title: 923_171

status: Public on May 01 2003

first: David

last: Rasko

address: 9712 Medical Center Drive

department: Microbial Genomics

institute: The Institute for Genomic Research

description:
Bacillus cereus strain ATCC 14579, type strain, sequenced at Integrated Genomics/INRA
Amplicons representing 79 of 217 and 41 of 122 genes from pXO1 and pXO2 respectively, and 3601 of 5753 chromosomal genes as predicted by Glimmer 30 ( and see supplemental methods) were arrayed onto glass microscope slides (Telechem, Inc). Redundant genes were generally represented once or a few times on the array.
Genomic DNA was labeled with Cy3 and Cy5 according to J. DeRisi (http://www.microarrays.org/Pdfs/GenomicDNALabel_B.pdf), except that genomic DNA was not digested or sheared before labeling.
Arrays were scanned with a GenePix 4000B scanner (Axon Inc.).
Hybridization signals were quantitated using TIGR SPOTFINDER (software available at http://www.tigr.org/softlab).
Hybridization experiments were competitive using probes derived from B. anthracis Ames (reference) and a B. cereus group (query) strain. Normalized signal intensities were used to generate relative hybridization ratios (query/reference). Data representing weak signal were removed. The ratios from a maximum of six data points (duplicate spots, hybridizations performed in triplicate) were placed in three bins (<0.1) gene is absent in query strain, (0.1-0.3) present but diverged in query strain, and (>0.3) gene is present in the query strain. A majority rule was applied to the data for binning such that more than 50% of ratios were in agreement as to assignment and that at least two data points were used (exceeded in 99% of the cases). In cases where less than two data points existed, the gene was treated as data missing.
The criteria for the numerical ranges of our bins were established in two ways. First, we determined the presence or absence of sequences homologous to 3601 B. anthracis genes in the sequence of B. cereus ATCC 14579 (Integrated Genomics, Inc; http://www.integratedgenomics.com/) using BLASTN and compared that to the assignments inferred from hybridization ratios. A threshold of 0.1 was found to be suitable for classifying a gene as absent (i.e., agreement between sequence and CGH data in 99% of the cases), while a cutoff value of 0.3 was conservative for gene presence (agreement in 92% of the cases). Second, we used a set of 65 genes conserved in 26 bacterial genomes, NCBI COG database (http://www.ncbi.nlm.nih.gov/COG/). Genes judged as present in query strains using our selected cut-offs correctly binned data in 1225 out of 1235 total calls. There was a tendency for underprediction of plasmid homologs by CGH, when compared to results from the sequence analysis. Two possible explanations for this are variability in plasmid copy number in B. cereus strains relative to B. anthracis and/or that the average divergence of plasmid genes is greater than chromosomal genes.and that at least two data points were used (exceeded in 99% of the cases)

organism:
Bacillus anthracis
Bacillus cereus

source_name: Bacillus cereus strain ATCC 14579

source_name: Bacillus anthracis Ames


title: 923_311

type: genomic

city: Rockville

state: MD

country: USA

molecule: genomic DNA


title: H1a, Total RNA

status: Public on May 27 2003

type: RNA

first: Jo Ann

middle: S.

last: Rinaudo

address: 13 Taft Court, Suite 101

department: Microbiology

institute: Georgetown University

description:
Total RNA from adult human and woodchuck
liver was analyzed on human membrane arrays.
Two human and two woodchuck liver samples
were analyzed in duplicate.  The samples were
numbered H1a, H1b, H2a, H2b, WC1a, WC1b,
WC2a, and WC2b.  H, represents human liver;
WC represents woodchuck liver.  The numbers
1 and 2 were the two individual samples. The
a and b represent replicates of the same RNA
sample.
Keywords = liver, adult, normal, human
Lot batch = 1100638

molecule: total RNA

organism: Homo sapiens

source_name: Total RNA - liver


title: 874_364

description:
Bacillus cereus strain 874
Amplicons representing 79 of 217 and 41 of 122 genes from pXO1 and pXO2 respectively, and 3601 of 5753 chromosomal genes as predicted by Glimmer 30 ( and see supplemental methods) were arrayed onto glass microscope slides (Telechem, Inc). Redundant genes were generally represented once or a few times on the array.
Genomic DNA was labeled with Cy3 and Cy5 according to J. DeRisi (http://www.microarrays.org/Pdfs/GenomicDNALabel_B.pdf), except that genomic DNA was not digested or sheared before labeling.
Arrays were scanned with a GenePix 4000B scanner (Axon Inc.).
Hybridization signals were quantitated using TIGR SPOTFINDER (software available at http://www.tigr.org/softlab).
Hybridization experiments were competitive using probes derived from B. anthracis Ames (reference) and a B. cereus group (query) strain. Normalized signal intensities were used to generate relative hybridization ratios (query/reference). Data representing weak signal were removed. The ratios from a maximum of six data points (duplicate spots, hybridizations performed in triplicate) were placed in three bins (<0.1) gene is absent in query strain, (0.1-0.3) present but diverged in query strain, and (>0.3) gene is present in the query strain. A majority rule was applied to the data for binning such that more than 50% of ratios were in agreement as to assignment and that at least two data points were used (exceeded in 99% of the cases). In cases where less than two data points existed, the gene was treated as data missing.
The criteria for the numerical ranges of our bins were established in two ways. First, we determined the presence or absence of sequences homologous to 3601 B. anthracis genes in the sequence of B. cereus ATCC 14579 (Integrated Genomics, Inc; http://www.integratedgenomics.com/) using BLASTN and compared that to the assignments inferred from hybridization ratios. A threshold of 0.1 was found to be suitable for classifying a gene as absent (i.e., agreement between sequence and CGH data in 99% of the cases), while a cutoff value of 0.3 was conservative for gene presence (agreement in 92% of the cases). Second, we used a set of 65 genes conserved in 26 bacterial genomes, NCBI COG database (http://www.ncbi.nlm.nih.gov/COG/). Genes judged as present in query strains using our selected cut-offs correctly binned data in 1225 out of 1235 total calls. There was a tendency for underprediction of plasmid homologs by CGH, when compared to results from the sequence analysis. Two possible explanations for this are variability in plasmid copy number in B. cereus strains relative to B. anthracis and/or that the average divergence of plasmid genes is greater than chromosomal genes.and that at least two data points were used (exceeded in 99% of the cases)

source_name: Bacillus cereus strain 874


title: 923_256


title: 874_340


title: 840_290

description:
Bacillus thuringiensis strain 840
Amplicons representing 79 of 217 and 41 of 122 genes from pXO1 and pXO2 respectively, and 3601 of 5753 chromosomal genes as predicted by Glimmer 30 ( and see supplemental methods) were arrayed onto glass microscope slides (Telechem, Inc). Redundant genes were generally represented once or a few times on the array.
Genomic DNA was labeled with Cy3 and Cy5 according to J. DeRisi (http://www.microarrays.org/Pdfs/GenomicDNALabel_B.pdf), except that genomic DNA was not digested or sheared before labeling.
Arrays were scanned with a GenePix 4000B scanner (Axon Inc.).
Hybridization signals were quantitated using TIGR SPOTFINDER (software available at http://www.tigr.org/softlab).
Hybridization experiments were competitive using probes derived from B. anthracis Ames (reference) and a B. cereus group (query) strain. Normalized signal intensities were used to generate relative hybridization ratios (query/reference). Data representing weak signal were removed. The ratios from a maximum of six data points (duplicate spots, hybridizations performed in triplicate) were placed in three bins (<0.1) gene is absent in query strain, (0.1-0.3) present but diverged in query strain, and (>0.3) gene is present in the query strain. A majority rule was applied to the data for binning such that more than 50% of ratios were in agreement as to assignment and that at least two data points were used (exceeded in 99% of the cases). In cases where less than two data points existed, the gene was treated as data missing.
The criteria for the numerical ranges of our bins were established in two ways. First, we determined the presence or absence of sequences homologous to 3601 B. anthracis genes in the sequence of B. cereus ATCC 14579 (Integrated Genomics, Inc; http://www.integratedgenomics.com/) using BLASTN and compared that to the assignments inferred from hybridization ratios. A threshold of 0.1 was found to be suitable for classifying a gene as absent (i.e., agreement between sequence and CGH data in 99% of the cases), while a cutoff value of 0.3 was conservative for gene presence (agreement in 92% of the cases). Second, we used a set of 65 genes conserved in 26 bacterial genomes, NCBI COG database (http://www.ncbi.nlm.nih.gov/COG/). Genes judged as present in query strains using our selected cut-offs correctly binned data in 1225 out of 1235 total calls. There was a tendency for underprediction of plasmid homologs by CGH, when compared to results from the sequence analysis. Two possible explanations for this are variability in plasmid copy number in B. cereus strains relative to B. anthracis and/or that the average divergence of plasmid genes is greater than chromosomal genes.and that at least two data points were used (exceeded in 99% of the cases)

organism:
Bacillus anthracis
Bacillus thuringiensis

source_name: Bacillus thuringiensis strain 840


title: 874_367


title: 923_222


title: 831_240

description:
Bacillus cereus strain 831; Isolated April 1996 in Norway, Oslo, from patient (Female, 45) with periodontal pockets and marginal periodontitis, Serotype H18
Amplicons representing 79 of 217 and 41 of 122 genes from pXO1 and pXO2 respectively, and 3601 of 5753 chromosomal genes as predicted by Glimmer 30 ( and see supplemental methods) were arrayed onto glass microscope slides (Telechem, Inc). Redundant genes were generally represented once or a few times on the array.
Genomic DNA was labeled with Cy3 and Cy5 according to J. DeRisi (http://www.microarrays.org/Pdfs/GenomicDNALabel_B.pdf), except that genomic DNA was not digested or sheared before labeling.
Arrays were scanned with a GenePix 4000B scanner (Axon Inc.).
Hybridization signals were quantitated using TIGR SPOTFINDER (software available at http://www.tigr.org/softlab).
Hybridization experiments were competitive using probes derived from B. anthracis Ames (reference) and a B. cereus group (query) strain. Normalized signal intensities were used to generate relative hybridization ratios (query/reference). Data representing weak signal were removed. The ratios from a maximum of six data points (duplicate spots, hybridizations performed in triplicate) were placed in three bins (<0.1) gene is absent in query strain, (0.1-0.3) present but diverged in query strain, and (>0.3) gene is present in the query strain. A majority rule was applied to the data for binning such that more than 50% of ratios were in agreement as to assignment and that at least two data points were used (exceeded in 99% of the cases). In cases where less than two data points existed, the gene was treated as data missing.
The criteria for the numerical ranges of our bins were established in two ways. First, we determined the presence or absence of sequences homologous to 3601 B. anthracis genes in the sequence of B. cereus ATCC 14579 (Integrated Genomics, Inc; http://www.integratedgenomics.com/) using BLASTN and compared that to the assignments inferred from hybridization ratios. A threshold of 0.1 was found to be suitable for classifying a gene as absent (i.e., agreement between sequence and CGH data in 99% of the cases), while a cutoff value of 0.3 was conservative for gene presence (agreement in 92% of the cases). Second, we used a set of 65 genes conserved in 26 bacterial genomes, NCBI COG database (http://www.ncbi.nlm.nih.gov/COG/). Genes judged as present in query strains using our selected cut-offs correctly binned data in 1225 out of 1235 total calls. There was a tendency for underprediction of plasmid homologs by CGH, when compared to results from the sequence analysis. Two possible explanations for this are variability in plasmid copy number in B. cereus strains relative to B. anthracis and/or that the average divergence of plasmid genes is greater than chromosomal genes.and that at least two data points were used (exceeded in 99% of the cases)

