title: SUM159

hyb_protocol: RNA was labeled and hybridized to Illumina human Ref-8 v2 Expression BeadChIP microarrays according to the manufacturer’s instructions

scan_protocol: Illumina human Ref-8 v2 Expression BeadChIP microarrays were scanned according to the manufacturer’s instructions.

source_name: Human breast cancer cell line

value: Human breast cancer cell line


title: MCF7

status: Public on Jan 15 2011

type: RNA

city: Seattle

first: Bin

last: Zhang

state: WA

address: 1100 Fairview Ave North

department: Network Biology

country: USA

institute: Sage Bionetworks

description: replicate 2

data_processing:
Raw data were background subtracted and quantile normalized using Illumina Bead Studio software version 3
Unnormalized data are signal intensity with background subtracted; normalized data are signal intensity with background subtracted and quantile normalized using Illumina Bead Studio software

label: biotin

molecule: total RNA

organism: Homo sapiens

source_name: Human Breast cancer cell line

label_protocol: RNA was labeled and hybridized to Illumina human Ref-8 v2 Expression BeadChIP microarrays according to the manufacturer’s instructions

growth_protocol: cells were cultured according to standard procotols.

extract_protocol: Total RNA was isolated using Trizol and RNA purification kit.

tag: cell line

tag: cell type


title: 4.9 Replicate 2

city: Wuerzburg

first: Jaroslaw

last: Zdziarski

address: Josef-Schneider Str. 2/Bau D15

department: Institut fuer Molekulare Infektionsbiologie

country: Germany

institute: Universitaet Wuerzburg

description: Biological replicate 2 of 3. Culture of re-isolate 4.9 in pooled human urine harvested at 0.15 OD

hyb_protocol: Labeled and purified probes (160 pmol each) were applied on pre-hybridized arrays and incubated for 14-16 hours. After hybridisation was completed, arrays were washed in buffers supplied by manufacture.

scan_protocol: Hybridized and washed slides were scanned using a GenePix 4000B Microarray Scanner (GE Healthcare) with a resolution of 10 μm pixel size.

data_processing: The background subtracted data was further analyzed with Acuity 4.0 (Molecular Devices) including normalization by a linear ratio-based method. For statistical significance, one sample t-test was applied with Bonferroni correction. For data analysis, a cut-off value of 1.0 (ln2) was used with p<0.09.

organism: Escherichia coli

source_name: strain 83972

label_protocol: 10 µg of total RNA primed with Primer Hexamers (Invitrogen) were reversed transcribed at 50°C for 1 h in the presence of 400 U SuperScript III RTase (Invitrogen), and 100 µM each dATP, dTTP, dGTP, with 30 µM dCTP, 70 µM Cy3-label or 70 µM Cy5-label

growth_protocol: For RNA isolation bacteria were grown at 37 °C in 125 ml of pooled human urine until the optical density OD600 reached 0.15.

extract_protocol: Total RNA extracted using RNAeasy (Qiagen) following manufacturer's instructions

treatment_protocol: Samples were treated with RNAprotect (Qiagen)

tag: protocol

value: Wild type strain grown until OD 0.15 in pooled human urine

tag: strain

value: re-isolate of strain ABU83972 from in vitro culture in pooled human urine after 68 days


title: PI-2 Replicate 2

description: Biological replicate 2 of 3. Culture of re-isolate PI-2 in pooled human urine harvested at 0.15 OD

source_name: strain 83972 PI-2

value: re-isolate of strain ABU83972 from paient PI after 214 days


title: 6475C3_cfa(1)

city: Houston

first: Delphine

last: Saulnier

state: TX

address: Feigin Center, Suite 830, 1102 Bates Avenue

department: Pathology

institute: Baylor College of Medicine

description: Biological replicate 1 of 3. Dye swap 1

hyb_protocol: For hybridization, yeast tRNA and salmon testes DNA were added to labeled cDNA along with a formamide hybridization buffer. Samples were applied to slides, and hybridization was carried out at 42 degrees Celsius in Corning microarray hybridization chambers overnight.  Slides were then washed sequentially for four minutes in each of the following buffers: 1X SSC and 0.2% SDS, 0.1X SSC and 0.2% SDS, and 0.1X SSC

scan_protocol: Arrays were scanned on a Axon instruments GenePix 4000B scanner.  Images were processed using GenePix Pro software (version 4.1)  and hybridization was carried out at 42 degrees Celsius in Corning microarray hybridization chambers overnight.  Slides were then washed sequentially for four minutes in each of the following buffers: 1X SSC and 0.2% SDS, 0.1X SSC and 0.2% SDS, and 0.1X SSC

data_processing: Data normalization between and within array using LOWESS

organism: Limosilactobacillus reuteri

source_name: Bacteria, wild-type

label_protocol: 2 ug of total RNA were primed with 2.5 ug of random hexamers (Qiagen) at 70 degrees Celsius for 10 minutes. Reverse transcription was then carried out using Superscript III (Invitrogen) with 500 uM each dATP, dCTP, and dGTP, 300 uM dTTP, and 200 uM aminoallyl dUTP. After reverse transcription, RNA was removed by hydrolysis and cDNA was cleaned. cDNA was then labeled using CyDye Post-labeling reactive dye packs (Amersham).

growth_protocol: Bacterial cells were grown for 24h in MRS at 37C in anaerobic conditions

extract_protocol: At the specified times, 5 ml of bacterial were collected and mixed with an equal part of ice-cold methanol. Cell pellets were centrifuged, washed, and resuspended in 0.25 units/ul mutanolysin. After incubation at 37 degrees Celsius for 20 minutes, total RNA was collected according to the manufacturer's instructions with the Qiagen RNeasy Kit.

value: ATCC PTA 6475

tag: genotype/variation

value: wild type

source_name: Bacteria, cfa mutant

value: cfa insertant


title: 6475C3_cfa(2)

description: Biological replicate 2 of 3. Dye swap 1


title: Isolated Schwann cells vs Schwann cells cocultured with neurons biological replicate 2 dye swap (NeuroDev2)

city: Paris

first: Stéphane

last: LE CROM

address: 46 rue d'Ulm

department: Biology Institute - IBENS

country: France

institute: École normale supérieure

description: Biological replicate two of two dye swap.

hyb_protocol: Microarrays were pre-hybridized during 1 hour at 42°C in 5x SSC, 0.1% SDS, 1% BSA, washed with distilled water and dried. They were hybridized overnight at 42°C in 25% formamide, 5x SSC, 0.1%SDS with Cy5- and Cy3-labelled cDNA, washed in 1x SSC, 0.2% SDS at 42°C, in 0.1x SSC, 0.2% SDS and two times in 0.1x SSC at room temperature and finally were spin-dried.

scan_protocol: The hybridized microarrays were scanned using Genepix 4000B (Molecular Devices, Sunnyvale, CA, USA) and the resulting image files analyzed by GenePix Pro 5.0 software (Axon).

data_processing: For each GenePix output file, two filters were applied, one to clear out spots and another one to discard saturating spots where the median foreground intensity was greater than 60,000 in one of the two channels. The resulting median foreground intensities were normalized, without background signal subtraction, using a global Lowess correction followed by a print-tip median normalization step (Lemoine et al., 2006).

molecule: polyA RNA

organism: Mus musculus

source_name: Isolated Schwann cells culture 4

label_protocol: The labelling of the RNA (5 ug) was carried out by incorporation of amino-allyl dUTP.

extract_protocol: mRNA was extracted using the micro scale RNA isolation kit (Ambion). Amplification of RNA samples for microarray experiments was achieved using the Amino Allyl MessageAmpTM aRNA kit (Ambion). Quantity and integrity of the RNA were measured with the RNA 6000 nano assay kit by the Agilent 2100 Bioanalyser (Agilent Technologies, Germany). All kits were used according to the manufacturer’s instructions.

