title: Alternative Human Exon 1.0 ST Array [custom pri-miRNA meta-probeset mappings]

status: Public on Jan 16 2009

city: Bolzano

first: Johannes

last: Rainer

address: Via A. Volta 21

department: Institute for Biomedicine

country: Italy

institute: Eurac Researc

organism: Homo sapiens

technology: in situ oligonucleotide

description: The probe set groupings in the below table were custom-derived based on Affymetrix Netaffx 24 (November 2007). The pre-miRNA-sequences correspond to Sanger miRBase version 10.1

distribution: custom-commercial

manufacture_protocol: See manufacturer's web site


title: New Zealand White Rabbit Heart 2.8K cDNA

status: Public on Jan 28 2009

city: Boston

first: James

middle: D

last: McCully

state: MA

address: 77 Avenue Louis Pasteur

department: Cardiothoracic Surgery

country: USA

institute: BIDMC/Harvard Medical School

organism: Oryctolagus cuniculus

technology: spotted DNA/cDNA

distribution: non-commercial

manufacture_protocol:
cDNA Library : Isolation of  total heart (atria and ventricles)RNA:  RNA was isolated  from New Zealand rabbit (15-20 weeks; 3 to 4 kg) heart in an RNase-free environment using RNase-free lab-wear, materials and equipment according to the  procedure as described by Chomczynski and Sacchi (Anal Biochem 162:156-15). In brief tissue was pulverized under liquid nitrogen using  mortar and pestle and extracted two times in acidic guanidinium isothiocyanate-phenol-chloroform and incubated for 5 min. at room temperature to permit the complete dissociation of nucleoprotein complexes. The mixture was centrifuged at 14,000x g for 15 min. at 4oC. Following centrifugation, the aqueous phase containing the RNA was carefully removed and transferred to a new tube containing 0.5 mL isopropanol and incubated for 10 min. at room temperature to precipitate the RNA. The RNA was collected by centrifugation at 14,000x g for 20 min. at 4oC. The RNA pellet washed with 1 mL of room temperature 75%  ethanol, re-centrifuged and the RNA pellet air-dried and dissolved in DEPC-treated H2O.
POLY(A)+ RNA fractions were enriched by oligo(dT) cellulose chromatography. In brief, 1 mg of  total RNA was adjusted to 0.55 mg/mL RNA concentration in DEPC-treated H2O, and incubated for 5 min. at 650C and then chilled on ice.  The salt concentration was adjusted to 0.5M NaCl and the RNA sample mixed with 1 mL of oligo(dT) cellulose. The  suspension was  incubated for 10 min. at 37oC to allow the mRNA to bind to the oligo(dT) cellulose. The unbound RNA was eluted  and re-heated for 5 min. at 65 oC, chilled on ice and re-suspended in the oligo(dT) cellulose. The unbound RNA was washed from the column with a low salt buffer and the bound mRNA eluted from the oligo(dT) cellulose beads with 1 mL H2O (65 oC). The eluted mRNA was re-heated for 5 min. at 65 oC, chilled on ice and re-suspended in the oligo(dT) cellulose and then eluted as described above in order to further reduce any rRNA contamination. Fractions (200 uL) was collected in microfuge tubes. Positive fractions containing mRNA were determined by UV spectrophotometric analysis, pooled and the mRNA precipitated overnight, at -20oC with 50 ug/mL glycogen, 0.1 volume 7.5 M ammonium acetate and 2 volumes ethanol (-20 oC) and then collected by centrifugation  at 14,000x g for 30 min. at 4 oC. The mRNA pellet was washed twice with 75% ethanol, air-dried and dissolved in DEPC-treated H2O. The mRNA yield was determined by UV spectrophotometric analysis (260 nm) with the quality of the mRNA determined by gel electrophoresis on a 1% agarose gel containing 3% formaldehyde, 0.02 M MOPS pH 7.4 (3-[N-morpholino] propanesulfonic acid) and 0.001 M disodium EDTA (ethylenediamine tetraacetic acid) .
cDNA LIBRARY CONSTRUCTION:  cDNA libraries were constructed  using the  SuperScript Plasmid System (GibcoBRL, cat. No.18248-013) according to the manufacturer’s instructions.  The cDNAs were ligated into the plasmid vector pSPORT 1  (4,109 bp) and then transformed into DH5 competent cells (GibcoBRL, Cat. No.10643-013) according to the manufacturer’s instructions.
Estimation of cDNA library size was performed by titration of transformed  bacteria on LB-agar plates (1% bacto-tryptone, 0.5% bacto-yeast extract, 1% NaCl, 1.5% bacto-agar)containing IPTG/X-gal (0.02 ug/mL each)and ampicillin (75 ug/mL). Aliquots of cDNA libraries were stored in 7% DMSO at -80oC.
Estimation of cDNA insert size was determined by random selection of cDNA clones from IPTG/X-gal LB-agar plates containing ampicillin. In brief, single isolated cDNA clones were selected and used to inoculate 2 mL LB liquid media (1% bacto-tryptone, 0.5% bacto-yeast extract, 1% NaCl) containing ampicillin (75 ug/mL) and  incubated overnight at 500 rpm at 37oC in a New Brunswick incubator. The recombinant bacteria werre chilled on ice and the recombinant bacterial pellet collected by brief centrifugation. Plasmid cDNA was isolated by alkaline lysis using  Qiagen columns (cat. No. 12125) and the plasmid DNA precipitated with 0.6 volumes isopropanol by centrifugation at 14,000x g for 30 min. The cDNA pellet was washed twice with 70% ethanol, air-dried and dissolved in TE buffer (10 mM Tris-HCl, pH 8.0; 0.1 mM EDTA, pH 8.0). The purified plasmid cDNA was digested for 1 hour at 37oC in a reaction mixture containing a 3-fold excess of the restriction enzyme Mlu I (New England Biolabs, Cat. No. R0198S) which cleaves the unique restriction enzyme sites located at the termini of the cDNA insert distal to the  Not I and Sal I insertion sites. The digested cDNA was resolved on a 1% agarose gel in 40 mM Tris-acetate and 2 mM EDTA  (TAE) and the gel stained with ethidium bromide then photographed under UV illumination. DNA molecular weight markers (1 kb; New England Biolabs; 323-2S)  were used for estimation cDNA molecular weights.
Isolation and growth:  cDNA libraries were plated at low density  on  IPTG/X-gal LB-agar plates containing ampicillin (75 ug/mL) and  incubated overnight at 37oC.  The following day cDNA clones were selected, individually and used to inoculate individual wells in 96-well culture blocks (USA Scientific, Cat. No.7556-9600) containing 1.3 mL/well liquid LB media containing ampicillin (75ug/mL) and the plates oriented and  labeled to indicate clonal position and then sealed with Breathe–Easy sealing film (USA Scientific) and incubated overnight at 500 rpm at 37oC in a New Brunswick incubator.
Replicate plates were produced following overnight incubation by removing 100 uL samples from each well in 96-well culture blocks for the  inoculation of replicate wells in 96-well culture blocks containing 0.9 mL/well liquid LB media containing ampicillin (75 ug/mL). The replicate plates were oriented, labeled in the same manner as above, sealed with Breathe–Easy sealing film (USA Scientific) and incubated overnight at 500 rpm at 37oC in a New Brunswick incubator. Following overnight incubation replicate plates were cooled on ice and glycerol added to a  final concentration of 20 % /well and the plates sealed with  Thermowell sealers (Costar 6524) and stored at –80oC.
5’ Sequencing:  Plasmid cDNA were isolated by Qiagen Bio Robot 8000 automated nucleic acid purification and liquid handling workstation available for use by our laboratory through core facilities. Purified cDNAs were sequenced using the Applied Biosystems Taq DyeDeoxy Terminator cycle sequencing kit which utilizes a fluorescently-labeled dideoxy-nucleotide chain termination method. After cycle sequencing and clean up the DNA samples were resolved by capillary electrophoresis on an PE BIOSYS 3700 automated sequencer which translates the fluorescent signals into their corresponding base pair sequence.
Sequence  analysis of all cDNAs was performed against the non-redundant GEN Bank/ EMBL/ DDBJ nucleotide, the non-redundant Gen Bank CDS translation/ PDB/ Swiss  Prot/ PIR/ PRF peptide and the dbEST databases using the BLAST algorithm on a UNIX platform. Assignment of putative identity for cDNAs exhibiting matches to known genes required a maximum value of E=1e-25 (minimum p=10-10)and nucleotide sequence identity >95%. Prior to analysis of sequence data, sequence quality were evaluated using the accuracy assessment program  phred of the phred/phrap/consed assembly package. All sequence traces from the  ABI model sequencer werebe saved in fasta output format.
Sequence submission and storage: Sequence quality was evaluated using the accuracy assessment program  phred of the phred/phrap/consed assembly package. All sequence traces were saved in fasta output format. Sequence analysis was performed against the non-redundant GEN Bank/ EMBL/ DDBJ nucleotide, the non-redundant Gen Bank CDS translation/ PDB/ Swiss  Prot/ PIR/ PRF peptide and the dbEST databases using the BLAST algorithm on a UNIX platform . All cDNA’s were 5’ sequenced and are available under accession numbers EC618425-EC626095 and  dbEST40150263-40157933 and at www.mccull


