title: M3 (NIA Mouse 15K Clone Set)
status: Public on May 15 2003
contact.city: Memphis
contact.first: Deanna
contact.middle: Cole
contact.last: Naeve
contact.email: deanna.naeve@stjude.org
contact.state: TN
contact.address: 332 N. Lauderdale
contact.department: Hartwell Center for Bioinformatics and Biotechnology
contact.country: USA
contact.institute: St. Jude Children's Research Hospital
contact.phone: 901-495-4847
organism: Mus musculus
description: The M3 microarray includes cDNAs from the NIA Mouse 15K cDNA clone collection.  NIA Mouse 15K is derived from several early embryonic cDNA libraries, including blastocyst, preimplantation, embryonic stem cells, trophoblast stem cells, embryonic germ cells, and others.  The cDNA inserts were amplified from replica plate cultures by PCR, purified and mechanically spotted onto polylysine-coated microscope slides using an OmniGrid robotic spotter (GeneMachines, San Carlo, CA).  Printed slides were post-processed according to the procedures on the Stanford website with some modifications: http://cmgm.stanford.edu/pbrown/protocols/index.html.