title: Transcriptomic signature induced in bone marrow Mesenchymal Stem/Stromal Cells (MSC) after interaction with malignant multiple myeloma cells: implications in myeloma progression.

status: Public on Nov 23 2014

city: Salamanca

first: Javier

last: De Las Rivas

address: Campus Miguel de Unamuno

department: CSIC and University of Salamanca

country: Spain

institute: Cancer Research Center (CiC-IBMCC)

summary: Mesenchymal stem/stromal cells (MSCs) derived from the BM of healthy donors (dMSCs) and myeloma patients (pMSCs) were co-cultured with the model myeloma cell line - MM.1S -, and the gene expression profile of MSCs induced by this interaction was analyzed using high density oligonucleotide microarrays. Deregulated genes in co-culture common to both d/pMSCs revealed functional involvement in tumor microenvironment cross-talk, myeloma growth induction and drug resistance, angiogenesis and signals for osteoclast activation and inhibition of osteoblasts. Additional genes induced by co-culture were exclusively deregulated in pMSCs and were predominantly associated to RNA processing, the ubiquitine-proteasome pathway, regulation of cell cycle and Wnt signaling.

overall_design: Primary MSCs from bone marrow (BM) samples of healthy donors (n=8) and multiple myeloma (MM) patients (n=13) were generated as described by Garayoa et al. 2009 (PMID:19357701). Ficoll-Paque density gradient separation medium was used to isolate mononuclear cells from BM aspirates. MSCs were selected by adherence to plastic and subsequently expanded until passage 2 when they were used for co-culture. For the MSC-MM.1S co-cultures we used a 6-well format transwell system with 1 µm pore size membrane. Briefly, 2 X 10^4 MSCs were first cultured attached to the lower side of the membrane, and when reached ≈ 85% confluence 1 X 10^6 MM.1S cells were seeded on the upper side. After 24 h co-culture in RPMI 1640 medium supplemented with 10% FBS and antibiotics, MSCs were recovered by trypsinization, washed once in PBS and stored at -80°C in RLT buffer (Qiagen) until RNA extraction. Absence of MM.1S cells in the recovered MSC population was assessed in selected samples by flow cytometry analysis.

first: Antonio

last: Garcia-Gomez

first: E

last: Ocio

last: Diaz-Rodriguez

first: J

middle: C

last: Montero

first: M

last: Martin

last: Blanco

first: F

last: Sanchez-Guijo

first: A

last: Pandiella

last: San Miguel

first: Mercedes

last: Garayoa


title: Microarray expression data from three arterial tortuosity syndrome (ATS) patients' skin fibroblasts with recessive SLC2A10 mutations

status: Public on Sep 21 2015

city: Brescia

first: Nicola

last: Chiarelli

state: Lombardy

address: Viale Europa 11

department: Molecular and Translational Medicine

country: Italy

institute: University of Brescia

summary:
To screen for candidate genes that may contribute to the pathogenesis of ATS
Transcriptome-wide expression profiling using the Affymetrix Gene 1.0 ST platform comparing the gene expression patterns of skin fibroblasts of three ATS patients with those of three healthy individuals

overall_design: Comparison between three ATS human fibroblasts and three healthy individuals


title: Identifying active cis-regulatory elements during the development of the limbs and external genitalia in the mouse and lizard.

status: Public on Oct 01 2015

city: Athens

first: Carlos

middle: Rafael

last: Infante

state: GA

address: 500 D.W. Brooks Dr

department: Genetics

country: USA

institute: University of Georgia

type: Genome binding/occupancy profiling by high throughput sequencing

summary: The morphologies of the amniote phallus and limbs differ dramatically, but these structures share signaling pathways and patterns of gene expression in early development. Thus far, the extent to which genital and limb transcriptional networks share cis-regulatory elements has remained unexplored. Using chromatin immunoprecipitation with an antibody against the histone modification mark H3K27ac followed by Illumina high-throughput sequencing, we identify thousands of active enhancers in developing limbs, genital tubercle, and additional embryonic tissues of the mouse and green anole lizard (Anolis carolinensis). We show in global analyses of cis-regulatory activity that embryonic limbs and genitalia display overlapping patterns of enhancer activity, and that many H3K27ac-marked regions are shared between mouse and green anole. Our findings support the hypothesis that the amniote phallus evolved through co-option of a preexisting appendage developmental program.

