title: Exp-2-Streptococcus pyogenes internalized in Detroit 562 human pharyngeal cells

hyb_protocol: cDNA probes labeled with Alexafluor 647(red test)and 555 (green control)were mixed and PCR purified. The labeled cDNA was hybridized with the array slide in the hybridization buffer ( 3x SSC, salmon testes (sonicated and denatured) DNA and 0.25% SDS) at 63C for 16h using HybChamber-Tm frm GeneMachine-Tm. The hybridized array slides were washed consecutively with 1xSSC, 0.2x SSC, and 0.05% SSC each with 0.3% SDS

extract_protocol: Total RNA from S. pyogenes was isolated by Qiagen RNAeasy kit.RNA was digested with RNAse-free DNAse and the RNA quality  was analyzed using nucleic acid bioanalyzed ( Agilent technologies).


title: [Control6]-[Healthy]-[CD4+T-cells]

status: Public on Jun 10 2011

type: genomic

city: Richmond

first: Mikhail

last: Dozmorov

state: VA

address: 830 E Main St

department: Biostatistics

country: USA

institute: Virginia Commonwealth University

description: Healthy

scan_protocol: Illumina iScan

data_processing:
The relative level of methylation for each CG site was calculated as the ratio of methylated-probe signal to total locus signal intensity, and defined within 0 to 1 range, exported from the Illumina BeadStudio software package.
The data were normalized as described previously, (Dozmorov I, Knowlton N, Tang Y, Shields A, Pathipvanich P, Jarvis JN, et al. Hypervariable genes--experimental error or hidden dynamics. Nucleic Acids Res 2004; Dozmorov I, Lefkovits I. Internal standard-based analysis of microarray data. Part 1: analysis of differential gene expressions. Nucleic Acids Res 2009) using the variability of areas with low methylation level as a reference point. In order to find genes with methylation level above the level of technical noise, a frequency histogram of raw methylation signal was determined for each array. The histogram yielded a right-skewed unimodal distribution curve with a mode of approximately 0.013.  A normal distribution curve representing the variability of the data around zero was then fitted around the mode, mirroring the Gaussian profile of the left part of the histogram. Its parameters were then defined (mean, SD) and the data were normalized to the standard deviation of the noise after subtraction of the mean. The data were then Log10-transformed and adjusted to each other by robust linear regression under the assumption that the methylation of most genes does not change. The data were then filtered to remove genes with a methylation level value less than 3.0, equivalent to setting a threshold at three standard deviations above the noise level. Genes with methylation differential below the noise level under all experimental conditions (about 1,600) were excluded from consideration, as their methylation cannot be reliably assessed.

molecule: genomic DNA

organism: Homo sapiens

source_name: CD4+T-cells, whole genomic DNA

label_protocol: Bisulfite-converted patient and control DNA samples were prepared and quantified using a NanoDrop scanning spectrophotometer (Thermo, Wilmington, DE).  For each sample, 500ng of whole-genome bisulfite-converted DNA was denatured, fragmented, and amplified using Illumina-supplied reagents according to manufacturer instructions.  Following precipitation, DNA was hybridized to Illumina Infinium HumanMethylation27 arrays, containing 50-mer oligonucleotides designed to hybridize either methylated (N-CG-N following bisulfite conversion) or unmethylated (N-TG-N following bisulfite conversion) cytosine on each CG pair interrogated, coupled to beads mounted on glass slides.  Microarrays were washed under high stringency, labeled with biotin (C and G nucleotides) or dinitrophenyl (A and T nucleotides), and scanned with an Illumina iScan.

growth_protocol: Twelve female lupus patients and 12 female controls were studied. The mean age was 42.83±3.61 in patients and 43.75±3.40 in controls (mean±SEM).

extract_protocol: CD4+ T lymphocytes were obtained via Ficoll/Paque PBMC enrichment (GE Healthcare Life Sciences, Piscataway, NJ) followed by CD4+ isolation using a MACS magnetic bead CD4+ T-cell isolation kit (Miltenyi Biosystems, Auburn, CA).  Whole genomic DNA was then prepared using a Qiagen DNEasy kit (Qiagen, Germantown, MD) and bisulfite-treated using a Zymo EZ DNA Methylation Kit (Zymo, Orange, CA).

treatment_protocol: Peripheral blood was collected from patient samples and controls.

tag: cell type

tag: disease status

value: healthy control


title: large airway [DGM-00249]

type: RNA

city: New York

first: Yael

last: Strulovici-Barel

state: NY

address: 1300 York Avenue

department: Department of Genetic Medicine

institute: Weill Cornell Medical College

description:
DGM-00249_lg.CHP
DGM-00249_lg.CEL

hyb_protocol: Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.

scan_protocol: GeneChips were scanned using the GeneChip Scanner 3000 7G.

data_processing: The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.

label: biotin

molecule: total RNA

source_name: large airway

label_protocol: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1-2 microg total RNA (Expression Analysis Technical Manual, 701022 Rev.2, Affymetrix).

extract_protocol: Trizol extraction and RNAeasy clean-up of total RNA was performed according to the manufacturer's instructions.

tag: department of genetic medicine id

tag: airway region

value: large airway

value: mixture of epithelium, basal, ciliated, secretory, undifferentiated and inflammatory cells

tag: time


title: [Case10]-[SLE]-[CD4+T-cells]

description: Case 10 : SLE 4 : Meds 0

hyb_protocol: DNA was hybridized to Illumina Infinium HumanMethylation27 arrays, containing 50-mer oligonucleotides designed to hybridize either methylated (N-CG-N following bisulfite conversion) or unmethylated (N-TG-N following bisulfite conversion) cytosine on each CG pair interrogated, coupled to beads mounted on glass slides.

label: biotin (C and G nucleotides) or dinitrophenyl (A and T nucleotides)

value: systemic lupus erythematosus

tag: sle disease activity index (sledai)


title: [Case8]-[SLE]-[CD4+T-cells]

description: Case 8 : SLE 4 : Meds 6


title: [Control2]-[Healthy]-[CD4+T-cells]

description: Healthy


title: [Control4]-[Healthy]-[CD4+T-cells]

description: Healthy


title: [Control3]-[Healthy]-[CD4+T-cells]

description: Healthy


title: [Case12]-[SLE]-[CD4+T-cells]

description: Case 12 : SLE 0 : Meds 8


title: [Case11]-[SLE]-[CD4+T-cells]

description: Case 11 : SLE 2 : Meds 2


title: Ethanol-limited chemostat, time 350h, rep2

city: Delft

first: Jean-Marc

last: Daran

address: Julianalaan 67

department: Department of Biotechnology

country: Netherlands

institute: Delft University of Technology

description: Ethanol-limited chemostat steady-state at time point 350h.

hyb_protocol: 15 µg of fragmented, biotinylated cRNA was hybridised to Affymetrix custom-made Penicillium chrysogenum GeneChip® microarrays (array code DSM_PENa520255F) at 45ºC for 16 h as described in the Affymetrix users' manual. Washing and staining of arrays were performed using the GeneChip® Fluidics Station 400.

scan_protocol: Scanning of the Affymetrix GeneChip microarrays was performed with the Affymetrix GeneArray Scanner 3000 following Affymetrix instructions. Acquisition and quantification of array images were performed using Affymetrix GeneChip Operating Software (GCOS version 1.2).

data_processing: Data were processed with the GeneChip Operating Software version 1.2 (GCOS 1.2) using Affymetrix default analysis settings and global scaling as normalization method. Before comparison, all arrays were globally scaled to a target value of 100 using the average signal from all gene features. To the 15,531 transcript features on the arrays, a filter was applied to extract 13,746 open reading frames of which there were 13,485 different genes. This discrepancy was due to several genes being represented more than once. To represent the variation in triplicate measurements, the coefficient of variation (S.D. divided by the mean) was calculated. When the genes were ranked according to increasing average intensity, the average coefficient of variation showed a sharp increase for the genes with the lowest expression. Therefore, all genes in which the average expression in all conditions was below 12 were removed from the dataset. Subsequently all remaining values below 12 were set to a value of 12. To assess differential expression, the Significance Analysis of Microarrays (SAM version 1.21) add-in to MicrosoftTM Excel was used for comparisons of replicate array experiments. SAM identifies genes with statistically significant changes in expression by assimilating a set of gene-specific t tests. Each gene is assigned a score on the basis of its change in gene expression relative to the standard deviation of repeated measurements for that gene. Genes with scores greater than a threshold are deemed potentially significant. The percentage of such genes identified by chance is the false discovery rate (FDR). To estimate the FDR, nonsense genes are identified by analyzing permutations of the measurements. The threshold can be adjusted to identify smaller or larger sets of genes, and FDRs are calculated for each set. Here internal SAM parameters for fold-change threshold and the false discovery rate values were set at 2 and 1% respectively. The genes with significantly changed expression in one of the comparisons were arranged in groups via overlapping them in Microsoft Excel.

label: Biotin

organism: Penicillium chrysogenum

source_name: Prolonged ethanol-limited chemostat sampled at 350h

label_protocol: Double-stranded cDNA synthesis was carried out using 10 µg of total RNA and the components of the One Cycle cDNA Synthesis Kit (Affymetrix, Santa Clara, USA). The double-stranded cDNA was purified with the GeneChip Sample Cleanup Module (Affymetrix/Qiagen) followed by in vitro transcription and labelling using the GeneChip IVT labeling Kit (Affymetrix). Finally, labelled cRNA was purified (GeneChip Sample Cleanup Module, Affymetrix/Qiagen) prior to fragmentation.

growth_protocol: Chemostat experiments were performed with the industrial high penicillin production strain Penicillium chrysogenum DS17690, kindly donated by DSM Anti-Infectives (Delft, The Netherlands). Cultivation medium was prepared as described earlier (van Gulik et al., 2000) and contained 0.25 Cmol/L ethanol and 4 mM PAA and was used for the batch and chemostat phase of fermentations, allowing a biomass concentration of about 3 gDW/L.

extract_protocol: For total RNA extraction, half of the pellet was grounded by mortar and pestle under constant cooling with liquid nitrogen. The powder was dissolved in 5 mL of Trizol reagent (Invitrogen) and 1 mL chloroform (Sigma) and mixed well. The two phases were separated by centrifugation (4600 g, 15 min). Total RNA was isolated using a phenol-chloroform extraction method, which consisted of two extraction steps in acid-phenol/chloroform/isoamyl alcohol (5:1, pH 4.8, Ambion, Foster City, USA), followed by a chloroform extraction step. Each time the phases were separated by centrifugation (4600 g, 15 min). Total RNA was precipitated for 30 min at -20ºC in 96% ethanol and 0.3 M sodium acetate. The RNA was collected by centrifugation at 23000 g for 15 min and dissolved in RNAse free H2O.

treatment_protocol:
Ethanol-limited chemostat cultivations were performed according to Nasution et al. (2006) in a 7 L turbine stirred reactor with a working volume of 4 L under aerobic conditions (DO > 70%) and a dilution rate of 0.03 h-1 at 25°C and pH 6.5. Sampling for transcriptome measurements, Western blotting and metabolome measurements was performed after about 75, 200, 350 and 500 h. 60 mL of culture broth was sampled and rapidly filtered over a glass fiber filter (Type A/E, Pall Life Sciences, East Hills, USA). The filter with mycelium was wrapped in aluminium foil, quenched in liquid nitrogen and stored at -80ºC until further use. Continued subcultivation of degenerated culture: From a nearly completely degenerated penicillin producing chemostat culture, a biomass sample was taken aseptically after about 500 hours of cultivation (15 residence times). Of this sample, four mL were used to inoculate a fermentor with 4L of fresh cultivation medium using a sterile syringe, which was cultivated (batch start-up followed by chemostat) under the exact same conditions as its parent culture after inoculation for 75h.

van Gulik WM, De Laat WTAM, Vinke JL, Heijnen JJ. 2000. Application of metabolic flux analysis for the identification of metabolic bottlenecks in the biosynthesis of penicillin-G. Biotechnol Bioeng 68: 602-618 (PMID: 10799985).

tag: strain

tag: treatment

value: ethanol limitation

tag: treatment time point


title: [Control1]-[Healthy]-[CD4+T-cells]

description: Healthy


title: Ethanol-limited chemostat, time 500h, rep1

description: Ethanol-limited chemostat steady-state at time point 500h.

