title: R80A_4h:Blank_4h - mAdb ID 56140

description:
mAdb experiment ID: 56140
Flp-In TREx 293 cells stably transfected with R80A-Vpr versus Flp-In TREx 293 cells stably transfected with blank vector harvested after 4 hrs of doxycycline treatment
Array Print: Hs-OperonV2-vB1.3p08-032904
cell_type: human embryonic kidney
cell_line: 293
Doxycycline treatment: 1 microgram/ml
treatment_time (hrs): 4
SAMPLE: R80A_4h:Blank_4h


title: HeLa_TP082505-36_HG-U133A_2_IMFGN0023

status: Public on Feb 05 2006

type: RNA

city: RTP

first: Todd

last: Page

state: NC

address: 6 Davis Dr

country: USA

institute: CIIT

description: TP082505-36_HG-U133A_2_IMFGN0023: HeLa cells were transfected with siRNA 48 hrs prior to heat shock.  At time=0 HeLa cells were placed in a 43C incubator for 1.5 hrs, at the indicated time points RNA was harvested and Affymetrix microarrays were run.

hyb_protocol: standard Affymetrix procedures

scan_protocol: standard Affymetrix procedures

data_processing: affylmGUI, The Walter and Eliza Hall Institute of Medical Research

label: biotin

molecule: total RNA

organism: Homo sapiens

source_name: Sample Type: Cells, Cell Line: HeLa, ATCC Number: CCL-2, Number of Cells Seeded: 2.20E+05, Cell Type: Cervical Carcinoma, Separation Type: Trypsin, Harvest Time Point: 24, Units of Harvest Time Point: hr

growth_protocol:
Growth Media: DMEM_10%FBS_P/S
Amount of Media: 5
Units of Media: ml
Growth Conditions: 37 C_5% CO2

extract_protocol: Extraction Method: Qiagen_RNeasy_Kit

treatment_protocol:
in vitro Treatment: siLuc (Sense 5'-rCrGUrArCrGrCrGrGrArAUrArCUUrCrGrCTT-3’ Antisense 5’-UrCrGrArArGUrAUUrCrCrGrCrGUrArCrGTT-3’), Treatment Repeat: 1, Units of Treatment Repeat: dose, Treatment Duration: 72, Units of Duration: hrs, Treatment Time: -48, Units of Treatment Time: hrs, Vehicle for Chemical: Lipofectamine`, Amount of Vehicle: 0.44, Route of Exposure: Transfection, Units of Amount of Vehicle: microliter, Dose or Concentration: 3.3, Units of Dose or Concentration: nM
Treatment_2: in vitro Treatment: Heat, 43ºC, Treatment Repeat: 1, Units of Treatment Repeat: exposure, Treatment Duration: 1.5, Units of Duration: hrs, Treatment Time: 0, Units of Treatment Time: hrs, Route of Exposure: Convection, Dose or Concentration: 43, Units of Dose or Concentration: degrees Celcius

tag: Array Type

tag: Ratio

tag: RNA Integrity Number

tag: Bioanalyzer Results

value: Excellent

tag: Sample Preservation


title: REC 133B

status: Public on Feb 08 2006

city: Bellinzona

first: Francesco

last: Bertoni

state: Ticino

address: Via Vela 6

department: Experimental Oncology

country: Switzerland

institute: Institute of Southern Switzerland

description: human B cell lymphoma from peripherial blood

hyb_protocol: standard Affymetrix procedures using Fluidics Station 400

scan_protocol: standard Affymetrix procedures using GeneChip Scanner 3000

data_processing: Affymetrix GeneChip GCOS 1.1

source_name: REC

label_protocol: standard Affymetrix protocol starting from 10 µg of total RNA.

extract_protocol: RNA was extracted from cell lines with the Trizol method (Trizol, Invitrogen Life Technologies, San Diego, CA, USA) with an additional RNA clean-up step using the RNAeasy Purification kit.

tag: human B cell lymphoma established cell line


title: R80A_2h:Blank_2h - mAdb ID 56139

status: Public on Feb 10 2006

city: Bethesda

first: Naoto

last: Yoshizuka

state: MD

institute: NCI

description:
mAdb experiment ID: 56139
Flp-In TREx 293 cells stably transfected with R80A-Vpr versus Flp-In TREx 293 cells stably transfected with blank vector harvested after 2 hrs of doxycycline treatment
Array Print: Hs-OperonV2-vB1.3p08-032904
cell_type: human embryonic kidney
cell_line: 293
Doxycycline treatment: 1 microgram/ml
treatment_time (hrs): 2
SAMPLE: R80A_2h:Blank_2h


title: R80A_16h:Blank_16h - mAdb ID 55200

description:
mAdb experiment ID: 55200
Flp-In TREx 293 cells stably transfected with R80A-Vpr versus Flp-In TREx 293 cells stably transfected with blank vector harvested after 16 hrs of doxycycline treatment
Array Print: Hs-OperonV2-vB1.2p26-012004
cell_type: human embryonic kidney
cell_line: 293
Doxycycline treatment: 1 microgram/ml
treatment_time (hrs): 16
SAMPLE: R80A_16h:Blank_16h


title: F72A/R73A_0h:Blank_0h - mAdb ID 56122

description:
mAdb experiment ID: 56122
Flp-In TREx 293 cells stably transfected with F72A/R73A-Vpr versus Flp-In TREx 293 cells stably transfected with blank vector harvested after 0 hr of doxycycline treatment
Array Print: Hs-OperonV2-vB1.3p08-032904
cell_type: human embryonic kidney
cell_line: 293
Doxycycline treatment: none
treatment_time (hrs): 0
SAMPLE: F72A/R73A_0h:Blank_0h


title: R80A_24h:Blank_24h - mAdb ID 55201

description:
mAdb experiment ID: 55201
Flp-In TREx 293 cells stably transfected with R80A-Vpr versus Flp-In TREx 293 cells stably transfected with blank vector harvested after 24 hrs of doxycycline treatment
Array Print: Hs-OperonV2-vB1.2p26-012004
cell_type: human embryonic kidney
cell_line: 293
Doxycycline treatment: 1 microgram/ml
treatment_time (hrs): 24
SAMPLE: R80A_24h:Blank_24h


title: HeLa_TP082505-33_HG-U133A_2_IMFGN0023

description: TP082505-33_HG-U133A_2_IMFGN0023: HeLa cells were transfected with siRNA 48 hrs prior to heat shock.  At time=0 HeLa cells were placed in a 43C incubator for 1.5 hrs, at the indicated time points RNA was harvested and Affymetrix microarrays were run.

source_name: Sample Type: Cells, Cell Line: HeLa, ATCC Number: CCL-2, Number of Cells Seeded: 2.20E+05, Cell Type: Cervical Carcinoma, Separation Type: Trypsin, Harvest Time Point: 12, Units of Harvest Time Point: hr

treatment_protocol:
in vitro Treatment: siLuc (Sense 5'-rCrGUrArCrGrCrGrGrArAUrArCUUrCrGrCTT-3’ Antisense 5’-UrCrGrArArGUrAUUrCrCrGrCrGUrArCrGTT-3’), Treatment Repeat: 1, Units of Treatment Repeat: dose, Treatment Duration: 60, Units of Duration: hrs, Treatment Time: -48, Units of Treatment Time: hrs, Vehicle for Chemical: Lipofectamine`, Amount of Vehicle: 0.44, Route of Exposure: Transfection, Units of Amount of Vehicle: microliter, Dose or Concentration: 3.3, Units of Dose or Concentration: nM
Treatment_2: in vitro Treatment: Heat, 43ºC, Treatment Repeat: 1, Units of Treatment Repeat: exposure, Treatment Duration: 1.5, Units of Duration: hrs, Treatment Time: 0, Units of Treatment Time: hrs, Route of Exposure: Convection, Dose or Concentration: 43, Units of Dose or Concentration: degrees Celcius


title: HeLa_TP082505-32_HG-U133A_2_IMFGN0023

description: TP082505-32_HG-U133A_2_IMFGN0023: HeLa cells were transfected with siRNA 48 hrs prior to heat shock.  At time=0 HeLa cells were placed in a 43C incubator for 1.5 hrs, at the indicated time points RNA was harvested and Affymetrix microarrays were run.


title: HeLa_TP082505-34_HG-U133A_2_IMFGN0023

description: TP082505-34_HG-U133A_2_IMFGN0023: HeLa cells were transfected with siRNA 48 hrs prior to heat shock.  At time=0 HeLa cells were placed in a 43C incubator for 1.5 hrs, at the indicated time points RNA was harvested and Affymetrix microarrays were run.


title: R80A_24h:Blank_24h - mAdb ID 56149

description:
mAdb experiment ID: 56149
Flp-In TREx 293 cells stably transfected with R80A-Vpr versus Flp-In TREx 293 cells stably transfected with blank vector harvested after 24 hrs of doxycycline treatment
Array Print: Hs-OperonV2-vB1.3p08-032904
cell_type: human embryonic kidney
cell_line: 293
Doxycycline treatment: 1 microgram/ml
treatment_time (hrs): 24
SAMPLE: R80A_24h:Blank_24h


title: F72A/R73A_16h:Blank_16h - mAdb ID 56131

description:
mAdb experiment ID: 56131
Flp-In TREx 293 cells stably transfected with F72A/R73A-Vpr versus Flp-In TREx 293 cells stably transfected with blank vector harvested after 16 hrs of doxycycline treatment
Array Print: Hs-OperonV2-vB1.3p08-032904
cell_type: human embryonic kidney
cell_line: 293
Doxycycline treatment: 1 microgram/ml
treatment_time (hrs): 16
SAMPLE: F72A/R73A_16h:Blank_16h


title: HeLa_TP082505_23_HG-U133A_2_IMFGN0023

description: TP082505_23_HG-U133A_2_IMFGN0023: HeLa cells were transfected with siRNA 48 hrs prior to heat shock.  At time=0 HeLa cells were placed in a 43C incubator for 1.5 hrs, at the indicated time points RNA was harvested and Affymetrix microarrays were run.

source_name: Sample Type: Cells, Cell Line: HeLa, ATCC Number: CCL2, Number of Cells Seeded: 2.20E+05, Cell Type: Cervical Carcinoma, Separation Type: Trypsin, Harvest Time Point: 0.5, Units of Harvest Time Point: hr

treatment_protocol:
in vitro Treatment: siLuc (Sense 5'-rCrGUrArCrGrCrGrGrArAUrArCUUrCrGrCTT-3’ Antisense 5’-UrCrGrArArGUrAUUrCrCrGrCrGUrArCrGTT-3’), Treatment Repeat: 1, Units of Treatment Repeat: dose, Treatment Duration: 48.5, Units of Duration: hrs, Treatment Time: -48, Units of Treatment Time: hrs, Vehicle for Chemical: Lipofectamine 2000, Amount of Vehicle: 0.44, Route of Exposure: Transfection, Units of Amount of Vehicle: microliter, Dose or Concentration: 3.3, Units of Dose or Concentration: nM
Treatment_2: in vitro Treatment: Heat, 43ºC, Treatment Repeat: 1, Units of Treatment Repeat: exposure, Treatment Duration: 0.5, Units of Duration: hrs, Treatment Time: 0, Units of Treatment Time: hrs, Route of Exposure: Convection, Dose or Concentration: 43, Units of Dose or Concentration: degrees Celcius


title: HeLa_TP082505_24_HG-U133A_2_IMFGN0023

description: TP082505_24_HG-U133A_2_IMFGN0023: HeLa cells were transfected with siRNA 48 hrs prior to heat shock.  At time=0 HeLa cells were placed in a 43C incubator for 1.5 hrs, at the indicated time points RNA was harvested and Affymetrix microarrays were run.


