title: Sequence Validated Human cDNA library by ResGen (Invitrogen) [print version 1]
status: Public on Aug 13 2013
contact.city: Grand Rapids
contact.first: Sok Kean
contact.last: Khoo
contact.state: MI
contact.address: 333 Bostwick NE
contact.country: USA
contact.institute: Van Andel Institute
organism: Homo sapiens
manufacture_protocol: 1.cDNA probes are stored in Corning 96-well microtiter plates. Each well contains 1,000 pmol of cDNA clone.
  2. Resuspend cDNA probes in PBS to 100 uM and rearray 100 µL into 384-well (Genetix microarray plate cat# 7022).
  3. Seal plates with Corning seals and store at -80 °C until print.
  4. For printing process, remove seals and cover with plate lids. Place in appropriate location of plate cassette. Plate lids are removed just before print run is started to minimize evaporation time before printing.  All arrays are printed on Corning CmT-Gaps2 amino-silane coated slides.
  5. After the print run is completed, allow slides to dry at room temp before vacuum sealed and store at 4 °C.
  6. For each subsequent preparation of a print run, wash microarray quill pins with PBS to remove residues.