title: BOAC CattleOligo 13.2K + 8.7K 70-mer microarray v2
status: Public on Apr 09 2008
contact.city: Jouy-en-Josas
contact.first: Sylvain
contact.last: Marthey
contact.email: abridge@inra.fr
contact.address: Domaine de Vilvert Bat 320
contact.department: Animal Genetics
contact.country: France
contact.institute: INRA
organism: Bos taurus
description: spotted oligonucleotide
manufacture_protocol: 1-1. The oligos set used for this microarray is a mix of the 8.7k set from Operon and a 13.2K
  set designed by the Illinois University and synthetised by Illumina.
  1-2. Oligos are arrayed by Illumina in Greiner 384-well flat-bottom plates. Each well contains 3000 pmol of 70-mer oligo.
  1-3. Oligos are arrayed by Operon in Genetix 384-well V-bottom plates. Each well contains 600 pmol of 70-mer oligo.
  2. Resuspend oligos in water(20µM).
  3. Incubate at room temperature during a night shaking plates to aid resuspension of DNA.
  4. Deep freeze and defrost centrifuging plates at 1000 rpm for 16 minutes then 2000 rpm for 3 minutes.
  5. Take 5 µl of oligos solution and put it in a new genetix polypropylène v-bottom plates(cat# X7022)
  6. Add 5 µL of 1X Printing Buffer to each well and mix.
  7. Centrifuge plates at 2000 rpm for 3 minutes.
  8. Oligos are ready for spotting.
  9. Spotting on glass slides, with 48 pins on cheapwriter of Virtek, with control of humidity (45-50%).
  10. Fixation of the oligos slides with UV (600 mJoules).
  11. Storage the slides in a vacuum.
  12. After the print run is completed, cover oligos plates with seals and deepfreeze them at 20°C in a protected environment.