title: HeLa_TP082505-35_HG-U133A_2_IMFGN0023

description: TP082505-35_HG-U133A_2_IMFGN0023: HeLa cells were transfected with siRNA 48 hrs prior to heat shock.  At time=0 HeLa cells were placed in a 43C incubator for 1.5 hrs, at the indicated time points RNA was harvested and Affymetrix microarrays were run.


title: R80A_12h:Blank_12h - mAdb ID 55199

description:
mAdb experiment ID: 55199
Flp-In TREx 293 cells stably transfected with R80A-Vpr versus Flp-In TREx 293 cells stably transfected with blank vector harvested after 12 hrs of doxycycline treatment
Array Print: Hs-OperonV2-vB1.2p26-012004
cell_type: human embryonic kidney
cell_line: 293
Doxycycline treatment: 1 microgram/ml
treatment_time (hrs): 12
SAMPLE: R80A_12h:Blank_12h


title: HeLa_TP082505-26_HG-U133A_2_IMFGN0023

description: TP082505-26_HG-U133A_2_IMFGN0023: HeLa cells were transfected with siRNA 48 hrs prior to heat shock.  At time=0 HeLa cells were placed in a 43C incubator for 1.5 hrs, at the indicated time points RNA was harvested and Affymetrix microarrays were run.

source_name: Sample Type: Cells, Cell Line: HeLa, ATCC Number: CCL-2, Number of Cells Seeded: 2.20E+05, Cell Type: Cervical Carcinoma, Separation Type: Trypsin, Harvest Time Point: 2, Units of Harvest Time Point: hr

treatment_protocol:
in vitro Treatment: siLuc (Sense 5'-rCrGUrArCrGrCrGrGrArAUrArCUUrCrGrCTT-3’ Antisense 5’-UrCrGrArArGUrAUUrCrCrGrCrGUrArCrGTT-3’), Treatment Repeat: 1, Units of Treatment Repeat: dose, Treatment Duration: 50, Units of Duration: hrs, Treatment Time: -48, Units of Treatment Time: hrs, Vehicle for Chemical: Lipofectamine`, Amount of Vehicle: 0.44, Route of Exposure: Transfection, Units of Amount of Vehicle: microliter, Dose or Concentration: 3.3, Units of Dose or Concentration: nM
Treatment_2: in vitro Treatment: Heat, 43ºC, Treatment Repeat: 1, Units of Treatment Repeat: exposure, Treatment Duration: 1.5, Units of Duration: hrs, Treatment Time: 0, Units of Treatment Time: hrs, Route of Exposure: Convection, Dose or Concentration: 43, Units of Dose or Concentration: degrees Celcius


title: R80A_4h:Blank_4h - mAdb ID 55196

description:
mAdb experiment ID: 55196
Flp-In TREx 293 cells stably transfected with R80A-Vpr versus Flp-In TREx 293 cells stably transfected with blank vector harvested after 4 hrs of doxycycline treatment
Array Print: Hs-OperonV2-vB1.2p26-012004
cell_type: human embryonic kidney
cell_line: 293
Doxycycline treatment: 1 microgram/ml
treatment_time (hrs): 4
SAMPLE: R80A_4h:Blank_4h


title: R80A_2h:Blank_2h - mAdb ID 55195

description:
mAdb experiment ID: 55195
Flp-In TREx 293 cells stably transfected with R80A-Vpr versus Flp-In TREx 293 cells stably transfected with blank vector harvested after 2 hrs of doxycycline treatment
Array Print: Hs-OperonV2-vB1.2p26-012004
cell_type: human embryonic kidney
cell_line: 293
Doxycycline treatment: 1 microgram/ml
treatment_time (hrs): 2
SAMPLE: R80A_2h:Blank_2h


title: R80A_6h:Blank_6h - mAdb ID 55197

description:
mAdb experiment ID: 55197
Flp-In TREx 293 cells stably transfected with R80A-Vpr versus Flp-In TREx 293 cells stably transfected with blank vector harvested after 6 hrs of doxycycline treatment
Array Print: Hs-OperonV2-vB1.2p26-012004
cell_type: human embryonic kidney
cell_line: 293
Doxycycline treatment: 1 microgram/ml
treatment_time (hrs): 6
SAMPLE: R80A_6h:Blank_6h


title: R80A_0h:Blank_0h - mAdb ID 56137

description:
mAdb experiment ID: 56137
Flp-In TREx 293 cells stably transfected with R80A-Vpr versus Flp-In TREx 293 cells stably transfected with blank vector harvested after 0 hr of doxycycline treatment
Array Print: Hs-OperonV2-vB1.3p08-032904
cell_type: human embryonic kidney
cell_line: 293
Doxycycline treatment: none
treatment_time (hrs): 0
SAMPLE: R80A_0h:Blank_0h


title: R80A_8h:Blank_8h - mAdb ID 55198

description:
mAdb experiment ID: 55198
Flp-In TREx 293 cells stably transfected with R80A-Vpr versus Flp-In TREx 293 cells stably transfected with blank vector harvested after 8 hrs of doxycycline treatment
Array Print: Hs-OperonV2-vB1.2p26-012004
cell_type: human embryonic kidney
cell_line: 293
Doxycycline treatment: 1 microgram/ml
treatment_time (hrs): 8
SAMPLE: R80A_8h:Blank_8h


title: 18KB_427_18_U_i_h

status: Public on Dec 31 2005

city: Lindau

first: Stefan

middle: Michael

last: Bauersachs

state: Zurich

address: Eschikon 27 EHB 23.1

department: Department for Farm Animals

institute: University of Zurich

description: The array hybridization with ten cDNA probes corresponding to the ten animals (Day 18 pregnant animals n=4, controls n=6) was processed simultaneously, each with two arrays (LAFUGA Cattle subtr lib 18KB v1.0 and LAFUGA Cattle subtr lib 18KO v1.0).

hyb_protocol: Hybridization was done as follows: Pre-hybridization was done for up to six arrays together in one 15 cm glass hybridization bottle: 3 x 10 min 10 ml 1 x PBS / 10 % SDS at 65 °C; 2 x 10 min 10 ml 0.1 x PBS / 1 % SDS at 85 °C; 3 x 10 min 10 ml 1 x PBS / 10 % SDS at 65 °C. Hybridization probes were denatured for 15 min at 96 °C immediately before adding to the hybridization solution. Hybridization was done in plastic vials (Poly-Q vials, 18 ml, Beckman Coulter, München, Germany) in 2 ml 1 x PBS, pH 7.5 / 10 % SDS for 45 hrs at 65 °C. After hybridization, arrays were put together in one 15 cm glass hybridization bottle and washed as follows: 3 x 5 min 10 ml 1 x PBS / 10 % SDS at 65 °C; 3 x 10 min 10 ml 1 x PBS / 10 % SDS at 65 °C; 3 x 10 min 10 ml 0.1 x PBS / 1 % SDS at 65 °C and finally 2 x 5 min 10 ml 1 x PBS / 1 % SDS / 2 mmol/l EDTA at room temperature. Filters were dried by baking at 80 °C for 20 min and exposed to an imaging plate BAS-SR (Fuji Photo Film Co., Ltd.).

scan_protocol: Imaging plates were scanned with a phosphor imager (Storm 860, Amersham Biosciences).

data_processing: Array evaluation: AIDA Image Analyzer software (Version 3.52, Raytest, Straubenhardt, Germany); background subtraction: lowest grid dot function; raw data were exported to Microsoft Excel; Normalization: (integral-background) over median of (integral-backgrounds) of 70 reference cDNAs, which showed the lowest CV over all hybridizations; normalized values were then multiplied by 21 (values of detectable signals >=1.0)

organism: Bos taurus

source_name: Uterus, endometrium, ipsilateral, caudal uterine horn, Day 18 of estrous cycle

label_protocol: Double-stranded cDNA was synthesized starting from 50 µg total RNA using Superscript III (Invitrogen) for first strand synthesis and cDNA primer 1 (AACTGCGGCCGCGTACAGC(T20)VN). Synthesis of the second strand was performed with RNase H, E. coli DNA ligase, and E. coli DNA polymerase I (Invitrogen) according to the instructions of the manufacturer. 33P-labeled cDNA probes were generated from ds cDNA corresponding to appr. 10 µg of total RNA. cDNA was heat-denatured for 10 min at 96 °C and then chilled on ice. High Prime reaction mixture (Roche Molecular Biochemicals, Mannheim, Germany), dNTP mixture (dCTP final concentration 10 pmol/µl, dATP, dGTP, and dTTP final concentration each 100 pmol/µl) and 90 µCi [33P]-dCTP were added to a final volume of 20 µl. Reactions were incubated for 4 hrs at 37 °C, 20 min at 65 °C and subsequently purified with ProbeQuant G50 Micro Columns (Amersham Biosciences, Freiburg, Germany) to remove unincorporated nucleotides and to estimate labeling efficiency.

extract_protocol: Total RNA from endometrial tissue samples was isolated using Trizol reagent (Invitrogen, Karlsruhe, Germany) according to the manufacturer's recommendations. The quantity and quality of total RNA was determined by spectrometry and agarose gel electrophoresis.

treatment_protocol: Cyclic heifers were cycle synchronized by injecting intramuscularly a single dose of 500 µg Cloprostenol (Estrumate®; Essex Tierarznei, Munich, Germany) at dioestrus. Animals were observed for sexual behavior (i.e., toleration, sweating, vaginal mucus) to determine standing heat, which occurred around 60 h after Estrumate injection. In addition, animals were checked with ultrasound-guided follicle monitoring. Blood samples were taken at day 20, day 0 of the estrous cycle and just before slaughtering to determine serum progesterone (P4) levels. Control animals were inseminated with the supernatant of cryo-conserved sperm (ejaculate + diluter 1:10) after centrifugation at estrus and slaughtered at Day 18 of the estrous cycle. No conceptus was detected. Sperm was derived from the same bull. The uterus was prepared and opened longitudinally. The uterus was divided into seven sections: corpus plus caudal, middle and cranial parts of the ipsilateral and the contralateral uterine horns (for details see Bauersachs S et al. 2005). Samples were carefully cut out from the lamina propria of the intercaruncular endometrium with a scalpel and immediately transferred into vials containing 4 ml RNAlater (Ambion, Huntingdon, Cambridgeshire, UK) and incubated on wet ice overnight. Samples were stored at -20°C until further processing.

