title: Ocichip Human 40 K Set (chip A)
status: Public on Nov 01 2010
contact.city: Delhi
contact.first: Vibha
contact.last: Tandon
contact.email: vibhadelhi@hotmail.com
contact.state: Delhi
contact.address: University of Delhi
contact.department: Department of Chemistry
contact.country: India
contact.institute: University of Delhi
organism: Homo sapiens
manufacture_protocol: Human 40 K A Ocichip consisting of 20160 spots and Human 40 K B Consisting of 19968 spots were printed on Corning® epoxy coated glass slides using Omnigrid (Gene Machines). Chip has been printed using 4x12 SMP4 pins on 75.5x 25.5x1.3mm microarray glass slides. The origin offset values were 0 for X direction and 0 for Y direction. The dot to dot distance is 220 um; distance between subarrays is 150um and no of dots per array is 20x21=420. Oligos were printed using spotting buffer A from Ocimum Biosolution at 50% humidity and temperature at 22°C. The time taken by the robotic gridder to print Human 40 K A and B slides altogether was 50 hrs. Each probe was checked for its performance by standard QC process followed at Ocimum Biosolutions. Printed slides were stored in a light-tight box in a bench-top dessicator, with desiccant and were kept at 4°C until they were used for hybridization.