title: LC Sciences Pig miRNA array (miRPig_18_SCA_120119)
status: Public on May 05 2015
contact.city: Guangzhou
contact.first: Ruisong
contact.last: Ye
contact.email: 51684804@qq.com
contact.state: Guangdong
contact.address: 483 Wushan Tianhe
contact.country: China
contact.institute: South China Agricultural University
contact.phone: 8615989088540
organism: Sus scrofa
description: Pig miRNAs microarray includes all porcine miRNAs deposited in miRBase 18.0 and designed sequences as miRNA microarray probes.On  the  microfluidic chip,  each  detection  probe consisted  of  a  chemically  modified  nucleotide  coding  segment complementary to target  microRNA (from  miRBase, http://microrna.sanger.ac.uk/sequences/)or other RNA  (control or customer definedsequences) and a spacer segment of polyethylene glycol to extend the coding segment away from the substrate.The detection probes  were made by in situ synthesis using PGR (photogenerated reagent)chemistry
  In detail, miRBase 18.0 collected a total of 257 porcine miRNAs (255 miRNAs according to its sequence). We added 100 probes (probes labelled with prefix 'Y-') according to our previous reports.
manufacture_protocol: see manufacturer's website