title: MGAS315 7-9 St -1

status: Public on Jun 28 2007

type: RNA

city: Atlanta

first: June

last: Scott

state: GA

address: 1510 Clifton Road

department: Microbiology and Immunology

country: USA

institute: Emory University

description: Total RNA was isolated by CsCl2 method and labeled cDNA was generated using the Fairplay microarray labelling kit from Stratagene using random hexamers.  Labeled cDNA was purified using QiaQuick PCR purification columns from Qiagen and concentrated using Microcon YM30 columns from Amicon.  The amount of incorporated label was determined by measuring the absorbance at 550nm (Cy3) or 650nm (Cy5). 60 pmol each of Cy-dye (reference cDNA (Cy5) and test cDNA (Cy3)) were combined in 60 ml hybridization buffer 1 (Ambion), denatured and hybridized to the array at 42�C for at least 16 h, followed by subsequent washes.  Output data (F635 mean-B635, F532 mean-B532, flags) was filtered to set any spots that did not exceed 2SD above a background threshold to the threshold value to avoid spurious expression ratios. Remaining spots were averaged, filtered to remove genes not present in MGAS315 (the strain examined) and normalized before statistical analysis.

data_processing:
Growth phase specific normalization after removing genes not present in MGAS315.
Condition-specific normalization:
Following data filtration and ratio of signal calculations, the data from each array was adjusted and normalized so that valid comparisons could be made between experiments. Because the total level of transcript in the conditions being compared (stationary vs. exponential phase) was not similar, we compared the relative abundance of the transcript in one phase to its relative abundance in the other phase using two normalization steps.  First, an average for the total signal of the six arrays from each growth phase was calculated (1256 was the average in exponential phase and 389 was the average in stationary phase).  To correct for signal variations between replicate arrays, the value for each gene was adjusted by a factor so that the total signal for each array was equal to the average for the six in that growth phase. Second, the value for each gene adjusted for differences between replicates was divided by the average total signal in exponential phase. Normalized data was then log2 transformed, and statistical analysis and fold changes in expression in stationary phase versus exponential phase were obtained using Significance Analysis of Microarrays (SAM) software

molecule: total RNA

organism: Streptococcus pyogenes

source_name: Pooled Stationary phase CsCl2 total RNA from M3 serotype GAS MGAS315

tag: Strain

value: MGAS315  RNA prep:7-9 St

source_name: Pooled CsCl2 total RNA from M3 serotype GAS MGAS315

tag: Strains

value: MGAS315   RNA preps:1-9 Ex and 1-9 St


title: MGAS315 1-3 St -2

value: MGAS315  RNA prep:1-3 St


title: MGAS315 4-6 Ex -1

source_name: Pooled Exponential phase CsCl2 total RNA from M3 serotype GAS MGAS315

value: MGAS315  RNA prep:4-6 Ex


title: MGAS315 1-3 St -1


title: MGAS315 7-9 Ex -2

value: MGAS315  RNA prep:7-9 Ex


title: Sun215_TAV12 group

city: Rochester

first: Ramanath

last: Majumdar

state: MN

address: 200 1st St, SW

institute: Mayo Clinic

description: Gene expression data from aneurismal tissues obtained from patients with tricuspid valves.

hyb_protocol: Following  fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133A array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.

scan_protocol: GeneChips were scanned using Affymetrix GeneChip Scanner 3000 according to the manufacturer's protocol.

data_processing: Data analysis was performed using a linear model. The probe level data were normalized using Fastlo (Ballman et al. Bioinformatics 2004; 20:2778–2786) a modified version of cyclic loess normalization (Chu et al. Math Bio 2002;176: 35-51).

label: biotin

organism: Homo sapiens

source_name: aorta

label_protocol: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 microg total RNA (Expression analysis Technical Manual, 2001, Affymetrix).

extract_protocol: Total RNA from aortic tissue was extracted using Mini to Midi total RNA extraction kit (Invitrogen, Carlsbad, CA).

treatment_protocol: Tissue from ascending aortic aneurysms from patients undergoing ascending aortic surgery were collected. A portion of the tissue was snap frozen in liquid nitrogen and stored at –80oC until RNA extraction.

tag: aortic aneurysm


title: MGAS315 4-6 St -2

value: MGAS315  RNA prep:4-6 St


title: Sun212_TAV12 group


title: MGAS315 7-9 St -2


title: MGAS315 7-9 Ex -1


title: Sun199_TAV12 group


title: MGAS315 4-6 Ex -2


title: Sun197_TAV12 group


title: MGAS315 1-3 Ex -1

description: Total RNA was isolated by CsCl2 method and labeled cDNA was generated using the Fairplay microarray labelling kit from Stratagene using random hexamers.  Labeled cDNA was purified using QiaQuick PCR purification columns from Qiagen and concentrated using Microcon YM30 columns from Amicon.  The amount of incorporated label was determined by measuring the absorbance at 550nm (Cy3) or 650nm (Cy5). 60 pmol each of Cy-dye (reference cDNA (Cy5) and test cDNA (Cy3)) were combined in 60 ml hybridization buffer 1 (Ambion), denatured and hybridized to the array at 42°C for at least 16 h, followed by subsequent washes.  Output data (F635 mean-B635, F532 mean-B532, flags) was filtered to set any spots that did not exceed 2SD above a background threshold to the threshold value to avoid spurious expression ratios. Remaining spots were averaged, filtered to remove genes not present in MGAS315 (the strain examined) and normalized before statistical analysis.

value: MGAS315  RNA prep:1-3 Ex


title: Sun196_TAV12 group


title: MGAS315 1-3 Ex -2


title: Sun180_BAV13 group

description: Gene expression data from aneurismal tissues obtained from patients with bicuspid valves.


title: MGAS315 4-6 St -1


title: Sun205_TAV12 group


title: Sun188_BAV13 group


title: Sun181_BAV13 group


title: 2. undifferentiated 3D T84 culture

status: Public on Jun 27 2007

city: Tampere

first: Kati

middle: Marjaana

last: Juuti-Uusitalo

address: Biokatu 10

department: Medical School

country: Finland

institute: Pediatric Research Center

description: The normalisation was done in excell with sum method (Kroll et al. 2002)

hyb_protocol: Array was hybridized for 12 h at 42oC in roller bottle. After hybridization membrane was washed twice in 2 X SSC 1%SDS, 0.5XSSC.

scan_protocol: The hybridized membrane was let to expose Phosphoimager screens for 12 h. Hybridization signals were detected on a Storm 860 phosphoimager (Molecular Dynamics, Amersham Biosciences, Buckinghamshire, England)

data_processing: Filter images were aligned and spot intensities analysed with Pathways Software (Research Genetics). Raw data were imported to Microsoft Excel.

label: 33P dCTP (ICN Radiochemicals)

label_protocol: Probe preparation and microarray hybridization were performed following manufacturer's (Research Genetics) protocol using 1.5 ug total RNA as template, 10 ul (10mCi/ml) 33P dCTP (ICN Radiochemicals), 1,5 ul dNTP mix containing dATP, dTTP, dGTP at 20 mM (Pharmacia ), 1,5 ul reverse transcriptase (Supercript II, Life Technologies), 1,0 ul DTT. Elongation for 90 min at 37oC. The label was purified with Bio-Spin 6 Chromatography column (Bio-Rad),

growth_protocol: Human intestinal epithelial T84 cells (CCL 2´48, ATCC Rockville, MD, USA) were cultured in three-dimensional type I collagen gel as previously described Halttunen et al. 1996. T84 cells were induced to differentiate by adding 20 ng/ml human recombinant TGFbeta1 (hTGF-beta1, R&D Systems Europe, Oxon, UK). The cells were cultured 7 days in incubator.

extract_protocol: The mRNA was extracted from the cell culture samples to ice-cold TRIzol reagent (Life Technologies, Inc. Frederick, MD, USA) according to the manufacturers protocol. All samples were subjected to DNAse I treatment (Roche Diagnostics GmbH, Mannheim, Germany). Total RNA was quantitated by spectrophotometry and quality checked by agarose gel electrophoresis.

treatment_protocol: Human intestinal epithelial T84 cells (CCL 2´48, ATCC Rockville, MD, USA) were cultured in three-dimensional type I collagen gel as previously described Halttunen et al. 1996. T84 cells cultured within collagen gel supplemented with medium were used as undifferentiated control. The cells were cultured 7 days in incubator.

tag: Human intestinal epithelial T84 cells (CCL 2´48, ATCC Rockville, MD, USA)  cultured within collagen gel supplemented with medium form unorganised cuboidal clusters, and do not differentiate (Halttunen et al. 1996).


title: Sun211_TAV12 group


title: Sun177_BAV13 group


title: 1. IMR differentiated 3D T84 culture

extract_protocol: The mRNA was extracted from the cell culture samples (only from T84 cells)  to ice-cold TRIzol reagent (Life Technologies, Inc. Frederick, MD, USA) according to the manufacturer�s protocol. All samples were subjected to DNAse I treatment (Roche Diagnostics GmbH, Mannheim, Germany). Total RNA was quantitated by spectrophotometry and quality checked by agarose gel electrophoresis.

treatment_protocol: Human intestinal epithelial T84 cells (CCL 2´48, ATCC Rockville, MD, USA) were cultured in three-dimensional type I collagen gel as previously described Halttunen et al. 1996. T84 cells were induced to differentiate by soluble factors secreted by IMR-90 type human embryonic lung fibroblasts (CCL 186, ATCC). IMR fibroblasts, cultured on the top of the epithelial cells, were separated from the epithelial cells by cell-free collagen layer. The cells were cultured 7 days in incubator.

tag: T84 cells are induced to differentiate by the soluble factors secreted by the IMR fibroblasts. The epithelial crypt type cells are differentiated to enterocyte s.


title: Sun179_BAV13 group


title: Sun183_BAV13 group


title: Sun186_BAV13 group


title: Sun204_TAV12 group


title: Sun206_TAV12 group


title: Sun208_TAV12 group


title: Sun200_TAV12 group


title: 1. TGF beta1 differentiated 3D T84 culture

label_protocol: Probe preparation and microarray hybridization were performed following manufacturer's (Research Genetics) protocol using 1.5 µg total RNA as template, 10µl (10mCi/ml) 33P dCTP (ICN Radiochemicals), 1,5µl dNTP mix containing dATP, dTTP, dGTP at 20 mM (Pharmacia ), 1,5µl reverse transcriptase (Supercript II, Life Technologies), 1,0 µl DTT. Elongation for 90 min at 37oC. The label was purified with Bio-Spin 6 Chromatography column (Bio-Rad),

extract_protocol: The mRNA was extracted from the cell culture samples to ice-cold TRIzol reagent (Life Technologies, Inc. Frederick, MD, USA) according to the manufacturer's protocol. All samples were subjected to DNAse I treatment (Roche Diagnostics GmbH, Mannheim, Germany). Total RNA was quantitated by spectrophotometry and quality checked by agarose gel electrophoresis.

treatment_protocol: Human intestinal epithelial T84 cells (CCL 2´48, ATCC Rockville, MD, USA) were cultured in three-dimensional type I collagen gel as previously described Halttunen et al. 1996. T84 cells were induced to differentiate by adding 20 ng/ml human recombinant TGFbeta1 (hTGF-beta1, R&D Systems Europe, Oxon, UK). The cells were cultured 7 days in incubator.

tag: The T84 cells are induced to differentiate from crypt like cells to enterocytes.


title: offspring of cross of 89x83 female D. melanogaster, biological rep 2

status: Public on Jun 22 2007

city: Gainesville

first: Lauren

middle: M.