sample_organism:
Mus musculus
Anolis carolinensis

platform_organism:
Mus musculus
Anolis carolinensis

overall_design: ChIP-Seq was performed using an antibody against the histone mark H3K27ac on mouse E11.5 forelimb (2 replicates), E11.5 hindlimb (2 replicates), E12.5 genital tubercle (2 replicates), E11.5 flank mesenchyme (single sample), and E10.5 eye (2 replicates). For the green anole lizard, H3K27ac ChIP-Seq was performed on single replicates of pooled stage TS7/8 forelimbs, TS7/8 hindlimbs, or TS9 to TS11 hemiphallus. For each tissue type, a sample of sheared chromatin prior to antibody incubation and pulldown (input DNA) was saved for the generation of control libraries to detect enrichment in the ChIP samples.

middle: R

first: Alexandra

middle: G

last: Mihala

first: Sungdae

last: Park

first: James

middle: D

last: Lauderdale

first: Douglas

middle: B

last: Menke


title: Gene expression profiling of human Ewing sarcoma cells after knockdown of EGR2 or EWSR1-FLI1.

status: Public on Jul 26 2015

city: Heidelberg

first: Thomas

middle: Georg Philipp

last: Grunewald

address: Im Neuenheimer Feld 280

department: Division of Translational Pediatric Sarcoma Research

country: Germany

institute: German Cancer Research Center (DKFZ)

summary: To get insight in the functional role of EGR2 for Ewing sarcoma, we performed a transcriptional profiling of Ewing sarcoma cells after knockdown of EGR2 and compared the resulting transcriptional signature with that of EWSR1-FLI1-silenced Ewing sarcoma cells. In accordance with the strong EGR2-induction by EWSR1-FLI1, both genes highly significantly overlap in their transcriptional signatures. Gene-set enrichment analyses (GSEA) and DAVID (Database for Annotation, Visualisation and Integrated Discovery) gene ontology analyses indicated a strong impact of EGR2 on cholesterol and lipid biosynthesis resembling its function in orchestrating lipid metabolism of myelinating Schwann cells.

overall_design: A673 and SK-N-MC Ewing sarcoma cells were transfected with specific siRNAs directed against EGR2 or EWSR1-FLI1 or non-targeting control siRNA. 48 h thereafter RNA was harvested and processed for microarray analysis.

first: Olivier

last: Delattre


title: Transcriptomic changes in Drosophila tissues under Time-restricted Feeding (TRF)

status: Public on Feb 18 2015

city: La Jolla

first: Christopher

last: Benner

state: California

address: 9500 Gilman Dr. MC 0640

department: Medicine

institute: University of California, San Diego (UCSD)

type: Expression profiling by high throughput sequencing

summary: Next generation sequencing was used to assess the changes along a diurnal cycle in the transcriptome of Drosophila head, periphery (entire body except the head) or the heart at 3, 5 or 7 weeks of age under ad libitum feeding (ALF) or time-restricted feeding (TRF).

first: Shubhroz

last: Gill

first: Girish

last: Melkani

first: Satchidananda

last: Panda


title: The miR-146b-3p/PAX8/NIS Regulatory Circuit Modulates the Differentiation Phenotype and Function of Thyroid Cells During Carcinogenesis

status: Public on Aug 24 2015

first: Javier

last: Perales-Paton

state: Baden-Württemberg

address: Im Neuenheimer Feld 267

department: Faculty of Medicine

institute: Heidelberg University

type: Non-coding RNA profiling by high throughput sequencing

summary: To comprehensively characterize microRNAs (miRNA) expression and their target genes in thyroid cancer, we performed next-generation sequencing expression analysis of this disease. Recent studies have found that only the most abundant microRNAs mediate significant target suppression. We sequenced small RNA from 8 papillary thyroid carcinomas (PTC) with paired samples of normal thyroid tissue. We found that only a small set of abundant miRNAs are differentially expressed after pair-wise comparison (12 upregulated and 8 downregulated) reaching the minimum threshold amount to repress target mRNAs. We integrated computational prediction of potential targets and mRNA sequencing from the paired normal and tumor thyroid tissues from the same eight patients with PTC. The integrated analyses identified a master microRNA regulatory network in PTC that is involved in essential biological processes such as thyroid differentiation. As both mature products of miR-146b (miR-146b-5p and -3p) were among the most abundant upregulated in tumors, we unveil their target genes and found that miR-146b-3p specifically binds to the 3`UTR of PAX8 and NIS, leading to an impaired translation of the proteins and subsequently decreasing the iodide uptake of the cells.  Furthermore, we show that mir-146b and PAX8 regulate each other, describing a novel regulatory circuit that determines the differentiated phenotype of PTC. In conclusion, our integrative genomic analysis uncovers the target genes of two of the most upregulated miRNAs and highlights the importance of a miR-146b3p-PAX8-NIS regulatory circuit that determines thyroid differentiation in thyroid cancer.

