title: Ocimum Biosolutions Arabidopsis 25K OciChip
status: Public on Feb 24 2010
contact.city: Hyderabad
contact.first: Ocimum
contact.last: Biosolutions
contact.email: govind.a@ocimumbio.com, sandhya.p@ocimumbio.com, hazira.b@ocimumbio.com, anwaruddin.m@ocimumbio.com
contact.state: Andhra Pradesh
contact.address: Banjara Hills Road No., 1 VI Floor Reliance Classic
contact.department: Bioinformatics
contact.country: India
contact.institute: Ocimum Biosolutions
organism: Arabidopsis thaliana
manufacture_protocol: Array Design and Printing
  Arabidopsis 25K Ocichip consisting of 24960 spots printed on Corning® epoxy coated  glass slides using Omnigrid (Gene Machines). Chip has been printed using 4x12 SMP3 pins on 75.5x 25.5x1.3 mm microarray glass slides. The origin offset values were 0 for X direction and 0 for Y direction. The dot to dot distance is 180 mm; distance between subarrays is 150 mm and no of dots per array is 23x23=529. Oligos were printed using spotting buffer A from Ocimum Biosolution at 50% humidity and temperature at 22°C. The time taken by the robotic gridder to print Arabidopsis 25K altogether was 30 hrs. Each probe was checked for its performance by standard QC process followed at Ocimum Biosolutions. Printed slides were stored in a light-tight box in a bench-top dessicator, with desiccant and were kept at 4°C until they were used for hybridization.