source_name: Prolonged ethanol-limited chemostat sampled at 500h


title: [Case9]-[SLE]-[CD4+T-cells]

description: Case 9 : SLE 4 : Meds 3


title: [Case7]-[SLE]-[CD4+T-cells]

description: Case 7 : SLE 7 : Meds 5


title: Ethanol-limited chemostat, time 350h, rep1


title: [Control5]-[Healthy]-[CD4+T-cells]

description: Healthy


title: [Case4]-[SLE]-[CD4+T-cells]

description: Case 4 : SLE 4 : Meds 8


title: Ethanol-limited chemostat, time 500h, rep2


title: [Case3]-[SLE]-[CD4+T-cells]

description: Case 3 : SLE 6 : Meds 3


title: [Case5]-[SLE]-[CD4+T-cells]

description: Case 5 : SLE 8 : Meds 3


title: [Case1]-[SLE]-[CD4+T-cells]

description: Case 1 : SLE 10 : Meds 4


title: Ethanol-limited chemostat, time 75h, rep2

description: Ethanol-limited chemostat steady-state at time point 75h.

source_name: Prolonged ethanol-limited chemostat sampled at 75h


title: Ethanol-limited chemostat 500h subculture, time 75h, rep1

description: Sub-culture from 500h ethanol-limited chemostat steady-state at time point 75h.

source_name: Sub-culture from 500h ethanol-limited chemostat sampled at 75h


title: [Case6]-[SLE]-[CD4+T-cells]

description: Case 6 : SLE 8 : Meds 8


title: MH_3 [CH3'n]

city: Daejeon

first: Mirang

last: Kim

address: 52 Eoeun-dong

department: human genome center

country: South Korea

institute: KRIBB

description: replicate 3-3

hyb_protocol: bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol

scan_protocol: Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting

data_processing: BeadStudio software v3.2

label: Cy5 and Cy3

source_name: mature hepatocytes

label_protocol: Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays

growth_protocol: Human embryonic stem cells (CHA-hES4) were provided by the College of Medicine, Pochon CHA University, Seongnam, Korea.

extract_protocol: genomic DNA was extracted and purified using Qiagen DNeasy Kit according to standard instructions

treatment_protocol: Differentiation of ES into hepatocytes was carried out as described (Fig. 1A) (4). Briefly, ES were cultured to be confluent in conditioned medium on a feeder-free system for 3 days. Then, ES were incubated in RPMI-1640 (HyClone, Logan, UT) containing 0.5 mg/mL albumin fraction V (Sigma-Aldrich, St. Louis, MO) and 50 ng/mL activin A (Peprotech, Rocky Hill, NJ) for 1 day and further cultured in the same RPMI medium supplemented with 1% insulin-transferrin-selenium (ITS; Sigma-Aldrich) for 4 days. After treatment with activin A, EP were harvested. For further differentiation, EP were cultured in hepatocyte culture medium (HCM; Lonza, Baltimore, MD) containing 30 ng/mL FGF4 (Peprotech) and 20 ng/mL BMP2 (Peprotech) for 5 days and further incubated in HCM supplemented with 20 ng/mL hepatocyte growth factor (Peprotech) for 5 days. Maturation of ES-derived hepatocytes was induced by culturing in HCM supplemented with 10 ng/mL oncostatin M (R&D Systems, Minneapolis, MN) and 0.1 μM dexamethasone (Sigma-Aldrich) for 5 days. MH were harvested at this time point. The medium was changed daily.

tag: cell state of in vitro hepatogenesis

value: mature hepatocytes


title: mutant vs. wild type bio rep 2 tech rep 2

city: Wyndmoor

first: yanhong

last: Liu

state: PA

address: 600 E. Mermaid Lane

institute: ERRS-ARS-USDA

description: normalized log2 ratios of mutant/wild type

hyb_protocol: according to CustomArray 12K microarray protocols provided by Combimatrix

scan_protocol: at 5-um resolutions by the ScanArray ExpressHT microarray scanner

data_processing: normalized using median over entire array,  log2 of Red signal/ Green signal. BRB-ArrayTools software was used.

organism: Listeria monocytogenes

source_name: ctsR mutant cells

label_protocol: dye swap labeling using Alexa 555(cy3)  and Alexa 647 (cy5)

growth_protocol: Listeria monocytogenes Scott A mutant and wild type grown to O.D.600 0.7 to 0.8

extract_protocol: Total RNA extracted using Ambion RiboPure-Bacterial Kit following manufacture's instructions

treatment_protocol: Pressure treated (450 Mpa, 3 min)

tag: phenotype

value: ctsR mutant

source_name: wild type cells

value: wild type


title: mutant vs. wild type bio rep 2 tech rep 1


title: Ethanol-limited chemostat, time 75h, rep1


title: Ethanol-limited chemostat, time 200h, rep1

description: Ethanol-limited chemostat steady-state at time point 200h.

source_name: Prolonged ethanol-limited chemostat sampled at 200h


title: MH_2 [CH3'n]

description: replicate 3-2


title: mutant vs. wild type bio rep 1 tech rep 2


title: S. mutans clpX mutant replicate 1

city: Gainesville

first: Jessica

middle: K

last: Kajfasz

state: FL

address: 1395 Center Drive

department: Oral Biology

institute: University of Florida

description: clpX 1

hyb_protocol: The slides were hybridized to a mixture containing equal amounts of test and reference cDNA for 16 h at 42°C in a MAUI hybridization chamber (BioMicro Systems). Hybridized slides were washed and scanned using a GenePix scanner (Axon Instruments).

scan_protocol: Hybridized slides were washed and scanned using a GenePix scanner (Axon Instruments, Inc., Union City, CA).

data_processing: Data were analyzed using software available at the J. Craig Venter Institute PFGRC website. Single-channel images of the slides were loaded into Spotfinder and overlaid. A spot grid was created according to PFGRC specifications and manually adjusted to fit all spots. The Microarray Data Analysis System (MIDAS) software was used to normalize the spot intensity data using LOWESS and iterative log mean centering with default setting. MIDAS also utilized In-Slide Replicate Analysis to average the intensities of the spots that were replicated on the microarray slide. Spots that were missing or labeled as “bad” during the upstream processes in 50% of the slides were cut off in the output files, which also did not include control spots printed on the slides. Statistical analysis was carried out with BRB array tools (http://linus.nci.nih.gov/BRB-ArrayTools.html) with a cutoff P value of 0.001.

organism: Streptococcus mutans

source_name: clpX mid-log RNA

label_protocol: Once RNA was purified as described above, cDNA was generated from 2ug RNA per sample as described by the protocol available from the Pathogen Functional Genomics Research Center (PFGRC) using Invitrogen Superscript III Reverse Transcriptase (Invitrogen, Gaithersburg, MD) to increase cDNA yields. Purified cDNAs were coupled to Cy3 (experimental samples) or Cy5 (reference cDNA).

growth_protocol: Cells were grown in Brain-Heart medium to OD600 of 0.5 in a 5% CO2 incubator at 37°C.

extract_protocol: RNA was isolated from homogenized S. mutans cells grown in BHI medium buffered to pH 7.0 to OD600 of 0.4 by repeated hot acid-phenol: chloroform extractions, and the nucleic acid was precipitated with 1 volume ice-cold isopropanol and one-tenth volume 3M sodium acetate (pH 5) at –20°C overnight. RNA pellets were resuspended in 80 mL nuclease-free H2O, and treated with DNase I (Ambion) at 37°C for 30 minutes. RNA concentrations were determined using a Nanodrop ND-1000 Spectrophotometer (Thermo Fisher Scientific, Waltham, MA), and 1 mL samples were run on an agarose gel to verify RNA integrity. The RNA was purified again using the RNeasy mini kit (Qiagen), including a second on-column DNase treatment that was performed as recommended by the supplier. Final RNA concentrations were determined in triplicate.

tag: genotype

value: clpX mutant

tag: growth phase

value: mid-log

organism: Streptococcus mutans UA159

source_name: wild-type mid-log RNA


title: S. mutans clpX mutant replicate 3

description: clpX 3


title: S. mutans clpX mutant replicate 4

description: clpX 4


title: mutant vs. wild type bio rep 1 tech rep 1


title: S. mutans clpX mutant replicate 2

description: clpX 2


title: EP_3 [CH3'n]

description: replicate 2-3

source_name: endoderm progenitors

value: endoderm progenitors


title: Ethanol-limited chemostat, time 200h, rep2


title: S. mutans clpP mutant replicate 2

description: clpP 2

source_name: clpP mid-log RNA

value: clpP mutant


title: MH_1 [CH3'n]

description: replicate 3-1


title: S. mutans clpP mutant replicate 1

description: clpP 1


title: ES_2 [CH3'n]

description: replicate 1-2

source_name: Human embryonic stem cells (CHA-hES4)

value: embryonic stem cells


title: EP_1 [CH3'n]

description: replicate 2-1


title: S. mutans UA159 wild type replicate 4

description: UA159 4


title: S. mutans clpP mutant replicate 3

description: clpP 3


title: S. mutans clpP mutant replicate 4

description: clpP 4


title: S. mutans UA159 wild type replicate 1

description: UA159 1


title: EP_2 [CH3'n]

description: replicate 2-2


title: S. mutans UA159 wild type replicate 3

description: UA159 3


title: S. mutans UA159 wild type replicate 2

description: UA159 2


title: ES_3 [CH3'n]

description: replicate 1-3


title: Anther with trinucleate pollen, Biological replicate 1

city: New Delhi

first: Sanjay

last: Kapoor

state: Delhi

address: Benito Juarez Road

department: Department of Plant Molecular Biology

country: India

institute: University of Delhi South Campus

description: Gene expression data of anthers at trinucleate pollen stage

hyb_protocol: Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Arabidopsis Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450

scan_protocol: GeneChips were scanned using the Affymetrix Gene Chip Scanner 3300

data_processing: First-order analysis was performed using Affymetrix Gene Chip Operating (GCOS) software 1.2.1. Normalization of data and differential expression analysis was done using ArrayAssist ver 5.5  (Stratagene, CA, USA)

organism: Oryza sativa Indica Group

source_name: Anther with trinucleate pollen

label_protocol: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3ug total RNA

extract_protocol: Trizol extraction of total RNA was performed according to the manufacturer's instructions.

tag: variety

tag: tissue

value: Anthers at the the trinucleate stage of pollen

tag: anther length


title: ES_1 [CH3'n]

description: replicate 1-1


title: Anther with trinucleate pollen, Biological replicate 2


title: MH_4 [mRNA]

description: replicate 3-4

hyb_protocol: Standard Illumina hybridization protocol

scan_protocol: Standard Illumina scanning protocol

data_processing: The data were normalised using quantile normalisation with IlluminaGUI in R

extract_protocol: RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.


title: Anther with trinucleate pollen, Biological replicate 3


title: MH_3 [mRNA]

description: replicate 3-3


title: Anther with single celled pollen, Biological replicate 2

description: Gene expression data of anthers at single celled pollen stage

source_name: Anther with single celled pollen

value: Anthers at the uninucleate stage of microspore


title: MH_1 [mRNA]

description: replicate 3-1


title: 250908_UT-SCC-30

city: Helsinki

first: Ping

last: Chen

address: PO Box 63 (Haartmaninkatu 8), 00014 University of Helsinki, Finland

country: Finland

institute: Research Programs Unit, Genome-Scale Biology and Institute of Biomedicine, Biochemistry and Developmental Biology

description: Department of Otorhinolaryngology-Head and Neck Surgery at the Turku University Central Hospital (Turku, Finland).

hyb_protocol: Labeled cDNA was hybridized on Human Exon 1.0 ST microarray for 17 hours, washed and stained using Affymetrix Fluidics Station and scanned with Affymetrix GeneChip Scanner

scan_protocol: Array scanning was performed according to the manufacturer's instruction (Affymetrix)

data_processing: Exon array data analysis was performed using Multiple Exon Array Processing (MEAP) which includes Bayesian-based background correction, collective quantile normalization and data summarization. Annotation was done by aligning probes to the genome and then filtering probes to include only exonic probes for probesets or exons without multi-targets. MEAP can produce summarized data on probeset, exon, transcript or gene level.

