title: Nimblegen Haemophilus influenzae Rd KW20 array 2236

status: Public on Jul 28 2006

city: Oklahoma City

first: Timothy

middle: M

last: VanWagoner

state: OK

address: 940 SL Young Blvd, BMS340

department: Pediatrics

country: USA

institute: University of Oklahoma Health Sciences Center

organism: Haemophilus influenzae Rd KW20

technology: in situ oligonucleotide

distribution: custom-commercial

first: Paul

middle: W

last: Whitby

first: Thomas

last: Seale

first: Daniel

middle: J

last: Morton

first: Terrence

middle: L

last: Stull

manufacture_protocol:
Oligonucleotide arrays were synthesized by NimbleGen Systems, Inc by maskless array synthesis using a digital micromirror array-mediated, parallel synthesis process incorporating 5’-photoprotected phosphoramidites as previously described. (1)  Synthesized arrays were deprotected by incubation in ethanolamine:ethanol (1:1) at room temperature for two hours, washed extensively in distilled water, and stored desiccated until used.

1:4 catalog design of 1657 genes from H. influenza Rd KW20 with 19 pp/gene and 3x replication.  Design includes ~5400 random GC probes.


title: Sigma Genosys Panorama Escherichia coli Gene Array

status: Public on Dec 20 2002

city: The Woodlands

last: Sigma-Genosys

state: TX

address: 1442 Lake Front Circle

institute: Sigma-Genosys

organism: Escherichia coli

technology: spotted DNA/cDNA

description: The Panorama Escherichia coli Gene Array was developed using E. coli ORFmers (PCR primers). The PCR primers were developed as part of a collaboration with the E. coli Genome Group, headed by Dr. Fred Blattner at the Department of Genetics, University of Wisconsin-Madison (http://www.genome.wisc.edu).  The array contains 4,290 PCR-amplified ORFs, representing all of the protein-encoding genes.

distribution: commercial

manufacture_protocol: see manufacturer's web site


title: GLYCOv2 glycogene array

status: Public on Nov 15 2004

city: La Jolla

first: Steven

last: Head

state: CA

address: 10550 N Torrey Pines Rd

institute: The Scripps Research Institute

organism:
Homo sapiens
Mus musculus

technology: spotted oligonucleotide

description:
For analysis of data from human samples, ignore all mouse probe set data. For analysis of mouse data, ignore all human probe set data.
A custom-designed Glyco-gene Chip array that contains probe sets to monitor the expression of human and mouse transcripts relevant to the Consortium for Functional Glycomics.The following classes of genes are represented on the GLYCOv2 Gene Chip: Glycosyltransferases Glycan-binding proteins (GBP) Sulfotransferases C-type lectins Neuraminidases Nucleotide sugar synthesis and transporter proteins N-glycan biosynthesis related genes Mucins Proteoglycans Growth factors Cytokines Chemokines and Conserved oligomeric Golgi (COG) complex genes. Many of the probe sets are represented up to three times on the array. Replicate probe sets are all indicated by a notation of the copy in the probe set ID.
Available as a resource throught the Consortium of Functional Glycomics. See this URL for complete information http://web.mit.edu/glycomics/consortium/
Many of the the probe sets on this array are represented in either two or three identical copies. Probe sets with more than one copy on this array contain the text: "Copy1", "Copy2" or "Copy3" within the probe set name.
The .CDF file for this array may be obtained by contacting Steven Head (shead@scripps.edu) or Timothy Gilamrtin (timgil@scripps.edu) or through the TSRI DNA Array Core Facility (http://www.scripps.edu/services/dna_array/).

last: Gilmartin

middle: R

first: James

middle: C

last: Paulson

first: Elena

last: Comelli


title: Bacillus cereus ATCC14579 design1

status: Public on Apr 10 2007

city: Wageningen

first: Menno

middle: van der

last: Voort

address: Bomenweg 2

department: Agrotechnology and Food Sciences Group

country: Netherlands

institute: Wageningen UR

organism: Bacillus cereus ATCC 14579

manufacture_protocol:
Agilent manufacture protocol.
Agilent uses its inkjet technology to print oligos onto glass slides.


title: NMC Human 35k oligo array v2.1

status: Public on Apr 13 2007

city: Tromsø

first: Ruth

middle: Hracky

last: Paulssen

address: MH-Bygget Breivika

department: Institute of Clinical Medicine

country: Norway

institute: Universitetet i Tromsø

organism: Homo sapiens

description:
Consists of 36,288 spots printed with 48 pins (6x2 cm) The Human Genome Oligo Set Version 3.0 (OPERON) contains 34,580 70mer probes representing 24,650 genes and 37,123 gene transcripts.
mirVana miRNA array 1.3

distribution: non-commercial

manufacturer: Norwegian Microarray Consortium, Radium-Rikshospitalet/ UiO Node

middle: H

manufacture_protocol:
Spotting buffer: 50% DMSO Slide surface: CMT UltraGAPS Spot size: 100 µm Spot distance: 155 µm Internal controls:Hum oligo Control (OPERON),Lucidea Microarray ScoreCard v1.1(Amersham Biosciences),SpotReport oligo validation system (Stratagene),Stringency controls (home made).
Attachment type: covalent
Strandness type: single


title: Agilent 22K custom mouse gene expression array 2 of 2

status: Public on Apr 18 2007

city: Toronto

first: Emily

last: Walker

state: ON

address: 164 College Street Room 407

department: Institute for Biomaterials and Biomedical Engineering

country: Canada

institute: University of Toronto

organism: Mus musculus

first: Wen

last: Zhang

last: Hughes

first: William

last: Stanford

manufacture_protocol: Hydrophobic glass wafers (75 × 75 mm) containing exposed hydroxyl groups for nucleotide coupling were prepared using a selfassembling silane mixture to form a uniform, hydrophobic surface with a controlled density of hydroxyl groups extending by a single methylene unit above unfunctionalized silane components. Phosphoramidite monomers in propylene carbonate were delivered to defined positions on the glass surfaces using commercial ink-jet printer heads. Synthesis cycles were otherwise similar to traditional oligonucleotide synthesis, with the exception that capping was omitted. After manufacture, arrays were diced to 25 × 75 mm to accommodate commercial scanners.