source_name: Bacillus cereus strain 831


title: 816_358

description:
Bacillus cereus strain 816; Isolated November 1995 in Norway, Akershus from patient (Male, 35) with granuloma
Amplicons representing 79 of 217 and 41 of 122 genes from pXO1 and pXO2 respectively, and 3601 of 5753 chromosomal genes as predicted by Glimmer 30 ( and see supplemental methods) were arrayed onto glass microscope slides (Telechem, Inc). Redundant genes were generally represented once or a few times on the array.
Genomic DNA was labeled with Cy3 and Cy5 according to J. DeRisi (http://www.microarrays.org/Pdfs/GenomicDNALabel_B.pdf), except that genomic DNA was not digested or sheared before labeling.
Arrays were scanned with a GenePix 4000B scanner (Axon Inc.).
Hybridization signals were quantitated using TIGR SPOTFINDER (software available at http://www.tigr.org/softlab).
Hybridization experiments were competitive using probes derived from B. anthracis Ames (reference) and a B. cereus group (query) strain. Normalized signal intensities were used to generate relative hybridization ratios (query/reference). Data representing weak signal were removed. The ratios from a maximum of six data points (duplicate spots, hybridizations performed in triplicate) were placed in three bins (<0.1) gene is absent in query strain, (0.1-0.3) present but diverged in query strain, and (>0.3) gene is present in the query strain. A majority rule was applied to the data for binning such that more than 50% of ratios were in agreement as to assignment and that at least two data points were used (exceeded in 99% of the cases). In cases where less than two data points existed, the gene was treated as data missing.
The criteria for the numerical ranges of our bins were established in two ways. First, we determined the presence or absence of sequences homologous to 3601 B. anthracis genes in the sequence of B. cereus ATCC 14579 (Integrated Genomics, Inc; http://www.integratedgenomics.com/) using BLASTN and compared that to the assignments inferred from hybridization ratios. A threshold of 0.1 was found to be suitable for classifying a gene as absent (i.e., agreement between sequence and CGH data in 99% of the cases), while a cutoff value of 0.3 was conservative for gene presence (agreement in 92% of the cases). Second, we used a set of 65 genes conserved in 26 bacterial genomes, NCBI COG database (http://www.ncbi.nlm.nih.gov/COG/). Genes judged as present in query strains using our selected cut-offs correctly binned data in 1225 out of 1235 total calls. There was a tendency for underprediction of plasmid homologs by CGH, when compared to results from the sequence analysis. Two possible explanations for this are variability in plasmid copy number in B. cereus strains relative to B. anthracis and/or that the average divergence of plasmid genes is greater than chromosomal genes.and that at least two data points were used (exceeded in 99% of the cases)

source_name: Bacillus cereus strain 816


title: 819_230

description:
Bacillus cereus strain 819; [OH 600] Isolated September 1995 in Norway, Oslo, from patient (Male, 48) with periodontal pockets and apical periodontitis, Serotype H2-
Amplicons representing 79 of 217 and 41 of 122 genes from pXO1 and pXO2 respectively, and 3601 of 5753 chromosomal genes as predicted by Glimmer 30 ( and see supplemental methods) were arrayed onto glass microscope slides (Telechem, Inc). Redundant genes were generally represented once or a few times on the array.
Genomic DNA was labeled with Cy3 and Cy5 according to J. DeRisi (http://www.microarrays.org/Pdfs/GenomicDNALabel_B.pdf), except that genomic DNA was not digested or sheared before labeling.
Arrays were scanned with a GenePix 4000B scanner (Axon Inc.).
Hybridization signals were quantitated using TIGR SPOTFINDER (software available at http://www.tigr.org/softlab).
Hybridization experiments were competitive using probes derived from B. anthracis Ames (reference) and a B. cereus group (query) strain. Normalized signal intensities were used to generate relative hybridization ratios (query/reference). Data representing weak signal were removed. The ratios from a maximum of six data points (duplicate spots, hybridizations performed in triplicate) were placed in three bins (<0.1) gene is absent in query strain, (0.1-0.3) present but diverged in query strain, and (>0.3) gene is present in the query strain. A majority rule was applied to the data for binning such that more than 50% of ratios were in agreement as to assignment and that at least two data points were used (exceeded in 99% of the cases). In cases where less than two data points existed, the gene was treated as data missing.
The criteria for the numerical ranges of our bins were established in two ways. First, we determined the presence or absence of sequences homologous to 3601 B. anthracis genes in the sequence of B. cereus ATCC 14579 (Integrated Genomics, Inc; http://www.integratedgenomics.com/) using BLASTN and compared that to the assignments inferred from hybridization ratios. A threshold of 0.1 was found to be suitable for classifying a gene as absent (i.e., agreement between sequence and CGH data in 99% of the cases), while a cutoff value of 0.3 was conservative for gene presence (agreement in 92% of the cases). Second, we used a set of 65 genes conserved in 26 bacterial genomes, NCBI COG database (http://www.ncbi.nlm.nih.gov/COG/). Genes judged as present in query strains using our selected cut-offs correctly binned data in 1225 out of 1235 total calls. There was a tendency for underprediction of plasmid homologs by CGH, when compared to results from the sequence analysis. Two possible explanations for this are variability in plasmid copy number in B. cereus strains relative to B. anthracis and/or that the average divergence of plasmid genes is greater than chromosomal genes.and that at least two data points were used (exceeded in 99% of the cases)

source_name: Bacillus cereus strain 819


title: 816_332


title: 840_219


title: 819_181


title: 819_175


title: 816_339


title: 831_213


title: 812_286

description:
Bacillus cereus strain 812; [OH 599] Isolated September 1995 in Norway, Oslo, from patient (Male, 48) with root canal and apical periodontitis, Serotype H-
Amplicons representing 79 of 217 and 41 of 122 genes from pXO1 and pXO2 respectively, and 3601 of 5753 chromosomal genes as predicted by Glimmer 30 ( and see supplemental methods) were arrayed onto glass microscope slides (Telechem, Inc). Redundant genes were generally represented once or a few times on the array.
Genomic DNA was labeled with Cy3 and Cy5 according to J. DeRisi (http://www.microarrays.org/Pdfs/GenomicDNALabel_B.pdf), except that genomic DNA was not digested or sheared before labeling.
Arrays were scanned with a GenePix 4000B scanner (Axon Inc.).
Hybridization signals were quantitated using TIGR SPOTFINDER (software available at http://www.tigr.org/softlab).
Hybridization experiments were competitive using probes derived from B. anthracis Ames (reference) and a B. cereus group (query) strain. Normalized signal intensities were used to generate relative hybridization ratios (query/reference). Data representing weak signal were removed. The ratios from a maximum of six data points (duplicate spots, hybridizations performed in triplicate) were placed in three bins (<0.1) gene is absent in query strain, (0.1-0.3) present but diverged in query strain, and (>0.3) gene is present in the query strain. A majority rule was applied to the data for binning such that more than 50% of ratios were in agreement as to assignment and that at least two data points were used (exceeded in 99% of the cases). In cases where less than two data points existed, the gene was treated as data missing.
The criteria for the numerical ranges of our bins were established in two ways. First, we determined the presence or absence of sequences homologous to 3601 B. anthracis genes in the sequence of B. cereus ATCC 14579 (Integrated Genomics, Inc; http://www.integratedgenomics.com/) using BLASTN and compared that to the assignments inferred from hybridization ratios. A threshold of 0.1 was found to be suitable for classifying a gene as absent (i.e., agreement between sequence and CGH data in 99% of the cases), while a cutoff value of 0.3 was conservative for gene presence (agreement in 92% of the cases). Second, we used a set of 65 genes conserved in 26 bacterial genomes, NCBI COG database (http://www.ncbi.nlm.nih.gov/COG/). Genes judged as present in query strains using our selected cut-offs correctly binned data in 1225 out of 1235 total calls. There was a tendency for underprediction of plasmid homologs by CGH, when compared to results from the sequence analysis. Two possible explanations for this are variability in plasmid copy number in B. cereus strains relative to B. anthracis and/or that the average divergence of plasmid genes is greater than chromosomal genes.and that at least two data points were used (exceeded in 99% of the cases)

source_name: Bacillus cereus strain 812


title: Testes of hpg mice T+T (1)

status: Public on May 28 2003

city: Pullman

first: Patricia

middle: Imele

last: Sadate-Ngatchou

state: WA

department: School of Molecular Biosciences

institute: Washington State University

description:
Transcriptome of testes of hypogonadal mice that had been pretreated with testosterone proprionate (TP), submitted to testosterone withdrawal then treated with TP for 4 hour.
Keywords = testis, hypogonal mice, testosterone

organism: Mus musculus

source_name: testis


title: 812_174


title: 812_180


title: 775_310

description:
Amplicons representing 79 of 217 and 41 of 122 genes from pXO1 and pXO2 respectively, and 3601 of 5753 chromosomal genes as predicted by Glimmer 30 ( and see supplemental methods) were arrayed onto glass microscope slides (Telechem, Inc). Redundant genes were generally represented once or a few times on the array.
Genomic DNA was labeled with Cy3 and Cy5 according to J. DeRisi (http://www.microarrays.org/Pdfs/GenomicDNALabel_B.pdf), except that genomic DNA was not digested or sheared before labeling.
Arrays were scanned with a GenePix 4000B scanner (Axon Inc.).
Hybridization signals were quantitated using TIGR SPOTFINDER (software available at http://www.tigr.org/softlab).
Hybridization experiments were competitive using probes derived from B. anthracis Ames (reference) and a B. cereus group (query) strain. Normalized signal intensities were used to generate relative hybridization ratios (query/reference). Data representing weak signal were removed. The ratios from a maximum of six data points (duplicate spots, hybridizations performed in triplicate) were placed in three bins (<0.1) gene is absent in query strain, (0.1-0.3) present but diverged in query strain, and (>0.3) gene is present in the query strain. A majority rule was applied to the data for binning such that more than 50% of ratios were in agreement as to assignment and that at least two data points were used (exceeded in 99% of the cases). In cases where less than two data points existed, the gene was treated as data missing.
The criteria for the numerical ranges of our bins were established in two ways. First, we determined the presence or absence of sequences homologous to 3601 B. anthracis genes in the sequence of B. cereus ATCC 14579 (Integrated Genomics, Inc; http://www.integratedgenomics.com/) using BLASTN and compared that to the assignments inferred from hybridization ratios. A threshold of 0.1 was found to be suitable for classifying a gene as absent (i.e., agreement between sequence and CGH data in 99% of the cases), while a cutoff value of 0.3 was conservative for gene presence (agreement in 92% of the cases). Second, we used a set of 65 genes conserved in 26 bacterial genomes, NCBI COG database (http://www.ncbi.nlm.nih.gov/COG/). Genes judged as present in query strains using our selected cut-offs correctly binned data in 1225 out of 1235 total calls. There was a tendency for underprediction of plasmid homologs by CGH, when compared to results from the sequence analysis. Two possible explanations for this are variability in plasmid copy number in B. cereus strains relative to B. anthracis and/or that the average divergence of plasmid genes is greater than chromosomal genes.and that at least two data points were used (exceeded in 99% of the cases)

