title: Single SP cell sample H (SCH)

status: Public on Jan 02 2006

city: Houston

first: Carlos

middle: Almeida

last: Ramos

state: TX

address: One Baylor Plaza

department: Center for Cell and Gene Therapy

institute: Baylor College of Medicine

description:
Single SP cells from C57Bl/6 male mouse bone marrow, Sca-1 positive and Gr-1 negative, gated on the tip of the SP tail, were sorted into individual wells of 96-well plates. The mRNA of each cell was amplified by the global single cell RT-PCR method and biotinylated targets were generated after optimal digestion with DNAse I. Detection of the microarray hybridization signals was done according to the standard Affymetrix protocol (antibody amplified).
This sample is also known as HSH
Keywords = SP, side population, global single cell RT-PCR, GSC RT-PCR, microarray, HSC, stem cell

source_name: Bone marrow, tip of side population, Sca-1 positive, Gr-1 negative cell


title: Single SP cell equivalent sample D (SCED)

type: RNA

country: USA

description:
Forty bone marrow SP cells from C57Bl/6 male mouse bone marrow, Sca-1 positive and Gr-1 negative, gated on the tip of the SP tail, were sorted into 160 microliters of lysis buffer (40 times the amount used for single cells). 4-microliter aliquots (containing the mRNA equivalent to one single cell) were dispensed into individual wells of 96-well plates. The mRNA contained in each aliquot was amplified by the global single cell RT-PCR method and biotinylated targets were generated after optimal digestion with DNAse I. Detection of the microarray hybridization signals was done according to the standard Affymetrix protocol (antibody amplified).
This sample is also known as HWD
Keywords = SP, side population, global single cell RT-PCR, GSC RT-PCR, microarray, HSC, stem cell

molecule: total RNA

organism: Mus musculus

source_name: Bone marrow, tip of side population, Sca-1 positive, Gr-1 negative cells


title: Single SP cell equivalent sample C (SCEC)

description:
Forty bone marrow SP cells from C57Bl/6 male mouse bone marrow, Sca-1 positive and Gr-1 negative, gated on the tip of the SP tail, were sorted into 160 microliters of lysis buffer (40 times the amount used for single cells). 4-microliter aliquots (containing the mRNA equivalent to one single cell) were dispensed into individual wells of 96-well plates. The mRNA contained in each aliquot was amplified by the global single cell RT-PCR method and biotinylated targets were generated after optimal digestion with DNAse I. Detection of the microarray hybridization signals was done according to the standard Affymetrix protocol (antibody amplified).
This sample is also known as HWC
Keywords = SP, side population, global single cell RT-PCR, GSC RT-PCR, microarray, HSC, stem cell


title: Single SP cell equivalent sample B (SCEB)

description:
Forty bone marrow SP cells from C57Bl/6 male mouse bone marrow, Sca-1 positive and Gr-1 negative, gated on the tip of the SP tail, were sorted into 160 microliters of lysis buffer (40 times the amount used for single cells). 4-microliter aliquots (containing the mRNA equivalent to one single cell) were dispensed into individual wells of 96-well plates. The mRNA contained in each aliquot was amplified by the global single cell RT-PCR method and biotinylated targets were generated after optimal digestion with DNAse I. Detection of the microarray hybridization signals was done according to the standard Affymetrix protocol (antibody amplified).
This sample is also known as HWB
Keywords = SP, side population, global single cell RT-PCR, GSC RT-PCR, microarray, HSC, stem cell


title: Single SP cell equivalent sample E (SCEE)

description:
Forty bone marrow SP cells from C57Bl/6 male mouse bone marrow, Sca-1 positive and Gr-1 negative, gated on the tip of the SP tail, were sorted into 160 microliters of lysis buffer (40 times the amount used for single cells). 4-microliter aliquots (containing the mRNA equivalent to one single cell) were dispensed into individual wells of 96-well plates. The mRNA contained in each aliquot was amplified by the global single cell RT-PCR method and biotinylated targets were generated after optimal digestion with DNAse I. Detection of the microarray hybridization signals was done according to the standard Affymetrix protocol (antibody amplified).
This sample is also known as HWE
Keywords = SP, side population, global single cell RT-PCR, GSC RT-PCR, microarray, HSC, stem cell


title: Single SP cell sample L (SCL)

description:
Single SP cells from C57Bl/6 male mouse bone marrow, Sca-1 positive and Gr-1 negative, gated on the tip of the SP tail, were sorted into individual wells of 96-well plates. The mRNA of each cell was amplified by the global single cell RT-PCR method and biotinylated targets were generated after optimal digestion with DNAse I. Detection of the microarray hybridization signals was done according to the standard Affymetrix protocol (antibody amplified).
This sample is also known as HSL
Keywords = SP, side population, global single cell RT-PCR, GSC RT-PCR, microarray, HSC, stem cell


title: Single SP cell sample J (SCJ)

description:
Single SP cells from C57Bl/6 male mouse bone marrow, Sca-1 positive and Gr-1 negative, gated on the tip of the SP tail, were sorted into individual wells of 96-well plates. The mRNA of each cell was amplified by the global single cell RT-PCR method and biotinylated targets were generated after optimal digestion with DNAse I. Detection of the microarray hybridization signals was done according to the standard Affymetrix protocol (antibody amplified).
This sample is also known as HSJ
Keywords = SP, side population, global single cell RT-PCR, GSC RT-PCR, microarray, HSC, stem cell


title: Single SP cell sample K (SCK)

description:
Single SP cells from C57Bl/6 male mouse bone marrow, Sca-1 positive and Gr-1 negative, gated on the tip of the SP tail, were sorted into individual wells of 96-well plates. The mRNA of each cell was amplified by the global single cell RT-PCR method and biotinylated targets were generated after optimal digestion with DNAse I. Detection of the microarray hybridization signals was done according to the standard Affymetrix protocol (antibody amplified).
This sample is also known as HSK
Keywords = SP, side population, global single cell RT-PCR, GSC RT-PCR, microarray, HSC, stem cell


title: Single SP cell sample C (SCC)

description:
Single SP cells from C57Bl/6 male mouse bone marrow, Sca-1 positive and Gr-1 negative, gated on the tip of the SP tail, were sorted into individual wells of 96-well plates. The mRNA of each cell was amplified by the global single cell RT-PCR method and biotinylated targets were generated after optimal digestion with DNAse I. Detection of the microarray hybridization signals was done according to the standard Affymetrix protocol (antibody amplified).
This sample is also known as HSC
Keywords = SP, side population, global single cell RT-PCR, GSC RT-PCR, microarray, HSC, stem cell


title: ThreeRound_PCR

city: Ann Arbor

first: Scott

last: Tomlins

state: MI

address: 1400 E. Medical Center Dr., 5410 CCGC

department: Pathology

institute: University of Michigan

description: See MIAME checklist

organism: Homo sapiens

source_name: CPP, commercially available pooled benign prostate tissue total RNA, 25ng WTA three PCR, Cy5

source_name: CPP, commercially available pooled benign prostate tissue total RNA, 25ng WTA three PCR, Cy3


title: Single SP cell sample B (SCB)

