title: HUMAN_21K_OligoArray_2

status: Public on May 14 2004

city: San Antonio

first: Phillip

last: Bowman

state: TX

address: 3400 Rawley E. Chambers Ave

institute: Institute of Surgical Research

description:
Spotted 70mer oligonucleotide.
The Human Array Ready Oligo Set Version 2 contains arrayable 70mers representing 21,329 well-characterized human genes from the UniGene Database. This database is located at the National Center for Biotechnology Information (see www.ncbi.nlm.nih.gov/UniGene/). All 70mer oligonucleotides in the Human Genome Oligo Set Version 2 were designed after the representative sequences in the UniGene Database, Build Hs 147.
The 70mers have also been Tm normalized to 78°C (±5 C). The Tm normalization allows for a more stringent hybridization at higher temperatures. When a 70-mer could not be designed within the specified Tm range, a shorter or longer length oligo was substituted to maintain the stringent Tm conditions. There are also 12 positive controls and 12 negative controls provided in 16 replicates.

middle: D

first: Baiteng

last: Zhao


title: RNG-MRC_HU2k_v1

status: Public on Sep 23 2004

city: Valbonne - Sophia-Antipolis

first: Kevin

last: Lebrigand

address: 660 route des lucioles

country: France

institute: IPMC/CNRS

organism: Homo sapiens

technology: spotted DNA/cDNA

distribution: custom-commercial

first: Kévin

last: Le Brigand

first: Pascal

last: Barbry


title: yeast12corning

status: Public on Feb 16 2004

city: Madrid

first: Javier

last: Arroyo

state: Madrid

address: Plaza Ramon y Cajal S/N

department: Microbiologia II

country: Spain

institute: Facultad de Farmacia (UCM)

organism: Saccharomyces cerevisiae

description:
12 subarrays (spots organised in 24 rows and 24 columns per subarray)
distributed in 6 rows and 2 columns of subarrays. Controls are ycxA, yceG, ybdO, ybdO, ybaS, ybaF, yacK, ybbR, ybaC, yabQ from B.subtillis, FSV and empty spots.

first: Raúl

last: García

first: Clara

last: Bermejo

first: Cecilia

last: Grau

first: Rosa

last: Pérez

first: Jose

last: Rodríguez-Peña

first: Jean

last: Francois

first: César

last: Nombela


title: Yeast genes

status: Public on Sep 07 2004

city: Paris

first: Gaëlle

last: Lelandais

address: 46, rue d'Ulm

department: Biologie

institute: Ecole Normale Supérieure

description: Time-course analyses of the modifications of the yeast gene expression program which immediately follows addition of the antimitotic drug benomyl

distribution: non-commercial

first: Anca

last: Lucau-Danila

first: Zuzana

last: Kozovska

first: Véronique

last: Tanty

first: Thierry

last: Delaveau

first: Frédéric

last: Devaux

first: Claude

last: Jacq


title: RNG-MRC_HU2k_v2

first: Kevin

last: Lebrigand

first: Kévin

last: Le Brigand


title: Mouse Skeletal Muscle Array

status: Public on Sep 30 2004

city: Bern

first: Christoph

last: Däpp

department: Functional Anatomy

country: Switzerland

institute: University of Bern

organism: Mus musculus

description: Nylon, custom-designed murine cDNA array produced by Clontech, BD Biosciences. The cDNA fragments (200-600 bp) are PCR-amplified using gene-specific primers, and are selected so that they lack repetitive elements and have minimal homology with other genes on the array.

first: Martin

last: Flück

first: Hans

last: Hoppeler


title: microarray core facility of Nantes - West genopole

status: Public on Jul 02 2004

city: Nantes

first: Nolwenn

last: Le Meur

address: 1 rue Gaston Veil

institute: INSERM

technology: spotted oligonucleotide

description:
The array is constituted of 4217 oligos or pcr-products, representing 3525 genes, spotted in quadruplicate. These genes are striated muscle specific (skeletal and cardiac), identified thanks to nine different theme. This multi-dedicated chip is called the “Myochips”.
Keywords = Myochip


title: Chick Pineal 2004

status: Public on Jul 15 2004

city: College Station

first: Phillip

middle: D.