source_name: Bacillus thuringiensis strain 775


title: 840_227


title: 775_216


title: 775_165


title: 612_231

description:
Bacillus cereus strain 612; Isolated in Norway, Orkdal, from a Dairy, Serotype H34
Amplicons representing 79 of 217 and 41 of 122 genes from pXO1 and pXO2 respectively, and 3601 of 5753 chromosomal genes as predicted by Glimmer 30 ( and see supplemental methods) were arrayed onto glass microscope slides (Telechem, Inc). Redundant genes were generally represented once or a few times on the array.
Genomic DNA was labeled with Cy3 and Cy5 according to J. DeRisi (http://www.microarrays.org/Pdfs/GenomicDNALabel_B.pdf), except that genomic DNA was not digested or sheared before labeling.
Arrays were scanned with a GenePix 4000B scanner (Axon Inc.).
Hybridization signals were quantitated using TIGR SPOTFINDER (software available at http://www.tigr.org/softlab).
Hybridization experiments were competitive using probes derived from B. anthracis Ames (reference) and a B. cereus group (query) strain. Normalized signal intensities were used to generate relative hybridization ratios (query/reference). Data representing weak signal were removed. The ratios from a maximum of six data points (duplicate spots, hybridizations performed in triplicate) were placed in three bins (<0.1) gene is absent in query strain, (0.1-0.3) present but diverged in query strain, and (>0.3) gene is present in the query strain. A majority rule was applied to the data for binning such that more than 50% of ratios were in agreement as to assignment and that at least two data points were used (exceeded in 99% of the cases). In cases where less than two data points existed, the gene was treated as data missing.
The criteria for the numerical ranges of our bins were established in two ways. First, we determined the presence or absence of sequences homologous to 3601 B. anthracis genes in the sequence of B. cereus ATCC 14579 (Integrated Genomics, Inc; http://www.integratedgenomics.com/) using BLASTN and compared that to the assignments inferred from hybridization ratios. A threshold of 0.1 was found to be suitable for classifying a gene as absent (i.e., agreement between sequence and CGH data in 99% of the cases), while a cutoff value of 0.3 was conservative for gene presence (agreement in 92% of the cases). Second, we used a set of 65 genes conserved in 26 bacterial genomes, NCBI COG database (http://www.ncbi.nlm.nih.gov/COG/). Genes judged as present in query strains using our selected cut-offs correctly binned data in 1225 out of 1235 total calls. There was a tendency for underprediction of plasmid homologs by CGH, when compared to results from the sequence analysis. Two possible explanations for this are variability in plasmid copy number in B. cereus strains relative to B. anthracis and/or that the average divergence of plasmid genes is greater than chromosomal genes.and that at least two data points were used (exceeded in 99% of the cases)

source_name: Bacillus cereus strain 612


title: 607_260

description:
Bacillus cereus strain 607; Isolated in Norway, Tynset, from a Dairy, Serotype H-
Amplicons representing 79 of 217 and 41 of 122 genes from pXO1 and pXO2 respectively, and 3601 of 5753 chromosomal genes as predicted by Glimmer 30 ( and see supplemental methods) were arrayed onto glass microscope slides (Telechem, Inc). Redundant genes were generally represented once or a few times on the array.
Genomic DNA was labeled with Cy3 and Cy5 according to J. DeRisi (http://www.microarrays.org/Pdfs/GenomicDNALabel_B.pdf), except that genomic DNA was not digested or sheared before labeling.
Arrays were scanned with a GenePix 4000B scanner (Axon Inc.).
Hybridization signals were quantitated using TIGR SPOTFINDER (software available at http://www.tigr.org/softlab).
Hybridization experiments were competitive using probes derived from B. anthracis Ames (reference) and a B. cereus group (query) strain. Normalized signal intensities were used to generate relative hybridization ratios (query/reference). Data representing weak signal were removed. The ratios from a maximum of six data points (duplicate spots, hybridizations performed in triplicate) were placed in three bins (<0.1) gene is absent in query strain, (0.1-0.3) present but diverged in query strain, and (>0.3) gene is present in the query strain. A majority rule was applied to the data for binning such that more than 50% of ratios were in agreement as to assignment and that at least two data points were used (exceeded in 99% of the cases). In cases where less than two data points existed, the gene was treated as data missing.
The criteria for the numerical ranges of our bins were established in two ways. First, we determined the presence or absence of sequences homologous to 3601 B. anthracis genes in the sequence of B. cereus ATCC 14579 (Integrated Genomics, Inc; http://www.integratedgenomics.com/) using BLASTN and compared that to the assignments inferred from hybridization ratios. A threshold of 0.1 was found to be suitable for classifying a gene as absent (i.e., agreement between sequence and CGH data in 99% of the cases), while a cutoff value of 0.3 was conservative for gene presence (agreement in 92% of the cases). Second, we used a set of 65 genes conserved in 26 bacterial genomes, NCBI COG database (http://www.ncbi.nlm.nih.gov/COG/). Genes judged as present in query strains using our selected cut-offs correctly binned data in 1225 out of 1235 total calls. There was a tendency for underprediction of plasmid homologs by CGH, when compared to results from the sequence analysis. Two possible explanations for this are variability in plasmid copy number in B. cereus strains relative to B. anthracis and/or that the average divergence of plasmid genes is greater than chromosomal genes.and that at least two data points were used (exceeded in 99% of the cases)

source_name: Bacillus cereus strain 607


title: 535_164

source_name: Bacillus cereus strain 535


title: 535_215


title: 535_238


title: 775_239


title: 607_232


title: 612_287


title: 607_273


title: 612_272


title: 442_217

source_name: Bacillus thuringiensis strain 442


title: 442_225


title: 442_169


title: 259_278

source_name: Bacillus cereus strain 259


title: 259_262


title: 251_276

source_name: Bacillus thuringiensis strain 251


title: Regulated genes in diabetic placentas

status: Public on Apr 30 2003

city: Paris

first: Sylvie

last: Hauguel-de Mouzon

address: 24 rue de Chaligny

department: Hopital Saint Antoine

country: France

institute: Universite Paris6

description: Quantitative modifications of up- and down-regulated genes in diabetic placentas.

source_name: human placenta


title: 251_288

source_name: Bacillus anthracis Ames


title: experiment XI, hTERT

status: Public on Apr 26 2003

city: Los Angeles

first: Hilary

last: Coller

state: CA

address: Terasaki Life Science Building 5145, 610 Charles E Young Drive E

institute: University of California, Los Angeles

description: mammary epithelial cells transduced with hTERT

source_name: mammary epithelial cells


title: 259_308


title: 1143_345

description:
NOTE: Bacillus weihenstephanensis
Amplicons representing 79 of 217 and 41 of 122 genes from pXO1 and pXO2 respectively, and 3601 of 5753 chromosomal genes as predicted by Glimmer 30 ( and see supplemental methods) were arrayed onto glass microscope slides (Telechem, Inc). Redundant genes were generally represented once or a few times on the array.
Genomic DNA was labeled with Cy3 and Cy5 according to J. DeRisi (http://www.microarrays.org/Pdfs/GenomicDNALabel_B.pdf), except that genomic DNA was not digested or sheared before labeling.
Arrays were scanned with a GenePix 4000B scanner (Axon Inc.).
Hybridization signals were quantitated using TIGR SPOTFINDER (software available at http://www.tigr.org/softlab).
Hybridization experiments were competitive using probes derived from B. anthracis Ames (reference) and a B. cereus group (query) strain. Normalized signal intensities were used to generate relative hybridization ratios (query/reference). Data representing weak signal were removed. The ratios from a maximum of six data points (duplicate spots, hybridizations performed in triplicate) were placed in three bins (<0.1) gene is absent in query strain, (0.1-0.3) present but diverged in query strain, and (>0.3) gene is present in the query strain. A majority rule was applied to the data for binning such that more than 50% of ratios were in agreement as to assignment and that at least two data points were used (exceeded in 99% of the cases). In cases where less than two data points existed, the gene was treated as data missing.
The criteria for the numerical ranges of our bins were established in two ways. First, we determined the presence or absence of sequences homologous to 3601 B. anthracis genes in the sequence of B. cereus ATCC 14579 (Integrated Genomics, Inc; http://www.integratedgenomics.com/) using BLASTN and compared that to the assignments inferred from hybridization ratios. A threshold of 0.1 was found to be suitable for classifying a gene as absent (i.e., agreement between sequence and CGH data in 99% of the cases), while a cutoff value of 0.3 was conservative for gene presence (agreement in 92% of the cases). Second, we used a set of 65 genes conserved in 26 bacterial genomes, NCBI COG database (http://www.ncbi.nlm.nih.gov/COG/). Genes judged as present in query strains using our selected cut-offs correctly binned data in 1225 out of 1235 total calls. There was a tendency for underprediction of plasmid homologs by CGH, when compared to results from the sequence analysis. Two possible explanations for this are variability in plasmid copy number in B. cereus strains relative to B. anthracis and/or that the average divergence of plasmid genes is greater than chromosomal genes.and that at least two data points were used (exceeded in 99% of the cases)