title: MPIP24K

status: Public on Jan 31 2009

city: München-Neuherberg

first: Dietrich

last: Trümbach

address: Ingolstädter Landstraße 1

department: Institute for Developmental Genetics

country: Germany

institute: German Research Center for Environmental Health

organism: Mus musculus

manufacture_protocol: The cDNA microarray contains 24192 PCR products derived from different mouse cDNA libraries (sources: Research Genetics, RZPD Deutsches Ressourcenzentrum für Genomforschung, proprietary clones) representing 12387 different unigene clusters (unigene build #143). The spotting buffer contained 3 x SSC and 1.5 M betaine. The PCR products were spotted on GAPS II slides (Corning) using a Chipwriter Pro spotting robot (Biorad) and 48 SMP3 stealth pins (Telechem). After spotting, the arrays were heated to 80°C for 10 seconds and crosslinked using a UV Stratalinker 2400 at 200mJ (Stratagene). Slides were blocked 30 min at 65°C and prehybridized for 1 h at 42°C in mixture with 0.1 mg/ml BSA, 50% formamide, 5 x SSC and 0.1 x SDS. The arrays were rinsed with ultrapure water, then vigourously shaken for a few seconds in a 50 ml polypropylene tube containing isopropanol. The arrays were dried by centrifugation for 2 minutes at 400 g and hybridized within a few days.


title: Princeton Lewis-Sigler Institute Plasmodium 8.5K array version 1

status: Public on Jan 24 2009

city: Princeton

first: Kellen

last: Olszewski

state: NJ

address: Carl Icahn Lab

department: Molecular Biology

institute: Princeton University

organism: Plasmodium falciparum

technology: spotted oligonucleotide

description: Llinas lab print 8-18-2006

manufacturer: Lewis-Sigler Institute Microarray Facility

manufacture_protocol:
Printer:	Arrayer 250A
Array Configuration: (columns X rows)	32 sectors ( 4 X 8 spotted) Standard 32-tip
Spot Columns per Sector: 24
Spot Rows per Sector: 11
Column Spacing: 178
Row Spacing: 178


title: MIE Plasmodium berghei 22k Microarray

status: Public on Jan 13 2009

city: tsu

first: masao

last: yuda

state: mie

address: edobashi 2-174

country: Japan

institute: mie university

organism: Plasmodium berghei

first: Shuji

last: Shigenobu

manufacture_protocol: see manufacture's website


title: NimbleGen Homo sapiens custom tiling (2006-07-20_Flanagan_HG17_ChIP)

