title: Atlas Rat Stress Array (Cat. #7735-1)

status: Public on Oct 01 2002

city: Palo Alto

last: BD Biosciences Clontech

state: CA

address: 1020 East Meadow Circle

country: USA

institute: BD Biosciences Clontech

organism: Rattus norvegicus

technology: spotted DNA/cDNA

description: Nylon, rat cDNA array designed to study stress.  The cDNA fragments (200-600 bp) are PCR-amplified using gene-specific primers, and are selected so that they lack repetitive elements and have minimal homology with other genes on the array.

distribution: commercial

manufacture_protocol: see manufacturer's web site


title: GE Codelink Human Uniset I, II, and 20K

status: Public on Jul 22 2005

city: Charlestown

first: Dimitri

last: Krainc

state: MA

address: Building 114, 16th St.

department: Neurology

institute: MassGeneral Institute for Neurodegeneration

organism: Homo sapiens

technology: spotted oligonucleotide

description: GE/Amersham Codelink Human Uniset I, II, and 20K oligonucleotide arrays

distribution: custom-commercial


title: Affymetrix GeneChip Human Genome U133 Plus 2.0 Array [CDF: Hs_ENTREZG_10]

status: Public on Apr 29 2008

city: Wageningen

first: Guido

last: Hooiveld

address: HELIX, Stippeneng 4

department: Div. Human Nutrition & Health

country: Netherlands

institute: Wageningen University

technology: in situ oligonucleotide

description:
CDF file was created by Microarray Lab at the Molecular and Behavioral Neuroscience Institute at the University of Michigan.
Oligonucleotide probes on GeneChips were reorganized based on the latest genome and transcriptome information. In this particular case the Entrez Gene database (build: Sept 11, 2006) was used as source.
For the CDF file based on Entrez Gene:
A probe must hit only one genomic location;
Probes that can be mapped to the same target sequence in the correct direction are grouped together in the same probe set;
Each probe set must contain at least three oligonucleotide probes and probes in a set are ordered according to their location in the corresponding exon.
For detailed description please see the paper and website.

manufacture_protocol: This array is identical to GPL570 but a custom, remapped CDF environment is used to extract data. This is version 10 of the remapped CDF. The CDF for various platforms can be found at the link below.


title: NIH/NIA_mouse_17k

status: Public on Sep 01 2006

city: Bethesda

first: Christopher

middle: Dennis

last: Toscano

state: MD

address: 9 Memorial Drive Room 1S126

department: National Institute on Aging

institute: National Institutes of Health

organism: Mus musculus

distribution: non-commercial

middle: D

first: Vinaykumar

middle: V

last: Prabhu

first: Kevin

middle: G

last: Becker

first: Francesca

last: Bosetti

manufacture_protocol:
Protocols are found at
http://www.grc.nia.nih.gov/branches/rrb/dna/dna.htm


title: Fine-Scale Mapping and Sequencing of Structural Variation from Eight Human Genomes

status: Public on Apr 30 2008

city: Seattle

first: Jeffrey

middle: M

last: Kidd

state: WA

address: 1705 NE Pacific St

department: Department of Genome Sciences

institute: University of Washington

first: Gregory

last: Cooper

first: William

middle: F

last: Donahue

first: Hillary

middle: S

last: Hayden

first: Nick

last: Sampas

first: Tina

last: Graves

first: Nancy

last: Hansen

first: Brian

last: Teague

first: Can

last: Alkan

last: Antonacci

first: Eric

last: Haugen

first: Troy

last: Zerr

last: Yamada

first: Peter

last: Tsang

first: Tera

middle: L

last: Newman

first: Eray

last: Tuzun

first: Ze

last: Cheng

first: Heather

last: Ebling

first: Nadeem

last: Tusneem

first: Robert

last: David

first: WIll

last: Gillett

first: Karen

middle: A

last: Phelps

first: Molly

last: Weaver

first: David

last: Saranga

first: Adrianne

last: Brand

first: Wei

last: Tao

first: Erik

last: Gustafson

last: McKernan

first: Lin

last: Chen

first: Maika

last: Malig

first: Joshua

last: Smith

last: Korn

first: Steven

last: McCarroll

last: Altshuler

first: Daniel

last: Peiffer

first: Michael

last: Dorschner

first: John

last: Stamatoyannopoulos

last: Schwartz

manufacture_protocol: Manufactured by NimbleGen according to manufacturer's protocol.


title: SHCZ

status: Public on Apr 15 2004

city: Stanford

first: Jonathan

last: Pollack

address: 269 Campus Drive, CCSR3245A

department: Pathology

institute: Stanford University

description:
Tip Configuration: Standard 48 tip
Columns per Sector: 30
Rows per Sector: 30
Column Spacing: 146
Row Spacing: 146


title: Atlas Rat 1.2 Array (Cat. #7854-1)

last: BD Biosciences Clontech

description: Nylon, broad-coverage rodent cDNA array.  The cDNA fragments (200-600 bp) are PCR-amplified using gene-specific primers, and are selected so that they lack repetitive elements and have minimal homology with other genes on the array.


title: Atlas Mouse 1.2 Array (Cat. #7853-1)

description: Nylon, broad-coverage murine cDNA array.  The cDNA fragments (200-600 bp) are PCR-amplified using gene-specific primers, and are selected so that they lack repetitive elements and have minimal homology with other genes on the array.


title: SHCO


title: CEA_SGF:AHX

status: Public on Apr 01 2004

city: Versailles

first: Nelly

last: Godefroy

address: 45 Avenue des Etats Unis

department: Laboratoire de Genetique et de Biologie Cellulaire

country: France

institute: Universite de Versailles

description:
Chip contains 9216 features (probes and controls):
2014 PCR products from cDNA matrix using primers specific of genes involved in important biological process (apoptosis,  cell cycle, stress response).
1684 PCR products from cDNA clones from the I.M.A.G.E consortium (Research Genetics collection).
2014 PCR products from mouse cDNA clones of a substractive bank (myodistrophic versus normal muscle).
1800 PCR products from rat cDNA clones of a substractive bank (atrophied versus normal muscle).
1704 control reporters.


title: Atlas Rat 1.2 Array II (Cat. #7856-1)


title: Hannon Lab Solexa Drosophila melanogaster

city: Cold Spring Harbor

first: Julius

last: Brennecke

state: NY

address: 1 Bungtown Road

institute: Cold Spring Harbor Labs

organism: Drosophila melanogaster

technology: other

distribution: virtual


title: Atlas Human 1.2 Array II (Cat. #7852-1)

description: Nylon, broad-coverage human cDNA array.  The cDNA fragments (200-600 bp) are PCR-amplified using gene-specific primers, and are selected so that they lack repetitive elements and have minimal homology with other genes on the array.


title: Amersham Biosciences CodeLink Uniset Human I Bioarray

status: Public on Aug 08 2005

city: Pittsburgh

first: Thomas

last: Richards

state: PA

institute: University of Pittsburgh

description: CodeLink™ UniSet Human I targets approximately 10 000 of the most well-annotated human genes published in public databases, derived from the public UniGene and RefSeq databases, and sequences for design were derived from Incyte™ LifeSeq™ Gold and Foundation databases.


title: Atlas Human 1.2 Array (Cat. #7850-1)

description:
Nylon, broad-coverage human cDNA array.  The cDNA fragments (200-600 bp) are PCR-amplified using gene-specific primers, and are selected so that they lack repetitive elements and have minimal homology with other genes on the array.

Also see Cat# 634527 at http://www.clontech.com/clontech/atlas/genelists/index.shtml


title: ABI Mouse Genome Survey Microarray

status: Public on Jan 13 2006

city: Rockville

first: chunlin

last: xiao

address: 45 West Gude Drive

institute: Applied Biosystem

description: See manufacturer's web site

manufacture_protocol: See manufacturer's web site


title: Micro-CRIBI Human Oligo Array (Operon V2.0)

status: Public on Jun 06 2005

city: Padova

first: Gerolamo

last: Lanfranchi

address: Via U. Bassi, 58/B

department: CRIBI - Biotechnology Center and Biology Department

country: Italy

institute: University of Padova

description:
This set consists of 21,329 70mer oligonucleotides (Operon version 2.0) designed on Human Unigene clusters, mainly in the 3 end terminal region. Each oligonucleotide is spotted by our robotic station Biorobotics Microgrid II, in two replicates on a MICROMAX glass slide SuperChip I (Cat No MPS696) provided by PerkinElmer Life Sciences Inc. The deposition is qualitatively assessed by a scan of the spotted slide at 10 micron resolution, using a GSI Lumonics scanner and a protocol that allows the detection of spotted features through the salts derived from the dried solution in which oligonucleotides are dissolved. Microarray slides that meet the quality standards, are washed as recommended by the supplier and the glass slides are stored, ready for use, in the dark under deep vacuum. Click below to see images of the array and detailed description of the spotting geometry, with the necessary parameters for analysis.

Keywords = Oligo
Keywords = probe 70mer
Keywords = MicroCribi
Keywords = probe set 21.329

first: Stefano

last: Cagnin

first: Beniamina

last: Pacchioni

first: Caterina

last: Millino

first: Barbara

last: Celegato

first: Cristiano

last: De Pittà

first: Lucia

last: Tombolan

first: Chiara

last: Romualdi


title: Atlas Human Cancer 1.2 Array (Cat. #7851-1)

description: Nylon, human cDNA array designed to study cancer.  The cDNA fragments (200-600 bp) are PCR-amplified using gene-specific primers, and are selected so that they lack repetitive elements and have minimal homology with other genes on the array.


title: NCSU_Metallosphaera sedula_2Karray_version1

city: Raleigh

first: Kathryne

middle: Sherlock

last: Auernik

state: NC

address: Partners II, rm 3309, 849 Main Campus Drive

department: Chemical and Biomolecular Engineering

institute: North Carolina State University

organism: Metallosphaera sedula

first: Robert

last: Kelly

manufacture_protocol: A DNA microarray was designed and constructed based on at least 2258 protein-coding sequences in the M. sedula genome obtained from the Department on Energy’s Joint Genomes Institute (http://genome.ornl.gov/microbial/msed). OligoArray 2.1 (Rouillard, 2003) was used to generate at least one 60-mer oligonucleotide probe sequence for each open reading frame. The probes were synthesized (Integrated DNA Technologies, IA), re-suspended in 50% DMSO, and printed onto Ultragap microarray slides (Corning, NY) using a QArrayMini arrayer (Genetix, UK). Each probe was spotted five times onto each array to fortify statistical analysis.


title: Agilent-014928 Mouse Promoter tiling array set. Microarray 1 of 2

city: Boston

first: Peter

last: Kharchenko

address: 10 Shattuck St.

department: DBMI

institute: Harvard Medical School

manufacture_protocol: see manufacturer's web site at http://www.agilent.com/


title: AsalChip2 (NRC-IBS Aeromonas salmonicida A449)

city: Ottawa

first: John

middle: H

last: Nash

state: Ontario

address: 100 Sussex Drive

department: Institute for Biological Sciences

country: Canada

institute: National Research Council

organism: Aeromonas salmonicida subsp. salmonicida A449

description: PCR primers were designed for 4293 ORFs from the completed genomic sequence of Aeromonas salmonicida A449 which comprises 4413 ORFs on one chromosome and two large plasmids. Good amplicons of length greater than 160 nt were obtained for 4237 ORFs representing >95% coverage of ORFs. All amplicons are spotted in duplicate on the microarray and sheared total genomic DNA from A. salmonicida and salmon DNA were spotted in the corners of each subarray as controls.

first: Wendy

last: Findlay

first: Sandra

last: Sperker

first: Oksana

last: Mykytczuk

first: Laura

last: Brown

first: Michael

middle: E

last: Reith

manufacture_protocol: DNA microarrayer (Bio-Rad) equipped with a 48-pin print-heads that uses "quill" pin technology (TeleChem / ArrayIt).


title: 6240 rat cDNA array

status: Public on Dec 31 2003

city: Stockholm

first: Petra

middle: Elisabeth

last: Tollet-Egnell

address: CMM L8:01

department: Dept of Molecular Medicine

country: Sweden

institute: Karolinska Institute

description: 6240 rat cDNA array

first: Nina

last: Ståhlberg

first: Amilcar

last: Flores-Morales

first: Gunnar

last: Norstedt


title: Atlas Rat 3.8 I Microarray (Cat. #7911-1)

status: Public on Jul 08 2004

city: Tokyo

first: BD Biosciences

last: Clontech

address: 2-6-1 Nishi-Shinjyuku Sumitomo Bldg. 9F

country: Japan

description: Glass microarrays made with long (~80 bp) experimentally-tested oligonucleotides.


