title: BMFZ-GTL_Mmu_40K_v3
status: Public on Nov 30 2017
contact.city: Duesseldorf
contact.first: Karl
contact.last: Koehrer
contact.email: rene.deenen@hhu.de
contact.address: Universitaetsstr. 1
contact.department: BMFZ
contact.country: Germany
contact.institute: University of Duesseldorf
organism: Mus musculus
manufacture_protocol: Oligos were purchased from Operon (AROS Mus Musculus V.4.0, plates 1-95 + control plate and buffer plate) arrayed in Genetix V-bottom 384-well plates. Each well contained 300 pmol of 70-mer oligo. A day before printing oligos were resuspended in 15 µl Amino-Spotting-Buffer (Genetix Ltd., Hampshire, UK). Plates were centrifuged at 1000 rpm for 5 minutes, sealed and stored at 8°C over night. Before spotting, plates were centrifuged at 1000 rpm for 5 minutes one more time. Seals were removed, plates covered with plate lids and placed in appropriate location of the plate cassette. Oligonucleotides were spotted to aminosilane coated UltraGap microarray slides (Corning Inc., NY, USA) using a QArray2 microarray spotter (Genetix Ltd., Hampshire, UK). After completion of the print run, plates were dried through passive evaporation in a protected environment.