title: Genopal Human 216 Autoimmunity Array v.1
status: Public on Nov 30 2009
contact.city: Suita
contact.first: Daisuke
contact.last: Okuzaki
contact.email: dokuzaki@biken.osaka-u.ac.jp
contact.state: Osaka
contact.address: Yamadaoka 3-1
contact.department: Immunology Frontier Research Center
contact.country: Japan
contact.institute: Osaka univ.
contact.phone: +81-6-6879-4935
organism: Homo sapiens
manufacture_protocol: Custom-made fibrous oligonucleotide DNA microarray GenopalTM (Mitsubishi Rayon) with a total of 216 genes which were selected from autoimmune disease related genes.
  
  All oligonucleotide capture probes (65 mer in length), including those for negative controls, were designed to have approximately the same annealing temperature (Tm) 　throughout a microarray. In addition, they were designed also to hybridize to the target antisense RNA specifically with a domain within 1 kb from 3’-end.
  
  The oligonucleotide DNA microarray, GenopalTM (Mitsubishi Rayon), was made in the following manner. Plastic hollow fibers, in which oligonucleotide capture probes were chemically bonded to hydrophilic gel, were bundled in an orderly arrangement, hardened with resin to form a block (12 rows × 19 columns) and then sliced into thin chips. Finally, three thin chips were set into a holder together.