description:
Single SP cells from C57Bl/6 male mouse bone marrow, Sca-1 positive and Gr-1 negative, gated on the tip of the SP tail, were sorted into individual wells of 96-well plates. The mRNA of each cell was amplified by the global single cell RT-PCR method and biotinylated targets were generated after optimal digestion with DNAse I. Detection of the microarray hybridization signals was done according to the standard Affymetrix protocol (antibody amplified).
This sample is also known as HSB
Keywords = SP, side population, global single cell RT-PCR, GSC RT-PCR, microarray, HSC, stem cell


title: Single SP cell sample D (SCD)

description:
Single SP cells from C57Bl/6 male mouse bone marrow, Sca-1 positive and Gr-1 negative, gated on the tip of the SP tail, were sorted into individual wells of 96-well plates. The mRNA of each cell was amplified by the global single cell RT-PCR method and biotinylated targets were generated after optimal digestion with DNAse I. Detection of the microarray hybridization signals was done according to the standard Affymetrix protocol (antibody amplified).
This sample is also known as HSD
Keywords = SP, side population, global single cell RT-PCR, GSC RT-PCR, microarray, HSC, stem cell


title: Single SP cell sample I (SCI)

description:
Single SP cells from C57Bl/6 male mouse bone marrow, Sca-1 positive and Gr-1 negative, gated on the tip of the SP tail, were sorted into individual wells of 96-well plates. The mRNA of each cell was amplified by the global single cell RT-PCR method and biotinylated targets were generated after optimal digestion with DNAse I. Detection of the microarray hybridization signals was done according to the standard Affymetrix protocol (antibody amplified).
This sample is also known as HSI
Keywords = SP, side population, global single cell RT-PCR, GSC RT-PCR, microarray, HSC, stem cell


title: Single SP cell sample F (SCF)

description:
Single SP cells from C57Bl/6 male mouse bone marrow, Sca-1 positive and Gr-1 negative, gated on the tip of the SP tail, were sorted into individual wells of 96-well plates. The mRNA of each cell was amplified by the global single cell RT-PCR method and biotinylated targets were generated after optimal digestion with DNAse I. Detection of the microarray hybridization signals was done according to the standard Affymetrix protocol (antibody amplified).
This sample is also known as HSF
Keywords = SP, side population, global single cell RT-PCR, GSC RT-PCR, microarray, HSC, stem cell


title: Single SP cell sample E (SCE)

description:
Single SP cells from C57Bl/6 male mouse bone marrow, Sca-1 positive and Gr-1 negative, gated on the tip of the SP tail, were sorted into individual wells of 96-well plates. The mRNA of each cell was amplified by the global single cell RT-PCR method and biotinylated targets were generated after optimal digestion with DNAse I. Detection of the microarray hybridization signals was done according to the standard Affymetrix protocol (antibody amplified).
This sample is also known as HSE
Keywords = SP, side population, global single cell RT-PCR, GSC RT-PCR, microarray, HSC, stem cell


title: Single SP cell sample G (SCG)

description:
Single SP cells from C57Bl/6 male mouse bone marrow, Sca-1 positive and Gr-1 negative, gated on the tip of the SP tail, were sorted into individual wells of 96-well plates. The mRNA of each cell was amplified by the global single cell RT-PCR method and biotinylated targets were generated after optimal digestion with DNAse I. Detection of the microarray hybridization signals was done according to the standard Affymetrix protocol (antibody amplified).
This sample is also known as HSG
Keywords = SP, side population, global single cell RT-PCR, GSC RT-PCR, microarray, HSC, stem cell


title: MultiRound_PCR

description: See MIAME checklist

source_name: CPP, commercially available pooled benign prostate tissue total RNA, 25ng WTA two PCR, Cy5

source_name: CPP, commercially available pooled benign prostate tissue total RNA, 25ng WTA four PCR, Cy3


title: Single SP cell equivalent sample A (SCEA)

description:
Forty bone marrow SP cells from C57Bl/6 male mouse bone marrow, Sca-1 positive and Gr-1 negative, gated on the tip of the SP tail, were sorted into 160 microliters of lysis buffer (40 times the amount used for single cells). 4-microliter aliquots (containing the mRNA equivalent to one single cell) were dispensed into individual wells of 96-well plates. The mRNA contained in each aliquot was amplified by the global single cell RT-PCR method and biotinylated targets were generated after optimal digestion with DNAse I. Detection of the microarray hybridization signals was done according to the standard Affymetrix protocol (antibody amplified).
This sample is also known as HWA
Keywords = SP, side population, global single cell RT-PCR, GSC RT-PCR, microarray, HSC, stem cell


title: Round2_PCR_from_Round1

description: See MIAME checklist

source_name: prostate tissue total RNA, 25ng WTA two PCR, Cy3


title: OneRound_PCR

description: See MIAME checklist

source_name: CPP, commercially available pooled benign prostate tissue total RNA, 25ng WTA one PCR


title: WTA_LCM_PCA4

description: See the MIAME checklist

source_name: LCM_PCA4, cancerous epithelium isolated by laser capture microdissection from a frozen prostate tissue sample, Cy5

source_name: CPP, Commercially available pool of benign prostate tissue total RNA, WTA two PCR, Cy3


title: TwoRound_PCR

description: See MIAME checklist

source_name: CPP, commercially available pooled benign prostate tissue total RNA, 25ng WTA two PCR, Cy3


title: WTA_LCM_NOR5

description: See the MIAME checklist

source_name: LCM_NOR5, Benign epithelium isolated by laser capture microdissection from a frozen prostate tissue sample, Cy5


title: WTA_LCM_STROMA1

description: See the MIAME checklist

source_name: LCM_STROMA1, Benign stroma adjacent to cancerous epithelium isolated by LCM from a frozen prostate tissue sample, Cy5


title: WTA_LCM_PCA1

description: See the MIAME checklist

source_name: LCM_PCA1, cancerous epithelium isolated by laser capture microdissection from a frozen prostate tissue sample, Cy5


title: FourRound_PCR

description: See MIAME checklist

source_name: CPP, commercially available pooled benign prostate tissue total RNA, 25ng WTA four PCR, Cy5


title: WTA_LCM_PCA2

description: See the MIAME checklist

source_name: LCM_PCA2, cancerous epithelium isolated by laser capture microdissection from a frozen prostate tissue sample, Cy5


title: WTA_LCM_NOR4

description: See the MIAME checklist

source_name: LCM_NOR4, Benign epithelium isolated by laser capture microdissection from a frozen prostate tissue sample, Cy5


title: Single SP cell sample A (SCA)

description:
Single SP cells from C57Bl/6 male mouse bone marrow, Sca-1 positive and Gr-1 negative, gated on the tip of the SP tail, were sorted into individual wells of 96-well plates. The mRNA of each cell was amplified by the global single cell RT-PCR method and biotinylated targets were generated after optimal digestion with DNAse I. Detection of the microarray hybridization signals was done according to the standard Affymetrix protocol (antibody amplified).
This sample is also known as HSA
Keywords = SP, side population, global single cell RT-PCR, GSC RT-PCR, microarray, HSC, stem cell

source_name: Bone marrow, tip of side population, Sca-1 positive, Gr-1 negative cell


title: WTA_LCM_STROMA2

description: See the MIAME checklist

source_name: LCM_STROMA2, Benign stroma adjacent to cancerous epithelium isolated by LCM from a frozen prostate tissue sample, Cy5


