title: IQ-USP Gracilaria tenuistipitata 4.6K v1.0
status: Public on Jun 01 2013
contact.city: São Paulo
contact.first: Paulo
contact.middle: A.
contact.last: Zaini
contact.email: paulo.zaini@gmail.com
contact.state: São Paulo
contact.address: Av. Prof. Lineu Prestes, 748 - Butantã
contact.department: Bioquímica
contact.country: Brazil
contact.institute: Universidade de São Paulo
organism: Gracilaria tenuistipitata
manufacture_protocol: A 4,608-element DNA microarray was printed containing unique internal fragments of 1,448 CDS spotted at least in duplicate. DNA fragments ranging from 200 to 800 bp were PCR amplified from an EST library. PCR products in 50% DMSO were spotted with a Generation III Spotter (GE Healthcare, Little Chalfont, England) and kept in low humidity (<15%) until use.