last: McIntyre

state: FL

department: Dept. of Molecular Genetics and Microbiology

institute: University of Florida

description: cross 89x83 female D. melanogaster

data_processing: Imagene v6.0 was used to process image and all results from Imag

organism: Drosophila melanogaster

source_name: cross 89x83 female D. melanogaster

tag: 20 pooled adult female individuals


title: offspring of cross of 89x75 male D. melanogaster, biological rep 2

description: cross 89x75 male D. melanogaster

source_name: cross 89x75 male D. melanogaster

tag: 20 pooled adult male individuals


title: offspring of cross of 89x83 male D. melanogaster, biological rep 2

description: cross 89x83 male D. melanogaster

source_name: cross 89x83 male D. melanogaster


title: offspring of cross of 89x58 female D. melanogaster, biological rep 2

description: cross 89x58 female D. melanogaster

source_name: cross 89x58 female D. melanogaster


title: Sun194_TAV12 group


title: offspring of cross of 89x23 female D. melanogaster, biological rep 2

description: cross 89x23 female D. melanogaster

source_name: cross 89x23 female D. melanogaster


title: offspring of cross of 89x23 female D. melanogaster, biological rep 1

description: cross 89x23 female D. melanogaster


title: Sun187_BAV13 group


title: offspring of cross of 89x75 female D. melanogaster, biological rep 2

description: cross 89x75 female D. melanogaster

source_name: cross 89x75 female D. melanogaster


title: Sun189_BAV13 group


title: offspring of cross of 89x17 male D. melanogaster, biological rep 1

description: cross 89x17 male D. melanogaster

source_name: cross 89x17 male D. melanogaster


title: Sun182_BAV13 group


title: offspring of cross of 89x145 male D. melanogaster, biological rep 1

description: cross 89x145 male D. melanogaster

source_name: cross 89x145 male D. melanogaster


title: 3. undifferentiated 3D T84 culture

source_name: Human intestinal epithelial T84 cells (CCL 2´48, ATCC Rockville, MD, USA)

tag: Human intestinal epithelial T84 cells cultured within collagen gel and supplemented with medium form unorganised cuboidal clusters and do not differentiate but remain as crypt type epithelial cells.


title: offspring of cross of 89x17 female D. melanogaster, biological rep 1

description: cross 89x17 female D. melanogaster

source_name: cross 89x17 female D. melanogaster


title: Sun185_BAV13 group


title: offspring of cross of 89x145 male D. melanogaster, biological rep 2

description: cross 89x145 male D. melanogaster


title: Sun176_BAV13 group


title: offspring of cross of 89x145 female D. melanogaster, biological rep 1

description: cross 89x145 female D. melanogaster

source_name: cross 89x145 female D. melanogaster


title: 1. undifferentiated 3D T84 culture

tag: Human intestinal epithelial T84 cells cultured within collagen gel and supplemented with medium form unorganised cuboidal clusters, and do not differentiate (Halttunen et al. 1996).


title: offspring of cross of 89x17 female D. melanogaster, biological rep 2

description: cross 89x17 female D. melanogaster


title: 2. IMR differentiated 3D T84 culture

tag: Human intestinal epithelial T84 cells are induced to differentiate from crypt like epithelial cells to enterocytes by the soluble factors secreted by the IMR fibroblasts.


title: 3. IMR differentiated 3D T84 culture

growth_protocol: Human intestinal epithelial T84 cells (CCL 2½48, ATCC Rockville, MD, USA) were cultured in three-dimensional type I collagen gel as previously described Halttunen et al. 1996. T84 cells were induced to differentiate by adding 20 ng/ml human recombinant TGFbeta1 (hTGF-beta1, R&D Systems Europe, Oxon, UK). The cells were cultured 7 days in incubator.

extract_protocol: The mRNA was extracted from the cell culture samples (only from T84 cells)  to ice-cold TRIzol reagent (Life Technologies, Inc. Frederick, MD, USA) according to the manufacturers protocol. All samples were subjected to DNAse I treatment (Roche Diagnostics GmbH, Mannheim, Germany). Total RNA was quantitated by spectrophotometry and quality checked by agarose gel electrophoresis.

tag: Human intestinal epithelial T84 cells are induced to differentiate from crypt type epithelial cells to enterocytes by the soluble factors secreted by the IMR fibroblasts.


title: offspring of cross of 89x134 female D. melanogaster, biological rep 2

description: cross 89x134 female D. melanogaster

source_name: cross 89x134 female D. melanogaster


title: offspring of cross of 89x128 male D. melanogaster, biological rep 2

description: cross 89x128 male D. melanogaster

source_name: cross 89x128 male D. melanogaster


title: 3. TGF beta1 differentiated 3D T84 culture

extract_protocol: The mRNA was extracted from the cell culture samples to ice-cold TRIzol reagent (Life Technologies, Inc. Frederick, MD, USA) according to the manufacturer�s protocol. All samples were subjected to DNAse I treatment (Roche Diagnostics GmbH, Mannheim, Germany). Total RNA was quantitated by spectrophotometry and quality checked by agarose gel electrophoresis.

tag: T84 cells are induced to differentiate from crypt tyope epithelial cells to enterocytes.


title: Sun178_BAV13 group


title: offspring of cross of 89x134 female D. melanogaster, biological rep 1

description: cross 89x134 female D. melanogaster


title: 2. TGF beta1 differentiated 3D T84 culture

label_protocol: Probe preparation and microarray hybridization were performed following manufacturer's (Research Genetics) protocol using 1.5 ug total RNA as template, 10 ul (10mCi/ml) 33P dCTP (ICN Radiochemicals), 1,5 ul dNTP mix containing dATP, dTTP, dGTP at 20 mM (Pharmacia ), 1,5 ul reverse transcriptase (Supercript II, Life Technologies), 1,0 DTT. Elongation for 90 min at 37oC. The label was purified with Bio-Spin 6 Chromatography column (Bio-Rad),

tag: T84 cells are induced to differentiate from crypt type epithelial cells to enterosytes


