title: Meyerowitz Lab Arabidopsis Operon Array Version 3
status: Public on Sep 07 2005
contact.city: Pasadena
contact.first: Frank
contact.last: Wellmer
contact.email: wellmer@caltech.edu
contact.state: CA
contact.address: 1200 E. California Blvd.
contact.department: Biology 156-29
contact.country: USA
contact.institute: California Institute of Technology
contact.phone: 626-395-8438
organism: Arabidopsis thaliana
description: This microarray is based on the Arabidopsis Genome Oligo Set Version 1.0 and on the Arabidopsis Genome Oligo Set Version 1.0 Upgrade (Operon, Alameda, CA). These sets consist of a total of 30,194 oligonucleotides that correspond to 26,753 annotated Arabidopsis genes.
manufacture_protocol: The oligonucleotides of the Arabidopsis Genome Oligo Set (Operon) were provided in 384-well plates. The dried DNA was resuspended in 15 ul of printing buffer (150 mM Na-phosphate pH 8.5) per well, resulting in an estimated DNA concentration of 40 uM. DNA was spotted onto poly-lysine coated glass slides with a MicroGrid II spotter (Biorobotics) using Microspot 10K quill pins (Biorobotics). After printing, the slides were processed and blocked following standard protocols.