title: Nasal epithelial cells EB_6_13w_317_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 13w
dose: 6
EB_6_13w_317_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: dilution

tag: bioanalyzer results

tag: sample preservation

tag: strain or line

tag: ncbi taxonomy id

tag: individual


title: Nasal epithelial cells EB_6_13w_320_HCHO_THI08031_Rat230_2_THI 08031

status: Public on Jul 28 2010

type: RNA

city: Research Triangle Park

first: Russell

middle: Scott

last: Thomas

state: NC

address: 6 Davis Drive

country: USA

institute: The Hamner Institutes for Health Sciences

description:
time point: 13w
dose: 6
EB_6_13w_320_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

hyb_protocol: standard Affymetrix procedures

scan_protocol: standard Affymetrix procedures

data_processing: Affymetrix GCOS 1.0, RMA normalization

label: biotin

molecule: total RNA

organism: Rattus norvegicus

source_name: Nasal epithelial cells

label_protocol: standard Affymetrix protocol

extract_protocol: Extraction Method: Qiagen Micro RNA kit

treatment_protocol: in vivo Treatment: Formaldehyde, Treatment Repeat: 5, Units of Treatment Repeat: days per week, Treatment Duration: 13, Units of Duration: weeks, Treatment Time: 6, Units of Treatment Time: hours per day, Route of Exposure: Inhalation (whole-body), Dose or Concentration: 6, Units of Dose or Concentration: ppm

tag: array type

tag: amount to core

tag: units of amount

value: ul

tag: ratio

tag: rna integrity number

value: good

value: Cells suspended in RNAlater at 4 degrees Centigrade for 24 hours and then frozen at -20 degrees Centigrade until processed for RNA.

tag: developmental state

value: Adult

tag: sacrifice method

value: Deep pentobarbital anesthesia followed by exsanguination

tag: sex

value: Male

tag: units of age

value: weeks

tag: age

tag: tissue

value: Nasal epithelial cells (level II region)

tag: tissue harvest methods

value: The nose was dissected to isolate the Level II region of both nostrils.  The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation.  The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood.  After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer.  Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube.  The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX).  The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium.

tag: units of harvest time point

tag: time point

tag: agent

value: formaldehyde

tag: dose


title: Nasal epithelial cells EB_2_5d_266_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 5d
dose: 2
EB_2_5d_266_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

treatment_protocol: in vivo Treatment: Formaldehyde, Treatment Repeat: 5, Units of Treatment Repeat: days per week, Treatment Duration: 5, Units of Duration: days, Treatment Time: 6, Units of Treatment Time: hours per day, Route of Exposure: Inhalation (whole-body), Dose or Concentration: 2, Units of Dose or Concentration: ppm

tag: dilution

tag: bioanalyzer results

tag: sample preservation

tag: strain or line

tag: ncbi taxonomy id

tag: individual

value: days


title: Nasal epithelial cells EB_2_5d_269_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 5d
dose: 2
EB_2_5d_269_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: array type

tag: amount to core

tag: units of amount

tag: rna integrity number

tag: developmental state


title: Nasal epithelial cells EB_6_5d_300_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 5d
dose: 6
EB_6_5d_300_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

treatment_protocol: in vivo Treatment: Formaldehyde, Treatment Repeat: 5, Units of Treatment Repeat: days per week, Treatment Duration: 5, Units of Duration: days, Treatment Time: 6, Units of Treatment Time: hours per day, Route of Exposure: Inhalation (whole-body), Dose or Concentration: 6, Units of Dose or Concentration: ppm

tag: ratio

tag: sacrifice method

tag: sex

tag: units of age

tag: age

tag: tissue


title: Nasal epithelial cells EB_2_13w_287_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 13w
dose: 2
EB_2_13w_287_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

treatment_protocol: in vivo Treatment: Formaldehyde, Treatment Repeat: 5, Units of Treatment Repeat: days per week, Treatment Duration: 13, Units of Duration: weeks, Treatment Time: 6, Units of Treatment Time: hours per day, Route of Exposure: Inhalation (whole-body), Dose or Concentration: 2, Units of Dose or Concentration: ppm

tag: dilution

tag: tissue harvest methods

tag: units of harvest time point

tag: time point

tag: agent

tag: dose


title: Nasal epithelial cells EB_6_4w_307_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 4w
dose: 6
EB_6_4w_307_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: array type

tag: bioanalyzer results

tag: sample preservation

tag: strain or line

tag: ncbi taxonomy id

tag: individual


title: Nasal epithelial cells EB_6_5d_299_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 5d
dose: 6
EB_6_5d_299_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: amount to core

tag: units of amount

tag: ratio

tag: rna integrity number

tag: developmental state

tag: sacrifice method


title: Nasal epithelial cells EB_2_5d_265_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 5d
dose: 2
EB_2_5d_265_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: dilution

tag: sex

tag: units of age

tag: age

tag: tissue

tag: tissue harvest methods


title: Nasal epithelial cells EB_2_4w_276_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 4w
dose: 2
EB_2_4w_276_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: array type

tag: bioanalyzer results

tag: units of harvest time point

tag: time point

tag: agent

tag: dose


title: Nasal epithelial cells EB_15_5d_356_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 5d
dose: 15
EB_15_5d_356_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

treatment_protocol: in vivo Treatment: Formaldehyde, Treatment Repeat: 5, Units of Treatment Repeat: days per week, Treatment Duration: 5, Units of Duration: days, Treatment Time: 6, Units of Treatment Time: hours per day, Route of Exposure: Inhalation (whole-body), Dose or Concentration: 15, Units of Dose or Concentration: ppm

tag: amount to core

tag: units of amount

tag: sample preservation

tag: strain or line

tag: ncbi taxonomy id

tag: individual


title: Nasal epithelial cells EB_2_4w_272_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 4w
dose: 2
EB_2_4w_272_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

treatment_protocol: in vivo Treatment: Formaldehyde, Treatment Repeat: 5, Units of Treatment Repeat: days per week, Treatment Duration: 4, Units of Duration: weeks, Treatment Time: 6, Units of Treatment Time: hours per day, Route of Exposure: Inhalation (whole-body), Dose or Concentration: 2, Units of Dose or Concentration: ppm

tag: ratio

tag: dilution

tag: rna integrity number

tag: developmental state

tag: sacrifice method

tag: sex


title: Nasal epithelial cells EB_2_4w_277_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 4w
dose: 2
EB_2_4w_277_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: array type

tag: bioanalyzer results

tag: units of age

tag: age

tag: tissue

tag: tissue harvest methods


title: Nasal epithelial cells EB_6_13w_316_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 13w
dose: 6
EB_6_13w_316_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: amount to core

tag: units of amount

tag: units of harvest time point

tag: time point

tag: agent

tag: dose


title: Nasal epithelial cells EB_15_5d_354_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 5d
dose: 15
EB_15_5d_354_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: ratio

