title: DrexelMed-Plasmodium yoelii-7K-Version 2.0 [condensed version]
status: Public on Nov 01 2011
contact.city: Philadelphia
contact.first: James
contact.middle: M.
contact.last: Burns
contact.email: jburns@drexelmed.edu
contact.state: PA
contact.address: 2900 Queen Lane
contact.department: Microbiology and Immunology
contact.country: USA
contact.institute: Drexel University College of Medicine
contact.phone: 215-991-8490
organism: Plasmodium yoelii
manufacture_protocol: 1. Oligonucleotides with a concentration of 80 uM in dH20, from Illumina, Inc., were originally arrayed from 96-well plates into MJ Research 384-well flat-bottom plates.  Each well received an equal volume of 6X SSC so that each 65-mer oligo was a final concentration of 40 uM in 3X SSC.  Plates were then dried by SpeedVac, sealed and stored at 4C.
  2. One day prior to printing, oligos were rehydrated to 40uM with sterile de-ionized water, sealed with ABGene adhesive films and incubated overnight in a humid environment with continuous agitation on a flat-bed shaker.
  3. The next day, plates were centrifuged at 2K rpm for 1 min. and then stored at 4C.
  4. Each plate, as needed, was unsealed and placed onto the plate holder of a Brown Lab (Stanford) style 32-pin array printer and the oligos spotted.
  5. Following printing, each plate was re-sealed and stored at 4°C until re-dried and re-sealed as above.
  6. For subsequent printings, oligos were resuspended in 0.5ul less sterile de-ionized water to compensate for the 0.5ul removed during the previous printing.