title: SalkGAL-Arabidopsis-IlluminaGA-epigenome - small RNA sequences

status: Public on Apr 21 2008

city: La Jolla

first: Joseph

middle: R

last: Ecker

address: 10010 North Torrey Pines Road

department: Genomic Analysis Laboratory

institute: HHMI-Salk-Institute

organism: Arabidopsis thaliana

description: Genetic Analyzer, Illumina, San Diego, CA.


title: Nsp copy number (CN) array (ds1)

status: Public on Apr 24 2008

city: Santa Clara

first: Fan

last: Shen

address: 3420 Central Expressway

institute: Affymetrix, Inc

description:
Research Array
The Nsp copy number (CN) array contains 12,339,139 oligonucleotide probes tiled onto two arrays (ds1 and ds2 arrays). Probes were selected to represent each of the 1,330,354 fragments between 200-1100bp predicted to arise after digestion of human genomic DNA with the restriction enzyme NspI.
Platform data table annotation based on genome build 35: hg17 (May 2004 build).


title: JCVI PFGRC Vibrio vulnificus 23K v1 array designed primarily based on strain CMCP6

status: Public on Apr 22 2008

first: PFGRC

last: JCVI

address: 9704 Medical Center Drive

department: PFGRC

institute: J. Craig Venter Institute

organism: Vibrio vulnificus

description: This set includes 10210 oligonucleotides, has 23232 spots distributed in 48 blocks.

manufacture_protocol: QVV, with arrayer GENETIX, machine cycles 0, pen id 0, pen cycles 0, operator 0, humidity control 0, humidity set point0.000


title: JCVI PFGRC Vibrio vulnificus 23K v1 array designed primarily based on strain YJ016


title: Nsp copy number (CN) array (ds2)


title: Sigma Genosys Panorama Human Cytokine Gene Array Version 2

status: Public on Nov 10 2002

city: The Woodlands

last: Sigma-Genosys

state: TX

address: 1442 Lake Front Circle

institute: Sigma-Genosys

description:
The Panorama Human Cytokine Gene Array ver 2 represents a comprehensive collection of cytokines, chemokines and other immunomodulatory factors, and their receptors. The array consists of 847 cDNAs printed as PCR products onto positively charged nylon membranes.  Each gene is printed at 10 ng per spot. The array contains coding sequence-specific PCR products derived from fully sequence-characterized cDNA clones. The amplified gene sequences are derived from coding sequences devoid of repetitive sequence elements (i.e. Alu repeats) that are often associated with untranslated regions of cDNAs.
This array represents an update of the original Sigma Genosys cytokine array (GPL192).


title: SHDJ

first: Jonathan

last: Pollack


title: Clontech Atlas Mouse cDNA Expression Array (Cat. #7741-1)

status: Public on Aug 27 2002

city: St. Louis

first: Jason

middle: Matthew

last: Laramie

state: MO

address: 660 South Euclid Avenue

department: Surgery

institute: Washington University

first: Perren

last: Cobb


title: Yonsei University Drosophila-6k-twin-microarray version 1

status: Public on Apr 10 2008

city: Seoul

first: Young-Joon

last: Kim

address: Yonsei Univ 134 Sinchon-dong Seodaemun-gu

country: South Korea

institute: Yonsei University

manufacture_protocol:
cDNA were prepared by PCR and cleanup, and then spotting.
Spotting was carried out with Cartesian PixSys5500 spotter.
Amine coated GAPSII (Corning 40006) slides were laid on spotter. Each purified PCR product was transferred to plate, and laid on source's place.
Then, spotter was operated, and fly twin chips were fabricated.


title: BOAC CattleOligo 13.2K + 8.7K 70-mer microarray v2

status: Public on Apr 09 2008

city: Jouy-en-Josas

first: Sylvain

last: Marthey

address: Domaine de Vilvert Bat 320

department: Animal Genetics

institute: INRA

organism: Bos taurus

description: spotted oligonucleotide

manufacture_protocol:
1-1. The oligos set used for this microarray is a mix of the 8.7k set from Operon and a 13.2K
set designed by the Illinois University and synthetised by Illumina.
1-2. Oligos are arrayed by Illumina in Greiner 384-well flat-bottom plates. Each well contains 3000 pmol of 70-mer oligo.
1-3. Oligos are arrayed by Operon in Genetix 384-well V-bottom plates. Each well contains 600 pmol of 70-mer oligo.
2. Resuspend oligos in water(20µM).
3. Incubate at room temperature during a night shaking plates to aid resuspension of DNA.
4. Deep freeze and defrost centrifuging plates at 1000 rpm for 16 minutes then 2000 rpm for 3 minutes.
5. Take 5 µl of oligos solution and put it in a new genetix polypropylène v-bottom plates(cat# X7022)
6. Add 5 µL of 1X Printing Buffer to each well and mix.
7. Centrifuge plates at 2000 rpm for 3 minutes.
8. Oligos are ready for spotting.
9. Spotting on glass slides, with 48 pins on cheapwriter of Virtek, with control of humidity (45-50%).
10. Fixation of the oligos slides with UV (600 mJoules).
11. Storage the slides in a vacuum.
12. After the print run is completed, cover oligos plates with seals and deepfreeze them at 20°C in a protected environment.


title: P. vivax exon array v1.0 using oligos designed by Z. Bozdech and  G. Hu

status: Public on Apr 12 2008

city: Barcelona

first: Hernando

last: del Portillo

state: Barcelona

address: rosello, 132 (4a)

country: Spain

institute: Barcelona Centre for International Health Research

organism: Plasmodium vivax

first: Ricardo

middle: Z

last: Vencio


title: Agilent-014929 Mouse Promoter tiling array set. Microarray 2 of 2

status: Public on Apr 29 2008

first: Peter

last: Kharchenko


title: MHP Human 1Mb-resolution CGH array

status: Public on Apr 28 2008

city: Cambridge

first: David

middle: T W

last: Jones

address: Box 231, Level 3 Lab Block, Addenbrooke's Hospital

country: United Kingdom

institute: University of Cambridge

description: The 1Mb clone set has been assembled by the Mapping Core, Map Finishing and Microarray Facility groups of the Wellcome Trust Sanger Institute, Hinxton, UK. Additional clones were also kindly supplied by the Wellcome Trust Sanger Institute. The array has 24 subarrays (6 rows by 4 columns), each subarray consists of 18 rows by 18 columns.

manufacturer: Department of Pathology, Univeristy of Cambridge

first: Koichi

last: Ichimura

first: Danita

last: Pearson

last: Carter

first: Anthony

last: Brown

first: V

middle: P

last: Collins

manufacture_protocol:
Spotted DNA array
1.BAC/PAC clones were grown in 2xTY medium in a deep-well titre plate at 37°C overnight with chloramphenicol 25µg/ml (BAC) or kanamycin 20µg/ml (PAC).
2. DNA was extracted from BAC or PAC clone using modified alkali-lysis method (microprepping).
3. Approximately 5ng of BAC/PAC DNA was PCR-amplified using three DOP-primers (Fiegler H, Genes Chromosomes Cancer 36:361-374, 2003).
4. Two microlitre of the DOP-PCR products was further amplified using 5'-Aminolink universal primer (Fiegler H, Genes Chromosomes Cancer 36:361-374, 2003).
5. Three volumes of the Amino-PCR products were mixed with one volume of 4xSpotting buffer (1M Sodium phosphate buffer, pH8.5, 0.001% Sarkosyl) to make a spotting mix.
6. The spotting mix was filtered through Corning 96 well white polystyrene filter plate with modified styrene insert (0.2µm PVDF) by centrifugation.
7. The filtered spotting mix was transferred to a 384-well plate.
8. The spotting mix was printed printed in duplicate onto a CodeLink slide (GE Healthcare) using a MicroGrid II robot (Genomic Solutions, Huntingdon, UK) in 4x4 subarrays.
9. The printed slides were incubated at room temperature for 24-72 hours in a humidified chamber.
10. The slides were treated with 1% ammonium hydroxide at room temperature for 5 min, then with 0.1% SDS at room temperature for 5min.
11. The slides were heat-denatured in a boiling water for 2 min, cooled, rinsed with water and dried.


title: JCVI PFGRC Streptococcus pneumoniae 21K v7 array designed primarily based on strain MM1 1998

organism: Streptococcus pneumoniae

description: This set includes 3942 oligonucleotides, has 21168 spots distributed in 48 blocks.

manufacture_protocol: 7QSP, with arrayer GENETIX, machine cycles 0, pen id 0, pen cycles 0, operator 0, humidity control 0, humidity set point0.000


title: JCVI PFGRC Streptococcus pneumoniae 21K v7 array designed primarily based on strain R6


title: miRNA_Array_1566V2_16UP

status: Public on Apr 11 2008

city: Austin

first: Bhavna

last: Choudhury

address: 2150 Woodward street

institute: Asuragen

organism:
Homo sapiens
Mus musculus
Rattus norvegicus

manufacture_protocol: Codelink technology


title: MERH03-S1-0003 (Rhodococcus sp. RHA1 11K)

city: Vancouver

first: Shawn

last: Anderson

address: The Prostate Centre at Vancouver General Hospital, 2660 Oak Street

department: Urological Sciences

institute: University of British Columbia

organism: Rhodococcus jostii RHA1

manufacturer: The Microarray Facility of the Prostate Centre at Vancouver General Hospital

manufacture_protocol: Oligonucleotides are consolidated into 384-well plates, lyophilized by speed-Vac, and resuspended in 3x SSC to a printing concentration of 30uM. Oligos are printed on aminosilane slides (Erie, Hudson, NH, USA) with a Biorobotics Microgrid II microarray printer (Genomic Solutions). Microspot 10K quill pins (Biorobotics) in a 48-pin tool were used to deposit <0.5 nL per spot onto the slide. The resulting microarrays have a 4 x12 subgrid layout with 392 spots per subgrid, each spot having approximate diameter and pitch of 90 µm and 190µm, respectively. A 280-bp GFP (green fluorescent protein) oligo was printed in subgrid corners to assist in placing on the grid. The slides were crosslinked in a UV Stratalinker 2400 (Stratagene) at 300mJ. Array quality was assessed by visual inspection and hybridization of representative slides from a print run by dye-labelled random 9-mer oligonucleotides. The quality control images were aquired via the ScanArray Express from Perkin Elmer at a 10 micron resolution and quantified with the Imagene 6.0 software suite.


title: JCVI PFGRC Haemophilus influenzae 11K v2 array designed primarily based on strain pF3028

city: Rockville

last: JCVI

organism: Haemophilus influenzae

description: This set includes 4574 oligonucleotides, has 11424 spots distributed in 48 blocks.

manufacture_protocol: 2QHI, with arrayer LUCIDEA, mode Normal, replicates 2, repeats 1, slide_dips 40, machine cycles 1099390, pen id 186, pen cycles -1, operator Robin/Chris, humidity control On, humidity set point65


title: Drosophila melanogaster small RNA sequence by Solexa

status: Public on Apr 23 2008

city: New York

first: Wei-Jen

last: Chung

address: 430 E 67th St

department: Developmental Biology

institute: Sloan-Kettering Institute

first: Eric

last: Lai


title: Hannon Lab Solexa Flock house virus

status: Public on Apr 30 2008

city: Cold Spring Harbor

first: Julius

last: Brennecke

address: 1 Bungtown Road

institute: Cold Spring Harbor Labs

organism: Flock House virus


title: SalkGAL-Arabidopsis-IlluminaGA-epigenome - bisulphite sequencing

first: Joseph

last: Ecker


title: Atlas Human Cardiovascular Array (Cat. #7734-1)

status: Public on Oct 18 2003

city: Palo Alto

last: BD Biosciences Clontech

address: 1020 East Meadow Circle

institute: BD Biosciences Clontech

description:
Nylon. The cDNA fragments (200-600 bp) are PCR-amplified using gene-specific primers, and are selected so that they lack repetitive elements and have minimal homology with other genes on the array.
Keywords = Cardiovascular
Keywords = Nylon filter
Keywords = cDNA
Keywords = Clontech

manufacture_protocol: see manufacturer's web site.


