title: PCR_ovoid_1_3

description: amplification mode=PCR  biological material=ovoid embryo  target replicate=1  array replicate=3.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: PCR_tubular_1_3

status: Public on Dec 20 2007

type: RNA

city: Jouy en Josas

first: Isabelle

last: Hue

address: Domaine de Vilvert

department: PHASE

country: France

institute: INRA

description: amplification mode=PCR  biological material=tubular embryo  target replicate=1  array replicate=3.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.

hyb_protocol: Prehybridisation in ExpressHybTM Hybridization Solution (Clontech) at 68°C during 1h (14ml). Hybridisation using new ExpressHybTM Hybridization Solution (Clontech) at 68°C overnight (10ml). Arrays were washed four times in 2X SSC, 1% SDS and once in 0.1X SSC, 0.5% SDS at 68°C for 30 min each. They were then exposed to phosphor-screens (Amersham) for 7 days.

scan_protocol: Scanner: STORM 760 from Molecular Dynamics. Raw data set : Feature extraction with Imagene 5.1 software from BioDiscovery (Proteigene)

data_processing: Intensity: Signal Mean, Normalization: Signal mean were log2 transformed and normalized by the mean of the all intensities on the array. For the statistical analyses described on the paper, the mean of the 4 array_replicates was done for each target_replicate and the data was standardized by gene. Furthermore, for the valid spots analysis, we consider the Flag column from Imagene 5.1 Software (flag=0 corresponds to a valid signal compare to his own local background whereas flag=2 corresponds to an non valid signal, ie: background>Signal Mean)

molecule: total RNA

organism: Bos taurus

source_name: Embryonic tissues immediately snap-frozen. Total RNA extracted with RNA-PlusTM (QBioGene). RNA quality verified by intact ribosomal bands on a 1% agarose gel (28S and 18S) and A260/280 absorbance ratios.

label_protocol: aRNA was retro-transcribed and directly labelled with [alpha-33P]dATP as described for polyA+RNA (Decraene, 1999). 500ng of aRNA was mixed with 500ng of random hexamers in a volume of 25µl. The mixture was incubated at 70°C for 10 min and chilled on ice. cDNA was synthesised by the addition of 5µl 10X PCR buffer, 5µl 25mM MgCl2, 5µl 0,1 mM DTT, 2,5µl 10mM mix dGTP, dCTP and dTTP, 2,5µl water, 50µCi [alpha-33P]dATP and 200U Superscript II (Invitrogen) at 42°C for 50min. The RNA template was removed by the addition of 1µl RNAse H- and incubation at 37°C for 20 min. PCR-amplified cDNA was labelled with [alpha-33P]dATP using random hexamers and Klenow included in Atlas SMART Probe Amplification kit (Clontech). 500ng of amplified cDNA was mixed with 500ng random hexamers in a volume of 34µl. The mixture was incubated at 98°C for 8 min and at 50°C for 3 min. After addition of 5µl 10X buffer, 5µl dNTPs for ATP label, 5µl [alpha-33P]dATP and 1µl Klenow, the reaction mixture was incubated at 50°C for 30 min and stopped with 2µl 0,5M EDTA. Labelled targets were then purified on Sephadex columns (G-50).

extract_protocol: IVT amplified RNA from each sample was synthesized with the MessageAmpTM aRNA Kit (Ambion) according to the manufacturer instructions (RT on 1µg total RNA, IVT during 10h). aRNA was purified on Mini Quick Spin RNA columns (Roche Diagnostic) and its quality verified on RNA 6000 lab-chips (BioAnalyser 2100; Agilent Technologies). PCR amplified cDNA was prepared as described (Revel, 1995; Pacheco-Trigon, 2002) with few modifications. Briefly, 1µg total RNA was incubated with 1µl 10µM oligo(dT), 1µl 10 mM dNTPs, 1µl 10% NP40, 1µl 20mM DTT, 2µl first-strand buffer 5X, 1µl RNAse inhibitor (Ambion) at 65°C for 2 min, at room temperature for 3 min and cooled on ice. cDNA was synthesised by the addition of 200U Superscript II (Invitrogen) and 2U AMV (Gibco BRL) and incubation at 42°C for 30 min. First-strand cDNA were poly(dG)-tailed by incubation with 1µl 20mM dGTP, 4µl TdT buffer 5x, 2,5µl water, 2,5µl TdT enzyme (Promega) at 37°C for 1h. The first PCR was performed in a volume of 50µl using 1/10 of the RT and the secondPCR was performed on 1/4 of the first PCR. Samples were incubated at 94°C for 10min before the two rounds of PCR cycles (12 cycles each; 94°C for 2 min, 63°C for 50 sec and 72°C for 6 min). PCR products were then purified using Qiaquick PCR purification (Qiagen) and their quality verified on DNA 7500 lab-chips (BioAnalyser 2100; Agilent Technologies).

treatment_protocol: Tissues were collected and immediately snap-frozen. Total RNA from ovoid (n=4), tubular (n=4) and filamentous (n=4) embryos was extracted with RNA-PlusTM (QBioGene). RNA quality was first verified by intact ribosomal bands on a 1% agarose gel (28S and 18S) and A260/280 absorbance ratios.

