title: CD138_selected_plasma_cells_presentation_MM085


title: CD138_selected_plasma_cells_presentation_MM101

status: Public on Mar 22 2010

type: RNA

city: Sutton

first: Brian

middle: A

last: Walker

address: 15 Cotswold Road

department: Haemato-Oncology Research Unit

country: United Kingdom

institute: The Institute of Cancer Research

description: pre-treatment bone marrow plasma cells selected for CD138+ using microbeads and magnet-assisted cell sorting (MACS; Miltenyi Biotech, Bergisch Gladbach, Germany), according to the manufacturer's instructions.

hyb_protocol: Affymetrix Fluidics Station Protocol: Hybridization-EukGE-WS2v5_450, 15ug of fragmented cRNA were hybridised to U133 Plus 2.0 arrays using the standard Affymetrix conditions for 16 hours at 45C.

scan_protocol: Genechips were scanned on an Affymetrix GeneChip 3000 7G/4 color early acces scanner and processed using GCOS 1.4.0.

data_processing: The data were analyzed with dChip (April 2007 build) using default analysis settings for normalization and summarization methods.

label: biotin

molecule: total RNA

organism: Homo sapiens

source_name: pre-treatment bone marrow

label_protocol: 100 ng of RNA was used in the Affymetrix 2-cycle IVT reaction and cRNA was labelled with biointylated rUTP during the second IVT reaction as per Affymetrix protocol.

extract_protocol: Sample was CD138 positively selected using MACS beads and stored in Trizol, RNA was isolated using Trizol and the Invitrogen Micro-to-Midi total RNA purification kit as per manufacturer's instructions.

tag: tissue

value: myeloma cells

tag: extraction

value: Trizol

tag: storage


title: high.30028

city: Ottawa

first: Andrew

last: Williams

state: ON

address: 50 Columbine

country: Canada

institute: Health Canada

description:
Stratagene, La Jolla, CA, USA
high scan
raw data files: ArrayNumber_PMT_ScanID_Dye.txt, PMT is % of maximum power

hyb_protocol: Agilent Mouse G4121A Microarrays (containing approximately 22,000 probes) were hybridized with 5 µg Cy3-labelled Universal Mouse Reference RNA (Stratagene, CA, USA). Arrays were incubated overnight at 60 ºC in Agilent hybridization solution and washed according to manufacturer’s instructions.

scan_protocol: Arrays were scanned using a ScanArray Express (Perkin-Elmer Life Sciences, Woodbridge, ON, Canada), and data were acquired with ImaGene 5.5 (BioDiscovery, CA, USA).

data_processing: The median signal intensities were normalized by cyclic lowess.

organism: Mus musculus

source_name: universal mouse reference total RNA (Catalog #740100; Stratagene, La Jolla, CA, USA)

label_protocol: Individual 2.5 µg aliquots of RNA from each sample were amplified and labelled using the Low RNA Input Fluorescent Linear Amplification Kit (Agilent).

extract_protocol:
purchased from Stratagene
Unknown: Material was purchased as total RNA (universal mouse reference total RNA (Stratagene, CA, USA))

tag: sample type

value: Total RNA derived from 11 mouse cell lines

tag: id

tag: set

value: Hybridization Block 6


title: high.30029

value: Hybridization Block 7


title: CD138_selected_plasma_cells_presentation_MM095

extract_protocol: Sample was CD138 positively selected using MACS beads and stored in RLT, extracted using Qiagen AllPrep DNA/RNA mini kit as per manufacturer's protocol.

value: AllPrep

value: RLT


title: medium.30028

description:
Stratagene, La Jolla, CA, USA
medium scan
raw data files: ArrayNumber_PMT_ScanID_Dye.txt, PMT is % of maximum power


title: low.30172

description:
Stratagene, La Jolla, CA, USA
low scan
raw data files: ArrayNumber_PMT_ScanID_Dye.txt, PMT is % of maximum power

value: Hybridization Block 5


title: khondoker3Scan.30027

description:
Stratagene, La Jolla, CA, USA
khondoker3Scan
raw data files: ArrayNumber_PMT_ScanID_Dye.txt, PMT is % of maximum power


title: CD138_selected_plasma_cells_presentation_MM097


title: CD138_selected_plasma_cells_presentation_MM084


title: garcia.30027

description:
Stratagene, La Jolla, CA, USA
garcia scan
raw data files: ArrayNumber_PMT_ScanID_Dye.txt, PMT is % of maximum power


title: low.30028


title: khondoker2Scan.30027

description:
Stratagene, La Jolla, CA, USA
khondoker2Scan
raw data files: ArrayNumber_PMT_ScanID_Dye.txt, PMT is % of maximum power


title: lyng.30027

description:
Stratagene, La Jolla, CA, USA
lyng scan
raw data files: ArrayNumber_PMT_ScanID_Dye.txt, PMT is % of maximum power


title: CD138_selected_plasma_cells_presentation_MM083


title: low.30027


title: CD138_selected_plasma_cells_presentation_MM076


title: low.30029


title: CD138_selected_plasma_cells_presentation_MM077


title: CD138_selected_plasma_cells_presentation_MM082


title: CD138_selected_plasma_cells_presentation_MM081


title: CD138_selected_plasma_cells_presentation_MM079


title: lyng.29918

value: Hybridization Block 3


title: medium.30027


title: CD138_selected_plasma_cells_presentation_MM075


title: khondoker2Scan.29918


title: garcia.29918


title: CD138_selected_plasma_cells_presentation_MM074


title: high.30027


title: CD138_selected_plasma_cells_presentation_MM078


title: khondoker3Scan.29918


title: medium.29918


title: CD138_selected_plasma_cells_presentation_MM080


title: garcia.29917


title: khondoker3Scan.29917


title: low.29918


title: CD138_selected_plasma_cells_presentation_MM073

extract_protocol: Sample was CD138 positively selected using MACS beads and stored in RLT, extracted using Qiagen RNA/DNA kit and nucleic acids extracted as per manufacturer's protocol.

