title: VUMC MACF human 19K oligo v33
status: Public on Jul 01 2006
contact.city: Amsterdam
contact.first: Daoud
contact.last: Sie
contact.email: d.sie@vumc.nl
contact.address: De Boelelaan 1117
contact.department: Pathology
contact.country: Netherlands
contact.institute: Vrije Universiteit Medical Center
contact.phone: +31 20 4442428
organism: Homo sapiens
description: - oligonucleotide sequences are available via www.ensembl.org after agreeing to Compugen's conditions
  - oligonucleotides were mapped by Ensembl to the NCBI35 version of the human genome
  - all plates were printed twice (duplicate spots)
manufacture_protocol: The Human Release 1.0 oligonucleotide library, containing 18861 60-mer oligonucleotides representing 17260 unique genes as designed by Compugen (San Jose, CA, USA) was obtained from Sigma-Genosys (Zwijndrecht, The Netherlands). The oligonucleotides were dissolved at 10 µM concentration in 150 mM sodium phosphate buffer pH 8.5. 384-well plates 1-39 containing 14485 unique oligonucleotides were spotted in duplo onto CodeLink™ slides (Amersham Biosciences) using an OmniGrid® 100 microarrayer (Genomic Solutions, Ann Arbor, MI, USA) equipped with SMP3 pins (TeleChem International, Sunnyvale, CA, USA). After printing slides were processed and blocked prior to use according to the manufacturer’s protocol.