title: offspring of cross of 83x89 male D. melanogaster, biological rep 2

description: cross 83x89 male D. melanogaster

source_name: cross 83x89 male D. melanogaster


title: offspring of cross of 89x128 female D. melanogaster, biological rep 1

description: cross 89x128 female D. melanogaster

source_name: cross 89x128 female D. melanogaster


title: offspring of cross of 83x89 female D. melanogaster, biological rep 2

description: cross 83x89 female D. melanogaster

source_name: cross 83x89 female D. melanogaster


title: offspring of cross of 89x83 female D. melanogaster, biological rep 1

description: cross 89x83 female D. melanogaster


title: offspring of cross of 83x75 male D. melanogaster, biological rep 2

description: cross 83x75 male D. melanogaster

source_name: cross 83x75 male D. melanogaster


title: offspring of cross of 89x75 male D. melanogaster, biological rep 1

description: cross 89x75 male D. melanogaster


title: offspring of cross of 89x75 female D. melanogaster, biological rep 1

description: cross 89x75 female D. melanogaster


title: offspring of cross of 83x58 female D. melanogaster, biological rep 2

description: cross 83x58 female D. melanogaster

source_name: cross 83x58 female D. melanogaster


title: offspring of cross of 89x83 male D. melanogaster, biological rep 1

description: cross 89x83 male D. melanogaster


title: offspring of cross of 83x75 female D. melanogaster, biological rep 2

description: cross 83x75 female D. melanogaster

source_name: cross 83x75 female D. melanogaster


title: offspring of cross of 83x23 male D. melanogaster, biological rep 2

description: cross 83x23 male D. melanogaster

source_name: cross 83x23 male D. melanogaster


title: offspring of cross of 83x75 female D. melanogaster, biological rep 1

description: cross 83x75 female D. melanogaster


title: offspring of cross of 83x23 female D. melanogaster, biological rep 2

description: cross 83x23 female D. melanogaster

source_name: cross 83x23 female D. melanogaster


title: offspring of cross of 89x58 male D. melanogaster, biological rep 1

description: cross 89x58 male D. melanogaster

source_name: cross 89x58 male D. melanogaster


title: offspring of cross of 89x58 female D. melanogaster, biological rep 1

description: cross 89x58 female D. melanogaster

source_name: cross 89x58 female D. melanogaster


title: offspring of cross of 83x17 male D. melanogaster, biological rep 2

description: cross 83x17 male D. melanogaster

source_name: cross 83x17 male D. melanogaster


title: offspring of cross of 83x17 male D. melanogaster, biological rep 1

description: cross 83x17 male D. melanogaster


title: offspring of cross of 89x58 male D. melanogaster, biological rep 2

description: cross 89x58 male D. melanogaster


title: offspring of cross of 89x23 male D. melanogaster, biological rep 2

description: cross 89x23 male D. melanogaster

source_name: cross 89x23 male D. melanogaster


title: offspring of cross of 83x145 male D. melanogaster, biological rep 2

description: cross 83x145 male D. melanogaster

source_name: cross 83x145 male D. melanogaster


title: offspring of cross of 83x17 female D. melanogaster, biological rep 2

description: cross 83x17 female D. melanogaster

source_name: cross 83x17 female D. melanogaster


title: offspring of cross of 83x145 female D. melanogaster, biological rep 2

description: cross 83x145 female D. melanogaster

source_name: cross 83x145 female D. melanogaster


title: offspring of cross of 89x17 male D. melanogaster, biological rep 2

description: cross 89x17 male D. melanogaster

source_name: cross 89x17 male D. melanogaster


title: offspring of cross of 83x89 male D. melanogaster, biological rep 1

description: cross 83x89 male D. melanogaster


title: offspring of cross of 83x134 female D. melanogaster, biological rep 1

description: cross 83x134 female D. melanogaster

source_name: cross 83x134 female D. melanogaster


title: offspring of cross of 83x128 male D. melanogaster, biological rep 2

description: cross 83x128 male D. melanogaster

source_name: cross 83x128 male D. melanogaster


title: offspring of cross of 89x134 male D. melanogaster, biological rep 2

description: cross 89x134 male D. melanogaster

source_name: cross 89x134 male D. melanogaster


title: offspring of cross of 89x134 male D. melanogaster, biological rep 1

description: cross 89x134 male D. melanogaster


title: offspring of cross of 89x145 female D. melanogaster, biological rep 2

description: cross 89x145 female D. melanogaster

source_name: cross 89x145 female D. melanogaster


title: offspring of cross of 75x89 male D. melanogaster, biological rep 2

description: cross 75x89 male D. melanogaster

source_name: cross 75x89 male D. melanogaster


title: offspring of cross of 83x128 female D. melanogaster, biological rep 2

description: cross 83x128 female D. melanogaster

source_name: cross 83x128 female D. melanogaster


title: offspring of cross of 75x89 female D. melanogaster, biological rep 2

description: cross 75x89 female D. melanogaster

source_name: cross 75x89 female D. melanogaster


title: offspring of cross of 83x128 female D. melanogaster, biological rep 1

description: cross 83x128 female D. melanogaster


title: offspring of cross of 75x83 male D. melanogaster, biological rep 1

description: cross 75x83 male D. melanogaster

source_name: cross 75x83 male D. melanogaster


title: offspring of cross of 75x83 male D. melanogaster, biological rep 2

description: cross 75x83 male D. melanogaster


title: offspring of cross of 89x128 female D. melanogaster, biological rep 2

description: cross 89x128 female D. melanogaster

source_name: cross 89x128 female D. melanogaster


title: offspring of cross of 83x75 male D. melanogaster, biological rep 1

description: cross 83x75 male D. melanogaster

source_name: cross 83x75 male D. melanogaster


title: offspring of cross of 83x89 female D. melanogaster, biological rep 1

description: cross 83x89 female D. melanogaster

source_name: cross 83x89 female D. melanogaster


title: offspring of cross of 89x128 male D. melanogaster, biological rep 1

description: cross 89x128 male D. melanogaster

source_name: cross 89x128 male D. melanogaster


title: offspring of cross of 83x58 male D. melanogaster, biological rep 1

description: cross 83x58 male D. melanogaster

source_name: cross 83x58 male D. melanogaster


title: offspring of cross of 83x58 male D. melanogaster, biological rep 2

description: cross 83x58 male D. melanogaster


title: offspring of cross of 75x58 male D. melanogaster, biological rep 2

description: cross 75x58 male D. melanogaster

source_name: cross 75x58 male D. melanogaster


title: offspring of cross of 75x83 female D. melanogaster, biological rep 1

description: cross 75x83 female D. melanogaster

source_name: cross 75x83 female D. melanogaster


title: offspring of cross of 83x23 male D. melanogaster, biological rep 1

description: cross 83x23 male D. melanogaster

source_name: cross 83x23 male D. melanogaster


title: offspring of cross of 83x58 female D. melanogaster, biological rep 1

description: cross 83x58 female D. melanogaster

source_name: cross 83x58 female D. melanogaster


title: offspring of cross of 83x23 female D. melanogaster, biological rep 1

description: cross 83x23 female D. melanogaster

source_name: cross 83x23 female D. melanogaster


title: offspring of cross of 83x17 female D. melanogaster, biological rep 1

description: cross 83x17 female D. melanogaster

source_name: cross 83x17 female D. melanogaster


title: offspring of cross of 75x23 male D. melanogaster, biological rep 2

description: cross 75x23 male D. melanogaster

source_name: cross 75x23 male D. melanogaster


title: offspring of cross of 75x17 male D. melanogaster, biological rep 1

description: cross 75x17 male D. melanogaster

source_name: cross 75x17 male D. melanogaster


title: offspring of cross of 75x17 male D. melanogaster, biological rep 2

description: cross 75x17 male D. melanogaster


title: offspring of cross of 89x23 male D. melanogaster, biological rep 1

description: cross 89x23 male D. melanogaster

source_name: cross 89x23 male D. melanogaster


title: offspring of cross of 75x23 female D. melanogaster, biological rep 1

description: cross 75x23 female D. melanogaster

source_name: cross 75x23 female D. melanogaster


title: offspring of cross of 83x145 male D. melanogaster, biological rep 1

description: cross 83x145 male D. melanogaster

source_name: cross 83x145 male D. melanogaster


title: offspring of cross of 75x145 male D. melanogaster, biological rep 2

description: cross 75x145 male D. melanogaster

source_name: cross 75x145 male D. melanogaster


title: offspring of cross of 83x134 male D. melanogaster, biological rep 2

description: cross 83x134 male D. melanogaster

source_name: cross 83x134 male D. melanogaster


title: offspring of cross of 75x17 female D. melanogaster, biological rep 2

description: cross 75x17 female D. melanogaster

source_name: cross 75x17 female D. melanogaster


title: offspring of cross of 83x134 male D. melanogaster, biological rep 1

description: cross 83x134 male D. melanogaster


title: offspring of cross of 83x145 female D. melanogaster, biological rep 1

description: cross 83x145 female D. melanogaster

source_name: cross 83x145 female D. melanogaster


title: offspring of cross of 83x134 female D. melanogaster, biological rep 2

description: cross 83x134 female D. melanogaster

source_name: cross 83x134 female D. melanogaster


title: offspring of cross of 83x128 male D. melanogaster, biological rep 1

description: cross 83x128 male D. melanogaster

source_name: cross 83x128 male D. melanogaster


title: offspring of cross of 75x145 female D. melanogaster, biological rep 2

description: cross 75x145 female D. melanogaster

source_name: cross 75x145 female D. melanogaster


title: offspring of cross of 75x89 male D. melanogaster, biological rep 1

description: cross 75x89 male D. melanogaster

source_name: cross 75x89 male D. melanogaster


title: offspring of cross of 75x89 female D. melanogaster, biological rep 1

description: cross 75x89 female D. melanogaster

source_name: cross 75x89 female D. melanogaster


title: offspring of cross of 75x128 male D. melanogaster, biological rep 2

description: cross 75x128 male D. melanogaster

source_name: cross 75x128 male D. melanogaster


title: offspring of cross of 75x134 female D. melanogaster, biological rep 1

description: cross 75x134 female D. melanogaster

source_name: cross 75x134 female D. melanogaster


title: offspring of cross of 75x128 female D. melanogaster, biological rep 1

description: cross 75x128 female D. melanogaster

source_name: cross 75x128 female D. melanogaster


title: offspring of cross of 75x83 female D. melanogaster, biological rep 2

description: cross 75x83 female D. melanogaster

source_name: cross 75x83 female D. melanogaster


title: offspring of cross of 58x83 female D. melanogaster, biological rep 2

description: cross 58x83 female D. melanogaster

source_name: cross 58x83 female D. melanogaster


title: offspring of cross of 58x83 female D. melanogaster, biological rep 1

description: cross 58x83 female D. melanogaster


title: offspring of cross of 75x58 female D. melanogaster, biological rep 2

description: cross 75x58 female D. melanogaster

source_name: cross 75x58 female D. melanogaster


title: offspring of cross of 75x58 female D. melanogaster, biological rep 1

description: cross 75x58 female D. melanogaster


title: offspring of cross of 58x83 male D. melanogaster, biological rep 1

description: cross 58x83 male D. melanogaster

source_name: cross 58x83 male D. melanogaster


title: offspring of cross of 58x89 female D. melanogaster, biological rep 1

description: cross 58x89 female D. melanogaster

source_name: cross 58x89 female D. melanogaster


title: offspring of cross of 75x58 male D. melanogaster, biological rep 1

description: cross 75x58 male D. melanogaster

source_name: cross 75x58 male D. melanogaster


title: offspring of cross of 75x23 male D. melanogaster, biological rep 1

description: cross 75x23 male D. melanogaster

source_name: cross 75x23 male D. melanogaster


title: offspring of cross of 75x23 female D. melanogaster, biological rep 2

description: cross 75x23 female D. melanogaster

source_name: cross 75x23 female D. melanogaster


title: offspring of cross of 58x75 female D. melanogaster, biological rep 2

description: cross 58x75 female D. melanogaster

source_name: cross 58x75 female D. melanogaster


title: offspring of cross of 75x17 female D. melanogaster, biological rep 1

description: cross 75x17 female D. melanogaster

source_name: cross 75x17 female D. melanogaster


title: offspring of cross of 75x145 male D. melanogaster, biological rep 1

description: cross 75x145 male D. melanogaster

source_name: cross 75x145 male D. melanogaster


title: offspring of cross of 58x17 male D. melanogaster, biological rep 1

description: cross 58x17 male D. melanogaster

source_name: cross 58x17 male D. melanogaster


title: offspring of cross of 58x145 male D. melanogaster, biological rep 2

description: cross 58x145 male D. melanogaster

source_name: cross 58x145 male D. melanogaster


title: offspring of cross of 75x145 female D. melanogaster, biological rep 1

description: cross 75x145 female D. melanogaster

source_name: cross 75x145 female D. melanogaster


title: offspring of cross of 58x145 female D. melanogaster, biological rep 2

description: cross 58x145 female D. melanogaster

source_name: cross 58x145 female D. melanogaster


title: offspring of cross of 75x134 male D. melanogaster, biological rep 2

description: cross 75x134 male D. melanogaster

source_name: cross 75x134 male D. melanogaster


title: offspring of cross of 75x134 male D. melanogaster, biological rep 1

description: cross 75x134 male D. melanogaster


title: offspring of cross of 58x134 male D. melanogaster, biological rep 1

description: cross 58x134 male D. melanogaster

source_name: cross 58x134 male D. melanogaster


title: offspring of cross of 58x145 female D. melanogaster, biological rep 1

description: cross 58x145 female D. melanogaster


title: offspring of cross of 75x134 female D. melanogaster, biological rep 2