tag: Day of pregnancy

tag: Somatic tissue

value: no

tag: Embryo

value: yes

tag: Additional data

value: Embryo (or blastocyst) size:1 to 12 mm (ovoid stage), 50-60 mm (tubular stage), 140-160 mm (filamentous early stage). Pregnant cows from Charolais breed. Animals humanly put down in accredited experimental slaughterhouse of INRA under the supervision of veterinary services.


title: IVT_tubular_1_2

description: amplification mode=IVT  biological material=tubular embryo  target replicate=1  array replicate=2.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_filamentous_2_2

description: amplification mode=IVT  biological material=filamentous embryo  target replicate=2  array replicate=2.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.

source_name: Embryonic tissues immediately snap-frozen. Total RNA extracted with RNA-PlusTM (QBioGene). RNA quality verified by intact ribosomal bands on a 1% agarose gel (28S and 18S) and A260/280 absorbance ratios


title: PCR_tubular_1_4

description: amplification mode=PCR  biological material=tubular embryo  target replicate=1  array replicate=4.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_ovoid_1_3

description: amplification mode=IVT  biological material=ovoid embryo  target replicate=1  array replicate=3.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_filamentous_2_4

description: amplification mode=IVT  biological material=filamentous embryo  target replicate=2  array replicate=4.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_filamentous_1_3

description: amplification mode=IVT  biological material=filamentous embryo  target replicate=1  array replicate=3.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: PCR_ovoid_2_2

description: amplification mode=PCR  biological material=ovoid embryo  target replicate=2  array replicate=2.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: PCR_tubular_2_4

description: amplification mode=PCR  biological material=tubular embryo  target replicate=2  array replicate=4.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: PCR_tubular_2_1

description: amplification mode=PCR  biological material=tubular embryo  target replicate=2  array replicate=1.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: PCR_filamentous_1_1

description: amplification mode=PCR  biological material=filamentous embryo  target replicate=1    array replicate=1.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: PCR_ovary_3_3

description: amplification mode=PCR  biological material=ovoid embryo  target replicate=3  array replicate=3.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.

source_name: Ovary pieces immediately snap-frozen. Total RNA extracted with RNA-PlusTM (QBioGene). RNA quality  verified by intact ribosomal bands on a 1% agarose gel (28S and 18S) and A260/280 absorbance ratios.


title: PCR_ovary_2_2

description: amplification mode=PCR  biological material=ovary  target replicate=2  array replicate=2.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: PCR_filamentous_1_3

description: amplification mode=PCR  biological material=filamentous embryo  target replicate=1  array replicate=3.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: PCR_filamentous_2_4

description: amplification mode=PCR  biological material=filamentous embryo  target replicate=2  array replicate=4.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: PCR_filamentous_1_4

description: amplification mode=PCR  biological material=filamentous embryo  target replicate=1  array replicate=4.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: PCR_ovary_2_3

description: amplification mode=PCR  biological material=ovary  target replicate=2  array replicate=3.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: PCR_filamentous_1_2

description: amplification mode=PCR  biological material=filamentous embryo  target replicate=1  array replicate=2.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_tubular_1_1

description: amplification mode=IVT  biological material=tubular embryo  target replicate=1  array replicate=1.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: PCR_ovary_2_4

description: amplification mode=PCR  biological material=ovary  target replicate=2  array replicate=4.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_ovoid_1_1

description: amplification mode=IVT  biological material=ovoid embryo  target replicate=1  array replicate=1.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: PCR_brain_2_4

description: amplification mode=PCR  biological material=brain  target replicate=2  array replicate=4.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.

source_name: Brain pieces immediately snap-frozen. Total RNA extracted with RNA-PlusTM (QBioGene). RNA quality  verified by intact ribosomal bands on a 1% agarose gel (28S and 18S) and A260/280 absorbance ratios.

tag: animal


title: PCR_brain_3_1

description: amplification mode=PCR  biological material=brain  target replicate=3  array replicate=1.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_tubular_2_4

description: amplification mode=IVT  biological material=tubular embryo  target replicate=2  array replicate=4.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_tubular_1_4

description: amplification mode=IVT  biological material=tubular embryo  target replicate=1  array replicate=4.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: PCR_brain_2_1

description: amplification mode=PCR  biological material=brain  target replicate=2  array replicate=1.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_filamentous_2_3

description: amplification mode=IVT  biological material=filamentous embryo  target replicate=2  array replicate=3.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_filamentous_1_1

description: amplification mode=IVT  biological material=filamentous embryo  target replicate=1  array replicate=1.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_ovoid_1_2

description: amplification mode=IVT  biological material=ovoid embryo  target replicate=1  array replicate=2.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_filamentous_2_1

description: amplification mode=IVT  biological material=filamentous embryo  target replicate=2  array replicate=1.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: PCR_brain_1_4

description: amplification mode=PCR  biological material=brain  target replicate=1  array replicate=4.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_ovary_1_3

description: amplification mode=IVT  biological material=ovary  target replicate=1  array replicate=3.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: PCR_ovary_1_1

description: amplification mode=PCR  biological material=ovary  target replicate=1  array replicate=1.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_brain_3_4

description: amplification mode=IVT  biological material=brain  target replicate=3  array replicate=4.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: PCR_brain_3_2

description: amplification mode=PCR  biological material=brain  target replicate=3  array replicate=2.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_ovary_2_4

