title: UCSF/Sil lab-Histoplasma capsulatum-G217Borfs2
status: Public on Nov 22 2011
contact.city: San Francisco
contact.first: Mark
contact.last: Voorhies
contact.email: mark.voorhies@ucsf.edu
contact.state: CA
contact.address: 513 Parnassus, S472
contact.department: Microbiology and Immunology
contact.country: USA
contact.institute: University of California
organism: Histoplasma capsulatum
description: This is a revision of UCSF/Sil lab-Histoplasma capsulatum-G217Bprint2 (GEO accession GPL6125) adding 15 oligos relative to the original design.  The new oligo IDs are prefixed with G217Borfs2.  G217Borfs2.38n17 targets MS8 (GenBank AF292398) which is incorrectly merged with HISTO_ZT.Contig181-snap.97 in the predicted gene set, and the remaining 14 target novel transcripts identified during preliminary analysis of whole genome tiling array data (published with a revised and expanded set of novel transcripts in BMC Microbiol. 11:216).
manufacture_protocol: Spotted using DeRisi arrayer in Center for Advanced Technology, Genentech Hall, Mission Bay.  70-mer oligonucleotides representing each predicted gene are resuspended in 3XSSC and spotted on poly-lysine slides purchased from Erie Scientific.