title: PIQOR Immunology Microarray, Mouse CTID619
status: Public on Mar 31 2007
contact.city: Cologne
contact.first: Sebastian
contact.last: Knoebel
contact.email: sebastian@miltenyibiotec.de
contact.address: Nattermannallee 1
contact.country: Germany
contact.web_link: http://www.miltenyibiotec.com
contact.institute: MiltenyiBiotec GmbH
contact.phone: 0221-95048220
organism: Mus musculus
description: The PIQOR Immunology Microarray is composed of 1076 mouse specific cDNAs. These cDNAs include for each array six house-keeping genes as positive controls. In addition four E. coli DNA fragments and the corresponding in vitro transcribed RNAs (spiking controls) provide a control for the labeling and hybridisation efficiency. Two negative controls are given by spotted herring sperm DNA and buffer. Genes are spotted in quadruplicates.
manufacture_protocol: Defined 200-400 bp fragments of selected cDNAs were generated by RT-PCR, cloned and sequence-verified. Amplified and purified cDNA inserts were spotted in quadruplicate on PIQOR slides using a proprietary piezoelectric spotter. The PIQOR slides were coated in a 2 step process generating a surface which covalently binds DNA molecules.