title: papilloma-7 - mAdbID:45846

description: mAdb experiment ID: 45846

source_name: ST 257B - control 2 - Cy5


title: carcinoma-6 - mAdbID:45716

status: Public on Feb 09 2006

type: RNA

city: Bethesda

first: Stuart Yuspa

last: Adam Glick

state: MD

address: 9000 Rockville Pike

department: National Cancer Institute

country: USA

institute: National Institutes of Health

description: mAdb experiment ID: 45716

hyb_protocol: cDNA Microarray Hybridization Other: 1 ¶l COT-1 DNA (8-10 ug/ul) and 1 ul poly A (8-10 ug/ul) were added to the labelled cDNA and then denatured at 100øC for 1 minute, followed by snap cooling on ice. (Final volume should be 20 ul.) Freshly made 2X Formamide hybridization buffer (50% formamide, 10X SSC, 0.2% SDS) was warmed to 42øC just before adding to samples. 20 ul of 2X F-hyb buffer to samples, and the total 40 ul of sample added to the microarray. 20 ul of 3X SSC was added to wells in hybridization chamber to maintain humidity. Array slides were incubated overnight (12-16 hours) at 42øC in water bath or hybridization oven. After hybridization of slides, slides were washed for 2 minute in 2X SSC with 0.1% SDS (with occasional plunging), for 2 minute in 1X SSC (with occasional plunging), for 2 minutes in 0.2X SSC (with occasional plunging), for 1 minute in 0.05X SSC, and spun for 3 minutes at 450 rpm to dry.

data_processing: After background correction and removal of flagged values, log base 2 expression ratios were median centered and linear transformed to obtain the log and linear values given in the data table.

molecule: total RNA

organism: Mus musculus

source_name: ST 241A- control 10-Cy5

label_protocol:
Cy5 Labeling
Label amount: 40000 pmol dye
Label method: Aminoallyl Coupling Method
Other: Protocol Similar to that found at the J. David Gladstone Institutes Genomic Core Laboratory (SOP #P004). Used CyeDye Post-Labelling Reactive Dye Pack.

extract_protocol:
RNA Extraction
Extraction method: Trizol-QIAgen RNeasy Kit
Other: Tumors were Homogenized in TRIzol, and RNA purified with the Quiagen RNeasy clean up kit.

treatment_protocol:
Treatment type: compound
Agent: Doxycycline
Treatment dose: 1 gram / kg chow
Treatment time: 48 hours
In-vivo treatment: Mice with tumors were treated with 1 gram/Kg doxycycline chow (Bio-Serve) after a 3-hour fast and tumors harvested after 48 hrs.
Treatment type: compound
Agent: DMBA/TPA
In-vivo treatment: Mice were shaved and initiated topically with 100ug of DMBA in 200ul of acetone. After 1 week mice were treated topically with 5 ug TPA in 200 ul acetone twice a week for 20 weeks. Benign tumors developed by 12-14 weeks of promotion, and SCC between 25-35 weeks after start of promotion. Tumor stage was verified by analysis of H&E stained sections.

tag: Strain

value: FVB

tag: Age

value: 7-12 Weeks

tag: Tissue

value: chemically-derived skin tumor

tag: Developmental stage

value: adult

tag: Disease state

value: Squamous Cell Carcinoma

tag: Genotype/Genetic variation

label_protocol:
Cy3 Labeling
Label amount: 40000 pmol dye
Label method: Aminoallyl Coupling Method
Other: Protocol Similar to that found at the J. David Gladstone Institutes Genomic Core Laboratory (SOP #P004). Used CyeDye Post-Labelling Reactive Dye Pack.

value: K5rTA tetOTGFbeta


title: papilloma-6 - mAdbID:47347

description: mAdb experiment ID: 47347

value: Papilloma


title: carcinoma-9 - mAdbID:45728

description: mAdb experiment ID: 45728

source_name: ST 257A- control 11-Cy3


title: papilloma-13 - mAdbID:37658

description: mAdb experiment ID: 37658

source_name: ST 2000J- control 6


title: carcinoma-8 - mAdbID:45724

description: mAdb experiment ID: 45724

source_name: ST 257A- control 11-Cy5


title: carcinoma-2 - mAdbID:46221

description: mAdb experiment ID: 46221

source_name: ST 242- control 9


title: carcinoma-7 - mAdbID:45731

description: mAdb experiment ID: 45731

source_name: ST 241A- control 10-Cy3


title: papilloma-10 - mAdbID:37262

description: mAdb experiment ID: 37262

source_name: ST 2000F- control 4


title: PC14

status: Public on Feb 10 2006

city: Tokyo

first: Akihiko

last: Gemma

address: Bumkyo-ku

country: Japan

institute: Nippon Medical School

description: adenocarcinoma cell line(Non-small)

hyb_protocol: standard Affymetrix procedures

scan_protocol: standard Affymetrix procedures

data_processing: Affymetrix Microarray Suite version 5.0

label: biotin

organism: Homo sapiens

source_name: human cancer cell line

label_protocol: Approximately 1µg of mRNA was processed to produce biotinylated cRNA targets.

extract_protocol: Total RNA was extracted from the cell lysate using TRIsol reagent (Invitrogen).mRNA was purified from total RNA with oligo-dT-magnetic beads (Toyobo)

tag: adenocarcinoma cell line(Non-small)


title: papilloma-14 - mAdbID:37591

description: mAdb experiment ID: 37591

source_name: ST 2000H- control 7


title: papilloma-12 - mAdbID:37588

description: mAdb experiment ID: 37588

source_name: ST 2000D- control 5


title: activated_normal_T_cells_static_rep3

city: Houston

first: Diana

last: Risin

state: TX

address: 2101 NASA Parkway

department: Space Medicine

institute: NASA/JSC

description: RNA quality check, labeling and hyb followed standard affymetrix procedures.

data_processing: RMA

label: biotinylation

tag: T-lymphocytes from normal donors were activated with anti-CD3 and IL2, then cultured under 1G condition for 24 hours


title: activated_normal_T_CELL_MMG

tag: T-lymphocytes from normal donors were activated with anti-CD3 and IL2 , then cultured in modeled MG (MMG) condition for 24 hours.


title: activated_normal_T_cells_MMG_rep3

tag: T-lymphocytes from normal donors were activated with anti-CD3 and IL2 , then cultured in MMG condition for 24 hours.


title: Activated_normal_T_cell_static

tag: T-lymphocytes from normal donors activated with anti-CD3 and IL2, cultured in 1G condition (normal gravity) for 24 hours.


title: Invitrogen yeast protoarray probed with YOR011W WW domain, second experiment

status: Public on Feb 08 2006

type: protein

city: Aurora

first: Jay

middle: R.

last: Hesselberth

state: CO

department: Biochemistry and Molecular Gentetics

institute: University of Colorado School of Medicine

description: WW domain GST-fusion protein expressed and purified from E. coli, and chemically biotinylated with Pierce NHS-LC-LC-biotin

hyb_protocol: Biotinylated fusion protein probes were applied at a concentration of 50 uM using conditions supplied by Invitrogen.  Interactions were visulalized by the application of Alexa Fluor 647-streptavavidin

scan_protocol: Fluorescent array images were collected for Cy5 with a GenePix 4000A fluorescent scanner and image intensity data were extracted and analyzed with GenePix Pro 3.0 analysis software.

data_processing: Each protein is printed in duplicate on the array; for each protein, the values of F635_MEAN - B635_MEAN were averaged (VALUE).  The arrays are printed in a total of 48 blocks.  The mean and standard deviation of signals from each block were calculated.  If a protein's VALUE was greater than three standard deviations above the mean for it's parent block, it was called significant.

label: Alexa Fluor 647

molecule: protein

organism: Saccharomyces cerevisiae

source_name: E.coli overexpression strain

extract_protocol: WW domain probes were purified as fusion proteins from E. coli overexpression strains

tag: biotinylated WW domain fusion protein


title: Invitrogen yeast protoarray probed with YKL012W WW domain 1, first experiment


title: activated_normal_T_cells_static_rep2

tag: T-lymphocytes from normal donors were activated with anti-CD3 and IL2 , then cultured in 1G (normal gravity) for 24 hours.


title: Invitrogen yeast protoarray probed with YKL012W WW domain 2, second experiment

description: WW domain MBP-fusion protein expressed and purified from E. coli, and chemically biotinylated with Pierce NHS-LC-LC-biotin


title: Invitrogen yeast protoarray probed with YKL012W WW domain 2, first experiment


title: Invitrogen yeast protoarray probed with YJL168C WW domain, second experiment


title: Invitrogen yeast protoarray probed with YJR017C WW domain, second experiment


title: Invitrogen yeast protoarray probed with YPR152C WW domain, first experiment


title: papilloma-9 - mAdbID:45848

description: mAdb experiment ID: 45848

source_name: ST 257CE - control 3 - Cy5

source_name: DT 228C


title: activated_Normal_T_cells_MMG_rep2

source_name: T-lymphocytes from normal donors were activated with anti-CD3 and IL2


title: Invitrogen yeast protoarray probed with YGL227W WW domain, first experiment


title: Invitrogen yeast protoarray probed with YIL030C WW domain, first experiment


title: Invitrogen yeast protoarray probed with YJL168C WW domain, first experiment


title: Invitrogen yeast protoarray probed with YJR017C WW domain, first experiment


title: Invitrogen yeast protoarray probed with YKL012W WW domain 1, second experiment


title: Invitrogen yeast protoarray probed with YOR011W WW domain, first experiment


title: Invitrogen yeast protoarray probed with YPR152C WW domain, second experiment


title: Invitrogen yeast protoarray probed with YIL030C WW domain, second experiment


title: papilloma-2 - mAdbID:50816

description: mAdb experiment ID: 50816


title: mouse VILI lps2 10K

status: Public on Feb 12 2006

city: Pittsburgh

first: Tamas

last: Dolinay

state: PA

department: Pulmonary, Allergy and Critical Care

institute: Univerisity of Pittsburgh Medical Center

description: mouse lps treatment2

data_processing: CodeLink Expression Analysis 2.3 (10K) and 4.1. (20K) sofware for initial analysis. Raw data was log2 base transformed and normalized with the Cyclic Loess method implemented in Bioconductor.  Cyclic Loess is described by Wu W, Dave N, Tseng G, Richards T, Xing EP, and Kaminski N.  in their publication entitled Comparison of normalization for Codelink bioarray data, published in BMC Bioinformatics 6: 309, 2005. The data submitted is median normalized.

source_name: BALB/c lung tissue

tag: BALB/c male, 22-30g


title: Invitrogen yeast protoarray probed with YFL010C WW domain, first experiment


title: Invitrogen yeast protoarray probed with YFL010C WW domain, second experiment


title: Invitrogen yeast protoarray probed with YER125W WW domain 2, first experiment


title: Invitrogen yeast protoarray probed with YER125W WW domain 2, second experiment


title: IL-2 KO + IL-2

city: Seattle

first: Jeffrey

last: Rasmussen

state: WA

department: Immunology

institute: University of Washington

description:
Sample isolated from CD4+GFPFoxp3+ T cells isolated from IL2-null mice after treatment with recombinant IL-2.

24 d old Il2-/- mice were injected intraperitoneally with 50 ng/g body weight of recombinant human IL-2 in PBS every 8 h for 24 h (total of four injections). After 24 h, lymph node and spleen suspensions were subjected to a combination of MACS and FACS sorting for RNA isolation as described in Nat Immunol. 2005 Nov;6(11):1142-51.

data_processing: GCRMA v 1.1.3, default settings

label: Biotin

source_name: CD4+ GFP-Foxp3+ from IL-2-null


title: Invitrogen yeast protoarray probed with YER125W WW domain 3, second experiment


title: Lymphoblasts_809-813_autistic vs nonautistic siblings

city: Washington

first: Valerie

middle: Wailin

last: Hu

state: DC

department: Biochemistry and Molecular Medicine

institute: The George Washington University School of Medicine and Health Sciences

description: Comparison of gene expression profiles of lymphoblastoid cell lines from autistic twin vs nonautistic sibling

data_processing: Log2 ratio of intensities from flip-dye experiment comparing expression profiles of autistic twin vs nonautistic sibling; Data analyses included normalization using local LOWESS followed by standard deviation regularization across individual subarrays, and flip-dye consistency checking for dye reversal replicates as implemented in TIGR MIDAS2.19 microarray analysis software.

source_name: Lymphoblastoid cell line HI0809, autistic twin

tag: Cell line

tag: Gender

value: male

value: lymphoblastoid cell line

source_name: Lymphoblastoid cell line HI0813, nonautistic sib


title: Lymphoblasts_2369-2368_autistic twins_rep2

description: Comparison of gene expression profiles of lymphoblastoid cell lines from monozygotic twins with differing severity of autism

data_processing: Log2 ratio of intensities from flip-dye experiment comparing expression profiles of monozygotic autistic twins; Data analyses included normalization using local LOWESS followed by standard deviation regularization across individual subarrays, and flip-dye consistency checking for dye reversal replicates as implemented in TIGR MIDAS2.19 microarray analysis software.

source_name: Lymphoblastoid cell line HI2369, autistic twin

source_name: Lymphoblastoid cell line HI2368, autistic twin


title: Invitrogen yeast protoarray probed with YER125W WW domain 1, second experiment


title: Invitrogen yeast protoarray probed with YER125W WW domain 3, first experiment


title: Lymphoblasts_2745-2744_nonautistic twins_rep2

description: Comparison of gene expression profiles of lymphoblastoid cell lines from nonautistic monozygotic twins

data_processing: Log2 ratio of intensities from flip-dye experiment comparing expression profiles of monozygotic nonautistic twins; Data analyses included normalization using local LOWESS followed by standard deviation regularization across individual subarrays, and flip-dye consistency checking for dye reversal replicates as implemented in TIGR MIDAS2.19 microarray analysis software.

