title: Univ Freiburg Sinorhizobium meliloti Sm6kPCR
status: Public on May 20 2010
contact.city: Napoli
contact.first: Carmen
contact.last: Bianco
contact.email: bianco@igb.cnr.it
contact.address: Via p. Castellino 111
contact.country: Italy
contact.institute: IGB-CNR
organism: Sinorhizobium meliloti 1021
manufacture_protocol: PCR fragments and oligonucleotides were printed onto 3-aminopropyltrimethoxysilane-coated SA-1 glass slides (Asper Biotech, Tartu, Estonia) using the MicroGrid II 600 spotter (BioRobotics, Cambridge, UK) equipped with 48 SMP3 stealth pins (TeleChem International, Sunnyvale, CA, USA). DNA was cross-linked to the surface by incubation of the slides for 3 h at 80◦ C. Unbound DNA was removed by two washes in 0.1% (w/v) SDS for 2 min at 42◦C and two washes in distilled water for 2 min at 20◦C. After denaturation at 100◦ C for 3 min in distilled water, slides were dunked into ethanol and dried by centrifugation.