title: GreenGene/Operon/Galbraith lab Rice 60K Oligo Array
status: Public on Dec 07 2010
contact.city: Suwon
contact.first: Myoung Ryoul
contact.last: Park
contact.address: 126 Suin-ro
contact.country: South Korea
contact.institute: National Institute of Crop Science
organism: Oryza sativa
manufacture_protocol: The 60K Microarray was designed to represent all the genes in rice. In total, 60,727 oligomers were designed from gene-specific regions of both japonica and indica subsp. These include 58,417 from known and predicted genes and 66 randomized DNA. Among these, 2,310 genes were also designed as antisense oligomers. Oligomer sequences were extracted by Qiagen-Operon (Cologne, Germany) based on rice genome information from Beijing Genomics Institute (Yu et al., 2002). Oligomers were synthesized and purified by Qiagen-Operon and spotted on SuperAmine slide using facilities of the Dr. David Galbraith lab at the University of Arizona. A set of Rice 60 K Microarray is composed of 2 slides and has a total of 64,896 spot addresses. Each slide is formatted with 48 (12 × 4) blocks with addresses composed of 676 (26 × 26) spots. Blank spots (4,099) were also included for easy alignment of scanning format. Each oligomer, 70 nucleotides long and average melting temperature of 78℃, was printed in each spot address with a diameter of 100 ㎛.