title: FUGE Hs-OperonV3
status: Public on Aug 18 2006
contact.city: Oslo
contact.first: Bjorn
contact.last: Grinde
contact.email: bjgr@fhi.no
contact.state: Oslo
contact.address: Geitmyrsveien 75
contact.department: Infectious Disease Control
contact.country: Norway
contact.institute: Norwegian Institute of Public Health
contact.phone: +47 22042420
organism: Homo sapiens
manufacture_protocol: Array Production Details:
  •Spotting Instrumentation: MicroGrid II arrayer (model# 600),
  MicroSpot pin tool (Cat# BR001482),
  MicroSpot 10 K Split pins (Cat# BR001489),
  BioRobotics (Genomic Solutions, http://www.genomicsolutions.com//files/MicroGrid.pdf )
  •Slide substrates: CMT UltraGAPS slides (25x75 mm) , Aminosilane coating (Corning Cat# 40016)
  •Oligonucleotide consentration: 40 μM (600 pmol oligonucleotide dissolved in 15 μL 50% DMSO)
  •Spotting buffer: 50% Dimethyl Sulphoxide (DMSO), (Sigma Cat# D-8418)
  •Printing plates: 384 well low profile plates from Genetix (Cat# X60004)
  •Spotting conditions: 20–22°C and at 45–55% RH.
  •Slide post-processing: Slides are stored in a desicator cabinet to dry for 24-48 hours after spotting, and UV-crosslinked at 800 mJ/cm2
  (active side up) using a UV-Stratalinker 2400 from Stratagene Inc. The arrays are stored in Corning slide boxes before use.