title: Blanchette/Green Splice Junction array
status: Public on Dec 01 2008
contact.city: Berkeley
contact.first: Kasper
contact.middle: Daniel
contact.last: Hansen
contact.email: khansen@compbio.berkeley.edu
contact.state: CA
contact.address: 461 Koshland Hall
contact.department: PMB
contact.country: USA
contact.institute: UC Berkeley
organism: Drosophila melanogaster
description: This array is a splice junction array designed for D . Melanogaster using FlyBase 4. The array encompasses all genes that are annotated as having alternative transcripts. For each transcript, probes targeting alternative junctions and exons awere designed as well as probes targeting some constitutive (but not all) exons and junctions. The array design is very similar (essentially an update) to the design described in Blanchette et al. "Global analysis of positive and negative pre-mRNA splicing regulators in Drosophila" in Genes Dev 2005.
  Probes on the array were "t-stilted", that is every probe was extended by a poly T-tail in order to move the probe off the array surface, nucleotide sequences are given with and without stilts.
manufacture_protocol: Standard Agilent