title: SLAM Project Data: Synthetic Lethality with ylr281c

description:
Internal identifier for dataset: SL/100323/10
Original datafile name: 100323NG300342A02_532.pair
Original datafile2 name: 100323NG300342A02_635.pair
SD of values from reference genes: 0.413
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.90 1.75 2.09
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 226 well B12 (strain 25190)


title: SLAM Project Data: Synthetic Lethality with ylr280c

status: Public on Feb 28 2013

type: genomic

city: Baltimore

first: Jef

last: Boeke

address: 733 N BROADWAY # 339

country: USA

institute: Johns Hopkins U

description:
Internal identifier for dataset: SL/100323/9
Original datafile name: 100323NG300342A01_532.pair
Original datafile2 name: 100323NG300342A01_635.pair
SD of values from reference genes: 0.408
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.35 1.66 2.21
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

hyb_protocol: Labeled extracts were hybridized overnight to NimbleGen 72k 4-plex microarray slides at 42 degrees C.

scan_protocol: Images were acquired with a GenePix 4200AL scanner at 5 um resolution or an Agilent G2505C scanner at 2 um resolution

data_processing: Two-channel image files were analyzed with the NimbleScan software package (Roche NimbleGen, version 2.3.78 or higher). Annotations were reconstructed from data downloaded from the Saccharomyces Genome Database on 2008-11-15. Numerical data were obtained as the mean of the central pixels. Background signal intensities were not used. Custom R packages (hoptag v3.9-7; hoptagInfo07A v3.9-6) were used with their default options. See the software and PDF documentation included with this GEO Series submission for details. The VALUE column represents a dynamically weighted sum of data from the UpTag and DnTag probes for each gene.

molecule: genomic DNA

organism: Saccharomyces cerevisiae

source_name: Reference

label_protocol: UpTag and DnTag sequences were amplified from genomic DNA by asymmetric PCR using labeled primers.

growth_protocol: Cells were grown at 30 degrees C for approximately eight doublings using "Magic Marker" selectable medium supplemented with uracil.

extract_protocol: Genomic DNA was extracted by phenol extraction or by use of proprietary kits (e.g., Epicentre, Norgen).

tag: query gene

tag: knockout clone

value: Plate 226 well B11 (strain 25189)

tag: genetic background

tag: pool genotype

value: MATa/alpha ura3delta0 leu2delta his3delta1 lys2delta0/LYS2 met15delta0/MET15 can1delta::LEU2-MFA1pr-HIS3/CAN1 xxx::kanMX/XXX

source_name: Test

growth_protocol: Cells were grown without added uracil, using materials and procedures that were otherwise the same as those for Channel 1.


title: RNA pool 2, treated allergic group, polinization period

type: RNA

city: Madrid

first: Miriam

last: Aguerri

address: avd/Reyes Católicos, 2

department: Immunology

country: Spain

institute: IIS-Fundación Jiménez Díaz

description: GROUP 5 POOL 2 PRE

hyb_protocol: Following fragmentation, 10 µg of cDNA were hybridized for 16 hr at 45C on the GeneChip HuGe U133 plus 2.0 . GeneChips were washed and stained in the Affymetrix Fluidics Station 400.

scan_protocol: Microarrays were scanned using the GeneChip® Scanner 3000, enabled for High-Resolution Scanning

data_processing: Preliminary microarray analysis included assessment of data quality by standard quality checks and principal component assays. First-line check, background corrections, and quantile normalization of the data were performed by Robust Multiarray Averaging (RMA) method. Data analysis was performed using Partek Genomics Suite software. Affymetrix raw data files (CEL files) where background corrected using RMA methodology. Intensity signal was standardized across arrays via quantile normalization algorithm. Further analyses where carried out using Babelomics web suite.

label: Biotin

molecule: total RNA

organism: Homo sapiens

source_name: RNA from PBMCs of 2 treated allergic subjects during polinization

label_protocol: Microarray targets were prepared from 200 ng of total RNA using the Affymetrix (Santa Clara, CA, USA) kit for reverse transcription and a double round of in vitro transcription. Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 10 µg of cDNA.

growth_protocol: PBMCs isolated from peripheral blood by density gradient

extract_protocol: Total RNA was isolated from PBMCs (106 cells) using Trizol (Invitrogen, Carlsbad, CA, USA) extraction and isopropyl alcohol precipitation. RNA was quantified by spectrophotometry in a nanodrop, and integrity was confirmed with the Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA).We utilized an RNA-pooling strategy to maximize the number of individuals in the microarray study to increase statistical power and representation of intra-individual variability in gene expression.Three independent pools were created for each clinical group of study (non-allergic, asymptomatic, allergic non-related to olive pollen, allergic to olive pollen without treatment and allergic to olive pollen with specific treatment) and there were two different pollen exposition condition (during and outside pollen season). Total number of RNAs analyzed by microarrays was of 28 samples.

tag: period

value: Polinization period

tag: clinical group

value: Treated allergic group


title: SLAM Project Data: Synthetic Lethality with ylr218c

description:
Internal identifier for dataset: SL/090505/19
Original datafile name: 090505NG142907BA03_532.pair
Original datafile2 name: 090505NG142907BA03_635.pair
SD of values from reference genes: 0.389
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.14 2.64 2.86
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 220 well C12 (strain 24167)


title: SLAM Project Data: Synthetic Lethality with ylr224w

description:
Internal identifier for dataset: SL/090513/4
Original datafile name: 090513NG142993BA04_532.pair
Original datafile2 name: 090513NG142993BA04_635.pair
SD of values from reference genes: 0.286
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 3.09 3.78 3.79
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 220 well D06 (strain 24173)


title: Muscle_Young Women_Rep1

city: Ann Arbor

first: GORDON

last: PAUL

state: MI

address: 325 E Eisenhower

institute: University of Michigan

description: Gene expression data from young healthy human subject

hyb_protocol: Biotin-labeled cDNA was hybridized to Affymetrix Human Genome U133 Plus 2.0 arrays (Santa Clara, CA) according to manufacturer's instructions. Following hybridization, the probe arrays were washed and stained.

scan_protocol: The intensity of bound dye was measured with an argon laser confocal scanner (GeneArray Scanner, Agilent), and the stored images were analyzed using the GeneChip software Microarray Analysis Suite (MAS) 5.0 (Affymetrix, Santa Clara, CA).

data_processing: Raw intensity values of all samples were preprocessed and normalized by RMA using Bioconductor. Differentially expressed genes were tested by using an intensity-based Bayesian moderated t-statistic.

label: biotin

source_name: Young women resting biceps

label_protocol: Total RNA was used as a template for double-stranded cDNA synthesis (Superscript Double-Stranded cDNA synthesis kit, Invitrogen, Carlsbad, CA). Biotin-labeledcDNA was synthesized (EnzoBioarray High Yield RNA transcription Labeling Kit, Affymetrix).

extract_protocol: Total RNA was extracted from frozen tissue with a polytron homogenizer (Brinkman, Westbury, NY) and Trizol reagent (Invitrogen, Rockville, MD) and purified with an RNAse kit (Qiagen, Santa Clara, CA).