tag: dilution

tag: sample preservation

tag: strain or line

tag: ncbi taxonomy id

tag: individual


title: Nasal epithelial cells EB_2_4w_273_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 4w
dose: 2
EB_2_4w_273_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: array type

tag: rna integrity number

tag: bioanalyzer results

tag: developmental state

tag: sacrifice method

tag: sex


title: Nasal epithelial cells EB_15_5d_359_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 5d
dose: 15
EB_15_5d_359_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: amount to core

tag: units of amount

tag: units of age

tag: age

tag: tissue

tag: tissue harvest methods


title: Nasal epithelial cells EB_2_13w_290_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 13w
dose: 2
EB_2_13w_290_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: ratio

tag: dilution

tag: units of harvest time point

tag: time point

tag: agent

tag: dose


title: Nasal epithelial cells EB_2_13w_289_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 13w
dose: 2
EB_2_13w_289_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: array type

tag: sample preservation

tag: strain or line

tag: ncbi taxonomy id

tag: individual


title: Nasal epithelial cells EB_2_13w_286_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 13w
dose: 2
EB_2_13w_286_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: amount to core

tag: rna integrity number

tag: bioanalyzer results

tag: developmental state

tag: sacrifice method

tag: sex


title: Nasal epithelial cells EB_15_4w_368_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 4w
dose: 15
EB_15_4w_368_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: units of amount

tag: ratio

tag: units of age

tag: age

tag: tissue

tag: tissue harvest methods


title: Nasal epithelial cells EB_15_5d_358_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 5d
dose: 15
EB_15_5d_358_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: array type

tag: dilution

tag: units of harvest time point

tag: time point

tag: agent

tag: dose


title: Nasal epithelial cells EB_2_5d_268_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 5d
dose: 2
EB_2_5d_268_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: amount to core

tag: rna integrity number

tag: sample preservation

tag: strain or line

tag: ncbi taxonomy id

tag: individual


title: Nasal epithelial cells EB_15_4w_369_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 4w
dose: 15
EB_15_4w_369_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: units of amount

tag: ratio

tag: bioanalyzer results

tag: developmental state

tag: sacrifice method

tag: sex


title: Nasal epithelial cells EB_15_13w_375_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 13w
dose: 15
EB_15_13w_375_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

treatment_protocol: in vivo Treatment: Formaldehyde, Treatment Repeat: 5, Units of Treatment Repeat: days per week, Treatment Duration: 13, Units of Duration: weeks, Treatment Time: 6, Units of Treatment Time: hours per day, Route of Exposure: Inhalation (whole-body), Dose or Concentration: 15, Units of Dose or Concentration: ppm

tag: array type

tag: dilution

tag: units of age

tag: age

tag: tissue

tag: tissue harvest methods


title: Nasal epithelial cells EB_15_13w_377_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 13w
dose: 15
EB_15_13w_377_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: amount to core

tag: units of harvest time point

tag: time point

tag: agent

tag: dose


title: Nasal epithelial cells EB_10_5d_330_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 5d
dose: 10
EB_10_5d_330_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

treatment_protocol: in vivo Treatment: Formaldehyde, Treatment Repeat: 5, Units of Treatment Repeat: days per week, Treatment Duration: 5, Units of Duration: days, Treatment Time: 6, Units of Treatment Time: hours per day, Route of Exposure: Inhalation (whole-body), Dose or Concentration: 10, Units of Dose or Concentration: ppm

tag: units of amount

tag: rna integrity number

tag: sample preservation

tag: strain or line

tag: ncbi taxonomy id

tag: individual


title: Nasal epithelial cells EB_15_13w_378_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 13w
dose: 15
EB_15_13w_378_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: array type

tag: ratio

tag: bioanalyzer results

tag: developmental state

tag: sacrifice method

tag: sex


title: Nasal epithelial cells EB_15_4w_362_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 4w
dose: 15
EB_15_4w_362_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: amount to core

tag: dilution

tag: units of age

tag: age

tag: tissue

tag: tissue harvest methods


title: Nasal epithelial cells EB_15_13w_379_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 13w
dose: 15
EB_15_13w_379_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: units of amount

tag: rna integrity number

tag: units of harvest time point

tag: time point

tag: agent

tag: dose


title: Nasal epithelial cells EB_10_5d_328_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 5d
dose: 10
EB_10_5d_328_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: array type

tag: ratio

tag: sample preservation

tag: strain or line

tag: ncbi taxonomy id

tag: individual


title: Nasal epithelial cells EB_10_5d_324_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 5d
dose: 10
EB_10_5d_324_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: amount to core

tag: dilution

tag: bioanalyzer results

tag: developmental state

tag: sacrifice method

tag: sex


title: Nasal epithelial cells EB_10_5d_329_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 5d
dose: 10
EB_10_5d_329_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: units of amount

tag: rna integrity number

tag: units of age

tag: age

tag: tissue

tag: tissue harvest methods


title: Nasal epithelial cells EB_15_4w_365_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 4w
dose: 15
EB_15_4w_365_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: array type

tag: ratio

tag: units of harvest time point

tag: time point

tag: agent

tag: dose


title: Nasal epithelial cells EB_10_13w_349_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 13w
dose: 10
EB_10_13w_349_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

treatment_protocol: in vivo Treatment: Formaldehyde, Treatment Repeat: 5, Units of Treatment Repeat: days per week, Treatment Duration: 13, Units of Duration: weeks, Treatment Time: 6, Units of Treatment Time: hours per day, Route of Exposure: Inhalation (whole-body), Dose or Concentration: 10, Units of Dose or Concentration: ppm

tag: amount to core

tag: dilution

tag: sample preservation

tag: strain or line

tag: ncbi taxonomy id

tag: individual


title: Nasal epithelial cells EB_0_5d_208_ACO1_THI08031_Rat230_2_THI 08031

description:
time point: 5d
dose: 0
EB_0_5d_208_ACO1_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

treatment_protocol: in vivo Treatment: Air Control, Treatment Repeat: 5, Units of Treatment Repeat: days per week, Treatment Duration: 5, Units of Duration: days, Treatment Time: 6, Units of Treatment Time: hours per day, Route of Exposure: Inhalation (whole-body), Dose or Concentration: 0, Units of Dose or Concentration: ppm

tag: units of amount

tag: rna integrity number

tag: bioanalyzer results

tag: developmental state

tag: sacrifice method

tag: sex


title: Nasal epithelial cells EB_10_13w_346_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 13w
dose: 10
EB_10_13w_346_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: array type

tag: ratio

tag: units of age

tag: age

tag: tissue

tag: tissue harvest methods


title: Nasal epithelial cells EB_10_4w_331_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 4w
dose: 10
EB_10_4w_331_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: amount to core

tag: dilution

tag: units of harvest time point

tag: time point

tag: agent

tag: dose


title: Nasal epithelial cells EB_0_5d_210_ACO1_THI08031_Rat230_2_THI 08031

description:
time point: 5d
dose: 0
EB_0_5d_210_ACO1_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: units of amount

