title: JCVI Pseudomonas fluorescens Pf-5 28k v2.0
status: Public on Sep 01 2009
contact.city: Sydney
contact.first: Ian
contact.last: Paulsen
contact.email: ipaulsen@cbms.mq.edu.au
contact.state: NSW
contact.address: Macquarie University
contact.department: Department of Chemistry and Biomolecular Sciences
contact.country: Australia
contact.institute: Macquarie University
organism: Pseudomonas protegens Pf-5
manufacture_protocol: Unique 70-mer oligonucleotides were designed for 6147 genes of the Pseudomonas fluorescens Pf-5 genome. Oligonucleotides were suspended in 50% DMSO and spotted onto Corning UltraGAPS slides using an Intelligent Automation Systems arrayer. Each gene is represented four times on the array. Negative controls include 50% DMSO / 50% DI water and air. Arabidopsis spike-in controls and Arabidopsis 70-mer oligonucleotides were used as positive controls. Slides were air dried for at least 30 minutes after printing. DNA was irreversibly cross-linked to the slides with 250 mJ/cm^2 UV energy by using a Stratagene Stratalinker. Slides were stored at room temperature in a dessicator.