title: CD8 T lymphocytes sample D replicate 2 (10 cells)

description:
Ten cytotoxic T lymphocytes from C57Bl/6 male mouse bone marrow, CD8 and CD3 positive, were sorted into individual wells of 96-well plates. The mRNA of these cells was amplified by the global single cell RT-PCR method and biotinylated targets were generated after optimal digestion with DNAse I. Detection of the microarray hybridization signals was done according to the standard Affymetrix protocol (antibody amplified).
This sample is also known as XD2
Keywords = CD8, T cells, global single cell RT-PCR, GSC RT-PCR, microarray

source_name: Bone marrow, CD3 positive, CD8 positive cells


title: Upper SP sample O replicate 1 (10 cells)

description:
Ten SP cells from C57Bl/6 mouse bone marrow, Sca-1 positive and Gr-1 negative, gated on the UPPER third of the SP tail, were sorted into individual wells of 96-well plates. The mRNA of these cells was amplified by the global single cell RT-PCR method and biotinylated targets were generated after optimal digestion with DNAse I. Detection of the microarray hybridization signals was done according to the standard Affymetrix protocol (antibody amplified).
This sample is also known as UO1
Keywords = SP, side population, global single cell RT-PCR, GSC RT-PCR, microarray, HSC, stem cell

source_name: Bone marrow, upper portion of side population, Sca-1 positive, Gr-1 negative cells


title: CD8 T lymphocytes sample D replicate 1 (10 cells)

description:
Ten cytotoxic T lymphocytes from C57Bl/6 male mouse bone marrow, CD8 and CD3 positive, were sorted into individual wells of 96-well plates. The mRNA of these cells was amplified by the global single cell RT-PCR method and biotinylated targets were generated after optimal digestion with DNAse I. Detection of the microarray hybridization signals was done according to the standard Affymetrix protocol (antibody amplified).
This sample is also known as XD1
Keywords = CD8, T cells, global single cell RT-PCR, GSC RT-PCR, microarray


title: CD8 T lymphocytes sample C replicate 1 (10 cells)

description:
Ten cytotoxic T lymphocytes from C57Bl/6 male mouse bone marrow, CD8 and CD3 positive, were sorted into individual wells of 96-well plates. The mRNA of these cells was amplified by the global single cell RT-PCR method and biotinylated targets were generated after optimal digestion with DNAse I. Detection of the microarray hybridization signals was done according to the standard Affymetrix protocol (antibody amplified).
This sample is also known as XC1
Keywords = CD8, T cells, global single cell RT-PCR, GSC RT-PCR, microarray


title: Upper SP sample Z replicate 2 (10 cells)

description:
Ten SP cells from C57Bl/6 mouse bone marrow, Sca-1 positive and Gr-1 negative, gated on the UPPER third of the SP tail, were sorted into individual wells of 96-well plates. The mRNA of these cells was amplified by the global single cell RT-PCR method and biotinylated targets were generated after optimal digestion with DNAse I. Detection of the microarray hybridization signals was done according to the standard Affymetrix protocol (antibody amplified).
This sample is also known as UZ2
Keywords = SP, side population, global single cell RT-PCR, GSC RT-PCR, microarray, HSC, stem cell


title: CD8 T lymphocytes sample C replicate 2 (10 cells)

description:
Ten cytotoxic T lymphocytes from C57Bl/6 male mouse bone marrow, CD8 and CD3 positive, were sorted into individual wells of 96-well plates. The mRNA of these cells was amplified by the global single cell RT-PCR method and biotinylated targets were generated after optimal digestion with DNAse I. Detection of the microarray hybridization signals was done according to the standard Affymetrix protocol (antibody amplified).
This sample is also known as XC2
Keywords = CD8, T cells, global single cell RT-PCR, GSC RT-PCR, microarray


title: WTA_LCM_PCA3

description: See the MIAME checklist

source_name: LCM_PCA3, cancerous epithelium isolated by laser capture microdissection from a frozen prostate tissue sample, Cy5


title: CD8 T lymphocytes sample B replicate 1 (10 cells)

description:
Ten cytotoxic T lymphocytes from C57Bl/6 male mouse bone marrow, CD8 and CD3 positive, were sorted into individual wells of 96-well plates. The mRNA of these cells was amplified by the global single cell RT-PCR method and biotinylated targets were generated after optimal digestion with DNAse I. Detection of the microarray hybridization signals was done according to the standard Affymetrix protocol (antibody amplified).
This sample is also known as XB1
Keywords = CD8, T cells, global single cell RT-PCR, GSC RT-PCR, microarray


title: Upper SP sample N replicate 2 (10 cells)

description:
Ten SP cells from C57Bl/6 mouse bone marrow, Sca-1 positive and Gr-1 negative, gated on the UPPER third of the SP tail, were sorted into individual wells of 96-well plates. The mRNA of these cells was amplified by the global single cell RT-PCR method and biotinylated targets were generated after optimal digestion with DNAse I. Detection of the microarray hybridization signals was done according to the standard Affymetrix protocol (antibody amplified).
This sample is also known as UN2
Keywords = SP, side population, global single cell RT-PCR, GSC RT-PCR, microarray, HSC, stem cell


title: Upper SP sample O replicate 2 (10 cells)

description:
Ten SP cells from C57Bl/6 mouse bone marrow, Sca-1 positive and Gr-1 negative, gated on the UPPER third of the SP tail, were sorted into individual wells of 96-well plates. The mRNA of these cells was amplified by the global single cell RT-PCR method and biotinylated targets were generated after optimal digestion with DNAse I. Detection of the microarray hybridization signals was done according to the standard Affymetrix protocol (antibody amplified).
This sample is also known as UO2
Keywords = SP, side population, global single cell RT-PCR, GSC RT-PCR, microarray, HSC, stem cell


title: CD8 T lymphocytes sample B replicate 2 (10 cells)

description:
Ten cytotoxic T lymphocytes from C57Bl/6 male mouse bone marrow, CD8 and CD3 positive, were sorted into individual wells of 96-well plates. The mRNA of these cells was amplified by the global single cell RT-PCR method and biotinylated targets were generated after optimal digestion with DNAse I. Detection of the microarray hybridization signals was done according to the standard Affymetrix protocol (antibody amplified).
This sample is also known as XB2
Keywords = CD8, T cells, global single cell RT-PCR, GSC RT-PCR, microarray


title: Upper SP sample N replicate 1 (10 cells)

description:
Ten SP cells from C57Bl/6 mouse bone marrow, Sca-1 positive and Gr-1 negative, gated on the UPPER third of the SP tail, were sorted into individual wells of 96-well plates. The mRNA of these cells was amplified by the global single cell RT-PCR method and biotinylated targets were generated after optimal digestion with DNAse I. Detection of the microarray hybridization signals was done according to the standard Affymetrix protocol (antibody amplified).
This sample is also known as UN1
Keywords = SP, side population, global single cell RT-PCR, GSC RT-PCR, microarray, HSC, stem cell


title: Upper SP sample Z replicate 1 (10 cells)

description:
Ten SP cells from C57Bl/6 mouse bone marrow, Sca-1 positive and Gr-1 negative, gated on the UPPER third of the SP tail, were sorted into individual wells of 96-well plates. The mRNA of these cells was amplified by the global single cell RT-PCR method and biotinylated targets were generated after optimal digestion with DNAse I. Detection of the microarray hybridization signals was done according to the standard Affymetrix protocol (antibody amplified).
This sample is also known as UZ1
Keywords = SP, side population, global single cell RT-PCR, GSC RT-PCR, microarray, HSC, stem cell