organism:
Bacillus anthracis
Bacillus mycoides

source_name: Bacillus weihenstephanensis 1143


title: 248_168

source_name: Bacillus cereus strain 248


title: experiment XI, BABE empty vector

description: mammary epithelial cells transduced with BABE empty vector


title: 248_289


title: 1143_342


title: 831_166

source_name: Bacillus cereus strain 831


title: 248_226


title: experiment VIII, BABE empty vector


title: 251_259


title: 10987_246

description:
Additional strain information availiable at www.atcc.org
Amplicons representing 79 of 217 and 41 of 122 genes from pXO1 and pXO2 respectively, and 3601 of 5753 chromosomal genes as predicted by Glimmer 30 ( and see supplemental methods) were arrayed onto glass microscope slides (Telechem, Inc). Redundant genes were generally represented once or a few times on the array.
Genomic DNA was labeled with Cy3 and Cy5 according to J. DeRisi (http://www.microarrays.org/Pdfs/GenomicDNALabel_B.pdf), except that genomic DNA was not digested or sheared before labeling.
Arrays were scanned with a GenePix 4000B scanner (Axon Inc.).
Hybridization signals were quantitated using TIGR SPOTFINDER (software available at http://www.tigr.org/softlab).
Hybridization experiments were competitive using probes derived from B. anthracis Ames (reference) and a B. cereus group (query) strain. Normalized signal intensities were used to generate relative hybridization ratios (query/reference). Data representing weak signal were removed. The ratios from a maximum of six data points (duplicate spots, hybridizations performed in triplicate) were placed in three bins (<0.1) gene is absent in query strain, (0.1-0.3) present but diverged in query strain, and (>0.3) gene is present in the query strain. A majority rule was applied to the data for binning such that more than 50% of ratios were in agreement as to assignment and that at least two data points were used (exceeded in 99% of the cases). In cases where less than two data points existed, the gene was treated as data missing.
The criteria for the numerical ranges of our bins were established in two ways. First, we determined the presence or absence of sequences homologous to 3601 B. anthracis genes in the sequence of B. cereus ATCC 14579 (Integrated Genomics, Inc; http://www.integratedgenomics.com/) using BLASTN and compared that to the assignments inferred from hybridization ratios. A threshold of 0.1 was found to be suitable for classifying a gene as absent (i.e., agreement between sequence and CGH data in 99% of the cases), while a cutoff value of 0.3 was conservative for gene presence (agreement in 92% of the cases). Second, we used a set of 65 genes conserved in 26 bacterial genomes, NCBI COG database (http://www.ncbi.nlm.nih.gov/COG/). Genes judged as present in query strains using our selected cut-offs correctly binned data in 1225 out of 1235 total calls. There was a tendency for underprediction of plasmid homologs by CGH, when compared to results from the sequence analysis. Two possible explanations for this are variability in plasmid copy number in B. cereus strains relative to B. anthracis and/or that the average divergence of plasmid genes is greater than chromosomal genes.and that at least two data points were used (exceeded in 99% of the cases)

source_name: Bacillus cereus ATCC 10987


title: experiment VIII, hTERT


title: 1123_218

description:
additional designation Bacillus cereus 9823; clinical isolate kindly provided by Vincent Ramisse, Institut Pasteur
Amplicons representing 79 of 217 and 41 of 122 genes from pXO1 and pXO2 respectively, and 3601 of 5753 chromosomal genes as predicted by Glimmer 30 ( and see supplemental methods) were arrayed onto glass microscope slides (Telechem, Inc). Redundant genes were generally represented once or a few times on the array.
Genomic DNA was labeled with Cy3 and Cy5 according to J. DeRisi (http://www.microarrays.org/Pdfs/GenomicDNALabel_B.pdf), except that genomic DNA was not digested or sheared before labeling.
Arrays were scanned with a GenePix 4000B scanner (Axon Inc.).
Hybridization signals were quantitated using TIGR SPOTFINDER (software available at http://www.tigr.org/softlab).
Hybridization experiments were competitive using probes derived from B. anthracis Ames (reference) and a B. cereus group (query) strain. Normalized signal intensities were used to generate relative hybridization ratios (query/reference). Data representing weak signal were removed. The ratios from a maximum of six data points (duplicate spots, hybridizations performed in triplicate) were placed in three bins (<0.1) gene is absent in query strain, (0.1-0.3) present but diverged in query strain, and (>0.3) gene is present in the query strain. A majority rule was applied to the data for binning such that more than 50% of ratios were in agreement as to assignment and that at least two data points were used (exceeded in 99% of the cases). In cases where less than two data points existed, the gene was treated as data missing.
The criteria for the numerical ranges of our bins were established in two ways. First, we determined the presence or absence of sequences homologous to 3601 B. anthracis genes in the sequence of B. cereus ATCC 14579 (Integrated Genomics, Inc; http://www.integratedgenomics.com/) using BLASTN and compared that to the assignments inferred from hybridization ratios. A threshold of 0.1 was found to be suitable for classifying a gene as absent (i.e., agreement between sequence and CGH data in 99% of the cases), while a cutoff value of 0.3 was conservative for gene presence (agreement in 92% of the cases). Second, we used a set of 65 genes conserved in 26 bacterial genomes, NCBI COG database (http://www.ncbi.nlm.nih.gov/COG/). Genes judged as present in query strains using our selected cut-offs correctly binned data in 1225 out of 1235 total calls. There was a tendency for underprediction of plasmid homologs by CGH, when compared to results from the sequence analysis. Two possible explanations for this are variability in plasmid copy number in B. cereus strains relative to B. anthracis and/or that the average divergence of plasmid genes is greater than chromosomal genes.and that at least two data points were used (exceeded in 99% of the cases)

source_name: Bacillus cereus strain 1123


title: experiment I, passage 13, replicate 1

description: mammary epithelial cells passage 13


title: experiment I, passage 9, replicate 1

description: mammary epithelial cells passage 9


title: 10987_224


title: 1123_241


title: 1031_172

description:
aka Bacillus thuringiensis 407, kindly provided by Didier Lereclus, Institut Pasteur
Amplicons representing 79 of 217 and 41 of 122 genes from pXO1 and pXO2 respectively, and 3601 of 5753 chromosomal genes as predicted by Glimmer 30 ( and see supplemental methods) were arrayed onto glass microscope slides (Telechem, Inc). Redundant genes were generally represented once or a few times on the array.
Genomic DNA was labeled with Cy3 and Cy5 according to J. DeRisi (http://www.microarrays.org/Pdfs/GenomicDNALabel_B.pdf), except that genomic DNA was not digested or sheared before labeling.
Arrays were scanned with a GenePix 4000B scanner (Axon Inc.).
Hybridization signals were quantitated using TIGR SPOTFINDER (software available at http://www.tigr.org/softlab).
Hybridization experiments were competitive using probes derived from B. anthracis Ames (reference) and a B. cereus group (query) strain. Normalized signal intensities were used to generate relative hybridization ratios (query/reference). Data representing weak signal were removed. The ratios from a maximum of six data points (duplicate spots, hybridizations performed in triplicate) were placed in three bins (<0.1) gene is absent in query strain, (0.1-0.3) present but diverged in query strain, and (>0.3) gene is present in the query strain. A majority rule was applied to the data for binning such that more than 50% of ratios were in agreement as to assignment and that at least two data points were used (exceeded in 99% of the cases). In cases where less than two data points existed, the gene was treated as data missing.
The criteria for the numerical ranges of our bins were established in two ways. First, we determined the presence or absence of sequences homologous to 3601 B. anthracis genes in the sequence of B. cereus ATCC 14579 (Integrated Genomics, Inc; http://www.integratedgenomics.com/) using BLASTN and compared that to the assignments inferred from hybridization ratios. A threshold of 0.1 was found to be suitable for classifying a gene as absent (i.e., agreement between sequence and CGH data in 99% of the cases), while a cutoff value of 0.3 was conservative for gene presence (agreement in 92% of the cases). Second, we used a set of 65 genes conserved in 26 bacterial genomes, NCBI COG database (http://www.ncbi.nlm.nih.gov/COG/). Genes judged as present in query strains using our selected cut-offs correctly binned data in 1225 out of 1235 total calls. There was a tendency for underprediction of plasmid homologs by CGH, when compared to results from the sequence analysis. Two possible explanations for this are variability in plasmid copy number in B. cereus strains relative to B. anthracis and/or that the average divergence of plasmid genes is greater than chromosomal genes.and that at least two data points were used (exceeded in 99% of the cases)

source_name: Bacillus cereus 1031 (Bacillus thuringiensis 407, kindly provided by Didier Lereclus, Institut Pasteur)


title: 259_185


title: experiment IV, hTERT, replicate 2

description: hTERT transduced mammary epithelial cells


title: 1123_167


title: experiment II, hTERT, replicate 2

status: Public on Apr 25 2003


title: 10987_173


title: experiment IV, hTERT transduced, replicate 1


title: experiment IV, BABE transduced, replicate 2

description: BABE empty vector transduced mammary epithelial cells


title: experiment IV, empty vector, replicate 1


title: experiment I hTERT replicate 2

description: ME cells transduced with hTERT


title: experiment II, untransduced, replicate 2

description: mammary epithelial cells


title: experiment II, hTERT, replicate 1


title: experiment II LXSN replicate 1

description: mammary epithelial cells transduced with empty LXSN vector


title: experiment II LXSN replicate 2


title: 1031_233

source_name: Bacillus cereus 1031 (aka Bacillus thuringiensis 407, kindly provided by Didier Lereclus, Institut Pasteur)


title: 1031_279

description:
Bacillus thuringiensis 407, kindly provided by Didier Lereclus, Institut Pasteur
Amplicons representing 79 of 217 and 41 of 122 genes from pXO1 and pXO2 respectively, and 3601 of 5753 chromosomal genes as predicted by Glimmer 30 ( and see supplemental methods) were arrayed onto glass microscope slides (Telechem, Inc). Redundant genes were generally represented once or a few times on the array.
Genomic DNA was labeled with Cy3 and Cy5 according to J. DeRisi (http://www.microarrays.org/Pdfs/GenomicDNALabel_B.pdf), except that genomic DNA was not digested or sheared before labeling.
Arrays were scanned with a GenePix 4000B scanner (Axon Inc.).
Hybridization signals were quantitated using TIGR SPOTFINDER (software available at http://www.tigr.org/softlab).
Hybridization experiments were competitive using probes derived from B. anthracis Ames (reference) and a B. cereus group (query) strain. Normalized signal intensities were used to generate relative hybridization ratios (query/reference). Data representing weak signal were removed. The ratios from a maximum of six data points (duplicate spots, hybridizations performed in triplicate) were placed in three bins (<0.1) gene is absent in query strain, (0.1-0.3) present but diverged in query strain, and (>0.3) gene is present in the query strain. A majority rule was applied to the data for binning such that more than 50% of ratios were in agreement as to assignment and that at least two data points were used (exceeded in 99% of the cases). In cases where less than two data points existed, the gene was treated as data missing.
The criteria for the numerical ranges of our bins were established in two ways. First, we determined the presence or absence of sequences homologous to 3601 B. anthracis genes in the sequence of B. cereus ATCC 14579 (Integrated Genomics, Inc; http://www.integratedgenomics.com/) using BLASTN and compared that to the assignments inferred from hybridization ratios. A threshold of 0.1 was found to be suitable for classifying a gene as absent (i.e., agreement between sequence and CGH data in 99% of the cases), while a cutoff value of 0.3 was conservative for gene presence (agreement in 92% of the cases). Second, we used a set of 65 genes conserved in 26 bacterial genomes, NCBI COG database (http://www.ncbi.nlm.nih.gov/COG/). Genes judged as present in query strains using our selected cut-offs correctly binned data in 1225 out of 1235 total calls. There was a tendency for underprediction of plasmid homologs by CGH, when compared to results from the sequence analysis. Two possible explanations for this are variability in plasmid copy number in B. cereus strains relative to B. anthracis and/or that the average divergence of plasmid genes is greater than chromosomal genes.and that at least two data points were used (exceeded in 99% of the cases)