status: Public on Jan 29 2009

city: London

last: Flanagan

address: Du Cane Rd

department: Dept of Oncology

country: United Kingdom

institute: Imperial College London

manufacture_protocol: see the manufacturer's website


title: DASL Human 1.5K Cancer Panel

status: Public on Jan 30 2009

city: Brisbane

first: Leonard

last: Da Silva

address: Herston Road

country: Australia

institute: University of Queensland

technology: oligonucleotide beads

manufacture_protocol:
The cDNA-mediated Annealing, Selection, Extension, and Ligation (DASL) Assay is designed for RNA profiling of up to 1,536 targets using partially degraded RNA as found in formalin-fixed, paraffin-embedded samples.
The core of the experiment relies on beadarray technology - Illumina’s BeadArray Technology is based on 3-micron silica beads that self assemble in microwells on either of two substrates: fiber optic bundles or planar silica slides. When randomly assembled on one of these two substrates, the beads have a uniform spacing of ~5.7 microns. Each bead is covered with hundreds of thousands of copies of a specific oligonucleotide that act as the capture sequences in one of Illumina’s assays. Because the beads are positioned randomly, a decoding process is carried out to determine the location and identity of each bead in every array location. Decoding is an automated part of array manufacture - extracted from www.illumina.com.
Assay Probe Design: As described by Bibikova et al Am J Pathol. 2004 November; 165(5): 1799–1807  - "Two probe oligonucleotides are designed to interrogate each target site on the cDNA, with 3 to 10 target sites per gene (average 6 sites). The first oligo consists of two parts: the gene-specific sequence and a universal PCR primer sequence (P1, 5′-ACTTCGTCAGTAACGGAC-3′) at the 5′-end. The second oligo consists of three parts: the gene-specific sequence, a unique address sequence which is complementary to one of 1520 capture sequences on the array, and a universal PCR primer sequence (P2, 5′-GTCTGCCTATAGTGAGTC-3′) at the 3′-end. A single address sequence is uniquely associated with a single target site. This address sequence allows the PCR-amplified products to hybridize to a universal microarray bearing the complementary probe sequences. The gene-specific sequence is designed with Tm ranging from 57°C to 62°C."


title: Nimblegen human 16MB custom tiling array (HG17) design2

status: Public on Jan 15 2009

city: Toronto

first: Zhaodong

last: Xu

state: Ontario

address: Mc6-424, 399 Bathurst Street

department: Genetics & Development Division

country: Canada

institute: TWRI, UHN

first: Rod

last: Bremner

first: Tao

last: Yu

manufacture_protocol: as descrbed in www.nimblegen.com


title: Nimblegen human 16MB custom tiling array (HG17) design1


title: Conway Institute Candida parapsilosis 5834 v1.0

city: Dublin

first: Geraldine

last: Butler

address: Belfield

department: School of Biomolecular and Biomedical Science

country: Ireland

institute: University Colege Dublin Conway Institute

organism: Candida parapsilosis

description: design no 015742

first: Alessandro

last: Guida

manufacture_protocol: Oligonucleotides were designed from an in-house annotation of the C.parapsilosis genome sequence (http://www.sanger.ac.uk/sequencing/Candida/parapsilosis/) using eArray from Agilent Technology. Each of 5834 open reading frames is represented by two oligonucleotides probes. Mitochondrial genes are indicated by the prefix "cpmt". Controls from Arabidopsis thaliana were included. The probes were used to generate custom microarrays in a 4x44 K format with a randomized layout.


title: Alternative Human Exon 1.0 ST Array [custom pre-miRNA probe annotations]

description: Affymetrix Human Exon 1.0 ST Array probe sequences where aligned with pre-miRNA sequences from the sanger miRBase (version 10.1). All probes with 100% sequence matches to the same pre-miRNAs are defined as probeset to detect the specific pre-miRNA.


title: SMD Vibrio cholerae Print_356

status: Public on Jan 21 2009

city: Stanford

last: Stanford Microarray Database

state: CA

address: 300 Pasteur Drive

department: Stanford University, School of Medicine

institute: Stanford Microarray Database (SMD)

organism: Vibrio cholerae

manufacturer: Stanford Functional Genomics Facility, Stanford School of Medicine

manufacture_protocol: Material is transferred from microtiter plate wells to the slide surface by an automated print head


title: DiFazio_Poplar_60mer_NGD

city: corvallis

first: palitha

last: dharmawardhana

state: OR

address: 321 richarson hall,OSU

institute: OSU

organism: Populus trichocarpa

description: This array has probes designed from gene models taken from the Joint Genome Institute Populus trichocarpa genome sequence, as well as probes designed using aspen ESTs.

first: S

middle: P

last: DiFazio

first: A

middle: M

last: Brunner

first: P

last: Dharmawardhana

first: K

middle: J

last: Munn

manufacture_protocol: Propriety in situ oligonucleotide synthesis.


title: Surrey Streptomyces coelicolor array for analysis of two component signal transduction systems

city: Taipei

first: Ralph

last: Kirby

address: Li-Nong Road

country: Taiwan

institute: National Yang-Ming University

organism: Streptomyces coelicolor A3(2)


title: Human RefSet oligo 24k array

city: Cambridge

first: Andrew

last: Sharkey

address: Tennis Court Rd

department: Pathology

institute: University of Cambridge

manufacturer: University of Cambridge, Pathology department, Centre for Microarray Resources

first: Ariane

last: Germeyer

first: Ashley

last: Moffett

manufacture_protocol: The human RefSet oligo array is built with an open source probe collection designed to yield information about ~21,000 human genes. The collection includes ~16,000 probes designed to ~12,600 single-isoform and 1,300 multiple-isoform curated RefSeq genes, and a further ~6,800 probes for validated RefSeq genome annotations. Further information can be found at http://www.illumina.com/products/dna/genomesets/reft_set_oligos.ilmn. The 23,000 70mers is a single array built on a background-reduced Corning UltraGAPS surface. Spotted with Pins.


title: Agilent_ENCODE_44K_HG18_custom

status: Public on Jan 23 2009

city: Cleveland

first: Mike

middle: Paul

last: Schnetz

state: OH

address: 10900 Eulid Ave

department: Genetics

institute: Case Western Reserve University

description: 4-plex microarray with each array containing features tiling across 6 ENCODE regions (HG18)

first: Peter

middle: C

last: Scacheri

manufacture_protocol: see manufacturer's web site at http://www.agilent.com/


title: University of Vermont Affymetrix Streptococcus mutans UA159 3K microarray version 1

status: Public on Jan 18 2009

city: Burlington

first: Jeffrey

last: Bond

state: VT

address: 95 Carrigan Dri e

department: Microbiology and Molecular Genetics

institute: University of Vermont

organism: Streptococcus mutans UA159

manufacture_protocol: Affymetrix Microchip Manufacturing Protocol


title: Steroltalk v2 human

city: Ljubljana

last: Juvan

address: Zaloska 4

department: Institute of Biochemistry

country: Slovenia

institute: Faculty of Medicine, University of Ljubljana

manufacturer: Center for Functional Genomics & Bio-Chips, Faculty of Medicine, Univ. of Ljubljana, Slovenia; Lek Pharmaceuticals d.d., Ljubljana, Slovenia; Genome Scale Biology, Biozentrum, Univ. of Basel, Basel, Switzerland; GODMAP, CGM, CNRS, Gif-sur-Yvette, France