source_name: HNSCC cell lines from tongue

label_protocol: cRNA template was hydrolyzed with RNase H leaving single-stranded cDNA. 5.5 ug of single-stranded cDNA was enzymatically fragmented and labeled with Affymetrix DNA Labeling Reagent

growth_protocol: Head and neck squamous cell carcinoma (HNSCC) cell lines from tongue UT-SCC-21, UT-SCC-24B, UT-SCC-30, UT-SCC-67, UT-SCC-73, UT-SCC-76A, UT-SCC-81, UT-SCC-87, UT-SCC-95 and larynx UT-SCC-8, UT-SCC-11, UT-SCC-75 were cultured in DMEM supplemented with L-glutamine and 10% fetal bovine serum at 37 degC in an atmosphere of 5% CO2. HNSCC cell lines SCC-4, SCC-9, SCC-25 were ordered from American Type Culture Collection (ATCC;Manassas, VA) and cultured according to the ATCC recommendations.

extract_protocol: RNA extraction was done by isolating total RNA using Rneasy mini kit from Qiagen

tag: cell line

tag: variation

value: HNSCC cell line without 11q13 amplification


title: Meiotic anther, Biological replicate 3

description: Gene expression data of meiotic anthers

source_name: Meiotic anther

value: Anthers undergoing meiotc division


title: 011208_UT-SCC-76A


title: 250908_UT-SCC-24B


title: 160309_SCC-9

description: American Type Culture Collection (ATCC; Manassas, VA)

source_name: HNSCC cell lines


title: 131008-UT-SCC-81


title: iPS MEF OSMPitx2

city: Kyoto

first: Shinya

last: Yamanaka

address: 53 Kawahara-machi, Shogoin, Sakyo-ku

country: Japan

institute: Center for iPS Cell Research and Applicaton (CiRA), Kyoto University

description: Nanog-iPS cells

hyb_protocol: Agilent standard protocol

scan_protocol: Agilent standard protocol

data_processing: Data were analyzed with GeneSpring GX 11. The parameters for normalization are as follows: Data transformation: Set measurement less than 0.01 to 0.01, Per chip: Normalize to 50th percentile

organism: Mus musculus

source_name: mouse embryonic fibroblasts, retroviral infection, iPS

label_protocol: Agilent standard protocol

growth_protocol: MEF were cultivated with 10% FBS/DMEM, passage by Trypsin.

extract_protocol: Total RNA was isolated with Trizol.

treatment_protocol: Transduction of indicated genes were done by retroviral infection.

tag: cell type

value: Nanog-iPS cells

tag: transduction


title: EP_1 [mRNA]

description: replicate 2-1


title: iPS MEF OSKM

description: Nanog-iPS cells

value: OSKM


title: 280409_UT-SCC-11

source_name: HNSCC cell lines from larynx


title: Meiotic anther, Biological replicate 2

description: Gene expression data of meiotic anthers


title: Anther with single celled pollen, Biological replicate 3


title: Day5 p53KO MEF OSKGlis1-2

description: Gene expression after 5 days retroviral transduction

source_name: p53-null mouse embryonic fibroblasts, retroviral infection, Day5

value: iPSC from embryonic fibroblast

value: p53 null


title: iPS MEF OSMGlis1

description: Nanog-iPS cells


title: Meiotic anther, Biological replicate 1

description: Gene expression data of meiotic anthers


title: EP_3 [mRNA]

description: replicate 2-3


title: ES_3 [mRNA]

description: replicate 1-3


title: Anther with single celled pollen, Biological replicate 1


title: Pre-meiotic anther, Biological replicate 1

description: Gene expression data of premeiotic anthers

source_name: Pre-meiotic anther

value: Anthers prior to meosis


title: iPS AHDF OSKM

description: iPS cells

data_processing: Data were analyzed with GeneSpring GX 11. The parameters for normalization are as follows: Data transformation: Set measurement less than 0.01 to 0.01, Per chip: Quantile normalization

source_name: adult human dermal fibroblast, retroviral infection, iPS

growth_protocol: AHDF were cultivated with 10% FBS/DMEM, passage by Trypsin.

value: iPSC from dermal fibroblast


title: Pre-meiotic anther, Biological replicate 3


title: Pre-meiotic anther, Biological replicate 2


title: EP_4 [mRNA]

description: replicate 2-4


title: MEF

description: Origin of iPS cells

source_name: mouse embryonic fibroblasts

value: mouse embryonic fibroblasts


title: EP_2 [mRNA]

description: replicate 2-2


title: ES_4 [mRNA]

description: replicate 1-4


title: MH_2 [mRNA]

description: replicate 3-2

source_name: mature hepatocytes

value: mature hepatocytes


title: 120309_UT-SCC-95


title: Day5 p53KO MEF OSK-2

value: embryonic fibroblast

value: OSK


title: iPS MEF OSMDmrtb1

description: Nanog-iPS cells


title: NG04528_NG04528

status: Public on Jun 09 2011

city: Cold Spring Harbor

first: Yoon-Ha

last: Lee

address: One Bungtown Road

institute: Cold Spring Harbor Lab

description:
test hybridization
NG04528

hyb_protocol: Microarrays were hybridized at 42°C for 72 hours, then washed according the standard Nimblegen HD2 protocol. For complete details, see Chapter 4 of the "NimbleGen Arrays User’s Guide" at https://wiki.cgb.indiana.edu/download/attachments/18186244/NimbleGen_CGH_Users_Guide_v3p1.pdf?version=2&modificationDate=1213279026000

scan_protocol: Microarrays were scanned at wavelengths of 532 (Cy3) and 635 (Cy5) nm, at a pixel size of 3 μm. For complete details, see Chapter 5 of the "NimbleGen Arrays User’s Guide" at https://wiki.cgb.indiana.edu/download/attachments/18186244/NimbleGen_CGH_Users_Guide_v3p1.pdf?version=2&modificationDate=1213279026000

data_processing:
Data were processed/analyzed using Matlab. Data were local- and Lowess-normalized, then 'system-normalized' by linear correction of test data with parameters derived from data containing no genetic signal. By utilizing singular value decomposition (SVD) of these 'self-self data,' the principal components of system noise were determined.
Two test/control ratios are presented: (1) “lowess_of_local_ratio” is the ratio after local and Lowess normalization of probe intensities and (2) “MPC_ratio” is the system normalized ratio for the “lowess_of_local_ratio”.

label_protocol: Samples were labeled by random-priming of genomic DNAs with Cy3 (sample) and Cy5 (reference) dye-labeled 9-mers. Two separate labeling reactions were used per hybridization. For complete details, see Chapter 3 of the "NimbleGenArrays User’s Guide" at https://wiki.cgb.indiana.edu/download/attachments/18186244/NimbleGen_CGH_Users_Guide_v3p1.pdf?version=2&modificationDate=1213279026000

extract_protocol: CHP-SKN-1 was purified from cultured skin fibroblasts by SDS/ProteinaseK digestion, phenol-chloroform extraction, and ethanol precipitation. REFM_1 was purified from immortalized lymphocytes using SDS/ProteinaseK digestion, phenol-chloroform extraction, and ethanol precipitation. REFM_R was purified from immortalized lymphocytes using the Gentra Autopure method on the Qiagen Autopure LS instrument according to the manufacturer’s instructions. All the test samples and CSHS0003 were purified from fresh blood (buffy coat fraction) using the Gentra Autopure method on the Qiagen Autopure LS instrument according to the manufacturer’s instructions. REF2M_C_1 and CSHA0D01 was purified from immortalized lymphocytes using the Gentra Autopure method on the Qiagen Autopure LS instrument according to the manufacturer’s instructions. CSHW0067 and CSHW0057 were purified from whole blood using Qiagen: QIAamp DNA Blood Maxi Kit.

tag: sample preparation

value: whole blood

value: blood

tag: experimental variable

value: test hybridization

value: cell culture

value: skin


title: NG04526_NG04526

description:
test hybridization
NG04526

value: EBV immortalized


title: NG04527_NG04527

description:
test hybridization
NG04527


title: ES_1 [mRNA]

description: replicate 1-1

value: embryonic stem cells


title: NG04521_NG04521

description:
test hybridization
NG04521


title: 131008_UT-SCC-73

value: HNSCC cell line without 11q13 amplification


title: NG04520_NG04520

description:
test hybridization
NG04520


title: NG04519_NG04519

description:
test hybridization
NG04519


title: 160309_SCC-25

value: HNSCC cell line with 11q13 amplification


title: NG04514_NG04514

description:
test hybridization
NG04514


title: NG04518_NG04518

description:
test hybridization
NG04518


title: NG04517_NG04517

description:
test hybridization
NG04517


title: 160309_UT-SCC-8


title: NG04512_NG04512

description:
test hybridization
NG04512


title: NG04513_NG04513

description:
test hybridization
NG04513


title: NG04516_NG04516

description:
test hybridization
NG04516


title: 310309_UT-SCC-67


title: AHDF

description: Origin of iPS cells

source_name: adult human dermal fibroblast

value: dermal fibroblast


title: NG04510_NG04510

description:
test hybridization
NG04510


title: NG04507_NG04507

description:
test hybridization
NG04507


title: NG04506_NG04506

description:
test hybridization
NG04506


title: iPS AHDF OSKGlis1

description: iPS cells


title: NG04504_NG04504

description:
test hybridization
NG04504


title: NG04524_NG04524

description:
test hybridization
NG04524


title: ES_2 [mRNA]