last: Beremand

department: Biology

country: USA

institute: Texas A&M University

organism: Gallus gallus

description:
The cDNA microarrays were prepared based upon approximately 4,500 PCR products from each of the two chicken pineal cDNA libraries constructed, for a total of ~9,000 ESTs. Briefly, the cDNA in plasmids from each library were PCR amplified using flanking primers (SK and T7), purified by ethanol precipitation using ammonium acetate, and placed in the wells of 96 well plates at a concentration of 50 ug/ml in 3X SSC. A GeneMachines OmniGrid microarrayer equipped with 8 Telechem SMP3 pins was used to spot the samples onto poly-L-lysine coated slides (CEL Associates). Duplicate 100 micron diameter spots were placed at intervals of 190 microns (center to center). Slides were printed in batches of 50–100. Printing was accomplished at 70 C and 60% humidity. The OmniGrid’s operating software used information on the position of the individual
clones within the 96-well sample plates and the order in which the plates were used to create a file describing the position of each cDNA clone on the array for later analysis.
Slides were stored desiccated at room temperature until their subsequent use. Prior to hybridization, the dried spots were hydrated gently over a steaming water bath, snap dried, and the DNA was UV cross-linked using a Stratalinker (Stratagene).
Keywords = chick pineal gland
Keywords = circadian oscillator

first: Michael

middle: J

last: Bailey

first: Rick

last: Hammer

first: Elizabeth

last: Reidel

first: Terry

middle: L

last: Thomas

first: Vincent

middle: M

last: Cassone


title: IRIBHM

status: Public on Feb 27 2004

city: Brussels

first: Jérôme

last: Becker

country: Belgium

institute: IRIBHM

description: cDNA clones were collected from resting and stimulated leukocyte cDNA libraries (ResGen Invitrogen Corporation).

first: Jérôme AJ

first: Marie-Jeanne

last: Simons

first: Gilbert

last: Vassart

first: Marc

last: Parmentier

first: Frédérick

last: Libert


title: Incyte Human UniGEM V 2.0 (GEM-5112)

status: Public on Mar 05 2004

city: Chicago

first: Luting

last: Xu

state: IL

address: 303 E. Chicago Ave.

department: Rheumatology Division

institute: Northwestern University Feinberg School of Medicine

description:
The Incyte Human UniGEM V 2.0 (GEM-5112) gene expression microarray contained 9,182 elements/8,556 unique human cDNA genes and ESTs. The clones are derived from Incyte’s set of sequence-verified cDNA clones and represent unique human genes identified in NCBI’s UniGene database. For more information see: Agilent Technologies Human 1 cDNA Array Gene List http://www.chem.agilent.com/cag/bsp/cDNAGL/human1_clonesetB2.htm
Keywords = high density cDNA microarray

first: Li

last: Zhang

first: Yajun

last: Yi

first: Hee-Kap

last: Kang

first: Syamal

middle: K

last: Datta


title: BMAP_HGPD

status: Public on Oct 13 2004

city: Winnipeg

first: Garrett

last: Sorensen

country: Canada

institute: Canadian Science Centre for Human and Animal Health

description: Libraries and clones derived from brain regions of adult mouse (strain c57BL6) were obtained from Research Genetics, Inc. The 11,136 clones represent expressed sequence tags (ESTs) from some 5,471 unique known genes and 5,665 mRNAs as yet uncharacterised, and form part of a library created as part of the Brain Molecular Anatomy Project (BMAP). Each insert from this BMAP 3 EST library was amplified by PCR. The cDNA was purified using Millipore multiwell purification plates (Millipore Inc.), and lyophilised PCR products were resuspended in 1 X Micro Spotting Solution Plus (TeleChem International, Inc.) at a concentration of 0.25-0.75 µg/µl. DNA was spotted onto CMT-GAPS coated glass slides (Corning) using Stealth micro-spotting pins (TeleChem International, Inc.).

first: S

last: Booth

first: Christopher

last: Bowman

first: Richard

last: Baumgartner

first: G

first: C

last: Robertson

first: M

last: Coulthart

last: Phillipson

first: Rajmund

last: Somorjai


title: VIB Mouse 5K 5

status: Public on Nov 25 2003

city: Leuven

first: Rekin's

last: Janky

address: Herestraat 49 Box 816

department: Nucleomics Core

institute: VIB

description:
[1]. Array design:
This microarray contains 4608 array elements spotted in duplicate. Since the microarray spotter is equipped with 12 capillary pins, the spots are arrayed over the entire length of the slide in 1 column of 12 blocks each containing 32x12 unique spots.
The duplicates spots are printed in an identical column next to the first one. Spotting time to print 36 slides takes approximately 4 hours. In each of the 12 blocks, 32 Universal ScoreCard controls (Amersham Biosciences Buckinghamshire, UK) are printed making a total of 384 controls spotted in duplicate.

