title: Embryo_20-21h_N=1_30048botGN

description:
Embryo samples were collected as one-hour egg lays which were allowed to develop for the desired interval. At least two pre-lays were performed before each collection. The embryos were dechorionated and snap frozen in liquid nitrogen. Aliquots from each collection were taken prior to freezing and fixed for morphological verification of developmental stage. Only tightly staged embryo collections were used for RNA isolation.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: 30048bot: Tmcrs; 20-21hr embryo  (bn)
Normalization Factor: Ratio of Medians=1.0325
Normalization Factor: Ratio of Means=1.02977
Normalization Factor: Median of Ratios=1.03524
Normalization Factor: Mean of Ratios=0.88237
Normalization Factor: Regression Ratio=1.13986
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Larvae_24h_N=2_700ii36bot

status: Public on Jan 06 2003

type: RNA

city: New Haven

first: Kevin

middle: P

last: White

state: CT

address: 333 Cedar Street

department: Department of Genetics

country: USA

institute: Yale University School of Medicine

description:
Larvae were raised from 6-hour embryonic egg lays, synchronized at hatching, and aged appropriately and stage-verified by morphology.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: Larvae_24h_N=2_700ii36bot
Normalization Factor: Ratio of Medians=1.05827
Normalization Factor: Ratio of Means=1.06243
Normalization Factor: Median of Ratios=1.05831
Normalization Factor: Mean of Ratios=0.941349
Normalization Factor: Regression Ratio=1.14072
Ratio Formulation:W1/W2 (635 nm/532 nm)

molecule: total RNA

organism: Drosophila melanogaster

source_name: reference


title: Larvae_105h_N=1_700ii3top

description:
Larvae were raised from 6-hour embryonic egg lays, synchronized at hatching, and aged appropriately and stage-verified by morphology.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: Larvae_105h_N=1_700ii3top
Normalization Factor: Ratio of Medians=1.05456
Normalization Factor: Ratio of Means=1.03782
Normalization Factor: Median of Ratios=1.06401
Normalization Factor: Mean of Ratios=0.786049
Normalization Factor: Regression Ratio=1.2202
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Larvae_57h_N=2_700ii39bot

description:
Larvae were raised from 6-hour embryonic egg lays, synchronized at hatching, and aged appropriately and stage-verified by morphology.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: Larvae_57h_N=2_700ii39bot
Normalization Factor: Ratio of Medians=1.28546
Normalization Factor: Ratio of Means=1.28358
Normalization Factor: Median of Ratios=1.28843
Normalization Factor: Mean of Ratios=1.09495
Normalization Factor: Regression Ratio=1.36964
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Larvae_33h_N=2_700ii36top

description:
Larvae were raised from 6-hour embryonic egg lays, synchronized at hatching, and aged appropriately and stage-verified by morphology.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: Larvae_33h_N=2_700ii36top
Normalization Factor: Ratio of Medians=1.13954
Normalization Factor: Ratio of Means=1.13768
Normalization Factor: Median of Ratios=1.14351
Normalization Factor: Mean of Ratios=0.950304
Normalization Factor: Regression Ratio=1.21935
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Larvae_105h_male_N=2_700ii4top

description:
Larvae were raised from 6-hour embryonic egg lays, synchronized at hatching, and aged appropriately and stage-verified by morphology.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: Larvae_105h_male_N=2_700ii4top
Normalization Factor: Ratio of Medians=0.994405
Normalization Factor: Ratio of Means=0.982996
Normalization Factor: Median of Ratios=0.998006
Normalization Factor: Mean of Ratios=0.739335
Normalization Factor: Regression Ratio=1.13552
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Larvae_49h_N=1_30092bot

description:
Larvae were raised from 6-hour embryonic egg lays, synchronized at hatching, and aged appropriately and stage-verified by morphology.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000 [46501]
Comment: tmcrs.: cy5=49hr larvae  (bn)
Normalization Factor: Ratio of Medians=1.45085
Normalization Factor: Ratio of Means=1.36922
Normalization Factor: Median of Ratios=1.42612
Normalization Factor: Mean of Ratios=1.18378
Normalization Factor: Regression Ratio=1.42759
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Larvae_24h_N=1_30037botGNrm

description:
Larvae were raised from 6-hour embryonic egg lays, synchronized at hatching, and aged appropriately and stage-verified by morphology.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: 30037bot:TmCrs; 24hr larvae  (bn)
Normalization Factor: Ratio of Medians=1.04407
Normalization Factor: Ratio of Means=1.01906
Normalization Factor: Median of Ratios=1.0321
Normalization Factor: Mean of Ratios=0.878088
Normalization Factor: Regression Ratio=1.10041
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Larvae_105h_female_N=2_700ii4bot

description:
Larvae were raised from 6-hour embryonic egg lays, synchronized at hatching, and aged appropriately and stage-verified by morphology.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: Larvae_105h_female_N=2_700ii4bot
Normalization Factor: Ratio of Medians=1.01239
Normalization Factor: Ratio of Means=1.00883
Normalization Factor: Median of Ratios=1.02187
Normalization Factor: Mean of Ratios=0.770116
Normalization Factor: Regression Ratio=1.18338
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Larvae_43h_N=1_700ii3bot

description:
Larvae were raised from 6-hour embryonic egg lays, synchronized at hatching, and aged appropriately and stage-verified by morphology.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: Larvae_43h_N=1_700ii3bot
Normalization Factor: Ratio of Medians=1.02559
Normalization Factor: Ratio of Means=1.02502
Normalization Factor: Median of Ratios=1.03158
Normalization Factor: Mean of Ratios=0.831545
Normalization Factor: Regression Ratio=1.13827
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Larvae_105h_N=2_700ii42top

description:
Larvae were raised from 6-hour embryonic egg lays, synchronized at hatching, and aged appropriately and stage-verified by morphology.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: Larvae_105h_N=2_700ii42top
Normalization Factor: Ratio of Medians=1.10475
Normalization Factor: Ratio of Means=1.1084
Normalization Factor: Median of Ratios=1.11238
Normalization Factor: Mean of Ratios=0.94188
Normalization Factor: Regression Ratio=1.18393
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Metamorph_72h_N=2_700ii97bot

description:
Pupal samples were synchronized by collection during the white prepupal stage, and aged appropriately.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: Metamorph_72h_N=2_700ii97bot
Normalization Factor: Ratio of Medians=1.0454
Normalization Factor: Ratio of Means=1.01932
Normalization Factor: Median of Ratios=1.04115
Normalization Factor: Mean of Ratios=0.92242
Normalization Factor: Regression Ratio=1.09416
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Embryo_13-14h_N=2_700ii27top

