title: MyoD, mb1, 6hr, chip MG_U74Av2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S334
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_343
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time course: 6 hr
Estimated purity:				100%
Num cells for RNA prep:			More than 2 x 10E+6   Balb/c


title: MyoD, mb1, 12hr, chip MG_U74Av2

status: Public on Mar 01 2008

type: RNA

city: Ottawa

first: Theodore

last: Perkins

state: ON

address: 501 Smyth Rd.

department: Cellular and Molecular Medicine

country: Canada

institute: Ottawa Hospital Research Institute

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S335
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_344
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time course: 12 hr
Estimated purity:				100%
Num cells for RNA prep:			More than 2 x 10E+6 Balb/c

hyb_protocol: Following fragmentation, cRNA was hybridized on the GeneChip Array and the GeneChips were washed and stained in the Affymetrix Fluidics Station 450 according to the standard Affymetrix protocol  (Expression Analysis Technical Manual Rev. 2, 2005-2006, Affymetrix).

scan_protocol: GeneChips were scanned using the Affymetrix GeneChip 3000 7G scanner.

data_processing: Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpha2=0.06

label: Biotin

molecule: total RNA

organism: Mus musculus

source_name: Primary Myoblasts, Skeletal Muscle

label_protocol: Biotinylated cRNA was prepared from the total RNA according to the Affymetrix protocol (Expression Analysis Technical Manual Rev. 2, 2005-2006, Affymetrix)

growth_protocol:
Growth medium:				Ham's F10 Nutrient Mixture
Media supplements:			20% FBS,, bFGF 250 ng/ml, 4% Penicillin (5x10E3 U/ml)-Streptomycin (5x10E3 ug/ml), 1% L-Glutamine 200 mM
Culture conditions:			37ºC, 5% CO2, 0.01% Collagen coated petri plate; Change with fresh media at day 3rd.

extract_protocol:
RNA prep method:				RNeasy (Qiagen)
Cell purification method:			cultured
Contaminating cell type:			none

tag: Strain:					Balb/c MyoD wild Type

tag: Gender:					Male


title: MyoD, wtp16, 12hr, chip MG_U74Av2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S335
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_344
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time course: 12 hr
Estimated purity:				100%
RNA concentration:			1760.6 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6 Balb/c
Sample volume:				27µL


title: MyoD-/-, P10, 4d, chip MG_U74Cv2

type: other

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S351
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_360
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 4 day
Estimated purity:				100%
RNA concentration:			1130.3 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL

molecule: other

growth_protocol:
Growth medium:				Ham's F10 Nutrient Mixture
Media supplements:			20% FBS, bFGF 250 ng/ml, 4% Penicillin (5x10E3 U/ml)-Streptomycin (5x10E3 ug/ml), 1% L-Glutamine 200 mM
Culture conditions:			37ºC, 5% CO2, 0.01% Collagen coated petri plate; Change with fresh media at day 3rd.

extract_protocol: Cell purification method:			cultured

tag: Strain:					Balb/c MyoD-/-

tag: Karyotype:				normal


title: MyoD-/-,P10, 7d, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S352
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_361
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 7 day
Estimated purity:				100%
RNA concentration:			1545.2 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL


title: MyoD-/-,P6, 7d, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S352
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_361
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 7 day
Estimated purity:				100%
RNA concentration:			2347.2 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL


title: MyoD-/-,P6, 3d, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S350
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_359
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 3 day
Estimated purity:				100%
RNA concentration:			1652.8 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL


title: MyoD-/-,1999, 3d, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S350
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_359
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 3 day
Estimated purity:				100%
RNA concentration:			1937.6 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL


title: MyoD, mb1, 0hr, chip MG_U74Av2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S333
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_342
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time course: 0 hr
Estimated purity:				100%
Num cells for RNA prep:			More than 2 x 10E+6   Balb/c


title: MyoD-/-, P10, 48hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S349
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_358
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 48 hr
Estimated purity:				100%
RNA concentration:			1294.3 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL


title: MyoD-/-, P10, 3d, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S350
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_359
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 3 day
Estimated purity:				100%
RNA concentration:			1336.7 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL


title: MyoD-/-, P6, 4d, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S351
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_360
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 4 day
Estimated purity:				100%
RNA concentration:			2485.4 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL


title: MyoD, wt841, 6hr, chip MG_U74Av2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S334
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_343
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time course: 6 hr
Estimated purity:				100%
RNA concentration:			1212.4 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6   Balb/c
Sample volume:				27µL


title: MyoD-/-,1999, 7d, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S352
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_361
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 7 day
Estimated purity:				100%
RNA concentration:			1374.0 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL


title: MyoD-/-, 1999, 4d, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S351
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_360
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 4 day
Estimated purity:				100%
RNA concentration:			1815.8 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL


title: MyoD, wtp16, 6hr, chip MG_U74Av2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S334
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_343
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time course: 6 hr
Estimated purity:				100%
RNA concentration:			1393.0 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6   Balb/c
Sample volume:				27µL


title: MyoD, wt841, 0hr, chip MG_U74Av2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S333
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_342
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time course: 0 hr
Estimated purity:				100%
RNA concentration:			2297.8 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6   Balb/c
Sample volume:				27µL


title: MyoD-/-, 1999, 48hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S349
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_358
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 48 hr
Estimated purity:				100%
RNA concentration:			1110.6 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL


title: MyoD-/-, P10, 36hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S348
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_357
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 36 hr
Estimated purity:				100%
RNA concentration:			1586.8 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL


title: MyoD, wtp16, 0hr, chip MG_U74Av2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S333
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_342
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time course: 0 hr
Estimated purity:				100%
RNA concentration:			1170.2 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6   Balb/c
Sample volume:				27µL


title: MyoD-/-, 1999, 36hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S348
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_357
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 36 hr
Estimated purity:				100%
RNA concentration:			1709.4 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL


title: MyoD-/-, P10, 24hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S347
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_356
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 24 hr
Estimated purity:				100%
RNA concentration:			2229.8 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL


title: MyoD-/-, P6, 48hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S349
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_358
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 48 hr
Estimated purity:				100%
RNA concentration:			1086.4 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL


title: MyoD-/-, P6, 24hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S347
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_356
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 24 hr
Estimated purity:				100%
RNA concentration:			1879.2 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL


title: MyoD-/-, P10, 18hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S346
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_355
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 18 hr
Estimated purity:				100%
RNA concentration:			2226.4 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL


title: MyoD-/-, 1999, 18hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S346
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_355
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 18 hr
Estimated purity:				100%
RNA concentration:			1419.4 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL


title: MyoD-/-, P6, 36hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S348
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_357
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 36 hr
Estimated purity:				100%
RNA concentration:			1298.0 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL


title: MyoD-/-, P6, 18hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S346
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_355
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 18 hr
Estimated purity:				100%
RNA concentration:			1960.2 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL


title: MyoD-/-, P10, 12hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S345
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_354
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 12 hr
Estimated purity:				100%
RNA concentration:			2606.6 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL


title: MyoD-/-, P6, 12hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S345
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_354
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 12 hr
Estimated purity:				100%
RNA concentration:			2205.8 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL


title: MyoD-/-, P6, 6hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S344
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_353
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 6 hr
Estimated purity:				100%
RNA concentration:			2205.8 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL


title: MyoD-/-, 1999, 12hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S345
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_354
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 12 hr
Estimated purity:				100%
RNA concentration:			2830.4 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL


title: MyoD-/-, 1999, 6hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S344
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_353
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 6 hr
Estimated purity:				100%
RNA concentration:			2830.4 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL


title: MyoD-/-, 1999, 24hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S347
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_356
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 24 hr
Estimated purity:				100%
RNA concentration:			2583.8 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL


title: MyoD-/-, P10, 6hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S344
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_353
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 6 hr
Estimated purity:				100%
RNA concentration:			2606.6 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL


title: Nothing_4_hours, biological rep2

city: Cambridge

first: Alena

middle: A

last: Antipova

state: MA

address: 7 Cambridge Center

country: USA

institute: Broad Institute of Massachusetts Institute of Technology and Harvard University

description: Gene expression data from SH-SY5Y cells treated with DMSO for 1h, then with water for 4h.

