title: AFGC 15K cDNA array
status: Public on Apr 15 2013
contact.city: Taipei
contact.first: Cheng-En
contact.last: Chen
contact.email: f93633004@ntu.edu.tw
contact.address: No.1, Sec.4, Roosevelt Road.
contact.country: Taiwan
contact.institute: National Taiwan University
organism: Arabidopsis thaliana
manufacture_protocol: The DNA elements for the DNA microarrays were amplified as part of the Arabidopsis Functional Genomics Consortium (AFGC). The DNA solutions were printed on CMT-GAPS2-coated glass slides (Corning, http://www.corning.com/) with use of the OmniGrid 100 microarrayer (GeneMachines; http://www.genomicsolutions.com). After printing, slides were baked at 80 C for 6 h and blocked (0.1 mg ml)1 BSA, 3· SSC, 0.1% SDS) before hybridization.