title: Agilent-033170 Manila clam 8x15k oligo microarray
status: Public on Oct 01 2013
contact.city: Legnaro
contact.first: Massimo
contact.last: Milan
contact.email: massimo.milan@unipd.it
contact.state: PD
contact.address: Viale dell'Università 16
contact.department: Dept. of Department of Public Health, Comparative Pathology,and Veterinary Hygiene
contact.country: Italy
contact.institute: University of Padova
contact.phone: +39 0498272941
organism: Ruditapes philippinarum
description: GE 8x15k oligo microarray that covers 11,906 transcripts of the Manila clam (Ruditapes philippinarum). Oligonucleotides, 60-mer plus a “linker”, are synthesized directly on glass slide using the Agilent’s SurePrint Tecnology. A total of 11,197 probes were already employed on a previous version of the microarray (GPL10900) and maintained the same Probe ID (contig ID plus suffix _1, _2, _AS or _S). To them, one  probe (contig ID without any suffix) for  transcripts with known orientation was added. In addition, 2 probes with different orientation (sense and antisense) were designed for transcripts with unknown orientation. Manila clam 18X5 microarray represent a total of 14,156 probes representing 11,906 transcripts.Both independent probes designed for the same transcript are named with an univocal ID; the sense probes are distinguished by suffix_S while the anti-sense probes are distinguished by suffix _AS. As for probes employed on GPL10900, sequence and description of each gene (contig) are available in the Ruphidatabase (Milan et. al 2011),  where contig ID corresponds to probe ID  on the array.
manufacture_protocol: See SurePrint Technology at manufacturer's web site http://agilent.com/