title: Lachancea kluyveri microarray
status: Public on Jun 21 2013
contact.city: Lund
contact.first: Anna
contact.middle: Andersson
contact.last: Rasmussen
contact.email: anna.rasmussen@biol.lu.se
contact.address: Sölvegatan 23
contact.department: Molecular Cellbiology
contact.country: Sweden
contact.institute: Lund University
contact.phone: 0462221547
organism: Lachancea kluyveri
description: Please note that, at the time the platform was designed, searchable accession numbers were not available for L. kluyveri. However, all oligos have exact matches in the Genolevures database (http://genolevures.org/), ie by blasting against it, and selecting Saccharomyces kluyveri as the organism (i.e.the former name of L. kluyveri) will give only one 100% hit, and thus tell exactly which gene matches each oligo.
manufacture_protocol: The oligonucleotides (20µM  in 3x Saline Sodium Citrate (SSC) buffer with 0.75M betaine) were printed in triplicate on epoxy coated glass slides (Corning) using a linear servo arrayer (P. Brown; Stanford University, made after the DeRisi model, http://derisilabs.ucsf.edu/). The printed slides were placed in at room temperature oven at 70% humidity for 12-14 hours.  After incubation, the slides were cross-linked using the UV Stratalinker 2400 (Stratagene) at 150 microjoules/cm2.  The processed slides were placed in a light-proof slide box and stored in a dessicant cabinet for future hybridization experiments.