title: irStress_95unrelated_GM12739_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12739 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12739


title: irStress_95unrelated_GM12767_2hr

status: Public on Mar 28 2013

type: RNA

city: Philadelphia

first: Renuka

last: Nayak

state: PA

address: 3516 Civic Center Blvd

country: USA

institute: HHMI, University of Pennsylvania

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12767 2 hours after exposure.

hyb_protocol: Following fragmentation, 10 ug of cRNA were hybridized for 16-18 hr at 45C on Affymetrix GeneChip Human U133A 2.0 Chip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.

scan_protocol: GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.

data_processing: The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix print default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.

label: biotin

molecule: total RNA

organism: Homo sapiens

source_name: Lymphoblastoid cell lines derived from GM12767

label_protocol: Biotinylated cRNA was prepared using the MessageAmp II-Biotin Enhanced Single Round aRNA Amplification Kit (Ambion/Applied Biosystems) from 1 microg of total RNA following manufacturer's instructions.

growth_protocol: The cells were grown at a density of 500,000 cells/ ml in RPMI 1640 with 15% FBS, 1% penicillin-streptomycin, 2mM L-glutamine at 37°C in a humidified 5% CO2 chamber.

extract_protocol: RNA was extracted using the Rneasy Mini-Kit (Qiagen) as per the manufacturer's instructions

treatment_protocol: 10 Gy of ionizing radiation (IR) in a 137Cs irradiator.

tag: cell line

tag: treatment

value: 10 Gy ionizing radiation

tag: time


title: NB_E_220

city: Heidelberg

first: Thomas

last: Wolf

address: Im Neuenheimer Feld 580

country: Germany

institute: DKFZ

hyb_protocol: Hybridization was performed following the manufacturer's protocol. In brief, 1650 ng of Cy3-labeled cRNA was hybridized on 4x44K custom microarrays using Agilent's High-RPM Gene Expression Hyb Kit. Hybridization was performed for 17 hours at 65°C in a rotating hyb oven at 10 rpm according the company's recommendations.

scan_protocol: After washing and scanning, resulting TIFF-images were processed using Agilent's Feature Extraction software Version 9.5.1.

data_processing: Data normalization using the quantile algorithm, Reference: G. K. Smyth. Limma: linear models for microarray data. In: R. Gentleman, V. Carey, S. Dudoit, R. Irizarry, W. Huber (eds.) Bioinformatics and Computational Biology Solutions using R and Bioconductor. Springer, New York, 2005. pp. 397-420.

source_name: tumor sample

label_protocol: Labeling was performed according to Agilent's recommentdations. In brief, 1µg total of tumor RNA was linearily amplified and labeled with Cy3 using Agilent's one-color Quick Amp Labeling Kit following the instructions of the protocol.

extract_protocol: 30-60mg of snap-frozen neuroblastoma specimen was cryo-sliced and were homogenized in TRIzol reagent (Invitrogen, Karlsruhe, Germany) using the FastPrep FP120 cell disruptor (Qbiogene, Inc, Carlsbad, CA, USA).Total RNA was isolated following the TRIzol protocol (Invitrogen) and RNA integrity was assessed using the 2100 Bioanalyzer (Agilent, Waldbronn, Germany). Only samples with an RNA Integrity Number >7.5 were taken for further analysis.

treatment_protocol: All neuroblastoma samples of this set were obtained prior to any cytotoxic treatment and were snap-frozen immediately after surgery. Prior to RNA extraction tumor cell content was checked by a pathologist and only samples with >60% tumor content were processed further.

tag: tissue

value: Neuroblastoma

tag: Sex

value: F

tag: mycn (1

value: not amplified; Amp:amplified; n.a.:not available):1

tag: Stage

tag: age (1

value: <18 months; 2:>18 months):2


title: NB_E_226

value: M


title: irStress_95unrelated_GM12751_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12751 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12751


title: irStress_95unrelated_GM12750_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12750 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12750


title: NB_E_217


title: irStress_95unrelated_GM12717_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12717 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12717


title: NB_E_216

value: <18 months; 2:>18 months):1


title: NB_E_218

value: not amplified; Amp:amplified; n.a.:not available):Amp


title: irStress_95unrelated_GM12470_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12470 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12470