hyb_protocol: Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.

scan_protocol: GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.

data_processing: The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.

label: biotin

organism: Homo sapiens

label_protocol: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).

growth_protocol: SH-SY5Y cells were grown to confluence and starved overnight in serum-free medium in order to silence any sustained effects from growth factor signaling.

extract_protocol: Total RNA was isolated 4 hours after PDGF addition. Experiments were performed in duplicate. Trizol extraction of total RNA was performed according to the manufacturer's instructions.

tag: SH-SY5Y neuroblastoma cells


title: Apigenin_PDGF_4_hours, biological rep2

description: Gene expression data from SH-SY5Y cells treated with 74 mM Apigenin for 1h, then with 50ng/ml PDGF for 4h.


title: Nothing_3_hours, biological rep2

description: Gene expression data from SH-SY5Y cells treated with DMSO for 1h, then with water for 3h.

extract_protocol: Total RNA was isolated 3 hours after PDGF addition. Experiments were performed in duplicate. Trizol extraction of total RNA was performed according to the manufacturer's instructions.


title: U0126_PDGF_4_hours, biological rep2

description: Gene expression data from SH-SY5Y cells treated with 50 mM U0126 for 1h, then with 50ng/ml PDGF for 4h.


title: Nothing_4_hours, biological rep1

source_name: SH-SY5Y cells treated with DMSO for 1h, then with water for 4h.


title: MyoD-/-, P10, 0hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S343
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_352
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 0 hr
Estimated purity:				100%
RNA concentration:			1468.0 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL


title: MyoD-/-, P6, 0hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S343
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_352
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 0 hr
Estimated purity:				100%
RNA concentration:			 4327.4 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL


title: PDGF_4_hours, biological rep2

description: Gene expression data from SH-SY5Y cells treated with DMSO for 1h, then with 50ng/ml PDGF for 4h.


title: MyoD, wt841, 7 days, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S342
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_351
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 7 days
Estimated purity:				100%
RNA concentration:			1142.0 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6 Balb/c
Sample volume:				27µL


title: Nothing_3_hours, biological rep1

source_name: SH-SY5Y cells treated with DMSO for 1h, then with water for 3h.


title: MyoD-/-, 1999, 0hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S343
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_352
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 0 hr
Estimated purity:				100%
RNA concentration:			3325.8 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL


title: U0126_PDGF_4_hours, biological rep1

source_name: SH-SY5Y cells treated with 50 mM U0126 for 1h, then with 50ng/ml PDGF for 4h.


title: MyoD, mb1, 7 days, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S342
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_351
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 7 days
Estimated purity:				100%
Num cells for RNA prep:			More than 2 x 10E+6 Balb/c


title: PDGF_4_hours, biological rep1

source_name: SH-SY5Y cells treated with DMSO for 1h, then with 50ng/ml PDGF for 4h.


title: Apigenin_PDGF_4_hours, biological rep1

source_name: SH-SY5Y cells treated with 74 mM Apigenin for 1h, then with 50ng/ml PDGF for 4h.


title: U0126_PDGF_3_hours, biological rep2

description: Gene expression data from SH-SY5Y cells treated with 50 mM U0126 for 1h, then with 50ng/ml PDGF for 3h.


title: MyoD, wt841, 4 days, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S341
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_350
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 4 days
Estimated purity:				100%
RNA concentration:			2370.8 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6 Balb/c
Sample volume:				27µL


title: Apigenin_PDGF_3_hours, biological rep1

description: Gene expression data from SH-SY5Y cells treated with 74 mM Apigenin for 1h, then with 50ng/ml PDGF for 3h.


title: Apigenin_PDGF_3_hours, biological rep2

source_name: SH-SY5Y cells treated with 74 mM Apigenin for 1h, then with 50ng/ml PDGF for 3h.