description: replicate 1-2


title: NG04522_NG04522

description:
test hybridization
NG04522


title: Day5 p53KO MEF OSKGlis1-1

value: iPSC from embryonic fibroblast

value: p53 null


title: 280409_SCC-4


title: 131008_UT-SCC-75


title: NG04525_NG04525

description:
test hybridization
NG04525


title: NG04500_NG04500

description:
test hybridization
NG04500


title: NG04497_NG04497

description:
test hybridization
NG04497


title: NG04523_NG04523

description:
test hybridization
NG04523


title: NG04515_NG04515

description:
test hybridization
NG04515


title: NG04509_NG04509

description:
test hybridization
NG04509


title: Day5 p53KO MEF OSK-1


title: NG04492_NG04492

description:
test hybridization
NG04492


title: NG04491_NG04491

description:
test hybridization
NG04491


title: NG04511_NG04511

description:
test hybridization
NG04511


title: NG04508_NG04508

description:
test hybridization
NG04508


title: NG04505_NG04505

description:
test hybridization
NG04505


title: 110509_UT-SCC-21


title: NG04499_NG04499

description:
test hybridization
NG04499


title: NG04503_NG04503

description:
test hybridization
NG04503


title: NG04485_NG04485

description:
test hybridization
NG04485


title: NG04495_NG04495

description:
test hybridization
NG04495


title: NG04487_NG04487

description:
test hybridization
NG04487


title: NG04501_NG04501

description:
test hybridization
NG04501


title: NG04496_NG04496

description:
test hybridization
NG04496


title: NG04502_NG04502

description:
test hybridization
NG04502


title: NG04489_NG04489

description:
test hybridization
NG04489


title: NG04494_NG04494

description:
test hybridization
NG04494


title: 011208_UT-SCC-87


title: NG04493_NG04493

description:
test hybridization
NG04493


title: NG04490_NG04490

description:
test hybridization
NG04490


title: NG04498_NG04498

description:
test hybridization
NG04498


title: NG04484_NG04484

description:
test hybridization
NG04484


title: NG04488_NG04488

description:
test hybridization
NG04488


title: NG04481_NG04481

description:
test hybridization
NG04481


title: NG04476_NG04476

description:
test hybridization
NG04476


title: NG04482_NG04482

description:
test hybridization
NG04482


title: NG04480_NG04480

description:
test hybridization
NG04480


title: NG04477_NG04477

description:
test hybridization
NG04477


title: NG04479_NG04479

description:
test hybridization
NG04479


title: NG04472_NG04472

description:
test hybridization
NG04472


title: NG04483_NG04483

description:
test hybridization
NG04483


title: NG04478_NG04478

description:
test hybridization
NG04478


title: NG04473_NG04473

description:
test hybridization
NG04473


title: NG04474_NG04474

description:
test hybridization
NG04474


title: NG04470_NG04470

description:
test hybridization
NG04470


title: NG04471_NG04471

description:
test hybridization
NG04471


title: NG04486_NG04486

description:
test hybridization
NG04486


title: NG04469_NG04469

description:
test hybridization
NG04469


title: NG04467_NG04467

description:
test hybridization
NG04467


title: NG04462_NG04462

description:
test hybridization
NG04462


title: NG04463_NG04463

description:
test hybridization
NG04463


title: NG04468_NG04468

description:
test hybridization
NG04468


title: NG04466_NG04466

description:
test hybridization
NG04466


title: NG04464_NG04464

description:
test hybridization
NG04464


title: NG04465_NG04465

description:
test hybridization
NG04465


title: NG04460_NG04460

description:
test hybridization
NG04460


title: NG04457_NG04457

description:
test hybridization
NG04457


title: NG04448_NG04448

description:
test hybridization
NG04448


title: NG04475_NG04475

description:
test hybridization
NG04475


title: NG04456_NG04456

description:
test hybridization
NG04456


title: NG04455_NG04455

description:
test hybridization
NG04455


title: NG04459_NG04459

description:
test hybridization
NG04459


title: NG04449_NG04449

description:
test hybridization
NG04449


title: NG04452_NG04452

description:
test hybridization
NG04452


title: NG04453_NG04453

description:
test hybridization
NG04453


title: NG04454_NG04454

description:
test hybridization
NG04454


title: NG04441_NG04441

description:
test hybridization
NG04441


title: NG04461_NG04461

description:
test hybridization
NG04461


title: NG04435_NG04435

description:
test hybridization
NG04435


title: NG04438_NG04438

description:
test hybridization
NG04438


title: NG04437_NG04437

description:
test hybridization
NG04437


title: NG04450_NG04450

description:
test hybridization
NG04450


title: NG04446_NG04446

description:
test hybridization
NG04446


title: NG04451_NG04451

description:
test hybridization
NG04451


title: NG04439_NG04439

description:
test hybridization
NG04439


title: NG04447_NG04447

description:
test hybridization
NG04447


title: NG04433_NG04433

description:
test hybridization
NG04433


title: NG04432_NG04432

description:
test hybridization
NG04432


title: NG04445_NG04445

description:
test hybridization
NG04445


title: NG04444_NG04444

description:
test hybridization
NG04444


title: NG04458_NG04458

description:
test hybridization
NG04458


title: NG04430_NG04430

description:
test hybridization
NG04430


title: NG04442_NG04442

description:
test hybridization
NG04442


title: NG04429_NG04429

description:
test hybridization
NG04429


title: NG04443_NG04443

description:
test hybridization
NG04443


title: NG04440_NG04440

description:
test hybridization
NG04440


title: NG04426_NG04426

description:
test hybridization
NG04426


title: NG04425_NG04425

description:
test hybridization
NG04425


title: NG04427_NG04427

description:
test hybridization
NG04427


title: NG04436_NG04436

description:
test hybridization
NG04436


title: NG04431_NG04431

description:
test hybridization
NG04431


title: NG04434_NG04434

description:
test hybridization
NG04434


title: NG04421_NG04421

description:
test hybridization
NG04421


title: NG04423_NG04423

description:
test hybridization
NG04423


title: NG04422_NG04422

description:
test hybridization
NG04422


title: NG04415_NG04415

description:
test hybridization
NG04415


title: NG04417_NG04417

description:
test hybridization
NG04417


title: NG04419_NG04419

description:
test hybridization
NG04419


title: NG04424_NG04424

description:
test hybridization
NG04424


title: NG04414_NG04414

description:
test hybridization
NG04414


title: NG04418_NG04418

description:
test hybridization
NG04418


title: NG04410_NG04410

description:
test hybridization
NG04410


title: NG04408_NG04408

description:
test hybridization
NG04408


title: NG04409_NG04409

description:
test hybridization
NG04409


title: NG04416_NG04416

description:
test hybridization
NG04416


title: NG04413_NG04413

description:
test hybridization
NG04413


title: NG04407_NG04407

description:
test hybridization
NG04407


title: NG04420_NG04420

description:
test hybridization
NG04420


title: NG04428_NG04428

description:
test hybridization
NG04428


title: NG04406_NG04406

description:
test hybridization
NG04406


title: NG04404_NG04404

description:
test hybridization
NG04404


title: NG04405_NG04405

description:
test hybridization
NG04405


title: NG04403_NG04403

description:
test hybridization
NG04403


title: NG04411_NG04411

description:
test hybridization
NG04411


title: NG04402_NG04402

description:
test hybridization
NG04402


title: NG04401_NG04401

description:
test hybridization
NG04401


title: NG04400_NG04400

description:
test hybridization
NG04400


title: NG04412_NG04412

description:
test hybridization
NG04412


title: NG04394_NG04394

description:
test hybridization
NG04394


title: NG04395_NG04395

description:
test hybridization
NG04395


title: NG04397_NG04397

description:
test hybridization
NG04397


title: NG04389_NG04389

description:
test hybridization
NG04389


title: NG04399_NG04399

description:
test hybridization
NG04399


title: NG04396_NG04396

description:
test hybridization
NG04396


title: NG04398_NG04398

description:
test hybridization
NG04398


title: NG04392_NG04392

description:
test hybridization
NG04392


title: NG04393_NG04393

description:
test hybridization
NG04393


title: NG04384_NG04384

description:
test hybridization
NG04384


title: NG04386_NG04386

description:
test hybridization
NG04386


title: NG04385_NG04385

description:
test hybridization
NG04385


title: NG04390_NG04390

description:
test hybridization
NG04390


title: NG04391_NG04391

description:
test hybridization
NG04391


title: NG04377_NG04377

description:
test hybridization
NG04377


title: NG04378_NG04378

description:
test hybridization
NG04378


title: NG04388_NG04388

description:
test hybridization
NG04388


title: NG04376_NG04376

description:
test hybridization
NG04376


title: NG04383_NG04383

description:
test hybridization
NG04383


title: NG04382_NG04382

description:
test hybridization
NG04382


title: NG04381_NG04381

description:
test hybridization
NG04381


title: NG04380_NG04380

description:
test hybridization
NG04380


title: NG04374_NG04374

description:
test hybridization
NG04374


title: NG04375_NG04375

description:
test hybridization
NG04375


title: NG04372_NG04372

description:
test hybridization
NG04372


title: NG04379_NG04379

description:
test hybridization
NG04379


title: NG04369_NG04369

description:
test hybridization
NG04369


title: NG04371_NG04371

description:
test hybridization
NG04371


title: NG04364_NG04364

description:
test hybridization
NG04364


title: NG04387_NG04387

description:
test hybridization
NG04387


title: NG04370_NG04370

description:
test hybridization
NG04370


title: NG04368_NG04368

description:
test hybridization
NG04368


title: NG04361_NG04361

description:
test hybridization
NG04361


title: NG04362_NG04362

description:
test hybridization
NG04362


title: NG04367_NG04367

description:
test hybridization
NG04367


title: NG04363_NG04363

description:
test hybridization
NG04363


title: NG04366_NG04366

description:
test hybridization
NG04366


title: NG04358_NG04358

description:
test hybridization
NG04358


title: NG04357_NG04357

description:
test hybridization
NG04357


title: NG04359_NG04359

description:
test hybridization
NG04359


title: NG04356_NG04356

description:
test hybridization
NG04356


title: NG04352_NG04352

description:
test hybridization
NG04352


title: NG04355_NG04355

description:
test hybridization
NG04355


title: NG04373_NG04373

description:
test hybridization
NG04373


title: NG04353_NG04353

description:
test hybridization
NG04353


title: NG04354_NG04354

description:
test hybridization
NG04354


title: NG04350_NG04350

description:
test hybridization
NG04350


title: NG04351_NG04351

description:
test hybridization
NG04351


title: NG04349_NG04349

description:
test hybridization
NG04349


title: NG04346_NG04346

description:
test hybridization
NG04346


title: NG04365_NG04365

description:
test hybridization
NG04365


title: NG04345_NG04345

description:
test hybridization
NG04345


title: NG04348_NG04348

description:
test hybridization
NG04348


title: CSHL0001_NG04156

description:
self-self hybridization
NG04156

value: self-self hybridization


title: CSHL0001_NG04159

description:
self-self hybridization
NG04159


title: NG04344_NG04344

description:
test hybridization
NG04344


title: CSHL0001_NG04158

description:
self-self hybridization
NG04158


title: NG04147_NG04147

description:
test hybridization
NG04147


title: NG04343_NG04343

description:
test hybridization
NG04343


title: NG04148_NG04148

description:
test hybridization
NG04148


title: CSHL0001_NG04157

description:
self-self hybridization
NG04157


title: NG04360_NG04360

description:
test hybridization
NG04360


title: NG04146_NG04146

description:
test hybridization
NG04146


title: NG04144_NG04144

description:
test hybridization
NG04144


title: NG04143_NG04143

description:
test hybridization
NG04143


title: NG04145_NG04145

description:
test hybridization
NG04145


title: NG04142_NG04142

description:
test hybridization
NG04142


title: NG04141_NG04141

description:
test hybridization
NG04141


title: NG04136_NG04136

description:
test hybridization
NG04136


title: NG04139_NG04139

description:
test hybridization
NG04139


title: NG04137_NG04137

description:
test hybridization
NG04137


title: NG04138_NG04138

description:
test hybridization
NG04138


title: NG04134_NG04134

description:
test hybridization
NG04134


title: NG04130_NG04130

description:
test hybridization
NG04130


title: CSHL0001_NG04160

description:
self-self hybridization
NG04160


title: NG04135_NG04135

description:
test hybridization
NG04135


title: NG04127_NG04127

description:
test hybridization
NG04127


title: NG04128_NG04128

description:
test hybridization
NG04128


title: NG04129_NG04129

description:
test hybridization
NG04129


title: NG04126_NG04126

description:
test hybridization
NG04126


title: NG04124_NG04124

description:
test hybridization
NG04124