source_name: Lymphoblastoid cell line HI2745, nonautistic twin

source_name: Lymphoblastoid cell line HI2744, nonautistic twin


title: Invitrogen yeast protoarray probed with YER125W WW domain 1, first experiment


title: Lymphoblasts_2369-2357_autistic vs nonautistic siblings_rep1

source_name: Lymphoblastoid cell line HI2369 autistic twin

source_name: Lymphoblastoid cell line HI2357, nonautistic sib


title: Lymphoblasts_2368-2357_milder autistic vs nonautistic siblings_rep2

description: Comparison of gene expression profiles of lymphoblastoid cell lines from milder autistic twin vs nonautistic sibling

data_processing: Log2 ratio of intensities from flip-dye experiment comparing expression profiles of milder autistic twin vs nonautistic sibling; Data analyses included normalization using local LOWESS followed by standard deviation regularization across individual subarrays, and flip-dye consistency checking for dye reversal replicates as implemented in TIGR MIDAS2.19 microarray analysis software.

source_name: Lymphoblastoid cell line HI2368, milder autistic twin


title: IL-2 KO no IL-2

description:
Sample isolated from CD4+GFPFoxp3+ T cells isolated from IL2-null mice after treatment with PBS (no IL-2)

24 d old Il2-/- mice were injected intraperitoneally with PBS every 8 h for 24 h (total of four injections). After 24 h, lymph node and spleen suspensions were subjected to a combination of MACS and FACS sorting for RNA isolation as described in Nat Immunol. 2005 Nov;6(11):1142-51.

source_name: CD4+ Foxp3-GFP+ from IL-2-null


title: Lymphoblasts_2368-2357_milder autistic vs nonautistic siblings_rep1


title: Invitrogen yeast protoarray probed with YGL227W WW domain, second experiment


title: Lymphoblasts_2595-2596_autistic twins_rep2

source_name: Lymphoblastoid cell line HI2595, autistic twin

source_name: Lymphoblastoid cell line HI2596, autistic twin


title: Lymphoblasts_2597-2598_autistic twins_rep1

source_name: Lymphoblastoid cell line HI2597, autistic twin

source_name: Lymphoblastoid cell line HI2598, autistic twin


title: Lymphoblasts_810-813_mildly autistic vs nonautistic siblings

description: Comparison of gene expression profiles of lymphoblastoid cell lines from mildly autistic twin vs nonautistic sibling

data_processing: Log2 ratio of intensities from flip-dye experiment comparing expression profiles of mildly autistic twin vs nonautistic sibling; Data analyses included normalization using local LOWESS followed by standard deviation regularization across individual subarrays, and flip-dye consistency checking for dye reversal replicates as implemented in TIGR MIDAS2.19 microarray analysis software.

source_name: Lymphoblastoid cell line HI0810, mildly autistic twin


title: Lymphoblasts_2597-2598_autistic twins_rep2


title: Lymphoblasts_2369-2357_autistic vs nonautistic siblings_rep2


title: REC (replicate 2)

city: Bellinzona

first: Francesco

last: Bertoni

state: Ticino

address: Via Vela 6

department: Experimental Oncology

country: Switzerland

institute: Institute of Southern Switzerland

description: human B cell lymphoma from peripherial blood

hyb_protocol: standard Affymetrix procedures using Fluidics Station 400

scan_protocol: standard Affymetrix procedures using GeneChip Scanner 3000

data_processing: Affymetrix GeneChip GCOS 1.1

source_name: REC

label_protocol: standard Affymetrix protocol starting from 10 µg of total RNA.

extract_protocol: RNA was extracted from cell lines with the Trizol method (Trizol, Invitrogen Life Technologies, San Diego, CA, USA) with an additional RNA clean-up step using the RNAeasy Purification kit.

tag: human B cell lymphoma established cell line


title: Lymphoblasts_809-810_autistic twins_rep2

source_name: Lymphoblastoid cell line HI0810, autistic twin


title: Lymphoblasts_2745-2744_nonautistic twins_rep1

source_name: Lymphoblastoid cell line HI1745, nonautistic twin


title: Lymphoblasts_361-360_autistic twins_rep2

source_name: Lymphoblastoid cell line HI0361, autistic twin

source_name: Lymphoblastoid cell line HI0360, autistic twin


title: Lymphoblasts_HI0361_autistic twin_rep3


title: Lymphoblasts_2595-2596_autistic twins_rep1


title: Lymphoblasts_809-810_autistic twins_rep1

source_name: Lymphoblastoid cell line HI0809, autistic twin


title: Lymphoblasts_361-360_autistic twins_rep1


title: Lymphoblasts_2369-2368_autistic twins_rep1

source_name: Lymphoblastoid cell line HI2369, autistic twin

source_name: Lymphoblastoid cell line HI2368, autistic twin

value: Male


title: JEKO-1 133A

tag: chuman B cell lymphoma established cell line


title: GRANTA-519 133A


title: NCEB-1 133A


title: 12h heat shock (2);HT-29;colon:carcinoma;heat shock_1hr;42999

city: San Diego

first: John

middle: R

last: Walker

state: CA

address: 10675 John Jay Hopkins

institute: Genomics Institute of the Novartis Research Foundation

description: 12h heat shock (2)

data_processing: gcRMA

label: 12h heat shock (2)


title: 12h H2O2 (1);HT-29;colon:carcinoma;Hydrogen peroxide;42996

description: 12h H2O2 (1)


title: 12h heat shock (1);HT-29;colon:carcinoma;heat shock_1hr;42998

description: 12h heat shock (1)

label: 12h heat shock (1)


title: REC (replicate 1)


title: 12h UV-50 (2);HT-29;colon:carcinoma;UV_50 J/m2;43001

description: 12h UV-50 (2)


title: 12h H2O2 (2);HT-29;colon:carcinoma;Hydrogen peroxide;42997

description: 12h H2O2 (2)


title: 12h UV-50 (1);HT-29;colon:carcinoma;UV_50 J/m2;43000

description: 12h UV-50 (1)


title: 12h UV-200 (2);HT-29;colon:carcinoma;UV_200 J/m2;43003

description: 12h UV-200 (2)


title: 12h control (2);HT-29;colon:carcinoma;NONE;42995

description: 12h control (2)

label: 12h control (2)


title: 12h control (1);HT-29;colon:carcinoma;NONE;42994

description: 12h control (1)

label: 12h control (1)


title: HC74 CB A

city: Cardiff

first: Lesley

last: Jones

address: Heath Park

department: Psychological Medicine

country: United Kingdom

institute: Cardiff University School of Medicine

description: human cerebellum

source_name: Cerebellum

label_protocol: Approximately 10 µg of total RNA was processed to produce biotinylated cRNA targets.

extract_protocol: ISOLATION OF TOTAL RNA USING TRIZOL, FOLLOWED BY RNeasy MINI KIT All equipment and solutions were RNAse-free: All solutions that are were made up in the lab and didn’t contain amines or flammables were treated with 0.01% diethylpyrocarbonate (DEPC, Sigma) with stirring overnight and then autoclaved to remove the DEPC (DEPC was diluted to a 10% v/v working stock in absolute ethanol first, as DEPC is immiscible with water). Wherever possible, solutions were purchased as RNAse-free or were molecular biology grade and reserved exclusively for RNA. Consummables direct from the supplier and reserved exclusively for RNA work were used. Equipment and benches were treated with proprietary solutions for decontaminating RNAses from surfaces (e.g. RNAseZAP from Ambion, following the manufacturer’s instructions). Materials Matrix Lysing D tubes (catalogue number 007190192) used for tissue homogenisation were purchased from VWR International Ltd, Poole, UK Trizol (catalogue number 15596-018) was from Invitrogen Ltd, Paisley, UK Isopropranol (catalogue number I-9516) was from Sigma-Aldrich Company Ltd, Gillingham RNeasy Mini Kit (catalogue number 74106) was from QIAGEN Ltd, Crawley, UK FastPrep machine for tissue homogenisation (used with Matrix Lysing D tubes) was purchased from Qbiogene-Alexis Ltd, Nottingham, UK). Method Trizol Extraction (essentially following supplied protocol): 1. 50-200mg appropriately dissected human brain was added to a Matrix Lysing D tube and either processed immediately or stored at -70°C until use. 1 ml of ice-cold TRIzol was added to the Matrix Lysing D tube. 2. The tubes were secured in the Fast Prep machine and homogenised on speed 4.0 for 30 seconds. 3. The TRIzol and sample were decanted into a 10 ml polypropylene tube. Another 1ml ice-cold TRIzol was added to the Lysing Matrix D tube and pipetted up and down a few times to ensure all of the sample was removed to the 10 ml polypropylene tube. The sample was incubated at room temperature for 5 minutes. l of chloroform was added to the sample and shaken vigourously?4. 400 for 15 seconds and then incubated at room temperature for 5 minutes. 5. The sample was spun at 12,000g for 15 minutes at 4ºC. 6. The upper aqueous phase was transferred to a fresh 10ml tube. 7. 1ml of isopropanol was added, mixed and left at room temperature for 10 minutes to precipitate. C.?8. The sample was spun at 12,000g for 10 minutes at 4 9. The supernatant was removed and the pellet washed carefully in 2 ml 75% ethanol. The tube was mixed gently to remove the pellet from the tube wall and ‘wash’ any residual isopropanol off the inside of the tube. C.?10. The sample was spun at 7,500g for 5 minutes at 4 11. The supernatant was removed and the pellet washed carefully in 2 ml 75% ethanol. The tube was mixed gently to remove the pellet from the tube wall and ‘wash’ any residual isopropanol off the inside of the tube. C.?12. The sample was spun at 7,500g for 5 minutes at 4 13. The supernatant was removed and the tube spun briefly to collect the residual ethanol at the bottom of the tube. All remaining ethanol was removed and the pellet was dried for 5 minutes in a 45ºC oven. l DEPC-treated water by?14. The pellet was resuspended in 100 C for 5 minutes and further vortexing.?vortexing, followed by 65 RNeasy Miniprep Extraction (essentially following supplied protocol): l ?–mercaptoethaol) was added to?l RLT buffer (containing 3.5?15. 350 the RNA sample and mixed thoroughly. l ethanol (96-100%) was then added and mixed thoroughly by?16. 250 pipetting. l) was immediately applied to an RNeasy mini column?17. The sample (700 placed in a 2ml collection tube and centrifuged for 1minute at 10,000g. The eluate was re-applied to the same RNeasy mini column and centrifuged for 1minute at 10,000g. The flow-through and collection tube were discarded. 18. The RNeasy column was transferred to a new 2ml collection tube and l Buffer RPE was applied to the RNeasy column. The column was?500 centrifuged for 1 minute at 10,000g. The eluate was discarded. l Buffer RPE was applied to the RNeasy column and?19. Another 500 centrifuged for 1 minute at 10,000g. The flow-through was discarded and the column was centrifuged for another minute at 10,000g. The column was turned 180º in the centrifuge and spun for another minute at 10,000g to dry the column. 20. To elute the RNA, the column was transferred to a new 1.5ml l* RNase-free water was applied directly on to the?collection tube. 30 RNeasy silica-gel membrane. After 2 minutes the column and tube were centrifuged for 1 minute at 10,000g. l* RNase-free water was applied directly onto the?21. A further 30 RNeasy silica-gel membrane. After 2 minutes the column and tube were centrifuged for 1 minute at 10,000g. The column was turned in the centrifuge 180º and spun for another minute at 10,000g. 22. The RNA concentration was determined using a spectrophotometer. The quality of the extraction was determined by the 260/280nm ratio which was around 1.8 for each sample. 23. An aliquot (<500ng) was analysed on an Agilent nano chip to check RNA integrity. RNA quality was assessed subjectively on a four point category: poor, o.k., good and excellent. This was used to select which samples to proceed to cDNA with. C prior to cDNA synthesis.?24. Total RNA was stored at -70 *The volume to elute the RNA into was determined empirically by the starting tissue weight and the size of the isopropanol pellet in the TRIzol part of the extraction in order to have the final concentration l so that further ethanol precipitation to?g/?in the range 1.1-2 concentrate the RNA was unnecessary in order to do first strand cDNA synthesis using 10µg total.

