title: [ChlpnV1a520166F] IHBI-C&T Chlamydia 2K v1.0_custom
status: Public on Sep 16 2010
contact.city: Brisbane
contact.first: Ashkan
contact.last: Amirshahi
contact.email: ashkan.amirshahi@qut.edu.au
contact.state: QLD
contact.address: 60 Musk ave, Kelvin Grove
contact.department: Infectious diseases
contact.country: Australia
contact.institute: Institute of Health and Biomedical Innovation (IHBI)
contact.phone: 617-31386254
organism: Chlamydia trachomatis
  Chlamydia pneumoniae CWL029
description: The custom Affymetrix microarray was designed from the genomes of the human pathogens Chlamydia pneumoniae CWL029 and Chlamydia trachomatis serovar D.
manufacture_protocol: The 2K microarray chip was designed with 11 sets of 15-mer oligonucleotide per defined open reading frame. Match and mismatched probes were generated using homologous sequences between the two defined species. Each gene-specific probe was designed to bind to the corresponding transcripts generated by the prokaryotic progenitors. Internal Affymetrix-specific controls for human, mouse, Escherichia coli and Bacillus sequences were included on the array for verification of hybridisation specificities. Proprietary Affymetrix hardware, software and spotting protocols were used in the construction of the custom array.