title: LC_MRA-1001_miRHuman_12.0_080901
status: Public on Sep 10 2010
contact.city: North Chicago
contact.first: Dominik
contact.middle: M
contact.last: Duelli
contact.email: dominik.duelli@rosalindfranklin.edu
contact.state: IL
contact.address: 3333 Green Bay Road
contact.department: Pathology
contact.country: USA
contact.institute: Rosalind Franklin University of Medicine and Science
contact.phone: 847 578 8341
organism: Homo sapiens
manufacture_protocol: Arrays are made based µParaflo microfluidic technology (Atactic Technologies). On the µParaflo microfluidic chip, each detection probe consists of a chemically modified nucleotide coding segment complementary to target microRNA (from miRBase, http://microrna.sanger.ac.uk/sequences/) or other RNA (control or customer defined sequences) and a spacer segment of polyethylene glycol to extend the coding segment away from the substrate. The detection probes are made by in situ synthesis using PGR (photogenerated reagent) chemistry.