title: DKFZ human_naive_T_cells 7_samples v1.0
status: Public on Dec 17 2009
contact.city: Heidelberg
contact.first: Maximilian
contact.last: Aigner
contact.address: Im Neuenheimer Feld 280
contact.country: Germany
contact.institute: Deutsches Krebsforschungszentrum (DKFZ)
organism: Homo sapiens
description: Oligo GEArray 300 Series microarrays can contain up to 276 oligonucleotide spots (including control spots and blanks) which are printed on a 3,8 x 4,8 cm nylon membrane (12 colmns, 23 rows). All oligonucleotide probes are derived from a sequence located at a median distance of approximately 160 nucleotides from the 3’ end of the coding region. Furthermore, gene specificity, sequence complexity, secondary structure, melting temperature and GC content are rigorously evaluated using a SuperArray Bioscience Corporation-proprietary computer algorithm.
  The customized Oligo GEArray COH-0005 described in this platform contains 247 different human gene probes, spotted on 253 positions (i.e. some genes are present in replicates) and 23 "non-human" control positions (e.g. sequences derived from E. coli, artificial sequences, or blanks).
manufacture_protocol: For the Oligo GEArray microarrays the 60-mer oligonucleotide probes are printed on a positively charged, three-dimensional nylon membrane by synQADTM technology (one of the core technologies developed by Genomic Solutions, a Harvard Bioscience company, and marketed under CartesianTM Dispensing Systems). This advanced ink-jet, non-contact dispensing system (10 nL deposition volume only!) is known for its high spotting reproducibility.
  The Oligo GEArrays are designed for one-time use only. Each array membrane (containing a unique bar-coded serial number on its reverse side) is packaged, stored and used in its own disposable hybridization tube.