title: Micro-CRIBI Mouse 29K Oligo Array (Operon V1.1)
status: Public on Dec 31 2007
contact.city: Padova
contact.first: Gerolamo
contact.last: Lanfranchi
contact.email: stefano.cagnin@unipd.it
contact.address: Via U. Bassi, 58/B
contact.department: CRIBI - Biotechnology Center and Biology Department
contact.country: Italy
contact.institute: University of Padova
contact.phone: +39-0498276219
organism: Mus musculus
manufacture_protocol: This set consists of 13,443 70mer oligonucleotides (Operon version 1.1) designed on Mouse Unigene clusters, mainly in the 3'-terminal region. Each oligonucleotide is spotted by our robotic station Biorobotics Microgrid II, in two replicates on a MICROMAX glass slide SuperChip I (Cat No MPS696) provided by PerkinElmer Life Sciences Inc. The deposition is qualitatively assessed by a scan of the spotted slide at 10 micron resolution, using a GSI Lumonics scanner and a protocol that allows the detection of spotted features through the salts derived from the dried solution in which oligonucleotides are dissolved. Microarray slides that meet the quality standards, are washed as recommended by the supplier and the glass slides are stored, ready for use, in the dark under deep vacuum. Click below to see images of the array and detailed description of the spotting geometry, with the necessary parameters for analysis.