tag: gender

value: Female

tag: age group

value: Young


title: RNA pool 3, treated allergic group, polinization period

description: GROUP 5 POOL 3 PRE


title: RNA pool 2, non-treated allergic group, post-polinization period

description: GROUP 4 POOL 2 POST

source_name: RNA from PBMCs of 2 non-treated allergic subjects outside polinization

value: Post- polinization period

value: Untreated allergic group


title: RNA pool 3, non-treated allergic group, polinization period

description: GROUP 4 POOL 3 PRE

source_name: RNA from PBMCs of 2 non-treated allergic subjects during polinization


title: SLAM Project Data: Synthetic Lethality with ylr225c

description:
Internal identifier for dataset: SL/090513/5
Original datafile name: 090513NG142994BA01_532.pair
Original datafile2 name: 090513NG142994BA01_635.pair
SD of values from reference genes: 0.309
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.39 3.38 3.51
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 220 well D07 (strain 24174)


title: SLAM Project Data: Synthetic Lethality with ylr211c

description:
Internal identifier for dataset: SL/090505/13
Original datafile name: 090505NG142906BA01_532.pair
Original datafile2 name: 090505NG142906BA01_635.pair
SD of values from reference genes: 0.393
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.47 2.99 3.05
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate  well  (strain NA)


title: SLAM Project Data: Synthetic Lethality with ylr179c

description:
Internal identifier for dataset: SL/090402/10
Original datafile name: 090402NG142641A02_532.pair
Original datafile2 name: 090402NG142641A02_635.pair
SD of values from reference genes: 0.361
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.82 2.51 2.55
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 219 well H11 (strain 24128)


title: RNA pool 2, non-treated allergic group, polinization period

description: GROUP 4 POOL 2 PRE


title: RNA pool 1, non-treated allergic group, polinization period

description: GROUP 4 POOL 1 PRE


title: RNA pool 2, non-related to olive allergic group, post-polinization period

description: GROUP 3 POOL 2 POST

source_name: RNA from PBMCs of 2 non-related allergic subjects outside polinization

value: Non-related to olive allergic group


title: RNA pool 1, non-treated allergic group, post-polinization period

description: GROUP 4 POOL 1 POST


title: SLAM Project Data: Synthetic Lethality with ylr152c

description:
Internal identifier for dataset: SL/090331/22
Original datafile name: 090331NG142636A02_532.pair
Original datafile2 name: 090331NG142636A02_635.pair
SD of values from reference genes: 0.548
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.50 1.76 1.78
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 219 well G04 (strain 24109)


title: SLAM Project Data: Synthetic Lethality with ylr173w

description:
Internal identifier for dataset: SL/090402/7
Original datafile name: 090402NG142640BA03_532.pair
Original datafile2 name: 090402NG142640BA03_635.pair
SD of values from reference genes: 0.374
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.28 3.10 3.07
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 219 well H05 (strain 24122)


title: SLAM Project Data: Synthetic Lethality with ylr168c

description:
Internal identifier for dataset: SL/090402/1
Original datafile name: 090402NG142637A01_532.pair
Original datafile2 name: 090402NG142637A01_635.pair
SD of values from reference genes: 0.459
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.66 2.20 2.16
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 219 well G11 (strain 24117)


title: SLAM Project Data: Synthetic Lethality with ylr143w

description:
Internal identifier for dataset: SL/090331/19
Original datafile name: 090331NG142635BA03_532.pair
Original datafile2 name: 090331NG142635BA03_635.pair
SD of values from reference genes: 0.690
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.10 1.42 1.41
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 219 well F08 (strain 24100)


title: SLAM Project Data: Synthetic Lethality with ylr171w

description:
Internal identifier for dataset: SL/090402/5
Original datafile name: 090402NG142640BA01_532.pair
Original datafile2 name: 090402NG142640BA01_635.pair
SD of values from reference genes: 0.279
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.67 3.41 3.70
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 219 well H02 (strain 24120)

value: MATa/alpha ura3delta0 leu2delta his3delta1 lys2delta0/LYS2 met15delta0/MET15 can1delta::LEU2-MFA1pr-HIS3/CAN1 xxx::kanMX/XXX


title: SLAM Project Data: Synthetic Lethality with ylr169w

description:
Internal identifier for dataset: SL/090402/2
Original datafile name: 090402NG142637A02_532.pair
Original datafile2 name: 090402NG142637A02_635.pair
SD of values from reference genes: 0.362
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.98 2.90 2.99
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 219 well G12 (strain 24118)


title: SLAM Project Data: Synthetic Lethality with ylr149c

description:
Internal identifier for dataset: SL/090331/21
Original datafile name: 090331NG142636A01_532.pair
Original datafile2 name: 090331NG142636A01_635.pair
SD of values from reference genes: 0.418
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.17 2.72 2.62
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 219 well G01 (strain 24106)


title: RNA pool 1, non-related to olive allergic group, polinization period

description: GROUP 3 POOL 1 PRE

source_name: RNA from PBMCs of 2 non-related allergic subjects during polinization

value: Polinization period


title: SLAM Project Data: Synthetic Lethality with ylr126c

description:
Internal identifier for dataset: SL/090331/8
Original datafile name: 090331NG142631BA04_532.pair
Original datafile2 name: 090331NG142631BA04_635.pair
SD of values from reference genes: 0.338
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.50 2.87 2.99
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 219 well E03 (strain 24083)


title: RNA pool 3, asymptomatic group, post-polinization period

description: GROUP 2 POOL 3 POST

source_name: RNA from PBMCs of 2 asymptomatic subjects outside polinization

value: Asymptomatic group


title: RNA pool 1, non-related to olive allergic group, post-polinization period

description: GROUP 3 POOL 1 POST

value: Post- polinization period


title: RNA pool 2, asymptomatic group, polinization period

description: GROUP 2 POOL 2 PRE

source_name: RNA from PBMCs of 2 asymptomatic subjects during polinization


title: RNA pool 2, asymptomatic group, post-polinization period

description: GROUP 2 POOL 2 POST


title: RNA pool 3, asymptomatic group, polinization period

description: GROUP 2 POOL 3 PRE


title: RNA pool 1, asymptomatic group, post-polinization period

description: GROUP 2 POOL 1 POST


title: RNA pool 2, non-related to olive allergic group, polinization period

description: GROUP 3 POOL 2 PRE


title: SLAM Project Data: Synthetic Lethality with ylr041w

description:
Internal identifier for dataset: SL/100617/22
Original datafile name: 308390A02_532.pair
Original datafile2 name: 308390A02_635.pair
SD of values from reference genes: 0.350
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.44 2.21 2.53
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 240 well F01 (strain 26439)


title: SLAM Project Data: Synthetic Lethality with ylr031w

description:
Internal identifier for dataset: SL/100617/16
Original datafile name: 308388A04_532.pair
Original datafile2 name: 308388A04_635.pair
SD of values from reference genes: 0.325
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.76 2.65 2.96
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 240 well E03 (strain 26429)


title: RNA pool 3, non-allergic group, post-polinization period

description: GROUP 1 POOL 3 POST

source_name: RNA from PBMCs of 2 non-allergic subjects outside polinization

value: Non-allergic group


title: SLAM Project Data: Synthetic Lethality with ylr050c

description:
Internal identifier for dataset: SL/100617/23
Original datafile name: 308390A03_532.pair
Original datafile2 name: 308390A03_635.pair
SD of values from reference genes: 0.298
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 3.23 2.79 3.31
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 240 well F02 (strain 26440)