title: irStress_95unrelated_GM12708_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12708 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12708


title: NB_E_215


title: irStress_95unrelated_GM12471_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12471 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12471


title: NB_E_214


title: irStress_95unrelated_GM12456_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12456 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12456


title: NB_E_213


title: NB_E_212


title: NB_E_211


title: NB_E_210


title: irStress_95unrelated_GM12386_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12386 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12386


title: irStress_95unrelated_GM12385_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12385 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12385


title: irStress_95unrelated_GM12248_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12248 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12248


title: irStress_95unrelated_GM12249_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12249 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12249


title: irStress_95unrelated_GM12239_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12239 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12239


title: NB_E_207


title: NB_E_208


title: irStress_95unrelated_GM12234_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12234 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12234


title: irStress_95unrelated_GM12156_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12156 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12156


title: NB_E_206


title: NB_E_205


title: NB_E_209


title: irStress_95unrelated_GM12236_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12236 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12236


title: irStress_95unrelated_GM12155_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12155 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12155


title: NB_E_204

source_name: tumor sample


title: NB_E_203


title: irStress_95unrelated_GM12146_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12146 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12146


title: irStress_95unrelated_GM12154_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12154 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12154


title: NB_E_200


title: NB_E_202


title: irStress_95unrelated_GM12145_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12145 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12145


title: NB_E_197


title: irStress_95unrelated_GM12056_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12056 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12056


title: irStress_95unrelated_GM12006_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12006 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12006


title: NB_E_199


title: NB_E_198


title: irStress_95unrelated_GM12005_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12005 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12005


title: irStress_95unrelated_GM12004_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12004 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12004


title: NB_E_194


title: irStress_95unrelated_GM12144_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12144 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12144


title: irStress_95unrelated_GM12057_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12057 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12057


title: irStress_95unrelated_GM11994_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM11994 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM11994


title: NB_E_195


title: NB_E_190


title: NB_E_201


title: irStress_95unrelated_GM12003_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12003 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12003


title: NB_E_193


title: NB_E_191


title: irStress_95unrelated_GM11995_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM11995 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM11995


title: NB_E_192


title: irStress_95unrelated_GM11993_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM11993 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM11993


title: NB_E_188


title: irStress_95unrelated_GM11992_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM11992 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM11992


title: irStress_95unrelated_GM11882_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM11882 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM11882


title: irStress_95unrelated_GM11881_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM11881 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM11881


title: NB_E_196

source_name: tumor sample


title: irStress_95unrelated_GM11839_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM11839 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM11839


title: NB_E_189


title: irStress_95unrelated_GM11832_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM11832 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM11832


title: NB_E_183


title: NB_E_182


title: irStress_95unrelated_GM11829_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM11829 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM11829


title: NB_E_185


title: NB_E_184


title: NB_E_187


title: NB_E_180


title: irStress_95unrelated_GM11840_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM11840 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM11840


title: irStress_95unrelated_GM11830_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM11830 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM11830


title: irStress_95unrelated_GM10858_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM10858 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM10858


title: irStress_95unrelated_GM10857_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM10857 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM10857


title: irStress_95unrelated_GM11831_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM11831 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM11831


title: NB_E_222


title: irStress_95unrelated_GM10853_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM10853 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM10853


title: irStress_95unrelated_GM12812_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12812 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12812


title: NB_E_179


title: NB_E_181


title: NB_E_177


title: irStress_95unrelated_GM10852_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM10852 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM10852


title: NB_E_176


title: NB_E_175


title: irStress_95unrelated_GM10844_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM10844 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM10844


title: NB_E_178


title: irStress_95unrelated_GM10850_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM10850 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM10850


title: irStress_95unrelated_GM10845_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM10845 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM10845


title: NB_E_174


title: NB_E_172


title: NB_E_171


title: irStress_95unrelated_GM10848_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM10848 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM10848


title: irStress_95unrelated_GM10843_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM10843 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM10843


title: irStress_95unrelated_GM10849_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM10849 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM10849


title: irStress_95unrelated_GM10842_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM10842 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM10842


title: NB_E_170

source_name: tumor sample


title: irStress_95unrelated_GM10837_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM10837 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM10837