description: amplification mode=IVT  biological material=ovary  target replicate=2  array replicate=4.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: PCR_tubular_2_2

description: amplification mode=PCR  biological material=tubular embryo  target replicate=2  array replicate=2.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_ovary_1_1

description: amplification mode=IVT  biological material=ovary  target replicate=1  array replicate=1.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_filamentous_1_4

description: amplification mode=IVT  biological material=filamentous embryo  target replicate=1  array replicate=4.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_ovoid_2_4

description: amplification mode=IVT  biological material=ovoid embryo  target replicate=2  array replicate=4.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: PCR_ovary_3_2

description: amplification mode=PCR  biological material=ovary  target replicate=3  array replicate=2.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: PCR_filamentous_2_2

description: amplification mode=PCR  biological material=filamentous embryo  target replicate=2  array replicate=2.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: PCR_ovary_1_2

description: amplification mode=PCR  biological material=ovary  target replicate=1  array replicate=2.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_brain_3_2

description: amplification mode=IVT  biological material=brain  target replicate=3  array replicate=2.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: PCR_brain_3_3

description: amplification mode=PCR  biological material=brain  target replicate=3  array replicate=3.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_tubular_1_3

description: amplification mode=IVT  biological material=tubular embryo  target replicate=1  array replicate=3.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: PCR_brain_3_4

description: amplification mode=PCR  biological material=brain  target replicate=3  array replicate=4.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_ovary_1_2

description: amplification mode=IVT  biological material=ovary  target replicate=1  array replicate=2.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_ovary_3_4

description: amplification mode=IVT  biological material=ovary  target replicate=3  array replicate=4.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_brain_2_2

description: amplification mode=IVT  biological material=brain  target replicate=2  array replicate=2.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_ovary_2_2

description: amplification mode=IVT  biological material=ovary  target replicate=2  array replicate=2.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_brain_3_1

description: amplification mode=IVT  biological material=brain  target replicate=3  array replicate=1.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_ovary_3_3

description: amplification mode=IVT  biological material=ovary  target replicate=3  array replicate=3.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: PCR_filamentous_2_3

description: amplification mode=PCR  biological material=filamentous embryo  target replicate=2  array replicate=3.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_brain_2_1

description: amplification mode=IVT  biological material=brain  target replicate=2  array replicate=1.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_ovoid_2_3

description: amplification mode=IVT  biological material=ovoid embryo  target replicate=2  array replicate=3.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_tubular_2_2

description: amplification mode=IVT  biological material=tubular embryo  target replicate=2  array replicate=2.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_tubular_2_3

description: amplification mode=IVT  biological material=tubular embryo  target replicate=2  array replicate=3.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: PCR_brain_2_3

description: amplification mode=PCR  biological material=brain  target replicate=2  array replicate=3.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_ovoid_1_4

description: amplification mode=IVT  biological material=ovoid embryo  target replicate=1  array replicate=4.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_filamentous_1_2

description: amplification mode=IVT  biological material=filamentous embryo  target replicate=1  array replicate=2.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: PCR_brain_2_2

description: amplification mode=PCR  biological material=brain  target replicate=2  array replicate=2.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_brain_1_3

description: amplification mode=IVT  biological material=brain  target replicate=1  array replicate=3.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_ovary_2_1

description: amplification mode=IVT  biological material=ovary  target replicate=2  array replicate=1.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: PCR_brain_1_3

description: amplification mode=PCR  biological material=brain  target replicate=1  array replicate=3.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: PCR_brain_1_1

description: amplification mode=PCR  biological material=brain  target replicate=1  array replicate=1.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_ovary_2_3

description: amplification mode=IVT  biological material=ovary  target replicate=2  array replicate=3.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_brain_3_3

description: amplification mode=IVT  biological material=brain  target replicate=3  array replicate=3.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_brain_1_1

description: amplification mode=IVT  biological material=brain  target replicate=1  array replicate=1.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: PCR_ovary_1_4

description: amplification mode=PCR  biological material=ovary  target replicate=1  array replicate=4.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_ovoid_2_1

description: amplification mode=IVT  biological material=ovoid embryo  target replicate=2  array replicate=1.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: PCR_ovoid_1_1

description: amplification mode=PCR  biological material=ovoid embryo  target replicate=1  array replicate=1.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_brain_2_3

description: amplification mode=IVT  biological material=brain  target replicate=2  array replicate=3.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_brain_1_2

description: amplification mode=IVT  biological material=brain  target replicate=1  array replicate=2.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: PCR_ovary_2_1

description: amplification mode=PCR  biological material=ovary  target replicate=2  array replicate=1.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_brain_2_4

description: amplification mode=IVT  biological material=brain  target replicate=2  array replicate=4.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_ovary_3_2

description: amplification mode=IVT  biological material=ovary  target replicate=3  array replicate=2.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: PCR_brain_1_2

description: amplification mode=PCR  biological material=brain  target replicate=1  array replicate=2.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_ovary_1_4

description: amplification mode=IVT  biological material=ovary  target replicate=1  array replicate=4.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: PCR_ovary_3_4

description: amplification mode=PCR  biological material=ovary  target replicate=3  array replicate=4.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


title: IVT_ovary_3_1

description: amplification mode=IVT  biological material=ovary  target replicate=3  array replicate=1.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


status: Public on Dec 18 2007

city: Houston

first: Chad

middle: A

last: Shaw

state: TX

address: One Baylor Plaza

department: Department of Neuroscience

country: USA

institute: Baylor College of Medicine

description:
Sca1cerebellum-wild type-biological rep2
gene expression data

hyb_protocol: Following fragmentation of 15.0 mg of the labeled cRNA, a hybridization cocktail containing Affymetrix spike-in controls and fragmented labeled cRNA was loaded onto a GeneChip array.  The array was hybridized 16 hours at 45 degrees C with rotation at 60 rpm, then washed with a strepavidin, R-phycoerythrin conjugate stain.  Signal amplification was done using biotinylated antistreptavidin.

