title: NCI/ATC Mm-MEEBO ver. 07/27/09 (derived from GPL4200)
status: Public on Aug 01 2010
contact.city: Bethesda
contact.first: Snorri
contact.middle: S
contact.last: Thorgeirsson
contact.email: snorri_s_thorgeirsson@nih.gov
contact.state: MD
contact.address: 37 Convent Dr. # 4146
contact.department: Laboratory of Experimental Carcinogenesis
contact.country: USA
contact.web_link: http://neoplasia.nci.nih.gov/
contact.institute: National Cancer institute/NIH
contact.phone: (301) 496-5688 x204
organism: Mus musculus
manufacture_protocol: Microarrays are printed with GeneMachines robotic arrayer from Genomic Solutions (http://www.genomicsolutions.com) that is fitted with a printhead containing 48 SMP2.5 printing pins from Telechem International (http://www.arrayit.com). The mouse long oligonuleotide probe set contains 38,467 optimized 70mers (plus NCI Spotplate #4 controls) obtained from Invitrogen (http://www.invitrogen.com/). The long oligonucleotide probes were purchased in 384-well polypropylene plates (Genetix, 384 microarray) and dissolved print buffer of sodium phosphate (150mM) at ph 8.5 and 4% sodium sulphate at a concentration of 20-30 uM. The microarray spots are printed with 160 micron column x 155 microns row spacing, and one spot is printed per probe per slide. The microarrays were printed on Corning Expoxide slides and post-processed according to the manufacturer's protocol.