title: Agilent custom 244K oligo RNA Binding Protein microarray v2
status: Public on Jun 07 2009
contact.city: San Francisco
contact.first: Esther
contact.middle: T
contact.last: Chan
contact.state: California
contact.address: 1500 Owens Street, Suite 500
contact.country: USA
contact.institute: Celgene Corporation
organism: synthetic construct
description: This custom microarray consists of probes representing two complete sets of (1) all possible 10-base sequences (contained within longer unstrctured RNAs) and (2) all possible 7- and 8-base loop sequences in the context of RNA hairpins containing unique 10 base pair stems. An RNA pool is synthesized from on the array and subsequently stripped from the array and PCR-amplified to create a dsDNA pool. This pool is then used as a template for in vitro T7 RNA polymerase-mediated RNA transcription to generate an RNA pool used for pull-down experiments using GST-tagged RNA binding proteins. The RNAs pulled-down by each RNA-binding protein are then labelled with a fluorophore and hybridized back onto the array, along with the total pool.
manufacture_protocol: Please refer to the manufacturer's web site at http://www.agilent.com/