title: NCAUR/USDA-ARS Yeast 70-mer oligo array
status: Public on Dec 31 2010
contact.city: Peoria
contact.first: Z Lewis
contact.last: Liu
contact.email: zlewis.liu@ars.usda.gov
contact.state: IL
contact.address: 1815 N University
contact.country: USA
contact.institute: USDA-ARS
organism: Saccharomyces cerevisiae
manufacture_protocol: 1. 70-mer universal RNA controls were synthesized with AminoC6 modification at the 5' end
  2. Oligos are stored in Greiner 384-well flat-bottom plates. Each well contains 600 pmol of 70-mer oligo.
  3. Oligos stored at -80℃
  4. Allow oligos to thaw at room temperature
  5. Shake plate for 1 min
  6. Centrifuge plates at 2500 rpm for 1 min
  7. Remove seals and cover with plate lids
  8. Load the plates in a proper order onto a plate tower
  9. Array design with replicated genome in separated blocks
  10.Microarray spotting with a humidifier to keep constnet moisture in the chamber
  11. Array platform is properly leveled and examined for each direction
  12. Printing pins washed with sonication to avoid smaple contamination carry over