description:
Embryo samples were collected as one-hour egg lays which were allowed to develop for the desired interval. At least two pre-lays were performed before each collection. The embryos were dechorionated and snap frozen in liquid nitrogen. Aliquots from each collection were taken prior to freezing and fixed for morphological verification of developmental stage. Only tightly staged embryo collections were used for RNA isolation.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: Embryo_13-14h_N=2_700ii27top
Normalization Factor: Ratio of Medians=1.05437
Normalization Factor: Ratio of Means=1.07631
Normalization Factor: Median of Ratios=1.07531
Normalization Factor: Mean of Ratios=0.875012
Normalization Factor: Regression Ratio=1.21946
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Metamorph_96h_N=2_700ii55bot

description:
Pupal samples were synchronized by collection during the white prepupal stage, and aged appropriately.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: Metamorph_96h_N=2_700ii55bot
Normalization Factor: Ratio of Medians=0.99295
Normalization Factor: Ratio of Means=0.991388
Normalization Factor: Median of Ratios=0.991436
Normalization Factor: Mean of Ratios=0.88398
Normalization Factor: Regression Ratio=1.08157
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Metamorph_96h_N=1_700ii2top

description:
Pupal samples were synchronized by collection during the white prepupal stage, and aged appropriately.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: M 96.5h N=2 700ii2top
Normalization Factor: Ratio of Medians=1.02024
Normalization Factor: Ratio of Means=1.03483
Normalization Factor: Median of Ratios=1.06252
Normalization Factor: Mean of Ratios=0.789643
Normalization Factor: Regression Ratio=1.28854
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Metamorph_80h_N=2_700ii69top

description:
Pupal samples were synchronized by collection during the white prepupal stage, and aged appropriately.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: Metamorph_80h_N=2_700ii69top  11/13/00 collect'n, -no. males= no. females
Normalization Factor: Ratio of Medians=1.1699
Normalization Factor: Ratio of Means=1.08296
Normalization Factor: Median of Ratios=1.14522
Normalization Factor: Mean of Ratios=0.888532
Normalization Factor: Regression Ratio=1.18667
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Metamorph_04h_N=2_700ii7top

description:
Pupal samples were synchronized by collection during the white prepupal stage, and aged appropriately.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: Metamorph_04h_N=2_700ii7top
Normalization Factor: Ratio of Medians=1.07597
Normalization Factor: Ratio of Means=0.943778
Normalization Factor: Median of Ratios=1.07029
Normalization Factor: Mean of Ratios=0.832311
Normalization Factor: Regression Ratio=1.0137
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Larvae_105h_female_N=1_700ii45top

description:
Larvae were raised from 6-hour embryonic egg lays, synchronized at hatching, and aged appropriately and stage-verified by morphology.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: L 96hFemale 700ii45top
Normalization Factor: Ratio of Medians=1.22997
Normalization Factor: Ratio of Means=1.22518
Normalization Factor: Median of Ratios=1.23319
Normalization Factor: Mean of Ratios=1.03152
Normalization Factor: Regression Ratio=1.31204
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Larvae_105h_male_N=1_700ii45bot

description:
Larvae were raised from 6-hour embryonic egg lays, synchronized at hatching, and aged appropriately and stage-verified by morphology.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: L 96hMale 700ii45bot
Normalization Factor: Ratio of Medians=1.13177
Normalization Factor: Ratio of Means=1.1304
Normalization Factor: Median of Ratios=1.13484
Normalization Factor: Mean of Ratios=0.997504
Normalization Factor: Regression Ratio=1.21586
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Metamorph_72h_N=1_30098botGN

description:
Pupal samples were synchronized by collection during the white prepupal stage, and aged appropriately.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: 30098bot: Tmcrs; 72hr metamorphosis (bn)
Normalization Factor: Ratio of Medians=0.98582
Normalization Factor: Ratio of Means=0.992407
Normalization Factor: Median of Ratios=0.991012
Normalization Factor: Mean of Ratios=0.844696
Normalization Factor: Regression Ratio=1.1322
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Metamorph_60h_N=2_700ii69bot

description:
Pupal samples were synchronized by collection during the white prepupal stage, and aged appropriately.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: Metamorph_60h_N=2_700ii69bot  11/13/00 collect'n
Normalization Factor: Ratio of Medians=0.987859
Normalization Factor: Ratio of Means=0.93286
Normalization Factor: Median of Ratios=0.976215
Normalization Factor: Mean of Ratios=0.835766
Normalization Factor: Regression Ratio=0.982984
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Metamorph_36h_N=1_30068botGN

description:
Pupal samples were synchronized by collection during the white prepupal stage, and aged appropriately.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: 30068bot: Tmcrs; 36hr metamorphosis (bn)
Normalization Factor: Ratio of Medians=1.09416
Normalization Factor: Ratio of Means=1.1024
Normalization Factor: Median of Ratios=1.10174
Normalization Factor: Mean of Ratios=0.942878
Normalization Factor: Regression Ratio=1.23045
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Metamorph_42h_N=1_30068topGN

description:
Pupal samples were synchronized by collection during the white prepupal stage, and aged appropriately.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: 30068top: Tmcrs; 42hr metamorphosis (bn)
Normalization Factor: Ratio of Medians=1.09504
Normalization Factor: Ratio of Means=1.10648
Normalization Factor: Median of Ratios=1.10897
Normalization Factor: Mean of Ratios=0.910463
Normalization Factor: Regression Ratio=1.26008
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Metamorph_48h_N=1_30069botGN

description:
Pupal samples were synchronized by collection during the white prepupal stage, and aged appropriately.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: 30069bot: Tmcrs; 48hr metamorphosis (bn)
Normalization Factor: Ratio of Medians=1.01272
Normalization Factor: Ratio of Means=1.01578
Normalization Factor: Median of Ratios=1.01839
Normalization Factor: Mean of Ratios=0.879194
Normalization Factor: Regression Ratio=1.12313
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Metamorph_30h_N=1_30050topGN

description:
Pupal samples were synchronized by collection during the white prepupal stage, and aged appropriately.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: 30050top: Tmcrs; 30hr metamorphosis (bn)
Normalization Factor: Ratio of Medians=1.01407
Normalization Factor: Ratio of Means=1.0054
Normalization Factor: Median of Ratios=1.02058
Normalization Factor: Mean of Ratios=0.828979
Normalization Factor: Regression Ratio=1.13652
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Metamorph_48h_N=2_700ii32top

description:
Pupal samples were synchronized by collection during the white prepupal stage, and aged appropriately.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: Metamorph_48h_N=2_700ii32top
Normalization Factor: Ratio of Medians=1.05431
Normalization Factor: Ratio of Means=1.02773
Normalization Factor: Median of Ratios=1.06593
Normalization Factor: Mean of Ratios=0.888974
Normalization Factor: Regression Ratio=1.15303
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Metamorph_36h_N=2_700ii96bot

