title: Gonococcal antigens microarray
status: Public on Aug 15 2024
contact.city: Manchester
contact.first: Fidel
contact.last: Ramirez Bencomo
contact.email: fidel.ramirezbencomo@manchester.ac.uk
contact.state: England
contact.address: Michael Smith Building
contact.department: FBMH/SBS/EIGen
contact.country: United Kingdom
contact.institute: The University of Manchester
organism: Neisseria gonorrhoeae
manufacture_protocol: Purified recombinant proteins were diluted to a final concentration of 0.5 mg/ml combined with JetStar printing buffer (in a 1:1 ratio), which has been optimised to produce high-quality features. Each drop dispensed by an Arrayjet microarrayer is 100pL. A deposition volume of 400pL (4 drops per spot) was printed onto a 16-pad ONCYTE SuperNOVA (Grace Bio-Labs) nitrocellulose slide ) using the non-contact printer Arrayjet Marathon (Arrayjet, Edinburgh, UK). The process was carried out at around 55% relative humidity (RH) and 10°C. The temperature for printing was changed from 18°C to 10°C to maintain the stability of the samples. Each protein was printed in 5 replicates on each microarray. The printed slides were incubated in the arrayer at 10°C overnight and stored at 4°C.  The samples were combined with JetStar™ printing buffer (in a 1:1 ratio) which has been optimised to produce high quality features.  University of Manchester provided all relevant information in relation to storage and handling of the samples including materials safety data sheet (MSDS) if applicable.