title: NCSU_Sulfolobus solfataricus P2_3Karray_version1
status: Public on Oct 29 2008
contact.city: Raleigh
contact.first: Charlotte
contact.last: Cooper
contact.email: crcooper@ncsu.edu
contact.state: NC
contact.address: 840 Main Campus Drive
contact.department: Chemical and Biomolecular Engineering
contact.country: USA
contact.institute: North Carolina State University
organism: Saccharolobus solfataricus
manufacture_protocol: A DNA microarray was designed and constructed based on at least 2976 protein-coding sequences in the S. solfataricus genome obtained from the TIGR CMR (http://cmr.tigr.org/tigr-scripts/CMR/CmrHomePage.cgi). OligoArray 2.1 (Rouillard, 2003) was used to generate one 60-mer (some slightly longer or shorter) oligonucleotide probe sequence for each open reading frame. The probes were synthesized (Integrated DNA Technologies, IA), re-suspended in 50% DMSO, and printed onto Ultragap microarray slides (Corning, NY) using a QArrayMini arrayer (Genetix, UK). Each probe was spotted four times onto each array to fortify statistical analysis.