tag: Simmental heifer (Deutsches Fleckvieh), Age

value: 22 month, Animal ID:427


title: R80A_0h:Blank_0h - mAdb ID 55193

description:
mAdb experiment ID: 55193
Flp-In TREx 293 cells stably transfected with R80A-Vpr versus Flp-In TREx 293 cells stably transfected with blank vector harvested after 0 hr of doxycycline treatment
Array Print: Hs-OperonV2-vB1.2p26-012004
cell_type: human embryonic kidney
cell_line: 293
Doxycycline treatment: none
treatment_time (hrs): 0
SAMPLE: R80A_0h:Blank_0h


title: R80A_1h:Blank_1h - mAdb ID 55194

description:
mAdb experiment ID: 55194
Flp-In TREx 293 cells stably transfected with R80A-Vpr versus Flp-In TREx 293 cells stably transfected with blank vector harvested after 1 hr of doxycycline treatment
Array Print: Hs-OperonV2-vB1.2p26-012004
cell_type: human embryonic kidney
cell_line: 293
Doxycycline treatment: 1 microgram/ml
treatment_time (hrs): 1
SAMPLE: R80A_1h:Blank_1h


title: 18KB_449_18_U_i_h

value: 18 month, Animal ID:449


title: 18KB_404_18_U_i_h

value: 20 month, Animal ID:404


title: 18KB_330_18_U_i_h

value: 20 month, Animal ID:330


title: 18KB_440_18_U_i_h

value: 22 month, Animal ID:440


title: 18KB_434_18_U_i_h

value: 19 month, Animal ID:434


title: 18KO_449_18_U_i_h

data_processing: Array evaluation: AIDA Image Analyzer software (Version 3.52, Raytest, Straubenhardt, Germany); background subtraction: weighted image regions function; raw data were exported to Microsoft Excel; Normalization: (integral-background) over median of (integral-backgrounds) of 45 reference cDNAs, which showed the lowest CV over all hybridizations; normalized values were then multiplied by 21 (values of detectable signals >=1.0)


title: 18KO_404_18_U_i_h


title: 18KB_316_18_U_i_h

source_name: Uterus, endometrium, ipsilateral, caudal uterine horn, Day 18 of pregnancy

treatment_protocol: Cyclic heifers were cycle synchronized by injecting intramuscularly a single dose of 500 µg Cloprostenol (Estrumate®; Essex Tierarznei, Munich, Germany) at dioestrus. Animals were observed for sexual behavior (i.e., toleration, sweating, vaginal mucus) to determine standing heat, which occurred around 60 h after Estrumate injection. In addition, animals were checked with ultrasound-guided follicle monitoring. Blood samples were taken at day 20, day 0 of the estrous cycle and just before slaughtering to determine serum progesterone (P4) levels. Animals were inseminated with cryo-conserved sperm (ejaculate + diluter 1:10) at estrus and  slaughtered at Day 18 of gestation and intact concepti were detected in the ipsilateral uterine horns of the animals used for gene expression analysis. Sperm was derived from the same bull. The uterus was prepared and opened longitudinally. In pregnant animals, the conceptus was localized and carefully removed. The uterus was divided into seven sections: corpus plus caudal, middle and cranial parts of the ipsilateral and the contralateral uterine horns (for details see Bauersachs S et al. 2005). Samples were carefully cut out from the lamina propria of the intercaruncular endometrium with a scalpel and immediately transferred into vials containing 4 ml RNAlater (Ambion, Huntingdon, Cambridgeshire, UK) and incubated on wet ice overnight. Samples were stored at -20°C until further processing.

value: 20 month, Animal ID:316


title: 18KO_434_18_U_i_h


title: 18KB_447_18_U_i_h

value: 19 month, Animal ID:447


title: 18KO_427_18_U_i_h


title: 18KO_316_18_U_i_h

description: The hybridization of the sample 316_18_U_i_h with the array LAFUGA Cattle subtr lib 18KO v1.0, which was done nsimultaneously with the other 9 samples could not be analyzed due to blurred spots on the array. The hybridization with 316_18_U_i_h was repeated under the same conditions simultaneously with two of the other samples. After normalization, data of the sample 316_18_U_i_h were added to the other dataset based on the ratios to the other two samples.

treatment_protocol: Cyclic heifers were cycle synchronized by injecting intramuscularly a single dose of 500 µg Cloprostenol (Estrumate®; Essex Tierarznei, Munich, Germany) at dioestrus. Animals were observed for sexual behavior (i.e., toleration, sweating, vaginal mucus) to determine standing heat, which occurred around 60 h after Estrumate injection. In addition, animals were checked with ultrasound-guided follicle monitoring. Blood samples were taken at day 20, day 0 of the estrous cycle and just before slaughtering to determine serum progesterone (P4) levels. Animals were inseminated with cryo-conserved sperm (ejaculate + diluter 1:10) at estrus and slaughtered at Day 18 of gestation and intact concepti were detected in the ipsilateral uterine horns of the animals used for gene expression analysis. Sperm was derived from the same bull. The uterus was prepared and opened longitudinally. In pregnant animals, the conceptus was localized and carefully removed. The uterus was divided into seven sections: corpus plus caudal, middle and cranial parts of the ipsilateral and the contralateral uterine horns (for details see Bauersachs S et al. 2005). Samples were carefully cut out from the lamina propria of the intercaruncular endometrium with a scalpel and immediately transferred into vials containing 4 ml RNAlater (Ambion, Huntingdon, Cambridgeshire, UK) and incubated on wet ice overnight. Samples were stored at -20°C until further processing.


title: 18KB_426_18_U_i_h

value: 17 month, Animal ID:426


title: 18KO_426_18_U_i_h


title: 18KO_447_18_U_i_h


title: SON-C-D_6r

status: Public on Dec 02 2005

city: Bristol

first: DAVID

last: MURPHY

state: AVON

address: Whitson Street

department: Medicine and Dentistry

country: United Kingdom

institute: University of Bristol

description: SON-C-D Clone array - 3d dehydrated

data_processing: ImageQuant software (Amersham Biosciences, Little Chalfont, UK)

label: [Alpha-33P] CTP radiolabel

organism: Rattus norvegicus

source_name: Supraoptic nucleus_3d dehydrated

label_protocol: Radiolabelled target was generated from 3-10mg of total SON RNA (from either C or D rats) template. [±-33P] CTP was incorporated into cDNA using Superscript II reverse transcriptase (Invitrogen, Paisley, Scotland) and10-20-mer poly(dT) primer (Invitrogen, Paisley, Scotland).

extract_protocol: RNA isolation - Animals were killed by cervical dislocation and total cellular RNA was isolated from microdissected SON using TRIzol (Invitrogen, Paisley, Scotland).

tag: Strain

value: Sprague Dawley

tag: Gender

value: Male

tag: Age

value: 10-12 weeks

tag: Tissue

value: Supraoptic nucleus


title: 18KO_440_18_U_i_h


title: 18KO_330_18_U_i_h


title: SON-C-D_5r

description: SON-C-D Clone array - 3d dehydrated


title: SON-C-D_19r

description: SON-C-D Clone array - 3d dehydrated


title: SON-C-D_18r

description: SON-C-D Clone array - 3d dehydrated

source_name: Supraoptic nucleus_3d Dehydrated


title: 18KO_448_18_U_i_h

value: 20 month, Animal ID:448


title: SON-C-D_4r

description: SON-C-D Clone array - control

source_name: Supraoptic nucleus_control


title: SON-C-D_17r

description: SON-C-D Clone array - control

source_name: Supraoptic nucleus


title: SON-C-D_16r

description: SON-C-D Clone array - control


title: SON-C-D_3r

description: SON-C-D Clone array - control


title: Heat-shock(Temp43, 60min)-3, (low power scan)

status: Public on Sep 21 2005

city: Tsukuba

first: Rena

last: Matsumoto

state: Ibaraki

address: 1-1-1 Higashi

country: Japan

institute: National Institute of Advanced Industrial Science and Technology

description: The ratio of intensity Cy5/Cy3 was calculated and normalized with negative control spots. In order to rescue data that had a higher intensity than the scanning range, the array were scanned twice with different laser power. This data is result from low power scanning.

hyb_protocol: Synthesized cDNA were hybridized to Kuhara DNA chip (DNA Chip Research, Yokohama, Kanagawa, Japan) at 65 degrees C for 48 h.

data_processing: GeneSpring (Silicon Genetics)

molecule: polyA RNA

organism: Saccharomyces cerevisiae

source_name: Heat-shock(Temp43, 60min), Cy5

label_protocol: Cyscribe first-strand, cDNA labeling kit (Amersham Biosciences)

growth_protocol: JN54 (wild-type) cells were incubated in YPD medium at 30 degrees C to a logarithmic phase (OD660=1), followed by treatment with mild heat-shock at 43 degrees C for 60 min in pre-warmed (43 degrees C) 100ml of YPD medium using 500 ml Erlenmeyer flask.

extract_protocol: Hot-phenol and oligotex dt mRNA purification kit (TaKaRa)

tag: strain JN54 (wild-type)

source_name: Control (temp30, 60min), Cy3

growth_protocol: JN54 (wild-type) cells were incubated in YPD medium at 30 degrees C to a logarithmic phase (OD660=1), followed by incubated at 30 degrees C for 60 min in 100ml of YPD medium using 500 ml Erlenmeyer flask.


title: Heat-shock(Temp43, 60min)-2, (low power scan)


title: Raji 3

status: Public on Mar 31 2005

city: Chapel Hill

first: Scott

middle: M

last: Hammond

department: Dept Cell and Developmental Biology

institute: University of North Carolina

description:
5 ug total RNA,  was labeled with Cy3 and hybridized to the microRNA microarray.  Cy3 median intensities were background subtracted, filtered to remove genes that did not have cy3> 2x background.  Log cy3 values were median center normalized and are represented by the ref_value.
Keywords = microRNA, miRNA

source_name: Labeled microRNA


title: Raji 4


title: Manca 4


title: HG-1125 4


title: Heat-shock(Temp43, 30min)-3, (low power scan)

source_name: Heat-shock(Temp43, 30min), Cy5

growth_protocol: JN54 (wild-type) cells were incubated in YPD medium at 30 degrees C to a logarithmic phase (OD660=1), followed by treatment with mild heat-shock at 43 degrees C for 30 min in pre-warmed (43 degrees C) 100ml of YPD medium using 500 ml Erlenmeyer flask.

source_name: Control (temp30, 30min), Cy3

growth_protocol: JN54 (wild-type) cells were incubated in YPD medium at 30 degrees C to a logarithmic phase (OD660=1), followed by incubated at 30 degrees C for 30 min in 100ml of YPD medium using 500 ml Erlenmeyer flask.


title: Heat-shock(Temp43, 60min)-2, (high power scan)

description: The ratio of intensity Cy5/Cy3 was calculated and normalized with negative control spots. In order to rescue data that had a higher intensity than the scanning range, the array were scanned twice with different laser power. This data is result from high power scanning.