description:
Pupal samples were synchronized by collection during the white prepupal stage, and aged appropriately.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: Metamorph_36h_N=2_700ii96bot
Normalization Factor: Ratio of Medians=1.1183
Normalization Factor: Ratio of Means=1.13933
Normalization Factor: Median of Ratios=1.13458
Normalization Factor: Mean of Ratios=0.988261
Normalization Factor: Regression Ratio=1.22784
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Metamorph_24h_N=2_700ii32bot

description:
Pupal samples were synchronized by collection during the white prepupal stage, and aged appropriately.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: M 24h N=2 700ii32bot
Normalization Factor: Ratio of Medians=0.964824
Normalization Factor: Ratio of Means=0.89534
Normalization Factor: Median of Ratios=0.971043
Normalization Factor: Mean of Ratios=0.794187
Normalization Factor: Regression Ratio=0.989477
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Metamorph_20h_N=2_700ii23top

description:
Pupal samples were synchronized by collection during the white prepupal stage, and aged appropriately.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: M 20h N=2 700ii23top
Normalization Factor: Ratio of Medians=1.10702
Normalization Factor: Ratio of Means=1.08194
Normalization Factor: Median of Ratios=1.12625
Normalization Factor: Mean of Ratios=0.927734
Normalization Factor: Regression Ratio=1.19869
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Metamorph_24h_N=1_30066top2

description:
Pupal samples were synchronized by collection during the white prepupal stage, and aged appropriately.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: 30066top: Tmcrs; 24hr metamorphosis (bn)
Normalization Factor: Ratio of Medians=1.15487
Normalization Factor: Ratio of Means=1.09645
Normalization Factor: Median of Ratios=1.12889
Normalization Factor: Mean of Ratios=0.886276
Normalization Factor: Regression Ratio=1.24239
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Metamorph_16h_N=2_700ii100top

description:
Pupal samples were synchronized by collection during the white prepupal stage, and aged appropriately.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: Metamorph_16h_N=2_700ii100top  -used half of labelling rxn
Normalization Factor: Ratio of Medians=1.00481
Normalization Factor: Ratio of Means=1.0186
Normalization Factor: Median of Ratios=1.01989
Normalization Factor: Mean of Ratios=0.849236
Normalization Factor: Regression Ratio=1.15843
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Metamorph_12h_N=2_700ii8top

description:
Pupal samples were synchronized by collection during the white prepupal stage, and aged appropriately.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: Metamorph_12h_N=2_700ii8top
Normalization Factor: Ratio of Medians=1.15605
Normalization Factor: Ratio of Means=1.14158
Normalization Factor: Median of Ratios=1.17032
Normalization Factor: Mean of Ratios=0.808284
Normalization Factor: Regression Ratio=1.3622
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Metamorph_20h_N=1_30050botGN

description:
Pupal samples were synchronized by collection during the white prepupal stage, and aged appropriately.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: 30050bot: Tmcrs; 20hr metamorphosis (bn)
Normalization Factor: Ratio of Medians=0.97837
Normalization Factor: Ratio of Means=0.982761
Normalization Factor: Median of Ratios=0.980643
Normalization Factor: Mean of Ratios=0.84586
Normalization Factor: Regression Ratio=1.10904
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Metamorph_06h_N=1_30033bot2

description:
Pupal samples were synchronized by collection during the white prepupal stage, and aged appropriately.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: 30033bot: Tmcrs; 6hr metamorphosis (bn)
Normalization Factor: Ratio of Medians=0.997061
Normalization Factor: Ratio of Means=0.987339
Normalization Factor: Median of Ratios=0.990472
Normalization Factor: Mean of Ratios=0.842501
Normalization Factor: Regression Ratio=1.09203
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Metamorph_04h_N=1_30026topGN2

description:
Pupal samples were synchronized by collection during the white prepupal stage, and aged appropriately.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: 30026top: Tmcrs; 4hr metamorphosis (bn)
Normalization Factor: Ratio of Medians=0.988532
Normalization Factor: Ratio of Means=1.02465
Normalization Factor: Median of Ratios=1.00705
Normalization Factor: Mean of Ratios=0.764782
Normalization Factor: Regression Ratio=1.1948
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Metamorph_16h_N=1_30066bot

description:
Pupal samples were synchronized by collection during the white prepupal stage, and aged appropriately.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: 30066bot: Tmcrs; 16hr metamorphosis (bn)
Normalization Factor: Ratio of Medians=1.05963
Normalization Factor: Ratio of Means=1.00942
Normalization Factor: Median of Ratios=1.03544
Normalization Factor: Mean of Ratios=0.85034
Normalization Factor: Regression Ratio=1.1228
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Metamorph_10h_N=1_30090bot

description:
Pupal samples were synchronized by collection during the white prepupal stage, and aged appropriately.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: Metamorph_10h_N=1_30090bot  N=1
Normalization Factor: Ratio of Medians=1.10555
Normalization Factor: Ratio of Means=1.07623
Normalization Factor: Median of Ratios=1.11223
Normalization Factor: Mean of Ratios=0.880744
Normalization Factor: Regression Ratio=1.31549
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Embryo_05-6h_N=1_700ii2bot

description:
Embryo samples were collected as one-hour egg lays which were allowed to develop for the desired interval. At least two pre-lays were performed before each collection. The embryos were dechorionated and snap frozen in liquid nitrogen. Aliquots from each collection were taken prior to freezing and fixed for morphological verification of developmental stage. Only tightly staged embryo collections were used for RNA isolation.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: E 05-6h N=2 700ii2bot
Normalization Factor: Ratio of Medians=0.879903
Normalization Factor: Ratio of Means=0.916298
Normalization Factor: Median of Ratios=0.900151
Normalization Factor: Mean of Ratios=0.71908
Normalization Factor: Regression Ratio=1.05405
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Metamorph_08h_N=2_700ii11top

description:
Pupal samples were synchronized by collection during the white prepupal stage, and aged appropriately.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: M 08h N=2 700ii11top
Normalization Factor: Ratio of Medians=1.10769
Normalization Factor: Ratio of Means=0.978384
Normalization Factor: Median of Ratios=1.1009
Normalization Factor: Mean of Ratios=0.837481
Normalization Factor: Regression Ratio=1.07996
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Metamorph_30h_N=2_700ii26top

description:
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: Metamorph_30h_N=2_700ii26top
Normalization Factor: Ratio of Medians=0.931675
Normalization Factor: Ratio of Means=0.943281
Normalization Factor: Median of Ratios=0.952382
Normalization Factor: Mean of Ratios=0.758607
Normalization Factor: Regression Ratio=1.09494
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Metamorph_02h_N=2_700ii67top

description:
Pupal samples were synchronized by collection during the white prepupal stage, and aged appropriately.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: Metamorph_02h_N=2_700ii67top -9/15/99 collect'n
Normalization Factor: Ratio of Medians=1.07271
Normalization Factor: Ratio of Means=1.08264
Normalization Factor: Median of Ratios=1.08626
Normalization Factor: Mean of Ratios=0.89297
Normalization Factor: Regression Ratio=1.26058
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Metamorph_06h_N=2_700ii11bot