scan_protocol: The array was stained using the EukGEws2v5 protocol on the Fluidics Station 400.  The stained array was scanned on the Affymetrix GeneChip Scanner 3000.

data_processing: The images were analyzed and quality control metrics recorded using Affymetrix GCOS software version 1.1.

label: biotin

organism: Mus musculus

source_name: cerebellum, Sca1 wild type

label_protocol: Total RNA was reverse transcribed to produce double-stranded cDNA.  The cDNA product was used as a template for the in vitro transcription reaction, producing biotin-labeled cRNA.  The labeled cRNA was quantified using the NanoDrop ND-1000 machine.  15.0 mg of the labeled cRNA was fragmented and re-checked on NanoDrop for concentration.

extract_protocol: Trizol extraction of total cerebellar RNA was performed, followed by Qiagen RNeasy clean-up, both according to manufacturers' instructions.

tag: genotype

value: wild type

tag: gender

value: female

tag: age

value: 4 weeks

tag: strain

value: C57BL/6J, 5-8 generations backcrossed


title: IVT_brain_1_4

description: amplification mode=IVT  biological material=brain  target replicate=1  array replicate=4.  As advised by N'Guyen (1995), we first determined the amount of labelled target to be used for each hybridisation so that no additional signal appeared but the intensity of the positive signals increased when the amount of target did. (50, 125, 250 and 500ng for aRNA or cDNA labelled targets). On this basis, 125ng of each target has been hybridised to each array.


description:
Sca1cerebellum-wild type-biological rep3
gene expression data


description:
Sca1cerebellum-wild type-biological rep4
gene expression data

value: 9 weeks


description:
Sca1cerebellum-wild type-biological rep6
gene expression data

value: 12 weeks


title: Sca1cerebellum-knock-in-biological rep6

description:
Sca1cerebellum-knock-in-biological rep6
gene expression data

source_name: cerebellum, Sca1 knock-in

value: knock-in


description:
Sca1cerebellum-wild type-biological rep1
gene expression data


title: Sca1cerebellum-knock-in-biological rep5

description:
Sca1cerebellum-knock-in-biological rep5
gene expression data


title: Sca1cerebellum-knock-in biological rep3

description:
Sca1cerebellum-knock-in biological rep3
gene expression data


description:
Sca1cerebellum-wild type-biological rep5
gene expression data


title: Sca1cerebellum-knock-in-biological rep4

description:
Sca1cerebellum-knock-in-biological rep4
gene expression data


title: Sca1cerebellum-knock-in-biological rep1

description:
Sca1cerebellum-knock-in-biological rep1
gene expression data

source_name: cerebellum, Sca1 knock- in


title: Sca1cerebellum-knock-in-biological rep2

description:
Sca1cerebellum-knock-in-biological rep2
gene expression data


title: KD3133 5d D R

status: Public on Dec 13 2007

city: Stanford

first: Stephen

last: Popper

state: CA

department: Division of Infectious Diseases, Dept. of Medicine

institute: Stanford University

description:
Phenotype: Patient responded (R) or not (NR) to IVIG therapy. Some patients were not classified because they were treated more than 10 days after onset of fever
Image: http://smd.stanford.edu/MicroArray/gifs/2004-07/53976.gif
Measurement: time(relative) 5 d
Individual: KD3133
Phenotype: R

hyb_protocol: not provided

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1100, 5190; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Stephen,,Popper Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.55; Parameter Scan Time = 12:15:39; Parameter PMT Volts = 580; Parameter Scan Date = 2004-07-15; Parameter Pixel Size = 10; Parameter Laser on time = 182138; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.92; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.55; Parameter Scan Time = 12:15:39; Parameter PMT Volts = 610; Parameter Scan Date = 2004-07-15; Parameter Pixel Size = 10; Parameter Laser on time = 401106; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.71; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper

data_processing: VALUE is Log (base 2) of the ratio of the median of Channel 2 (usually 635 nm) to Channel 1 (usually 532 nm)

organism: Homo sapiens

source_name: Stratagene Universal Human Ref, amplified along with samples 061604 and pooled

label_protocol: not provided

extract_protocol: not provided

tag: Simple annotation

value: Infection, Whole blood

tag: Measurement

value: time(relative) 5 d

tag: Individual

tag: Phenotype

value: R


title: KD3134 6d D R

description:
Phenotype: Patient responded (R) or not (NR) to IVIG therapy. Some patients were not classified because they were treated more than 10 days after onset of fever
Image: http://smd.stanford.edu/MicroArray/gifs/2004-07/53977.gif
Measurement: time(relative) 6 d
Individual: KD3134
Phenotype: R

