title: Trichoderma array (1.5 K) 'est_unig1'
status: Public on Aug 22 2006
contact.city: Irapuato
contact.first: Enrique
contact.middle: Ibarra
contact.last: Laclette
contact.email: eibarra@ira.cinvestav.mx
contact.state: Guanajuato
contact.address: Km 9.6 Libramiento Norte Carretera Irapuato-León, A.P. 629
contact.country: Mexico
contact.institute: Laboratorio Nacional de Genomíca (LANGEBIO), Centro de Investigación y Estudios Avanzados del IPN (CINVESTAV)
contact.phone: (462) 166 3000
organism: Trichoderma atroviride
manufacture_protocol: Custom cDNA-based Microarray: DNA probes to a set of 1,655 strategically selected genes were generated by PCR. The DNA concentration was determined using Nanodrop equipment. Samples were transferred to 96 well plates containing DMSO to give a final concentration of 200 ng/ul in 50% DMSO. The DNA probes were directly spotted onto Corning UltraGAPS coated glass slides. Each of the genes selected was printed in two contiguous replicates laid out in 8 grids of 22 rows by 22 columns.