title: NB_E_168


title: irStress_95unrelated_GM10834_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM10834 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM10834


title: NB_E_169


title: irStress_95unrelated_GM10840_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM10840 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM10840


title: irStress_95unrelated_GM10841_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM10841 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM10841


title: irStress_95unrelated_GM10832_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM10832 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM10832


title: irStress_95unrelated_GM10836_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM10836 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM10836


title: NB_E_166


title: NB_E_164


title: NB_E_163


title: NB_E_162


title: irStress_95unrelated_GM10833_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM10833 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM10833


title: NB_E_165


title: irStress_95unrelated_GM07357_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM07357 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM07357


title: irStress_95unrelated_GM07345_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM07345 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM07345


title: irStress_95unrelated_GM07057_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM07057 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM07057


title: NB_E_159


title: irStress_95unrelated_GM07055_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM07055 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM07055


title: irStress_95unrelated_GM07056_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM07056 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM07056


title: NB_E_157


title: NB_E_160

value: not amplified; Amp:amplified; n.a.:not available):n.a.


title: irStress_95unrelated_GM07049_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM07049 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM07049


title: NB_E_161


title: NB_E_158


title: NB_E_156


title: irStress_95unrelated_GM07034_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM07034 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM07034


title: irStress_95unrelated_GM06994_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM06994 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM06994


title: irStress_95unrelated_GM06993_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM06993 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM06993


title: irStress_95unrelated_GM07002_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM07002 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM07002


title: irStress_95unrelated_GM06990_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM06990 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM06990


title: irStress_95unrelated_GM07022_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM07022 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM07022


title: NB_E_151

source_name: tumor sample


title: irStress_95unrelated_GM06985_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM06985 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM06985


title: NB_E_150


title: irStress_95unrelated_GM12911_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12911 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12911


title: NB_E_148


title: NB_E_147


title: irStress_95unrelated_GM12912_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12912 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12912


title: irStress_95unrelated_GM07000_2hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM07000 2 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM07000


title: NB_E_149


title: NB_E_167


title: irStress_95unrelated_GM12897_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12897 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12897


title: irStress_95unrelated_GM12892_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12892 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12892


title: irStress_95unrelated_GM12898_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12898 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12898


title: NB_E_146


title: NB_E_142


title: NB_E_155


title: NB_E_154


title: NB_E_186


title: NB_E_152


title: irStress_95unrelated_GM12891_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12891 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12891


title: NB_E_144


title: NB_E_145


title: NB_E_153


title: irStress_95unrelated_GM12872_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12872 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12872


title: NB_E_139


title: irStress_95unrelated_GM12874_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12874 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12874


title: NB_E_137


title: NB_E_141


title: NB_E_140


title: NB_E_143


title: irStress_95unrelated_GM12831_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12831 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12831


title: irStress_95unrelated_GM12818_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12818 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12818


title: NB_E_138


title: NB_E_136


title: irStress_95unrelated_GM12832_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12832 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12832


title: irStress_95unrelated_GM12812_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12812 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12812


title: irStress_95unrelated_GM12873_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12873 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12873


title: irStress_95unrelated_GM12813_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12813 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12813


title: NB_E_135

source_name: tumor sample


title: NB_E_132


title: NB_E_133


title: NB_E_134


title: irStress_95unrelated_GM12817_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12817 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12817


title: NB_E_129


title: irStress_95unrelated_GM12814_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12814 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12814


title: NB_E_130


title: irStress_95unrelated_GM12767_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12767 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12767


title: irStress_95unrelated_GM12815_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12815 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12815


title: NB_E_131


title: NB_E_128


title: irStress_95unrelated_GM12750_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12750 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12750


title: NB_E_126


title: irStress_95unrelated_GM12751_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12751 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12751


title: NB_E_124


title: irStress_95unrelated_GM12763_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12763 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12763


title: NB_E_123


title: NB_E_173


title: irStress_95unrelated_GM12761_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12761 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12761


title: irStress_95unrelated_GM12766_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12766 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12766


title: irStress_95unrelated_GM12762_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12762 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12762


title: NB_E_127


title: irStress_95unrelated_GM12760_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12760 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12760


title: irStress_95unrelated_GM12739_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12739 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12739


title: irStress_95unrelated_GM12708_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12708 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12708