first: Tadeja

last: Rezen

first: Damjana

last: Rozman

first: Klementina

last: Fon Tacer

first: Drago

last: Kuzman

first: Uros

last: Urleb

first: Adrian

last: Roth

first: Urs

middle: A

last: Meyer

first: Herve

last: Delacroix

first: Nicolas

last: Agier

first: Lawrence

last: Aggerbeck

first: Denis

last: Pompon

first: Sandrine

last: Imbeaud

manufacture_protocol:
Spotter: Biorobotics Microgrid II
Number of pins: 2
Probes: cDNA Slides: Corning Gaps II
Number of probes (for each array): 304 human in 3 replicas, 528 control
Number of blocs: 6 per array, 2 arrays per slide
Block size: 16x15 spots (height x width)
Pitch between spots: 260 µm
Spot size: between 110 and 170 µm
Printed: 55 slides (110 chips)
Process length: ~ 19H
Humidity: 40% in the room
Temperature: 20°C+/-0.5
Buffer conditions: formamide 50%, Chaps 1%


title: Invitrogen NCode Multi-Species miRBase Microarray V2

city: Gaithersburg

first: Sriram

last: Sridhar

state: MD

address: 1 MedImmune Way

department: Translational Medicine

institute: MedImmune

organism:
Caenorhabditis elegans
Drosophila melanogaster
Danio rerio
Homo sapiens
Mus musculus
Rattus norvegicus

manufacture_protocol: The NCode™ Multi-Species miRBase Microarray Kit V2 contains optimized probes targeting all of the known mature miRBases in mirBase 9.0 for human, mouse, rat, Drosophila, C. elegans, and zebrafish, spotted in triplicate on Corning Glymo epoxide slides


title: Nimblegen custom Haemophilus influenzae multi-genome array

city: Oklahoma City

first: Timothy

last: VanWagoner

state: OK

address: 940 SL Young Blvd, BMS340

department: Pediatrics

institute: University of Oklahoma Health Sciences Center

organism: Haemophilus influenzae

description: The Platform data table is a condensed representation of the array's replicate features.  The full array layout representing all of the individual features is linked as a supplementary file at the foot of this record.  PROBE_SEQUENCE information can be found within the full array layout file.

first: Paul

middle: W

last: Whitby

first: Thomas

last: Seale

first: Daniel

last: Morton

first: Terrence

middle: L

last: Stull

manufacture_protocol:
Oligonucleotide arrays were synthesized by NimbleGen Systems, Inc by maskless array synthesis using a digital micromirror array-mediated, parallel synthesis process incorporating 5’-photoprotected phosphoramidites as previously described. (1) Synthesized arrays were deprotected by incubation in ethanolamine:ethanol (1:1) at room temperature for two hours, washed extensively in distilled water, and stored desiccated until used.
Expression design for 4 strains of H. influenza with 13 probes/gene.  Expression probe set replicated 3x on chip.  All intergenic regions tiled on both strands at a 15bp spacing


title: Myxococcus xanthus 7.2K

city: Syracuse

first: Anthony

last: Garza

state: NY

address: 212 BRL,  130 College Place

department: Biology

institute: Syracuse University

organism: Myxococcus xanthus

description:
PCR generated DNA microarrays containing probes to the 7,235 M. xanthus ORFs identified on the M1genome M. xanthus (Jakobsen et al., 2004).
spotted double stranded DNA (PCR product)

manufacturer: Stanford Functional Genomics Facility (Stanford CA) in conjunction with The Myxococcus Microarray Consortium.

manufacture_protocol:
1.PCR products are arrayed in Greiner 384-well flat-bottom plates. Each well contains 600 pmol of DNA
2. Resuspend DNA in water to 20 uM and rearray 5 µL into 384-well, Genetix polystyrene V-bottom plates (cat# X6004).
3. Allow Genetix plates to dry through passive water evaporation in a protected environment (e.g., chemical hood).
4. Before printing, add 5 µL of 1X Printing Buffer to each well. This can be done the night before a print run is started.
5. Seal plates with Corning seals.
6. Incubate at 37°C for 30 minutes to aid resuspension of DNA.
7. Shake plates near maximum rotational speed on flat-bed shaker for 1 minute.
8. Centrifuge plates at 2000 rpm for 3 minutes.
9. Remove seals and cover with plate lids. Place in appropriate location of plate cassette. This should be done with first plates just before print run is started to minimize evaporation time before printing. For second and third cassettes, wait until 30
10. Make sure plates rest behind both holding clips in the cassettes. Push plates back into the cassettes as far as they will go, putting them in the proper position for the server arm.
11. After the print run is completed, allow plates to dry through passive evaporation in a protected environment.
12. For each subsequent preparation of these plates for a print run, add water to the wells instead of sodium phosphate buffer. The amount of water should be decreased by 0.25 µL per print run, as this is the amount drawn up by the pin capillary during ea


title: Cotton Array Version 2

status: Public on Jan 14 2009

city: New Orleans

first: Doug

last: Hinchliffe

state: LA

address: 1100 Robert E Lee Blvd

department: Southern Regional Research Center

institute: USDA-ARS

organism:
Gossypium hirsutum
Gossypium arboreum
Gossypium raimondii

manufacturer: In-house print with additional features described in the matrix file

manufacture_protocol: The in-house print of the Cotton Array Version 2 was supplied by Dr. Z. Jeffrey Chen, The University of Texas at Austin, Institute for Cellular and Molecular Biology


title: Porcine oligo micro array version 3

city: Copenhagen

first: Shila

last: Mortensen

address: Bülowsvej 27

department: Dep. for Veterinary Diagnostics and Research

country: Denmark

organism: Sus scrofa

manufacturer: National Veterinary Institute, Technical University of Denmark.