description: cross 75x134 female D. melanogaster

source_name: cross 75x134 female D. melanogaster


title: offspring of cross of 58x134 male D. melanogaster, biological rep 2

description: cross 58x134 male D. melanogaster


title: offspring of cross of 58x17 female D. melanogaster, biological rep 2

description: cross 58x17 female D. melanogaster

source_name: cross 58x17 female D. melanogaster


title: offspring of cross of 58x134 female D. melanogaster, biological rep 2

description: cross 58x134 female D. melanogaster

source_name: cross 58x134 female D. melanogaster


title: offspring of cross of 75x128 female D. melanogaster, biological rep 2

description: cross 75x128 female D. melanogaster

source_name: cross 75x128 female D. melanogaster


title: offspring of cross of 58x128 male D. melanogaster, biological rep 1

description: cross 58x128 male D. melanogaster

source_name: cross 58x128 male D. melanogaster


title: offspring of cross of 58x89 male D. melanogaster, biological rep 2

description: cross 58x89 male D. melanogaster

source_name: cross 58x89 male D. melanogaster


title: offspring of cross of 58x89 male D. melanogaster, biological rep 1

description: cross 58x89 male D. melanogaster


title: offspring of cross of 75x128 male D. melanogaster, biological rep 1

description: cross 75x128 male D. melanogaster

source_name: cross 75x128 male D. melanogaster


title: offspring of cross of 58x128 male D. melanogaster, biological rep 2

description: cross 58x128 male D. melanogaster


title: offspring of cross of 23x89 female D. melanogaster, biological rep 2

description: cross 23x89 female D. melanogaster

source_name: cross 23x89 female D. melanogaster


title: offspring of cross of 23x89 male D. melanogaster, biological rep 2

description: cross 23x89 male D. melanogaster

source_name: cross 23x89 male D. melanogaster


title: offspring of cross of 23x83 female D. melanogaster, biological rep 2

description: cross 23x83 female D. melanogaster

source_name: cross 23x83 female D. melanogaster


title: offspring of cross of 58x83 male D. melanogaster, biological rep 2

description: cross 58x83 male D. melanogaster

source_name: cross 58x83 male D. melanogaster


title: offspring of cross of 58x89 female D. melanogaster, biological rep 2

description: cross 58x89 female D. melanogaster

source_name: cross 58x89 female D. melanogaster


title: offspring of cross of 23x89 female D. melanogaster, biological rep 1

description: cross 23x89 female D. melanogaster


title: offspring of cross of 23x89 male D. melanogaster, biological rep 1

description: cross 23x89 male D. melanogaster


title: offspring of cross of 58x128 female D. melanogaster, biological rep 1

description: cross 58x128 female D. melanogaster

source_name: cross 58x128 female D. melanogaster


title: offspring of cross of 23x83 male D. melanogaster, biological rep 2

description: cross 23x83 male D. melanogaster

source_name: cross 23x83 male D. melanogaster


title: offspring of cross of 58x75 male D. melanogaster, biological rep 2

description: cross 58x75 male D. melanogaster

source_name: cross 58x75 male D. melanogaster


title: offspring of cross of 58x75 male D. melanogaster, biological rep 1

description: cross 58x75 male D. melanogaster


title: offspring of cross of 58x23 male D. melanogaster, biological rep 2

description: cross 58x23 male D. melanogaster

source_name: cross 58x23 male D. melanogaster


title: offspring of cross of 23x75 female D. melanogaster, biological rep 2

description: cross 23x75 female D. melanogaster

source_name: cross 23x75 female D. melanogaster


title: offspring of cross of 58x75 female D. melanogaster, biological rep 1

description: cross 58x75 female D. melanogaster

source_name: cross 58x75 female D. melanogaster


title: offspring of cross of 23x58 male D. melanogaster, biological rep 1

description: cross 23x58 male D. melanogaster

source_name: cross 23x58 male D. melanogaster


title: offspring of cross of 23x75 female D. melanogaster, biological rep 1

description: cross 23x75 female D. melanogaster


title: offspring of cross of 23x17 female D. melanogaster, biological rep 2

description: cross 23x17 female D. melanogaster

source_name: cross 23x17 female D. melanogaster


title: offspring of cross of 23x17 male D. melanogaster, biological rep 1

description: cross 23x17 male D. melanogaster

source_name: cross 23x17 male D. melanogaster


title: offspring of cross of 58x23 female D. melanogaster, biological rep 2

description: cross 58x23 female D. melanogaster

source_name: cross 58x23 female D. melanogaster


title: offspring of cross of 23x145 female D. melanogaster, biological rep 2

description: cross 23x145 female D. melanogaster

source_name: cross 23x145 female D. melanogaster


title: offspring of cross of 58x17 male D. melanogaster, biological rep 2

description: cross 58x17 male D. melanogaster

source_name: cross 58x17 male D. melanogaster


title: offspring of cross of 23x145 female D. melanogaster, biological rep 1

description: cross 23x145 female D. melanogaster


title: offspring of cross of 58x23 female D. melanogaster, biological rep 1

description: cross 58x23 female D. melanogaster


title: offspring of cross of 23x145 male D. melanogaster, biological rep 1

description: cross 23x145 male D. melanogaster

source_name: cross 23x145 male D. melanogaster


title: offspring of cross of 58x23 male D. melanogaster, biological rep 1

description: cross 58x23 male D. melanogaster


title: offspring of cross of 58x17 female D. melanogaster, biological rep 1

description: cross 58x17 female D. melanogaster

source_name: cross 58x17 female D. melanogaster


title: offspring of cross of 58x145 male D. melanogaster, biological rep 1

description: cross 58x145 male D. melanogaster

source_name: cross 58x145 male D. melanogaster


title: offspring of cross of 23x17 female D. melanogaster, biological rep 1

description: cross 23x17 female D. melanogaster


title: offspring of cross of 23x134 male D. melanogaster, biological rep 2

description: cross 23x134 male D. melanogaster

source_name: cross 23x134 male D. melanogaster


title: offspring of cross of 23x128 male D. melanogaster, biological rep 2

description: cross 23x128 male D. melanogaster

source_name: cross 23x128 male D. melanogaster


title: offspring of cross of 17x89 male D. melanogaster, biological rep 2

description: cross 17x89 male D. melanogaster

source_name: cross 17x89 male D. melanogaster


title: offspring of cross of 17x89 female D. melanogaster, biological rep 2

description: cross 17x89 female D. melanogaster

source_name: cross 17x89 female D. melanogaster


title: offspring of cross of 23x128 female D. melanogaster, biological rep 1

description: cross 23x128 female D. melanogaster

source_name: cross 23x128 female D. melanogaster


title: offspring of cross of 17x89 female D. melanogaster, biological rep 1

description: cross 17x89 female D. melanogaster


title: offspring of cross of 23x83 male D. melanogaster, biological rep 1

description: cross 23x83 male D. melanogaster

source_name: cross 23x83 male D. melanogaster


title: offspring of cross of 58x128 female D. melanogaster, biological rep 2

description: cross 58x128 female D. melanogaster

source_name: cross 58x128 female D. melanogaster


title: offspring of cross of 58x134 female D. melanogaster, biological rep 1

description: cross 58x134 female D. melanogaster

source_name: cross 58x134 female D. melanogaster


title: offspring of cross of 17x83 male D. melanogaster, biological rep 2

description: cross 17x83 male D. melanogaster

source_name: cross 17x83 male D. melanogaster


title: offspring of cross of 23x83 female D. melanogaster, biological rep 1

description: cross 23x83 female D. melanogaster

source_name: cross 23x83 female D. melanogaster


title: offspring of cross of 17x75 female D. melanogaster, biological rep 1

description: cross 17x75 female D. melanogaster

source_name: cross 17x75 female D. melanogaster


title: offspring of cross of 17x58 male D. melanogaster, biological rep 2

description: cross 17x58 male D. melanogaster

source_name: cross 17x58 male D. melanogaster


title: offspring of cross of 17x75 female D. melanogaster, biological rep 2

description: cross 17x75 female D. melanogaster


title: offspring of cross of 23x75 male D. melanogaster, biological rep 2

description: cross 23x75 male D. melanogaster

source_name: cross 23x75 male D. melanogaster


title: offspring of cross of 23x75 male D. melanogaster, biological rep 1

description: cross 23x75 male D. melanogaster


title: offspring of cross of 23x58 male D. melanogaster, biological rep 2

description: cross 23x58 male D. melanogaster

source_name: cross 23x58 male D. melanogaster


title: offspring of cross of 17x23 female D. melanogaster, biological rep 1

description: cross 17x23 female D. melanogaster

source_name: cross 17x23 female D. melanogaster


title: offspring of cross of 23x17 male D. melanogaster, biological rep 2

description: cross 23x17 male D. melanogaster

source_name: cross 23x17 male D. melanogaster


title: offspring of cross of 23x58 female D. melanogaster, biological rep 2

description: cross 23x58 female D. melanogaster

source_name: cross 23x58 female D. melanogaster


title: offspring of cross of 17x134 male D. melanogaster, biological rep 1

description: cross 17x134 male D. melanogaster

source_name: cross 17x134 male D. melanogaster


title: offspring of cross of 17x145 female D. melanogaster, biological rep 1

description: cross 17x145 female D. melanogaster

source_name: cross 17x145 female D. melanogaster


title: offspring of cross of 17x145 female D. melanogaster, biological rep 2

description: cross 17x145 female D. melanogaster


title: offspring of cross of 23x145 male D. melanogaster, biological rep 2

description: cross 23x145 male D. melanogaster

source_name: cross 23x145 male D. melanogaster


title: offspring of cross of 23x134 female D. melanogaster, biological rep 2

description: cross 23x134 female D. melanogaster

source_name: cross 23x134 female D. melanogaster


title: offspring of cross of 23x134 male D. melanogaster, biological rep 1

description: cross 23x134 male D. melanogaster

source_name: cross 23x134 male D. melanogaster


title: offspring of cross of 17x128 male D. melanogaster, biological rep 1

description: cross 17x128 male D. melanogaster

source_name: cross 17x128 male D. melanogaster


title: offspring of cross of 17x134 female D. melanogaster, biological rep 1

description: cross 17x134 female D. melanogaster

source_name: cross 17x134 female D. melanogaster


title: offspring of cross of 23x128 male D. melanogaster, biological rep 1

description: cross 23x128 male D. melanogaster

source_name: cross 23x128 male D. melanogaster


title: offspring of cross of 23x128 female D. melanogaster, biological rep 2

description: cross 23x128 female D. melanogaster

source_name: cross 23x128 female D. melanogaster


title: offspring of cross of 17x89 male D. melanogaster, biological rep 1

description: cross 17x89 male D. melanogaster

source_name: cross 17x89 male D. melanogaster


title: offspring of cross of 17x128 female D. melanogaster, biological rep 1

description: cross 17x128 female D. melanogaster

source_name: cross 17x128 female D. melanogaster


title: offspring of cross of 23x134 female D. melanogaster, biological rep 1

description: cross 23x134 female D. melanogaster


title: offspring of cross of 145x89 male D. melanogaster, biological rep 1

description: cross 145x89 male D. melanogaster

source_name: cross 145x89 male D. melanogaster


title: offspring of cross of 17x83 male D. melanogaster, biological rep 1

description: cross 17x83 male D. melanogaster

source_name: cross 17x83 male D. melanogaster


title: offspring of cross of 17x23 male D. melanogaster, biological rep 2

description: cross 17x23 male D. melanogaster

source_name: cross 17x23 male D. melanogaster


title: offspring of cross of 17x83 female D. melanogaster, biological rep 2

description: cross 17x83 female D. melanogaster

source_name: cross 17x83 female D. melanogaster


title: offspring of cross of 17x83 female D. melanogaster, biological rep 1

description: cross 17x83 female D. melanogaster


title: offspring of cross of 17x75 male D. melanogaster, biological rep 2

description: cross 17x75 male D. melanogaster

source_name: cross 17x75 male D. melanogaster


title: offspring of cross of 17x75 male D. melanogaster, biological rep 1

description: cross 17x75 male D. melanogaster


title: offspring of cross of 17x58 male D. melanogaster, biological rep 1

description: cross 17x58 male D. melanogaster

source_name: cross 17x58 male D. melanogaster


title: offspring of cross of 17x58 female D. melanogaster, biological rep 2

description: cross 17x58 female D. melanogaster

source_name: cross 17x58 female D. melanogaster


title: offspring of cross of 17x23 female D. melanogaster, biological rep 2

description: cross 17x23 female D. melanogaster

source_name: cross 17x23 female D. melanogaster


title: offspring of cross of 145x75 male D. melanogaster, biological rep 2

description: cross 145x75 male D. melanogaster

source_name: cross 145x75 male D. melanogaster


title: offspring of cross of 145x83 female D. melanogaster, biological rep 1

description: cross 145x83 female D. melanogaster

source_name: cross 145x83 female D. melanogaster


title: offspring of cross of 17x23 male D. melanogaster, biological rep 1

description: cross 17x23 male D. melanogaster


title: offspring of cross of 17x145 male D. melanogaster, biological rep 2

description: cross 17x145 male D. melanogaster

source_name: cross 17x145 male D. melanogaster


title: offspring of cross of 23x58 female D. melanogaster, biological rep 1

description: cross 23x58 female D. melanogaster

source_name: cross 23x58 female D. melanogaster


title: offspring of cross of 17x58 female D. melanogaster, biological rep 1

description: cross 17x58 female D. melanogaster


title: offspring of cross of 17x134 male D. melanogaster, biological rep 2

description: cross 17x134 male D. melanogaster

source_name: cross 17x134 male D. melanogaster


title: offspring of cross of 145x89 female D. melanogaster, biological rep 2

description: cross 145x89 female D. melanogaster

