title: Human 36mer genome tiling array HS_14289
status: Public on Oct 29 2004
contact.city: New Haven
contact.first: Paul
contact.last: Bertone
contact.email: paul.bertone@yale.edu
contact.state: CT
contact.country: USA
contact.institute: Yale University
contact.phone: (203) 432-5405
organism: Homo sapiens
description: Maskless photolithographic synthesis oligonucleotide array on glass
  This series of microarrays is designed to represent sense and antisense strands of the nonrepetitive DNA of the human genome with 36mer oligonucleotide probes, positioned every 46nt on average. The probes were selected from the November 14, 2002 human genome sequence assembly (NCBI Build 31/hg13).
  Microarrays were constructed with Expedite DNA synthesizers (Applied Biosystems, Foster City, CA) in conjunction with Maskless Array Synthesis (MAS) units (NimbleGen Systems, Madison, WI).
  Arrays containing approximately 390,000 oligonucleotide features in a 14 × 17.4 mm square area were fabricated with photolabile phosophoramidites (NPPOC-dAdenosine (N6-tac) -Cyanoethylphosphoramidite, NPPOC-dCytidine (N4-Isobutyryl) -Cyanoethylphosphoramidite NPPOC-dGuanosine (N2-ipac) -Cyanoethylphosphoramidite, NPPOC-dThymidine-Cyanoethylphosphoramidite) obtained from Proligo (Boulder, CO). Following MAS synthesis, base-protecting groups were removed in a solution of ethylenediamine:ethanol (1:1 v/v) (Aldrich, St. Louis, MO) for 2 hours. The arrays were rinsed with water, dried and stored desiccated until use.
  Keywords = genomic tiling, maskless photolithography