value: RNA/DNA kit


title: CD138_selected_plasma_cells_presentation_MM066


title: CD138_selected_plasma_cells_presentation_MM071


title: CD138_selected_plasma_cells_presentation_MM070


title: high.29918


title: CD138_selected_plasma_cells_presentation_MM069


title: CD138_selected_plasma_cells_presentation_MM065


title: khondoker2Scan.29917


title: lyng.29917


title: CD138_selected_plasma_cells_presentation_MM068


title: CD138_selected_plasma_cells_presentation_MM072


title: high.29917


title: medium.29917


title: CD138_selected_plasma_cells_presentation_MM063


title: CD138_selected_plasma_cells_presentation_MM062


title: khondoker2Scan.29916

value: Hybridization Block 2


title: CD138_selected_plasma_cells_presentation_MM064


title: khondoker3Scan.29916


title: low.29917


title: CD138_selected_plasma_cells_presentation_MM059


title: CD138_selected_plasma_cells_presentation_MM061


title: lyng.29916


title: CD138_selected_plasma_cells_presentation_MM060


title: CD138_selected_plasma_cells_presentation_MM054


title: khondoker3Scan.29915


title: garcia.29916


title: CD138_selected_plasma_cells_presentation_MM056


title: garcia.29915


title: CD138_selected_plasma_cells_presentation_MM057


title: CD138_selected_plasma_cells_presentation_MM058


title: CD138_selected_plasma_cells_presentation_MM052


title: khondoker2Scan.29915


title: CD138_selected_plasma_cells_presentation_MM055


title: medium.29916


title: CD138_selected_plasma_cells_presentation_MM050


title: high.29915


title: CD138_selected_plasma_cells_presentation_MM053


title: high.29916


title: lyng.29915


title: CD138_selected_plasma_cells_presentation_MM049


title: low.29916


title: low.29915


title: khondoker2Scan.29913


title: CD138_selected_plasma_cells_presentation_MM051


title: garcia.29913


title: lyng.29913


title: khondoker3Scan.29913


title: CD138_selected_plasma_cells_presentation_MM048


title: medium.29915


title: CD138_selected_plasma_cells_presentation_MM046


title: CD138_selected_plasma_cells_presentation_MM043


title: CD138_selected_plasma_cells_presentation_MM044


title: CD138_selected_plasma_cells_presentation_MM045


title: low.29913


title: high.29913


title: CD138_selected_plasma_cells_presentation_MM047


title: CD138_selected_plasma_cells_presentation_MM041


title: medium.29913


title: CD138_selected_plasma_cells_presentation_MM039


title: CD138_selected_plasma_cells_presentation_MM042


title: CD138_selected_plasma_cells_presentation_MM040


title: CD138_selected_plasma_cells_presentation_MM038


title: CD138_selected_plasma_cells_presentation_MM037


title: CD138_selected_plasma_cells_presentation_MM036


title: CD138_selected_plasma_cells_presentation_MM035


title: α-amantin-treated BCB two-cell embryos, Sample3

city: Philadelphia

first: Zhiming

last: Han

state: PA

address: 3307 North Broad Street, AHB 561

department: Fels Institute for Cancer Research and Molecular Biology

country: USA

institute: Temple University

hyb_protocol: Standard Affymetrix procedures.

scan_protocol: Standard Affymetrix procedures.

data_processing: Microarray Analysis Suite 5.0 (MAS, Affymetrix) was used to quantify the microarray signals with default analysis parameters and global scaling to target a mean equal to 150 signal units.

source_name: Mouse two-cell embryos, BCB, α-amantin-treated

label_protocol: ENZO RNA labelling kit.

extract_protocol: Picopure RNA extraction kit.

tag: strain of maternal pronucleus

tag: strain of paternal pronucleus

tag: strain of ooplasm

tag: developmental stage

value: two-cell embryos

tag: treatment

value: α-amantin


title: α-amantin-treated BCB two-cell embryos, Sample4


title: α-amantin-treated BCB two-cell embryos, Sample2


title: α-amantin-treated BCB two-cell embryos, Sample1


title: α-amantin-treated BBB two-cell embryos, Sample2

source_name: Mouse two-cell embryos, BBB, α-amantin-treated


title: α-amantin-treated BBB two-cell embryos, Sample4


title: CD138_selected_plasma_cells_presentation_MM034


title: CD138_selected_plasma_cells_presentation_MM021


title: α-amantin-treated BBB two-cell embryos, Sample1


title: CD138_selected_plasma_cells_presentation_MM028


title: CD138_selected_plasma_cells_presentation_MM032


title: CD138_selected_plasma_cells_presentation_MM024


title: α-amantin-treated BBB two-cell embryos, Sample3


title: CD138_selected_plasma_cells_presentation_MM026


title: CD138_selected_plasma_cells_presentation_MM022


title: CD138_selected_plasma_cells_presentation_MM025


title: CD138_selected_plasma_cells_presentation_MM033


title: CD138_selected_plasma_cells_presentation_MM030


title: CD138_selected_plasma_cells_presentation_MM031


title: CD138_selected_plasma_cells_presentation_MM023


title: CD138_selected_plasma_cells_presentation_MM029


title: CD138_selected_plasma_cells_presentation_MM027


title: CD138_selected_plasma_cells_presentation_MM013


title: CD138_selected_plasma_cells_presentation_MM020


title: CD138_selected_plasma_cells_presentation_MM015


title: CD138_selected_plasma_cells_presentation_MM019


title: CD138_selected_plasma_cells_presentation_MM017


title: CD138_selected_plasma_cells_presentation_MM010


title: CD138_selected_plasma_cells_presentation_MM012


title: CD138_selected_plasma_cells_presentation_MM009


title: CD138_selected_plasma_cells_presentation_MM007


title: CD138_selected_plasma_cells_presentation_MM003


title: CD138_selected_plasma_cells_presentation_MM002


title: CD138_selected_plasma_cells_presentation_MM004


title: CD138_selected_plasma_cells_presentation_MM006


title: CD138_selected_plasma_cells_presentation_MM005


title: CD138_selected_plasma_cells_presentation_MM011


title: CD138_selected_plasma_cells_presentation_MM008


title: peripheral_white_blood_cells_presentation_PB384_nsp

type: genomic

hyb_protocol: 90 ug of fragmented labeled DNA is hybridised to the array for 16 hours at 49C accoring to Affymetrix protocols.