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1100, 5190; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Stephen,,Popper Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.58; Parameter Scan Time = 12:02:41; Parameter PMT Volts = 560; Parameter Scan Date = 2004-07-15; Parameter Pixel Size = 10; Parameter Laser on time = 182126; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.91; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.58; Parameter Scan Time = 12:02:41; Parameter PMT Volts = 620; Parameter Scan Date = 2004-07-15; Parameter Pixel Size = 10; Parameter Laser on time = 401094; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.72; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper

value: time(relative) 6 d


title: KD3040 8d N R

description:
Phenotype: Patient responded (R) or not (NR) to IVIG therapy. Some patients were not classified because they were treated more than 10 days after onset of fever
Image: http://smd.stanford.edu/MicroArray/gifs/2004-07/53760.gif
Measurement: time(relative) 8 d
Individual: KD3040
Phenotype: R

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1200, 5270; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Stephen,,Popper Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.79; Parameter Scan Time = 15:50:01; Parameter PMT Volts = 570; Parameter Scan Date = 2004-07-11; Parameter Pixel Size = 10; Parameter Laser on time = 179925; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 3.28; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.79; Parameter Scan Time = 15:50:01; Parameter PMT Volts = 700; Parameter Scan Date = 2004-07-11; Parameter Pixel Size = 10; Parameter Laser on time = 398893; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.72; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper

value: time(relative) 8 d


title: KD3108 5d D R

description:
Phenotype: Patient responded (R) or not (NR) to IVIG therapy. Some patients were not classified because they were treated more than 10 days after onset of fever
Image: http://smd.stanford.edu/MicroArray/gifs/2004-07/53978.gif
Measurement: time(relative) 5 d
Individual: KD3108
Phenotype: R

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1100, 5190; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Stephen,,Popper Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.45; Parameter Scan Time = 11:08:30; Parameter PMT Volts = 560; Parameter Scan Date = 2004-07-15; Parameter Pixel Size = 10; Parameter Laser on time = 182070; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.17; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.45; Parameter Scan Time = 11:08:30; Parameter PMT Volts = 670; Parameter Scan Date = 2004-07-15; Parameter Pixel Size = 10; Parameter Laser on time = 401038; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.73; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper


title: KD3013 11d A

description:
Phenotype: Patient responded (R) or not (NR) to IVIG therapy. Some patients were not classified because they were treated more than 10 days after onset of fever
Image: http://smd.stanford.edu/MicroArray/gifs/2004-07/53542.gif
Measurement: time(relative) 11 d
Individual: KD3013
Phenotype: A

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1260, 5200; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Stephen,,Popper Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 32.89; Parameter Scan Time = 16:17:38; Parameter PMT Volts = 540; Parameter Scan Date = 2004-07-07; Parameter Pixel Size = 10; Parameter Laser on time = 178014; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 3.74; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 32.89; Parameter Scan Time = 16:17:38; Parameter PMT Volts = 700; Parameter Scan Date = 2004-07-07; Parameter Pixel Size = 10; Parameter Laser on time = 396982; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.70; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper

value: time(relative) 11 d

value: A


title: KD3015 11d N

description:
Phenotype: Patient responded (R) or not (NR) to IVIG therapy. Some patients were not classified because they were treated more than 10 days after onset of fever
Image: http://smd.stanford.edu/MicroArray/gifs/2004-07/53544.gif
Measurement: time(relative) 11 d
Individual: KD3015
Phenotype: N

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 940, 5550; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Stephen,,Popper Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 32.86; Parameter Scan Time = 16:48:07; Parameter PMT Volts = 540; Parameter Scan Date = 2004-07-07; Parameter Pixel Size = 10; Parameter Laser on time = 178044; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 3.79; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 32.86; Parameter Scan Time = 16:48:07; Parameter PMT Volts = 690; Parameter Scan Date = 2004-07-07; Parameter Pixel Size = 10; Parameter Laser on time = 397012; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.70; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper

value: N


title: KD3014 6d N R

description:
Phenotype: Patient responded (R) or not (NR) to IVIG therapy. Some patients were not classified because they were treated more than 10 days after onset of fever
Image: http://smd.stanford.edu/MicroArray/gifs/2004-07/53543.gif
Measurement: time(relative) 6 d
Individual: KD3014
Phenotype: R

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1260, 5340; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Stephen,,Popper Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 32.86; Parameter Scan Time = 16:30:24; Parameter PMT Volts = 540; Parameter Scan Date = 2004-07-07; Parameter Pixel Size = 10; Parameter Laser on time = 178026; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 3.77; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 32.86; Parameter Scan Time = 16:30:24; Parameter PMT Volts = 690; Parameter Scan Date = 2004-07-07; Parameter Pixel Size = 10; Parameter Laser on time = 396995; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.69; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper


title: KD3018 6d D R

description:
Phenotype: Patient responded (R) or not (NR) to IVIG therapy. Some patients were not classified because they were treated more than 10 days after onset of fever
Image: http://smd.stanford.edu/MicroArray/gifs/2004-07/53546.gif
Measurement: time(relative) 6 d
Individual: KD3018
Phenotype: R

