title: 11552H

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 58
Sample_probe_id: 448


title: 15208M

status: Public on Oct 29 2003

type: RNA

city: South San Francisco

first: Scott

middle: Curtis

last: Lohr

state: CA

address: 290 Utah Ave.

department: Bioinformatics

country: USA

institute: AGY Therapeutics Inc.

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 50
Sample_probe_id: 450

molecule: total RNA

organism: Homo sapiens

source_name: BV2, Abeta, 24h, stimulated


title: 15208F

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 69
Sample_probe_id: 447

source_name: BV2, Abeta, 24h, control


title: 15208D2

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 39
Sample_probe_id: 469


title: 15208C2

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 33
Sample_probe_id: 469


title: 11548K

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 61
Sample_probe_id: 449


title: 11548L

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 80
Sample_probe_id: 449


title: 11552D

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 79
Sample_probe_id: 447


title: 11552E

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 85
Sample_probe_id: 447


title: 11548J

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 88
Sample_probe_id: 449


title: 07886L

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 107
Sample_probe_id: 449


title: 15208B2

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 40
Sample_probe_id: 469


title: 07886K

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 105
Sample_probe_id: 449


title: 11552D2

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 20
Sample_probe_id: 469


title: 11552C2

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 66
Sample_probe_id: 469


title: 07886J

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 104
Sample_probe_id: 449


title: 11552J


title: 11548O

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 221
Sample_probe_id: 450


title: 11548N

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 222
Sample_probe_id: 450


title: SC 1-RC-2

status: Public on Oct 30 2003

city: La Jolla

first: Jun

last: Lim

address: 10010 N. Torrey Pines Rd.

department: PBIO-E

institute: The Salk Institute for Biological Studies

description:
cRNA from Arabidopsis suspension cell culture
Keywords = Arabidopsis suspension cell culture, tiling arrays

organism: Arabidopsis thaliana

source_name: Arabidopsis suspension cell culture


title: whole-genome 1-rc-2 L+

city: Albany

first: Atsunari

last: Tsuchisaka

address: 800 Buchanan St.

department: Plant Gene Expression Center

institute: University of California, Berkeley / USDA

description: Total RNA was isolated from seedlings with the TRIzol reagent procedure of Invitrogen, then poly(A)+ RNA was purified with Qiagen oligotex. One microgram poly(A)+ RNA was converted into ds-cDNA using T7-oligo(dT) primers. Biotin-labeled cRNA was generated by in vitro transcription reactions using ENZO labeling kit, fragmented and then 20 micrograms of fragmented cRNA were hybridized to the arrays according to Affymetrix instructions.

