title: EV55, ddm1 seedlings H3mK4

description:
ddm1 seedlings, 9 days
Chromatin immunoprecipitation with antibodies raised against dimethyl histone-H3 lysine-4 (H3mK4) (Upstate Biotechnology) was performed as previously described in Gendrel et al. (2002) on material from nine-day-old seedlings, except that the immunoprecipitated (IP) DNA was resuspended in 15ul H20. Seven l from each sample (H3mK9, no antibody control, and input DNA) was used for random amplification. The DNA amplification was performed as described at <http://www.microarrays.org>, except that after Round A (Sequenase step), we carried out only a single Round B of 35 cycles. The DNA obtained was quantified and resolved on a 1.5% agarose gel to check that the range of sizes was between 500bp and ~1.5kb.
1.5ug from each sample was labeled with Cy5 (H3mK4) and Cy3 (Total) using the Megaprime DNA labeling kit (Amersham Pharmacia). Labeled DNA samples were then mixed, purified, and concentrated using Microcon YM-30 columns. The volume was brought to 25ul in a final concentration of 4X SSC, 0.2% SDS.
Hybridizations were carried out at 65C for approximately 15 hrs. Slides were washed in 1X SSC, 0.2% SDS once and 0.2X SSC twice.
Slides were scanned with a GenePix 4000A scanner (Axon Instruments). We quantified the fluorescence with the GenePix Pro software.
Sample_series_id: EV55+56


title: EV49, Wild-type seedlings H3mK4

status: Public on Jun 12 2004

type: genomic

city: Austin

first: Matthew

middle: Wayne

last: Vaughn

state: TX

address: 10100 Burnet Rd

department: Texas Advanced Computing Center

country: USA

institute: University of Texas at Austin

description:
Wild-type seedlings, 9 days
Chromatin immunoprecipitation with antibodies raised against dimethyl histone-H3 lysine-4 (H3mK4) (Upstate Biotechnology) was performed as previously described in Gendrel et al. (2002) on material from nine-day-old seedlings, except that the immunoprecipitated (IP) DNA was resuspended in 15ul H20. Seven l from each sample (H3mK9, no antibody control, and input DNA) was used for random amplification. The DNA amplification was performed as described at <http://www.microarrays.org>, except that after Round A (Sequenase step), we carried out only a single Round B of 35 cycles. The DNA obtained was quantified and resolved on a 1.5% agarose gel to check that the range of sizes was between 500bp and ~1.5kb.
1.5ug from each sample was labeled with Cy5 (H3mK4) and Cy3 (Total) using the Megaprime DNA labeling kit (Amersham Pharmacia). Labeled DNA samples were then mixed, purified, and concentrated using Microcon YM-30 columns. The volume was brought to 25ul in a final concentration of 4X SSC, 0.2% SDS.
Hybridizations were carried out at 65C for approximately 15 hrs. Slides were washed in 1X SSC, 0.2% SDS once and 0.2X SSC twice.
Slides were scanned with a GenePix 4000A scanner (Axon Instruments). We quantified the fluorescence with the GenePix Pro software.
Sample_series_id: EV49+50

molecule: genomic DNA

organism: Arabidopsis thaliana

source_name: H3mK4 ChIP wild-type DNA

source_name: Total ChIP wild-type DNA


title: EV105, ddm1 seedlings H3mK4

description:
ddm1 seedlings, 9 days
Chromatin immunoprecipitation with antibodies raised against dimethyl histone-H3 lysine-4 (H3mK4) (Upstate Biotechnology) was performed as previously described in Gendrel et al. (2002) on material from nine-day-old seedlings, except that the immunoprecipitated (IP) DNA was resuspended in 15ul H20. Seven l from each sample (H3mK4, no antibody control, and input DNA) was used for random amplification. The DNA amplification was performed as described at <http://www.microarrays.org>, except that after Round A (Sequenase step), we carried out only a single Round B of 35 cycles. The DNA obtained was quantified and resolved on a 1.5% agarose gel to check that the range of sizes was between 500bp and ~1.5kb.
1.5ug from each sample was labeled with Cy5 (H3mK4) and Cy3 (Total) using the Megaprime DNA labeling kit (Amersham Pharmacia). Labeled DNA samples were then mixed, purified, and concentrated using Microcon YM-30 columns. The volume was brought to 25ul in a final concentration of 4X SSC, 0.2% SDS.
Hybridizations were carried out at 65C for approximately 15 hrs. Slides were washed in 1X SSC, 0.2% SDS once and 0.2X SSC twice.
Slides were scanned with a GenePix 4000A scanner (Axon Instruments). We quantified the fluorescence with the GenePix Pro software.
Sample_series_id: EV104+105

source_name: H3mK4 ChIP ddm1 DNA

source_name: Total ChIP ddm1 DNA


title: EV110, ddm1 seedlings H3mK9

description:
Wild-type seedlings, 9 days
Chromatin immunoprecipitation with antibodies raised against dimethyl histone-H3 lysine-9 (H3mK9) (Upstate Biotechnology) was performed as previously described in Gendrel et al. (2002) on material from nine-day-old seedlings, except that the immunoprecipitated (IP) DNA was resuspended in 15ul H20. Seven l from each sample (H3mK9, no antibody control, and input DNA) was used for random amplification. The DNA amplification was performed as described at <http://www.microarrays.org>, except that after Round A (Sequenase step), we carried out only a single Round B of 35 cycles. The DNA obtained was quantified and resolved on a 1.5% agarose gel to check that the range of sizes was between 500bp and ~1.5kb.
1.5ug from each sample was labeled with Cy5 (H3mK9) and Cy3 (Total) using the Megaprime DNA labeling kit (Amersham Pharmacia). Labeled DNA samples were then mixed, purified, and concentrated using Microcon YM-30 columns. The volume was brought to 25ul in a final concentration of 4X SSC, 0.2% SDS.
Hybridizations were carried out at 65C for approximately 15 hrs. Slides were washed in 1X SSC, 0.2% SDS once and 0.2X SSC twice.
Slides were scanned with a GenePix 4000A scanner (Axon Instruments). We quantified the fluorescence with the GenePix Pro software.
Sample_series_id: EV110+111

source_name: H3mK9 ChIP ddm1 DNA


title: EV50, Wild-type seedlings H3mK4

description:
Wild-type seedlings, 9 days
Chromatin immunoprecipitation with antibodies raised against dimethyl histone-H3 lysine-4 (H3mK4) (Upstate Biotechnology) was performed as previously described in Gendrel et al. (2002) on material from nine-day-old seedlings, except that the immunoprecipitated (IP) DNA was resuspended in 15ul H20. Seven l from each sample (H3mK9, no antibody control, and input DNA) was used for random amplification. The DNA amplification was performed as described at <http://www.microarrays.org>, except that after Round A (Sequenase step), we carried out only a single Round B of 35 cycles. The DNA obtained was quantified and resolved on a 1.5% agarose gel to check that the range of sizes was between 500bp and ~1.5kb.
1.5ug from each sample was labeled with Cy3 (H3mK4) and Cy5 (Total) using the Megaprime DNA labeling kit (Amersham Pharmacia). Labeled DNA samples were then mixed, purified, and concentrated using Microcon YM-30 columns. The volume was brought to 25ul in a final concentration of 4X SSC, 0.2% SDS.
Hybridizations were carried out at 65C for approximately 15 hrs. Slides were washed in 1X SSC, 0.2% SDS once and 0.2X SSC twice.
Slides were scanned with a GenePix 4000A scanner (Axon Instruments). We quantified the fluorescence with the GenePix Pro software.
Sample_series_id: EV49+50


title: EV109, Wild-type seedlings H3mK9

description:
Wild-type seedlings, 9 days
Chromatin immunoprecipitation with antibodies raised against dimethyl histone-H3 lysine-9 (H3mK9) (Upstate Biotechnology) was performed as previously described in Gendrel et al. (2002) on material from nine-day-old seedlings, except that the immunoprecipitated (IP) DNA was resuspended in 15ul H20. Seven l from each sample (H3mK9, no antibody control, and input DNA) was used for random amplification. The DNA amplification was performed as described at <http://www.microarrays.org>, except that after Round A (Sequenase step), we carried out only a single Round B of 35 cycles. The DNA obtained was quantified and resolved on a 1.5% agarose gel to check that the range of sizes was between 500bp and ~1.5kb.
1.5ug from each sample was labeled with Cy3 (H3mK9) and Cy5 (Total) using the Megaprime DNA labeling kit (Amersham Pharmacia). Labeled DNA samples were then mixed, purified, and concentrated using Microcon YM-30 columns. The volume was brought to 25ul in a final concentration of 4X SSC, 0.2% SDS.
Hybridizations were carried out at 65C for approximately 15 hrs. Slides were washed in 1X SSC, 0.2% SDS once and 0.2X SSC twice.
Slides were scanned with a GenePix 4000A scanner (Axon Instruments). We quantified the fluorescence with the GenePix Pro software.
Sample_series_id: EV108+109

source_name: H3mK9 ChIP wild-type DNA


title: EV107, WT seedlings H3mK4

description:
WT seedlings, 9 days
Chromatin immunoprecipitation with antibodies raised against dimethyl histone-H3 lysine-4 (H3mK4) (Upstate Biotechnology) was performed as previously described in Gendrel et al. (2002) on material from nine-day-old seedlings, except that the immunoprecipitated (IP) DNA was resuspended in 15ul H20. Seven l from each sample (H3mK4, no antibody control, and input DNA) was used for random amplification. The DNA amplification was performed as described at <http://www.microarrays.org>, except that after Round A (Sequenase step), we carried out only a single Round B of 35 cycles. The DNA obtained was quantified and resolved on a 1.5% agarose gel to check that the range of sizes was between 500bp and ~1.5kb.
1.5ug from each sample was labeled with Cy3 (H3mK4) and Cy5 (Total) using the Megaprime DNA labeling kit (Amersham Pharmacia). Labeled DNA samples were then mixed, purified, and concentrated using Microcon YM-30 columns. The volume was brought to 25ul in a final concentration of 4X SSC, 0.2% SDS.
Hybridizations were carried out at 65C for approximately 15 hrs. Slides were washed in 1X SSC, 0.2% SDS once and 0.2X SSC twice.
Slides were scanned with a GenePix 4000A scanner (Axon Instruments). We quantified the fluorescence with the GenePix Pro software.
Sample_series_id: EV106+107

source_name: Total ChIP WT DNA

source_name: H3mK4 ChIP WT DNA


title: Caco-2, Flagellin 3

status: Public on Jun 16 2004

type: RNA

city: Epalinges

first: Pascale

last: Anderle

country: Switzerland

institute: ISREC

description: Caco-2 cells were seeded on Transwell filters, cultured for 14 days, and then treated for 4 h with flagellin

molecule: total RNA

organism: Homo sapiens

source_name: 14 day old Caco-2 cells


title: VC136, wild-type seedlings DNA methylation

description:
wild-type seedlings, 9 days old
Genomic DNA was isolated from wild-type plants using urea extraction buffer according to standard protocols. 20 ug of DNA was sheared to an average size of 3kb using nebulization in a volume of 2ml with a final concentration of 50mM Tris-HCl pH: 8.0, 15mM MgCl2, and 25% glycerol. DNA was then ethanol precipitated and resuspended in 200ul H20. The DNA was split equally into two samples of 100ul each, one of which served as the  untreated  sample. The second DNA sample, serving as the  treated  sample, was then restricted to completion with McrBC in a 300ul volume according to the manufacturer's protocol.
Following heat inactivation of McrBC, both the  untreated  and  treated  samples were ethanol precipitated and resuspended in 35ul H20 each. Each sample was then resolved on a 1% agarose gel, and DNA fragments larger than 1kb were gel-purified and eluted in 120ul H20 using Qiagen Qiaquick gel extraction spin columns according to the manufacturer's protocol.
45ul of  untreated and 45ul of treated DNA was labeled with Cy5 or Cy3, respectively, using the Megaprime DNA labeling kit (Amersham Pharmacia). Labeled DNA samples were then mixed, purified, and concentrated using Microcon YM-30 columns. The volume was brought to 25ul in a final concentration of 4X SSC, 0.2% SDS.
Hybridizations were carried out at 65C for approximately 15 hrs. Slides were washed in 1X SSC, 0.2% SDS once and 0.2X SSC twice.
Slides were scanned with a GenePix 4000A scanner (Axon Instruments). We quantified the fluorescence with the GenePix Pro software.
Sample_series_id: VC134+136