treatment_protocol: B6D2 wild type and heterozygote Krox20GFP(DT)/+  (Vermeren al 2003) embryos were used for dorsal root ganglion (DRG) and Schwann Cells (SC) cultures respectively. DRG cultures: DRG were dissected from E17.5 embryos. After dissociation for 10 min at 37°C on a rocking table in Ca and Mg-free HBSS containing collagenase type I (130 U/ml), dispase I (2.5 mg/ml), followed by mechanical dissociation, cells were resuspended in DRG medium: DMEM supplemented with L-glutamin (2 mM), Horse serum (5%) and 2.5s NGF (50 ng/ml) (BD Biosciences), and plated onto 60 mm diameter dishes or 12 mm diameter CML coverslips that were coated with poly-L-lysine (PLL, 10 µg/ml, Sigma) and rat collagen type I (5 µg/cm2, Sigma). After 1 day, cytosine arabinoside (10 µM) was added for 2 days to the medium to get rid of non neuronal cells and cells were then maintained in DRG medium. SC cultures: Sciatic nerves were dissected from E17.5 embryos into ice-cold Ca, Mg-free HBSS and dissociated by incubating for 20 min at 37°C on a rocking table in Leibovitz medium (L15, Gibco) containing collagenase type I (130 U/ml) (Gibco), dispase I (2.5 mg/ml) (Roche), followed by mechanical dissociation. Cells were plated onto PLL and collagen coated dishes, and maintained in SC medium : 1:1 ratio of DMEM and Ham’s F12 (Gibco), 10% Fetal Calf Serum (FCS) supplemented with Na-selenite (0.5 ng/ml) (Sigma), progesterone (0.63 ng/ml) (Sigma), putrescine (1.6 µg/ml) (Sigma), transferring (0.1 mg/ml) (Sigma), insulin (0.5 µg/ml) (Sigma), forskolin (2 µM) (Sigma), heregulin (10 ng/ml) (Sigma), gentamycin (50 µg/ml), fungizone (2.5 µg/ml).  Indirect magnetic cell sorting: The mouse SC purification was performed using the technique of magnetic cell separation based on SC membrane expression of the low affinity Nerve Growth Factor receptor (p75NGFR) (Manent et al, 2003) according to manufacturer’s instructions (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany). Purity of SCs after magnetic cell sorting was quantified after immunolabelling as the percentage of p75NGFR- and S100- positive cells with respect to the total of cells counted. At least 100 cells per condition were counted and experiments were repeated 11 times. Results were expressed as mean ± SD, and statistical analysis was performed using Student’s t test. The purified population (enriched to 97 ± 2 % of SC, n=11) was either plated on coated dish or seeded onto DRG neurons and cocultures were then maintained for 1 to 4 days. Fluorescence activated cell sorting (FACS). Cells from SC-DRG cocultures were collected by trypsinisation and kept on ice in 0.1 M PBS containing 0,2% FCS. FITC fluorescent cells (GFP+ SCs) were sorted using a MoFlow cytometer (Becton Dickinson) and collected in lysis buffer (Ambion) to start the RNA extraction and to prevent any RNA degradation. An aliquot of cells isolated by FACS was preserved in 0.1 M PBS 0,2% FCS and cells were seeded for a couple of hours to allow them to attach to check cell sorting efficiency by immunocytochemistry using an anti-GFP antibody.

value: Isolated Schwann cells

tag: tissue

value: embryo

source_name: Schwann cells cocultured with neurons culture 4

value: Schwann cells cocultured with neurons


title: PI-2 Replicate 1

description: Biological replicate 1 of 3. Culture of re-isolate PI-2 in pooled human urine harvested at 0.15 OD


title: Ankle-Sphk1 norm-2

city: Charleston

first: DeAnna

last: Baker

state: SC

address: 114 Doughty Street STB #416

department: Medicine

institute: Medical University of South Carolina

description: Gene expression data from ankle joint of mouse that are wild-type with normal sphingosine kinase 1 activity; replicate 2

hyb_protocol: Samples were hybridized overnight to GeneChips in accordance with Affymetrix protocols.

scan_protocol: Post hybridization washing, staining and fluorescence scanning were performed using Affymetrix instrumentation in accordance with manufacturer recommendations.

data_processing: Scanned GeneChips (CEL files) were processed with Affymetrix Expression Console to obtain separate CHP files having RMA normalized expression values and MAS5 detection calls. RMA data and detection calls were merged manually and the composite data set was imported into dChip for comparison analysis.

source_name: ankle joint from a mouse at 5 months age

label_protocol: Total RNA (5 micrograms) was converted into biotin-labeled cRNA using  the SuperScript II cDNA synthesis kit (Invitrogen) and the Enzo HighYield in vitro transcription amplification kit (Enzo Life Sciences).

growth_protocol: Mice were reared and maintained using standard protocols

extract_protocol: RNA was isolated by phase separation with TRIzol reagent (Invitrogen) and further purified using RNAeasy kit (Qiagen).

treatment_protocol: Mice were anesthetized, euthanized, and ankle joints were removed by dissection.

tag: age

value: 5-month adult

tag: tnfa activity

value: normal

tag: sphingosine kinase 1 activity


title: PI-2 Replicate 3

description: Biological replicate 3of 3. Culture of re-isolate PI-2 in pooled human urine harvested at 0.15 OD


title: Ankle-hTNFa overexpression and Sphk1 norm-1

description: Gene expression data from ankle joint of mouse with hTNFa transgene and normal sphingosine kinase 1 activity; replicate 1

value: over-expression


title: BreastStroma_Tumor_E1426_replicate2

city: Montreal

first: Greg

last: Finak

state: QC

address: 3775 University

department: Mcgill Center For Bioinformatics

country: Canada

institute: McGill University

description: No additional description

hyb_protocol: Whole Human Genome 44K arrays (Agilent Technologies, product G4112A) were used for all experiments. RNA samples (500 ng) were subjected to fragmentation followed by 18 h hybridization, washing, and scanning (Agilent Technologies, model G2505B) according to the manufacturer’s protocol (manual ID #G4140-90030). Samples were hybridized against Universal Human Reference RNA (Stratagene, ID #740000, La Jolla, California, USA). Duplicate hybridizations were performed for all samples using reverse-dye labeling.

scan_protocol: Microarray data were feature extracted using Feature Extraction Software (v. 9.5.3) from Agilent with the default parameters.

data_processing:
Outlier features on arrays were flagged by the software. Arrays were required to have an average raw signal intensity of 800 in each channel, and a signal to noise ratio above 16 per channel. MvA plots were examined for signs of hybridization or labeling problems. Replicate arrays were required to have a concordance above 0.90. This level was established empirically using sets of known good replicate arrays in our database.
Data preprocessing and normalization were automated using the BIAS system. Raw feature intensities were background corrected using the RMA background correction algorithm. Resulting expression estimates were converted to log2-ratios. Within array normalization was performed using spatial and intensity-dependent loess. Median absolute deviation scale normalization was used to normalize between arrays.

source_name: Breast tumor-associated stroma (laser capture microdissected)

label_protocol: 2 to 4 ng of total RNA was subjected to two rounds of T7 linear amplification using Ambion Amino Allyl MessageAmp kit (Ambion, Austin, Texas, USA) and labeled with Cy3 and Cy5 dyes according to the manufacturer’s procedure. Prior to microarray hybridizations, amplified products were quantified using a spectrophotometer (Nanodrop, Wilmington, Delaware, USA) and subjected to BioAnalyzer to assay for quality (Agilent Technologies, Santa Clara, California, USA).

extract_protocol: Tissue specimens were microdissected into epithelium and stroma using a PixCell IIe LCM system (Arcturus). All microdissections were performed within three hours following tissue staining. Total RNA was extracted from each population of microdissected cells using a GITC (guanidinium isothiocyanate) extraction protocol. Briefly, LCM caps were incubated for 5 minutes (room temperature) in 200 μl GITC extraction buffer (4 M GITC, 25 mM sodium citrate pH 7.0, 0.1 M β-mercaptoethanol, 0.5% N-lauroylsarcosine) supplemented with 1.6 μl β-mercaptoethanol. Subsequently, 20 μl of 2 M NaOAc, pH 4.0, 220 μl of water-saturated phenol and 60 μl of chloroform-isoamyl alcohol (23:1) were added to the extraction buffer. Following 15 minutes incubation on ice and centrifugation (12,000 rpm, 15 minutes) the aqueous phase was removed and RNA was precipitated with 2 μl glycogen (GenHunter, Nashville, Tennessee, USA) and 200 μl isopropanol. Samples were placed at -80°C for 30 minutes and centrifuged at 4°C (12,000 rpm) for 30 minutes to pellet RNA. Pellets were washed with 70% ethanol, air dried and subjected to DNAseI treatment (Roche, Basel, Switzerland). DNAseI treatment was performed in the presence of an RNase inhibitor (Invitrogen, Carlsbad, California, USA). Subsequently, samples were re-extracted as described above and re-suspended in 10 μl of diethylpyrocarbonate-treated water. RNA was quantified using a RiboGreen assay (Molecular Probes, Carlsbad, California, USA).

tag: GRADE

value: III

value: Positive

tag: ER

value: Negative

tag: PR

value: Negative:

tag: Age

tag: Last Follow-Up

value: Alive With Disease

source_name: Reference

label_protocol: RNA was subjected to one round of T7 linear amplification using Ambion Amino Allyl MessageAmp kit (Ambion, Austin, Texas, USA) and labeled with Cy3 and Cy5 dyes according to the manufacturer’s procedure. Prior to microarray hybridizations, amplified products were quantified using a spectrophotometer (Nanodrop, Wilmington, Delaware, USA) and subjected to BioAnalyzer to assay for quality (Agilent Technologies, Santa Clara, California, USA).

extract_protocol: No special procedures were required to extract this material


title: BreastStroma_Tumor_E1426_replicate1

description: No additional description

tag: Universal Human Reference RNA (Stratagene, ID #740000, La Jolla, California, USA)


title: Ankle-hTNFa overexpression and SphK1 null-1

description: Gene expression data from ankle joint of mouse with hTNFa transgene and sphingosine kinase 1 functional null; replicate 1


title: HepG2 repeat 1

status: Public on Jan 14 2011

city: Beijing

first: Hongyan

last: Guo

address: Chengfu

department: School of Medcine

country: China

institute: Tsinghua University

description: low-molecuar-weight RNA derived from total RNA

hyb_protocol: Standard CapitalBio Methods: Labeled RNA was precipitated with 0.3 M sodium acetate, 2.5 volumes ethanol and resuspended in 15ul of hybridization buffer containing 3×SSC, 0.2% SDS and 15% formamide. The array was hybridized at 42℃ overnight and  washed with two consecutive washing solutions (0.2% SDS, 2×SSC at 42°C for 5 min, and 0.2% SSC for 5 min at room temperature).

