title: Agilent 22K custom mouse gene expression array 1 of 2
status: Public on Apr 18 2007
contact.city: Toronto
contact.first: Emily
contact.last: Walker
contact.email: emily.walker@utoronto.ca
contact.state: ON
contact.address: 164 College Street Room 407
contact.department: Institute for Biomaterials and Biomedical Engineering
contact.country: Canada
contact.institute: University of Toronto
organism: Mus musculus
manufacture_protocol: Hydrophobic glass wafers (75 × 75 mm) containing exposed hydroxyl groups for nucleotide coupling were prepared using a selfassembling silane mixture to form a uniform, hydrophobic surface with a controlled density of hydroxyl groups extending by a single methylene unit above unfunctionalized silane components. Phosphoramidite monomers in propylene carbonate were delivered to defined positions on the glass surfaces using commercial ink-jet printer heads. Synthesis cycles were otherwise similar to traditional oligonucleotide synthesis, with the exception that capping was omitted. After manufacture, arrays were diced to 25 × 75 mm to accommodate commercial scanners.