source_name: Nebulized wild-type genomic DNA

source_name: Nebulized, McrBC-treated wild-type genomic DNA


title: EV104, ddm1 seedlings H3mK4

description:
ddm1 seedlings, 9 days
Chromatin immunoprecipitation with antibodies raised against dimethyl histone-H3 lysine-4 (H3mK4) (Upstate Biotechnology) was performed as previously described in Gendrel et al. (2002) on material from nine-day-old seedlings, except that the immunoprecipitated (IP) DNA was resuspended in 15ul H20. Seven l from each sample (H3mK4, no antibody control, and input DNA) was used for random amplification. The DNA amplification was performed as described at <http://www.microarrays.org>, except that after Round A (Sequenase step), we carried out only a single Round B of 35 cycles. The DNA obtained was quantified and resolved on a 1.5% agarose gel to check that the range of sizes was between 500bp and ~1.5kb.
1.5ug from each sample was labeled with Cy3 (H3mK4) and Cy5 (Total) using the Megaprime DNA labeling kit (Amersham Pharmacia). Labeled DNA samples were then mixed, purified, and concentrated using Microcon YM-30 columns. The volume was brought to 25ul in a final concentration of 4X SSC, 0.2% SDS.
Hybridizations were carried out at 65C for approximately 15 hrs. Slides were washed in 1X SSC, 0.2% SDS once and 0.2X SSC twice.
Slides were scanned with a GenePix 4000A scanner (Axon Instruments). We quantified the fluorescence with the GenePix Pro software.
Sample_series_id: EV104+105


title: EV59, ddm1 seedlings H3mK9

description:
ddm1 seedlings, 9 days
Chromatin immunoprecipitation with antibodies raised against dimethyl histone-H3 lysine-9 (H3mK9) (Upstate Biotechnology) was performed as previously described in Gendrel et al. (2002) on material from nine-day-old seedlings, except that the immunoprecipitated (IP) DNA was resuspended in 15ul H20. Seven l from each sample (H3mK9, no antibody control, and input DNA) was used for random amplification. The DNA amplification was performed as described at <http://www.microarrays.org>, except that after Round A (Sequenase step), we carried out only a single Round B of 35 cycles. The DNA obtained was quantified and resolved on a 1.5% agarose gel to check that the range of sizes was between 500bp and ~1.5kb.
1.5ug from each sample was labeled with Cy3 (H3mK9) and Cy5 (Total) using the Megaprime DNA labeling kit (Amersham Pharmacia). Labeled DNA samples were then mixed, purified, and concentrated using Microcon YM-30 columns. The volume was brought to 25ul in a final concentration of 4X SSC, 0.2% SDS.
Hybridizations were carried out at 65C for approximately 15 hrs. Slides were washed in 1X SSC, 0.2% SDS once and 0.2X SSC twice.
Slides were scanned with a GenePix 4000A scanner (Axon Instruments). We quantified the fluorescence with the GenePix Pro software.
Sample_series_id: EV59+60


title: UN-acute exposure

status: Public on Jun 10 2004

type: SAGE

anchor: NlaIII

city: Montpellier

first: Magali

last: Taulan

country: France

institute: INSERM

description: kidney from 9- to 11-week-old male C57BL/6 mice, exposed to uranyl nitrate

organism: Mus musculus

source_name: kidney mouse


title: UN-chronic exposure

description: kidney from 25- to 28-week-old male C57BL/6 mice, daily exposed to uranyl nitrate


title: normal kidney

description: kidney from 25- to 28-week-old male C57BL/6 mice

source_name: control mouse kidney


title: VC133, ddm1 seedlings DNA methylation

description:
ddm1 seedlings, 9 days old
Genomic DNA was isolated from ddm1 plants using urea extraction buffer according to standard protocols. 20 ug of DNA was sheared to an average size of 3kb using nebulization in a volume of 2ml with a final concentration of 50mM Tris-HCl pH: 8.0, 15mM MgCl2, and 25% glycerol. DNA was then ethanol precipitated and resuspended in 200ul H20. The DNA was split equally into two samples of 100ul each, one of which served as the  untreated  sample. The second DNA sample, serving as the  treated  sample, was then restricted to completion with McrBC in a 300ul volume according to the manufacturer s protocol.
Following heat inactivation of McrBC, both the  untreated  and  treated  samples were ethanol precipitated and resuspended in 35ul H20 each. Each sample was then resolved on a 1% agarose gel, and DNA fragments larger than 1kb were gel-purified and eluted in 120ul H20 using Qiagen Qiaquick gel extraction spin columns according to the manufacturer s protocol.
45ul of  untreated  and 45ul of  treated  DNA was labeled with Cy5 or Cy3, respectively, using the Megaprime DNA labeling kit (Amersham Pharmacia). Labeled DNA samples were then mixed, purified, and concentrated using Microcon YM-30 columns. The volume was brought to 25ul in a final concentration of 4X SSC, 0.2% SDS.
Hybridizations were carried out at 65C for approximately 15 hrs. Slides were washed in 1X SSC, 0.2% SDS once and 0.2X SSC twice.
Slides were scanned with a GenePix 4000A scanner (Axon Instruments). We quantified the fluorescence with the GenePix Pro software.
Sample_series_id: VC133+137

source_name: Nebulized ddm1 genomic DNA

source_name: Nebulized, McrBC-treated ddm1 genomic DNA


title: VC131, ddm1 seedlings DNA methylation

description:
ddm1 plants, 9 days old
Genomic DNA was isolated from ddm1 plants using urea extraction buffer according to standard protocols. 20 ug of DNA was sheared to an average size of 3kb using nebulization in a volume of 2ml with a final concentration of 50mM Tris-HCl pH: 8.0, 15mM MgCl2, and 25% glycerol. DNA was then ethanol precipitated and resuspended in 200ul H20. The DNA was split equally into two samples of 100ul each, one of which served as the  untreated  sample. The second DNA sample, serving as the  treated  sample, was then restricted to completion with McrBC in a 300ul volume according to the manufacturer s protocol.
Following heat inactivation of McrBC, both the  untreated  and  treated  samples were ethanol precipitated and resuspended in 35ul H20 each. Each sample was then resolved on a 1% agarose gel, and DNA fragments larger than 1kb were gel-purified and eluted in 120ul H20 using Qiagen Qiaquick gel extraction spin columns according to the manufacturer s protocol.
45ul of  untreated  and 45ul of  treated  DNA was labeled with Cy5 or Cy3, respectively, using the Megaprime DNA labeling kit (Amersham Pharmacia). Labeled DNA samples were then mixed, purified, and concentrated using Microcon YM-30 columns. The volume was brought to 25ul in a final concentration of 4X SSC, 0.2% SDS.
Hybridizations were carried out at 65C for approximately 15 hrs. Slides were washed in 1X SSC, 0.2% SDS once and 0.2X SSC twice.
Slides were scanned with a GenePix 4000A scanner (Axon Instruments). We quantified the fluorescence with the GenePix Pro software.
Sample_series_id: VC131+135


title: VC134, wild-type seedlings DNA methylation

description:
wild-type seedlings, 9 days old
Genomic DNA was isolated from wild-type plants using urea extraction buffer according to standard protocols. 20 ug of DNA was sheared to an average size of 3kb using nebulization in a volume of 2ml with a final concentration of 50mM Tris-HCl pH: 8.0, 15mM MgCl2, and 25% glycerol. DNA was then ethanol precipitated and resuspended in 200ul H20. The DNA was split equally into two samples of 100ul each, one of which served as the untreated sample. The second DNA sample, serving as the treated sample, was then restricted to completion with McrBC in a 300ul volume according to the manufacturer s protocol.
Following heat inactivation of McrBC, both the untreated and treated samples were ethanol precipitated and resuspended in 35ul H20 each. Each sample was then resolved on a 1% agarose gel, and DNA fragments larger than 1kb were gel-purified and eluted in 120ul H20 using Qiagen Qiaquick gel extraction spin columns according to the manufacturer s protocol.
45ul of untreated and 45ul of treated DNA was labeled with Cy3 or Cy5, respectively, using the Megaprime DNA labeling kit (Amersham Pharmacia). Labeled DNA samples were then mixed, purified, and concentrated using Microcon YM-30 columns. The volume was brought to 25ul in a final concentration of 4X SSC, 0.2% SDS.
Hybridizations were carried out at 65C for approximately 15 hrs. Slides were washed in 1X SSC, 0.2% SDS once and 0.2X SSC twice.
Slides were scanned with a GenePix 4000A scanner (Axon Instruments). We quantified the fluorescence with the GenePix Pro software.
Sample_series_id: VC134+136


title: VC137, ddm1 seedlings DNA methylation

description:
ddm1 seedlings, 9 days old
Genomic DNA was isolated from ddm1 plants using urea extraction buffer according to standard protocols. 20 ug of DNA was sheared to an average size of 3kb using nebulization in a volume of 2ml with a final concentration of 50mM Tris-HCl pH: 8.0, 15mM MgCl2, and 25% glycerol. DNA was then ethanol precipitated and resuspended in 200ul H20. The DNA was split equally into two samples of 100ul each, one of which served as the  untreated  sample. The second DNA sample, serving as the  treated  sample, was then restricted to completion with McrBC in a 300ul volume according to the manufacturer s protocol.
Following heat inactivation of McrBC, both the  untreated  and  treated  samples were ethanol precipitated and resuspended in 35ul H20 each. Each sample was then resolved on a 1% agarose gel, and DNA fragments larger than 1kb were gel-purified and eluted in 120ul H20 using Qiagen Qiaquick gel extraction spin columns according to the manufacturer s protocol.
45ul of  untreated  and 45ul of  treated  DNA was labeled with Cy3 or Cy5, respectively, using the Megaprime DNA labeling kit (Amersham Pharmacia). Labeled DNA samples were then mixed, purified, and concentrated using Microcon YM-30 columns. The volume was brought to 25ul in a final concentration of 4X SSC, 0.2% SDS.
Hybridizations were carried out at 65C for approximately 15 hrs. Slides were washed in 1X SSC, 0.2% SDS once and 0.2X SSC twice.
Slides were scanned with a GenePix 4000A scanner (Axon Instruments). We quantified the fluorescence with the GenePix Pro software.
Sample_series_id: VC133+137


title: VC127, Wild-type bolting plants DNA methylation

description:
Wild-type plants, bolting stage
Genomic DNA was isolated from wild-type plants using urea extraction buffer according to standard protocols. 20 g of DNA was sheared to an average size of 3kb using nebulization in a volume of 2ml with a final concentration of 50mM Tris-HCl pH: 8.0, 15mM MgCl2, and 25% glycerol. DNA was then ethanol precipitated and resuspended in 200ul H20. The DNA was split equally into two samples of 100ul each, one of which served as the  untreated  sample. The second DNA sample, serving as the  treated  sample, was then restricted to completion with McrBC in a 300ul volume according to the manufacturer s protocol.
Following heat inactivation of McrBC, both the  untreated  and  treated  samples were ethanol precipitated and resuspended in 35ul H20 each. Each sample was then resolved on a 1% agarose gel, and DNA fragments larger than 1kb were gel-purified and eluted in 120ul H20 using Qiagen Qiaquick gel extraction spin columns according to the manufacturer s protocol.
45ul of  untreated  and 45ul of  treated  DNA was labeled with Cy5 or Cy3, respectively, using the Megaprime DNA labeling kit (Amersham Pharmacia). Labeled DNA samples were then mixed, purified, and concentrated using Microcon YM-30 columns. The volume was brought to 25ul in a final concentration of 4X SSC, 0.2% SDS.
Hybridizations were carried out at 65C for approximately 15 hrs. Slides were washed in 1X SSC, 0.2% SDS once and 0.2X SSC twice.
Slides were scanned with a GenePix 4000A scanner (Axon Instruments). We quantified the fluorescence with the GenePix Pro software.
Sample_series_id: VC121+127