overall_design: Samples from Papillary Thyroid Carcinoma tumors (n=8) and contralateral normal thyroid tissue from the same patient (n=8) were collected at the Biobank of the Hospital Universitario La Paz (Madrid, Spain). The clinical characteristics of patients are summarized in Table S1. Surgically removed tissues were quickly frozen in liquid nitrogen until analysis. The samples were snap frozen on dry ice and stored at -80°C.

first: Garcilaso

last: Riesco-Eizaguirre

first: Leon

last: Wert-Lamas

last: Perales-Patón

first: Ana

last: Sastre-Perona

first: Lara

middle: P

last: Fernández

first: Pilar

last: Santisteban


title: Human CD8 T central memory (Tcm) cells: Control vs. norepinephrine (NE) treated

status: Public on Mar 01 2015

city: Baltimore

first: Nan-Ping

last: Weng

address: 251 Bayview Blvd

department: LMBI

institute: NIA

summary: Transcriptional profiling of CD8 Tcm cells comparing untreated control cells to 16 hour norepinephrine treated cells. The goal was to examine the impact of norepinephrine treatment on CD8 Tcm cell global gene expression compared to untreated cells, and also to examine changes that occurred in resting cells and activated cells 24 and 72 hours after stimulation with CD3/28 beads.

overall_design: Two-conditional experiment: Control vs. NE treated CD8 Tcm cells. Biological replicates: 3 replicates for 0 hour and 24 hours, and 2 replicates for 72 hours. Each sample consisted of pooled RNA from 3 different donors.

first: Christina

last: Slota

first: Guobing

last: Chen

first: Alvin

last: Shi


title: Genetic background of immune complications

status: Public on Jun 02 2015

city: Krakow

first: Szymon

last: Skoczen

address: Wielicka 265

department: Clinical Immunology

country: Poland

institute: Chair of Clinical Immunology and Transplantation, Jagiellonian University Medical College

summary:
Differencies between groups between pre and post haematopoietic stem cell transplantation children
Immune reactions are among the most serious complications observed after hematopoietic stem cell transplantation (HSCT) in children. Microarray technique allows for simultaneous assessment of expression of nearly all human genes. The objective of the study was to compare the whole genome expression in children before and after HSCT. A total of 33 children referred for HSCT were enrolled in the study. In 70% of the patients HSCT was performed for the treatment of neoplasms. Blood samples were obtained before HSCT and six months after the procedure. Subsequently, the whole genome expression was assessed in leukocytes using GeneChip® HumanGene 1.0 ST microarray. The analysis of genomic profiles before and after HSCT revealed altered expression of 124 genes. Pathway enrichment analysis revealed upregulation of five pathways after HSCT: allograft rejection, graft-versus-host disease, type I diabetes mellitus, autoimmune thyroid disease and viral myocarditis. The activation of those pathways seems to be related to immune reactions commonly observed after HSCT. Our results contribute to better understanding of the genomic background of the immunologic complications of HSCT.

overall_design: Microarray comparison of genome expression in blood mononuclear cells between two groups of children (pre and post HSCT)

first: Miroslaw

last: Bik-Multanowski


title: Photoperiodic induction of gene expression in Drosophila.

status: Public on Jul 18 2014

city: Leicester

first: Eran

last: Tauber

address: University Road

country: United Kingdom

institute: University of Leicester

summary:
Young Drosophila females respond to low temperature and short photoperiod by developmental arrest of the ovaries.   This form of reproductive diapause is a winter adaptation.
Here we used Affymetrix microarrays to study global expression in female heads associated with long vs. short day.

overall_design: Within 8 hr after eclosion, flies were transferred to vials and maintained in light boxes illuminated by a 4W fluorescent bulb at 12.5°C under either long (16 hr) or short (10 hr) photoperiod. After for 12 days, femlaes were collected 4 hr after lights-on (ZT4).


