title: PHAC_Booth_Lab_miRNA_microarray_v.2006
status: Public on Sep 30 2008
contact.city: Winnipeg
contact.first: Reuben
contact.last: Saba
contact.email: Reuben_Saba@phac-aspc.gc.ca
contact.state: Manitoba
contact.address: 1015 Arligton Street
contact.department: Medical Microbiology
contact.country: Canada
contact.institute: University of Manitoba: Public Health agency of Canada
contact.phone: 204-789-2000 (ext 3053)
organism: Mus musculus
manufacture_protocol: A microarray containing oligonucleotide probes complimentary to published miRNAs was constructed. These probes consisted of all the non-redundant miRNA sequences from the miRNA registry (Release 8.2). The arrays were spotted in-house on Corning® epoxide coated slides; Probes were spotted in duplicate and additionally two full arrays were printed per slide. The probes were in sense orientation relative to the mature miRNAs and thus complimentary to either the cDNA or aRNA that would be derived from the mature miRNAs. The probes also had a 15 nucleotide long poly(T) tract at the 5'end for steric distancing from the array surface. Each probe was printed in duplicate and the signal intensities for averaged.