title: University of Cambridge/Bead-based microRNA profiling platform version 4
status: Public on Jun 30 2009
contact.city: Darlinghurst
contact.first: Leonard
contact.last: Goldstein
contact.address: 384 Victoria St
contact.country: Australia
contact.institute: Garvan Institute of Medical Research
organism: Mus musculus
manufacture_protocol: The expression of 318 unique murine miRNAs was determined using a bead-based flow-cytometric profiling platform as described previously (Lu et al. Nature 2005, Blenkiron et al. Genome Biology 2007). The platform was adapted to include 334 probes for 318 unique murine miRNAs based on the miRNA repository miRBase 9.1 as well as probes for six artificial control RNAs. In brief, oligonucleotide capture probes complementary to the miRNAs of interest were conjugated to colour coded polystyrene beads (xMAP beads, Luminex Corporation, Austin, TX, USA) allowing the simultaneous detection of 90 different target miRNAs.