title: Affymetrix GeneChip Custom-Made Muscle Chip
status: Public on Feb 10 2003
contact.city: Binghamton
contact.first: Eric
contact.middle: P
contact.last: Hoffman
contact.email: ericphoffman@gmail.com
contact.state: NY
contact.address: PO Box 6000
contact.department: Pharmaceutical Sciences
contact.country: USA
contact.institute: Binghamton University - SUNY
organism: Homo sapiens
description: MuscleChip containing 4,601 muscle probe sets representing 1150 known genes expressed in muscle, and 2,075 EST clusters from a non-normalized subtracted muscle sequencing project (28,074 EST sequences).  This included 460 novel EST clusters, showing no match to previously characterized genes in any database.   Each probe set was designed to contain 20-32 25mer oligonucleotides (10-16 paired perfect match and mismatch probe pairs per gene), with each probe evaluated for hybridization kinetics (Tm), and similarity to other sequences.   Approximately 120,000 oligonucleotides were synthesized by photolithography and light-activated chemistry per microarray.