title: HKCO03N

description:
Colon tissue samples for DNA microarrays; Samples of tumor and normal colon mucosa were collected from colon cancer resection from Department of Surgery, Queen Mary Hospital University of Hong Kong. Tissue was frozen in liquid nitrogen within 30 min of resection. Nonneoplastic mucosa from colon was dissected free of muscle and histologically confirmed to be tumor free by frozen section. Total RNA was extracted using Trizol (Invitrogen, Carlsbad, CA) from each tissue sample and processed for microarray hybridization.; Protocol Type = Biological Sample; Performer: Xin,,Chen
Simple annotation: Primary tumor, Colon Cancer
Image: http://smd.stanford.edu/MicroArray/gifs/2003-11/46628.gif
Disease State: Healthy Normal Colon sample

scan_protocol: Array images were scanned by using an Axon Scanner 4000B, and data were analyzed by using GenePix 3.0 (Axon Instruments, Union City, CA). Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 850, 5040; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Xin,,Chen Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.36; Parameter Scan Time = 23:30:26; Parameter PMT Volts = 610; Parameter Scan Date = 2003-11-11; Parameter Pixel Size = 10; Parameter Laser on time = 134523; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 3.26; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Xin,,Chen Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.36; Parameter Scan Time = 23:30:26; Parameter PMT Volts = 680; Parameter Scan Date = 2003-11-11; Parameter Pixel Size = 10; Parameter Laser on time = 353494; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.93; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Xin,,Chen

source_name: amplified HKCO03N

tag: amplified HKCO03N


title: Expt189 MCM263 vs W6G 3

status: Public on Aug 16 2007

type: genomic

city: Stanford

last: Stanford Microarray Database

state: CA

address: 300 Pasteur Drive

department: Stanford University, School of Medicine

country: USA

institute: Stanford Microarray Database (SMD)

description:
Simple annotation: Genome comparison, Map Recombination Event
Image: http://smd.stanford.edu/MicroArray/gifs/2005-10/64614.gif

hyb_protocol: not provided

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 370, 27620; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Michael,,Miller Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.73; Parameter Scan Time = 14:29:27; Parameter PMT Volts = 480; Parameter Scan Date = 2005-10-16; Parameter Pixel Size = 10; Parameter Laser on time = 55381; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 2.55; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.3.1; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.73; Parameter Scan Time = 14:29:27; Parameter PMT Volts = 740; Parameter Scan Date = 2005-10-16; Parameter Pixel Size = 10; Parameter Laser on time = 201305; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 1.38; Parameter Scanning software version = 2.0.3.1; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller

data_processing: VALUE is Log (base 2) of the ratio of the median of Channel 2 (usually 635 nm) to Channel 1 (usually 532 nm)

molecule: genomic DNA

organism: Vibrio cholerae

label_protocol: not provided

extract_protocol: not provided


title: Expt189 MCM263 vs W6G 2

description:
Simple annotation: Genome comparison, Map Recombination Event
Image: http://smd.stanford.edu/MicroArray/gifs/2005-10/64613.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 370, 27620; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Michael,,Miller Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.68; Parameter Scan Time = 14:19:40; Parameter PMT Volts = 490; Parameter Scan Date = 2005-10-16; Parameter Pixel Size = 10; Parameter Laser on time = 55371; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 2.55; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.3.1; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.68; Parameter Scan Time = 14:19:40; Parameter PMT Volts = 730; Parameter Scan Date = 2005-10-16; Parameter Pixel Size = 10; Parameter Laser on time = 201295; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 1.38; Parameter Scanning software version = 2.0.3.1; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller


title: 14D S3_13a

status: Public on Aug 17 2007

type: RNA

city: Stockholm

first: Annika

last: Saaf

department: Department of Molecular Medicine & Surgery

country: Sweden

institute: Karolinska Institute, Karolinska University Hospital, Solna

description:
Simple annotation: epithelial cells, Caco-2
Image: http://smd.stanford.edu/MicroArray/gifs/2003-01/37044.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1920, 7480; Parameter Datafile type = GenePix Results 1.4; Software: type: feature extraction; Performer: Annika,,Saaf Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 29.53; Parameter Scan Time = 10:17:26; Parameter PMT Volts = 580; Parameter Scan Date = 2002-12-11; Parameter Pixel Size = 10; Parameter Laser on time = 44208; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 4.74; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Annika,,Saaf Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 29.53; Parameter Scan Time = 10:17:26; Parameter PMT Volts = 610; Parameter Scan Date = 2002-12-11; Parameter Pixel Size = 10; Parameter Laser on time = 263203; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 3.18; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Annika,,Saaf

molecule: total RNA

organism: Homo sapiens

source_name: Stratagene Human amp reference 7/30/02

tag: Stratagene Human amp reference 7/30/02

tag: Time


title: Expt189 MCM259 vs W6G 3

description:
Simple annotation: Genome comparison, Map Recombination Event
Image: http://smd.stanford.edu/MicroArray/gifs/2005-10/64610.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 970, 28520; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Michael,,Miller Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.56; Parameter Scan Time = 13:47:06; Parameter PMT Volts = 490; Parameter Scan Date = 2005-10-16; Parameter Pixel Size = 10; Parameter Laser on time = 55338; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 2.55; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.3.1; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.56; Parameter Scan Time = 13:47:06; Parameter PMT Volts = 750; Parameter Scan Date = 2005-10-16; Parameter Pixel Size = 10; Parameter Laser on time = 201263; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 1.37; Parameter Scanning software version = 2.0.3.1; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller


title: Expt189 MCM257 vs W6G 3

description:
Simple annotation: Genome comparison, Map Recombination Event
Image: http://smd.stanford.edu/MicroArray/gifs/2005-10/64609.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 970, 28140; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Michael,,Miller Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.47; Parameter Scan Time = 13:25:45; Parameter PMT Volts = 500; Parameter Scan Date = 2005-10-16; Parameter Pixel Size = 10; Parameter Laser on time = 55317; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 2.56; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.3.1; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.47; Parameter Scan Time = 13:25:45; Parameter PMT Volts = 750; Parameter Scan Date = 2005-10-16; Parameter Pixel Size = 10; Parameter Laser on time = 201242; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 1.37; Parameter Scanning software version = 2.0.3.1; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller


title: HKCO10T

description:
Colon tissue samples for DNA microarrays; Samples of tumor and normal colon mucosa were collected from colon cancer resection from Department of Surgery, Queen Mary Hospital University of Hong Kong. Tissue was frozen in liquid nitrogen within 30 min of resection. Nonneoplastic mucosa from colon was dissected free of muscle and histologically confirmed to be tumor free by frozen section. Total RNA was extracted using Trizol (Invitrogen, Carlsbad, CA) from each tissue sample and processed for microarray hybridization.; Protocol Type = Biological Sample; Performer: Xin,,Chen
Simple annotation: Primary tumor, Colon Cancer
Image: http://smd.stanford.edu/MicroArray/gifs/2003-11/46786.gif
Disease State: Tumor Colon Samples

scan_protocol: Array images were scanned by using an Axon Scanner 4000B, and data were analyzed by using GenePix 3.0 (Axon Instruments, Union City, CA). Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1150, 5010; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Xin,,Chen Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.51; Parameter Scan Time = 23:28:02; Parameter PMT Volts = 610; Parameter Scan Date = 2003-11-13; Parameter Pixel Size = 10; Parameter Laser on time = 135211; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 3.28; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Xin,,Chen Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.51; Parameter Scan Time = 23:28:02; Parameter PMT Volts = 720; Parameter Scan Date = 2003-11-13; Parameter Pixel Size = 10; Parameter Laser on time = 354182; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.93; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Xin,,Chen

source_name: amplified CRH

tag: amplified CRH

source_name: amplified HKCO10T

label_protocol: Fluorescently labeled cDNA prepared from RNA linearly amplified using MessageAmp (Ambion) was hybridized to the array in a two-color comparative format, with normal colon or colon cancer samples labeled with Cy-5, and a reference pool of human mRNAs labelled with Cy-3 (Stratagene, Cat #740000).

tag: amplified HKCO10T


title: Expt189 MCM254 vs W6G 3

description:
Simple annotation: Genome comparison, Map Recombination Event
Image: http://smd.stanford.edu/MicroArray/gifs/2005-10/64607.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1060, 27760; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Michael,,Miller Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.30; Parameter Scan Time = 13:00:29; Parameter PMT Volts = 500; Parameter Scan Date = 2005-10-16; Parameter Pixel Size = 10; Parameter Laser on time = 55291; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 2.55; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.3.1; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.30; Parameter Scan Time = 13:00:29; Parameter PMT Volts = 730; Parameter Scan Date = 2005-10-16; Parameter Pixel Size = 10; Parameter Laser on time = 201216; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 1.36; Parameter Scanning software version = 2.0.3.1; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller


title: 10D S2 TC 5/3/02

description:
Simple annotation: epithelial cells, Caco-2
Image: http://smd.stanford.edu/MicroArray/gifs/2002-10/34510.gif

scan_protocol: not provided

source_name: Staratagene amp reference 7/30/02

tag: Staratagene amp reference 7/30/02


title: Expt189 MCM256 vs W6G 3

description:
Simple annotation: Genome comparison, Map Recombination Event
Image: http://smd.stanford.edu/MicroArray/gifs/2005-10/64608.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1060, 27760; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Michael,,Miller Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.37; Parameter Scan Time = 13:09:28; Parameter PMT Volts = 520; Parameter Scan Date = 2005-10-16; Parameter Pixel Size = 10; Parameter Laser on time = 55300; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 2.54; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.3.1; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.37; Parameter Scan Time = 13:09:28; Parameter PMT Volts = 740; Parameter Scan Date = 2005-10-16; Parameter Pixel Size = 10; Parameter Laser on time = 201225; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 1.37; Parameter Scanning software version = 2.0.3.1; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller


title: Expt189 MCM252 vs W6G 3

description:
Simple annotation: Genome comparison, Map Recombination Event
Image: http://smd.stanford.edu/MicroArray/gifs/2005-10/64605.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1120, 27600; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Michael,,Miller Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.06; Parameter Scan Time = 12:40:38; Parameter PMT Volts = 490; Parameter Scan Date = 2005-10-16; Parameter Pixel Size = 10; Parameter Laser on time = 55271; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 2.58; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.3.1; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.06; Parameter Scan Time = 12:40:38; Parameter PMT Volts = 750; Parameter Scan Date = 2005-10-16; Parameter Pixel Size = 10; Parameter Laser on time = 201196; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 1.37; Parameter Scanning software version = 2.0.3.1; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller


title: HKCO40T

description:
Colon tissue samples for DNA microarrays; Samples of tumor and normal colon mucosa were collected from colon cancer resection from Department of Surgery, Queen Mary Hospital University of Hong Kong. Tissue was frozen in liquid nitrogen within 30 min of resection. Nonneoplastic mucosa from colon was dissected free of muscle and histologically confirmed to be tumor free by frozen section. Total RNA was extracted using Trizol (Invitrogen, Carlsbad, CA) from each tissue sample and processed for microarray hybridization.; Protocol Type = Biological Sample; Performer: Xin,,Chen
Simple annotation: Primary tumor, Colon Cancer
Image: http://smd.stanford.edu/MicroArray/gifs/2003-11/46393.gif
Disease State: Tumor Colon Samples

scan_protocol: Array images were scanned by using an Axon Scanner 4000B, and data were analyzed by using GenePix 3.0 (Axon Instruments, Union City, CA). Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1160, 5480; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Xin,,Chen Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.67; Parameter Scan Time = 00:26:38; Parameter PMT Volts = 590; Parameter Scan Date = 2003-10-22; Parameter Pixel Size = 10; Parameter Laser on time = 129710; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 3.20; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Xin,,Chen Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.67; Parameter Scan Time = 00:26:38; Parameter PMT Volts = 680; Parameter Scan Date = 2003-10-22; Parameter Pixel Size = 10; Parameter Laser on time = 348681; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.96; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Xin,,Chen

source_name: amplified, HKCO40T

tag: amplified, HKCO40T


title: HKCO06T

description:
Colon tissue samples for DNA microarrays; Samples of tumor and normal colon mucosa were collected from colon cancer resection from Department of Surgery, Queen Mary Hospital University of Hong Kong. Tissue was frozen in liquid nitrogen within 30 min of resection. Nonneoplastic mucosa from colon was dissected free of muscle and histologically confirmed to be tumor free by frozen section. Total RNA was extracted using Trizol (Invitrogen, Carlsbad, CA) from each tissue sample and processed for microarray hybridization.; Protocol Type = Biological Sample; Performer: Xin,,Chen
Simple annotation: Primary tumor, Colon Cancer
Image: http://smd.stanford.edu/MicroArray/gifs/2003-11/46630.gif
Disease State: Tumor Colon Samples

