title: VRI/Bact Salmonella Typhimurium LT2 5.6K v1.0
status: Public on Oct 24 2007
contact.city: Brno
contact.first: Magdalena
contact.last: Crhanova
contact.email: crhanova@vri.cz
contact.address: Hudcova 70
contact.department: Bacteriology
contact.country: Czech Republic
contact.institute: Veterinary research institute
organism: Salmonella enterica subsp. enterica
manufacture_protocol: Salmonella Genome Array-Ready Oligo SetTM Version 1.0 (Operon)
  1. Oligos are provided in 384-well flat-bottom plates. Each well contains 600 pmol of 70-mer oligo.
  2. Resuspend oligos in water to 40 µM and add 15 µL of 2x Spotting Solution Plus (Telechem) to final concentration 20 µM.
  3. Rearray 15 µl from each well into new 384-well polystyrene plate. Use one of the plates for spotting and the second as stock.
  4. Seal plates with Corning seals and store at -20°C.
  5. Before printing defrost in laboratory temperature.
  6. Centrifuge plates at 2000 rpm for 3 minutes.
  7. Remove seals immediately before printing.
  8. Print in spotter (Cartesian Technologies PixSysTM PA Serier, Version 4.5) using software CloneTrackerTM Version 1.4.
  9. After the print run is completed, seal plates with Corning seals and store at -20°C.
  10. Incubate the printed slides at 75% relative humidity overnight for maximum DNA immobilization.
  11. Store immobilized microarray slides in the dark and dry.