title: BTI_Tomato_12800_v.CGEP
status: Public on Jun 30 2009
contact.city: Québec
contact.first: Jean-François
contact.last: Dubuc
contact.email: jean-francois.dubuc.1@ulaval.ca
contact.address: 2440 boul. Hochelaga
contact.country: Canada
contact.institute: Université Laval
organism: Solanum lycopersicum
description: The array consist of 32 subgrids (4 meta-rows, 8 meta-columns). Each sub-grid contains 20 rows and 20 columns
manufacture_protocol: TOM2 array were available from the Center for Gene Expression Profiling (CGEP) at Boyce Thompson Institute for Plant Research. TOM2 oligo array from tomato contain a total of 12800 elements or approximately 11860 unique elements. Sixty-nine oligonucleotides sequences from unigenes were synthesized by Operon (Operon biotechnologies, Hunstville, AL). Oligos were re-suspended in 12 uL of spotting buffer (3X SSC), and were spotted on Ultra Gaps Coated Slides (Corning, Lowell, MA) using a MicroGrid Pro arrayer (BioRobotics Inc., MA) with 32 MicroSpot2500 printing pins. Temperature and humidity inside the arrayer were maintained at 18-21°C and 35-45% RH, respectively. Oligos were fixed to the slide by 2-h incubation at 80°C.