title: Operon Homo sapiens 21K (Hs Operon Vw)
status: Public on Apr 19 2007
contact.city: San Francisco
contact.first: Yuanyuan
contact.last: Xiao
contact.email: yuanyuan.xiao@ucsf.edu
contact.state: CA
contact.address: 185 Berry Street, Lobby4, Suite 5700
contact.department: Epidemiology and Biostatistics
contact.country: USA
contact.institute: UCSF
organism: Homo sapiens
manufacture_protocol: Long oligonucleotides were designed and synthesized by Operon Biotechnologies (Operon Biotechnologies, Huntsville, AL). The Human Genome Oligo Set Version 2 includes 21,329 oligonucleotides, mostly 69-mers that were based upon UniGene build 147. Oligonucleotides were dissolved in 3XSSC at a concentration of 40um in preparation for spotting on aminosilane-coated slides (Schott Nexterion, Elmsford, NY). A custom-built microarrayer equipped with Majer Precision MicroQuill 2000 pins was used to print each batch of 255 slides. A detailed description of the microarrayer (MGuide) is available at the Brown lab's website (Stanford Univeristy). After printing, the slides were stored at room temperature in desiccators.