title: UPenn Mouse PancChip 6.1
status: Public on Aug 16 2012
contact.city: Columbus
contact.first: Peter
contact.last: White
contact.email: Peter.White@nationwidechildrens.org
contact.state: OH
contact.address: 700 Children's Drive
contact.department: Microbial Pathogenesis
contact.country: USA
contact.institute: Nationwide Children's Hospital
organism: Mus musculus
manufacture_protocol: Bacterial clones were grown overnight in LB-AMP cultures.  Plasmid DNA was prepared for each clone using a Qiagen 3000 robot, and the purified DNA was used as a template to amplify the inserts with PCR. The clones were assigned a PCR score of 0 indicating a single band, 1 if there were no bands present, or 2 if there were multiple bands. The PCR products were purified, eluted with deioinzed sterile water, diluted with an equal volume of DMSO (Sigma) and printed on Corning ULTRA Gaps slides with a NanoPrint Microarrayer.   PancChip 6.1 contains 13,059 clones, many of which were previously included on the PancChip 5.0, and various control spots.