title: BRAF/MEK inhibitor resistance melanoma cells treated with PAK inhibitor PF3758309 array
status: Public on Jul 31 2017
contact.city: Toronto
contact.first: Hezhe
contact.last: Lu
contact.email: lvhezhe@gmail.com
contact.state: Ontario
contact.address: 600 University Ave
contact.country: Canada
contact.institute: The Lunenfeld-Tanenbaum Research Institute
organism: Homo sapiens
manufacture_protocol: We stained slides for more than 200 unique antibodies and 3 secondary antibody negative controls which were analyzed on Array-Pro then by supercurve Rx64 3.1.1. There were 12 sets of replicated antibodies among slides. We performed QC test for each antibody staining (slide). The QC Score (Probability) values were also included in the data spreadsheet for your references. QC score above 0.8 indicates good antibody staining. We only included in the data for the all individual antibodies with QC Scores higher than 0.80 in the Heatmaps.  In the case of antibodies with replicates, the one with the highest QC Score was used.  For samples of tissue origin, we removed 7 antibodies cross react with damaged components (with unknown mechanism). There are 51 individual mouse antibodies (labeled -M) that will be removed for mouse tissue or xenograft samples, unless you specifically indicated for inclusion in your sample submission form.