title: Brassica 90K array
status: Public on Dec 30 2014
contact.city: Wuhan
contact.first: Yi
contact.last: Huang
contact.email: huangyi@caas.cn
contact.state: Hubei
contact.address: No. 2 Xudong Second Road, Wuchang District
contact.department: Rapeseed Genetics and Breeding
contact.country: China
contact.institute: Oil Crops Research Institute, Chinese Academy of Agricultural Sciences
contact.phone: +8613886068552
organism: Brassica napus
  Brassica rapa
  Brassica oleracea
manufacture_protocol: Plant Biotechnology Institute, National Research Council Canada (PBI-NRC); A Brassica genomics resource was developed at the Plant Biotechnology Institute, National Research Council Canada (PBI-NRC) and Agriculture and Agri-Food Canada (AAFC) in collaboration with other institutes. About 95,418 unique sequences were assembled using 781,826 EST sequences mainly from three species: B. napus, B. rapa, and B. oleracea. These unique sequences were submitted to CombiMatrix for the development of a 35-40mer oligonucleotide microarray, for which probes were synthesized in situ onto electrodes on a microchip. The customized Combimatrix Brassica array comprised 90,500 probes and was called as 90K array.