title: VC121, Wild-type bolting plants DNA methylation

description:
Wild-type plants, bolting stage
Genomic DNA was isolated from wild-type plants using urea extraction buffer according to standard protocols. 20 g of DNA was sheared to an average size of 3kb using nebulization in a volume of 2ml with a final concentration of 50mM Tris-HCl pH: 8.0, 15mM MgCl2, and 25% glycerol. DNA was then ethanol precipitated and resuspended in 200ul H20. The DNA was split equally into two samples of 100ul each, one of which served as the  untreated  sample. The second DNA sample, serving as the  treated  sample, was then restricted to completion with McrBC in a 300ul volume according to the manufacturer s protocol.
Following heat inactivation of McrBC, both the  untreated  and  treated  samples were ethanol precipitated and resuspended in 35ul H20 each. Each sample was then resolved on a 1% agarose gel, and DNA fragments larger than 1kb were gel-purified and eluted in 120ul H20 using Qiagen Qiaquick gel extraction spin columns according to the manufacturer s protocol.
45ul of  untreated  and 45ul of  treated  DNA was labeled with Cy3 or Cy5, respectively, using the Megaprime DNA labeling kit (Amersham Pharmacia). Labeled DNA samples were then mixed, purified, and concentrated using Microcon YM-30 columns. The volume was brought to 25ul in a final concentration of 4X SSC, 0.2% SDS.
Hybridizations were carried out at 65C for approximately 15 hrs. Slides were washed in 1X SSC, 0.2% SDS once and 0.2X SSC twice.
Slides were scanned with a GenePix 4000A scanner (Axon Instruments). We quantified the fluorescence with the GenePix Pro software.
Sample_series_id: VC121+127


title: HEK-293_clone H36_exp #2

status: Public on Jun 08 2004

city: Chicago

first: Tatiana

last: Zagranichnaya

state: IL

institute: The University of Chicago

description:
Monoclonal cell line H36 (derived from HEK-293 parental cell culture) with a high level of store-operated Ca2+ entry (SOCE). For selection of clones, HEK-293 cells were loaded with indo-1 and sorted by FACS on the basis of their cyclopiazonic acid (CPA)-stimulated Ca2+ entry. Monoclonal cell lines were selected from the sorted cells and their levels of SOCE confirmed by monitoring thapsigargin-stimulated Ba2+ entry. Total RNA was extracted from cells immediately after removal from their growth environment. RNA was processed and hybridized to the Affymetrix HG-U133A chip.
Keywords = cDNA microarray
Keywords = FACS analysis
Keywords = thapsigargin
Keywords = store-operated Ca2+ entry (SOCE)

source_name: Monoclonal cell line H36 derived from HEK-293 parental cells


title: HEK-293_clone L29_exp #2

description:
Monoclonal cell line L29 (derived from HEK-293 parental cell culture) with a low level of store-operated Ca2+ entry (SOCE). For selection of clones, HEK-293 cells were loaded with indo-1 and sorted by FACS on the basis of their cyclopiazonic acid (CPA)-stimulated Ca2+ entry. Monoclonal cell lines were selected from the sorted cells and their levels of SOCE confirmed by monitoring thapsigargin-stimulated Ba2+ entry. Total RNA was extracted from cells immediately after removal from their growth environment. RNA was processed and hybridized to the Affymetrix HG-U133A chip.
Keywords = cDNA microarray
Keywords = FACS analysis
Keywords = thapsigargin
Keywords = store-operated Ca2+ entry (SOCE)

source_name: Monoclonal cell line L29 derived from HEK-293 parental cells


title: HEK-293_clone H36(duplicate)_exp #2


title: HEK-293_clone L28_exp #2

description:
Monoclonal cell line L28 (derived from HEK-293 parental cell culture) with a low level of store-operated Ca2+ entry (SOCE). For selection of clones, HEK-293 cells were loaded with indo-1 and sorted by FACS on the basis of their cyclopiazonic acid (CPA)-stimulated Ca2+ entry. Monoclonal cell lines were selected from the sorted cells and their levels of SOCE confirmed by monitoring thapsigargin-stimulated Ba2+ entry. Total RNA was extracted from cells immediately after removal from their growth environment. RNA was processed and hybridized to the Affymetrix HG-U133A chip.
Keywords = cDNA microarray
Keywords = FACS analysis
Keywords = thapsigargin
Keywords = store-operated Ca2+ entry (SOCE)

source_name: Monoclonal cell line L28 derived from HEK-293 parental cells


title: HEK-293(duplicate)_exp #2

description:
Gene expression profile of the parental HEK-293 cell population from which clones with hihg or low store-operated calcium entry were selected. Total RNA was extracted from cells immediately after removal from their growth environment. RNA was processed and hybridized to the Affymetrix HG-U133A chip.
Keywords = cDNA microarray
Keywords = FACS analysis
Keywords = thapsigargin
Keywords = store-operated Ca2+ entry (SOCE)

source_name: HEK-293 cells


title: HEK-293_clone L29(duplicate)_exp #2


title: HEK293_clone H15_exp #1

description:
Monoclonal cell line H15 (derived from HEK-293 parental cell culture) with a high level of store-operated Ca2+ entry (SOCE). For selection of clones, HEK-293 cells were loaded with indo-1 and sorted by FACS on the basis of their cyclopiazonic acid (CPA)-stimulated Ca2+ entry. Monoclonal cell lines were selected from the sorted cells and their levels of SOCE confirmed by monitoring thapsigargin-stimulated Ba2+ entry. Total RNA was extracted from cells immediately after removal from its growth environment. RNA was processed and hybridized to the Affymetrix HG-U133A chip.
Keywords = cDNA microarray
Keywords = FACS analysis
Keywords = thapsigargin
Keywords = store-operated Ca2+ entry (SOCE)

source_name: Monoclonal cell line H15 derived from HEK-293 parental cells


title: HEK-293_clone H1(duplicate)_exp #1

description:
Monoclonal cell line H1 (derived from HEK-293 parental cell culture) with a high level of store-operated Ca2+ entry (SOCE). For selection of clones, HEK-293 cells were loaded with indo-1 and sorted by FACS on the basis of their cyclopiazonic acid (CPA)-stimulated Ca2+ entry. Monoclonal cell lines were selected from the sorted cells and their levels of SOCE confirmed by monitoring thapsigargin-stimulated Ba2+ entry. Total RNA was extracted from cells immediately after removal from their growth environment. RNA was processed and hybridized to the Affymetrix HG-U133A chip.

Keywords = cDNA microarray
Keywords = FACS analysis
Keywords = thapsigargin
Keywords = store-operated Ca2+ entry (SOCE)
Keywords = HEK-293

source_name: Monoclonal cell line H1 derived from HEK-293 parental cells


title: HEK293_clone H36_exp #1

description:
Monoclonal cell line H36 (derived from HEK-293 parental cell culture) with a high level of store-operated Ca2+ entry (SOCE). For selection of clones, HEK-293 cells were loaded with indo-1 and sorted by FACS on the basis of their cyclopiazonic acid (CPA)-stimulated Ca2+ entry. Monoclonal cell lines were selected from the sorted cells and their levels of SOCE confirmed by monitoring thapsigargin-stimulated Ba2+ entry. Total RNA was extracted from cells immediately after removal from its growth environment. RNA was processed and hybridized to the Affymetrix HG-U133A chip.
Keywords = cDNA microarray
Keywords = FACS analysis
Keywords = thapsigargin
Keywords = store-operated Ca2+ entry (SOCE)


title: HEK-293_clone L28(duplicate)_exp #2


title: HEK-293_clone H1_exp #1

description:
Monoclonal cell line H1 (derived from HEK-293 parental cell culture) with a high  level of store-operated Ca2+ entry (SOCE). For selection of clones, HEK-293 cells were loaded with indo-1 and sorted by FACS on the basis of their cyclopiazonic acid (CPA)-stimulated Ca2+ entry.  Monoclonal cell lines were selected from the sorted cells and their levels of SOCE confirmed by monitoring thapsigargin-stimulated Ba2+ entry. Total RNA was extracted from cells immediately after removal from its growth environment. RNA was processed and hybridized to the Affymetrix HG-U133A chip.

Keywords = cDNA microarray
Keywords = FACS analysis
Keywords = thapsigargin
Keywords = store-operated Ca2+ entry (SOCE)
Keywords = HEK-293


title: HEK-293_clone H15(duplicate)_exp #1


title: HEK-293_clone L3(duplicate)_exp #1

description:
Monoclonal cell line L3 (derived from HEK-293 parental cell culture) with a low level of store-operated Ca2+ entry (SOCE). For selection of clones, HEK-293 cells were loaded with indo-1 and sorted by FACS on the basis of their cyclopiazonic acid (CPA)-stimulated Ca2+ entry. Monoclonal cell lines were selected from the sorted cells and their levels of SOCE confirmed by monitoring thapsigargin-stimulated Ba2+ entry. Total RNA was extracted from cells immediately after removal from its growth environment. RNA was processed and hybridized to the Affymetrix HG-U133A chip.
Keywords = cDNA microarray
Keywords = FACS analysis
Keywords = thapsigargin
Keywords = store-operated Ca2+ entry (SOCE)

source_name: Monoclonal cell line L3 derived from HEK-293 parental cells


title: HEK-293_exp #1


title: HEK293_clone H36(duplicate)_exp #1


title: HEK-293_clone H39(duplicate)_exp #1

description:
Monoclonal cell line H39 (derived from HEK-293 parental cell culture) with a high level of store-operated Ca2+ entry (SOCE). For selection of clones, HEK-293 cells were loaded with indo-1 and sorted by FACS on the basis of their cyclopiazonic acid (CPA)-stimulated Ca2+ entry. Monoclonal cell lines were selected from the sorted cells and their levels of SOCE confirmed by monitoring thapsigargin-stimulated Ba2+ entry. Total RNA was extracted from cells immediately after removal from its growth environment. RNA was processed and hybridized to the Affymetrix HG-U133A chip.
Keywords = cDNA microarray
Keywords = FACS analysis
Keywords = thapsigargin
Keywords = store-operated Ca2+ entry (SOCE)
Keywords = HEK-293

source_name: Monoclonal cell line H39 derived from HEK-293 parental cells


title: VC135, ddm1 seedlings DNA methylation

description:
ddm1 plants, 9 days old
Genomic DNA was isolated from ddm1 plants using urea extraction buffer according to standard protocols. 20 ug of DNA was sheared to an average size of 3kb using nebulization in a volume of 2ml with a final concentration of 50mM Tris-HCl pH: 8.0, 15mM MgCl2, and 25% glycerol. DNA was then ethanol precipitated and resuspended in 200ul H20. The DNA was split equally into two samples of 100ul each, one of which served as the  untreated  sample. The second DNA sample, serving as the  treated  sample, was then restricted to completion with McrBC in a 300ul volume according to the manufacturer s protocol.
Following heat inactivation of McrBC, both the  untreated  and  treated  samples were ethanol precipitated and resuspended in 35ul H20 each. Each sample was then resolved on a 1% agarose gel, and DNA fragments larger than 1kb were gel-purified and eluted in 120ul H20 using Qiagen Qiaquick gel extraction spin columns according to the manufacturer s protocol.
45ul of  untreated  and 45ul of  treated  DNA was labeled with Cy3 or Cy5, respectively, using the Megaprime DNA labeling kit (Amersham Pharmacia). Labeled DNA samples were then mixed, purified, and concentrated using Microcon YM-30 columns. The volume was brought to 25ul in a final concentration of 4X SSC, 0.2% SDS.
Hybridizations were carried out at 65C for approximately 15 hrs. Slides were washed in 1X SSC, 0.2% SDS once and 0.2X SSC twice.
Slides were scanned with a GenePix 4000A scanner (Axon Instruments). We quantified the fluorescence with the GenePix Pro software.
Sample_series_id: VC131+135


title: HEK-293_clone H24(duplicate)_exp #1

description:
Monoclonal cell line H24 (derived from HEK-293 parental cell culture) with a high level of store-operated Ca2+ entry (SOCE). For selection of clones, HEK-293 cells were loaded with indo-1 and sorted by FACS on the basis of their cyclopiazonic acid (CPA)-stimulated Ca2+ entry. Monoclonal cell lines were selected from the sorted cells and their levels of SOCE confirmed by monitoring thapsigargin-stimulated Ba2+ entry. Total RNA was extracted from cells immediately after removal from its growth environment. RNA was processed and hybridized to the Affymetrix HG-U133A chip.
Keywords = cDNA microarray
Keywords = FACS analysis
Keywords = thapsigargin
Keywords = store-operated Ca2+ entry (SOCE)

source_name: Monoclonal cell line H24 derived from HEK-293 parental cells


title: HEK-293(duplicate)_exp #1


title: HEK-293_clone H24_exp #1


title: HEK-293_clone L3_exp #1


title: HEK-293_exp #2


title: whole tissue section from case 1507

status: Public on Jun 07 2004

city: Carlsbad

first: Xiao-Jun

last: Ma

state: CA

address: 2715 Loker Ave. W.

institute: AviaraDx, Inc.

