title: University of Queensland Human SSH19kv4
status: Public on Sep 24 2009
contact.city: St Lucia
contact.first: Rosemarie
contact.middle: Lotte
contact.last: Kryger
contact.email: rosemarie.kryger@uqconnect.edu.au
contact.state: Qld
contact.address: Sir Fred Schonell Drive
contact.department: Biochemistry
contact.country: Australia
contact.institute: University of Queensland
contact.phone: 61 7 33741690
organism: Homo sapiens
manufacture_protocol: 1. DNA are arrayed in Greiner 384-well v-bottom (source) plates. Each well contains 0.1ug/ul to 0.25ug/ul
  2. Source plates are dried in SpeedVac
  3. DNA is resuspended in 6 ul of printing buffer.
  4. Source plates are Incubated at room temperature for 30 minutes to aid resuspension of DNA.
  5. Source plates are sealed with Corning seals and stored at 4°C
  6. Before printing, source plates are centrifuged at 1500 rpm for 5 minutes.
  7. Remove seals and cover with plate lids and place source plates for printing in the robotic fixture.
  8. After the print run is completed, plates are sealed and stored at 4°C.