tag: HC74, human cerebellum HD grade 1, Genotype = 17/42, Age = 42, sex = F


title: HC66 CB A

description: human cerebellum

tag: HC66, human cerebellum HD grade 0, Genotype = 27/41, Age = 62, sex = M


title: HC72 CB A

description: human cerebellum

tag: HC72, human cerebellum HD grade 2, Genotype = 17/42, Age = 63, sex = F


title: HC80 CB A

description: human cerebellum

tag: HC80, human cerebellum HD grade 2, Genotype = 23/43, Age = 45, sex = F


title: HC82 CB A

description: human cerebellum

tag: HC82, human cerebellum HD grade 2, Genotype = 15/42, Age = 74, sex = M


title: HC86 CB A

description: human cerebellum

tag: HC86, human cerebellum HD grade 1, Genotype = 17/37, Age = 46, sex = M


title: HC69 CB A

description: human cerebellum

tag: HC69, human cerebellum HD grade 2, Genotype = 16/46, Age = 50, sex = F


title: HC81 CB A

description: human cerebellum

tag: HC81, human cerebellum HD grade 1, Genotype = 19/41, Age = 70, sex = F


title: HC73 CB A

description: human cerebellum

tag: HC73, human cerebellum HD grade 2, Genotype = 19/49, Age = 47, sex = M


title: HC68 CB A

description: human cerebellum

tag: HC68, human cerebellum HD grade 1, Genotype = 17/42, Age = 65, sex = F


title: HC62 CB A

description: human cerebellum

tag: HC62, human cerebellum HD grade 2, Genotype = 18/43, Age = 78, sex = F


title: HC65 CB A

description: human cerebellum

tag: HC65, human cerebellum HD grade 2, Genotype = 18/42, Age = 82, sex = F


title: HC71 CB A

description: human cerebellum

tag: HC71, human cerebellum HD grade 0, Genotype = 17/40, Age = 70, sex = F


title: NCEB-1 133B


title: JEKO-1 133B


title: GRANTA-519 133B


title: HC86 CB B

description: human cerebellum


title: HC55 CB A

description: human cerebellum

tag: HC55, human cerebellum HD grade 1, Genotype = 14/42, Age = 87, sex = M


title: HC57 CB A

description: human cerebellum

tag: HC57, human cerebellum HD grade 2, Genotype = 18/47, Age = 58, sex = M


title: HC53 CB A

description: human cerebellum

tag: HC53, human cerebellum HD grade 1, Genotype = 17/43, Age = 56, sex = M


title: HC105 CB A

description: human cerebellum

tag: HC105, human cerebellum HD grade 1, Genotype = 15/42, Age = 67, sex = F


title: HC81 CB B

description: human cerebellum


title: HC80 CB B

description: human cerebellum


title: HC74 CB B

description: human cerebellum

tag: HC74, human cerebellum HD grade 1, Genotype = 17/42, Age = 42, sex = F


title: HC82 CB B

description: human cerebellum


title: HC71 CB B

description: human cerebellum


title: HC72 CB B

description: human cerebellum


title: H131 CB A

description: human cerebellum

tag: H131, human cerebellum control, Genotype = 17/17, Age = 73, sex = F


title: H137 CB A

description: human cerebellum

tag: H137, human cerebellum control, Genotype = 18/20, Age = 77, sex = F


title: HC73 CB B

description: human cerebellum


title: HC69 CB B

description: human cerebellum


title: HC51 CB A

description: human cerebellum

tag: HC51, human cerebellum HD grade 1, Genotype = 16/43, Age = 58, sex = M


title: H132 CB A

description: human cerebellum

tag: H132, human cerebellum control, Genotype = 15/19, Age = 63, sex = F


title: H129 CB A

description: human cerebellum

tag: H129, human cerebellum control, Genotype = 20/21, Age = 48, sex = M


title: HC61 CB A

description: human cerebellum

tag: HC61, human cerebellum HD grade 2, Genotype = 17/46, Age = 65, sex = M


title: H126 CB A

description: human cerebellum

tag: H126, human cerebellum control, Genotype = 20/22, Age = 36, sex = F


title: HC66 CB B

description: human cerebellum

tag: HC66, human cerebellum HD grade 0, Genotype = 27/41, Age = 62, sex = M


title: HC103 CB A

description: human cerebellum

tag: HC103, human cerebellum HD grade 1, Genotype = 19/39, Age = 41, sex = M


title: H123 CB A

description: human cerebellum

tag: H123, human cerebellum control, Genotype = 17/20, Age = 78, sex = M


title: HC65 CB B

description: human cerebellum

tag: HC65, human cerebellum HD grade 2, Genotype = 18/42, Age = 82, sex = F


title: HC62 CB B

description: human cerebellum

tag: HC62, human cerebellum HD grade 2, Genotype = 18/43, Age = 78, sex = F


title: H124 CB A

description: human cerebellum

tag: H124, human cerebellum control, Genotype = 17/17, Age = 49, sex = M


title: HC68 CB B

description: human cerebellum

tag: HC68, human cerebellum HD grade 1, Genotype = 17/42, Age = 65, sex = F


title: HC61 CB B

description: human cerebellum


title: H121 CB A

description: human cerebellum

tag: H121, human cerebellum control, Genotype = 18/23, Age = 64, sex = F


title: HC55 CB B

description: human cerebellum

tag: HC55, human cerebellum HD grade 1, Genotype = 14/42, Age = 87, sex = M


title: HC103 CB B

description: human cerebellum


title: H115 CB A

description: human cerebellum

tag: H115, human cerebellum control, Genotype = 17/19, Age = 61, sex = M


title: H111 CB A

description: human cerebellum

tag: H111, human cerebellum control, Genotype = 17/20, Age = 46, sex = M


title: HC102 CB B

description: human cerebellum

tag: HC102, human cerebellum HD grade 3, Genotype = 17/42, Age = 65, sex = M


title: H104 CB A

description: human cerebellum

tag: H104, human cerebellum control, Genotype = 18/25, Age = 69, sex = M


title: H120 CB A

description: human cerebellum

tag: H120, human cerebellum control, Genotype = 18/22, Age = 62, sex = M


title: HC57 CB B

description: human cerebellum

tag: HC57, human cerebellum HD grade 2, Genotype = 18/47, Age = 58, sex = M


title: HC105 CB B

description: human cerebellum

tag: HC105, human cerebellum HD grade 1, Genotype = 15/42, Age = 67, sex = F


title: H85 CB B

description: human cerebellum

tag: H85, human cerebellum control, Genotype = 18/18, Age = 94, sex = M


title: H118 CB A

description: human cerebellum

tag: H118, human cerebellum control, Genotype = 15/16, Age = 57, sex = M


title: HC51 CB B

description: human cerebellum

tag: HC51, human cerebellum HD grade 1, Genotype = 16/43, Age = 58, sex = M


title: HC53 CB B

description: human cerebellum

tag: HC53, human cerebellum HD grade 1, Genotype = 17/43, Age = 56, sex = M


title: H110 CB A

description: human cerebellum

tag: H110, human cerebellum control, Genotype = 17/17, Age = 83, sex = F


title: H132 CB B

description: human cerebellum

tag: H132, human cerebellum control, Genotype = 15/19, Age = 63, sex = F


title: H137 CB B

description: human cerebellum

tag: H137, human cerebellum control, Genotype = 18/20, Age = 77, sex = F


title: H117 CB A

description: human cerebellum

tag: H117, human cerebellum control, Genotype = 17/17, Age = 64, sex = M


title: 76 CB A

description: human cerebellum

tag: 76, human cerebellum HD grade 4, Genotype = 15/48, Age = 42, sex = F


title: H129 CB B

description: human cerebellum

tag: H129, human cerebellum control, Genotype = 20/21, Age = 48, sex = M


title: 75 CB A

description: human cerebellum

tag: 75, human cerebellum HD grade 2, Genotype = 23/38, Age = 87, sex = M


title: H126 CB B

description: human cerebellum

tag: H126, human cerebellum control, Genotype = 20/22, Age = 36, sex = F


title: 79 CB A

description: human cerebellum

tag: 79, human cerebellum HD grade 2, Genotype = 17/44, Age = 57, sex = M


title: 81 CB A

description: human cerebellum

tag: 81, human cerebellum control, Genotype = 17/23, Age = 93, sex = F


title: H121 CB B

description: human cerebellum

tag: H121, human cerebellum control, Genotype = 18/23, Age = 64, sex = F


title: 82 CB A

description: human cerebellum

tag: 82, human cerebellum control, Genotype = 9/17, Age = 68, sex = M


title: H124 CB B

description: human cerebellum

tag: H124, human cerebellum control, Genotype = 17/17, Age = 49, sex = M


title: H131 CB B

description: human cerebellum

tag: H131, human cerebellum control, Genotype = 17/17, Age = 73, sex = F


title: 74 CB A

description: human cerebellum

tag: 74, human cerebellum HD grade 3, Genotype = 17/49, Age = 51, sex = M


title: 69 CB A

description: human cerebellum

tag: 69, human cerebellum HD grade 0, Genotype = 17/43, Age = 32, sex = F


title: 67 CB A

description: human cerebellum

tag: 67, human cerebellum control, Genotype = 13/17, Age = 68, sex = F


title: H115 CB B

description: human cerebellum

tag: H115, human cerebellum control, Genotype = 17/19, Age = 61, sex = M


title: 71 CB A

description: human cerebellum

tag: 71, human cerebellum HD grade 3, Genotype = 20/49, Age = 52, sex = M


title: 68 CB A

description: human cerebellum

tag: 68, human cerebellum HD grade 4, Genotype = 19/43, Age = 68, sex = M


title: 66 CB A

description: human cerebellum

tag: 66, human cerebellum control, Genotype = 17/11, Age = 51, sex = M


title: H111 CB B

description: human cerebellum

tag: H111, human cerebellum control, Genotype = 17/20, Age = 46, sex = M


title: H120 CB B

description: human cerebellum

tag: H120, human cerebellum control, Genotype = 18/22, Age = 62, sex = M


title: H117 CB B

description: human cerebellum

tag: H117, human cerebellum control, Genotype = 17/17, Age = 64, sex = M


title: H118 CB B

description: human cerebellum

tag: H118, human cerebellum control, Genotype = 15/16, Age = 57, sex = M


title: H110 CB B

description: human cerebellum

tag: H110, human cerebellum control, Genotype = 17/17, Age = 83, sex = F


title: H123 CB B

description: human cerebellum

tag: H123, human cerebellum control, Genotype = 17/20, Age = 78, sex = M


title: 70 CB A

description: human cerebellum

tag: 70, human cerebellum HD grade 3, Genotype = 21/45, Age = 56, sex = F


title: 39 CB A

description: human cerebellum

tag: 39, human cerebellum control, Genotype = 18/20, Age = 37, sex = F


title: 81 CB B

description: human cerebellum

tag: 81, human cerebellum control, Genotype = 17/23, Age = 93, sex = F


title: 82 CB B

description: human cerebellum

tag: 82, human cerebellum control, Genotype = 9/17, Age = 68, sex = M


title: H104 CB B

description: human cerebellum

tag: H104, human cerebellum control, Genotype = 18/25, Age = 69, sex = M


title: 38 CB A

description: human cerebellum

tag: 38, human cerebellum HD grade 4, Genotype = 20/48, Age = 37, sex = M


title: 40 CB A

description: human cerebellum

tag: 40, human cerebellum control, Genotype = 18/20, Age = 72, sex = M


title: 41 CB A

description: human cerebellum

tag: 41, human cerebellum control, Genotype = 18/23, Age = 67, sex = M


title: 79 CB B

description: human cerebellum

tag: 79, human cerebellum HD grade 2, Genotype = 17/44, Age = 57, sex = M


title: 75 CB B

description: human cerebellum

tag: 75, human cerebellum HD grade 2, Genotype = 23/38, Age = 87, sex = M


title: 74 CB B

description: human cerebellum

tag: 74, human cerebellum HD grade 3, Genotype = 17/49, Age = 51, sex = M


title: 32 CB A

description: human cerebellum

tag: 32, human cerebellum control, Genotype = 16/18, Age = 22, sex = M


title: 80 CB B

description: human cerebellum

tag: 80, human cerebellum control, Genotype = 18/19, Age = 61, sex = F


title: 34 CB A

description: human cerebellum

tag: 34, human cerebellum HD grade 1, Genotype = 16/45, Age = 34, sex = F


title: 76 CB B

description: human cerebellum

tag: 76, human cerebellum HD grade 4, Genotype = 15/48, Age = 42, sex = F


title: 33 CB A

description: human cerebellum

tag: 33, human cerebellum HD grade 4, Genotype = 17/44, Age = 59, sex = M


title: 72 CB B

description: human cerebellum

tag: 72, human cerebellum HD grade 2, Genotype = 17/45, Age = 42, sex = M


title: 31 CB A

description: human cerebellum

tag: 31, human cerebellum control, Genotype = 17/17, Age = 22, sex = M


title: 35 CB A

description: human cerebellum

tag: 35, human cerebellum HD grade 1, Genotype = 18/45, Age = 64, sex = M


title: 28 CB A

description: human cerebellum

tag: 28, human cerebellum HD grade 1, Genotype = 20/41, Age = 69, sex = F


title: HC102 CB A

description: human cerebellum

tag: HC102, human cerebellum HD grade 3, Genotype = 17/42, Age = 65, sex = M


title: 71 CB B

description: human cerebellum

tag: 71, human cerebellum HD grade 3, Genotype = 20/49, Age = 52, sex = M


title: 30 CB A

description: human cerebellum

tag: 30, human cerebellum control, Genotype = 17/19, Age = 37, sex = M


title: 70 CB B

description: human cerebellum

tag: 70, human cerebellum HD grade 3, Genotype = 21/45, Age = 56, sex = F


title: 67 CB B

description: human cerebellum

tag: 67, human cerebellum control, Genotype = 13/17, Age = 68, sex = F


title: 25 CB A

description: human cerebellum

tag: 25, human cerebellum HD grade 2, Genotype = 23/41, Age = 79, sex = F


title: 38 CB B

description: human cerebellum

tag: 38, human cerebellum HD grade 4, Genotype = 20/48, Age = 37, sex = M


title: 41 CB B

description: human cerebellum

tag: 41, human cerebellum control, Genotype = 18/23, Age = 67, sex = M


title: 40 CB B

description: human cerebellum

tag: 40, human cerebellum control, Genotype = 18/20, Age = 72, sex = M


title: 39 CB B

description: human cerebellum

tag: 39, human cerebellum control, Genotype = 18/20, Age = 37, sex = F


title: 27 CB A

description: human cerebellum

tag: 27, human cerebellum HD grade 1, Genotype = 19/46, Age = 19, sex = M


title: 66 CB B

description: human cerebellum

tag: 66, human cerebellum control, Genotype = 17/11, Age = 51, sex = M


title: HC86 FC BA4  A

description: human frontal cortex

source_name: Frontal Cortex

tag: HC86 FC human frontal cortex HD grade 1, Genotype = 17/37, Age = 46, sex = M