title: SLAM Project Data: Synthetic Lethality with yll059c

description:
Internal identifier for dataset: SL/100617/14
Original datafile name: 308388A02_532.pair
Original datafile2 name: 308388A02_635.pair
SD of values from reference genes: 0.320
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.85 2.38 2.74
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 240 well E01 (strain 26427)


title: SLAM Project Data: Synthetic Lethality with ylr040c

description:
Internal identifier for dataset: SL/100617/21
Original datafile name: 308390A01_532.pair
Original datafile2 name: 308390A01_635.pair
SD of values from reference genes: 0.384
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.19 1.78 2.11
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 240 well E12 (strain 26438)


title: SLAM Project Data: Synthetic Lethality with yll030c

description:
Internal identifier for dataset: SL/100617/13
Original datafile name: 308388A01_532.pair
Original datafile2 name: 308388A01_635.pair
SD of values from reference genes: 0.273
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 3.03 2.72 3.14
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 240 well D09 (strain 26423)


title: RNA pool 1, non-allergic group, post-polinization period

description: GROUP 1 POOL 1 POST


title: RNA pool 2, non-allergic group, post-polinization period

description: GROUP 1 POOL 2 POST


title: SLAM Project Data: Synthetic Lethality with ykr074w

description:
Internal identifier for dataset: SL/100518/19
Original datafile name: 308460A03_532.pair
Original datafile2 name: 308460A03_635.pair
SD of values from reference genes: 0.272
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.19 2.64 2.69
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 239 well A06 (strain 25990)


title: SLAM Project Data: Synthetic Lethality with ykr073c

description:
Internal identifier for dataset: SL/100518/18
Original datafile name: 308460A02_532.pair
Original datafile2 name: 308460A02_635.pair
SD of values from reference genes: 0.317
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.36 1.93 2.42
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 239 well A05 (strain 25989)


title: SLAM Project Data: Synthetic Lethality with ylr030w

description:
Internal identifier for dataset: SL/100617/15
Original datafile name: 308388A03_532.pair
Original datafile2 name: 308388A03_635.pair
SD of values from reference genes: 0.301
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 3.02 2.85 3.05
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 240 well E02 (strain 26428)


title: SLAM Project Data: Synthetic Lethality with ykr075c

description:
Internal identifier for dataset: SL/100518/20
Original datafile name: 308460A04_532.pair
Original datafile2 name: 308460A04_635.pair
SD of values from reference genes: 0.379
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.14 2.12 2.32
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 239 well A07 (strain 25991)

value: MATa/alpha ura3delta0 leu2delta his3delta1 lys2delta0/LYS2 met15delta0/MET15 can1delta::LEU2-MFA1pr-HIS3/CAN1 xxx::kanMX/XXX


title: RNA pool 3, non-allergic group, polinization period

description: GROUP 1 POOL 3 PRE

source_name: RNA from PBMCs of 2 non-allergic subjects during polinization


title: RNA pool 2, non-allergic group, polinization period

description: GROUP 1 POOL 2 PRE


title: RNA pool 1, asymptomatic group, polinization period

description: GROUP 2 POOL 1 PRE


title: RNA pool 1, non-allergic group, polinization period

description: GROUP 1 POOL 1 PRE


title: SLAM Project Data: Synthetic Lethality with ykr051w

description:
Internal identifier for dataset: SL/100525/17
Original datafile name: 308510A01_532.pair
Original datafile2 name: 308510A01_635.pair
SD of values from reference genes: 0.526
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.70 1.02 1.49
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 235 well H05 (strain 25122)


title: SLAM Project Data: Synthetic Lethality with ykr043c

description:
Internal identifier for dataset: SL/100505/18
Original datafile name: 308318A02_532.pair
Original datafile2 name: 308318A02_635.pair
SD of values from reference genes: 0.403
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.86 1.80 2.03
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 235 well G09 (strain 25114)


title: SLAM Project Data: Synthetic Lethality with ykr070w

description:
Internal identifier for dataset: SL/100518/16
Original datafile name: 308459A04_532.pair
Original datafile2 name: 308459A04_635.pair
SD of values from reference genes: 0.287
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.40 2.84 2.88
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 239 well A02 (strain 25986)


title: SLAM Project Data: Synthetic Lethality with ykr047w

description:
Internal identifier for dataset: SL/100505/21
Original datafile name: 308323A01_532.pair
Original datafile2 name: 308323A01_635.pair
SD of values from reference genes: 0.382
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.93 1.80 2.17
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 235 well G12 (strain 25118)


title: SLAM Project Data: Synthetic Lethality with ykr033c

description:
Internal identifier for dataset: SL/100505/15
Original datafile name: 308316A03_532.pair
Original datafile2 name: 308316A03_635.pair
SD of values from reference genes: 0.587
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.03 1.08 1.26
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 235 well G05 (strain 25104)


title: SLAM Project Data: Synthetic Lethality with ykr045c

description:
Internal identifier for dataset: SL/100505/20
Original datafile name: 308318A04_532.pair
Original datafile2 name: 308318A04_635.pair
SD of values from reference genes: 0.526
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.22 1.55 1.68
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 235 well G11 (strain 25116)


title: Sample 5 GBM cells at 24 hours post injection, second experiment

city: padova

first: silvia

last: bresolin

state: padova

address: via giustiniani 3

department: department of women's and children's health

country: Italy

institute: università di padova

description: Gene expression data from human GBM cells after 24 hours post injection into zebrafish (second experiment)

hyb_protocol: Following fragmentation, 12,5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.

scan_protocol: GeneChips were scanned using the Scan GC3000 7G

data_processing: MAS 5.0

source_name: human GBM cells after transplantation into zebrafish

label_protocol: Biotinylated cRNA were prepared according to the standard Affymetrix 3'IVT expression kit protocol from 250 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).

growth_protocol: cells are cultured in DMEM-F12 supplemented with BIT9500 BFGF and EGF

extract_protocol: Total RNA using Trizol

treatment_protocol: human GBM cells engrafted into zebrafish brain

tag: time

value: 24 hpi second exp

tag: cell type

value: human GBM cells

tag: microenvironment

value: zebrafish brain


title: Sample 2 GBM cells at 24 hours post injection, first experiment

description: Gene expression data from human GBM cells after 24 hours post injection into zebrafish (first experiment)

value: 24 hpi first exp


title: Macaque liver adult 3

city: Shanghai

first: Zhao

last: Guodong

address: Yueyang Road

country: China

institute: PICB

description: Gene expression of Macaque liver adult 3

hyb_protocol: Hybridization to Affymetrix® Human Exon 1.0 ST arrays was carried out following standard Affymetrix protocols.

