title: CSIR Fungi 20090901 96 v1.0
status: Public on Mar 01 2010
contact.city: Pretoria
contact.first: Eugenia
contact.last: Barros
contact.email: slezar@csir.co.za
contact.state: Gauteng
contact.address: Meiring Naude Rd
contact.department: Building 20
contact.country: South Africa
contact.institute: CSIR
contact.phone: 0027-12-8413221
organism: Aspergillus
  Aspergillus amstelodami
  Aspergillus clavatus
  Aspergillus niger
  Penicillium
  Fusarium
  Fusarium oxysporum
  Fusarium sporotrichioides
  Fusarium acuminatum
  Fusarium graminearum
  Alternaria alternata
  Penicillium expansum
  Talaromyces islandicus
  Fusarium avenaceum
  Aspergillus carbonarius
  Fusarium subglutinans
  Aspergillus versicolor
  Fusarium cerealis
  Fusarium equiseti
  Penicillium fellutanum
  Penicillium corylophilum
  Fusarium verticillioides
description: Forty food-borne fungi
manufacture_protocol: 1. Oligonucleotide probes  ranging in size from 16 - 23 bases were transferred to Genetix X7022 384-well plates for arraying.
  2. Each well contained a final concentration of 20 µM oligonucleotide probe in 50% DMSO spotting solution (v/v).
  3. Between printing runs the plates were stored at -20°C.
  4. Prior to printing the plates were incubated at room temperature for 30 min to aid temperature equilibration.
  5. Plates were centrifuged at 2000 x g for 5 minutes prior to printing.
  6. Printing was performed on the Molecular Dynamics Generation III spotter.
  7. Seals were removed and plates placed in the spotter immediately prior to initiating the printing process to prevent unnecessary evaporation.
  8. A relative humidity of 50% was maintained in the spotter cabinet during printing runs.
  9. Following completion of the printing run the plates were sealed with a new Abgene adhesive plate seal and stored at -20°C.