title: Luminex xMAP Multi-Analyte Profiling ProcartaPlex 65 plex
status: Public on May 09 2025
contact.city: Portland
contact.first: Lisa
contact.last: Coussens
contact.email: coussensl@ohsu.edu
contact.state: OR
contact.address: 3181 SW Sam Jackson Park Rd
contact.country: USA
contact.institute: Oregon Health and Science University
organism: Homo sapiens
manufacture_protocol: Vortex capture beads for 30 sec. Add 50 uL of capture beads to each well. Remove liquid. Add 25 uL of Universal Assay Buffer, then add 25 uL of standards, controls, or samples. For background wells, add 50 uL of 1X UAB. Seal plate and incubate with shaking at room temp for 2 hr. Add 25 uL of Detection Antibody Mix (1X). Seal the plate and incubate with shaking at room temp for 30 min. Wash plate twice. Add 50 uL of Streptavidin-PE. Seal the plate and incubate with shaking at room temp for 30 min. Wash plate twice. Add 120 uL of Reading Buffer. Seal the plate and shake at room temp for 5 min. Aquire data on xMAP system.