source_name: Ecotype Columbia, Light grown 7-day old seedlings


title: 11552B2

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 63
Sample_probe_id: 469


title: 15208Q

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 51
Sample_probe_id: 451


title: 15208R

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 53
Sample_probe_id: 451


title: 15208P

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 55
Sample_probe_id: 451


title: 15208I2

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 29
Sample_probe_id: 471


title: 11552Q

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 173
Sample_probe_id: 451


title: 11552R

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 71
Sample_probe_id: 451


title: 07886M

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 676
Sample_probe_id: 450


title: 15208J2

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 26
Sample_probe_id: 471


title: 07886O

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 364
Sample_probe_id: 450


title: 07886N

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 269
Sample_probe_id: 450


title: 11548X

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 113
Sample_probe_id: 467


title: 11552P

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 191
Sample_probe_id: 451


title: 15208H2

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 31
Sample_probe_id: 471


title: 11552X

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 78
Sample_probe_id: 467


title: 11552J2

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 22
Sample_probe_id: 471


title: 11552I2

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 24
Sample_probe_id: 471


title: 11548W

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 55
Sample_probe_id: 467


title: 07886X

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 28
Sample_probe_id: 467


title: 11548I

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 76
Sample_probe_id: 448


title: 07886V


title: 15208C

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 34
Sample_probe_id: 446


title: 15208B

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 57
Sample_probe_id: 446


title: 07886i

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 90
Sample_probe_id: 448


title: 07886W

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 72
Sample_probe_id: 467


title: 11552H2

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 25
Sample_probe_id: 471


title: 11548H

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 96
Sample_probe_id: 448


title: 15208A

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 63
Sample_probe_id: 446


title: 11552C

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 48
Sample_probe_id: 446


title: 11552B

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 53
Sample_probe_id: 446


title: 07886H

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 80
Sample_probe_id: 448


title: 11548F

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 86
Sample_probe_id: 447


title: 11548G

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 95
Sample_probe_id: 448


title: 07886G

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 77
Sample_probe_id: 448


title: 11548E

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 114
Sample_probe_id: 447


title: 07886F

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 74
Sample_probe_id: 447


title: 07886D

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 66
Sample_probe_id: 447


title: 07886E

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 64
Sample_probe_id: 447


title: 15208F2

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 60
Sample_probe_id: 470


title: 11552A

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 54
Sample_probe_id: 446


title: 15208G2

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 32
Sample_probe_id: 470


title: 11548V

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 59
Sample_probe_id: 467


title: 11548B2

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 109
Sample_probe_id: 469


title: 11548D2

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 133
Sample_probe_id: 469


title: Reciprocity-8 h low UV-B-2

city: Rosario

first: Paula

last: Casati

state: Santa Fe

address: Suipacha 531

department: Biological Sciences

country: Argentina

institute: Universidad Nacional de Rosario

description: Microarray hybridization was used to assess UV-B responses in both directly exposed and shielded tissues of a maize line deficient in anthocyanin accumulation.  Among 347 transcripts found to be regulated after 8 h of high UV-B irradiation, 285 were increased significantly in at least one organ but only 80 were down-regulated by UV-B.  Although some genes were regulated by UV-B in more than one sample, most UV-B regulated genes were organ-specific.  A larger number of transcript changes occurred in directly exposed than in shielded organs, and more transcripts levels were changed in adult than in seedling tissues.  Because shielded tissues including roots, immature ears, and leaves covered with a plastic that absorbs UV-B exhibited altered transcriptome profiles after plant exposure to UV-B, some signal(s) must be transmitted from irradiated to shielded tissues.   The time course of transcript abundance changes monitored by RNA blot hybridization and real time RT-PCR indicated that the response kinetics to UV-B are very rapid as some transcript levels are altered within 1 h exposure, in both exposed and shielded tissues.   As the same total UV-B irradiation dose applied at three intensities elicited different transcript profiles, transcriptome changes do not show reciprocity.  Confirming and extending prior microarray hybridization results, supplemental UV-B increased expression of stress response and ribosomal protein genes, while photosynthesis-associated genes were down regulated in adult leaves.