title: NB_E_125


title: NB_E_119


title: NB_E_122


title: NB_E_120


title: irStress_95unrelated_GM12471_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12471 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12471


title: irStress_95unrelated_GM12470_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12470 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12470


title: irStress_95unrelated_GM12386_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12386 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12386


title: NB_E_115

source_name: tumor sample


title: irStress_95unrelated_GM12456_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12456 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12456


title: NB_E_118


title: irStress_95unrelated_GM12385_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12385 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12385


title: NB_E_121


title: NB_E_117


title: irStress_95unrelated_GM12717_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12717 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12717


title: NB_E_111


title: NB_E_116


title: NB_E_112


title: irStress_95unrelated_GM12156_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12156 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12156


title: NB_E_114


title: NB_E_113


title: irStress_95unrelated_GM12248_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12248 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12248


title: irStress_95unrelated_GM12236_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12236 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12236


title: CTRL7

city: Valencia

first: Francisco

middle: García

last: García

address: Eduardo Primo Yúfera, 3

department: Bionformatics & Biostatistics Unit

country: Spain

institute: CIPF

description: Gene expression data from Human. Control 7

hyb_protocol: Hybridization was performed using 5 μg of biotinylated target, which was incubated with the GeneChip® Human Exon 1.0 ST array (Affymetrix) at 60 rpm in the GeneChip® hybridization oven (Affymetrix) at 45°C for 16 hours. Following hybridization, non-specifically bound material was removed by washing and specifically bound targets were detected using the GeneChip® Hybridization, Wash and Stain kit, and the GeneChip® Fluidics Station 450 (Affymetrix).

scan_protocol: Array scanning was performed according to the manufacturer's instruction (Affymetrix) using the GeneChip Scanner 3000 7G (Affymetrix).

data_processing:
Raw data were processed with the Expression Console software v.1.1 (Affymetrix) for background correction and normalization. The number of genes evaluated was reduced by applying an interquartile filter followed by a detected above background filter to remove the non significant probe sets (i.e. those not expressed and those not changing). The filtering procedure and statistical evaluations were performed with oneChannelGUI graphical interface package to Bioconductor tools.
HuEx-1_0-st-v2.pgf
HuEx-1_0-st-v2.r2.dt1.hg18.extended.mps

source_name: Control 7

label_protocol: Total RNA was subjected to rRNA removal procedure with the RiboMinus Human/Mouse Transcriptome Isolation Kit  (Invitrogen), and cDNA was synthesized using the GeneChip® WT Sense Target Labeling and Control Reagents kit as described by the manufacturer (Affymetrix). The sense cDNA was then fragmented by UDG  and APE 1 ) and biotin-labeled with TdT  using the GeneChip® WT Terminal labeling kit (Affymetrix).

growth_protocol: Whole-blood cell drawn from an antecubital vein at six time points following STEMI diagnosis. The first time (T0) was on patient admission to the Emergency Unit prior to receiving any medication. The second time (T2) blood collection occurred after successful mechanical reperfusion and stenting of the infarct artery within 2 h after T0.  Collections for the subsequent four time points were at 12 h (T12), 24 h (T24), 36 h (T36) and 48 h (T48). For the control group, peripheral blood was collected at one time point.

extract_protocol: Total RNA was isolated from whole blood samples with the PAXGene Blood RNA system (PreAnalytiX) following the manufacturer’s protocols including a RNase-free DNase (Qiagen) step to remove genomic DNA.

tag: disease status

value: acute coronary syndrome (early stage acute myocardial infarction)

tag: cell type

value: peripheral blood cells

tag: time point

value: Control


title: irStress_95unrelated_GM12155_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12155 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12155


title: NB_E_108


title: CTRL3

description: Gene expression data from Human. Control 3

source_name: Control 3


title: irStress_95unrelated_GM12239_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12239 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12239


title: irStress_95unrelated_GM12234_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12234 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12234


title: irStress_95unrelated_GM12249_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12249 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12249


title: NB_E_110


title: JT6

description: Gene expression data from Human. Patient J, time 6

source_name: Patient J, time 6

value: Time 6


title: NB_E_107


title: NB_E_109


title: CTRL1

description: Gene expression data from Human. Control 1

source_name: Control 1


title: irStress_95unrelated_GM12146_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12146 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12146