scan_protocol: Luxscan 10K (CapitalBio)

data_processing: Intensity data was extracted from the TIFF images using GenePix Pro 4.0 (Axon Instruments, California, United States). Data processing including local background subtraction, quantile normalization across multiple slides was performed in R package (version 2.5.0).

label_protocol: 5μg of the LMW RNA was obtained and 3’ labeled with 500 ng of 5'-phosphate-cytidyl-uridyl-cy3-3' (Dharmacon, Chicago, United States) using T4 RNA ligase (NEB, Beijing, China) at 4°C for 4 hours in the dark. Eight synthetic small RNAs were spiked in for exogenous control.

growth_protocol: HepG2.2.15 cells were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum, 100U/ml penicillin, and 100U/ml streptomycin. Cells were maintained in minimal essential medium, supplemented with 1mM sodium pyruvate, nonessential amino acids, 2mM glutamine, 25μg/ml gentamicin, 100U/ml penicillin, 100U/ml streptomycin and 10% fetal bovine serum at 37°C with 5% CO2.

extract_protocol: Total RNA was extracted using Trizol reagent (Invitrogen) according to the manufacturer’s instructions. The low-molecular-weight (LMW) RNA was isolated from 35μg of total RNA using a PEG6000 solution precipitation method

treatment_protocol: No treatment


title: HepG2 repeat 2


title: HepG2 repeat 3


title: HepG2 repeat 5


title: HepG2 repeat 4


title: HepG2.2.15 repeat 6


title: LNCaP miR-616

city: Hong Kong

first: Stephanie

last: Ma

address: Rm 47, 1/F, Lab Block, Faculty of Medicine Bldg, 21 Sassoon Rd, Pok Fu Lam

department: School of Biomedical Sciences

country: Hong Kong

institute: The University of Hong Kong

description: gene expression

hyb_protocol: Service by University Health Network (UHN) Microarray Centre (standard Affymetrix protocol)

data_processing: The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method

source_name: LNCaP prostate cells, miR-616 stably overexpressed using lentivirus

growth_protocol: LNCaP cells grown in RPMI medium supplemented with 5% FBS and 1% penicillin and streptomycin

extract_protocol: Trizol extraction of total RNA was performed according to the manufacturer's instructions

treatment_protocol: LNCaP cells lentiviral transduced with miR-616 or empty vector control

value: Prostate

value: LNCaP prostate cells

value: miR-616 overexpression


title: Ankle-hTNFa overexpression and Sphk1 norm-2

description: Gene expression data from ankle joint of mouse with hTNFa transgene and normal sphingosine kinase 1 activity; replicate 2


title: HepG2 repeat 6


title: HepG2.2.15 repeat 1


title: HepG2.2.15 repeat 5


title: HepG2.2.15 repeat 3


title: HepG2.2.15 repeat 2


title: HepG2.2.15 repeat 4


title: Ankle-Sphk1 norm-1

description: Gene expression data from ankle joint of mouse that are wild-type with normal sphingosine kinase 1 activity; replicate 1


title: BreastStroma_Tumor_E1559_replicate1

description: No additional description

value: II

value: Positive:

value: Alive Without Disease


title: Control vs. Low-protein 239

city: Jerusalem

first: naomi

last: habib

address: Givat-Ram

department: Computer Scince

country: Israel

institute: Hebrew University

description: Biological replicate, dye-swap : effects of paternal low protein diet compared to control diet measured in mice offsprings

hyb_protocol: 20 μg of Cot-1 DNA (Gibco-BRL), 20 μg of polyA RNA (Sigma), and 20 μg of yeast tRNA (Gibco-BRL) were added as blocking agents, along with SSC (3.4X final), and SDS (0.3% final). Mixture was boiled for 3 min, then applied to home-printed “MEEBO” microarray. Microarrays were hybridized at 65 C for 16 hours

scan_protocol: Axon Genepix 4000B microarray scanner

data_processing: Arrays where normalized using block normalization procedure and between arrays normalization using a global average net signal intensity

source_name: Liver cells

label_protocol: 30 μg of total RNA was labeled for 2 hours at 42 C with Superscript II reverse transcriptase using 4 μg of random hexamer and 4 μg of oligo dT. Approximately half of samples were labeled with Cy3 for Control animal and Cy5 for Low Protein animal, and half were labeled in the opposite orientation. After labeling, reactions were stopped and RNA was degraded with NaOH, and Cy-labeled cDNA was isolated by MinElute cleanup. After cleanup of Cy3- and Cy5-labeled RNAs, samples were mixed together.

growth_protocol: At three weeks of age mice offspring were sacrificed by isoflurane and cervical dislocation, and median lobe of liver was rapidly dissected out and flash-frozen in liquid N2.

extract_protocol: Total RNA for microarray analysis was extracted from liver powder using Trizol.

treatment_protocol: male mice were weaned from mothers at 21 days of age, and sibling males were put into cages with low protein or control diet (moistened with water to allow mice to break the hard pellets).

tag: Sex

value: Male

tag: diet

value: Control

value: Low protein


title: MDA-MB231

source_name: human breast cancer cell line

growth_protocol: cells were cultured according to ATCC procotols.

tag: cell line

value: human breast cancer cell line


title: BreastStroma_Tumor_E1559_replicate2

description: No additional description


title: Low-protein vs. Control 240

description: Biological replicate: effects of paternal low protein diet compared to control diet measured in mice offsprings


title: Low-protein vs. Control 236


title: LNCaP EV

description: gene expression

scan_protocol: Service by University Health Network (UHN) Microarray Centre (standard Affymetrix protocol)

source_name: LNCaP prostate cells, empty lentivirus vector (control)

value: control


title: Low-protein vs. Control 243


title: Ankle-hTNFa overexpression and SphK1 null-3

description: Gene expression data from ankle joint of mouse with hTNFa transgene and sphingosine kinase 1 functional null; replicate 3

value: 5-month adult

value: over-expression


title: Control vs. Low-protein 99


title: Control vs. Low-protein 077


title: Control vs. Low-protein 235

value: Female


title: Control vs. Low-protein 245


title: Control vs. Low-protein 091


title: Control vs. Low-protein 089


title: Control vs. Low-protein 110


title: Low-protein vs. Control 115


title: Low-protein vs. Control 109


title: Control vs. Low-protein 093


title: Low-protein vs. Control 076


title: Ankle-hTNFa overexpression and SphK1 null-2

description: Gene expression data from ankle joint of mouse with hTNFa transgene and sphingosine kinase 1 functional null; replicate 2


title: Low-protein vs. Control 092


title: Control vs. Low-protein 096


title: Low-protein vs. Control 120


title: Low-protein vs. Control 238


title: Low-protein vs. Control 088


title: Control vs. Low-protein 073


title: Control vs. Low-protein 098


title: Low-protein vs. Control 097


title: IMR90 24 h control rep4

city: New York

first: Shanaz

middle: Adi

last: Ghandhi

state: NY

address: 630, W 168th street

department: Center for Radiological Research

institute: Columbia University Medical Center

hyb_protocol: 1.5 mg of cRNA (>9 pmol Cy3 per mg cRNA) was fragmented and hybridized to Agilent Whole Human Genome Oligo Microarrays (G4112F) using the Gene Expression Hybridization Kit as recommended by Agilent

scan_protocol: Slides were scanned immediately using the Agilent DNA Microarray Scanner (G2505B) and default parameters of Feature Extraction Software 9.1 (Agilent)

data_processing: Background corrected intensities were log2 transformed in BRB-ArrayTools, Version 3.8.1. Genes for which more than 10% of the data was either not above background or was a non-uniformity outlier were filtered out. Data are not normalized to preserve dependence across time points.

source_name: Lung fibroblast, control, 24.0 hr, rep4

label_protocol: Cyanine-3 (Cy3) labeled cRNA was prepared from 300 ng RNA using the One-Color Low RNA Input Linear Amplification PLUS kit (Agilent) according to the manufacturer’s instructions

growth_protocol: Early passage human lung fibroblast cell strain IMR-90 (Coriell repository, NJ) was sub-cultured in Dulbecco’s modified Eagle’s medium (Gibco) and Ham’s F10 medium in a 1:1 mixture plus 15% fetal bovine serum. Young fibroblasts at population doubling <35 were used in all experiments.

extract_protocol: RNA was isolated using Ribopure (Ambion, Applied Biosytems), according to the manufacturer’s instructions

treatment_protocol: Cells were exposed to 0 (sham irradiated) or 50 cGy 4He ions (125 keV per micron) using the track segment mode of the 5.5-MV Singletron accelerator

tag: cell type

value: primary lung fibroblast

tag: treatment

tag: time


title: IMR90 24 h control rep2

source_name: Lung fibroblast, control, 24.0 hr, rep2


title: IMR90 24 h control rep3

source_name: Lung fibroblast, control, 24.0 hr, rep3


title: OAW42 siRNA ADRM1(B) vs OAW42 nonspecific RNA (C)

city: Santa Monica

first: Charles

middle: L

last: Ginther

state: CA

address: 2825 Santa Monica Blvd, #200

department: Hem-Onc

institute: UCLA

hyb_protocol: Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential

scan_protocol: Scanned on an Agilent G2505C scanner.

