title: CEA Yeast 7K Intergenic Microarray
status: Public on Sep 27 2005
contact.city: Gif Sur Yvette
contact.first: Michel
contact.last: Werner
contact.email: mwerner@cea.fr
contact.address: RN 306
contact.department: Biologie Joliot-Curie
contact.country: France
contact.institute: Commissariat à L'Energie Atomique-Saclay
contact.phone: +33 1 69 08 93 42
organism: Saccharomyces cerevisiae
description: -Platform: amino-silane coated glass slides (GAPS II, Corning)
  
  -S. cerevisiae intergenic regions amplified from S288C genomic DNA (ResGen) using the intergenic region primer oligonucleotides (ResGen) (Harismendy et al. EMBO J. 22(18): 4738-4747, 2003). The primers allow the amplification of the sequence located on either side of elements such as open reading frames, tRNAs, small nuclear RNAs, Ty elements, solo δ, etc. A complete description of the primers can be obtained on the web site (ftp://ftp.resgen.com/pub/genepairs/yeast_intergenic).
  
  -The PCR products were purified and spotted using an automated arrayer (MicroGrid II, BioRobotics)
manufacture_protocol: See Description