scan_protocol: Array images were scanned by using an Axon Scanner 4000B, and data were analyzed by using GenePix 3.0 (Axon Instruments, Union City, CA). Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 870, 4820; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Xin,,Chen Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.48; Parameter Scan Time = 00:03:34; Parameter PMT Volts = 620; Parameter Scan Date = 2003-11-12; Parameter Pixel Size = 10; Parameter Laser on time = 134557; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 3.27; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Xin,,Chen Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.48; Parameter Scan Time = 00:03:34; Parameter PMT Volts = 650; Parameter Scan Date = 2003-11-12; Parameter Pixel Size = 10; Parameter Laser on time = 353527; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.94; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Xin,,Chen

source_name: amplified HKCO06T

tag: amplified HKCO06T


title: HKCO54T

description:
Colon tissue samples for DNA microarrays; Samples of tumor and normal colon mucosa were collected from colon cancer resection from Department of Surgery, Queen Mary Hospital University of Hong Kong. Tissue was frozen in liquid nitrogen within 30 min of resection. Nonneoplastic mucosa from colon was dissected free of muscle and histologically confirmed to be tumor free by frozen section. Total RNA was extracted using Trizol (Invitrogen, Carlsbad, CA) from each tissue sample and processed for microarray hybridization.; Protocol Type = Biological Sample; Performer: Xin,,Chen
Simple annotation: Primary tumor, Colon Cancer
Image: http://smd.stanford.edu/MicroArray/gifs/2003-11/46717.gif
Disease State: Tumor Colon Samples

scan_protocol: Array images were scanned by using an Axon Scanner 4000B, and data were analyzed by using GenePix 3.0 (Axon Instruments, Union City, CA). Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1360, 5560; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Xin,,Chen Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 30.75; Parameter Scan Time = 21:48:17; Parameter PMT Volts = 610; Parameter Scan Date = 2003-11-13; Parameter Pixel Size = 10; Parameter Laser on time = 135112; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 3.90; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Xin,,Chen Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 30.75; Parameter Scan Time = 21:48:17; Parameter PMT Volts = 650; Parameter Scan Date = 2003-11-13; Parameter Pixel Size = 10; Parameter Laser on time = 354082; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.92; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Xin,,Chen

source_name: amplified HKCO54T

tag: amplified HKCO54T


title: HKCO01N

description:
Colon tissue samples for DNA microarrays; Samples of tumor and normal colon mucosa were collected from colon cancer resection from Department of Surgery, Queen Mary Hospital University of Hong Kong. Tissue was frozen in liquid nitrogen within 30 min of resection. Nonneoplastic mucosa from colon was dissected free of muscle and histologically confirmed to be tumor free by frozen section. Total RNA was extracted using Trizol (Invitrogen, Carlsbad, CA) from each tissue sample and processed for microarray hybridization.; Protocol Type = Biological Sample; Performer: Xin,,Chen
Simple annotation: Primary tumor, Colon Cancer
Image: http://smd.stanford.edu/MicroArray/gifs/2003-11/46626.gif
Disease State: Healthy Normal Colon sample

scan_protocol: Array images were scanned by using an Axon Scanner 4000B, and data were analyzed by using GenePix 3.0 (Axon Instruments, Union City, CA). Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 850, 5040; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Xin,,Chen Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.33; Parameter Scan Time = 23:05:58; Parameter PMT Volts = 620; Parameter Scan Date = 2003-11-11; Parameter Pixel Size = 10; Parameter Laser on time = 134499; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 3.25; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Xin,,Chen Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.33; Parameter Scan Time = 23:05:58; Parameter PMT Volts = 680; Parameter Scan Date = 2003-11-11; Parameter Pixel Size = 10; Parameter Laser on time = 353469; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.93; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Xin,,Chen

source_name: amplified HKCO01N

tag: amplified HKCO01N


title: HKCO51T

description:
Colon tissue samples for DNA microarrays; Samples of tumor and normal colon mucosa were collected from colon cancer resection from Department of Surgery, Queen Mary Hospital University of Hong Kong. Tissue was frozen in liquid nitrogen within 30 min of resection. Nonneoplastic mucosa from colon was dissected free of muscle and histologically confirmed to be tumor free by frozen section. Total RNA was extracted using Trizol (Invitrogen, Carlsbad, CA) from each tissue sample and processed for microarray hybridization.; Protocol Type = Biological Sample; Performer: Xin,,Chen
Simple annotation: Primary tumor, Colon Cancer
Image: http://smd.stanford.edu/MicroArray/gifs/2003-11/46680.gif
Disease State: Tumor Colon Samples

scan_protocol: Array images were scanned by using an Axon Scanner 4000B, and data were analyzed by using GenePix 3.0 (Axon Instruments, Union City, CA). Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1220, 5280; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Xin,,Chen Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.45; Parameter Scan Time = 23:29:43; Parameter PMT Volts = 620; Parameter Scan Date = 2003-11-12; Parameter Pixel Size = 10; Parameter Laser on time = 134888; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 3.42; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Xin,,Chen Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.45; Parameter Scan Time = 23:29:43; Parameter PMT Volts = 690; Parameter Scan Date = 2003-11-12; Parameter Pixel Size = 10; Parameter Laser on time = 353859; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.94; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Xin,,Chen

source_name: amplified HKCO51T

tag: amplified HKCO51T


title: HKCO52T

description:
Colon tissue samples for DNA microarrays; Samples of tumor and normal colon mucosa were collected from colon cancer resection from Department of Surgery, Queen Mary Hospital University of Hong Kong. Tissue was frozen in liquid nitrogen within 30 min of resection. Nonneoplastic mucosa from colon was dissected free of muscle and histologically confirmed to be tumor free by frozen section. Total RNA was extracted using Trizol (Invitrogen, Carlsbad, CA) from each tissue sample and processed for microarray hybridization.; Protocol Type = Biological Sample; Performer: Xin,,Chen
Simple annotation: Primary tumor, Colon Cancer
Image: http://smd.stanford.edu/MicroArray/gifs/2003-11/46682.gif
Disease State: Tumor Colon Samples