source_name: cross 145x89 female D. melanogaster


title: offspring of cross of 17x128 male D. melanogaster, biological rep 2

description: cross 17x128 male D. melanogaster

source_name: cross 17x128 male D. melanogaster


title: offspring of cross of 17x134 female D. melanogaster, biological rep 2

description: cross 17x134 female D. melanogaster

source_name: cross 17x134 female D. melanogaster


title: offspring of cross of 17x128 female D. melanogaster, biological rep 2

description: cross 17x128 female D. melanogaster

source_name: cross 17x128 female D. melanogaster


title: offspring of cross of 145x58 male D. melanogaster, biological rep 2

description: cross 145x58 male D. melanogaster

source_name: cross 145x58 male D. melanogaster


title: offspring of cross of 145x89 female D. melanogaster, biological rep 1

description: cross 145x89 female D. melanogaster


title: offspring of cross of 145x134 male D. melanogaster, biological rep 2

description: cross 145x134 male D. melanogaster

source_name: cross 145x134 male D. melanogaster


title: offspring of cross of 145x75 female D. melanogaster, biological rep 2

description: cross 145x75 female D. melanogaster

source_name: cross 145x75 female D. melanogaster


title: offspring of cross of 145x58 male D. melanogaster, biological rep 1

description: cross 145x58 male D. melanogaster


title: offspring of cross of 145x128 female D. melanogaster, biological rep 2

description: cross 145x128 female D. melanogaster

source_name: cross 145x128 female D. melanogaster


title: offspring of cross of 145x23 female D. melanogaster, biological rep 1

description: cross 145x23 female D. melanogaster

source_name: cross 145x23 female D. melanogaster


title: offspring of cross of 145x17 female D. melanogaster, biological rep 1

description: cross 145x17 female D. melanogaster

source_name: cross 145x17 female D. melanogaster


title: offspring of cross of 145x58 female D. melanogaster, biological rep 2

description: cross 145x58 female D. melanogaster

source_name: cross 145x58 female D. melanogaster


title: offspring of cross of 145x23 female D. melanogaster, biological rep 2

description: cross 145x23 female D. melanogaster


title: offspring of cross of 145x75 male D. melanogaster, biological rep 1

description: cross 145x75 male D. melanogaster


title: offspring of cross of 145x134 male D. melanogaster, biological rep 1

description: cross 145x134 male D. melanogaster


title: offspring of cross of 145x128 male D. melanogaster, biological rep 1

description: cross 145x128 male D. melanogaster

source_name: cross 145x128 male D. melanogaster


title: offspring of cross of 134x89 female D. melanogaster, biological rep 2

description: cross 134x89 female D. melanogaster

source_name: cross 134x89 female D. melanogaster


title: offspring of cross of 134x89 male D. melanogaster, biological rep 2

description: cross 134x89 male D. melanogaster

source_name: cross 134x89 male D. melanogaster


title: offspring of cross of 145x17 male D. melanogaster, biological rep 1

description: cross 145x17 male D. melanogaster

source_name: cross 145x17 male D. melanogaster


title: offspring of cross of 145x58 female D. melanogaster, biological rep 1

description: cross 145x58 female D. melanogaster


title: offspring of cross of 145x134 female D. melanogaster, biological rep 2

description: cross 145x134 female D. melanogaster

source_name: cross 145x134 female D. melanogaster


title: offspring of cross of 145x83 male D. melanogaster, biological rep 2

description: cross 145x83 male D. melanogaster

source_name: cross 145x83 male D. melanogaster


title: offspring of cross of 145x128 female D. melanogaster, biological rep 1

description: cross 145x128 female D. melanogaster


title: offspring of cross of 145x17 male D. melanogaster, biological rep 2

description: cross 145x17 male D. melanogaster


title: offspring of cross of 134x89 female D. melanogaster, biological rep 1

description: cross 134x89 female D. melanogaster


title: offspring of cross of 134x83 male D. melanogaster, biological rep 2

description: cross 134x83 male D. melanogaster

source_name: cross 134x83 male D. melanogaster


title: offspring of cross of 134x83 female D. melanogaster, biological rep 1

description: cross 134x83 female D. melanogaster

source_name: cross 134x83 female D. melanogaster


title: offspring of cross of 145x23 male D. melanogaster, biological rep 1

description: cross 145x23 male D. melanogaster

source_name: cross 145x23 male D. melanogaster


title: offspring of cross of 145x128 male D. melanogaster, biological rep 2

description: cross 145x128 male D. melanogaster


title: offspring of cross of 134x75 male D. melanogaster, biological rep 2

description: cross 134x75 male D. melanogaster

source_name: cross 134x75 male D. melanogaster


title: offspring of cross of 145x83 female D. melanogaster, biological rep 2

description: cross 145x83 female D. melanogaster

source_name: cross 145x83 female D. melanogaster


title: offspring of cross of 145x17 female D. melanogaster, biological rep 2

description: cross 145x17 female D. melanogaster


title: offspring of cross of 145x23 male D. melanogaster, biological rep 2

description: cross 145x23 male D. melanogaster


title: offspring of cross of 145x75 female D. melanogaster, biological rep 1

description: cross 145x75 female D. melanogaster


title: offspring of cross of 145x83 male D. melanogaster, biological rep 1

description: cross 145x83 male D. melanogaster


title: offspring of cross of 134x89 male D. melanogaster, biological rep 1

description: cross 134x89 male D. melanogaster


title: offspring of cross of 17x145 male D. melanogaster, biological rep 1

description: cross 17x145 male D. melanogaster

source_name: cross 17x145 male D. melanogaster


title: offspring of cross of 145x134 female D. melanogaster, biological rep 1

description: cross 145x134 female D. melanogaster


title: offspring of cross of 145x89 male D. melanogaster, biological rep 2

description: cross 145x89 male D. melanogaster

source_name: cross 145x89 male D. melanogaster


title: offspring of cross of 134x75 male D. melanogaster, biological rep 1

description: cross 134x75 male D. melanogaster


title: offspring of cross of 134x83 female D. melanogaster, biological rep 2

description: cross 134x83 female D. melanogaster


title: Pooled sample of 40 adult D. melanogaster individuals, biological rep2

description: adult D. melanogaster

source_name: adult D. melanogaster

tag: 40 adult pooled individuals


title: Iris pigment epithelium

city: Tokyo

first: Yuri

last: Futagami

address: 1-5-45 Yushima Bunkyoku

department: Ophthalmology

country: Japan

institute: Tokyo Medical and Dental University

description: The primary IPE cultures were found to be greater than 99% cytokeratin positive (Clone PCK-26, Sigma) as determined by flow cytometry.

hyb_protocol: 10 migro grams of fragmented cRNA were hybridised (45 degrees Celsius, 16 hours). Hybridization was controled adding cRNA cocktail (BioC, BioD, Cre) mixed as described in previous versions of the Expression  Analysis Technical Manual. Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v5.

scan_protocol: Scanning was performed in an Affymetrix GeneChip scanner 3000.

data_processing: The data analysis were performed using the Affymetrix GeneChip Operating Software v1.3. using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of array was arbitrarily set to 100.

organism: Mus musculus

source_name: Primary cultured cells of iris pigment epithelium

label_protocol: 1 micro gram of total RNA was reverse transcribed using the cDNA synthesis system of MessageAmp aRNA Kit (Ambion). The oligonucleotide used for priming was 5'-ggccagtgaattgtaatacgactcactatagggaggcgg-(t)24-3. Double-stranded cDNA was cleaned by phenol:chloroform extraction and the aqueous phase removed by centrifugation through Phase-lock Gel (Eppendorf). In vitro transcription was performed using the 3'-Amplification Reagents for IVT Labeling (Affymetrix). The cRNA was cleaned using RNeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.

growth_protocol: Primary cultures of pigmented epithelial cells from the iris (IPE) were cultured in RPMI 1640 complete medium containing 10% fetal bovine serum (FBS). The medium of ocular PE was changed to serum free medium, with a cellular RNA extraction then performed after an overnight culture.

extract_protocol: Total RNA was isolated using TRIZOL Reagent(Invitrogen) and used as starting material for the cDNA preparation. RNA was purified from total cellular RNA by Nucleospin RNA II (Macherey-Nagel, Inc, Germany) as outlined by the manufacturer. Integrity of total RNA was assessed qualitatively on the Agilent 2100 Bioanalizer (Agilent Technologies).

treatment_protocol: The iris tissue was triturated to make a single cell suspension, and then re-suspended in the medium.

tag: Iris pigment epithelium (Primary cultured cell)


title: offspring of cross of 134x83 male D. melanogaster, biological rep 1

description: cross 134x83 male D. melanogaster


title: offspring of cross of 134x75 female D. melanogaster, biological rep 2

description: cross 134x75 female D. melanogaster

source_name: cross 134x75 female D. melanogaster


title: Pooled sample of 20 adult D. melanogaster individuals, biological rep2

description: adult D. melanogaster

tag: 20 adult pooled individuals


title: offspring of cross of 134x75 female D. melanogaster, biological rep 1

description: cross 134x75 female D. melanogaster


title: Ciliary body pigment epithelium

description: The primary CBPE cultures were found to be greater than 95% cytokeratin positive after 14 days of incubation.

source_name: Primary cultured cells of ciliary body pigment epithelium

growth_protocol: Primary cultures of pigmented epithelial cells from the ciliary body (CBPE) were cultured in RPMI 1640 complete medium containing 10% fetal bovine serum (FBS). The medium of ocular PE was changed to serum free medium, with a cellular RNA extraction then performed after an overnight culture.

treatment_protocol: The ciliary body tissue was triturated to make a single cell suspension, and then re-suspended in the medium.

tag: Ciliary body pigment epithelium (Primary cultured cell)


title: Retinal pigment epithelium

description: The primary RPE cultures were found to be greater than 95% cytokeratin positive after 14 days of incubation.

source_name: Primary cultured cells of retinal pigment epithelium

growth_protocol: Primary cultures of pigmented epithelial cells from the retinal PE (RPE) were cultured in DMEM complete medium composed of DMEM and 20% FBS. The medium of ocular PE was changed to serum free medium, with a cellular RNA extraction then performed after an overnight culture.

treatment_protocol: The retinal tissue was triturated to make a single cell suspension, and then re-suspended in the medium.

tag: Retinal pigment epithelium (Primary cultured cell)


title: offspring of cross of 134x58 female D. melanogaster, biological rep 2

description: cross 134x58 female D. melanogaster

source_name: cross 134x58 female D. melanogaster


title: offspring of cross of 134x58 male D. melanogaster, biological rep 1

description: cross 134x58 male D. melanogaster

source_name: cross 134x58 male D. melanogaster


title: Pooled sample of 40 adult D. melanogaster individuals, biological rep1

description: adult D. melanogaster


title: offspring of cross of 134x58 male D. melanogaster, biological rep 2

description: cross 134x58 male D. melanogaster


title: Pooled sample of 10 adult D. melanogaster individuals, biological rep1

description: adult D. melanogaster

tag: 10 adult pooled individuals


title: Pooled sample of 10 adult D. melanogaster individuals, biological rep2

description: adult D. melanogaster


title: offspring of cross of 134x17 male D. melanogaster, biological rep 2

description: cross 134x17 male D. melanogaster

source_name: cross 134x17 male D. melanogaster


title: offspring of cross of 134x23 female D. melanogaster, biological rep 1

description: cross 134x23 female D. melanogaster

source_name: cross 134x23 female D. melanogaster


title: offspring of cross of 134x17 male D. melanogaster, biological rep 1

description: cross 134x17 male D. melanogaster


title: offspring of cross of 134x23 female D. melanogaster, biological rep 2

description: cross 134x23 female D. melanogaster


title: offspring of cross of 134x23 male D. melanogaster, biological rep 2

description: cross 134x23 male D. melanogaster

source_name: cross 134x23 male D. melanogaster


title: offspring of cross of 134x58 female D. melanogaster, biological rep 1

description: cross 134x58 female D. melanogaster


title: offspring of cross of 134x145 female D. melanogaster, biological rep 1

description: cross 134x145 female D. melanogaster

source_name: cross 134x145 female D. melanogaster


title: offspring of cross of 134x145 male D. melanogaster, biological rep 2

description: cross 134x145 male D. melanogaster

source_name: cross 134x145 male D. melanogaster


title: offspring of cross of 134x17 female D. melanogaster, biological rep 1

description: cross 134x17 female D. melanogaster

source_name: cross 134x17 female D. melanogaster


title: offspring of cross of 134x17 female D. melanogaster, biological rep 2

description: cross 134x17 female D. melanogaster


title: offspring of cross of 134x145 male D. melanogaster, biological rep 1

description: cross 134x145 male D. melanogaster


title: offspring of cross of 134x128 male D. melanogaster, biological rep 1

description: cross 134x128 male D. melanogaster

source_name: cross 134x128 male D. melanogaster


title: offspring of cross of 134x23 male D. melanogaster, biological rep 1

description: cross 134x23 male D. melanogaster


title: offspring of cross of 134x128 male D. melanogaster, biological rep 2

description: cross 134x128 male D. melanogaster


title: offspring of cross of 134x145 female D. melanogaster, biological rep 2

description: cross 134x145 female D. melanogaster


title: proboscis of male D. melanogaster rep2

description: RIL 89 male D. melanogaster

data_processing: Imagene v6.0 was used to process image and all results from Imagene listed