data_processing:
Agilent Feature Extraction Software (v 9.5.1) was used for background subtraction and LOWESS normalization;: (1) local background used, (2) special detrend OFF, (3) adjust background globally OFF, (4) red autoestimate OFF, and (5) green autoestimate OFF.
Rosetta Resolver (v.7.2) was used for data analysis

source_name: QAW42 treated with siRNA to ADRM1 (B)

label_protocol: 10 µg of total RNA were primed with 2 µl of 100 µM T16N2 DNA primer at 70°C for 10 min, then reversed transcribed at 42°C for 1 h in the presence of 400 U SuperScript II RTase (Invitrogen), and 100 µM each dATP, dTTP, dGTP, with 25 µM dCTP, 25 µM Cy3-label

growth_protocol: Cells were cultured in DMEM medium plus 10% FBS and 4.5 g/L glucose.  Treatment: Used mix of four interfering RNAs specific for ADRM1 (from Dharmacon) as a Smartpool in addition to a non-targeting siRNA oligo to use as a control.  Transfection was performed using 0.4% Dharmafect transfection reagent and 10nM siRNA according to the manufacturer.  OAW42 cells were seeded in 6-well plates and transfected at 60% confluency in antibiotic-free DMEM medium supplemented with 1% L-glutamine, 0.91 ug/ml bovine insulin, 4.5g/1L glucose, and 3.7 g/L sodium bicarbonate). Treated for 48 hours.

extract_protocol: Total RNA extracted using Qiagen Rneasy Kit following manufacturer's instructions

tag: protocol

value: ADRM1 knockdown

source_name: OAW42 treated with nonspecific RNA

value: non-specific siRNA (control)


title: IMR90 24 h alpha rep4

source_name: Lung fibroblast, irradiated, 24.0 hr, rep4

value: irradiated


title: IMR90 24 h bystander rep3

source_name: Lung fibroblast, bystander, 24.0 hr, rep3

value: bystander


title: IMR90 6 h bystander rep4

source_name: Lung fibroblast, bystander, 6.0 hr, rep4


title: IMR90 24 h alpha rep1

source_name: Lung fibroblast, irradiated, 24.0 hr, rep1


title: IMR90 6 h bystander rep2

source_name: Lung fibroblast, bystander, 6.0 hr, rep2


title: IMR90 24 h alpha rep3

source_name: Lung fibroblast, irradiated, 24.0 hr, rep3


title: Low-protein vs. Control 90

tag: strain


title: IMR90 6 h control rep1

source_name: Lung fibroblast, control, 6.0 hr, rep1


title: IMR90 6 h bystander rep1

source_name: Lung fibroblast, bystander, 6.0 hr, rep1


title: IMR90 6 h alpha rep2

source_name: Lung fibroblast, irradiated, 6.0 hr, rep2


title: Low-protein vs. Control 124

source_name: Liver cells


title: IMR90 24 h bystander rep1

source_name: Lung fibroblast, bystander, 24.0 hr, rep1


title: IMR90 6 h alpha rep1

source_name: Lung fibroblast, irradiated, 6.0 hr, rep1


title: IMR90 6 h control rep4

source_name: Lung fibroblast, control, 6.0 hr, rep4


title: IMR90 6 h control rep2

source_name: Lung fibroblast, control, 6.0 hr, rep2


title: IMR90 24 h bystander rep4

source_name: Lung fibroblast, bystander, 24.0 hr, rep4


title: IMR90 6 h alpha rep3

source_name: Lung fibroblast, irradiated, 6.0 hr, rep3


title: IMR90 4 h bystander rep3

source_name: Lung fibroblast, bystander, 4.0 hr, rep3


title: IMR90 4 h bystander rep4

source_name: Lung fibroblast, bystander, 4.0 hr, rep4


title: IMR90 4 h alpha rep3

source_name: Lung fibroblast, irradiated, 4.0 hr, rep3


title: IMR90 2 h bystander rep4

source_name: Lung fibroblast, bystander, 2.0 hr, rep4


title: OAW42 siRNA ADRM1(B) vs OAW42 no added RNA (A)

source_name: OAW42 treated with siRNA to ADRM1 (B)

source_name: OAW42 untreated

value: untreated (control)


title: IMR90 4 h alpha rep1

source_name: Lung fibroblast, irradiated, 4.0 hr, rep1


title: IMR90 4 h alpha rep4

source_name: Lung fibroblast, irradiated, 4.0 hr, rep4


title: IMR90 2 h control rep1

source_name: Lung fibroblast, control, 2.0 hr, rep1


title: IMR90 24 h control rep1

source_name: Lung fibroblast, control, 24.0 hr, rep1


title: Low-protein vs. Control 095

source_name: Liver cells


title: IMR90 6 h bystander rep3

source_name: Lung fibroblast, bystander, 6.0 hr, rep3


title: IMR90 2 h bystander rep3

source_name: Lung fibroblast, bystander, 2.0 hr, rep3


title: IMR90 4 h control rep2

source_name: Lung fibroblast, control, 4.0 hr, rep2


title: IMR90 24 h alpha rep2

source_name: Lung fibroblast, irradiated, 24.0 hr, rep2


title: IMR90 4 h bystander rep2

source_name: Lung fibroblast, bystander, 4.0 hr, rep2


title: IMR90 2 h control rep3

source_name: Lung fibroblast, control, 2.0 hr, rep3


title: IMR90 6 h control rep3

source_name: Lung fibroblast, control, 6.0 hr, rep3


title: IMR90 4 h control rep3

source_name: Lung fibroblast, control, 4.0 hr, rep3


title: IMR90 2 h control rep4

source_name: Lung fibroblast, control, 2.0 hr, rep4


title: IMR90 4 h alpha rep2

source_name: Lung fibroblast, irradiated, 4.0 hr, rep2


title: IMR90 6 h alpha rep4

source_name: Lung fibroblast, irradiated, 6.0 hr, rep4


title: IMR90 2 h bystander rep1

source_name: Lung fibroblast, bystander, 2.0 hr, rep1


title: IMR90 2 h bystander rep2

source_name: Lung fibroblast, bystander, 2.0 hr, rep2


title: IMR90 24 h bystander rep2

source_name: Lung fibroblast, bystander, 24.0 hr, rep2


title: IMR90 4 h control rep1

source_name: Lung fibroblast, control, 4.0 hr, rep1


title: IMR90 2 h alpha rep4

source_name: Lung fibroblast, irradiated, 2.0 hr, rep4


title: IMR90 1 h control rep2

source_name: Lung fibroblast, control, 1.0 hr, rep2


title: IMR90 4 h bystander rep1

source_name: Lung fibroblast, bystander, 4.0 hr, rep1


title: IMR90 4 h control rep4

source_name: Lung fibroblast, control, 4.0 hr, rep4


title: IMR90 2 h control rep2

source_name: Lung fibroblast, control, 2.0 hr, rep2


title: IMR90 1 h alpha rep2

source_name: Lung fibroblast, irradiated, 1.0 hr, rep2


title: IMR90 2 h alpha rep2

source_name: Lung fibroblast, irradiated, 2.0 hr, rep2


title: IMR90 1 h bystander rep2

source_name: Lung fibroblast, bystander, 1.0 hr, rep2


title: IMR90 1 h alpha rep1

source_name: Lung fibroblast, irradiated, 1.0 hr, rep1


title: IMR90 2 h alpha rep1

source_name: Lung fibroblast, irradiated, 2.0 hr, rep1


title: IMR90 1 h alpha rep3

source_name: Lung fibroblast, irradiated, 1.0 hr, rep3


title: IMR90 1 h bystander rep4

source_name: Lung fibroblast, bystander, 1.0 hr, rep4


title: IMR90 1 h bystander rep3

source_name: Lung fibroblast, bystander, 1.0 hr, rep3


title: IMR90 1 h bystander rep1

source_name: Lung fibroblast, bystander, 1.0 hr, rep1


title: IMR90 0.5 h control rep2

source_name: Lung fibroblast, control, 0.5 hr, rep2


title: IMR90 0.5 h control rep3

source_name: Lung fibroblast, control, 0.5 hr, rep3


title: IMR90 1 h control rep3

source_name: Lung fibroblast, control, 1.0 hr, rep3


title: PE-2 bottom of the fermentor x PE-2 fermentation, Replicate 2

city: Ribeirão Preto

first: Patricia

middle: Alves

last: de Castro

state: São Paulo

address: Av. do Café

department: Pharmaceuticals Sciences

country: Brazil

institute: USP

description:
251632210139_GE2-v5_95_Feb07_1_3
Biological replicate 2 of 2.

hyb_protocol: For the hybridization, 825 ng of each labeled cRNA was mixed with Agilent Fragmentation Mix and incubated at 60 °C for exactly 30 minutes to fragment RNA. The fragmentation was interrupted by adding 55 µL of 2X GE Hybridization Buffer HI-RPM. Finally, 100 µL of sample was placed down onto the microarray slide, which was mounted into the Agilent Microarray Hybridization Chamber Kit. The hybridization was carried out in an oven (Agilent G2545A Hybridization Oven) set to 65 °C for 17 hours. After, microarray slides were washed according to Agilent’s instructions.

scan_protocol: Scanned using the GenePix 4000B microarray scanner (Molecular Devices, USA).

data_processing: Agilent Feature Extraction Software (v 9.5.3.1) was used for background subtraction and LOWESS normalization. After, data were processed using the TIGR platform. Data were visualized using TMeV from TIGR, and the same software was used for statistical analysis (t test).