data_processing: SNP genotype calls were generated using Affymetrix Powertools  v1.8.0 (apt-probeset-genotype).  Calls were generated using the default parameters and the brlmm calling algorithm.

molecule: genomic DNA

source_name: peripheral white blood cell pellets

label_protocol: 250 ng of DNA is digested with NspI/StyI, and processed accorodng to Affymetrix 500K protocol.  DNA is end-labeled with biotin using TdT.

extract_protocol: DNA was extracted from peripheral white blood cell pellets using the Qiagen Flexigene kit, as per manufacturer's instructions.

value: Flexigene


title: CD138_selected_plasma_cells_presentation_MM001


title: peripheral_white_blood_cells_presentation_PB383_nsp


title: peripheral_white_blood_cells_presentation_PB382_nsp


title: Menopur 3

city: Vermillion

first: Kathleen

middle: M

last: Eyster

state: SD

address: 414 E. Clark St.

department: Basic Biomedical Sciences

institute: University of South Dakota

description: Biological replicate 3 of 3.  Institutional Review Board approval was obtained for this experiment, and all participants signed informed consent.

hyb_protocol: Hybridization procedures and parameters: 10 micrograms of cRNA was mixed with fragmentation buffer and heated to 94C for 20 min. The fragmented cRNA was mixed with CodeLink hybridization buffer, loaded on the microarray slides, and hybridized for 18 hours at 37C. The slides were washed in 0.75x TNT (Tris-HCl, NaCl, Tween-20) at 46C for 1h then incubated with streptavidin-Alexa 647 fluorescent dye for 30 min at room temperature. The Alexa fluor was prepared in TNB blocking buffer (0.1M Tris-HCl, 0.15M NaCL, 0.5% NEN Blocking Reagent-PerkinElmer) The slides were then washed 4 times for 5 min each in 1x TNT and once in 0.05% Tween 20 for 5 sec. The slides were dried by centrifugation and scanned in an Axon GenePix 4000B scanner.

scan_protocol: Slides were scanned in an Axon GenePix 4000B scanner.

data_processing: global median normalization, CodeLink Expression Analysis 4.1.0.4163

source_name: human granulosa cells

label_protocol: Labelled cRNA was prepared using the manufacturer's Instruction Protocol (www1.amershambiosciences.com, CodeLink Gene Expression System: Manual Labelled cRNA Target Preparation). The source of reagents was the CodeLink Expression Assay Reagent Kit, Manual Prep, except where specified otherwise. 0.285 micrograms of total granulosa cell RNA was mixed with bacterial control RNA spikes and primed with T7 oligo(dT) primer for 10 min at 70C. (The bacterial control spikes included araB, entF, fixB, gnd, hisB, and leuB.) The first strand of cDNA was synthesized with first strand buffer, dNTP mix, RNase inhibitor, and reverse transcriptase for 2 h at 42C. The second strand cDNA synthesis reaction was prepared using second strand buffer, dNTP mix, DNA polymerase mix, and RNase H; the reaction was carried out for 2h at 16C. The double-stranded cDNA was purified on QIAquick columns (Qiagen) and the eluent was dried down in a SpeedVac concentrator. The double-stranded cDNA was resuspended in a mixture containing T7 reaction buffer, T7 ATP, T7 GTP, T7 UTP, T7 CTP, biotin-11-UTP, and T7 enzyme mix for the synthesis of cRNA. The cRNA synthesis reaction was terminated after 14h at 37C by purifying the cRNA on RNeasy columns (Qiagen). The concentration of cRNA was determined by spectrophotometry.

extract_protocol: The granulosa cells were collected into RNAlater (Ambion), washed, centrifuged over 40% Percoll, resuspended in RNAlater, and snap-frozen in liquid nitrogen. For RNA extraction, the granulosa cells were centrifuged out of RNAlater.  The cell pellet was resuspended in 1 ml TRI reagent (MRC) and homogenized.  Bromochloropropane and sodium acetate were added to the homogenate and the sample was centrifuged to separate the phases.  The RNA-containing layer was removed and the RNA purified on an RNeasy extraction column (Qiagen).  The sample was treated with an on-column DNase treatment (RNase-free DNase, Qiagen).  The purity and quantity of the total RNA was evaluated with the RNA 6000 NanoLabChip in an Agilent Bioanalyzer.

value: human granulosa cells, gender:female, age:less than 35 years, species:human, treatment:purified human menopausal gonadotropin


title: peripheral_white_blood_cells_presentation_PB381_nsp


title: peripheral_white_blood_cells_presentation_PB374_nsp


title: Gonal 4

description: Biological replicate 4 of 4.  Institutional Review Board approval was obtained for this experiment, and all participants signed informed consent.

value: human granulosa cells, gender:female, age:less than 35 years, species:human, treatment:recombinant human follicle stimulating hormone


title: Menopur 2

description: Biological replicate 2 of 3.  Institutional Review Board approval was obtained for this experiment, and all participants signed informed consent.


title: Menopur 1

description: Biological replicate 1 of 3.  Institutional Review Board approval was obtained for this experiment, and all participants signed informed consent.


title: Gonal 2

description: Biological replicate 2 of 4.  Institutional Review Board approval was obtained for this experiment, and all participants signed informed consent.


title: Gonal 1

description: Biological replicate 1 of 4.  Institutional Review Board approval was obtained for this experiment, and all participants signed informed consent.


title: Gonal 3

description: Biological replicate 3 of 4.  Institutional Review Board approval was obtained for this experiment, and all participants signed informed consent.


