title: LaRiM Operon Human V3.0 printed oligonucleotide 36K array
status: Public on Jun 22 2012
contact.city: Rome
contact.first: Lucia
contact.last: Gabriele
contact.email: isslarim@gmail.com
contact.address: Viale Regina Elena, 299
contact.country: Italy
contact.institute: Istituto Superiore di Sanità
organism: Homo sapiens
manufacture_protocol: This array consists of 34,580 70mer oligonucleotide representing 24,650 genes and 37,123 gene transcripts (Operon Version 3.0 of the Human Genome Oligo Set). Oligonucleotides are consolidated into 384-well plates, lyophilized by speed-Vac, and resuspended in 3x SSC to a printing concentration of 30uM. Each oligonucleotide is spotted onto aminosilane glass slide SuperChip I (Cat No MPS696) provided by PerkinElmer Life Sciences Inc. using our robotic station GeneMachines OmniGrid 100 microarray printer (Genomic Solutions, Ann Arbor, Mich.) and Stealth Microspotting pins SMP3 (Telechem International, Sunnyvale, CA). The slides were:
  1. crosslinked in a UV Stratalinker 1800 (Stratagene) at 600mJ;
  2. washed in 1% SDS and 3X SSC on shaker for 1 min;
  3. washed in distilled water on shaker for 5 min (for two times);
  4. washed in distilled water to 100℃ for 20 sec;
  5. stored, ready to use,  in the dark under deep vacuum.