title: Toxoplasma gondii ChIP-chip genome-wide tiling array
status: Public on Mar 19 2009
contact.city: LA TRONCHE
contact.first: Mohamed-ali
contact.last: HAKIMI
contact.email: mohamed-ali.hakimi@univ-grenoble-alpes.fr
contact.state: Grenoble
contact.address: Domaine de la Merci, Campus Santé
contact.department: UMR5309
contact.country: France
contact.institute: CNRS
contact.phone: (33)476637469
organism: Toxoplasma gondii ME49
description: Tiling microarray encompassing 65 Mb of Toxoplasma gondii (strain ME49) genome (chromosomes Ia, Ib, II, III, IV, V, VIIa, VIIb, VIII, IX, X, XI and XII). RefSeq genome accession: NZ_ABPA00000000.1.
  50mer probes selected at 82 bp interval spacing
  From : TOXODB release 4.0
  Genomic sequence available at: http://toxodb.org/common/downloads/release-4.0/
  Native NimbleGen files (ndf, pos) are linked below as supplementary files.
manufacture_protocol: NimbleGen manufactures custom, high-density DNA arrays based on its proprietary Maskless Array Synthesizer (MAS) technology. The MAS system is a bench top, solid-state, high-density DNA array fabrication instrument comprised of a maskless light projector, a reaction chamber, a personal computer, and a DNA synthesizer. NimbleGen builds its arrays using photo-mediated synthesis chemistry with its MAS system.
  At the heart of the system is a Digital Micromirror Device (DMD), similar to Texas Instruments' Digital Light Processor (DLP), employing a solid-state array of miniature aluminum mirrors to pattern up to 786,000 individual pixels of light. The DMD creates "virtual masks" that replace the physical chromium masks used in traditional arrays.
  These "virtual masks" reflect the desired pattern of UV light with individually addressable aluminum mirrors controlled by the computer. The DMD controls the pattern of UV light projected on the microscope slide in the reaction chamber, which is coupled to the DNA synthesizer. The UV light selectively cleaves a UV-labile protecting group at the precise location where the next nucleotide will be coupled. The patterns are coordinated with the DNA synthesis chemistry in a parallel, combinatorial manner such that up to 786,000 unique probe features are synthesized in a single array.