title: peripheral_white_blood_cells_presentation_PB377_nsp


title: peripheral_white_blood_cells_presentation_PB373_nsp


title: peripheral_white_blood_cells_presentation_PB372_nsp


title: peripheral_white_blood_cells_presentation_PB380_nsp


title: peripheral_white_blood_cells_presentation_PB379_nsp


title: Monocyte T02

city: London

first: Simon

last: Waddell

department: Division of Cellular and Molecular Medicine St. George's

institute: University of London Tooting

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58682.gif

hyb_protocol: not provided

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 280, 4950; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-11; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 530; Parameter Laser on time = 212951; Parameter Laser Power = 3.57; Parameter Scan Time = 17:17:21; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.16; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-11; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 670; Parameter Scan Power = 100; Parameter Laser on time = 431954; Parameter Laser Power = 2.55; Parameter Scan Time = 17:17:21; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.16; Performer: Simon,,Waddell

data_processing: VALUE is Log (base 2) of the ratio of the median of Channel 2 (usually 635 nm) to Channel 1 (usually 532 nm)

source_name: Stratagene 1 Round Amplification 3 ug

label_protocol: not provided

extract_protocol: not provided

tag: reference

value: Stratagene 1 Round Amplification 3 ug

source_name: 4ug Amplified Monocyte T02

tag: measurement

value: time(absolute) 0 h

tag: compound based treatment

value: Mock

tag: cell type

value: Monocyte


title: NK Cell T01

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58666.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 720, 5760; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-11; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 590; Parameter Laser on time = 212717; Parameter Laser Power = 2.98; Parameter Scan Time = 13:22:15; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 32.22; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-11; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 720; Parameter Scan Power = 100; Parameter Laser on time = 431720; Parameter Laser Power = 2.51; Parameter Scan Time = 13:22:15; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 32.22; Performer: Simon,,Waddell

source_name: 4ug Amplified NK Cell T01

value: NK Cell


title: NK Cell 0.6 pM IFNg 24 h Repeat

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58835.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 960, 5800; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-21; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 560; Parameter Laser on time = 214297; Parameter Laser Power = 3.33; Parameter Scan Time = 16:33:59; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 32.71; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-21; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 610; Parameter Scan Power = 100; Parameter Laser on time = 433303; Parameter Laser Power = 2.49; Parameter Scan Time = 16:33:59; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 32.71; Performer: Simon,,Waddell

source_name: 4ug Amplified NK Cell 0.6 pM IFNg 24 h Repeat

value: time(absolute) 24 h

value: 0.6 pM IFNg


title: peripheral_white_blood_cells_presentation_PB378_nsp


title: peripheral_white_blood_cells_presentation_PB369_nsp


title: peripheral_white_blood_cells_presentation_PB375_nsp


title: peripheral_white_blood_cells_presentation_PB376_nsp


title: peripheral_white_blood_cells_presentation_PB371_nsp


title: peripheral_white_blood_cells_presentation_PB370_nsp


title: B Cell 0.6 pM IFNg 1 h

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58661.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 580, 4750; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-10; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 550; Parameter Laser on time = 212317; Parameter Laser Power = 3.46; Parameter Scan Time = 00:41:17; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.53; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-10; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 700; Parameter Scan Power = 100; Parameter Laser on time = 431320; Parameter Laser Power = 2.56; Parameter Scan Time = 00:41:17; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.53; Performer: Simon,,Waddell

source_name: 4ug Amplified B Cell 0.6 pM IFNg 1 h

value: time(absolute) 1 h

value: B Cell


title: peripheral_white_blood_cells_presentation_PB383_sty


title: peripheral_white_blood_cells_presentation_PB380_sty


title: peripheral_white_blood_cells_presentation_PB378_sty


title: peripheral_white_blood_cells_presentation_PB377_sty


title: peripheral_white_blood_cells_presentation_PB384_sty


title: peripheral_white_blood_cells_presentation_PB379_sty


title: peripheral_white_blood_cells_presentation_PB376_sty


title: peripheral_white_blood_cells_presentation_PB382_sty


title: peripheral_white_blood_cells_presentation_PB375_sty


title: peripheral_white_blood_cells_presentation_PB381_sty


title: peripheral_white_blood_cells_presentation_PB368_nsp


title: CD8 Mock 8 h

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58644.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 580, 4750; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 560; Parameter Laser on time = 212122; Parameter Laser Power = 3.70; Parameter Scan Time = 21:23:51; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.50; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 700; Parameter Scan Power = 100; Parameter Laser on time = 431124; Parameter Laser Power = 2.54; Parameter Scan Time = 21:23:51; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.50; Performer: Simon,,Waddell

source_name: 4ug Amplified CD8 Mock 8 h

value: time(absolute) 8 h

value: CD8 Cell


title: peripheral_white_blood_cells_presentation_PB373_sty


title: peripheral_white_blood_cells_presentation_PB372_sty


title: NK Cell T02 Repeat

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58810.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 1440, 5800; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-21; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 550; Parameter Laser on time = 214202; Parameter Laser Power = 2.74; Parameter Scan Time = 14:59:02; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 31.85; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-21; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 590; Parameter Scan Power = 100; Parameter Laser on time = 433208; Parameter Laser Power = 2.49; Parameter Scan Time = 14:59:02; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 31.85; Performer: Simon,,Waddell

source_name: 4ug Amplified NK Cell T02 Repeat


title: peripheral_white_blood_cells_presentation_PB374_sty


title: Monocyte 0.6 pM IFNg 8 h

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58693.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 170, 4550; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-11; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 560; Parameter Laser on time = 213086; Parameter Laser Power = 3.43; Parameter Scan Time = 19:32:41; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.47; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-11; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 660; Parameter Scan Power = 100; Parameter Laser on time = 432089; Parameter Laser Power = 2.57; Parameter Scan Time = 19:32:41; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.47; Performer: Simon,,Waddell

source_name: 4ug Amplified Monocyte 0.6 pM IFNg 8 h


title: peripheral_white_blood_cells_presentation_PB367_nsp


title: peripheral_white_blood_cells_presentation_PB364_nsp


title: CD4 Mock 4 h

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58627.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 760, 5720; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 560; Parameter Laser on time = 211923; Parameter Laser Power = 3.93; Parameter Scan Time = 18:04:40; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.32; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 690; Parameter Scan Power = 100; Parameter Laser on time = 430926; Parameter Laser Power = 2.52; Parameter Scan Time = 18:04:40; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.32; Performer: Simon,,Waddell

source_name: 4ug Amplified CD4 Mock 4 h

value: time(absolute) 4 h

value: CD4 Cell


title: B Cell 0.6 pM IFNg 4 h

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58662.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 580, 4750; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-10; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 550; Parameter Laser on time = 212329; Parameter Laser Power = 3.46; Parameter Scan Time = 00:53:11; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.56; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-10; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 700; Parameter Scan Power = 100; Parameter Laser on time = 431332; Parameter Laser Power = 2.56; Parameter Scan Time = 00:53:11; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.56; Performer: Simon,,Waddell