title: Print_1300

status: Public on Apr 08 2008

city: Stanford

first: Daniel

last: Hogan

address: 279 Campus Dr B439

institute: Stanford University

manufacturer: Stanford Functional Genomics Facility, Stanford School of Medicine

manufacture_protocol: Material is transferred from microtiter plate wells to the slide surface by an automated print head


title: JCVI PFGRC Haemophilus influenzae 11K v2 array designed primarily based on strain HK1212

first: PFGRC


title: SalkGAL-Arabidopsis-IlluminaGA-epigenome - transcriptome sequences


title: NCI/ATC Hs-ROSP-8k

status: Public on Apr 15 2008

city: Bethesda

first: Uma

middle: T

last: Shankavaram

address: 9000 Rockville Pike

department: NCI

institute: NIH

manufacturer: Advanced Technology Center Microarray Facility (NIH/NCI/CCR/ATC)

first: Sandhya

last: Kumaraswamy

first: Prakash

last: Chinnaiyan

first: Xing

last: Lu

first: Kevin

last: Camphausen

first: Philip

middle: J

last: Tofilon

manufacture_protocol: The DNA microarrays were produced by Radiation Oncology Sciences Program, NCI and contained 7680 human cDNA clones from the Research Genetics Named Genes set (Huntsville, AL). These cDNA clones were enriched for known genes. All 7680 cDNAs were spotted onto poly-l -lysine-coated slides (NCI ROSP 8K Human Array) using an Omni-Grid arrayer (GeneMachines, San Carlos, CA).


title: Eppendorf DualChip Human Cancer v1.1

city: Genova

first: PAOLA

last: SCARUFFI

state: GE

address: LARGO R. BENZI, 10

institute: "San Martino" Hospital

description:
No. of genes: 294 (including housekeeping genes)
No. of housekeeping genes:  13
No. of spots per gene: 3
Average spot size: 200 µm
Controls:  RNA spiking mixture, housekeeping genes, positive/negative hybridization controls, positive/negative detection controls
No. of spiking RNAs for normalization: 6

first: Paola

last: Scaruffi

first: Stefano

last: Moretti

first: Caterina

last: Cremonesi

last: Bonassi

first: GianPaolo

last: Tonini

manufacture_protocol:
Each slide features two identical microarrays, printed side-by-side.
Unique Xmer® probe technology features 200-400 nucleotides long sense DNA.
Each Xmer® is in silico designed and tested in real hybridization experiments.


title: Nimblegen human CIITA tiling array (HG17) design2

city: Toronto

first: Zhaodong

last: Xu

address: Mc6-424, 399 Bathurst Street

department: Genetics & Development Division

institute: TWRI, UHN

first: Rod

last: Bremner

first: Tao

last: Yu

manufacture_protocol: as descrbed in www.nimblegen.com


title: Nimblegen human CIITA tiling array (HG17) design1

description: Custom 122kb tiling array, chromosome 16:10827079-10950521 tiled from HG17; 50-mer tiling at 30 bp interval. 7151 control probes added.

manufacture_protocol: as described in www.nimblegen.com


title: U919_Human_9.5K_V1.0

status: Public on Apr 16 2008

city: Caen

first: Benoit

middle: Denis

last: Roussel

address: Bd Becquerel

department: GIP Cyceron

institute: INSERM

manufacture_protocol: SOX9 was overexpressed in SW1353 chondrosarcoma cells. RNA extracted from these cell or form those transduced with a control retrovirus were compared by microarray analysis on "Human known gene SGC oligo set array number 1" glass spotted arrays obtained from HGMP. One RNA sample was derived from each cell type and was subjected to 4 repeated hybridisations. 2 of these were dye swaps.


title: JCVI PFGRC Campylobacter jejuni 23K v1 array designed primarily based on strain NCTC_11168

last: JCVI

address: 9704 Medical Center Drive

institute: J. Craig Venter Institute

organism: Campylobacter jejuni

description: This set includes 6868 oligonucleotides, has 23232 spots distributed in 48 blocks.

manufacture_protocol: QCJ, with arrayer GENETIX, machine cycles 0, pen id 0, pen cycles 0, operator 0, humidity control 0, humidity set point0.000


title: Drosophila melanogaster deep-sequencing small RNAs

city: Worcester

first: Hervé

last: Seitz

address: 364 Plantation Street

department: Biochemistry and Molecular Pharmacology

institute: University of Massachusetts Medical School

first: Megha

last: Ghildiyal

first: Michael

middle: D

last: Horwich

first: Chengjian

last: Li

first: Tingting

last: Du

first: Soohyun

last: Lee

first: Jia

first: Ellen

last: Kittler

first: Maria

last: Zapp

first: Zhiping

last: Weng

first: Phillip

last: Zamore

manufacture_protocol:
High-throughput sequencing-by-synthesis (Genome Analyzer, Illumina, San Diego, CA, USA) was as for pyrosequencing in Seitz et al. (Curr. Biol. 18, 147 (2008)) except that RNA Ligase 2 [Rnl2(1-249)K227Q] (Addgene, Cambridge, MA, USA) was used for 3´ ligation. Linkers and primers for sequencing-by-synthesis were: 5´ adaptor, 5´-rGrUrU rCrArG rArGrU rUrCrU rArCrA rGrUrC rCrGrA rCrGrA rUrC-3´ (Dharmacon, Lafayette, CO, USA); 3´ preadenylated linkers, 5´-rAppdCdT dGdTdA dGdGdC dAdCdC dAdTdC dAdAdT ddC-3´.
After linker addition, the cDNA was synthesized using a reverse transcriptase primer corresponding to the 3´ adapter and amplified by PCR using forward (5´-dAdAdT dGdAdT dAdCdG dGdCdG dAdCdC dAdCdC dGdAdC dAdGdG dTdTdC dAdGdA dGdTdT dCdTdA dCdAdG dTdCdC dGdA -3´) and reverse (5´-dCdAdA dGdCdA dGdAdA dGdAdC dGdGdC dAdTdA dCdGdA dAdTdT dGdAdT dGdGdT dGdCdC dTdAdC dAdG-3´) primers. The PCR pool was gel purified (4% Metaphor Agarose, Cambrex, East Rutherford, NJ, USA) with Qiaex II (Qiagen, Valencia, CA, USA) then sequenced (Genome Analyzer, Illumina) according to the manufacturer’s protocol.


title: MPI_Chen_HeLa_Solexa_2007

status: Public on Apr 07 2008

city: Berlin

first: wei

last: chen

address: Ihestrasse 73

country: Germany

institute: MPI molecular genetics


title: BOAC CattleOligo 13.2K + 8.7K 70-mer microarray v1

first: Sylvain

last: Marthey

description: spotted oligonucleotide

manufacture_protocol:
1-1. The oligos set used for this microarray is a mix of the 8.7k set from Operon and a 13.2K
set designed by the Illinois University and synthetised by Illumina.
1-2. Oligos are arrayed by Illumina in Greiner 384-well flat-bottom plates. Each well contains 3000 pmol of 70-mer oligo.
1-3. Oligos are arrayed by Operon in Genetix 384-well V-bottom plates. Each well contains 600 pmol of 70-mer oligo.
2. Resuspend oligos in water(20µM).
3. Incubate at room temperature during a night shaking plates to aid resuspension of DNA.
4. Deep freeze and defrost centrifuging plates at 1000 rpm for 16 minutes then 2000 rpm for 3 minutes.
5. Take 5 µl of oligos solution and put it in a new genetix polypropylène v-bottom plates(cat# X7022)
6. Add 5 µL of 1X Printing Buffer to each well and mix.
7. Centrifuge plates at 2000 rpm for 3 minutes.
8. Oligos are ready for spotting.
9. Spotting on glass slides, with 24 pins on cheapwriter of Virtek, with control of humidity (45-50%).
10. Fixation of the oligos slides with UV (600 mJoules).
11. Storage the slides in a vacuum.
12. After the print run is completed, cover oligos plates with seals and deepfreeze them at 20°C in a protected environment.


title: NCSU_Caldicellulosiruptor saccharolyticus_3K array_version 1

city: Raleigh

first: Amy

last: VanFossen

address: 840 Main Campus Dr.  Rm3305

institute: NC State Unversity

organism: Caldicellulosiruptor saccharolyticus

manufacture_protocol: A DNA microarray was designed and constructed based on 2793 protein-coding sequences in the C. saccharolyticus genome obtained from the Department on Energy's  Joint Genomes Institute (http://genome.ornl.gov/microbial/csac). OligoArray 2.0 (Rouillard, 2003) was used to generate one 60-mer oligonucleotide probe sequence for each open reading frame. The probes were synthesized (Integrated DNA Technologies, IA), re-suspended in 50% DMSO, and printed onto Ultragap microarray slides (Corning, NY) using a QArrayMini arrayer (Genetix, UK). Each probe was spotted five times onto each array to fortify statistical analysis.


title: NIBS-Arabidopsis-solexa-AGO sRNAs

status: Public on Apr 05 2008

city: Beijing

last: Cai

address: No. 7 Science Park Road, Zhongguancun Life Science Park

country: China

institute: National Institute Of Biological Sciences, Beijing (NIBS)

description: Solexa, CSHL


title: JCVI PFGRC Streptococcus pneumoniae 21K v7 array designed primarily based on strain TIGR4

first: PFGRC


title: JCVI PFGRC Haemophilus influenzae 11K v2 array designed primarily based on strain biotype_aegyptius_F3031


title: JCVI PFGRC Campylobacter jejuni 23K v1 array designed primarily based on strain 81-176


title: JCVI PFGRC Haemophilus influenzae 11K v2 array designed primarily based on strain RD_KW20


title: JCVI PFGRC Streptococcus pneumoniae 21K v7 array designed primarily based on strain G54


title: SAGE:17:NlaIII:Botryotinia fuckeliana

status: Public on Apr 04 2008

last: GEO

organism: Botrytis cinerea

technology: SAGE NlaIII

description: This is a virtual platform for SAGE libraries generated for Botryotinia fuckeliana using NlaIII as an anchor enzyme.


title: JCVI PFGRC Haemophilus influenzae 11K v2 array designed primarily based on strain R2866


title: JCVI PFGRC Haemophilus influenzae 11K v2 array designed primarily based on strain pF3031


title: MaPTOL for REST

status: Public on Apr 01 2008

city: San Diego

first: Olivier

last: Harismendy

address: 10550 North Torrey PInes Road

department: Neurobiology

institute: The Scripps Research Institute

first: Jason

last: Iacovoni

first: Robyn

last: Meech

manufacture_protocol: Chromatin was prepared from mouse E12.5 embryos, sheared by sonication, and precleared with Protein-G agarose beads according to {Weinmann, 2002 #6}. An antibody (REST-H290 Ð Santa Cruz) targeted against the N-terminal region of mouse REST was used for immunoprecipitation under standard ChIP conditions {Weinmann, 2002 #6} with the following modifications. Two rounds of immunoprecipitation were carried on successively. During the second ChIP, after washing the bead-chromatin complex, they were equilibrated in NEB restriction enzyme buffer 4 with 0.5 % NP40 to sequester SDS. 80 µl of beads-chromatin slurry were then digested for 6hrs with 80 units of NlaIII. This step facilitates cloning of the co-immunoprecipitated DNA fragment and also provides footprint information as proteins that are bound to the DNA may prevent cleavage. After digestion, the complexes were again washed, eluted, and crosslinks reversed according to standard protocols {Weinmann, 2002 #6}. The entire eluate was cloned into the tagging vector pTagBM that contains two head to head MmeI restriction sites flanking the SphI cloning site. This initial library was amplified in bacteria (ECLONI Ð Lucigen) and purified using Invitrogen MaxiPrep Filter kit. 5 µg of this library was digested by 192 U of MmeI restriction enzyme overnight. After inactivation of the enzyme, we blunted the cut plasmid by incubation with 6U of T4 DNA polymerase and 10 pmol of dNTP during 3hrs at 14¼C. We stopped the reaction by addition of 10 mM EDTA for 20 min at 75¼C. The cut library was then purified by separation on an agarose gel and gel extraction using Qiagen Gel Extraction kit. 50 ng of the library was religated by overnight incubation at 14¼C with 1 µl of Invitrogen T4 DNA ligase. The religated library was used to transform E. coli competent cells (ECLONI Ð Lucigen). After quality control for religation by PCR, the library was amplified by growth on LB agar dishes (25x25 cm) and purified using Invitrogen MaxiPrep Filter kit. 100 µg of the library was digested overnight by 500 U of BamH1 restriction enzyme which sites encompass the two Mme1 sites on the pTagBM vector. The MaPTags extracted during this process were purified on an 8% polyacrylamide-TBE gel and electro-eluted from the gel (BioRad). 10 µg of MaPTags were subjected to sequencing.


title: JCVI PFGRC Haemophilus influenzae 11K v2 array designed primarily based on strain R2846

last: JCVI


title: IPMC - Coxiella burnetti 2k

city: Valbonne - Sophia-Antipolis

first: Kevin

last: Lebrigand

address: 660 route des lucioles

institute: IPMC/CNRS

organism: Coxiella burnetii

description:
Pangenomic array concerning Coxiella burnetii specie,
based on sequence accession number : NC_002971.

