status: Public on Aug 27 2003

first: Erin

last: Osborne

address: 260 Panama Dr.

department: Department of Plant Biology

institute: Carnegie Institute of Washington

summary:
Analysis of gene expression in Arabidopsis thaliana leaf, stem and flower tissues.

Columbia (Col-0) Arabidopsis thaliana plants were grown at a density of 4 plants per 5 inch square pot either in a growth chamber or a green house set to 25*C by day, 20*C by night. Days were set to a 16hr photoperiod with 125 micro mol m-2s-1 fluorescent irradiation.
Expanding leaves were harvested 15 days post germination in the middle of the photoperiod (3 replicates).
Expanding upper 2" of the stem with siliques and pedicels removed were harvested 29 days post germination in the middle of the photoperiod (4 replicates).
Developed flowers and unopened buds were harvested 29 days post germination in the middle of the photoperiod (4 replicates).

RNA and Microarray Methods: Total RNA was extracted from the plants using the Trizol method (Invitrogen, Ramonell et al. (2002) Mol. Plant Pathol. 3: 301) and purified with a silica membrane column (Qiagen, RNeasy). Twenty micrograms biotinylated complementary RNA (cRNA) was prepared as described (Hernan et al. (2003) Cancer Res. 63, 140) from the purified total RNA. The resulting cRNA was used to hybridize ATH1 Arabidopsis GeneChips (Affymetrix) using the manufacturer's protocols. The array images were analyzed with the Affymetrix Microarray Suite 5.0 software with the target intensity set to 500. Normalized signal intensities were generated by multiplying signal intensities by ratio figure which set GapC (ID_REF = 258588_s_at) to 8500.
Keywords: other


title: Flower development

status: Public on Aug 06 2003

city: Tübingen

first: Markus

last: Schmid

address: Speemannstrasse 37-39

department: Molecular Biology

country: Germany

institute: Max Planck Institute for Developmental Biology

summary:
Wild type and mutanat Arabiposis plants grown in short days (9L:15D) for 30 days at 21°C, then shifted to long days (16L:8D).

Genotypes:
Columbia wild type (Col-0)
Landsberg erecta (Ler)
leafy-12 (lfy-12, in Col-0)
constans-2 (co-2, in Ler)
flowering locus T-2 (ft-2, in Ler)

Time points:
0, 3, 5, and 7 days after shift to long days
Keywords = flowering
Keywords: time-course

first: Detlef

last: Weigel

first: Jan

middle: U

last: Lohmann


title: Arabidopsis shoot apices dissection

city: Raleigh

first: Jose

middle: M

last: Alonso

state: NC

department: Genetics

country: USA

institute: North Carolina State University

summary:
Comparisin of short day and long day growth conditions for wild-type Arabidopsis seeds (ecotype Col-0).
Keywords: other


title: Overexpression of miR156b and miR164b (floral apex)

status: Public on Feb 01 2005

city: Tuebingen

first: Javier

middle: Fernando

last: Palatnik

address: 37-39 Spemannstrasse

institute: Max Planck Institute

summary:
RNA was harvested from floral apices of long-day grown plants. Overexpression of miR156b and 164b versus control.
Keywords: parallel sample

first: Rebecca

last: Schwab

middle: F

first: Carla

last: Schommer


title: Seedling ethylene treatment

summary:
Effect of ethylene treatment on wild-type Arabidopsis seedlings (ecotype Col-0).
Keywords: other

first: Plinio

last: Guzman

first: Joseph

middle: R

last: Ecker


title: Activation-tagged jaw-d mutant plants

status: Public on Jul 09 2003

summary:
Analysis of gene expression in activation-tagged jaw-d mutant plants. Total RNA was extracted from the aerial parts of two weeks old jaw-d  and control plants.
Keywords: other

first: J

first: D