source_name: 4ug Amplified B Cell 0.6 pM IFNg 4 h


title: NK Cell Mock 24 h

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58674.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 580, 5310; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-11; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 550; Parameter Laser on time = 212846; Parameter Laser Power = 3.52; Parameter Scan Time = 15:32:44; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 32.80; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-11; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 690; Parameter Scan Power = 100; Parameter Laser on time = 431849; Parameter Laser Power = 2.53; Parameter Scan Time = 15:32:44; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 32.80; Performer: Simon,,Waddell

source_name: 4ug Amplified NK Cell Mock 24 h


title: CD8 0.6 pM IFNg 4 h

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58648.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 580, 4750; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 560; Parameter Laser on time = 212168; Parameter Laser Power = 3.65; Parameter Scan Time = 22:11:04; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.53; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 690; Parameter Scan Power = 100; Parameter Laser on time = 431171; Parameter Laser Power = 2.54; Parameter Scan Time = 22:11:04; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.53; Performer: Simon,,Waddell

source_name: 4ug Amplified CD8 0.6 pM IFNg 4 h


title: CD8 0.6 pM IFNg 0.5 h

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58646.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 580, 4750; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 560; Parameter Laser on time = 212145; Parameter Laser Power = 3.64; Parameter Scan Time = 21:47:24; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.56; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 690; Parameter Scan Power = 100; Parameter Laser on time = 431148; Parameter Laser Power = 2.54; Parameter Scan Time = 21:47:24; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.56; Performer: Simon,,Waddell

source_name: 4ug Amplified CD8 0.6 pM IFNg 0.5 h

value: time(absolute) 0.5 h


title: peripheral_white_blood_cells_presentation_PB370_sty

value: Flexigene


title: peripheral_white_blood_cells_presentation_PB371_sty


title: peripheral_white_blood_cells_presentation_PB366_nsp


title: peripheral_white_blood_cells_presentation_PB363_nsp


title: peripheral_white_blood_cells_presentation_PB365_nsp


title: peripheral_white_blood_cells_presentation_PB369_sty


title: peripheral_white_blood_cells_presentation_PB368_sty


title: Monocyte Mock 8 h

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58687.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 280, 4950; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-11; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 540; Parameter Laser on time = 213011; Parameter Laser Power = 3.53; Parameter Scan Time = 18:17:27; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.38; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-11; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 670; Parameter Scan Power = 100; Parameter Laser on time = 432014; Parameter Laser Power = 2.56; Parameter Scan Time = 18:17:27; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.38; Performer: Simon,,Waddell

source_name: 4ug Amplified Monocyte Mock 8 h


title: CD4 0.6 pM IFNg 0.5 h

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58631.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 760, 5720; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 560; Parameter Laser on time = 211969; Parameter Laser Power = 3.85; Parameter Scan Time = 18:50:42; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.44; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 690; Parameter Scan Power = 100; Parameter Laser on time = 430971; Parameter Laser Power = 2.52; Parameter Scan Time = 18:50:42; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.44; Performer: Simon,,Waddell

source_name: 4ug Amplified CD4 0.6 pM IFNg 0.5 h


title: peripheral_white_blood_cells_presentation_PB362_nsp

scan_protocol: Genechips were scanned on an Affymetrix GeneChip 3000 7G/4 color early acces scanner and processed using GCOS 1.4.0.

source_name: peripheral white blood cell pellets


title: peripheral_white_blood_cells_presentation_PB360_nsp


title: peripheral_white_blood_cells_presentation_PB358_nsp


title: peripheral_white_blood_cells_presentation_PB367_sty


title: peripheral_white_blood_cells_presentation_PB359_nsp


title: peripheral_white_blood_cells_presentation_PB364_sty


title: peripheral_white_blood_cells_presentation_PB366_sty


title: peripheral_white_blood_cells_presentation_PB361_nsp


title: NK Cell 0.6 pM IFNg 0.5 h Repeat

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58812.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 1440, 5800; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-21; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 540; Parameter Laser on time = 214229; Parameter Laser Power = 2.52; Parameter Scan Time = 15:26:03; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 32.16; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-21; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 590; Parameter Scan Power = 100; Parameter Laser on time = 433235; Parameter Laser Power = 2.48; Parameter Scan Time = 15:26:03; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 32.16; Performer: Simon,,Waddell

source_name: 4ug Amplified NK Cell 0.6 pM IFNg 0.5 h Repeat


title: peripheral_white_blood_cells_presentation_PB365_sty


title: peripheral_white_blood_cells_presentation_PB361_sty


title: peripheral_white_blood_cells_presentation_PB358_sty


title: peripheral_white_blood_cells_presentation_PB360_sty


title: NK Cell 0.6 pM IFNg 1 h Repeat

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58814.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 1440, 5800; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-21; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 540; Parameter Laser on time = 214243; Parameter Laser Power = 2.95; Parameter Scan Time = 15:40:17; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 32.28; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-21; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 590; Parameter Scan Power = 100; Parameter Laser on time = 433249; Parameter Laser Power = 2.49; Parameter Scan Time = 15:40:17; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 32.28; Performer: Simon,,Waddell

source_name: Stratagene 1 Round Amplification 3 ug

source_name: 4ug Amplified NK Cell 0.6 pM IFNg 1 h Repeat


title: peripheral_white_blood_cells_presentation_PB359_sty


title: peripheral_white_blood_cells_presentation_PB355_nsp


title: peripheral_white_blood_cells_presentation_PB354_sty


title: peripheral_white_blood_cells_presentation_PB352_nsp


title: peripheral_white_blood_cells_presentation_PB353_sty


title: Monocyte 0.6 pM IFNg 1 h

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58691.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 280, 4950; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-11; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 570; Parameter Laser on time = 213060; Parameter Laser Power = 3.47; Parameter Scan Time = 19:06:40; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.47; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-11; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 660; Parameter Scan Power = 100; Parameter Laser on time = 432062; Parameter Laser Power = 2.56; Parameter Scan Time = 19:06:40; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.47; Performer: Simon,,Waddell

