title: University of Malaga Pine Secondary Xylem 2.8K cDNA array version 1.0
status: Public on May 06 2012
contact.city: Malaga
contact.first: Rocio
contact.last: Bautista
contact.email: rociobm@scbi.uma.es
contact.address: Edificio de Bioinnovación, C/ Severo Ochoa, 34
contact.country: Spain
contact.institute: Plataforma Andaluza de Bioinformatica, Universidad de Malaga
organism: Pinus pinaster
manufacture_protocol: 1. Probes were PCR-amplified from a composite λ-ZAP cDNA library using T3 and T7 primers or M13 Forward and M13 Reverse
  2. PCR products were purified with 96-well multiscreen filter plates (Millipore Corp. Bedford, MA)
  3. Purified-cDNA probes in 50% DMSO were prepared to a concentration between 100 and 200 ng/µl in 384-well plates (Genetix)
  4. cDNA probe was printed in duplicate onto ULTRA gaps II coated slides (Corning, NY, USA) using a Qarray2 (Genetix Ltd, Queensway, UK) with a telechem printing head and 16 split pins (Biorobotics, Cambridge, UK) with a 4 x 4 configuration.
  5. After printing, the slides were dried at room temperature and the spotted cDNA were cross-linked to the slide surface by UV irradiation at 300 mJ/cm²