source_name: proboscis of male D. melanogaster

tag: 5 pooled adult male individuals


title: Pooled sample of 20 adult D. melanogaster individuals, biological rep1

description: adult D. melanogaster


title: first legs of female D. melanogaster rep3

description: RIL 89 female D. melanogaster

source_name: first legs of female D. melanogaster

tag: 8 pooled adult female individuals


title: proboscis of male D. melanogaster rep4

description: RIL 58 male D. melanogaster


title: offspring of cross of 134x128 female D. melanogaster, biological rep 1

description: cross 134x128 female D. melanogaster

source_name: cross 134x128 female D. melanogaster


title: proboscis of female D. melanogaster rep1

description: RIL 58 female D. melanogaster

source_name: proboscis of female D. melanogaster

tag: 10 pooled adult female individuals


title: antenna of male D. melanogaster rep2

description: RIL 58 male D. melanogaster

source_name: antenna of male D. melanogaster

tag: 10 pooled adult male individuals


title: first legs of male D. melanogaster rep2

description: RIL 58 male D. melanogaster

source_name: first legs of male D. melanogaster


title: proboscis of female D. melanogaster rep3

description: RIL 89 female D. melanogaster

tag: 4 pooled adult female individuals


title: male and female D. melanogaster embryos rep1

description: male and female D. melanogaster

source_name: male and female D. melanogaster embryos

tag: pooled male and female embryo individuals


title: offspring of cross of 128x89 female D. melanogaster, biological rep 2

description: cross 128x89 female D. melanogaster

source_name: cross 128x89 female D. melanogaster

tag: 20 pooled adult female individuals


title: offspring of cross of 128x89 male D. melanogaster, biological rep 2

description: cross 128x89 male D. melanogaster

source_name: cross 128x89 male D. melanogaster

tag: 20 pooled adult male individuals


title: proboscis of male D. melanogaster rep1

description: RIL 58 male D. melanogaster


title: first legs of male D. melanogaster rep1

description: RIL 89 male D. melanogaster


title: offspring of cross of 128x83 male D. melanogaster, biological rep 1

description: cross 128x83 male D. melanogaster

source_name: cross 128x83 male D. melanogaster


title: proboscis of male D. melanogaster rep3

description: RIL 89 male D. melanogaster

tag: 6 pooled adult male individuals


title: first legs of male D. melanogaster rep3

description: RIL 58 male D. melanogaster

tag: 12 pooled adult male individuals


title: offspring of cross of 134x128 female D. melanogaster, biological rep 2

description: cross 134x128 female D. melanogaster


title: male and female D. melanogaster embryos rep2

description: male and female D. melanogaster


title: antenna of male D. melanogaster rep4

description: RIL 89 male D. melanogaster


title: male and female D. melanogaster pupae rep2

description: male and female D. melanogaster

source_name: male and female D. melanogaster pupae

tag: pooled male and female pupae individuals


title: male and female D. melanogaster pupae rep3

description: male and female D. melanogaster


title: offspring of cross of 128x89 male D. melanogaster, biological rep 1

description: cross 128x89 male D. melanogaster


title: offspring of cross of 128x83 male D. melanogaster, biological rep 2

description: cross 128x83 male D. melanogaster


title: offspring of cross of 128x89 female D. melanogaster, biological rep 1

description: cross 128x89 female D. melanogaster


title: heads of male D. melanogaster rep3

description: male d. melanogaster

source_name: heads of male D. melanogaster rep

tag: pooled adult male individuals


title: offspring of cross of 128x83 female D. melanogaster, biological rep 1

description: cross 128x83 female D. melanogaster

source_name: cross 128x83 female D. melanogaster


title: offspring of cross of 128x83 female D. melanogaster, biological rep 2

description: cross 128x83 female D. melanogaster


title: heads of male D. melanogaster rep1

description: male d. melanogaster


title: offspring of cross of 128x75 male D. melanogaster, biological rep 1

description: cross 128x75 male D. melanogaster

source_name: cross 128x75 male D. melanogaster


title: male and female D. melanogaster pupae rep1

description: male and female D. melanogaster


title: offspring of cross of 128x75 female D. melanogaster, biological rep 1

description: cross 128x75 female D. melanogaster

source_name: cross 128x75 female D. melanogaster


title: offspring of cross of 128x58 female D. melanogaster, biological rep 1

description: cross 128x58 female D. melanogaster

source_name: cross 128x58 female D. melanogaster


title: offspring of cross of 128x75 male D. melanogaster, biological rep 2

description: cross 128x75 male D. melanogaster


title: offspring of cross of 128x58 male D. melanogaster, biological rep 2

description: cross 128x58 male D. melanogaster

source_name: cross 128x58 male D. melanogaster


title: offspring of cross of 128x23 male D. melanogaster, biological rep 2

description: cross 128x23 male D. melanogaster

source_name: cross 128x23 male D. melanogaster


title: offspring of cross of 128x58 female D. melanogaster, biological rep 2

description: cross 128x58 female D. melanogaster


title: offspring of cross of 128x23 male D. melanogaster, biological rep 1

description: cross 128x23 male D. melanogaster


title: male D. melanogaster pupae rep3

description: male d. melanogaster

source_name: male D. melanogaster pupae

tag: pooled male pupae individuals


title: offspring of cross of 128x75 female D. melanogaster, biological rep 2

description: cross 128x75 female D. melanogaster


title: offspring of cross of 128x58 male D. melanogaster, biological rep 1

description: cross 128x58 male D. melanogaster


title: male and female D. melanogaster pupae rep4

description: male and female D. melanogaster


title: offspring of cross of 128x23 female D. melanogaster, biological rep 2

description: cross 128x23 female D. melanogaster

source_name: cross 128x23 female D. melanogaster


title: male and female D. melanogaster embryos rep4

description: male and female D. melanogaster


title: offspring of cross of 128x17 male D. melanogaster, biological rep 2

description: cross 128x17 male D. melanogaster

source_name: cross 128x17 male D. melanogaster


title: male D. melanogaster pupae rep2

description: male d. melanogaster


title: offspring of cross of 128x17 male D. melanogaster, biological rep 1

description: cross 128x17 male D. melanogaster


title: offspring of cross of 128x23 female D. melanogaster, biological rep 1

description: cross 128x23 female D. melanogaster


title: abdomens of male D. melanogaster rep1

description: male d. melanogaster

source_name: abdomens of male D. melanogaster


title: female D. melanogaster pupae rep4

description: female D. melanogaster

source_name: female D. melanogaster pupae

tag: pooled female pupae individuals


title: heads of male D. melanogaster rep4

description: male d. melanogaster


title: male D. melanogaster pupae rep4

description: male d. melanogaster


title: offspring of cross of 128x145 female D. melanogaster, biological rep 2

description: cross 128x145 female D. melanogaster

source_name: cross 128x145 female D. melanogaster


title: offspring of cross of 128x17 female D. melanogaster, biological rep 1

description: cross 128x17 female D. melanogaster

source_name: cross 128x17 female D. melanogaster


title: female D. melanogaster pupae rep2

description: female D. melanogaster


title: offspring of cross of 128x17 female D. melanogaster, biological rep 2

description: cross 128x17 female D. melanogaster


title: offspring of cross of 128x145 male D. melanogaster, biological rep 1

description: cross 128x145 male D. melanogaster

source_name: cross 128x145 male D. melanogaster


title: female D. melanogaster pupae rep1

description: female D. melanogaster


title: female D. melanogaster pupae rep3

description: female D. melanogaster


title: offspring of cross of 128x134 female D. melanogaster, biological rep 2

description: cross 128x134 female D. melanogaster

source_name: cross 128x134 female D. melanogaster


title: offspring of cross of 128x145 female D. melanogaster, biological rep 1

description: cross 128x145 female D. melanogaster


title: offspring of cross of 128x134 female D. melanogaster, biological rep 1

description: cross 128x134 female D. melanogaster


title: abdomens of male D. melanogaster rep4

description: male d. melanogaster


title: offspring of cross of 128x134 male D. melanogaster, biological rep 2

description: cross 128x134 male D. melanogaster

source_name: cross 128x134 male D. melanogaster


title: male and female D. melanogaster embryos rep3

description: male and female D. melanogaster

source_name: male and female D. melanogaster embryos


title: abdomens of male D. melanogaster rep2

description: male d. melanogaster


title: offspring of cross of 128x145 male D. melanogaster, biological rep 2

description: cross 128x145 male D. melanogaster


title: male D. melanogaster pupae rep1

description: male d. melanogaster


title: Antenna of female D. melanogaster rep4

description: RIL 89 female D. melanogaster

source_name: Antenna of female D. melanogaster


title: Antenna of female D. melanogaster rep3

description: RIL 89 female D. melanogaster


title: offspring of cross of 128x134 male D. melanogaster, biological rep 1

description: cross 128x134 male D. melanogaster


title: WT1(-KTS) expression induced for 8 hrs 01-14-03_Lee_U133A_8_2

status: Public on Jun 21 2007

city: Bethesda

first: WeiPing

last: Chen

state: MD

address: Bldg 8, Room 1A11, NIDDK/NIH

department: GCL

institute: NIDDK/NIH

description: WT1(-KTS) expression was induced for 8 hrs and the expression profile of each time-points was compared to the uninduced sample (0 hr).

data_processing: Affymetrix Microarray Suite version 5.0

tag: U2OS cell line(osteosarcoma;female),Tetracycline-repressible WT1(-KTS) expression, time-course induction of WT1(-KTS) expression.


title: WT1(-KTS) expression induced for 4 hrs 01-14-03_Lee_U133A_4_2

description: WT1(-KTS) expression was induced for 4hrs and the expression profile of each time-points was compared to the uninduced sample (0 hr).

tag: 2OS cell line(osteosarcoma;female),Tetracycline-repressible WT1(-KTS) expression, time-course induction of WT1(-KTS) expression


title: WT1(-KTS) expression induced for 12 hrs 01-14-03_Lee_U133A_12_2

description: WT1(-KTS) expression was induced for 12 hrs and the expression profile of each time-points was compared to the uninduced sample (0 hr)

source_name: 12_2 cells with Tetracycline-repressible WT1(-KTS) expression

tag: U2OS cell line(osteosarcoma;female),Tetracycline-repressible WT1(-KTS) expression, time-course induction of WT1(-KTS) expression


title: proboscis of female D. melanogaster rep2

description: RIL 58 female D. melanogaster

source_name: proboscis of female D. melanogaster

tag: 5 pooled adult female individuals


title: antenna of male D. melanogaster rep1

description: RIL 89 male D. melanogaster

source_name: antenna of male D. melanogaster


title: heads of male D. melanogaster rep2

description: male d. melanogaster

source_name: heads of male D. melanogaster rep


title: first legs of female D. melanogaster rep4

description: RIL 89 female D. melanogaster

source_name: first legs of female D. melanogaster


title: first legs of male D. melanogaster rep4

description: RIL 89 male D. melanogaster

source_name: first legs of male D. melanogaster


title: Uninduced control 01-14-03_Lee_U133A_Tet

description: WT1(-KTS) expression was induced for the uninduced sample (0 hr). This is the control.

source_name: Tet cells with Tetracycline-repressible WT1(-KTS) expression


title: Mn 200 uM batch 1

status: Public on Jun 14 2007

city: New York

first: Sarah

last: Mense

state: NY

address: 722 West 168th Street

department: Environmental Health Sciences

institute: Columbia University

description: astrocytes from second trimester (14-19 weeks) human fetal brains

hyb_protocol: standard Affymetrix procedures

scan_protocol: standard Affymetrix procedures

source_name: Mn 200 uM batch 1

tag: astrocytes from second trimester human fetal brains


title: RTS II cell line stimulation 1 (SsamcellB1a)

status: Public on Jun 16 2007

city: Aberdeen

first: Sam

middle: A

last: Martin

state: Scotland

address: Tillydrone Avenue

department: Zoology

country: United Kingdom

institute: University of Aberdeen

description: Rainbow trout cell line RTS II stimulated with recombinant Interleukin 1 beta for 6 hours

hyb_protocol: Prior to hybridization 1.5mL d(A)80 (1mg ml-1), 110mL formamide, 55ul hybridization buffer (Amersham Biosciences) and water was added to give a final volume of 220 ml.  The cDNA was heated to 95°C for 2 min before being added to the hybridization chamber.  The hybridization was performed for 16 h at 42°C on an Amersham Lucidea hybridization station. Prior to hybridization slides were pretreated by heating in water at 95°C for 5 min, then washed with isopropanol and air dried.  Washes were as follows:  1X SSC 10 min, 1X SSC +0.2% SDS 10 min, 0.1 X SSC + 0.2% SDS 10 min.  Slides were then dried before being scanned.