[2]. Array protocol description:
Equipment: 1. Generation III microarray spotter (Amersham Biosciences, Buckinghamshire, UK). 2. 384 microtiter plates (Amersham Biosciences, Buckinghamshire, UK). 3. UV-crosslinker (Biolink, BLX-254, Vilber Lourmat, France). 4. Type VII silane coated slides (cat#: RPK0174, Amersham BioSciences, Buckinghamshire, UK). Spotting: 1. Dissolve cDNA array elements in 50% DMSO at concentrations ranging between 100-200ng/µl and array in 384 microtiter plates. 2. Set spotter settings for 4608 spots in duplicate: normal mode, 55% humidity, spot diameter: 250, spot buffer: 30, and pen deflection:0.508mm. 3. Make sure room temperature is between 20-24 degrees Celsius. 4. Place microtiter plates in the plate hotel with A1 in upper right corner. 5. Place 36 slides on the slide-deck and perform 2 manual pin-washes. 6. After spotting, let slides dry at 55% humidity for 3 hours and cross-link at 50 mjoules. 7. Block slides in %SSPE for 30 minutes at room temperature rinse in water and dry in centrifuge. 8. Slides can be stored for at least 6 months in a dry-cabinet.

first: Paul

last: Van Hummelen


title: Synechocysis sp. PCC6803 full genome array version 1

status: Public on Apr 28 2004

city: Stillwater

first: Robert

last: Burnap

state: OK

institute: Oklahoma State University

organism: Synechocystis sp. PCC 6803

description: Construction and Preparation of Synechocystis Spotted Microarrays:  3168 genes or putative ORFs of Synechocystis sp. PCC6803 were PCR amplified in two stages.  Primary amplification was achieved using gene-specific up-stream and down-stream primers modified with adaptamer sequences and genomic DNA template.  Secondary amplification was achieved using adaptamer primers and individual primary PCR product templates.  The quality of the PCR products was determined by agarose gel electrophoresis.  The PCR products were purified using Millipore Multi-screen plates and resuspended in Micro Spotting buffer (TeleChem; Sunnyvale, CA) to a concentration of 500ng/ul.  Each probe was then printed (spotted) in triplicate onto TeleChem SuperAmine substrates at room temperature and 50% relative humidity using a GeneMachines Omnigrid 100 (Genomic Solutions; Ann Arbor, MI) robot fitted with 4x4 TeleChem SMP4 pins.  After printing, each slide was rehydrated by holding the slide with the array side down over a container of steaming water for 1 s and snap-dried on a warm hot plate.  Printed slides were baked at 80°C for 1 h and then subjected to a UV-cross-linking at a dose of 1500 mJ cm2.  Slides with immobilized probes were then stored in slide holders and placed in a desiccator at room temperature.

first: Bradley

last: Postier

first: Patricia

last: Ayoubi


title: NHGRI Custom cDNA Microarray

status: Public on Jun 24 2004

city: Gaithersburg

first: Jeff

last: Kiefer

state: MD

address: 20 Firstfield Rd,  Suite 110

department: CDDL

institute: Translational Genomics Research Institute (TGen)

description:
This cDNA microarray encompasses ~16,000 genes printed in the Microarray Core of the the National Human Genome Research Institute (NHGRI)/NIH. These cDNA microarrays were generated from cDNA clones obtained from the sequence verified IMAGE clone selection from Research Genetics (Invitrogen corporation, Inc). Of these clones, 75% represent genes with functional annotation and the remaining represent ESTs and hypothetical proteins. The gene description of these clones was based on the Unigene database, build #148 and the chromosomal base location of these genes was updated from University of California Santa Cruz’s (UCSC) Genome Browser database. This update was performed through the GenBank accession ID of the Image clone ID. For quality control, 2 slides per lot were hybridized for spot consistency, homogeneity and signal to noise ratios.
Keywords = cDNA Microarray


title: VIB Mouse 5K 2

description:
[1]. Array design:
This microarray contains 4608 array elements spotted in duplicate.
Since the microarray spotter is equipped with 12 capillary pins, the spots are arrayed over the entire length of the slide in 1 column of 12 blocks each containing 32x12 unique spots. The duplicates spots are printed in an identical column next to the first one. Spotting time to print 36 slides takes approximately 4 hours.
In each of the 12 blocks, 32 Universal ScoreCard controls
(Amersham Biosciences Buckinghamshire, UK) are printed
making a total of 384 controls spotted in duplicate.

