title: Montastraea faveolata 1.5K v1
status: Public on Oct 31 2012
contact.city: Poughkeepsie
contact.first: Jodi
contact.last: Schwarz
contact.address: 124 Raymond Ave
contact.country: USA
contact.institute: Vassar College
organism: Orbicella faveolata
description: spotted amplified cDNAs
manufacture_protocol: 1.cDNAs are arrayed in Greiner 384-well flat-bottom plates.
  2. Resuspend  in water
  3. Allow Genetix plates to dry through passive water evaporation in a protected environment (e.g., chemical hood).
  4. Before printing, add 5 µL of 1X Printing Buffer to each well. This can be done the night before a print run is started.
  5. Seal plates with Corning seals.
  6. Incubate at 37°C for 30 minutes to aid resuspension of DNA.
  7. Shake plates near maximum rotational speed on flat-bed shaker for 1 minute.
  8. Centrifuge plates at 2000 rpm for 3 minutes.
  9. Remove seals and cover with plate lids. Place in appropriate location of plate cassette. This should be done with first plates just before print run is started to minimize evaporation time before printing. For second and third cassettes, wait until 30
  10. Make sure plates rest behind both holding clips in the cassettes. Push plates back into the cassettes as far as they will go, putting them in the proper position for the server arm.
  11. After the print run is completed, allow plates to dry through passive evaporation in a protected environment.
  12. For each subsequent preparation of these plates for a print run, add water to the wells instead of sodium phosphate buffer. The amount of water should be decreased by 0.25 µL per print run, as this is the amount drawn up by the pin capillary during ea