title: HMC Human 29k Array

status: Public on Apr 30 2007

city: Odense

first: Mads

last: Thomassen

address: Soender Boulevard 9

department: Clinical Genetics (KKA)

country: Denmark

institute: Odense University Hospital

first: Qihua

last: Tan

first: Freyja

last: Eiriksdottir

first: Martin

last: Bak

first: S

last: Cold

first: Torben

middle: A

last: Kruse

manufacture_protocol: Biochem Biophys Res Comm 2006; 344: 1111-1120


title: University of Groningen Lactococcus lactis MG1363 amplicon 5K

status: Public on Apr 17 2007

city: Groningen

first: Anne

middle: de

last: Jong

address: Nijenborgh 7

department: Molecular Genetics

institute: university of Groningen

organism: Lactococcus cremoris subsp. cremoris MG1363

description:
LOCUS       AM406671             2529478 bp    DNA     circular BCT 31-JAN-2007
DEFINITION  Lactococcus lactis subsp. cremoris MG1363, complete genome.
ACCESSION   AM406671
VERSION     AM406671.1  GI:124491690
KEYWORDS    complete genome.
SOURCE      Lactococcus lactis subsp. cremoris MG1363
The complete genome sequence of the lactic acid bacterial paradigm Lactococcus lactis subsp. cremoris MG1363.
J Bacteriol. 2007 Feb 16

last: de Jong

first: Oscar

last: Kuipers

first: Jan

last: Kok

manufacture_protocol: Amplicon spotted on ArrayIt Superamine slides in duplicates


title: M1 and M18 array

status: Public on Apr 27 2007

city: New York

first: Patricia

middle: A.

last: Ryan

state: NY

address: 1230 York Ave

department: Bacterial Pathogenesis and Immunology

institute: Rockefeller University

organism: Streptococcus pyogenes

manufacturer: Fischetti Laboratory, Rockefeller University


title: Eurogentec S. pombe IGR+ORF array (J120E) for Lowess norm

status: Public on Apr 12 2007

city: Stockholm

first: Karl

last: Ekwall

address: Alfred Nobels Alle 7

country: Sweden

institute: Karolinska Inst

organism: Schizosaccharomyces pombe

manufacture_protocol:
Primary PCR: The PCR reactions are performed using tagged genome specific primers in the presence of genomic DNA (provided by your laboratory). We normally achieve a success rate of 93 % of PCR reactions. For the failed PCR reactions we perform three different protocols of PCR conditions, which include different MgCl2 concentrations and different Taq polymerase. We should normally get an additional 2 % working and reach a success rate of 95 % in total. We check 5 % of the total oligos by Mass Spectrometry and will re-synthesize maximum 2 % of the oligos based on a newly designed primer. We will perform the PCR reactions with the new designed oligonucleotides with the standard PCR protocol. Following this process we reach usually 97 % success rate.
Secondary PCR: amplifications will be performed using a single pair of primers annealing to tag sequences introduced on primary PCR primers. These PCR amplifications will also be controlled by agarose gel electrophoresis and repeated if required reaching a minimum of 95 % success rate. The final PCR products will contain an amino group at their 5' end to promote the attachment of the predicted gene coding DNA strand to the treated glass slides.


title: McMahon_Mouse_44K_Neural

status: Public on Apr 20 2007

city: Austin

first: Steve

last: Vokes

state: Texas

address: 2500 Speedway

department: Molecular Biosciences

institute: University of Texas at Austin

first: Steven

first: Hongkai

last: Ji

first: Wing

last: Wong

first: Andrew

middle: P

last: McMahon

first: Scott

last: McCuine

first: Shane

last: Giles

manufacture_protocol: Spotted 60-mers printed with Ink-jet printers


title: ISB yeast ORF 2642-2665

city: Seattle

first: Jennifer

middle: Joy

last: Smith

state: WA

address: 1441 North 34th Street

institute: Institute for Systems Biology

organism: Saccharomyces cerevisiae

description: Spotted oligo array of the 6308 70-mer probes purchased from QIAGEN/Operon.