[2]. Array protocol description:
Equipment: 1. Generation III microarray spotter (Amersham Biosciences, Buckinghamshire, UK). 2. 384 microtiter plates (Amersham Biosciences, Buckinghamshire, UK). 3. UV-crosslinker (Biolink, BLX-254, Vilber Lourmat, France). 4. Type VII silane coated slides (cat#: RPK0174, Amersham BioSciences, Buckinghamshire, UK). Spotting: 1. Dissolve cDNA array elements in 50% DMSO at concentrations ranging between 100-200ng/µl and array in 384 microtiter plates. 2. Set spotter settings for 4608 spots in duplicate: normal mode, 55% humidity, spot diameter: 250, spot buffer: 30, and pen deflection:0.508mm. 3. Make sure room temperature is between 20-24 degrees Celsius. 4. Place microtiter plates in the plate hotel with A1 in upper right corner. 5. Place 36 slides on the slide-deck and perform 2 manual pin-washes. 6. After spotting, let slides dry at 55% humidity for 3 hours and cross-link at 50 mjoules. 7. Block slides in %SSPE for 30 minutes at room temperature rinse in water and dry in centrifuge. 8. Slides can be stored for at least 6 months in a dry-cabinet.


title: NMC 7.7k duplicate human cDNA arrays, 020617

status: Public on Dec 06 2003

city: Trondheim

first: Kristin

last: Nørsett

department: Dept of Cancer Research and Molecular Medicine

country: Norway

institute: Norwegian University of Science and Technology

description: The 7.7k human cDNA arrays used here are part of a larger collection of 30k cDNAs purchased from Research Genetics (Huntsville, AL).  Plasmids were isolated from overnight bacterial cultures in 96-well plates and cDNA inserts amplified by PCR using M13 primers 5'-gtt gta aaa cga cgg cca gtg and 5'-cac aca gga aac agc tat g. The PCR products were precipitated with ethanol, washed and redissolved in 3x SSC (sodium dodecyl sulphate). Quality and concentration was assessed by agarose gel electrophoresis. The purified PCR products were prepared in 50% DMSO (about 50-200 ng/ l) and spotted in duplicate onto 25 x 75mm amino-silane coated CMT-GAPS glass slides (Corning, NY) using a MicroGrid II printing robot (BioRobotics; Cambridge, UK). The arrays were spotted with 8 pins and contained 4 x 8 blocks of 22 x 22 spots. The probes were fixed to the slide surface by UV irradiation. The 7.7k cDNA probes represent 7561 unique UniGene clusters, all printed in duplicates. In addition, a few in-house cDNA probes and a set of positive and negative control elements including 10 different PCR products of exogenous DNA from SpotReport™ cDNA Array Validation System (Stratagene, La Jolla, CA) were printed on the arrays. Totally, each array contained 15488 elements including duplicates and controls.

middle: G

first: Astrid

last: Lægreid

first: Wacek

last: Kusnierczyk

first: Mette

last: Langaas

first: Sonja

last: Ylving

first: Simen

last: Myhre

first: Helge

last: Waldum

first: Arne

last: Sandvik


title: AR9.2K

status: Public on Feb 25 2004

city: New Haven

first: E. Janet

last: Hager

state: CT

address: 300 George Street, Suite 2110

department: W.M. Keck Foundation Biotechnology Resource Laboratory

institute: Yale University School of Medicine

organism: Arabidopsis thaliana

description: Arabidopsis AR9.2K is a cDNA array generated from MSU clone set. The array contains 9,216 clones, single spot, 175 µm center-to-center distances, 2 arrays per slide.

first: Janet


title: Incyte Human UniGEM V 2.0 (GEM-5112)

status: Public on Sep 20 2003

city: Syracuse, New York

first: Lilia

last: Maglova-Crowe

state: NY

address: 130 College Place, BRL

institute: Syracuse University

description:
Genome Systems Inc. Catalog # GEM-5112 - Human UniGEM V 2.0 Custom Screen.