first: Kerstin

last: Skovgaard

first: Peter

last: Heegaard

first: Mette

last: Boye

manufacture_protocol:
The porcine oligo microarray version 3 (POM3) contains 192 porcine oligoes of maximum 75mer, targeting annotated mainly immune related porcine transcripts, a number of negative controls and spike in (Ambion).
The porcine target sequences used for oligo design were obtained from public databases (NCBI, National Center for Biotechnology Information, GenBank (http://www.ncbi.nlm.nih.gov/) and TIGR, Institute for Genome Research, http://www.tigr.org/).
Oligo design was performed using the microarray probe design software Oligowiz (Nielsen HB, Wernersson R and Knudsen S. Design of oligonucleotides for microarrays and perspectives for design of multi-transcriptome arrays. Nucleic Acids Res 31: 3491-3496, 2003)(http://www.cbs.dtu.dk/services/OligoWiz2/), using the default settings, except for the oligo length, where longer oligos were selected.
Oligos were synthesized and 5’ aminoallyl-conjugated by DNA Technology A/S (Århus, Denmark).
Oligoes were diluted to 50mM in 50% DMSO -and array controls were diluted to 30mM in 50% DMSO. Oligoes were spottet on Corning UltraGAPSTM Coated Slides (Corning B.V.Life Sciences, Schiphol-Rijk. The Netherlands) using a VersArray ChipWriterTM Pro, software version 2.1.1 (Bio-Rad Laboratories Ltd. Ontario, Canada) equipped with 4 SMP3 pins (TeleChem International, Inc., Sunnyvale, CA).
Oligos were spotted in triplicate in 4 sub-arrays each holding 12 x 15 spots and the 4 sub-arrays were repeated 3 times. After printing, slides were dried, UV cross-linked and stored in vacuum dessicator at room temperature until use. Prior to hybridization the slides were pre-hybridized according to manufacturer’s instruction.


title: Agilent-021784 Synechocystis_predictions_tiling_28nt_105K

city: Freiburg

first: Jan

last: Mitschke

address: Schaenzlestr. 1

department: Biologie III

institute: University of Freiburg

organism: Synechocystis sp. PCC 6803

manufacture_protocol: See manufacturer's web site at http://www.agilent.com/


title: KTH_E.Coli_6k_Oligo_Microarray

city: Tallinn

first: Ranno

last: Nahku

address: Akadeemia tee 15b

country: Estonia

institute: TFTAK

organism: Escherichia coli

manufacturer: KTH Microarray Center, Sweden (Stockholm)

manufacture_protocol:
The E. coli Genome Oligo Set Version 1.0 consists of 5978 70mer probes representing tree strains of Escherichia coli. The number of open reading frames (ORFs) represented is as follows: 4289 ORFs in K12; 5059 ORFs in O157:H7(EDL933); 4836 ORFs in O157:H7Sakai; All 5978 probes are designed within ORFs from both cloned genes and predicted ORFs. For our probe design, we use state-of-the-art methods and proprietary software.
All probes are Tm normalized to 75ºC (±5ºC) and have a 5' amino linker.
http://www.operon.com/arrays/oligosets_ecoli.php

Array manufacturing
The 5978 70mer probes were dissolved in Universal Spotting Solution (Corning) to a concentration of 18 µM. The microarrays were printed with a QArray (Genetix) instrument with 24 PT2500 pins (Point Technologies) on Ultra GAPS slides (Corning). The 5978 70mer probes were spotted in a 17x17 pattern within each block and with a feature center-to-center distance of 250 µm in two identical fields. The quality of the spotted slides was assessed by staining with Syto61 (Molecular Probes) and with hybridization with random nonamers. The slides were UV cross-linked at 150 mJ/cm2.


title: Custom human (hg17) imprinting tiling array

city: Baltimore

first: Andrew

middle: P.

last: Feinberg

address: 855 N. Wolfe Street

department: Center for Epigenetics and Department of Medicine

institute: Johns Hopkins University

organism: Homo sapiens

manufacture_protocol: Human genome tiling arrays containing imprinted regions


title: Saccharomyces cerevisiae 10.9K Print_AG3

city: Zürich

first: Bernahrd

last: Dichtl

address: Winterthurer Strasse 190

department: Institute of Molecular Biology

country: Switzerland

institute: University of Zürich

organism: Saccharomyces cerevisiae

description: Identical to platform GPL7663

manufacturer: Center for Integrative Genomics, University of Lausanne, Switzerland

first: André

last: Gerber

manufacture_protocol: 70-mer oligos comprising the Array-Ready Oligo Set Version 1.1, and the Yeast Brown Lab Oligo Extension Version (YBOX vers. 1.0) from Operon were transferred from microtiter plate wells to the slide surface by an automated print head


title: Agilent_Dm_4.3_612_tiling

city: Durham

first: David

last: MacAlpine

state: NC

address: Box 3813 DUMC

department: Pharmacology and Cancer Biology

institute: Duke University Medical Center

organism: Drosophila melanogaster

description: CGH resolution tiling array for modENCODE. Average probe spacing of 612 bp for Drosophila genome (4.3).

manufacture_protocol: Agilent Technologies


title: ExonHit Human Genome Wide SpliceArray 1.0

first: Michael

middle: Ian

last: Brenner

address: 217 Perry Parkway bld 5

institute: ExonHit Therapeutics

description:
The Human Genome Wide Splice Array 1.0 contains over six million oligonucleotides designed to monitor expression of 20,649 genes and interrogate evidenced and predicted alternative splicing events.
Alternative splicing events were identified by comparing the exon structure of a selected representative sequence from a gene to the putative exon structure of overlapping and related public sequences (including RefSeq, mRNA and EST databases from GenBank). Probes were designed to monitor the alternative isoforms indicated.

distribution: commercial

manufacture_protocol: The Hs GWSA 1.0 is manufactured on the Affymetrix 49-format GeneChip with 5 micron feature size. Please see Affymetrix website for additional manufacturing protocol information.


title: Custom NimbleGen rat tiling array [2007-03-26_RN34_TK_ChIP]

city: Boston

first: Tae-Kyung

last: Kim

address: 300 Longwood avenue, CLS building 12260

department: Neurology

institute: Children's Hospital

organism: Rattus norvegicus

manufacture_protocol: see NimbleGen website


title: Agilent_Dm_5.1_470_tiling

description: CGH resolution tiling array for modENCODE. Average probe spacing of 470 bp for Drosophila genome (5.1) and unique heterochromatin scaffolds.


title: MacLeod custom equine cartilage cDNA microarray, Version 2

status: Public on Jan 08 2009

city: Lexington

middle: J.