first: John

middle: D

last: Aitchison

first: Bruz

last: Marzolf

manufacture_protocol:
MIAME platform type: spotted
MIAME availability: Arrays printed at ISB can be purchased, contact bmarzolf@systemsbiology.org for information.
Standard Operating Procedures for the Virtek(ESI) Spotter
*** Always remember that spotter pins are extremely fragile at the tips ***
To make spotter source plates:
1. Total volume should be 4-8 ul
2. Usually, DNA should be in 3X SSC and 1.5M betaine. This can be done most accurately be putting an appropriate amount of source material in spotting plates, then drying down with the speedvac and resuspending in the final volume of 3X SSC, 1.5M betaine.
Things not to do:
1. Move slides after printing starts ? just touching a slide can shift it and other slides enough to ruin the grid geometry.
2. Use air to blow dust off of slides - you could move the slides
3. Press the RED EMERGENCY button if it's not a emergency- this will turn off the spotter controller and abort the entire run
4. Use PCR products that have been purified, then dried down and resuspended in a very small volume, this is known to cause erratic surface tension and cause spots to run together.
When starting a new print run:
1. Allow blank slides to sit at 65% humidity for 24 hours
2. Renew sticky mat if it's dirty
3. Fill humidifier and turn on
4. Pull water out of water bath, wipe out bath and fill reservoir to get bath back to correct level- IT IS EXTREMELY IMPORTANT NOT TO OVERFILL THE WATERBATH OR SONICATOR BATH, WATER SHOULD BE JUST ABOVE THE POINT WHERE CUT RUNNING UP THE PIN ENDS
5. Place slides on the deck with label on the right-hand side. Secure slides by using spacer glass slides held in place with the magnets at the end of the platform.
6. Measure total X and Y distances spanned by slides, enter sizes under Calibration for Arraying->Slides
7. Print one "dip" with no source plate to ensure that pins are hitting all slides in the same plate (slight errors in Y direction especially can really throw off grid locations.)
8. Use the metal brace and spacer slides held by magnets to stabilize and line up the slides
9. Stabilize humidity at approximately 65%
10. Put blotting slide in place
To print slides:
1. Spin down 4 source plates at 2000 rpm for one minute
2. Put three remaining source plates (in order) at 4 C
3. Check blotting slide for blocked pins, change slide. Remove clogged/sticking pins and try to remedy by cleaning pin and/or printhead hole. If a pin isn't spotting on the blotter, it may just not be contacting the blotter.see if it's been spotting on actual slides.
4. Change water bath water at beginning of run.
5. Check level of water reservoir and sonicator bath- add water if necessary
6. Check humidity
7. Double-check orientation and number of source plate
8. Press operator button
9. Re-seal old plate and put in cold room
10. Set timer
11. Check old plate out, new plate in on printing checklist
When finished printing for the day:
1. Seal all plates and place in cold room
2. Turn off Spotter light
3. Turn monitor off
4. Leave computer ON
When finished with entire printing:
1. Allow spotter humidity to slowly drop and slides dry out.
2. Remove slides and place into plastic Telechem slide boxes.
3. Turn off Spotter light
4. Turn off fan/humidifier
5. Place boxes in desiccator at least 12 hours.
6. Blow off slides in boxes, then bake at 85 C for 2 hours.
7. Etch numbers and corners on slides (corners on back side), blow dry and place in desiccator ready for prehyb.
Things to do periodically:
1. Just keep an eye on the slides themselves to see if the grids are being formed as expected
To clean a clogged pin:
1. Remove pin by pulling out from top with a small forceps.
2. Dip in water and blow dry with air tip, repeatedly if necessary.
3. Make sure water reservoir is sufficiently full - low water often causes clogging.
Random experiments that we have tried and their results:
1. Effect of Temperature and Humidity on Spot Size
Printed H-70 in 3X SSC onto Telechem Amine slides. Temperatures chosen were 18 C, 20 C, 22 C, and humidities of 40%, 50%, 60% resulting in 9 different conditions. Hybridization was with labeled PCR primer for 3 hours.
Results: Differences were seen in specific grids, but grid-grid variability was too high to establish a conclusive pattern. Spots generally were most intense in a 90-100 micron diameter, then had a lighter "halo" that could extend to 130-150 microns. It appeared that "Halo" size was often smallest at 18 C, 130-140 microns. We decided to print at ambient temperature, generally 18-20 C.
2. Effect of Volume and Humidity on Maximum Spots per Dip
Pooled 96 wells of purified PCR product and added SSC for a final concentration of 3X. Then set up a plate so that 8 pins would print 1ul, another 8 would print 2ul, 4ul, 6ul, 8ul and 10ul. This 48-pin dip was then printed 25 spots in the Y direction, across 10 plain glass slides for a total of 250 spots. This print was done with increasing indent at 75%, 70%, 65%, 60% and 55% humidity.
Results: At 1-2ul, pins ran out of juice at 25-50 spots at all humidities. At 4ul, 200-225 spots could be printed at 65% or higher humidity. With 6ul or more, 250 spots could be printed at 65% or higher humidity. Based upon this, we chose 65% humidity for all printing.
Halobacterium Flip Chips: This print is outdated
Use 24 pins (4Y by 6X), 16Y by 16X grids at 200 micron spot-spot spacing.
Plate order: 1-7, Control, Control(rotated), 7(rot), 6(rot), 5(rot), 4(rot), 3(rot), 2(rot), 1(rot)
Typical number of slides printed: 36 (strange layout used to get 2 identical "supergrids" limited to 2 columns of 18)
This layout uses the same "2 supergrid" layout as Yeast, except that there are not duplicates located directly next to each other. Instead, after the plates have been printed normally, they are printed in reverse order and rotated, placing duplicates in a supergrid in exact opposite corners.


title: JCL_Ecoli_1

status: Public on Apr 26 2007

city: Logan

first: Daniel

last: Hyduke

state: UT

address: 4105 Old Main Hill ENGR 402S

department: Biological Engineering

institute: Utah State University

manufacturer: James C. Liao's Metabolic Engineering Laboratory, UCLA

first: Laura

last: Jarboe

first: James

last: Liao

manufacture_protocol: Oligonucleotides from Operon AROS E. coli set, were printed on Corning UltraGAPS slides.


title: TJU-DBI Rat array v3.2

status: Public on Apr 06 2007

city: Philadelphia

first: Egle

last: Juskeviciute

state: PA

address: 1020 Locust st. JAH 527

department: Pathology

institute: Thomas Jefferson University

organism: Rattus norvegicus

manufacture_protocol:
ResGen clones for Rat
Tip Configuration: Standard 48 tip
Columns per Subarray: 20
Rows per Subarray: 19
Column Spacing: 225
Row Spacing: 225


title: Custom Nitrosomonas europaea GeneChip Array (Affymetrix)

status: Public on Apr 02 2007

city: Corvallis

first: barbara

last: gvakharia

state: OR

address: Cordley Hall 4097

department: Botany and Plant Pathology

institute: OSU

organism: Nitrosomonas europaea

description: The array contains 192, 000 features. Probe length-24nt. Probes/gene-13.

first: Barbara

middle: O

last: Gvakharia

last: Arp

first: Luis

last: Sayavedra-Soto

first: Peter

last: Bottomley


title: China CDC Shigella flexneri cDNA array

status: Public on Apr 07 2007

city: Beijing

first: Hua

last: Fu

address: 6#, Rongjing East Street

country: China

institute: Institute of pathogen biology

organism: Shigella flexneri

manufacturer: State Key Laboratory for Molecular Virology and Genetic Engineering, Chinese Center for Disease Control and Prevention

manufacture_protocol: The cDNA microarrays have 3943 PCR-amplified sf301 (S. flexneri 301 strain) open reading frames (ORFs) and other 409 spots represented as controls. Excluding replicated spots for 57 ORF, the microarrays allowed us to analyze the expression of 3771（93.3%） chromosome coded ORFs and 113 (72.9 %) plasmid-borne ORFs. Sf301 ORFs were amplified with ORF-specific primer pairs (Invitrogen). Amplified products which mostly ranged between 300 and 700 bases in length were purified by using MultiScreen-PCR plates (Millipore). Concentrations of the PCR products were measured by Spectra MAX 190 (Molecular Devices).Then the PCR products were readjusted to 100 ng/ul of concentration with deionized water and double diluted into spotting solution containing 60% DMSO. The PCR products were spotted onto gamma amino propylsilan coated GAPII slides (Corning) by using OmniGrid ™ microarrayer (GeneMachines).


title: Agilent 22K custom mouse gene expression array 1 of 2

first: Emily

last: Walker

first: Wen

last: Zhang

first: Timothy

last: Hughes

first: William

last: Stanford


title: SAGE:15:Sau3A:Mus musculus

status: Public on Apr 24 2007

last: GEO

technology: SAGE Sau3A

description: This is a virtual platform for SAGE libraries generated for Mus musculus using Sau3A as an anchor enzyme.

