title: NCSU_P.furiosus_COM1_hybrid_array
status: Public on Mar 07 2015
contact.city: Raleigh
contact.first: Aaron
contact.last: Hawkins
contact.state: NC
contact.address: 840 Main Campus Dr
contact.department: Chemical Engineering
contact.country: USA
contact.institute: North Carolina State University
organism: Pyrococcus furiosus COM1
manufacture_protocol: A DNA microarray was designed and constructed based on at least 2032 protein-coding sequences in the P. furiosus COM1 genome obtained from the Department on Energy?s Joint Genomes Institute (http://genome.ornl.gov/microbial/msed) plus over 100 protein-encoding sequences from other thermophilic microorganisms. OligoArray 2.1 (Rouillard, 2003) was used to generate at least one 60-mer oligonucleotide probe sequence for each open reading frame. The probes were synthesized (Integrated DNA Technologies, IA), re-suspended in 50% DMSO, and printed onto Ultragap microarray slides (Corning, NY) using a QArrayMini arrayer (Genetix, UK). Each probe was spotted five times onto each array to fortify statistical analysis.