title: 01_Drosophila_16K_Universitat_Barcelona
status: Public on Oct 23 2006
contact.city: Barcelona
contact.first: Sergi
contact.last: Beltran
contact.address: Baldiri Reixac 10
contact.department: Serveis Cientificotècnics
contact.country: Spain
contact.institute: Universitat de Barcelona
organism: Drosophila melanogaster
description: Oligonucleotide Drosophila microarray platform containing virtually all genes from the genome. In addition to the Operon Drosophila Genome Oligo Set Version 1.1, a number of positive and negative controls have been printed so that quality of hybridizations and analysis procedures can be assessed.
manufacture_protocol: Microarrays were printed on Corning UltraGAPS slides with a Genetix QArray using a 16 pin head, yielding a microarray with 48 Blocks and 16416 spots. The oligonucleotides printed are from the Drosophila Genome Oligo Set Version 1.1 (Operon Biotechnologies, Inc), a collection of 14593 probes representing 13577 Drosophila genes with Flybase ID. Additionaly, ten Arabidopsis thaliana 70mer sequences from TIGR (Wang et al., 2003) and spots with no material or with buffer were also printed to be used as spike-in and negative controls, respectively. Spike-in controls were printed on each block (total of 640 spots) so that print-tip and spatial variation along the microarray can be assessed. Twelve Drosophila genes have been printed 17 times to assess reproducibility of hybridization. All oligonucleotides were ressuspended in Pronto! Universal Spotting Solution (Corning).