organism: Saccharomyces cerevisiae

source_name: PE-2 bottom of the fermentor, labeled with Cyanine-3

label_protocol: Prior to labelling, cDNA was synthesized from 5 µg of RNA using Agilent's cDNA Master Mix. cRNA amplification and labeling were performed by adding to the samples the Agilent Transcription Master Mix plus Cyanine-3 or Cyanine-5 for 2 hours at 40 oC.

growth_protocol: The fed-batch fermentations with yeast cell recycle were conducted in bench scale reproducing all steps that occur in a typical industrial process of alcoholic fermentation in Brazil. Fermentations were carried out in triplicate for each strain. After centrifugation, the yeast cells were resuspended in water and fed with sugar cane must containing 18% sugars, being 50% from molasses and 50% from sugar cane juice. Fermentations were conducted at 33oC for 9 hours containing 10% of yeast cells (w / v). At the end of the first cycle, fermentative yeasts were centrifuged again and the cream of yeast treated with sulfuric acid to reach pH 2.5 for 1.5 hours at room temperature. After the acid treatment, yeast cells were fed with sugar cane must to start a new fermentation. In the second cycle, fermentation samples were collected for analysis of the transcriptome.

extract_protocol: Total RNA was extracted using Trizol following manufacturer's instructions, followed by DNAse treatment and purification using the RNeasy Mini Kit  (Qiagen).

treatment_protocol: The sampling of wine containing yeast cells was made in three steps: after acid treatment (before feeding), termination of must feeding (three hours) and at the end of fermentation (nine hours). In each step, 1 mL of sample was collected from each fermentation flask. The samples were immediately frozen in liquid nitrogen and stored at ultra low temperature (-70oC) for further analysis of the transcriptome.

tag: fermentation stage

value: bottom of the fermentor (before feeding)

source_name: PE-2 fermentation, labeled with Cyanine-5

value: fermentation


title: CAT bottom of the fermentor x CAT fermentation, Replicate 1

description:
251632210140_GE2-v5_95_Feb07_2_1
Biological replicate 1 of 2.

source_name: CAT bottom of the fermentor, labeled with Cyanine-3

value: CAT

source_name: CAT fermentation, labeled with Cyanine-5


title: CAT bottom of the fermentor x CAT fermentation, Replicate 2

description:
251632210139_GE2-v5_95_Feb07_2_3
Biological replicate 2 of 2.


title: CAT bottom of the fermentor x CAT feeding, Replicate 2

description:
251632210140_GE2-v5_95_Feb07_1_4
Biological replicate 2 of 2.

source_name: CAT feeding, labeled with Cyanine-5

value: feeding


title: IMR90 0.5 h bystander rep3

source_name: Lung fibroblast, bystander, 0.5 hr, rep3


title: IMR90 0.5 h bystander rep4

source_name: Lung fibroblast, bystander, 0.5 hr, rep4


title: PE-2 bottom of the fermentor x PE-2 fermentation, Replicate 1

description:
251632210138_GE2-v5_95_Feb07_2_3
Biological replicate 1 of 2.


title: PE-2 bottom of the fermentor x PE-2 feeding, Replicate 1

description:
251632210138_GE2-v5_95_Feb07_2_1
Biological replicate 1 of 2.

source_name: PE-2 feeding, labeled with Cyanine-5


title: IMR90 1 h alpha rep4

source_name: Lung fibroblast, irradiated, 1.0 hr, rep4


title: CAT bottom of the fermentor x CAT feeding, Replicate 1

description:
251632210137 A_GE2-v5_95_Feb07_2_1
Biological replicate 1 of 2.


title: IMR90 2 h alpha rep3

source_name: Lung fibroblast, irradiated, 2.0 hr, rep3


title: IMR90 0.5 h control rep1

source_name: Lung fibroblast, control, 0.5 hr, rep1


title: IMR90 0.5 h control rep4

source_name: Lung fibroblast, control, 0.5 hr, rep4


title: IMR90 0.5 h bystander rep1

source_name: Lung fibroblast, bystander, 0.5 hr, rep1


title: IMR90 1 h control rep1

source_name: Lung fibroblast, control, 1.0 hr, rep1


title: PE-2 bottom of the fermentor x PE-2 feeding, Replicate 2

description:
251632210139_GE2-v5_95_Feb07_1_1
Biological replicate 2 of 2.


title: IMR90 0.5 h alpha rep4

source_name: Lung fibroblast, irradiated, 0.5 hr, rep4


title: IMR90 1 h control rep4

source_name: Lung fibroblast, control, 1.0 hr, rep4


title: Day 10 First Chip

city: Bronx

first: Herb

last: Lachman

address: 1300 Morris Park Ave., F103

department: Psychiatry

institute: Albert Einstein College of Medicine

hyb_protocol: For gene expression profiling, an Affymetrix Human Gene 1.0 ST Array was used.

scan_protocol: Standard Affymetrix protocol

data_processing:
The raw intensity of each individual gene on the Affymetrix chips (CEL format) was processed and normalized by using the Robust multichip Average method (RMA) in the R language.
Data analyzed using the LIMMA algorithm.

source_name: Skin Fibroblast-derived  iPSCs

label_protocol: Standard Affymetrix protocol

growth_protocol:
Skin biopsy samples were transferred to a small Petri dish containing 2-3ml Skin Fibroblast Media (SFM) consisting of RPMI 1640, 10% FBS, 1% pen/strep, 10ng/ml FGF2. The sample was incubated at room temperature for 15 minutes. Medium was carefully aspirated and replaced with 1-2 mls of collagenase type II solution (3mg/ml collagenase II dissolved in DMEM High Glucose [Worthington Biochemical Corp. Lakewood, NJ: GIBCO/Invitrogen, Carlsbad, CA]). The tissue was chopped into small pieces using 2 sterile scalpels, after which they were allowed to incubate at 37oC for 12 hours depending on size. The sample was then collected in a 15ml falcon tube and washed with SFM (serum free medium). Tissue was collected by centrifugation at 1200 rpm for 4 minutes. The pelleted sample was then suspended in SFM and plated in a T12.5 ml flask at 37oC in 5% CO2 for 3 days without changing medium or manipulation, to allow fibroblasts to adhere. Then cells were fed every 2 days with RPMI 1640 containing 10% FBS until a confluent culture was obtained (~3 weeks).
Neuronal differentiation was induced in the following manner.  Briefly, iPSCs were treated with 1mg/ml dispase and detached using a cell scraper.  Colonies were pooled together and washed in hES medium and grown as cellular aggregates (embryoid bodies; EBs) on Ultra Low Adherence 6-well plates (Costar catalog # 3471, Corning, Corning, NY) without FGF2 (day 0). EBs were maintained with a daily medium change for 4 days.

extract_protocol: RNA samples used in the array passed quality control assessed by Agilent 2100 Bioanalyzer.

treatment_protocol: To support glutamatergic differentiation, adherent colonies were treated on day 10 with WNT3A (100ng/ml) (R&D Systems, Minneapolis, MN) in neural induction medium. Cells were fed every other day for 2 weeks. On day 25, medium was changed to neuronal differentiation medium, which consists of Neurobasal medium, 1X N2 supplement, 2X B27, 0.1mM NEAA, supplemented with cAMP (1 μM), BDNF, GDNF, and IGF1 (10ng/ml each, PeproTech, Rocky Hill, NJ).

tag: gender

value: female

tag: age

value: 29 years old

value: iPSCs

value: Day 10


title: IMR90 0.5 h alpha rep1

source_name: Lung fibroblast, irradiated, 0.5 hr, rep1


title: IMR90 0.5 h bystander rep2

source_name: Lung fibroblast, bystander, 0.5 hr, rep2


title: PE-2 bottom of the fermentor x PE-2 feeding, Replicate 2 (dye swap)

description:
251632210139_GE2-v5_95_Feb07_1_2
Dye-swap of biological replicate 2.

source_name: PE-2 bottom of the fermentor, labeled with Cyanine-5

source_name: PE-2 feeding, labeled with Cyanine-3


title: Day 32 First Chip

value: Day 32


title: OPMD Sample_O15

city: Leiden

first: Seyed Yahya

last: Anvar

address: Einthovenweg 20

department: Center for Human and Clinical Genetics

country: Netherlands

institute: Leiden University Medical Center

hyb_protocol: According to Illumina protocols

scan_protocol: According to Illumina protocols

data_processing: Before data analysis, microarray measurements were normalized to remove systematic errors by balancing the fluorescence intensities using the quantile method. Each time point has been normalized separately. Next, PCA plots were generated to assess the quality of the data. This analysis showed that 47% of the variations within each data set were attributed to the genetic variation between the WT and the transgenic mice. Subsequently, statistical analysis was conducted using limma package in R to identify genes with significant differences in expression pattern between A17.1 and WT. Statistical analysis includes a cut-off P-value of 0.05 and FDR correction provided in the limma package in R. Probe annotation was made with the Illumina mouse whole-genome bead array version 1 annotation package.

source_name: Quadricept

label_protocol: RNA labelling was performed with the Illumina® TotalPrep RNA Amplification kit (Ambion) according to the manufacturer's protocol, and subsequently was hybridized to Illumina Mouse v1.1 Bead arrays.

growth_protocol: A17.1 transgenic mice have previously been described. Male A17.1 mice and WT controls were generated by crossing the heterozygous carrier strain A17.1 obtained from Rubinsztein's group with the FvB background mice. The mice were genotyped by PCR 3–4 weeks after birth. Wild type FvB and A17.1 mice were housed in minimal disease facilities (Royal Holloway, University of London) with food and water ad libitum.