scan_protocol: Affymetrix Gene ChIP Scanner 3000 7G

data_processing:
We firstly mapped probes in Human Exon 1.0 ST Chip to human genome (hg18), chimpanzee genome (panTro2.1) and rhesus macaque genome (MMUL1.0) separately by using Bowtie [36]. And then we masked out probes that could not match perfectly and uniquely any of the respective genome. To exclude those probes with signal intensities below background noise, we compared each probe’s intensity to the distribution of anti-genomic probes’ intensities with the same GC content (the latter are designed to detect non-specific hybridization). A “detectable” probe was defined as one with intensity larger than 95% percentile of the anti-genomic probes’ intensities (comparing only to those anti-genomic probes with the same GC content). Detectable probe intensity values were log2 transformed and quantile normalized. If a transcript had both more than 80% probes and more than 10 probes detected, we likewise called this a detectable transcript.
probe group file: HuEx-1_0-st-v2.r2.pgf
meta-probeset file: HuEx-1_0-st-v2.r2.all.ps

organism: Macaca mulatta

source_name: Dissected macaque post-mortem liver

label_protocol: Biotinylated cRNA were prepared from 2 microg. total RNA following standard Affymetrix protocols.

extract_protocol: Trizol extraction of total RNA from 100 mg of tissue was performed according to the manufacturer's instructions.

treatment_protocol: All human postmortem tissue samples were obtained from the NICHD Brain and Tissue Bank for Developmental Disorders (NICHDBB)(Baltimore, MD, USA). All subjects were defined as normal controls by forensic pathologists at the NICHDBB. No subjects with prolonged agonal state were used. Chimpanzee samples were obtained from the Yerkes Primate Center (Atlanta, GA, USA), from the Biomedical Primate Research Centre (Rijswijk, Netherlands) and from the Anthropological Institute of the University of Zurich (Switzerland).

tag: tissue

value: liver


title: SLAM Project Data: Synthetic Lethality with ykr032w

description:
Internal identifier for dataset: SL/100505/14
Original datafile name: 308316A02_532.pair
Original datafile2 name: 308316A02_635.pair
SD of values from reference genes: 0.301
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.63 2.02 2.58
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 235 well G04 (strain 25103)


title: Macaque liver adult 2

description: Gene expression of Macaque liver adult 2


title: Sample 4 GBM cells at 4 hours post injection, second experiment

description: Gene expression data from human GBM cells after 4 hours post injection into zebrafish (second experiment)

value: 4 hpi second exp


title: Sample 6 GBM cells at 48 hours post injection, second experiment

description: Gene expression data from human GBM cells after 48 hours post injection into zebrafish (second experiment)

value: 48 hpi second exp


title: SLAM Project Data: Synthetic Lethality with ykr017c

description:
Internal identifier for dataset: SL/090624/6
Original datafile name: 090624NG129649A02_532.pair
Original datafile2 name: 090624NG129649A02_635.pair
SD of values from reference genes: 0.598
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.62 1.73 1.79
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 235 well F06 (strain 25088)


title: Macaque liver adult 1

description: Gene expression of Macaque liver adult 1


title: SLAM Project Data: Synthetic Lethality with ykr018c

description:
Internal identifier for dataset: SL/100505/8
Original datafile name: 308308A04_532.pair
Original datafile2 name: 308308A04_635.pair
SD of values from reference genes: 0.386
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.03 1.79 2.11
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 235 well F07 (strain 25089)


title: Chimpanzee liver adult 5

description: Gene expression of Chimpanzee liver adult 5

organism: Pan troglodytes

source_name: Dissected chimpanzee post-mortem liver


title: RNA pool 1, treated allergic group, polinization period

description: GROUP 5 POOL 1 PRE

value: Polinization period

value: Treated allergic group


title: Chimpanzee liver adult 6

description: Gene expression of Chimpanzee liver adult 6


title: Macaque liver adult 4

description: Gene expression of Macaque liver adult 4


title: SLAM Project Data: Synthetic Lethality with ykr016w

description:
Internal identifier for dataset: SL/100505/7
Original datafile name: 308308A03_532.pair
Original datafile2 name: 308308A03_635.pair
SD of values from reference genes: 0.446
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.93 1.40 1.88
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 235 well F05 (strain 25087)

value: MATa/alpha ura3delta0 leu2delta his3delta1 lys2delta0/LYS2 met15delta0/MET15 can1delta::LEU2-MFA1pr-HIS3/CAN1 xxx::kanMX/XXX


title: SLAM Project Data: Synthetic Lethality with ykr015c

description:
Internal identifier for dataset: SL/100505/6
Original datafile name: 308308A02_532.pair
Original datafile2 name: 308308A02_635.pair
SD of values from reference genes: 0.410
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.18 1.61 2.10
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 235 well F04 (strain 25086)


title: Chimpanzee liver adult 4

description: Gene expression of Chimpanzee liver adult 4


title: Chimpanzee liver adult 3

description: Gene expression of Chimpanzee liver adult 3


title: Sample 1 GBM cells at 4 hours post injection, first experiment

description: Gene expression data from human GBM cells after 4 hours post injection into zebrafish (first experiment)

value: 4 hpi first exp


title: Sample 3 GBM cells at 48 hours post injection, first experiment

description: Gene expression data from human GBM cells after 48 hours post injection into zebrafish (first experiment)

value: 48 hpi first exp


title: SLAM Project Data: Synthetic Lethality with ykl207w

description:
Internal identifier for dataset: SL/100428/23
Original datafile name: 308152A03_532.pair
Original datafile2 name: 308152A03_635.pair
SD of values from reference genes: 0.244
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.07 3.14 3.09
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 235 well D03 (strain 25057)


title: SLAM Project Data: Synthetic Lethality with ykl187c

description:
Internal identifier for dataset: SL/090624/23
Original datafile name: 090624NG129653A03_532.pair
Original datafile2 name: 090624NG129653A03_635.pair
SD of values from reference genes: 0.457
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.06 2.12 2.30
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 221 well D03 (strain 25037)


title: Chimpanzee liver adult 2

description: Gene expression of Chimpanzee liver adult 2


title: Human liver adult 5

description: Gene expression of Human liver adult 5

source_name: Dissected human post-mortem liver


title: Chimpanzee liver adult 1

description: Gene expression of Chimpanzee liver adult 1


title: SLAM Project Data: Synthetic Lethality with ykl171w

description:
Internal identifier for dataset: SL/090811/23
Original datafile name: 090811NG264515A03_532.pair
Original datafile2 name: 090811NG264515A03_635.pair
SD of values from reference genes: 0.372
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.20 2.94 2.65
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 221 well B11 (strain 25021)


title: Human liver adult 6

description: Gene expression of Human liver adult 6


title: SLAM Project Data: Synthetic Lethality with ykl177w

description:
Internal identifier for dataset: SL/090814/2
Original datafile name: 090814NG264519A02_532.pair
Original datafile2 name: 090814NG264519A02_635.pair
SD of values from reference genes: 0.397
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.27 2.29 2.43
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 221 well C05 (strain 25027)


title: SLAM Project Data: Synthetic Lethality with ykl147c

description:
Internal identifier for dataset: SL/091103/11
Original datafile name: 091103NG300076BA03_532.pair
Original datafile2 name: 091103NG300076BA03_635.pair
SD of values from reference genes: 0.278
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.06 3.25 3.20
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 222 well H06 (strain 24997)


title: SLAM Project Data: Synthetic Lethality with ykl162c-a

description:
Internal identifier for dataset: SL/100518/12
Original datafile name: 308455A04_532.pair
Original datafile2 name: 308455A04_635.pair
SD of values from reference genes: 0.261
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.21 3.37 3.18
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 238 well H08 (strain 25980)


