title: CapitalBio Mammalian miRNA Array Services V1[1].0
status: Public on Dec 31 2008
contact.city: Shanghai
contact.first: JIANREN
contact.last: GU
contact.email: nlorg@sh163.net
contact.address: No.25/Ln.2200, Xietu Road,
contact.department: State Key Laboratory of Oncogenes and Related Genes
contact.country: China
contact.institute: Shanghai Cancer Institute, Shanghai Jiao Tong University
contact.phone: 86-21-64177401
organism: Homo sapiens
manufacture_protocol: The limited sequence length of miRNAs leaves little consideration for probe design strategy; all miRNA probe sequences were designed to be complementary to the full-length mature miRNA. To facilitate probe immobilization on aldelhyde modified glass (CapitalBio Corp., Beijing, China), the probe sequences were concatenated up to 40 nt and modified with 5′-amino-modifier C6 oligonucleotide. In addition, we designed 8 short oligos with no homology to existing RNA sequences to serve as negative controls; the corresponding synthetic miRNAs were generated by in vitro transcription using Ambion's miRNA Probe Construction Kit (Ambion, Austin, TX). Various amounts of synthetic miRNAs were spiked into the interrogated RNA samples.