source_name: Bacillus thuringiensis 1031


title: experiment II untransduced replicate 1

description: mammary epithelial cells


title: experiment I, hTERT, replicate 1


title: experiment I, passage 9, replicate 2

description: untransduced mammary epithelial cells passage 9


title: experiment I, passage 13, replicate 2

description: untransduced mammary epithelial cells passage 13


title: 405_2

status: Public on Apr 22 2003

city: Philadelphia

first: Michael

middle: Patrick

last: Morley

state: PA

address: 3400 Civic Center Blvd, Bldg 421

department: Penn Cardiovascular Institute

institute: Perelman School of Medicine at the University of Pennsylvania

description: Individual LCL vs Pooled Control LCLs

source_name: Individual 405

source_name: Control


title: DBY8778(wt)_stationary_phase_3hours

status: Public on Apr 15 2003

city: Stanford

first: Eran

last: Segal

address: Gates Building 1A

department: Computer Science

institute: Stanford University

description:
Wild type and ypl230w mutant under stationary phase.
DBY8778(wt)_stationary_phase_3hours

organism: Saccharomyces cerevisiae

source_name: reference


title: DBY8778(wt)_stationary_phase_0hours

description:
Wild type and ypl230w mutant under stationary phase.
DBY8778(wt)_stationary_phase_0hours


title: 100W_3_Nov02

status: Public on Apr 19 2003

city: Houston

first: Samuel

last: Kaplan

state: TX

department: Microbiology and Molecular Genetics

institute: University of Texas Health Science Center at Houston

description:
Illumination with tungsten bulbs at 100 W/m2.
Sparged with 95%N/5%CO2.
Harvested at 0.4 OD600.
Boiling lysis/hot phenol RNA extraction.

organism: Cereibacter sphaeroides

source_name: Rhodobacter sphaeroides 2.4.1


title: 10W_2_19Dc02

status: Public on Apr 17 2003

description:
Illumination with lumiline bulbs, 10W/m2
Sparged with 95%N/5%CO2

source_name: R. sphaeroides 2.4.1


title: 10W_1_19Dc02

description:
RNA isolated with boiling lysis/hot phenol
Growth condtions were illumination with lumiline bulbs at 10W/m2.
Sparged with 95%N/5%CO2


title: delKIN82_heat shock_30min

description:
Wild type and kin82 mutant under heat shock.
delKIN82_heat shock_30min

source_name: delKIN82_heat shock_30min


title: DBY8778(wt)_stationary_phase_9hours

description:
Wild type and ypl230w mutant under stationary phase.
DBY8778(wt)_stationary_phase_9hours


title: DBY8778_heat_shock2_30min

description:
Wild type and kin82 mutant under heat shock.
DBY8778_heat_shock2_30min


title: 100W_1_Nov02

description:
Illumination with tungsten bulbs at 100W/m2.
Sparged with 95%N/5%CO2.
Harvested at 0.4 OD600.
Boiling lysis/hot phenol method RNA isolation.


title: DBY8778_heat_shock2_5min

description:
Wild type and kin82 mutant under heat shock.
DBY8778_heat_shock2_5min


title: 100W_2_Nov02

description:
Illumination with tungsten bulbs at 100W/m2.
Sparged with 95%N/5%CO2.
Harvested at 0.4 OD600.
Boiling lysis/hot phenol RNA extraction.


title: 252_1

description: Individual LCL vs Pooled control LCLs

source_name: Individual 252

source_name: control


title: WT_PBS_2

status: Public on Apr 10 2003

city: Parkville

first: Sam

last: Wormald

state: Victoria

address: 1G Royal Parade

department: Cancer and Haematology

country: Australia

institute: The Walter and Eliza Hall Institute

description:
Changes in mouse liver mRNA profiles following intraperitoneal cytokine injection.
One of 2 replicates of albumin-cre(-) Socs3(w/fl) mice, injected with phosphate-buffered saline, and killed after 4h.

source_name: liver


title: DBY8778_heat_shock2_0min

description:
Wild type and kin82 mutant under heat shock.
DBY8778_heat_shock2_0min


title: IFN_PBS_2

description:
Changes in mouse liver mRNA profiles following intra-peritoneal cytokine injection.
One of 2 replicates of Interferon-gamma-/- mice, injected with phosphate-buffered saline, and killed after 4h.


title: S3_IL6_2

description:
Changes in mouse liver mRNA profiles following intraperitoneal cytokine injection.
One of 2 replicates of albumin-cre(+) Socs3(-/fl) mice, injected with interleukin-6, and killed after 4h.


title: S3_PBS_2

description:
Changes in mouse liver mRNA profiles following intraperitoneal cytokine injection.
One of 2 replicates of albumin-cre(+) Socs3(-/fl) mice, injected with phosphate-buffered saline, and killed after 4h.


title: WT_IL6_1

description:
Changes in mouse liver mRNA profiles following intraperitoneal cytokine injection.
One of 2 replicates of albumin-cre(-) Socs3(w/fl) mice, injected with interleukin-6, and killed after 4h.


title: S3_IL6_1


title: IFN_PBS_1


title: WT_IL6_2


title: IFN_IFN_1

description:
Changes in mouse liver mRNA profiles following intra-peritoneal cytokine injection.
One of 2 replicates of Interferon-Gamma-/- mice, injected with Interferon Gamma, and killed after 4h.


title: WT_PBS_1


title: IFN_IFN_2

description:
Changes in mouse liver mRNA profiles following intra-peritoneal cytokine injection.
One of 2 replicates of Interferon-gamma-/- mice, injected with interferon-gamma, and killed after 4h.


title: Spleen vs Untreated B cell 240min (set4)

status: Public on Apr 09 2003

city: Pasadena

first: Sangdun

last: Choi

address: 1200 E. California Blvd

department: Biology

institute: California Institute of Technology

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from untreated mouse (male c57BL/6) B cells cultured for 240min

source_name: Spleen was labeled with Cyanine-3 (green).

source_name: Untreated B cells were labeled with Cyanine-5 (red)


title: S3_PBS_1

description:
Changes in mouse liver mRNA profiles following intra-peritoneal cytokine injection.
One of 2 replicates of albumin-cre(+) Socs3(-/fl) mice, injected with phosphate-buffered saline (PBS), and killed after 4h.


title: Spleen vs Untreated B cell 120min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from untreated mouse (male c57BL/6) B cells cultured for 120min


title: Spleen vs Untreated B cell 240min (set1)


title: Spleen vs Untreated B cell 240min (set2)


title: Spleen vs Untreated B cell 60min (set4)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from untreated mouse (male c57BL/6) B cells cultured for 60min


title: Spleen vs Untreated B cell 120min (set1)


title: Spleen vs Untreated B cell 120min (set2)


title: Spleen vs Untreated B cell 30min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from untreated mouse (male c57BL/6) B cells cultured for 30min


title: Spleen vs Untreated B cell 240min (set3)


title: Spleen vs Untreated B cell Time 0 (set26)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from untreated mouse (male c57BL/6) B cells


title: 1143_330

description:
NOTE: Bacillus weihenstephanensis
Amplicons representing 79 of 217 and 41 of 122 genes from pXO1 and pXO2 respectively, and 3601 of 5753 chromosomal genes as predicted by Glimmer 30 ( and see supplemental methods) were arrayed onto glass microscope slides (Telechem, Inc). Redundant genes were generally represented once or a few times on the array.
Genomic DNA was labeled with Cy3 and Cy5 according to J. DeRisi (http://www.microarrays.org/Pdfs/GenomicDNALabel_B.pdf), except that genomic DNA was not digested or sheared before labeling.
Arrays were scanned with a GenePix 4000B scanner (Axon Inc.).
Hybridization signals were quantitated using TIGR SPOTFINDER (software available at http://www.tigr.org/softlab).
Hybridization experiments were competitive using probes derived from B. anthracis Ames (reference) and a B. cereus group (query) strain. Normalized signal intensities were used to generate relative hybridization ratios (query/reference). Data representing weak signal were removed. The ratios from a maximum of six data points (duplicate spots, hybridizations performed in triplicate) were placed in three bins (<0.1) gene is absent in query strain, (0.1-0.3) present but diverged in query strain, and (>0.3) gene is present in the query strain. A majority rule was applied to the data for binning such that more than 50% of ratios were in agreement as to assignment and that at least two data points were used (exceeded in 99% of the cases). In cases where less than two data points existed, the gene was treated as data missing.
The criteria for the numerical ranges of our bins were established in two ways. First, we determined the presence or absence of sequences homologous to 3601 B. anthracis genes in the sequence of B. cereus ATCC 14579 (Integrated Genomics, Inc; http://www.integratedgenomics.com/) using BLASTN and compared that to the assignments inferred from hybridization ratios. A threshold of 0.1 was found to be suitable for classifying a gene as absent (i.e., agreement between sequence and CGH data in 99% of the cases), while a cutoff value of 0.3 was conservative for gene presence (agreement in 92% of the cases). Second, we used a set of 65 genes conserved in 26 bacterial genomes, NCBI COG database (http://www.ncbi.nlm.nih.gov/COG/). Genes judged as present in query strains using our selected cut-offs correctly binned data in 1225 out of 1235 total calls. There was a tendency for underprediction of plasmid homologs by CGH, when compared to results from the sequence analysis. Two possible explanations for this are variability in plasmid copy number in B. cereus strains relative to B. anthracis and/or that the average divergence of plasmid genes is greater than chromosomal genes.and that at least two data points were used (exceeded in 99% of the cases)

source_name: Bacillus weihenstephanensis 1143

source_name: Bacillus anthracis Ames


title: Spleen vs Untreated B cell Time 0 (set23)


title: Spleen vs Untreated B cell 60min (set3)


title: Spleen vs Untreated B cell 30min (set1)


title: Spleen vs Untreated B cell Time 0 (set27)


title: Spleen vs Untreated B cell Time 0 (set25)


title: Spleen vs Untreated B cell Time 0 (set22)


title: Spleen vs Untreated B cell 60min (set2)


title: Spleen vs Untreated B cell 30min (set3)


title: Spleen vs Untreated B cell 60min (set1)


title: Spleen vs Untreated B cell Time 0 (set21)


title: Spleen vs Untreated B cell Time 0 (set14)


title: Spleen vs Untreated B cell Time 0 (set17)


title: Spleen vs Untreated B cell Time 0 (set16)


title: Spleen vs Untreated B cell Time 0 (set24)


title: Spleen vs Untreated B cell Time 0 (set18)


title: Spleen vs Untreated B cell Time 0 (set19)


title: Spleen vs Untreated B cell Time 0 (set20)


title: Spleen vs Untreated B cell Time 0 (set15)


title: Spleen vs Untreated B cell Time 0 (set13)


title: Spleen vs Untreated B cell Time 0 (set8)


title: Spleen vs Untreated B cell Time 0 (set5)


title: Spleen vs Untreated B cell Time 0 (set7)


