title: Hsbb-20
status: Public on May 14 2009
contact.city: Bethesda
contact.first: Sasisekhar
contact.last: Bennuru
contact.email: bennurus@niaid.nih.gov
contact.state: MD
contact.address: 10 Center Dr, Bldg 10, Rm 11N208
contact.department: LPD
contact.country: USA
contact.institute: NIH/NIAID
contact.phone: 301-761-5411
organism: Homo sapiens
description: The custom microarrays are manufactured by the Microarray Research Facility of the National Institute of Allergy and Infectious Diseases (NIH/NIAID). Microarrays are printed with an Omnigrid robotic arrayer by Genomic Solutions (http://www.genomicsolutions.com ) that is fitted with a printhead and 48 SMP3 print tips from Telechem (http://www.arrayit.com ). The human microarray (Hsbb) probe set is based on 70mer oligonucleotides from Qiagen's Human Genome Oligo Set V2.0 ( http://oligos.qiagen.com/arrays/omad.php ). A comprehensive gene list is also available at http://madb.niaid.nih.gov/
manufacture_protocol: Oligonucleotide probes are distributed into 384-well polypropylene plates (Genetix, 384 micro array) and dissolved to a normalized 25 micromolar concentration in print buffer (150mM sodium phosphate, pH 8.5, 4% sodium sulfate, 0.0001% N-lauryl sarcosyl). The Hsbb microarray spot densities are 190 microns (center-to-center). The microarrays are printed on MWG Epoxy Slides (MWG Biotech Inc., # 1150-000000) and treated by a method that is an adaptation of the manufacturer's instructions to allow higher throughput processing.