source_name: 4ug Amplified Monocyte 0.6 pM IFNg 1 h


title: CD4 Mock 12 h

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58629.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 760, 5720; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 560; Parameter Laser on time = 211945; Parameter Laser Power = 3.90; Parameter Scan Time = 18:27:31; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.32; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 690; Parameter Scan Power = 100; Parameter Laser on time = 430948; Parameter Laser Power = 2.52; Parameter Scan Time = 18:27:31; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.32; Performer: Simon,,Waddell

source_name: 4ug Amplified CD4 Mock 12 h

value: time(absolute) 12 h


title: Monocyte 0.6 pM IFNg 12 h

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58694.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 110, 4250; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-11; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 560; Parameter Laser on time = 213100; Parameter Laser Power = 3.41; Parameter Scan Time = 19:45:56; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.50; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-11; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 660; Parameter Scan Power = 100; Parameter Laser on time = 432103; Parameter Laser Power = 2.57; Parameter Scan Time = 19:45:56; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.50; Performer: Simon,,Waddell

source_name: 4ug Amplified Monocyte 0.6 pM IFNg 12 h


title: CD8 T03

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58639.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 580, 5540; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 560; Parameter Laser on time = 212073; Parameter Laser Power = 3.78; Parameter Scan Time = 20:36:16; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.44; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 690; Parameter Scan Power = 100; Parameter Laser on time = 431076; Parameter Laser Power = 2.53; Parameter Scan Time = 20:36:16; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.44; Performer: Simon,,Waddell

source_name: 4ug Amplified CD8 T03


title: peripheral_white_blood_cells_presentation_PB353_nsp


title: peripheral_white_blood_cells_presentation_PB356_sty


title: Monocyte Mock 1 h

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58685.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 280, 4950; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-11; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 530; Parameter Laser on time = 212987; Parameter Laser Power = 3.60; Parameter Scan Time = 17:53:12; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.32; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-11; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 680; Parameter Scan Power = 100; Parameter Laser on time = 431990; Parameter Laser Power = 2.56; Parameter Scan Time = 17:53:12; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.32; Performer: Simon,,Waddell

source_name: 4ug Amplified Monocyte Mock 1 h


title: NK Cell 0.6 pM IFNg 4 h Repeat

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58820.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 1440, 5800; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-21; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 540; Parameter Laser on time = 214254; Parameter Laser Power = 2.90; Parameter Scan Time = 15:52:01; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 32.34; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-21; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 590; Parameter Scan Power = 100; Parameter Laser on time = 433261; Parameter Laser Power = 2.49; Parameter Scan Time = 15:52:01; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 32.34; Performer: Simon,,Waddell

source_name: 4ug Amplified NK Cell 0.6 pM IFNg 4 h Repeat


title: peripheral_white_blood_cells_presentation_PB348_sty


title: peripheral_white_blood_cells_presentation_PB362_sty


title: peripheral_white_blood_cells_presentation_PB363_sty


title: peripheral_white_blood_cells_presentation_PB351_nsp


title: peripheral_white_blood_cells_presentation_PB351_sty


title: CD4 T01

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58622.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 880, 5640; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 560; Parameter Laser on time = 211749; Parameter Laser Power = 2.87; Parameter Scan Time = 15:10:14; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 32.09; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 700; Parameter Scan Power = 100; Parameter Laser on time = 430751; Parameter Laser Power = 2.50; Parameter Scan Time = 15:10:14; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 32.09; Performer: Simon,,Waddell

source_name: 4ug Amplified CD4 T01


title: peripheral_white_blood_cells_presentation_PB349_nsp


title: peripheral_white_blood_cells_presentation_PB350_nsp


title: peripheral_white_blood_cells_presentation_PB354_nsp


title: peripheral_white_blood_cells_presentation_PB357_sty


title: peripheral_white_blood_cells_presentation_PB350_sty


title: peripheral_white_blood_cells_presentation_PB349_sty


title: Monocyte 0.6 pM IFNg 0.5 h

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58690.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 280, 4950; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-11; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 540; Parameter Laser on time = 213047; Parameter Laser Power = 3.49; Parameter Scan Time = 18:53:45; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.47; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-11; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 670; Parameter Scan Power = 100; Parameter Laser on time = 432050; Parameter Laser Power = 2.56; Parameter Scan Time = 18:53:45; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.47; Performer: Simon,,Waddell

source_name: 4ug Amplified Monocyte 0.6 pM IFNg 0.5 h


title: peripheral_white_blood_cells_presentation_PB347_sty


title: peripheral_white_blood_cells_presentation_PB356_nsp


title: peripheral_white_blood_cells_presentation_PB344_sty


title: NK Cell T03

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58668.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 720, 5760; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-11; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 560; Parameter Laser on time = 212748; Parameter Laser Power = 2.62; Parameter Scan Time = 13:54:02; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 32.43; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-11; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 690; Parameter Scan Power = 100; Parameter Laser on time = 431751; Parameter Laser Power = 2.52; Parameter Scan Time = 13:54:02; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 32.43; Performer: Simon,,Waddell

source_name: 4ug Amplified NK Cell T03


title: peripheral_white_blood_cells_presentation_PB346_sty


title: peripheral_white_blood_cells_presentation_PB345_sty


title: peripheral_white_blood_cells_presentation_PB352_sty


title: CD8 Mock 12 h

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58645.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 580, 4750; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 560; Parameter Laser on time = 212133; Parameter Laser Power = 3.70; Parameter Scan Time = 21:35:38; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.53; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 700; Parameter Scan Power = 100; Parameter Laser on time = 431136; Parameter Laser Power = 2.54; Parameter Scan Time = 21:35:38; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.53; Performer: Simon,,Waddell

source_name: 4ug Amplified CD8 Mock 12 h


title: peripheral_white_blood_cells_presentation_PB357_nsp


title: peripheral_white_blood_cells_presentation_PB347_nsp


title: peripheral_white_blood_cells_presentation_PB343_sty


title: peripheral_white_blood_cells_presentation_PB340_sty


title: peripheral_white_blood_cells_presentation_PB342_sty


title: peripheral_white_blood_cells_presentation_PB355_sty


title: peripheral_white_blood_cells_presentation_PB348_nsp


title: peripheral_white_blood_cells_presentation_PB345_nsp


title: peripheral_white_blood_cells_presentation_PB346_nsp


title: peripheral_white_blood_cells_presentation_PB338_sty


title: peripheral_white_blood_cells_presentation_PB344_nsp


title: CD4 0.6 pM IFNg 1 h

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58632.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 760, 5720; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 560; Parameter Laser on time = 211981; Parameter Laser Power = 3.83; Parameter Scan Time = 19:03:13; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.44; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 680; Parameter Scan Power = 100; Parameter Laser on time = 430984; Parameter Laser Power = 2.52; Parameter Scan Time = 19:03:13; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.44; Performer: Simon,,Waddell

