title: Human 30K 60-mer oligo array
status: Public on Aug 16 2007
contact.city: London
contact.first: Andrew
contact.middle: E
contact.last: Teschendorff
contact.email: a.teschendorff@ucl.ac.uk
contact.address: 72 Huntley Street
contact.department: UCL Cancer Institute/Institute for Women's Health
contact.country: United Kingdom
contact.institute: University College London
contact.phone: +44 02076790727
organism: Homo sapiens
description: hydrophilic polymer containing N-hydroxysuccinimide (NHS) ester reactive groups
manufacture_protocol: The Human Release 2.0 oligonucleotide library, containing 60mer oligonucleotides representing 28830 unique genes as designed by Compugen (San Jose, CA, USA), was obtained from Sigma-Genosys (Zwijndrecht, The Netherlands). The oligonucleotides were dissolved at 10 microM concentration in 50 mM sodium phosphate buffer pH 8.5 and single spotted onto CodeLink slides (Amersham Biosciences), using an OmniGrid 100 microarrayer (Genomic Solutions, Ann Arbor, MI, USA) equipped with SMP3 pins (TeleChem International, Sunnyvale, CA, USA). After printing slides were processed and blocked prior to use according to the manufacturer's protocol.