title: Heat-shock(Temp43, 30min)-2, (high power scan)


title: Heat-shock(Temp43, 30min)-2, (low power scan)


title: Heat-shock(Temp43, 30min)-3, (high power scan)


title: Heat-shock(Temp43, 60min)-3, (high power scan)


title: Heat-shock(Temp43, 30min)-1, (low power scan)

description: The ratio of intensity Cy5/Cy3 was calculated and normalized with negative control spots. In order to rescue data that had a higher intensity than the scanning range, the array were scanned twice with different laser power. This data is result from low power scanning

source_name: Heat-shock(Temp43, 30min)-1, Cy5


title: OCI-Ly7 4


title: Heat-shock(Temp43, 30min)-1, (high power scan)


title: Heat-shock(Temp43, 60min)-1, (high power scan)


title: Heat-shock(Temp43, 60min)-1, (low power scan)


title: Gene expression profiling of C2C12 MyoD-silenced clone at 1 day of myogenic differentiation (A)

status: Public on Jul 28 2005

city: Padova

first: Gerolamo

last: Lanfranchi

address: Via U. Bassi, 58/B

department: CRIBI - Biotechnology Center and Biology Department

country: Italy

institute: University of Padova

description: RNA purified from C2C12 MyoD(-)cl.A8 at one myogenic differentiation time-points was compared with RNA from C2C12 control cells at the corresponding differentiation stages. These samples were labeled and hybridized to muscle specific microarrays produced by our group (Human Array 1.0).

hyb_protocol: Microarray hybridisation was carried out in a dual slide chamber (HybChamber, Gene Machines, San Carlos, CA, USA) humidified with 100 µl of 3 x SSC. Labeled cDNA was dissolved in 40 µl of hybridisation buffer, denatured at 90°C for 2 min in a thermal cycler and applied directly to the slides. Microarrays were covered with 22 x 40 mm cover slip and hybridized overnight at 65°C by immersion in a high precision water bath (W28, Grant, Cambridge, UK). Post-hybridization washing was performed according to the MICROMAX TSA Detection kit (PerkinElmer).

scan_protocol: Digital images were generated in a GSI Lumonics LITE dual confocal laser scanner (ScanArray Microarray Analysis Software) and processed with QuantArray Analysis Software (GSI Lumonics, Ottawa, Canada).

data_processing: The gene expression data analysis tool SNOMAD (http://pevsnerlab.kennedykrieger.org/snomadinput.html) was used for microarray data normalization and standardization. Log2 transformation was performed for each normalized expression value.

organism: Mus musculus

source_name: C2C12 control cells (Reference)

label_protocol: Total RNA was retro-transcribed and labeled using a MICROMAX TSA Labeling Kit (PerkinElmer). Two ug of total RNA were used in each reaction but only half of the labeled cDNA was actually hybridized to the microarrays.

extract_protocol: Total RNA was purified from C2C12 control cells following the standard Trizol protocol. The RNA 6000 LabChip kit (Agilent Technologies) was used for RNA quantification and quality control in conjunction with an Agilent Bioanalyzer 2001.

tag: - C2C12 cell line

tag: - Species

value: mouse, C3H; Tissue:muscle

tag: - Morphology

value: Myoblast

tag: - Depositor

value: Obtained from ATCC, USA

tag: - Properties

value: differentiation; muscle proteins express; myogenesis

source_name: C2C12 MyoD-silenced cells (Test)

extract_protocol: Total RNA was purified from C2C12 MyoD(-) cells (MyoD(-)cl.A8) after one day of myogenic differentiation following the standard Trizol protocol. The RNA 6000 LabChip kit (Agilent Technologies) was used for RNA quantification and quality control in conjunction with an Agilent Bioanalyzer 2001.

tag: - C2C12 MyoD-silenced cells

value: the mouse MyoD transcript was cloned in pcDNA 3.0 mammalian expression vector in antisense orientation and the construct was transfected in C2C12 cells. The cells were selected for ten days and the G-418 resistant clones were tested for MyoD silencing.

tag: - MyoD knock-down clone

tag: - Growing medium

value: DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)

tag: - Differentiating medium

value: DMEM + 2mM Glutamine + 2% Horse Serum (HS)


title: Jurkat 4


title: OCI-Ly8 4


title: Manca 3


title: Namalwa 4


title: HG-1125 3


title: Karpas-1718 4


title: OCI-Ly4 4


title: Jurkat 3


title: OCI-Ly7 3


title: OCI-Ly8 3


title: Karpas-1718 3


title: OCI-Ly8 2


title: HG-1125 2


title: OCI-Ly4 3


title: normal B cell 4


title: Manca 1


title: OCI-Ly4 2


title: Jurkat 2


title: HG-1125 1


title: OCI-Ly7 2


title: normal B cell 3


title: Manca 2


title: OCI-Ly7 1


title: OCI-Ly8 1


title: Namalwa 3


title: Raji 2


title: Raji 1


title: Jurkat 1


title: Karpas-1718 2


title: Karpas-1718 1


title: Normal B cell 1


title: Namalwa 1


title: Namalwa 2


title: Normal B cell 2


title: OCI-Ly4 1


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Clo:18:4152TUM)

status: Public on Mar 01 2005

city: VITRY / SEINE

first: Cecile

last: Michel

address: 13, quai Jules Guesde

department: DSE

country: France

institute: Sanofi-Aventis

description:
Liver sections from F344 rats treated with Clofibric Acid (CA)
Keywords = Clofibric-Acid, DEN

data_processing: The scanned image was quantitatively analyzed using MAS5.0; one chip from 5days-control group was used to normalize all GeneChips


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Clo:18:4152TUG)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Clo:18:4156TUM)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Clo:18:4152TUD)


title: 18KB_448_18_U_i_h


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Clo:18:4152)


title: Gene expression data from liver of rats treated with 5000 ppm CA for 12 month rev(DEN-Clo::4113bis)

source_name: DEN-Clo:


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Clo:18:4158)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN-Clo:9m:4105B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for 12rev month (DEN-Clo::4112)


title: Gene expression data from liver of rats treated with 5000 ppm CA for 12rev month (DEN-Clo::4111TU)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN-Clo:9m:4103TUMB)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN-Clo:5j:4087B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN-Clo:9m:4102B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Clo:18:4156)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Clo:18:4153)


title: Gene expression data from liver of rats treated with 5000 ppm CA for 12rev month (DEN-Clo::4111TUMI)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN-Clo:9m:4104B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Clo:18:4155)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN-Clo:9m:4105TUMB)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN-Clo:5j:4086)


title: Gene expression data from liver of rats treated with 5000 ppm CA for 12rev month (DEN-Clo::4116)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN-Clo:9m:4104TUM)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN-Clo:5j:4088B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for 12 rev month (DEN-Clo::4111)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN-Clo:5j:4089)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN-Clo:9m:4104PERI)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN-Clo:9m:4115)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN-Clo:3m:4098)


title: Gene expression data from liver of rats treated with 5000 ppm CA for 12 rev month (DEN-Clo::4114)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN-Clo:9m:4103B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Clo:18:4157)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN-Clo:5j:4085)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN-Clo:3m:4096B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN-Clo:3m:4095B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN-Clo:1m:4094B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN-Clo:3m:4097B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN-Clo:3m:4099B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN-Clo:1m:4093B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN-Clo:1m:4090)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN-Clo:12m:4110)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN:9m:4069)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN:9m:4070Bbis)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN-Clo:12m:4106)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN:9m:4071)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN-Clo:1m:4091B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN:12rev:4080)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN-Clo:12m:4108)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN-Clo:12m:4107)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN-Clo:12m:4109TUM)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN-Clo:12m:4109)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN:12rev:4083)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN-Clo:12m:4107TUM)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN-Clo:12m:4106TUM)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN:12rev:4078)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN-Clo:12m:4109PERI)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN:12rev:4084)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN:9m:4072)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN:12rev:4081)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN:-8:4052B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN:12rev:4082bis)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN:9m:4068)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN:-8:4051B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN:-8:4050B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN:-8:4048B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN:-8:4049)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN:5j:4056B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN:5j:4057B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN:3m:4067)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN:3m:4066B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN:5j:4054)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN:1m:4059)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN:3m:4065B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN:12m:4077)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN:5j:4055B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (CtrlDEN:-8:4005B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN:1m:4058B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (CtrlDEN:-8:4003B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (CtrlDEN:-8:4001Bbis)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN:12m:4076)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN:1m:4062)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN:12m:4074)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN:1m:4061B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN:5j:4053)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN:1m:4060B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN:3m:4063)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN:12m:4073)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN:3m:4064)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (CtrlDEN:-8:4004B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Ctrl:9m:4027B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN:12m:4075)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Ctrl:9m:4026)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (CtrlDEN:-8:4002B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Ctrl:18mrev:4044)

description:
Liver sections from F344 rats treated with Clofibric Acid (CA)
Keywords = Clofibric-Acid
Keywords = DEN


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Ctrl:9m:4024)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Ctrl:18mrev:4046)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Ctrl:12mrev:4034)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Ctrl:18mrev:4043)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Ctrl:9m:4025)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Ctrl:9m:4023)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Ctrl:5j:4010B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Ctrl:12mrev:4032)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Ctrl:3m:4020B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Ctrl:12mrev:4035)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Ctrl:1m:4014)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Ctrl:12mrev:4033)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Ctrl:3m:4019)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Ctrl:3m:4016)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Ctrl:5j:4007B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Ctrl:18m:4045)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Ctrl:1m:4013B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Ctrl:1m:4015)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Ctrl:1m:4012B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Ctrl:3m:4018B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Ctrl:5j:4006B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Ctrl:1m:4011)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Ctrl:3m:4017)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Ctrl:5j:4008)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Ctrl:5j:4009B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Ctrl:18m:4036)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Ctrl:18m:4040)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Ctrl:12m:4037)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Ctrl:18m:4038)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Ctrl:18m:4039)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Ctrl:12m:4030)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Ctrl:12m:4028)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Ctrl:12m:4029)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Ctrl:12m:4031)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Clorev:18m:4151)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Clorev:18m:4151TUM)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Clorev:18m:4146)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Clo:9m:4134)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Clo:5j:4118)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Clo:9m:4135)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Clo:9m:4133)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Clo:5j:4121B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Clo:3m:4130)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Clo:3m:4131)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Clorev:18m:4149)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Clo:9m:4132)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Clo:5j:4120B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Clorev:18m:4148)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Clo:3m:4127)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Clo:9m:4136B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Clo:5j:4117)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Clo:3m:4128B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Clo:5j:4119)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Clo:1m:4124bis)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Clo:1m:4126)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Clo:1m:4122)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Clo:3m:4129)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month 4150tuga


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month 4150


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Clo:1m:4125B)


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month 4144


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month 4145


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month 4143 tumi


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month 4143


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month 4143 TUD2


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month 4143 peri


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month 4143 TUD1


title: Gene expression data from liver of rats treated with 5000 ppm CA for 18 month 4142 peri


