title: Mouse miRNA chip
status: Public on Oct 09 2004
contact.city: Toronto
contact.first: Tomas
contact.last: Babak
contact.email: tomas_babak@merck.com, tomas.babak@utoronto.ca
contact.state: ON
contact.address: 112 College St
contact.department: Banting and Best Department of Medical Research
contact.country: Canada
contact.institute: Best Institute
contact.phone: 416-978-1839
organism: Mus musculus
description: We describe a method to detect miRNAs using microarrays, in which the miRNAs are directly hybridized to the array. We used this method to analyze miRNA expression across 16 mouse organs and tissues. More than half of the 78 miRNAs detected were expressed in specific adult tissues.  By comparing miRNA levels to mRNA levels determined in a parallel microarray analysis of the same tissues, we found that the expression of target mRNAs predicted on the basis of sequence similarity is biased towards the tissues in which the corresponding miRNA is expressed.
  
  The data shown here includes data for 361 other ncRNAs as positive controls.  Probes were generated by tiling across each transcript, including 100 nucleotides of flanking sequence extracted from genomic sequence.
  Keywords = miRNA mouse