last: Le Brigand

first: Pascal

last: Barbry

manufacture_protocol: Spotted microarray


title: IPMC - microRNA v6

organism: Homo sapiens

first: Mascal

first: Bernard

last: Mari


title: Print_1186

city: Amsterdam

first: Cornelis

last: Verweij

department: Dept. of Pathology

institute: VU Medical Center


title: WUSTL Human Herpesvirus 1

city: St. Louis

first: Seth

middle: Daniel

last: Crosby

address: 660 S. Euclid

department: Genetics

institute: Washington Univeristy School of Medicine

organism: Human alphaherpesvirus 1

description: To construct a gene-specific microarray of the Human Herpesvirus 1 (Herpes simplex virus type 1) genome, we designed 70-mer oligonucleotide array elements.  Predicted and known HHV-1 genes were obtained from the strain 17 sequence on the NCBI  website (http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=NC_001806).  In addition, genes derived from mouse, common marker and selection genes, and mutant virus sequences were included.  Arabidopsis elements were included for normalization.  Oligonucleotides were designed from these sequences using ArrayOligoSelector.

manufacturer: Washington University School of Medicine

first: Diane

last: Alexander

first: Tracy-Jo

last: Pasieka

first: David

last: Leib

manufacture_protocol: Oligos were synthesized using standard methods by Illumina (San Diego, CA). These oligonucleotides, as well as Stratagene control A. coli sense oligos #7 and #8 (Ambion part# 1781), were dissolved at a concentration of 50 μM in 3X SSC with 0.75M betaine and were printed in duplicate on MWG Epoxy slides by a locally constructed linear servo arrayer (after the DeRisi model, http://derisilabs.ucsf.edu/).


title: Print_1366

first: Daniel

last: Hogan


title: JCVI PFGRC Campylobacter jejuni 23K v1 array designed primarily based on strain RM1221

city: Rockville


title: JCVI PFGRC Campylobacter jejuni 23K v1 array designed primarily based on strain RM1170


title: Print_1355


title: BioStarH-141s

status: Public on Sep 05 2007

city: Shanghai

first: xuping

last: fu

institute: Institute of Genetics, Fudan University

manufacture_protocol: The construction of the microarrays used in this study was carried out following Brown’s method (available at http://cmgm.stanford.edu/pbrown/protocols/index.html).We fabricated a “genome-wide” cDNA microarray consisting of 14061 sequences including full-length and partial cDNAs representing novel, known, and control genes provided by United Gene Holdings Group Co., Ltd. Genes represented in the arrays were identified with high similarity to the sequences in Unigene database of NCBI by performing Blast (http://www.ncbi.nlm.nih.gov/).The cDNA inserts were amplified by polymerase chain reaction (PCR) with universal primers to plasmid vector sequences and then purified. All the PCR products were examined by agarose gel electrophoresis to ensure the quality and identity of the amplified clones as expected. Then the amplified PCR products were dissolved in a buffer containing 3ХSSC solution. The solution with amplified PCR products were spotted onto silylated slides(CEL Associates, Houston, TX) using a Cartesian PixSys 7500 motion control robot (Cartesian Technologies, Irvine, CA) fitted with ChipMaker Micro-Spotting Technology (TeleChem International, Sunnyvale, CA). Glass slides with spotted cDNA were then hydrated for 2 hr in 70% humidity, dried for 0.5 hr at room temperature, and UV cross-linked (65mj/cm). They were further processed at room temperature by soaking in 0.2% sodium dodecyl sulfate (SDS) for 10 min, distilled H2O for 10 min, and 0.2% sodium borohydride (NaBH4) for 10 min. The slides were dried again and ready for use. The fluorescent cDNA probes were prepared through reverse transcription of the isolated mRNAs and then purified according to the methods of Schena et al.. The cancerous samples and normal samples were labeled with Cy5-dUTP. The common reference samples ,a pool of RNA from mixed human embryo tissues,were labeled with Cy3-dUTP. The two color probes were then mixed, precipitated with ethanol, and dissolved in 20μl of hybridization solution [5ХSSC (0.75 M NaCl and 0.075 M sodium citrate), 0.4% SDS, 50% formamide, and 5ХDenhardt’s Solution (0.1% Ficoll, 0.1% polyvinylpyrrolidone, and 0.1% BSA)]. Resultant cDNA was purified and hybridized to the microarray provided by United Gene Holdings Group Co., Ltd. Microarrays were pre-hybridized with hybridization solution containing 0.5 mg/ml denatured salmon sperm DNA at 42℃ for 6 hr. Fluorescent probe mixtures were denatured at 95℃ for 5 min, and the denatured probe mixtures were applied onto the pre-hybridized chip. And then put a cover glass on it. Chips were hybridized at 42℃ for 15–17 hr. The hybridized chips were then washed at 60℃ for 10 min each in solutions of 2ХSSC and 0.2% SDS, 0.1ХSSC and 0.2% SDS, and 0.1ХSSC, and then dried at room temperature.Every slide was scanned by ScanArray 4000B scanner (General Scanning, Watertown, MA). The fluorescent images of Cy5 and Cy3 were scanned separately and saved for further image analysis. The acquired images were analyzed with GenePix Pro 3.0 software (Axon Instruments, Inc., Union City, CA).


title: JCVI PFGRC Campylobacter jejuni 23K v1 array designed primarily based on strain 269.97


title: SHGS

status: Public on Jul 19 2007

last: Stanford Microarray Database

address: 300 Pasteur Drive

department: Stanford University, School of Medicine

institute: Stanford Microarray Database (SMD)

manufacturer: Stanford Functional Genomics Facility,  Stanford School of Medicine


title: MPI_Chen_domestic_dog_Solexa_2007

first: wei

last: chen

organism: Canis lupus familiaris


title: OSUrbt Oncorhynchus mykiss 1.6K v2.0 update 07.05.07

status: Public on Jul 13 2007

city: Logan

first: Abby

last: Benninghoff

state: UT

address: 4815 Old Main Hill

department: Animal, Dairy and Veterinary Sciences

institute: Utah State University

organism: Oncorhynchus mykiss

description: 4 X 4 pin arrangement Microspot 2500 pins (quill design)

first: Susan

last: Tilton

first: Christopher

last: Bayne

first: Scott

last: Givan

first: Caprice

last: Rosato

last: Williams

manufacture_protocol: Oligonucleotides were resuspended in spotting buffer (3X SSC and 1.5 M betaine) at 25 uM final concentration and were printed onto Corning UltraGap slides (Acton, MA) using a Biorobotics Microgrid II arrayer (Genomic Solutions, Ann Arbor, MI) with 16 pins. The trout 70-mers were each printed in duplicate and a set of ten SpotReport Alien Oligos (Stratagene, La Jolla, CA) were printed 16 times (once per block) on the array. After printing, slides were desiccated for 24-48 hrs and cross-linked with UV Stratalinker (Stratagene) at 600 mJ.


title: UDMP Homo sapiens MitoArray 6k v1

status: Public on Jul 01 2007

city: Prague

first: Viktor

last: Stranecky

address: Ke Karlovu 2

department: 1st Faculty of Medicine

country: Czech Republic

institute: Charles University

description:
Microarray: 5760 spots
24 blocks: 4x6
each block: 15x16 spots

manufacturer: Institute of inherited metbolic disorders, Charles University, First Faculty of Medicine in Prague

first: Alena

last: Cizkova

first: Robert

last: Ivanek

first: Hana

last: Hartmannova

first: Lenka

last: Noskova

last: Piherova

first: Marketa

last: Tesarova

last: Hansikova

first: Tomas

last: Honzik

first: Jiri

last: Zeman

first: Petr

last: Divina

first: Jan

last: Paul

first: Josef

last: Houstek

first: Stanislav

last: Kmoch

manufacture_protocol: For each of selected 1632 genes, a single 5´-aminomodified 40-mer oligonucleotide was designed using Oligopicker software (see the Parallel Biological Systems web page, by the Massachusetts General Hospital, Harvard Medical School, and Boston University).  Blast searches were performed with each candidate probe to exclude possibility of cross hybridization with homologous genes prior the synthesis oligonucleotide probes. Synthesized oligonucleotides, Generi Biotech (Czech Republic) and Illumina (San Diego, Ca), were resuspended at 20µM concentration in 3*SSC, printed in triplicates on aminosilane modified slides and immobilised by standard technique using combination of backing and UV cross-link. Qualities of arrays from individual printing series were assessed using fluorescently labelled panomers (Invitrogen, Carlsbad, Ca).


title: Filgen Array Human25K

status: Public on Mar 24 2007

city: Akita

first: Atsushi

last: Komatsuda

address: Hondo 1-1-1

institute: Akita University School of Medicine

first: Manabu

last: Harada


title: Vulpe Lab UC-Berkeley Fathead Minnow v1.0

city: Miami

first: Edward

last: Mager

state: FL

address: 4600 Rickenbacker Cswy.

department: MBF

institute: University of Miami, RSMAS

organism: Pimephales promelas

first: Chris

last: Vulpe

first: Henri

last: Wintz

manufacture_protocol:
1.	A fathead minnow cDNA library was constructed in lambda TriplEx2 using the SMART cDNA kit (Clonetech, Mountain View, CA) using a pool of RNA extracted from unexposed fathead minnow eggs, and fish of different ages (less than 5, 5-10, 10-60, 60-150 and more than 150 day-old).
2.	The library was amplified, plated and 5,000 randomly selected plaques were grown in 96 well plates to produce phage supernatant.
3.	1 µl of phage supernatant was used as template to PCR amplify the cDNA inserts using two custom made oligonucleotides encompassing the SfIA cloning sites (SfiA: GGCCATTACGGCCGGG and SfiB: CGAGAGGCGGCCGACAT). PCR reactions were carried out in 96 well plates in a 50 µl volume using an amplification program consisting of 3 min at 94°C, followed by 30 cycles of 30 sec at 94°C, 1 min at 65°C, 2 min at 72°C.
4.	A sample of a subset of the reactions from each 96 well plate were analyzed on agarose gel.
5.	All PCR reactions were cleaned by ethanol precipitation, followed by a washing with 70% ethanol and dissolved overnight in 50 µl of  3xSSC under gentle shaking.
6.	PCR reactions were arrayed in Corning polypropylene 384 well plates using a Packard Bioscience Multiprobe IIex liquid handler (Packard .
7.	Printing was done on polyamine coated slides using a custom made printer equipped with capillary print tips. Each cDNA was spotted twice.


title: BC Cancer Agency_E.coli_H.influenzae_P.aeruginosa_HumanChr6_340K array v1

city: Vancouver

first: Rene

middle: Louis

last: Warren

state: British Columbia

address: 570 West 7th avenue Suite 100

department: Genome Sciences Centre

institute: BC Cancer Agency

organism:
Pseudomonas aeruginosa
Escherichia coli
Haemophilus influenzae
Homo sapiens

first: Duane

last: Smailus

first: Stephane

last: Flibotte

last: Holt

first: Doug

last: Freeman

manufacture_protocol:
Oligonucleotide array design
An oligonucleotide microarray has been designed to simultaneously measure the RNA level from genes in three different bacterial species (H. influenzae, E. coli and P. aeruginosa) and probe the DNA from one human BACs.  DNA oligonucleotides of length 60 targeting most genes were selected with the help of various filters designed to maximize the sensitivity and specificity of each oligonucleotide and the resulting signal-to-noise ratio. The filters and associated computer codes were similar to the ones successfully used for arrays designed to study C. elegans (Maydan et al. 2007).  Briefly, oligonucleotides containing 20-mers appearing more than once in the genomes of interest or having an overall identity above 75% with non-target sequences were eliminated from the pool of potential probes. Oligonucleotides presenting a significant self-folding energy or homopolymer longer than 5 bases in length were also eliminated and only the oligonucleotides with GC content between 36% and 62% were kept for further consideration, which corresponds to a range of about 10 ºC in melting temperature Tm. In the case of the human BACs, the selection process also eliminated known DNA repeats. The oligonucleotides appearing in the final design were selected from the pool of potential probes obtained with the above-mentioned filters taking into consideration the 14-mer count, 15-mer count, GC content, overlap between neighboring probes, and the number of probes per gene. The k-mer count of an oligonucleotide is defined as the sum of the frequencies within the genomes of interest of all constituent k-mers within the oligonucleotide. NimbleGen Systems manufactured the microarrays with each selected 60-mer oligonucleotide synthesized at random positions.