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month 4144 tumi


title: Gene expression data from liver of rats treated with 5000 ppm CA for 12 month 4141 TUM


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month 4142


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month 4142 tuga


title: Gene expression data from liver of rats treated with 5000 ppm CA for 12 month 4141


title: Gene expression data from liver of rats treated with 5000 ppm CA for 12 month 4140


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month 4142 tumi


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month 4144 tuga


title: Gene expression data from liver of rats treated with 5000 ppm CA for 12 month 4137


title: CDV+TPA 96h /Ulatent_2

status: Public on Dec 01 2004

first: Michael

last: Lu

address: 10 Center Drive, Blg. 10, Rm 10S255

department: HIV and AIDS Malignancy Branch

institute: National Cancer Institute

description:
mAdb experiment ID: 40824
TPA induced BCBL1 with CDV treatment and harvested after 96 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-24
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: 100 microMolar
TPA Induction: 20 ng/ml
Induction Time (hrs): 96

organism: Human gammaherpesvirus 8

source_name: BCBL-1 Untreated Latent

source_name: BCBL-1 CDV-treated TPA induced 96hr


title: Gene expression data from liver of rats treated with 5000 ppm CA for 12 month 4138


title: CDV+TPA 96h /Ulatent_1

description:
mAdb experiment ID: 41438
TPA induced BCBL1 with CDV treatment and harvested after 96 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-55
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: 100 microMolar
TPA Induction: 20 ng/ml
Induction Time (hrs): 96


title: CDV+TPA 72h /Ulatent_3

description:
mAdb experiment ID: 47263
TPA induced BCBL1 with CDV treatment and harvested after 72 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-83
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: 100 microMolar
TPA Induction: 20 ng/ml
Induction Time (hrs): 72

source_name: BCBL-1 CDV-treated TPA induced 72hr


title: Gene expression data from liver of rats treated with 5000 ppm CA for 12 month 4137TUM


title: Gene expression data from liver of rats treated with 5000 ppm CA for 12 month 4137peri


title: Gene expression data from liver of rats treated with 5000 ppm CA for 12 month 4139


title: CDV+TPA 96h /Ulatent_3

description:
mAdb experiment ID: 47507
TPA induced BCBL1 with CDV treatment and harvested after 96 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-86
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: 100 microMolar
TPA Induction: 20 ng/ml
Induction Time (hrs): 96


title: CDV+TPA 72h /Ulatent_1

description:
mAdb experiment ID: 41437
TPA induced BCBL1 with CDV treatment and harvested after 72 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-54
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: 100 microMolar
TPA Induction: 20 ng/ml
Induction Time (hrs): 72


title: CDV+TPA 72h /Ulatent_2

description:
mAdb experiment ID: 40823
TPA induced BCBL1 with CDV treatment and harvested after 72 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-23
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: 100 microMolar
TPA Induction: 20 ng/ml
Induction Time (hrs): 72


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (DEN-Clo:1m:4092B)


title: CDV+TPA 48h /Ulatent_2

description:
mAdb experiment ID: 40822
TPA induced BCBL1 with CDV treatment and harvested after 48 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-22
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: 100 microMolar
TPA Induction: 20 ng/ml
Induction Time (hrs): 48

source_name: BCBL-1 CDV-treated TPA induced 48hr


title: CDV+TPA 48h /Ulatent_3

description:
mAdb experiment ID: 47262
TPA induced BCBL1 with CDV treatment and harvested after 48 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-82
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: 100 microMolar
TPA Induction: 20 ng/ml
Induction Time (hrs): 48


title: CDV+TPA 48h /Ulatent_1

description:
mAdb experiment ID: 41436
TPA induced BCBL1 with CDV treatment and harvested after 48 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-53
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: 100 microMolar
TPA Induction: 20 ng/ml
Induction Time (hrs): 48


title: CDV+TPA 36h /Ulatent_3

description:
mAdb experiment ID: 47261
TPA induced BCBL1 with CDV treatment and harvested after 36 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-81
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: 100 microMolar
TPA Induction: 20 ng/ml
Induction Time (hrs): 36

source_name: BCBL-1 CDV-treated TPA induced 36hr


title: CDV+TPA 12h /Ulatent_2

description:
mAdb experiment ID: 40844
TPA induced BCBL1 with CDV treatment and harvested after 12 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-13
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: 100 microMolar
TPA Induction: 20 ng/ml
Induction Time (hrs): 12

source_name: BCBL-1 CDV-treated TPA induced 12hr


title: CDV+TPA 12h /Ulatent_3

description:
mAdb experiment ID: 46743
TPA induced BCBL1 with CDV treatment and harvested after 12 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-75
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: 100 microMolar
TPA Induction: 20 ng/ml
Induction Time (hrs): 12


title: CDV+TPA 36h /Ulatent_1

description:
mAdb experiment ID: 41435
TPA induced BCBL1 with CDV treatment and harvested after 36 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-52
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: 100 microMolar
TPA Induction: 20 ng/ml
Induction Time (hrs): 36


title: CDV+TPA 24h /Ulatent_3

description:
mAdb experiment ID: 47260
TPA induced BCBL1 with CDV treatment and harvested after 24 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-80
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: 100 microMolar
TPA Induction: 20 ng/ml
Induction Time (hrs): 24

source_name: BCBL-1 CDV-treated TPA induced 24hr


title: CDV+TPA 10h /Ulatent_1

description:
mAdb experiment ID: 41345
TPA induced BCBL1 with CDV treatment and harvested after 10 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-44
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: 100 microMolar
TPA Induction: 20 ng/ml
Induction Time (hrs): 10

source_name: BCBL-1 CDV-treated TPA induced 10hr


title: CDV+TPA 24h /Ulatent_1

description:
mAdb experiment ID: 41434
TPA induced BCBL1 with CDV treatment and harvested after 24 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-51
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: 100 microMolar
TPA Induction: 20 ng/ml
Induction Time (hrs): 24


title: CDV+TPA 10h /Ulatent_2

description:
mAdb experiment ID: 40843
TPA induced BCBL1 with CDV treatment and harvested after 10 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-12
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: 100 microMolar
TPA Induction: 20 ng/ml
Induction Time (hrs): 10


title: UTPA 96h /Ulatent_1

description:
mAdb experiment ID: 41433
TPA induced BCBL1 without CDV treatment and harvested after 96 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-50
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: None
TPA Induction: 20 ng/ml
Induction Time (hrs): 96

source_name: BCBL-1 Untreated TPA induced 96hr


title: CDV+TPA 12h /Ulatent_1

description:
mAdb experiment ID: 41346
TPA induced BCBL1 with CDV treatment and harvested after 12 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-45
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: 100 microMolar
TPA Induction: 20 ng/ml
Induction Time (hrs): 12


title: CDV+TPA 8h /Ulatent_3

description:
mAdb experiment ID: 46741
TPA induced BCBL1 with CDV treatment and harvested after 8 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-73
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: 100 microMolar
TPA Induction: 20 ng/ml
Induction Time (hrs): 8

source_name: BCBL-1 CDV-treated TPA induced 8hr


title: CDV+TPA 8h /Ulatent_2

description:
mAdb experiment ID: 40841
TPA induced BCBL1 with CDV treatment and harvested after 8 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-11
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: 100 microMolar
TPA Induction: 20 ng/ml
Induction Time (hrs): 8


title: CDV+TPA 10h /Ulatent_3

description:
mAdb experiment ID: 46742
TPA induced BCBL1 with CDV treatment and harvested after 10 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-74
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: 100 microMolar
TPA Induction: 20 ng/ml
Induction Time (hrs): 10


title: CDV+TPA 6h /Ulatent_2

description:
mAdb experiment ID: 40840
TPA induced BCBL1 with CDV treatment and harvested after 6 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-10
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: 100 microMolar
TPA Induction: 20 ng/ml
Induction Time (hrs): 6

source_name: BCBL-1 CDV-treated TPA induced 6hr


title: CDV+TPA 6h /Ulatent_1

description:
mAdb experiment ID: 41343
TPA induced BCBL1 with CDV treatment and harvested after 6 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-42
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: 100 microMolar
TPA Induction: 20 ng/ml
Induction Time (hrs): 6


title: CVV+TPA 6h /Ulatent_3

description:
mAdb experiment ID: 46740
TPA induced BCBL1 with CDV treatment and harvested after 6 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-72
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: 100 microMolar
TPA Induction: 20 ng/ml
Induction Time (hrs): 6


title: CDV+TPA 36h /Ulatent_2

description:
mAdb experiment ID: 40821
TPA induced BCBL1 with CDV treatment and harvested after 36 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-21
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: 100 microMolar
TPA Induction: 20 ng/ml
Induction Time (hrs): 36


title: UTPA 72h /Ulatent_3

description:
mAdb experiment ID: 47259
TPA induced BCBL1 without CDV treatment and harvested after 72 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-79
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: None
TPA Induction: 20 ng/ml
Induction Time (hrs): 72

source_name: BCBL-1 Untreated TPA induced 72hr


title: UTPA 96h /Ulatent_3

description:
mAdb experiment ID: 47248
TPA induced BCBL1 without CDV treatment and harvested after 96 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-85
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: None
TPA Induction: 20 ng/ml
Induction Time (hrs): 96


title: UTPA 96h /Ulatent_2

description:
mAdb experiment ID: 40819
TPA induced BCBL1 without CDV treatment and harvested after 96 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-19
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: None
TPA Induction: 20 ng/ml
Induction Time (hrs): 96


title: CDV+TPA 0h /Ulatent_1

description:
mAdb experiment ID: 41342
TPA induced BCBL1 with CDV treatment and harvested after 0 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-41
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: 100 microMolar
TPA Induction: 20 ng/ml
Induction Time (hrs): 0

source_name: BCBL-1 CDV-treated TPA induced 0hr


title: CDV+TPA 0h /Ulatent_3

description:
mAdb experiment ID: 47247
TPA induced BCBL1 with CDV treatment and harvested after 0 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-84
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: 100 microMolar
TPA Induction: 20 ng/ml
Induction Time (hrs): 0


title: CDV+TPA 8h /Ulatent_1

description:
mAdb experiment ID: 41344
TPA induced BCBL1 with CDV treatment and harvested after 8 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-43
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: 100 microMolar
TPA Induction: 20 ng/ml
Induction Time (hrs): 8


title: CDV+TPA 0h /Ulatent_2

description:
mAdb experiment ID: 40839
TPA induced BCBL1 with CDV treatment and harvested after 0 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-09
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: 100 microMolar
TPA Induction: 20 ng/ml
Induction Time (hrs): 0


title: UTPA 72h /Ulatent_2

description:
mAdb experiment ID: 40818
TPA induced BCBL1 without CDV treatment and harvested after 72 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-18
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: None
TPA Induction: 20 ng/ml
Induction Time (hrs): 72


title: UTPA 72h /Ulatent_1

description:
mAdb experiment ID: 41432
TPA induced BCBL1 without CDV treatment and harvested after 72 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-49
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: None
TPA Induction: 20 ng/ml
Induction Time (hrs): 72