title: 23 CB A

description: human cerebellum

tag: 23, human cerebellum control, Genotype = 17/23, Age = 68, sex = F


title: 69 CB B

description: human cerebellum

tag: 69, human cerebellum HD grade 0, Genotype = 17/43, Age = 32, sex = F


title: 35 CB B

description: human cerebellum

tag: 35, human cerebellum HD grade 1, Genotype = 18/45, Age = 64, sex = M


title: 68 CB B

description: human cerebellum

tag: 68, human cerebellum HD grade 4, Genotype = 19/43, Age = 68, sex = M


title: 24 CB A

description: human cerebellum

tag: 24, human cerebellum HD grade 2, Genotype = 27/42, Age = 70, sex = M


title: 26 CB A

description: human cerebellum

tag: 26, human cerebellum HD grade 2, Genotype = 18/43, Age = 65, sex = M


title: HC81 FC BA4  A

description: human frontal cortex

tag: HC81 FC human frontal cortex HD grade 1, Genotype = 19/41, Age = 70, sex = F


title: HC72 FC BA4  A

description: human frontal cortex

tag: HC72 FC human frontal cortex HD grade 2, Genotype = 17/42, Age = 63, sex = F


title: 34 CB B

description: human cerebellum

tag: 34, human cerebellum HD grade 1, Genotype = 16/45, Age = 34, sex = F


title: HC74 FC BA4  A

description: human frontal cortex

tag: HC74 FC human frontal cortex HD grade 1, Genotype = 17/42, Age = 42, sex = F


title: 33 CB B

description: human cerebellum

tag: 33, human cerebellum HD grade 4, Genotype = 17/44, Age = 59, sex = M


title: HC80 FC BA4  A

description: human frontal cortex

tag: HC80 FC human frontal cortex HD grade 2, Genotype = 23/43, Age = 45, sex = F


title: HC69 FC BA4  A

description: human frontal cortex

tag: HC69 FC human frontal cortex HD grade 2, Genotype = 16/46, Age = 50, sex = F


title: 72 CB A

description: human cerebellum

tag: 72, human cerebellum HD grade 2, Genotype = 17/45, Age = 42, sex = M


title: HC68 FC BA4  A

description: human frontal cortex

tag: HC68 FC human frontal cortex HD grade 1, Genotype = 17/42, Age = 65, sex = F


title: 30 CB B

description: human cerebellum

tag: 30, human cerebellum control, Genotype = 17/19, Age = 37, sex = M


title: 31 CB B

description: human cerebellum

tag: 31, human cerebellum control, Genotype = 17/17, Age = 22, sex = M


title: 27 CB B

description: human cerebellum

tag: 27, human cerebellum HD grade 1, Genotype = 19/46, Age = 19, sex = M


title: 28 CB B

description: human cerebellum

tag: 28, human cerebellum HD grade 1, Genotype = 20/41, Age = 69, sex = F


title: 24 CB B

description: human cerebellum


title: 26 CB B

description: human cerebellum


title: 25 CB B

description: human cerebellum

tag: 25, human cerebellum HD grade 2, Genotype = 23/41, Age = 79, sex = F


title: 32 CB B

description: human cerebellum

tag: 32, human cerebellum control, Genotype = 16/18, Age = 22, sex = M


title: HC65 FC BA4  A

description: human frontal cortex

tag: HC65 FC human frontal cortex HD grade 2, Genotype = 18/42, Age = 82, sex = F


title: HC66 FC BA4  A

description: human frontal cortex

tag: HC66 FC human frontal cortex HD grade 0, Genotype = 27/41, Age = 62, sex = M


title: HC62 FC BA4  A

description: human frontal cortex

tag: HC62 FC human frontal cortex HD grade 2, Genotype = 18/43, Age = 78, sex = F


title: HC57 FC BA4  A

description: human frontal cortex

tag: HC57 FC human frontal cortex HD grade 2, Genotype = 18/47, Age = 58, sex = M


title: 23 CB B

description: human cerebellum

tag: 23, human cerebellum control, Genotype = 17/23, Age = 68, sex = F


title: HC55 FC BA4  A

description: human frontal cortex

tag: HC55 FC human frontal cortex HD grade 1, Genotype = 14/42, Age = 87, sex = M


title: HC53 FC BA4  A

description: human frontal cortex

tag: HC53 FC human frontal cortex HD grade 1, Genotype = 17/43, Age = 56, sex = M


title: HC86 FC BA4  B

description: human frontal cortex

tag: HC86 FC, human frontal cortex HD grade 1, Genotype = 17/37, Age = 46, sex = M


title: HC103 FC BA4  A

description: human frontal cortex

tag: HC103 FC human frontal cortex HD grade 1, Genotype = 19/39, Age = 41, sex = M


title: HC52 FC BA4  A

description: human frontal cortex

tag: HC52 FC human frontal cortex HD grade 2, Genotype = 19/46, Age = 61, sex = F


title: HC105 FC BA4  A

description: human frontal cortex

tag: HC105 FC human frontal cortex HD grade 1, Genotype = 15/42, Age = 67, sex = F


title: HC81 FC BA4  B

description: human frontal cortex

tag: HC81 FC, human frontal cortex HD grade 1, Genotype = 19/41, Age = 70, sex = F


title: HC61 FC BA4  A

description: human frontal cortex

tag: HC61 FC human frontal cortex HD grade 2, Genotype = 17/46, Age = 65, sex = M


title: HC102 FC BA4  A

description: human frontal cortex

tag: HC102 FC human frontal cortex HD grade 3, Genotype = 17/42, Age = 65, sex = M


title: H137 FC BA4  A

description: human frontal cortex

tag: H137 FC human frontal cortex control, Genotype = 18/20, Age = 77, sex = F


title: HC80 FC BA4  B

description: human frontal cortex

tag: HC80 FC, human frontal cortex HD grade 2, Genotype = 23/43, Age = 45, sex = F


title: HC74 FC BA4  B

description: human frontal cortex

tag: HC74 FC, human frontal cortex HD grade 1, Genotype = 17/42, Age = 42, sex = F


title: HC72 FC BA4  B

description: human frontal cortex

tag: HC72 FC, human frontal cortex HD grade 2, Genotype = 17/42, Age = 63, sex = F


title: 80 CB A

description: human cerebellum

tag: 80, human cerebellum control, Genotype = 18/19, Age = 61, sex = F


title: HC73 FC BA4  B

description: human frontal cortex

tag: HC73 FC, human frontal cortex HD grade 2, Genotype = 19/49, Age = 47, sex = M


title: H129 FC BA4  A

description: human frontal cortex

tag: H129 FC human frontal cortex control, Genotype = 20/21, Age = 48, sex = M


title: H126 FC BA4  A

description: human frontal cortex

tag: H126 FC human frontal cortex control, Genotype = 20/22, Age = 36, sex = F


title: HC69 FC BA4  B

description: human frontal cortex

tag: HC69 FC, human frontal cortex HD grade 2, Genotype = 16/46, Age = 50, sex = F


title: HC66 FC BA4  B

description: human frontal cortex

tag: HC66 FC, human frontal cortex HD grade 0, Genotype = 27/41, Age = 62, sex = M


title: H123 FC BA4  A

description: human frontal cortex

tag: H123 FC human frontal cortex control, Genotype = 17/20, Age = 78, sex = M


title: H128 FC BA4  A

description: human frontal cortex

tag: H128 FC human frontal cortex control, Genotype = 17/19, Age = 34, sex = F


title: H124 FC BA4  A

description: human frontal cortex

tag: H124 FC human frontal cortex control, Genotype = 17/17, Age = 49, sex = M


title: H121 FC BA4  A

description: human frontal cortex

tag: H121 FC human frontal cortex control, Genotype = 18/23, Age = 64, sex = F


title: HC62 FC BA4  B

description: human frontal cortex

tag: HC62 FC, human frontal cortex HD grade 2, Genotype = 18/43, Age = 78, sex = F


title: HC65 FC BA4  B

description: human frontal cortex

tag: HC65 FC, human frontal cortex HD grade 2, Genotype = 18/42, Age = 82, sex = F


title: H132 FC BA4  A

description: human frontal cortex

tag: H132 FC human frontal cortex control, Genotype = 15/19, Age = 63, sex = F


title: HC55 FC BA4  B

description: human frontal cortex

tag: HC55 FC, human frontal cortex HD grade 1, Genotype = 14/42, Age = 87, sex = M


title: HC61 FC BA4  B

description: human frontal cortex

tag: HC61 FC, human frontal cortex HD grade 2, Genotype = 17/46, Age = 65, sex = M


title: HC68 FC BA4  B

description: human frontal cortex

tag: HC68 FC, human frontal cortex HD grade 1, Genotype = 17/42, Age = 65, sex = F


title: H120 FC BA4  A

description: human frontal cortex

tag: H120 FC human frontal cortex control, Genotype = 18/22, Age = 62, sex = M


title: HC57 FC BA4  B

description: human frontal cortex

tag: HC57 FC, human frontal cortex HD grade 2, Genotype = 18/47, Age = 58, sex = M


title: HC53 FC BA4  B

description: human frontal cortex

tag: HC53 FC, human frontal cortex HD grade 1, Genotype = 17/43, Age = 56, sex = M


title: H117 FC BA4  A

description: human frontal cortex

tag: H117 FC human frontal cortex control, Genotype = 17/17, Age = 64, sex = M


title: H118 FC BA4  A

description: human frontal cortex

tag: H118 FC human frontal cortex control, Genotype = 15/16, Age = 57, sex = M


title: HC52 FC BA4  B

description: human frontal cortex

tag: HC52 FC, human frontal cortex HD grade 2, Genotype = 19/46, Age = 61, sex = F


title: H115 FC BA4  A

description: human frontal cortex

tag: H115 FC human frontal cortex control, Genotype = 17/19, Age = 61, sex = M


title: H113 FC BA4  A

description: human frontal cortex

tag: H113 FC human frontal cortex control, Genotype = 10/17, Age = 68, sex = M


title: HC105 FC BA4  B

description: human frontal cortex

tag: HC105 FC, human frontal cortex HD grade 1, Genotype = 15/42, Age = 67, sex = F


title: HC103 FC BA4  B

description: human frontal cortex

tag: HC103 FC, human frontal cortex HD grade 1, Genotype = 19/39, Age = 41, sex = M


title: H137 FC BA4  B

description: human frontal cortex

tag: H137 FC, human frontal cortex control, Genotype = 18/20, Age = 77, sex = F


title: H111 FC BA4  A

description: human frontal cortex

tag: H111 FC human frontal cortex control, Genotype = 17/20, Age = 46, sex = M


title: H104 FC BA4  A

description: human frontal cortex

tag: H104 FC human frontal cortex control, Genotype = 18/25, Age = 69, sex = M


title: H109 FC BA4  A

description: human frontal cortex

tag: H109 FC human frontal cortex control, Genotype = 15/18, Age = 81, sex = M


title: H132 FC BA4  B

description: human frontal cortex

tag: H132 FC, human frontal cortex control, Genotype = 15/19, Age = 63, sex = F


title: H128 FC BA4  B

description: human frontal cortex

tag: H128 FC, human frontal cortex control, Genotype = 17/19, Age = 34, sex = F


title: 99 FC BA9  A

description: human frontal cortex

tag: 99 FC human frontal cortex HD grade 4, Genotype = 15/48, Age = 42, sex = F


title: H131 FC BA4  B

description: human frontal cortex

tag: H131 FC, human frontal cortex control, Genotype = 17/17, Age = 73, sex = F


title: 96 FC BA9  A

description: human frontal cortex

tag: 96 FC human frontal cortex HD grade 3, Genotype = 17/49, Age = 51, sex = M


title: H126 FC BA4  B

description: human frontal cortex

tag: H126 FC, human frontal cortex control, Genotype = 20/22, Age = 36, sex = F


title: H124 FC BA4  B

description: human frontal cortex

tag: H124 FC, human frontal cortex control, Genotype = 17/17, Age = 49, sex = M


title: 97 FC BA9  A

description: human frontal cortex

tag: 97 FC human frontal cortex HD grade 2, Genotype = 17/45, Age = 42, sex = M


title: 91 FC BA9  A

description: human frontal cortex

tag: 91 FC human frontal cortex control, Genotype = 18/19, Age = 61, sex = F


title: 88 FC BA9  A

description: human frontal cortex

tag: 88 FC human frontal cortex control, Genotype = 13/17, Age = 68, sex = F


title: 90 FC BA9  A

description: human frontal cortex

tag: 90 FC human frontal cortex HD grade 3, Genotype = 20/49, Age = 52, sex = M


title: 89 FC BA9  A

description: human frontal cortex

tag: 89 FC human frontal cortex HD grade 3, Genotype = 21/45, Age = 56, sex = F


title: 95 FC BA9  A

description: human frontal cortex

tag: 95 FC human frontal cortex HD grade 4, Genotype = 19/43, Age = 68, sex = M


title: H120 FC BA4  B

description: human frontal cortex

tag: H120 FC, human frontal cortex control, Genotype = 18/22, Age = 62, sex = M


title: HC102 FC BA4  B

description: human frontal cortex

tag: HC102 FC, human frontal cortex HD grade 3, Genotype = 17/42, Age = 65, sex = M