description:
Pupal samples were synchronized by collection during the white prepupal stage, and aged appropriately.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: M 06h N=2 700ii11bot
Normalization Factor: Ratio of Medians=1.07677
Normalization Factor: Ratio of Means=0.950915
Normalization Factor: Median of Ratios=1.06155
Normalization Factor: Mean of Ratios=0.85256
Normalization Factor: Regression Ratio=0.995778
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Metamorph_08h_N=1_30033topGN2

description:
Pupal samples were synchronized by collection during the white prepupal stage, and aged appropriately.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: 30033top: Tmcrs; 8hr metamorphosis (bn)
Normalization Factor: Ratio of Medians=1.08718
Normalization Factor: Ratio of Means=1.08593
Normalization Factor: Median of Ratios=1.07986
Normalization Factor: Mean of Ratios=0.882765
Normalization Factor: Regression Ratio=1.23534
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Adult_male_tudor_5d_N=2_700ii50bot(24)

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: A tud5d N=2 male700ii50botGNrm
Normalization Factor:Ratio of Medians - 1.03929
Normalization Factor:Ratio of Means - 1.04834
Normalization Factor:Median of Ratios - 1.02749
Normalization Factor:Mean of Ratios - 0.891453
Normalization Factor:Regression Ratio - 1.21065
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: Metamorph_12h_N=1_30032top

description:
Pupal samples were synchronized by collection during the white prepupal stage, and aged appropriately.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: 30032top: Tmcrs; 12hr metamorphosis (bn)
Normalization Factor: Ratio of Medians=1.13005
Normalization Factor: Ratio of Means=1.0639
Normalization Factor: Median of Ratios=1.09436
Normalization Factor: Mean of Ratios=0.825878
Normalization Factor: Regression Ratio=1.21269
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Metamorph_10h_N=2_700ii100bot

description:
Pupal samples were synchronized by collection during the white prepupal stage, and aged appropriately.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: Metamorph_10h_N=2_700ii100bot -used half of labelling rxn
Normalization Factor: Ratio of Medians=1.04509
Normalization Factor: Ratio of Means=1.05032
Normalization Factor: Median of Ratios=1.04974
Normalization Factor: Mean of Ratios=0.884558
Normalization Factor: Regression Ratio=1.15899
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Metamorph_60h_N=1_30069topGN

description:
Pupal samples were synchronized by collection during the white prepupal stage, and aged appropriately.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: 30069top: Tmcrs; 60hr metamorphosis (bn)
Normalization Factor: Ratio of Medians=1.02521
Normalization Factor: Ratio of Means=1.02929
Normalization Factor: Median of Ratios=1.02897
Normalization Factor: Mean of Ratios=0.86259
Normalization Factor: Regression Ratio=1.16393
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Metamorph_42h_N=2_700ii96top

description:
Pupal samples were synchronized by collection during the white prepupal stage, and aged appropriately.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: Metamorph_42h_N=2_700ii96top
Normalization Factor: Ratio of Medians=1.03443
Normalization Factor: Ratio of Means=1.0451
Normalization Factor: Median of Ratios=1.0803
Normalization Factor: Mean of Ratios=0.908183
Normalization Factor: Regression Ratio=1.15859
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Metamorph_00h_N=1_700ii67bot

description:
Pupal samples were synchronized by collection during the white prepupal stage, and aged appropriately.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: M 00h N=2 700ii67bot -no. males= no. females -half of a labelling rxn
Normalization Factor: Ratio of Medians=1.03113
Normalization Factor: Ratio of Means=1.04077
Normalization Factor: Median of Ratios=1.04494
Normalization Factor: Mean of Ratios=0.873937
Normalization Factor: Regression Ratio=1.18055
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Adult_male_25d_N=2_700ii94bot(25)

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: A 25d male700ii94bot
Normalization Factor:Ratio of Medians - 1.05778
Normalization Factor:Ratio of Means - 1.06276
Normalization Factor:Median of Ratios - 1.0592
Normalization Factor:Mean of Ratios - 0.888053
Normalization Factor:Regression Ratio - 1.18999
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: Adult_male_30d_N=2_700ii84top(25)

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: A 30d N=2 male700ii84top Grid Nrmlzd
Normalization Factor:Ratio of Medians - 1.07249
Normalization Factor:Ratio of Means - 1.03296
Normalization Factor:Median of Ratios - 1.03104
Normalization Factor:Mean of Ratios - 0.853713
Normalization Factor:Regression Ratio - 1.25696
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: Adult_male_tudor_5d_N=1_700ii12bot(25)2

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: A tud5d male700ii12bot
Normalization Factor:Ratio of Medians - 1.04072
Normalization Factor:Ratio of Means - 1.06145
Normalization Factor:Median of Ratios - 1.08139
Normalization Factor:Mean of Ratios - 0.847291
Normalization Factor:Regression Ratio - 1.22371
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: Adult_male_15d_N=2_700ii56bot(25)2

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: A 15d male700ii56bot
Normalization Factor:Ratio of Medians - 0.937292
Normalization Factor:Ratio of Means - 0.960153
Normalization Factor:Median of Ratios - 0.952478
Normalization Factor:Mean of Ratios - 0.802084
Normalization Factor:Regression Ratio - 1.0833
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: Adult_male_30d_N=1_700ii95bot(25)2

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: A 30d male700ii95bot
Normalization Factor:Ratio of Medians - 0.97778
Normalization Factor:Ratio of Means - 0.986516
Normalization Factor:Median of Ratios - 0.98401
Normalization Factor:Mean of Ratios - 0.833416
Normalization Factor:Regression Ratio - 1.0915
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: Adult_male_05d_N=1_30046top2

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: 30046top TmCrs: 5day male  (bn)
Normalization Factor:Ratio of Medians - 1.01071
Normalization Factor:Ratio of Means - 1.01932
Normalization Factor:Median of Ratios - 1.02185
Normalization Factor:Mean of Ratios - 0.860024
Normalization Factor:Regression Ratio - 1.15057
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: Adult_male_25d_N=1_700ii52top(25)2

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: A 25 d male700ii52top N=2
Normalization Factor:Ratio of Medians - 1.15361
Normalization Factor:Ratio of Means - 1.15446
Normalization Factor:Median of Ratios - 1.16234
Normalization Factor:Mean of Ratios - 0.941326
Normalization Factor:Regression Ratio - 1.27881
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: Adult_male_20d_N=1_700ii47top(24)2

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: A 20 d male700ii47top N=2
Normalization Factor:Ratio of Medians - 0.996204
Normalization Factor:Ratio of Means - 0.985606
Normalization Factor:Median of Ratios - 0.99131
Normalization Factor:Mean of Ratios - 0.764188
Normalization Factor:Regression Ratio - 1.1378
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: Adult_male_tudor_0-24h_N=1_700ii10bot(25)2

