title: LC Sciences MRA-1038 Plants miRNA Array (miRBase 16)
status: Public on Jan 01 2014
contact.city: Hangzhou
contact.first: Bo
contact.last: Wang
contact.email: wang_bo1986@hotmail.com
contact.state: zhejiang
contact.address: Xiasha
contact.country: China
contact.institute: Zhejiang Sci-Tech University
contact.phone: +86-133 8653 9918
organism: synthetic construct
manufacture_protocol: Arrays are made based µParaflo microfluidic technology (Atactic Technologies). On the µParaflo microfluidic chip, each detection probe consists of a chemically modified nucleotide coding segment complementary to target microRNA (from miRBase, http://microrna.sanger.ac.uk/sequences/) or other RNA (control or customer defined sequences) and a spacer segment of polyethylene glycol to extend the coding segment away from the substrate. The detection probes are made by in situ synthesis using PGR (photogenerated reagent) chemistry.