last: Mienaltowski

email: M.Mski@uky.edu, mmienalt@health.usf.edu

state: KY

address: 108 Gluck Equine Research Center

department: Veterinary Science

institute: University of Kentucky

organism: Equus caballus

description: The 9,322 cDNAs spotted on this microarray were obtained from a normalized and subtracted cDNA library generated from the total RNA of articular cartilage from a 15-month old equine Thoroughbred gelding. In addition, cDNAs from 28 plasmids used within the lab were spotted; 2 plasmids have been submitted to GenBank. Positive control spots include Elongation Factor Tu (EFTu) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Negative control spots are Invitrogen oligos from Arabidopsis thaliana genes CAD1 and GAPC.  The original negative control soybean genes chlorophyll ab binding protein (CAB - SPOT_ID: ABbinding) and Rubisco small chain 1 (RBS1 - SPOT_ID: Rubisco) were contaminated prior to spotting with Bos taurus cDNA and thus might show hybridization. Other spots might be identified as empty of buffer. Keywords: cDNA, equine, articular cartilage, microarray

manufacturer: W.M. Keck Center, University of Illinois Urbana-Champaign

first: J

middle: N

last: MacLeod

first: M

middle: R

last: Band

first: Q

last: Sun

first: S

last: Coleman

manufacture_protocol: The cDNAs were spotted in 3x SSC buffer supplemented with 1.5 M betaine


title: SAGE:17:NlaIII:Brassica napus

status: Public on Jan 07 2009

last: GEO

organism: Brassica napus

technology: SAGE NlaIII

description: This is a virtual platform for SAGE libraries generated for Brassica napus using NlaIII as an anchor enzyme.

distribution: virtual


title: Yale Neurospora crassa 10K oligonucleotide v1.0

status: Public on Jan 10 2009

city: New Haven

first: Zhang

last: Zhang

state: CT

address: 165 Prospect Street, P.O.Box 208106

institute: Yale University

organism: Neurospora crassa

first: Jeffrey

last: Townsend

manufacture_protocol:
10,918 oligomers are on the chip.  10,526 oligos are based on MIPS or Broad Institute predicted ORFs.  8 oligos are Abmion ArrayControl Sense Oligo Spots (#1781).  The remaining 384 oligos (ChIP_Chip) were mainly designed in the intergenic regions.
40uM oligos in 3x SSC were printed onto Corning UltraGAPS slides.


title: SARST analysis of drinking water samples

city: paris

first: Jean Baptiste

last: Poitelon

address: 9 rue Schoelcher

country: France

institute: EaudeParis

organism: Bacteria

technology: SARST


title: ULaval/CRH_Tomato_200_v1.0

city: Québec

first: Jean-François

last: Dubuc

address: 2440 boul. Hochelaga

institute: Université Laval

organism: Solanum lycopersicum

description: The array consists of 8 sub-grids (2 meta-rows, 4 meta-columns) which are present in three replicates.

manufacturer: Institute of Nutraceuticals and Functional Foods

first: Andreja

last: Preradov

first: Dominique

last: Michaud

manufacture_protocol: This cDNA microarray from tomato was produce using spotted PCR fragment. The array was particularly enriched in genes associated with photosynthesis, carbon and nitrogen metabolism, transduction signals, stress and defense mechanisms and housekeeping functions. Primers for 120 genes were chosen using the software Primer3 (Rozen and Skaletsky, 2000). Genes or EST sequence were accessed via the National Center for Biotechnology Institute (NCBI). PCR product sizes were ranging from 200 to 800 bp. The PCR reactions were performed with the Expand high fidelity (Roche Diagnostics, Laval, QC, Canada) and PCR TGradient Thermocycler (Biometra, Goettingen, Germany). Each PCR product was purified with Montage PCR filter units (Millipore, Billerica, MA) and sequenced (SUCOF, QC, Canada) to confirm the authenticity of the amplified fragment. Amplification products of known genes were evaporated with Savant speedvac concentrator (Thermo Fisher Scientific, Waltham, MA), resuspended in 50% (v/v) dimethyl sulfoxide in double distilled water and arrayed with the VersArray ChipWriter Pro (Bio-Rad, Mississauga, ON, Canada) on GAPS II coated slides (Corning, Lowell, MA). All the genes present on the array were printed at least in six replicates in order to take into consideration of possible technical variations. The SpotReport Alien cDNA Array Validation System (Stratagene, La Jolla, CA), consisting of two artificial Alien sequences, was printed on the array. The print quality and the detection sensitivity of the arrays was access by the addition of these external and negative controls. A 700 pb fragment of the green fluorescent protein (GFP) was added to the microarray as a positive control.


title: USP Blastocladiella emersonii 4.6 K Blasto3773 v3

status: Public on Jan 05 2009

city: Goiania

first: Silvia Maria

last: Salem-Izacc

state: Goias

address: ICB, Campus 2

department: Bioquimica e Biologia Molecular

country: Brazil

institute: Universidade Federal de Goiás

organism: Blastocladiella emersonii

manufacturer: Universidade de São Paulo-Instituto de Química

first: Silvia

first: Raphaela

last: Georg

first: Suely

last: Gomes

manufacture_protocol: The array consists of cDNA inserts from 3,563 distinct B. emersonii expressed genes obtained through Georg and Gomes (2007) and Ribichich et al. (2005) works. These inserts were PCR amplified with the T7 forward and SP6 reverse primers. The amplified products were purified in Millipore Multiscreen Filtration Plates (MAFB NOB), eluted with Tris-HCl 10 mM, pH 8.0 to a final concentration of 100 to 200 ng/μl and spotted onto Star 7 slides (Amersham Biosciences) using a Generation III Microarray SpotterTM (Amersham Biosciences).


title: SAGE:10:NlaIII:Aedes aegypti

status: Public on Jan 12 2009

organism: Aedes aegypti

description: This is a virtual platform for SAGE libraries generated for Aedes aegypti using NlaIII as an anchor enzyme.


title: Nimblegen ENCODE HG18 385K

city: Cleveland

first: Mike

last: Schnetz

address: 10900 Eulid Ave

institute: Case Western Reserve University

first: Peter

last: Scacheri

manufacture_protocol: human genome tiling arrays covering ENCODE regions


title: Print_1115

status: Public on Jan 06 2009

city: Stanford

first: Anna

last: Brotcke

department: Microbiology and Immunology

institute: Stanford University

organism: Francisella tularensis

manufacture_protocol: Material is transferred from microtiter plate wells to the slide surface by an automated print head


title: Mouse lincRNA Expression Array (HD1 Platform)

status: Public on Jan 01 2009

city: Cambridge

first: Mitchell

last: Guttman

address: 7 Cambridge Center

institute: Broad Institute

organism: Mus musculus

description:
Platform annotation based on genome build mm8, NCBI Build 36.
The .ndf and .pos files are linked below as supplementary files.

first: John

last: Rinn

first: Eric

middle: S

last: Lander

manufacture_protocol: see manufacturer's web site www.nimblegen.com


title: Mouse lincRNA Multiple Array Design (HD1 Platform)

description:
Platform annotation based on genome build mm8, NCBI Build 36.
This array design was physically spread across 4 arrays.
The .ndf and .pos files are linked below as supplementary files.