scan_protocol: Images were scanned using Axon 4100B scanner.  Resolution was at 10mM.  Gel images were edited using GenePix Version 5.1.

data_processing: Initial image analysis was performed with the GenPix (version 5.1, Axon instruments) program; the array images were edited to ensure that the GAL (Gene Associated List) file was correctly orientated and that any abnormal hybridization signals were flagged as bad.  Edited images were imported into Acuity (version 5.1, Axon instruments) and analysis performed.  All arrays were background corrected using local background correction and normalized using Lowess normalization. The data was filtered to remove bad signal and signal that did not reach a predetermined threshold (signal to noise ratio >1.5 for either Cy3 or Cy5).  Data in VALUE column is RAW data and not normalized

organism: Oncorhynchus mykiss

source_name: RTS II cells stimulated with recombinant interleukin 1 beta

label_protocol: Labelled cDNA was generated using Amersham Pharmacia post labelling kit.  Total RNA (20mg) was used as template for each labelling.

extract_protocol: RNAstat (TEL_TEST INC.)  According to manufacturers instructions.

source_name: RTS II cells Non stimulated

tag: RTS II cells Non stimulated


title: Mn 200 uM batch 3

source_name: Mn 200 uM batch 3


title: Mn 200 uM batch 2

source_name: Mn 200 uM batch 2


title: harmane 1uM batch 3

source_name: harmane 1uM batch 3


title: proboscis of female D. melanogaster rep4

description: RIL 89 female D. melanogaster


title: none batch 3

source_name: none batch 3


title: Antenna of female D. melanogaster rep1

description: RIL 58 female D. melanogaster


title: antenna of male D. melanogaster rep3

description: RIL 58 male D. melanogaster


title: first legs of female D. melanogaster rep2

description: RIL 58 female D. melanogaster

tag: 10 pooled adult female individuals


title: Antenna of female D. melanogaster rep2

description: RIL 58 female D. melanogaster


title: none batch 2

source_name: none batch 2


title: none batch 1

source_name: none batch 1


title: harmane 1uM batch 2

source_name: harmane 1uM batch 2


title: Healthy donor SN_45_vs_Pool

status: Public on Jun 06 2007

city: MADRID

first: Luis

last: LOMBARDIA

state: Madrid

address: Melchor Fernandez Almagro, 3

department: Molecualr Pathology Programme

country: Spain

institute: CNIO (Spanish National Cancer Center)

description: Informed consent was obtained from all participants following an explanation of the nature of the study, as approved by the research ethics board of our hospital.

hyb_protocol: Prehybridization was performed at 42ºC for 30-45 minutes in 6X SSC, 0.5% SDS and 1% BSA. Slides were rinsed five times with destilled water. Cy5 and Cy3 amplified RNA (aRNA) probes were mixed (30-50 pmol of each label) with 10 µg of PolyA (Sigma) and 1 µg of Cot-DNA (Invitrogen) in a final volume of 40 µl of hybridization buffer (50% formamide, 6X SSC, 0.5% SDS, 5X Denhardt’s). The probe was denatured at 95ºC for 5 minutes and applied to the slide using a coverslip. Slides were then incubated at 42ºC for 16h in hybridization chambers (Array-It). After incubation, slides were washed twice with 0.1X SSC, 0.1% SDS for 5 minutes each, three times with 0.1X SSC for 5 minutes and finally in distilled water for 10 seconds. Slides were dried by centrifugation at 563g for 1 minute.

scan_protocol: Images from Cy3 and Cy5 channels were equilibrated and captured with an Axon 4000B scanner (Axon Instruments) and spots quantified using GenePix 5.1 software (Axon). GPR files were then included in almaZen database software (Bioalma). Signals were background subtracted, and normalized by total intensity and lowess_pritn_tip_group.

data_processing: GPR files with raw data were introduced in almaZen database software (Bioalma). Signals were background subtracted, and normalized by total intensity and Print_tip_group_Lowess.

molecule: polyA RNA

label_protocol: aRNA was generated in the second amplification round including Cy3-aminoallyl-UTP for labeling by using CyDye PostLabelling Reactive Dye Pack (GE Healthcare) and following Ambion kit instructions. Labeled aRNA was measured in a Nanodrop1000.

extract_protocol: Plasma mRNA was obtained from 3 ml of sample using Dynabeads mRNA DIRECT Kit following manufacturer´s instructions. In brief, plasma was incubated with 200 ul of Dynabeads Oligo (dT) for 10 min at room temperature and the mRNA was eluted in 10 mM Tris-HCl. Since low amounts of mRNA were obtained, mRNA was 2-cycle amplified by Eberwine protocol using AminoAllyl MessageAmp aRNA kit (Ambion) following manufacturer instructions.

treatment_protocol: Blood sample (10 ml) was taken from healthy donor by venipuncture before intervention on the day of surgery, and the first several milliliters were discarded to eliminate skin-plug contamination. Plasma was prepared by centrifugation of peripheral blood at 2500 rpm for 25 minutes and divided into aliquots, which were snap frozen at -80ºC until processing.

tag: Blood sample from healthy donor

source_name: Normal_Control pool

label_protocol: aRNA was generated in the second amplification round including Cy5-aminoallyl-UTP for labeling by using CyDye PostLabelling Reactive Dye Pack (GE Healthcare) and following Ambion kit instructions. Labeled aRNA was measured in a Nanodrop1000.

treatment_protocol: Plasma was prepared by centrifugation of peripheral blood at 2500 rpm for 25 minutes and snap frozen at -80ºC until processing.

tag: Pool of blood samples from 26 healthy donors, obtained at the hematology unit of the hospital.


title: SW756

status: Public on Jun 12 2007

type: genomic

first: William

middle: Wallace

last: Lockwood

address: 50 South Drive

institute: National Human Genome Research Institute

description:
cyanine 3 = sample
cyanine 5 = reference

data_processing: Normalized log2 ration used method according Khojasteh et al BMC Bioinformatics 2005;6:274

label: Cyanine 3

molecule: genomic DNA

source_name: SW756 Cervical Cancer Cell Line

tag: ATCC #CRL-10302

label: cyanine 5

source_name: Female Genomic

tag: NOVAGEN POOLED FEMALE REFERENCE GENOMIC DNA


title: harmane 1uM batch 1

source_name: harmane 1uM batch 1


title: Healthy donor SN_53_vs_Pool


title: Healthy donor SN31_vs_Pool


title: Colorectal cancer patient SC14_vs_Pool

description: Informed consent was obtained from all participants following an explanation of the nature of the study, as approved by the research ethics board of our hospital. All patients were considered sporadic cases on the basis that no clinical antecedents of familial adenomatous polyposis were reported, and those that met the clinical criteria for hereditary non-polyposis colon cancer (Amsterdam criteria) were excluded.

treatment_protocol: Blood sample (10 ml) was taken from the patient with colorectal carcinoma by venipuncture before intervention on the day of surgery, and the first several milliliters were discarded to eliminate skin-plug contamination. Plasma was prepared by centrifugation of peripheral blood at 2500 rpm for 25 minutes and divided into aliquots, which were snap frozen at -80ºC until processing.

tag: Blood sample from patients considered as Colorectal cancer sporadic case on the basis that no clinical antecedents of familial adenomatous polyposis were reported.


title: SiHa

source_name: SiHa Cervical Cancer Cell Line

tag: ATCC #HTB-35


title: Healthy donor SN_19_vs_Pool


title: Colorectal cancer patient SC16_vs_Pool


title: Healthy donor SN9_vs_Pool


title: MS751

source_name: MS751 Cervical Cancer Cell Line

tag: ATCC #HTB-34


title: Colorectal cancer patient SC18_vs_Pool


title: Colorectal cancer patient SC15_vs_Pool


title: Healthy donor SN_10_vs_Pool


title: Colorectal cancer patient SC8_vs_Pool


title: Colorectal cancer patient SC5_vs_Pool


title: Colorectal cancer patient SC7_vs_Pool


title: Healthy donor SN_28_vs_Pool


title: Colorectal cancer patient SC3_vs_Pool


title: ME-180

source_name: ME-180 Cervical Cancer Cell Line

tag: ATCC #HTB-33


title: abdomens of male D. melanogaster rep3

description: male d. melanogaster

source_name: abdomens of male D. melanogaster

tag: pooled adult male individuals


title: Colorectal cancer patient SC9_vs_Pool


title: expression_8004#-XVM2_8004#-NYG_2

status: Public on Jan 01 2007

city: Beijing

first: Liang

last: Zhang

address: 18 Life Science Parkway,Changping District

country: China

institute: CapitalBio Corporation

description: Comparativethe expression profiles profiling of bacteria harvested from the rich medium NYG and the defined medium XVM2

data_processing: After background correction and removal of bad spots, expression ratio was linear normalized based on the gene XC3300

organism: Xanthomonas campestris pv. campestris

source_name: strain 8004#-NYG

tag: 8004# strain,total RNA, harvested from the rich medium NYG

source_name: strain 8004#-XVM2

tag: 8004# strain,total RNA, harvested from the defined medium XVM2


title: Ca Ski

source_name: Ca Ski Cervical Cancer Cell Line

tag: ATCC #CRL-1550


title: expression_8004#-XVM2_8004#-NYG_1


title: CGH_1# vs 8004#_1

description: Comparative genome analysis the genomic diversity and host preferences in Xanthomonas campestris pv. campestris

source_name: strain 8004# gDNA

tag: Strain 8004#

source_name: strain 1# gDNA

tag: Strain 1#


title: CGH_8004# vs 8004#_2


title: CGH_15# vs 8004#_1

source_name: strain 15# gDNA

tag: Strain 15#


title: first legs of female D. melanogaster rep1

description: RIL 58 female D. melanogaster

source_name: first legs of female D. melanogaster

tag: 20 pooled adult female individuals


title: CGH_18# vs 8004#_1

source_name: strain 18# gDNA

tag: Strain 18#


title: CGH_20# vs 8004#_1

source_name: strain 20# gDNA

tag: Strain 20#


title: CGH_17# vs 8004#_1

source_name: strain 17# gDNA

tag: Strain 17#


title: CGH_33913# vs 8004#_1

source_name: strain 33913# gDNA

tag: Strain 33913#


title: CGH_8004# vs 8004#_1


title: Colorectal cancer patient SC13_vs_Pool

source_name: Normal_Control pool

tag: Pool of blood samples from 26 healthy donors, obtained at the hematology unit of the hospital.


title: CGH_12# vs 8004#_2

source_name: strain 12# gDNA

tag: Strain 12#


title: C-4 I

source_name: C-4 I Cervical Cancer Cell Line

tag: ATCC #CRL-1594

source_name: Female Genomic

tag: NOVAGEN POOLED FEMALE REFERENCE GENOMIC DNA


title: CGH_12# vs 8004#_1


title: HeLa

source_name: HeLa Cervical Cancer Cell Line

tag: ATCC #CCL-2


title: CGH_14# vs 8004#_1

source_name: strain 14# gDNA

tag: Strain 14#


title: DoTc2 4510

source_name: DoTc2 4510 Cervical Cancer Cell Line

tag: ATCC #CRL-7920


title: CGH_9# vs 8004#_1

source_name: strain 9# gDNA

tag: Strain 9#


title: CGH_16# vs 8004#_1

source_name: strain 16# gDNA

tag: Strain 16#


title: CGH_11# vs 8004#_1

source_name: strain 8004# gDNA

tag: Strain 8004#

source_name: strain 11# gDNA

tag: Strain 11#


title: CGH_5# vs 8004#_2

source_name: strain 5# gDNA

tag: Strain 5#


title: CGH_5# vs 8004#_1


title: CGH_8# vs 8004#_2

source_name: strain 8# gDNA

tag: Strain 8#


title: CGH_7# vs 8004#_1

source_name: strain 7# gDNA

tag: Strain 7#


title: CGH_8# vs 8004#_1


title: CGH_6# vs 8004#_1

source_name: strain 6# gDNA

tag: Strain 6#


title: CGH_3# vs 8004#_1

source_name: strain 3# gDNA

tag: Strain 3#


title: CGH_2# vs 8004#_1

source_name: strain 2# gDNA

tag: Strain 2#


title: CGH_6# vs 8004#_2


title: CGH_10# vs 8004#_1

source_name: strain 10# gDNA

tag: Strain 10#


title: Healthy donor SN61_vs_Pool

tag: Blood sample from healthy donor

source_name: Normal_Control pool

tag: Pool of blood samples from 26 healthy donors, obtained at the hematology unit of the hospital.


