title: Synechocystis sp. PCC 6803 oligo array
status: Public on Jul 24 2008
contact.city: St. Paul
contact.first: Zhigang
contact.last: Zhang
contact.state: MN
contact.address: 1479 Gortner Avenue, Suite 140
contact.department: BioTechnology Institute
contact.country: USA
contact.institute: University of Minnesota
organism: Synechocystis sp. PCC 6803
manufacture_protocol: 1. The 70-nt oligomers were designed using ArrayOligoSelector [http://arrayoligosel.sourceforge.net/] and were synthesized by Invitrogen Corp. (Carlsbad, CA).
  2. The oligos were resuspended in 5µl of 3×SSC in 384-well microwell plates to a final concentration of 66.7 pmol/µl and spotted onto poly-L-lysine coated microscopic glass slides using a 16-tip OmniGrid Microarray printer (GeneMachines, San Carlos, CA) as described previously (Eisen 1999).