title: Mouse 13.4K long oligonucleotide array (Operon) 9.10.2002v2
status: Public on May 15 2004
contact.city: Malmö
contact.first: Harry
contact.last: Björkbacka
contact.email: harry.bjorkbacka@med.lu.se
contact.address: CRC 91:12, UMAS entrance 72
contact.department: Dept. of Clinical Sciences, Malmö
contact.country: Sweden
contact.web_link: http://www.med.lu.se/expcard
contact.institute: Lund University
organism: Mus musculus
description: The Operon version 1.1 oligonucleotide probe set (70-mers) was printed in the Massachusetts General Hospital microarray core facility using an OmniGrid 100 (GeneMachines) on CodeLink activated slides (Amersham).
  Oligos were printed in 150 mM sodiumphosphate pH 8.5 with 0.0005% N-lauroyl sarcosine.
  Oligos were coupled to the activated slides by allowing the aminolinker on the oligos to react over night in a humidity chamber.
  Unreacted groups on the slide surface were blocked with 150 mM glycine, 0.1 M tris, 0.1% N-lauroyl sarcosine, pH 9.
  Keywords = spotted, long oligonucleotide, Operon