The Incyte Human UniGEM V 2.0 (GEM-5112) gene expression microarray contained 9,182 elements/8,556 unique human cDNA genes and ESTs. The clones are derived from Incyte’s set of sequence-verified cDNA clones and represent unique human genes identified in NCBI’s UniGene database. For more information see: Agilent Technologies Human 1 cDNA Array Gene List http://www.chem.agilent.com/cag/bsp/cDNAGL/human1_clonesetB2.htm
Keywords = high density cDNA microarray

last: Maglova

first: William

middle: E

last: Crowe

first: John

last: Russell


title: GeneTrack® Human cDNA chip HSVC303

status: Public on Aug 05 2003

city: Daejon

first: Park

last: Jinwoo

country: South Korea

institute: TOOLGEN

description:
GeneTrack® Human cDNA chip
7.5K : Cat No HSVC 303


title: Incyte Human UniGEM V .14

status: Public on Jun 28 2003

city: Dallas

first: Khurram

middle: S

last: Rehman

address: 5323 Harry Hines Blvd

department: Obstetrics and Gynecology

institute: UT Southwestern Medical Center

description:
The UniGEM V gene expression microarray contains 7,075 sequence-verified human cDNA clones for both known genes and ESTs. UniGEM V clones include a broad spectrum of human genes whose responses can be measured for all types of experimental systems. The clones are derived from Incyte’s set of sequence-verified cDNA clones and represent unique human genes identified in NCBI’s UniGene database, build 46. For more information about UniGene, visit http://www.ncbi.nlm.nih.gov/UniGene/
Keywords = high density cDNA microarray

first: Su

last: Yin

first: Bobbie

middle: A

last: Mayhew

first: R. Ann

last: Word

last: Rainey


title: Incyte Human UniGEM V 1.0 (GEM-5100, UniGene build 75)

description:
Genome Systems Inc. Catalog # GEM-5100 - Human UniGEM V 1.0 Custom Screen.

The Incyte Human UniGEM V 1.0  gene expression microarray contained 7,075 elements/6,794 unique human cDNA genes and ESTs. The clones are derived from Incyte’s set of sequence-verified cDNA clones and represent unique human genes identified in NCBI’s UniGene database, build 75. For more information see: Agilent Technologies Human 1 cDNA Array Gene List http://www.chem.agilent.com/cag/bsp/cDNAGL/human1_clonesetB2.htm
Keywords = high density cDNA microarray


title: MWG Human Cancer Array

status: Public on Oct 07 2003

city: High Point

last: MWG Biotech

state: NC

address: 4170 Mendenhall Oaks Parkway, Suite 160

institute: MWG Biotech

description: The MWG Human Cancer Array represents 1.900 genes associated with cancer development and progression and is the most complete microarray solution for cancer research available on the market so far. Sophisticated bioinformatics not only fulfill the criteria of chip design specificity and sensitivity, but also reduce an unnecessary flood of data to the essential - an advantage customers of other chip manufacturers do not have. MWG's patented HPSF® synthesis technology and strict quality control deliver the highly purified and errorless oligonucleotides, which have been developed by the department of bioinformatics before. Information Sources: 1,660 genes were referenced by NCBI's Online Inheritance in Man (OMIN) as cancer related (147 were also identified as differentially expressed in cancer from Serial Analysis of Gene Expression (SAGE)). A wide range of cancer specific genes are represented on the chip. Key words were searched in the literature to identify important genes in common cancer types including: Carcinoma specific genes: colon, breast, prostate, lung, bladder, kidney, liver, pancreas, thyroid, nasopharyngeal, esophagus, ovary; Melanoma specific genes including MAGE; Neuroblastomas; Leukemia, lymphoma and sarcoma specific genes including cancer specific translocations; Genes involved in cell cycle control, cell adhesion, angiogenesis, signaling and immunity.

distribution: commercial

manufacture_protocol: see manufacturer's web site


title: Agilent Rat cDNA Microarray (G4105A) [layout A]

status: Public on Jan 02 2004

city: Palo Alto

last: Agilent Technologies

state: CA

institute: Agilent Technologies

organism: Rattus norvegicus

description:
14,355 unique clones
Sourced from Incyte's LifeSeq® Foundation 1 & 2 clone sets
Barcode location:Bottom
Scan Side:Front

This platform should be used for the following prints:

000472_D_20020516_FSPortraitBcodeDown
000472_D_20030121_FSPortraitBcodeDown

Please reference this platform if you used an array with the same layout, even if the print lot is different than specified above. The GEO staff will update the description of this platform to include additional print lots as encountered.

