title: Human ten thousand 50-mer oligo library from MWGBiotech Oligo sets (http:/www.mwgbiotech.com)
status: Public on Nov 13 2009
contact.city: Mexico City
contact.first: Carmen
contact.last: Sánchez-Torres
contact.email: csanchez@cinvestav.mx
contact.state: D.F.
contact.address: I.P.N. Av. 2508
contact.department: Molecular Biomedicine
contact.country: Mexico
contact.institute: CINVESTAV-IPN
contact.phone: 52 55 5747 3328
organism: Homo sapiens
manufacture_protocol: 1. Oligo sets (http:/www.mwgbiotech.com) were resuspended to 50µM in Micro Spotting solution (ArrayIt Brand Products.).
  2. SuperAmine coated slides 25x75 mm (TeleChem International INC) were printed in duplicate, and fixed at 80 ºC for 4 hours.
  3. For pre-hybridization, slides were re-hydrated with water vapor at 60 ºC, and fixed with two cycles of UV light (1200J). After boiling for two minutes at 92 ºC, slides were washed with 95% ethanol for one minute and prehybridzed in 5X SSC, 0.1% SDS and 1% BSA for one hour at 42 ºC. The slides were washed and dried for further hybridization