title: Incyte Drosophila LifeArray v1.0
status: Public on Nov 14 2001
contact.city: Bethesda
contact.first: Brian
contact.last: Oliver
contact.email: briano@nih.gov
contact.state: MD
contact.address: 50 South Drive
contact.department: LBG
contact.country: USA
contact.institute: NIDDK, NIH
contact.phone: 301-204-9463
organism: Drosophila melanogaster
description: The array was assembled using amplicons representing approximately 93% of the genes predicted in version 1.0 of the Drosophila genome from sequences released by the Berkeley Drosophila Genome Project and Celera Genomics in March 2000.  The amplicons represent approximately 75% of the genes predicted in the January 2003 release 3.1 Drosophila genome annotation update.
  
  Incyte in-house algorithms were used to develop gene-specific primers averaging 20 nucleotides in length that were used to PCR amplify one exon or exon fragment from every predicted gene in the fly genome. The regions amplified to build the array ranged in size from 150 to 600 bp with an average size of 410 bp.  PCR fragments were amplified and each amplicon was quality control tested for DNA concentration, purity, and fragment size.  A total of 13,856 unique Drosophila elements are included on the array.  Each element was spotted in duplicate(some are spotted multiple times) for a total of 29,222 elements which were distributed on two separate glass slides designated subarrays A and B.  In addition to the Drosophila elements, both halves of the array contain non-Drosophila controls elements that serve to orient the arrays, function to correct for background and to provide calibration standards.  The total number of elements (Drosophila transcripts and controls) is 31,464.  Each array element includes an area of 150 micron diameter with a center to center separation of 170 microns.  Adhesion to glass was facilitated by UV crosslinking at 120,000 mj/cm2.
  Qualification of the arrays was examined by dying representatives of each print run with Syto 61 dye. Dyed slides were read with a GenePix 4000A scanner (Axon Instruments, Foster City, CA) at 535 nm.
  The April 2007 platform update is based on the Dmel_RELEASE 4.1 re-annotation result from DGRC (https://dgrc.cgb.indiana.edu/microarrays/annotation.html).