title: OSU Streptococcus pyogenes M1 and M18 microarray-SDHHbtail
status: Public on Mar 12 2010
contact.city: Columbus
contact.first: Vijay
contact.last: Pancholi
contact.email: vijay.pancholi@osumc.edu
contact.state: OH
contact.address: 420W 12th Ave, TMRF-288
contact.department: Pathology
contact.country: USA
contact.institute: Ohio State University
contact.phone: 614-688-8053
organism: Streptococcus pyogenes
description: The oligonucleotides were spotted onto poly-L-lysine microarray slides by a robotic microarrayer (Omni GRAD 100 microarrayer) using 12 pin spotter at the Center of Applied Genomics (CAGE) of the Public Health Research Institute, Newark, NJ. Arrays were distributed in 16 blocks each with 12 columns and 12 rows.
  
  
manufacture_protocol: Sense strand oligonucleotides with mainly 55-mer, representing the 1,769 open reading frames (ORFs) in the genome of S. pyogenes Type M1, strain SF370 and 175 ORFs (those absent in SF370 strain) of Type M18 (Strain MGAS8232) were designed and produced by Illumina (http://www.illumina.com). They were obtained as 1.35 nmoles quantity at 50 uM concentration in 27 ul of water in shallow well plates.
  