source_name: 4ug Amplified CD4 0.6 pM IFNg 1 h


title: NK Cell T02

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58667.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 720, 5760; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-11; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 580; Parameter Laser on time = 212731; Parameter Laser Power = 3.00; Parameter Scan Time = 13:36:42; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 32.34; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-11; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 700; Parameter Scan Power = 100; Parameter Laser on time = 431734; Parameter Laser Power = 2.51; Parameter Scan Time = 13:36:42; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 32.34; Performer: Simon,,Waddell

source_name: 4ug Amplified NK Cell T02


title: peripheral_white_blood_cells_presentation_PB341_sty


title: peripheral_white_blood_cells_presentation_PB339_sty


title: peripheral_white_blood_cells_presentation_PB343_nsp


title: NK Cell T03 Repeat

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58811.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 1440, 5800; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-21; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 550; Parameter Laser on time = 214214; Parameter Laser Power = 2.89; Parameter Scan Time = 15:11:00; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 31.97; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-21; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 590; Parameter Scan Power = 100; Parameter Laser on time = 433220; Parameter Laser Power = 2.49; Parameter Scan Time = 15:11:00; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 31.97; Performer: Simon,,Waddell

source_name: 4ug Amplified NK Cell T03 Repeat


title: peripheral_white_blood_cells_presentation_PB331_sty

source_name: peripheral white blood cell pellets


title: peripheral_white_blood_cells_presentation_PB340_nsp


title: CD4 Mock 1 h

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58626.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 760, 5720; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 560; Parameter Laser on time = 211910; Parameter Laser Power = 3.94; Parameter Scan Time = 17:52:38; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.29; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 700; Parameter Scan Power = 100; Parameter Laser on time = 430913; Parameter Laser Power = 2.51; Parameter Scan Time = 17:52:38; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.29; Performer: Simon,,Waddell

source_name: 4ug Amplified CD4 Mock 1 h


title: peripheral_white_blood_cells_presentation_PB339_nsp

scan_protocol: Genechips were scanned on an Affymetrix GeneChip 3000 7G/4 color early acces scanner and processed using GCOS 1.4.0.


title: peripheral_white_blood_cells_presentation_PB338_nsp


title: peripheral_white_blood_cells_presentation_PB333_sty


title: CD4 Mock 8 h

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58628.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 760, 5720; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 560; Parameter Laser on time = 211934; Parameter Laser Power = 3.91; Parameter Scan Time = 18:16:06; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.29; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 690; Parameter Scan Power = 100; Parameter Laser on time = 430937; Parameter Laser Power = 2.52; Parameter Scan Time = 18:16:06; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.29; Performer: Simon,,Waddell

source_name: Stratagene 1 Round Amplification 3 ug

source_name: 4ug Amplified CD4 Mock 8 h


title: peripheral_white_blood_cells_presentation_PB334_sty


title: peripheral_white_blood_cells_presentation_PB342_nsp


title: Monocyte 0.6 pM IFNg 4 h

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58692.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 170, 4550; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-11; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 570; Parameter Laser on time = 213072; Parameter Laser Power = 3.44; Parameter Scan Time = 19:18:55; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.47; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-11; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 660; Parameter Scan Power = 100; Parameter Laser on time = 432075; Parameter Laser Power = 2.57; Parameter Scan Time = 19:18:55; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.47; Performer: Simon,,Waddell

source_name: 4ug Amplified Monocyte 0.6 pM IFNg 4 h


title: peripheral_white_blood_cells_presentation_PB341_nsp


title: CD4 Mock 24 h

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58630.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 760, 5720; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 560; Parameter Laser on time = 211956; Parameter Laser Power = 3.87; Parameter Scan Time = 18:38:43; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.35; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 690; Parameter Scan Power = 100; Parameter Laser on time = 430959; Parameter Laser Power = 2.52; Parameter Scan Time = 18:38:43; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.35; Performer: Simon,,Waddell

source_name: 4ug Amplified CD4 Mock 24 h


title: peripheral_white_blood_cells_presentation_PB330_sty


title: peripheral_white_blood_cells_presentation_PB327_sty


title: peripheral_white_blood_cells_presentation_PB332_sty


title: peripheral_white_blood_cells_presentation_PB329_sty


title: B Cell T01

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58651.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 580, 4750; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 560; Parameter Laser on time = 212204; Parameter Laser Power = 3.53; Parameter Scan Time = 22:48:43; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.59; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 670; Parameter Scan Power = 100; Parameter Laser on time = 431207; Parameter Laser Power = 2.55; Parameter Scan Time = 22:48:43; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.59; Performer: Simon,,Waddell

source_name: 4ug Amplified B Cell T01


title: peripheral_white_blood_cells_presentation_PB335_sty


title: peripheral_white_blood_cells_presentation_PB336_sty


title: peripheral_white_blood_cells_presentation_PB324_sty


title: peripheral_white_blood_cells_presentation_PB328_sty


title: peripheral_white_blood_cells_presentation_PB337_nsp


title: peripheral_white_blood_cells_presentation_PB337_sty


title: peripheral_white_blood_cells_presentation_PB326_sty


title: peripheral_white_blood_cells_presentation_PB325_sty


title: peripheral_white_blood_cells_presentation_PB323_sty


title: peripheral_white_blood_cells_presentation_PB336_nsp


title: B Cell T02

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58652.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 580, 4750; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 560; Parameter Laser on time = 212218; Parameter Laser Power = 3.51; Parameter Scan Time = 23:01:35; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.59; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 690; Parameter Scan Power = 100; Parameter Laser on time = 431220; Parameter Laser Power = 2.55; Parameter Scan Time = 23:01:35; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.59; Performer: Simon,,Waddell