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: A tud0-24h male700ii10bot tudor line
Normalization Factor:Ratio of Medians - 1.09211
Normalization Factor:Ratio of Means - 1.12678
Normalization Factor:Median of Ratios - 1.13125
Normalization Factor:Mean of Ratios - 0.879405
Normalization Factor:Regression Ratio - 1.30864
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: Adult_male_10d_N=1_30040top2

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: 30040top TmCrs: 10day male  (bn)
Normalization Factor:Ratio of Medians - 0.963604
Normalization Factor:Ratio of Means - 0.971441
Normalization Factor:Median of Ratios - 0.972377
Normalization Factor:Mean of Ratios - 0.810861
Normalization Factor:Regression Ratio - 1.09799
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: Adult_male_10d_N=2_700ii46top(24)2

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: A 10 d male700ii46top  N=2
Normalization Factor:Ratio of Medians - 1.08317
Normalization Factor:Ratio of Means - 1.08862
Normalization Factor:Median of Ratios - 1.09158
Normalization Factor:Mean of Ratios - 0.887496
Normalization Factor:Regression Ratio - 1.2319
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: Adult_male_03d_N=2_700ii41top(24)2

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: A 03 d male700ii41top  N=2
Normalization Factor:Ratio of Medians - 1.30184
Normalization Factor:Ratio of Means - 1.28171
Normalization Factor:Median of Ratios - 1.28431
Normalization Factor:Mean of Ratios - 1.06683
Normalization Factor:Regression Ratio - 1.40755
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: Adult_male_tudor_0-24h_N=2_700ii50top(24)

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: A tud0-24h N=2 male700ii50top
Normalization Factor:Ratio of Medians - 1.1433
Normalization Factor:Ratio of Means - 1.13948
Normalization Factor:Median of Ratios - 1.1296
Normalization Factor:Mean of Ratios - 0.942401
Normalization Factor:Regression Ratio - 1.34256
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: Adult_female_tudor_5d_N=2_700ii68bot(25)2

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: A tud5d N=2 female700ii68botGNrm
Normalization Factor:Ratio of Medians - 1.11784
Normalization Factor:Ratio of Means - 1.0551
Normalization Factor:Median of Ratios - 1.07396
Normalization Factor:Mean of Ratios - 0.917113
Normalization Factor:Regression Ratio - 1.1655
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: Adult_male_0-24h_N=2_700ii53top(25)3

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: A 0-24d male700ii53top N=2
Normalization Factor:Ratio of Medians - 1.18786
Normalization Factor:Ratio of Means - 1.17804
Normalization Factor:Median of Ratios - 1.19739
Normalization Factor:Mean of Ratios - 0.968838
Normalization Factor:Regression Ratio - 1.30579
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: Adult_male_20d_N=2_700ii84bot(25)2

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: A 20d N=2 male700ii84bot
Normalization Factor:Ratio of Medians - 1.08921
Normalization Factor:Ratio of Means - 1.06526
Normalization Factor:Median of Ratios - 1.06358
Normalization Factor:Mean of Ratios - 0.912842
Normalization Factor:Regression Ratio - 1.22517
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: Adult_male_15d_N=1_30039top2

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: 30039topTmCrs: 15day male  (bn)
Normalization Factor:Ratio of Medians - 1.11033
Normalization Factor:Ratio of Means - 1.06777
Normalization Factor:Median of Ratios - 1.06252
Normalization Factor:Mean of Ratios - 0.802154
Normalization Factor:Regression Ratio - 1.23816
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: Adult_male_03d_N=1_male30028top

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: 3 day male  (bn)
Normalization Factor:Ratio of Medians - 1.03927
Normalization Factor:Ratio of Means - 1.01607
Normalization Factor:Median of Ratios - 1.05897
Normalization Factor:Mean of Ratios - 0.824399
Normalization Factor:Regression Ratio - 1.1768
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: Adult_female_tudor_0-24h_N=1_700ii10top(25)2

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: A_f_tud_0-24h_N=1_700ii10top(25)2
Normalization Factor:Ratio of Medians - 1.10339
Normalization Factor:Ratio of Means - 1.07367
Normalization Factor:Median of Ratios - 1.14589
Normalization Factor:Mean of Ratios - 0.76665
Normalization Factor:Regression Ratio - 1.36792
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: Adult_male_0-24h_N=1_30028bot2

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: 0-24hr male  (bn)
Normalization Factor:Ratio of Medians - 0.815555
Normalization Factor:Ratio of Means - 0.811964
Normalization Factor:Median of Ratios - 0.817833
Normalization Factor:Mean of Ratios - 0.702954
Normalization Factor:Regression Ratio - 0.899082
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: Adult_female_tudor_0-24h_N=2_700ii30top(25)2

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: A tud0-24h female700ii10top tudor line
Normalization Factor:Ratio of Medians - 1.04042
Normalization Factor:Ratio of Means - 1.08242
Normalization Factor:Median of Ratios - 1.06391
Normalization Factor:Mean of Ratios - 0.862907
Normalization Factor:Regression Ratio - 1.25749
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: Adult_female_tudor_5d_N=1_700ii12top(25)2

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: A tud5d female700ii12top
Normalization Factor:Ratio of Medians - 1.0556
Normalization Factor:Ratio of Means - 1.08575
Normalization Factor:Median of Ratios - 1.09368
Normalization Factor:Mean of Ratios - 0.862344
Normalization Factor:Regression Ratio - 1.24384
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: Adult_female_30d_N=2_700iI95top(25)2

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: A 30d female700ii95top
Normalization Factor:Ratio of Medians - 0.911308
Normalization Factor:Ratio of Means - 0.925506
Normalization Factor:Median of Ratios - 0.927589
Normalization Factor:Mean of Ratios - 0.747409
Normalization Factor:Regression Ratio - 1.0484
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: Adult_male_05d_N=2_700ii16bot(25)2

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: A 5d male700ii16bot
Normalization Factor:Ratio of Medians - 1.02251
Normalization Factor:Ratio of Means - 1.04754
Normalization Factor:Median of Ratios - 1.0341
Normalization Factor:Mean of Ratios - 0.874339
Normalization Factor:Regression Ratio - 1.18988
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: Adult_female_25d_N=2_700ii52bot(25)2

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: A 25 d female700ii52botN=2
Normalization Factor:Ratio of Medians - 0.834055
Normalization Factor:Ratio of Means - 0.843245
Normalization Factor:Median of Ratios - 0.844796
Normalization Factor:Mean of Ratios - 0.699632
Normalization Factor:Regression Ratio - 0.952006
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: Adult_female_30d_N=1_700ii30bot(25)2

