title: Custom Febit Aspergillus nidulans biA1 veA1 biochip
status: Public on Jul 01 2014
contact.city: Sevilla
contact.first: Gabriel
contact.last: Gutierrez
contact.address: PO BOX 1095
contact.department: Genetics
contact.country: Spain
contact.institute: University of Seville
organism: Aspergillus nidulans
manufacture_protocol: The Geniom biochips used for this study were synthesized with the Geniom One instrument applying febit's standard shortmer kit for oligonucleotide synthesis. The Biochip was manufactured exactly to precise specifications and achieved the standard quality criteria. The light-activated in-situ oligonucleotide synthesis using a digital micromirror device was performed within the Geniom One instrument on an activated three-dimensional reaction carrier consisting of a glass-silicon-glass sandwich (Biochip). Using standard DNA synthesis reagents and 3'-phosphoramidites carrying a 5'-photolabile protective group, oligonucleotides were synthesized in parallel in eight individually accessible microchannels (referred to as arrays) of one biochip. Prior to synthesis, the glass surface was activated by coating with a spacer to facilitate probe-target interaction and to avoid probe-probe interference.