tag: rna integrity number

tag: sample preservation

tag: strain or line

tag: ncbi taxonomy id

tag: individual


title: Nasal epithelial cells EB_10_13w_348_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 13w
dose: 10
EB_10_13w_348_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: array type

tag: ratio

tag: bioanalyzer results

tag: developmental state

tag: sacrifice method

tag: sex


title: Nasal epithelial cells EB_10_4w_333_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 4w
dose: 10
EB_10_4w_333_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: amount to core

tag: dilution

tag: units of age

tag: age

tag: tissue

tag: tissue harvest methods


title: Nasal epithelial cells EB_10_4w_336_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 4w
dose: 10
EB_10_4w_336_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: units of amount

tag: rna integrity number

tag: units of harvest time point

tag: time point

tag: agent

tag: dose


title: Nasal epithelial cells EB_10_13w_341_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 13w
dose: 10
EB_10_13w_341_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: array type

tag: ratio

tag: sample preservation

tag: strain or line

tag: ncbi taxonomy id

tag: individual


title: Nasal epithelial cells EB_0_5d_207_ACO1_THI08031_Rat230_2_THI 08031

description:
time point: 5d
dose: 0
EB_0_5d_207_ACO1_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: amount to core

tag: dilution

tag: bioanalyzer results

tag: developmental state

tag: sacrifice method

tag: sex


title: Nasal epithelial cells EB_0_4w_212_ACO2_THI08031_Rat230_2_THI 08031

description:
time point: 4w
dose: 0
EB_0_4w_212_ACO2_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: units of amount

tag: rna integrity number

tag: units of age

tag: age

tag: tissue

tag: tissue harvest methods


title: Nasal epithelial cells EB_10_4w_338_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 4w
dose: 10
EB_10_4w_338_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: array type

tag: ratio

tag: units of harvest time point

tag: time point

tag: agent

tag: dose


title: Nasal epithelial cells EB_0_4w_211_ACO2_THI08031_Rat230_2_THI 08031

description:
time point: 4w
dose: 0
EB_0_4w_211_ACO2_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: amount to core

tag: dilution

tag: sample preservation

tag: strain or line

tag: ncbi taxonomy id

tag: individual


title: Nasal epithelial cells EB_0_13w_225_ACO3_THI08031_Rat230_2_THI 08031

description:
time point: 13w
dose: 0
EB_0_13w_225_ACO3_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

treatment_protocol: in vivo Treatment: Air Control, Treatment Repeat: 5, Units of Treatment Repeat: days per week, Treatment Duration: 13, Units of Duration: weeks, Treatment Time: 6, Units of Treatment Time: hours per day, Route of Exposure: Inhalation (whole-body), Dose or Concentration: 0, Units of Dose or Concentration: ppm

tag: units of amount

tag: rna integrity number

tag: bioanalyzer results

tag: developmental state

tag: sacrifice method

tag: sex


title: Nasal epithelial cells EB_0_4w_217_ACO2_THI08031_Rat230_2_THI 08031

description:
time point: 4w
dose: 0
EB_0_4w_217_ACO2_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: array type

tag: units of age

tag: age

tag: tissue

tag: tissue harvest methods


title: Nasal epithelial cells EB_0_13w_229_ACO3_THI08031_Rat230_2_THI 08031

description:
time point: 13w
dose: 0
EB_0_13w_229_ACO3_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: amount to core

tag: ratio

tag: units of harvest time point

tag: time point

tag: agent

tag: dose


title: Nasal epithelial cells EB_0_13w_226_ACO3_THI08031_Rat230_2_THI 08031

description:
time point: 13w
dose: 0
EB_0_13w_226_ACO3_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: units of amount

tag: dilution

tag: sample preservation

tag: strain or line

tag: ncbi taxonomy id

tag: individual


title: Nasal epithelial cells EB_0_4w_213_ACO2_THI08031_Rat230_2_THI 08031

description:
time point: 4w
dose: 0
EB_0_4w_213_ACO2_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: array type

tag: rna integrity number

tag: bioanalyzer results

tag: developmental state

tag: sacrifice method

tag: sex


title: Nasal epithelial cells EB_07_5d_240_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 5d
dose: 0.7
EB_07_5d_240_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

treatment_protocol: in vivo Treatment: Formaldehyde, Treatment Repeat: 5, Units of Treatment Repeat: days per week, Treatment Duration: 5, Units of Duration: days, Treatment Time: 6, Units of Treatment Time: hours per day, Route of Exposure: Inhalation (whole-body), Dose or Concentration: 0.7, Units of Dose or Concentration: ppm

tag: amount to core

tag: ratio

tag: units of age

tag: age

tag: tissue

tag: tissue harvest methods


title: Nasal epithelial cells EB_07_5d_234_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 5d
dose: 0.7
EB_07_5d_234_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: units of amount

tag: dilution

tag: units of harvest time point

tag: time point

tag: agent

tag: dose


title: Nasal epithelial cells EB_07_4w_245_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 4w
dose: 0.7
EB_07_4w_245_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: array type

tag: rna integrity number

tag: sample preservation

tag: strain or line

tag: ncbi taxonomy id

tag: individual


title: Nasal epithelial cells EB_07_5d_235_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 5d
dose: 0.7
EB_07_5d_235_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: amount to core

tag: ratio

tag: bioanalyzer results

tag: developmental state

tag: sacrifice method

tag: sex


title: Nasal epithelial cells EB_07_4w_242_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 4w
dose: 0.7
EB_07_4w_242_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: units of amount

tag: dilution

tag: units of age

tag: age

tag: tissue

tag: tissue harvest methods


title: Nasal epithelial cells EB_0_5d_204_ACO1_THI08031_Rat230_2_THI 08031

description:
time point: 5d
dose: 0
EB_0_5d_204_ACO1_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: array type

tag: rna integrity number

tag: units of harvest time point

tag: time point

tag: agent

tag: dose


title: Nasal epithelial cells EB_0_13w_230_ACO3_THI08031_Rat230_2_THI 08031

description:
time point: 13w
dose: 0
EB_0_13w_230_ACO3_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: amount to core

tag: ratio

tag: sample preservation

tag: strain or line

tag: ncbi taxonomy id

tag: individual


title: Nasal epithelial cells EB_07_13w_259_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 13w
dose: 0.7
EB_07_13w_259_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

treatment_protocol: in vivo Treatment: Formaldehyde, Treatment Repeat: 5, Units of Treatment Repeat: days per week, Treatment Duration: 13, Units of Duration: weeks, Treatment Time: 6, Units of Treatment Time: hours per day, Route of Exposure: Inhalation (whole-body), Dose or Concentration: 0.7, Units of Dose or Concentration: ppm