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 860, 5630; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Stephen,,Popper Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 32.89; Parameter Scan Time = 17:25:03; Parameter PMT Volts = 570; Parameter Scan Date = 2004-07-07; Parameter Pixel Size = 10; Parameter Laser on time = 178081; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 3.73; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 32.89; Parameter Scan Time = 17:25:03; Parameter PMT Volts = 690; Parameter Scan Date = 2004-07-07; Parameter Pixel Size = 10; Parameter Laser on time = 397049; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.70; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper


title: KD3016 7d D NR

description:
Phenotype: Patient responded (R) or not (NR) to IVIG therapy. Some patients were not classified because they were treated more than 10 days after onset of fever
Image: http://smd.stanford.edu/MicroArray/gifs/2004-07/53545.gif
Measurement: time(relative) 7 d
Individual: KD3016
Phenotype: NR

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 940, 5350; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Stephen,,Popper Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 32.89; Parameter Scan Time = 17:02:11; Parameter PMT Volts = 550; Parameter Scan Date = 2004-07-07; Parameter Pixel Size = 10; Parameter Laser on time = 178059; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 3.74; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 32.89; Parameter Scan Time = 17:02:11; Parameter PMT Volts = 690; Parameter Scan Date = 2004-07-07; Parameter Pixel Size = 10; Parameter Laser on time = 397027; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.70; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper

value: time(relative) 7 d

value: NR


title: KD3019 8d D R

description:
Phenotype: Patient responded (R) or not (NR) to IVIG therapy. Some patients were not classified because they were treated more than 10 days after onset of fever
Image: http://smd.stanford.edu/MicroArray/gifs/2004-07/53547.gif
Measurement: time(relative) 8 d
Individual: KD3019
Phenotype: R

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 860, 5240; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Stephen,,Popper Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 32.89; Parameter Scan Time = 17:38:46; Parameter PMT Volts = 570; Parameter Scan Date = 2004-07-07; Parameter Pixel Size = 10; Parameter Laser on time = 178096; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 3.69; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 32.89; Parameter Scan Time = 17:38:46; Parameter PMT Volts = 720; Parameter Scan Date = 2004-07-07; Parameter Pixel Size = 10; Parameter Laser on time = 397064; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.69; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper


title: KD3126 5d D R

description:
Phenotype: Patient responded (R) or not (NR) to IVIG therapy. Some patients were not classified because they were treated more than 10 days after onset of fever
Image: http://smd.stanford.edu/MicroArray/gifs/2004-07/53973.gif
Measurement: time(relative) 5 d
Individual: KD3126
Phenotype: R

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1360, 5490; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Stephen,,Popper Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.76; Parameter Scan Time = 13:31:29; Parameter PMT Volts = 550; Parameter Scan Date = 2004-07-15; Parameter Pixel Size = 10; Parameter Laser on time = 182214; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 3.13; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.76; Parameter Scan Time = 13:31:29; Parameter PMT Volts = 640; Parameter Scan Date = 2004-07-15; Parameter Pixel Size = 10; Parameter Laser on time = 401182; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.72; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper


title: KD3121 6d N R

description:
Phenotype: Patient responded (R) or not (NR) to IVIG therapy. Some patients were not classified because they were treated more than 10 days after onset of fever
Image: http://smd.stanford.edu/MicroArray/gifs/2004-07/53970.gif
Measurement: time(relative) 6 d
Individual: KD3121
Phenotype: R

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1160, 5830; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Stephen,,Popper Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.82; Parameter Scan Time = 14:47:20; Parameter PMT Volts = 550; Parameter Scan Date = 2004-07-15; Parameter Pixel Size = 10; Parameter Laser on time = 182290; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.96; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.82; Parameter Scan Time = 14:47:20; Parameter PMT Volts = 630; Parameter Scan Date = 2004-07-15; Parameter Pixel Size = 10; Parameter Laser on time = 401258; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.72; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper


title: KD3065 11d N

description:
Phenotype: Patient responded (R) or not (NR) to IVIG therapy. Some patients were not classified because they were treated more than 10 days after onset of fever
Image: http://smd.stanford.edu/MicroArray/gifs/2004-07/53791.gif
Measurement: time(relative) 11 d
Individual: KD3065
Phenotype: N

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 980, 5130; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Stephen,,Popper Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.97; Parameter Scan Time = 18:55:22; Parameter PMT Volts = 560; Parameter Scan Date = 2004-07-11; Parameter Pixel Size = 10; Parameter Laser on time = 180109; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.75; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.97; Parameter Scan Time = 18:55:22; Parameter PMT Volts = 710; Parameter Scan Date = 2004-07-11; Parameter Pixel Size = 10; Parameter Laser on time = 399077; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.71; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper


title: KD3012 11d D

description:
Phenotype: Patient responded (R) or not (NR) to IVIG therapy. Some patients were not classified because they were treated more than 10 days after onset of fever
Image: http://smd.stanford.edu/MicroArray/gifs/2004-07/53541.gif
Measurement: time(relative) 11 d
Individual: KD3012
Phenotype: D