title: WTA_LCM_NOR2

description: See the MIAME checklist

source_name: LCM_NOR2, Benign epithelium isolated by laser capture microdissection from a frozen prostate tissue sample, Cy5


title: WTA_LCM_NOR3

description: See the MIAME checklist

source_name: LCM_NOR3, Benign epithelium isolated by laser capture microdissection from a frozen prostate tissue sample, Cy5


title: Lower SP sample M replicate 2 (10 cells)

description:
Ten SP cells from C57Bl/6 male mouse bone marrow, Sca-1 positive and Gr-1 negative, gated on the LOWER third of the SP tail, were sorted into individual wells of 96-well plates. The mRNA of these cells was amplified by the global single cell RT-PCR method and biotinylated targets were generated after optimal digestion with DNAse I. Detection of the microarray hybridization signals was done according to the standard Affymetrix protocol (antibody amplified).
This sample is also known as LM2
Keywords = SP, side population, global single cell RT-PCR, GSC RT-PCR, microarray, HSC, stem cell

source_name: Bone marrow, lower portion of side population, Sca-1 positive, Gr-1 negative cells


title: WTA_LCM_NOR1

description: See the MIAME checklist

source_name: LCM_NOR1, Benign epithelium isolated by laser capture microdissection from a frozen prostate tissue sample, Cy5


title: WTA_FFPE_NOR1

description: See the MIAME checklist

source_name: Formalin-fixed paraffin embedded prostate tissue containing benign prostate, WTA two PCR, Cy5

source_name: CPP, Commercially available pool of benign prostate tissue total RNA, WTA two PCR, Cy3


title: Cell Line_WTA25ng_onePCR

description: See the MIAME checklist

source_name: LnCAP_R, prostate cancer cell line treated with 1nm R1881 for 24 hours, 25ng total RNA WTA one PCR, Cy5

source_name: LnCAP_C, prostate cancer cell line treated with ethanol control for 24 hours, 25ng total RNA WTA one PCR, Cy3


title: Cell Line_WTA25ng_twoPCR

description: See the MIAME checklist


title: Lower SP sample M replicate 1 (10 cells)

description:
Ten SP cells from C57Bl/6 male mouse bone marrow, Sca-1 positive and Gr-1 negative, gated on the LOWER third of the SP tail, were sorted into individual wells of 96-well plates. The mRNA of these cells was amplified by the global single cell RT-PCR method and biotinylated targets were generated after optimal digestion with DNAse I. Detection of the microarray hybridization signals was done according to the standard Affymetrix protocol (antibody amplified).
This sample is also known as LM1
Keywords = SP, side population, global single cell RT-PCR, GSC RT-PCR, microarray, HSC, stem cell


title: Cell Line_WTA10ng_twoPCR

description: See the MIAME checklist

source_name: LnCAP_R, prostate cancer cell line treated with 1nm R1881 for 24 hours, 10ng total RNA WTA two PCR, Cy5

source_name: LnCAP_C, prostate cancer cell line treated with ethanol control for 24 hours, 10ng total RNA WTA two PCR, Cy3


title: Cell Line_WTA100pg_twoPCR

description: See the MIAME checklist

source_name: LnCAP_R, prostate cancer cell line treated with 1nm R1881 for 24 hours, 100pg total RNA WTA two PCR, Cy5

source_name: LnCAP_C, prostate cancer cell line treated with ethanol control for 24 hours, 100pg total RNA WTA two PCR, Cy3


title: WTA_FFPE_PCA2

description: See the MIAME checklist

source_name: FFPE_PCA2, Formalin-fixed paraffin embedded prostate tissue containing localized adenocarcinoma, WTA two PCR, Cy5


title: Lower SP sample B replicate 1 (10 cells)

description:
Ten SP cells from C57Bl/6 male mouse bone marrow, Sca-1 positive and Gr-1 negative, gated on the LOWER third of the SP tail, were sorted into individual wells of 96-well plates. The mRNA of these cells was amplified by the global single cell RT-PCR method and biotinylated targets were generated after optimal digestion with DNAse I. Detection of the microarray hybridization signals was done according to the standard Affymetrix protocol (antibody amplified).
This sample is also known as LB1
Keywords = SP, side population, global single cell RT-PCR, GSC RT-PCR, microarray, HSC, stem cell


title: Lower SP sample A replicate 2 (10 cells)

description:
Ten SP cells from C57Bl/6 male mouse bone marrow, Sca-1 positive and Gr-1 negative, gated on the LOWER third of the SP tail, were sorted into individual wells of 96-well plates. The mRNA of these cells was amplified by the global single cell RT-PCR method and biotinylated targets were generated after optimal digestion with DNAse I. Detection of the microarray hybridization signals was done according to the standard Affymetrix protocol (antibody amplified).
This sample is also known as LA2
Keywords = SP, side population, global single cell RT-PCR, GSC RT-PCR, microarray, HSC, stem cell


title: Cell Line_WTA25ng_5mindeg_twoPCR

description:
See the MIAME checklist
five minutes degradation

source_name: LnCAP_R, prostate cancer cell line treated with 1nm R1881 for 24 hours, 25ng total RNA WTA two PCR, Cy5

source_name: LnCAP_C, prostate cancer cell line treated with ethanol control for 24 hours, 25ng total RNA WTA two PCR, Cy3


title: WTA_FFPE_PCA1

description: See the MIAME checklist

source_name: FFPE_PCA1, Formalin-fixed paraffin embedded prostate tissue containing localized adenocarcinoma, WTA two PCR, Cy5


title: Cell Line_WTA25ng_15mindeg_twoPCR

description:
See the MIAME checklist
15 minutes RNA degradation


title: Lower SP sample B replicate 2 (10 cells)

description:
Ten SP cells from C57Bl/6 male mouse bone marrow, Sca-1 positive and Gr-1 negative, gated on the LOWER third of the SP tail, were sorted into individual wells of 96-well plates. The mRNA of these cells was amplified by the global single cell RT-PCR method and biotinylated targets were generated after optimal digestion with DNAse I. Detection of the microarray hybridization signals was done according to the standard Affymetrix protocol (antibody amplified).
This sample is also known as LB2
Keywords = SP, side population, global single cell RT-PCR, GSC RT-PCR, microarray, HSC, stem cell


title: Cell Line_WTA10ng_onePCR

description: See the MIAME checklist

source_name: LnCAP_R, prostate cancer cell line treated with 1nm R1881 for 24 hours, 10ng total RNA WTA one PCR, Cy5

source_name: LnCAP_C, prostate cancer cell line treated with ethanol control for 24 hours, 10ng total RNA WTA one PCR, Cy3


title: Cell Line_Unamp

description: Please see the MIAME checklist

source_name: LnCAP_R, prostate cancer cell lines treated with 1nm synthetic androgen R1881 for 24 hours, 30ug unamplified total RNA, Cy5

source_name: LnCAP_C, prostate cancer cell lines treated with ethanol control for 24 hours, 30ug unamplified total RNA, Cy3