title: JCVI PFGRC Haemophilus influenzae 11K v2 array designed primarily based on strain 86-028NP

status: Public on Apr 09 2008

first: PFGRC

last: JCVI

department: PFGRC

manufacture_protocol: 2QHI, with arrayer LUCIDEA, mode Normal, replicates 2, repeats 1, slide_dips 40, machine cycles 1099390, pen id 186, pen cycles -1, operator Robin/Chris, humidity control On, humidity set point65


title: LLNL Virulence Mechanism Array, version 2A

status: Public on Apr 02 2008

city: Livermore

first: Crystal

last: Jaing

address: 7000 East Ave.

institute: Lawrence Livermore National Laboratory

organism:
Staphylococcus aureus subsp. aureus Mu50
Escherichia coli CFT073
Enterococcus faecalis V583
Escherichia coli str. K-12 substr. MG1655

first: Shea

middle: N

last: Gardner

first: Kevin

middle: S

last: McLoughlin

first: Peter

last: Williams

first: Tom

last: Slezak

first: Nisha

last: Mulakken

manufacture_protocol: see the Nimblegen website for details


title: IOLR_Cherax quadricarinatus_4.8K_v1.0

status: Public on Jan 30 2007

city: Haifa

first: Moshe

last: Tom

department: Marine Biology and Biotechnology

country: Israel

organism: Cherax quadricarinatus

description: The array is printed on a 2.5 x 7.5 cm glass (GAPS II slides, Corning) on an area less than 6 x 2 cm. the grid system includes 4 x 12 sub-arrays, each containing 15 raws and 14 columns, a total of 10080 spots.

manufacturer: Moshe Tom's laboratory - Israel Oceanographic and Limnological Research, Haifa, Israel.

first: Yana

last: Yudkovski

first: Vered

last: Chalifa-Caspi

first: Meirav

last: Auslander

first: Asaf

last: Shechter

first: Amir

last: Sagi

manufacture_protocol:
X Organ – Sinus Gland complex was bi-laterally extirpated from several C. quadricarinatus males, followed after 13 or 14 days post-extirpation by sacrifice of premolted males, monitored by their gastrolith size, maxillar setae appearance and in some individuals the presence of new cuticle under the old one. Several hepatopancreatic lobes and hypodermis layered beneath the carapace and muscle tissue were dissected as well as the entire Y organ pair and RNA was extracted from the dissected tissues. The muscle served as a reference organ, whereas the molt-related hepatopancreas, epidermis and Y-organs were the target organs. The cDNA assemblage was constructed in two stages: (1) SMART (Switching Mechanism At 5' end of the RNA Transcript), aimed at amplification of reverse transcribed cDNAs using a universal primer, linked to both ends of the template cDNA during the reverse transcription stage (Clontech, manufacturer instructions). (2) SSH (Subtractive-Suppression Hybridization method; Diatchenko et al. 1999; PCR-select, Clontech), aimed at T-A cloning of a roughly equally represented cDNAs from the target organs into an expression plasmid (pGEM-Teasy, Promega). biased towards genes differentially expressed in the target tissues in comparison to the reference one. The cloned cDNAs were transfected into E. coli, and 4800 bacterial colonies were isolated, amplified and frozen at -70C. A subset of the clones were successfully sequenced and assembled by the CAP3 software (Huang and Madan, 1999), to identify unique sequences. All the 4800 clones were amplified by standard PCR from 1 µl bacterial culture templates (5’ at 95C; 32 cycles of 0.5’ at 95C, 0.5’ at 68C and 1.5’ at 72C; a final incubation of 4’ at 72C; GeneAmp 5700, ABI). PCR products were precipitated from the PCR solution by one volume of isopropanol at room temperature. The dry DNA was dissolved in printing buffer at final concentration of 50% DMSO and 0.3 x SSC and was printed on a glass slide (GAPS II slides, Corning; MGII robot-printer, BioRobotics) in duplicates. The amplification efficiency was tested by running the PCR products on a 1% agarose gel concurrent with a DNA mass ladder. The printing pattern included two complete and separate sets of the transcriptome, in the upper and lower part of the slide, respectively.
Refernces:
Diatchenko, L., Lukyanov, S., Lau, Y.F.C. Siebert, P.D. (1999) uppression subtractive hybridization: A versatile method for identifying differentially expressed genes. In: cDNA Preparation and characterization, Meth. Enzymol. 303: 349-380.
Huang, X. and Madan, A. (1999) CAP3: A DNA sequence assembly program. Gen Res 9: 868-877.


title: AgResearch Bos taurus Clone Array Print 114

status: Public on Feb 20 2008

city: Puddle Alley

first: Alan

middle: Francis

last: McCulloch

state: Mosgiel

address: Invermay

country: New Zealand

institute: AgResearch NZ

organism: Bos taurus

middle: F

first: Dianne

last: Hyndman

manufacture_protocol:
Microarray preparation
The Bovine ESTs used for the cDNA microarry  were made and sequenced by the Genesis Company Auckland NZ . The 23K array consisted  of  EST  from 50 tissue libraries covering  brain, mammary, heart and skeletal muscle, glands, ovaries, fat, liver, intestine, spleen and PBM cells..
Clones in pBK-CMV vector were amplified by 55pmol primers T7 anad T3 and those in pcMV-sport6 by 45pmol T7 and Sp6  in a 50ul reaction with 0.2mM dNTPs, 1.5mM mgCl and 2.5 units AB red hot taq polymerase in 96 well plates.  The  PCR was carried out at 55ºC for 36 cycles and extended at 72ºC for 5min.
The product was verified by running a portion on a 1% gel. The sample were then purified by isopropanol and ETOH precipitation.

The cDNA samples were suspended in 5ul 3x SSC giving a concentration of 100 to 200ng.
Printing was performed on poly L Lysine coated slides using split pin technology on the ESI array robot with a 32 pin configuration.


title: Yale University MCDB Medicago truncatula NimbleGen 390K Oligo Array

status: Public on Jan 15 2008

city: New Haven

first: Hang

last: He

state: CT

address: 165 Prospect St

department: MCDB

institute: Yale University

organism: Medicago truncatula

first: Lei

last: Li

first: Juan

last: Zhang

first: Xiangfeng

last: Wang

first: Viktor

last: Stolc

first: Waraporn

last: Tongpraist

first: Oliver

last: Yu

first: XingWang

last: Deng

manufacture_protocol:
1. Oligo length: 36
2. Minimum oligonucleotide Tm: 80
3. Maximum oligonucleotide Tm: 95
4. Temperature to use during secondary structure prediction (an oligo will be rejected if it can fold into a stable secondary structure at this temperature): 65
5. Threshold to report putative cross-hybridizations (all targets hybridizing with a given oligo with a Tm exceeding this threshold are reported): 70
6. Minimum oligonucleotide GC content: 40
7. Maximum oligonucleotide GC content: 73 for gene models; 77 for TARs
8. Minimum distance between the 5' end of two adjacent oligos: 1
BAC fasta and description files linked as supplementary files


title: Yale University MCDB Glycine max NimbleGen 390K Oligo Array

organism: Glycine max


title: Yang et. al. Oligoarray of CfMNPV select ORFs

status: Public on May 01 2007

city: Guelph

first: Dan-Hui

last: Yang

address: 488 Gordon Street

department: Dept. of Molecular and Cellular Biology

institute: University of Guelph

organism: Choristoneura fumiferana multiple nucleopolyhedrovirus

description: The array included 46 oligonucleotides (1.0 µg/µl) representing 23 CfMNPV ORFs, one for the coding strand and the other for the non-coding strand.  Purified viral genomic DNA (50, 25, 5 ng/µl) was used as a positive control. A mixture of 18 viral oligonucleotides (50 ng/µl each) was served as a second positive control. The array included 2 oligos (At1 and At2) of Arabidopsis chlorophyll synthase gene to be hybridized with the spike RNA, and 2 oligos (HAT4, YaaJ) as negative controls. The array also included 5 insect internal control oligos. Each probe was printed in triplicate.

manufacturer: Biotechnology Research Institute, Montreal, Canada

first: Mehrnoosh

last: Barari

first: Peter

last: Krell

manufacture_protocol: Spotted 70 mer nucleotides on glass


title: Hebrew University Mus musculus SpliceChip

status: Public on Dec 05 2007

city: Jerusalem

first: Ari

last: Meerson

address: Givat Ram

institute: Hebrew University

organism: Mus musculus

first: Shani

last: Ben-Ari

first: Hermona

last: Soreq

manufacture_protocol: To compose the SpliceChip, C6 amino-modified 70-mer oligonucleotides from the Operon mouse genome oligo set version 3.0 (Operon, Huntsville, AL, USA) were dissolved in spotting buffer [3 × saline sodium citrate (SSC), 1.5 m betain] to a final concentration of 20 mm, and printed on sialyated (SAL-1) Genorama slides (AsperBiotech, Tartu, Estonia) using the MicroGrid spotter (Genomic Solutions, Holliston, MA, USA). The array layout (17.76 × 13.26 mm) contains 12 subgrids, each composed of 12 rows and 12 columns. Tested oligonucleotides were spotted six times each on the array; negative controls were spotted 18 times.


title: Hebrew University Mus musculus SpliceChip (condensed template)

status: Public on Dec 07 2007


title: INSERM MWG human 23k oligo array_version 3

status: Public on Mar 04 2008

city: NANTES CEDEX 1

first: Audrey

last: Bihouee

address: 8 quai Moncousu

institute: Université de Nantes

first: Catherine

last: Chevalier

first: Isabelle

last: Guisle

manufacture_protocol: MWG-designed oligo probes spotted in triplicate using the Amersham Lucidea spotter. Epoxyslide. Hygrometry=65%


title: CEA - DSV/DRR/SGF : AFJ

status: Public on Oct 03 2006

city: Evry

first: Cyrille

last: Petat

address: 2, rue Gaston Crémieux

department: DSV/DRR

institute: CEA

organism:
Mus musculus
Rattus norvegicus

first: Amelie

last: Robert

first: Peggy

last: Maltere

first: Franck

last: Amiot

manufacture_protocol: Chip contains 9216 features (probes and controls):  2014 PCR products from cDNA matrix using primers specific of genes involved in important biological process (apoptosis, cell cycle, stress response).  1684 PCR products from cDNA clones from the I.M.A.G.E consortium (Research Genetics collection).  2014 PCR products from mouse cDNA clones of a substractive bank (myodistrophic versus normal muscle).  1800 PCR products from rat cDNA clones of a substractive bank (atrophied versus normal muscle).  1704 control reporters.


title: Agilent Human 1 cDNA Microarray (G4100A) [layout C]

status: Public on Aug 25 2007

city: Milan

first: Pietro

last: Mariani

address: Via La Masa 19

institute: Mario Negri Institute

description: layout: portrait, barcode location: bottom, scan side: front. Gal file annotations.

manufacture_protocol: see the manufacturer website http://www.agilent.com


title: Mouse oligo array from MWG version 2

status: Public on Aug 08 2006

city: Saint-Genès-Champanelle

last: CASSAR-MALEK

address: Theix

department: Centre de Clermont-Fd/Theix

institute: INRA

manufacture_protocol: spotted on the  Amersham Lucidea spotter. Epoxyslide. Hygrometry=65%


title: CEA - DSV/DRR/SGF : APZ Mouse and Rat 23k

status: Public on Jun 22 2006

manufacture_protocol: MicroArray contains 23040 features (probes and controls):14592 PCR products from cDNA clones from early mouse embryo libraries constructed by the National Institute of Aging (NIA); 2016 PCR products from cDNA matrix using primers specific of genes involved in important biological process (apoptosis, cell cycle, stress response); 1632 PCR products from cDNA clones from the I.M.A.G.E consortium (Research Genetics collection); 1920 PCR products from mouse cDNA clones of a substractive bank (myodistrophic versus normal muscle); 1728 PCR products from rat cDNA clones of a substractive bank (atrophied versus normal muscle); 1152 control reporters.


title: ORNL Skin cDNA array

status: Public on Apr 18 2006

city: Augusta

first: Yutao

last: Liu

state: GA

address: 1120 15th Street

department: Cellular Biology and Anatomy

institute: Augusta University

description: cDNA clones are from Research Genetics

first: Brynn

last: Voy

manufacture_protocol:
Detailed protocol referred to
Hegde P, Qi R, Abernathy K, Gay C, Dharap S, Gaspard R, Hughes JE, Snesrud E, Lee N, Quackenbush J.  A concise guide to cDNA microarray analysis. Biotechniques. 2000 Sep;29(3):548-50, 552-4, 556 passim.