distribution: virtual


title: GFNChile_Peach_0.9K_v1.0

city: Santiago

first: Mauricio

last: Gonzalez

state: Region Metropolitana

address: Santa Rosa 11610

country: Chile

organism: Prunus persica

manufacturer: Chilean Functional Genomics in Prunus persica Consortium: Universidad de Chile, Instituto de Investigaciones Agropecuarias, Asociacion de Exportadores, Fundacion para el Desarrollo Fruticola and Fundacion Chile. Supported by FDI-CORFO (FDI G02 P1001)

last: González-Agüero

first: Leonardo

last: Pavez

first: Freddy

last: Ibáñez

first: Igor

last: Pacheco

first: Reinaldo

last: Campos-Vargas

first: Lee

last: Meisel

first: Ariel

last: Orellana

first: Julio

last: Retamales

first: Herman

last: Silva

last: González

first: Verónica

last: Cambiazo

manufacture_protocol: The source of the spotted cDNA was a cv. Loring ripe peach fruit (Prunus persica L. Batsch) cDNA library. The library was constructed in the pBluescript II SK(-) vector and acquired in Clemson University Genomics Institute. A total of 847 cDNAs were selected from this library based on the functional properties assigned to their encoded proteins using an in-house gene-ranking method. This analysis involved sequence homology search using tBLASTx of 3,843 non-redundant sequenced clones from the cDNA library against the Arabidopsis CDS database to generate a list of orthologs (e-value <1E-10, identity > 70% over a minimum of 50 aa) which were further analyzed by manual searches in the annotated database, gene ontology terms, and information obtained by literature. Sequence management was performed with database tools developed in our laboratory. Domain-based analyses used SMART and Interpro. Signal peptide prediction and subcellular localization used SignalP and TargetP v1.01 software. Selected EST clones were picked from freezer stocks and grown overnight in 96 well plates in 200 µl LB plus 50 mµ/mL of ampicillin. Overnight cultures were used directly as templates in PCR reactions containing 10X buffer, 0.25 mM each of dATP, dCTP, dGTP, and dTTP, 2.5 units of Taq DNA polymerase and 0.2 µM of T3 and T7 forward/reverse primers. Aliquots (5 µl) of each reaction were loaded onto a 1.0% (w/v) agarose gel to determine product quality. 20 µL of PCR products were arrayed in 96-well plates and mixed with an equal volume of 50% of dimethyl sulfoxide. PCR products (70 ng) were single spotted onto nylon membranes (10 x 7 cm) Immobilon NY+ (Millipore) using an 8 pins print-head (Arraylt model SSP015) and the arraying robot Versarray Chip Writer Compact (BioRad). The membranes were treated and the cDNA was fixed to the membrane by UV-crosslinking using a Ultraviolet Crosslinker CL-1000 (UVP). In addition to the selected ESTs, the following controls were spotted onto membranes: (1) a fragment of the vector pBluescript II obtained by amplification with the T3 and T7 universal primers; (2) several aliquots of DMSO 50%; (3) PpExpansin (GeneBank: AB029083), a gene expressed in peach and (4) cDNA from a B. subtilis gene dap (obtained from ATCC; number 87486) used as spike mRNA to normalize between membranes.


title: ISB yeast ORF 3265-4161

first: Jennifer

last: Smith

first: John

last: Aitchison

first: Bruz

last: Marzolf


title: FilgenArray miRNA384

city: Nagoya

first: Manabu

last: Harada

state: aichi

address: 15-1 Nakanoshima, Ohdaka-cho, Midori-ku

department: Biosciences

country: Japan

institute: Filgen,Inc.

organism:
Homo sapiens
Mus musculus
Rattus norvegicus

description:
probe set: mirVana miRNA Probe Set_1564V1
Manufactured using SCHOTT’s Nexterion Slide E Technology
Surface type: non-adsorptive
Attachment: covalent
The Filgen microRNA MicroArrays are a Filgen product that is manufactured by Filgen and incorporates the Nexterion Slide E

manufacture_protocol:
Nexterion Slide E is designed to covalently immobilize amine-modified DNA for microarrays.  They are easy to use and are compatible with most available arraying and scanning instruments.  This slide has a very low variation in thickness and an ultra-flat surface, and an extremely low inhiherent fluorescence.  The epoxy surface coating allows for the efficient covalent and directed binding of molecules.  Oligonuclentides react with the epoxy coated glass surface to form a covalent bond.
Technical specifications:
Slide dimensions: 25.0 × 76.5 × 1.0 mm
1 × Printing buffer: 3 x SSC


title: GLI1 and GLI2 target genes

status: Public on May 25 2005

city: Salzburg

first: Veronika

last: Sander

address: Hellbrunnerstrasse 34

department: Molecular Biology

country: Austria

institute: University of Salzburg

description:
Human Drug Target clone set (Incyte), PCR-amplified, in-house spotted on nylon filters (MicroGridII, BioRobotics, UK), hybridised with total RNA prepared from tetracycline-inducible HaCaT cells expressing GLI1 or GLI2
Keywords = GLI target genes

first: Thomas

last: Eichberger

first: Gerhard

last: Regl

first: Fritz

last: Aberger

first: Anna-Maria

last: Frischauf


title: UMC_VET Canis familiaris 21K cDNA array

status: Public on Apr 25 2007

city: Utrecht

first: Nagesha

middle: A S

last: Rao

state: Utrecht

department: Veterinary sciences

institute: Utrecht University

organism: Canis lupus familiaris

description: Slide Dimensions (LxWxH):  	 25.3x75.5x1.1 mm

manufacturer: Genomics Laboratory, UMC Utrecht,Stratenum, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands

first: Monique

middle: E

last: van Wolferen

first: René

last: van den Ham

first: Dik

last: van Leenen

first: Marian

last: Groot Koerkamp

first: Frank

last: Holstege

first: Jan

last: Mol

manufacture_protocol: A canine specific collection of 20,160 non-redundant clones of 3’-UTR cDNA fragments inserted in pBluescript II KS and cloned into E Coli DH10B were purchased from Lion Bioscience GmbH (Heidelberg, Germany). Individual clones were grown overnight in LB supplemented with 50 µg/ml ampicillin and heated at 950C for 10 minutes. Four µl of bacterial culture was subsequently used for PCR amplification of the clone inserts using plasmid specific primers sense 5’-AGCGTGGTCGCGGCCGAGGT and antisense 5’-TCGAGCGGCCGCCCGGGCAGGT and 35 cycles of denaturation (940C, 30 sec), annealing (660C, 30 sec) and extension (720C, 1 min) using standard PCR protocols. Hundred µl PCRs were typically performed in triplicate for each clone in order to obtain enough PCR product for microarray production. Each PCR product was checked for contaminations using gel electrophoresis, and flagged if no or more than one PCR product was detected. PCR products were purified using 96-well MultiScreen plates with FB filters according to the manufacturers protocol (Millipore, Amsterdam, The Netherlands). After measurement of the concentration PCR products were air dried and reconstituted to a final concentration of 150 ng/µl in print buffer (150 mM phosphate buffer pH 8.5), and 10µl of each product was transferred to 384 well plates. PCR products that did not reach the desired concentration were flagged. PCR products were spotted on UltraGAPS slides (Corning) using the MicroGRID II arrayer (Genomic Solutions, Huntingdon, UK), air dried and immobilized by UV cross linking using a Stratalinker2400 (Stratagene, La Jolla, CA) for 10 min at 2400 mJ.  Microarrays were stored at room temperature in desiccators until needed.


title: Stillman Lab CrabChip01

status: Public on Apr 16 2007

city: Tiburon

first: Jonathon

last: Stillman

address: 3152 Paradise Drive

department: Romberg Tiburon Center

institute: San Francisco State University

organism: Petrolisthes cinctipes

description: Spotted PCR products of cloned cDNAs

first: Kristen

middle: S

last: Teranishi

manufacture_protocol: Spotted at UCSF Core for Genomics and Proteomics


title: miRNA expression in Human Uterine Leiomyomata

status: Public on Apr 15 2007

city: Newark

first: Tongsheng

last: Wang

state: NJ

address: 225 Warren Street

department: Center For Applied Genomics

institute: Public Health Research Institute

manufacture_protocol: DNA oligonucleotide probes from the mirVanaTM miRNA probe set (Ambion, Austin, TX) were used to print custom microarrays.  The miRNA microarrays consist of 206 oligos representing 206 well-characterized miRNAs, each oligo was spotted in triplicate on the chip. Probes were resuspended in 3X SSC at 50uM concentration and spotted on Nexterion Slide E (SCHOTT North America Inc. Louisville, KY) at 50% humidity using an OmniGrid100 arrayer (Genomic Solutions). The printed slides were blocked in a solution containing 100mM ethanolamine, 1M Tris (pH 9.0), and 0.1% SDS for 15 min at 50 ºC, and then thoroughly rinsed with water and spun dry.


title: MPIMG Homo sapiens 44K ArrayCGH4

status: Public on Apr 23 2007

city: Berlin

first: Fikret

last: Erdogan

address: Ihnestr. 73

country: Germany

institute: Max-Planck-Institute

manufacture_protocol: The submegabase resolution whole genome tiling path BAC array consists of the human 32k BAC Re-Array Set* (BACPAC Resources Center, DNA kindly provided by Nigel Carter, Wellcome Trust Sanger Centre) and a set if subtelomeric clones (assembled by members of the COSTB19 Action: Molecular cytogenetics of solid tumours). Protocols for the isolation of DNA (1 Mb Sanger set and subtelomeric set) and the amplification of insert DNA by linker adapter PCR are described in detail at the Molecular Cytogenetics website of MPG. Amplicons were ethanol precipitated, dissolved in 3XSSC/1,5M betaine and spotted on epoxy-coated slides (NUNC, Wiesbaden, FRG) by means of a modified Qarray (originally from Genetix, new Milton, U.K.) and Pointech (Gibbon,MN) Tungston PTL 2500 slit pins. Each BAC is represented by a single spot on the array. * due to recurrent abnormal ratios in a series of control hybridisation encompassing 30 tumors, clones from plate HGP DNA RP C 12_2 were excluded from further analysis.


title: Human X-cDNA Array

city: Baltimore

first: tao

last: wang

state: MD

address: 733 N. Broadway, BRB513

institute: Johns Hopkins University

first: Tao


title: Calame/IVT Homo sapiens 15K lymphochip

status: Public on Apr 03 2007

first: Kathryn

last: Calame

address: 701 W.168th Street, HHSC 1204

institute: Columbia University

first: Tracy

last: Kuo

first: Arthur

last: Shaffer

first: Joseph

last: Haddad

first: Yong-Sung

last: Choi

first: Louis

middle: M

last: Staudt

manufacture_protocol: See reference Alizadeh et al.Nature. 2000 Feb 3;403(6769):503-11. The lymphochip: a specialized cDNA microarray for the genomic-scale analysis of gene expression in normal and malignant lymphocytes. Cold Spring Harb Symp Quant Biol. 64, 71-8 (1999).


title: HortResearch_Arabidopsis_27K_Operon long oligo set_V1.0

city: Dunedin

first: Robert

last: Day

state: Otago

address: Cumberland Street

department: Biochemistry

country: New Zealand

institute: University of Otago

organism: Arabidopsis thaliana

first: Bart

last: Jansen

manufacture_protocol: Robot spotted, 32 pin grids each 29x29 elements. Total of 26912 elements based on Operon Arabidopsis V1.0 long oligo set plus additional controls.


title: Swegene Mus musculus 1.7K array_Operon array-ready and custom oligos

city: Tromsoe

first: Svetlana

last: Zykova

address: Breivika

department: Medical Faculty

institute: University of Tromsoe

manufacturer: Swegene Microarray Core Facility, Lund, Sweden

first: Johan

last: Staaf

first: Per

last: Lindahl

first: Ulf

manufacture_protocol: Spotted oligonucleotide array, using Operon array-ready 2.0 and custom oligos.


title: ISB yeast ORF 4866-5708

first: Jennifer

last: Smith

middle: J

first: John

last: Aitchison

first: Bruz

last: Marzolf


title: E coli O157 array virulence SOS

city: Lubbock

first: Scot

last: Dowd

address: 4321 Marsha Sharp Fwy

department: MBRI

institute: MBRI

organism: Escherichia coli

description: epoxide

manufacture_protocol: Using the transcriptome of E. coli O157:H7 EDL933 an oligonucleotide microarray, made up of 43-45 mers, was designed.The genes on the O157 array were selected based upon their being associated with virulence or with regulation of virulence genes.  Included in this new version are genes associated with pO157, SOS response, and BP933W. Specifications of oligos were based upon various design characteristics such as temperature of melting, 3’ location, specificity, lack of repeat nucleotides, etc. [32]. Oligos were synthesized and normalized in concentration by Integrated DNA Technologies Inc. (Coralville, IA). Oligos were resuspended in Epoxide Slide Spotting Solution and printed onto Epoxide Coated Slides (Corning Inc., Corning, NY) using a GeneMachine’s Omnigrid Accent microarray printer (Genomic Solutions Ann Arbor, MI).


