title: China CDC Shigella flexneri 4224
status: Public on Mar 21 2012
contact.city: Beijing
contact.first: Hua
contact.last: Fu
contact.address: 6#, Rongjing East Street
contact.country: China
contact.institute: Institute of pathogen biology
organism: Shigella flexneri
manufacture_protocol: The S. flexneri whole genome microarrays were designed by using our sequenced strain and were constructed in accordance with previous reports[5,11]. Generally, Sf301 ORFs were amplified with ORF-specific primer pairs. Amplified products, which mostly ranged between 300 and 700 bases in length, were purified by using MultiScreen-PCR plates (Millipore). Concentrations of PCR products were measured by Spectra MAX 190. Then, the PCR products were readjusted to 100 ng/μl of concentration with deionized water and double diluted into spotting solution containing 60% DMSO. The PCR products were spotted onto gamma amino propylsilan-coated GAPII slides (Corning) by using Omni-Grid™ microarrayer (GeneMachines).