scan_protocol: Array images were scanned by using an Axon Scanner 4000B, and data were analyzed by using GenePix 3.0 (Axon Instruments, Union City, CA). Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1380, 5380; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Xin,,Chen Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.48; Parameter Scan Time = 23:51:53; Parameter PMT Volts = 620; Parameter Scan Date = 2003-11-12; Parameter Pixel Size = 10; Parameter Laser on time = 134911; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 3.40; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Xin,,Chen Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.48; Parameter Scan Time = 23:51:53; Parameter PMT Volts = 690; Parameter Scan Date = 2003-11-12; Parameter Pixel Size = 10; Parameter Laser on time = 353881; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.93; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Xin,,Chen

source_name: amplified HKCO52T

tag: amplified HKCO52T


title: HKCO35T

description:
Colon tissue samples for DNA microarrays; Samples of tumor and normal colon mucosa were collected from colon cancer resection from Department of Surgery, Queen Mary Hospital University of Hong Kong. Tissue was frozen in liquid nitrogen within 30 min of resection. Nonneoplastic mucosa from colon was dissected free of muscle and histologically confirmed to be tumor free by frozen section. Total RNA was extracted using Trizol (Invitrogen, Carlsbad, CA) from each tissue sample and processed for microarray hybridization.; Protocol Type = Biological Sample; Performer: Xin,,Chen
Simple annotation: Primary tumor, Colon Cancer
Image: http://smd.stanford.edu/MicroArray/gifs/2003-11/46545.gif
Disease State: Tumor Colon Samples

scan_protocol: Array images were scanned by using an Axon Scanner 4000B, and data were analyzed by using GenePix 3.0 (Axon Instruments, Union City, CA). Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1120, 5200; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Xin,,Chen Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.21; Parameter Scan Time = 23:02:12; Parameter PMT Volts = 640; Parameter Scan Date = 2003-11-07; Parameter Pixel Size = 10; Parameter Laser on time = 133868; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 3.37; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Xin,,Chen Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.21; Parameter Scan Time = 23:02:12; Parameter PMT Volts = 650; Parameter Scan Date = 2003-11-07; Parameter Pixel Size = 10; Parameter Laser on time = 352838; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.93; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Xin,,Chen

source_name: amplified, HKCO35T

tag: amplified, HKCO35T


title: Expt189 MCM256 vs W6G 2rr

description:
Simple annotation: Genome comparison, Map Recombination Event
Image: http://smd.stanford.edu/MicroArray/gifs/2005-10/64524.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 530, 27470; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Michael,,Miller Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.31; Parameter Scan Time = 09:53:21; Parameter PMT Volts = 600; Parameter Scan Date = 2005-10-13; Parameter Pixel Size = 10; Parameter Laser on time = 54940; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 2.21; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.3.1; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.31; Parameter Scan Time = 09:53:21; Parameter PMT Volts = 720; Parameter Scan Date = 2005-10-13; Parameter Pixel Size = 10; Parameter Laser on time = 200864; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 1.33; Parameter Scanning software version = 2.0.3.1; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller


title: Expt189 MCM252 vs W6G 2rr

description:
Simple annotation: Genome comparison, Map Recombination Event
Image: http://smd.stanford.edu/MicroArray/gifs/2005-10/64521.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 530, 27340; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Michael,,Miller Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.16; Parameter Scan Time = 09:35:41; Parameter PMT Volts = 560; Parameter Scan Date = 2005-10-13; Parameter Pixel Size = 10; Parameter Laser on time = 54922; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 2.20; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.3.1; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.16; Parameter Scan Time = 09:35:41; Parameter PMT Volts = 710; Parameter Scan Date = 2005-10-13; Parameter Pixel Size = 10; Parameter Laser on time = 200847; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 1.34; Parameter Scanning software version = 2.0.3.1; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller


title: Expt189 MCM260 vs W6G 2rr

description:
Simple annotation: Genome comparison, Map Recombination Event
Image: http://smd.stanford.edu/MicroArray/gifs/2005-10/64528.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 590, 27750; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Michael,,Miller Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.59; Parameter Scan Time = 10:15:56; Parameter PMT Volts = 590; Parameter Scan Date = 2005-10-13; Parameter Pixel Size = 10; Parameter Laser on time = 54962; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 2.22; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.3.1; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.59; Parameter Scan Time = 10:15:56; Parameter PMT Volts = 740; Parameter Scan Date = 2005-10-13; Parameter Pixel Size = 10; Parameter Laser on time = 200887; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 1.34; Parameter Scanning software version = 2.0.3.1; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller


title: Expt189 MCM263 vs W6G 4rr

description:
Simple annotation: Genome comparison, Map Recombination Event
Image: http://smd.stanford.edu/MicroArray/gifs/2005-10/64643.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 160, 27310; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Michael,,Miller Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.62; Parameter Scan Time = 09:55:09; Parameter PMT Volts = 580; Parameter Scan Date = 2005-10-20; Parameter Pixel Size = 10; Parameter Laser on time = 55880; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 2.26; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.3.1; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.62; Parameter Scan Time = 09:55:09; Parameter PMT Volts = 760; Parameter Scan Date = 2005-10-20; Parameter Pixel Size = 10; Parameter Laser on time = 201805; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 1.35; Parameter Scanning software version = 2.0.3.1; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller


title: Expt189 MCM262 vs W6G 2rr

description:
Simple annotation: Genome comparison, Map Recombination Event
Image: http://smd.stanford.edu/MicroArray/gifs/2005-10/64529.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 590, 27750; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Michael,,Miller Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.72; Parameter Scan Time = 10:28:29; Parameter PMT Volts = 590; Parameter Scan Date = 2005-10-13; Parameter Pixel Size = 10; Parameter Laser on time = 54975; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 2.24; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.3.1; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.72; Parameter Scan Time = 10:28:29; Parameter PMT Volts = 730; Parameter Scan Date = 2005-10-13; Parameter Pixel Size = 10; Parameter Laser on time = 200900; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 1.35; Parameter Scanning software version = 2.0.3.1; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller


title: Expt189 MCM256 vs W6G 1

description:
Simple annotation: Genome comparison, Map Recombination Event
Image: http://smd.stanford.edu/MicroArray/gifs/2005-10/64512.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 590, 27010; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Michael,,Miller Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.33; Parameter Scan Time = 09:38:06; Parameter PMT Volts = 550; Parameter Scan Date = 2005-10-11; Parameter Pixel Size = 10; Parameter Laser on time = 54804; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 2.26; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.3.1; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.33; Parameter Scan Time = 09:38:06; Parameter PMT Volts = 700; Parameter Scan Date = 2005-10-11; Parameter Pixel Size = 10; Parameter Laser on time = 200729; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 1.33; Parameter Scanning software version = 2.0.3.1; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller


title: Expt189 MCM263 vs W6G 3rr

description:
Simple annotation: Genome comparison, Map Recombination Event
Image: http://smd.stanford.edu/MicroArray/gifs/2005-10/64642.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 180, 27860; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Michael,,Miller Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.45; Parameter Scan Time = 09:41:26; Parameter PMT Volts = 570; Parameter Scan Date = 2005-10-20; Parameter Pixel Size = 10; Parameter Laser on time = 55866; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 2.25; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.3.1; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.45; Parameter Scan Time = 09:41:26; Parameter PMT Volts = 750; Parameter Scan Date = 2005-10-20; Parameter Pixel Size = 10; Parameter Laser on time = 201791; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 1.34; Parameter Scanning software version = 2.0.3.1; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller


title: Expt189 MCM262 vs W6G 4rr

description:
Simple annotation: Genome comparison, Map Recombination Event
Image: http://smd.stanford.edu/MicroArray/gifs/2005-10/64641.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 390, 28230; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Michael,,Miller Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.39; Parameter Scan Time = 09:35:58; Parameter PMT Volts = 570; Parameter Scan Date = 2005-10-20; Parameter Pixel Size = 10; Parameter Laser on time = 55860; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 2.25; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.3.1; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.39; Parameter Scan Time = 09:35:58; Parameter PMT Volts = 740; Parameter Scan Date = 2005-10-20; Parameter Pixel Size = 10; Parameter Laser on time = 201785; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 1.34; Parameter Scanning software version = 2.0.3.1; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller


title: Expt189 MCM254 vs W6G 2rr

description:
Simple annotation: Genome comparison, Map Recombination Event
Image: http://smd.stanford.edu/MicroArray/gifs/2005-10/64523.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 530, 28040; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Michael,,Miller Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.26; Parameter Scan Time = 09:46:59; Parameter PMT Volts = 580; Parameter Scan Date = 2005-10-13; Parameter Pixel Size = 10; Parameter Laser on time = 54934; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 2.20; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.3.1; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.26; Parameter Scan Time = 09:46:59; Parameter PMT Volts = 710; Parameter Scan Date = 2005-10-13; Parameter Pixel Size = 10; Parameter Laser on time = 200859; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 1.32; Parameter Scanning software version = 2.0.3.1; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller


title: Expt189 MCM259 vs W6G 2rr

description:
Simple annotation: Genome comparison, Map Recombination Event
Image: http://smd.stanford.edu/MicroArray/gifs/2005-10/64526.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 590, 27750; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Michael,,Miller Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.46; Parameter Scan Time = 10:05:37; Parameter PMT Volts = 590; Parameter Scan Date = 2005-10-13; Parameter Pixel Size = 10; Parameter Laser on time = 54952; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 2.21; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.3.1; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.46; Parameter Scan Time = 10:05:37; Parameter PMT Volts = 730; Parameter Scan Date = 2005-10-13; Parameter Pixel Size = 10; Parameter Laser on time = 200877; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 1.34; Parameter Scanning software version = 2.0.3.1; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller


title: Expt189 MCM262 vs W6G 1

description:
Simple annotation: Genome comparison, Map Recombination Event
Image: http://smd.stanford.edu/MicroArray/gifs/2005-10/64517.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 520, 27370; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Michael,,Miller Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.68; Parameter Scan Time = 10:06:30; Parameter PMT Volts = 560; Parameter Scan Date = 2005-10-11; Parameter Pixel Size = 10; Parameter Laser on time = 54833; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 2.32; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.3.1; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.68; Parameter Scan Time = 10:06:30; Parameter PMT Volts = 710; Parameter Scan Date = 2005-10-11; Parameter Pixel Size = 10; Parameter Laser on time = 200758; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 1.35; Parameter Scanning software version = 2.0.3.1; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller


title: Expt189 MCM254 vs W6G 1

description:
Simple annotation: Genome comparison, Map Recombination Event
Image: http://smd.stanford.edu/MicroArray/gifs/2005-10/64511.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 550, 26150; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Michael,,Miller Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.26; Parameter Scan Time = 09:28:25; Parameter PMT Volts = 570; Parameter Scan Date = 2005-10-11; Parameter Pixel Size = 10; Parameter Laser on time = 54794; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 2.24; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.3.1; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.26; Parameter Scan Time = 09:28:25; Parameter PMT Volts = 720; Parameter Scan Date = 2005-10-11; Parameter Pixel Size = 10; Parameter Laser on time = 200719; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 1.32; Parameter Scanning software version = 2.0.3.1; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller


title: Expt189 MCM260 vs W6G 1

description:
Simple annotation: Genome comparison, Map Recombination Event
Image: http://smd.stanford.edu/MicroArray/gifs/2005-10/64515.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 540, 27430; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Michael,,Miller Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.52; Parameter Scan Time = 09:54:51; Parameter PMT Volts = 560; Parameter Scan Date = 2005-10-11; Parameter Pixel Size = 10; Parameter Laser on time = 54821; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 2.31; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.3.1; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.52; Parameter Scan Time = 09:54:51; Parameter PMT Volts = 710; Parameter Scan Date = 2005-10-11; Parameter Pixel Size = 10; Parameter Laser on time = 200746; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 1.34; Parameter Scanning software version = 2.0.3.1; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller


title: Expt189 MCM257 vs W6G 4rr

description:
Simple annotation: Genome comparison, Map Recombination Event
Image: http://smd.stanford.edu/MicroArray/gifs/2005-10/64638.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 120, 27830; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Michael,,Miller Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.69; Parameter Scan Time = 10:05:55; Parameter PMT Volts = 580; Parameter Scan Date = 2005-10-20; Parameter Pixel Size = 10; Parameter Laser on time = 55891; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 2.28; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.3.1; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.69; Parameter Scan Time = 10:05:55; Parameter PMT Volts = 750; Parameter Scan Date = 2005-10-20; Parameter Pixel Size = 10; Parameter Laser on time = 201816; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 1.35; Parameter Scanning software version = 2.0.3.1; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller


title: Expt189 MCM257 vs W6G 1

description:
Simple annotation: Genome comparison, Map Recombination Event
Image: http://smd.stanford.edu/MicroArray/gifs/2005-10/64513.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 590, 27010; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Michael,,Miller Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.41; Parameter Scan Time = 09:43:15; Parameter PMT Volts = 550; Parameter Scan Date = 2005-10-11; Parameter Pixel Size = 10; Parameter Laser on time = 54810; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 2.28; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.3.1; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.41; Parameter Scan Time = 09:43:15; Parameter PMT Volts = 700; Parameter Scan Date = 2005-10-11; Parameter Pixel Size = 10; Parameter Laser on time = 200735; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 1.33; Parameter Scanning software version = 2.0.3.1; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller


title: Expt189 MCM253 vs W6G 1

description:
Simple annotation: Genome comparison, Map Recombination Event
Image: http://smd.stanford.edu/MicroArray/gifs/2005-10/64510.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 380, 26750; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Michael,,Miller Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.17; Parameter Scan Time = 09:21:34; Parameter PMT Volts = 570; Parameter Scan Date = 2005-10-11; Parameter Pixel Size = 10; Parameter Laser on time = 54788; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 2.25; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.3.1; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.17; Parameter Scan Time = 09:21:34; Parameter PMT Volts = 720; Parameter Scan Date = 2005-10-11; Parameter Pixel Size = 10; Parameter Laser on time = 200713; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 1.32; Parameter Scanning software version = 2.0.3.1; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller


title: Expt189 MCM257 vs W6G 2rr

description:
Simple annotation: Genome comparison, Map Recombination Event
Image: http://smd.stanford.edu/MicroArray/gifs/2005-10/64525.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 620, 28010; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Michael,,Miller Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.41; Parameter Scan Time = 09:59:39; Parameter PMT Volts = 590; Parameter Scan Date = 2005-10-13; Parameter Pixel Size = 10; Parameter Laser on time = 54946; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 2.20; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.3.1; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.41; Parameter Scan Time = 09:59:39; Parameter PMT Volts = 730; Parameter Scan Date = 2005-10-13; Parameter Pixel Size = 10; Parameter Laser on time = 200871; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 1.34; Parameter Scanning software version = 2.0.3.1; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller


title: Expt189 MCM253 vs W6G 2rr

description:
Simple annotation: Genome comparison, Map Recombination Event
Image: http://smd.stanford.edu/MicroArray/gifs/2005-10/64522.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 530, 27340; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Michael,,Miller Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.21; Parameter Scan Time = 09:42:00; Parameter PMT Volts = 560; Parameter Scan Date = 2005-10-13; Parameter Pixel Size = 10; Parameter Laser on time = 54928; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 2.20; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.3.1; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.21; Parameter Scan Time = 09:42:00; Parameter PMT Volts = 710; Parameter Scan Date = 2005-10-13; Parameter Pixel Size = 10; Parameter Laser on time = 200853; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 1.33; Parameter Scanning software version = 2.0.3.1; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller


title: Expt189 MCM252 vs W6G 1

description:
Simple annotation: Genome comparison, Map Recombination Event
Image: http://smd.stanford.edu/MicroArray/gifs/2005-10/64509.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 400, 26400; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Michael,,Miller Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.07; Parameter Scan Time = 09:11:54; Parameter PMT Volts = 560; Parameter Scan Date = 2005-10-11; Parameter Pixel Size = 10; Parameter Laser on time = 54779; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 2.19; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.3.1; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.07; Parameter Scan Time = 09:11:54; Parameter PMT Volts = 720; Parameter Scan Date = 2005-10-11; Parameter Pixel Size = 10; Parameter Laser on time = 200704; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 1.32; Parameter Scanning software version = 2.0.3.1; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller


title: Expt189 MCM259 vs W6G 1

description:
Simple annotation: Genome comparison, Map Recombination Event
Image: http://smd.stanford.edu/MicroArray/gifs/2005-10/64527.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 590, 27750; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Michael,,Miller Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.52; Parameter Scan Time = 10:11:01; Parameter PMT Volts = 590; Parameter Scan Date = 2005-10-13; Parameter Pixel Size = 10; Parameter Laser on time = 54957; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 2.22; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.3.1; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.52; Parameter Scan Time = 10:11:01; Parameter PMT Volts = 740; Parameter Scan Date = 2005-10-13; Parameter Pixel Size = 10; Parameter Laser on time = 200882; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 1.34; Parameter Scanning software version = 2.0.3.1; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller


title: Expt189 MCM253 vs W6G 4rr

description:
Simple annotation: Genome comparison, Map Recombination Event
Image: http://smd.stanford.edu/MicroArray/gifs/2005-10/64635.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1220, 27330; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Michael,,Miller Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.18; Parameter Scan Time = 09:04:14; Parameter PMT Volts = 570; Parameter Scan Date = 2005-10-20; Parameter Pixel Size = 10; Parameter Laser on time = 55828; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 2.23; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.3.1; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.18; Parameter Scan Time = 09:04:14; Parameter PMT Volts = 730; Parameter Scan Date = 2005-10-20; Parameter Pixel Size = 10; Parameter Laser on time = 201753; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 1.32; Parameter Scanning software version = 2.0.3.1; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller


title: Expt189 MCM259 vs W6G 4rr

description:
Simple annotation: Genome comparison, Map Recombination Event
Image: http://smd.stanford.edu/MicroArray/gifs/2005-10/64639.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 50, 28230; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Michael,,Miller Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.31; Parameter Scan Time = 09:21:42; Parameter PMT Volts = 570; Parameter Scan Date = 2005-10-20; Parameter Pixel Size = 10; Parameter Laser on time = 55846; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 2.24; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.3.1; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.31; Parameter Scan Time = 09:21:42; Parameter PMT Volts = 740; Parameter Scan Date = 2005-10-20; Parameter Pixel Size = 10; Parameter Laser on time = 201771; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 1.34; Parameter Scanning software version = 2.0.3.1; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller


title: Expt189 MCM252 vs W6G 4rr

description:
Simple annotation: Genome comparison, Map Recombination Event
Image: http://smd.stanford.edu/MicroArray/gifs/2005-10/64633.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1320, 27520; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Michael,,Miller Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 39.98; Parameter Scan Time = 08:51:40; Parameter PMT Volts = 550; Parameter Scan Date = 2005-10-20; Parameter Pixel Size = 10; Parameter Laser on time = 55816; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 2.12; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.3.1; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 39.98; Parameter Scan Time = 08:51:40; Parameter PMT Volts = 720; Parameter Scan Date = 2005-10-20; Parameter Pixel Size = 10; Parameter Laser on time = 201741; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 1.33; Parameter Scanning software version = 2.0.3.1; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller


title: Expt189 MCM256 vs W6G 4rr

description:
Simple annotation: Genome comparison, Map Recombination Event
Image: http://smd.stanford.edu/MicroArray/gifs/2005-10/64637.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1210, 27090; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Michael,,Miller Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.23; Parameter Scan Time = 09:16:02; Parameter PMT Volts = 570; Parameter Scan Date = 2005-10-20; Parameter Pixel Size = 10; Parameter Laser on time = 55841; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 2.23; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.3.1; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.23; Parameter Scan Time = 09:16:02; Parameter PMT Volts = 740; Parameter Scan Date = 2005-10-20; Parameter Pixel Size = 10; Parameter Laser on time = 201766; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 1.33; Parameter Scanning software version = 2.0.3.1; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller


title: Expt189 MCM260 vs W6G 4rr

description:
Simple annotation: Genome comparison, Map Recombination Event
Image: http://smd.stanford.edu/MicroArray/gifs/2005-10/64640.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 390, 28230; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Michael,,Miller Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.37; Parameter Scan Time = 09:27:24; Parameter PMT Volts = 570; Parameter Scan Date = 2005-10-20; Parameter Pixel Size = 10; Parameter Laser on time = 55852; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 2.25; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.3.1; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.37; Parameter Scan Time = 09:27:24; Parameter PMT Volts = 740; Parameter Scan Date = 2005-10-20; Parameter Pixel Size = 10; Parameter Laser on time = 201777; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 1.34; Parameter Scanning software version = 2.0.3.1; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller


title: Expt189 MCM254 vs W6G 4rr

description:
Simple annotation: Genome comparison, Map Recombination Event
Image: http://smd.stanford.edu/MicroArray/gifs/2005-10/64636.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1170, 27510; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Michael,,Miller Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.22; Parameter Scan Time = 09:10:32; Parameter PMT Volts = 570; Parameter Scan Date = 2005-10-20; Parameter Pixel Size = 10; Parameter Laser on time = 55835; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 2.24; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.3.1; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.22; Parameter Scan Time = 09:10:32; Parameter PMT Volts = 730; Parameter Scan Date = 2005-10-20; Parameter Pixel Size = 10; Parameter Laser on time = 201760; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 1.34; Parameter Scanning software version = 2.0.3.1; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller


title: Expt189 MCM253 vs W6G 5

description:
Simple annotation: Genome comparison, Map Recombination Event
Image: http://smd.stanford.edu/MicroArray/gifs/2005-10/64634.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 990, 27750; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Michael,,Miller Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.08; Parameter Scan Time = 08:58:04; Parameter PMT Volts = 550; Parameter Scan Date = 2005-10-20; Parameter Pixel Size = 10; Parameter Laser on time = 55823; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 2.23; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.3.1; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 40.08; Parameter Scan Time = 08:58:04; Parameter PMT Volts = 720; Parameter Scan Date = 2005-10-20; Parameter Pixel Size = 10; Parameter Laser on time = 201748; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000A [48444]; Parameter Laser Power = 1.33; Parameter Scanning software version = 2.0.3.1; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Michael,,Miller


title: 4wk B w1

status: Public on Aug 15 2007

description:
Simple annotation: Mutant vs Wild-type, wild-type
Image: http://smd.stanford.edu/MicroArray/gifs/2004-07/54276.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1640, 5120; Parameter Datafile type = GenePix Results 1.4; Software: type: feature extraction; Performer: Charandle,,Jordan Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.39; Parameter Scan Time = 08:13:52; Parameter PMT Volts = 540; Parameter Scan Date = 2004-07-29; Parameter Pixel Size = 10; Parameter Laser on time = 183970; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.44; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Charandle,,Jordan Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.39; Parameter Scan Time = 08:13:52; Parameter PMT Volts = 620; Parameter Scan Date = 2004-07-29; Parameter Pixel Size = 10; Parameter Laser on time = 402939; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.67; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Charandle,,Jordan

organism: Mus musculus

source_name: ref

label_protocol:
not provided
Extract preparation - Channel 1; Methods by which RNA/DNA/protein is prepared from a biological sample prior to amplification or labeling for channel 1 (green); Protocol Type = Extract preparation; Parameter Channel 1 reference name = pooled mouse cerebellum RNA; Performer: Charandle,,Jordan
Labeling - Channel 1; Methods for labeling extracted molecules that are used in hybridizations and scanned in Channel 1 (green).; Protocol Type = Labeling; Parameter Name = Cyscribe First Strand Labeling Kit (Amersham Biosciences); Performer: Charandle,,Jordan

tag: ref

source_name: wt

label_protocol:
not provided
Growth Conditions; Parameters that describe the conditions under which the biological sample was grown or propagated; Protocol Type = Growth Conditions; Parameter Notebook page = Notebooks #5-6 pages 1-200; Performer: Charandle,,Jordan
Biological Sample - Channel 2; Characteristics of the original biological sample used to generate the extract that was labeled and scanned in channel 2 (red).; Protocol Type = Biological Sample; Parameter Cell Type = Prepared from Cerebellum; Performer: Charandle,,Jordan
Extract preparation - Channel 2; Methods by which RNA/DNA/protein is prepared from a biological sample prior to amplification or labeling for channel 2 (red).; Protocol Type = Extract preparation; Parameter Date prepared = 2004-2005; Parameter Channel 2 reference name = wild-type cerebellum cDNA; Parameter Extraction method = Trizol Reagent (Invitrogen/Gibco); Performer: Charandle,,Jordan
Labeling - Channel 2; Methods for labeling extracted molecules that are used in hybridizations and scanned in Channel 2 (red); Protocol Type = Labeling; Parameter Name = Cyscribe First Strand Labeling Kit (Amersham Biosciences); Performer: Charandle,,Jordan

tag: wt


title: 8wk B w6

description:
Simple annotation: Mutant vs Wild-type, wild-type
Image: http://smd.stanford.edu/MicroArray/gifs/2004-06/52761.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1440, 4880; Parameter Datafile type = GenePix Results 1.4; Software: type: feature extraction; Performer: Charandle,,Jordan Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.88; Parameter Scan Time = 01:51:06; Parameter PMT Volts = 530; Parameter Scan Date = 2004-05-28; Parameter Pixel Size = 10; Parameter Laser on time = 170598; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 3.20; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Charandle,,Jordan Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.88; Parameter Scan Time = 01:51:06; Parameter PMT Volts = 680; Parameter Scan Date = 2004-05-28; Parameter Pixel Size = 10; Parameter Laser on time = 389569; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.72; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Charandle,,Jordan