title: Cell Line_WTA1ng_onePCR

description: Please see the MIAME checklist

source_name: LnCAP_R, prostate cancer cell lines treated with 1nm synthetic androgen R1881 for 24 hours, 1ng RNA one WTA PCR, Cy5

source_name: LnCAP_C, prostate cancer cell lines treated with ethanol control for 24 hours, 1ng RNA one WTA PCR, Cy3


title: Tissue_WTA300ng3

description: See MIAME Checklist

source_name: PCA1, localized prostate adenocarcinoma tissue sample, 300ng RNA WTA one PCR, Cy5

source_name: CPP, Commercially available pool of benign prostate tissue total RNA, 300ng RNA WTA one PCR, Cy3


title: Low-Fat-Diet Rat

status: Public on Jan 01 2006

city: MORIYA City

first: Yutaka

last: OHTA

department: Fundamental Research Lab

country: Japan

institute: ASAHI Breweries

description:
High-Fat-Diet Experiment
We fed AIN93G Based food (Low-Fat).
4 weeks old SD-rat
9 weeks feedng

organism: Rattus norvegicus

source_name: Liver


title: High-Fat-Diet Rat

description:
High-Fat-Diet Experiment
We fed AIN93G Based food (High-Fat).
4 weeks old SD-rat
9 weeks feedng


title: Obese M 01 subA

city: Phoenix

first: Yong-Ho

last: Lee

state: AZ

department: Phoenix Epidemiology and Clinical Research Branch

institute: NIDDK, NIH

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies. Array type: HG_U95A. Subject: obese male Pima Indian (OM01).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression

source_name: adipocytes


title: Obese M 04 subA

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies. Array type: HG_U95A. Subject: obese male Pima Indian (OM04).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 05 subA

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies. Array type: HG_U95A. Subject: obese female Pima Indian (OF05).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Tissue_WTA300ng1

description: See MIAME Checklist


title: Lower SP sample A replicate 1 (10 cells)

description:
Ten SP cells from C57Bl/6 male mouse bone marrow, Sca-1 positive and Gr-1 negative, gated on the LOWER third of the SP tail, were sorted into individual wells of 96-well plates. The mRNA of these cells was amplified by the global single cell RT-PCR method and biotinylated targets were generated after optimal digestion with DNAse I. Detection of the microarray hybridization signals was done according to the standard Affymetrix protocol (antibody amplified).
This sample is also known as LA1
Keywords = SP, side population, global single cell RT-PCR, GSC RT-PCR, microarray, HSC, stem cell

source_name: Bone marrow, lower portion of side population, Sca-1 positive, Gr-1 negative cells


title: Obese M 03 subA

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies. Array type: HG_U95A. Subject: obese male Pima Indian (OM03).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 04 subA

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies. Array type: HG_U95A. Subject: lean male Pima Indian (LM04).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese M 02 subA

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies. Array type: HG_U95A. Subject: obese male Pima Indian (OM02).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Tissue_WTA75ng

description: See MIAME Checklist

source_name: PCA1, localized prostate adenocarcinoma tissue sample, 75ng RNA WTA one PCR, Cy5

source_name: CPP, Commercially available pool of benign prostate tissue total RNA, 75ng WTA one PCR, Cy3


title: Obese F 04 subA

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies. Array type: HG_U95A. Subject: obese female Pima Indian (OF04).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 02 subA

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies. Array type: HG_U95A. Subject: obese female Pima Indian (OF02).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Tissue_WTA300ng2

description: See MIAME Checklist


title: Obese F 03 subA

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies. Array type: HG_U95A. Subject: obese female Pima Indian (OF03).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 01 subA

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies. Array type: HG_U95A. Subject: obese female Pima Indian (OF01).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 03 subA

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies. Array type: HG_U95A. Subject: lean male Pima Indian (LM03).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Tissue_Unamp3

description: See MIAME Checklist

source_name: PCA1, localized prostate adenocarcinoma tissue sample, 30 ug total RNA, Cy5

source_name: CPP, Commercially available pool of benign prostate tissue total RNA, 30 ug total RNA, Cy3


title: Tissue_Unamp2

description: See MIAME Checklist


title: Tissue_WTA150ng

description: See MIAME Checklist

source_name: PCA1, localized prostate adenocarcinoma tissue sample, 150ng RNA WTA one PCR, Cy5

source_name: CPP, Commercially available pool of benign prostate tissue total RNA, 150ng RNA WTA one PCR, Cy3


title: Lean M 05 subA

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies. Array type: HG_U95A. Subject: lean male Pima Indian (LM05).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Tissue_WTA12_5ng

description: See MIAME Checklist

source_name: PCA1, localized prostate adenocarcinoma tissue sample, 12.5ng RNA WTA one PCR, Cy5

source_name: CPP, Commercially available pool of benign prostate tissue total RNA, 12.5ng RNA WTA one PCR, Cy3


title: Tissue_Unamp5

description: See MIAME Checklist


title: Tissue_Unamp4

description: See MIAME Checklist

source_name: CPP, Commercially available pool of benign prostate tissue total RNA, WTA two PCR, Cy3


title: Lean M 01 subA

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies. Array type: HG_U95A. Subject: lean male Pima Indian (LM01).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Tissue_WTA25ng

description: See MIAME Checklist

source_name: PCA1, localized prostate adenocarcinoma tissue sample, 25ng RNA WTA one PCR, Cy5

source_name: CPP, Commercially available pool of benign prostate tissue total RNA, 25ng RNA WTA one PCR, Cy3


title: Tissue_Unamp1

description: See MIAME Checklist


title: Lean F 04 subA

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies. Array type: HG_U95A. Subject: lean female Pima Indian (LF04).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 03 subA

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies. Array type: HG_U95A. Subject: lean female Pima Indian (LF03).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 02 subA

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies. Array type: HG_U95A. Subject: lean male Pima Indian (LM02).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 02 subA

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies. Array type: HG_U95A. Subject: lean female Pima Indian (LF02).

Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese M 08 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: obese male Pima Indian (OM08).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese M 07 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: obese male Pima Indian (OM07).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese M 09 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: obese male Pima Indian (OM09).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese M 06 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: obese male Pima Indian (OM06).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 05 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: obese female Pima Indian (OF05).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese M 03 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: obese male Pima Indian (OM03).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese M 04 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: obese male Pima Indian (OM04).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese M 02 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: obese male Pima Indian (OM02).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese M 05 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: obese male Pima Indian (OM05).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 01 subA

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.
Array type: HG_U95A.
Subject: lean female Pima Indian (LF01).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 05 subA

