title: Miltenyi Biotec miRNA microarray (716)
status: Public on Jun 17 2007
contact.city: COLOGNE
contact.first: Frank
contact.last: Huebel
contact.email: frank.huebel@miltenyibiotec.de
contact.state: NRW
contact.address: Stoeckheimer Weg 1
contact.department: MACS Molecular 9
contact.country: Germany
contact.web_link: http://www.miltenyibiotec.com
contact.institute: Miltenyi Biotec GmbH, MACS Molecular Business Unit
organism: Homo sapiens
  Mus musculus
  Rattus norvegicus
description: Each microarray contained a total of 3540 DNA oligonucleotides with a sequence being reverse complement to the respective mature miRNA or control RNA, representing a total of 771 miRNA sequences and 114 controls with all probes spotted in quadruplicates. The 771 miRNAs sequences covered sequence verified miRNAs of the species human (494) mouse (383) and rat (352) as well as viral miRNAs (64) as described in the present study (http://www.mirz.unibas.ch/smiRNAdb). The 114 positive and negative control probes were chosen as follows. Three sets of controls, miRControl 1 (four sequences used to assess efficiency and sensitivity of labelling), miRControl 2 (single sequence used to assess efficiency of small RNA enrichment) and miRControl 3 (45 sequences used to normalize signal intensities across different samples and arrays) were artificial sequences chosen not be present in any of the human, mouse, rat or viral genome. A fourth set of positive controls presented 38 internal positive small but non-miRNA sequences including probes e.g. for 5.1 sRNA, tRNAs, and U6 RNAs. A fifth set of oligonucleotides included 26 negative controls like different single or double mismatch, antisense, or scrambled miRNA sequences.
manufacture_protocol: Microarrays were manufactured by Miltenyi Biotec. Briefly, 1.2 nl of 10 µM HPLC purified, 5’ amino-modified DNA oligonucleotides (Metabion) were spotted four times on aminopropyltrimethoxysilane / phenylendiisothiocyanate coated glass slides using a customized piezoelectric spotter.