title: NCI/LMT Mm_FCRF_CGEN oligo
status: Public on Oct 27 2004
contact.city: Frederick
contact.first: Lisa
contact.middle: M
contact.last: Gangi
contact.email: gangil@pop.nci.nih.gov
contact.state: MD
contact.address: 915 Tollhouse Ave
contact.department: LMT-Frederick
contact.country: USA
contact.institute: NCI
contact.phone: 301-846-6296
organism: Mus musculus
description: Printing by the NCI-Frederick/LMT Microarray Research Facility
  Surface type: Surface type: amino-silane coated glass Corning CMT-GAPS II (http://www.corning.com)
  Attachment: covalent, (UV-treated)
  Array description: The custom arrays are manufactured by the NCI Frederick Laboratory of Molecular Technology Microarray Facility (NIH/NCI/SAIC/LMT).  Microarrays are printed with BioRobotics TAS II robotic arrayer from Genomic Solutions (http://www.genomicsolutions.com) that is fitted with a printhead containing 32 SMP3 printing pins from Telechem International (http://www.arrayit.com).  The mouse microarray (Mm_FCRF_CGEN oligo) probe set is based on 22,271 65mer oligonucleotides from Compugen Mouse Genome Oligo Set V1.0 (http://www.labonweb.com). The set includes 17,815 Unique UniGene Clusters that mapped into build #120. Oligonucleotide probes are distributed into 384-well polypropylene plates (Genetix, 384 micro array) and dissolved in 3XSSC print buffer at a normalized 25 micromolar concentration. The Mm_FCRF_CGEN oligo microarray spot densities are 165 microns (center-to-center) and one spot is printed per probe per slide.  The microarrays are printed on Corning CMT-GAPS II slides (Item #40004) coated with amino-silane and post-processed according to the manufacturer's protocol.