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies. Array type: HG_U95A. Subject: lean female Pima Indian (LF05).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese M 01 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: obese male Pima Indian (OM01).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 07 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: obese female Pima Indian (OF07).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 06 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: obese female Pima Indian (OF06).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 04 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: obese female Pima Indian (OF04).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 02 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: obese female Pima Indian (OF02).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 03 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: obese female Pima Indian (OF03).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 01 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: obese female Pima Indian (OF01).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 10 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: obese female Pima Indian (OF10).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 10 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: lean male Pima Indian (LM10).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 09 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: lean male Pima Indian (LM09).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 08 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: obese female Pima Indian (OF08).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 08 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: lean male Pima Indian (LM08).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 09 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: obese female Pima Indian (OF09).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 05 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: lean male Pima Indian (LM05).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 03 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: lean male Pima Indian (LM03).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 06 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: lean male Pima Indian (LM06).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 07 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: lean male Pima Indian (LM07).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 09 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: lean female Pima Indian (LF09).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 02 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: lean male Pima Indian (LM02).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 01 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: lean male Pima Indian (LM01).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 04 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: lean male Pima Indian (LM04).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 10 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: lean female Pima Indian (LF10).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 06 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: lean female Pima Indian (LF06).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 05 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: lean female Pima Indian (LF05).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 07 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: lean female Pima Indian (LF07).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 02 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: lean female Pima Indian (LF02).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 01 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies. Array type: HG_U95B. Subject: lean female Pima Indian (LF01).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese M 09 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: obese male Pima Indian (OM09).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 08 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: lean female Pima Indian (LF08).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese M 04 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: obese male Pima Indian (OM04).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese M 03 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: obese male Pima Indian (OM03).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese M 06 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: obese male Pima Indian (OM06).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 04 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: lean female Pima Indian (LF04).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese M 08 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: obese male Pima Indian (OM08).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese M 07 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: obese male Pima Indian (OM07).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 10 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: obese female Pima Indian (OF10).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese M 01 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: obese male Pima Indian (OM01).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese M 05 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: obese male Pima Indian (OM05).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 09 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: obese female Pima Indian (OF09).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 07 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: obese female Pima Indian (OF07).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese M 02 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: obese male Pima Indian (OM02).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 06 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: obese female Pima Indian (OF06).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 03 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: obese female Pima Indian (OF03).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 10 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: lean male Pima Indian (LM10).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 02 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: obese female Pima Indian (OF02).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 05 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: obese female Pima Indian (OF05).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 08 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: lean male Pima Indian (LM08).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 08 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: obese female Pima Indian (OF08).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 09 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: lean male Pima Indian (LM09).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 01 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: obese female Pima Indian (OF01).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 07 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: lean male Pima Indian (LM07).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 05 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: lean male Pima Indian (LM05).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 06 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: lean male Pima Indian (LM06).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 03 subB

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG_U95B. Subject: lean female Pima Indian (LF03).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 04 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: obese female Pima Indian (OF04).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 03 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: lean male Pima Indian (LM03).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 02 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: lean male Pima Indian (LM02).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 09 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: lean female Pima Indian (LF09).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 01 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: lean male Pima Indian (LM01).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 06 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: lean female Pima Indian (LF06).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 04 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: lean female Pima Indian (LF04).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 05 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: lean female Pima Indian (LF05).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese M 08 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: obese male Pima Indian (OM08).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 04 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: lean male Pima Indian (LM04).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 02 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: lean female Pima Indian (LF02).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 03 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: lean female Pima Indian (LF03).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 10 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: lean female Pima Indian (LF10).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 08 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: lean female Pima Indian (LF08).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese M 09 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: obese male Pima Indian (OM09).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 07 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: lean female Pima Indian (LF07).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese M 07 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: obese male Pima Indian (OM07).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 01 subD

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95D. Subject: lean female Pima Indian (LF01).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 08 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: obese female Pima Indian (OF08).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 07 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: obese female Pima Indian (OF07).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese M 02 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: obese male Pima Indian (OM02).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese M 03 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: obese male Pima Indian (OM03).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 09 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: obese female Pima Indian (OF09).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 05 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: obese female Pima Indian (OF05).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese M 06 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: obese male Pima Indian (OM06).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 04 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: obese female Pima Indian (OF04).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese M 01 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: obese male Pima Indian (OM01).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese M 04 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: obese male Pima Indian (OM04).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 06 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: lean male Pima Indian (LM06).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 09 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: lean male Pima Indian (LM09).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 02 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: obese female Pima Indian (OF02).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 10 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: lean male Pima Indian (LM10).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 05 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: lean male Pima Indian (LM05).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 01 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: lean male Pima Indian (LM01).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese M 05 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: obese male Pima Indian (OM05).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 01 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: obese female Pima Indian (OF01).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 03 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: lean male Pima Indian (LM03).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 02 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: lean male Pima Indian (LM02).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 06 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: obese female Pima Indian (OF06).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 10 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: obese female Pima Indian (OF10).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 04 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: lean male Pima Indian (LM04).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 03 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: obese female Pima Indian (OF03).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 09 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: lean female Pima Indian (LF09).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 08 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: lean male Pima Indian (LM08).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 10 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: lean female Pima Indian (LF10).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 02 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: lean female Pima Indian (LF02).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 08 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: lean female Pima Indian (LF08).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 07 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: lean male Pima Indian (LM07).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 01 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: lean female Pima Indian (LF01).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese M 07 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: obese male Pima Indian (OM07).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese M 06 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: obese male Pima Indian (OM06).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese M 08 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: obese male Pima Indian (OM08).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese M 05 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: obese male Pima Indian (OM05).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 07 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: lean female Pima Indian (LF07).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 06 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: lean female Pima Indian (LF06).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 03 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: lean female Pima Indian (LF03).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 05 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: lean female Pima Indian (LF05).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese M 02 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: obese male Pima Indian (OM02).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese M 09 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: obese male Pima Indian (OM09).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese M 01 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: obese male Pima Indian (OM01).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 10 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: obese female Pima Indian (OF10).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 09 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: obese female Pima Indian (OF09).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 08 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: obese female Pima Indian (OF08).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 05 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: obese female Pima Indian (OF05).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 06 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: obese female Pima Indian (OF06).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 07 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: obese female Pima Indian (OF07).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Pectin 9hrs, Rep1

city: Stillwater

first: Patricia

last: Ayoubi

state: OK

address: 246 Noble Research Center

department: Department of Biochemistry and Molecular Biology

institute: Oklahoma State University

description:
A. nidulans (RMS11) was pre-grown in liquid minimal medium containing 1% glucose.  After 18 h of growth, mycelia were collected, washed and transferred to fresh liquid minimal medium containing 1% Pectin or 1% glucose and grown at 37`C, pH 6.5.  Each culture was grown in quadruplicate for 9 hours and the tissue was harvested, blotted dry, frozen and lyophilized prior to RNA isolation.
RNA was isolated from lyophilized cultures using Trizol (Life Technologies, Rockville, MD) according to the manufacturers instructions. cDNA synthesis, labeling, and hybridization were performed using the Array350 3DNA detection system from Genisphere (Hatfield, PA) as recommended by the manufacturer.
Slides were scanning using a Scan Array Express from Perkin-Elmer and the intensity data were quantified using GenePix Pro 4.0 (Axon Inc.).  Pre-processing and normalization of data was accomplished using GenePix Autoprocessor (GPAP) (Ayoubi et al., unpublished results) to remove low intensity spots, remove poor quality spots, log2 transformation of background subtracted mean ratios and normalization using nonlinear print tip LOWESS normalization (Yang et al., 2002).  GPAP uses the R-project statistical environment (http://www.r-project.org) and Bioconductor (http://www.bioconductor.org) and is available at http://darwin.biochem.okstate.edu/gpap/.
Keywords = Aspergillus, Pectin, central metabolism, pectin, carbon catabolite repression, polysaccharide, exopolygalacturonase

