title: CCDTM Hs_CCDTM17.5k
status: Public on Jun 23 2006
contact.city: Doha
contact.first: Francesco
contact.middle: Maria
contact.last: Marincola
contact.email: fmarincola@sidra.org
contact.address: Al Nasr Tower, AL Corniche Street, PO Box 26999
contact.country: Qatar
contact.institute: Sidra Medical and Research Center
contact.phone: 301-793-8210
organism: Homo sapiens
manufacture_protocol: Microarrays are printed with GeneMachines robotic arrayer from Genomic Solutions (http://www.genomicsolutions.com) that is fitted with a printhead containing 32 SMP2.5 printing pins from Telechem International (http://www.arrayit.com). The human cDNA probe set is based on RG_HsKG_031901 8k clone set and 9k clones from the RG_Hs_seq_ver_070700 40k clone set from Research Genetics (http://clones.invitrogen.com). The set includes 12,072 uniquely named genes, 875 duplicates of named genes and all remainder consisted of expression sequence tags (about 4053 EST). The cDNA probes were amplified by PCR and distributed into 384-well polypropylene plates (Genetix, 384 micro array) and dissolved in 3XSSC print buffer at a concentration of 50-100 ug/ml. The microarray spot densities are 280 microns (center-to-center), and one spot is printed per probe per slide. The microarrays are printed on Fisher Premium microscope slides (cat# 12-544-1) coated with poly-L-lysine and post-processed according to the procedures on the NCI website http://madb.nci.nih.gov/.