title: SLAM Project Data: Synthetic Lethality with ykl161c

description:
Internal identifier for dataset: SL/090811/16
Original datafile name: 090811NG264513A04_532.pair
Original datafile2 name: 090811NG264513A04_635.pair
SD of values from reference genes: 0.311
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.43 2.70 3.05
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 221 well A12 (strain 25011)


title: Chimpanzee heart adult 5

description: Gene expression of Chimpanzee heart adult 5

source_name: Dissected chimpanzee post-mortem heart

value: heart


title: Chimpanzee heart adult 1

description: Gene expression of Chimpanzee heart adult 1


title: Chimpanzee heart adult 4

description: Gene expression of Chimpanzee heart adult 4


title: Human liver adult 1

description: Gene expression of Human liver adult 1

value: liver


title: Chimpanzee heart adult 2

description: Gene expression of Chimpanzee heart adult 2


title: Human liver adult 4

description: Gene expression of Human liver adult 4


title: SLAM Project Data: Synthetic Lethality with ykl105c

description:
Internal identifier for dataset: SL/090901/11
Original datafile name: 090901NG301356A03_532.pair
Original datafile2 name: 090901NG301356A03_635.pair
SD of values from reference genes: 0.428
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.02 1.58 1.93
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

source_name: Reference

value: Plate 222 well D12 (strain 24955)


title: SLAM Project Data: Synthetic Lethality with ykl131w

description:
Internal identifier for dataset: SL/091103/6
Original datafile name: 091103NG300069A02_532.pair
Original datafile2 name: 091103NG300069A02_635.pair
SD of values from reference genes: 0.404
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.43 2.71 2.84
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 222 well G01 (strain 24981)

source_name: Test


title: SLAM Project Data: Synthetic Lethality with ykl162c

description:
Internal identifier for dataset: SL/090811/17
Original datafile name: 090811NG264514A01_532.pair
Original datafile2 name: 090811NG264514A01_635.pair
SD of values from reference genes: 0.341
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.86 2.41 2.44
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 221 well B01 (strain 25012)


title: Human liver adult 3

description: Gene expression of Human liver adult 3


title: Chimpanzee heart adult 3

description: Gene expression of Chimpanzee heart adult 3


title: Human heart adult 5

description: Gene expression of Human heart adult 5

source_name: Dissected human post-mortem heart


title: SLAM Project Data: Synthetic Lethality with ykl133c

description:
Internal identifier for dataset: SL/090901/19
Original datafile name: 090901NG301369A03_532.pair
Original datafile2 name: 090901NG301369A03_635.pair
SD of values from reference genes: 0.339
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.37 2.20 2.59
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 222 well G03 (strain 24983)


title: SLAM Project Data: Synthetic Lethality with ykl136w

description:
Internal identifier for dataset: SL/090901/20
Original datafile name: 090901NG301369A04_532.pair
Original datafile2 name: 090901NG301369A04_635.pair
SD of values from reference genes: 0.257
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.80 3.48 3.38
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 222 well G06 (strain 24986)

value: MATa/alpha ura3delta0 leu2delta his3delta1 lys2delta0/LYS2 met15delta0/MET15 can1delta::LEU2-MFA1pr-HIS3/CAN1 xxx::kanMX/XXX


title: Human liver adult 2

description: Gene expression of Human liver adult 2


title: Human heart adult 3

description: Gene expression of Human heart adult 3


title: SLAM Project Data: Synthetic Lethality with ykl100c

description:
Internal identifier for dataset: SL/090901/7
Original datafile name: 090901NG301334A03_532.pair
Original datafile2 name: 090901NG301334A03_635.pair
SD of values from reference genes: 0.389
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.01 2.48 2.40
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 222 well D07 (strain 24950)


title: SLAM Project Data: Synthetic Lethality with ykl102c

description:
Internal identifier for dataset: SL/090901/9
Original datafile name: 090901NG301356A01_532.pair
Original datafile2 name: 090901NG301356A01_635.pair
SD of values from reference genes: 0.328
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.50 2.97 2.81
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 222 well D09 (strain 24952)


title: SLAM Project Data: Synthetic Lethality with ylr279w

description:
Internal identifier for dataset: SL/100323/8
Original datafile name: 100323NG300341A04_532.pair
Original datafile2 name: 100323NG300341A04_635.pair
SD of values from reference genes: 0.326
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.35 2.08 2.34
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 226 well B10 (strain 25188)


title: Human heart adult 4

description: Gene expression of Human heart adult 4


title: SLAM Project Data: Synthetic Lethality with ykl097c

description:
Internal identifier for dataset: SL/091022/6
Original datafile name: 091022NG299997BA02_532.pair
Original datafile2 name: 091022NG299997BA02_635.pair
SD of values from reference genes: 0.336
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.98 2.87 3.14
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 222 well D04 (strain 24946)


title: SLAM Project Data: Synthetic Lethality with ykl091c

description:
Internal identifier for dataset: SL/091009/2
Original datafile name: 091009NG299988A02_532.pair
Original datafile2 name: 091009NG299988A02_635.pair
SD of values from reference genes: 0.343
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.75 2.71 3.01
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 222 well C10 (strain 24940)


title: SLAM Project Data: Synthetic Lethality with ykl091c-rep2

description:
Internal identifier for dataset: SL/090901/1
Original datafile name: 090901NG300521A01_532.pair
Original datafile2 name: 090901NG300521A01_635.pair
SD of values from reference genes: 0.339
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.63 2.47 2.75
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).


title: Human heart adult 2

description: Gene expression of Human heart adult 2


title: SLAM Project Data: Synthetic Lethality with ykl077w

description:
Internal identifier for dataset: SL/090814/18
Original datafile name: 090814NG300514A02_532.pair
Original datafile2 name: 090814NG300514A02_635.pair
SD of values from reference genes: 0.292
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.76 2.21 2.65
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 222 well B08 (strain 24926)


title: SLAM Project Data: Synthetic Lethality with ykl075c

description:
Internal identifier for dataset: SL/090814/16
Original datafile name: 090814NG300509A04_532.pair
Original datafile2 name: 090814NG300509A04_635.pair
SD of values from reference genes: 0.522
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.67 1.81 1.97
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 222 well B06 (strain 24924)


title: Chimpanzee cortex adult 5

description: Gene expression of Chimpanzee cortex adult 5

source_name: Dissected chimpanzee post-mortem superior frontal gyrus

value: superior frontal gyrus of the brain


title: Chimpanzee cortex adult 3

description: Gene expression of Chimpanzee cortex adult 3


title: Chimpanzee cortex adult 4

description: Gene expression of Chimpanzee cortex adult 4


title: SLAM Project Data: Synthetic Lethality with ykl097c-rep2

description:
Internal identifier for dataset: SL/090921/1
Original datafile name: 090917NG301511A01_532.pair
Original datafile2 name: 090917NG301511A01_635.pair
SD of values from reference genes: 0.297
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.66 2.58 2.92
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).