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=1507;Tumor.type=D;Size=3.7;Grade=3;Nodes=0/40;ER=Pos;PR=Pos;HER2=Neg ;Age=70;DFS=70;Status=non-recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 1507 labeled with Cy5

source_name: human universal reference RNA (Stratagene) labeled with Cy3


title: HEK-293_clone H39_exp #1


title: whole tissue section from case 1437

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=1437;Tumor.type=D;Size=1.7;Grade=2;Nodes=2/22;ER=Pos;PR=Pos;HER2=Neg;Age=67;DFS=89;Status=non-recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 1437 labeled with Cy5


title: whole tissue section from case 1419

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=1419;Tumor.type=D;Size=2.5;Grade=2;Nodes=1/8;ER=Pos;PR=Pos;HER2=Neg;Age=59;DFS=86;Status=non-recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 1419 labeled with Cy5


title: whole tissue section from case 1405

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=1405;Tumor.type=D;Size=1;Grade=3;Nodes=ND;ER=Pos (<10%);PR=Pos;HER2=Neg;Age=81;DFS=95;Status=non-recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 1405 labeled with Cy5


title: whole tissue section from case 1389

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=1389;Tumor.type=D;Size=1.7;Grade=2;Nodes=0/1;ER=Pos;PR=Pos;HER2=Neg;Age=80;DFS=94;Status=non-recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 1389 labeled with Cy5


title: whole tissue section from case 1304

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=1304;Tumor.type=D;Size=2;Grade=3;Nodes=0/14;ER=Pos;PR=Pos;HER2=Neg;Age=57;DFS=20;Status=recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 1304 labeled with Cy5


title: control Kidney

description: kidney from 9- to 11-week-old male C57BL/6 mice

source_name: control mouse Kidney


title: whole tissue section from case 1279

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=1279;Tumor.type=D;Size=2;Grade=2;Nodes=0/12;ER=Pos;PR=Pos;HER2=Neg;Age=68;DFS=102;Status=non-recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 1279 labeled with Cy5


title: whole tissue section from case 1206

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=1206;Tumor.type=D;Size=4.1;Grade=3;Nodes=0/15;ER=Pos;PR=Neg;HER2=Neg;Age=84;DFS=56;Status=recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 1206 labeled with Cy5


title: whole tissue section from case 1218

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=1218;Tumor.type=D;Size=4.5;Grade=1;Nodes=3/16;ER=Pos;PR=Pos;HER2=Neg;Age=62;DFS=10;Status=recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 1218 labeled with Cy5


title: whole tissue section from case 1047

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=1047;Tumor.type=D/L;Size=2.6;Grade=2;Nodes=0/18;ER=Pos;PR=Neg;HER2=Neg;Age=70;DFS=128;Status=non-recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 1047 labeled with Cy5


title: whole tissue section from case 1363

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=1363;Tumor.type=D;Size=2.1;Grade=2;Nodes=0/15;ER=Pos;PR=Pos;HER2=Neg ;Age=56;DFS=114;Status=non-recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 1363 labeled with Cy5


title: whole tissue section from case 871

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=871;Tumor.type=D;Size=4;Grade=3;Nodes=0/16;ER=Pos;PR=Pos;HER2=Neg;Age=61;DFS=6;Status=recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 871 labeled with Cy5


title: whole tissue section from case 1343

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=1343;Tumor.type=D;Size=2.5;Grade=3;Nodes=ND;ER=Pos;PR=Pos;HER2=Neg;Age=79;DFS=21;Status=recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 1343 labeled with Cy5


title: whole tissue section from case 868

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=868;Tumor.type=D;Size=3;Grade=3;Nodes=0/13;ER=Pos;PR=Pos;HER2=Neg;Age=65;DFS=130;Status=non-recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 868 labeled with Cy5


title: whole tissue section from case 1368

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=1368;Tumor.type=D;Size=2.6;Grade=2;Nodes=ND;ER=Pos;PR=Pos;HER2=Neg ;Age=82;DFS=63;Status=non-recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 1368 labeled with Cy5


title: whole tissue section from case 849

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=849;Tumor.type=D;Size=2;Grade=1;Nodes=0/26;ER=Pos;PR=Pos;HER2=Neg;Age=75;DFS=23;Status=recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 849 labeled with Cy5


title: whole tissue section from case 1085

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=1085;Tumor.type=D;Size=4.7;Grade=2;Nodes=0/8;ER=Pos;PR=Pos;HER2=Neg;Age=48;DFS=101;Status=recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 1085 labeled with Cy5

source_name: human universal reference RNA (Stratagene) labeled with Cy3


title: whole tissue section from case 1370

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=1370;Tumor.type=D;Size=2;Grade=2;Nodes=ND;ER=Pos;PR=Pos;HER2=Neg;Age=73;DFS=61;Status=non-recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 1370 labeled with Cy5


title: whole tissue section from case 843

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=843;Tumor.type=D;Size=3.4;Grade=2;Nodes=11/20;ER=Pos;PR=Neg;HER2=Neg;Age=76;DFS=122;Status=recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 843 labeled with Cy5


title: whole tissue section from case 772

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=772;Tumor.type=D;Size=2.5;Grade=2;Nodes=0/18;ER=Pos;PR=Pos;HER2=Neg;Age=68;DFS=69;Status=recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 772 labeled with Cy5


title: whole tissue section from case 1093

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=1093;Tumor.type=D;Size=1;Grade=3;Nodes=1/14;ER=Pos;PR=Pos;HER2=Neg ;Age=66;DFS=85;Status=non-recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 1093 labeled with Cy5


title: whole tissue section from case 856

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=856;Tumor.type=D;Size=1.6;Grade=2;Nodes=0/16;ER=Pos;PR=Pos;HER2=ND;Age=73;DFS=88;Status=non-recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 856 labeled with Cy5


title: whole tissue section from case 823

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=823;Tumor.type=D;Size=2;Grade=2;Nodes=0/6;ER=Pos;PR=Pos;HER2=Neg;Age=51;DFS=69;Status=recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 823 labeled with Cy5


title: whole tissue section from case 1037

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=1037;Tumor.type=D/L;Size=1.5;Grade=2;Nodes=0/4;ER=Pos;PR=Pos;HER2=Neg ;Age=85;DFS=83;Status=non-recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 1037 labeled with Cy5


title: whole tissue section from case 1125

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=1125;Tumor.type=D;Size=2.6;Grade=2;Nodes=0/18;ER=Pos;PR=Neg;HER2=Neg;Age=54;DFS=123;Status=non-recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 1125 labeled with Cy5


title: whole tissue section from case 1045

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=1045;Tumor.type=D;Size=2.5;Grade=3;Nodes=1/12;ER=Pos;PR=Neg;HER2=Pos;Age=73;DFS=121;Status=non-recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 1045 labeled with Cy5


title: whole tissue section from case 1260

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=1260;Tumor.type=D;Size=3.5;Grade=2;Nodes=0/14;ER=Pos;PR=Pos;HER2=Neg;Age=58;DFS=79;Status=non-recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 1260 labeled with Cy5


title: whole tissue section from case 829

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=829;Tumor.type=D;Size=1.2;Grade=2;Nodes=0/9;ER=Pos;PR=Pos;HER2=Neg ;Age=69;DFS=136;Status=non-recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 829 labeled with Cy5


title: whole tissue section from case 878

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=878;Tumor.type=D/L;Size=1.6;Grade=2;Nodes=0/9;ER=Pos;PR=Neg;HER2=Neg;Age=76;DFS=138;Status=non-recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 878 labeled with Cy5


title: whole tissue section from case 763

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=763;Tumor.type=D;Size=1.8;Grade=2;Nodes=0/14;ER=Pos;PR=Pos;HER2=ND;Age=63;DFS=118;Status=non-recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 763 labeled with Cy5


title: whole tissue section from case 1205

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=1205;Tumor.type=D;Size=2.7;Grade=2;Nodes=1/19;ER=Pos;PR=Pos;HER2=Neg ;Age=71;DFS=88;Status=non-recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 1205 labeled with Cy5


title: whole tissue section from case 687

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=687;Tumor.type=D;Size=3.5;Grade=3;Nodes=3/16;ER=Pos;PR=Pos;HER2=Neg;Age=73;DFS=142;Status=non-recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 687 labeled with Cy5


title: whole tissue section from case 749

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=749;Tumor.type=D;Size=1.8;Grade=2;Nodes=2/9;ER=Pos;PR=Neg;HER2=Neg;Age=61;DFS=137;Status=non-recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 749 labeled with Cy5


title: whole tissue section from case 469

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=469;Tumor.type=D;Size=1;Grade=1;Nodes=0/19;ER=Pos;PR=Pos;HER2=Neg ;Age=66;DFS=161;Status=non-recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 469 labeled with Cy5

source_name: human universal reference RNA (Stratagene) labeled with Cy3


title: whole tissue section from case 605

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=605;Tumor.type=D;Size=2.2;Grade=2;Nodes=6/18;ER=Pos;PR=Pos;HER2=Neg;Age=70;DFS=110;Status=non-recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 605 labeled with Cy5


title: whole tissue section from case 662

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=662;Tumor.type=D;Size=1;Grade=3;Nodes=6/11;ER=Pos;PR=Pos;HER2=Neg;Age=79;DFS=27;Status=recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 662 labeled with Cy5


title: whole tissue section from case 356

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=356;Tumor.type=D;Size=1;Grade=2;Nodes=2/20;ER=Pos (<10%);PR=Neg;HER2=Pos;Age=58;DFS=24;Status=recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 356 labeled with Cy5


title: whole tissue section from case 420

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=420;Tumor.type=D/L;Size=2;Grade=3;Nodes=ND;ER=Pos;PR=Pos;HER2=Neg;Age=72;DFS=58;Status=recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 420 labeled with Cy5


title: whole tissue section from case 651

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=651;Tumor.type=D;Size=4.7;Grade=3;Nodes=10/13;ER=Pos;PR=Pos;HER2=Neg;Age=48;DFS=137;Status=recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 651 labeled with Cy5


title: whole tissue section from case 607

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=607;Tumor.type=D;Size=1.2;Grade=2;Nodes=5/14;ER=Pos;PR=Pos;HER2=Neg;Age=76;DFS=114;Status=non-recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 607 labeled with Cy5


title: whole tissue section from case 638

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=638;Tumor.type=D;Size=2;Grade=2;Nodes=1/24;ER=Pos;PR=Pos;HER2=Neg;Age=67;DFS=148;Status=non-recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 638 labeled with Cy5


title: whole tissue section from case 518

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=518;Tumor.type=L;Size=5.5;Grade=2;Nodes=3/20;ER=Pos;PR=Pos;HER2=Neg;Age=68;DFS=156;Status=non-recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 518 labeled with Cy5


