title: NSB POSTECH DPS Mouse phosphokinase antibody array
status: Public on Feb 24 2015
contact.city: Mainz
contact.first: Hyobin
contact.last: Jeong
contact.email: h.jeong@imb-mainz.de
contact.state: Germany
contact.address: Richard shirrmann Str 12.
contact.country: Germany
contact.institute: IMB
organism: Mus musculus
description: Dendron-coated phosphokinase antibody array (DPA) with 88 spots of mouse antibodies.
manufacture_protocol: Dendron-modified glass slides (NSB27 NHS Slide) were preparedby using a standard (1 x 3) glass slide (Schott NEXTERION Glass B, ultrasonically cleaned, #1055132).). The slides were purchased (NSB POSTECH, Korea) and used for the phosphokinase antibody array. Antibodies in a printing buffer (0.01%BSA, 0.01% Tween 20, 0.5% glycerol in 1X PBS buffer (pH 8.5)) were spotted on the NHS-activated NSB27 slides by Genetix QArray-Mini microarray spotter. After the printing, the printed slides were incubated in a 70~85% humidified chamber for 5~12 hrs and then washed in 1XPBST (Sigma P3563-10PAK) and subsequently in 1X PBS (Sigma P4417-100TAB). Any chance of the printed slides getting dried in the process of washing was avoided.