tag: units of amount

tag: dilution

tag: bioanalyzer results

tag: developmental state

tag: sacrifice method

tag: sex


title: Nasal epithelial cells EB_07_13w_260_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 13w
dose: 0.7
EB_07_13w_260_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: array type

tag: rna integrity number

tag: units of age

tag: age

tag: tissue

tag: tissue harvest methods


title: Nasal epithelial cells EB_07_13w_257_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 13w
dose: 0.7
EB_07_13w_257_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: amount to core

tag: ratio

tag: units of harvest time point

tag: time point

tag: agent

tag: dose


title: Nasal epithelial cells EB_07_5d_237_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 5d
dose: 0.7
EB_07_5d_237_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: units of amount

tag: dilution

tag: sample preservation

tag: strain or line

tag: ncbi taxonomy id

tag: individual


title: Nasal epithelial cells EB_07_13w_258_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 13w
dose: 0.7
EB_07_13w_258_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: array type

tag: rna integrity number

tag: bioanalyzer results

tag: developmental state

tag: sacrifice method

tag: sex


title: Nasal epithelial cells EB_07_4w_243_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 4w
dose: 0.7
EB_07_4w_243_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: amount to core

tag: ratio

tag: units of age

tag: age

tag: tissue

tag: tissue harvest methods


title: H37Rv_1hour reaeration_rep4

city: Seattle

first: Ashley

middle: M

last: Sherrid

state: WA

address: 307 Westlake Ave N

institute: Seattle Biomedical Research Institute

hyb_protocol: Slides prehybed for >1 hour in prehyb buffer (5X SSC, 0.1% SDS, 1% BSA), washed 2X for 1 minute in water, 15 seconds in isopropanol, dried by centrifugation. Probes added to slides, sealed in incubation chambers and submerged in 42 degree incubator overnight. Arrays washed in increasingly stringent washes: 5 minutes at 42 degrees low stringency wash (0.2%SDS, 1XSSC), 10 minutes lower stringency wash (0.2%SDS, 0.1XSSC), 4 times 1 minute in 0.1XSSC, 10 seconds in 0.01XSSC. Slides dried by centrifugation.

scan_protocol: Arrays scanned in with a GenePix4000B using GenePix 6 scanning software. Gain adjusted during preview scan to roughly equivalence between channels with a few saturated spots.

data_processing:
Data analysis conducted with Acuity 4 software package. Spots flagged as not found were excised from the dataset. Remaining features were lowess normalized seperately for each print tip.
Mean of all not-flagged features for each substance lowess normalized log2 ratio (hypoxic sample/log phase sample) is presented in the data table below.

organism: Mycobacterium tuberculosis

source_name: 7 day hypoxia RNA

label_protocol: Standard amino-allyl labelling protocol from TIGR. cDNA made with Superscript III using aminoallyl dUTP. cDNA purified using Qiaquick column from Qiagen. Tags conjugated to reactive Cy dyes in the presence of sodium carbonate. Excess Cy dye removed with a Qiaquick column from Qiagen. Probes resuspended in hyb buffer (50% formamide, 5X SSC, and  0.1% SDS) and kept at room temp until added to array slides.

growth_protocol: Grown to mid log phase (OD600 0.1-0.3) in roller bottles with a 5:1 headspace ratio in Middlebrook 7H9 media + ADC. Diluted to an OD of 0.1 and cultured for seven days with constant agitation and continuous exposure to 0.2% oxygen (balance nitrogen). Transferred to roller bottles with 5:1 headspace ratio.

extract_protocol: Pellets were bead beat in the presence of trizol. Aqueus phase isolated using phase lock gel. RNA precipitated in 50% isopropanol 50% high salt solution ((0.8M Na Citrate, 1.2M NaCl). RNA purified using Rneasy columns from Qiagen.

treatment_protocol: Cultures adapted to 0.2% oxygen (balance nitrogen) for seven days, were transferred to roller bottles with normal atmospheric oxygen and incubated rolling at 37˚C. Samples were collected by pipet and centrifuged at 3500rcf for 10 minute,s then supernatant was removed and pellet was flash frozen on dry ice.

tag: strain

tag: culture condition

value: 7 days with continuous 0.2% oxygen

source_name: 1 hours reaeration RNA

value: 1 hours with atmospheric air


title: H37Rv_4hour reaeration_rep8

source_name: 4 hours reaeration RNA

value: 4 hours with atmospheric air


title: H37Rv_4hour reaeration_rep7


title: H37Rv_4hour reaeration_rep6


title: H37Rv_1hour reaeration_rep2


title: H37Rv_1hour reaeration_rep3


title: Nasal epithelial cells EB_07_4w_250_HCHO_THI08031_Rat230_2_THI 08031

description:
time point: 4w
dose: 0.7
EB_07_4w_250_HCHO_THI08031_Rat230_2_THI 08031: Eight week old male F344/CrlBR rats were exposed to formaldehyde through whole body inhalation. Whole-body exposures were performed at doses of 0, 0.7, 2, 6, 10, and 15 ppm (6 hours per day, 5 days per week).  Inhalation animals were sacrified at 1, 4, and 13 weeks following initiation of exposure.  Following sacrifice, tissue from the Level II region of the nose was dissected and digested with a mixture of proteases to remove the epithelial cells.  The epithelial cells acquired from this section of the nose consisted primarily of transitional epithelium with some respiratory epithelium.  Gene expression microarray analysis was performed on the epithelial cells.

tag: units of amount

tag: dilution

tag: sample preservation

tag: strain or line

tag: units of harvest time point

tag: time point

tag: agent

tag: dose


title: H37Rv_24hour reaeration_rep6

treatment_protocol: Cultures adapted to 0.2% oxygen (balance nitrogen) for seven days, were transferred to roller bottles with normal atmospheric oxygen and incubated rolling at 37˚C. Samples were collected by pipet at various timepoints and centrifuged at 3500rcf for 10 minutes, then supernatant was removed and pellet was flash frozen on dry ice.

source_name: 24 hours reaeration RNA

value: 24 hours with atmospheric air


title: H37Rv_4hour reaeration_rep8


title: H37Rv_4hour reaeration_rep7


title: H37Rv_1hour reaeration_rep1


title: H37Rv_4hour reaeration_rep5


title: H37Rv_4hour reaeration_rep6


title: H37Rv_24hour reaeration_rep3


title: H37Rv_24hour reaeration_rep4


title: H37Rv_4hour reaeration_rep5


title: H37Rv_1hour reaeration_rep7


title: H37Rv_24hour reaeration_rep2


title: H37Rv_24hour reaeration_rep1


title: H37Rv_1hour reaeration_rep8


title: H37Rv_1hour reaeration_rep6


title: H37Rv_24hour reaeration_rep5


title: H37Rv_12hour reaeration_rep3

source_name: 12 hours reaeration RNA

value: 12 hours with atmospheric air


title: H37Rv_12hour reaeration_rep4


title: GenAu MB BCELL v4.0 H3K4me2 wt

type: genomic

city: Vienna

first: Meinrad

last: Busslinger

address: Dr. Bohrgasse 7

country: Austria

institute: Instutute of Molecular Pathology

description: Chip-chip wt pro-B cells H3K4me2

hyb_protocol: The labeled ChIP DNA was precipitated with 0.1 volume 5M NaCl and 1 volume isopropanol, and hybridized in 45 ul of buffer containing 20% formamide, 1.2 M betaine, 0.1 ug/ul herring sperm DNA and 10 ug of human COT1 DNA (Invitrogen). Arrays were hybridized in Maui hybridization stations for 16-18 h at 42C, and then washed in 42C 0.2% SDS/0.2x SSC, room temperature 0.2x SSC, and 0.05x SSC. Hybridization buffers and washes were completed using manufacturer's protocols (http://www.nimblegen.com/products/lit/lit.html)

scan_protocol: Arrays were scanned on an Axon 4000B scanner per manufacturer's protocol (http://www.nimblegen.com/products/lit/lit.html).

