title: MIQE validated qPCR assays
status: Public on Jun 17 2014
contact.city: Shanghai
contact.first: Leming
contact.last: Shi
contact.email: lemingshi@fudan.edu.cn
contact.address: 2005 Songhu Road
contact.department: School of Life Sciences
contact.country: China
contact.institute: Fudan University
contact.phone: +86-18616827008
organism: Homo sapiens
description: PCR assay; total volume: 5 μl; instrument: CFX384 (with automation); mastermix: SsoAdvanced SYBR; primer concentration: 250 nM each
  Samples: cDNA: 25 ng (total RNA equivalents); gDNA: 2.5 ng (Roche); NTC: water + carrier (5 ng/μl yeast transfer RNA); synthetic template (pooled 60-mers in concentration range: 2E7 – 2E1 copies)
  PCR program: default cycling protocol for SsoAdvanced SYBR (Ta=60°C)
manufacture_protocol: see manufacturer's website