title: SLAM Project Data: Synthetic Lethality with ykl071w

description:
Internal identifier for dataset: SL/090814/13
Original datafile name: 090814NG300509A01_532.pair
Original datafile2 name: 090814NG300509A01_635.pair
SD of values from reference genes: 0.360
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.18 2.18 2.37
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 222 well B02 (strain 24920)


title: SLAM Project Data: Synthetic Lethality with ykl070w

description:
Internal identifier for dataset: SL/090814/12
Original datafile name: 090814NG300366A04_532.pair
Original datafile2 name: 090814NG300366A04_635.pair
SD of values from reference genes: 0.409
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.77 1.85 1.88
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 222 well B01 (strain 24919)


title: Chimpanzee cortex adult 2

description: Gene expression of Chimpanzee cortex adult 2


title: Human cortex adult 5

description: Gene expression of Human cortex adult 5

source_name: Dissected human post-mortem superior frontal gyrus


title: Human cortex adult 4

description: Gene expression of Human cortex adult 4


title: Chimpanzee cortex adult 1

description: Gene expression of Chimpanzee cortex adult 1


title: SLAM Project Data: Synthetic Lethality with ykl061w

description:
Internal identifier for dataset: SL/090814/4
Original datafile name: 090814NG264519A04_532.pair
Original datafile2 name: 090814NG264519A04_635.pair
SD of values from reference genes: 0.425
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.56 2.35 2.51
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 222 well A04 (strain 24910)


title: SLAM Project Data: Synthetic Lethality with ykl063c

description:
Internal identifier for dataset: SL/090814/6
Original datafile name: 090814NG300040A02_532.pair
Original datafile2 name: 090814NG300040A02_635.pair
SD of values from reference genes: 0.295
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.97 2.81 3.11
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 222 well A06 (strain 24912)


title: SLAM Project Data: Synthetic Lethality with ykl053w

description:
Internal identifier for dataset: SL/090624/24
Original datafile name: 090624NG129653A04_532.pair
Original datafile2 name: 090624NG129653A04_635.pair
SD of values from reference genes: 0.372
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.22 2.53 2.86
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 220 well H12 (strain 24902)


title: Human cortex adult 2

description: Gene expression of Human cortex adult 2


title: SLAM Project Data: Synthetic Lethality with ykl050c

description:
Internal identifier for dataset: SL/090527/14
Original datafile name: 090527NG142880A02_532.pair
Original datafile2 name: 090527NG142880A02_635.pair
SD of values from reference genes: 0.377
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.52 2.57 2.85
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 220 well H09 (strain 24899)


title: Human cortex adult 3

description: Gene expression of Human cortex adult 3


title: SLAM Project Data: Synthetic Lethality with ykl066w

description:
Internal identifier for dataset: SL/090814/9
Original datafile name: 090814NG300366A01_532.pair
Original datafile2 name: 090814NG300366A01_635.pair
SD of values from reference genes: 0.286
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.49 2.62 2.68
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 222 well A09 (strain 24915)


title: SLAM Project Data: Synthetic Lethality with yjl215c

description:
Internal identifier for dataset: SL/100305/21
Original datafile name: 100305NG300380A01_532.pair
Original datafile2 name: 100305NG300380A01_635.pair
SD of values from reference genes: 0.511
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.98 1.61 1.99
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 225 well A02 (strain 21210)


title: Human cortex adult 1

description: Gene expression of Human cortex adult 1


title: SLAM Project Data: Synthetic Lethality with ykl023w

description:
Internal identifier for dataset: SL/090513/12
Original datafile name: 090513NG129635BA04_532.pair
Original datafile2 name: 090513NG129635BA04_635.pair
SD of values from reference genes: 0.435
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.31 3.08 2.79
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 220 well F06 (strain 24872)

value: MATa/alpha ura3delta0 leu2delta his3delta1 lys2delta0/LYS2 met15delta0/MET15 can1delta::LEU2-MFA1pr-HIS3/CAN1 xxx::kanMX/XXX


title: SLAM Project Data: Synthetic Lethality with yjl193w

description:
Internal identifier for dataset: SL/100316/6
Original datafile name: 100316NG300223A02_532.pair
Original datafile2 name: 100316NG300223A02_635.pair
SD of values from reference genes: 0.426
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.42 1.86 2.08
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 225 well B08 (strain 21232)


title: SLAM Project Data: Synthetic Lethality with yjl185c

description:
Internal identifier for dataset: SL/100316/11
Original datafile name: 100316NG300224A03_532.pair
Original datafile2 name: 100316NG300224A03_635.pair
SD of values from reference genes: 0.316
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.01 2.13 2.30
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 225 well C03 (strain 21240)


title: SLAM Project Data: Synthetic Lethality with yju3

description:
Internal identifier for dataset: SL/090901/5
Original datafile name: 090901NG301334A01_532.pair
Original datafile2 name: 090901NG301334A01_635.pair
SD of values from reference genes: 0.251
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.73 3.21 3.20
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 222 well D01 (strain 24943)


title: SLAM Project Data: Synthetic Lethality with ykl033w-a

description:
Internal identifier for dataset: SL/100604/24
Original datafile name: 308127A04_532.pair
Original datafile2 name: 308127A04_635.pair
SD of values from reference genes: 0.411
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.74 1.78 1.92
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate  well  (strain NA)


title: KO_right hippocampi_TFC_rep1

city: Hualien

first: Ingrid

last: Liu

address: E411, 701, Section 3 Chung Yang Road

department: Molecular Biology and Human Genetics

country: Taiwan

institute: Tzu Chi University

description:
S4_1
Sacrificed immediately after retrieval contextual memory.

hyb_protocol: Phalanx Mouse One Array (MOA) v1.1 arrays were pre-heated at 60℃ for 10 mins, and rehydrated by 100% ethanol followed by deionized water. The slides were pre-hybridized with 5x SSPE, 0.1% SDS and 1% BSA at 42℃ for 2 hours. After the pre-hybridization, 5 µg Cy5-labeled aRNA was hybridized on MOA in duplicate in Phalanx OneArray hybridization buffer.

scan_protocol: The arrays were scanned by an Axon GenePix 4000B scanner (635nm power 100 PMT 550~570 ; 532nm power 10, PMT 460), and the fluoresence intensities were quantified.

data_processing:
The raw intensity was filtered (SNR>5), and normalized by the quantile normalization method using Matlab.
The average ratios of the hybridization replicates were calculated and the significant values for each gene were found by pairwise comparision (Ranking and hypothesis testing).

organism: Mus musculus

source_name: right hippocampus, Cav3.2 KO, TFC

label_protocol: 2.5 µg of total RNA was reverse-transcribed and amplified using the MessageAmp aRNA Amplification Kit (Ambion).  Indirect labeling the aa-UTP whereon by NHS-CyDye (Cy5, Amersham).

growth_protocol: Animals were housed individually in plastic and metal cages with ad libitum access to food and water under a constant 12hr light/dark cycle.

extract_protocol: Total RNA extraction by Trizol. RNA purity was checked by optical density of NanoDrop ND-1000 and agarose electrophoresis with the result of A260/A280≧1.8, no gDNA contamination. RNA integrity was measured by Agilent RNA 6000 Nano Assay (RIN≧8).

treatment_protocol: All mice were trained and tested between 6 and 8 weeks old. WT and Cav3.2 KO mice were divided into naïve and TFC groups with 3 animals in each group. After contextual fear conditioning, all groups were sacrificed immediately and bilateral hippocampi were dissected.

