title: University of Alberta Bovine 24K long oligo array
status: Public on Oct 02 2010
contact.city: Tsukuba
contact.first: Masaaki
contact.last: Taniguchi
contact.email: masaakit@affrc.go.jp
contact.state: Ibaraki
contact.address: 2 Ikenodai
contact.department: Division of Animal Breeding and Reproduction Research
contact.country: Japan
contact.institute: Institute of Livestock and Grassland Science National Agriculture Food Research Organization
contact.phone: +81-29-838-8627
organism: Bos taurus
description: UniGene ID of sequence used to design oligonucleotide probe, Gene symbol, GenBank accession number and gene description were annotated in the GeneSifter based on the UniGene ID.
  
  The oligonucleotide sequences are provided as a supplementary file.
manufacture_protocol: 1.Oligos are arrayed in 384-well flat-bottom plates. Each well contains 300 pmol of 70-mer oligo.
  2. Resuspend oligos in 15 µL of 150 mM sodium phosphate buffer (pH 8.5).
  3. Transfer 0.25 µL from each well to to a new set of plate.
  4. Seal plates and centrifuge plates at 2000 rpm for 3 minutes to allow the solution sink to the bottom.
  5. Up take 0.5 nL from each well to spot the oligos onto the UltraGap slides (Corning, USA).
  6. The 70-mer oligonucleotides were spotted at room temperature and 42% humidity with 100 µm centre-spacing using Q-array system with Telechem 946MP2 pins.
  7. The spot diameters were 55 µM with duplicate of 24,000 spots on each slide.
  8. After the print run is completed, allow plates to dry through passive evaporation in a protected environment.
  9. The slides were cross-linked by UV.
  10. The slides were stored at room temperature in dessicators.
  11. After printed the slides, the plates were vacuum dried for storage.