title: Conway Institute Candida parapsilosis 3851 v1.0
status: Public on Mar 01 2007
contact.city: Dublin
contact.first: Geraldine
contact.last: Butler
contact.email: geraldine.butler@ucd.ie
contact.address: Belfield
contact.department: School of Biomolecular and Biomedical Science
contact.country: Ireland
contact.institute: University Colege Dublin Conway Institute
organism: Candida parapsilosis
manufacture_protocol: Oligonucleotides were designed from the genome sequence survey of C. parapsilosis CLIB214 (Logue, M. E., S. Wong, K. H. Wolfe, and G. Butler. 2005. A genome sequence survey shows that the pathogenic yeast Candida parapsilosis has a defective MTLa1 allele at Its Mating Type Locus. Eukaryot Cell 4:1009-1017).
  Oligonucleotides were synthesized by Operon Biotechnologies and resuspended in printing buffer from Genetix at a final concentration of 50 µM.  In total, 3851 oligonucleotides representing 3849 putative genes and the 2 positive controls were printed in duplicate on UltraGAPS coated slides (Corning) using a Q-array robot (Genetix) controlled with Qsoft software (Genetix). The general design is 2 subarrays of 16 blocks each, with all oligonucleotides spotted in duplicate, one copy per block. The slides were air dried for 2 days and DNA was cross-linked to the slide by UV at 6200 mJ cm-2 in an UV-linker (Stratagene). The slides were pre-soaked in a solution consisting of 75 % PBS, 25 % ethanol and 0.5% sodium borohydride (Alfa Aesar) for 4 h at 42ºC to remove potential spot autofluorescence. The slides were subsequently washed 3 times in water, 1 time in isopropanol, dried, and stored.