title: Invitrogen/INIA 96 qRT-PCR trout infection VHSV_NV
status: Public on Aug 14 2015
contact.city: Madrid
contact.first: Julio
contact.middle: M
contact.last: Coll
contact.email: juliocoll@inia.es
contact.state: Madrid
contact.address: crt Coruna km7
contact.department: Biotechnology
contact.country: Spain
contact.institute: INIA
contact.phone: 3413476850
organism: Oncorhynchus mykiss
description: Expression of genes after a viral hemorrhagic septicemia virus (VHSV) challenge.
  
  PCR assay
manufacture_protocol: Suitable primers were selected by the NCBI Primer-BLAST utility (http://www.ncbi.nlm.nih.gov/tools/primer-blast/). q-PCR plates were manufactured by Invitrogen (Life Technologies)  (http://www.invitrogen.com/site/us/en/home.html). RT_PCR assays were performed at INIA facilities in a 7500 Fast Real-Time PCR System (Applied Biosystems).