title: MRC Toxicology Unit Mus musculus 23k MEEBO probe set slide A
status: Public on Jun 21 2012
contact.city: Leicester
contact.first: Kate
contact.middle: Marie
contact.last: Dudek
contact.email: kmp15@le.ac.uk
contact.address: Hodgkin Building, Lancaster Road
contact.department: Genomics Group
contact.country: United Kingdom
contact.institute: MRC Toxicology Unit
contact.phone: +44 (0)116 252 5582
organism: Mus musculus
manufacture_protocol: This is a new version of platform number GPL9106. 70-mer oligos (Invitrogen MEEBO probe set-originally Illumina) are supplied dried down at 200 pmol concentration in Genetix 384 well plates. The full printing set consists of 101 plates. Prior to printing Oligos are resuspended in 3 x SSC, 1.5 M Betaine, 30% ethylene glycol. Plates are loaded onto an ArrayJet microarrayer 48 at a time; the slides used are Genetix aldehyde plus. Printing is performed using inkjet-style technology to deposit oligo on each slide. The software requests the addition of the next batch of plates once the first 48 have been printed. The layout of the print is 3 blocks, each one consisting of 32 columns and 216 rows. We also included a marker plate top and bottom to make aligning the features easier. The entire print run is split over 2 slides (A and B) due to space constraints.