title: LC Human_Mouse_Rat miRNA Array (MRA-1030)
status: Public on Jun 23 2010
contact.city: Jefferson
contact.first: Zhiguang Li
contact.email: zhiguangli88@gmail.com, tao.chen@fda.hhs.gov
contact.state: AR
contact.address: HFT130, 3900 NCTR Road
contact.department: Division of Genetic an Reproductive Toxicology
contact.country: USA
contact.institute: National Center for Toxicological Research
contact.phone: 870-543-7295 870-543-7954
organism: Homo sapiens
  Mus musculus
  Rattus norvegicus
description: Name: HumanMouseRat miRNA Array
  Version: miRHumanMouseRat_12.0_080901
  Platform table annotations based on miRBase release 12.
manufacture_protocol: Arrays are made based µParaflo microfluidic technology (Atactic Technologies). On the microfluidic chip, each detection probe consisted of a chemically modified nucleotide coding segment complementary to target microRNA (from miRBase, http://microrna.sanger.ac.uk/sequences/) or other RNA (control or customer defined sequences) and a spacer segment of polyethylene glycol to extend the coding segment away from the substrate.  The detection probes were made by in situ synthesis using PGR (photogenerated reagent) chemistry.  The hybridization melting temperatures were balanced by chemical modifications of the detection probes.