title: GE Healthcare/Amersham Biosciences CodeLink UniSet Human I Bioarray

status: Public on Sep 05 2006

city: Giessen

first: Hamid

last: Hossain

address: Frankfurter Str. 107

department: University of Giessen

institute: Institute for Medical Microbiology

first: Felix

last: Thierer

manufacture_protocol: see GE Healthcare/Amersham Biosciences website


title: CEA_SGF:AJX

status: Public on Feb 10 2006

first: Amélie

manufacture_protocol: The mouse chip consists of a large set of 13 056 cDNA clones from early mouse embryo libraries constructed by the National Institute of Aging (NIA) (http://www.nia.nih.gov) representing about 11 000 genes. It also includes positive (actin, tubulin, oligodT, cot-1 DNA) and negative (TE/DMSO) controls present many times in the array for quality control.


title: platform_2004-11-09_Human_60mer_1in2

status: Public on Aug 31 2007

city: Notre Dame

first: Randy

last: Jeffrey

state: IN

address: Rm 232, Galvin Life Sciences Bldg

department: Biological Sciences

institute: University of Notre Dame

first: Somdutta

last: Roy

first: Martin

last: Tenniswood

manufacture_protocol: 1.  Please Refer to Nimblegen Systems


title: SHCX

status: Public on Dec 06 2005

city: Stanford

first: Jonathan

last: Pollack

state: CA

address: 269 Campus Drive, CCSR3245A

department: Pathology

institute: Stanford University

description:
coating = unknown_surface_type
Support = glass
Platform_manufacturer: Joan,,Hebert; SFGF, 2255, CCSR, Stanford, Ca, USA, 94025; 650-736-0075; jhebert@genome.Stanford.edu
Platform_technology: other


title: SHEG

description:
Coating = unknown_surface_type
Support = glass
Platform_distribution: non-commercial
Platform_manufacturer: Joan,,Hebert; SFGF, 2255, CCSR, Stanford, Ca, USA, 94025; 650-736-0075; jhebert@genome.Stanford.edu
Platform_technology: other


title: Nimblegen channel catfish 382K oligo array version 1.0

status: Public on Dec 01 2005

city: Stoneville

first: Geoffrey

middle: C.

last: Waldbieser

state: MS

address: 141 Experiment Station Road

department: Agricultural Research Service

institute: U.S. Dept of Agriculture

organism: Ictalurus punctatus

description: This set includes 24-mer oligonucleotides for 18,999 catfish expressed sequences.  The design was based upon available catfish sequences (June 2004) clustered using CAP3.

first: Robert

last: Li

manufacture_protocol: NimbleGen proprietary software used to identify 10 perfectly matched and 10 mismatched 24-mer oligonucleotides for each catfish gene.  Some sequences were not long enough to provide for design of 10 oligos.


title: Fundulus heteroclitus array #1

status: Public on Jul 15 2005

city: Ocean Springs

last: Roling

address: 703 East Beach Dr

department: Department of Coastal Sciences

institute: University of Southern Mississippi

organism:
Fundulus heteroclitus
Pseudopleuronectes americanus

first: William

last: Baldwin

middle: A

first: Nikki

middle: L

last: Maples

first: Janis

last: Peterson

first: Lisa

last: Bain

manufacture_protocol: see www.EcoArray.com


title: Atlas Plastic Human 12K Microarray (Cat. #634811)

status: Public on Dec 25 2006

city: Albquerque

first: Susan

middle: Esther

last: Boggs

state: NM

address: 2425 Ridgecrest Dr SE

institute: Lovelace Respiratory Research Institute

description: Plastic microarrays made with long (~80 bp) experimentally-tested oligonucleotides.

middle: E

first: Jay

last: Aden

first: Brandy

last: Crowder

manufacture_protocol: see manufacturer's web site


title: CEA - DSV/DRR/SGF : Human 25k oligo v1.0

status: Public on Feb 02 2007

first: Cyrille

last: Petat

first: Amelie

last: Robert

first: Peggy

last: Maltere

first: Franck

last: Amiot

manufacture_protocol: Chip contains 26496 features (probes and controls): 26068 oligonucleotides representing 21000 genes (Le Brigand K et all, An open-access long oligonucleotide microarray resource for analysis of the human and mouse transcriptomes. Nucleic Acids Res. 2006 Jul 19;34(12)); 428 control reporters. Oligos printed in phosphate buffer with MicroGridII (BioRobotics).


title: Mouse Compugen 22K oligo

status: Public on Jul 20 2005

city: St Lucia

first: Darrin

last: Taylor

state: Qld

address: Research Rd

department: Genomics and Bioinformatics

country: Australia

institute: Institute for Molecular Bioscience, Uni of Qld

description: Mouse Compugen 22K oligo


title: UMCU Human 19k array v1

status: Public on Mar 02 2007

city: Zeist

first: Sonia

last: Pavan

address: Utrechtseweg 48

department: Physiological Sciences

institute: TNO Quality of Life

manufacturer: UMC Utrecht-Microarray Facility-Genomics Lab

manufacture_protocol: Human slides containing 19200 spots (16659 70-mers, Operon human version1, and 2541 control spots). The microarrays are printed onto Corning UltraGAPS slides. Scanning of the slides is performed at 10 micron resolution


title: CEA - DSV/DRR/SGF : AHF


title: SHFB

status: Public on Jun 08 2005

last: Stanford Microarray Database

address: 300 Pasteur Drive

department: Stanford University, School of Medicine

institute: Stanford Microarray Database (SMD)

description:
Support: glass
Coating: unknown_surface_type
Manufacturer: Stanford Functional Genomics Facility,  Stanford School of Medicine


title: Ion Channel Splice Array

status: Public on Jan 23 2007

city: New York

first: Erin

middle: L.

last: Heinzen

state: NY

department: Institute for Genomic Medicine

institute: Columbia University

first: David

middle: B

last: Goldstein

manufacture_protocol: Splice microarray, uses 3-6 probes (both exon-exon junction and purely exonic probes) to screen for the presence of alternatively splice events


title: lung_development_Nmyc_mouse

status: Public on Apr 24 2007

city: Toronto

first: Brian

middle: Joseph

last: Cox

state: Ontario

address: 1 King's College Circle, Rm 3360

department: Physiology

institute: University of Toronto

technology: MS

description:
Virtual platform describing the full complement of proteins identified in the study of lung development from E13.5 to adult. Also included are proteins identifed in E18.5 Nmyc9a hypomorphic lungs.

The data set was generated by MudPIT mass spectrometry analysis of embryonic lungs at E13.5, E16.5 and E18.5 and postnatal days P2, P14 and Adult (>8 weeks). The lungs were fractionated into nuclear, cytosol and mitochondria enriched fractions by sucrose gradient centrifugation. The extracted proteins were digested with trypsin and analyzed by MudPIT.

first: Thomas

last: Kislinger

first: Andrew

last: Emili

first: Janet

last: Rossant


title: SHFC


title: Genopole Mouse oligo array from MWG version 1

status: Public on Aug 19 2006

first: Isabelle

last: CASSAR-MALEK

organism:
Homo sapiens
Mus musculus

first: Catherine

last: Chevalier

last: Guisle


title: SHEM

status: Public on May 27 2005

first: Markus

last: Bredel

institute: Stanford University School of Medicine

organism: Homo sapiens

description:
Support: glass
Coating: unknown_surface_type
Platform_manufacturer: Joan,,Hebert; SFGF, 2255, CCSR, Stanford, Ca, USA, 94025; 650-736-0075; jhebert@genome.Stanford.edu


title: SHBR

status: Public on May 25 2005

city: San Francisco

first: Xin

last: Chen

institute: UCSF

description:
SHBR
Platform_coating: unknown_surface_type
Platform_distribution: non-commercial
Platform_manufacturer: Joan,,Hebert; SFGF, 2255, CCSR, Stanford, Ca, USA, 94025; 650-736-0075; jhebert@genome.Stanford.edu
platform_support: glass
Platform_technology: other


title: CEA - DSV/DRR/SGF : APV Mouse and Rat 23k

status: Public on Jun 22 2006

last: Petat

institute: CEA

last: Robert


title: CE_printU

status: Public on Sep 13 2006

city: Palo Alto

first: Michael

last: Shapira

address: 300 Pasteur Dr. Lane Bldg. room L309

department: Genetics

organism: Caenorhabditis elegans

manufacturer: John,,Matese; Lewis-Sigler Institute for Integrative Genomics, Carl Icahn Laboratory, Room 135, Princeton, NJ, United States; (609) 258-8309; jcmatese@genomics.princeton.edu

manufacture_protocol: Material is transferred from microtiter plate wells to the slide surface by an automated print head


title: CEA - DSV/DRR/SGF : ANG

status: Public on Sep 07 2006

first: Cyrille

last: Maltere

last: Amiot

manufacture_protocol: Chip contains 8832 features (probes and controls):  2304 PCR products from cDNA matrix using primers specific of genes involved in important biological process (apoptosis, cell cycle, stress response).  5760 PCR products from cDNA clones from human infant brain library (Soares et al. 1994).  768 control reporters and pseudo-features.


title: SHDN

status: Public on May 19 2006

middle: Yuan

last: Zhang

address: 770 Welch Rd, Fourth floor

department: Plastic and Reconstructive Surgery

institute: Stanford Hospital and Clinics

description:
Manufacturer: Joan,,Hebert; SFGF, 2255, CCSR, Stanford, Ca, USA, 94025; 650-736-0075; jhebert@genome.Stanford.edu
Platform_coating: unknown_surface_type
Platform_support: glass
Platform_technology: other


title: SHFM


title: CEA_SGF:AKH

status: Public on Feb 10 2006

first: Amélie

manufacture_protocol: The mouse chip consists of a large set of 12288 cDNA clones from early mouse embryo libraries constructed by the National Institute of Aging (NIA) (http://www.nia.nih.gov) representing about 11 000 genes. It also includes positive (actin, tubulin, oligodT, cot-1 DNA) and negative (TE/DMSO) controls present many times in the array for quality control.


title: CCMB Drosophila Array II

status: Public on May 15 2005

city: Hyderabad

first: L S

last: Shashidhara

state: AP

address: Uppal Road

country: India

institute: Centre for Cellular and Molecular Biology

organism: Drosophila melanogaster

description:
Primers developed by Incyte Genomics were used to PCR amplify one exon or exon fragment from every predicted gene in the fly genome, using genomic DNA from Cantos-S flies as template. The size, quality and quantity of PCR products were tested by electrophoresis; before and after purification. The array was assembled using amplicons representing approximately 4018 genes predicted in version 1.0 of the Drosophila genome from sequences released by the Berkeley Drosophila Genome Project and Celera Genomics in March 2000. In addition to 4018 genes, Drosophila DNA samples also included several negative controls, particularly intergenic regions.

Purified PCR products were concentrated on a speed-vac and resuspended in the printing buffer (0.04 M tris pH 6.5 ,0.1M KCl, 50% DMSO). Average concentration of the DNA used for sporting was ~400 picomoles in 20 ml volume. DNA was spotted in duplicates using the Gen III spotter from Molecular Dynamics on the Type V glass slides provided by Amersham Biosciences, USA. Samples were spotted at a density of 9216 spots per array. All the samples were spotted in duplicates, thus each array carried only 4608 independent samples. In addition to Drosophila DNA samples, each array consisted of as many as 384 spots for positive control, negative control, dynamic range control and ratio control (Amersham Lucidea ScoreCard). These were obtained from Amersham Biosciences. Except for these control spots, contents of CCMB Drosophila Array I and II are the same as in GPL20, although they they do not include all the elements of GPL20.

Spotting efficiency was evaluated by Cyber green (Sigma, USA) staining. Dyed or hybridized slides were scanned using Molecular Dynamics GenIII Scanner.

first: Mohit

last: Prasad

first: M

last: Madhavi

first: Ashish

last: Lal

first: Gururaj

last: Sirdesai

first: Ram

last: Reddy

first: V

last: Bharathi

first: Jyotsna

last: Dhawan

first: L


title: NADC MAP Oligo v1.0

status: Public on Feb 08 2006

city: Ames

first: Mike

last: Paustian

state: IA

address: 2300 Dayton Ave

country: USA

institute: National Animal Disease Center, USDA-ARS

organism:
Mycobacterium avium subsp. paratuberculosis
Mycobacterium avium subsp. avium

first: John

middle: P

last: Bannantine

first: Vivek

last: Kapur

manufacture_protocol: 70mer oligos were designed using ArrayOligoSelector. Input sequences included all annotated ORFs and intergenic regions > 500 bp from MAP K10 as well as all MAA 104 ORFs with < 30% identity to MAP K10. MAA 104 sequence was obtained from TIGR on 07/09/04 and automatically annotated using Reganor software (McHardy et al). One oligo was designed for each ORF < 2000 bp and two oligos were designed for each ORF > 2000 bp. Six Arabidopsis clones and MAP K10 sheared genomic DNA are included as controls. Oligos were synthesized by Illumina. The microarray was printed using a BioRobotics arrayer equiped with 48 quill tip pins. Each source well was printed in triplicate on the array.


title: CCMB Drosophila Array I

description:
Primers developed by Incyte Genomics were used to PCR amplify one exon or exon fragment from every predicted gene in the fly genome, using genomic DNA from Cantos-S flies as template. The size, quality and quantity of PCR products were tested by electrophoresis; before and after purification. The array was assembled using amplicons representing approximately 4084 genes predicted in version 1.0 of the Drosophila genome from sequences released by the Berkeley Drosophila Genome Project and Celera Genomics in March 2000. In addition to 4084 genes, Drosophila DNA samples also included several negative controls, particularly intergenic regions.

