title: CGH-CHORI-32K-Chr. 12 X Y
status: Public on Sep 18 2007
contact.city: Leuven
contact.first: Rekin's
contact.last: Janky
contact.email: Nucleomics.Bioinformatics@vib.be
contact.address: Herestraat 49 Box 816
contact.department: Nucleomics Core
contact.country: Belgium
contact.institute: VIB
organism: Homo sapiens
description: The array contains 4012 distinct BAC and PAC sequences. The bacs were printed in 2 replicates. In total the array design contains 16092 features printed in a layout of 4 meta rows, 4 meta columns, 23 rows per block and 22 columns per block.This grid is printed twice on the glass slides allowing for two microarray arrays per slide.
manufacture_protocol: MicroGrid Printing ProtocolEquipment:
  1. Microgrid (Genomics Solutions, UK).
  2. 384 microtiter plates (X7022, Genetix)
  3. 4x4 MicroSpot quill pins - 10K (cat# BR001489, Genomics Solutions, UK)
  4. Codelink slides (cat#: 30001100, GE Healthcare).
  Printing 2 arrays of 4x4 blocks on 1 microscope slide:
  1. Dissolve PCR products in 125mM Na-Phosphate  (pH 8.45) buffer, 0.000125% sarcosyl at concentrations of 300 ng/ul.
  
  2. Set spotter settings for 2 arrays of 4x4 blocks printed with 16 pins: column pitch = 185 um, row pitch = 185 um; Humidity 55%; 60 spots per source visit.
  3. Make sure room temperature is between 20-24 degrees Celsius.
  4. Place microtiter plates in the plate hotel with A1 in front left corner.
  5. Place 30 slides on the slide-deck.
  6. Start spotting early in the morning and stop spotting procedure at end of the day and remove pin set. Leave spotter overnight with vacuum and humidity settings on.
  7. Incubate the slides in humid chamber with saturated NaCl for 24 to 72 hours.
  8. Block 1%NH4OH.
  9. Slides can be stored for at least 6 months in a dry-cabinet.