title: VUMC MACF Mouse 38K oligo v64
status: Public on Oct 01 2007
contact.city: Amsterdam
contact.first: Daoud
contact.last: Sie
contact.email: d.sie@vumc.nl
contact.address: De Boelelaan 1117
contact.department: Pathology
contact.country: Netherlands
contact.institute: Vrije Universiteit Medical Center
contact.phone: +31 20 4442428
organism: Mus musculus
description: - oligonucleotides were mapped by using the file MEEBO_track from UCSF
manufacture_protocol: The Oligator "MEEBO" Mouse Genome Set, containing 70-mer oligonucleotides representing 35302 unique genes as produced by Illumina (San Diego, CA, USA). The oligonucleotides were dissolved at 10 μM concentration in 50 mM sodium phosphate buffer pH 8.5 and single spotted onto CodeLink™ slides (Amersham Biosciences), using an OmniGrid® 100 microarrayer (Genomic Solutions, Ann Arbor, MI, USA) equipped with SMP3 pins (TeleChem International, Sunnyvale, CA, USA). After printing slides were processed and blocked prior to use according to the manufacturer’s protocol.