tag: background/strain

tag: genotype

value: Cav3.2 knockout

value: right hippocampus

tag: age

value: 6-8 weeks

tag: Sex

value: male

tag: treatment

value: TFC


title: KO_right hippocampi_naive_rep1

source_name: right hippocampus, Cav3.2 KO, naive


title: SLAM Project Data: Synthetic Lethality with yjl160c

description:
Internal identifier for dataset: SL/100316/18
Original datafile name: 100316NG300238A02_532.pair
Original datafile2 name: 100316NG300238A02_635.pair
SD of values from reference genes: 0.333
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.89 2.54 2.96
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

tag: query gene


title: SLAM Project Data: Synthetic Lethality with yjl169w

description:
Internal identifier for dataset: SL/100316/16
Original datafile name: 100316NG300227A04_532.pair
Original datafile2 name: 100316NG300227A04_635.pair
SD of values from reference genes: 0.484
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.57 1.21 1.56
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

tag: knockout clone

value: Plate 225 well D03 (strain 21256)


title: KO_right hippocampi_naive_rep2


title: SLAM Project Data: Synthetic Lethality with yjl181w

description:
Internal identifier for dataset: SL/100316/12
Original datafile name: 100316NG300224A04_532.pair
Original datafile2 name: 100316NG300224A04_635.pair
SD of values from reference genes: 0.430
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.94 1.45 1.84
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 225 well C06 (strain 21243)

tag: genetic background


title: SLAM Project Data: Synthetic Lethality with yjl144w

description:
Internal identifier for dataset: SL/100317/10
Original datafile name: 100317NG300296A02_532.pair
Original datafile2 name: 100317NG300296A02_635.pair
SD of values from reference genes: 0.309
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.73 2.12 2.85
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 225 well E11 (strain 21281)

tag: pool genotype


title: KO_right hippocampi_TFC_rep2

description:
S4_2
Sacrificed immediately after retrieval contextual memory.


title: WT_right hippocampi_TFC_rep1

description:
S2_1
Sacrificed immediately after retrieval contextual memory.

source_name: right hippocampus, WT, TFC

value: wild type


title: WT_right hippocampi_naive_rep2

source_name: right hippocampus, WT, naive


title: WT_right hippocampi_naive_rep1


title: SLAM Project Data: Synthetic Lethality with yjl149w

description:
Internal identifier for dataset: SL/090624/14
Original datafile name: 090624NG129651A02_532.pair
Original datafile2 name: 090624NG129651A02_635.pair
SD of values from reference genes: 0.532
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.74 1.88 2.16
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 225 well E06 (strain 21276)


title: KO_left hippocampi_TFC_rep2

description:
S3_2
Sacrificed immediately after retrieval contextual memory.

source_name: left hippocampus, Cav3.2 KO, TFC

value: left hippocampus


title: SLAM Project Data: Synthetic Lethality with yjl152w

description:
Internal identifier for dataset: SL/100317/7
Original datafile name: 100317NG300280A03_532.pair
Original datafile2 name: 100317NG300280A03_635.pair
SD of values from reference genes: 0.341
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.71 2.46 2.83
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 225 well E04 (strain 21273)


title: WT_right hippocampi_TFC_rep2

description:
S2_2
Sacrificed immediately after retrieval contextual memory.


title: SLAM Project Data: Synthetic Lethality with ykl044w

description:
Internal identifier for dataset: SL/090513/24
Original datafile name: 090513NG129638BA04_532.pair
Original datafile2 name: 090513NG129638BA04_635.pair
SD of values from reference genes: 0.393
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.47 3.02 3.09
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 220 well H02 (strain 24893)

value: MATa/alpha ura3delta0 leu2delta his3delta1 lys2delta0/LYS2 met15delta0/MET15 can1delta::LEU2-MFA1pr-HIS3/CAN1 xxx::kanMX/XXX


title: SLAM Project Data: Synthetic Lethality with yjl131c

description:
Internal identifier for dataset: SL/100317/17
Original datafile name: 100317NG300310A01_532.pair
Original datafile2 name: 100317NG300310A01_635.pair
SD of values from reference genes: 0.483
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.68 1.36 1.64
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 225 well F07 (strain 21294)


title: SLAM Project Data: Synthetic Lethality with yjl132w

description:
Internal identifier for dataset: SL/100317/16
Original datafile name: 100317NG300298A04_532.pair
Original datafile2 name: 100317NG300298A04_635.pair
SD of values from reference genes: 0.596
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.26 1.28 1.36
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).


title: SLAM Project Data: Synthetic Lethality with yir024c

description:
Internal identifier for dataset: SL/100616/15
Original datafile name: 308141A03_532.pair
Original datafile2 name: 308141A03_635.pair
SD of values from reference genes: 0.295
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.65 2.75 2.91
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 238 well G03 (strain 25959)


title: KO_left hippocampi_TFC_rep1

description:
S3_1
Sacrificed immediately after retrieval contextual memory.


title: SLAM Project Data: Synthetic Lethality with yir035c

description:
Internal identifier for dataset: SL/100603/5
Original datafile name: 310200A01_532.pair
Original datafile2 name: 310200A01_635.pair
SD of values from reference genes: 0.346
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.56 1.97 2.53
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 238 well G12 (strain 25970)


title: WT_left hippocampi_TFC_rep2

description:
S1_2
Sacrificed immediately after retrieval contextual memory.

source_name: left hippocampus, WT, TFC


title: WT_left hippocampi_naive_rep2

source_name: left hippocampus, WT, naive


title: KO_left hippocampi_naive_rep1

source_name: left hippocampus, Cav3.2 KO, naive

value: Cav3.2 knockout


title: KO_left hippocampi_naive_rep2


title: SLAM Project Data: Synthetic Lethality with yir020c

description:
Internal identifier for dataset: SL/100616/13
Original datafile name: 308141A01_532.pair
Original datafile2 name: 308141A01_635.pair
SD of values from reference genes: 0.406
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.08 1.90 2.20
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 238 well F11 (strain 25954)


title: WT_left hippocampi_naive_rep1

value: 6-8 weeks


title: SLAM Project Data: Synthetic Lethality with yip4

description:
Internal identifier for dataset: SL/100407/4
Original datafile name: 308419A04_532.pair
Original datafile2 name: 308419A04_635.pair
SD of values from reference genes: 0.448
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.69 1.76 2.07
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 228 well G09 (strain 24565)


title: SLAM Project Data: Synthetic Lethality with yil096c

description:
Internal identifier for dataset: SL/100525/11
Original datafile name: 308504A03_532.pair
Original datafile2 name: 308504A03_635.pair
SD of values from reference genes: 0.492
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): NA 1.24 1.27
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 237 well E11 (strain 21487)


title: SLAM Project Data: Synthetic Lethality with yil086c

description:
Internal identifier for dataset: SL/100525/7
Original datafile name: 308501A03_532.pair
Original datafile2 name: 308501A03_635.pair
SD of values from reference genes: 0.362
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.87 2.10 2.24
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 237 well E05 (strain 21477)


title: SLAM Project Data: Synthetic Lethality with yil064w

description:
Internal identifier for dataset: SL/100518/9
Original datafile name: 308455A01_532.pair
Original datafile2 name: 308455A01_635.pair
SD of values from reference genes: 0.289
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.30 2.07 2.62
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 237 well D07 (strain 21457)


title: SLAM Project Data: Synthetic Lethality with yil087c

description:
Internal identifier for dataset: SL/100525/8
Original datafile name: 308501A04_532.pair
Original datafile2 name: 308501A04_635.pair
SD of values from reference genes: 0.441
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.69 1.12 1.55
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 237 well E06 (strain 21478)


title: SLAM Project Data: Synthetic Lethality with glo1

description:
Internal identifier for dataset: SL/050527/5
Original datafile name: 050527 204_C01.gpr
Original datafile2 name: <none>
SD of values from reference genes: 0.850
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 0.92 1.28 1.22
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

hyb_protocol: Labeled extracts were hybridized overnight to Agilent 22k microarray slides at 42 degrees C.