title: Tortula ruralis cDNAs from Suppressive Subtractive Hybridization

city: Lubbock

first: Paxton

last: Payton

state: TX

address: 3810 4th Street

organism: Syntrichia ruralis

description: We established a cDNA microarray containing the inserts (PCR derived fragment) from each of the 768 individual SSH ESTs, with the exception of thirteen that failed to generate a PCR fragment. Twelve of the missing thirteen SSH EST cDNAs were replaced with PCR fragments from previously isolated T. ruralis cDNAs four of which, representing the ribosomal proteins S14, S16, L23 and L15 (Wood et al., 2000, Zeng and Wood 2000), were previously reported to be constitutively expressed and were added to serve as normalization genes. The remaining eight clones, Tr155, Tr217, Tr403, Tr416, Tr421 (described by Scott and Oliver, 1994), and TrCDPK (U82087) were added as either positive “up-regulated” (Tr155, Tr403, Tr421), negative “down-regulated (Tr217, Tr416), or neutral (TrCDPK) controls based on previous northern analyses. The cDNAs were printed from two 384 well plates in 12 blocks (two columns of 6) of 24 x 8 spots such that each SSH EST and controls were represented in triplicate. Each of the triplicate cDNAs was separated within the blocks to eliminate possible spatial hybridization bias. All hybridizations were duplicated as dye swaps with two separate RNA preparations, from large populations of individual gametophytes (isolated from a minimum of three separate clumps), serving as the source for the sscDNA Cy3 and Cy5 labeled targets.  The RNA preparations for the Total polyA RNA were by necessity separate samples from those used to isolate Polysomal poly A RNA.

manufacturer: USDA-ARS Cropping Systems Research Laboratory, Lubbock, Texas, USA

first: Melvin

last: Oliver

manufacture_protocol:
T. ruralis ([Hedw.] Gaertn, Meyer and Scherb) gametophytes were collected and stored dry at room temperature. For experiments, gametophyte tissue was hydrated for 48h as described previously (Oliver, 1991; Scott and Oliver, 1994) to ensure recovery of the tissue to full activity from its exposure to drying environments in the field. These gametophytes were sampled and flash frozen to represent hydrated controls. Rapid-dried moss was prepared by placing fresh gametophyte on filter paper over activated silica gel in a petri dish as described previously (Bewley and Thorpe, 1974).  The rehydrated state was obtained by immediately flash freezing in liquid nitrogen following 2h of rehydration.  Slow-dried moss was prepared by placing fresh gametophytes of known weights on a nylon mesh in a closed chamber over a stirred saturated solution of sodium nitrite at 20˚C (RH 66%) for 24 h. Using this regime the air-dried weight (ca. 20% of original fresh weight) was obtained within 6h (Oliver, 1991).
Two subtractive suppression hybridization libraries were constructed using a PCR-Select cDNA Subtraction Kit  (Clontech, Palo Alto, California, USA) following manufacturer’s instructions: Slow-dried Polysomal (SDP) and Rapid-dried Rehydrated polysomal (RDPR).  For each cDNA subtraction, hydrated control gametophytic poly (A+) RNA was used as the source for driver cDNA.  2μg of mRNA was the starting material for both tester and driver. The subtracted cDNAs were cloned into the T/A vector pUCR19r (Oliver et al., 2001) and transformed into DH5α cells (Invitrogen, Carlsbad, CA). Transformants were selected on LAXI plates (100μg/ml AMP, 0.008% X-Gal, 200μM IPTG) for blue/white screening.  White colonies were picked into 96-well plates containing 200μl of LB/AMP (100μg/ml) and allowed to grow overnight shaking at 37˚C.  80μl of LB:glycerol (1:1) was added to the wells (final concentration of 17% glycerol), and stored at –80˚C.
PCR generated DNA fragments in 2.5X SSC, 0.1% sarcosyl at a concentration of 250ng/ul were arrayed in 384-well plates for printing.  Poly-L-lysine coated slides were printed using a GMS 417 microarrayer (Affymetrix, Santa Clara, CA, USA).  Three replicates for each fragment (2 hits/spot) were printed for each block, with two blocks per slide.  The slides were processed and the cDNAs cross-linked as described by Hegde et al., 2000 and stored in the dark at room temperature.


title: Escherichia coli K-12 MG1655 tiling array (Nimblegen Design)

status: Public on Jan 03 2009

city: Madison

first: Rachel

middle: Anne

last: Mooney

state: WI

address: 1550 Linden Drive

department: Biochemistry

institute: University of Wisconsin-Madison

first: Sarah

middle: E

last: Davis

first: Jason

last: Peters

first: Jennifer

last: Rowland

first: Aseem

middle: Z

last: Ansari

first: Robert

last: Landick

manufacture_protocol: Nimblegen maskless oligonucleotide synthesized array that tiles the E. coli K-12 MG1655 genome with Tm matched probes at a length of 45bp or greater with ~24bp spacing.


title: 3D-Gene Human miRNA Oligo chip v10.1

city: Kamakura

first: Satoko

last: Takizawa

state: Kanagawa

address: 6-10-1 Tebiro

department: New Frontiers Research Laboratories

institute: TORAY Industries, Inc.

manufacture_protocol: See manufacturer's web site


title: Umass/Reppert Danaus plexippus EST custom Nimblegen array

city: Worcester

first: Steven

last: Reppert

state: MA

address: 364 Plantation St

department: Neurobiology

institute: University of Massachusetts Medical School

organism: Danaus plexippus

description: All information related to EST and contigs are located at the ESTIMA website (see web link).

first: Haisun

last: Zhu

last: Gegear

first: Amy

last: Casselman

first: Sriramana

last: Kanginakudru

manufacture_protocol: Roche Nimblegen Custom 4-plex gene expression array


title: PE aCGH Human 2.6K v1

status: Public on Dec 25 2008

city: Hamilton

first: Mickie

last: Bhatia

state: Ontario

address: 1200 Main Street West

institute: McMaster Stem Cell and Cancer Research Institute (SCC-RI)

description: SpectralChip 2,600 array

first: Tamra

last: Werbowetski-Olgilvie

first: Marc

last: Bosse

first: Morag

last: Stewart

first: Angelique

last: Schnerch

first: Veronica

last: Ramos-Mejia

first: Anne

last: Rouleau

first: Tracy

last: Wynder

first: Mary-Jo

last: Smith

first: Steve

last: Dingwall

first: Tim

last: Carter

first: Christopher

last: Williams

first: Charles

last: Harris

first: Joanna

last: Dolling

first: Doug

last: Boreham

manufacture_protocol: See manufacturer's website.


