title: CombiMatrix 90K Petunia inflata array
status: Public on Feb 29 2012
contact.city: Verona
contact.first: Sara
contact.last: Zenoni
contact.email: sara.zenoni@univr.it
contact.address: Le Grazie
contact.department: Biotecnology
contact.country: Italy
contact.institute: University of Verona
contact.phone: 045 8027941
organism: Petunia integrifolia subsp. inflata
description: 29,163  specific oligos (35-40mer) were designed on Petunia inflata tentative consensus (TC) sequences (JI359155 - JI387099), and by using the program design OligoArray 2.1 (Rouillard et al. 2003). As negative controls, nine bacterial oligonucleotides sequences provided by CombiMatrix were utilized. The 90K TomatArrayTM 1.0 was designed with four replicates of each probe randomly distributed across the array to allow measurement of the variability within the array. All the procedures provided were performed as indicated by CombiMatrix protocols available at the CombiMatrix website (www.combimatrix.com).
manufacture_protocol: Probes are directly synthesized on a 90k Combimatrix array by an in situ oligo synthesis system developed by CombiMatrix Corporation (Mukilteo, USA) using the CombiMatrix CustomArray synthesizer. Standard synthesis protocol provided by CombiMatrix was used.