extract_protocol: Total RNA was extracted from skeletal muscles using RNA Bee (Amsbio) according to the manufacturer's instructions. RNA integration number (RIN) was determined with RNA 6000 Nano (Agilent Technologies). RNA with RIN >7 were used for subsequent steps.

tag: background strain

value: FvB

tag: genotype/variation

value: OPMD

value: Male

value: 26 week-old


title: Wild-Type Sample_W12

value: Wild-Type


title: Wild-Type Sample_W11


title: PE-2 bottom of the fermentor x PE-2 fermentation, Replicate 2 (dye swap)

description:
251632210139_GE2-v5_95_Feb07_1_4
Dye-swap of biological replicate 2.

source_name: PE-2 fermentation, labeled with Cyanine-3


title: Wild-Type Sample_W14


title: IMR90 0.5 h alpha rep2

source_name: Lung fibroblast, irradiated, 0.5 hr, rep2

value: primary lung fibroblast

value: irradiated


title: Wild-Type Sample_W15


title: Day 0 Second Chip

description:
control sample
8705.CEL

value: male

value: 23 years old

value: Day 0


title: OPMD Sample_O16


title: PE-2 bottom of the fermentor x PE-2 feeding, Replicate 1 (dye swap)

description:
251632210138_GE2-v5_95_Feb07_2_2
Dye-swap of biological replicate 1.

value: bottom of the fermentor (before feeding)


title: OPMD Sample_O17


title: Wild-Type Sample_W16


title: Day 10 Second Chip


title: Wild-Type Sample_W13


title: CAT bottom of the fermentor x CAT feeding, Replicate 1 (dye swap)

description:
251632210137 A_GE2-v5_95_Feb07_2_2
Dye-swap of biological replicate 1.

source_name: CAT bottom of the fermentor, labeled with Cyanine-5

value: CAT

source_name: CAT feeding, labeled with Cyanine-3

value: feeding


title: SPLEEN,FEMALE,CVB3,REP5

city: Baltimore

first: DeLisa

last: Fairweather

state: MD

address: 615 N Wolfe St

department: Environmental Health Sciences

institute: Johns Hopkins University

description: Gene expression data of female spleens after a 12 hour treatment of CVB3

hyb_protocol: Hybridization cocktails were prepared as recommended for arrays of Mini/169 format using reagents in the Affymetrix Hybridization Wash Stain kit. Hybridization mix and fragmented, labeled ST-sscDNA were incubated at 99oC for 2 min and 45oC for 5 min, and then centrifuged at maximum speed for 5 min prior to pipetting into the GeneChips (Affymetrix Mouse Gene ST 1.0). Hybridization was performed at 45oC for 18 hours at 60RPM in the Affymetrix rotisserie hybridization oven. The signal amplification protocol for washing and staining of eukaryotic targets was performed in an automated fluidies station (Affymetrix FS450) using Affymetrix protocol FS450_0001.

scan_protocol: Arrays were transferred to a GCS3000 laser scanner with autoloader and 3G upgrade (Affymetrix) and scanned at an emission wavelength of 570nm at 2.5 um resolution

data_processing:
CEL files were imported and analyzed with the default algorithm workflow RMA-Sketch, which includes RMA background correction, and Sketch-Quantile normalization.
The values recommended by Affymetrix as QC metrics include Positive vs. negative AUC (assessment of sensitivity and specificity), as well as all probeset mean and all probeset relative log expression.

source_name: FEMALE SPLEEN 12 HOURS POST CVB3 INFECTION

label_protocol: RNAs were processed for hybridization to Affymetrix Mouse Gene ST 1.0 microarrays using the Affymetrix GeneChip Whole Transcript Sense Target Labeling Assay, according to manufacturer's recommended protocol. One hundred nanograms of total RNA was amplified.

extract_protocol: Spleens were homogenized in 2mL TRIzol according to the manufacturer's protocol. PureLink Micro-to-midi Total RNA purification System was used for extraction and purification of RNA

treatment_protocol: Mice were treated with either PBS or heart-passaged CVB3 intraperitoneally. Mice were harvested at 12 hours post infection and spleens were flash frozen in liquid nitrogen for RNA extraction.

value: BALB/C

value: 8-10 weeks

value: Female

tag: agent

tag: tissue

value: spleen


title: PE-2 bottom of the fermentor x PE-2 fermentation, Replicate 1 (dye swap)

description:
251632210138_GE2-v5_95_Feb07_2_4
Dye-swap of biological replicate 1.

value: fermentation


title: SPLEEN,MALE,CVB3,REP4

description: Gene expression data of male spleens after a 12 hour treatment of CVB3

source_name: MALE SPLEEN 12 HOURS POST CVB3 INFECTION


title: CAT bottom of the fermentor x CAT fermentation, Replicate 2 (dye swap)

description:
251632210139_GE2-v5_95_Feb07_2_4
Dye-swap of biological replicate 2.

source_name: CAT fermentation, labeled with Cyanine-3


title: SPLEEN,MALE,CVB3,REP3


title: SPLEEN,MALE,CVB3,REP5


title: OPMD Sample_O12

value: FvB

value: OPMD


title: Wild-Type Sample_W9

value: Male

value: 18 week-old


title: SPLEEN,MALE,PBS,REP5

description: Gene expression data of male spleens after a 12 hour treatment of PBS

source_name: MALE SPLEEN 12 HOURS POST PBS INJECTION

value: control (PBS)


title: SPLEEN,FEMALE,CVB3,REP3


title: SPLEEN,FEMALE,CVB3,REP4


title: Wild-Type Sample_W8

value: Wild-Type


title: SPLEEN,FEMALE,CVB3,REP2


title: OPMD Sample_O14

value: 26 week-old


title: IMR90 0.5 h alpha rep3

source_name: Lung fibroblast, irradiated, 0.5 hr, rep3

value: primary lung fibroblast

value: irradiated


title: SPLEEN,FEMALE,CVB3,REP1


title: SPLEEN,MALE,CVB3,REP1


title: OPMD Sample_O13


title: Wild-Type Sample_W10


title: OPMD Sample_O9


title: Day 0 First Chip

description:
control sample
8509.CEL

source_name: Skin Fibroblast

value: female

value: 29 years old

value: iPSCs

value: Day 0


title: Wild-Type Sample_W7


title: SPLEEN,MALE,CVB3,REP2

value: BALB/C

value: 8-10 weeks


title: OPMD Sample_O11


title: Day 32 Second Chip

value: male

value: 23 years old

value: Day 32


title: OPMD Sample_O10

value: FvB

value: OPMD


title: SPLEEN,MALE,PBS,REP4

value: Male

value: spleen


title: OPMD Sample_O8

value: 18 week-old


title: Wild-Type Sample_W5

value: Wild-Type


title: Wild-Type Sample_W6


title: Cetuximab-resistant cell line, rep2

city: Nashville

first: Christine

middle: H.

last: Chung

state: TN

address: 777 PRB

department: Internal Medicine

institute: Vanderbilt University

description:
Gene expression data of 1Cc8_2.
940CC41-20080916-Hu133

hyb_protocol: Following fragmentation, the RNA was loaded on to the Affymetrix Human Genome U133 plus 2.0 GeneChip as directed (Affymetrix, Santa Clara, CA).

scan_protocol: GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 7G.

data_processing: The primary microarray data were normalized using RMA implemented in GeneSpring GX 10.0.2. The normalized data were then analyzed for differential expression between SCC1 and 1Cc8 cells.

source_name: cetuximab-resistant cells

label_protocol: The RNA was labeled with GeneChipR One-Cycle Target Labeling and Control Reagents.

growth_protocol: The SCC1 and 1Cc8 cell lines were grown in DMEM + 10% FBS + Hydrocortisone (1 μg/ml). Generation of 1Cc8 from SCC1 was previously described in Wheeler DL, et al. Oncogene 2008 (PMID 18297114).The cell lines were obtained from the University of Wisconsin.

extract_protocol: Total RNA was isolated from the cell lines using Qiagen RNeasy Mini kit as directed (Qiagen, Valencia, CA). The quality and quantity of the RNA was determined using the Agilent RNA 6000 NanoLabChip kit and Agilent 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA).

treatment_protocol: The cell lines were treated with cetuximab for 7 days at 100 nm, and growth inhibition was measured by MTS and colony formation assays. However, the treatment was only to determine the sensitivity. The array data were obtained from only the pre-treatment samples.

tag: cetuximab sensitivity

value: resistant


title: SPLEEN,FEMALE,PBS,REP3

description: Gene expression data of female spleens after a 12 hour treatment of PBS

source_name: FEMALE SPLEEN 12 HOURS POST PBS INJECTION

value: Female

value: control (PBS)


title: SPLEEN,FEMALE,PBS,REP5


title: Cetuximab-resistant cell line, rep1

description:
Gene expression data of 1Cc8_1.
940CC40-20080916-Hu133


title: SPLEEN,MALE,PBS,REP2


title: SPLEEN,FEMALE,PBS,REP4


title: Cetuximab-resistant cell line, rep3

description:
Gene expression data of 1Cc8_3.
940CC42-20080916-Hu133


title: SPLEEN,FEMALE,PBS,REP1


title: OPMD Sample_O5

value: 6 week-old


title: OPMD Sample_O6


title: OPMD Sample_O7


title: OPMD Sample_O4


title: SPLEEN,MALE,PBS,REP3


title: Sperm RNA in mice under Caloric restrictive diet H

description: Gene expression from Sperm of adult mice, under caloric restrictive diet

hyb_protocol: using the Affimetrix Hybridization, Wash and Stain Kit (according to manufacturer's instructions)

scan_protocol: Affymetrix 3000 7G GeneChip Scanner

data_processing: Data was normalized using RMA algorithm (Matlab)

source_name: Sperm

label_protocol: using the Affimetrix GeneChip Labeling Assay according to the manufacturer's instructions

growth_protocol: Sperm were isolated from caudal epididymus.