Purified PCR products were concentrated on a speed-vac and resuspended in the printing buffer (0.04 M tris pH 6.5 ,0.1M KCl, 50% DMSO). Average concentration of the DNA used for sporting was ~400 picomoles in 20 ml volume. DNA was spotted in duplicates using the Gen III spotter from Molecular Dynamics on the Type V glass slides provided by Amersham Biosciences, USA. Samples were spotted at a density of 9216 spots per array. All the samples were spotted in duplicates, thus each array carried only 4608 independent samples. In addition to Drosophila DNA samples, each array consisted of as many as 384 spots for positive control, negative control, dynamic range control and ratio control (Amersham Lucidea ScoreCard). These were obtained from Amersham Biosciences. Except for these control spots, contents of CCMB Drosophila Array I and II are the same as in GPL20, although they they do not include all the elements of GPL20.

Spotting efficiency was evaluated by Cyber green (Sigma, USA) staining. Dyed or hybridized slides were scanned using Molecular Dynamics GenIII Scanner.


title: SHEU

status: Public on Jan 24 2006

first: Edwin

last: Rodriguez

address: Beckman, B439

institute: UC Stanford

manufacturer: Joan,,Hebert; SFGF, 2255, CCSR, Stanford, Ca, USA, 94025; 650-736-0075; jhebert@genome.Stanford.edu


title: FHCRC Mouse RG 5k Array v1.0

status: Public on Feb 03 2006

city: Seattle

first: Denise

last: Mauldin

state: WA

address: 1100 Fairview Ave N D4-100

department: Human Biology

institute: Fred Hutchinson Cancer Research Center

description: A 5,285-feature mouse spotted cDNA microarray manufactured at the Fred Hutchinson Cancer Research Center comprised of mouse cDNAs obtained from the Research Genetics sequence-verified set of IMAGE clones.

manufacturer: Fred Hutchinson Cancer Center Genomics Resource

first: Peter

last: Nelson

last: Abbott

first: Colin

last: Pritchard

manufacture_protocol: Each microarray was comprised of 5,285 mouse cDNAs obtained from the Research Genetics sequence-verified set of IMAGE clones (cat. # 97002.MmV).  Clone inserts were amplified by PCR, purified, verified by gel electrophoresis and spotted onto poly-L-lysine-coated glass microscope slides using a GeneMachines robotic spotter.


title: SHES


title: SHEE


title: SHDS


title: Agilent Maize 21K v1.0

status: Public on Jan 01 2006

first: Jiong

last: Ma

address: 385 Serra Mall

institute: Stanford University

organism: Zea mays

description: 25x75 mm in dimension.

manufacture_protocol: 21K 60-mer oligo probes were designed from the MaizeGDB EST contigs and  synthesized in situ by an ink-jet printing method employing standard phosphoramidite chemistry on glass slides.


title: Mouse Full Powerblot Western Array (BD)

status: Public on Oct 20 2004

city: Haifa

first: Yotam

last: Sagi

address: 2 Efron st.

department: Department of Pharmacology , Faculty of Medicine

institute: Technion - Israel institute of Technology

technology: antibody

description:
The Full Poweblot Western Blotting Screening Service, Consisting of over 1,000 monoclonal Antibodies visualized using the Odyssey® Infrared Imaging System
Keywords = powerblot
Keywords = mAb

first: Sivia

last: Mandel

first: Moussa

last: Youdim


title: WUSTL Yersinia pestis 8.4k v.1

status: Public on Dec 16 2005

city: St. Louis

first: Seth

last: Crosby

state: MO

address: 660 S. Euclid

institute: Washington Univeristy School of Medicine

organism: Yersinia pestis

description: To construct a gene-specific microarray of the Y. pestis genome, we designed 70mer oligonucleotide array elements. Predicted Y. pestis genes, encoded on both the chromosome and the three plasmids of strain CO92, were obtained from the The Wellcome Trust Sanger Institute (http://www.sanger.ac.uk/Projects/Y_pestis/).  In addition genes derived from human, mouse, rat and virus were included to detect contaminating host mRNA.  Oligonucleotides were designed from these sequences using ArrayOligoSelector.

first: Wyndham

middle: W

last: Lathem

middle: D

first: Virginia

last: Miller

first: William

last: Goldman

manufacture_protocol: Oligos were synthesized using standard methods by Illumina (San Diego, CA). These oligonucleotides, as well as Ambion control E. coli sense oligos #7 and #8 (Ambion part# 1781), were dissolved at a concentration of 50 μM in 3X SSC with 0.75M betaine and were printed in duplicate on MWG Epoxy slides by a locally constructed linear servo arrayer (after the DeRisi model, http://derisilabs.ucsf.edu/).


title: BCM-NIA15k

status: Public on Sep 24 2004

city: Houston

first: Henning

last: Susan

state: TX

address: 1 Baylor Plaza

department: Pediatrics

institute: Baylor College of Medicine

description: BCM-NIA15k array

first: Agbernafle

last: Barbara

first: Oesterreicher

last: Thomas

first: Shaw

last: Chad


title: Center for Functional Analysis of Human Genome Human KUGI 14K (stomach cancer)

status: Public on Oct 06 2005

city: Daejeon

first: Heun-Sik

last: Lee

address: 52 Eoeun-dong, Yuseong-gu

institute: Korea Reaearch Institute of Bioscience and Biotechnology

manufacturer: The Center for Functional Analysis of Human Genome

manufacture_protocol: cDNA microarrays were fabricated in our core facility using a robotic arrayer (PixSYS 5500, Cartesian; Ann Arbor, MI). The 14K microarray contains 14,080 genes from KUGI unigene library (http://21cgenome.kribb.re.kr/html/2004_new/genome_php/cdna/cdna_list.htm). All the clones were verified by sequencing in our facility. The cDNA inserts were amplified by PCR, and after purification, spotted onto silylated glass slides (CSS-100; Telechem, Sunnyvale, CA). Each microarray slide contained control genes to normalize the effect of different fluorescent dyes.


title: PeterMac 8k cDNA chip

status: Public on Jul 05 2005

city: SG

first: Amit

last: Aggarwal

department: Cellular and Molecular Research

country: Singapore

institute: National Cancer Centre of Singapore

description:
8k cDNA chip
Keywords = Gastric Cancer

first: Alex

last: Boussioutas

first: David

last: Bowtell


title: 3168 rat cDNA array

status: Public on Dec 31 2003

city: Stockholm

first: Petra

middle: Elisabeth

last: Tollet-Egnell

address: CMM L8:01

department: Dept of Molecular Medicine

institute: Karolinska Institute

organism: Rattus norvegicus

description: 3168 rat cDNA array

first: Nina

last: Ståhlberg

first: Amilcar

last: Flores-Morales

first: Gunnar

last: Norstedt


title: ACG_SGF_CEA

status: Public on Mar 07 2003

city: Evry

first: Jerome

last: Lamartine

address: 2 rue Gaston Cremieux

department: Departement de Radiobiologie et Radiopathologie

country: France

description:
A collection of 2304 human genes corresponding to specific key-words was selected by direct interrogation of UniGene database. Primer pairs for the amplification of these 2304 human ORFs were obtained using the Primer 0.5 software. Specificity of each primer sequence was verified by blast analysis. The primers were designed to amplify a 400-600 bp product for each ORF. PCR amplification of each gene was performed using a mix of cDNAs obtained from various cell lines as template.
The PCR products were prepared in 96-well plates, purified by ethanol precipitation, washed in 70% ethanol, dried, dissolved in TE/DMSO (50/50) and stored at -80°C. PCR products were arrayed on amino-silane coated glass slide (Corning CMT-GAPS II) using a MicroGrid-II Micro-arrayer (BioRobotics, Cambridge, UK).
Keywords = cDNA array, PCR products

first: Pascal

last: Soularue

first: Amandine

last: Pitaval

first: Cyril

last: Petat

first: Olivier

last: Alibert

first: Nicolas

last: Ugolin

first: Sophie

last: Lemoine

first: Xavier

last: Gidrol


title: SHBQ

status: Public on May 25 2005

first: Xin

last: Chen

description:
SHBQ
Platform_coating: unknown_surface_type
Platform_distribution: non-commercial
Platform_manufacturer: I-Ping,,Tu; Stanford Functional Genomics Facility, CCSR 2255a, Stanford, CA, USA, 94305; 736-0077; iping@genome.stanford.edu
platform_support: glass
Platform_technology: other


title: SHBT

description:
SHBT
Platform_coating: unknown_surface_type
Platform_distribution: non-commercial
Platform_manufacturer: Joan,,Hebert; SFGF, 2255, CCSR, Stanford, Ca, USA, 94025; 650-736-0075; jhebert@genome.Stanford.edu
platform_support: glass
Platform_technology: other


title: ADQ_SGF_CEA

status: Public on Mar 05 2003

description:
A collection of 2304 human genes corresponding to specific key-words was selected by direct interrogation of UniGene database. Primer pairs for the amplification of these 2304 human ORFs were obtained using the Primer 0.5 software. Specificity of each primer sequence was verified by blast analysis. The primers were designed to amplify a 400-600 bp product for each ORF. PCR amplification of each gene was performed using a mix of cDNAs obtained from various cell lines as template.
The PCR products were prepared in 96-well plates, purified by ethanol precipitation, washed in 70% ethanol, dried, dissolved in TE/DMSO (50/50) and stored at -80°C. PCR products were arrayed on amino-silane coated glass slide (Corning CMT-GAPS II) using a MicroGrid-II Micro-arrayer (BioRobotics, Cambridge, UK).
Keywords = cDNA microarray PCR product

last: PItaval


title: SHBG

description:
SHBG
Platform_coating: unknown_surface_type
Platform_distribution: non-commercial
Platform_manufacturer: Joan,,Hebert; SFGF, 2255, CCSR, Stanford, Ca, USA, 94025; 650-736-0075; jhebert@genome.Stanford.edu
platform_support: glass
Platform_technology: other


title: ACX_SGF_CEA

description:
A collection of 2034 human genes corresponding to specific key-words was selected by direct interrogation of UniGene database. Primer pairs for the amplification of these 2304 human ORFs were obtained using the Primer 0.5 software. Specificity of each primer sequence was verified by blast analysis. The primers were designed to amplify a 400-600 bp product for each ORF. PCR amplification of each gene was performed using a mix of cDNAs obtained from various cell lines as template.
The PCR products were prepared in 96-well plates, purified by ethanol precipitation, washed in 70% ethanol, dried, dissolved in TE/DMSO (50/50) and stored at -80°C. PCR products were arrayed on amino-silane coated glass slide (Corning CMT-GAPS II) using a MicroGrid-II Micro-arrayer (BioRobotics, Cambridge, UK).


title: ADR_SGF_CEA

status: Public on Mar 06 2003

description:
A collection of 5760 full length cDNA clones from the human infant brain 1NIB library kindly gifted by Genethon as bacterial colonies in 96-well microtitre plates. These clones were characterized by 5' and 3' sequencing. Plasmids containing clones were grown in Escherichia coli by standard microbiological methods. Each insert was PCR amplified (30 cycles) by sampling 1 µl of cell culture with vector primers. To obtain a sufficient amount of probes, a second run of PCR, consisting of 20 cycles, was performed using the same set of primers. PCR products ranged from 600 to 4000 bp in length.
The PCR products were prepared in 96-well plates, purified by ethanol precipitation, washed in 70% ethanol, dried, dissolved in TE/DMSO (50/50) and stored at -80°C. PCR products were arrayed on amino-silane coated glass slide (Corning CMT-GAPS II) using a MicroGrid-II Micro-arrayer (BioRobotics, Cambridge, UK).
Keywords = cDNA array, PCR producrs


title: SHBF

description:
SHBF
Platform_coating: unknown_surface_type
Platform_distribution: non-commercial
Platform_manufacturer: Joan,,Hebert; SFGF, 2255, CCSR, Stanford, Ca, USA, 94025; 650-736-0075; jhebert@genome.Stanford.edu
platform_support: glass
Platform_technology: other


title: Atlas Rat cDNA Expression Array (Cat. #7738-1)

status: Public on Oct 01 2002

last: BD Biosciences Clontech

address: 1020 East Meadow Circle

institute: BD Biosciences Clontech

description: Nylon, broad-coverage rat cDNA array.  The cDNA fragments (200-600 bp) are PCR-amplified using gene-specific primers, and are selected so that they lack repetitive elements and have minimal homology with other genes on the array.