title: whole tissue section from case 633

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=633;Tumor.type=D;Size=2.7;Grade=3;Nodes=0/11;ER=Pos;PR=Pos;HER2=Neg;Age=61;DFS=20;Status=recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 633 labeled with Cy5


title: whole tissue section from case 374

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=374;Tumor.type=D;Size=1;Grade=3;Nodes=0/15;ER=Pos (<10%);PR=Pos;HER2=Pos;Age=57;DFS=47;Status=recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 374 labeled with Cy5


title: whole tissue section from case 648

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=648;Tumor.type=D;Size=1.1;Grade=2;Nodes=0/15;ER=Pos;PR=Pos;HER2=Neg;Age=62;DFS=160;Status=non-recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 648 labeled with Cy5


title: whole tissue section from case 317

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=317;Tumor.type=D;Size=1.2;Grade=3;Nodes=1/10;ER=Pos;PR=Neg;HER2=Neg;Age=71;DFS=5;Status=recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 317 labeled with Cy5


title: whole tissue section from case 319

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=319;Tumor.type=D;Size=4;Grade=2;Nodes=1/13;ER=Pos;PR=Pos;HER2=Neg;Age=67;DFS=44;Status=recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 319 labeled with Cy5


title: whole tissue section from case 260

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=260;Tumor.type=D/L;Size=0.9;Grade=2;Nodes=1/13;ER=Pos;PR=Pos;HER2=Neg;Age=73;DFS=42;Status=recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 260 labeled with Cy5


title: whole tissue section from case 297

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=297;Tumor.type=D;Size=0.8;Grade=2;Nodes=1/16;ER=Pos;PR=Pos;HER2=ND;Age=66;DFS=169;Status=non-recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 297 labeled with Cy5


title: whole tissue section from case 295

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=295;Tumor.type=D;Size=3.5;Grade=2;Nodes=3/21;ER=Pos;PR=Pos;HER2=Neg;Age=52;DFS=118;Status=recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 295 labeled with Cy5


title: whole tissue section from case 547

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=547;Tumor.type=D/L;Size=1.5;Grade=2;Nodes=ND;ER=Pos;PR=Pos;HER2=Neg;Age=74;DFS=129;Status=recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 547 labeled with Cy5

source_name: human universal reference RNA (Stratagene) labeled with Cy3


title: whole tissue section from case 289

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=289;Tumor.type=D;Size=3;Grade=2;Nodes=0/15;ER=Pos;PR=Pos;HER2=Neg;Age=75;DFS=63;Status=recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 289 labeled with Cy5


title: whole tissue section from case 180

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=180;Tumor.type=D;Size=1.6;Grade=2;Nodes=2/19;ER=Pos;PR=Pos;HER2=Neg ;Age=69;DFS=169;Status=non-recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 180 labeled with Cy5


title: whole tissue section from case 280

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=280;Tumor.type=D;Size=2.2;Grade=3;Nodes=0/12;ER=Pos;PR=Pos;HER2=Neg;Age=66;DFS=44;Status=recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 280 labeled with Cy5


title: whole tissue section from case 342

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=342;Tumor.type=D;Size=3;Grade=2;Nodes=9/21;ER=Pos;PR=Neg;HER2=Neg;Age=62;DFS=102;Status=recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 342 labeled with Cy5


title: Wild Type Control 3-NPA 1 Hour

city: Galveston

first: John

last: Papaconstantinou

address: 301 University, Mail Route 0643

department: Dept. of Human Biological Chemistry and Genetics

institute: University of Texas Medical Branch

description: RNA isolated by Chomczynski phenol/chloroform method from a 3-NPA treatment time course

source_name: Wild type liver


title: Snell Dwarf 3-NPA 0 Hour

source_name: Snell dwarf liver


title: Wild Type Control 3-NPA 20 Hour


title: Wild Type Control 3-NPA 42 Hour


title: Ts1Cje-5-MGU74A

status: Public on Jun 06 2004

city: Wako-shi

first: Kazuhiro

last: Yamakawa

state: Saitama

address: 2-1 Hirosawa

country: Japan

institute: RIKEN Brain Science Institute

description: Expression profile of genes in mouse brain

source_name: Brain


title: whole tissue section from case 59

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=59;Tumor.type=L;Size=3;Grade=2;Nodes=33/38;ER=Pos;PR=Pos;HER2=ID (+2);Age=70;DFS=21;Status=recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 59 labeled with Cy5


title: Snell Dwarf 3-NPA 1 Hour P.I.


title: Wild Type Control 3-NPA 15 minutes


title: Ts1Cje-6-MGU74A


title: whole tissue section from case 25

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=25;Tumor.type=D;Size=3.5;Grade=2;Nodes=0/9;ER=Neg;PR=Pos;HER2=Neg;Age=62;DFS=75;Status=recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 25 labeled with Cy5


title: Ts1Cje-2-MGU74A


title: Ts1Cje-1-MGU74A


title: Dog 3 at 24 weeks of HFD

city: Toulouse

first: Rouet

last: Philippe

state: FRANCE

address: 37 Allées Jules Guesde

institute: Louis Bugnard

description: mRNA from dog heart at 24 weeks of high fat diet

organism: Canis lupus familiaris

source_name: dog heart


title: Dog 2 at 24 weeks of HFD


title: 2N-6-MGU74A


title: 2N-5-MGU74A


title: Dog 1 at 24 weeks of HFD


title: Dog 4 at 24 weeks of HFD


title: Ts1Cje-3-MGU74A


title: Dog 3 at 15 weeks of HFD

description: mRNA from dog heart at 15 weeks of high fat diet


title: Ts1Cje-4-MGU74A


title: whole tissue section from case 68

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=68;Tumor.type=D;Size=3;Grade=2;Nodes=0/17;ER=Pos;PR=Pos;HER2=Neg;Age=53;DFS=38;Status=recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 68 labeled with Cy5


title: whole tissue section from case 655

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=655;Tumor.type=D;Size=2;Grade=3;Nodes=ND;ER=Pos;PR=Pos;HER2=ND;Age=73;DFS=143;Status=non-recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 655 labeled with Cy5


title: Dog 1 at 15 weeks of HFD


title: Dog 2 at 15 weeks of high fat diet


title: whole tissue section from case 140

description:
whole tissue section of breast cancer.
Clinical information: Sample.ID=140;Tumor.type=L;Size=>2.0;Grade=2;Nodes=18/28;ER=Pos;PR=Pos;HER2=Neg;Age=63;DFS=43;Status=recur
Keywords = breast cancer, tamoxifen, recurrence

source_name: RNA from whole tissue section of case 140 labeled with Cy5


title: 2N-4-MGU74A


title: Snell Dwarf 3-NPA 20 Hour P.I.


title: Snell Dwarf 3-NPA 15 minutes P.I.


title: 2N-1-MGU74A


title: Dog 5 at 10 weeks of HFD

description: mRNA from dog heart at 10 weeks of high fat diet


title: Wild Type Control 3-NPA 0 Hour


title: Dog 3 at 10 weeks of HFD


title: Dog 4 at 10 weeks of HFD


title: Ts1Cje-6-MGU74B


title: 2N-2-MGU74A


title: Dog 2 at 10 weeks of HFD


title: Ts1Cje-3-MGU74B


title: Ts1Cje-4-MGU74B


title: Ts1Cje-5-MGU74B


title: 2N-6-MGU74B


title: Ts1Cje-1-MGU74B


title: Control 3

description: mRNA from dog heart


title: Control 5

description: mRNA from dog heart


title: 2N-4-MGU74B


title: Dog 3 at 9 weeks of HFD

description: mRNA from dog heart at 9 weeks of high fat diet


title: Control 4

description: mRNA from dog heart


title: 2N-5-MGU74B


title: 2N-3-MGU74A


title: Dog 2 at 9 weeks HFD


title: Dog 1 at 9 weeks HFD

description: mRNA from dog heart at 9 weeks high fat diet


title: Ts1Cje-2-MGU74B


title: Dog 1 at 10 weeks of HFD


title: Control 2

description: mRNA from dog heart


title: 2N-3-MGU74B


title: Control 1

description: mRNA from dog heart


title: 2N-1-MGU74B


title: RAW264.7 12 hour LPS

status: Public on Jun 05 2004

city: Brisbane

first: Rachael

middle: Zoe

last: Murray

country: Australia

institute: IMB

description:
Total RNA was extracted from RAW264.7 cells using an Ambion RNaqueous RNA extraction kit. Targets were produced using modified Affymetrix procedure from 5ug of total RNA.
Keywords = RAW264.7 macrophage


title: 2N-2-MGU74B


title: RAW264.7 0 hour LPS


title: bacterial supernatant 1b

status: Public on Jun 03 2004

city: Sophia Antipolis

first: Chimene

last: Moreilhon

institute: CNRS

description: epithelial cells were in contact with S. aureus or not (control cells).These datas are the swapped datas of sample bacterial supernatant 1a

source_name: supernatant control 1

source_name: bacterial supernatant 1


title: HCT116-Clone2 4Gy 6hr (R4)

status: Public on Jun 04 2004

city: Ottawa

first: Felix

middle: M.

last: Mesak

state: ON

address: 503 Smyth Rd. 3rd Floor

department: Centre for Cancer Therapeutics

country: Canada

institute: Ottawa Regional Cancer Centre

description:
A cDNA microarray between X-radiation (XR) resistant cell clone HCT116-Clone2 and unirradiated control HCT116-Clone10 cells, a cell clone that has similar XR response with parental HCT116 cells. HCT116-Clone2 cells were treated with XR at dose 4 Gy. Their total mRNA was collected 6 hours after XR from different batches and pooled together. RNA extraction was done using RNeasy Mini kit (Qiagen Inc.). Total mRNA from HCT116-Clone2 or HCT116-Clone10 was labeled either with Cy5 or Cy3. This experiment was repeated four times with two of them was a reciprocal labeling. The arrays were scanned with ScanArray Express (Packard Bioscience). Acquired images were analyzed using QuantArray software version 3.0 (Packard Bioscience). The data was transformed with an Excel VBA macro Normaliser2 software (Institute for Biological Sciences, NRC, Ottawa, ON, Canada). Normaliser2 filtered the data by identifying problems with a spot on the array (flagging), which was then removed from the data set according to the set threshold i.e. spot intensity signal/sub array background less than 2.5, less than 5% spot intensity, and ignore spots with streak or damage.
Note:
FLAGS: IF, Ignore Filter; SB1 or 2, Substract Background CH1 or CH2; LI1 or 2, Low Intensity CH1 or CH2; HI1 or 2, High Intensity CH1 or CH2; NV, Nominal/Negative Value.
VALUE: Ratio (Log2)(Regression SubArray Normalised)(LogIntensity)(SubArray Bkgd Subtracted).

Keywords = X-radiation (XR)
Keywords = XR resistant
Keywords = colorectal cancer cells
Keywords = HCT116
Lot batch = ChipNo12646822_SSHuman19K4

source_name: Control HCT116-Clone10 cells (Cy3)

source_name: XR resistant HCT116-Clone2 cells (Cy5)


title: bacterial supernatant 2a

description: epithelial cells were in contact with S. aureus or not (control cells).These datas are the swapped datas of sample bacterial supernatant 2b

source_name: bacterial supernatant 2

source_name: supernatant control 2


title: bacterial supernatant 1a

description: epithelial cells were in contact with S. aureus or not (control cells).These datas are the swapped datas of sample bacterial supernatant 1b


title: HCT116-Clone2 4Gy 24hr (R4)

description:
A cDNA microarray between X-radiation (XR) resistant cell clone HCT116-Clone2 and unirradiated control HCT116-Clone10 cells, a cell clone that has similar XR response with parental HCT116 cells. HCT116-Clone2 cells were treated with XR at dose 4 Gy. Their total mRNA was collected 24 hours after XR from different batches and pooled together. RNA extraction was done using RNeasy Mini kit (Qiagen Inc.). Total mRNA from HCT116-Clone2 or HCT116-Clone10 was labeled either with Cy5 or Cy3. This experiment was repeated four times with two of them was a reciprocal labeling. The arrays were scanned with ScanArray Express (Packard Bioscience). Acquired images were analyzed using QuantArray software version 3.0 (Packard Bioscience). The data was transformed with an Excel VBA macro Normaliser2 software (Institute for Biological Sciences, NRC, Ottawa, ON, Canada). Normaliser2 filtered the data by identifying problems with a spot on the array (flagging), which was then removed from the data set according to the set threshold i.e. spot intensity signal/sub array background less than 2.5, less than 5% spot intensity, and ignore spots with streak or damage.
Note:
FLAGS: IF, Ignore Filter; SB1 or 2, Substract Background CH1 or CH2; LI1 or 2, Low Intensity CH1 or CH2; HI1 or 2, High Intensity CH1 or CH2; NV, Nominal/Negative Value.
VALUE: Ratio (Log2)(Regression SubArray Normalised)(LogIntensity)(SubArray Bkgd Subtracted).

