title: Laval/Sirard bovine embryo 3K V3.0
status: Public on Dec 15 2010
contact.city: Québec City
contact.first: Marc-André
contact.last: Sirard
contact.email: Marc-Andre.Sirard@fsaa.ulaval.ca
contact.state: Quebec
contact.address: Offfice 2732, 2440 Hochelaga Blvd.
contact.department: Sciences Animales
contact.country: Canada
contact.institute: Université Laval
organism: Bos taurus
manufacture_protocol: cDNA was obtained by SSH experiments (Clontech). The substractions were done on embryos from GV stage to Blastocyst stage. Each clone was amplified by PCR, purified, dry-down and resuspended in H20/DMSO(50:50). The clones were spotted using a chipwritter (Virtek) on ultra-glass slides (Corning). The slides were UV treated occording to the manufacturer's protocole.