title: INRA-BDR Bovine D14 Embryo 1K
status: Public on Dec 20 2007
contact.city: Jouy en Josas
contact.first: Isabelle
contact.last: Hue
contact.email: isabelle.hue@jouy.inra.fr
contact.address: Domaine de Vilvert
contact.department: PHASE
contact.country: France
contact.institute: INRA
contact.phone: 33134652567
organism: Bos taurus
description: (8cmx12cm, Amersham Biosciences) 5x5 pattern
manufacture_protocol: cDNA inserts from the bcai arrayed library (bcai library indexed in TIGR as #FJB and in NCBI as "15979") were amplified by PCR using the flanking primers used to construct this SMART-amplified library (PCRp1 primers from the Cap Finder cDNA kit, Clontech). After a short run on a 2.5% agarose gel, the probes were selected (size >= 500pb), spotted and fixed (UV light, 1 min, 1200J, twice) onto nylon N+ membranes (8cmx12cm, Amersham Biosciences) with a 5x5 pattern (BioRobotics). The whole array represents 1920 probes but the biological core of the array (1K array) represents only 987 informative probes (1097 sequences submitted to the EBI deprived of the 110 mitochondrial sequences).