title: YALE/NIMBLEGEN HUMAN-CHR11-beta-globin-locus 3K v1.0
status: Public on Feb 16 2006
contact.city: New Haven
contact.first: Jan
contact.middle: Oliver
contact.last: Korbel
contact.state: CT
contact.address: 266 Whitney Avenue
contact.country: USA
contact.web_link: http://bioinfo.mbb.yale.edu/array/
contact.institute: Yale University
contact.phone: +1 203 432 5405
organism: Homo sapiens
manufacture_protocol: Maskless array synthesis [1] was used to construct isothermal arrays, This array contains oligonucleotides that start every 9 bp on average and tiles the beta-globin locus on chromosome 11 (human genome build NCBI35/hg17 was utilized). The oligonucleotides were designed to be of variable length, adjusted to match a target melting temperature of 76°C according to the following formula: Tm = 81.5 + 16.6*(log10([Na+])) + 0.41*(%GC) - 600/length. Probe lengths were constrained to be a minimum of 45 bp and maximum of 85 bp.
  
  [1] Nuwaysir, E.F., Huang, W., Albert, T. J., Singh, J., Nuwaysir, K., Pitas, A., Richmond, T., Gorski, T., Berg, J. P., Ballin, J. et al., Genome Res., 2002. 12: p. 1749-1755, PMID: 12421762, http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=search&term=12421762