title: HZAU_Tomato_12800_v.CGEP
status: Public on Dec 12 2012
contact.city: wuhan
contact.first: Fei
contact.last: Guo
contact.address: shizishan
contact.country: China
contact.institute: Huazhong Agricultural Univesity
organism: Solanum lycopersicum
manufacture_protocol: 1. TOM2 oligo array from tomato contain a total of 12800 elements or approximately 11860 unique elements.
  2. Sixty-nine oligonucleotides sequences from unigenes were synthesized by Operon (Operon biotechnologies, Hunstville, AL).
  3. Oligos were re-suspended in 12 uL of spotting buffer (3X SSC), and were spotted on Ultra Gaps Coated Slides (Corning, Lowell, MA) using a MicroGrid Pro arrayer (BioRobotics Inc., MA) with 32 MicroSpot2500 printing pins.
  4. Before printing, add 5 µL of 1X Printing Buffer to each well. This can be done the night before a print run is started.