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 880, 5490; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Stephen,,Popper Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 32.86; Parameter Scan Time = 16:04:29; Parameter PMT Volts = 540; Parameter Scan Date = 2004-07-07; Parameter Pixel Size = 10; Parameter Laser on time = 178002; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 3.30; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 32.86; Parameter Scan Time = 16:04:29; Parameter PMT Volts = 700; Parameter Scan Date = 2004-07-07; Parameter Pixel Size = 10; Parameter Laser on time = 396970; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.69; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper

value: D


title: KD3130 6d N R

description:
Phenotype: Patient responded (R) or not (NR) to IVIG therapy. Some patients were not classified because they were treated more than 10 days after onset of fever
Image: http://smd.stanford.edu/MicroArray/gifs/2004-07/53975.gif
Measurement: time(relative) 6 d
Individual: KD3130
Phenotype: R

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1400, 4910; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Stephen,,Popper Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.70; Parameter Scan Time = 12:58:03; Parameter PMT Volts = 560; Parameter Scan Date = 2004-07-15; Parameter Pixel Size = 10; Parameter Laser on time = 182180; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 3.15; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.70; Parameter Scan Time = 12:58:03; Parameter PMT Volts = 640; Parameter Scan Date = 2004-07-15; Parameter Pixel Size = 10; Parameter Laser on time = 401149; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.72; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper


title: KD3125 5d D

description:
Phenotype: Patient responded (R) or not (NR) to IVIG therapy. Some patients were not classified because they were treated more than 10 days after onset of fever
Image: http://smd.stanford.edu/MicroArray/gifs/2004-07/53972.gif
Measurement: time(relative) 5 d
Individual: KD3125
Phenotype: D

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1360, 5090; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Stephen,,Popper Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.82; Parameter Scan Time = 13:43:58; Parameter PMT Volts = 550; Parameter Scan Date = 2004-07-15; Parameter Pixel Size = 10; Parameter Laser on time = 182226; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.68; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.82; Parameter Scan Time = 13:43:58; Parameter PMT Volts = 660; Parameter Scan Date = 2004-07-15; Parameter Pixel Size = 10; Parameter Laser on time = 401194; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.72; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper


title: KD1523 7d N R

description:
Phenotype: Patient responded (R) or not (NR) to IVIG therapy. Some patients were not classified because they were treated more than 10 days after onset of fever
Image: http://smd.stanford.edu/MicroArray/gifs/2004-07/53711.gif
Measurement: time(relative) 7 d
Individual: KD1523
Phenotype: R

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1360, 5880; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Stephen,,Popper Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 30.87; Parameter Scan Time = 10:22:58; Parameter PMT Volts = 550; Parameter Scan Date = 2004-07-11; Parameter Pixel Size = 10; Parameter Laser on time = 179597; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 3.47; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 30.87; Parameter Scan Time = 10:22:58; Parameter PMT Volts = 740; Parameter Scan Date = 2004-07-11; Parameter Pixel Size = 10; Parameter Laser on time = 398566; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.73; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper


title: KD3034 9d N R

description:
Phenotype: Patient responded (R) or not (NR) to IVIG therapy. Some patients were not classified because they were treated more than 10 days after onset of fever
Image: http://smd.stanford.edu/MicroArray/gifs/2004-07/53750.gif
Measurement: time(relative) 9 d
Individual: KD3034
Phenotype: R

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1280, 5240; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Stephen,,Popper Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.67; Parameter Scan Time = 13:21:15; Parameter PMT Volts = 580; Parameter Scan Date = 2004-07-11; Parameter Pixel Size = 10; Parameter Laser on time = 179775; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 3.04; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.67; Parameter Scan Time = 13:21:15; Parameter PMT Volts = 700; Parameter Scan Date = 2004-07-11; Parameter Pixel Size = 10; Parameter Laser on time = 398743; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.71; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper

value: time(relative) 9 d


title: KD1538 8d D R

description:
Phenotype: Patient responded (R) or not (NR) to IVIG therapy. Some patients were not classified because they were treated more than 10 days after onset of fever
Image: http://smd.stanford.edu/MicroArray/gifs/2004-07/53716.gif
Measurement: time(relative) 8 d
Individual: KD1538
Phenotype: R

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1280, 5690; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Stephen,,Popper Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.18; Parameter Scan Time = 10:38:56; Parameter PMT Volts = 580; Parameter Scan Date = 2004-07-11; Parameter Pixel Size = 10; Parameter Laser on time = 179613; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.87; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.18; Parameter Scan Time = 10:38:56; Parameter PMT Volts = 710; Parameter Scan Date = 2004-07-11; Parameter Pixel Size = 10; Parameter Laser on time = 398581; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.71; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper


title: KD3127 4d N R

description:
Phenotype: Patient responded (R) or not (NR) to IVIG therapy. Some patients were not classified because they were treated more than 10 days after onset of fever
Image: http://smd.stanford.edu/MicroArray/gifs/2004-07/53974.gif
Measurement: time(relative) 4 d
Individual: KD3127
Phenotype: R

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1440, 5110; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Stephen,,Popper Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.73; Parameter Scan Time = 13:18:42; Parameter PMT Volts = 570; Parameter Scan Date = 2004-07-15; Parameter Pixel Size = 10; Parameter Laser on time = 182202; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 3.01; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.73; Parameter Scan Time = 13:18:42; Parameter PMT Volts = 620; Parameter Scan Date = 2004-07-15; Parameter Pixel Size = 10; Parameter Laser on time = 401170; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.72; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper

value: time(relative) 4 d


title: KD3066 5d N R

description:
Phenotype: Patient responded (R) or not (NR) to IVIG therapy. Some patients were not classified because they were treated more than 10 days after onset of fever
Image: http://smd.stanford.edu/MicroArray/gifs/2004-07/53794.gif
Measurement: time(relative) 5 d
Individual: KD3066
Phenotype: R