organism: Zea mays

source_name: control

source_name: UV-B exposure


title: 11548D

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 89
Sample_probe_id: 447


title: Reciprocity-8h high UV-B-2


title: Reciprocity-8h low UV-B

source_name: UV-B exposed


title: Reciprocity-8h high UV-B


title: 11552G2

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 24
Sample_probe_id: 470


title: 07886B2

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 50
Sample_probe_id: 469


title: 11548R

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 59
Sample_probe_id: 451


title: 11552F2


title: 15208E2

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 31
Sample_probe_id: 470


title: 15208Z

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 26
Sample_probe_id: 468


title: 07886D2

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 48
Sample_probe_id: 469


title: Reciprocity-4h medium UV-B


title: 15208Y

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 21
Sample_probe_id: 468


title: Reciprocity-2h high UV-B


title: 07886Q

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 83
Sample_probe_id: 451


title: 11548Q

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 63
Sample_probe_id: 451


title: 15208A2

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 25
Sample_probe_id: 468


title: 11552E2


title: 07886C2

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 27
Sample_probe_id: 469


title: 11548J2

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 148
Sample_probe_id: 471


title: 07886J2

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 61
Sample_probe_id: 471


title: 11548C2

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 137
Sample_probe_id: 469


title: reciprocity-2h high UV-B-2


title: 11548I2

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 159
Sample_probe_id: 471


title: 11548H2

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 173
Sample_probe_id: 471


title: 07886i2

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 76
Sample_probe_id: 471


title: 07886P

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 77
Sample_probe_id: 451


title: tassel-8h UV-B

description: Microarray hybridization was used to assess UV-B responses in both directly exposed and shielded tissues of a maize line deficient in anthocyanin accumulation.  Among 347 transcripts found to be regulated after 8 h of high UV-B irradiation, 285 were increased significantly in at least one organ but only 80 were down-regulated by UV-B.  Although some genes were regulated by UV-B in more than one sample, most UV-B regulated genes were organ-specific.  A larger number of transcript changes occurred in directly exposed than in shielded organs, and more transcripts levels were changed in adult than in seedling tissues.  Because shielded tissues including roots, immature ears, and leaves covered with a plastic that absorbs UV-B exhibited altered transcriptome profiles after plant exposure to UV-B, some signal(s) must be transmitted from irradiated to shielded tissues.   The time course of transcript abundance changes monitored by RNA blot hybridization and real time RT-PCR indicated that the response kinetics to UV-B are very rapid as some transcript levels are altered within 1 h exposure, in both exposed and shielded tissues.   As the same total UV-B irradiation dose applied at three intensities elicited different transcript profiles, transcriptome changes do not show reciprocity.  Confirming and extending prior microarray hybridization results, supplemental UV-B increased expression of stress response and ribosomal protein genes, while photosynthesis-associated genes were down regulated in adult leaves.


title: immature ear-8h UV-B exposure


title: 11552A2

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 77
Sample_probe_id: 468


title: 07886R

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 82
Sample_probe_id: 451


title: immature ear-8H UV-B


title: 11552Z


title: 11548C

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 123
Sample_probe_id: 446


title: 07886H2

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 56
Sample_probe_id: 471


title: adult leaf-PE covered leaf in UV-B exposed plants vs PE covered leaf in control plants

source_name: PE covered leaf in UV-B exposed plants

source_name: PE covered leaf in control plants


title: 11552Y

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 20
Sample_probe_id: 468


title: 07886C

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 106
Sample_probe_id: 446


title: 11552U

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 71
Sample_probe_id: 466


title: 11548A


title: 15208T

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 26
Sample_probe_id: 466


title: 07886B

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 114
Sample_probe_id: 446


title: 07886G2

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 30
Sample_probe_id: 470


title: adult leaf-CA bag vs PE bag in UV-B exposed leaves

source_name: PE bag

source_name: CA bag


title: adult leaf-8h UV-B-2

source_name: control leaf

source_name: UV-B leaf


title: 15208S

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 23
Sample_probe_id: 466


title: roots-8h UV-B

source_name: UV-B exposed plants

source_name: control plants


title: 11548E2

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 149
Sample_probe_id: 470


title: 11548F2

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 139
Sample_probe_id: 470


title: 11548B

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 125
Sample_probe_id: 446


title: 07886A

description:
Probe Generation
Probes were generated from three independent BV2 cultures treated with freshly sonicated Abeta-42 peptide (California Peptide) along with controls. Briefly, mRNA was isolated from those cells as described previously.  Double-stranded cDNA (ds-cDNA) was generated by reverse transcription and second-strand synthesis, and digested with RsaI (New England Biolabs, Beverly, MA). Probes were generated through labeling with 33P-dCTP (Perkin Elmer Lifesciences, Boston, MA) using a Decamer labeling kit (Ambion, Austin, Texas).
Filter Hybridization
Hybridization of probes to filters was performed at 42.C for 24 hr in a hybridization solution containing 50% formamide.
Data Collection
A Cyclone phosphorimaging system (Packard Biosciences, Meriden, CT) and GenePix software analysis (GenePix Pro 3.0.6.24, Axon Instruments, Foster City, CA) were used to obtain densitometry of the 33P signal.
Sample_normalization_factor: 104
Sample_probe_id: 446