title: AGEMAP_Liver

first: Supriyo

last: De

first: Jacob

last: Zahn

first: Poosala

last: Suresh

first: Art

last: Owen

first: Ana

last: Lustig

first: Arnell

last: Carter

first: Ashani

last: Weeraratna

first: Dennis

last: Taub

first: Myriam

last: Gorospe

first: Krystyna

last: Mazan-Mamczarz

last: Boheler

first: Xiangru

last: Xu

first: Mark

last: Mattson

first: Geppino

last: Falco

first: Minoru

last: Ko

last: Schlessinger

first: Jeffery

last: Firman

first: Sarah

last: Kummerfeld

first: William

last: Wood

first: Alan

last: Zonderman

first: Stuart

last: Kim

first: Kevin

last: Becker


title: AGEMAP_Eye

status: Public on Dec 28 2007

city: Baltimore

first: Supriyo

last: De

state: Maryland

address: 251 Bayview Blvd

department: Laboratory of Genetics and Genomics

country: USA

institute: NIA-IRP, NIH

summary:
To assess the molecular changes associated with aging in different tissues of mice using cDNA microarrays to examine the transcriptomes of young, middle aged and old mice.
Mice of both genders were examined in relation to the effects of age in multiple tissues.
Keywords: age- and gender comparison.

overall_design: Up to 40 mice were compared according to age and gender.

first: Jacob

last: Zahn

first: Poosala

last: Suresh

first: Art

last: Owen

first: Donald

last: Ingram

last: Lustig

last: Carter

first: Edward

last: Lakatta

first: Kenneth

first: David


title: Age Map

status: Public on Nov 24 2004

summary:
Analysis of the effect of aging and caloric restriction on liver tissue
Keywords: other


title: Age Map, Cerebellum (B)

summary:
To assess the transcriptional changes in different brain regions and spinal cord associated with aging and either a caloric restricted (CR) or ad libitum (AL) diet. cDNA microarray and quantitative PCR analyses were used to examine the transcriptomes of various neuronal tissues in young, middle aged and old mice. Mice of both genders were examined as well as the effects of their placement on either caloric restricted (CR) or ad libitum (AL) diets.
Keywords: age-, gender-, and diet- comparison

overall_design: 67 mice were compared according to age, gender, and diet

first: Xiangru

last: Xu

first: Ming

last: Zhan

first: Wenzhen

last: Duan

first: Vinayakumar

last: Prabhu

first: Randall

last: Brenneman

last: Wood III

first: Jeff

first: Huai

last: Li

first: Peisu

last: Zhang

first: Carol

last: Ibe

middle: B

first: Dan

middle: L

last: Longo

first: Suresh

last: Poosala

middle: G

first: Mark

middle: P

last: Mattson


title: NIA/NIH AGEMAP Brain Regions

first: Supriyo

last: De

summary: This SuperSeries is composed of the SubSeries listed below.

overall_design: Refer to individual Series

first: William

last: Firman

first: Alan

last: Zonderman

first: Kevin

last: Becker


title: Age Map, Hippocampus (A)


title: TRF2 Inhibition-Mediated Degradation Derepresses the Neuronal Differentiation Program

status: Public on Jul 01 2007

summary:
Telomere binding factor 2 (TRF2), is a protein that plays a major role in the maintenance of telomere integrity.  In mitotic normal and transformed cells, TRF2 inhibition triggers a rapid telomere DNA damage response that results in cell senescence or apoptosis.  Here we provide evidence that TRF2 plays a role suppressing neuronal differentiation.  TRF2 interacts with the RE1-silencing transcription factor (REST) in nuclear PML protein-containing compartments of neuronal cells in vivo.  Inhibition of TRF2 function with a dominant-negative form of TRF2 elicits a telomeric DNA damage response, and disrupts the TRF2-REST complex resulting in proteasomal degradation of REST.  Overexpression of REST impairs the ability of DN-TRF2 to induce neuronal differentiation, indicating that enhanced degradation of REST is sufficient to account for the differentiation-inducing effect of DN-TRF2.  REST degradation derepresses RE1-regulated genes (L1CAM, BDNF, b3-tubulin, syntaxin and others) resulting in morphological and functional differentiation of neurons.  Our findings identify a novel interaction between the telomeric protein TRF2 and REST which regulates the molecular differentiation program of neurons.
Keywords: transfection and molecular inhibition

overall_design: Cells were transfected with GFP vector, WT-TRF2 or DN-TRF2,  and were maintained under G418 selection for 7 days.  To confirm an inhibitory effect of DN-TRF2 on cell proliferation cells were counted daily during a 4 day post-transfection period.  Gene array analysis was performed using NIA MGC1 9600 human gene cDNA microarray on 6 samples; 3 controls and 3 DN-TRF2 positive.

first: Michael

middle: J

last: Pazin

first: Caroline

middle: M

last: Dilley


title: Age Map, Cerebral Cortex (A)

first: Xiangru

last: Xu

first: Ming

last: Zhan


title: Age Map, Thymus

status: Public on May 18 2007

summary:
To assess the molecular changes associated with thymic loss, cDNA microarray and quantitative PCR analyses were used to examine the transcriptomes of the thymi of young, middle aged and old mice.
Mice of both genders were examined as well as the effects of their placement on either caloric restricted (CR) or ad libitum (AL) diets.
Keywords: age-, gender-, and diet- comparison

first: Ana

last: Lustig

first: Ashani

middle: T

last: Weeraratna

last: Wood

first: Diane

last: Teichberg

first: Dorothy

last: Bertak

first: Arnell

last: Carter

first: Jeffrey

first: Dennis

middle: D

last: Taub


title: NIA/NIH AGEMAP Multiple Tissues

first: Jacob

last: Zahn

first: Poosala

last: Suresh

first: Art

last: Owen

first: Donald

last: Ingram

first: Myriam

last: Gorospe

first: Krystyna

last: Mazan-Mamczarz

first: Edward

last: Lakatta

first: Kenneth

last: Boheler

first: Geppino

last: Falco

first: Minoru

last: Ko

first: David

last: Schlessinger

first: Jeffery

first: Sarah

last: Kummerfeld

first: Stuart

last: Kim