title: 25K human oligonucleotide array v2
status: Public on Dec 21 2012
contact.city: Illkirch
contact.first: Doulaye
contact.last: Dembele
contact.email: doulaye@igbmc.fr
contact.address: 1 rue Laurent Fries
contact.department: Biopuces
contact.country: France
contact.institute: IGBMC
contact.phone: +33 3 88 65 35 28
organism: Homo sapiens
manufacture_protocol: The 50 mers 5’amino modified oligonucleotides from the RNG/MRC oligonucleotide collection (see Le Brigand et al, below) (information available at http://www.microarray.fr:8080/merge/index) were diluted to a final concentration of 50 mM in 50% dimethyl sulfoxide (D-8418, Sigma), 100mM potassium phosphate (pH 8.0) and printed onto hydrogel-coated slides (Nexterion H slides, Schott, Jena, Germany) using a microGrid II arrayer (Genomic Solutions, Cambridge, UK).
  Le Brigand K, Russel R, Moreillon C, et al. An open-access long oligonucleotide microarray resource for analysis of the human and mouse transcriptomes. Nucleic Acids Research, 2006 Jul 19; 34(12):e87