extract_protocol: After animals were sacrificed, caudal epididymus was dissected, punctured multiple times with needle-tipped tweezers, and incubated for 30 minutes in M2 media at 37 C. Supernatant was removed, pelleted (10,000g for 10 minutes), and washed 2X with PBS, then 1X in water. Sperm preparations were only used that were >99.5% pure as assessed by microscopy, and q-RT-PCR was also used to reject any sperm samples with detectable epididymus-specific genes Actb or Myh11

treatment_protocol: Females were weaned to control diet. Males were raised on diet (control or low-protein) until 9-12 weeks of age, at which point they were placed with females for one or two days.

value: Caloric Restriction


title: Sperm RNA in mice under Control diet G

description: Gene expression from Sperm of adult mice, under control diet

value: Control


title: Cetuximab-sensitive cell line, rep1

description:
Gene expression data of SCC1_1.
940CC37-20080916-Hu133

source_name: cetuximab-sensitive cells

value: sensitive


title: Wild-Type Sample_W2


title: wound_stressed_day6_rep3

city: Stanford

first: Michael

last: Januszyk

address: 257 Campus Dr.

department: Surgery

institute: Stanford University

hyb_protocol: Murine RNA was isolated, labeled, and hybridized to the GeneChip microarray according to the manufacturer’s protocols (Affymetrix, Santa Clara, CA, USA).

data_processing: Each gene in the microarray was represented by 20 oligonucleotide pairs, with each pair consisting of an oligonucleotide perfectly matched to the cDNA sequence, and a second oligonucleotide containing a single base mismatch. Raw microarray data (sample intensity files) were processed using GeneSpring GX 11.0 (Agilent Technologies Inc., Santa Clara, CA, USA). The normalization algorithm was lowess.

label: In situ oligonucleotide

source_name: Murine (C57BL/6 ) dorsal incisional wound tissue, stressed, day 6

extract_protocol: Murine tissue samples were harvested fromw ound sites at the indicated time points. The RNA extraction was performed with Trizol.

treatment_protocol: A murine model of hypertrophic scarring was applied, as described in:  Aarabi, S., et al. Mechanical load initiates hypertrophic scar formation through decreased cellular apoptosis. FASEB J 21, 3250-3261 (2007).

value: Dorsal incisional wound tissue


title: wound_stressed_day14_rep2

scan_protocol: Murine RNA was isolated, labeled, and hybridized to the GeneChip microarray according to the manufacturer’s protocols (Affymetrix, Santa Clara, CA, USA).

source_name: Murine (C57BL/6 ) dorsal incisional wound tissue, stressed, day 14


title: Wild-Type Sample_W3

source_name: Quadricept


title: Wild-Type Sample_W4


title: OPMD Sample_O3


title: Epididyma RNA in mice under low protein diet F

description: Gene expression from Epidydymus of adult mice, under low protein diet

source_name: Epididymus

value: Low Protein


title: Wild-Type Sample_W1


title: SPLEEN,FEMALE,PBS,REP2

value: BALB/C

value: 8-10 weeks


title: Sperm RNA in mice under low protein diet D

description: Gene expression from Sperm of adult mice, under low protein diet


title: OPMD Sample_O2

value: FvB


title: Cetuximab-sensitive cell line, rep2

description:
Gene expression data of SCC1_2.
940CC38-20080916-Hu133


title: Epididymal RNA in mice under Control diet E

description: Gene expression from Epidydymus of adult mice, under control diet


title: Sperm RNA in mice under Control diet B


title: wound_stressed_day6_rep4

label_protocol: Murine RNA was isolated, labeled, and hybridized to the GeneChip microarray according to the manufacturer’s protocols (Affymetrix, Santa Clara, CA, USA).


title: wound_stressed_day6_rep1


title: wound_stressed_day14_rep3


title: Sperm RNA in mice under Control diet A


title: SPLEEN,MALE,PBS, REP1


title: Sperm RNA in mice under low protein diet C


title: wound_stressed_day6_rep2


title: wound_unstressed_day6_rep1

source_name: Murine (C57BL/6 ) dorsal incisional wound tissue, unstressed, day 6


title: wound_stressed_day14_rep1


title: wound_unstressed_day14_rep3

source_name: Murine (C57BL/6 ) dorsal incisional wound tissue, unstressed, day 14


title: wound_unstressed_day6_rep4


title: wound_stressed_day14_rep4


title: wound_unstressed_day14_rep1


title: wound_unstressed_day14_rep4


title: OPMD Sample_O1

value: OPMD


title: Cetuximab-sensitive cell line, rep3

description:
Gene expression data of SCC1_3.
940CC39-20080916-Hu133


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 493696_AJ_F_gWAT

status: Public on Jan 13 2011

city: Seattle

first: Jonathan

middle: M

last: Derry

address: 1100 Fairview Ave N

institute: Sage Bionetworks

description: Derry J, Zhong H, Molony C et al ABSTRACT:  To identify the genes and pathways that underlie cardiovascular and metabolic phenotypes we performed an integrated analysis of a mouse C57BL/6J x A/J F2 (B6AF2) cross by relating genome-wide gene expression data from adipose, kidney, and liver tissues to physiological endpoints measured in the population. We have identified a large number of trait QTLs including loci driving variation in cardiac function on chromosomes 2 and 6 and a hotspot for adiposity, energy metabolism, and glucose traits on chromosome 8. Integration of adipose gene expression data identified a core set of genes that drive the chromosome 8 adiposity QTL. This chromosome 8 trans eQTL signature contains genes associated with mitochondrial function and oxidative phosphorylation and maps to a subnetwork with conserved function in humans that was previously implicated in human obesity. In addition, human eSNPs corresponding to orthologous genes from the signature show enrichment for association to type II diabetes in the DIAGRAM cohort, supporting the idea that the chromosome 8 locus perturbs a molecular network that in humans senses variations in DNA and in turn affects metabolic disease risk. We functionally validate predictions from this approach by demonstrating metabolic phenotypes in knockout mice for three genes from the trans eQTL signature, Akr1b8, Emr1, and Rgs2. In addition we show that the transcriptional signatures for knockout of two of these genes, Akr1b8 and Rgs2, map to the F2 network modules associated with the chromosome 8 trans eQTL signature and that these modules are in turn very significantly correlated with adiposity in the F2 population. Overall this study demonstrates how integrating gene expression data with QTL analysis in a network-based framework can aid in the elucidation of the molecular drivers of disease that can be translated from mice to humans.

hyb_protocol: Microarrays are incubated at 40°C for 48 hours in a rotating carousel.  Hybridizations to custom Agilent microarrays are completed as previously described (Hughes et al.Nat Biotech (2001), 19(4):342-7).  Microarrays are washed to remove non-specific hybridized sample. Afterwards, microarrays are dried in an ozone-free nitrogen chamber.

scan_protocol: Microarrays are scanned using the Agilent LP2 laser scanner. The scanner output is a Tiff file, which contains the quantitative hybridization data from each individual microarray. The Tiff files are then processed using Rosetta custom feature extraction software.

data_processing: Data were processed using the Rosetta Resolver® system. Rosetta's custom feature extraction software performs error modeling before data are loaded into the Resolver system. The Resolver system performs a squeeze operation that combines replicates of the same sequence in an array while applying error weighting. The error weighting consists of adjusting for additive and multiplicative noise. A P-value is generated and propagated throughout the system. The P-value represents the probability that a gene is expressed. The Resolver system allows users to set thresholds, below which genes of a P-value are considered to be significantly expressed. The Resolver system also combines multiple arrays using a squeezing process. If multiple spots reference one sequence, summarization is performed using an error-weighted average as described in Roland Stoughton and Hongyue Dai, Statistical Combining of Cell Expression Profiles. US Patent #6,351,712, February 26, 2002.

source_name: Adipose - gWAT

label_protocol: custom automated version of the aminoallyl MessageAmp II kit from Ambion.

extract_protocol: Qiagen RNeasy spin columns with DNAse treatment

tag: sample type

value: C57BL6/JxA/J F2 Control gWAT (n=200)

tag: animal id

value: A/J

value: F

tag: generation

value: Parental

value: adipose


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 490048_AJ_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 493698_AJ_M_gWAT

value: M


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 493694_AJ_F_gWAT


title: xrn4-5 flower 1

city: Newark

first: Linda

last: Rymarquis

state: DE

address: 15 Innovation way rm 241

institute: University of Delaware

description: Gene expression data from xrn4-5 inflorescence.

hyb_protocol: Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on the GeneChip ATH1 Array.

scan_protocol: GeneChips were scanned using the Hewlett-Packard GeneArray Scanner.