title: H121 FC BA4  B

description: human frontal cortex

tag: H121 FC, human frontal cortex control, Genotype = 18/23, Age = 64, sex = F


title: H129 FC BA4  B

description: human frontal cortex

tag: H129 FC, human frontal cortex control, Genotype = 20/21, Age = 48, sex = M


title: 86 FC BA9  A

description: human frontal cortex

tag: 86 FC human frontal cortex control, Genotype = 17/11, Age = 51, sex = M


title: HC73 FC BA4  A

description: human frontal cortex

tag: HC73 FC human frontal cortex HD grade 2, Genotype = 19/49, Age = 47, sex = M


title: H117 FC BA4  B

description: human frontal cortex

tag: H117 FC, human frontal cortex control, Genotype = 17/17, Age = 64, sex = M


title: 84 FC BA9  A

description: human frontal cortex

tag: 84 FC human frontal cortex HD grade 2, Genotype = 17/44, Age = 57, sex = M


title: 123 FC BA9  A

description: human frontal cortex

tag: 123 FC human frontal cortex HD grade 1, Genotype = 18/45, Age = 64, sex = M


title: H113 FC BA4  B

description: human frontal cortex

tag: H113 FC, human frontal cortex control, Genotype = 10/17, Age = 68, sex = M


title: 121 FC BA9  A

description: human frontal cortex

tag: 121 FC human frontal cortex control, Genotype = 17/17, Age = 22, sex = M


title: H118 FC BA4  B

description: human frontal cortex

tag: H118 FC, human frontal cortex control, Genotype = 15/16, Age = 57, sex = M


title: H123 FC BA4  B

description: human frontal cortex

tag: H123 FC, human frontal cortex control, Genotype = 17/20, Age = 78, sex = M


title: 120 FC BA9  A

description: human frontal cortex

tag: 120 FC human frontal cortex control, Genotype = 18/23, Age = 67, sex = M


title: 118 FC BA9  A

description: human frontal cortex

tag: 118 FC human frontal cortex control, Genotype = 16/24, Age = 29, sex = M


title: 124 FC BA9  A

description: human frontal cortex

tag: 124 FC human frontal cortex HD grade 1, Genotype = 20/41, Age = 69, sex = F


title: 119 FC BA9  A

description: human frontal cortex

tag: 119 FC human frontal cortex HD grade 2, Genotype = 27/42, Age = 70, sex = M


title: H109 FC BA4  B

description: human frontal cortex

tag: H109 FC, human frontal cortex control, Genotype = 15/18, Age = 81, sex = M


title: 96 FC BA9  B

description: human frontal cortex

tag: 96 FC, human frontal cortex HD grade 3, Genotype = 17/49, Age = 51, sex = M


title: 97 FC BA9  B

description: human frontal cortex

tag: 97 FC, human frontal cortex HD grade 2, Genotype = 17/45, Age = 42, sex = M


title: H115 FC BA4  B

description: human frontal cortex

tag: H115 FC, human frontal cortex control, Genotype = 17/19, Age = 61, sex = M


title: 113 FC BA9  A

description: human frontal cortex

tag: 113 FC human frontal cortex HD grade 4, Genotype = 20/48, Age = 37, sex = M


title: 114 FC BA9  A

description: human frontal cortex

tag: 114 FC human frontal cortex control, Genotype = 18/20, Age = 72, sex = M


title: 115 FC BA9  A

description: human frontal cortex

tag: 115 FC human frontal cortex control, Genotype = 16/20, Age = 81, sex = M


title: 92 FC BA9  B

description: human frontal cortex

tag: 92 FC, human frontal cortex HD grade 0, Genotype = 17/43, Age = 32, sex = F


title: 112 FC BA9  A

description: human frontal cortex

tag: 112 FC human frontal cortex HD grade 0, Genotype = 16/42, Age = 40, sex = M


title: 99 FC BA9  B

description: human frontal cortex

tag: 99 FC, human frontal cortex HD grade 4, Genotype = 15/48, Age = 42, sex = F


title: 90 FC BA9  B

description: human frontal cortex

tag: 90 FC, human frontal cortex HD grade 3, Genotype = 20/49, Age = 52, sex = M


title: 111 FC BA9  A

description: human frontal cortex

tag: 111 FC human frontal cortex HD grade 4, Genotype = 17/44, Age = 59, sex = M


title: 88 FC BA9  B

description: human frontal cortex

tag: 88 FC, human frontal cortex control, Genotype = 13/17, Age = 68, sex = F


title: 91 FC BA9  B

description: human frontal cortex

tag: 91 FC, human frontal cortex control, Genotype = 18/19, Age = 61, sex = F


title: 116 FC BA9  A

description: human frontal cortex

tag: 116 FC human frontal cortex control, Genotype = 17/23, Age = 68, sex = F


title: 86 FC BA9  B

description: human frontal cortex

tag: 86 FC, human frontal cortex control, Genotype = 17/11, Age = 51, sex = M


title: 110 FC BA9  A

description: human frontal cortex

tag: 110 FC human frontal cortex control, Genotype = 17/19, Age = 37, sex = M


title: 107 FC BA9  A

description: human frontal cortex

tag: 107 FC human frontal cortex HD grade 1, Genotype = 19/46, Age = 19, sex = M


title: 108 FC BA9  A

description: human frontal cortex

tag: 108 FC human frontal cortex control, Genotype = 16/18, Age = 22, sex = M


title: H111 FC BA4  B

description: human frontal cortex

tag: H111 FC, human frontal cortex control, Genotype = 17/20, Age = 46, sex = M


title: 121 FC BA9  B

description: human frontal cortex

tag: 121 FC, human frontal cortex control, Genotype = 17/17, Age = 22, sex = M


title: 84 FC BA9  B

description: human frontal cortex

tag: 84 FC, human frontal cortex HD grade 2, Genotype = 17/44, Age = 57, sex = M


title: 123 FC BA9  B

description: human frontal cortex

tag: 123 FC, human frontal cortex HD grade 1, Genotype = 18/45, Age = 64, sex = M


title: 124 FC BA9  B

description: human frontal cortex

tag: 124 FC, human frontal cortex HD grade 1, Genotype = 20/41, Age = 69, sex = F


title: 109 FC BA9  A

description: human frontal cortex

tag: 109 FC human frontal cortex HD grade 1, Genotype = 16/45, Age = 34, sex = F


title: 106 FC BA9  A

description: human frontal cortex

tag: 106 FC human frontal cortex control, Genotype = 18/20, Age = 37, sex = F


title: 100 FC BA9  A

description: human frontal cortex

tag: 100 FC human frontal cortex HD grade 2, Genotype = 23/38, Age = 87, sex = M


title: 103 FC BA9  A

description: human frontal cortex

tag: 103 FC human frontal cortex HD grade 2, Genotype = 18/43, Age = 65, sex = M


title: HC86 CN A

description: human caudate nucleus

source_name: Caudate Nucleus

tag: HC86 CN, human caudate nucleus HD grade 1, Genotype = 17/37, Age = 46, sex = M


title: 119 FC BA9  B

description: human frontal cortex

tag: 119 FC, human frontal cortex HD grade 2, Genotype = 27/42, Age = 70, sex = M


title: HC83 CN A

description: human caudate nucleus

tag: HC83 CN, human caudate nucleus HD grade 1, Genotype = 20/40, Age = 80, sex = M


title: HC82 CN A

description: human caudate nucleus

tag: HC82 CN, human caudate nucleus HD grade 2, Genotype = 15/42, Age = 74, sex = M


title: 116 FC BA9  B

description: human frontal cortex

tag: 116 FC, human frontal cortex control, Genotype = 17/23, Age = 68, sex = F


title: 118 FC BA9  B

description: human frontal cortex

tag: 118 FC, human frontal cortex control, Genotype = 16/24, Age = 29, sex = M


title: 120 FC BA9  B

description: human frontal cortex

tag: 120 FC, human frontal cortex control, Genotype = 18/23, Age = 67, sex = M


title: HC76 CN A

description: human caudate nucleus

tag: HC76 CN, human caudate nucleus HD grade 2, Genotype = 19/42, Age = 71, sex = M


title: 115 FC BA9  B

description: human frontal cortex

tag: 115 FC, human frontal cortex control, Genotype = 16/20, Age = 81, sex = M


title: HC81 CN A

description: human caudate nucleus

tag: HC81 CN, human caudate nucleus HD grade 1, Genotype = 19/41, Age = 70, sex = F


title: HC80 CN A

description: human caudate nucleus

tag: HC80 CN, human caudate nucleus HD grade 2, Genotype = 23/43, Age = 45, sex = F


title: H104 FC BA4  B

description: human frontal cortex

tag: H104 FC, human frontal cortex control, Genotype = 18/25, Age = 69, sex = M


title: 112 FC BA9  B

description: human frontal cortex

tag: 112 FC, human frontal cortex HD grade 0, Genotype = 16/42, Age = 40, sex = M


title: 114 FC BA9  B

description: human frontal cortex

tag: 114 FC, human frontal cortex control, Genotype = 18/20, Age = 72, sex = M


title: 113 FC BA9  B

description: human frontal cortex

tag: 113 FC, human frontal cortex HD grade 4, Genotype = 20/48, Age = 37, sex = M


title: HC72 CN A

description: human caudate nucleus

tag: HC72 CN, human caudate nucleus HD grade 2, Genotype = 17/42, Age = 63, sex = F


title: HC71 CN A

description: human caudate nucleus

tag: HC71 CN, human caudate nucleus HD grade 0, Genotype = 17/40, Age = 70, sex = F


title: 111 FC BA9  B

description: human frontal cortex

tag: 111 FC, human frontal cortex HD grade 4, Genotype = 17/44, Age = 59, sex = M


title: 110 FC BA9  B

description: human frontal cortex

tag: 110 FC, human frontal cortex control, Genotype = 17/19, Age = 37, sex = M


title: HC68 CN A

description: human caudate nucleus

tag: HC68 CN, human caudate nucleus HD grade 1, Genotype = 17/42, Age = 65, sex = F


title: HC69 CN A

description: human caudate nucleus

tag: HC69 CN, human caudate nucleus HD grade 2, Genotype = 16/46, Age = 50, sex = F


title: HC74 CN A

description: human caudate nucleus

tag: HC74 CN, human caudate nucleus HD grade 1, Genotype = 17/42, Age = 42, sex = F


title: HC73 CN A

description: human caudate nucleus

tag: HC73 CN, human caudate nucleus HD grade 2, Genotype = 19/49, Age = 47, sex = M


title: 109 FC BA9  B

description: human frontal cortex

tag: 109 FC, human frontal cortex HD grade 1, Genotype = 16/45, Age = 34, sex = F


title: 106 FC BA9  B

description: human frontal cortex

tag: 106 FC, human frontal cortex control, Genotype = 18/20, Age = 37, sex = F


title: 108 FC BA9  B

description: human frontal cortex

tag: 108 FC, human frontal cortex control, Genotype = 16/18, Age = 22, sex = M


title: HC61 CN A

description: human caudate nucleus

tag: HC61 CN, human caudate nucleus HD grade 2, Genotype = 17/46, Age = 65, sex = M


title: 103 FC BA9  B

description: human frontal cortex

tag: 103 FC, human frontal cortex HD grade 2, Genotype = 18/43, Age = 65, sex = M


title: 100 FC BA9  B

description: human frontal cortex

tag: 100 FC, human frontal cortex HD grade 2, Genotype = 23/38, Age = 87, sex = M


title: HC55 CN A

description: human caudate nucleus

tag: HC55 CN, human caudate nucleus HD grade 1, Genotype = 14/42, Age = 87, sex = M


title: HC57 CN A

description: human caudate nucleus

tag: HC57 CN, human caudate nucleus HD grade 2, Genotype = 18/47, Age = 58, sex = M


title: HC52 CN A

description: human caudate nucleus

tag: HC52 CN, human caudate nucleus HD grade 2, Genotype = 19/46, Age = 61, sex = F


title: HC62 CN A

description: human caudate nucleus

tag: HC62 CN, human caudate nucleus HD grade 2, Genotype = 18/43, Age = 78, sex = F


title: HC65 CN A

description: human caudate nucleus

tag: HC65 CN, human caudate nucleus HD grade 2, Genotype = 18/42, Age = 82, sex = F


title: HC66 CN A

description: human caudate nucleus

tag: HC66 CN, human caudate nucleus HD grade 0, Genotype = 27/41, Age = 62, sex = M


title: HC83 CN B

description: human caudate nucleus

tag: HC83 CN human caudate nucleus HD grade 1, Genotype = 20/40, Age = 80, sex = M


title: HC81 CN B

description: human caudate nucleus

tag: HC81 CN human caudate nucleus HD grade 1, Genotype = 19/41, Age = 70, sex = F


title: 92 FC BA9  A

description: human frontal cortex

tag: 92 FC human frontal cortex HD grade 0, Genotype = 17/43, Age = 32, sex = F


title: HC105 CN A

description: human caudate nucleus

tag: HC105 CN, human caudate nucleus HD grade 1, Genotype = 15/42, Age = 67, sex = F


title: HC80 CN B

description: human caudate nucleus

tag: HC80 CN human caudate nucleus HD grade 2, Genotype = 23/43, Age = 45, sex = F


title: HC82 CN B

description: human caudate nucleus

tag: HC82 CN human caudate nucleus HD grade 2, Genotype = 15/42, Age = 74, sex = M


title: HC53 CN A

description: human caudate nucleus

tag: HC53 CN, human caudate nucleus HD grade 1, Genotype = 17/43, Age = 56, sex = M


