title: JCVI PFGRC DeADMAn 12K v2 array designed primarily based on strain CDC1551
status: Public on Jan 25 2011
contact.city: Rockville
contact.first: PFGRC
contact.last: JCVI
contact.email: jli@jcvi.org
contact.state: MD
contact.address: 9704 Medical Center Drive
contact.department: PFGRC
contact.country: USA
contact.institute: J. Craig Venter Institute
contact.phone: 3017957840
organism: Mycobacterium tuberculosis CDC1551
description: This set includes 699 oligonucleotides, has 12288 spots distributed in 48 blocks. The DeADMAn DNA microarray is a companion reagent to be used in combination with pools of M. tuberculosis genetically defined transposon knockout mutants. Please see this page for the list of knockout mutants: http://www.beiresources.org/Catalog/ItemDetails/tabid/522/Default.aspx?BEINum=NR-18958&Template=otherProducts This microarray is used to provide a simple approach for identification of genes essential for the survival of a stress condition in in vivo model systems of M. tuberculosis infection. A pool of genetically defined transposon mutants (input pool) are used to infect an animal that is subsequently subjected to a stress condition. Mutants from the input pool and those recovered from the stressed animals (output pool) are identified by amplification of the unique transposon junction sequences of each mutant. The input and output pools are labeled with different fluorescent dyes and hybridized to the DeADMAn microarray. The competitive hybridization of the two pools to the DeADMAn microarray allows for the easy detection of mutants in the input pool and missing from the output pool. For additional information on the DeADMan microarray see: Lamichhane G, Tyagi S, Bishai WR. Designer arrays for defined mutant analysis to detect genes essential for survival of Mycobacterium tuberculosis in mouse lungs. Infect Immun. 2005 Apr;73(4):2533-40
manufacture_protocol: 2QDM, with arrayer AUSHON, machine cycles 0, pen id 0, pen cycles 0, operator 0, humidity control 0, humidity set point0.000