title: UMDNJ/Lukac Human herpesvirus 8/107+GAPDH
status: Public on Aug 30 2008
contact.city: Newark
contact.first: David
contact.middle: M.
contact.last: Lukac
contact.email: lukacdm@njms.rutgers.edu
contact.state: NJ
contact.address: 225 Warren St.; ICPH E350C; PO Box 1709
contact.department: Microbiology, Biochemistry and Molecular Genetics
contact.country: USA
contact.institute: Rutgers Univ./NJ Medical School
contact.phone: 973 972-4868
organism: Human gammaherpesvirus 8
manufacture_protocol: The KSHV microarray consists of 60-70-mer oligonucleotides (oligos) designed to detect 96 predicted KSHV ORFs and splice variants, identical to those published by Lu, et al (Journal of Virology, December 2004, p. 13637-13652, Vol. 78, No. 24), updated to include newer annotated transcripts.  A detector oligo for the Human housekeeping genes glyceraldehyde-3-phosphate dehydrogenase was also synthesized. Operon Biotechnologies synthesized all oligos with 5' amino-C6 modifications. Oligos were spotted in triplicate onto poly-L-lysine-coated glass microscope slides using a GeneMachines Omnigrid 100 Arrayer (Genomic Solutions) and SMP3 pins (Telechem).