organism: Aspergillus nidulans

source_name: Aspergillus nidulans grown on 1% Pectin, 9hrs

source_name: Aspergillus nidulans grown on 1% glucose, 9hrs


title: Obese F 04 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: obese female Pima Indian (OF04).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Pectin 9hrs, Rep2


title: Pectin 3hrs, Rep2

description:
A. nidulans (RMS11) was pre-grown in liquid minimal medium containing 1% glucose.  After 18 h of growth, mycelia were collected, washed and transferred to fresh liquid minimal medium containing 1% Pectin or 1% glucose and grown at 37`C, pH 6.5.  Each culture was grown in quadruplicate for 3 hours and the tissue was harvested, blotted dry, frozen and lyophilized prior to RNA isolation.
RNA was isolated from lyophilized cultures using Trizol (Life Technologies, Rockville, MD) according to the manufacturers instructions. cDNA synthesis, labeling, and hybridization were performed using the Array350 3DNA detection system from Genisphere (Hatfield, PA) as recommended by the manufacturer.
Slides were scanning using a Scan Array Express from Perkin-Elmer and the intensity data were quantified using GenePix Pro 4.0 (Axon Inc.).  Pre-processing and normalization of data was accomplished using GenePix Autoprocessor (GPAP) (Ayoubi et al., unpublished results) to remove low intensity spots, remove poor quality spots, log2 transformation of background subtracted mean ratios and normalization using nonlinear print tip LOWESS normalization (Yang et al., 2002).  GPAP uses the R-project statistical environment (http://www.r-project.org) and Bioconductor (http://www.bioconductor.org) and is available at http://darwin.biochem.okstate.edu/gpap/.
Keywords = Aspergillus, Pectin, central metabolism, pectin, carbon catabolite repression, polysaccharide, exopolygalacturonase

source_name: Aspergillus nidulans grown on 1% Pectin, 3hrs

source_name: Aspergillus nidulans grown on 1% glucose, 3hrs


title: Obese F 03 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: obese female Pima Indian (OF03).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression

source_name: adipocytes


title: Obese F 02 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: obese female Pima Indian (OF02).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Pectin 3hrs, Rep1


title: Lean M 09 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: lean male Pima Indian (LM09).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Starvation 9hrs, Rep2

description:
A. nidulans (RMS11) was pre-grown in liquid minimal medium containing 1% glucose.  After 18 h of growth, mycelia were collected, washed and transferred to fresh liquid minimal medium containing no carbon source or 1% glucose and grown at 37`C, pH 6.5.  Each culture was grown in quadruplicate for 9 hours and the tissue was harvested, blotted dry, frozen and lyophilized prior to RNA isolation.
RNA was isolated from lyophilized cultures using Trizol (Life Technologies, Rockville, MD) according to the manufacturers instructions. cDNA synthesis, labeling, and hybridization were performed using the Array350 3DNA detection system from Genisphere (Hatfield, PA) as recommended by the manufacturer.
Slides were scanning using a Scan Array Express from Perkin-Elmer and the intensity data were quantified using GenePix Pro 4.0 (Axon Inc.).  Pre-processing and normalization of data was accomplished using GenePix Autoprocessor (GPAP) (Ayoubi et al., unpublished results) to remove low intensity spots, remove poor quality spots, log2 transformation of background subtracted mean ratios and normalization using nonlinear print tip LOWESS normalization (Yang et al., 2002).  GPAP uses the R-project statistical environment (http://www.r-project.org) and Bioconductor (http://www.bioconductor.org) and is available at http://darwin.biochem.okstate.edu/gpap/.
Keywords = Aspergillus, starvation, central metabolism, pectin, carbon catabolite repression, polysaccharide, exopolygalacturonase

source_name: Aspergillus nidulans grown without carbon source, 9hrs


title: Lean M 07 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: lean male Pima Indian (LM07).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 04 subE

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95E. Subject: lean female Pima Indian (LF04).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 08 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: lean male Pima Indian (LM08).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese F 01 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: obese female Pima Indian (OF01).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Starvation 9hrs, Rep1


title: Lean M 05 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: lean male Pima Indian (LM05).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Starvation 3hrs, Rep2

description:
A. nidulans (RMS11) was pre-grown in liquid minimal medium containing 1% glucose.  After 18 h of growth, mycelia were collected, washed and transferred to fresh liquid minimal medium containing no carbon source or 1% glucose and grown at 37`C, pH 6.5.  Each culture was grown in quadruplicate for 3 hours and the tissue was harvested, blotted dry, frozen and lyophilized prior to RNA isolation.
RNA was isolated from lyophilized cultures using Trizol (Life Technologies, Rockville, MD) according to the manufacturers instructions. cDNA synthesis, labeling, and hybridization were performed using the Array350 3DNA detection system from Genisphere (Hatfield, PA) as recommended by the manufacturer.
Slides were scanning using a Scan Array Express from Perkin-Elmer and the intensity data were quantified using GenePix Pro 4.0 (Axon Inc.).  Pre-processing and normalization of data was accomplished using GenePix Autoprocessor (GPAP) (Ayoubi et al., unpublished results) to remove low intensity spots, remove poor quality spots, log2 transformation of background subtracted mean ratios and normalization using nonlinear print tip LOWESS normalization (Yang et al., 2002).  GPAP uses the R-project statistical environment (http://www.r-project.org) and Bioconductor (http://www.bioconductor.org) and is available at http://darwin.biochem.okstate.edu/gpap/.
Keywords = Aspergillus, starvation, central metabolism, pectin, carbon catabolite repression, polysaccharide, exopolygalacturonase

source_name: Aspergillus nidulans grown without carbon source, 3hrs


title: Lean M 04 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: lean male Pima Indian (LM04).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 06 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: lean male Pima Indian (LM06).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese M 04 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: obese male Pima Indian (OM04).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 02 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: lean male Pima Indian (LM02).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 03 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: lean male Pima Indian (LM03).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Starvation 3hrs, Rep1


title: Lean F 10 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: lean female Pima Indian (LF10).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Obese M 03 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: obese male Pima Indian (OM03).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 09 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: lean female Pima Indian (LF09).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 08 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: lean female Pima Indian (LF08).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 10 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: lean male Pima Indian (LM10).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 07 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: lean female Pima Indian (LF07).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean M 01 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: lean male Pima Indian (LM01).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 05 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: lean female Pima Indian (LF05).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 04 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: lean female Pima Indian (LF04).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 06 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: lean female Pima Indian (LF06).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 03 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: lean female Pima Indian (LF03).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 02 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: lean female Pima Indian (LF02).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Lean F 01 subC

description:
Adipocytes were isolated using collagenase digestion of abdominal subcutaneous fat needle biopsies.  Array type: HG-U95C. Subject: lean female Pima Indian (LF01).
Keywords = adipocyte
Keywords = obesity
Keywords = inflammation
Keywords = gene expression