data_processing: Data were analyzed by the GCOS software package using Affymetrix default analysis settings and global scaling as the normalization method.

organism: Arabidopsis thaliana

source_name: mixed-stage flowers from xrn4-5

label_protocol: Biotinylated cRNA were prepared from 15 ug total RNA using the GeneChip IVT labeling kit from Affymetrix.

growth_protocol: Mixed-stage Arabidopsis inflorescences were collected from Col-0 and xrn4-5 plants grown under long day conditions (16 hr light, 8 hr dark) at 21C.

extract_protocol: Total RNA was extracted using the Qiagen RNeasy plant kit according to manufacturer's instructions.

treatment_protocol: Inflorescence tissue was harvested and frozen in liquid nitrogen.

tag: ecotype

tag: genotype

value: inflorescence

tag: stage

value: mixed


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 538668_AJ_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 493700_AJ_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 490054_AJ_M_gWAT


title: wound_unstressed_day6_rep3

value: Female


title: wound_unstressed_day14_rep2


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 490050_AJ_F_gWAT


title: wound_unstressed_day6_rep2


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 493695_AJ_F_gWAT


title: xrn4-5 flower 2


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 493699_AJ_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 538675_AJ_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 490041_B6_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 493697_AJ_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 490052_AJ_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 490040_B6_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 539014_B6_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 493674_B6_F_gWAT


title: Col-0 flower 2

description: Gene expression data from Col-0 inflorescence.

source_name: mixed-stage flowers from Col-0

value: wildtype


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 493677_B6_F_gWAT


title: Col-0 flower 1


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 539011_B6_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 490042_B6_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 490047_B6_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 493681_B6_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 539015_B6_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 493679_B6_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 493680_B6_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 490045_B6_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 490059_F1_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 493678_B6_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 493705_F1_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 490056_F1_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 539017_B6_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 539007_F1_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 538689_F1_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 539016_B6_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 539018_B6_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 538690_F1_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 490044_B6_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 493704_F1_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 490046_B6_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 493708_F1_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 538692_F1_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 490063_F1_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 493707_F1_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 539008_F1_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 538693_F1_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 493703_F1_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 490062_F1_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 538694_F1_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 552547_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 493706_F1_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 522489_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 516115_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 490058_F1_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 496912_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 490061_F1_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 491026_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 510615_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 512421_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 540125_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 522487_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 530414_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 491032_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 505331_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 522497_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 490060_F1_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 496907_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 549366_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 500866_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 510604_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 525913_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 491029_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 496910_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 522491_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 516124_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 500864_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 540133_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 496915_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 512412_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 516118_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 510611_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 512415_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 552553_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 510606_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 540130_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 540127_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 505329_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 510613_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 496911_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 522490_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 533793_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 505339_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 525919_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 516123_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 540129_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 549357_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 505332_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 510614_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 491034_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 538348_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 505340_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 540124_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 549367_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 516114_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 540134_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 525918_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 496913_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 533789_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 500886_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 491031_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 525920_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 491033_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 538345_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 530411_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 549348_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 510612_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 552550_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 491028_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 500868_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 552554_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 496909_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 533798_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 530413_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 505333_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 525912_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 496916_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 505336_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 540128_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 538344_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 538340_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 500885_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 505338_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 530419_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 552546_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 500869_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 516120_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 512411_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 530417_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 491037_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 525915_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 522492_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 533791_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 549358_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 512413_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 549352_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 512420_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 491035_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 496906_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 516117_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 512417_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 522494_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 525914_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 533792_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 516122_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 530422_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 538347_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 510609_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 505337_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 522493_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 500884_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 540126_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 552551_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 540131_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 496908_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 552556_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 533790_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 533797_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 522486_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 500865_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 510608_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 525910_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 512418_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 549353_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 500887_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 549349_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 538343_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 510610_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 533795_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 516116_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 533796_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 525916_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 491030_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 552552_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 522495_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 516119_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 549351_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 530415_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 496917_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 538337_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 525921_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 491042_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 512419_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 552560_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 530421_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 496926_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 510624_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 496927_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 538349_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 510607_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 540132_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 516132_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 540146_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 552569_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 530412_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 491036_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 533799_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 522501_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 516135_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 525924_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 491047_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 533808_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 505352_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 496919_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 491048_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 533803_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 512429_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 505345_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 533807_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 522508_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 510605_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 522500_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 533809_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 500883_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 510623_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 522509_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 512410_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 530427_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 525930_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 522505_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 530428_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 516121_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 538338_F2_F_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 540138_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 552565_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 552563_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 540141_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 500874_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 512427_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 540142_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 491039_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 540145_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 549354_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 512423_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 496921_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 496920_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 505341_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 510616_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 500872_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 549360_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 525932_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 512425_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 516134_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 516126_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 491043_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 552561_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 516131_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 533800_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 538355_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 552568_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 496929_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 491040_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 538351_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 496923_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 512424_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 500877_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 512432_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 500882_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 552558_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 505351_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 549362_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 533804_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 525927_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 512428_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 512433_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 505343_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 530425_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 549363_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 510627_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 505347_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 540144_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 530431_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 500880_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 491038_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 505342_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 500878_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 549364_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 522499_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 530423_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 538352_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 549371_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 540136_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 538356_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 525926_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 522507_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 496924_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 530430_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 510626_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 525928_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 552549_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 500873_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 510620_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 530429_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 500871_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 505348_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 516130_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 512431_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 549361_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 530433_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 533810_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 500876_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 522498_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 512422_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 538360_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 525933_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 530426_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 533801_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 496928_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 496918_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 510617_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 491049_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 552567_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 525929_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 505349_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 552559_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 512430_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 510625_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 538354_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 512426_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 525922_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 549365_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 491044_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 500881_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 538359_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 505346_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 505350_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 496922_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 533802_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 552562_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 500879_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 510618_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 510622_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 525925_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 516127_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 533811_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 552564_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 533805_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 491046_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 516125_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 540137_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 530434_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 525931_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 522502_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 549369_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 549355_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 491041_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 516136_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 540140_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 510621_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 516129_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 552566_F2_M_gWAT


title: Kidney Cortex F2 pool vs. 552567_F2_350

source_name: Kidney Cortex

value: Kidney Cortex F2 pool

value: kidney cortex


title: Kidney Cortex F2 pool vs. 552569_F2_352


title: Kidney Cortex F2 pool vs. 552568_F2_351


title: Kidney Cortex F2 pool vs. 552552_F2_355


title: Kidney Cortex F2 pool vs. 552565_F2_347


title: Kidney Cortex F2 pool vs. 552563_F2_346


title: Kidney Cortex F2 pool vs. 552549_F2_340


title: Kidney Cortex F2 pool vs. 552562_F2_345


title: Kidney Cortex F2 pool vs. 552551_F2_354


title: Kidney Cortex F2 pool vs. 552558_F2_341


title: Kidney Cortex F2 pool vs. 552547_F2_338


title: Kidney Cortex F2 pool vs. 549369_F2_334


title: Kidney Cortex F2 pool vs. 549367_F2_332


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 538357_F2_M_gWAT


title: Kidney Cortex F2 pool vs. 549365_F2_330


title: Kidney Cortex F2 pool vs. 549357_F2_325


title: Kidney Cortex F2 pool vs. 540143_F2_296


title: Kidney Cortex F2 pool vs. 549362_F2_324


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 522506_F2_M_gWAT


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 505344_F2_M_gWAT


title: Kidney Cortex F2 pool vs. 549348_F2_313


title: Kidney Cortex F2 pool vs. 549350_F2_315


title: Kidney Cortex F2 pool vs. 540142_F2_297


title: Kidney Cortex F2 pool vs. 549351_F2_316


title: Kidney Cortex F2 pool vs. 549366_F2_331


title: Kidney Cortex F2 pool vs. 540140_F2_305


title: Kidney Cortex F2 pool vs. 540141_F2_304


title: Kidney Cortex F2 pool vs. 540138_F2_312


title: Kidney Cortex F2 pool vs. 549352_F2_317


title: C57BL6/JxA/J F2 Control gWAT (n=200) vs. 491045_F2_M_gWAT


title: Kidney Cortex F2 pool vs. 540129_F2_301


title: Kidney Cortex F2 pool vs. 540132_F2_307


title: Kidney Cortex F2 pool vs. 540131_F2_303


title: Kidney Cortex F2 pool vs. 540133_F2_308


title: Kidney Cortex F2 pool vs. 540134_F2_309


title: Kidney Cortex F2 pool vs. 540130_F2_302


title: Kidney Cortex F2 pool vs. 540127_F2_298


title: Kidney Cortex F2 pool vs. 538355_F2_282


title: Kidney Cortex F2 pool vs. 540124_F2_289


title: Kidney Cortex F2 pool vs. 538354_F2_287


title: Kidney Cortex F2 pool vs. 538356_F2_281


title: Kidney Cortex F2 pool vs. 538359_F2_271


title: Kidney Cortex F2 pool vs. 540125_F2_290


title: Kidney Cortex F2 pool vs. 538352_F2_286


title: Kidney Cortex F2 pool vs. 538351_F2_280


title: Kidney Cortex F2 pool vs. 538350_F2_266


title: Kidney Cortex F2 pool vs. 538349_F2_265


title: Kidney Cortex F2 pool vs. 538347_F2_285


title: Kidney Cortex F2 pool vs. 538343_F2_278


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