title: Protein Microarray (spotted Outer Membrane Vesicles(OMVs) of E. coli)
status: Public on Apr 08 2025
contact.city: Siena
contact.first: Viola
contact.last: Viviani
contact.email: viola.x.viviani@gsk.com
contact.address: Via Fiorentina, 1
contact.country: Italy
contact.institute: GSK
organism: Escherichia coli
manufacture_protocol: Recombinant E. coli GMMA a  were prepared at 1 mg/ml or 0,5mg/ml in 20% glycerol and then serially diluted in 20% glycerol (step-2 dilutions).  Samples were transferred into 384-well plates. Two droplets of 0.1 nL protein solution was deposited per spot on nitrocellulose-coated slides (FAST slides, Maine Manufacturing) using the non-contact printer Marathon Argus Spotter (Arrayjet). Printing was performed in a cabinet with controlled humidity and temperature (50-55% and 18°C, respectively). Each protein was printed in replicates on each slide. Printed protein microarrays were kept at room temperature for 5 hours, dried under a stream of nitrogen and store at 4°C. Only the highest concentration of the vesicles was included in the final dataset.