title: Spleen vs Untreated B cell Time 0 (set9)


title: Spleen vs Tumor necrosis factor-alpha Treated B cell 120min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Tumor necrosis factor-alpha for 120min

source_name: Tumor necrosis factor-alpha Treated B cells were labeled with Cyanine-5 (red)


title: Spleen vs Untreated B cell Time 0 (set10)


title: Spleen vs Untreated B cell Time 0 (set3)


title: Spleen vs Tumor necrosis factor-alpha Treated B cell 120min (set3)


title: Spleen vs Untreated B cell Time 0 (set2)


title: Spleen vs Untreated B cell Time 0 (set6)


title: Spleen vs Tumor necrosis factor-alpha Treated B cell 30min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Tumor necrosis factor-alpha for 30min


title: Spleen vs Tumor necrosis factor-alpha Treated B cell 60min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Tumor necrosis factor-alpha for 60min


title: Spleen vs Tumor necrosis factor-alpha Treated B cell 30min (set1)


title: Spleen vs Untreated B cell Time 0 (set12)


title: Spleen vs TGF-beta (transforming growth factor-beta) Treated B cell 60min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with TGF-beta (transforming growth factor-beta) for 60min
Lot batch =

source_name: TGF-beta (transforming growth factor-beta) Treated B cells were labeled with Cyanine-5 (red)


title: Spleen vs TGF-beta Treated B cell 240min (set1)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with TGF-beta (transforming growth factor-beta) for 240min


title: Spleen vs Tumor necrosis factor-alpha Treated B cell 240min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Tumor necrosis factor-alpha for 240min


title: Spleen vs TGF-beta (transforming growth factor-beta) Treated B cell 60min (set1)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with TGF-beta (transforming growth factor-beta) for 60min


title: Spleen vs Untreated B cell Time 0 (set4)


title: Spleen vs Tumor necrosis factor-alpha Treated B cell 120min (set1)


title: Spleen vs TGF-beta Treated B cell 120min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with TGF-beta (transforming growth factor-beta) for 120min


title: Spleen vs Tumor necrosis factor-alpha Treated B cell 60min (set3)


title: Spleen vs Tumor necrosis factor-alpha Treated B cell 60min (set1)


title: Spleen vs TGF-beta (transforming growth factor-beta) Treated B cell 30min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with TGF-beta (transforming growth factor-beta) for 30min


title: Spleen vs Tumor necrosis factor-alpha Treated B cell 240min (set1)


title: Spleen vs TGF-beta (transforming growth factor-beta) Treated B cell 30min (set2)


title: Spleen vs Tumor necrosis factor-alpha Treated B cell 30min (set3)


title: Spleen vs Untreated B cell Time 0 (set11)


title: Spleen vs TGF-beta Treated B cell 240min (set2)


title: Spleen vs Untreated B cell Time 0 (set1)


title: Spleen vs TGF-beta (transforming growth factor-beta) Treated B cell 30min (set1)


title: Spleen vs Terbutaline Treated B cell 240min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated withTerbutaline for 240min

source_name: Terbutaline Treated B cells were labeled with Cyanine-5 (red)


title: Spleen vs Terbutaline Treated B cell 240min (set1)


title: 0 hr

city: East Lansing

first: Tim

last: Zacharewski

state: MI

address: 223 Biochemistry Bldg.

department: Biochemistry & Molecular Biology, National Food Safety & Toxicol

institute: Michigan State University

description:
Immature, ovariectomized C57BL/6 mice were treated with sesame oil (vehicle) or with 0.1mg/kg 17-alpha-ethynylestradiol (EE) for the length of time indicated. Mice received one dose, except for the 3x24hr samples, which received 3 consecutive daily doses and were sacrificed 24hr after the final dose.
Keywords = estrogen, uterus, mouse, ovariectomized, gavage

source_name: whole uterus


title: Spleen vs Terbutaline Treated B cell 60min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated withTerbutaline for 60min


title: Spleen vs Terbutaline Treated B cell 120min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated withTerbutaline for 120min


title: Spleen vs Terbutaline Treated B cell 240min (set2)


title: 3x24 hr EE repl 2


title: 24 hr EE repl 1


title: 3x24 hr EE repl 1


title: 8 hr EE repl 1


title: 8 hr EE repl 2


title: 2 hr EE repl 2


title: 24 hr EE repl 2


title: 12 hr EE repl 1


title: Spleen vs SLC (Secondary lymphoid-organ chemokine) Treated B cell 240min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated withSLC (Secondary lymphoid-organ chemokine) for 240min

source_name: SLC (Secondary lymphoid-organ chemokine) Treated B cells were labeled with Cyanine-5 (red)


title: Spleen vs Terbutaline Treated B cell 30min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated withTerbutaline for 30min


title: Spleen vs SLC (Secondary lymphoid-organ chemokine) Treated B cell 120min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated withSLC (Secondary lymphoid-organ chemokine) for 120min


title: 2 hr EE repl 1


title: 12 hr EE repl 2


title: 3x24 hr vehicle repl 1


title: 3x24 hr vehicle repl 2


title: Spleen vs Terbutaline Treated B cell 30min (set1)


title: 24 hr vehicle repl 2


title: 24 hr vehicle repl 1


title: Spleen vs SLC (Secondary lymphoid-organ chemokine) Treated B cell 240min (set3)


title: Spleen vs SLC (Secondary lymphoid-organ chemokine) Treated B cell 120min (set1)


title: Spleen vs SLC (Secondary lymphoid-organ chemokine) Treated B cell 120min (set2)


title: 12 hr vehicle repl 1


title: Spleen vs Terbutaline Treated B cell 120min (set1)


title: Spleen vs SLC (Secondary lymphoid-organ chemokine) Treated B cell 60min (set1)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated withSLC (Secondary lymphoid-organ chemokine) for 60min


title: Spleen vs SLC (Secondary lymphoid-organ chemokine) Treated B cell 60min (set2)


title: Spleen vs SLC (Secondary lymphoid-organ chemokine) Treated B cell 30min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated withSLC (Secondary lymphoid-organ chemokine) for 30min


title: Spleen vs Terbutaline Treated B cell 60min (set1)


title: Spleen vs SLC (Secondary lymphoid-organ chemokine) Treated B cell 60min (set3)


title: Spleen vs SLC (Secondary lymphoid-organ chemokine) Treated B cell 240min (set1)


title: 12 hr vehicle repl 2


title: Spleen vs SLC (Secondary lymphoid-organ chemokine) Treated B cell 30min (set3)


title: Spleen vs SDF1 alpha Treated B cell 240min (set1)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with SDF1 alpha (Stromal cell derived factor-1) for 240min

source_name: SDF1 alpha (Stromal cell derived factor-1) Treated B cells were labeled with Cyanine-5 (red)


title: Spleen vs SLC (Secondary lymphoid-organ chemokine) Treated B cell 30min (set1)


title: Spleen vs SDF1 alpha Treated B cell 240min (set3)


title: Spleen vs Terbutaline Treated B cell 120min (set3)


title: Spleen vs Terbutaline Treated B cell 60min (set2)


title: Spleen vs SDF1 alpha Treated B cell 240min (set2)


title: 8 hr vehicle repl 2


title: 2 hr vehicle repl 2


title: Spleen vs Terbutaline Treated B cell 30min (set3)


title: 8 hr vehicle repl 1


title: Spleen vs SDF1 alpha Treated B cell 120min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with SDF1 alpha (Stromal cell derived factor-1) for 120min


title: Spleen vs SDF1 alpha Treated B cell 120min (set2)


title: 2 hr vehicle repl 1


title: Spleen vs TGF-beta Treated B cell 120min (set3)


title: Spleen vs SDF1 alpha (Stromal cell derived factor-1) Treated B cell 30min (set3)

status: Public on Apr 05 2003

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with SDF1 alpha (Stromal cell derived factor-1) for 30min


title: Spleen vs SDF1 alpha (Stromal cell derived factor-1) Treated B cell 60min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with SDF1 alpha (Stromal cell derived factor-1) for 60min


title: Spleen vs SDF1 alpha Treated B cell 120min (set1)


title: Spleen vs Sphingosine-1-phosphate Treated B cell 240min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Sphingosine-1-phosphate for 240min

source_name: Sphingosine-1-phosphate Treated B cells were labeled with Cyanine-5 (red)


title: Spleen vs SDF1 alpha (Stromal cell derived factor-1) Treated B cell 30min (set2)


title: Spleen vs Sphingosine-1-phosphate Treated B cell 120min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Sphingosine-1-phosphate for 120min


title: Spleen vs Sphingosine-1-phosphate Treated B cell 120min (set1)


title: Spleen vs Sphingosine-1-phosphate Treated B cell 240min (set1)


title: Spleen vs SDF1 alpha (Stromal cell derived factor-1) Treated B cell 30min (set1)


title: Spleen vs Sphingosine-1-phosphate Treated B cell 120min (set3)


title: Spleen vs Sphingosine-1-phosphate Treated B cell 240min (set2)


title: Spleen vs Sphingosine-1-phosphate Treated B cell 60min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Sphingosine-1-phosphate for 60min


title: Spleen vs SDF1 alpha (Stromal cell derived factor-1) Treated B cell 60min (set1)


title: Spleen vs SDF1 alpha (Stromal cell derived factor-1) Treated B cell 60min (set2)


title: Spleen vs Prostaglandin E2 Treated B cell 240min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Prostaglandin E2 for 240min

source_name: Prostaglandin E2 Treated B cells were labeled with Cyanine-5 (red)


title: Spleen vs Sphingosine-1-phosphate Treated B cell 30min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Sphingosine-1-phosphate for 30min


title: Spleen vs Sphingosine-1-phosphate Treated B cell 30min (set1)


title: Spleen vs Prostaglandin E2 Treated B cell 240min (set3)


title: Spleen vs Prostaglandin E2 Treated B cell 60min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Prostaglandin E2 for 60min


title: Spleen vs Sphingosine-1-phosphate Treated B cell 60min (set3)


title: Spleen vs Sphingosine-1-phosphate Treated B cell 30min (set3)


title: Spleen vs Prostaglandin E2 Treated B cell 120min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Prostaglandin E2 for 120min


title: Spleen vs Prostaglandin E2 Treated B cell 60min (set1)


title: Spleen vs Prostaglandin E2 Treated B cell 30min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Prostaglandin E2 for 30min


title: Spleen vs Sphingosine-1-phosphate Treated B cell 60min (set1)


title: Spleen vs Prostaglandin E2 Treated B cell 60min (set2)


title: Spleen vs Prostaglandin E2 Treated B cell 120min (set3)


title: Spleen vs Prostaglandin E2 Treated B cell 120min (set1)


title: Spleen vs Prostaglandin E2 Treated B cell 30min (set3)


title: Spleen vs Prostaglandin E2 Treated B cell 240min (set1)


title: Spleen vs Prostaglandin E2 Treated B cell 30min (set1)