title: CTRL4

description: Gene expression data from Human. Control 4

source_name: Control 4


title: JT5

description: Gene expression data from Human. Patient J, time 5

source_name: Patient J, time 5

value: Time 5


title: irStress_95unrelated_GM12154_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12154 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12154


title: CTRL6

description: Gene expression data from Human. Control 6

source_name: Control 6


title: NB_E_106

source_name: tumor sample


title: JT3

description: Gene expression data from Human. Patient J, time 3

source_name: Patient J, time 3

value: Time 3


title: NB_E_105


title: CTRL2

description: Gene expression data from Human. Control 2

source_name: Control 2


title: JT4

description: Gene expression data from Human. Patient J, time 4

source_name: Patient J, time 4

value: Time 4


title: irStress_95unrelated_GM12145_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12145 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12145


title: irStress_95unrelated_GM12144_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12144 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12144


title: NB_E_104


title: irStress_95unrelated_GM12057_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12057 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12057


title: NB_E_103


title: JT2

description: Gene expression data from Human. Patient J, time 2

source_name: Patient J, time 2

value: Time 2


title: IT5

description: Gene expression data from Human. Patient I, time 5

source_name: Patient I, time 5


title: irStress_95unrelated_GM12877_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12877 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12877

value: 10 Gy ionizing radiation


title: irStress_95unrelated_GM12056_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12056 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12056


title: IT6

description: Gene expression data from Human. Patient I, time 6

source_name: Patient I, time 6


title: IT1

description: Gene expression data from Human. Patient I, time 1

source_name: Patient I, time 1

value: Time 1


title: NB_E_100

value: Neuroblastoma


title: JT1

description: Gene expression data from Human. Patient J, time 1

source_name: Patient J, time 1


title: irStress_95unrelated_GM12005_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12005 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12005


title: IT2

description: Gene expression data from Human. Patient I, time 2

source_name: Patient I, time 2


title: IT4

description: Gene expression data from Human. Patient I, time 4

source_name: Patient I, time 4


title: NB_E_102

source_name: tumor sample

value: not amplified; Amp:amplified; n.a.:not available):1


title: HT2

description: Gene expression data from Human. Patient H, time 2

source_name: Patient H, time 2


title: irStress_95unrelated_GM12004_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12004 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12004


title: NB_E_99

value: F

value: <18 months; 2:>18 months):2


title: irStress_95unrelated_GM12006_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12006 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12006


title: NB_E_98

value: <18 months; 2:>18 months):1


title: HT3

description: Gene expression data from Human. Patient H, time 3

source_name: Patient H, time 3


title: irStress_95unrelated_GM12003_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM12003 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM12003


title: FT2

description: Gene expression data from Human. Patient F, time 2

source_name: Patient F, time 2


title: FT3

description: Gene expression data from Human. Patient F, time 3

source_name: Patient F, time 3


title: HT6

description: Gene expression data from Human. Patient H, time 6

source_name: Patient H, time 6


title: HT5

description: Gene expression data from Human. Patient H, time 5

source_name: Patient H, time 5


title: FT4

description: Gene expression data from Human. Patient F, time 4

source_name: Patient F, time 4


title: NB_E_96

value: M


title: NB_E_101


title: FT5

description: Gene expression data from Human. Patient F, time 5

source_name: Patient F, time 5


title: ET5

description: Gene expression data from Human. Patient E, time 5

source_name: Patient E, time 5


title: ET3

description: Gene expression data from Human. Patient E, time 3

source_name: Patient E, time 3


title: NB_E_97


title: ET6

description: Gene expression data from Human. Patient E, time 6

source_name: Patient E, time 6


title: irStress_95unrelated_GM11994_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM11994 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM11994


title: FT1

description: Gene expression data from Human. Patient F, time 1

source_name: Patient F, time 1


title: NB_E_95

source_name: tumor sample


title: irStress_95unrelated_GM11993_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM11993 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM11993


title: ET4

description: Gene expression data from Human. Patient E, time 4

source_name: Patient E, time 4


title: NB_E_93


title: NB_E_94


title: irStress_95unrelated_GM11995_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM11995 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM11995


