title: ENS SGDB Mus musculus NIA 15k
status: Public on Jun 30 2008
contact.city: Paris
contact.first: Stéphane
contact.last: LE CROM
contact.address: 46 rue d'Ulm
contact.department: Biology Institute - IBENS
contact.country: France
contact.institute: École normale supérieure
organism: Mus musculus
description: This cDNA clone set was prepared by researchers at the National Institute on Aging. The clone set has been distributed widely and is currently in use by a number of other microarray programmes.
  The set has the following characteristics:
  ~ 15,000 'unique' cDNA clones were rearrayed from 52,374 ESTs from pre- and periimplantation embryos, E12.5 female gonad/mesonephros, and newborn ovary.
  Up to 50% are derived from novel genes.
  ~ 1.5 kb average insert size.
  Clones were sequenced from 5' and 3' termini to obtain longer reads and verify sequence. Sequence information is available from their web site (NIA/NIH Mouse Genomics Home Page).
  PCR products were amplified by the National Sequencing Center.
manufacture_protocol: Slide spotted using a MicroGrid II (Genomic Solution)
  Spotting conditions: Temperature of 20¡C more or less 5¡C. Hygroscopy of 50% more or less 5%.
  Before each spotting:
  1.	Printing tips (Geneworx 10k) are tested on glass slides.
  2.	The spotting robot is put in operation and water tanks filled with distilled water.
  3.	Corning glass slides are deposited on plates.
  4.	The spotting program specific for each slide bacth is launched.