title: AGRF Leptospira interrogans spotted oligo array
status: Public on Mar 22 2010
contact.city: Clayton
contact.first: Miranda
contact.last: Lo
contact.address: Wellington Road
contact.country: Australia
contact.institute: Monash University
organism: Leptospira interrogans
manufacture_protocol: 1. Oligos were transferred to 384 well printing plates and resuspended at 250ng/ul in 3xSSC buffer for printing.
  2. The prepared oligos were arrayed onto Schott A+ aminosilane glass slides using the ESI Virtek Arraying Robot. DNA was spotted using Point Technology split pins.
  3. Slides were baked and UV irradiated at 700MJ to crosslink the DNA.