source_name: 4ug Amplified B Cell T02


title: CD8 Mock 1 h

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58642.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 580, 4750; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 560; Parameter Laser on time = 212098; Parameter Laser Power = 3.71; Parameter Scan Time = 21:00:30; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.53; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 700; Parameter Scan Power = 100; Parameter Laser on time = 431101; Parameter Laser Power = 2.53; Parameter Scan Time = 21:00:30; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.53; Performer: Simon,,Waddell

source_name: 4ug Amplified CD8 Mock 1 h


title: Monocyte 0.6 pM IFNg 24 h

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58695.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 110, 4250; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-11; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 560; Parameter Laser on time = 213113; Parameter Laser Power = 3.41; Parameter Scan Time = 19:59:54; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.50; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-11; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 670; Parameter Scan Power = 100; Parameter Laser on time = 432116; Parameter Laser Power = 2.57; Parameter Scan Time = 19:59:54; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.50; Performer: Simon,,Waddell

source_name: 4ug Amplified Monocyte 0.6 pM IFNg 24 h


title: NK Cell 0.6 pM IFNg 8 h Repeat

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58830.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 960, 5800; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-21; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 530; Parameter Laser on time = 214267; Parameter Laser Power = 2.75; Parameter Scan Time = 16:04:30; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 32.46; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-21; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 600; Parameter Scan Power = 100; Parameter Laser on time = 433273; Parameter Laser Power = 2.48; Parameter Scan Time = 16:04:30; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 32.46; Performer: Simon,,Waddell

source_name: 4ug Amplified NK Cell 0.6 pM IFNg 8 h Repeat


title: B Cell Mock 1 h

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58655.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 580, 4750; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 550; Parameter Laser on time = 212254; Parameter Laser Power = 3.51; Parameter Scan Time = 23:38:57; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.56; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 700; Parameter Scan Power = 100; Parameter Laser on time = 431257; Parameter Laser Power = 2.56; Parameter Scan Time = 23:38:57; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.56; Performer: Simon,,Waddell

source_name: 4ug Amplified B Cell Mock 1 h


title: peripheral_white_blood_cells_presentation_PB317_sty


title: peripheral_white_blood_cells_presentation_PB318_sty


title: peripheral_white_blood_cells_presentation_PB335_nsp


title: peripheral_white_blood_cells_presentation_PB332_nsp


title: CD8 0.6 pM IFNg 12 h

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58650.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 580, 4750; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 560; Parameter Laser on time = 212193; Parameter Laser Power = 3.60; Parameter Scan Time = 22:35:46; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.56; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 690; Parameter Scan Power = 100; Parameter Laser on time = 431196; Parameter Laser Power = 2.55; Parameter Scan Time = 22:35:46; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.56; Performer: Simon,,Waddell

source_name: 4ug Amplified CD8 0.6 pM IFNg 12 h


title: B Cell Mock 0.5 h

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58654.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 580, 4750; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 560; Parameter Laser on time = 212242; Parameter Laser Power = 3.53; Parameter Scan Time = 23:25:56; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.53; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 700; Parameter Scan Power = 100; Parameter Laser on time = 431245; Parameter Laser Power = 2.55; Parameter Scan Time = 23:25:56; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.53; Performer: Simon,,Waddell

source_name: 4ug Amplified B Cell Mock 0.5 h


title: peripheral_white_blood_cells_presentation_PB333_nsp


title: peripheral_white_blood_cells_presentation_PB316_sty


title: peripheral_white_blood_cells_presentation_PB315_sty


title: B Cell 0.6 pM IFNg 24 h

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58665.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 580, 4750; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-10; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 560; Parameter Laser on time = 212365; Parameter Laser Power = 3.42; Parameter Scan Time = 01:29:28; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.50; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-10; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 700; Parameter Scan Power = 100; Parameter Laser on time = 431368; Parameter Laser Power = 2.56; Parameter Scan Time = 01:29:28; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.50; Performer: Simon,,Waddell

source_name: 4ug Amplified B Cell 0.6 pM IFNg 24 h


title: peripheral_white_blood_cells_presentation_PB314_sty


title: peripheral_white_blood_cells_presentation_PB320_sty


title: peripheral_white_blood_cells_presentation_PB334_nsp


title: peripheral_white_blood_cells_presentation_PB319_sty


title: peripheral_white_blood_cells_presentation_PB311_sty


title: peripheral_white_blood_cells_presentation_PB331_nsp


title: peripheral_white_blood_cells_presentation_PB322_sty


title: CD8 Mock 4 h

description:
Simple annotation: Stimulation, interferon
Image: http://smd.stanford.edu/MicroArray/gifs/2005-01/58643.gif

scan_protocol: Feature Extraction; Software and parameters for feature extraction.; Protocol Type = Feature Extraction; Parameter Datafile type = GenePix Results 3; Parameter Image Origin = 580, 4750; Software: type: feature extraction; Performer: Simon,,Waddell Scanning parameters - Channel 1; Parameters for laser scanning of a hybridized array in channel 1.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Pixel Size = 10; Parameter Scanning software version = 2.0.2.2; Parameter Scan Power = 100; Parameter PMT Volts = 560; Parameter Laser on time = 212109; Parameter Laser Power = 3.71; Parameter Scan Time = 21:12:19; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.50; Performer: Simon,,Waddell Scanning parameters - Channel 2; Parameters for laser scanning of a hybridized array in channel 2.; Protocol Type = Scanning parameters; Parameter Scan Date = 2005-01-09; Parameter Scanning software = AxImageIO; Parameter Scanning software version = 2.0.2.2; Parameter Pixel Size = 10; Parameter PMT Volts = 700; Parameter Scan Power = 100; Parameter Laser on time = 431112; Parameter Laser Power = 2.54; Parameter Scan Time = 21:12:19; Parameter Scanner Make = Axon Instruments (V1.00); Parameter Scanner Model = GenePix 4000B [81971]; Parameter Focus Position = 0; Parameter Scan Temperature = 33.50; Performer: Simon,,Waddell

source_name: 4ug Amplified CD8 Mock 4 h


title: peripheral_white_blood_cells_presentation_PB313_sty


title: peripheral_white_blood_cells_presentation_PB312_sty


title: peripheral_white_blood_cells_presentation_PB310_sty


title: peripheral_white_blood_cells_presentation_PB330_nsp