title: ET1

description: Gene expression data from Human. Patient E, time 1

source_name: Patient E, time 1


title: irStress_95unrelated_GM11882_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM11882 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM11882


title: irStress_95unrelated_GM11992_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM11992 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM11992


title: DT2

description: Gene expression data from Human. Patient D, time 2

source_name: Patient D, time 2


title: DT3

description: Gene expression data from Human. Patient D, time 3

source_name: Patient D, time 3


title: DT6

description: Gene expression data from Human. Patient D, time 6

source_name: Patient D, time 6


title: DT4

description: Gene expression data from Human. Patient D, time 4

source_name: Patient D, time 4


title: CT6

description: Gene expression data from Human. Patient C, time 6

source_name: Patient C, time 6


title: DT5

description: Gene expression data from Human. Patient D, time 5

source_name: Patient D, time 5


title: NB_E_92


title: irStress_95unrelated_GM11881_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM11881 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM11881


title: DT1

description: Gene expression data from Human. Patient D, time 1

source_name: Patient D, time 1


title: irStress_95unrelated_GM11839_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM11839 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM11839


title: CT2

description: Gene expression data from Human. Patient C, time 2

source_name: Patient C, time 2


title: NB_E_90

source_name: tumor sample


title: BT6

description: Gene expression data from Human. Patient B, time 6

source_name: Patient B, time 6


title: CT1

description: Gene expression data from Human. Patient C, time 1

source_name: Patient C, time 1


title: irStress_95unrelated_GM11840_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM11840 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM11840


title: CT4

description: Gene expression data from Human. Patient C, time 4

source_name: Patient C, time 4


title: CT5

description: Gene expression data from Human. Patient C, time 5

source_name: Patient C, time 5


title: NB_E_89

value: not amplified; Amp:amplified; n.a.:not available):Amp


title: BT5

description: Gene expression data from Human. Patient B, time 5

source_name: Patient B, time 5

value: acute coronary syndrome (early stage acute myocardial infarction)


title: NB_E_91


title: BT3

description: Gene expression data from Human. Patient B, time 3

source_name: Patient B, time 3

value: peripheral blood cells


title: BT4

description: Gene expression data from Human. Patient B, time 4

source_name: Patient B, time 4


title: BT2

description: Gene expression data from Human. Patient B, time 2

source_name: Patient B, time 2


title: irStress_95unrelated_GM11830_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM11830 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM11830


title: BT1

description: Gene expression data from Human. Patient B, time 1

source_name: Patient B, time 1


title: NB_E_88


title: irStress_95unrelated_GM11831_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM11831 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM11831


title: irStress_95unrelated_GM11832_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM11832 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM11832


title: NB_E_85


title: irStress_95unrelated_GM10858_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM10858 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM10858


title: CT3

description: Gene expression data from Human. Patient C, time 3

source_name: Patient C, time 3


title: AT3

description: Gene expression data from Human. Patient A, time 3

source_name: Patient A, time 3


title: irStress_95unrelated_GM11829_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM11829 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM11829


title: AT5

description: Gene expression data from Human. Patient A, time 5

source_name: Patient A, time 5


title: AT2

description: Gene expression data from Human. Patient A, time 2

source_name: Patient A, time 2


title: NB_E_86

source_name: tumor sample


title: AT6

description: Gene expression data from Human. Patient A, time 6

source_name: Patient A, time 6


title: AT4

description: Gene expression data from Human. Patient A, time 4

source_name: Patient A, time 4


title: NB_E_87


title: irStress_95unrelated_GM10853_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM10853 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM10853


title: irStress_95unrelated_GM10857_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM10857 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM10857


title: irStress_95unrelated_GM10849_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM10849 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM10849


title: AT1

description: Gene expression data from Human. Patient A, time 1

source_name: Patient A, time 1


title: irStress_95unrelated_GM10852_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM10852 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM10852


title: NB_E_84


title: irStress_95unrelated_GM10850_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM10850 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM10850


title: MI-CP200 scleroderma subject P25, Day 85, 10.0 mpk

city: Gaithersburg

first: Christopher

middle: Allen

last: Morehouse

state: MD

address: One Medimmune Way

institute: Medimmune LLC

hyb_protocol: Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HGU133 Plus 2.0 Array. GeneChips were washed and stained in an Affymetrix Fluidics Station 450.