title: Wistar Human HA0503 Array

first: Louise

last: Showe

address: 3601 Spruce St

institute: The Wistar Institute

description: cDNA spot array on nylon membrane.

first: Michael

first: Wenhwai

last: Horng


title: UT Austin Iyer yeast 6.4k ORF-only cDNA platform

status: Public on Apr 01 2007

city: Austin

first: Vishy

last: Iyer

address: 2500 Speedway Dr. MBB 3.212

institute: University of Texas at Austin

manufacture_protocol: Please refer to the Stanford Functional Genomics Facility website for all manufacture details.


title: Eurogentec S. pombe ORF array (E020F)

status: Public on Mar 16 2007

city: Stockholm

first: Karl

last: Ekwall

address: Alfred Nobels Alle 7

institute: Karolinska Inst

last: Opel

first: David

last: Lando

first: Carolina

last: Bonilla

first: Sarah

last: C. Trewick

first: Abdelhalim

last: Boukaba

first: Julian

last: Walfridsson

first: James

last: Cauwood

first: Petra

last: J.H. Werler

first: Natasha

last: Murzina

first: Antony

last: M. Carr

first: Tony

last: Kouzarides

first: Robin

last: C. Allshire

first: Ernest

last: D. Laue

manufacture_protocol:
Primary PCR: The PCR reactions are performed using tagged genome specific primers in the presence of genomic DNA (provided by your laboratory). We normally achieve a success rate of 93 % of PCR reactions. For the failed PCR reactions we perform three different protocols of PCR conditions, which include different MgCl2 concentrations and different Taq polymerase. We should normally get an additional 2 % working and reach a success rate of 95 % in total. We check 5 % of the total oligos by Mass Spectrometry and will re-synthesize maximum 2 % of the oligos based on a newly designed primer. We will perform the PCR reactions with the new designed oligonucleotides with the standard PCR protocol. Following this process we reach usually 97 % success rate.
Secondary PCR: amplifications will be performed using a single pair of primers annealing to tag sequences introduced on primary PCR primers. These PCR amplifications will also be controlled by agarose gel electrophoresis and repeated if required reaching a minimum of 95 % success rate. The final PCR products will contain an amino group at their 5' end to promote the attachment of the predicted gene coding DNA strand to the treated glass slides.


title: Streptococcus pneumoniae TIGR4/R6 v5 GAF

status: Public on Jan 26 2007

city: Groningen

first: Anne

last: Jong

address: Nijenborgh 7

institute: university of Groningen

organism: Streptococcus pneumoniae

manufacturer: Department of Molecular Genetics, university of Groningen, the Netherlands

first: Jetta

last: Bijlsma

first: Peter

last: Burghout

first: Tomas

last: Kloosterman

first: Hester

last: Bootsma

last: de Jong

last: Hermans

first: Oscar

last: Kuipers

manufacture_protocol: See Van Hijum et al, 2005, PMID: 15907200 and Hendriksen et al, 2006, PMID: 17085554


title: NCMLS Homo sapiens 1.3K TBP ChIP-cloning array

status: Public on Jan 17 2007

city: Nijmegen

first: S

last: Denissov

address: Geert Grooteplein 26

institute: NCMLS

manufacture_protocol: Inserts from the clones obtained in TBP ChIP-cloning procedure were PCR-amplified, purified and used for sequencing and printing on glass slides. Every target was printed 6 times in different parts of the slide to ensure robustness of the microarray data


title: MPG_é_C.pneumoniae_allfeatures

first: André

middle: Paul

last: Mäurer

state: Berlin

address: Charitéplatz 1

institute: Max Planck Institute for Infection Biology

organism: Chlamydia pneumoniae

manufacturer: MWG/MPG(Max Planck Insitute for Infection Biology)

first: Hans

last: Mollenkopf

first: Agnes

last: Szczepek

first: Thomas

middle: F

last: Meyer

manufacture_protocol: C. pneumoniae sqecific oligos were designed and blasted against human genome. Oligos were spotted on the array. The specifity of the oligos was approved experimentally using labeled cDNA derived from human RNA.


title: INRA-INSA Rat Operon V3 oligo array

status: Public on Mar 13 2007

city: Toulouse

first: Françoise

last: Guéraud

address: 180, Chemin de Tounefeuille BP3, Cedex 9

department: UMR1089 INRA-ENVT

country: France

institute: Institut National de la Recherche Agronomique (INRA)

description: The Rat_V3 array-ready oligo set (AROS) is based in the ENSEMBL rat v19.3b.2 database. This geneliste provides annotation based on the ENSEMBL rat v19.3b.1 database (http://ensemble.org).

first: Adriana

last: Marquez

first: Veronique

last: Le Berre

first: Jean

last: François

first: Alain

last: Paris

manufacture_protocol: Rat oligos (set_V3 Operon, 73 plates/well) were loaded at 300 pmol/well. Oligos were resuspenden in 20 µl 3X SSC buffer (final concentration 20 µM). Plates were shaken and frozen. Prior to spotting, plates are defrozen one hour at room temperature and centrifuged. Spotting was performed as single spot on Ultra GAPS glass slides (CORNING) using Qarray mini robot (GENETIX). Atmosphere moisture was set at 50% and plates were cooled to 15ºC to prevent drying. Spotting design was as follow: 48 pins therfore 48 grids, each grid contains 24X25 spots


title: Wistar Human HA05 Array

status: Public on Jul 12 2006

city: Philadelphia

first: Louise

middle: C

last: Showe

organism: Homo sapiens

description: cDNA spot array on nylon membrane.