title: Spleen vs Platelet activating factor Treated B cell 240min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Platelet activating factor for 240min

source_name: Platelet activating factor Treated B cells were labeled with Cyanine-5 (red)


title: Spleen vs Platelet activating factor Treated B cell 30min (set1)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Platelet activating factor for 30min


title: Spleen vs Neuropeptide Y Treated B cell 120min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Neuropeptide Y for 120min

source_name: Neuropeptide Y Treated B cells were labeled with Cyanine-5 (red)


title: Spleen vs Platelet activating factor Treated B cell 60min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Platelet activating factor for 60min


title: Spleen vs Platelet activating factor Treated B cell 30min (set3)


title: Spleen vs Neuropeptide Y Treated B cell 60min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Neuropeptide Y for 60min


title: Spleen vs Platelet activating factor Treated B cell 240min (set1)


title: Spleen vs NGF (Nerve Growth Factor)Treated B cell 60min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with NGF (Nerve Growth Factor) for 60min

source_name: NGF (Nerve Growth Factor) Treated B cells were labeled with Cyanine-5 (red)


title: Spleen vs Neuropeptide Y Treated B cell 120min (set3)


title: Spleen vs Platelet activating factor Treated B cell 120min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Platelet activating factor for 120min


title: Spleen vs Neuropeptide Y Treated B cell 240min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Neuropeptide Y for 240min


title: Spleen vs Neuropeptide Y Treated B cell 30min (set1)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Neuropeptide Y for 30min


title: Spleen vs Neuropeptide Y Treated B cell 120min (set1)


title: Spleen vs Platelet activating factor Treated B cell 60min (set1)


title: Spleen vs NGF (Nerve Growth Factor)Treated B cell 120min (set1)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with NGF (Nerve Growth Factor) for 120min


title: Spleen vs Platelet activating factor Treated B cell 240min (set2)


title: Spleen vs Neuropeptide Y Treated B cell 30min (set2)


title: Spleen vs NGF (Nerve Growth Factor)Treated B cell 240min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with NGF (Nerve Growth Factor) for 240min


title: Spleen vs Platelet activating factor Treated B cell 30min (set2)


title: Spleen vs Neuropeptide Y Treated B cell 60min (set3)


title: Spleen vs NGF (Nerve Growth Factor)Treated B cell 240min (set3)


title: Spleen vs Neuropeptide Y Treated B cell 240min (set1)


title: Spleen vs NGF (Nerve Growth Factor)Treated B cell 240min (set1)


title: Spleen vs Neuropeptide Y Treated B cell 60min (set1)


title: Spleen vs NGF (Nerve Growth Factor)Treated B cell 120min (set2)


title: Spleen vs Platelet activating factor Treated B cell 60min (set3)


title: Spleen vs NGF (Nerve Growth Factor)Treated B cell 60min (set2)


title: Spleen vs Neuropeptide Y Treated B cell 30min (set3)


title: Spleen vs NGF (Nerve Growth Factor)Treated B cell 120min (set3)


title: Spleen vs NGF (Nerve Growth Factor)Treated B cell 30min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with NGF (Nerve Growth Factor) for 30min


title: Spleen vs MIP3-alpha Treated B cell 120min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with MIP3-alpha (Macrophage inflammatory protein-3) for 120min

source_name: MIP3-alpha (Macrophage inflammatory protein-3) Treated B cells were labeled with Cyanine-5 (red)


title: Spleen vs MIP3-alpha Treated B cell 240min (set1)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with MIP3-alpha (Macrophage inflammatory protein-3) for 240min


title: Spleen vs MIP3-alpha Treated B cell 240min (set3)


title: Spleen vs MIP3-alpha Treated B cell 60min (set1)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with MIP3-alpha (Macrophage inflammatory protein-3) for 60min


title: Spleen vs NGF (Nerve Growth Factor)Treated B cell 30min (set2)


title: Spleen vs MIP3-alpha Treated B cell 30min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with MIP3-alpha (Macrophage inflammatory protein-3) for 30min


title: Spleen vs Leukotriene B4 (LTB4)Treated B cell 240min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Leukotriene B4 (LTB4) for 240min

source_name: Leukotriene B4 (LTB4) Treated B cells were labeled with Cyanine-5 (red)


title: Spleen vs Leukotriene B4 (LTB4)Treated B cell 240min (set2)

source_name: Spleen was labeled with Cyanine-3 (green).


title: Spleen vs MIP3-alpha Treated B cell 30min (set2)


title: Spleen vs MIP3-alpha Treated B cell 120min (set3)


title: Spleen vs MIP3-alpha Treated B cell 120min (set1)


title: Spleen vs Leukotriene B4 (LTB4)Treated B cell 240min (set1)


title: Spleen vs Leukotriene B4 (LTB4)Treated B cell 120min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Leukotriene B4 (LTB4) for 120min


title: Spleen vs NGF (Nerve Growth Factor)Treated B cell 30min (set1)


title: Spleen vs NGF (Nerve Growth Factor)Treated B cell 60min (set1)


title: Spleen vs MIP3-alpha Treated B cell 60min (set3)


title: Spleen vs MIP3-alpha Treated B cell 30min (set1)


title: Spleen vs Leukotriene B4 (LTB4)Treated B cell 60min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Leukotriene B4 (LTB4) for 60min


title: Spleen vs MIP3-alpha Treated B cell 60min (set2)


title: Spleen vs Leukotriene B4 (LTB4)Treated B cell 60min (set3)


title: Spleen vs Leukotriene B4 (LTB4)Treated B cell 30min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Leukotriene B4 (LTB4) for 30min


title: Spleen vs Leukotriene B4 (LTB4)Treated B cell 30min (set2)


title: Spleen vs MIP3-alpha Treated B cell 240min (set2)


title: Spleen vs Leukotriene B4 (LTB4)Treated B cell 120min (set1)


title: Spleen vs Lipopolysaccharide (LPS) Treated B cell 240min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Lipopolysaccharide (LPS) for 240min

source_name: Lipopolysaccharide (LPS) Treated B cells were labeled with Cyanine-5 (red)


title: Spleen vs Leukotriene B4 (LTB4)Treated B cell 60min (set1)


title: Spleen vs Leukotriene B4 (LTB4)Treated B cell 30min (set1)


title: Spleen vs Lipopolysaccharide (LPS) Treated B cell 120min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Lipopolysaccharide (LPS) for 120min


title: Spleen vs Lipopolysaccharide (LPS) Treated B cell 240min (set1)


title: Spleen vs Lipopolysaccharide (LPS) Treated B cell 60min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Lipopolysaccharide (LPS) for 60min


title: Spleen vs Lipopolysaccharide (LPS) Treated B cell 30min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Lipopolysaccharide (LPS) for 30min


title: Spleen vs Lipopolysaccharide (LPS) Treated B cell 30min (set1)


title: Spleen vs Lipopolysaccharide (LPS) Treated B cell 120min (set2)


title: Spleen vs Lipopolysaccharide (LPS) Treated B cell 60min (set2)


title: Spleen vs Lysophosphatidic Acid Treated B cell 60min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Lysophosphatidic Acid for 60min

source_name: Lysophosphatidic Acid Treated B cells were labeled with Cyanine-5 (red)


title: Spleen vs Lipopolysaccharide (LPS) Treated B cell 30min (set2)


title: Spleen vs Lysophosphatidic Acid Treated B cell 60min (set1)


title: Spleen vs Lysophosphatidic Acid Treated B cell 240min (set1)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Lysophosphatidic Acid for 240min


title: Spleen vs Lipopolysaccharide (LPS) Treated B cell 60min (set1)


title: Spleen vs Lipopolysaccharide (LPS) Treated B cell 240min (set2)


title: Spleen vs Lysophosphatidic Acid Treated B cell 120min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Lysophosphatidic Acid for 120min


title: Spleen vs Lipopolysaccharide (LPS) Treated B cell 120min (set1)


title: Spleen vs Lysophosphatidic Acid Treated B cell 120min (set1)


title: Spleen vs IL10 Treated B cell 240min (set1)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with IL10 for 240min

source_name: IL10 Treated B cells were labeled with Cyanine-5 (red)


title: Spleen vs Lysophosphatidic Acid Treated B cell 30min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Lysophosphatidic Acid for 30min


title: Spleen vs Lysophosphatidic Acid Treated B cell 240min (set3)


title: Spleen vs IL10 Treated B cell 240min (set2)


title: Spleen vs IL10 Treated B cell 120min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with IL10 for 120min


title: Spleen vs IL10 Treated B cell 120min (set2)


title: Spleen vs Lysophosphatidic Acid Treated B cell 30min (set2)


title: Spleen vs Lysophosphatidic Acid Treated B cell 60min (set2)


title: Spleen vs IL4 Treated B cell 240min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with IL4 for 240min

source_name: IL4 Treated B cells were labeled with Cyanine-5 (red)


title: Spleen vs Lysophosphatidic Acid Treated B cell 120min (set3)


title: Spleen vs Lysophosphatidic Acid Treated B cell 30min (set1)


title: Spleen vs IL10 Treated B cell 120min (set1)


title: Spleen vs IL10 Treated B cell 240min (set3)


title: Spleen vs IL4 Treated B cell 240min (set1)


title: Spleen vs IL4 Treated B cell 120min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with IL4 for 120min


title: Spleen vs IL4 Treated B cell 240min (set2)


title: Spleen vs IL4 Treated B cell 120min (set3)


title: Spleen vs IL4 Treated B cell 60min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with IL4 for 60min


title: Spleen vs IL4 Treated B cell 60min (set2)


title: Spleen vs Insulin-like growth factor 1 Treated B cell 240min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Insulin-like growth factor 1 for 240min

source_name: Insulin-like growth factor 1 Treated B cells were labeled with Cyanine-5 (red)


title: Spleen vs IL10 Treated B cell 60min (set1)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with IL10 for 60min


title: Spleen vs IL10 Treated B cell 60min (set3)


title: Spleen vs IL4 Treated B cell 30min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with IL4 for 30min


title: Spleen vs IL10 Treated B cell 30min (set1)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with IL10 for 30min


title: Spleen vs IL4 Treated B cell 30min (set3)


title: Spleen vs Insulin-like growth factor 1 Treated B cell 240min (set2)


title: Spleen vs Leukotriene B4 (LTB4)Treated B cell 120min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Leukotriene B4 (LTB4) for 120min


title: Spleen vs Insulin-like growth factor 1 Treated B cell 60min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Insulin-like growth factor 1 for 60min


title: Spleen vs IL10 Treated B cell 60min (set2)


title: Spleen vs Insulin-like growth factor 1 Treated B cell 30min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Insulin-like growth factor 1 for 30min


title: Spleen vs Insulin-like growth factor 1 Treated B cell 240min (set1)


title: Spleen vs IL4 Treated B cell 30min (set1)


title: Spleen vs Insulin-like growth factor 1 Treated B cell 60min (set2)


title: Spleen vs Insulin-like growth factor 1 Treated B cell 60min (set1)


