title: Lactococcus lactis subsp. lactis IL1403 4.2K Eurogentec microarray
status: Public on Mar 22 2010
contact.city: Belfast
contact.first: Guillermo
contact.last: López-Campos
contact.state: Northern Ireland
contact.address: 97 Lisburn road
contact.department: Centre for Experimental Medicine
contact.country: United Kingdom
contact.institute: Queen's University of Belfast
organism: Lactococcus lactis subsp. lactis Il1403
manufacture_protocol: Amplicons were designed using GenomePrimer in combination with UniFrag, amplified by Eurogentec (Belgium), purified using Qiagen 96-wells purifying plates. Amplicons (about 10 microL) diluted 2x with ArrayIT 2x spotting buffer, in Genetix 384 wells spot plates, spotted on aldehyde slides (ArrayIt) with a GenPak spotter at about 55 % humidity. Blocking of reactive aldehyde groups by boric anhydride treatment.