title: NG04140_NG04140

description:
test hybridization
NG04140


title: NG04125_NG04125

description:
test hybridization
NG04125


title: NG04122_NG04122

description:
test hybridization
NG04122


title: NG04133_NG04133

description:
test hybridization
NG04133


title: NG04131_NG04131

description:
test hybridization
NG04131


title: NG04114_NG04114

description:
test hybridization
NG04114


title: NG04117_NG04117

description:
test hybridization
NG04117


title: NG04116_NG04116

description:
test hybridization
NG04116


title: NG04118_NG04118

description:
test hybridization
NG04118


title: NG04119_NG04119

description:
test hybridization
NG04119


title: NG04120_NG04120

description:
test hybridization
NG04120


title: NG04111_NG04111

description:
test hybridization
NG04111


title: NG04121_NG04121

description:
test hybridization
NG04121


title: NG04106_NG04106

description:
test hybridization
NG04106


title: NG04109_NG04109

description:
test hybridization
NG04109


title: NG04112_NG04112

description:
test hybridization
NG04112


title: NG04107_NG04107

description:
test hybridization
NG04107


title: NG04108_NG04108

description:
test hybridization
NG04108


title: NG04104_NG04104

description:
test hybridization
NG04104


title: NG04113_NG04113

description:
test hybridization
NG04113


title: NG04103_NG04103

description:
test hybridization
NG04103


title: NG04102_NG04102

description:
test hybridization
NG04102


title: NG04099_NG04099

description:
test hybridization
NG04099


title: NG04115_NG04115

description:
test hybridization
NG04115


title: NG04110_NG04110

description:
test hybridization
NG04110


title: NG04098_NG04098

description:
test hybridization
NG04098


title: NG04095_NG04095

description:
test hybridization
NG04095


title: NG04096_NG04096

description:
test hybridization
NG04096


title: NG04093_NG04093

description:
test hybridization
NG04093


title: NG04105_NG04105

description:
test hybridization
NG04105


title: NG04092_NG04092

description:
test hybridization
NG04092


title: NG04094_NG04094

description:
test hybridization
NG04094


title: NG04090_NG04090

description:
test hybridization
NG04090


title: NG04089_NG04089

description:
test hybridization
NG04089


title: NG04091_NG04091

description:
test hybridization
NG04091


title: NG04101_NG04101

description:
test hybridization
NG04101


title: NG04085_NG04085

description:
test hybridization
NG04085


title: NG04086_NG04086

description:
test hybridization
NG04086


title: NG04083_NG04083

description:
test hybridization
NG04083


title: NG04084_NG04084

description:
test hybridization
NG04084


title: NG04087_NG04087

description:
test hybridization
NG04087


title: NG04078_NG04078

description:
test hybridization
NG04078


title: NG04088_NG04088

description:
test hybridization
NG04088


title: NG04076_NG04076

description:
test hybridization
NG04076


title: NG04080_NG04080

description:
test hybridization
NG04080


title: NG04081_NG04081

description:
test hybridization
NG04081


title: NG04082_NG04082

description:
test hybridization
NG04082


title: NG04097_NG04097

description:
test hybridization
NG04097


title: NG04075_NG04075

description:
test hybridization
NG04075


title: NG04074_NG04074

description:
test hybridization
NG04074


title: NG04071_NG04071

description:
test hybridization
NG04071


title: NG04100_NG04100

description:
test hybridization
NG04100


title: NG04070_NG04070

description:
test hybridization
NG04070


title: NG04069_NG04069

description:
test hybridization
NG04069


title: NG04067_NG04067

description:
test hybridization
NG04067


title: NG04068_NG04068

description:
test hybridization
NG04068


title: NG04077_NG04077

description:
test hybridization
NG04077


title: NG04066_NG04066

description:
test hybridization
NG04066


title: NG04063_NG04063

description:
test hybridization
NG04063


title: NG04064_NG04064

description:
test hybridization
NG04064


title: NG04079_NG04079

description:
test hybridization
NG04079


title: NG04073_NG04073

description:
test hybridization
NG04073


title: NG04059_NG04059

description:
test hybridization
NG04059


title: NG04062_NG04062

description:
test hybridization
NG04062


title: NG04054_NG04054

description:
test hybridization
NG04054


title: NG04055_NG04055

description:
test hybridization
NG04055


title: NG04061_NG04061

description:
test hybridization
NG04061


title: NG04053_NG04053

description:
test hybridization
NG04053


title: NG04072_NG04072

description:
test hybridization
NG04072


title: NG04065_NG04065

description:
test hybridization
NG04065


title: NG04049_NG04049

description:
test hybridization
NG04049


title: NG04056_NG04056

description:
test hybridization
NG04056


title: NG04051_NG04051

description:
test hybridization
NG04051


title: NG04048_NG04048

description:
test hybridization
NG04048


title: NG04052_NG04052

description:
test hybridization
NG04052


title: NG04045_NG04045

description:
test hybridization
NG04045


title: NG04046_NG04046

description:
test hybridization
NG04046


title: NG04044_NG04044

description:
test hybridization
NG04044


title: NG04043_NG04043

description:
test hybridization
NG04043


title: NG04047_NG04047

description:
test hybridization
NG04047


title: NG04057_NG04057

description:
test hybridization
NG04057


title: NG04042_NG04042

description:
test hybridization
NG04042


title: NG04041_NG04041

description:
test hybridization
NG04041


title: NG04040_NG04040

description:
test hybridization
NG04040


title: NG04058_NG04058

description:
test hybridization
NG04058


title: NG04060_NG04060

description:
test hybridization
NG04060


title: NG04039_NG04039

description:
test hybridization
NG04039


title: NG04038_NG04038

description:
test hybridization
NG04038


title: NG04037_NG04037

description:
test hybridization
NG04037


title: NG04036_NG04036

description:
test hybridization
NG04036


title: NG04035_NG04035

description:
test hybridization
NG04035


title: NG04024_NG04024

description:
test hybridization
NG04024


title: NG04027_NG04027

description:
test hybridization
NG04027


title: NG04030_NG04030

description:
test hybridization
NG04030


title: NG04032_NG04032

description:
test hybridization
NG04032


title: NG04026_NG04026

description:
test hybridization
NG04026


title: NG04028_NG04028

description:
test hybridization
NG04028


title: NG04025_NG04025

description:
test hybridization
NG04025


title: NG04031_NG04031

description:
test hybridization
NG04031


title: NG04029_NG04029

description:
test hybridization
NG04029


title: NG04021_NG04021

description:
test hybridization
NG04021


title: NG04023_NG04023

description:
test hybridization
NG04023


title: NG04019_NG04019

description:
test hybridization
NG04019


title: NG04022_NG04022

description:
test hybridization
NG04022


title: NG04050_NG04050

description:
test hybridization
NG04050


title: NG04018_NG04018

description:
test hybridization
NG04018


title: NG04034_NG04034

description:
test hybridization
NG04034


title: NG04014_NG04014

description:
test hybridization
NG04014


title: NG04033_NG04033

description:
test hybridization
NG04033


title: NG04015_NG04015

description:
test hybridization
NG04015


title: NG04016_NG04016

description:
test hybridization
NG04016


title: NG04013_NG04013

description:
test hybridization
NG04013


title: NG04020_NG04020

description:
test hybridization
NG04020


title: NG04004_NG04004

description:
test hybridization
NG04004


title: NG04003_NG04003

description:
test hybridization
NG04003


title: NG04017_NG04017

description:
test hybridization
NG04017


title: NG04008_NG04008

description:
test hybridization
NG04008


title: NG04000_NG04000

description:
test hybridization
NG04000


title: NG04002_NG04002

description:
test hybridization
NG04002


title: NG04010_NG04010

description:
test hybridization
NG04010


title: NG04011_NG04011

description:
test hybridization
NG04011


title: NG04005_NG04005

description:
test hybridization
NG04005


title: NG03997_NG03997

description:
test hybridization
NG03997


title: NG03996_NG03996

description:
test hybridization
NG03996


title: NG03999_NG03999

description:
test hybridization
NG03999


title: NG03998_NG03998

description:
test hybridization
NG03998


title: NG04009_NG04009

description:
test hybridization
NG04009


title: NG03990_NG03990

description:
test hybridization
NG03990


title: NG03993_NG03993

description:
test hybridization
NG03993


title: NG03992_NG03992

description:
test hybridization
NG03992


title: NG04001_NG04001

description:
test hybridization
NG04001


title: NG03989_NG03989

description:
test hybridization
NG03989


title: NG03985_NG03985

description:
test hybridization
NG03985


title: NG03991_NG03991

description:
test hybridization
NG03991


title: NG03988_NG03988

description:
test hybridization
NG03988


title: NG04006_NG04006

description:
test hybridization
NG04006


title: NG04007_NG04007

description:
test hybridization
NG04007


title: NG03987_NG03987

description:
test hybridization
NG03987


title: NG03986_NG03986

description:
test hybridization
NG03986


title: NG03995_NG03995

description:
test hybridization
NG03995


title: NG03984_NG03984

description:
test hybridization
NG03984


title: NG03994_NG03994

description:
test hybridization
NG03994


title: NG03983_NG03983

description:
test hybridization
NG03983


title: NG03982_NG03982

description:
test hybridization
NG03982


title: NG03981_NG03981

description:
test hybridization
NG03981


title: NG03979_NG03979

description:
test hybridization
NG03979


title: NG03980_NG03980

description:
test hybridization
NG03980


title: NG03977_NG03977

description:
test hybridization
NG03977


title: NG04012_NG04012

description:
test hybridization
NG04012


title: NG03966_NG03966

description:
test hybridization
NG03966


title: NG03967_NG03967

description:
test hybridization
NG03967


title: NG03973_NG03973

description:
test hybridization
NG03973


title: NG03974_NG03974

description:
test hybridization
NG03974


title: NG03976_NG03976

description:
test hybridization
NG03976


title: NG03975_NG03975

description:
test hybridization
NG03975


title: NG03978_NG03978

description:
test hybridization
NG03978


title: NG03965_NG03965

description:
test hybridization
NG03965


title: NG03970_NG03970

description:
test hybridization
NG03970


title: NG03962_NG03962

description:
test hybridization
NG03962


title: NG03972_NG03972

description:
test hybridization
NG03972


title: NG03964_NG03964

description:
test hybridization
NG03964


title: NG03955_NG03955

description:
test hybridization
NG03955


title: NG03960_NG03960

description:
test hybridization
NG03960


title: NG03951_NG03951

description:
test hybridization
NG03951


title: NG03957_NG03957

description:
test hybridization
NG03957


title: NG03969_NG03969

description:
test hybridization
NG03969


title: NG03953_NG03953

description:
test hybridization
NG03953


title: NG03961_NG03961

description:
test hybridization
NG03961


title: NG03963_NG03963

description:
test hybridization
NG03963


title: NG03952_NG03952

description:
test hybridization
NG03952


title: NG03949_NG03949

description:
test hybridization
NG03949


title: NG03959_NG03959

description:
test hybridization
NG03959


title: NG03958_NG03958

description:
test hybridization
NG03958


title: NG03950_NG03950

description:
test hybridization
NG03950


title: NG03971_NG03971

description:
test hybridization
NG03971


title: NG03956_NG03956

description:
test hybridization
NG03956


title: NG03948_NG03948

description:
test hybridization
NG03948


title: NG03968_NG03968

description:
test hybridization
NG03968


title: NG03946_NG03946

description:
test hybridization
NG03946


title: NG03947_NG03947

description:
test hybridization
NG03947


title: NG03954_NG03954

description:
test hybridization
NG03954


title: NG03945_NG03945

description:
test hybridization
NG03945


title: NG03941_NG03941

description:
test hybridization
NG03941


title: NG03944_NG03944

description:
test hybridization
NG03944


title: NG03943_NG03943

description:
test hybridization
NG03943


title: NG03942_NG03942

description:
test hybridization
NG03942


title: NG03936_NG03936

description:
test hybridization
NG03936


title: NG03940_NG03940

description:
test hybridization
NG03940


title: NG03939_NG03939

description:
test hybridization
NG03939


title: NG03937_NG03937

description:
test hybridization
NG03937


title: NG03933_NG03933

description:
test hybridization
NG03933


title: NG03938_NG03938

description:
test hybridization
NG03938


title: NG03931_NG03931

description:
test hybridization
NG03931


title: NG03932_NG03932

description:
test hybridization
NG03932


title: NG03929_NG03929

description:
test hybridization
NG03929


title: NG03935_NG03935

description:
test hybridization
NG03935


title: NG03930_NG03930

description:
test hybridization
NG03930


title: NG03919_NG03919

description:
test hybridization