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 980, 5130; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Stephen,,Popper Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 32.03; Parameter Scan Time = 19:08:18; Parameter PMT Volts = 560; Parameter Scan Date = 2004-07-11; Parameter Pixel Size = 10; Parameter Laser on time = 180122; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.76; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 32.03; Parameter Scan Time = 19:08:18; Parameter PMT Volts = 700; Parameter Scan Date = 2004-07-11; Parameter Pixel Size = 10; Parameter Laser on time = 399091; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.71; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper


title: KD3036 5d N NR

description:
Phenotype: Patient responded (R) or not (NR) to IVIG therapy. Some patients were not classified because they were treated more than 10 days after onset of fever
Image: http://smd.stanford.edu/MicroArray/gifs/2004-07/53757.gif
Measurement: time(relative) 5 d
Individual: KD3036
Phenotype: NR

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1120, 5450; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Stephen,,Popper Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.48; Parameter Scan Time = 15:13:05; Parameter PMT Volts = 590; Parameter Scan Date = 2004-07-11; Parameter Pixel Size = 10; Parameter Laser on time = 179888; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 3.01; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.48; Parameter Scan Time = 15:13:05; Parameter PMT Volts = 700; Parameter Scan Date = 2004-07-11; Parameter Pixel Size = 10; Parameter Laser on time = 398856; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.71; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper


title: KD3067 4d N R

description:
Phenotype: Patient responded (R) or not (NR) to IVIG therapy. Some patients were not classified because they were treated more than 10 days after onset of fever
Image: http://smd.stanford.edu/MicroArray/gifs/2004-07/53798.gif
Measurement: time(relative) 4 d
Individual: KD3067
Phenotype: R

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 980, 5130; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Stephen,,Popper Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 32.00; Parameter Scan Time = 20:28:42; Parameter PMT Volts = 560; Parameter Scan Date = 2004-07-11; Parameter Pixel Size = 10; Parameter Laser on time = 180203; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.63; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 32.00; Parameter Scan Time = 20:28:42; Parameter PMT Volts = 770; Parameter Scan Date = 2004-07-11; Parameter Pixel Size = 10; Parameter Laser on time = 399171; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.71; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper


title: KD1500 4d N R

description:
Phenotype: Patient responded (R) or not (NR) to IVIG therapy. Some patients were not classified because they were treated more than 10 days after onset of fever
Image: http://smd.stanford.edu/MicroArray/gifs/2004-07/53533.gif
Measurement: time(relative) 4 d
Individual: KD1500
Phenotype: R

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1200, 5240; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Stephen,,Popper Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 32.58; Parameter Scan Time = 13:34:05; Parameter PMT Volts = 560; Parameter Scan Date = 2004-07-07; Parameter Pixel Size = 10; Parameter Laser on time = 177852; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 3.12; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 32.58; Parameter Scan Time = 13:34:05; Parameter PMT Volts = 710; Parameter Scan Date = 2004-07-07; Parameter Pixel Size = 10; Parameter Laser on time = 396820; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.68; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper


title: KD3123 2d N R

description:
Phenotype: Patient responded (R) or not (NR) to IVIG therapy. Some patients were not classified because they were treated more than 10 days after onset of fever
Image: http://smd.stanford.edu/MicroArray/gifs/2004-07/53971.gif
Measurement: time(relative) 2 d
Individual: KD3123
Phenotype: R

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1440, 5090; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Stephen,,Popper Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.85; Parameter Scan Time = 14:34:34; Parameter PMT Volts = 550; Parameter Scan Date = 2004-07-15; Parameter Pixel Size = 10; Parameter Laser on time = 182276; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.94; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.85; Parameter Scan Time = 14:34:34; Parameter PMT Volts = 650; Parameter Scan Date = 2004-07-15; Parameter Pixel Size = 10; Parameter Laser on time = 401245; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.72; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper

value: time(relative) 2 d


title: KD3120 5d N R

description:
Phenotype: Patient responded (R) or not (NR) to IVIG therapy. Some patients were not classified because they were treated more than 10 days after onset of fever
Image: http://smd.stanford.edu/MicroArray/gifs/2004-07/53969.gif
Measurement: time(relative) 5 d
Individual: KD3120
Phenotype: R

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Image Origin = 1240, 5710; Parameter Datafile type = GenePix Results 3; Software: type: feature extraction; Performer: Stephen,,Popper Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.94; Parameter Scan Time = 15:34:34; Parameter PMT Volts = 540; Parameter Scan Date = 2004-07-15; Parameter Pixel Size = 10; Parameter Laser on time = 182337; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.84; Parameter Scan Power = 100; Parameter Scanning software version = 2.0.2.2; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scan Temperature = 31.94; Parameter Scan Time = 15:34:34; Parameter PMT Volts = 610; Parameter Scan Date = 2004-07-15; Parameter Pixel Size = 10; Parameter Laser on time = 401305; Parameter Focus Position = 0; Parameter Scanner Model = GenePix 4000B [81971]; Parameter Laser Power = 2.72; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter Scanning software = AxImageIO; Performer: Stephen,,Popper