first: Michael

first: Wenhwai

last: Horng


title: ISB Halobacterium Whole Genome SpanKit Spotted Microarray v1.3

city: Seattle

first: Bruz

last: Marzolf

institute: Institute for Systems Biology

organism: Halobacterium salinarum NRC-1

first: Marc

middle: T

last: Facciotti

first: Nitin

last: Baliga

manufacture_protocol:
MIAME platform type: spotted
MIAME availability: Arrays printed at ISB can be purchased, contact bmarzolf@systemsbiology.org for information.
Standard Operating Procedures for the Virtek(ESI) Spotter
*** Always remember that spotter pins are extremely fragile at the tips ***
To make spotter source plates:
1.	Total volume should be 4-8 ul
2.	"Usually, DNA should be in 50% DMSO.  This can be done most accurately be putting an appropriate amount of source material in spotting plates, then drying down with the speedvac and resuspending in the final volume of 3X SSC, 1.5M betaine."
Things not to do:
1.	Move slides after printing starts ? just touching a slide can shift it and other slides enough to ruin the grid geometry.
2.	Use air to blow dust off of slides - you could move the slides
3.	Press the RED EMERGENCY button if it's not a emergency- this will turn off the spotter controller and abort the entire run
4.	"Use PCR products that have been purified, then dried down and resuspended in a very small volume, this is known to cause erratic surface tension and cause spots to run together."
When starting a new print run:
1.	Allow blank slides to sit at 65% humidity for 24 hours
2.	Renew sticky mat if it's dirty
3.	Fill humidifier and turn on
4.	"Pull water out of water bath, wipe out bath and fill reservoir to get bath back to correct level- IT IS EXTREMELY IMPORTANT NOT TO OVERFILL THE WATERBATH OR SONICATOR BATH, WATER SHOULD BE JUST ABOVE THE POINT WHERE CUT RUNNING UP THE PIN ENDS"
5.	Place slides on the deck with label on the right-hand side.  Secure slides by using spacer glass slides held in place with the magnets at the end of the platform.
6.	"Measure total X and Y distances spanned by slides, enter sizes under Calibration for Arraying->Slides"
7.	"Print one ""dip"" with no source plate to ensure that pins are hitting all slides in the same plate (slight errors in Y direction especially can really throw off grid locations.)"
8.	Use the metal brace and spacer slides held by magnets to stabilize and line up the slides
9.	Stabilize humidity at approximately 65%
10.	Put blotting slide in place
To print slides:
1.	Spin down 4 source plates at 2000 rpm for one minute
2.	Put three remaining source plates (in order) at 4 C
3.	"Check blotting slide for blocked pins, change slide. Remove clogged/sticking pins and try to remedy by cleaning pin and/or printhead hole.  If a pin isn't spotting on the blotter, it may just not be contacting the blotter.see if it's been spotting on actual slides."
4.	Change water bath water at beginning of run.
5.	Check level of water reservoir and sonicator bath- add water if necessary
6.	Check humidity
7.	Double-check orientation and number of source plate
8.	Press operator button
9.	Re-seal old plate and put in cold room
10.	Set timer
11.	"Check old plate out, new plate in on printing checklist"
When finished printing for the day:
1.	Seal all plates and place in cold room
2.	Turn off  Spotter light
3.	Turn monitor off
4.	Leave computer ON
When finished with entire printing:
1.	Allow spotter humidity to slowly drop and slides dry out.
2.	Remove slides and place into plastic Telechem slide boxes.
3.	Turn off Spotter light
4.	Turn off fan/humidifier
5.	Place boxes in desiccator at least 12 hours.
6.	"Blow off slides in boxes, then bake at 85 C for 2 hours."
7.	"Etch numbers and corners on slides (corners on back side), blow dry and place in desiccator ready for prehyb."
Things to do periodically:
1.	Just keep an eye on the slides themselves to see if the grids are being formed as expected
To clean a clogged pin:
1.	Remove pin by pulling out from top with a small forceps.
2.	"Dip in water and blow dry with air tip, repeatedly if necessary."
3.	Make sure water reservoir is sufficiently full - low water often causes clogging.
Random experiments that we have tried and their results:
1.	Effect of Temperature and Humidity on Spot Size
Printed H-70 in 3X SSC onto Telechem Amine slides.  Temperatures chosen were 18 C, 20 C, 22 C, and humidities of 40%, 50%, 60% resulting in 9 different conditions.  Hybridization was with labeled PCR primer for 3 hours.
Results: Differences were seen in specific grids, but grid-grid variability was too high to establish a conclusive pattern.  Spots generally were most intense in a 90-100 micron diameter, then had a lighter "halo" that could extend to 130-150 microns.  It appeared that "Halo" size was often smallest at 18 C, 130-140 microns.  We decided to print at ambient temperature, generally 18-20 C.
2.	Effect of Volume and Humidity on Maximum Spots per Dip
Pooled 96 wells of purified PCR product and added SSC for a final concentration of 3X.  Then set up a plate so that 8 pins would print 1ul, another 8 would print 2ul, 4ul, 6ul, 8ul and 10ul.  This 48-pin dip was then printed 25 spots in the Y direction, across 10 plain glass slides for a total of 250 spots.  This print was done with increasing indent at 75%, 70%, 65%, 60% and 55% humidity.
Results: At 1-2ul, pins ran out of juice at 25-50 spots at all humidities.  At 4ul, 200-225 spots could be printed at 65% or higher humidity.  With 6ul or more, 250 spots could be printed at 65% or higher humidity.  Based upon this, we chose 65% humidity for all printing.


title: Medicago truncatula 1K array

status: Public on Oct 14 2005

city: St Paul

first: Kathryn

last: VandenBosch

state: MN

address: Room 250 BioSci 6022 1445 Gortner Ave

department: Plant Biology

institute: University of Minnesota

organism: Medicago truncatula

description: The cDNA set used for preparing this array contained a total of 1,152 clones (kiloclone set) selected by sequence analysis of M. truncatula ESTs.  Each clone is spotted in triplicate. The array contains clones from 15 M. truncatula cDNA libraries that had been 5’-end sequenced at the Institute for Genomic Research (TIGR). These libraries were generated from diverse developmental stages with emphasis on vegetative organs interacting with microbes and comparable uninfected stages: seedling roots, roots inoculated with Sinorhizobium meliloti, mature nodules and senescent nodules, mycorrhizal roots, phosphate-starved roots, Phytophthora medicaginis-infected roots, glucan-elicited roots, oligogalacturonide-elicited roots, Colletotrichum trifolii-infected leaves and cotyledons, and healthy leaves and cotyledons. The set also included organ-specific controls, positive controls (genes known to be expressed) for microbial responses, and genes with putative function as cell cycle/cell death regulators, regulators of cell organization and morphogenesis, transcription factors, metabolic enzymes as well as several genes with unknown function or no hits in GenBank.

