title: Drosophila Chromosome 2L microarray (based on Dmel release 3.0)
status: Public on Oct 27 2007
contact.city: Basel
contact.first: Oliver
contact.last: Bell
contact.email: oliver.bell@fmi.ch
contact.address: Maulbeerstr. 66
contact.department: Epigenetics
contact.country: Switzerland
contact.institute: Friedrich Miescher Institute
contact.phone: +41 (61) 697 8270
organism: Drosophila melanogaster
description: The genomic array used in this study, dros_chrom_array_MIT_120803, was designed in the laboratory of Stephen P. Bell at the Massachusetts Institute of Technology. The array contains 13440 features spotted on glass Gaps II slides (Corning) in 32 meta grids. There are 11816 1.5 kb pcr products that tile the non-repetitive sequences of chromosome 2L. In addition there are 287 1.5 kb features from chromosome 4, 90 features from chromosome 3L, 177 features from chromosome 3R and finally 48 features corresponding to telomeric sequences. The features were all amplified from BACs and visually verified. The oligos for amplification were designed from the final release candidate for release 3.0 of the Drosophila genome using custom perl scripts and primer3. The primers were synthesized by Operon.
  The file, dros_chrom_array_MIT_120803.csv, describes and provides coordinates for all the features on the array.
manufacture_protocol: For details see http://chromosome2l.mit.edu/array/