title: GeneScan_SigB_350
status: Public on Mar 13 2012
contact.city: Greifswald
contact.first: Leif
contact.last: Steil
contact.address: Friedrich-Ludwig-Jahn Str. 15A
contact.department: Genetics and Functional Genomics
contact.country: Germany
contact.institute: University of Greifswald
organism: Bacillus subtilis
manufacture_protocol: ORF sequences of 350 selected genes from Bacillus subtilis were exported from Genbank. 50 nt long isothermal probes were derived from the ORF sequences and spotted in triplicate (GeneScan, Freiburg, Germany). Probes were covalently attached to a hydrophobic substrate on the surface of glass slides(Waldmüller et al., 2002).