manufacturer: University of Minnesota, VandenBosch lab

first: Gabriella

last: Endre

first: Silvia

last: Peñuela

first: Dariush

last: Danesh

first: Deborah

last: Samac

manufacture_protocol:
arrayer hardware -
Gene Machine OmniGrid array spotter


title: Wistar Human HA0502 Array

description: cDNA spot array on nylon membrane.


title: Medicago truncatula 6K array

description:
Clones selected for the array were obtained from a wide variety of cDNA libraries that were previously utilized for Expressed Sequence Tag (EST) sequencing, and were
obtained from the University of Minnesota and the Noble Foundation. We utilized the
publicly available tentative consensus sequences (TCs) from The Institute of Genomics
Research's Medicago Gene Index (http://www.tigr.org/tdb/mtgi/, Quackenbush et al.,
2001), and Minnesota Contigs (MNCs) from the Center for Computational Genomics and
Bioinformatics of the University of Minnesota (Lamblin et al., 2003) to select 6,144
clones to be included on the cDNA microarray. All selected clones represented the 5'-
most clone in a contig with maximal base call identity to the contig consensus. Clones selected for the array included those from the same contig as those present on the kiloclone array (Penuela et al., in preparation), clones from contigs that were verified to be free of chimerism and assembled consistently in both the TC and the corresponding MNC, and clones from the same contig as those present in a sample long oligo set marketed by Operon, to enable comparisons with experiments using that data.
The project was supported by the NSF Plant Genome Project (award #0110206)

first: Gabrielle

last: Penuela

first: Kevin

last: Silverstien

first: Dasharath

last: Lohar

first: Natasha

last: Sharopova

first: Christopher

last: Town

manufacture_protocol:
arrayer hardware-
Gene Machine OmniGrid array spotter


title: Wistar Human HA04 Array

description: cDNA spot array on nylon membrane.


title: DIAS_PIG_27K2_v2

status: Public on May 23 2006

city: Tjele

first: Jakob

last: Hedegaard

address: PO-box 50

department: Department of Genetics and Biotechnology

institute: Aarhus University, Faculty of Agricultural Sciences

organism: Sus scrofa

technology: mixed spotted oligonucleotide/cDNA

manufacturer: Danish Institute of Agricultural Sciences

first: Christian

last: Bendixen

first: Per

last: Horn

first: Lene

middle: N

last: Conley

first: Henrik

last: Hornshøj

first: Frank

last: Panitz

manufacture_protocol:
The DIAS_PIG_27K2_v2 array consists of 27.648 features: 5.375 PCR products amplified from cDNA clones, 985 control features and 552 oligonucleotides spotted as quadruplicates. The array consists of 4 x 12 sub arrays each of 24 x 24 spots. The four replicated spots were spotted pair wise on different locations on the slide. Practically, the 6.912 elements were first spotted twice in 2 x 12 sub arrays (sub array-column 3 and 4). The source plates were then rotated 180 ° and the final 2 x 12 sub arrays (sub array-column 1 and 2) were spotted.
The cDNA clones used as template were selected from the cDNA libraries generated in the Sino-Danish Pig Genome Sequencing Consortium (Gorodkin et al.: Porcine transcriptome analysis based on 97 non-normalized cDNA libraries and assembly of 1,021,891 ESTs. Genome Biol 2007, 8:R45). The cDNA clones were selected by blasting the human sequences of genes of interest for similarity (P ≤ 10-8) to members of the porcine EST selection at DIAS. Furthermore, a number of clones were selected from lung, liver and muscle Longissimus dorsii cDNA libraries to represent major clusters of EST sequences.
The control features consists of dilution series of selected clones and oligonucleotides, genomic DNA from pig and salmon, sheared pig and salmon genomic DNA, ds cDNA from pig lung and muscle, different porcine C0t DNA fractions and blanks.
The 552 oligonucleotides (70-mer) were designed from the sequence of p450 related genes using the SEPON software (Hornshøj et al, “SEPON, a Selection and Evaluation Pipeline for OligoNucleotides based on ESTs with a non-target Tm algorithm for reducing cross-hybridization in microarray gene expression experiments”, Bioinformatics. 2004 Feb 12;20(3):428-9).
The arrays were spotted on UltraGAPS slides (Corning Incorporated) at 20 C/55 % RH using an SDDC-2 MicroArrayer (BioRad) with 24 SMP3 stealth pins (Telechem International) with DNA resuspended in Micro Spotting Solution (Telechem International). Slides were dried and UV cross-linked (300 mJ) and stored in a vacuum desiccator until use.


title: WUSTL Streptococcus pyogenes 3K

city: St. Louis

first: Michael

middle: G

last: Caparon

state: MO

address: 660 S. Euclid Ave.

department: Dept. of Molecular Microbiology

institute: Washington University School of Medicine

organism: Streptococcus pyogenes

description: An antisense oligonucleotide array based on the published genome of S. pyogenes SF370 (AE004092.1  GI:14286347) was made at the Microarray Core Facility in the Genome-Sequencing Center at Washington University (GSC). A single 60–80 mer oligonucleotide was designed to hybridize to the putative transcripts from each annotated open reading frame using 'TOP' software that was created at the GSC. To reduce complexity, probes for rRNA, tRNA and prophage-associated genes were not included, which resulted in an array consisting of probes for 1517 genes out of a total of 1752 predicted open reading frames in the SF370 genome.

first: Audrey

last: Brenot

first: Seth

last: Crosby

manufacture_protocol: Oligos were synthesized using standard methods by Illumina (San Diego, CA). These oligonucleotides were dissolved at a concentration of 50 μM in 3X SSC with 0.75M betaine and were printed in duplicate on MWG Epoxy slides by a locally constructed linear servo arrayer (after the DeRisi model, see the DeRisi lab web page at UCSF).


title: 454 sequencing of small RNAs from mouse

status: Public on Apr 20 2007

city: Cold spring Harbor

first: ravi

last: Sachidanandam

address: 1 bungtown road

institute: Cold Spring Harbor Laboratory

organism: Mus musculus

technology: other

first: Alexei

middle: A

last: Aravin

first: Ravi

first: Angelique

last: Girard

first: Katalin

last: Fejes-Toth

first: Gregory

last: Hannon