title: LAM Subarray 63 - Antigens 10/22/2017
status: Public on Jul 21 2021
contact.city: Bronx
contact.first: Jacqueline
contact.last: Achkar
contact.email: jacqueline.achkar@einsteinmed.org
contact.address: 1300 Morris Park Ave.
contact.country: USA
contact.institute: Albert Einstein College of Medicine
contact.phone: 7184302971
organism: synthetic construct
description: NA - Synthetic antigens coupled to bovine serum albumin
manufacture_protocol: Microarray slides were printed at Engineering Arts LLC (Phoenix, AZ). BSA conjugates were noncontact printed on Schott type E slides (Schott North America, Inc., Elmsford, NY) using an Engineering Arts au301 Rainmaker microarray printer and dispensed at 100 μg/mL in buffer (1:10 phosphate-buffered saline plus 0.005% vol/vol Triton X-100). Antigens were dispensed at 360 pL per spot to produce 140- to 160-m diameter spots in triplicate. Relative humidity was maintained at 65%; slides were subjected to white light for 100% inline drop inspection. Printing was carried out in a HEPA-filtered environment at room temperature. Printed slides were stored at –20 °C until use. Each slide contains 24 identical array containing 30 components printed in duplicate.