title: UTPA 48h /Ulatent_2

description:
mAdb experiment ID: 40817
TPA induced BCBL1 without CDV treatment and harvested after 48 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-16
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: None
TPA Induction: 20 ng/ml
Induction Time (hrs): 48

source_name: BCBL-1 Untreated TPA induced 48hr


title: UTPA 48h /Ulatent_3

description:
mAdb experiment ID: 47258
TPA induced BCBL1 without CDV treatment and harvested after 48 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-78
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: None
TPA Induction: 20 ng/ml
Induction Time (hrs): 48


title: UTPA 36h /Ulatent_2

description:
mAdb experiment ID: 40816
TPA induced BCBL1 without CDV treatment and harvested after 36 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-15
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: None
TPA Induction: 20 ng/ml
Induction Time (hrs): 36

source_name: BCBL-1 Untreated TPA induced 36hr


title: UTPA 48h /Ulatent_1

description:
mAdb experiment ID: 41431
TPA induced BCBL1 without CDV treatment and harvested after 48 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-48
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: None
TPA Induction: 20 ng/ml
Induction Time (hrs): 48


title: UTPA 24h /Ulatent_3

description:
mAdb experiment ID: 47256
TPA induced BCBL1 without CDV treatment and harvested after 24 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-76
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: None
TPA Induction: 20 ng/ml
Induction Time (hrs): 24

source_name: BCBL-1 Untreated TPA induced 24hr


title: UTPA 12h /Ulatent_1

description:
mAdb experiment ID: 41331
TPA induced BCBL1 without CDV treatment and harvested after 12 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-40
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: None
TPA Induction: 20 ng/ml
Induction Time (hrs): 12

source_name: BCBL-1 Untreated TPA induced 12hr


title: UTPA 36h /Ulatent_3

description:
mAdb experiment ID: 47257
TPA induced BCBL1 without CDV treatment and harvested after 36 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-77
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: None
TPA Induction: 20 ng/ml
Induction Time (hrs): 36

source_name: BCBL-1 Untreated Latent


title: UTPA 36h /Ulatent_1

description:
mAdb experiment ID: 41430
TPA induced BCBL1 without CDV treatment and harvested after 36 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-47
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: None
TPA Induction: 20 ng/ml
Induction Time (hrs): 36


title: UTPA 24h /Ulatent_2

description:
mAdb experiment ID: 40815
TPA induced BCBL1 without CDV treatment and harvested after 24 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-14
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: None
TPA Induction: 20 ng/ml
Induction Time (hrs): 24


title: UTPA 24h /Ulatent_1

description:
mAdb experiment ID: 41429
TPA induced BCBL1 without CDV treatment and harvested after 24 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-46
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: None
TPA Induction: 20 ng/ml
Induction Time (hrs): 24


title: UTPA 10h /Ulatent_1

description:
mAdb experiment ID: 41330
TPA induced BCBL1 without CDV treatment and harvested after 10 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-39
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: None
TPA Induction: 20 ng/ml
Induction Time (hrs): 10

source_name: BCBL-1 Untreated TPA induced 10hr


title: UTPA 8h /Ulatent_3

description:
mAdb experiment ID: 46737
TPA induced BCBL1 without CDV treatment and harvested after 8 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-69
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: None
TPA Induction: 20 ng/ml
Induction Time (hrs): 8

source_name: BCBL-1 Untreated TPA induced 8hr


title: UTPA 8h /Ulatent_2

description:
mAdb experiment ID: 40837
TPA induced BCBL1 without CDV treatment and harvested after 8 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-07
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: None
TPA Induction: 20 ng/ml
Induction Time (hrs): 8


title: UTPA 10h /Ulatent_3

description:
mAdb experiment ID: 46738
TPA induced BCBL1 without CDV treatment and harvested after 10 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-70
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: None
TPA Induction: 20 ng/ml
Induction Time (hrs): 10


title: UTPA 0h /Ulatent_2

description:
mAdb experiment ID: 40835
TPA induced BCBL1 without CDV treatment and harvested after 0 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-05
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: None
TPA Induction: 20 ng/ml
Induction Time (hrs): 0

source_name: BCBL-1 Untreated TPA induced 0hr


title: UTPA 6h /Ulatent_3

description:
mAdb experiment ID: 46736
TPA induced BCBL1 without CDV treatment and harvested after 6 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-68
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: None
TPA Induction: 20 ng/ml
Induction Time (hrs): 6

source_name: BCBL-1 Untreated TPA induced 6hr


title: UTPA 6h /Ulatent_1

description:
mAdb experiment ID: 41328
TPA induced BCBL1 without CDV treatment and harvested after 6 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-37
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: None
TPA Induction: 20 ng/ml
Induction Time (hrs): 6


title: UTPA 12h /Ulatent_2

description:
mAdb experiment ID: 41181
TPA induced BCBL1 without CDV treatment and harvested after 12 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-30
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: None
TPA Induction: 20 ng/ml
Induction Time (hrs): 12


title: UTPA 0h /Ulatent_1

description:
mAdb experiment ID: 41327
TPA induced BCBL1 without CDV treatment and harvested after 0 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-36
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: None
TPA Induction: 20 ng/ml
Induction Time (hrs): 0


title: UTPA 6h /Ulatent_2

description:
mAdb experiment ID: 40836
TPA induced BCBL1 without CDV treatment and harvested after 6 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-06
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: None
TPA Induction: 20 ng/ml
Induction Time (hrs): 6


title: UTPA 12h /Ulatent_3

description:
mAdb experiment ID: 46739
TPA induced BCBL1 without CDV treatment and harvested after 12 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-71
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: None
TPA Induction: 20 ng/ml
Induction Time (hrs): 12


title: UTPA 8h /Ulatent_1

description:
mAdb experiment ID: 41329
TPA induced BCBL1 without CDV treatment and harvested after 8 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-38
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: None
TPA Induction: 20 ng/ml
Induction Time (hrs): 8


title: UTPA 10h /Ulatent_2

description:
mAdb experiment ID: 40838
TPA induced BCBL1 without CDV treatment and harvested after 10 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-08
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: None
TPA Induction: 20 ng/ml
Induction Time (hrs): 10


title: UTPA 0h /Ulatent_3

description:
mAdb experiment ID: 47246
TPA induced BCBL1 without CDV treatment and harvested after 0 hrs versus untreated Latent BCBL-1 cells
Organism synonym: KSHV
Array Print: hhv8v2p4-67
Cell line origin: lymphocytic
Cell Line: BCBL-1
CDV Treatment: None
TPA Induction: 20 ng/ml
Induction Time (hrs): 0


title: T cells- BDC2.5NOD pancreas-rep3

status: Public on Apr 17 2004

city: Boston

first: CBDM

last: Lab

state: MA

address: 77 Avenue Louis Pasteur

department: Microbiology and Immunobiology

institute: Harvard Medical School

description:
Antigen-specific T cells from the pancreatic infiltrate of mice developing a benign insulitis (BDC2.5-NOD at 25 days of age)
jos1743

data_processing: RMA

source_name: pancreas

extract_protocol: RNA isolated with Trizol reagent and amplified twice

treatment_protocol: No treatment

tag: Genetic Background

value: NOD.

tag: Transgene:BDC2.5 T cell receptor genes.

tag: Age:25 days.

value: pancreas.

tag: Isolation

value: FACS on CD45+, CD4+, BDC2.5 clonotype high, Hoechst- (viable) cells


title: Splenic leukocytes-NOD-Rep2

description:
Total leukocytes from NOD spleen. Serves as reference for leukocytes isolated from pancreatic infiltrates of BDC2.5 mice on NOD genetic background
jos1767

source_name: spleen

extract_protocol: RNA isolated with Trizol reagent and subject to one round of amplification.

tag: Age:6 weeks.

value: Spleen.

value: FACS on CD45+ Hoechst- (viable) cells.


title: Splenic leukocytes-NOD-rep1

description:
Total leukocytes from NOD spleen. Serves as reference for leukocytes isolated from pancreatic infiltrates of BDC2.5 mice on NOD genetic background
jos1765

tag: Age:5 weeks.


title: T cells- BDC2.5NOD pancreas-rep1

description:
Antigen-specific T cells from the pancreatic infiltrate of mice developing a benign insulitis (BDC2.5-NOD at 24 days of age)
jos1143

tag: Age:24 days.

tag: Tissue


title: T cells- BDC2.5NOD pancreas-rep2

description:
Antigen-specific T cells from the pancreatic infiltrate of mice developing a benign insulitis (BDC2.5-NOD at 24 days of age)
jos1264


title: NK cells-NOD-rep1

description:
Natural killer cells from mice on NOD genetic background.
jos1783

extract_protocol: RNA isolated with Trizol reagent and amplified twice.

value: spleen.

value: FACS on CD45+ AsialoGM1+ Hoechst- (viable) cells.


title: NK cells-NOD-rep2

description:
Natural killer cells from mice on NOD genetic background with BDC2.5 transgenic T-cell receptor.
jos1785

tag: Transgene:BDC2.5 Tg TCR


title: T cells-BDC2.5/NOD lymph node-rep3

description:
Antigen specific T cells isolated from the pancreatic lymph nodes of mice developing a benign insulitis (BDC2.5-NOD at 25 days of age)
jos1741

source_name: pancreatic lymph nodes

tag: Age:25 days

value: pancreatic lymph nodes

value: FACS on CD4+, BDC2.5 clonotype high, Hoechst- (viable) cells


title: T cells-BDC2.5/NOD lymph node-rep1

description:
Antigen specific T cells isolated from the pancreatic lymph nodes of mice developing a benign insulitis (BDC2.5-NOD at 24 days of age)
jos1141

tag: Age:24 days


title: Total pancreatic infiltrate- BDC2.5 NOD-rep3

description:
Total leukocytic infiltrate from mice developing a benign insulitis (BDC2.5NOD at 25 days of age)
jos1426

value: pancreas

value: FACS on CD45+ Hoechst- (viable) cells


title: T cells-BDC2.5/B6g7 pancreas-rep2

description:
Antigen-specific pancreatic T cells from mice developing an aggressive insulitis (BDC2.5-B6g7 at 22 days of age)
jos1637

extract_protocol: RNA isolated using Trizol reagent and amplified twice.

tag: Genetic background

value: C57Bl/6.H2g7/g7. Transgene:BDC2.5 T cell receptor genes. Tissue:pancreas Isolation:FACS on CD45+, CD4+, BDC2.5 clonotype high, Hoechst- (viable) cells. Age:22 days of age


title: Total pancreatic infiltrate- BDC2.5 NOD-rep2

description:
Total leukocytic infiltrate from mice developing a benign insulitis (BDC2.5NOD at 25 days of age)
jos1280


title: Splenic Leukocytes-B6g7-rep2

description:
Total leukocytes from C57Bl/6-H2g7/g7 spleen. Serves as reference for leukocytes isolated from pancreatic infiltrates of BDC2.5 mice on C57Bl/6-H2g7/g7 genetic background.
jos1766


title: Total pancreatic infiltrate- BDC2.5 NOD-rep1

description:
Total leukocytic infiltrate from mice developing a benign insulitis (BDC2.5NOD at 25 days of age)
jos1279


title: Splenic Leukocytes-B6g7-rep1

description:
Total leukocytes from C57Bl/6-H2g7/g7 spleen. Serves as reference for leukocytes isolated from pancreatic infiltrates of BDC2.5 mice on C57Bl/6-H2g7/g7 genetic background.
jos1768

treatment_protocol: No treatment.