title: Spleen vs Insulin-like growth factor 1 Treated B cell 120min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Insulin-like growth factor 1 for 120min


title: Spleen vs Insulin-like growth factor 1 Treated B cell 120min (set1)


title: Spleen vs IL10 Treated B cell 30min (set2)


title: Spleen vs IL4 Treated B cell 120min (set1)


title: Spleen vs Insulin-like growth factor 1 Treated B cell 30min (set2)


title: Spleen vs Insulin-like growth factor 1 Treated B cell 120min (set2)


title: Spleen vs Insulin-like growth factor 1 Treated B cell 30min (set1)


title: Spleen vs Interferon-gamma (IFN gamma) Treated B cell 120min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Interferon-gamma (IFN gamma) for 120min

source_name: Interferon-gamma (IFN gamma) Treated B cells were labeled with Cyanine-5 (red)


title: Spleen vs Interferon-gamma (IFN gamma) Treated B cell 240min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Interferon-gamma (IFN gamma) for 240min


title: Spleen vs Interferon-gamma (IFN gamma) Treated B cell 120min (set3)


title: Spleen vs Interferon-gamma (IFN gamma) Treated B cell 120min (set1)


title: Spleen vs Interferon-gamma (IFN gamma) Treated B cell 60min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Interferon-gamma (IFN gamma) for 60min


title: Spleen vs Interferon-beta (IFN beta) Treated B cell 60min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Interferon-beta (IFN beta) for 60min

source_name: Interferon-beta (IFN beta) Treated B cells were labeled with Cyanine-5 (red)


title: Spleen vs Interferon-gamma (IFN gamma) Treated B cell 60min (set2)


title: Spleen vs Interferon-beta (IFN beta) Treated B cell 120min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Interferon-beta (IFN beta) for 120min


title: Spleen vs Interferon-beta (IFN beta) Treated B cell 240min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Interferon-beta (IFN beta) for 240min


title: Spleen vs Interferon-gamma (IFN gamma) Treated B cell 30min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Interferon-gamma (IFN gamma) for 30min


title: Spleen vs Interferon-beta (IFN beta) Treated B cell 240min (set3)


title: Spleen vs Interferon-beta (IFN beta) Treated B cell 240min (set1)


title: Spleen vs Interferon-gamma (IFN gamma) Treated B cell 30min (set3)


title: Spleen vs Interferon-beta (IFN beta) Treated B cell 60min (set3)


title: Spleen vs Interferon-beta (IFN beta) Treated B cell 30min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Interferon-beta (IFN beta) for 30min


title: Spleen vs Interferon-gamma (IFN gamma) Treated B cell 60min (set1)


title: Spleen vs Interferon-beta (IFN beta) Treated B cell 120min (set2)


title: Spleen vs Interferon-gamma (IFN gamma) Treated B cell 30min (set1)


title: Spleen vs Interferon-beta (IFN beta) Treated B cell 30min (set1)


title: Spleen vs Interferon-beta (IFN beta) Treated B cell 30min (set2)


title: Spleen vs Growth Hormone Release Hormone (GHRH) Treated B cell 240min (set1)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Growth Hormone Release Hormone (GHRH) for 240min

source_name: Growth Hormone Release Hormone (GHRH) Treated B cells were labeled with Cyanine-5 (red)


title: Spleen vs Growth Hormone Release Hormone (GHRH) Treated B cell 240min (set3)


title: Spleen vs Lysophosphatidic Acid Treated B cell 240min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Lysophosphatidic Acid for 240min


title: Spleen vs Growth Hormone Release Hormone (GHRH) Treated B cell 120min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Growth Hormone Release Hormone (GHRH) for 120min


title: Spleen vs Growth Hormone Release Hormone (GHRH) Treated B cell 240min (set2)


title: Spleen vs Interferon-beta (IFN beta) Treated B cell 120min (set1)


title: Spleen vs Interferon-beta (IFN beta) Treated B cell 60min (set1)


title: Spleen vs Growth Hormone Release Hormone (GHRH) Treated B cell 120min (set1)


title: Spleen vs Growth Hormone Release Hormone (GHRH) Treated B cell 120min (set2)


title: Spleen vs Growth Hormone Release Hormone (GHRH) Treated B cell 60min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Growth Hormone Release Hormone (GHRH) for 60min


title: Spleen vs fMLP Treated B cell 240min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with fMLP for 240min

source_name: fMLP Treated B cells were labeled with Cyanine-5 (red)


title: Spleen vs Growth Hormone Release Hormone (GHRH) Treated B cell 30min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Growth Hormone Release Hormone (GHRH) for 30min


title: Spleen vs Growth Hormone Release Hormone (GHRH) Treated B cell 60min (set1)


title: Spleen vs Interferon-gamma (IFN gamma) Treated B cell 240min (set2)


title: Spleen vs fMLP Treated B cell 60min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with fMLP for 60min


title: Spleen vs fMLP Treated B cell 240min (set2)


title: Spleen vs Growth Hormone Release Hormone (GHRH) Treated B cell 60min (set2)


title: Spleen vs fMLP Treated B cell 30min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with fMLP for 30min


title: Spleen vs fMLP Treated B cell 30min (set1)


title: Spleen vs Growth Hormone Release Hormone (GHRH) Treated B cell 30min (set2)


title: Spleen vs ELC Treated B cell 120min (set1)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with ELC for 120min

source_name: ELC Treated B cells were labeled with Cyanine-5 (red)


title: Spleen vs Growth Hormone Release Hormone (GHRH) Treated B cell 30min (set1)


title: Spleen vs fMLP Treated B cell 60min (set2)


title: Spleen vs fMLP Treated B cell 120min (set1)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with fMLP for 120min


title: Spleen vs ELC Treated B cell 120min (set3)


title: Spleen vs ELC Treated B cell 240min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with ELC for 240min


title: Spleen vs ELC Treated B cell 60min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with ELC for 60min


title: Spleen vs fMLP Treated B cell 30min (set3)


title: Spleen vs ELC Treated B cell 120min (set2)


title: Spleen vs ELC Treated B cell 60min (set1)


title: Spleen vs fMLP Treated B cell 240min (set1)


title: Spleen vs fMLP Treated B cell 60min (set1)


title: Spleen vs IL4 Treated B cell 60min (set1)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with IL4 for 60min


title: Spleen vs ELC Treated B cell 240min (set1)


title: Spleen vs ELC Treated B cell 60min (set2)


title: Spleen vs ELC Treated B cell 30min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with ELC for 30min


title: Spleen vs fMLP Treated B cell 120min (set2)


title: Spleen vs ELC Treated B cell 30min (set3)


title: Spleen vs Interferon-gamma (IFN gamma) Treated B cell 240min (set1)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Interferon-gamma (IFN gamma) for 240min


title: Spleen vs ELC Treated B cell 30min (set1)


title: Spleen vs Dimaprit Treated B cell 120min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Dimaprit for 120min

source_name: Dimaprit Treated B cells were labeled with Cyanine-5 (red)


title: Spleen vs CpG-oligodeoxynucleotide Treated B cell 60min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with CpG-oligodeoxynucleotide for 60min

source_name: CpG-oligodeoxynucleotide Treated B cells were labeled with Cyanine-5 (red)


title: Spleen vs Dimaprit Treated B cell 240min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Dimaprit for 240min


title: Spleen vs CpG-oligodeoxynucleotide Treated B cell 120min (set1)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with CpG-oligodeoxynucleotide for 120min


title: Spleen vs CpG-oligodeoxynucleotide Treated B cell 240min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with CpG-oligodeoxynucleotide for 240min


title: Spleen vs CpG-oligodeoxynucleotide Treated B cell 60min (set1)


title: Spleen vs CpG-oligodeoxynucleotide Treated B cell 30min (set1)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with CpG-oligodeoxynucleotide for 30min


title: Spleen vs CpG-oligodeoxynucleotide Treated B cell 120min (set3)


title: Spleen vs CGS Treated B cell 240min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with CGS for 240min

source_name: CGS Treated B cells were labeled with Cyanine-5 (red)


title: Spleen vs Dimaprit Treated B cell 240min (set2)


title: Spleen vs CpG-oligodeoxynucleotide Treated B cell 240min (set3)


title: Spleen vs Dimaprit Treated B cell 30min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Dimaprit for 30min


title: Spleen vs Dimaprit Treated B cell 30min (set1)


title: Spleen vs CpG-oligodeoxynucleotide Treated B cell 120min (set2)


title: Spleen vs Dimaprit Treated B cell 60min (set1)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Dimaprit for 60min


title: Spleen vs CpG-oligodeoxynucleotide Treated B cell 30min (set2)


title: Spleen vs Dimaprit Treated B cell 60min (set3)


title: Spleen vs Dimaprit Treated B cell 60min (set2)


title: Spleen vs CGS Treated B cell 240min (set1)


title: Spleen vs CpG-oligodeoxynucleotide Treated B cell 30min (set3)


title: Spleen vs CpG-oligodeoxynucleotide Treated B cell 240min (set1)


title: Spleen vs Dimaprit Treated B cell 30min (set3)


title: Spleen vs Dimaprit Treated B cell 240min (set1)


title: Spleen vs Dimaprit Treated B cell 120min (set2)


title: Spleen vs CpG-oligodeoxynucleotide Treated B cell 60min (set3)


title: Spleen vs CGS Treated B cell 240min (set2)


title: Spleen vs CGS Treated B cell 120min (set1)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with CGS for 120min


title: Spleen vs CGS Treated B cell 30min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with CGS for 30min


title: Spleen vs CGS Treated B cell 60min (set1)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with CGS for 60min


title: Spleen vs CGS Treated B cell 60min (set3)


title: Spleen vs CGS Treated B cell 120min (set2)


title: Spleen vs CD40 Treated B cell 240min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with CD40 for 240min

source_name: CD40 Treated B cells were labeled with Cyanine-5 (red)


title: Spleen vs CD40 Treated B cell 120min (set2)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with CD40 for 120min


title: Spleen vs CD27 Treated B cell 120min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with CD27 for 120min

source_name: CD27 Treated B cells were labeled with Cyanine-5 (red)


title: Spleen vs CGS Treated B cell 30min (set1)


title: Spleen vs CGS Treated B cell 30min (set3)


title: Spleen vs CD27 Treated B cell 240min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with CD27 for 240min


title: Spleen vs CD27 Treated B cell 120min (set1)


title: Spleen vs CD40 Treated B cell 120min (set1)


title: Spleen vs CD27 Treated B cell 240min (set2)


title: Spleen vs CD40 Treated B cell 120min (set3)


title: Spleen vs CD40 Treated B cell 60min (set3)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with CD40 for 60min


title: Spleen vs CD40 Treated B cell 30min (set1)

description:
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with CD40 for 30min


title: Spleen vs CD40 Treated B cell 60min (set2)


title: Spleen vs CGS Treated B cell 60min (set2)


title: Spleen vs CD40 Treated B cell 240min (set1)


title: Spleen vs CD27 Treated B cell 120min (set2)