title: Affymetrix Escherichia coli full sequence array (EcFS) [EcFS_1, EcFS_2, and EcFS_3]
status: Public on Mar 29 2011
contact.city: Tsukuba
contact.first: Bei-Wen
contact.last: Ying
contact.email: ying.beiwen.gf@u.tsukuba.ac.jp
contact.state: Ibaraki
contact.address: 1-1-1 Tennoudai
contact.country: Japan
contact.institute: University of Tsukuba
contact.phone: 81 29 853 6633
organism: Escherichia coli
description: A high-density DNA microarray (Ono et al, 2008; Suzuki et al, 2007) covering the whole genome of E. coli W3110 strain (GenoBase, http://ecoli.naist.jp/GB6/search.jsp) was utilised. Both strands of the genome were alternately tiled at single-base resolution using 21-mer perfectly matching and three mismatching probes. A library consisting of a total of 18.6 million probes was arranged on three GeneChips for microarray analysis, covering four types of probe throughout the 4.6-Mb E. coli genome. One of three GeneChips was used here for transcriptional analysis.
manufacture_protocol: http://www.affymetrix.com/estore/