data_processing:
Performed using custom R script.
log2(Signal Cy5/Signal Cy3) - tukey.biweight(Signal Cy5/Signal Cy3)

molecule: genomic DNA

organism: Mus musculus

source_name: H3K4me2 ChIP DNA from wt pro-B cells

label_protocol: 1 µg ChIP DNA was directly labeled by Klenow (New England Biolabs) random priming with Cy5 nonamers (ChIP DNA isolated from normal rabbit IgG-immunoprecipitated cells) per manufacturer's protocol (http://www.nimblegen.com/products/lit/lit.html).

growth_protocol: pro-B cells were cultured for 5 days in the presence of IL-7 on OP9 feeder cells.

extract_protocol: 5x10E6 cells were fixed with 1% formaldehyde in culture medium for 10 min at room temperature followed by quenching with 0.125 M glycine for 5 min. The cells were washed twice with ice-cold PBS and lysed in 1 ml of lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris pH8.0) for at least 30 min on ice. The crosslinked chromatin was sheared to an average size of 500 bp by ten 30-second pulses using a BiorupterTM sonicator (Diagenode). The lysate was then centrifuged to remove cell debris, and the chromatin was quantified in a UV spectrophotometer. The chromatin (400 µg) was diluted 10-fold in dilution buffer (0.01% SDS, 1.1% Triton X-100, 1.2 mM EDTA 16.7 mM Tris pH8.10, 167 mM NaCl) and pre-cleared with 25 µl protein A sepharose beads (pre-absorbed with salmon sperm DNA and BSA) for 2 h at 4C. The chromatin supernatant was then incubated overnight with 5 µg of purified antibody at 4C. After incubation with protein A sepharose beads (50 µl) for 2h at 4C, the immune complexes were collected by centrifugation and washed with the following buffers each for 10 min at 4C; RIPA buffer (150 mM NaCl, 50 mM Tris pH8.0, 0.1% SDS), high-salt buffer(500 mM NaCl, 50 mM Tris pH8.0, 0.1% SDS, 1% NP40) LiCl buffer (250 mM LiCl, 50 mM Tris pH8.0, 0.5% Na deoxycholate, 1% NP40) and 2x TE (20 mM Tris pH8.0, 2 mM EDTA). The protein-DNA complexes were eluted from the beads in 450 µl elution buffer (1% SDS, 100 mM NaHCO3) at 55C for 2 h followed by the addition of proteinaseK to 500 µg/ml and overnight incubaton at 65C. Genomic DNA was isolated from the precipitated material as well as from the sheared chromatin input (1/100 of the material used for ChIP) by phenol extraction and ethanol precipitation.

treatment_protocol: pro-B cells were MACs sorted from the bone marrow of 3-5 week old mice.

tag: genotype

value: wt

value: 5 days in culture

value: pro-B cells

tag: antibody

tag: type

value: ChIP

source_name: Input DNA from wt pro-B cells

growth_protocol: see channel 1

extract_protocol: see channel 1

treatment_protocol: see channel 1

value: Input


title: H37Rv_12hour reaeration_rep2


title: GenAu MB BCELL v4.0 H3Kme3 wt

description: Chip-chip wt pro-B cells H3Kme3

source_name: H3Kme3 ChIP DNA from wt pro-B cells


title: GenAu MB BCELL v4.0 H3Kme3 Rag2-/-

description: Chip-chip Rag2-/- pro-B cells H3Kme3

source_name: H3Kme3 ChIP DNA from Rag2-/- pro-B cells

source_name: Input DNA from Rag2-/- pro-B cells


title: H37Rv_12hour reaeration_rep6


title: H37Rv_12hour reaeration_rep5


title: GenAu MB BCELL v4.0 H3K9ac Rag2-/-

description: Chip-chip Rag2-/- pro-B cells H3K9ac

source_name: H3K9ac ChIP DNA from Rag2-/- pro-B cells


title: H37Rv_6hour reaeration_rep5

source_name: 6 hours reaeration RNA

value: 6 hours with atmospheric air


title: GenAu MB BCELL v4.0 H3K4me2 Rag2-/-

description: Chip-chip Rag2-/- pro-B cells H3K4me2

source_name: H3K4me2 ChIP DNA from Rag2-/- pro-B cells


title: GenAu MB BCELL v4.0 H3K9ac wt

description: Chip-chip wt pro-B cells H3K9ac

source_name: H3K9ac ChIP DNA from wt pro-B cells


title: H37Rv_1hour reaeration_rep5


title: H37Rv_12hour reaeration_rep1


title: H37Rv_6hour reaeration_rep3


title: H37Rv_6hour reaeration_rep4


title: H37Rv_6hour reaeration_rep1


title: H37Rv_6hour reaeration_rep2


title: ME7 + LPS, A37

city: London

first: Katie

middle: Sarah

last: Lunnon

address: De crespigny Park

department: Old Age Psychiatry

country: United Kingdom

institute: Institute of Psychiatry

description: Gene expression data from mouse injected with ME7 prion homogenate i.c. 18 weeks subsequently was administered 500ug/kg LPS i.p. And sacrificed 6 hours later.

hyb_protocol: Standard Affymetrix protocol

scan_protocol: Standard Affymetrix protocol

data_processing: Standard Affymetrix protocol, Algorithm: ExpressionStat 5.0

source_name: ME7 + LPS hippocampal RNA

label_protocol: Standard Affymetrix protocol

growth_protocol: Mice were innocluated i.c. With 1ul of Normal Brain Homogenate (NBH) or 1ul of brain homogenate from a mouse with terminal stages of ME7 prion disease

extract_protocol: Mice were rapidly perfused with heparinised-saline and the left hippocampus was removed and stored in RNA later. Total RNA was extracted using the Qiagen Rneasy mini kit and bioanalysed to assess the quality. Total RNA was stored at -80C until required for microarrays.

