title: BFK 20 DNA microarray
status: Public on Jan 13 2006
contact.city: Bratislava 45
contact.first: Tomas
contact.last: Majtan
contact.email: tomas.majtan@savba.sk
contact.address: Dubravska cesta 21
contact.department: Department of Molecular Genomics
contact.country: Slovakia
contact.web_link: http://imb.savba.sk/~majtan/
contact.institute: Institute of Molecular Biology SAS
contact.phone: +421-2-59307429
organism: Corynebacterium phage BFK20
manufacture_protocol: Probes were PCR amplified using gene-specific primers. Double stranded PCR products were purified using QIAquick PCR Purification Kit (QIAGEN), eluted into the sterile water, quantified spectrophotometrically and lyophilized. Probe DNA was subsequently dissolved in 50 % DMSO as spoting solution in 200 ng/ul. The 12 ul was tranfered into 384-well spoting microplate and spotted in 4 replicates onto GAPS slides (CORNING) using PixSys5500 microarrayer (CARTESIAN TECHNOLOGIES) equipped with CMP4 Micro Spotting pin (TELECHEM INTERNATIONAL). Spoting conditions included 22°C temperature and 47% humidity. Slides were dried overnight and crosslinked by UV irradiation at 250 mJ.