source_name: reference

tag: reference


title: 4wk B w6

description:
Simple annotation: Mutant vs Wild-type, wild-type
Image: http://smd.stanford.edu/MicroArray/gifs/2004-06/52763.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1440, 4720; Parameter Datafile type = GenePix Results 1.4; Software: type: feature extraction; Performer: Charandle,,Jordan Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 32.31; Parameter Scan Time = 03:03:19; Parameter PMT Volts = 540; Parameter Scan Date = 2004-05-28; Parameter Pixel Size = 10; Parameter Laser on time = 170670; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.48; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Charandle,,Jordan Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 32.31; Parameter Scan Time = 03:03:19; Parameter PMT Volts = 660; Parameter Scan Date = 2004-05-28; Parameter Pixel Size = 10; Parameter Laser on time = 389640; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.70; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Charandle,,Jordan


title: 8wk J w2

description:
Simple annotation: Mutant vs Wild-type, wild-type
Image: http://smd.stanford.edu/MicroArray/gifs/2004-09/55321.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1160, 4800; Parameter Datafile type = GenePix Results 1.4; Software: type: feature extraction; Performer: Charandle,,Jordan Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.91; Parameter Scan Time = 01:35:36; Parameter PMT Volts = 540; Parameter Scan Date = 2004-09-03; Parameter Pixel Size = 10; Parameter Laser on time = 190157; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.47; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Charandle,,Jordan Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.91; Parameter Scan Time = 01:35:36; Parameter PMT Volts = 590; Parameter Scan Date = 2004-09-03; Parameter Pixel Size = 10; Parameter Laser on time = 409125; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.68; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Charandle,,Jordan


title: 4wk B m6

description:
Simple annotation: Mutant vs Wild-type, Mutant
Image: http://smd.stanford.edu/MicroArray/gifs/2004-06/52762.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1440, 4720; Parameter Datafile type = GenePix Results 1.4; Software: type: feature extraction; Performer: Charandle,,Jordan Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 32.00; Parameter Scan Time = 02:00:20; Parameter PMT Volts = 530; Parameter Scan Date = 2004-05-28; Parameter Pixel Size = 10; Parameter Laser on time = 170607; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.76; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Charandle,,Jordan Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 32.00; Parameter Scan Time = 02:00:20; Parameter PMT Volts = 680; Parameter Scan Date = 2004-05-28; Parameter Pixel Size = 10; Parameter Laser on time = 389578; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.70; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Charandle,,Jordan

source_name: mut

label_protocol:
not provided
Growth Conditions; Parameters that describe the conditions under which the biological sample was grown or propagated; Protocol Type = Growth Conditions; Parameter Notebook page = Notebooks #5-6 pages 1-200; Performer: Charandle,,Jordan
Biological Sample - Channel 2; Characteristics of the original biological sample used to generate the extract that was labeled and scanned in channel 2 (red).; Protocol Type = Biological Sample; Parameter Cell Type = Prepared from Cerebellum; Performer: Charandle,,Jordan
Extract preparation - Channel 2; Methods by which RNA/DNA/protein is prepared from a biological sample prior to amplification or labeling for channel 2 (red).; Protocol Type = Extract preparation; Parameter Date prepared = 2004-2005; Parameter Channel 2 reference name = mutant cerebellum cDNA; Parameter Extraction method = Trizol Reagent (Invitrogen/Gibco); Performer: Charandle,,Jordan
Labeling - Channel 2; Methods for labeling extracted molecules that are used in hybridizations and scanned in Channel 2 (red); Protocol Type = Labeling; Parameter Name = Cyscribe First Strand Labeling Kit (Amersham Biosciences); Performer: Charandle,,Jordan

tag: mut


title: 4wk B w2

description:
Simple annotation: Mutant vs Wild-type, wild-type
Image: http://smd.stanford.edu/MicroArray/gifs/2004-07/54274.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1640, 5120; Parameter Datafile type = GenePix Results 1.4; Software: type: feature extraction; Performer: Charandle,,Jordan Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.82; Parameter Scan Time = 08:40:06; Parameter PMT Volts = 580; Parameter Scan Date = 2004-07-29; Parameter Pixel Size = 10; Parameter Laser on time = 183997; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.88; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Charandle,,Jordan Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.82; Parameter Scan Time = 08:40:06; Parameter PMT Volts = 620; Parameter Scan Date = 2004-07-29; Parameter Pixel Size = 10; Parameter Laser on time = 402965; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.68; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Charandle,,Jordan


title: 8wk B w3

description:
Simple annotation: Mutant vs Wild-type, wild-type
Image: http://smd.stanford.edu/MicroArray/gifs/2004-06/52755.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1600, 4960; Parameter Datafile type = GenePix Results 1.4; Software: type: feature extraction; Performer: Charandle,,Jordan Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.00; Parameter Scan Time = 01:39:36; Parameter PMT Volts = 520; Parameter Scan Date = 2004-05-15; Parameter Pixel Size = 10; Parameter Laser on time = 167951; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 3.09; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Charandle,,Jordan Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.00; Parameter Scan Time = 01:39:36; Parameter PMT Volts = 720; Parameter Scan Date = 2004-05-15; Parameter Pixel Size = 10; Parameter Laser on time = 386921; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.68; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Charandle,,Jordan


title: 8wk B m5

description:
Simple annotation: Mutant vs Wild-type, Mutant
Image: http://smd.stanford.edu/MicroArray/gifs/2004-06/52758.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1440, 4720; Parameter Datafile type = GenePix Results 1.4; Software: type: feature extraction; Performer: Charandle,,Jordan Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 32.16; Parameter Scan Time = 02:44:24; Parameter PMT Volts = 580; Parameter Scan Date = 2004-05-28; Parameter Pixel Size = 10; Parameter Laser on time = 170651; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 3.09; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Charandle,,Jordan Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 32.16; Parameter Scan Time = 02:44:24; Parameter PMT Volts = 660; Parameter Scan Date = 2004-05-28; Parameter Pixel Size = 10; Parameter Laser on time = 389621; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.71; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Charandle,,Jordan