title: SGDB Yeast 15.36K Operon
status: Public on Feb 17 2012
contact.city: Prague
contact.first: Michal
contact.last: Cap
contact.email: michal_cap@volny.cz
contact.address: Vinicna 5
contact.department: genetics and microbiology
contact.country: Czech Republic
contact.institute: Faculty of Science, Charles University in Prague
organism: Saccharomyces cerevisiae
manufacture_protocol: Slide spotted using BioRobotics spotting machine and arrayer software TAS aplication suite  MicroGrid II v 2.2.2.3 (Genomic Solution)
  Spotting conditions: Temperature of 20¡C more or less 5¡C. Hygroscopy of 50% more or less 5%.
  Before each spotting:
  1. Printing tips (Geneworx 10k) are tested on glass slides.
  2. The spotting robot is put in operation and water tanks filled with distilled water.
  3. Corning glass slides are deposited on plates.
  4. The spotting program specific for each slide bacth is launched.
  For spotting oligonucleotides from Operon Yeast Genome Oligo Set version 1.1.2 (Eurofins MWG Operon) are used
  After the spotting process, spots of the first and last slides are visualized using SYBR Green labelling.