title: Rat microRNA array
status: Public on May 07 2014
contact.city: Stillwater
contact.first: CHAOQUN
contact.last: HUANG
contact.email: chaoqh@okstate.edu
contact.state: OK
contact.address: 264 McElroy Hall
contact.department: Physiological Sciences
contact.country: USA
contact.institute: Oklahoma State University
contact.phone: 405-744-3410
organism: Rattus norvegicus
manufacture_protocol: Probe sets, which contained 5' amino modified C6 oligos were synthesized by Sigma-Genosys (Woodlands, TX) at 100 µM concentration and suspended in 3 × SSC buffer. The oligos were diluted to 25 µM with 3 × SSC prior to use. The probes were then printed onto epoxy-coated slides (CEL Associates, Pearland, TX) with an OmniGrid 100 array (GeneMachine, San Carlos, CA) at 65% humidity and then incubated for 48 hours at the same humidity. Each slide contained three identical blocks in a landscape orientation. Within each block every probe was printed 6 times in 3 separate pairs. The oligo set also contained one probe for U6 and 3 probes for tRNAs as positive controls as well as one probe for a plant miRNA as a negative control.