title: Human 58K oligonucleotide chip
status: Public on Aug 19 2005
contact.city: Odense
contact.first: Mads
contact.last: Thomassen
contact.email: mads.thomassen@ouh.fyns-amt.dk
contact.address: Soender Boulevard 9
contact.department: Clinical Genetics (KKA)
contact.country: Denmark
contact.institute: Odense University Hospital
contact.phone: +4565412839
organism: Homo sapiens
description: 29,134 oligonuclaotides recognising 28,800 genes or EST designed by Compugene and produced by Sigma
manufacture_protocol: A human oligonucleotide library composed of 29,134 oligonucleotide targets (60mers) recognising 28,830 genes was purchased from Sigma-Genosys (The Woodlands, TX) and designed by Compugen (Compugen Ltd., Jamesburg, NJ) with a 5´ amino linker modification. The oligonucleotides have been designed to include known genes, expressed sequence tags, the majority of known alternative splice variants (http://www.labonweb.com/oligo), and spike targets.
  According to the CodeLinkTM protocol, oligonucleotide targets were solubilized in 150 mM sodium phosphate buffer (pH 8.5) and divided into a single aliquot and applied to 76 384-well source plates using an Eppendorf epMotion 5070 robot (Radiometer, DK) at a final concentration of 20pmol/µl. Oligonucleotide targets were arrayed in duplicate by a spotting robot (Virtek ChipWriter Pro (ESI)) onto 50 slides in a controlled environment with a humidity of 38 % inside the spotting compartment. Duplicate spots were 9 mm apart. 24 SMP 2.5 stealth pins (TeleChem International Inc., Sunnyvale, USA) were used for printing and each spot were about 100 µm in diameter and spot centres were 124 µm apart. After printing, slides were incubated overnight at 70% humidity and blocked as recommended by the manufacturer. Dried microarray slides were stored in a desiccated environment at room temperature.