title: Pectin 6hrs, Rep1

description:
A. nidulans (RMS11) was pre-grown in liquid minimal medium containing 1% glucose.  After 18 h of growth, mycelia were collected, washed and transferred to fresh liquid minimal medium containing 1% Pectin or 1% glucose and grown at 37`C, pH 6.5.  Each culture was grown in quadruplicate for 6 hours and the tissue was harvested, blotted dry, frozen and lyophilized prior to RNA isolation.
RNA was isolated from lyophilized cultures using Trizol (Life Technologies, Rockville, MD) according to the manufacturers instructions. cDNA synthesis, labeling, and hybridization were performed using the Array350 3DNA detection system from Genisphere (Hatfield, PA) as recommended by the manufacturer.
Slides were scanning using a Scan Array Express from Perkin-Elmer and the intensity data were quantified using GenePix Pro 4.0 (Axon Inc.).  Pre-processing and normalization of data was accomplished using GenePix Autoprocessor (GPAP) (Ayoubi et al., unpublished results) to remove low intensity spots, remove poor quality spots, log2 transformation of background subtracted mean ratios and normalization using nonlinear print tip LOWESS normalization (Yang et al., 2002).  GPAP uses the R-project statistical environment (http://www.r-project.org) and Bioconductor (http://www.bioconductor.org) and is available at http://darwin.biochem.okstate.edu/gpap/.
Keywords = Aspergillus, Pectin, central metabolism, pectin, carbon catabolite repression, polysaccharide, exopolygalacturonase

source_name: Aspergillus nidulans grown on 1% Pectin, 6hrs

source_name: Aspergillus nidulans grown on 1% glucose, 6hrs


title: Starvation 12hrs, Rep1

description:
A. nidulans (RMS11) was pre-grown in liquid minimal medium containing 1% glucose.  After 18 h of growth, mycelia were collected, washed and transferred to fresh liquid minimal medium containing no carbon source or 1% glucose and grown at 37`C, pH 6.5.  Each culture was grown in quadruplicate for 12 hours and the tissue was harvested, blotted dry, frozen and lyophilized prior to RNA isolation.
RNA was isolated from lyophilized cultures using Trizol (Life Technologies, Rockville, MD) according to the manufacturers instructions. cDNA synthesis, labeling, and hybridization were performed using the Array350 3DNA detection system from Genisphere (Hatfield, PA) as recommended by the manufacturer.
Slides were scanning using a Scan Array Express from Perkin-Elmer and the intensity data were quantified using GenePix Pro 4.0 (Axon Inc.).  Pre-processing and normalization of data was accomplished using GenePix Autoprocessor (GPAP) (Ayoubi et al., unpublished results) to remove low intensity spots, remove poor quality spots, log2 transformation of background subtracted mean ratios and normalization using nonlinear print tip LOWESS normalization (Yang et al., 2002).  GPAP uses the R-project statistical environment (http://www.r-project.org) and Bioconductor (http://www.bioconductor.org) and is available at http://darwin.biochem.okstate.edu/gpap/.
Keywords = Aspergillus, starvation, central metabolism, pectin, carbon catabolite repression, polysaccharide, exopolygalacturonase

source_name: Aspergillus nidulans grown without carbon source, 12hrs

source_name: Aspergillus nidulans grown on 1% glucose, 12hrs


title: Starvation 6hrs, Rep1

description:
A. nidulans (RMS11) was pre-grown in liquid minimal medium containing 1% glucose.  After 18 h of growth, mycelia were collected, washed and transferred to fresh liquid minimal medium containing no carbon source or 1% glucose and grown at 37`C, pH 6.5.  Each culture was grown in quadruplicate for 6 hours and the tissue was harvested, blotted dry, frozen and lyophilized prior to RNA isolation.
RNA was isolated from lyophilized cultures using Trizol (Life Technologies, Rockville, MD) according to the manufacturers instructions. cDNA synthesis, labeling, and hybridization were performed using the Array350 3DNA detection system from Genisphere (Hatfield, PA) as recommended by the manufacturer.
Slides were scanning using a Scan Array Express from Perkin-Elmer and the intensity data were quantified using GenePix Pro 4.0 (Axon Inc.).  Pre-processing and normalization of data was accomplished using GenePix Autoprocessor (GPAP) (Ayoubi et al., unpublished results) to remove low intensity spots, remove poor quality spots, log2 transformation of background subtracted mean ratios and normalization using nonlinear print tip LOWESS normalization (Yang et al., 2002).  GPAP uses the R-project statistical environment (http://www.r-project.org) and Bioconductor (http://www.bioconductor.org) and is available at http://darwin.biochem.okstate.edu/gpap/.
Keywords = Aspergillus, starvation, central metabolism, pectin, carbon catabolite repression, polysaccharide, exopolygalacturonase

source_name: Aspergillus nidulans grown without carbon source, 6hrs


title: Pectin 6hrs, Rep2


title: Starvation 12hrs, Rep2


title: Pectin 12hrs, Rep1

description:
A. nidulans (RMS11) was pre-grown in liquid minimal medium containing 1% glucose.  After 18 h of growth, mycelia were collected, washed and transferred to fresh liquid minimal medium containing 1% Pectin or 1% glucose and grown at 37`C, pH 6.5.  Each culture was grown in quadruplicate for 12 hours and the tissue was harvested, blotted dry, frozen and lyophilized prior to RNA isolation.
RNA was isolated from lyophilized cultures using Trizol (Life Technologies, Rockville, MD) according to the manufacturers instructions. cDNA synthesis, labeling, and hybridization were performed using the Array350 3DNA detection system from Genisphere (Hatfield, PA) as recommended by the manufacturer.
Slides were scanning using a Scan Array Express from Perkin-Elmer and the intensity data were quantified using GenePix Pro 4.0 (Axon Inc.).  Pre-processing and normalization of data was accomplished using GenePix Autoprocessor (GPAP) (Ayoubi et al., unpublished results) to remove low intensity spots, remove poor quality spots, log2 transformation of background subtracted mean ratios and normalization using nonlinear print tip LOWESS normalization (Yang et al., 2002).  GPAP uses the R-project statistical environment (http://www.r-project.org) and Bioconductor (http://www.bioconductor.org) and is available at http://darwin.biochem.okstate.edu/gpap/.
Keywords = Aspergillus, Pectin, central metabolism, pectin, carbon catabolite repression, polysaccharide, exopolygalacturonase

source_name: Aspergillus nidulans grown on 1% Pectin, 12hrs


title: Pectin 12hrs, Rep2


title: Starvation 6hrs, Rep2


title: Low Glucose Transcriptome Profiling {rpt 3}

city: Detroit

first: Lalit

middle: P

last: Singh

address: 4646 John R.

department: Internal Medicine/Nephrology & Physiology

institute: Wayne State University School of Medicine

description:
Identification of genes regulated by low glucose, high glucose and glucosamine treatments
Keywords = hexosamine
Keywords = glucose
Keywords = mesangial
Keywords = diabetes
Keywords = diabetic glomerulopathy

source_name: Mesangial Cells


title: Glucosamine Transcriptome Profiling {rpt}


title: High Glucose Transcriptome Profiling


title: Low Glucose Transcriptome Profiling {rpt}


title: Low Glucose Transcriptome Profiling


title: TGFBeta Transcriptome Profiling

description:
Identification of genes differentially regulated by TGFBeta1 in glomerular mesangial cells.
Keywords = hexosamine
Keywords = glucose
Keywords = mesangial
Keywords = diabetes
Keywords = diabetic glomerulopathy


title: TGFBeta Transcriptome Profiling {rpt}


title: High Glucose Transcriptome Profiling {rpt}