Keywords = X-radiation (XR)
Keywords = XR resistant
Keywords = colorectal cancer cells
Keywords = HCT116
Lot batch = ChipNo12626422_SSHuman19K4

source_name: Control HCT116-Clone10 cells (Cy5)

source_name: XR resistant HCT116-Clone2 cells (Cy3)


title: bacterial supernatant 2b

description: epithelial cells were in contact with S. aureus or not (control cells).These datas are the swapped datas of sample bacterial supernatant 2a


title: bacterial supernatant 3a

description: epithelial cells were in contact with S. aureus or not (control cells).These datas are the swapped datas of sample bacterial supernatant 3b

source_name: bacterial supernatant 3

source_name: supernatant control 3


title: bacteria 3b

description: epithelial cells were in contact with S. aureus or not (control cells).These datas are the swapped datas of sample bacteria 3a

source_name: control 3

source_name: bacteria 3


title: bacteria 3a

description: epithelial cells were in contact with S. aureus or not (control cells).These datas are the swapped datas of sample bacteria 3b


title: HCT116-Clone2 4Gy 6hr (R2)

description:
A cDNA microarray between X-radiation (XR) resistant cell clone HCT116-Clone2 and unirradiated control HCT116-Clone10 cells, a cell clone that has similar XR response with parental HCT116 cells. HCT116-Clone2 cells were treated with XR at dose 4 Gy. Their total mRNA was collected 6 hours after XR from different batches and pooled together. RNA extraction was done using RNeasy Mini kit (Qiagen Inc.). Total mRNA from HCT116-Clone2 or HCT116-Clone10 was labeled either with Cy5 or Cy3. This experiment was repeated four times with two of them was a reciprocal labeling. The arrays were scanned with ScanArray Express (Packard Bioscience). Acquired images were analyzed using QuantArray software version 3.0 (Packard Bioscience). The data was transformed with an Excel VBA macro Normaliser2 software (Institute for Biological Sciences, NRC, Ottawa, ON, Canada). Normaliser2 filtered the data by identifying problems with a spot on the array (flagging), which was then removed from the data set according to the set threshold i.e. spot intensity signal/sub array background less than 2.5, less than 5% spot intensity, and ignore spots with streak or damage.
Note:
FLAGS: IF, Ignore Filter; SB1 or 2, Substract Background CH1 or CH2; LI1 or 2, Low Intensity CH1 or CH2; HI1 or 2, High Intensity CH1 or CH2; NV, Nominal/Negative Value.
VALUE: Ratio (Log2)(Regression SubArray Normalised)(LogIntensity)(SubArray Bkgd Subtracted).

Keywords = X-radiation (XR)
Keywords = XR resistant
Keywords = colorectal cancer cells
Keywords = HCT116
Lot batch = ChipNo12626660_SSHuman19K4


title: HCT116-Clone2 4Gy 10min (R1)

description:
A cDNA microarray between X-radiation (XR) resistant cell clone HCT116-Clone2 and unirradiated control HCT116-Clone10 cells, a cell clone that has similar XR response with parental HCT116 cells. HCT116-Clone2 cells were treated with XR at dose 4 Gy. Their total mRNA was collected 10 minutes after XR from different batches and pooled together. RNA extraction was done using RNeasy Mini kit (Qiagen Inc.). Total mRNA from HCT116-Clone2 or HCT116-Clone10 was labeled either with Cy5 or Cy3. This experiment was repeated four times with two of them was a reciprocal labeling. The arrays were scanned with ScanArray Express (Packard Bioscience). Acquired images were analyzed using QuantArray software version 3.0 (Packard Bioscience). The data was transformed with an Excel VBA macro Normaliser2 software (Institute for Biological Sciences, NRC, Ottawa, ON, Canada). Normaliser2 filtered the data by identifying problems with a spot on the array (flagging), which was then removed from the data set according to the set threshold i.e. spot intensity signal/sub array background less than 2.5, less than 5% spot intensity, and ignore spots with streak or damage.
Note:
FLAGS: IF, Ignore Filter; SB1 or 2, Substract Background CH1 or CH2; LI1 or 2, Low Intensity CH1 or CH2; HI1 or 2, High Intensity CH1 or CH2; NV, Nominal/Negative Value.
VALUE: Ratio (Log2)(Regression SubArray Normalised)(LogIntensity)(SubArray Bkgd Subtracted).

Keywords = X-radiation (XR)
Keywords = XR resistant
Keywords = colorectal cancer cells
Keywords = HCT116
Lot batch = ChipNo12643680_SSHuman19K4


title: HCT116-Clone2 4Gy 24hr (R3)

description:
A cDNA microarray between X-radiation (XR) resistant cell clone HCT116-Clone2 and unirradiated control HCT116-Clone10 cells, a cell clone that has similar XR response with parental HCT116 cells. HCT116-Clone2 cells were treated with XR at dose 4 Gy. Their total mRNA was collected 24 hours after XR from different batches and pooled together. RNA extraction was done using RNeasy Mini kit (Qiagen Inc.). Total mRNA from HCT116-Clone2 or HCT116-Clone10 was labeled either with Cy5 or Cy3. This experiment was repeated four times with two of them was a reciprocal labeling. The arrays were scanned with ScanArray Express (Packard Bioscience). Acquired images were analyzed using QuantArray software version 3.0 (Packard Bioscience). The data was transformed with an Excel VBA macro Normaliser2 software (Institute for Biological Sciences, NRC, Ottawa, ON, Canada). Normaliser2 filtered the data by identifying problems with a spot on the array (flagging), which was then removed from the data set according to the set threshold i.e. spot intensity signal/sub array background less than 2.5, less than 5% spot intensity, and ignore spots with streak or damage.
Note:
FLAGS: IF, Ignore Filter; SB1 or 2, Substract Background CH1 or CH2; LI1 or 2, Low Intensity CH1 or CH2; HI1 or 2, High Intensity CH1 or CH2; NV, Nominal/Negative Value.
VALUE: Ratio (Log2)(Regression SubArray Normalised)(LogIntensity)(SubArray Bkgd Subtracted).

Keywords = X-radiation (XR)
Keywords = XR resistant
Keywords = colorectal cancer cells
Keywords = HCT116
Lot batch = ChipNo12626034_SSHuman19K4


title: HCT116-Clone2 4Gy 6hr (R3)

description:
A cDNA microarray between X-radiation (XR) resistant cell clone HCT116-Clone2 and unirradiated control HCT116-Clone10 cells, a cell clone that has similar XR response with parental HCT116 cells. HCT116-Clone2 cells were treated with XR at dose 4 Gy. Their total mRNA was collected 6 hours after XR from different batches and pooled together. RNA extraction was done using RNeasy Mini kit (Qiagen Inc.). Total mRNA from HCT116-Clone2 or HCT116-Clone10 was labeled either with Cy5 or Cy3. This experiment was repeated four times with two of them was a reciprocal labeling. The arrays were scanned with ScanArray Express (Packard Bioscience). Acquired images were analyzed using QuantArray software version 3.0 (Packard Bioscience). The data was transformed with an Excel VBA macro Normaliser2 software (Institute for Biological Sciences, NRC, Ottawa, ON, Canada). Normaliser2 filtered the data by identifying problems with a spot on the array (flagging), which was then removed from the data set according to the set threshold i.e. spot intensity signal/sub array background less than 2.5, less than 5% spot intensity, and ignore spots with streak or damage.
Note:
FLAGS: IF, Ignore Filter; SB1 or 2, Substract Background CH1 or CH2; LI1 or 2, Low Intensity CH1 or CH2; HI1 or 2, High Intensity CH1 or CH2; NV, Nominal/Negative Value.
VALUE: Ratio (Log2)(Regression SubArray Normalised)(LogIntensity)(SubArray Bkgd Subtracted).

Keywords = X-radiation (XR)
Keywords = XR resistant
Keywords = colorectal cancer cells
Keywords = HCT116
Lot batch = ChipNo12643418_SSHuman19K4


title: HCT116-Clone2 4Gy 24hr (R1)

description:
A cDNA microarray between X-radiation (XR) resistant cell clone HCT116-Clone2 and unirradiated control HCT116-Clone10 cells, a cell clone that has similar XR response with parental HCT116 cells. HCT116-Clone2 cells were treated with XR at dose 4 Gy. Their total mRNA was collected 24 hours after XR from different batches and pooled together. RNA extraction was done using RNeasy Mini kit (Qiagen Inc.). Total mRNA from HCT116-Clone2 or HCT116-Clone10 was labeled either with Cy5 or Cy3. This experiment was repeated four times with two of them was a reciprocal labeling. The arrays were scanned with ScanArray Express (Packard Bioscience). Acquired images were analyzed using QuantArray software version 3.0 (Packard Bioscience). The data was transformed with an Excel VBA macro Normaliser2 software (Institute for Biological Sciences, NRC, Ottawa, ON, Canada). Normaliser2 filtered the data by identifying problems with a spot on the array (flagging), which was then removed from the data set according to the set threshold i.e. spot intensity signal/sub array background less than 2.5, less than 5% spot intensity, and ignore spots with streak or damage.
Note:
FLAGS: IF, Ignore Filter; SB1 or 2, Substract Background CH1 or CH2; LI1 or 2, Low Intensity CH1 or CH2; HI1 or 2, High Intensity CH1 or CH2; NV, Nominal/Negative Value.
VALUE: Ratio (Log2)(Regression SubArray Normalised)(LogIntensity)(SubArray Bkgd Subtracted).

Keywords = X-radiation (XR)
Keywords = XR resistant
Keywords = colorectal cancer cells
Keywords = HCT116
Lot batch = ChipNo12626028_SSHuman19K4


title: Unirradiated HCT116-Clone2 (R2)

description:
A cDNA microarray between unirradiated X-radiation (XR) resistant cell clone HCT116-Clone2 and unirradiated control HCT116-Clone10 cells, a cell clone which has similar XR response with parental HCT116 cells. Their total mRNA was collected from different batches and pooled together. RNA extraction was done using RNeasy Mini kit (Qiagen Inc.). Total mRNA from HCT116-Clone2 or HCT116-Clone10 was labeled either with Cy5 or Cy3. This experiment was repeated four times with two of them was a reciprocal labeling. The arrays were scanned with ScanArray Express (Packard Bioscience). Acquired images were analyzed using QuantArray software version 3.0 (Packard Bioscience). The data was transformed with an Excel VBA macro Normaliser2 software (Institute for Biological Sciences, NRC, Ottawa, ON, Canada). Normaliser2 filtered the data by identifying problems with a spot on the array (flagging), which was then removed from the data set according to the set threshold i.e. spot intensity signal/sub array background less than 2.5, less than 5% spot intensity, and ignore spots with streak or damage.
Note:
FLAGS: IF, Ignore Filter; SB1 or 2, Substract Background CH1 or CH2; LI1 or 2, Low Intensity CH1 or CH2; HI1 or 2, High Intensity CH1 or CH2; NV, Nominal/Negative Value.
VALUE: Ratio (Log2)(Regression SubArray Normalised)(LogIntensity)(SubArray Bkgd Subtracted).