Print-specific information should be accounted for in the sample hybridization tables (eg. NULL value insertions for spots to be ignored).


title: Array of ESTs from F. verticillioides subtraction libraries

status: Public on Dec 20 2003

city: West Lafayette

first: Charles

middle: P.

last: Woloshuk

state: IN

institute: Purdue University

organism: Fusarium verticillioides

description: In the study, expression profiles in wild type and a fcc1 mutant were compared to identify genes with patterns of expression similar to the FUM genes.  DNA microarrays were constructed with ESTs from cDNA libraries obtained from the fcc1 mutant and wild-type strain of F. verticillioides grown on maize kernels . By microarray methodology, gene expression was measured in the fcc1 mutant and the wild type during growth on maize and in a synthetic medium at pH 3 and 8. The results of the analysis provide potential candidate genes for future studies to determine their functional roles in fumonisin biosynthesis.

first: Anna Maria

last: Pirttilä

first: Lauren

last: McIntyre

first: Gary

last: Payne

middle: P


title: Heidelberg FlyArray

status: Public on Dec 31 2003

city: Heidelberg

first: Boris

last: Beckmann

address: Im Neuenheimer Feld 580

department: Funktionelle Genomanalyse

country: Germany

institute: Deutsches Krebsforschungszentrum

organism: Drosophila melanogaster

description:
cDNA Microarray from spotted PCR products containing 47,616 features from Drosophila melanogaster and various controls. Primer design was made with GenomePride software. Average length is 500 bp within exonic sequence.
Microarray construction
The 2nd round PCR-product was spotted in 3x SSC, 150mM NaPO4, 1.5M betaine onto QMT Amino slides (Quantifoil, Jena, Germany)
using a MicroGrid II arrayer (BioRobotics, Cambridge, UK) and SMP3 pins (TeleChem International Inc., Sunnyvale, USA).
Each PCR-product was spotted twice at different positions on the microarray. As controls, PCR-products of Arabidopsis cDNAs,
genomic Drosophila DNA and C. elegans cDNAs were spotted. The DNA was UV-crosslinked (250mJ/cm2) and baked for 4h at 80°C.
In total, the Heidelberg FlyArray contains 47,616 features, representing the 21,306 ORF-amplicons and 2,502 controls.

first: Marc

last: Hild

first: Stefan

last: Haas

first: Britta

last: Koch

first: Victor

last: Solovyev

first: Christian

last: Busold

first: Kurt

last: Fellenberg

first: Michael

last: Boutros

first: Martin

last: Vingron

first: Frank

last: Sauer

first: Joerg

last: Hoheisel

first: Renato

last: Paro


title: GeneTrack® Human cDNA chip 7.5k

status: Public on Dec 12 2003

first: Jin-Soo

last: Kim

institute: ToolGen, Inc.

description:
7.5k Human EST clones spotted
(www.genomictree.com)


title: UHN Microarray Centre – Human 19K 2ii (Part B)

status: Public on Dec 07 2003

city: Ottawa

first: Felix

middle: M.

last: Mesak

state: ON

address: 503 Smyth Rd. 3rd Floor

department: Centre for Cancer Therapeutics

institute: Ottawa Regional Cancer Centre

description:
cDNA microarrays are printed on CMT-GAPS slides (Corning Inc.) using high precision robotics. Contains 19008 characterized and unknown human ESTs. The 19k array is split over a pair of slides (Part A and Part B). Each EST was spotted in duplicate. This platform is the 19k array version 2ii Part B. The cDNA microarray can be purchased at University Health Network (UHN) Microarray Centre, Ontario Cancer Institute (OCI), Toronto, Canada [ http://www.microarrays.ca ].
Keywords = cDNA microarray, ESTs

first: Cheng

last: Ng


title: UHN Microarray Centre – Human 19K 2ii (Part A)

description:
cDNA microarrays are printed on CMT-GAPS slides (Corning Inc.) using high precision robotics. Contains 19008 characterized and unknown human ESTs. The 19k array is split over a pair of slides (Part A and Part B). Each EST was spotted in duplicate. This platform is the 19k array version 2ii Part A. The cDNA microarray can be purchased at University Health Network (UHN) Microarray Centre, Ontario Cancer Institute (OCI), Toronto, Canada [ http://www.microarrays.ca ].
Keywords = cDNA microarray, ESTs


title: VIB Mouse 5K 4

first: Rekin's

last: Janky

description:
[1]. Array design:
This microarray contains 4608 array elements spotted in duplicate. Since the microarray spotter is equipped with 12 capillary pins, the spots are arrayed over the entire length of the slide in 1 column of 12 blocks each containing 32x12 unique spots. The duplicates spots are printed in an identical column next to the first one. Spotting time to print 36 slides takes approximately 4 hours.  In each of the 12 blocks, 32 Universal ScoreCard controls  (Amersham Biosciences Buckinghamshire, UK) are printed making a total of 384 controls spotted in duplicate.