treatment_protocol: 18 weeks post-innoculation mice were administered 500ug/kg LPS or an equivalent volume of saline. Mice were sacrificed 6 hours subsequently.

value: Hippocampal tissue punch

tag: innoculation

value: ME7 prion disease

value: 500ug/kg LPS


title: ME7 + LPS, A36


title: ME7 + LPS, A35


title: ME7 + saline, A34

description: Gene expression data from mouse innoculated with ME7 prion homogenate i.c. 18 weeks subsequently was administered saline i.p. And sacrificed 6 hours later.

source_name: ME7 + saline hippocampal RNA

value: saline


title: Risk haplotype_MGA1014

city: Barcelona

first: Unitat

middle: Estadistica

last: Bioinformatica

address: Passeig Vall d'Hebron, 119-129

department: Statistics and Bioinformatics Unit

country: Spain

institute: Vall d'Hebron Institut de Recerca

hyb_protocol: Samples were hybridized using Affymetrix hybridization kit materials.

scan_protocol: Affymetrix GeneChIP Scanner 3000 7G.

data_processing: Chips were processed on an Affymetrix GeneChip Fluidics Station 450. Background adjustment by Robust Multi-Array (RMA) algorithm was applied to probes intensities using R-2.8 with Bioconductor 2.3 packages. Raw probes gene level expression were summarized from the corrected intensities and then quantile normalized.

organism: Homo sapiens

source_name: Peripheral blood mononuclear cells, risk haplotype

label_protocol: Samples were chemically fragmented and biotinylated using the IVT Labelling Kit (Affymetrix).

extract_protocol: Total RNA was extracted from fresh peripheral blood mononuclear cells using an RNeasy kit (Quiagen, Santa Clarita, USA).

tag: gender

value: male

value: blood

tag: cell type

value: peripheral blood mononuclear cells

tag: prf1 haplotype

value: AGG/GAA

tag: haplotype risk

value: risk

tag: disease

value: primary-progressive multiple sclerosis (PPMS)


title: Risk haplotype_PFB1530

tag: tissue


title: Risk haplotype_UUS1554


title: Risk haplotype_AGS1013


title: ME7 + saline, A33

tag: strain


title: Risk haplotype_RAU1550


title: Protective haplotype_EMA1473

source_name: Peripheral blood mononuclear cells, protective haplotype

value: AGA/AGA

value: protective


title: liver cell

city: Tsukuba

first: Arata

last: Honda

country: Japan

institute: RIKEN BRC

description: Gene expression in rabbit liver cells

hyb_protocol: 1.65 ug of Cy3-labelled cRNA (specific activity >10.0 pmol Cy3/ug cRNA) was fragmented at 60°C for 29 minutes in a reaction volume of 110 ul containing 25 x Agilent fragmentation buffer and 10 x Agilent blocking agent following the manufacturers instructions. On completion of the fragmentation reaction, 55 ul of 2x Agilent hybridization buffer was added to the fragmentation mixture and hybridized to the rabbit 44k oligo array, G2519F (Agilent Technologies) for 17 hours at 65°C in a rotating Agilent hybridization oven.  After hybridization, microarrays were washed 1 minute at room temperature with GE Wash Buffer 1 (Agilent) and 1 minute with 37°C GE Wash buffer 2 (Agilent), then dried immediately by brief centrifugation.

scan_protocol: Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2565CA) using one color scan setting for 4x44k array slides (Scan Area 61x21.6 mm, Scan resolution 5 um, Dye channel is set to Green and Green PMT is set to 100%).

data_processing: The scanned images were analyzed with Feature Extraction Software GX11 (Agilent) using default parameters (protocol GE1-105_Dec08 and Grid: 020908_D_F_20100202) to obtain background subtracted and spatially detrended Processed Signal intensities.  Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.

organism: Oryctolagus cuniculus

source_name: rabbit liver cell

label_protocol: Cyanine-3 (Cy3) labeled cRNA was prepared from 0.5 ug total RNA was labeled with Cy3 dye with Quick Amp Labeling kit (Agilent Technologies) according to the manufacturer's instructions, followed by RNeasy column purification (QIAGEN, Valencia, CA).  Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.

growth_protocol: Cells were cultured using stem cell medium (rdES cells and iPS cells) or somatic cell medium (liver cells and stomach cells) at 37 ºC in a humidified incubator with 5% CO2.

extract_protocol: Total RNA was isolated using ISOGEN (Nippon Gene, Toyama, Japan) from cells cultured under different conditions. After DNase treatment to prevent genomic DNA contamination. RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).

value: Dutch rabbit

value: liver cells


title: Risk haplotype_RPS1011


title: Protective haplotype_MMC-998


title: Protective haplotype_UOM982


title: ME7 + saline, A31


title: iPS-L1 P23

description: Gene expression in rabbit iPS cells at P23 which was generated from rabbit liver cells

source_name: rabbit iPS cell from liver P23

value: iPS cells at P23 which was generated from liver cells


title: HeLa SCY1-like 1 knock down 3

city: Erlangen

first: Ingo

last: Hermann

address: Schwabachanlage 10

country: Germany

institute: Humangenetisches Institut

hyb_protocol: standard Affymetrix protocol

scan_protocol: standard Affymetrix protocol

data_processing: Data analysed with PARTEK Genomic Suite software with standard parameters

source_name: HeLa cell line treated with esiRNA

extract_protocol: Qiagen RNAeasy kit

treatment_protocol:
transfection of esiRNA with Lipofectamine 2000 (Invitrogen)
RNA isolation 24 h after transfection

tag: treatment

value: SCY1-like 1 esiRNA

tag: cell line

value: HeLa


title: iPS-S1 P22

description: Gene expression in rabbit iPS cells at P22 which was generated from rabbit stomach cells

source_name: rabbit iPS cell from stomach P22

value: iPS cells at P22 which was generated from stomach cells


title: HeLa SCY1-like 1 knock down 2


title: stomach cell

description: Gene expression in rabbit stomach cells

source_name: rabbit stomach cell

value: stomach cells


title: iPS-L1 P7

description: Gene expression in rabbit iPS cells at P7 which was generated from rabbit liver cells

source_name: rabbit iPS cell from liver P7

value: iPS cells at P7 which was generated from liver cells


title: NBH + LPS, A42

description: Gene expression data from mouse injected with Normal Brain homogenate i.c. 18 weeks subsequently was administered 500ug/kg LPS i.p. And sacrificed 6 hours later.

