title: CCRI/Munson Haemophilus influenzae (NTHI) 11k spotted array v1.00
status: Public on Sep 09 2006
contact.city: Columbus
contact.first: William
contact.middle: Clarence
contact.last: Ray
contact.email: ray.29@osu.edu
contact.state: OH
contact.address: 700 W. Children's Drive. Rm W531
contact.department: Center for Microbial Pathogenesis
contact.country: USA
contact.institute: Columbus Children's Research Institute
organism: Haemophilus influenzae Rd KW20
  Haemophilus influenzae 86-028NP
manufacture_protocol: The commercial oligo set for H. influenzae Rd KW20 was purchased from Operon.  548 additional custom 70-mers were designed and synthesized for ORFs unique to/with insufficient sequence similarity to H. influenzae strain 86-028NP.  Oligos were resuspended in 2.5xSSC to a final concentration of 25uM and arrayed in quadrupliate at 55% RH, using a Genetix QArray2 arrayer on Corning UltraGAPS slides.  Printed Arrays were re-hydrated and snap dried on a hot plate at 100C for 10s.  DNA was fixed to the surface using a UV cross-linker at 650mJ/cm^2