title: Agilent-024503 Rickettsia rickettsii str. Taiacu 15k v1.0
status: Public on Dec 01 2013
contact.city: São Paulo
contact.first: Maria
contact.middle: Fernanda
contact.last: Galletti
contact.email: mafegalletti@gmail.com
contact.state: São Paulo
contact.address: Av. Prof. Lineu Prestes, 1374 sala 39
contact.department: Department of Parasitology
contact.country: Brazil
contact.institute: Universidade de São Paulo - Instituto de Ciências Biomédicas - Departamento de Parasitologia
organism: Rickettsia rickettsii
description: Rickettsia microarray (15k chip, agilent)
  The complete genome sequence of the strain Sheila Smith of R. rickettsii (GenBank CP000848.1) was submitted to Agilent eArray web tool for gene expression probe (60mer) design (https://earray.chem.agilent.com/earray/)
  The total of 13,049 60-mer probes representing 99.5% of the bacterium genome (at least six probes per coding sequence) were spotted on an 8 x 15k chip
manufacture_protocol: We labeled, hybridized, and washed the arrays according to Agilent's Gene Expression FFPE adapted protocol.
  Two-color Microarray-Based Procaryote (version 1.2) FairPlay Labeling