title: MIT S.cerevisiae ORF 15K version 4.2
status: Public on Mar 18 2008
contact.city: Cambridge
contact.first: Joel
contact.last: Moxley
contact.email: jfmoxley@mit.edu
contact.state: MA
contact.address: 77 Massachusettes Ave
contact.country: USA
contact.institute: MIT
organism: Saccharomyces cerevisiae
description: Array with 15488 features, in 32 zones, with 12558 biosequences representing 14966 reporters and 0 composite sequences, with surface type polylysine
manufacture_protocol: Microarrays were spotted on UltraGAPS II slides (Corning) using an OmniGrid 100 microarrayer (GeneMachines). After spotting, arrays were baked at 80°C for 2 hours, cross-linked at 300 mJ in a UV Stratalinker 2400 (Stratagene), and stored under vacuum. Hybridizations were conducted at 42°C for 15 hours using the Lucidea SlidePro automated hybridization machine (Amersham Biosciences).  Described in A-MEXP-217.mageml.tgz