title: HC86 CN B

description: human caudate nucleus

tag: HC86 CN human caudate nucleus HD grade 1, Genotype = 17/37, Age = 46, sex = M


title: HC103 CN A

description: human caudate nucleus

tag: HC103 CN, human caudate nucleus HD grade 1, Genotype = 19/39, Age = 41, sex = M


title: H85 CN A

description: human caudate nucleus

tag: H85 CN, human caudate nucleus control, Genotype = 18/18, Age = 94, sex = M


title: HC72 CN B

description: human caudate nucleus

tag: HC72 CN human caudate nucleus HD grade 2, Genotype = 17/42, Age = 63, sex = F


title: HC74 CN B

description: human caudate nucleus

tag: HC74 CN human caudate nucleus HD grade 1, Genotype = 17/42, Age = 42, sex = F


title: HC102 CN A

description: human caudate nucleus

tag: HC102 CN, human caudate nucleus HD grade 3, Genotype = 17/42, Age = 65, sex = M


title: HC73 CN B

description: human caudate nucleus

tag: HC73 CN human caudate nucleus HD grade 2, Genotype = 19/49, Age = 47, sex = M


title: H137 CN A

description: human caudate nucleus

tag: H137 CN, human caudate nucleus control, Genotype = 18/20, Age = 77, sex = F


title: H131 CN A

description: human caudate nucleus

tag: H131 CN, human caudate nucleus control, Genotype = 17/17, Age = 73, sex = F


title: HC76 CN B

description: human caudate nucleus

tag: HC76 CN human caudate nucleus HD grade 2, Genotype = 19/42, Age = 71, sex = M


title: H126 CN A

description: human caudate nucleus

tag: H126 CN, human caudate nucleus control, Genotype = 20/22, Age = 36, sex = F


title: HC71 CN B

description: human caudate nucleus

tag: HC71 CN human caudate nucleus HD grade 0, Genotype = 17/40, Age = 70, sex = F


title: HC68 CN B

description: human caudate nucleus

tag: HC68 CN human caudate nucleus HD grade 1, Genotype = 17/42, Age = 65, sex = F


title: HC69 CN B

description: human caudate nucleus

tag: HC69 CN human caudate nucleus HD grade 2, Genotype = 16/46, Age = 50, sex = F


title: H132 CN A

description: human caudate nucleus

tag: H132 CN, human caudate nucleus control, Genotype = 15/19, Age = 63, sex = F


title: HC66 CN B

description: human caudate nucleus

tag: HC66 CN human caudate nucleus HD grade 0, Genotype = 27/41, Age = 62, sex = M


title: H128 CN A

description: human caudate nucleus

tag: H128 CN, human caudate nucleus control, Genotype = 17/19, Age = 34, sex = F


title: H124 CN A

description: human caudate nucleus

tag: H124 CN, human caudate nucleus control, Genotype = 17/17, Age = 49, sex = M


title: H129 CN A

description: human caudate nucleus

tag: H129 CN, human caudate nucleus control, Genotype = 20/21, Age = 48, sex = M


title: HC65 CN B

description: human caudate nucleus

tag: HC65 CN human caudate nucleus HD grade 2, Genotype = 18/42, Age = 82, sex = F


title: 89 FC BA9  B

description: human frontal cortex

tag: 89 FC, human frontal cortex HD grade 3, Genotype = 21/45, Age = 56, sex = F


title: HC61 CN B

description: human caudate nucleus

tag: HC61 CN human caudate nucleus HD grade 2, Genotype = 17/46, Age = 65, sex = M


title: H123 CN A

description: human caudate nucleus

tag: H123 CN, human caudate nucleus control, Genotype = 17/20, Age = 78, sex = M


title: H121 CN A

description: human caudate nucleus

tag: H121 CN, human caudate nucleus control, Genotype = 18/23, Age = 64, sex = F


title: HC57 CN B

description: human caudate nucleus

tag: HC57 CN human caudate nucleus HD grade 2, Genotype = 18/47, Age = 58, sex = M


title: HC55 CN B

description: human caudate nucleus

tag: HC55 CN human caudate nucleus HD grade 1, Genotype = 14/42, Age = 87, sex = M


title: HC53 CN B

description: human caudate nucleus

tag: HC53 CN human caudate nucleus HD grade 1, Genotype = 17/43, Age = 56, sex = M


title: HC62 CN B

description: human caudate nucleus

tag: HC62 CN human caudate nucleus HD grade 2, Genotype = 18/43, Age = 78, sex = F


title: H117 CN A

description: human caudate nucleus

tag: H117 CN, human caudate nucleus control, Genotype = 17/17, Age = 64, sex = M


title: H137 CN B

description: human caudate nucleus

tag: H137 CN human caudate nucleus control, Genotype = 18/20, Age = 77, sex = F


title: 9 CN A

description: human caudate nucleus

tag: 9 CN, human caudate nucleus HD grade 4, Genotype = 17/44, Age = 59, sex = M


title: 107 FC BA9  B

description: human frontal cortex

tag: 107 FC, human frontal cortex HD grade 1, Genotype = 19/46, Age = 19, sex = M


title: H111 CN A

description: human caudate nucleus

tag: H111 CN, human caudate nucleus control, Genotype = 17/20, Age = 46, sex = M


title: HC102 CN B

description: human caudate nucleus

tag: HC102 CN human caudate nucleus HD grade 3, Genotype = 17/42, Age = 65, sex = M


title: H120 CN A

description: human caudate nucleus

tag: H120 CN, human caudate nucleus control, Genotype = 18/22, Age = 62, sex = M


title: H115 CN A

description: human caudate nucleus

tag: H115 CN, human caudate nucleus control, Genotype = 17/19, Age = 61, sex = M


title: 95 FC BA9  B

description: human frontal cortex

tag: 95 FC, human frontal cortex HD grade 4, Genotype = 19/43, Age = 68, sex = M


title: HC52 CN B

description: human caudate nucleus

tag: HC52 CN human caudate nucleus HD grade 2, Genotype = 19/46, Age = 61, sex = F


title: H113 CN A

description: human caudate nucleus

tag: H113 CN, human caudate nucleus control, Genotype = 10/17, Age = 68, sex = M


title: H109 CN A

description: human caudate nucleus

tag: H109 CN, human caudate nucleus control, Genotype = 15/18, Age = 81, sex = M


title: HC105 CN B

description: human caudate nucleus

tag: HC105 CN human caudate nucleus HD grade 1, Genotype = 15/42, Age = 67, sex = F


title: H118 CN A

description: human caudate nucleus

tag: H118 CN, human caudate nucleus control, Genotype = 15/16, Age = 57, sex = M


title: H132 CN B

description: human caudate nucleus

tag: H132 CN human caudate nucleus control, Genotype = 15/19, Age = 63, sex = F


title: H85 CN B

description: human caudate nucleus

tag: H85 CN human caudate nucleus control, Genotype = 18/18, Age = 94, sex = M


title: HC103 CN B

description: human caudate nucleus

tag: HC103 CN human caudate nucleus HD grade 1, Genotype = 19/39, Age = 41, sex = M


title: 8 CN A

description: human caudate nucleus

tag: 8 CN, human caudate nucleus control, Genotype = 18/20, Age = 37, sex = F


title: 7 CN A

description: human caudate nucleus

tag: 7 CN, human caudate nucleus HD grade 1, Genotype = 19/46, Age = 19, sex = M


title: H128 CN B

description: human caudate nucleus

tag: H128 CN human caudate nucleus control, Genotype = 17/19, Age = 34, sex = F


title: H104 CN A

description: human caudate nucleus

tag: H104 CN, human caudate nucleus control, Genotype = 18/25, Age = 69, sex = M


title: 64 CN A

description: human caudate nucleus

tag: 64 CN, human caudate nucleus control, Genotype = 17/11, Age = 51, sex = M


title: 62 CN A

description: human caudate nucleus

tag: 62 CN, human caudate nucleus HD grade 3, Genotype = 21/45, Age = 56, sex = F


title: H121 CN B

description: human caudate nucleus

tag: H121 CN human caudate nucleus control, Genotype = 18/23, Age = 64, sex = F


title: H123 CN B

description: human caudate nucleus

tag: H123 CN human caudate nucleus control, Genotype = 17/20, Age = 78, sex = M


title: 60 CN A

description: human caudate nucleus

tag: 60 CN, human caudate nucleus HD grade 3, Genotype = 17/49, Age = 51, sex = M


title: 52 CN A

description: human caudate nucleus

tag: 52 CN, human caudate nucleus control, Genotype = 15/20, Age = 55, sex = F


title: 51 CN A

description: human caudate nucleus

tag: 51 CN, human caudate nucleus HD grade 2, Genotype = 17/45, Age = 42, sex = M


title: 59 CN A

description: human caudate nucleus

tag: 59 CN, human caudate nucleus HD grade 2, Genotype = 17/44, Age = 57, sex = M


title: 22 CN A

description: human caudate nucleus

tag: 22 CN, human caudate nucleus HD grade 2, Genotype = 18/43, Age = 65, sex = M


title: H118 CN B

description: human caudate nucleus

tag: H118 CN human caudate nucleus control, Genotype = 15/16, Age = 57, sex = M


title: H109 CN B

description: human caudate nucleus

tag: H109 CN human caudate nucleus control, Genotype = 15/18, Age = 81, sex = M


title: 21 CN A

description: human caudate nucleus

tag: 21 CN, human caudate nucleus control, Genotype = 17/23, Age = 68, sex = F


title: H117 CN B

description: human caudate nucleus

tag: H117 CN human caudate nucleus control, Genotype = 17/17, Age = 64, sex = M


title: H115 CN B

description: human caudate nucleus

tag: H115 CN human caudate nucleus control, Genotype = 17/19, Age = 61, sex = M


title: 45 CN A

description: human caudate nucleus

tag: 45 CN, human caudate nucleus HD grade 3, Genotype = 20/49, Age = 52, sex = M


title: H111 CN B

description: human caudate nucleus

tag: H111 CN human caudate nucleus control, Genotype = 17/20, Age = 46, sex = M


title: H113 CN B

description: human caudate nucleus

tag: H113 CN human caudate nucleus control, Genotype = 10/17, Age = 68, sex = M


title: 3 CN A

description: human caudate nucleus

tag: 3 CN, human caudate nucleus HD grade 1, Genotype = 16/45, Age = 34, sex = F


title: H120 CN B

description: human caudate nucleus

tag: H120 CN human caudate nucleus control, Genotype = 18/22, Age = 62, sex = M


title: 19 CN A

description: human caudate nucleus

tag: 19 CN, human caudate nucleus HD grade 2, Genotype = 23/41, Age = 79, sex = F


title: 2 CN A

description: human caudate nucleus

tag: 2 CN, human caudate nucleus control, Genotype = 16/18, Age = 22, sex = M


title: 20 CN A

description: human caudate nucleus

tag: 20 CN, human caudate nucleus control, Genotype = 18/23, Age = 67, sex = M


title: H124 CN B

description: human caudate nucleus

tag: H124 CN human caudate nucleus control, Genotype = 17/17, Age = 49, sex = M


title: H129 CN B

description: human caudate nucleus

tag: H129 CN human caudate nucleus control, Genotype = 20/21, Age = 48, sex = M


title: H104 CN B

description: human caudate nucleus

tag: H104 CN human caudate nucleus control, Genotype = 18/25, Age = 69, sex = M


title: H131 CN B

description: human caudate nucleus

tag: H131 CN human caudate nucleus control, Genotype = 17/17, Age = 73, sex = F


title: 18 CN A

description: human caudate nucleus

tag: 18 CN, human caudate nucleus control, Genotype = 16/20, Age = 81, sex = M


title: 62 CN B

description: human caudate nucleus

tag: 62 CN human caudate nucleus HD grade 3, Genotype = 21/45, Age = 56, sex = F


title: 7 CN B

description: human caudate nucleus

tag: 7 CN human caudate nucleus HD grade 1, Genotype = 19/46, Age = 19, sex = M


title: 9 CN B

description: human caudate nucleus

tag: 9 CN human caudate nucleus HD grade 4, Genotype = 17/44, Age = 59, sex = M


title: 59 CN B

description: human caudate nucleus

tag: 59 CN human caudate nucleus HD grade 2, Genotype = 17/44, Age = 57, sex = M


title: 8 CN B

description: human caudate nucleus

tag: 8 CN human caudate nucleus control, Genotype = 18/20, Age = 37, sex = F


title: 64 CN B

description: human caudate nucleus

tag: 64 CN human caudate nucleus control, Genotype = 17/11, Age = 51, sex = M


title: 126 CN A

description: human caudate nucleus

tag: 126 CN, human caudate nucleus control, Genotype = 17/17, Age = 75, sex = M


title: 52 CN B

description: human caudate nucleus

tag: 52 CN human caudate nucleus control, Genotype = 15/20, Age = 55, sex = F


title: 11 CN A

description: human caudate nucleus

tag: 11 CN, human caudate nucleus control, Genotype = 16/24, Age = 29, sex = M


title: 12 CN A

description: human caudate nucleus

tag: 12 CN, human caudate nucleus HD grade 1, Genotype = 18/45, Age = 64, sex = M


title: 15 CN A

description: human caudate nucleus

tag: 15 CN, human caudate nucleus control, Genotype = 17/19, Age = 60, sex = M


title: 16 CN A

description: human caudate nucleus

tag: 16 CN, human caudate nucleus HD grade 0, Genotype = 16/42, Age = 40, sex = M