title: Mouse lincRNA Tiling Array (HD2 Platform)

first: Mitchell

last: Guttman

description:
50mer oligo probes
Platform annotation based on genome build mm8, NCBI Build 36
The .ndf and .pos files are linked below as supplementary files

first: John

last: Rinn

first: Eric

last: Lander


title: ACGT Pearl Millet SSH library 4K v1.0

city: Pretoria

first: Bridget

middle: Genevieve

last: Crampton

address: PO Box 395

country: South Africa

institute: CSIR Biosciences

organism: Cenchrus americanus

manufacturer: ACGT Microarray Facility, University of Pretoria, Pretoria, South Africa (http://www.microarray.up.ac.za )

first: Danie

last: Theron

manufacture_protocol:
1. PCR fragments of a cloned cDNA suppression subtractive hybridisation (SSH) library are arrayed in Genetix X7022 384-well plates.
2. Each well contains a final concentration of 125 ng/µl PCR product in 50% DMSO spotting solution (v/v).
3. Between printing runs the plates are stored at -20°C.
4. Prior to printing the plates are incubated at room temperature for 30 min to aid temperature equilibration.
5. Plates are centrifuged at 2000 x g for 5 minutes prior to printing.
6. Printing is performed on the Molecular Dynamics Generation III spotter.
7. Seals are removed and plates placed in the spotter immediately prior to initiating the printing process to prevent unnecessary evaporation.
8. A relative humidity of 50% is maintained in the spotter cabinet during printing runs.
9. Following completion of the printing run the plates are sealed with a new Abgene adhesive plate seal and stored at -20°C.


title: Mouse lincRNA Tiling Array (HD1 Platform)


title: Mouse_Oligo_Ia_BMFZ

city: Duesseldorf

first: Antje

last: Lindecke

address: Universitaetsstr. 1

department: BMFZ

institute: Universitaet Duesseldorf

first: Karl

last: Köhrer

first: Jürgen

last: Mehrens

manufacture_protocol:
Spotter: QArray2 (Genetix)
Pins: split pins (Genetix, #K2805)
Spotting Buffer: aminospotting buffer Genetix, #K2050)
Slide: UltraGaps (Corning)
Oligos: Mus musculus 70mer Oligo-Set, Plates 1 to 48 (Operon)


title: SABiosciences human 440 Cancer Oligo GEArray v1

first: Mickie

last: Bhatia

description: The Oligo GEArray® Human Cancer Microarray profiles profiles the expression of 440 genes that include members of several different pathways frequently altered during the progression of cancer. This array allows you to examine specific aspects of tumor progression. The genes represented by this array include but are not limited to tumor suppressors, oncogenes, signal transduction molecules, growth factors, growth factor receptors, and angiogenesis factors. Through a simple side-by-side hybridization experiment you can determine differential gene expression between your samples.

first: Tamra

last: Werbowetski-Olgilvie

first: Marc

last: Bosse

first: Morag

last: Stewart

first: Angelique

last: Schnerch

first: Veronica

last: Ramos-Mejia

first: Anne

last: Rouleau

manufacture_protocol: See manufacturer's website


title: Valencia yeast v5r2

city: Burjassot

first: Jose

middle: E.

last: Perez-Ortin

state: Valencia

address: Dr. Moliner 50

department: Bioquimica y Biologia Molecular

country: Spain

institute: Universitat de Valencia

description: Nylon macroarray (7 x 11 cm) with 6019 single spotted yeast ORF probes. Probes are PCR fragments comprising the whole ORF (5568 cases) or a 1000 bp-fragment from the ORF 3' end (451 cases).

manufacturer: Seccion de Chips de DNA-SCSIE (University of Valencia)

last: Garcia-Martinez

manufacture_protocol: See Alberola et al. (2004), International Microbiology 7:199-206


title: UT/TH_all-8mer-v1

status: Public on Jun 20 2008

city: Toronto

first: Lourdes

last: Pena-Castillo

address: 160 College St. Room 1350

department: Banting and Best Department of Medical Research

institute: University of Toronto

organism: synthetic construct

description: This protein binding microarray (PBM) involved binding GST-tagged proteins to custom-designed, double-stranded 44K Agilent microarrays in order to determine their sequence preferences. The method is described in Berger et al., Nature Biotechnology 2006.  A key feature is that the microarrays are composed of de Bruijn sequences that contain each 10-base sequence once and only once, providing an evenly balanced sequence distribution. Individual de Bruijn sequences have different properties, including representation of gapped patterns.  The original Berger et al. array sequences  are available via a EULA at http://the_brain.bwh.harvard.edu/UPBMseqn/UPBMseqn_agreement.html.

manufacture_protocol: In situ oligosynthesized array with proprietary synthetic sequences that target all possible 8-base sequences. The sequences shown in the below table are not the actual sequences that are printed on the array.


title: Brown/Botstein lab, Stanford University Saccharomyces cerevisiae yKA1 array

status: Public on Dec 14 2007

city: Spokane

first: Kirk

last: Anders

state: WA

address: 502 E. Boone Ave, AD 5

department: Biology

institute: Gonzaga University

description:
Tip Configuration: Standard 48-tip
Columns per Sector: 16
Column Spacing: 270
Row Spacing: 270

manufacture_protocol:
Manufactured as per DeRisi et al., Science278:680, 1997.
DNA: PCR amplifications using yeast ORF primer set from Research Genetics, Spring 2002, resuspended in 3x SSC.
Slides: poly-lysine coated.
Custom printer in room CCSR 260, Stanford School of Medicine.
Printed May 2003.


title: UNC_Lieb_Agilent_Celegans_185K

status: Public on Jan 31 2009

city: Chapel Hill

first: Christina

middle: M

last: Whittle

address: 406 Fordham Hall, CB#3280

institute: University of North Carolina-Chapel Hill

organism: Caenorhabditis elegans

description: Agilent genome tiling array covering the entire genome in 185K features at an average 600bp start-to-start resolution.

first: Elena

last: Elena Lazakovitch

first: Richard

last: Gronostajski

first: Jason

middle: D

last: Lieb

manufacture_protocol: see manufacturer's web site at http://www.agilent.com/