title: Total pancreatic infiltrate- BDC2.5 B6g7-rep2

description: Total leukocytic infiltrate from mice developing an aggressive insulitis (BDC2.5B6g7 at 28 days of age).  jos1322

source_name: BDC2.5/B6g7 pancreatic leukocytes

tag: Age:28 days


title: Total pancreatic infiltrate- BDC2.5 B6g7-rep1

description:
Total leukocytic infiltrate from mice developping an aggressive insulitis (BDC2.5B6g7 at 25 days of age)

jos1281


title: Strain M34, Rich media, Replicate 3

status: Public on Mar 01 2004

city: Saint Louis

first: Justin

last: Fay

state: MO

address: 4566 Scott Ave, Box 8232

department: Genetics

institute: Washington University

description:
Strain M34 during exponential growth in Rich media, Replicate 3.
The reference sample is the combined RNA from all 9 strains.
RNA was extrated using hot phenol-chloroform, reverse transcribed using amino-allyle dUTP and labelled with either Cy3 or Cy5 flourescent dye.
Images were scanned and spot intensities extracted using a GenePix 4000A scanner and GenePix 4.0 software. Bad spots were flagged based on the image.
Sample_keywork: Rich media

source_name: Strain M34, Rich media, Replicate 3

source_name: Rich media reference, Rich media, Replicate 3


title: T cells-BDC2.5/B6g7 pancreas-rep1

description:
Antigen-specific pancreatic T cells from mice developping an aggressive insulitis (BDC2.5-B6g7 at 25 days of age)
jos1157

tag: Transgene

value: BDC2.5 T cell receptor genes.

value: FACS on CD45+, CD4+, BDC2.5 clonotype high, Hoechst- (viable) cells.

tag: Age:25 days of age


description:
reference during exponential growth in Rich media or copper sulfate, Replicate 5.
The reference sample is the combined RNA from all 9 strains.
RNA was extrated using hot phenol-chloroform, reverse transcribed using amino-allyle dUTP and labelled with either Cy3 or Cy5 flourescent dye.
Images were scanned and spot intensities extracted using a GenePix 4000A scanner and GenePix 4.0 software. Bad spots were flagged based on the image.
Sample_keywork: Rich media or copper sulfate

source_name: Rich media reference, Rich media or copper sulfate, Replicate 5

source_name: Copper sulfate reference, Rich media or copper sulfate, Replicate 5


description:
reference during exponential growth in Rich media or copper sulfate, Replicate 2.
The reference sample is the combined RNA from all 9 strains.
RNA was extrated using hot phenol-chloroform, reverse transcribed using amino-allyle dUTP and labelled with either Cy3 or Cy5 flourescent dye.
Images were scanned and spot intensities extracted using a GenePix 4000A scanner and GenePix 4.0 software. Bad spots were flagged based on the image.
Sample_keywork: Rich media or copper sulfate

source_name: Rich media reference, Rich media or copper sulfate, Replicate 2

source_name: Copper sulfate reference, Rich media or copper sulfate, Replicate 2


description:
reference during exponential growth in Rich media or copper sulfate, Replicate 4.
The reference sample is the combined RNA from all 9 strains.
RNA was extrated using hot phenol-chloroform, reverse transcribed using amino-allyle dUTP and labelled with either Cy3 or Cy5 flourescent dye.
Images were scanned and spot intensities extracted using a GenePix 4000A scanner and GenePix 4.0 software. Bad spots were flagged based on the image.
Sample_keywork: Rich media or copper sulfate

source_name: Rich media reference, Rich media or copper sulfate, Replicate 4

source_name: Copper sulfate reference, Rich media or copper sulfate, Replicate 4


description:
reference during exponential growth in Rich media or copper sulfate, Replicate 6.
The reference sample is the combined RNA from all 9 strains.
RNA was extrated using hot phenol-chloroform, reverse transcribed using amino-allyle dUTP and labelled with either Cy3 or Cy5 flourescent dye.
Images were scanned and spot intensities extracted using a GenePix 4000A scanner and GenePix 4.0 software. Bad spots were flagged based on the image.
Sample_keywork: Rich media or copper sulfate

source_name: Rich media reference, Rich media or copper sulfate, Replicate 6

source_name: Copper sulfate reference, Rich media or copper sulfate, Replicate 6


description:
reference during exponential growth in Rich media or copper sulfate, Replicate 3.
The reference sample is the combined RNA from all 9 strains.
RNA was extrated using hot phenol-chloroform, reverse transcribed using amino-allyle dUTP and labelled with either Cy3 or Cy5 flourescent dye.
Images were scanned and spot intensities extracted using a GenePix 4000A scanner and GenePix 4.0 software. Bad spots were flagged based on the image.
Sample_keywork: Rich media or copper sulfate

source_name: Copper sulfate reference, Rich media or copper sulfate, Replicate 3

source_name: Rich media reference, Rich media or copper sulfate, Replicate 3


title: Strain M22, Rich media, Replicate 3

description:
Strain M22 during exponential growth in Rich media, Replicate 3.
The reference sample is the combined RNA from all 9 strains.
RNA was extrated using hot phenol-chloroform, reverse transcribed using amino-allyle dUTP and labelled with either Cy3 or Cy5 flourescent dye.
Images were scanned and spot intensities extracted using a GenePix 4000A scanner and GenePix 4.0 software. Bad spots were flagged based on the image.
Sample_keywork: Rich media

source_name: Strain M22, Rich media, Replicate 3


title: Strain M8, Rich media, Replicate 3

description:
Strain M8 during exponential growth in Rich media, Replicate 3.
The reference sample is the combined RNA from all 9 strains.
RNA was extrated using hot phenol-chloroform, reverse transcribed using amino-allyle dUTP and labelled with either Cy3 or Cy5 flourescent dye.
Images were scanned and spot intensities extracted using a GenePix 4000A scanner and GenePix 4.0 software. Bad spots were flagged based on the image.
Sample_keywork: Rich media

source_name: Strain M8, Rich media, Replicate 3


title: Total pancreatic infiltrate- BDC2.5 B6g7-rep3

description: Total leukocytic infiltrate from mice developing an aggressive insulitis (BDC2.5B6g7 at 24 days of age).  jos1425

source_name: BDC2.5/b6g7 pancreatic leukocytes

growth_protocol: RNA isolated with Trizol reagent and amplified twice


description:
reference during exponential growth in Rich media or copper sulfate, Replicate 1.
The reference sample is the combined RNA from all 9 strains.
RNA was extrated using hot phenol-chloroform, reverse transcribed using amino-allyle dUTP and labelled with either Cy3 or Cy5 flourescent dye.
Images were scanned and spot intensities extracted using a GenePix 4000A scanner and GenePix 4.0 software. Bad spots were flagged based on the image.
Sample_keywork: Rich media or copper sulfate

source_name: Copper sulfate reference, Rich media or copper sulfate, Replicate 1

source_name: Rich media reference, Rich media or copper sulfate, Replicate 1


title: Strain S288C, Rich media, Replicate 3

description:
Strain S288C during exponential growth in Rich media, Replicate 3.
The reference sample is the combined RNA from all 9 strains.
RNA was extrated using hot phenol-chloroform, reverse transcribed using amino-allyle dUTP and labelled with either Cy3 or Cy5 flourescent dye.
Images were scanned and spot intensities extracted using a GenePix 4000A scanner and GenePix 4.0 software. Bad spots were flagged based on the image.
Sample_keywork: Rich media

source_name: Strain S288C, Rich media, Replicate 3


title: Gene expression data from liver of rats treated with 5000 ppm CA for X month (Clo:1m:4123)

description:
Liver sections from F344 rats treated with Clofibric Acid (CA)
Keywords = Clofibric-Acid, DEN


title: Strain M13, Rich media, Replicate 3

description:
Strain M13 during exponential growth in Rich media, Replicate 3.
The reference sample is the combined RNA from all 9 strains.
RNA was extrated using hot phenol-chloroform, reverse transcribed using amino-allyle dUTP and labelled with either Cy3 or Cy5 flourescent dye.
Images were scanned and spot intensities extracted using a GenePix 4000A scanner and GenePix 4.0 software. Bad spots were flagged based on the image.
Sample_keywork: Rich media

source_name: Strain M13, Rich media, Replicate 3

source_name: Rich media reference, Rich media, Replicate 3


title: Strain YPS163, Rich media, Replicate 3

description:
Strain YPS163 during exponential growth in Rich media, Replicate 3.
The reference sample is the combined RNA from all 9 strains.
RNA was extrated using hot phenol-chloroform, reverse transcribed using amino-allyle dUTP and labelled with either Cy3 or Cy5 flourescent dye.
Images were scanned and spot intensities extracted using a GenePix 4000A scanner and GenePix 4.0 software. Bad spots were flagged based on the image.
Sample_keywork: Rich media

source_name: Strain YPS163, Rich media, Replicate 3


title: Strain M32, Rich media, Replicate 3

description:
Strain M32 during exponential growth in Rich media, Replicate 3.
The reference sample is the combined RNA from all 9 strains.
RNA was extrated using hot phenol-chloroform, reverse transcribed using amino-allyle dUTP and labelled with either Cy3 or Cy5 flourescent dye.
Images were scanned and spot intensities extracted using a GenePix 4000A scanner and GenePix 4.0 software. Bad spots were flagged based on the image.
Sample_keywork: Rich media

source_name: Strain M32, Rich media, Replicate 3


title: Strain M14, Rich media, Replicate 3

description:
Strain M14 during exponential growth in Rich media, Replicate 3.
The reference sample is the combined RNA from all 9 strains.
RNA was extrated using hot phenol-chloroform, reverse transcribed using amino-allyle dUTP and labelled with either Cy3 or Cy5 flourescent dye.
Images were scanned and spot intensities extracted using a GenePix 4000A scanner and GenePix 4.0 software. Bad spots were flagged based on the image.
Sample_keywork: Rich media

source_name: Strain M14, Rich media, Replicate 3


title: Strain M34, Rich media, Replicate 2

description:
Strain M34 during exponential growth in Rich media, Replicate 2.
The reference sample is the combined RNA from all 9 strains.
RNA was extrated using hot phenol-chloroform, reverse transcribed using amino-allyle dUTP and labelled with either Cy3 or Cy5 flourescent dye.
Images were scanned and spot intensities extracted using a GenePix 4000A scanner and GenePix 4.0 software. Bad spots were flagged based on the image.
Sample_keywork: Rich media

