title: Arabidopsis thaliana (ecotype Columbia) chromosome 4 knob genomic tiling array
status: Public on Mar 31 2004
contact.city: Austin
contact.first: Matthew
contact.middle: Wayne
contact.last: Vaughn
contact.email: vaughn@tacc.utexas.edu
contact.state: TX
contact.address: 10100 Burnet Rd
contact.department: Texas Advanced Computing Center
contact.country: USA
contact.institute: University of Texas at Austin
contact.phone: (512) 232-7124
organism: Arabidopsis thaliana
description: This array includes 1724 PCR products, mostly 1kb long, designed to cover sequentially a 1.5 Mb region 1201322-2673088 on the short arm of chromosome 4, plus an additional thirty-six 10kb regions located on the 5 chromosomes. As spots are printed in duplicate or triplicate on the slide, the total number of features is 5743.
  PCR products are amplified from BACs or genomic DNA, purified, and spotted (100-120 um average diameter) on 25x75 mm aminosilane-coated Corning CMT-GAPS II glass slides
  Most features are spotted more than once. Replicate spots are identified by an _[0-9] appended to the end of the feature ID. For example, pc13a09F.1044.4.2387459.T4B21_1 is the first instance of the feature pc13a09F.1044.4.2387459.T4B21.
  PCR products are printed in 3xSSC
  Printed slides are plunged in boiling water for 5' to denature PCR products before hybridization.
  Keywords = genomic
  Keywords = tiling
  Keywords = PCR product