title: PDGF_3_hours, biological rep2

description: Gene expression data from SH-SY5Y cells treated with DMSO for 1h, then with 50ng/ml PDGF for 3h.


title: U0126_PDGF_3_hours, biological rep1

source_name: SH-SY5Y cells treated with 50 mM U0126 for 1h, then with 50ng/ml PDGF for 3h.


title: MyoD, wtp16, 7 days, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S342
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_351
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 7 days
Estimated purity:				100%
RNA concentration:			1637.2 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6 Balb/c
Sample volume:				27µL


title: MyoD, wt841, 3 days, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S340
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_349
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 3 days
Estimated purity:				100%
RNA concentration:			1467.8 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6 Balb/c
Sample volume:				27µL


title: MyoD, mb1, 4 days, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S341
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_350
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 4 days
Estimated purity:				100%
Num cells for RNA prep:			More than 2 x 10E+6 Balb/c


title: PDGF_3_hours, biological rep1

source_name: SH-SY5Y cells treated with DMSO for 1h, then with 50ng/ml PDGF for 3h.


title: MyoD, wt841,  48hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S339
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_348
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 48 hr
Estimated purity:				100%
RNA concentration:			1879.0 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6 Balb/c
Sample volume:				27µL


title: MyoD, mb1, 3 days, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S340
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_349
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 3 days
Estimated purity:				100%
Num cells for RNA prep:			More than 2 x 10E+6 Balb/c


title: MyoD, wtp16, 36hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S338
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_347
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 36 hr
Estimated purity:				100%
RNA concentration:			2482.4 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6 Balb/c
Sample volume:				27µL


title: Nothing_2_hours, biological rep1

description: Gene expression data from SH-SY5Y cells treated with DMSO for 1h, then with water for 2h.

extract_protocol: Total RNA was isolated 2 hours after PDGF addition. Experiments were performed in duplicate. Trizol extraction of total RNA was performed according to the manufacturer's instructions.


title: Apigenin_PDGF_2_hours, biological rep2

description: Gene expression data from SH-SY5Y cells treated with 74 mM Apigenin for 1h, then with 50ng/ml PDGF for 2h.


title: U0126_PDGF_2_hours, biological rep1

description: Gene expression data from SH-SY5Y cells treated with 50 mM U0126 for 1h, then with 50ng/ml PDGF for 2h.


title: MyoD, wtp16,  48hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S339
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_348
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 48 hr
Estimated purity:				100%
RNA concentration:			1724.0 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6 Balb/c
Sample volume:				27µL


title: MyoD, wt841, 36hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S338
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_347
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 36 hr
Estimated purity:				100%
RNA concentration:			2726.2 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6 Balb/c
Sample volume:				27µL


title: Nothing_2_hours, biological rep2

source_name: SH-SY5Y cells treated with DMSO for 1h, then with water for 2h.


title: Apigenin_PDGF_2_hours, biological rep1

source_name: SH-SY5Y cells treated with 74 mM Apigenin for 1h, then with 50ng/ml PDGF for 2h.


title: U0126_PDGF_2_hours, biological rep2

source_name: SH-SY5Y cells treated with 50 mM U0126 for 1h, then with 50ng/ml PDGF for 2h.


title: MyoD, wtp16, 3 days, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S340
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_349
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 3 days
Estimated purity:				100%
RNA concentration:			1587.6 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6 Balb/c
Sample volume:				27µL


title: PDGF_2_hours, biological rep2

description: Gene expression data from SH-SY5Y cells treated with DMSO for 1h, then with 50ng/ml PDGF for 2h.


title: PDGF_2_hours, biological rep1

source_name: SH-SY5Y cells treated with DMSO for 1h, then with 50ng/ml PDGF for 2h.