title: Differential expression of microRNA associated with photoperiod in Drosophila

summary: In Drosophila, the rapid shortening of the day during the autumn induces an arrest of ovarian development (diapause) in females.   The molecular basis underlying this photoperiodic seasonal response is yet unknown.   Here we analyse the global expression changes in transcripts and microRNA (miRNA) associated with photoperiodic diapause response, focusing on the head transcriptome as the putative location of the photoperiodic timer.

platform_organism:
Caenorhabditis elegans
Drosophila melanogaster
Danio rerio
Homo sapiens
Mus musculus
Rattus norvegicus

overall_design: Two-condition experiment, flies in long vs. short day. Biological replicates: 4 for each condition (ca. 100 fly heads each). Three replicates per array. A dye-swap  was carried with two of the samples.

first: Mirko

last: Pegoraro


title: Biomarker discovery in non-small cell lung cancer: integrating gene expression profiling, meta-analysis and tissue microarray validation

status: Public on Oct 12 2012

city: Uppsala

first: Patrick

last: Micke

address: Rudbeck laboratory

country: Sweden

institute: Uppsala University

summary:
Background: Global gene expression profiling has been widely used in lung cancer research to identify clinically relevant molecular subtypes as well as to predict prognosis and therapy response. So far, the value of these multi-gene signatures in clinical practice is unclear and the biological importance of individual genes is difficult to assess as the published signatures virtually do not overlap.

Methods: Here we describe a novel single institute cohort, including 196 non-small lung cancer (NSCLC) cases with clinical information and long-term follow-up, which was used as a training set to screen for single genes with prognostic impact. The top 450 gene probe sets identified using a univariate Cox regression model (significance level p<0.01) were tested in a meta-analysis including five publicly available independent lung cancer cohorts (n=860).

Results: The meta-analysis revealed that 17 probe sets were significantly associated with survival (p<0.0005) with a false discovery rate of 1%. The prognostic impact of one of these genes, the cell adhesion molecule 1 (CADM1), was confirmed by use of immunohistochemistry on a tissue microarray including 355 NSCLC samples. Low CADM1 protein expression was associated with shorter survival (p=0.028), with particular influence in the adenocarcinoma patient subgroup (p=0.002).

Conclusions: We were able to validate single genes with independent prognostic impact using a novel NSCLC cohort together with a meta-analysis approach. CADM1 was identified as an immunohistochemical marker with a potential application in clinical diagnostics.

overall_design: Fresh frozen tissue of 196 consecutive NSCLC patients, operated between 1995 and 2005 were analyzed using Affymetrix microarrays HG-U133-Plus2. Clinical data were retrieved from the regional lung cancer registry.

first: Johan

last: Botling

first: Karolina

last: Edlund

first: Miriam

last: Lohr

first: Birte

last: Hellwig

first: Lars

last: Holmberg

first: Mats

last: Lambe

first: Anders

last: Berglund

first: Simon

last: Ekman

first: Michael

last: Bergkvist

first: Fredrik

last: Pontén

first: André

last: König

first: Jörg

last: Rahnenführer

first: Jan

last: Hengstler

first: Maria

last: Planck


title: Expression data from human SEMDJL1 skin fibroblasts with recessive B3GALT6 mutations (GalT-II deficiency)