Keywords = X-radiation (XR)
Keywords = XR resistant
Keywords = colorectal cancer cells
Keywords = HCT116
Lot batch = ChipNo12626421_SSHuman19K4


title: HCT116-Clone2 4Gy 6hr (R1)

description:
A cDNA microarray between X-radiation (XR) resistant cell clone HCT116-Clone2 and unirradiated control HCT116-Clone10 cells, a cell clone that has similar XR response with parental HCT116 cells. HCT116-Clone2 cells were treated with XR at dose 4 Gy. Their total mRNA was collected 6 hours after XR from different batches and pooled together. RNA extraction was done using RNeasy Mini kit (Qiagen Inc.). Total mRNA from HCT116-Clone2 or HCT116-Clone10 was labeled either with Cy5 or Cy3. This experiment was repeated four times with two of them was a reciprocal labeling. The arrays were scanned with ScanArray Express (Packard Bioscience). Acquired images were analyzed using QuantArray software version 3.0 (Packard Bioscience). The data was transformed with an Excel VBA macro Normaliser2 software (Institute for Biological Sciences, NRC, Ottawa, ON, Canada). Normaliser2 filtered the data by identifying problems with a spot on the array (flagging), which was then removed from the data set according to the set threshold i.e. spot intensity signal/sub array background less than 2.5, less than 5% spot intensity, and ignore spots with streak or damage.
Note:
FLAGS: IF, Ignore Filter; SB1 or 2, Substract Background CH1 or CH2; LI1 or 2, Low Intensity CH1 or CH2; HI1 or 2, High Intensity CH1 or CH2; NV, Nominal/Negative Value.
VALUE: Ratio (Log2)(Regression SubArray Normalised)(LogIntensity)(SubArray Bkgd Subtracted).

Keywords = X-radiation (XR)
Keywords = XR resistant
Keywords = colorectal cancer cells
Keywords = HCT116
Lot batch = ChipNo12626030_SSHuman19K4


title: HCT116-Clone2 4Gy 10min (R4)

description:
A cDNA microarray between X-radiation (XR) resistant cell clone HCT116-Clone2 and unirradiated control HCT116-Clone10 cells, a cell clone that has similar XR response with parental HCT116 cells. HCT116-Clone2 cells were treated with XR at dose 4 Gy. Their total mRNA was collected 10 minutes after XR from different batches and pooled together. RNA extraction was done using RNeasy Mini kit (Qiagen Inc.). Total mRNA from HCT116-Clone2 or HCT116-Clone10 was labeled either with Cy5 or Cy3. This experiment was repeated four times with two of them was a reciprocal labeling. The arrays were scanned with ScanArray Express (Packard Bioscience). Acquired images were analyzed using QuantArray software version 3.0 (Packard Bioscience). The data was transformed with an Excel VBA macro Normaliser2 software (Institute for Biological Sciences, NRC, Ottawa, ON, Canada). Normaliser2 filtered the data by identifying problems with a spot on the array (flagging), which was then removed from the data set according to the set threshold i.e. spot intensity signal/sub array background less than 2.5, less than 5% spot intensity, and ignore spots with streak or damage.
Note:
FLAGS: IF, Ignore Filter; SB1 or 2, Substract Background CH1 or CH2; LI1 or 2, Low Intensity CH1 or CH2; HI1 or 2, High Intensity CH1 or CH2; NV, Nominal/Negative Value.
VALUE: Ratio (Log2)(Regression SubArray Normalised)(LogIntensity)(SubArray Bkgd Subtracted).

Keywords = X-radiation (XR)
Keywords = XR resistant
Keywords = colorectal cancer cells
Keywords = HCT116
Lot batch = ChipNo12643683_SSHuman19K4


title: HCT116-Clone2 4Gy 24hr (R2)

description:
A cDNA microarray between X-radiation (XR) resistant cell clone HCT116-Clone2 and unirradiated control HCT116-Clone10 cells, a cell clone that has similar XR response with parental HCT116 cells. HCT116-Clone2 cells were treated with XR at dose 4 Gy. Their total mRNA was collected 24 hours after XR from different batches and pooled together. RNA extraction was done using RNeasy Mini kit (Qiagen Inc.). Total mRNA from HCT116-Clone2 or HCT116-Clone10 was labeled either with Cy5 or Cy3. This experiment was repeated four times with two of them was a reciprocal labeling. The arrays were scanned with ScanArray Express (Packard Bioscience). Acquired images were analyzed using QuantArray software version 3.0 (Packard Bioscience). The data was transformed with an Excel VBA macro Normaliser2 software (Institute for Biological Sciences, NRC, Ottawa, ON, Canada). Normaliser2 filtered the data by identifying problems with a spot on the array (flagging), which was then removed from the data set according to the set threshold i.e. spot intensity signal/sub array background less than 2.5, less than 5% spot intensity, and ignore spots with streak or damage.
Note:
FLAGS: IF, Ignore Filter; SB1 or 2, Substract Background CH1 or CH2; LI1 or 2, Low Intensity CH1 or CH2; HI1 or 2, High Intensity CH1 or CH2; NV, Nominal/Negative Value.
VALUE: Ratio (Log2)(Regression SubArray Normalised)(LogIntensity)(SubArray Bkgd Subtracted).

Keywords = X-radiation (XR)
Keywords = XR resistant
Keywords = colorectal cancer cells
Keywords = HCT116
Lot batch = ChipNo12626029_SSHuman19K4


title: Unirradiated HCT116-Clone2 (R3)

description:
A cDNA microarray between unirradiated X-radiation (XR) resistant cell clone HCT116-Clone2 and unirradiated control HCT116-Clone10 cells, a cell clone which has similar XR response with parental HCT116 cells. Their total mRNA was collected from different batches and pooled together. RNA extraction was done using RNeasy Mini kit (Qiagen Inc.). Total mRNA from HCT116-Clone2 or HCT116-Clone10 was labeled either with Cy5 or Cy3. This experiment was repeated four times with two of them was a reciprocal labeling. The arrays were scanned with ScanArray Express (Packard Bioscience). Acquired images were analyzed using QuantArray software version 3.0 (Packard Bioscience). The data was transformed with an Excel VBA macro Normaliser2 software (Institute for Biological Sciences, NRC, Ottawa, ON, Canada). Normaliser2 filtered the data by identifying problems with a spot on the array (flagging), which was then removed from the data set according to the set threshold i.e. spot intensity signal/sub array background less than 2.5, less than 5% spot intensity, and ignore spots with streak or damage.
Note:
FLAGS: IF, Ignore Filter; SB1 or 2, Substract Background CH1 or CH2; LI1 or 2, Low Intensity CH1 or CH2; HI1 or 2, High Intensity CH1 or CH2; NV, Nominal/Negative Value.
VALUE: Ratio (Log2)(Regression SubArray Normalised)(LogIntensity)(SubArray Bkgd Subtracted).

Keywords = X-radiation (XR)
Keywords = XR resistant
Keywords = colorectal cancer cells
Keywords = HCT116
Lot batch = ChipNo12626426_SSHuman19K4


title: HCT116-Clone2 4Gy 10min (R3)

description:
A cDNA microarray between X-radiation (XR) resistant cell clone HCT116-Clone2 and unirradiated control HCT116-Clone10 cells, a cell clone that has similar XR response with parental HCT116 cells. HCT116-Clone2 cells were treated with XR at dose 4 Gy. Their total mRNA was collected 10 minutes after XR from different batches and pooled together. RNA extraction was done using RNeasy Mini kit (Qiagen Inc.). Total mRNA from HCT116-Clone2 or HCT116-Clone10 was labeled either with Cy5 or Cy3. This experiment was repeated four times with two of them was a reciprocal labeling. The arrays were scanned with ScanArray Express (Packard Bioscience). Acquired images were analyzed using QuantArray software version 3.0 (Packard Bioscience). The data was transformed with an Excel VBA macro Normaliser2 software (Institute for Biological Sciences, NRC, Ottawa, ON, Canada). Normaliser2 filtered the data by identifying problems with a spot on the array (flagging), which was then removed from the data set according to the set threshold i.e. spot intensity signal/sub array background less than 2.5, less than 5% spot intensity, and ignore spots with streak or damage.
Note:
FLAGS: IF, Ignore Filter; SB1 or 2, Substract Background CH1 or CH2; LI1 or 2, Low Intensity CH1 or CH2; HI1 or 2, High Intensity CH1 or CH2; NV, Nominal/Negative Value.
VALUE: Ratio (Log2)(Regression SubArray Normalised)(LogIntensity)(SubArray Bkgd Subtracted).

Keywords = X-radiation (XR)
Keywords = XR resistant
Keywords = colorectal cancer cells
Keywords = HCT116
Lot batch = ChipNo12643682_SSHuman19K4


title: HCT116-Clone2 4Gy 10min (R2)

description:
A cDNA microarray between X-radiation (XR) resistant cell clone HCT116-Clone2 and unirradiated control HCT116-Clone10 cells, a cell clone that has similar XR response with parental HCT116 cells. HCT116-Clone2 cells were treated with XR at dose 4 Gy. Their total mRNA was collected 10 minutes after XR from different batches and pooled together. RNA extraction was done using RNeasy Mini kit (Qiagen Inc.). Total mRNA from HCT116-Clone2 or HCT116-Clone10 was labeled either with Cy5 or Cy3. This experiment was repeated four times with two of them was a reciprocal labeling. The arrays were scanned with ScanArray Express (Packard Bioscience). Acquired images were analyzed using QuantArray software version 3.0 (Packard Bioscience). The data was transformed with an Excel VBA macro Normaliser2 software (Institute for Biological Sciences, NRC, Ottawa, ON, Canada). Normaliser2 filtered the data by identifying problems with a spot on the array (flagging), which was then removed from the data set according to the set threshold i.e. spot intensity signal/sub array background less than 2.5, less than 5% spot intensity, and ignore spots with streak or damage.
Note:
FLAGS: IF, Ignore Filter; SB1 or 2, Substract Background CH1 or CH2; LI1 or 2, Low Intensity CH1 or CH2; HI1 or 2, High Intensity CH1 or CH2; NV, Nominal/Negative Value.
VALUE: Ratio (Log2)(Regression SubArray Normalised)(LogIntensity)(SubArray Bkgd Subtracted).

Keywords = X-radiation (XR)
Keywords = XR resistant
Keywords = colorectal cancer cells
Keywords = HCT116
Lot batch = ChipNo12643681_SSHuman19K4


title: bacterial supernatant 3b

description: epithelial cells were in contact with S. aureus or not (control cells).These datas are the swapped datas of sample bacterial supernatant 3a


title: RAW264.7 2 hour LPS

description:
Total RNA was extracted from RAW264.7 cells using an Ambion RNaqueous RNA extraction kit. Targets were produced using modified Affymetrix procedure from 5ug of total RNA.
Keywords = RAW264.7 macrophage


title: bacteria 2b

description: epithelial cells were in contact with S. aureus or not (control cells).These datas are the swapped datas of sample bacteria 2a

source_name: control 2

source_name: bacteria 2


title: Unirradiated HCT116-Clone2 (R4)

description:
A cDNA microarray between unirradiated X-radiation (XR) resistant cell clone HCT116-Clone2 and unirradiated control HCT116-Clone10 cells, a cell clone which has similar XR response with parental HCT116 cells. Their total mRNA was collected from different batches and pooled together. RNA extraction was done using RNeasy Mini kit (Qiagen Inc.). Total mRNA from HCT116-Clone2 or HCT116-Clone10 was labeled either with Cy5 or Cy3. This experiment was repeated four times with two of them was a reciprocal labeling. The arrays were scanned with ScanArray Express (Packard Bioscience). Acquired images were analyzed using QuantArray software version 3.0 (Packard Bioscience). The data was transformed with an Excel VBA macro Normaliser2 software (Institute for Biological Sciences, NRC, Ottawa, ON, Canada). Normaliser2 filtered the data by identifying problems with a spot on the array (flagging), which was then removed from the data set according to the set threshold i.e. spot intensity signal/sub array background less than 2.5, less than 5% spot intensity, and ignore spots with streak or damage.
Note:
FLAGS: IF, Ignore Filter; SB1 or 2, Substract Background CH1 or CH2; LI1 or 2, Low Intensity CH1 or CH2; HI1 or 2, High Intensity CH1 or CH2; NV, Nominal/Negative Value.
VALUE: Ratio (Log2)(Regression SubArray Normalised)(LogIntensity)(SubArray Bkgd Subtracted).