[2]. Array protocol description:
Equipment: 1. Generation III microarray spotter (Amersham Biosciences, Buckinghamshire, UK). 2. 384 microtiter plates (Amersham Biosciences, Buckinghamshire, UK). 3. UV-crosslinker (Biolink, BLX-254, Vilber Lourmat, France). 4. Type VII silane coated slides (cat#: RPK0174, Amersham BioSciences, Buckinghamshire, UK). Spotting: 1. Dissolve cDNA array elements in 50% DMSO at concentrations ranging between 100-200ng/µl and array in 384 microtiter plates. 2. Set spotter settings for 4608 spots in duplicate: normal mode, 55% humidity, spot diameter: 250, spot buffer: 30, and pen deflection:0.508mm. 3. Make sure room temperature is between 20-24 degrees Celsius. 4. Place microtiter plates in the plate hotel with A1 in upper right corner. 5. Place 36 slides on the slide-deck and perform 2 manual pin-washes. 6. After spotting, let slides dry at 55% humidity for 3 hours and cross-link at 50 mjoules. 7. Block slides in %SSPE for 30 minutes at room temperature rinse in water and dry in centrifuge. 8. Slides can be stored for at least 6 months in a dry-cabinet.

first: Paul

last: Van Hummelen


title: VIB Mouse 5K 3

description:
[1]. Array design:
This microarray contains 4608 array elements spotted in duplicate. Since the microarray spotter is equipped with 12 capillary pins, the spots are arrayed over the entire length of the slide in 1 column of 12 blocks each containing 32x12 unique spots. The duplicates spots are printed in an identical column next to the first one. Spotting time to print 36 slides takes approximately 4 hours.
In each of the 12 blocks, 32 Universal ScoreCard controls
(Amersham Biosciences Buckinghamshire, UK) are printed
making a total of 384 controls spotted in duplicate.

[2]. Array protocol description:
Equipment: 1. Generation III microarray spotter (Amersham Biosciences, Buckinghamshire, UK). 2. 384 microtiter plates (Amersham Biosciences, Buckinghamshire, UK). 3. UV-crosslinker (Biolink, BLX-254, Vilber Lourmat, France). 4. Type VII silane coated slides (cat#: RPK0174, Amersham BioSciences, Buckinghamshire, UK). Spotting: 1. Dissolve cDNA array elements in 50% DMSO at concentrations ranging between 100-200ng/µl and array in 384 microtiter plates. 2. Set spotter settings for 4608 spots in duplicate: normal mode, 55% humidity, spot diameter: 250, spot buffer: 30, and pen deflection:0.508mm. 3. Make sure room temperature is between 20-24 degrees Celsius. 4. Place microtiter plates in the plate hotel with A1 in upper right corner. 5. Place 36 slides on the slide-deck and perform 2 manual pin-washes. 6. After spotting, let slides dry at 55% humidity for 3 hours and cross-link at 50 mjoules. 7. Block slides in %SSPE for 30 minutes at room temperature rinse in water and dry in centrifuge. 8. Slides can be stored for at least 6 months in a dry-cabinet.


title: Gm-r1070

status: Public on Mar 31 2003

city: Urbana

first: Lila

middle: O.

last: Vodkin

address: 1201 W. Gregory Dr.

department: Crop Sciences

institute: University of Illinois

organism: Glycine max

description:
9,216 single-spotted cDNA clones from embryo,
seed coat, flower and whole pod libraries
Keywords = soybean

middle: O

last: Keim

first: Randy

last: Shoemaker

first: Ernest

last: Retzel

first: Anupama

last: Khanna

first: Virginia

last: Coryell

first: John

last: Erpelding

first: Steven

last: Clough

first: Francoise

last: Thibaud-Nissen

first: Robin

middle: T

last: Shealy

first: Elizabeth

last: Shoop

first: Martina

last: Stromvik