title: Human_Cancer MicroRNA Array_v1.0
status: Public on Sep 11 2010
contact.city: Chicago
contact.first: Jingjing
contact.last: Wu
contact.email: wu_jingjing2007@yahoo.com.cn
contact.state: IL
contact.address: 303 E. Chicago Avenue
contact.department: pathology
contact.country: USA
contact.institute: northwestern university
contact.phone: 847-530-3863
organism: Homo sapiens
manufacture_protocol: In this array, each miRNA is targeted by two oligos, each that hybridizes a half molecule of miRNA to form a RNA/DNA duplex. After target miRNA and oligos are matched, the joint is ligated by DNA ligase. Then the ligated molecules are amplificated via T7 trancription into RNA in the presence of biotin-UTP which can be used for hybridization.