title: MEEBO Mus musculus Oligo 70mer Array V1.1
status: Public on Sep 11 2008
contact.city: San Francisco
contact.first: Sajeev
contact.last: Batra
contact.state: CA
contact.address: Box 2240, 600 16th Street S474
contact.country: USA
contact.institute: UCSF
organism: Mus musculus
manufacture_protocol: Oligos are arrayed in Genetix x7020 384-well plates.  Each well contains 200pmol of 70-mer oligo.  During the first round of resuspension, oligos were resuspended in 3XSSC to a final concentration of 30 micromolar.  After each print, oligos are dried in a vacuum centrifuge.  During subsequent prints, oligos are resuspended in water to a final concentration of 30 micromolar taking into consideration the amount of oligo printed in the previous printrun.  Oligos are printed using 48 Parallel Synthesis silicon 50 micrometer printing tips on a DeRisi-style custom built microarrayer.  A sector width of 28 and a spacing of 155 microns is employed.