title: MRC Toxicology Unit, Mus musculus, oligo MEEBO microarrays
status: Public on Jun 01 2010
contact.city: Leicester
contact.first: Amy
contact.last: Pointon
contact.address: Lancaster Road
contact.department: Systems Toxicology Group
contact.country: United Kingdom
contact.institute: MRC Toxicology Unit
organism: Mus musculus
manufacture_protocol: 1.Oligos are in 101 384 well plates (MEEBO set) (Cat OL -10-122).  Each well contains 200 pmol of 70-mer oligo.  Print split over 2 slides – A and B.
  2. Resuspend oligos in 20ul printing buffer to 10uM.  Printing buffer is 1.5M betaine, 3xSSC.
  3. Seal plates with adhesive PCR film from Applied Biosystems (cat. AB-0558).
  4. Shake plates on a multi plate shaker for about 15 minutes This resuspends the dried down oligo in the printing buffer.
  5. Centrifuge plates at 3000rpm for 5 minutes.
  6. Remove seal immediately prior to use. Place one plate at a time in appropriate location of plate holder on arrayer.  Screw to keep in position.  Attach 32 pin head to standford design arrayer and start print run.  After all 384 samples have been printed software will request next plate be placed in position.  Remove previous plate, reseal and replace with next plate.