source_name: NBH + LPS hippocampal RNA

value: Hippocampal tissue punch

value: Normal Brain Homogenate (NBH)

value: 500ug/kg LPS


title: rdES2-1

description: Gene expression in rabbit embryonic stem cell

source_name: rabbit embryonic ste cell

value: embryonic stem cell


title: HeLa SCY1-like 1 knock down 1


title: NBH + LPS, A41


title: iPS-S1 P6

description: Gene expression in rabbit iPS cells at P6 which was generated from rabbit stomach cells

source_name: rabbit iPS cell from stomach P6

value: iPS cells at P6 which was generated from stomach cells


title: rdES4

source_name: rabbit embryonic stem cell


title: GenAu MB BCELL v3.0 H3K9ac Rag2-/-

description: Chip-chip Rag2-/- pro-B cells H3K9ac

source_name: H3K9ac ChIP DNA from Rag2-/- pro-B cells

tag: age

value: 5 days in culture

value: pro-B cells

value: ChIP

source_name: Input DNA from Rag2-/- pro-B cells

value: Input


title: HeLa control knock down 3

source_name: HeLa cell line

value: control esiRNA


title: NBH + saline, A38

description: Gene expression data from mouse injected with Normal Brain homogenate i.c. 18 weeks subsequently was administered saline i.p. And sacrificed 6 hours later.

source_name: NBH + saline hippocampal RNA

value: saline


title: HeLa control knock down 1


title: NBH + saline, A40


title: HMEC, Canicola vs. uninfected, 3 hr, rep 3

status: Public on Jul 27 2010

city: Milwaukee

first: Jenifer

last: Coburn

state: WI

address: 8701 Watertown Plank Rd.

department: ID/CBID

institute: Medical College of Wisconsin

description: comparison of HMEC cells infected with L. interrogans Canicola for 3 hr vs. uninfected cells

hyb_protocol: The two differently labeled cDNAs were mixed and hybridized using Pronto Microarray Hybridization Kit in a hybridization chamber (Corning, Corning, NY), with the same array slide for 38 to 42 hr according to manufacturer’s instruction.   After a series of washes using the buffers provided in the kit, slides were spun dry.

scan_protocol: The slides were scanned under two laser channels in a Scanarray 4000 scanner (Packard Bioscience, Meriden, CT).

data_processing:
Images were overlaid and analyzed using Imagene (BioDiscovery, El Segundo, CA).   Raw gene expression was imported from Imagene to GeneSifter (GeneSifter.Net, VizX Labs, Seattle, WA) for analysis. Data from 3 biological replicate experiments were normalized using Lowess normalization and by the median of the raw intensities for all spots in each sample for each array.
Normalized data are unavailable (not provided by the submitter).

source_name: uninfected 3 hr

label_protocol: 5 to 20 mg of total RNA from uninfected control and infected samples was used to generate cDNA labeled with aminoallyl (aa)-dUTP through a reverse transcription reaction using anchored oligo(dT) primers.  The purified aa-dUTP-labeled cDNAs were coupled in 10 µl 0.1 M NaHCO3 with either Cy3 or Cy5 NHS-ester dye. Cy-dye labeled cDNA was purified using a Cyscribe GFX column (Amersham Biosciences, Piscataway, NJ).

extract_protocol: Cells were washed with phosphate buffered saline (PBS) and harvested for RNA isolation.  The RNA was purified using RNeasy kit (Qiagen, Valencia, CA) with DNase digestion according to manufacturer’s manual. The quality of RNA was checked using a Bioanalyzer (Agilent, Santa Clara, CA).

value: microvascular endothelial cells

value: HMEC

tag: infection status

value: uninfected 3 hr

tag: rep number

value: replicate experiment 3

source_name: infected with L. interrogans 3 hr

value: infected with L. interrogans 3 hr


title: HMEC, Canicola vs. uninfected, 1 hr, rep 3

description: comparison of HMEC cells infected with L. interrogans Canicola for 1 hr vs. uninfected cells

source_name: uninfected 1 hr

value: uninfected 1 hr

source_name: infected with L. interrogans 1 hr

value: infected with L. interrogans 1 hr


title: HMEC, Patoc vs. uninfected, 3 hr, rep 3

description: comparison of HMEC cells infected with L. biflexa Patoc for 3 hr vs. uninfected cells

source_name: infected with L. biflexa 3 hr

value: infected with L. biflexa 3 hr


title: DE, A3

city: Heidelberg

first: Lukas

last: Chavez

address: Im Neuenheimer Feld 280

institute: German Cancer Research Center (DKFZ)

description: replicate 2

hyb_protocol: Standard Illumina hybridization protocol

scan_protocol: Standard Illumina scanning protocol

data_processing: Raw (non-normalized) data were exported using Illumina's BeadStudio software version 3.0.14.0 as a 'Sample Gene Profile' and imported the R environment version 2.9.0. Subsequently, quantile normalization was performed using the beadarray package version 1.12.0. Finally, differential expression was calculated using limma version 2.18.1 without any other filtering or data processing steps.

label_protocol: Illumina Gene Expression protocols contain a first- and second-strand reverse transcription step, followed by a single in vitro transcription (IVT) amplification that incorporates biotin labeled nucleotides. Subsequent steps include array hybridization, washing, blocking, and streptavadin-Cy3 staining. Fluorescence emission by Cy3 is quantitatively detected for downstream analysis.

extract_protocol: The RNA was isolated using RNeasy Mini Kit (Quiagen), and DNase digestion (RNase-free DNase Set, Quiagen) has been applied on the columns during isolation.

value: hESC line H1

tag: cell passage

value: Passage 53

value: hESCs

tag: activation

value: Activin-A

tag: cell subtype

value: definitive endoderm (DE)


title: HMEC, Patoc vs. uninfected, 1 hr, rep 3

description: comparison of HMEC cells infected with L. biflexa Patoc for 1 hr vs. uninfected cells

source_name: infected with L. biflexa 1 hr

value: infected with L. biflexa 1 hr


title: HMEC, Canicola vs Patoc, 3 hr, rep 3

description: comparison of HMEC cells infected with L. interrogans Canicola vs. cells infected with L. biflexa Patoc for 3 hr


title: DE, A4

description: replicate 3


title: HMEC, Canicola vs Patoc, 1 hr, rep 3

description: comparison of HMEC cells infected with L. interrogans Canicola vs. cells infected with L. biflexa Patoc for 1 hr


title: NBH + saline, A39

value: Hippocampal tissue punch

value: Normal Brain Homogenate (NBH)


