title: Center for Functional Analysis of Human Genome Human KUGI 24K (liver cancer)
status: Public on Oct 06 2005
contact.city: Daejeon
contact.first: Heun-Sik
contact.last: Lee
contact.email: hslee00@kribb.re.kr
contact.address: 52 Eoeun-dong, Yuseong-gu
contact.country: South Korea
contact.institute: Korea Reaearch Institute of Bioscience and Biotechnology
contact.phone: 82-42-879-8121
organism: Homo sapiens
manufacture_protocol: cDNA microarrays were fabricated in our core facility using a robotic arrayer (PixSYS 5500, Cartesian; Ann Arbor, MI). The 24K microarray contains 24,288 genes from KUGI unigene library (http://21cgenome.kribb.re.kr/html/2004_new/genome_php/cdna/cdna_list.htm). All the clones were verified by sequencing in our facility. The cDNA inserts were amplified by PCR, and after purification, spotted onto silylated glass slides (CSS-100; Telechem, Sunnyvale, CA). Each microarray slide contained control genes to normalize the effect of different fluorescent dyes.