title: Delta gene expression assay with Fluidigm 96 dynamic array
status: Public on Mar 19 2022
contact.city: Paris
contact.first: Olivia
contact.last: Bonduelle
contact.email: olivia.bonduelle@sorbonne-universite.fr
contact.address: 91 bd de l'Hopital
contact.country: France
contact.institute: INSERM U1135
organism: Mus musculus
description: Fluidigm 96 dynamic array. PCR assay
  As a result of the lognormal distribution described by Bengtsson et al. (2005) and others, it is useful to view single-cell data as expression level above detection limit on a log scale. For qPCR data, it is convenient and appropriate to do this in Log2 by defining the term Log2Ex: Log2Ex = LoD Ct ? Ct [Gene]. ?If the value is negative, Log2Ex = 0. ?For more information on this value, see K.J. Livak et al., Methods (2012),http://dx.doi.org/10.1016/j.ymeth.2012.10.004.
manufacture_protocol: see manufacturer website: Real-Time PCR Analysis protocol user guide and "Two-step single-cell gene expression using EvaGreen Supermix on the BioMark and BioMark HD systems, protocol C"