title: CGC Legionella whole-genome microarray v1.0
status: Public on Sep 12 2008
contact.city: New York
contact.first: Sergey
contact.last: Kalachikov
contact.email: sk363@columbia.edu
contact.state: NY
contact.address: NWC Bld, 550 West 120th Street Rm 1002
contact.department: Chemical Engineering
contact.country: USA
contact.institute: Columbia University
contact.phone: (212)851-9193
organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
manufacture_protocol: 70-mer oligonucleotides specific for each of the unique genes in the Legionella pneumophila subsp. pneumophila str. Philadelphia 1
  genome (2976 total of the unique probes) were dissolved in 50% DMSO at 30 micromolar concentration and spotted in duplcate on Corning UltraGAPS microarray coated slides (Corning) using SpotArray 72 Microarray printing system (Perkin Elmer). After the manufacturing the arrays were stored dessicated in vacuum at ambient temperature until further use. Piror to use, the oligonucleotides were UV-crosslinked to the slide surface.