title: SYBR green based Human miRNA PCR assay panel
status: Public on May 01 2013
contact.city: Shenyang
contact.first: zining
contact.last: zhang
contact.email: zi_ning101@hotmail.com
contact.state: Liaoning
contact.address: No 155, Nanjingbei street, Heping District
contact.department: 1st affiliated hospital
contact.country: China
contact.institute: China Medical University
organism: Homo sapiens
description: This is a SYBR green based qPCR method to achieve higher accuracy and sensitivity of small RNA detection. E. Coli polyA polymerase was employed to add multiple adenines at the end of every RNA molecules in the total RNA pool that lacks of a polyA tail. With retro-transcriptase and oligodT annealing, a universal tag was introduced to the 3’ end of cDNAs. Using this tag, quantitative PCR was performed with microRNA-specific forward primer and reverse universal primer mix.
manufacture_protocol: n/a