title: miRRat_17
status: Public on Mar 25 2012
contact.city: Shanghai
contact.first: Jing
contact.last: Li
contact.address: South Chongqing Road
contact.country: China
contact.institute: Institue of Health Sciences
organism: Rattus norvegicus
manufacture_protocol: Arrays are made based µParaflo microfluidic technology (Atactic Technologies). On the µParaflo microfluidic chip, each detection probe consists of a chemically modified nucleotide coding segment complementary to target microRNA (from miRBase, http://microrna.sanger.ac.uk/sequences/) or other RNA (control or customer defined sequences) and a spacer segment of polyethylene glycol to extend the coding segment away from the substrate. The detection probes are made by in situ synthesis using PGR (photogenerated reagent) chemistry.