FL
contact.address: 1601 NW 12th Ave
contact.department: Otolaryngology
contact.country: USA
contact.institute: University of Miami
contact.phone: 3052434923
organism: Homo sapiens
description: The microarray is composed of a fluidic manifold made of polycarbonate glued on the glass slide to introduce a dynamic hybridization for signal enhancement and acceleration of reaction kinetics. The tag probes contain a poly (dT) spacer and a 5' amino group, are diluted in 50% dimethyl sulfoxide (DMSO) to a final concentration of 15 micromole and are spotted with a SmartArrayer 136 spotter (CapitalBio) on the aldehyde-functionalized glass slides. Each tag probe is spotted in triplicate. The diameter of each spot is approximately 150 micrometers.
manufacture_protocol: http://www.capitalbiotech.com/en/products-content.html?id=65