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: A 30d female700ii30bot
Normalization Factor:Ratio of Medians - 0.992132
Normalization Factor:Ratio of Means - 1.02595
Normalization Factor:Median of Ratios - 1.00891
Normalization Factor:Mean of Ratios - 0.791988
Normalization Factor:Regression Ratio - 1.18717
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: Adult_female_05d_N=2_700ii16top(25)2

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: A 5d female700ii16top
Normalization Factor:Ratio of Medians - 0.9833
Normalization Factor:Ratio of Means - 1.05027
Normalization Factor:Median of Ratios - 1.05773
Normalization Factor:Mean of Ratios - 0.811927
Normalization Factor:Regression Ratio - 1.24146
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: Adult_female_10d_N=1_30040bot2

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: 30040botTmCrs: 10day female  (bn)
Normalization Factor:Ratio of Medians - 0.996764
Normalization Factor:Ratio of Means - 1.00707
Normalization Factor:Median of Ratios - 1.04908
Normalization Factor:Mean of Ratios - 0.857435
Normalization Factor:Regression Ratio - 1.12452
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: Adult_female_15d_N=2_700ii56top(25)2

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: A 15d female700ii56top
Normalization Factor:Ratio of Medians - 1.0345
Normalization Factor:Ratio of Means - 1.06196
Normalization Factor:Median of Ratios - 1.0507
Normalization Factor:Mean of Ratios - 0.849787
Normalization Factor:Regression Ratio - 1.22029
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: Adult_female_03d_N=2_700ii41bot(24)2

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: A 03 d female700ii41bot N=2
Normalization Factor:Ratio of Medians - 0.990112
Normalization Factor:Ratio of Means - 1.00357
Normalization Factor:Median of Ratios - 0.992689
Normalization Factor:Mean of Ratios - 0.871058
Normalization Factor:Regression Ratio - 1.12688
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: Adult_female_20d_N=2_700ii68top(25)2

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: A 20h N=2 female700ii68top
Normalization Factor:Ratio of Medians - 1.01623
Normalization Factor:Ratio of Means - 0.989699
Normalization Factor:Median of Ratios - 0.981062
Normalization Factor:Mean of Ratios - 0.801166
Normalization Factor:Regression Ratio - 1.19895
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: Adult_female_0-24h_N=2_700ii53bot(25)2

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: A 0-24d female700ii53bot   N=2
Normalization Factor:Ratio of Medians - 1.0806
Normalization Factor:Ratio of Means - 1.07971
Normalization Factor:Median of Ratios - 1.08603
Normalization Factor:Mean of Ratios - 0.916218
Normalization Factor:Regression Ratio - 1.18717
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: Adult_female_10d_N=2_700ii46bot(24)2

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: A 10 d female700ii46bot   N=2
Normalization Factor:Ratio of Medians - 1.0922
Normalization Factor:Ratio of Means - 1.09597
Normalization Factor:Median of Ratios - 1.11221
Normalization Factor:Mean of Ratios - 0.917672
Normalization Factor:Regression Ratio - 1.22738
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: Metamorph_02h_N=1_30026botGN

description:
Pupal samples were synchronized by collection during the white prepupal stage, and aged appropriately.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment:30026bot: Tmcrs; 2hr metamorphosis (bn)
NormalizationFactor:RatioOfMedians - 0.848731
NormalizationFactor:RatioOfMeans - 0.852341
NormalizationFactor:MedianOfRatios - 0.847411
NormalizationFactor:MeanOfRatios - 0.717464
NormalizationFactor:RegressionRatio - 0.948565
RatioFormulation - W1/W2 (635 nm/532 nm)
Creator:GenePix Pro 3.0.1.7


title: Metamorph_80h_N=1_30098topGN

description:
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: 30098top: Tmcrs; 80hr metamorphosis (bn)
Normalization Factor: Ratio of Medians=1.06606
Normalization Factor: Ratio of Means=1.07172
Normalization Factor: Median of Ratios=1.08009
Normalization Factor: Mean of Ratios=0.913459
Normalization Factor: Regression Ratio=1.19908
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: Adult_female_03d_N=1_30027top2

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: 3 day female  (bn)
Normalization Factor:Ratio of Medians - 0.647307
Normalization Factor:Ratio of Means - 0.660131
Normalization Factor:Median of Ratios - 0.657766
Normalization Factor:Mean of Ratios - 0.546236
Normalization Factor:Regression Ratio - 0.750829
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: Adult_female_25d_N=1_700iI94top(25)2

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: A 25d female700ii94top
Normalization Factor:Ratio of Medians - 1.0473
Normalization Factor:Ratio of Means - 1.06299
Normalization Factor:Median of Ratios - 1.05712
Normalization Factor:Mean of Ratios - 0.857684
Normalization Factor:Regression Ratio - 1.21374
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: Adult_female_0-24h_N=1_30027bot2

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: 0-24hr female  (bn)
Normalization Factor:Ratio of Medians - 0.905228
Normalization Factor:Ratio of Means - 0.919623
Normalization Factor:Median of Ratios - 0.912229
Normalization Factor:Mean of Ratios - 0.801387
Normalization Factor:Regression Ratio - 1.01604
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: Embryo_04.5-5.5h_N=1_30094top

description:
Embryo samples were collected as one-hour egg lays which were allowed to develop for the desired interval. At least two pre-lays were performed before each collection. The embryos were dechorionated and snap frozen in liquid nitrogen. Aliquots from each collection were taken prior to freezing and fixed for morphological verification of developmental stage. Only tightly staged embryo collections were used for RNA isolation.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000 [46501]
Comment:Timecourse: cy5=4.5-5.5hr embryo  (bn)
NormalizationFactor:RatioOfMedians - 0.837302
NormalizationFactor:RatioOfMeans - 0.843322
NormalizationFactor:MedianOfRatios - 0.838322
NormalizationFactor:MeanOfRatios - 0.748999
NormalizationFactor:RegressionRatio - 0.918602
RatioFormulation - W1/W2 (635 nm/532 nm)
Creator:GenePix Pro 3.0.1.7


title: Adult_female_05d_N=1_30046bot2

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: 30046bot TmCrs: 5day female  (bn)
Normalization Factor:Ratio of Medians - 1.02521
Normalization Factor:Ratio of Means - 1.02527
Normalization Factor:Median of Ratios - 1.03086
Normalization Factor:Mean of Ratios - 0.874822
Normalization Factor:Regression Ratio - 1.14592
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: FHCMV-H-2

status: Public on Nov 08 2002

city: Seattle

first: Lynn

last: Graf

state: WA

address: 1100 Fairview Ave. N. D1-100

department: Clinical Research Division

institute: Fred Hutchinson Cancer Research Center

description:
Target Source: human foreskin fibroblasts infected with cytomegalovirus
Description: Infection with clinical isolate FHCMV-H of CMV, genotype gB3. Overall comparison of gene expression profile of human foreskin fibroblasts (HFF) infected with 3 virus strains representing three glycoprotein B genotypes is described in (literature reference for manuscript).
Values are ratio of infected to uninfected fibroblasts.

