title: Streptococcus gordonii Challis whole genome array SG-1
status: Public on Oct 30 2007
contact.city: Buffalo
contact.first: M
contact.middle: M
contact.last: Vickerman
contact.email: mmv4@buffalo.edu
contact.state: NY
contact.address: 3435 Main St.
contact.department: Oral Biology
contact.country: USA
contact.institute: University at Buffalo
organism: Streptococcus gordonii str. Challis
manufacture_protocol: Unique 70-mer oligonucleotides were identified for each of the 2,186 open reading
  frames (ORFs) identified in the initial annotation of the Streptococcus gordonii genome.  Oligonucleotides were resuspended at 25 uM final concentration in Pronto universal spotting solution (Corning #40027) and printed onto bar-coded UltraGAPs Coated Slides (Corning #40015) using an Automated Microarraying System from Brooks Automation, Inc. (Chelmsford, MA) equipped with a 48 pin printhead containing Stealth 3 MicroSpotting Pins Telechem International, Inc.).  Print configuration is 48 blocks in a 4 X 12 arrangement.  Spot diameters are approximately 90-100 microns.  Control reporters included 10 Arabidopsis gene fragments.   After printing, oligonucleotides were cross-linked to the slides by UV irradiation at 250 mJ/cm2 (Stratalinker UV Crosslinker, Stratagene) and subsequently stored at room temperature in a desiccated chamber until use.