title: Galbraith Lab Maize Unigene cDNA microarray slide UG1-2.2
status: Public on Apr 19 2017
contact.city: Ithaca
contact.first: Tim
contact.middle: L.
contact.last: Setter
contact.email: tls1@cornell.edu
contact.state: NY
contact.address: 517 Bradfield Hall
contact.department: Crop & Soil Sci; Plant Breeding & Genetics
contact.country: USA
contact.institute: Cornell University
organism: Zea mays
manufacture_protocol: Microarray slides were printed using an OmniGridder (GeneMachines, San Carlos, California) and 8 Majer Precision pins (11077-1 Rev A MicroQuill 2000 Microarray Printing Tip, Majer Precision Engineering, Tempe, Arizona). Instrument settings were as follows: Dip time 500 milliseconds, Print time 0, After every Sample (Wash 2000ms, Dry 2000ms) 8 times, After every Plate (Sonicate 30,000ms, Wash 10,000ms, Dry 2000ms) 2 times-wash solution was 0.5 X SSC. Instrument settings were optimized prior to printing using a Cy3-labeled oligo to check print quality and verify no carryover between samples.