HFF were plated in T75 flasks at approximately 80% confluence. The next day the supernatant was removed and replaced with the CMV viral inoculum (MOI of 3) in a total volume of 7 mls of RPMI supplemented with 5% FBS. The virus was permitted to absorb for 2 hours at 37°C and 5% CO2. The supernatants were removed from the non-infected control cells, replaced with 7 mls of RPMI, and incubated at the same time as the infected cultures. The inoculum was removed, the cells were washed 2 times with Hank's buffer, and media was replaced. Uninfected and infected fibroblasts were harvested at 24 hpi by brief trypsinization followed by pelleting of the cells. All RNA isolation was accomplished with Qiagen RNeasy Mini Kit  reagents. The RNA (25 ug) was annealed with 5 ug oligo dT12-18, and  reverse-transcribed into cDNA with Superscript II reverse transcriptase for 2h at 42°C in the presence of  0.5mM dGTP, 0.5mM dCTP, 0.5mM dATP, 0.3mM dTTP, 0.2mM amino-allyl dUTP. After hydrolysis of RNA in 0.2M NaOH, Tris was removed from the reaction with a Microcon-30 concentrator. The cDNA from uninfected and infected fibroblasts was covalently coupled separately with Cy5  and Cy3 monoreactive fluors, respectively, in 50mM sodium bicarbonate, pH 9.0, followed by quenching with 2.7M hydroxylamine. The Cy5 and Cy3 labelled cDNAs were combined and purified with a QIAquick PCR purification kit and suspended in 36 µl of 3X SSC and 0.8 mg/ml of poly-A for hybridization to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with a GenePix 4000A fluorescent scanner and image intensity data were extracted and analyzed with GenePix Pro 3.0 analysis software. After background correction and removal of flagged values, log base 2 expression ratios were mean centered and linear transformed to obtain the log and linear values given in the data table.

organism: Homo sapiens

source_name: CMV infected fibroblasts

source_name: uninfected fibroblasts


title: Larvae_33h_N=1_30064bot2

description:
Larvae were raised from 6-hour embryonic egg lays, synchronized at hatching, and aged appropriately and stage-verified by morphology.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment:30064bot: Tmcrs; 33hr larvae (bn)
NormalizationFactor:RatioOfMedians - 1.06483
NormalizationFactor:RatioOfMeans - 1.06129
NormalizationFactor:MedianOfRatios - 1.0659
NormalizationFactor:MeanOfRatios - 0.925377
NormalizationFactor:RegressionRatio - 1.1555
RatioFormulation - W1/W2 (635 nm/532 nm)
Creator:GenePix Pro 3.0.1.7


title: Adult_female_15d_30039bot

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment:30039botTmCrs: 15day female  (bn)
NormalizationFactor:RatioOfMedians - 1.01903
NormalizationFactor:RatioOfMeans - 1.01525
NormalizationFactor:MedianOfRatios - 1.05871
NormalizationFactor:MeanOfRatios - 0.857603
NormalizationFactor:RegressionRatio - 1.12964
RatioFormulation - W1/W2 (635 nm/532 nm)
Creator:GenePix Pro 3.0.1.7


title: E6:_U937_phoP _3h

status: Public on Jun 27 2002

city: Stanford

last: Stanford Microarray Database

state: CA

address: 300 Pasteur Drive

department: Stanford University, School of Medicine

institute: Stanford Microarray Database (SMD)

description:
Category: Pathogen
Subcategory: Salmonella
E5:_U937_ phoP _3h
Scanning Software: GenePix
Software version: Pro
Lot batch = shai072

source_name: U937/AGS ref, 2uL, 1st strand label

source_name: E6:_U937_phoP _3h, 1st strand label


title: Larvae_67h_N=1_30092top

description:
Larvae were raised from 6-hour embryonic egg lays, synchronized at hatching, and aged appropriately and stage-verified by morphology.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega). For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000 [46501]
Comment: tmcrs.: cy5=67hr larvae  (bn)
Normalization Factor: Ratio of Medians=1.26631
Normalization Factor: Ratio of Means=1.19389
Normalization Factor: Median of Ratios=1.24079
Normalization Factor: Mean of Ratios=1.06981
Normalization Factor: Regression Ratio=1.23199
Ratio Formulation:W1/W2 (635 nm/532 nm)


title: E6:_U937_ mock_2h

description:
Category: Pathogen
Subcategory: Salmonella
E6:_U937_ mock_2h
Scanning Software: GenePix
Software version: Pro
Lot batch = shai066

source_name: E6:_U937_ mock_2h, 1st strand label


title: E6:_U937_wild-type_2h

description:
Category: Pathogen
Subcategory: Salmonella
E6:_U937_wild-type_2h
Scanning Software: GenePix
Software version: Pro
Lot batch = shai064

source_name: E6:_U937_wild-type_2h, 1st strand label


title: E6:_U937_ phoP_2h

description:
Category: Pathogen
Subcategory: Salmonella
E6:_U937_ phoP_2h
Scanning Software: GenePix
Software version: Pro
Lot batch = shai065

source_name: E6:_U937_ phoP_2h, 1st strand label


title: E6:_U937_phoP_1h

description:
Category: Pathogen
Subcategory: Salmonella
E6:_U937_phoP_1h
Scanning Software: GenePix
Software version: Pro
Lot batch = shai069

source_name: E6:_U937_phoP_1h, 1st strand label


title: E6:_U937_ mock_4h

description:
Category: Pathogen
Subcategory: Salmonella
E6:_U937_ mock_4h
Scanning Software: GenePix
Software version: Pro
Lot batch = shai063

source_name: E6:_U937_ mock_4h, 1st strand label


title: E6:_U937_wild-type_2hb

description:
Category: Pathogen
Subcategory: Salmonella
Scanning Software: GenePix
Software version: Pro
Scanning parameters: =PMTVolts=940710LaserPower=12.98
Lot batch = shai079

source_name: AGS/U937 reference


title: E6:_U937_ wild-type_3h

description:
Category: Pathogen
Subcategory: Salmonella
E5:_U937_ wild-type_3h
Scanning Software: GenePix
Software version: Pro
Lot batch = shai071