title: MyoD, mb1,  48hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S339
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_348
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 48 hr
Estimated purity:				100%
Num cells for RNA prep:			More than 2 x 10E+6 Balb/c


title: MyoD, mb1, 36hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S338
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_347
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 36 hr
Estimated purity:				100%
Num cells for RNA prep:			More than 2 x 10E+6 Balb/c


title: MyoD, wtp16, 4 days, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S341
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_350
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 4 days
Estimated purity:				100%
RNA concentration:			1166.8 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6 Balb/c
Sample volume:				27µL


title: Apigenin_PDGF_1_hour, biological rep1

description: Gene expression data from SH-SY5Y cells treated with 74 mM Apigenin for 1h, then with 50ng/ml PDGF for 1h.

extract_protocol: Total RNA was isolated 1 hour after PDGF addition. Experiments were performed in duplicate. Trizol extraction of total RNA was performed according to the manufacturer's instructions.


title: U0126_PDGF_1_hour, biological rep2

description: Gene expression data from SH-SY5Y cells treated with 50 mM U0126 for 1h, then with 50ng/ml PDGF for 1h.


title: MyoD, wt841, 24hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S337
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_346
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 24 hr
Estimated purity:				100%
RNA concentration:			1830.8 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6 Balb/c
Sample volume:				27µL


title: Apigenin_PDGF_1_hour, biological rep2

source_name: SH-SY5Y cells treated with 74 mM Apigenin for 1h, then with 50ng/ml PDGF for 1h.


title: Nothing_1_hour, biological rep1

description: Gene expression data from SH-SY5Y cells treated with DMSO for 1h, then with water for 1h.


title: MyoD, wt841, 18hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S336
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_345
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 18 hr
Estimated purity:				100%
RNA concentration:			1597.2 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6 Balb/c
Sample volume:				27µL


title: MyoD, mb1,  24hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S337
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_346
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 24 hr
Estimated purity:				100%
Num cells for RNA prep:			   More than 2 x 10E+6 Balb/c


title: U0126_PDGF_1_hour, biological rep1

source_name: SH-SY5Y cells treated with 50 mM U0126 for 1h, then with 50ng/ml PDGF for 1h.


title: MyoD, wtp16, 18hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S336
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_345
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 18 hr
Estimated purity:				100%
RNA concentration:			1593.0 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6 Balb/c
Sample volume:				27µL


title: MyoD, mb1, 18hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S336
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_345
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 18 hr
Estimated purity:				100%
Num cells for RNA prep:			More than 2 x 10E+6 Balb/c


title: PDGF_1_hour, biological rep2

description: Gene expression data from SH-SY5Y cells treated with DMSO for 1h, then with 50ng/ml PDGF for 1h.


title: MyoD, wtp16, 24hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S337
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_346
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 24 hr
Estimated purity:				100%
RNA concentration:			1624.4 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6 Balb/c
Sample volume:				27µL


title: Apigenin_PDGF_half_hour, biological rep1

description: Gene expression data from SH-SY5Y cells treated with 74 mM Apigenin for 1h, then with 50ng/ml PDGF for 0.5h.

extract_protocol: Total RNA was isolated 0.5 hours after PDGF addition. Experiments were performed in duplicate. Trizol extraction of total RNA was performed according to the manufacturer's instructions.


title: PDGF_1_hour, biological rep1

source_name: SH-SY5Y cells treated with DMSO for 1h, then with 50ng/ml PDGF for 1h.


title: Nothing_half_hour, biological rep1

description: Gene expression data from SH-SY5Y cells treated with DMSO for 1h, then with water for 0.5h.


title: MyoD, mb1, 12hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S335
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_344
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time course: 12 hr
Estimated purity:				100%
Num cells for RNA prep:			More than 2 x 10E+6 Balb/c


title: MyoD, wt841, 0hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S333
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_342
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time course: 0 hr
Estimated purity:				100%
RNA concentration:			2297.8 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6   Balb/c
Sample volume:				27µL


title: Nothing_half_hour, biological rep2

source_name: SH-SY5Y cells treated with DMSO for 1h, then with water for 0.5h.


title: Apigenin_PDGF_half_hour, biological rep2

source_name: SH-SY5Y cells treated with 74 mM Apigenin for 1h, then with 50ng/ml PDGF for 0.5h.