status: Public on Jun 10 2014

first: Nicola

last: Chiarelli

summary:
To screen for candidate genes that may contribute to the pathogenesis of GalT-II deficiency. Transcriptome-wide expression profiling using the Affymetrix Gene 1.0 ST platform comparing the gene expression patterns of skin fibroblasts of the two affected sisters with those of three healthy individuals.
Abstract: Mutations in B3GALT6, encoding the galactosyltransferase II (GalT-II) involved in the synthesis of the glycosaminoglycan (GAG) linkage region of proteoglycans (PGs), have recently been associated with a spectrum of connective tissue disorders, including spondyloepimetaphyseal dysplasia with joint laxity type 1 (SEMDJL1) and Ehlers–Danlos-like syndrome. Here, we report on two sisters compound heterozygous for two novel B3GALT6 mutations that presented with severe short stature and progressive kyphoscoliosis, joint hypermobility and laxity, hyperextensible skin, platyspondyly, short ilia, and elbow malalignment. Microarray-based transcriptome analysis revealed the differential expression of several genes encoding extracellular matrix (ECM) structural components, including COMP, SPP1, COL5A1, and COL15A1, enzymes involved in GAG synthesis and in ECM remodeling, such as CSGALNACT1, CHPF, LOXL3, and STEAP4, signaling transduction molecules of the TGFβ/BMP pathway, i.e., GDF6, GDF15, and BMPER, and transcription factors of the HOX and LIM families implicated in skeletal and limb development. Immunofluorescence analyses confirmed the down-regulated expression of some of these genes, in particular of the cartilage oligomeric matrix protein and osteopontin, encoded by COMP and SPP1, respectively, and showed the predominant reduction and disassembly of the heparan sulfate specific GAGs, as well as of the PG perlecan and type III and V collagens. The key role of GalT-II in GAG synthesis and the crucial biological functions of PGs are consistent with the perturbation of many physiological functions that are critical for the correct architecture and homeostasis of various connective tissues, including skin, bone, cartilage, tendons, and ligaments, and generates the wide phenotypic spectrum of GalT-II-deficient patients.

overall_design: Comparison between two SEMDJL1 human fibroblasts and three healthy individuals.


title: Gene expression in Drosophila female heads during diapause.

first: Eran

last: Tauber

summary:
Young Drosophila females respond to low temperature and short photoperiod by developmental arrest of the ovaries.  This form of reproductive diapause is a winter adaptation.
Here we used Affymetrix microarrays to study global expression in female heads associated with diapause.

overall_design: Within 8 hr after eclosion, flies were transferred to vials and maintained in light boxes illuminated by a 4W fluorescent bulb at 12.5°C under light-dark cycle (LD) 14:10. After 12 days, flies were dissected and ovaries status (diapause vs. non-diapause) was determined.


title: Gene expression changes in the course of normal brain aging are sexually dimorphic

status: Public on Oct 09 2008

city: Irvine

first: Nicole

middle: Claudia

last: Berchtold

state: CA

address: 1226 Gillespie Neuroscience Facility

department: Institute for Memory Impairments and Neurological Disorders

institute: UC Irvine

summary:
This dataset of cognitively normal controls is a subset of the GSE48350 dataset, which additionally contains microarray data from AD brains.

Gene expression profiles were assessed in the hippocampus (HC), entorhinal cortex (EC), superior frontal gyrus (SG), and postcentral gyrus (PCG) across the lifespan of 63 cognitively intact individuals from 20-99 years old.  New perspectives on the global gene changes that are associated with brain aging emerged, revealing two overarching concepts.  First, different regions of the forebrain exhibited substantially different gene profile changes with age.  For example, comparing equally powered groups, 5,029 probe sets were significantly altered with age (20-59 vs. 60-99) in the superior frontal gyrus, compared with 1,110 in the entorhinal cortex.  Prominent change occurred in the 6th-7th decades across cortical regions, suggesting that this period is a critical transition point in brain aging, particularly in males.  Second, clear gender differences in brain aging were evident across the lifespan, suggesting that the brain undergoes sexually dimorphic changes in gene expression not only in development but also in later life.  Globally across all brain regions, males showed more gene change than females.  Further, Gene Ontology analysis revealed that different categories of genes were predominantly affected in males vs. females.  Notably, the male brain was characterized by global decreased catabolic and anabolic capacity with aging, with downregulated genes heavily enriched in energy production and protein synthesis/transport categories.  Increased immune activation was a prominent feature of aging in both sexes, with more widespread activation in the female brain.  These data open new opportunities to explore age-dependent changes in gene expression that set the balance between neurodegeneration and compensatory mechanisms in the brain, and suggest that this balance is set differently in males and females, an intriguing and novel idea.

Keywords: Normal gene expression patterns across the adult lifespan

overall_design:
Postmortem brain tissue from 4 brain regions [hippocampus (HC), entorhinal cortex (EC), superior frontal gyrus (SG), and postcentral gyrus (PCG)] of cognitively intact humans (age 20-99) was collected from ADRC brain banks.  Cases were preferentially selected where 3 or more brain regions were available. HgU133plus2.0 microarray chips were used to profile gene expression.
Note that this dataset of cognitively normal controls is a subset of the GSE48350 dataset, which contains data from these cognitively normal controls, and from AD cases.  The control and AD cases were processed simultaneously  (tissue collection, RNA extraction, microarray processing).