scan_protocol: Images were acquired with a GenePix 4000B or 4200 AL scanner at 10 um resolution.

data_processing: Two-channel image files were analyzed with the GenePix Pro software package (Molecular Dynamics, version 5.1 or higher). Annotations were reconstructed from data downloaded from the Saccharomyces Genome Database on 2008-11-15. Numerical data were obtained as the median of foreground signal intensities. Background signal intensities were not used. Custom R packages (hoptag v3.9-7; hoptagInfo02A v3.9-6) were used with their default options. See the software and PDF documentation included with this GEO Series submission for details. The VALUE column represents a dynamically weighted sum of data from the UpTag and DnTag probes for each gene.

value: Plate 204 well C01 (strain 20572)


title: SLAM Project Data: Synthetic Lethality with gis1

description:
Internal identifier for dataset: SL/051019/6
Original datafile name: 051019_212_H08.gpr
Original datafile2 name: <none>
SD of values from reference genes: 0.775
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 0.90 1.50 1.55
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 212 well H08 (strain 24031)


title: SLAM Project Data: Synthetic Lethality with gis4

description:
Internal identifier for dataset: SL/050527/3
Original datafile name: 050527 204_B11.gpr
Original datafile2 name: <none>
SD of values from reference genes: 0.551
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.26 2.16 2.00
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 204 well B11 (strain 20570)


title: SLAM Project Data: Synthetic Lethality with gip4

description:
Internal identifier for dataset: SL/040219/2
Original datafile name: 201C10a_1316.gpr
Original datafile2 name: <none>
SD of values from reference genes: 0.544
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): NA 1.37 1.36
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 201 well C10 (strain 20377)


title: SLAM Project Data: Synthetic Lethality with yhr202w

description:
Internal identifier for dataset: SL/090226/7
Original datafile name: 090226NG142347A03_532.pair
Original datafile2 name: 090226NG142347A03_635.pair
SD of values from reference genes: 0.450
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.60 1.44 1.60
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 218 well F02 (strain 22896)


title: SLAM Project Data: Synthetic Lethality with gip4-rep2

description:
Internal identifier for dataset: SL/051027/3
Original datafile name: 051027_201_C10.gpr
Original datafile2 name: <none>
SD of values from reference genes: 0.344
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.87 2.94 3.04
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: MATa/alpha ura3delta0 leu2delta his3delta1 lys2delta0/LYS2 met15delta0/MET15 can1delta::LEU2-MFA1pr-HIS3/CAN1 xxx::kanMX/XXX


title: SLAM Project Data: Synthetic Lethality with yhr199c

description:
Internal identifier for dataset: SL/090226/5
Original datafile name: 090226NG142347A01_532.pair
Original datafile2 name: 090226NG142347A01_635.pair
SD of values from reference genes: 0.288
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.79 1.91 1.94
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 218 well E12 (strain 22893)


title: SLAM Project Data: Synthetic Lethality with gim4

description:
Internal identifier for dataset: SL/050729/3.1
Original datafile name: 050729_214_D07_OS.gpr
Original datafile2 name: <none>
SD of values from reference genes: 0.430
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.90 1.80 2.07
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 214 well D07 (strain 20243)


title: SLAM Project Data: Synthetic Lethality with glo4

description:
Internal identifier for dataset: SL/050504/7
Original datafile name: 050504_207_F07.gpr
Original datafile2 name: <none>
SD of values from reference genes: 0.601
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 0.29 1.39 1.36
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 207 well F07 (strain 21816)


title: SLAM Project Data: Synthetic Lethality with yil001w

description:
Internal identifier for dataset: SL/090624/12
Original datafile name: 090624NG129650A04_532.pair
Original datafile2 name: 090624NG129650A04_635.pair
SD of values from reference genes: 0.329
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 2.96 2.32 3.21
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 237 well A03 (strain 21393)


title: SLAM Project Data: Synthetic Lethality with gim5-rep2

description:
Internal identifier for dataset: SL/050817/4
Original datafile name: 050817_241_B01_OS.gpr
Original datafile2 name: <none>
SD of values from reference genes: 0.479
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.71 0.92 1.77
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 241 well B01 (strain 26491)


title: SLAM Project Data: Synthetic Lethality with gga1

description:
Internal identifier for dataset: SL/051101/8
Original datafile name: 051101_213_F04.gpr
Original datafile2 name: <none>
SD of values from reference genes: 0.522
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.07 1.75 1.86
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 213 well F04 (strain 24195)


title: SLAM Project Data: Synthetic Lethality with yhr198c

description:
Internal identifier for dataset: SL/090226/15
Original datafile name: 090226NG142349A03_532.pair
Original datafile2 name: 090226NG142349A03_635.pair
SD of values from reference genes: 0.722
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 0.63 1.08 1.17
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 218 well E11 (strain 22892)


title: SLAM Project Data: Synthetic Lethality with yhr182w

description:
Internal identifier for dataset: SL/090226/3
Original datafile name: 090226NG142346A03_532.pair
Original datafile2 name: 090226NG142346A03_635.pair
SD of values from reference genes: 0.374
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.54 1.90 1.94
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 218 well E04 (strain 22876)


title: SLAM Project Data: Synthetic Lethality with gim5

description:
Internal identifier for dataset: SL/050729/8
Original datafile name: 050729_241_B01.gpr
Original datafile2 name: <none>
SD of values from reference genes: 0.457
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.60 1.18 1.72
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).


title: SLAM Project Data: Synthetic Lethality with gic1

description:
Internal identifier for dataset: SL/070711/8
Original datafile name: 070711_217_E10_8.gpr
Original datafile2 name: <none>
SD of values from reference genes: 0.396
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.90 1.28 1.91
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 217 well E10 (strain 21889)


title: SLAM Project Data: Synthetic Lethality with gfd1

description:
Internal identifier for dataset: SL/050614/4
Original datafile name: 050614_204_H05.gpr
Original datafile2 name: <none>
SD of values from reference genes: 0.367
QueryDeletion::URA3 selection scores (want >> 1; UpTag|DnTag|combined): 1.75 2.18 2.38
For methodological details, please see the following PubMed IDs: 9483801 (BY4743 strain background), 10436161 (Yeast Knockout strain collection), 11743205 (mating-type-specific selection), 15525520 (the "dSLAM" selection scheme), 15994458 (asymmetric PCR), 17060357 (spatial normalization), 17189863 (Methods paper), 18392971 (Methods Mol. Biol. chapter).

value: Plate 204 well H05 (strain 20841)