title: MyoD, wtp16, 6hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S334
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_343
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time course: 6 hr
Estimated purity:				100%
RNA concentration:			1393.0 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6   Balb/c
Sample volume:				27µL

source_name: Primary Myoblasts, Skeletal Muscle


title: U0126_PDGF_half_hour, biological rep2

description: Gene expression data from SH-SY5Y cells treated with 50 mM U0126 for 1h, then with 50ng/ml PDGF for 0.5h.


title: MyoD, wt841, 12hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S335
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_344
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time course: 12 hr
Estimated purity:				100%
RNA concentration:			2012.8 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6 Balb/c
Sample volume:				27µL


title: MyoD, mb1, 6hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S334
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_343
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time course: 6 hr
Estimated purity:				100%
Num cells for RNA prep:			More than 2 x 10E+6   Balb/c


title: U0126_PDGF_half_hour, biological rep1

source_name: SH-SY5Y cells treated with 50 mM U0126 for 1h, then with 50ng/ml PDGF for 0.5h.


title: MyoD, wtp16, 12hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S335
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_344
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time course: 12 hr
Estimated purity:				100%
RNA concentration:			1760.6 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6 Balb/c
Sample volume:				27µL


title: MyoD-/-,P6, 7d, chip MG_U74Bv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S352
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_361
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 7 day
Estimated purity:				100%
RNA concentration:			2347.2 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL


title: MyoD-/-,P10, 7d, chip MG_U74Bv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S352
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_361
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 7 day
Estimated purity:				100%
RNA concentration:			1545.2 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL


title: PDGF_half_hour, biological rep2

description: Gene expression data from SH-SY5Y cells treated with DMSO for 1h, then with 50ng/ml PDGF for 0.5h.


title: PDGF_half_hour, biological rep1

source_name: SH-SY5Y cells treated with DMSO for 1h, then with 50ng/ml PDGF for 0.5h.


title: MyoD, mb1, 0hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S333
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_342
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time course: 0 hr
Estimated purity:				100%
Num cells for RNA prep:			More than 2 x 10E+6   Balb/c


title: MyoD-/-,1999, 7d, chip MG_U74Bv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S352
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_361
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 7 day
Estimated purity:				100%
RNA concentration:			1374.0 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL


title: MyoD, wt841, 6hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S334
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_343
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time course: 6 hr
Estimated purity:				100%
RNA concentration:			1212.4 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6   Balb/c
Sample volume:				27µL


title: MyoD-/-, P6, 4d, chip MG_U74Bv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S351
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_360
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 4 day
Estimated purity:				100%
RNA concentration:			2485.4 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL


title: MyoD-/-, P10, 4d, chip MG_U74Bv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S351
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_360
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 4 day
Estimated purity:				100%
RNA concentration:			1130.3 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL


title: MyoD-/-, P10, 3d, chip MG_U74Bv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S350
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_359
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 3 day
Estimated purity:				100%
RNA concentration:			1336.7 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL


title: MyoD-/-,P6, 3d, chip MG_U74Bv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S350
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_359
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 3 day
Estimated purity:				100%
RNA concentration:			1652.8 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL


title: MyoD-/-,1999, 3d, chip MG_U74Bv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S350
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_359
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 3 day
Estimated purity:				100%
RNA concentration:			1937.6 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL


title: MyoD, wtp16, 0hr, chip MG_U74Cv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E233
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=233
SCGP Sample ID:				S333
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=233#SAMPLE_342
Short description:			Time Course in vitro Differentiation of Myogenic Primary Myoblast into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time course: 0 hr
Estimated purity:				100%
RNA concentration:			1170.2 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6   Balb/c
Sample volume:				27µL


title: MyoD-/-, P10, 48hr, chip MG_U74Bv2

description:
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca).  The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID:			E234
Stembase Experiment ID link:		http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID:				S349
SCGP Sample ID link:			http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_358
Short description:			Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 48 hr
Estimated purity:				100%
RNA concentration:			1294.3 ng/µL
Num cells for RNA prep:			More than 2 x 10E+6
Sample volume:				27µL