NG03919


title: NG03923_NG03923

description:
test hybridization
NG03923


title: NG03926_NG03926

description:
test hybridization
NG03926


title: NG03927_NG03927

description:
test hybridization
NG03927


title: NG03920_NG03920

description:
test hybridization
NG03920


title: NG03925_NG03925

description:
test hybridization
NG03925


title: NG03934_NG03934

description:
test hybridization
NG03934


title: NG03921_NG03921

description:
test hybridization
NG03921


title: NG03928_NG03928

description:
test hybridization
NG03928


title: NG03924_NG03924

description:
test hybridization
NG03924


title: NG03917_NG03917

description:
test hybridization
NG03917


title: NG03918_NG03918

description:
test hybridization
NG03918


title: NG03916_NG03916

description:
test hybridization
NG03916


title: NG03915_NG03915

description:
test hybridization
NG03915


title: NG03922_NG03922

description:
test hybridization
NG03922


title: NG03914_NG03914

description:
test hybridization
NG03914


title: NG03913_NG03913

description:
test hybridization
NG03913


title: NG03911_NG03911

description:
test hybridization
NG03911


title: NG03908_NG03908

description:
test hybridization
NG03908


title: NG03912_NG03912

description:
test hybridization
NG03912


title: NG03909_NG03909

description:
test hybridization
NG03909


title: NG03907_NG03907

description:
test hybridization
NG03907


title: NG03910_NG03910

description:
test hybridization
NG03910


title: NG03900_NG03900

description:
test hybridization
NG03900


title: NG03898_NG03898

description:
test hybridization
NG03898


title: NG03905_NG03905

description:
test hybridization
NG03905


title: NG03906_NG03906

description:
test hybridization
NG03906


title: NG03897_NG03897

description:
test hybridization
NG03897


title: NG03901_NG03901

description:
test hybridization
NG03901


title: NG03904_NG03904

description:
test hybridization
NG03904


title: NG03903_NG03903

description:
test hybridization
NG03903


title: NG03891_NG03891

description:
test hybridization
NG03891


title: NG03894_NG03894

description:
test hybridization
NG03894


title: NG03899_NG03899

description:
test hybridization
NG03899


title: NG03895_NG03895

description:
test hybridization
NG03895


title: NG03889_NG03889

description:
test hybridization
NG03889


title: NG03892_NG03892

description:
test hybridization
NG03892


title: NG03893_NG03893

description:
test hybridization
NG03893


title: NG03902_NG03902

description:
test hybridization
NG03902


title: NG03896_NG03896

description:
test hybridization
NG03896


title: NG03888_NG03888

description:
test hybridization
NG03888


title: NG03886_NG03886

description:
test hybridization
NG03886


title: NG03887_NG03887

description:
test hybridization
NG03887


title: NG03884_NG03884

description:
test hybridization
NG03884


title: NG03883_NG03883

description:
test hybridization
NG03883


title: NG03890_NG03890

description:
test hybridization
NG03890


title: NG03882_NG03882

description:
test hybridization
NG03882


title: NG03885_NG03885

description:
test hybridization
NG03885


title: NG03876_NG03876

description:
test hybridization
NG03876


title: NG03879_NG03879

description:
test hybridization
NG03879


title: NG03873_NG03873

description:
test hybridization
NG03873


title: NG03880_NG03880

description:
test hybridization
NG03880


title: NG03881_NG03881

description:
test hybridization
NG03881


title: NG03868_NG03868

description:
test hybridization
NG03868


title: NG03867_NG03867

description:
test hybridization
NG03867


title: NG03869_NG03869

description:
test hybridization
NG03869


title: NG03866_NG03866

description:
test hybridization
NG03866


title: NG03874_NG03874

description:
test hybridization
NG03874


title: NG03864_NG03864

description:
test hybridization
NG03864


title: NG03871_NG03871

description:
test hybridization
NG03871


title: NG03862_NG03862

description:
test hybridization
NG03862


title: NG03861_NG03861

description:
test hybridization
NG03861


title: CSHL0001_NG03834

description:
self-self hybridization
NG03834


title: NG03872_NG03872

description:
test hybridization
NG03872


title: NG03863_NG03863

description:
test hybridization
NG03863


title: NG03865_NG03865

description:
test hybridization
NG03865


title: CSHL0001_NG03835

description:
self-self hybridization
NG03835


title: CSHL0001_NG03832

description:
self-self hybridization
NG03832


title: CSHL0001_NG03833

description:
self-self hybridization
NG03833


title: NG03870_NG03870

description:
test hybridization
NG03870


title: NG03877_NG03877

description:
test hybridization
NG03877


title: NG03878_NG03878

description:
test hybridization
NG03878


title: CSHL0001_NG03831

description:
self-self hybridization
NG03831


title: CSHL0001_NG03830

description:
self-self hybridization
NG03830


title: NG03875_NG03875

description:
test hybridization
NG03875


title: CSHL0001_NG03829

description:
self-self hybridization
NG03829


title: CSHL0001_NG03818

description:
self-self hybridization
NG03818


title: CSHS0003_NG03828

description:
self-self hybridization
NG03828


title: CSHL0001_NG03820

description:
self-self hybridization
NG03820


title: CSHL0001_NG03822

description:
self-self hybridization
NG03822


title: CSHL0001_NG03819

description:
self-self hybridization
NG03819


title: CSHL0001_NG03556

description:
self-self hybridization
NG03556


title: CSHL0001_NG03817

description:
self-self hybridization
NG03817


title: CSHL0001_NG02896

description:
self-self hybridization
NG02896


title: CSHL0001_NG02895

description:
self-self hybridization
NG02895


title: CSHL0001_NG03399

description:
self-self hybridization
NG03399


title: CSHL0001_NG03398

description:
self-self hybridization
NG03398


title: CSHL0001_NG03821

description:
self-self hybridization
NG03821


title: CSHL0001_NG03592

description:
self-self hybridization
NG03592


title: CSHL0001_NG03555

description:
self-self hybridization
NG03555


title: CSHL0001_NG02894

description:
self-self hybridization
NG02894


title: CSHL0001_NG02897

description:
self-self hybridization
NG02897


title: NG02892_NG02892

description:
test hybridization
NG02892


title: NG02891_NG02891

description:
test hybridization
NG02891


title: NG02889_NG02889

description:
test hybridization
NG02889


title: CSHL0001_NG02883

description:
self-self hybridization
NG02883


title: CSHL0001_NG02871

description:
self-self hybridization
NG02871


title: CSHL0001_NG02870

description:
self-self hybridization
NG02870


title: NG02888_NG02888

description:
test hybridization
NG02888


title: CSHL0001_NG02843

description:
self-self hybridization
NG02843


title: D0015390_NG02844

description:
self-self hybridization
NG02844


title: NG02893_NG02893

description:
test hybridization
NG02893


title: CSHL0001_NG02724

description:
self-self hybridization
NG02724


title: CSHL0001_NG02721

description:
self-self hybridization
NG02721


title: CSHL0001_NG02722

description:
self-self hybridization
NG02722


title: CSHL0001_NG02880

description:
self-self hybridization
NG02880


title: CSHL0001_NG02718

description:
self-self hybridization
NG02718


title: CSHL0001_NG02719

description:
self-self hybridization
NG02719


title: CSHL0001_NG02717

description:
self-self hybridization
NG02717


title: CSHL0001_NG02723

description:
self-self hybridization
NG02723


title: CSHL0001_NG02712

description:
self-self hybridization
NG02712


title: CSHL0001_NG02713

description:
self-self hybridization
NG02713


title: CSHL0001_NG02709

description:
self-self hybridization
NG02709


title: CSHL0001_NG02711

description:
self-self hybridization
NG02711


title: CSHL0001_NG02714

description:
self-self hybridization
NG02714


title: CSHL0001_NG02715

description:
self-self hybridization
NG02715


title: CSHL0001_NG02708

description:
self-self hybridization
NG02708


title: CSHL0001_NG02562

description:
self-self hybridization
NG02562


title: CSHL0001_NG02707

description:
self-self hybridization
NG02707


title: CSHL0001_NG02551

description:
self-self hybridization
NG02551


title: CSHL0001_NG02539

description:
self-self hybridization
NG02539


title: CSHL0001_NG02527

description:
self-self hybridization
NG02527


title: CSHL0001_NG02515

description:
self-self hybridization
NG02515


title: CSHL0001_NG02826

description:
self-self hybridization
NG02826


title: CSHL0001_NG02491

description:
self-self hybridization
NG02491


title: CSHL0001_NG02012

description:
self-self hybridization
NG02012


title: CSHL0001_NG02503

description:
self-self hybridization
NG02503


title: GM10851_NG01957

description:
self-self hybridization
NG01957


title: CSHL0001_NG02720

description:
self-self hybridization
NG02720


title: GM10851_NG01956

description:
self-self hybridization
NG01956


title: GM10851_NG01955

description:
self-self hybridization
NG01955


title: CSHL0001_NG02710

description:
self-self hybridization
NG02710


title: CSHL0001_NG02716

description:
self-self hybridization
NG02716


title: GM10851_NG01954

description:
self-self hybridization
NG01954


title: GM10851_NG01910

description:
self-self hybridization
NG01910


title: CSHL0001_NG02563

description:
self-self hybridization
NG02563


title: GM10851_NG01953

description:
self-self hybridization
NG01953


title: GM10851_NG01909

description:
self-self hybridization
NG01909


title: GM10851_NG01906

description:
self-self hybridization
NG01906


title: GM10851_NG01907

description:
self-self hybridization
NG01907


title: NG07728_NG07728

description:
test hybridization
NG07728


title: NG07730_NG07730

description:
test hybridization
NG07730


title: NG07725_NG07725

description:
test hybridization
NG07725


title: NG07726_NG07726

description:
test hybridization
NG07726


title: NG07723_NG07723

description:
test hybridization
NG07723


title: NG07727_NG07727

description:
test hybridization
NG07727


title: NG07731_NG07731

description:
test hybridization
NG07731


title: NG07721_NG07721

description:
test hybridization
NG07721


title: NG07718_NG07718

description:
test hybridization
NG07718


title: NG07720_NG07720

description:
test hybridization
NG07720


title: NG07719_NG07719

description:
test hybridization
NG07719


title: NG07717_NG07717

description:
test hybridization
NG07717


title: NG07722_NG07722

description:
test hybridization
NG07722


title: NG07716_NG07716

description:
test hybridization
NG07716


title: NG07704_NG07704

description:
test hybridization
NG07704


title: NG07705_NG07705

description:
test hybridization
NG07705


title: NG07715_NG07715

description:
test hybridization
NG07715


title: GM10851_NG01908

description:
self-self hybridization
NG01908


title: NG07702_NG07702

description:
test hybridization
NG07702


title: NG07703_NG07703

description:
test hybridization
NG07703


title: NG07700_NG07700

description:
test hybridization
NG07700


title: NG07698_NG07698

description:
test hybridization
NG07698


title: NG07729_NG07729

description:
test hybridization
NG07729


title: NG07697_NG07697

description:
test hybridization
NG07697


title: NG07701_NG07701

description:
test hybridization
NG07701


title: NG07724_NG07724

description:
test hybridization
NG07724


title: NG07696_NG07696

description:
test hybridization
NG07696


title: NG07695_NG07695

description:
test hybridization
NG07695


title: NG07694_NG07694

description:
test hybridization
NG07694


title: NG07687_NG07687

description:
test hybridization
NG07687


title: NG07693_NG07693

description:
test hybridization
NG07693


title: NG07691_NG07691

description:
test hybridization
NG07691


title: NG07690_NG07690

description:
test hybridization
NG07690


title: NG07686_NG07686

description:
test hybridization
NG07686


title: NG07689_NG07689

description:
test hybridization
NG07689


title: NG07692_NG07692

description:
test hybridization
NG07692


title: NG07685_NG07685

description:
test hybridization
NG07685


title: NG07699_NG07699

description:
test hybridization
NG07699


title: NG07683_NG07683

description:
test hybridization
NG07683


title: NG07688_NG07688

description:
test hybridization
NG07688


title: NG07680_NG07680

description:
test hybridization
NG07680


title: NG07681_NG07681

description:
test hybridization
NG07681


title: NG07679_NG07679

description:
test hybridization
NG07679


title: NG07678_NG07678

description:
test hybridization
NG07678


title: NG07676_NG07676

description:
test hybridization
NG07676


title: NG07670_NG07670

description:
test hybridization
NG07670


title: NG07682_NG07682

description:
test hybridization
NG07682


title: NG07671_NG07671

description:
test hybridization
NG07671


title: NG07672_NG07672

description:
test hybridization
NG07672


title: NG07675_NG07675

description:
test hybridization
NG07675


title: NG07674_NG07674

description:
test hybridization
NG07674


title: NG07677_NG07677

description:
test hybridization
NG07677


title: NG07669_NG07669

description:
test hybridization
NG07669


title: NG07673_NG07673

description:
test hybridization
NG07673


title: NG07668_NG07668

description:
test hybridization
NG07668


title: NG07666_NG07666

description:
test hybridization
NG07666


title: NG07665_NG07665

description:
test hybridization
NG07665


title: NG07667_NG07667

description:
test hybridization
NG07667


title: NG07684_NG07684

description:
test hybridization
NG07684


title: NG07660_NG07660

description:
test hybridization
NG07660


title: NG07659_NG07659

description:
test hybridization
NG07659


title: NG07658_NG07658

description:
test hybridization
NG07658


title: NG07662_NG07662

description:
test hybridization
NG07662


title: NG07661_NG07661

description:
test hybridization
NG07661


title: NG07657_NG07657

description:
test hybridization
NG07657


title: NG07664_NG07664

description:
test hybridization
NG07664


title: NG07656_NG07656

description:
test hybridization
NG07656


title: NG07663_NG07663

description:
test hybridization
NG07663


title: NG07654_NG07654

description:
test hybridization
NG07654


title: NG07653_NG07653

description:
test hybridization
NG07653


title: NG07650_NG07650

description:
test hybridization
NG07650


title: NG07652_NG07652

description:
test hybridization
NG07652


title: NG07646_NG07646

description:
test hybridization
NG07646


title: NG07651_NG07651

description:
test hybridization
NG07651


title: NG07655_NG07655

description:
test hybridization
NG07655


title: NG07642_NG07642

description:
test hybridization
NG07642


title: NG07639_NG07639

description:
test hybridization
NG07639


title: NG07649_NG07649

description:
test hybridization
NG07649


title: NG07645_NG07645

description:
test hybridization
NG07645


title: NG07637_NG07637

description:
test hybridization
NG07637


title: NG07636_NG07636

description:
test hybridization
NG07636


title: NG07643_NG07643

description:
test hybridization
NG07643


title: NG07648_NG07648

description:
test hybridization
NG07648


title: NG07635_NG07635

description:
test hybridization
NG07635


title: NG07644_NG07644

description:
test hybridization
NG07644


title: NG07641_NG07641

description:
test hybridization
NG07641


title: NG07640_NG07640

description:
test hybridization
NG07640


title: NG07631_NG07631

description:
test hybridization
NG07631


title: NG07628_NG07628

description:
test hybridization
NG07628


title: NG07629_NG07629

description:
test hybridization
NG07629


title: NG07638_NG07638

description:
test hybridization
NG07638


title: NG07647_NG07647

description:
test hybridization
NG07647


title: NG07619_NG07619

description:
test hybridization
NG07619


title: NG07618_NG07618

description:
test hybridization
NG07618


title: NG07616_NG07616

description:
test hybridization
NG07616


title: NG07627_NG07627

description:
test hybridization
NG07627


title: NG07622_NG07622

description:
test hybridization
NG07622


title: NG07617_NG07617

description:
test hybridization
NG07617


title: NG07620_NG07620

description:
test hybridization
NG07620


title: NG07625_NG07625

description:
test hybridization
NG07625


title: NG07614_NG07614

description:
test hybridization
NG07614


title: NG07609_NG07609

description:
test hybridization
NG07609


title: NG07613_NG07613

description:
test hybridization
NG07613


title: NG07615_NG07615

description:
test hybridization
NG07615


title: NG07606_NG07606

description:
test hybridization
NG07606


title: NG07607_NG07607

description:
test hybridization
NG07607


title: NG07612_NG07612

description:
test hybridization
NG07612


title: NG07634_NG07634

description:
test hybridization
NG07634


title: NG07610_NG07610

description:
test hybridization
NG07610


title: NG07608_NG07608

description:
test hybridization
NG07608


title: NG07630_NG07630

description:
test hybridization
NG07630


title: NG07604_NG07604

description:
test hybridization
NG07604


title: NG07611_NG07611

description:
test hybridization
NG07611


title: NG07605_NG07605

description:
test hybridization
NG07605


title: NG07621_NG07621

description:
test hybridization
NG07621


title: NG07592_NG07592

description:
test hybridization
NG07592


title: NG07633_NG07633

description:
test hybridization
NG07633


title: NG07600_NG07600

description:
test hybridization
NG07600


title: NG07593_NG07593

description:
test hybridization
NG07593


title: NG07585_NG07585

description:
test hybridization
NG07585


title: NG07599_NG07599

description:
test hybridization
NG07599


title: NG07597_NG07597

description:
test hybridization
NG07597


title: NG07595_NG07595

description:
test hybridization
NG07595


title: NG07587_NG07587

description:
test hybridization
NG07587


title: NG07590_NG07590

description:
test hybridization
NG07590


title: NG07588_NG07588

description:
test hybridization
NG07588


title: NG07596_NG07596

description:
test hybridization
NG07596


title: NG07603_NG07603

description:
test hybridization
NG07603


title: NG07602_NG07602

description:
test hybridization
NG07602


title: NG07583_NG07583

description:
test hybridization
NG07583


title: NG07584_NG07584

description:
test hybridization
NG07584


title: NG07601_NG07601

description:
test hybridization
NG07601


title: NG07598_NG07598

description:
test hybridization
NG07598


title: NG07581_NG07581

description:
test hybridization
NG07581


title: NG07578_NG07578

description:
test hybridization
NG07578


title: NG07579_NG07579

description:
test hybridization
NG07579


title: NG07580_NG07580

description:
test hybridization
NG07580


title: NG07586_NG07586

description:
test hybridization
NG07586


title: NG07594_NG07594

description:
test hybridization
NG07594


title: NG07576_NG07576

description:
test hybridization
NG07576


title: NG07577_NG07577

description:
test hybridization
NG07577


title: NG07591_NG07591

description:
test hybridization
NG07591


title: NG07589_NG07589

description:
test hybridization
NG07589


title: NG07573_NG07573

description:
test hybridization
NG07573


title: NG07575_NG07575

description:
test hybridization
NG07575


title: NG07572_NG07572

description:
test hybridization
NG07572


title: NG07571_NG07571

description:
test hybridization
NG07571


title: NG07565_NG07565

description:
test hybridization
NG07565


title: NG07566_NG07566

description:
test hybridization
NG07566


title: NG07569_NG07569

description:
test hybridization
NG07569


title: NG07567_NG07567

description:
test hybridization
NG07567


title: NG07564_NG07564

description:
test hybridization
NG07564


title: NG07563_NG07563

description:
test hybridization
NG07563


title: NG07570_NG07570

description:
test hybridization
NG07570


title: NG07582_NG07582

description:
test hybridization
NG07582


title: NG07562_NG07562

description:
test hybridization
NG07562


title: NG07560_NG07560

description:
test hybridization
NG07560


title: NG07561_NG07561

description:
test hybridization
NG07561


title: NG07574_NG07574

description:
test hybridization
NG07574


title: NG07554_NG07554

description:
test hybridization
NG07554


title: NG07553_NG07553

description:
test hybridization
NG07553


title: NG07559_NG07559

description:
test hybridization
NG07559


title: NG07552_NG07552

description:
test hybridization
NG07552


title: NG07551_NG07551

description:
test hybridization
NG07551


title: NG07557_NG07557

description:
test hybridization
NG07557


title: NG07556_NG07556

description:
test hybridization
NG07556


title: NG07555_NG07555

description:
test hybridization
NG07555


title: NG07548_NG07548

description:
test hybridization
NG07548


title: NG07547_NG07547

description:
test hybridization
NG07547


title: NG07568_NG07568

description:
test hybridization
NG07568


title: NG07546_NG07546

description:
test hybridization
NG07546


title: NG07550_NG07550

description:
test hybridization
NG07550


title: NG07549_NG07549

description:
test hybridization
NG07549


title: NG07543_NG07543

description:
test hybridization
NG07543


title: NG07544_NG07544

description:
test hybridization
NG07544


title: NG07542_NG07542

description:
test hybridization
NG07542


title: NG07538_NG07538

description:
test hybridization
NG07538


title: NG07539_NG07539

description:
test hybridization
NG07539


title: NG07537_NG07537

description:
test hybridization
NG07537


title: NG07545_NG07545

description:
test hybridization
NG07545


title: NG07540_NG07540

description:
test hybridization
NG07540


title: NG07558_NG07558

description:
test hybridization
NG07558


title: NG07541_NG07541

description:
test hybridization
NG07541


title: NG07533_NG07533

description:
test hybridization
NG07533


title: NG07534_NG07534

description:
test hybridization
NG07534


title: NG07536_NG07536

description:
test hybridization
NG07536


title: NG07535_NG07535

description:
test hybridization
NG07535


title: NG07532_NG07532

description:
test hybridization
NG07532


title: NG07526_NG07526

description:
test hybridization
NG07526


title: NG07527_NG07527

description:
test hybridization
NG07527


title: NG07531_NG07531

description:
test hybridization
NG07531


title: NG07529_NG07529

description:
test hybridization
NG07529


title: NG07530_NG07530

description:
test hybridization
NG07530


title: NG07524_NG07524

description:
test hybridization
NG07524


title: NG07528_NG07528

description:
test hybridization
NG07528


title: NG07523_NG07523

description:
test hybridization
NG07523


title: NG07525_NG07525

description:
test hybridization
NG07525


title: NG07521_NG07521

description:
test hybridization
NG07521


title: NG07522_NG07522

description:
test hybridization
NG07522


title: NG07520_NG07520

description:
test hybridization
NG07520


title: NG07518_NG07518

description:
test hybridization
NG07518


title: NG07519_NG07519

description:
test hybridization
NG07519


title: NG07517_NG07517

description:
test hybridization
NG07517


title: NG07516_NG07516

description:
test hybridization
NG07516


title: NG07513_NG07513

description:
test hybridization
NG07513


title: NG07511_NG07511

description:
test hybridization
NG07511


title: NG07514_NG07514

description:
test hybridization
NG07514


title: NG07506_NG07506

description:
test hybridization
NG07506


title: NG07515_NG07515

description:
test hybridization
NG07515


title: NG07510_NG07510

description:
test hybridization
NG07510


title: NG07512_NG07512

description:
test hybridization
NG07512


title: NG07509_NG07509

description:
test hybridization
NG07509


title: NG07507_NG07507

description:
test hybridization
NG07507


title: NG07504_NG07504

description:
test hybridization
NG07504


title: NG07508_NG07508

description:
test hybridization
NG07508


title: NG07503_NG07503

description:
test hybridization
NG07503


title: NG07502_NG07502

description:
test hybridization
NG07502


title: NG07501_NG07501

description:
test hybridization
NG07501


title: NG07499_NG07499

description:
test hybridization
NG07499


title: NG07495_NG07495

description:
test hybridization
NG07495


title: NG07497_NG07497

description:
test hybridization
NG07497


title: NG07496_NG07496

description:
test hybridization
NG07496


title: NG07500_NG07500

description:
test hybridization
NG07500


title: NG07498_NG07498

description:
test hybridization
NG07498


title: NG07491_NG07491

description:
test hybridization
NG07491


title: NG07492_NG07492

description:
test hybridization
NG07492


title: NG07493_NG07493

description:
test hybridization
NG07493


title: NG07488_NG07488

description:
test hybridization
NG07488


title: NG07490_NG07490

description:
test hybridization
NG07490


title: NG07489_NG07489

description:
test hybridization
NG07489


title: NG07505_NG07505

description:
test hybridization
NG07505


title: NG07487_NG07487

description:
test hybridization
NG07487


title: NG07486_NG07486

description:
test hybridization
NG07486


title: NG07441_NG07441

description:
test hybridization
NG07441


title: NG07442_NG07442

description:
test hybridization
NG07442


title: NG07438_NG07438

description:
test hybridization
NG07438


title: NG07440_NG07440

description:
test hybridization
NG07440


title: NG07626_NG07626

description:
test hybridization
NG07626


title: NG07436_NG07436

description:
test hybridization
NG07436


title: NG07439_NG07439

description:
test hybridization
NG07439


title: NG07434_NG07434

description:
test hybridization
NG07434


title: NG07435_NG07435

description:
test hybridization
NG07435


title: NG07437_NG07437

description:
test hybridization
NG07437


title: NG07494_NG07494

description:
test hybridization
NG07494


title: NG07432_NG07432

description:
test hybridization
NG07432


title: NG07433_NG07433

description:
test hybridization
NG07433


title: NG07427_NG07427

description:
test hybridization
NG07427


title: NG07428_NG07428

description:
test hybridization
NG07428


title: NG07431_NG07431

description:
test hybridization
NG07431


title: NG07430_NG07430

description:
test hybridization
NG07430


title: NG07429_NG07429