title: CGH_2# vs 8004#_2


title: smoker 3

status: Public on Dec 31 2006

city: Jena

first: Sandy

last: Mosig

address: Bachstrasse 18

department: Molecular Hemostaseology - Institute of Vascular Medicine

country: Germany

institute: University Hospital Jena

description: Gene expression data from the smoking participant  3

hyb_protocol: Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG U133A2.0 GeneChips were washed and stained in the Affymetrix Fluidics Station 400.

scan_protocol: GeneChips were scanned using the Affymetrix GeneChip Scanner 3000

data_processing: The data were analyzed with GCOS 1.4 using Affymetrix default analysis settings and global scaling as normalization method.

source_name: human CD3 positive T cells

label_protocol: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 microg total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).

extract_protocol: the RNA was worked up using PLG Tubes and the Qiagen Rneasy Kit

treatment_protocol: T -lymphocytes were precipitated untouched from 4ml peripheral blood mononuclear cells, washed in PBS + 2mM EDTA, lysed in TriZol

tag: smoker


title: smoker 2

description: Gene expression data from the smoking participant  2


title: control nonsmoker 1

description: Gene expression data from the non smoking participant 1

tag: nonsmoker


title: control nonsmoker 2

description: Gene expression data from the non smoking participant 2


title: female MhmBN chip 46

city: Mannheim

first: Li

last: Li

address: Theodor-Kutzer-Ufer

institute: University Heidelberg

description: pooled RNA of the kidney of 36 day old rats

data_processing: SAS Microarray Solution 1.0

organism: Rattus norvegicus

source_name: female MhmBN

label_protocol: Approximately 15 µg of pooled total RNA was processed to produce biotinylated cRNA targets.

extract_protocol: total RNA were isolated from the left kidney of 54 rats at the age of 36 days, using the Trizol-reganet from Invitrogen.

tag: kidney


title: control nonsmoker 3

description: Gene expression data from the non smoking participant  3


title: smoker 1

description: Gene expression data from the smoking participant 1


title: CGH_4# vs 8004#_1

source_name: strain 4# gDNA

tag: Strain 4#


title: female MhmBN chip 45


title: female MhmBN chip 47


title: female us chip 58

source_name: female us


title: female mhm chip 42

source_name: female mhm


title: female us chip 57


title: male us chip 37

source_name: male us


title: Untreated cells  pc12 tmt contr_2_Signal

city: Rome

first: camilla

last: bernardini

address: L.go F.Vito 1

department: Human Anatomy

country: Italy

institute: UCSC

description: untreated cells  pc12 tmt contr_2_Signal

hyb_protocol: Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip RAT 230A Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.

scan_protocol: GeneChips were scanned using the Agilent GeneArray Scanner 2500

data_processing: Raw data have been normalized using gc-RMA methods, followed  by an additional “per gene normalisation” the data for a given gene is normalised to the median expression level of that gene across all samples.

source_name: commercial cell line

label_protocol: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).

growth_protocol: cells were cultured in complete DMEM till 80% confluence, then placed at 5000 cells per squared cm

extract_protocol: Rneasy mini kit  extraction of total RNA was performed according to the manufacturer's instructions.

treatment_protocol: no treatment (cells treated with cell culture medium alone)

tag: rat pheocromocitoma cell line


title: male MhmBN chip 25

source_name: male MhmBN


title: female us chip 4

source_name: female us chip 4


title: male mhm chip 21

source_name: male mhm


title: male us chip 38


title: High concentration treated cells  pc12 5uM_Signal

description: high concentration treated cells  pc12 5uM_Signal

treatment_protocol: cells were treated with 5uM Trimethyltin (TMT) in cell culture medium


title: High concentration treated cells  pc12 tmt 5uM_5_Signal

description: high concentration treated cells  pc12 tmt 5uM_5_Signal


title: Low concentration treated cells  pc12 1uM_Signal

description: low concentration treated cells  pc12 1uM_Signal

treatment_protocol: cells were treated with 1uM Trimethyltin (TMT) in cell culture medium


title: male MhmBN chip 27


title: High concentration treated cells  pc12 tmt 5uM_6_Signal

description: high concentration treated cells  pc12 tmt 5uM_6_Signal


title: female mhm chip 41


title: 4_P2_A

city: Berlin

first: Henning

last: Witt

address: Ihnestrasse 65

department: Hans Lehrach

institute: Max Planck Institute for Molecular Genetics

description: stroke operated animals with candesartan pre-treatment

hyb_protocol: Fifteen micrograms of each cRNA sample was hybridized for 16 hours at 45°C to rat expression Affymetrix RAE 230A GeneChip arrays. Following the hybridization arrays were washed and stained with streptavidin-phycoerythrin using a fluidics station in accordance with the Affymetrix protocol.

scan_protocol: Affymetrix GeneChip System confocal scanner 2500

data_processing: Affymetrix GeneChip Operating Software (GCOS)

label: biotin labeled

source_name: stroke and candesartan

label_protocol: The amplification and labeling of the RNA samples were carried out according to the manufacturer's instructions (Affymetrix, Santa Clara, CA). Briefly, total RNA was quantified by UV-spectroscopy and its quality evaluated by analysis on a LabChip (BioAnalyzer, AGILENT Technologies, Santa Clara, CA). In the presence of an oligo(dT)24 primer containing a T7 RNA polymerase promoter (TIBMOL Biol, Berlin, Germany) one to three micrograms of total RNA were used for double-stranded cDNA synthesis (SuperScript transcriptase, Life Technologies, Inc., Carlsbad, CA). Labeled complementary RNA (cRNA) was prepared from double-stranded cDNA by in vitro transcription using the GeneChip RNA transcript labeling kit (Affymetrix, Santa Clara, CA). After cleanup (Qiagen, Hilden, Germany), the biotin-labeled cRNA was fragmented by alkaline treatment (40 mM Tris-acetate (pH 8.2), 100 mM potassium acetate, and 50 mM magnesium acetate) at 94°C for 35 minutes.

growth_protocol: Animals were kept in a SPF barrier under standardisized conditions with respect to temperature and humidity, and were housed on a 12h light/12h dark cycle in groups of 4-5 with free access to food and water. Animal housing, care, and applications of experimental procedures complied with the Guide for the Care and Use of Laboratory Animals of the Government of Berlin, Germany and are in accordance to the recommendations of the Society for Laboratory Animal Science (GV-SOLAS) and the Federation of European Laboratory Animal Science Associations (FELASA).

extract_protocol: Total RNA was isolated 2 h after MCAO from a segment of the right hemisphere of the brain using TRIzol Reagent (Invitrogen).

treatment_protocol: stroke operated: Five days after beginning of treatment, focal cerebral ischemia was induced by right middle cerebral artery occlusion (MCAO) for 90min  with subsequent reperfusion as described previously (Groth et al., 2003; Dai et al., 1999).

tag: strain

value: male wistar rats

tag: weight

tag: age

value: 7 weeks


title: 24h-3

city: Chapel Hill

first: Monte

middle: S

last: Willis

state: NC

address: 103 Mason Farm Road

department: Pathology & Laboratory Medicine

institute: UNC

description: MEC cell line

data_processing: quantile normalization of raw intensities, and log2 transformed ratios

source_name: MEC cell line treated with BMP6 for 24h

extract_protocol: Agilent

tag: MEC cell line

source_name: Universal mouse reference RNA

tag: Universal mouse reference RNA


title: male mhm chip 1


title: male us chip 2


title: Low concentration treated cells  pc12 tmt 1uM_3_Signal

description: low concentration treated cells  pc12 tmt 1uM_3_Signal


title: Low concentration treated cells  pc12 tmt 1uM_4_Signal

description: low concentration treated cells  pc12 tmt 1uM_4_Signal


title: Untreated cells  pc12 tmt contr_1_Signal

description: untreated cells  pc12 tmt contr_1_Signal


title: Untreated cells  pc12 contr_Signal

description: untreated cells  pc12 contr_Signal


title: 3_P1_A

description: sham operated animals with candesartan pre-treatment

source_name: sham and candesartan

extract_protocol: Total RNA was isolated 2 h after sham from a segment of the right hemisphere of the brain using TRIzol Reagent (Invitrogen).

treatment_protocol:
sham operated,
pre-treatment: Candesartan (0.1 mg/kg bwt) twice daily s.c. for five days


title: 12h-1

description: MEC cell line

source_name: MEC cell line treated with BMP6 for 12h


title: 4h-3

description: MEC cell line

source_name: MEC cell line treated with BMP6 for 4h


title: 2_P1_A

description: stroke operated animals with vehicle pre-treatment

source_name: stroke and vehicle

treatment_protocol:
stroke operated: Five days after beginning of treatment, focal cerebral ischemia was induced by right middle cerebral artery occlusion (MCAO) for 90min with subsequent reperfusion as described previously (Groth et al., 2003; Dai et al., 1999).
pre-treatment: vehicle (0.9% NaCl) twice daily s.c. for five days


title: female mhm chip 3


title: 24h-1

description: MEC cell line


title: 12h-2

description: MEC cell line


title: 4h-2

description: MEC cell line


title: male mhm chip 22


title: C-2

description: MEC cell line

source_name: MEC cell line (control)


title: 3_P2_A

treatment_protocol:
sham operated,
pre-treatment: Candesartan (0.1mg/kg bw) twice daily for five days


title: C-1

description: MEC cell line


title: 12h-3

description: MEC cell line


title: Ssn6 Overexpression L040B sl20

status: Public on Nov 15 2006

city: Huddinge

first: Anthony

last: Wright

address: Blickagången 6

country: Sweden

institute: Karolinska Institute

description: We have performed a genome wide expression profile study of the essential transcriptional co-repressor protein Ssn6 in Schizosaccharomyces pombe. Ssn6 affected targets were identified by over expression of Ssn6 protein in a WT strain. A WT strain holding a plasmid expressing Ssn6 (pDUAL-Ssn6) was compared against the expression in a WT with the empty plasmid (pDUAL-EMPTY) strain under selection for the plasmids MM-URA media at 30 degrees (Xue et al, 2004).

hyb_protocol: Xue et al, 2004

scan_protocol: Scanexpress Laser scanner

data_processing: Genespring Silicon Genetics Software

organism: Schizosaccharomyces pombe

source_name: WT-pDUAL-Empty

label_protocol: Xue et al, 2004

growth_protocol: Xue et al, 2004

extract_protocol: Xue et al, 2004

treatment_protocol: Xue et al, 2004

tag: WT(FY367)-pDUAL-Empty MM-URA 30 Deg

tag: WT(FY367)-pDUAL-Ssn6 MM-URA 30 Deg


title: 1_P2_A

description: sham operated animals with vehicle pre-treatment

source_name: sham and vehicle

treatment_protocol:
sham operated,
pre-treatment: vehicle (0.9% NaCl) twice daily s.c. for five days


title: 1_P1_A


title: 4_P1_A

treatment_protocol:
stroke operated: Five days after beginning of treatment, focal cerebral ischemia was induced by right middle cerebral artery occlusion (MCAO) for 90min with subsequent reperfusion as described previously (Groth et al., 2003; Dai et al., 1999).
pre-treatment: Candesartan (0.1mg/kg bw) twice daily for five days


title: 2_P2_A


title: 24h-2

description: MEC cell line


title: 4h-1

description: MEC cell line