title: 17 CN A

description: human caudate nucleus

tag: 17 CN, human caudate nucleus control, Genotype = 18/20, Age = 72, sex = M


title: 13 CN A

description: human caudate nucleus

tag: 13 CN, human caudate nucleus HD grade 1, Genotype = 20/41, Age = 69, sex = F


title: 14 CN A

description: human caudate nucleus

tag: 14 CN, human caudate nucleus control, Genotype = 17/17, Age = 22, sex = M


title: 101 CN A

description: human caudate nucleus

tag: 101 CN, human caudate nucleus control, Genotype = 9/17, Age = 68, sex = M


title: 10 CN A

description: human caudate nucleus

tag: 10 CN, human caudate nucleus HD grade 2, Genotype = 27/42, Age = 70, sex = M


title: 45 CN B

description: human caudate nucleus

tag: 45 CN human caudate nucleus HD grade 3, Genotype = 20/49, Age = 52, sex = M


title: 3 CN B

description: human caudate nucleus

tag: 3 CN human caudate nucleus HD grade 1, Genotype = 16/45, Age = 34, sex = F


title: 51 CN B

description: human caudate nucleus

tag: 51 CN human caudate nucleus HD grade 2, Genotype = 17/45, Age = 42, sex = M


title: 60 CN B

description: human caudate nucleus

tag: 60 CN human caudate nucleus HD grade 3, Genotype = 17/49, Age = 51, sex = M


title: 22 CN B

description: human caudate nucleus

tag: 22 CN human caudate nucleus HD grade 2, Genotype = 18/43, Age = 65, sex = M


title: 102 CN A

description: human caudate nucleus

tag: 102 CN, human caudate nucleus HD grade 3, Genotype = 15/55, Age = 40, sex = M


title: 21 CN B

description: human caudate nucleus

tag: 21 CN human caudate nucleus control, Genotype = 17/23, Age = 68, sex = F


title: 19 CN B

description: human caudate nucleus

tag: 19 CN human caudate nucleus HD grade 2, Genotype = 23/41, Age = 79, sex = F


title: 2 CN B

description: human caudate nucleus

tag: 2 CN human caudate nucleus control, Genotype = 16/18, Age = 22, sex = M


title: 12 h-apra-10 nM;HT-29;colorectal adenocarcinoma;apratox;24730

description: 12 h-apra-10 nM

data_processing: Rosetta Resolver intensity value

tag: 12 h-apra-10 nM


title: 18 CN B

description: human caudate nucleus

tag: 18 CN human caudate nucleus control, Genotype = 16/20, Age = 81, sex = M


title: H126 CN B

description: human caudate nucleus

tag: H126 CN human caudate nucleus control, Genotype = 20/22, Age = 36, sex = F


title: 16 CN B

description: human caudate nucleus

tag: 16 CN human caudate nucleus HD grade 0, Genotype = 16/42, Age = 40, sex = M


title: 17 CN B

description: human caudate nucleus

tag: 17 CN human caudate nucleus control, Genotype = 18/20, Age = 72, sex = M


title: 20 CN B

description: human caudate nucleus

tag: 20 CN human caudate nucleus control, Genotype = 18/23, Age = 67, sex = M


title: 12 h-apra-2 nM;HT-29;colorectal adenocarcinoma;apratoxi;24728

description: 12 h-apra-10 nM


title: 12 h-apra-10 nM;HT-29;colorectal adenocarcinoma;apratox;24729

description: 12 h-apra-10 nM


title: 12 h-control (EtOH);HT-29;colorectal adenocarcinoma;Eth;24725

description: 12 h-control (EtOH)

tag: 12 h-control (EtOH)


title: 12 h-apra-2 nM;HT-29;colorectal adenocarcinoma;apratoxi;24727

description: 12 h-apra-2 nM

tag: 12 h-apra-2 nM


title: 15 CN B

description: human caudate nucleus

tag: 15 CN human caudate nucleus control, Genotype = 17/19, Age = 60, sex = M


title: 6 h-apra-10 nM;HT-29;colorectal adenocarcinoma;apratoxi;24723

description: 6 h-apra-10 nM

tag: 6 h-apra-10 nM


title: 6 h-apra-2 nM;HT-29;colorectal adenocarcinoma;apratoxin;24722

description: 6 h-apra-2 nM

tag: 6 h-apra-2 nM


title: 1 CN A

description: human caudate nucleus

tag: 1 CN, human caudate nucleus control, Genotype = 17/19, Age = 37, sex = M


title: 14 CN B

description: human caudate nucleus

tag: 14 CN human caudate nucleus control, Genotype = 17/17, Age = 22, sex = M


title: 12 h-control (EtOH);HT-29;colorectal adenocarcinoma;Eth;24726

description: 12 h-control (EtOH)


title: 6 h-apra-10 nM;HT-29;colorectal adenocarcinoma;apratoxi;24724

description: 6 h-apra-10 nM


title: 126 CN B

description: human caudate nucleus

tag: 126 CN human caudate nucleus control, Genotype = 17/17, Age = 75, sex = M


title: 6 h-control (EtOH);HT-29;colorectal adenocarcinoma;Etha;24719

description: 6 h-control (EtOH)

tag: 6 h-control (EtOH)


title: 13 CN B

description: human caudate nucleus

tag: 13 CN human caudate nucleus HD grade 1, Genotype = 20/41, Age = 69, sex = F


title: 12 CN B

description: human caudate nucleus

tag: 12 CN human caudate nucleus HD grade 1, Genotype = 18/45, Age = 64, sex = M


title: 6 h-apra-2 nM;HT-29;colorectal adenocarcinoma;apratoxin;24721

description: 6 h-apra-2 nM


title: 3 h-apra-10 nM;HT-29;colorectal adenocarcinoma;apratoxi;24718

description: 3 h-apra-10 nM

tag: 3 h-apra-10 nM


title: 6 h-control (EtOH);HT-29;colorectal adenocarcinoma;Etha;24720

description: 6 h-control (EtOH)


title: 3 h-apra-2 nM;HT-29;colorectal adenocarcinoma;apratoxin;24716

description: 3 h-apra-2 nM

tag: 3 h-apra-2 nM


title: 3 h-apra-2 nM;HT-29;colorectal adenocarcinoma;apratoxin;24715

description: 3 h-apra-2 nM


title: 101 CN B

description: human caudate nucleus

tag: 101 CN human caudate nucleus control, Genotype = 9/17, Age = 68, sex = M


title: 3 h-apra-10 nM;HT-29;colorectal adenocarcinoma;apratoxi;24717

description: 3 h-apra-10 nM


title: 10 CN B

description: human caudate nucleus

tag: 10 CN human caudate nucleus HD grade 2, Genotype = 27/42, Age = 70, sex = M


title: 3 h-control (EtOH);HT-29;colorectal adenocarcinoma;Etha;24713

description: 3 h-control (EtOH)

tag: 3 h-control (EtOH)


title: 1 CN B

description: human caudate nucleus

tag: 1 CN human caudate nucleus control, Genotype = 17/19, Age = 37, sex = M


title: Tbk1+/+ Ikbke-/- B-DNA transfection

status: Public on Feb 04 2006

city: Suita

first: Shizuo

last: Akira

state: Osaka

address: 3-1 Yamada-oka

department: Department of Host Defense

institute: Reserch Institute for Microbial Diseases, Osaka University

description: Tbk1+/+ Ikbke-/-  4h after B-DNA transfection

data_processing: MAS5.0, scaled to 500

label: PE

source_name: Embryonic fibroblasts

tag: Strain

value: C57BL/6 x 129Ola

tag: Cell

value: Embryonic fibroblats


title: 3 h-control (EtOH);HT-29;colorectal adenocarcinoma;Etha;24714

description: 3 h-apra-2 nM


title: Tbk1-/- Ikbke-/- mock transfection

description: Tbk1-/- Ikbke-/-  4h after mock transfection

value: Embryonic fibroblasts


title: 11 CN B

description: human caudate nucleus

tag: 11 CN human caudate nucleus control, Genotype = 16/24, Age = 29, sex = M


title: Tbk1-/- Ikbke-/- B-DNA transfection

description: Tbk1-/- Ikbke-/-  4h after B-DNA transfection

value: embryonic fibroblasts


title: Tbk1-/-_mock transfection

description: Tbk1-/- Ikbke+/+ 4h after mock transfection


title: 102 CN B

description: human caudate nucleus

tag: 102 CN human caudate nucleus HD grade 3, Genotype = 15/55, Age = 40, sex = M


title: Tbk1+/+ Ikbke-/- mock transfection

description: Tbk1+/+ Ikbke-/-  4h after mock transfection


title: WT_B-DNA transfection

description: Tbk1+/+ Ikbke+/+ 4h after B-DNA transfection


title: Tbk1-/- Ikbke+/+ B-DNA transfection

description: Tbk1-/- Ikbke+/+ 4h after B-DNA transfection


title: adult head_CT2_experimentywtim2

status: Public on Feb 03 2006

city: Southampton

first: Herman

last: Wijnen

state: Hampshire

address: Life Sciences Building 85 - Highfield Campus

department: Centre for Biological Sciences

institute: University of Southampton

data_processing: Bioconductor Robust Multi-array Average

organism: Drosophila melanogaster

source_name: adult head

label_protocol: Approximately 25 µg of total RNA was processed to produce biotinylated cRNA targets.

extract_protocol: Flies were immediately frozen on dry ice. Approximately 100-200 heads were isolated on sieves after breaking the frozen flies

tag: y w; tim01 arrhythmic mutant stock, adult head


title: adult head_CT10_experimentywtim2


title: adult head_CT14_experimentywtim2


title: adult head_CT6_experimentywtim2


title: WT mock transfection

description: Tbk1+/+ Ikbke+/+ 4h after mock transfection


title: adult head_CT22_experimentywtim2


title: adult head_ZT6_experimentywtim1


title: adult head_ZT18_experimentywtim2


title: adult head_CT2_experimentywtim1


title: adult head_CT6_experimentywtim1


title: adult head_ZT22_experimentywtim2


title: adult head_ZT2_experimentywtim1


title: adult head_ZT10_experimentywtim2


title: adult head_CT22_experimentyw3

tag: y w 'wild type' stock, adult head


title: adult head_ZT14_experimentywtim2


title: adult head_ZT2_experimentywtim2


title: adult head_ZT6_experimentywtim2


title: adult head_CT22_experimentywtim1


title: adult head_CT18_experimentywtim1


title: adult head_ZT10_experimentywtim1


title: adult head_CT6_experimentyw3


title: adult head_ZT14_experimentywtim1


title: adult head_ZT22_experimentywtim1


title: adult head_CT10_experimentywtim1


title: adult head_ZT18_experimentywtim1


title: adult head_CT14_experimentywtim1


title: adult head_ZT22_experimentyw3


title: adult head_ZT6_experimentyw3


title: adult head_CT24_experimentcnbw2

tag: cn bw 'wild type' stock, adult head


title: adult head_CT4_experimentcnbw2


title: adult head_CT20_experimentcnbw2


title: adult head_CT18_experimentyw3


title: adult head_ZT24_experimentcnbw2


title: adult head_ZT18_experimentyw3


title: adult head_CT14_experimentyw3


title: adult head_CT10_experimentyw3


title: adult head_ZT14_experimentyw3


title: adult head_ZT10_experimentyw3


title: adult head_ZT2_experimentyw3


title: adult head_CT2_experimentyw3


title: adult head_CT16_experimentyw2


title: adult head_CT16_experimentcnbw2


title: adult head_ZT16_experimentcnbw2


title: adult head_CT8_experimentyw2


title: adult head_CT8_experimentcnbw2


title: adult head_CT4_experimentyw2


title: adult head_CT12_experimentcnbw2


title: adult head_CT12_experimentyw2


title: adult head_ZT4_experimentcnbw2


title: adult head_CT18_experimentywtim2


title: adult head_ZT20_experimentyw2


title: adult head_ZT20_experimentcnbw2


title: adult head_ZT8_experimentcnbw2


title: adult head_ZT16_experimentyw2


title: adult head_CT20_experimentyw2


title: adult head_ZT12_experimentyw2


title: adult head_CT14_experimentyw1


title: adult head_ZT12_experimentcnbw2


title: adult head_ZT4_experimentyw2


title: adult head_CT22_experimentyw1


title: adult head_CT24_experimentyw2


title: adult head_ZT24_experimentyw2


title: adult head_ZT8_experimentyw2


title: adult head_CT2_experimentyw1


title: adult head_ZT10_experimentyw1


title: adult head_CT18_experimentyw1


title: adult head_ZT22_experimentyw1


title: adult head_ZT14_experimentyw1


title: adult head_CT6_experimentyw1


title: adult head_ZT2_experimentyw1


title: adult head_CT10_experimentyw1


title: adult head_ZT18_experimentyw1


title: S5 control Cy5 AA Cy3

status: Public on Feb 02 2006

city: East Lansing

first: Jianping

last: Yu

state: MI

address: Michigan State University

department: Plant Research Laboratory

institute: Michigan State University

description: Compare antimycin A treated versus control treated S5 leaves

data_processing: Cy3 channel was normalised as published in Schaffer et al., 2001. Plant Cell 13: 113-123, PMID: 11158533, http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=11158533

molecule: polyA RNA

organism: Arabidopsis thaliana

source_name: Leaves

tag: Alternative oxidase overexpresser line S5 leaves from 5-weeks old plants, soaked in control medium for 2 hours.

tag: Alternative oxidase overexpresser line S5 leaves from 5-weeks old plants, soaked in 10 micromolar antimycin A for 2 hours.