description:
test hybridization
NG07429


title: NG07426_NG07426

description:
test hybridization
NG07426


title: NG07443_NG07443

description:
test hybridization
NG07443


title: NG07420_NG07420

description:
test hybridization
NG07420


title: NG07421_NG07421

description:
test hybridization
NG07421


title: NG07419_NG07419

description:
test hybridization
NG07419


title: NG07425_NG07425

description:
test hybridization
NG07425


title: NG07422_NG07422

description:
test hybridization
NG07422


title: NG07423_NG07423

description:
test hybridization
NG07423


title: NG07424_NG07424

description:
test hybridization
NG07424


title: NG07418_NG07418

description:
test hybridization
NG07418


title: NG07416_NG07416

description:
test hybridization
NG07416


title: NG07415_NG07415

description:
test hybridization
NG07415


title: NG07414_NG07414

description:
test hybridization
NG07414


title: NG07417_NG07417

description:
test hybridization
NG07417


title: NG07408_NG07408

description:
test hybridization
NG07408


title: NG07411_NG07411

description:
test hybridization
NG07411


title: NG07410_NG07410

description:
test hybridization
NG07410


title: NG07412_NG07412

description:
test hybridization
NG07412


title: NG07407_NG07407

description:
test hybridization
NG07407


title: NG07406_NG07406

description:
test hybridization
NG07406


title: NG07405_NG07405

description:
test hybridization
NG07405


title: NG07403_NG07403

description:
test hybridization
NG07403


title: NG07398_NG07398

description:
test hybridization
NG07398


title: NG07397_NG07397

description:
test hybridization
NG07397


title: NG07396_NG07396

description:
test hybridization
NG07396


title: NG07399_NG07399

description:
test hybridization
NG07399


title: NG07413_NG07413

description:
test hybridization
NG07413


title: NG07401_NG07401

description:
test hybridization
NG07401


title: NG07400_NG07400

description:
test hybridization
NG07400


title: NG07402_NG07402

description:
test hybridization
NG07402


title: NG07395_NG07395

description:
test hybridization
NG07395


title: NG07393_NG07393

description:
test hybridization
NG07393


title: NG07394_NG07394

description:
test hybridization
NG07394


title: NG07388_NG07388

description:
test hybridization
NG07388


title: NG07391_NG07391

description:
test hybridization
NG07391


title: NG07390_NG07390

description:
test hybridization
NG07390


title: NG07392_NG07392

description:
test hybridization
NG07392


title: NG07409_NG07409

description:
test hybridization
NG07409


title: NG07384_NG07384

description:
test hybridization
NG07384


title: NG07382_NG07382

description:
test hybridization
NG07382


title: NG07381_NG07381

description:
test hybridization
NG07381


title: NG07383_NG07383

description:
test hybridization
NG07383


title: NG07379_NG07379

description:
test hybridization
NG07379


title: NG07380_NG07380

description:
test hybridization
NG07380


title: NG07404_NG07404

description:
test hybridization
NG07404


title: NG07377_NG07377

description:
test hybridization
NG07377


title: NG07385_NG07385

description:
test hybridization
NG07385


title: NG07378_NG07378

description:
test hybridization
NG07378


title: NG07376_NG07376

description:
test hybridization
NG07376


title: NG07375_NG07375

description:
test hybridization
NG07375


title: NG07389_NG07389

description:
test hybridization
NG07389


title: NG07374_NG07374

description:
test hybridization
NG07374


title: NG07387_NG07387

description:
test hybridization
NG07387


title: NG07386_NG07386

description:
test hybridization
NG07386


title: NG07370_NG07370

description:
test hybridization
NG07370


title: NG07372_NG07372

description:
test hybridization
NG07372


title: NG07373_NG07373

description:
test hybridization
NG07373


title: NG07371_NG07371

description:
test hybridization
NG07371


title: NG07369_NG07369

description:
test hybridization
NG07369


title: NG07368_NG07368

description:
test hybridization
NG07368


title: NG07363_NG07363

description:
test hybridization
NG07363


title: NG07364_NG07364

description:
test hybridization
NG07364


title: NG07358_NG07358

description:
test hybridization
NG07358


title: NG07362_NG07362

description:
test hybridization
NG07362


title: NG07360_NG07360

description:
test hybridization
NG07360


title: NG07367_NG07367

description:
test hybridization
NG07367


title: NG07359_NG07359

description:
test hybridization
NG07359


title: NG07357_NG07357

description:
test hybridization
NG07357


title: NG07355_NG07355

description:
test hybridization
NG07355


title: NG07350_NG07350

description:
test hybridization
NG07350


title: NG07349_NG07349

description:
test hybridization
NG07349


title: NG07348_NG07348

description:
test hybridization
NG07348


title: NG07351_NG07351

description:
test hybridization
NG07351


title: NG07352_NG07352

description:
test hybridization
NG07352


title: NG07347_NG07347

description:
test hybridization
NG07347


title: NG07353_NG07353

description:
test hybridization
NG07353


title: NG07344_NG07344

description:
test hybridization
NG07344


title: NG07345_NG07345

description:
test hybridization
NG07345


title: NG07366_NG07366

description:
test hybridization
NG07366


title: NG07346_NG07346

description:
test hybridization
NG07346


title: NG07365_NG07365

description:
test hybridization
NG07365


title: NG07361_NG07361

description:
test hybridization
NG07361


title: NG07340_NG07340

description:
test hybridization
NG07340


title: NG07338_NG07338

description:
test hybridization
NG07338


title: NG07342_NG07342

description:
test hybridization
NG07342


title: NG07356_NG07356

description:
test hybridization
NG07356


title: NG07339_NG07339

description:
test hybridization
NG07339


title: NG07343_NG07343

description:
test hybridization
NG07343


title: NG07333_NG07333

description:
test hybridization
NG07333


title: NG07354_NG07354

description:
test hybridization
NG07354


title: NG07334_NG07334

description:
test hybridization
NG07334


title: NG07337_NG07337

description:
test hybridization
NG07337


title: NG07332_NG07332

description:
test hybridization
NG07332


title: NG07336_NG07336

description:
test hybridization
NG07336


title: NG07335_NG07335

description:
test hybridization
NG07335


title: NG07328_NG07328

description:
test hybridization
NG07328


title: NG07329_NG07329

description:
test hybridization
NG07329


title: NG07331_NG07331

description:
test hybridization
NG07331


title: NG07325_NG07325

description:
test hybridization
NG07325


title: NG07324_NG07324

description:
test hybridization
NG07324


title: NG07327_NG07327

description:
test hybridization
NG07327


title: NG07323_NG07323

description:
test hybridization
NG07323


title: NG07326_NG07326

description:
test hybridization
NG07326


title: NG07319_NG07319

description:
test hybridization
NG07319


title: NG07322_NG07322

description:
test hybridization
NG07322


title: NG07315_NG07315

description:
test hybridization
NG07315


title: NG07314_NG07314

description:
test hybridization
NG07314


title: NG07318_NG07318

description:
test hybridization
NG07318


title: NG07316_NG07316

description:
test hybridization
NG07316


title: NG07321_NG07321

description:
test hybridization
NG07321


title: NG07320_NG07320

description:
test hybridization
NG07320


title: NG07311_NG07311

description:
test hybridization
NG07311


title: NG07309_NG07309

description:
test hybridization
NG07309


title: NG07317_NG07317

description:
test hybridization
NG07317


title: NG07330_NG07330

description:
test hybridization
NG07330


title: NG07313_NG07313

description:
test hybridization
NG07313


title: NG07312_NG07312

description:
test hybridization
NG07312


title: NG07308_NG07308

description:
test hybridization
NG07308


title: NG07310_NG07310

description:
test hybridization
NG07310


title: NG07307_NG07307

description:
test hybridization
NG07307


title: NG07302_NG07302

description:
test hybridization
NG07302


title: NG07300_NG07300

description:
test hybridization
NG07300


title: NG07301_NG07301

description:
test hybridization
NG07301


title: NG07303_NG07303

description:
test hybridization
NG07303


title: NG07306_NG07306

description:
test hybridization
NG07306


title: NG07305_NG07305

description:
test hybridization
NG07305


title: NG07304_NG07304

description:
test hybridization
NG07304


title: NG07294_NG07294

description:
test hybridization
NG07294


title: NG07299_NG07299

description:
test hybridization
NG07299


title: NG07293_NG07293

description:
test hybridization
NG07293


title: NG07295_NG07295

description:
test hybridization
NG07295


title: NG07298_NG07298

description:
test hybridization
NG07298


title: NG07297_NG07297

description:
test hybridization
NG07297


title: NG07292_NG07292

description:
test hybridization
NG07292


title: NG07289_NG07289

description:
test hybridization
NG07289


title: NG07291_NG07291

description:
test hybridization
NG07291


title: NG07283_NG07283

description:
test hybridization
NG07283


title: NG07286_NG07286

description:
test hybridization
NG07286


title: NG07290_NG07290

description:
test hybridization
NG07290


title: NG07280_NG07280

description:
test hybridization
NG07280


title: NG07287_NG07287

description:
test hybridization
NG07287


title: NG07288_NG07288

description:
test hybridization
NG07288


title: NG07277_NG07277

description:
test hybridization
NG07277


title: NG07285_NG07285

description:
test hybridization
NG07285


title: NG07272_NG07272

description:
test hybridization
NG07272


title: NG07282_NG07282

description:
test hybridization
NG07282


title: NG07281_NG07281

description:
test hybridization
NG07281


title: NG07278_NG07278

description:
test hybridization
NG07278


title: NG07271_NG07271

description:
test hybridization
NG07271


title: NG07276_NG07276

description:
test hybridization
NG07276


title: NG07273_NG07273

description:
test hybridization
NG07273


title: NG07274_NG07274

description:
test hybridization
NG07274


title: NG07296_NG07296

description:
test hybridization
NG07296


title: NG07266_NG07266

description:
test hybridization
NG07266


title: NG07263_NG07263

description:
test hybridization
NG07263


title: NG07262_NG07262

description:
test hybridization
NG07262


title: NG07264_NG07264

description:
test hybridization
NG07264


title: NG07259_NG07259

description:
test hybridization
NG07259


title: NG07258_NG07258

description:
test hybridization
NG07258


title: NG07275_NG07275

description:
test hybridization
NG07275


title: NG07270_NG07270

description:
test hybridization
NG07270


title: NG07269_NG07269

description:
test hybridization
NG07269


title: NG07257_NG07257

description:
test hybridization
NG07257


title: NG07284_NG07284

description:
test hybridization
NG07284


title: NG07251_NG07251

description:
test hybridization
NG07251


title: NG07268_NG07268

description:
test hybridization
NG07268


title: NG07254_NG07254

description:
test hybridization
NG07254


title: NG07261_NG07261

description:
test hybridization
NG07261


title: NG07279_NG07279

description:
test hybridization
NG07279


title: NG07265_NG07265

description:
test hybridization
NG07265


title: NG07255_NG07255

description:
test hybridization
NG07255


title: NG07256_NG07256

description:
test hybridization
NG07256


title: NG07267_NG07267

description:
test hybridization
NG07267


title: NG07253_NG07253

description:
test hybridization
NG07253


title: NG07252_NG07252

description:
test hybridization
NG07252


title: NG07243_NG07243

description:
test hybridization
NG07243


title: NG07241_NG07241

description:
test hybridization
NG07241


title: NG07249_NG07249

description:
test hybridization
NG07249


title: NG07248_NG07248

description:
test hybridization
NG07248


title: NG07238_NG07238

description:
test hybridization
NG07238


title: NG07250_NG07250

description:
test hybridization
NG07250


title: NG07247_NG07247

description:
test hybridization
NG07247


title: NG07246_NG07246

description:
test hybridization
NG07246


title: NG07235_NG07235

description:
test hybridization
NG07235


title: NG07260_NG07260

description:
test hybridization
NG07260


title: NG07242_NG07242

description:
test hybridization
NG07242


title: NG07239_NG07239

description:
test hybridization
NG07239


title: NG07237_NG07237

description:
test hybridization
NG07237


title: NG07233_NG07233

description:
test hybridization
NG07233


title: NG07236_NG07236

description:
test hybridization
NG07236


title: NG07232_NG07232

description:
test hybridization
NG07232


title: NG07231_NG07231

description:
test hybridization
NG07231


title: NG07230_NG07230

description:
test hybridization
NG07230


title: NG07228_NG07228

description:
test hybridization
NG07228


title: NG07229_NG07229

description:
test hybridization
NG07229


title: NG07244_NG07244

description:
test hybridization
NG07244


title: NG07245_NG07245

description:
test hybridization
NG07245