title: A Xenopus DNA microarray approach to identify novel direct BMP target genes involved in early embryonic development

status: Public on Mar 05 2005

city: Irvine

first: Yongchol

last: Shin

address: 4115 McGaugh Hall

department: Developmental and Cell Biology

institute: University of California, Irvine

summary:
Bone Morphogenetic Proteins (BMPs), a subgroup of the TGF-beta superfamily, were originally isolated from bone on the basis of their ability to induce ectopic bone development.  While BMPs are involved in a wide range of developmental and physiological functions, very few vertebrate target genes in this pathway have been identified.  To identify target genes regulated by the BMP growth factor family in Xenopus, large-scale microarray analyses were conducted to discover genes directly activated by this factor in dissociated animal cap tissues treated with a combination of the protein synthesis inhibitor cycloheximide and BMP2.
Keywords = Xenopus
Keywords = BMP
Keywords = microarray
Keywords = development
Keywords = target genes
Keywords: parallel sample

first: Daniel

last: Peiffer

first: Andreas

last: Von Bubnoff

first: Atsushi

last: Kitayama

first: Makoto

last: Mochii

first: Naoto

last: Ueno

first: Ken

middle: W

last: Cho


title: Human embryonic stem cells

status: Public on Jan 19 2004

city: Singapore

first: Mark

last: Richards

country: Singapore

institute: National University of Singapore

summary:
Undifferentiated human embryonic stem cells grown on mouse embryonic fibroblast feeder layers. HES3 and HES4 are proprietary human ES cell lines of ES Cell International Pte Ltd. Singapore.
Keywords: other

first: Woon-Khiong

last: Chan


title: SAGE Hiroshima gastric cancer tissue

status: Public on Jul 21 2003

city: Hiroshima

first: Wataru

last: Yasui

department: Molecular Pathology

country: Japan

institute: Hiroshima University Graduate School of Biomedical Sciences

summary:
Human primary gastric cancer tissue SAGE libraries. Profile of the genes expressed in well and poorly differentiated gastric cancer, early and advanced gastric cancer, scirrhous type gastric cancer, and　lymph node metastasis determined through SAGE.
Keywords = gastric cancer, histology, early gastric cancer, advanced gastric cancer, lymph node metastasis, scirrhous type gastric cancer
Keywords: other

first: Naohide

last: Oue


title: Effect of TERT and ATM on Gene Expression Profiles in Human Fibroblasts

status: Public on Jan 17 2004

city: Vancouver

first: Agnes

last: Baross

state: BC

address: 600 West 10th Avenue

country: Canada

institute: British Columbia Cancer Agency

summary:
Gene expression profiling was performed by use of serial analysis of gene expression (SAGE) on BJ normal human skin fibroblasts, A-T cells, and BJ and A-T cells transduced with hTERT cDNA and expressing telomerase activity.
Keywords = telomere
Keywords = telomerase
Keywords = TERT
Keywords = ataxia telangiectasia mutated
Keywords = ATM
Keywords = serial analysis of gene expression
Keywords = SAGE
Keywords = fibroblast
Keywords: parallel sample

first: Mike

last: Schertzer

first: Scott

middle: D

last: Zuyderduyn

first: Steven

middle: J

last: Jones

first: Marco

middle: A

last: Marra

first: Peter

middle: M

last: Lansdorp


title: AIDS-related Kaposi's sarcoma

status: Public on Jul 16 2003

city: Amsterdam

first: Antoinette

last: van der Kuyl

department: Human Retrovirology

country: Netherlands

institute: Academic Medical Centre, University of Amsterdam

summary:
Kaposi's Sarcoma (KS) is a proliferation of aberrant vascular structures lined by spindle cells, and is caused by a gammaherpes virus (HHV8/KSHV). Its course is aggravated by co-infection with HIV-1, where the timing of infection with HIV-1 and HHV8 is important for the clinical outcome. METHODS: In order to better understand the pathogenesis of KS, we have analysed tissue from two AIDS-KS lesions, and from normal skin by serial analysis of gene expression (SAGE). Semi-quantitative RT-PCR was then used to validate the results. RESULTS: The expression profile of AIDS-related KS (AIDS-KS) reflects an active process in the skin. Transcripts of HHV8 were found to be very low, and HIV-1 mRNA was not detected by SAGE, although it could be found using RT-PCR. Comparing the expression profile of AIDS-KS tissue with publicly available SAGE libraries suggested that AIDS-KS mRNA levels are most similar to those in an artificially mixed library of endothelial cells and leukocytes, in line with the description of KS lesions as containing spindle cells with endothelial characteristics, and an inflammatory infiltrate. At least 64 transcripts were found to be significantly elevated, and 28 were statistically downregulated in AIDS-KS compared to normal skin. Five of the upregulated mRNAs, including Tie 1 and sialoadhesin/CD169, were confirmed by semi-quantitative PCR to be elevated in additional AIDS-KS biopsies. Antibodies to sialoadhesin/CD169, a known marker of activated macrophages, were shown to specifically label tumour macrophages. CONCLUSION: The expression profile of AIDS-KS showed 64 genes to be significantly upregulated, and 28 genes downregulated, compared with normal skin. One of the genes with increased expression was sialoadhesin (CD169). Antibodies to sialoadhesin/CD169 specifically labelled tumour-associated macrophages, suggesting that macrophages present in AIDS-KS lesions belong to a subset of human CD169+ macrophages.
Keywords: other

first: Marion

last: Cornelissen

middle: C

first: Remco

last: van den Burg

first: Audrey

last: van der Schoot

first: John

middle: T

last: Dekker

first: Jolanda

last: Maas

first: Fokla

last: Zorgdrager

first: Bert

last: Tigges

first: Carel

last: van Noesel

first: Jaap

last: Goudsmit

first: Celeste

last: Lebbé