Keywords = X-radiation (XR)
Keywords = XR resistant
Keywords = colorectal cancer cells
Keywords = HCT116
Lot batch = ChipNo12626427_SSHuman19K4


title: Unirradiated HCT116-Clone2

description:
A cDNA microarray between unirradiated X-radiation (XR) resistant cell clone HCT116-Clone2 and unirradiated control HCT116-Clone10 cells, a cell clone which has similar XR response with parental HCT116 cells. Their total mRNA was collected from different batches and pooled together. RNA extraction was done using RNeasy Mini kit (Qiagen Inc.). Total mRNA from HCT116-Clone2 or HCT116-Clone10 was labeled either with Cy5 or Cy3. This experiment was repeated four times with two of them was a reciprocal labeling. The arrays were scanned with ScanArray Express (Packard Bioscience). Acquired images were analyzed using QuantArray software version 3.0 (Packard Bioscience). The data was transformed with an Excel VBA macro Normaliser2 software (Institute for Biological Sciences, NRC, Ottawa, ON, Canada). Normaliser2 filtered the data by identifying problems with a spot on the array (flagging), which was then removed from the data set according to the set threshold i.e. spot intensity signal/sub array background less than 2.5, less than 5% spot intensity, and ignore spots with streak or damage.
Note:
FLAGS: IF, Ignore Filter; SB1 or 2, Substract Background CH1 or CH2; LI1 or 2, Low Intensity CH1 or CH2; HI1 or 2, High Intensity CH1 or CH2; NV, Nominal/Negative Value.
VALUE: Ratio (Log2)(Regression SubArray Normalised)(LogIntensity)(SubArray Bkgd Subtracted).

Keywords = X-radiation (XR)
Keywords = XR resistant
Keywords = colorectal cancer cells
Keywords = HCT116
Lot batch = ChipNo12626420_SSHuman19K4


title: bacteria 2a

description:
epithelial cells were in contact with S. aureus or not (control cells).
These datas are the swapped datas of sample bacteria 2b


title: P-12h-9-C

status: Public on Jun 02 2004

city: Baltimore

first: Myriam

last: Gorospe

state: MD

address: 5600 Nathan Shock Drive

institute: NIA

description: RNA from polysomal fraction #9 from cells collected 12h after UVC treatment. Biological repeat 3

source_name: RKO cells


title: P-12h-8-C

description: RNA from polysomal fraction #8 from cells collected 12h after UVC treatment. Biological repeat 3


title: bacteria 1b

description:
epithelial cells were in contact with S. aureus or not (control cells).
These datas are the swapped datas of sample bacteria 1a

source_name: bacteria 1


title: P-12h-11-C

description: RNA from polysomal fraction #11 from cells collected 12h after UVC treatment. Biological repeat 3


title: P-12h-6-C

description: RNA from polysomal fraction #6 from cells collected 12h after UVC treatment. Biological repeat 3


title: P-12h-3-C

description: RNA from polysomal fraction #3 from cells collected 12h after UVC treatment. Biological repeat 3


title: P-12h-7-C

description: RNA from polysomal fraction #7 from cells collected 12h after UVC treatment. Biological repeat 3


title: bacteria 1a

description:
epithelial cells were in contact with S. aureus or not (control cells).
These datas are the swapped datas of sample bacteria 1b

source_name: control 1


title: P-12h-1-C

description: RNA from polysomal fraction #1 from cells collected 12h after UVC treatment. Biological repeat 3


title: P-12h-2-C

description: RNA from polysomal fraction #2 from cells collected 12h after UVC treatment. Biological repeat 3


title: P-12h-11-B

description: RNA from polysomal fraction #11 from cells collected 12h after UVC treatment. Biological repeat 2


title: P-12h-6-B

description: RNA from polysomal fraction #6 from cells collected 12h after UVC treatment. Biological repeat 2


title: P-12h-7-B

description: RNA from polysomal fraction #7 from cells collected 12h after UVC treatment. Biological repeat 2


title: P-12h-11-A

description: RNA from polysomal fraction #11 from cells collected 12h after UVC treatment. Biological repeat 1


title: P-12h-3-B

description: RNA from polysomal fraction #3 from cells collected 12h after UVC treatment. Biological repeat 2


title: P-12h-5-B

description: RNA from polysomal fraction #5 from cells collected 12h after UVC treatment. Biological repeat 2


title: P-12h-8-B

description: RNA from polysomal fraction #8 from cells collected 12h after UVC treatment. Biological repeat 2


title: P-12h-1-B

description: RNA from polysomal fraction #1 from cells collected 12h after UVC treatment. Biological repeat 2


title: P-12h-10-B

description: RNA from polysomal fraction #10 from cells collected 12h after UVC treatment. Biological repeat 2


title: P-12h-2-B

description: RNA from polysomal fraction #2 from cells collected 12h after UVC treatment. Biological repeat 2


title: P-12h-9-B

description: RNA from polysomal fraction #9 from cells collected 12h after UVC treatment. Biological repeat 2


title: P-12h-10-A

description: RNA from polysomal fraction #10 from cells collected 12h after UVC treatment. Biological repeat 1


title: P-12h-4-B

description: RNA from polysomal fraction #4 from cells collected 12h after UVC treatment. Biological repeat 2


title: P-12h-9-A

description: RNA from polysomal fraction #9 from cells collected 12h after UVC treatment. Biological repeat 1


title: P-12h-7-A

description: RNA from polysomal fraction #7 from cells collected 12h after UVC treatment. Biological repeat 1


title: P-12h-3-A

description: RNA from polysomal fraction #3 from cells collected 12h after UVC treatment. Biological repeat 1


title: P-12h-6-A

description: RNA from polysomal fraction #6 from cells collected 12h after UVC treatment. Biological repeat 1


title: P-12h-8-A

description: RNA from polysomal fraction #8 from cells collected 12h after UVC treatment. Biological repeat 1


title: P-12h-4-A

description: RNA from polysomal fraction #4 from cells collected 12h after UVC treatment. Biological repeat 1


title: P-12h-5-A

description: RNA from polysomal fraction #5 from cells collected 12h after UVC treatment. Biological repeat 1


title: P-12h-1-A

description: RNA from polysomal fraction #1 from cells collected 12h after UVC treatment. Biological repeat 1


title: P-12h-2-A

description: RNA from polysomal fraction #2 from cells collected 12h after UVC treatment. Biological repeat 1


title: P-6h-9-C

description: RNA from polysomal fraction #9 from cells collected 6h after UVC treatment. Biological repeat 3


title: P-6h-2-C

description: RNA from polysomal fraction #2 from cells collected 6h after UVC treatment. Biological repeat 3


title: P-6h-3-C

description: RNA from polysomal fraction #3 from cells collected 6h after UVC treatment. Biological repeat 3


title: P-6h-10-C

description: RNA from polysomal fraction #10 from cells collected 6h after UVC treatment. Biological repeat 3


title: P-6h-6-C

description: RNA from polysomal fraction #6 from cells collected 6h after UVC treatment. Biological repeat 3


title: P-6h-10-B

description: RNA from polysomal fraction #10 from cells collected 6h after UVC treatment. Biological repeat 2


title: P-6h-11-B

description: RNA from polysomal fraction #11 from cells collected 6h after UVC treatment. Biological repeat 2


title: P-6h-7-C

description: RNA from polysomal fraction #7 from cells collected 6h after UVC treatment. Biological repeat 3


title: P-6h-8-C

description: RNA from polysomal fraction #8 from cells collected 6h after UVC treatment. Biological repeat 3


title: P-6h-9-B

description: RNA from polysomal fraction #9 from cells collected 6h after UVC treatment. Biological repeat 2


title: P-6h-4-C

description: RNA from polysomal fraction #4 from cells collected 6h after UVC treatment. Biological repeat 3


title: P-6h-6-B

description: RNA from polysomal fraction #6 from cells collected 6h after UVC treatment. Biological repeat 2


title: P-6h-8-B

description: RNA from polysomal fraction #8 from cells collected 6h after UVC treatment. Biological repeat 2


title: P-6h-5-C

description: RNA from polysomal fraction #5 from cells collected 6h after UVC treatment. Biological repeat 3


title: P-6h-11-C

description: RNA from polysomal fraction #11 from cells collected 6h after UVC treatment. Biological repeat 3


title: P-6h-1-C

description: RNA from polysomal fraction #1 from cells collected 6h after UVC treatment. Biological repeat 3


title: P-6h-7-B

description: RNA from polysomal fraction #7 from cells collected 6h after UVC treatment. Biological repeat 2


title: P-6h-4-B

description: RNA from polysomal fraction #4 from cells collected 6h after UVC treatment. Biological repeat 2


title: P-6h-5-B

description: RNA from polysomal fraction #5 from cells collected 6h after UVC treatment. Biological repeat 2


title: P-6h-3-B

description: RNA from polysomal fraction #3 from cells collected 6h after UVC treatment. Biological repeat 2


title: P-6h-6-A

description: RNA from polysomal fraction #6 from cells collected 6h after UVC treatment. Biological repeat 1


title: P-6h-7-A

description: RNA from polysomal fraction #7 from cells collected 6h after UVC treatment. Biological repeat 1


title: P-6h-9-A

description: RNA from polysomal fraction #9 from cells collected 6h after UVC treatment. Biological repeat 1


title: P-6h-8-A

description: RNA from polysomal fraction #8 from cells collected 6h after UVC treatment. Biological repeat 1


title: P-6h-10-A

description: RNA from polysomal fraction #10 from cells collected 6h after UVC treatment. Biological repeat 1


title: P-3h-10-C

description: RNA from polysomal fraction #10 from cells collected 3h after UVC treatment. Biological repeat 3


title: P-3h-1-C

description: RNA from polysomal fraction #1 from cells collected 3h after UVC treatment. Biological repeat 3


title: P-6h-1-B

description: RNA from polysomal fraction #1 from cells collected 6h after UVC treatment. Biological repeat 2


title: P-6h-2-B

description: RNA from polysomal fraction #2 from cells collected 6h after UVC treatment. Biological repeat 2


title: P-6h-2-A

description: RNA from polysomal fraction #2 from cells collected 6h after UVC treatment. Biological repeat 1


title: P-6h-3-A

description: RNA from polysomal fraction #3 from cells collected 6h after UVC treatment. Biological repeat 1


title: P-3h-11-C

description: RNA from polysomal fraction #11 from cells collected 3h after UVC treatment. Biological repeat 3


title: P-6h-4-A

description: RNA from polysomal fraction #4 from cells collected 6h after UVC treatment. Biological repeat 1


title: P-6h-1-A

description: RNA from polysomal fraction #1 from cells collected 6h after UVC treatment. Biological repeat 1


title: P-6h-11-A

description: RNA from polysomal fraction #11 from cells collected 6h after UVC treatment. Biological repeat 1


title: P-3h-4-C

description: RNA from polysomal fraction #4 from cells collected 3h after UVC treatment. Biological repeat 3


title: P-3h-2-C

description: RNA from polysomal fraction #2 from cells collected 3h after UVC treatment. Biological repeat 3


title: P-6h-5-A

description: RNA from polysomal fraction #5 from cells collected 6h after UVC treatment. Biological repeat 1


title: P-3h-9-C

description: RNA from polysomal fraction #9 from cells collected 3h after UVC treatment. Biological repeat 3


title: P-3h-6-C

description: RNA from polysomal fraction #6 from cells collected 3h after UVC treatment. Biological repeat 3


title: P-3h-8-C

description: RNA from polysomal fraction #8 from cells collected 3h after UVC treatment. Biological repeat 3


title: P-3h-7-C

description: RNA from polysomal fraction #7 from cells collected 3h after UVC treatment. Biological repeat 3


title: P-3h-3-C

description: RNA from polysomal fraction #3 from cells collected 3h after UVC treatment. Biological repeat 3


title: P-3h-10-B

description: RNA from polysomal fraction #10 from cells collected 3h after UVC treatment. Biological repeat 2