title: Maize oligo array version 1.4 slide set (slides A and B)
status: Public on Nov 30 2010
contact.city: St. Louis
contact.first: Nicole
contact.middle: Christina
contact.last: Riddle
contact.state: MO
contact.address: 1 Brookings Dr.; CB1137
contact.department: Biology
contact.country: USA
contact.institute: Washington University
organism: Zea mays
manufacture_protocol: REHYDRATION AND PRINTING OF THE OLIGONUCLEOTIDE MICROARRAYS Add 15 uL of 3XSSC to each well of the 300 pM oligonucleotide set to make a 20 μM oligonucleotide solution. Transfer onto an orbital shaker for 1 h at RT at 100 rpm. The oligonucleotides are now ready for printing. The Arabidopsis oligonucleotide microarrays are printed on aminosilane-coated “Superamine” slides (Telechem) or “Powermatrix” slides (Full Moon Biosystems), using Telechem Type SMP3 printing pins. Printing conditions: Single array element printed per oligonucleotide, 48 pins in the print head, 31% relative humidity, redipping after every 50 slides. The print head Z- velocity is set to a maximum of 10 mm/sec. Bake slides for 2h at 80oC. Store dry and in darkness at RT.
  IMMOBILIZATION OF THE OLIGONUCLEOTIDE ARRAY ELEMENTS Rehydrate the microarray slide over 50oC water for 10 seconds; snap-dry on a 65oC heating block for 10 seconds. Repeat this step 4 times. Crosslink the oligonucleotides to the slide surface by exposing the DNA-side of the microarray to UV irradiation (65mJ). Wash the slides in 1% SDS dissolved in sterile ddH2O for 5 min at RT. Rinse the slides by plunging slide into sterile ddH2O. Rinse in 100% ETOH for 30 seconds to 5 min, with gentle shaking. Spin dry the slides in a slide centrifuge for 3 min. Store the slides in a light-proof box under cool dry conditions.