title: HeLa control knock down 2

value: control esiRNA

value: HeLa


title: GenAu MB BCELL v3.0 H3K4me2 Rag2-/-

description: Chip-chip Rag2-/- pro-B cells H3K4me2

source_name: H3K4me2 ChIP DNA from Rag2-/- pro-B cells

tag: genotype

value: 5 days in culture

value: pro-B cells

tag: antibody

tag: type

value: ChIP

value: Input


title: GenAu MB BCELL v3.0 H3Kme3 Rag2-/-

description: Chip-chip Rag2-/- pro-B cells H3Kme3

source_name: H3Kme3 ChIP DNA from Rag2-/- pro-B cells


title: DE, A2

description: replicate 1

tag: cell type


title: HMEC, Canicola vs. uninfected, 3 hr, rep 2

value: microvascular endothelial cells

value: HMEC

value: uninfected 3 hr

value: replicate experiment 2

value: infected with L. interrogans 3 hr


title: HMEC, Patoc vs. uninfected, 1 hr, rep 2

value: uninfected 1 hr


title: hESCs, C3

description: replicate 3

value: hESC line H1

value: Passage 53

value: hESCs

value: control


title: HMEC, Canicola vs. uninfected, 1 hr, rep 2

value: infected with L. interrogans 1 hr


title: HMEC, Canicola vs Patoc, 3 hr, rep 2

value: infected with L. biflexa 3 hr


title: hESCs, C2

description: replicate 2


title: HMEC, Canicola vs Patoc, 1 hr, rep 2

value: infected with L. biflexa 1 hr


title: HMEC, Patoc vs. uninfected, 1 hr, rep 1

value: replicate experiment 1


title: HMEC, Canicola vs. uninfected, 3 hr, rep 1


title: HMEC, Canicola vs. uninfected, 1 hr, rep 1


title: Twist ChIP after30 min. Notch activation replicate 1

city: Cambridge

first: Sarah

last: Bray

address: Downing Street

department: PDN

institute: University  of Cambridge

hyb_protocol: Hybridisation has been done with HX1 mixer following Nimblegen's procedures

scan_protocol: Scanned image and extraction of the data have been performed using NimbleScan software v2.6

data_processing: The vsn package within Bioconductor (vsn2 function) was used to normalise across all channels (ChIP and Input) within the project. The ratios in our files can be treated as log2 vsn normalised as we have applied a function to convert the resulting vsn glog ratios into log2.

organism: Drosophila melanogaster

source_name: Total Input

label_protocol: Labeling has been done using BioPrime Kit (Invitrogen) based on a Kleenow enzymatic reaction following manufacturer's protocol

growth_protocol: DmD8 cells were grown at 25C in Schneider's Drosophila Medium (Invitrogen) supplemented with 10% fetal calf serum, 100ug/ml streptamycin and 100 units/ml penicilin and with 10ug/ml insulin (Sigma). Cultures were split and replated in fresh medium approximately 12-18 hours prior to activation.

extract_protocol: DmD8 cells have been fixed with 1.5% fresh formaldehyde (SIGMA) after 30 min of 2mM EDTA treatment and processed as described in Krejci et al. 2009

treatment_protocol: Notch activation was achieved by exposing cells to 2mM EDTA in PBS for 30 minutes.

value: DmD8 suscle progenitor cell line

tag: chip antibody

source_name: Immunoprecipitated DNA

value: Twist (S, Rorth; PubMedID:2688897)


title: NBH + LPS, A43

source_name: NBH + LPS hippocampal RNA

value: Hippocampal tissue punch

tag: innoculation

value: Normal Brain Homogenate (NBH)

value: 500ug/kg LPS


title: HMEC, Canicola vs Patoc, 3 hr, rep 1

value: microvascular endothelial cells

value: HMEC


title: Protective haplotype_CDP1842

source_name: Peripheral blood mononuclear cells, protective haplotype

tag: gender

value: male

value: blood

value: peripheral blood mononuclear cells

tag: prf1 haplotype

value: AGA/AGA

tag: haplotype risk

value: protective

tag: disease

value: primary-progressive multiple sclerosis (PPMS)


title: HMEC, Canicola vs Patoc, 1 hr, rep 1

tag: cell line

value: infected with L. interrogans 1 hr


title: hESCs, C1

description: replicate 1

value: hESC line H1

value: Passage 53

value: hESCs

value: control


title: Twist ChIP after30 min. Notch activation replicate 2

value: DmD8 suscle progenitor cell line

value: Twist (S, Rorth; PubMedID:2688897)


title: HMEC, Patoc vs. uninfected, 3 hr, rep 2

value: uninfected 3 hr

value: replicate experiment 2

value: infected with L. biflexa 3 hr


title: Twist ChIP after30 min. Notch activation replicate 3


title: HMEC, Patoc vs. uninfected, 3 hr, rep 1

value: microvascular endothelial cells

value: HMEC

value: replicate experiment 1


title: Eahy3.Can.1H.Cy5

description: Eahy, Canicola vs. uninfected, 1 hr, Cy5

data_processing:
Images were overlaid and analyzed using Imagene (BioDiscovery, El Segundo, CA). Raw gene expression was imported from Imagene to GeneSifter (GeneSifter.Net, VizX Labs, Seattle, WA) for analysis.
Data from 3 biological replicate experiments were normalized using Lowess normalization and by the median of the raw intensities for all spots in each sample for each array.
Each channel from the dual-channel hybridized array was analyzed independantly. The same raw data file is therefore associated with two Samples.

value: macrovascular cell line

tag: replicate

value: replicate experiment 3


title: Eahy3.Can.3H.Cy5

description: Eahy, Canicola vs. uninfected, 3 hr, Cy5


title: Eahy3.uninf.1H.Cy5

description: Eahy, Patoc vs. uninfected, 1 hr, Cy5


title: Eahy3.Patoc.3H.Cy3

description: Eahy, Patoc vs. uninfected, 3 hr, Cy3


title: Eahy3.uninf.3H.Cy3

description: Eahy, Canicola vs. uninfected, 3 hr, Cy3


title: Eahy3.Can.3H.Cy3

description: Eahy, Canicola vs Patoc, 3 hr, Cy3


title: Eahy3.Pat.1H.Cy5

description: Eahy, Canicola vs Patoc, 1 hr, Cy5


title: Eahy2.Patoc.3H.Cy3

description: Eahy, Patoc vs. uninfected, 3 hr, Cy3


title: Eahy3.uninf.1H.Cy3

description: Eahy, Canicola vs. uninfected, 1 hr, Cy3


title: Eahy2.uninf.1H.Cy5

description: Eahy, Patoc vs. uninfected, 1 hr, Cy5


title: Eahy2.uninf.3H.Cy3


title: Eahy2.Can3H.Cy5


title: Eahy3.Can.1H.Cy3

description: Eahy, Canicola vs Patoc, 1 hr, Cy3


title: Eahy3.Pat.3H.Cy5

description: Eahy, Canicola vs Patoc, 3 hr, Cy5


title: Eahy2.uninf.3H.Cy5

description: Eahy, Patoc vs. uninfected, 3 hr, Cy5


title: Eahy3.Patoc.1H.Cy3

description: Eahy, Patoc vs. uninfected, 1 hr, Cy3


title: Eahy2.Pat.3H.Cy5

description: Eahy, Canicola vs Patoc, 3 hr, Cy5


title: Eahy2.Can.1H.Cy5


title: Eahy2.Patoc.1H.Cy3

description: Eahy, Patoc vs. uninfected, 1 hr, Cy3


title: Eahy3.uninf3H.Cy5

description: Eahy, Patoc vs. uninfected, 3 hr, Cy5


title: Eahy2.Can.3H.Cy3

description: Eahy, Canicola vs Patoc, 3 hr, Cy3