source_name: Strain M34, Rich media, Replicate 2

source_name: Rich media reference, Rich media, Replicate 2


title: Strain M5, Rich media, Replicate 3

description:
Strain M5 during exponential growth in Rich media, Replicate 3.
The reference sample is the combined RNA from all 9 strains.
RNA was extrated using hot phenol-chloroform, reverse transcribed using amino-allyle dUTP and labelled with either Cy3 or Cy5 flourescent dye.
Images were scanned and spot intensities extracted using a GenePix 4000A scanner and GenePix 4.0 software. Bad spots were flagged based on the image.
Sample_keywork: Rich media

source_name: Strain M5, Rich media, Replicate 3


title: Strain M8, Rich media, Replicate 2

description:
Strain M8 during exponential growth in Rich media, Replicate 2.
The reference sample is the combined RNA from all 9 strains.
RNA was extrated using hot phenol-chloroform, reverse transcribed using amino-allyle dUTP and labelled with either Cy3 or Cy5 flourescent dye.
Images were scanned and spot intensities extracted using a GenePix 4000A scanner and GenePix 4.0 software. Bad spots were flagged based on the image.
Sample_keywork: Rich media

source_name: Strain M8, Rich media, Replicate 2


title: Strain YPS163, Rich media, Replicate 2

description:
Strain YPS163 during exponential growth in Rich media, Replicate 2.
The reference sample is the combined RNA from all 9 strains.
RNA was extrated using hot phenol-chloroform, reverse transcribed using amino-allyle dUTP and labelled with either Cy3 or Cy5 flourescent dye.
Images were scanned and spot intensities extracted using a GenePix 4000A scanner and GenePix 4.0 software. Bad spots were flagged based on the image.
Sample_keywork: Rich media

source_name: Strain YPS163, Rich media, Replicate 2


title: Strain S288C, Rich media, Replicate 2

description:
Strain S288C during exponential growth in Rich media, Replicate 2.
The reference sample is the combined RNA from all 9 strains.
RNA was extrated using hot phenol-chloroform, reverse transcribed using amino-allyle dUTP and labelled with either Cy3 or Cy5 flourescent dye.
Images were scanned and spot intensities extracted using a GenePix 4000A scanner and GenePix 4.0 software. Bad spots were flagged based on the image.
Sample_keywork: Rich media

source_name: Strain S288C, Rich media, Replicate 2


title: Strain M14, Rich media, Replicate 2

description:
Strain M14 during exponential growth in Rich media, Replicate 2.
The reference sample is the combined RNA from all 9 strains.
RNA was extrated using hot phenol-chloroform, reverse transcribed using amino-allyle dUTP and labelled with either Cy3 or Cy5 flourescent dye.
Images were scanned and spot intensities extracted using a GenePix 4000A scanner and GenePix 4.0 software. Bad spots were flagged based on the image.
Sample_keywork: Rich media

source_name: Strain M14, Rich media, Replicate 2


title: Strain M13, Rich media, Replicate 2

description:
Strain M13 during exponential growth in Rich media, Replicate 2.
The reference sample is the combined RNA from all 9 strains.
RNA was extrated using hot phenol-chloroform, reverse transcribed using amino-allyle dUTP and labelled with either Cy3 or Cy5 flourescent dye.
Images were scanned and spot intensities extracted using a GenePix 4000A scanner and GenePix 4.0 software. Bad spots were flagged based on the image.
Sample_keywork: Rich media

source_name: Strain M13, Rich media, Replicate 2


title: Strain M22, Rich media, Replicate 2

description:
Strain M22 during exponential growth in Rich media, Replicate 2.
The reference sample is the combined RNA from all 9 strains.
RNA was extrated using hot phenol-chloroform, reverse transcribed using amino-allyle dUTP and labelled with either Cy3 or Cy5 flourescent dye.
Images were scanned and spot intensities extracted using a GenePix 4000A scanner and GenePix 4.0 software. Bad spots were flagged based on the image.
Sample_keywork: Rich media

source_name: Strain M22, Rich media, Replicate 2


title: Strain M5, Rich media, Replicate 2

description:
Strain M5 during exponential growth in Rich media, Replicate 2.
The reference sample is the combined RNA from all 9 strains.
RNA was extrated using hot phenol-chloroform, reverse transcribed using amino-allyle dUTP and labelled with either Cy3 or Cy5 flourescent dye.
Images were scanned and spot intensities extracted using a GenePix 4000A scanner and GenePix 4.0 software. Bad spots were flagged based on the image.
Sample_keywork: Rich media

source_name: Strain M5, Rich media, Replicate 2


title: Strain M32, Rich media, Replicate 2

description:
Strain M32 during exponential growth in Rich media, Replicate 2.
The reference sample is the combined RNA from all 9 strains.
RNA was extrated using hot phenol-chloroform, reverse transcribed using amino-allyle dUTP and labelled with either Cy3 or Cy5 flourescent dye.
Images were scanned and spot intensities extracted using a GenePix 4000A scanner and GenePix 4.0 software. Bad spots were flagged based on the image.
Sample_keywork: Rich media

source_name: Strain M32, Rich media, Replicate 2


title: Strain M5, Rich media, Replicate 1

description:
Strain M5 during exponential growth in Rich media, Replicate 1.
The reference sample is the combined RNA from all 9 strains.
RNA was extrated using hot phenol-chloroform, reverse transcribed using amino-allyle dUTP and labelled with either Cy3 or Cy5 flourescent dye.
Images were scanned and spot intensities extracted using a GenePix 4000A scanner and GenePix 4.0 software. Bad spots were flagged based on the image.
Sample_keywork: Rich media

source_name: Rich media reference, Rich media, Replicate 1

source_name: Strain M5, Rich media, Replicate 1


title: Strain M34, Rich media, Replicate 1

description:
Strain M34 during exponential growth in Rich media, Replicate 1.
The reference sample is the combined RNA from all 9 strains.
RNA was extrated using hot phenol-chloroform, reverse transcribed using amino-allyle dUTP and labelled with either Cy3 or Cy5 flourescent dye.
Images were scanned and spot intensities extracted using a GenePix 4000A scanner and GenePix 4.0 software. Bad spots were flagged based on the image.
Sample_keywork: Rich media

source_name: Strain M34, Rich media, Replicate 1


title: Strain M22, Rich media, Replicate 1

description:
Strain M22 during exponential growth in Rich media, Replicate 1.
The reference sample is the combined RNA from all 9 strains.
RNA was extrated using hot phenol-chloroform, reverse transcribed using amino-allyle dUTP and labelled with either Cy3 or Cy5 flourescent dye.
Images were scanned and spot intensities extracted using a GenePix 4000A scanner and GenePix 4.0 software. Bad spots were flagged based on the image.
Sample_keywork: Rich media

source_name: Strain M22, Rich media, Replicate 1


title: Strain M34, Copper sulfate media, Replicate 3

description:
Strain M34 during exponential growth in Copper sulfate media, Replicate 3.
The reference sample is the combined RNA from all 9 strains.
RNA was extrated using hot phenol-chloroform, reverse transcribed using amino-allyle dUTP and labelled with either Cy3 or Cy5 flourescent dye.
Images were scanned and spot intensities extracted using a GenePix 4000A scanner and GenePix 4.0 software. Bad spots were flagged based on the image.
Sample_keywork: Copper sulfate media

source_name: Copper sulfate reference, Copper sulfate media, Replicate 3


title: Strain YPS163, Copper sulfate media, Replicate 3

description:
Strain YPS163 during exponential growth in Copper sulfate media, Replicate 3.
The reference sample is the combined RNA from all 9 strains.
RNA was extrated using hot phenol-chloroform, reverse transcribed using amino-allyle dUTP and labelled with either Cy3 or Cy5 flourescent dye.
Images were scanned and spot intensities extracted using a GenePix 4000A scanner and GenePix 4.0 software. Bad spots were flagged based on the image.
Sample_keywork: Copper sulfate media


title: Strain M13, Rich media, Replicate 1

description:
Strain M13 during exponential growth in Rich media, Replicate 1.
The reference sample is the combined RNA from all 9 strains.
RNA was extrated using hot phenol-chloroform, reverse transcribed using amino-allyle dUTP and labelled with either Cy3 or Cy5 flourescent dye.
Images were scanned and spot intensities extracted using a GenePix 4000A scanner and GenePix 4.0 software. Bad spots were flagged based on the image.
Sample_keywork: Rich media

source_name: Strain M13, Rich media, Replicate 1


title: Strain M8, Rich media, Replicate 1

description:
Strain M8 during exponential growth in Rich media, Replicate 1.
The reference sample is the combined RNA from all 9 strains.
RNA was extrated using hot phenol-chloroform, reverse transcribed using amino-allyle dUTP and labelled with either Cy3 or Cy5 flourescent dye.
Images were scanned and spot intensities extracted using a GenePix 4000A scanner and GenePix 4.0 software. Bad spots were flagged based on the image.
Sample_keywork: Rich media

source_name: Strain M8, Rich media, Replicate 1


title: Strain M14, Copper sulfate media, Replicate 3

description:
Strain M14 during exponential growth in Copper sulfate media, Replicate 3.
The reference sample is the combined RNA from all 9 strains.
RNA was extrated using hot phenol-chloroform, reverse transcribed using amino-allyle dUTP and labelled with either Cy3 or Cy5 flourescent dye.
Images were scanned and spot intensities extracted using a GenePix 4000A scanner and GenePix 4.0 software. Bad spots were flagged based on the image.
Sample_keywork: Copper sulfate media


title: Strain M32, Rich media, Replicate 1

description:
Strain M32 during exponential growth in Rich media, Replicate 1.
The reference sample is the combined RNA from all 9 strains.
RNA was extrated using hot phenol-chloroform, reverse transcribed using amino-allyle dUTP and labelled with either Cy3 or Cy5 flourescent dye.
Images were scanned and spot intensities extracted using a GenePix 4000A scanner and GenePix 4.0 software. Bad spots were flagged based on the image.
Sample_keywork: Rich media

source_name: Strain M32, Rich media, Replicate 1


title: Strain S288C, Copper sulfate media, Replicate 3

description:
Strain S288C during exponential growth in Copper sulfate media, Replicate 3.
The reference sample is the combined RNA from all 9 strains.
RNA was extrated using hot phenol-chloroform, reverse transcribed using amino-allyle dUTP and labelled with either Cy3 or Cy5 flourescent dye.
Images were scanned and spot intensities extracted using a GenePix 4000A scanner and GenePix 4.0 software. Bad spots were flagged based on the image.
Sample_keywork: Copper sulfate media


title: Strain S288C, Rich media, Replicate 1

description:
Strain S288C during exponential growth in Rich media, Replicate 1.
The reference sample is the combined RNA from all 9 strains.
RNA was extrated using hot phenol-chloroform, reverse transcribed using amino-allyle dUTP and labelled with either Cy3 or Cy5 flourescent dye.
Images were scanned and spot intensities extracted using a GenePix 4000A scanner and GenePix 4.0 software. Bad spots were flagged based on the image.
Sample_keywork: Rich media

source_name: Strain S288C, Rich media, Replicate 1