title: SHBD

description:
SHBD
Platform_coating: unknown_surface_type
Platform_distribution: non-commercial
Platform_manufacturer: Joan,,Hebert; SFGF, 2255, CCSR, Stanford, Ca, USA, 94025; 650-736-0075; jhebert@genome.Stanford.edu
platform_support: glass
Platform_technology: other


title: CCC-NB200-Chip-version1

status: Public on May 12 2005

city: Chiba

first: Akira

last: Nakagawara

address: 666-2 Nitona, Chuo-ku

institute: Chiba Cancer Center Research Institute

description:
Spotted long cDNA array on glass.
This set includes 200 cDNAs, whose average size is approximately 2.5kb.
Keywords = spotted by ink-jet printing, oligo-capping cDNA


title: Atlas Select Human Oncogene Array (Cat. #7831-1)

description: Contains genes shown to be differentially expressed in cell lines transformed by ErbB2 and N-Ras.


title: Atlas Rat Toxicology Array II (Cat. #7732-1)

description: Nylon, rat cDNA array designed to study toxicology.  The cDNA fragments (200-600 bp) are PCR-amplified using gene-specific primers, and are selected so that they lack repetitive elements and have minimal homology with other genes on the array.


title: Rat 6240 cDNA Chip

status: Public on Mar 22 2005

last: Flores

description:
A microarray containning 6240
rat probes. Probes are PCR amplified cDNA clones from multiple libraries.
Keywords = rat cDNA


title: CCC-NB5000-Chip-version1

description:
Spotted long cDNA array on glass.
This set includes 5340 cDNAs, whose average size is approximately 2.5kb.
Insert DNAs were amplified by PCR from the cDNA clones, purified by ethanol precipitation, and spotted onto a glass slide at a high density with an ink-jet printing tool.
Keywords = spotted by ink-jet printing, oligo-capping cDNA


title: Atlas Rat Toxicology 1.2 Array (Cat. #7860-1)

description:
Nylon, rat cDNA array designed to study toxicology.  The genes were assembled in collaboration with the Environmental Protection Agency (EPA).  The cDNA fragments (200-600 bp) are PCR-amplified using gene-specific primers, and are selected so that they lack repetitive elements and have minimal homology with other genes on the array.
er


title: SHDK

status: Public on Feb 09 2005

city: Stanford

last: Stanford Microarray Database

address: 300 Pasteur Drive

institute: Stanford Microarray Database (SMD)

description:
Manufacturer = Stanford Functional Genomics Facility,  Stanford School of Medicine
Platform_coating = unknown_surface_type
Platform_support = glass


title: PorkChip 2,600 cDNA array

status: Public on Oct 30 2004

city: St. Paul

first: Kendra

middle: A.

last: Hyland

state: MN

address: 1971 Commonwealth Ave.

department: Veterinary and Biomedical Sciences

institute: University of Minneosta

organism: Sus scrofa

description:
Spotted cDNA array on glass.
This set includes approximately 2,600 cDNA clones, selected from a Peyer’s patch subtracted library and other sources.
The PorkChip2 cDNA microarray contains 2,423 ESTs from the subtracted jejunal Peyer’s patch library (UMNMPM3), 10 ESTs from the normal jejunal Peyer’s patch library (UMNMPM1), and 26 ESTs from the immune-stimulated jejunal Peyer’s patch library (UMNMPM2), which were cloned using the GeneTrapper cDNA Positive Selection System and pCMVSport6 vector (Invitrogen Corporation, Carlsbad, CA) (2).  Each array also contains 86 ESTs identified by differential display RT-PCR (DD-RT-PCR) of PRRSV-infected macrophages (Library: PRRSV DD-RT-PCR)(5), 19 ESTs identified by DD-RT-PCR of influenza-infected porcine epithelial PK-15 cells (Library: UMNMPM4), 40 porcine genes, which were the result of directed cloning efforts (Library: Sus scrofa gene), and 7 porcine reproductive and respiratory syndrome virus strain VR-2332 sequences (Library: PRRSV) positive and negative controls (Library: Control).  Positive controls included 8 Ambion ArrayControl sequences. The plasmid inserts were amplified by two rounds of nested PCR using vector-specific primers.  The products from the second round PCR were cleaned by the Montage PCR(96) kit (Millipore, Billerica, MA) and were run on 0.8% agarose gels to quantify the amount of DNA by comparison to standards run on each gel.  The dried PCR products were resuspended in phosphate buffer (150 mM sodium phosphate and 1 mM EDTA (pH 8.5)) at a final concentration of 100-300 ng/microliter and transferred to 384 well plates. A BioRobotics MicroGrid II (BioRobotics Ltd., Haslingfield, Cambridge, UK) arrayed the DNA on slides coated with poly-L-lysine (Sigma, St. Louis, MO)(3).  Elements were arranged in 48 subarrays of 16 X 16, for a total of 12,288 spots per microarray, with each clone represented in by 4 spots.  The slides were baked for 15 min at 80 C, UV irradiated (65 mJ), and chemically blocked with 1-methyl-2-pyrrolidinone and succinic anhydride (4) and were stored at room temperature under vacuum until use.  The quality of each printing run was verified by staining one slide with SYBR Gold (Molecular Probes, Eugene, OR) and scanning at 488/530 nm (Excitation/ Emission) (1).
Platform_references: 1. Battaglia C, Salani G, Consolandi C, Bernardi LR, and De Bellis G. Analysis of DNA microarrays by non-destructive fluorescent staining using SYBR green II. Biotechniques 29: 78-81, 2000.  2. Dvorak CMT, Hyland KA, Machado JG, Zhang Y, Fahrenkrug SC, and Murtaugh MP. Gene discovery and expression profiling in porcine Peyer's patch. Vet Immunol Immunopathol, submitted, 2004.  3. Eisen MB and Brown O. DNA arrays for analysis of gene expression. Methods Enzymol 303: 179-205, 1999.  4. Shalon D, Smith SJ, and Brown PO. A DNA microarray system for analyzing complex DNA samples using two-color fluorescent probe hybridization. Genome Res 6: 639-645, 1996.  5. Zhang X, Shin J, Molitor TW, Schook LB, and Rutherford MS. Molecular responses of macrophages to porcine reproductive and respiratory syndrome virus infection. Virology 262: 152-162, 1999.
Keywords = small intestine, cDNA, pig

first: Cheryl

middle: M

last: Dvorak

first: Michael

last: Murtaugh


title: Incyte Human Gene Album Arrays 1-6

status: Public on Jan 15 2005

city: Kenilworth

first: Wei

last: Ding

state: NJ

address: 2015 Galloping Hill Road

department: Discovery Technology

institute: Schering Plough Research Institute

description: Incyte Human Gene Album Arrays 1-6

first: Qun

last: Wu

first: Asra

last: Mirza

first: Tish

last: Van Arsdale

first: Iris

last: Wei

first: Robert

last: Bishop

first: Andrea

last: Basso

first: Terri

last: McClanahan

first: Lin

last: Luo

first: Paul

last: Kirschmeier

first: Eric

last: Gustafson

first: Marco

last: Hernandez

first: Suxing

last: Liu


title: Atlas Human Hematology and Immunology Array (Cat. #7737-1)

last: BD Biosciences Clontech

description: Nylon, human cDNA array designed to study hematology and immunology.  The cDNA fragments (200-600 bp) are PCR-amplified using gene-specific primers, and are selected so that they lack repetitive elements and have minimal homology with other genes on the array.


title: Atlas Human Toxicology Array II (Cat. #7733-1)

description: Nylon, human cDNA array designed to study toxicology.  The cDNA fragments (200-600 bp) are PCR-amplified using gene-specific primers, and are selected so that they lack repetitive elements and have minimal homology with other genes on the array.


title: Atlas Human Cytokine and Receptor Array (Cat. #7744-1)

description: Nylon, human cDNA array designed to study cytokines and receptors.  The cDNA fragments (200-600 bp) are PCR-amplified using gene-specific primers, and are selected so that they lack repetitive elements and have minimal homology with other genes on the array.


title: Human pancreas-specific microarray

status: Public on Mar 02 2004

city: Lille

first: Brigitte

last: Vandewalle

address: 1 Place de Verdun

institute: Faculté de Médecine

description:
Human "in-house" pancreas-specific microarray
Keywords = human
Keywords = pancreas

first: Thomas

last: Bouckenooghe

first: Ericka

last: Moerman


title: Atlas Human cDNA Expression Array (Cat. #7740-1)

description: Nylon broad-coverage human cDNA array.  The cDNA fragments (200-600 bp) are PCR-amplified using gene-specific primers, and are selected so that they lack repetitive elements and have minimal homology with other genes on the array.


title: DKFZ Operon 27k long oligonucleotide array

status: Public on Aug 04 2004

city: Heidelberg

first: Joerg

last: Schlingemann

address: Technologiepark III, Im Neuenheimer Feld 280

department: Molecular Biology (B060)

institute: German Cancer Research Center (DKFZ)

description:
Spotted long oligonucleotide array produced using the Operon Human Genome Oligo Set Version 2.1  (21,329 70mer oligonucleotides, based on build 147 of the Human UniGene database and the Human RefSeq Database, plus 24 controls) and the Operon Human Genome Oligo Set Version 2.1 Upgrade (5,462 70mer oligonucleotides, based on build 13.31 of the Human Ensembl Database). Spotting was performed in duplicates (replicate arrays) with 4x6 SMP3 pins (Telechem) at 18-20 °C and 40-50% RH on epoxysilane coated slides (Quantifoil) using 40 µM oligos in FBNC spotting buffer  (G. Wrobel et al., Nucleic Acids Res 31 e67, 2003). Spot centers were 129 µm apart. DNA adhesion was accomplished by 1 h incubation at 60 °C followed by 240 mJ/cm2 UV irradiation at 254 nm.
Keywords = spotted
Keywords = human
Keywords = long oligonucleotide
Keywords = oligo
Keywords = 70mer
Keywords = Operon

first: Olaf

last: Thuerigen

first: Grischa

last: Toedt

first: Heidi

last: Kramer

first: Meinhard

last: Hahn

first: Peter

last: Lichter


title: Abeta-activated Microglia Part 2

status: Public on Oct 29 2003

city: South San Francisco

first: Scott

middle: Curtis

last: Lohr

address: 290 Utah Ave.

department: Bioinformatics

institute: AGY Therapeutics Inc.

description:
RNA Preparation
Total RNA from abeta stimulated cells was isolated using Tri Star reagent according to the manufacturer's instructions (Invitrogen, Carlsbad, CA).  The resulting RNA was treated with RNAse free DNAse (Ambion, Austin, Texas) for 40 minutes at 37.C.  Isolation of mRNA was carried out using Oligotex (Qiagen, Chatsworth, CA).
Library Generation
To construct the subtractive and normalized cDNA libraries, reverse transcription and second strand synthesis were performed using the Superscript II reverse transcription kit (Invitrogen, Carlsbad, CA) with an anchored Oligo dT (30) primer. Subtractive and normalized cDNA libraries were generated using suppression PCR.
Array Creation
Subtracted and normalized cDNA libraries were cloned into the pCR2.1 vector (Invitrogen, Carlsbad, CA), and plated. Single colonies were picked into 384 well plates using an automated colony picker (Q-Pix, Genetix, Cambridge, UK) to grow overnight and subsequently serve as templates for PCR. PCR products were spotted on Nylon membranes using a 384 pin head arrayer (Q-bot, Genetix, Cambridge, UK). The spotted PCR product on all filters were cross-linked to the membrane by UV light. Filters were neutralized and denatured using standard protocols.
Library Description
SL00099:12h abeta stimulated
SL00100:18h abeta stimulated
SL00101:24h abeta stimulated
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last: Gan

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last: Ye

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last: Vincent

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last: von Schack

last: Chin

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last: Murray

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last: Gonzalez-Zulueta

last: Nikolich

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title: Atlas Glass Mouse 1.0 Array (Cat. #7901-1)

city: Palo Alto

last: BD Biosciences Clontech

description: Glass microarrays made with long (~80 bp) experimentally-tested oligonucleotides.