source_name: E6:_U937_ wild-type_3h, 1st strand label


title: Adult_female_20d_N=1_700ii47bot(24)2

description:
Adult flies were synchronized by collection within the first 24 hours of eclosion (0-24 hour adults). The flies were cultured as a mixed population to the appropriate age, transferring to fresh food every 1-2 days. The adults were harvested by shaking into tubes and immediately snap freezing in liquid nitrogen. The animals were then sorted by sex, on a plastic sheet over dry ice.
Samples were prepared using the Canton S wild type strain and were raised and aged at 25°C. Total RNA was isolated using Trizol reagent (Gibco-BRL) and the PolyA+ was extracted using the PolyATtract mRNA isolation system (Promega).
For more details on Materials and Methods, see http://www.sciencemag.org/cgi/data/297/5590/2270/DC1/1
Scanner:GenePix 4000A
Comment: A 20 d female700ii47bot N=2
Normalization Factor:Ratio of Medians - 1.18139
Normalization Factor:Ratio of Means - 1.18207
Normalization Factor:Median of Ratios - 1.19139
Normalization Factor:Mean of Ratios - 0.990359
Normalization Factor:Regression Ratio - 1.32675
Ratio Formulation - W1/W2 (635 nm/532 nm)


title: E6:_U937_mock_3hr

description:
Category: Pathogen
Subcategory: Salmonella
E6:_U937_mock_3hr
Scanning Software: GenePix
Software version: Pro
Lot batch = shai070

source_name: E6:_U937_ mock_3hr, 1st strand label


title: E2:_U937_mock_3h

description:
Category: Pathogen
Subcategory: Salmonella
E2: U937mock 3h
Scanning Software: GenePix
Software version: 2.1.0.35
Lot batch = shv026

source_name: mock 3h


title: E2:_U937_wild-type_4hb

description:
Category: Pathogen
Subcategory: Salmonella
E2: U937 wild-type4hb
Scanning Software: GenePix
Software version: 2.1.0.35
Lot batch = shv025


title: E2:_U937_mock_2h

description:
Category: Pathogen
Subcategory: Salmonella
re-scanned 4/15/00 at higher PMTs; originally entered into data base as junkb
Scanning Software: GenePix
Software version: 2.1.0.35
Lot batch = shs055

source_name: U937/AGS ref, 2uL


title: E3:_U937_mock_2hb

description:
Category: Pathogen
Subcategory: Salmonella
mock 2h
Scanning Software: GenePix
Software version: 2.1.0.35
Lot batch = shv012

source_name: E3: U937 mock 2h


title: E2:_U937_wild-type_3h

description:
Category: Pathogen
Subcategory: Salmonella
E2: U937 wild-type 3h
Scanning Software: GenePix
Software version: 2.1.0.35
Lot batch = shv027

source_name: wild-type 3h


title: E3:_U937_mock_1hb

description:
Category: Pathogen
Subcategory: Salmonella
mock 1h
Scanning Software: GenePix
Software version: 2.1.0.35
Lot batch = shv009

source_name: E3: U937 mock 1h


title: E3:_U937_wild-type_2h

description:
Category: Pathogen
Subcategory: Salmonella
salm 2h
Scanning Software: GenePix
Software version: 2.1.0.35
Lot batch = shv013

source_name: E3: U937 salm 2h


title: E3:_U937_mock_0.5hb

description:
Category: Pathogen
Subcategory: Salmonella
E3:_U937_mock_0.5hb
Scanning Software: GenePix
Software version: Pro
Lot batch = shv030

source_name: U937/AGS ref, 2uL, 2nd strand label

source_name: E3:_U937_mock_0.5hb, 2nd strand label


title: E2:_U937_mock_2hb

description:
Category: Pathogen
Subcategory: Salmonella
Scanning Software: GenePix
Software version: 2.1.0.35
Lot batch = shv023

source_name: mock 2h


title: E3:_U937_phoP_0.5ha

description:
Category: Pathogen
Subcategory: Salmonella
phoP 30m
Scanning Software: GenePix
Software version: 2.1.0.35
Lot batch = shv007

source_name: E3: U937 phoP 30m


title: E2:_U937_wild-type_2h

description:
Category: Pathogen
Subcategory: Salmonella
E2:_U937_salm_2h
Scanning Software: GenePix
Software version: 2.1.0.35
Lot batch = shs065


title: E6:_U937_ wild-type_1h

description:
Category: Pathogen
Subcategory: Salmonella
E6:_U937_wild-type_1h
Scanning Software: GenePix
Software version: Pro
Lot batch = shai068

source_name: U937/AGS ref, 2uL, 1st strand label

source_name: E6:_U937_wild-type_1h, 1st strand label


title: E3:_U937_phoP_2hc

description:
Category: Pathogen
Subcategory: Salmonella
E3:_U937_phoP_2hc
Scanning Software: GenePix
Software version: Pro
Lot batch = shv031

source_name: E3:_U937_phoP_2hc, 2nd strand label


title: E3:_U937_wild-type_0.5h

description:
Category: Pathogen
Subcategory: Salmonella
E3: U937 salm 0.5h
Scanning Software: GenePix
Software version: 2.1.0.35
Lot batch = shs073

source_name: wild-type 0.5h


title: E3:_U937_wild-type_1hb

description:
Category: Pathogen
Subcategory: Salmonella
salm 1h
Scanning Software: GenePix
Software version: 2.1.0.35
Lot batch = shv010

source_name: E3: U937 salm 1h


title: E3:_U937_wild-type_4h

description:
Category: Pathogen
Subcategory: Salmonella
E3: U937 salm 4h
Scanning Software: GenePix
Software version: 2.1.0.35
Lot batch = shs075

source_name: salm 4h


title: E3:_U937_mock_4h

description:
Category: Pathogen
Subcategory: Salmonella
E3: U937 mock 4h
Scanning Software: GenePix
Software version: 2.1.0.35
Lot batch = shs074

source_name: mock 4h


title: E2:_U937_mock_1h

description:
Category: Pathogen
Subcategory: Salmonella
E2:_U937_mock_1h
Scanning Software: GenePix
Software version: 2.1.0.35
Lot batch = shs053


title: E2:_U937_mock_4hb

description:
Category: Pathogen
Subcategory: Salmonella
E2: U937 mock 4hb
Scanning Software: GenePix
Software version: 2.1.0.35
Lot batch = shv024

source_name: AGS/U937 reference


title: E2:_U937_wild-type_30m

description:
Category: Pathogen
Subcategory: Salmonella
E2:_U937_salm_30m
Scanning Software: GenePix
Software version: 2.1.0.35
Lot batch = shs052

source_name: U937/AGS ref, 2uL


title: E2:_U937_wild-type_1ha

description:
Category: Pathogen
Subcategory: Salmonella
E2:_U937_salm_1ha
Scanning Software: GenePix
Software version: 2.1.0.35
Lot batch = shs054


title: E2:_U937_mock_30m

description:
Category: Pathogen
Subcategory: Salmonella
E2:_U937_mock_30m
Scanning Software: GenePix
Software version: 2.1.0.35
Lot batch = shs051


title: E2:_U937_wild-type_1hb

description:
Category: Pathogen
Subcategory: Salmonella
E2:_U937_salm_1ha
Scanning Software: GenePix
Software version: 2.1.0.35
Lot batch = shs064