first: David

middle: H

last: Cribbs

first: Paul

last: Coleman

first: Joseph

last: Rogers

first: Elizabeth

last: Head

first: Ronald

last: Kim

first: Tom

last: Beach

first: Carol

last: Miller

first: Juan

last: Troncoso

first: John

middle: Q

last: Trojanowski

first: H

last: Zielke

first: Carl

middle: W

last: Cotman


title: Alzheimer's Disease Dataset

status: Public on Apr 21 2014

summary:
This dataset contains microarray data from normal controls (aged 20-99 yrs) and Alzheimer's disease cases, from 4 brain regions: hippocampus, entorhinal cortex, superior frontal cortex, post-central gyrus.   Changes in expression of synaptic and immune related genes were analyzed, investigating age-related changes and AD-related changes, and  region-specific patterns of change.
These AD cases were processed simultaneously with the control cases (young and aged) included in GSE11882 (GSE11882 dataset contains data exclusively from normal control brains).

overall_design: Postmortem brain tissue was collected from ADRC brain banks. Cases were preferentially selected where 3 or more brain regions were available.  Cases labeled "indiv" refers to normal controls (young and aged), and AD cases are labeled "AD".  These AD cases were processed simultaneously with the control cases (young and aged) included in GSE11882 (GSE11882 dataset contains data exclusively from normal control brains).

first: N

first: C


title: Endothelial and Alveolar Rhabdomyosarcoma mRNA expression

status: Public on Dec 31 2013

city: Minneapolis

first: Aaron

last: Sarver

state: MN

address: 425 East River Road

department: Masonic Cancer Center

institute: University of Minnesota

summary: Frozen skeletal muscle, tumor adjacent skeletal muscle, Endothelial Rhabdomyosarcoma (ERMS) and Alveolar Rhabdomyosarcoma (ARMS)  samples were profiled on Illumina bead array.

overall_design: Total RNA from primary resected samples were profiled to allow comparison of 1) normal skeletal muscle tissue with RMS samples and 2) ARMS with ERMS tumors.


title: Analyzing primary Hodgkin and Reed-Sternberg cells to capture the molecular and cellular pathogenesis of classical Hodgkin lymphoma

status: Public on Sep 17 2012

city: Frankfurt

first: Claudia

last: Döring

address: Theodor Stern Kai 7

department: Medical School, Institite of Pathology

institute: Johann Wolfgang-Goethe University Frankfurt a.M.

summary: The pathogenesis of classical Hodgkin lymphoma (cHL), the most common lymphoma in the young, is still enigmatic, largely because its Hodgkin and Reed-Sternberg (HRS) tumor cells are rare in the involved lymph node and therefore difficult to analyze. Here, by overcoming this technical challenge and performing for the first time a genome-wide transcriptional analysis of microdissected HRS cells in comparison to other B-cell lymphomas, cHL lines and normal B-cell subsets, we show that they differ extensively from the usually studied cHL cell lines, that the lost B-cell identity of cHLs is not linked to the acquisition of a plasma cell-like gene expression program, and that Epstein-Barr virus infection of HRS cells has a minor transcriptional influence on the established cHL clone. Moreover, although cHL appears a distinct lymphoma entity overall, HRS cells of its histological subtypes diverged in their similarity to other related lymphomas. Unexpectedly, we identified two molecular subgroups of cHL associated to differential strengths of the transcription factor activity of the NOTCH1, MYC and IRF4 proto-oncogenes. Finally, HRS cells display deregulated expression of several genes potentially highly relevant to lymphoma pathogenesis, including silencing of the apoptosis-inducer BIK and of INPP5D, an inhibitor of the PI3K-driven oncogenic pathway.

overall_design: The present study complements the GSE12453 and GSE14879 records by adding the following 10 samples: 5 primary tumor samples and 5 cell line samples. The 5 primary tumor samples represent 1000-2000 neoplastic cells microdissected from frozen biopsies of 5 cases of primary mediastinal B-cell lymphoma (PMBL). The 5 cell line samples represent 500-1000 living neoplastic cells isolated by fluorescence-activated cell sorting from growing cultures of the classical Hodgkin lymphoma (cHL) cell lines L1236, L428, KMH2 and HDLM2 and the lymphocyte-predominant Hodgkin lymphoma (lpHL) cell line DEV.

first: Enrico

last: Tiacci