title: miRNA expression in Human Uterine Leiomyomata
status: Public on Apr 15 2007
contact.city: Newark
contact.first: Tongsheng
contact.last: Wang
contact.email: wang@phri.org
contact.state: NJ
contact.address: 225 Warren Street
contact.department: Center For Applied Genomics
contact.country: USA
contact.institute: Public Health Research Institute
organism: Homo sapiens
manufacture_protocol: DNA oligonucleotide probes from the mirVanaTM miRNA probe set (Ambion, Austin, TX) were used to print custom microarrays.  The miRNA microarrays consist of 206 oligos representing 206 well-characterized miRNAs, each oligo was spotted in triplicate on the chip. Probes were resuspended in 3X SSC at 50uM concentration and spotted on Nexterion Slide E (SCHOTT North America Inc. Louisville, KY) at 50% humidity using an OmniGrid100 arrayer (Genomic Solutions). The printed slides were blocked in a solution containing 100mM ethanolamine, 1M Tris (pH 9.0), and 0.1% SDS for 15 min at 50 ºC, and then thoroughly rinsed with water and spun dry.