title: adult head_ZT6_experimentyw1


title: E3.5 blastocyst 114

city: Bunkyo-ku

first: Shin

last: Kobayashi

state: Tokyo

address: 1-5-45 yushima

department: Medical Research Institute

institute: Tokyo Mdical & Dental University

description: Total RNAs were extracted from over 200 male or female sex-separated blastocysts using an ISOGEN in the presence of 1µg of yeast tRNA as a carrier.

hyb_protocol: Hybridization was carried out for 17 hours using an Agilent’s in situ Hybridization Kit Plus.

scan_protocol: All microarray experiments were carried out following the Agilent’s

data_processing: Hybridized slides were scanned using the Agilen’ts microarray scanner

label_protocol: Total RNA was labeled using a Low Input Fluorescent Linear Amplification kit (Agilent) following the manufacturer’s instruction.

growth_protocol: B6C3F1 female mice of 8 weeks in age, were superovulated with 5 IU of pregnant mare serum gonadotropin (PMS) followed by 5 IU of human chorionic gonadotropin (hCG) 48 hr later, and were mated with XGFPY male mice.  Four-cell stage embryos were collected from the oviducts 55 hr after the hCG injection and placed in kSOM and incubated in a humidified atmosphere of 5% CO2 in air at 37 ºC for an additional 38 hr.

extract_protocol: RNA was obtained using ISOGEN (NIPPON GENE Inc., Japan) which is based on the acid guanidine thiocyanate-phenol-chloroform extraction method.

treatment_protocol: No treatment

tag: Gender

value: female

tag: Age

tag: Tissue

value: whole embryo


title: E3.5 blastocyst 101


title: E3.5 blastocyst 115


title: E3.5 blastocyst 95


title: E3.5 blastocyst 96

scan_protocol: All microarray experiments were carried out following the Agilent’s standard methodology. All experimental procedures can be obtained from the Agilent’s web site (http://www.chem.agilent.com/scripts/generic.asp? lpage=11617&indcol=N&prodcol=Y).


title: Wild type control Cy3 Antimycin A Cy5

description: Compare antimycin A treated versus control treated wild type leaves

tag: Wild type gl1 leaves from 5-weeks old plants, soaked in 10 micromolar antimycin A for 2 hours.

tag: Wild type gl1 leaves from 5-weeks old plants, soaked in control medium for 2 hours.


title: Wild type control Cy5 Antimycin A Cy3

description: Compare antimycin A treated versus control treated leaves

tag: Wild type gl1 leaves from 5-week old plants, soaked in control medium for 2 hours.

tag: Wild type gl1 leaves from 5-week old plants, soaked in 10 micromolar antimycin A for 2 hours.


title: S5 control Cy3 AA Cy5


title: CGH_2005051161_Embryo2_cell4

status: Public on Feb 01 2006

type: genomic

city: Leuven

first: Joris

last: Vermeesch

address: Herestraat, 49

department: Center for Human Genetics

country: Belgium

institute: Katholic University Leuven

description: Analysis Phi29 amplified single-cell gDNA versus non-amplified gDNA

data_processing: Spot intensities were corrected for local background and only spots with signal intensities at least 1.2-fold above background were included in the analysis and log2 values were calculated. For each array, the normalised ratio was obtained by calculating the median of the log2 signal intensities over all the autosomal fragments with measurements from abnormal chromosomes masked out, and substracting this value from all the signal intensities of the spots on the array

molecule: genomic DNA

source_name: Amplified single-cell (blastomere number 4) gDNA (XY)

tag: Single-cell gDNA following Phi29 whole genome amplification

source_name: Non-amplified reference gDNA (46,XX)

tag: Reference genomic DNA


title: CGH_2005051139_Embryo2_cell5

source_name: Amplified single-cell (blastomere number 5) gDNA (XY)


title: CGH_2005051173_Embryo2_cell6

source_name: Amplified single-cell (blastomere number 6) gDNA (XY)


title: CGH_2005051145_Embryo3_cell1

source_name: Amplified single-cell (blastomere number 1) gDNA (XX)


title: CGH_2005051148_Embryo3_cell4

source_name: Amplified single-cell (blastomere number 4) gDNA (XX)


title: CGH_2005051138_Embryo2_cell1

source_name: Amplified single-cell (blastomere number 1) gDNA (XY)


title: CGH_2005051146_Embryo3_cell2

source_name: Amplified single-cell (blastomere number 2) gDNA (XX)


title: CGH_2005051149_Embryo3_cell1

source_name: Non-amplified reference gDNA (46,XY)


title: CGH_2005051147_Embryo3_cell3

source_name: Amplified single-cell (blastomere number 3) gDNA (XX)


title: wild type GFP negative rep 3C

status: Public on Jan 01 2006

city: Brookline

first: Sung

middle: Eun

last: Choe

state: MA

address: 258 Harvard St #164

institute: Wyeth Research

description: These are wild-type embryos with Twi-GAL4 and UAS-GFP insertions that target GFP fluorescence to Twist expressing cells.  Embryos were collected and aged at 25°C to 5.5-7.5 hours after egg deposition (Stage 11).  GFP-negative cells were collected using FACS.

data_processing: Bioconductors' affy package in conjunction with Choe et al.'s goldenspike package (Choe et al. Genome Biol. 2005).

source_name: nonmesodermal cells

label_protocol: Approximately 2.5-3 µg of total RNA was processed to produce biotinylated cRNA targets.

extract_protocol: The embryos were dounce homogenized into a single cell suspension. These cells were then FACS sorted. The GFP-negative cells from this sort comprise this sample.


title: CGH_2005051162_Embryo2_cell3

source_name: Amplified single-cell (blastomere number 3) gDNA (XY)


title: wild type GFP positive rep 3A

description: These are wild-type embryos with Twi-GAL4 and UAS-GFP insertions that target GFP fluorescence to Twist expressing cells.  Embryos were collected and aged at 25°C to 5.5-7.5 hours after egg deposition (Stage 11).  GFP-positive cells were collected using FACS.

source_name: mesodermal cells

extract_protocol: The embryos were dounce homogenized into a single cell suspension. These cells were then FACS sorted. The GFP-positive cells from this sort comprise this sample.


title: wild type GFP negative rep 3B

tag: These are wild-type embryos with Twi-GAL4 and UAS-GFP insertions that target GFP fluorescence to Twist expressing cells.  Embryos were collected and aged at 25°C to 5.5-7.5 hours after egg deposition (Stage 11).  GFP-negative cells were collected using FACS.


title: CGH_2005051163_Embryo2_cell2

source_name: Amplified single-cell (blastomere number 2) gDNA (XY)


title: lmd loss of function rep 3C

description: These are lmd-mutant embryos with Twi-GAL4 and UAS-GFP insertions that target GFP fluorescence to Twist expressing cells.  Embryos were collected and aged at 25°C to 6-8 hours after egg deposition (Stage 11).  GFP-positive cells were collected using FACS.


title: Notch gain of function rep 3A

description: These are embryos with Twi-GAL4 and UAS-GFP insertions that target GFP fluorescence to Twist expressing cells, as well as a UAS-Notch^{intra} insertion that yields constitutively activated Notch within these Twist expressing cells.  Embryos were collected and aged at 25°C to 6-8 hours after egg deposition (Stage 11).  GFP-positive cells were collected using FACS.


title: lmd loss of function rep 3A

tag: These are lmd-mutant embryos with Twi-GAL4 and UAS-GFP insertions that target GFP fluorescence to Twist expressing cells.  Embryos were collected and aged at 25°C to 6-8 hours after egg deposition (Stage 11).  GFP-positive cells were collected using FACS.


title: lmd loss of function rep 3B


title: Dl loss of function rep 3B

description: These are Dl-mutant embryos with Twi-GAL4 and UAS-GFP insertions that target GFP fluorescence to Twist expressing cells.  Embryos were collected and aged at 25°C to 6-8 hours after egg deposition (Stage 11).  GFP-positive cells were collected using FACS.


title: Dl loss of function rep 3C

tag: These are Dl-mutant embryos with Twi-GAL4 and UAS-GFP insertions that target GFP fluorescence to Twist expressing cells.  Embryos were collected and aged at 25°C to 6-8 hours after egg deposition (Stage 11).  GFP-positive cells were collected using FACS.


title: Notch gain of function rep 3C

tag: These are embryos with Twi-GAL4 and UAS-GFP insertions that target GFP fluorescence to Twist expressing cells, as well as a UAS-Notch^{intra} insertion that yields constitutively activated Notch within these Twist expressing cells.  Embryos were collected and aged at 25°C to 6-8 hours after egg deposition (Stage 11).  GFP-positive cells were collected using FACS.


title: Notch gain of function rep 3B


title: Dl loss of function rep 3A


title: wg loss of function rep 2B

description: These are wg-mutant embryos with Twi-GAL4 and UAS-GFP insertions that target GFP fluorescence to Twist expressing cells.  Embryos were collected and aged at 25°C to 5.5-7.5 hours after egg deposition (Stage 11).  GFP-positive cells were collected using FACS.


title: Tkv gain of function rep 2C

description: These are embryos with Twi-GAL4 and UAS-GFP insertions that target GFP fluorescence to Twist expressing cells, as well as a UAS-Tkv^{QD} insertion that yields constitutively activated Tkv within these Twist expressing cells.  Embryos were collected and aged at 25°C to 5.5-7.5 hours after egg deposition (Stage 11).  GFP-positive cells were collected using FACS.


title: Tkv gain of function rep 2A

tag: These are embryos with Twi-GAL4 and UAS-GFP insertions that target GFP fluorescence to Twist expressing cells, as well as a UAS-Tkv^{QD} insertion that yields constitutively activated Tkv within these Twist expressing cells.  Embryos were collected and aged at 25°C to 5.5-7.5 hours after egg deposition (Stage 11).  GFP-positive cells were collected using FACS.


title: Tkv gain of function rep 2B


title: wg loss of function rep 2C

tag: These are wg-mutant embryos with Twi-GAL4 and UAS-GFP insertions that target GFP fluorescence to Twist expressing cells.  Embryos were collected and aged at 25°C to 5.5-7.5 hours after egg deposition (Stage 11).  GFP-positive cells were collected using FACS.


title: Pnt gain of function rep 2C

description: These are embryos with Twi-GAL4 and UAS-GFP insertions that target GFP fluorescence to Twist expressing cells, as well as a UAS-Pnt^{P2VP16} insertion that yields constitutively activated Pnt within these Twist expressing cells.  Embryos were collected and aged at 25°C to 5.5-7.5 hours after egg deposition (Stage 11).  GFP-positive cells were collected using FACS.


title: Ras gain of function rep 2A

description: These are embryos with Twi-GAL4 and UAS-GFP insertions that target GFP fluorescence to Twist expressing cells, as well as a UAS-Ras1^{Act} insertion that yields constitutively activated Ras within these Twist expressing cells.  Embryos were collected and aged at 25°C to 5.5-7.5 hours after egg deposition (Stage 11).  GFP-positive cells were collected using FACS.


title: Arm+Ras gain of function rep 2C

description: These are embryos with Twi-GAL4 and UAS-GFP insertions that target GFP fluorescence to Twist expressing cells, as well as a UAS-Ras1^{Act} and UAS-Arm^{s10} insertions that yield constitutively activated Armadillo + Ras within these Twist expressing cells.  Embryos were collected and aged at 25°C to 5.5-7.5 hours after egg deposition (Stage 11).  GFP-positive cells were collected using FACS.


title: Ras gain of function rep 2B

tag: These are embryos with Twi-GAL4 and UAS-GFP insertions that target GFP fluorescence to Twist expressing cells, as well as a UAS-Ras1^{Act} insertion that yields constitutively activated Ras within these Twist expressing cells.  Embryos were collected and aged at 25°C to 5.5-7.5 hours after egg deposition (Stage 11).  GFP-positive cells were collected using FACS.


title: Ras gain of function rep 2C


title: Arm+Ras gain of function rep 2A

tag: These are embryos with Twi-GAL4 and UAS-GFP insertions that target GFP fluorescence to Twist expressing cells, as well as a UAS-Ras1^{Act} and UAS-Arm^{s10} insertions that yield constitutively activated Armadillo + Ras within these Twist expressing cells.  Embryos were collected and aged at 25°C to 5.5-7.5 hours after egg deposition (Stage 11).  GFP-positive cells were collected using FACS.


title: Arm+Ras gain of function rep 2B


title: Pnt gain of function rep 2B

tag: These are embryos with Twi-GAL4 and UAS-GFP insertions that target GFP fluorescence to Twist expressing cells, as well as a UAS-Pnt^{P2VP16} insertion that yields constitutively activated Pnt within these Twist expressing cells.  Embryos were collected and aged at 25°C to 5.5-7.5 hours after egg deposition (Stage 11).  GFP-positive cells were collected using FACS.


title: wg loss of function rep 2A


title: Arm gain of function rep 2C

description: These are embryos with Twi-GAL4 and UAS-GFP insertions that target GFP fluorescence to Twist expressing cells, as well as a UAS-Arm^{s10} insertion that yields constitutively activated Armadillo within these Twist expressing cells.  Embryos were collected and aged at 25°C to 5.5-7.5 hours after egg deposition (Stage 11).  GFP-positive cells were collected using FACS.


title: wild type GFP negative rep 1C


title: Arm gain of function rep 2B

tag: These are embryos with Twi-GAL4 and UAS-GFP insertions that target GFP fluorescence to Twist expressing cells, as well as a UAS-Arm^{s10} insertion that yields constitutively activated Armadillo within these Twist expressing cells.  Embryos were collected and aged at 25°C to 5.5-7.5 hours after egg deposition (Stage 11).  GFP-positive cells were collected using FACS.


title: Arm gain of function rep 2A