title: Human forebrain organoid MERFISH
status: Public on Mar 29 2026
contact.city: Singapore
contact.first: Jinyue
contact.last: Liu
contact.email: liu_jinyue@gis.a-star.edu.sg
contact.address: 60 Biopolis Street
contact.country: Singapore
contact.institute: Genome Institute of Singapore
organism: synthetic construct
description: A Modified Hamming Distance 4 encoding scheme was adopted for MERFISH probe design. 30-nucleotide long target regions were identified using a previously published algorithm, with GRCh38 as reference, a specificity cut-off of 0.5 and isospecificity cut-off of 0.4. Three readout sequences per encoding probe were used; they were either 20-nucleotide long as published in Moffitt et al. , or 30-nucleotide long as published in Chen et al.
manufacture_protocol: REQUESTED