title: Yale University MCDB Medicago truncatula NimbleGen 390K Oligo Array
status: Public on Jan 15 2008
contact.city: New Haven
contact.first: Hang
contact.last: He
contact.email: hehang@gmail.com
contact.state: CT
contact.address: 165 Prospect St
contact.department: MCDB
contact.country: USA
contact.institute: Yale University
organism: Medicago truncatula
manufacture_protocol: 1. Oligo length: 36
  2. Minimum oligonucleotide Tm: 80
  3. Maximum oligonucleotide Tm: 95
  4. Temperature to use during secondary structure prediction (an oligo will be rejected if it can fold into a stable secondary structure at this temperature): 65
  5. Threshold to report putative cross-hybridizations (all targets hybridizing with a given oligo with a Tm exceeding this threshold are reported): 70
  6. Minimum oligonucleotide GC content: 40
  7. Maximum oligonucleotide GC content: 73 for gene models; 77 for TARs
  8. Minimum distance between the 5' end of two adjacent oligos: 1
  BAC fasta and description files linked as supplementary files