title: Mouse 33K cDNA array
status: Public on Sep 20 2010
contact.city: Roma
contact.first: Francesca
contact.last: Amati
contact.email: amati@med.uniroma2.it
contact.address: Via Montpellier 1
contact.department: Biomedicine and Prevention
contact.country: Italy
contact.institute: University of Rome Tor Vergata
contact.phone: +39-6-72596080
organism: Mus musculus
manufacture_protocol: The arrays are printed under rigorous clean-room conditions and quality control is employed all along the way, starting with preparation of the printing material as well as during the actual printing and finally with post-printing tests that include a sample chip hybridization. All cDNA products are tested on Agarose gel to assure quality and concentration of PCR products. During array printing, sample slides are inspected to assure proper performance of pen tips. After printing sample slides are dye stained to help quantitate the concentration of the deposited products and then a test hybridization is performed. Print plates are barcode scanned and a print file is computer generated while printing to assure error-free spot ID. Each slide is uniquely numbered so as to permit documented traceability starting from the manufacturing batch of the blank slide, to the printing location while on the printer, to the environmental conditions during printing and actual content spotted, to the individual who purchased the array & performed the hybridization and submitted it for scanning.  Incorporated into all of our standard arrays is a set of bacterial DNA spots that can be used to calibrate hybridization results. Four bacterial DNA spots are printed in each pen domain of every chip as a minimum. These spots can be used as negative controls as is, or, if appropriate bacterial DNA is spiked into each of the probe samples, can be used to equilibrate ratios for each pen domain, or for ratio gain adjustment when spiked in appropriate ratios. The Oligo arrays have an additional set of 384 control spots consisting of 12 positive and 12 negative controls each replicated 16 times.