data_processing: The data were analyzed in R.

source_name: PAXgene Whole Blood

label_protocol: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).

extract_protocol: Qiagen PAXgene Blood RNA Kit extraction of total RNA was performed according to the manufacturer's instructions.

treatment_protocol: Escalating single IV doses of MEDI-551 (anti-CD19) in adult subjects with scleroderma. In this study, 5 cohorts of subjects received 1 of 5 single IV doses of MEDI-551 (0.1, 0.3, 1.0, 3.0, or 10.0 mg/kg) or placebo.

tag: phenotype

value: scleroderma subject

value: whole blood

tag: timepoint

value: Day 85


title: NB_E_83


title: MI-CP200 scleroderma subject P25, Day 29, 10.0 mpk

value: Day 29


title: MI-CP200 scleroderma subject P24, Day 85, 10.0 mpk


title: MI-CP200 scleroderma subject P25, Day 1 (pre-dose), 10.0 mpk

value: Day 1 (pre-dose)


title: MI-CP200 scleroderma subject P24, Day 3, 10.0 mpk

value: Day 3


title: NB_E_82


title: MI-CP200 scleroderma subject P24, Day 1 (pre-dose), 10.0 mpk


title: MI-CP200 scleroderma subject P23, Day 85, Placebo

value: Placebo


title: NB_E_81


title: MI-CP200 scleroderma subject P24, Day 29, 10.0 mpk


title: NB_E_80


title: NB_E_79

value: not amplified; Amp:amplified; n.a.:not available):n.a.


title: irStress_95unrelated_GM10843_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM10843 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM10843

value: 10 Gy ionizing radiation


title: MI-CP200 scleroderma subject P23, Day 3, Placebo


title: MI-CP200 scleroderma subject P23, Day 1 (pre-dose), Placebo


title: NB_E_78

value: Neuroblastoma

value: not amplified; Amp:amplified; n.a.:not available):1


title: irStress_95unrelated_GM10848_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM10848 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM10848


title: irStress_95unrelated_GM10844_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM10844 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM10844


title: MI-CP200 scleroderma subject P22, Day 29, 10.0 mpk


title: irStress_95unrelated_GM10842_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM10842 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM10842


title: MI-CP200 scleroderma subject P22, Day 85, 10.0 mpk


title: MI-CP200 scleroderma subject P23, Day 29, Placebo


title: irStress_95unrelated_GM10845_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM10845 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM10845


title: MI-CP200 scleroderma subject P25, Day 3, 10.0 mpk


title: MI-CP200 scleroderma subject P22, Day 3, 10.0 mpk


title: NB_E_77

value: F


title: MI-CP200 scleroderma subject P22, Day 1 (pre-dose), 10.0 mpk


title: NB_E_74

value: <18 months; 2:>18 months):2


title: irStress_95unrelated_GM10841_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM10841 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM10841


title: MI-CP200 scleroderma subject P21, Day 29, 10.0 mpk


title: NB_E_73

value: M

value: <18 months; 2:>18 months):1


title: irStress_95unrelated_GM10840_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM10840 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM10840


title: NB_E_72

source_name: tumor sample

value: not amplified; Amp:amplified; n.a.:not available):Amp


title: NB_E_71


title: MI-CP200 scleroderma subject P20, Day 1 (pre-dose), 10.0 mpk


title: MI-CP200 scleroderma subject P19, Day 3, 10.0 mpk


title: MI-CP200 scleroderma subject P21, Day 1 (pre-dose), 10.0 mpk


title: NB_E_75


title: NB_E_76


title: MI-CP200 scleroderma subject P19, Day 1 (pre-dose), 10.0 mpk


title: MI-CP200 scleroderma subject P20, Day 85, 10.0 mpk


title: irStress_95unrelated_GM10832_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM10832 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM10832


title: NB_E_68


title: NB_E_70


title: irStress_95unrelated_GM10833_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM10833 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM10833


title: MI-CP200 scleroderma subject P18, Day 85, Placebo


title: irStress_95unrelated_GM10834_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM10834 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM10834


title: irStress_95unrelated_GM10836_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM10836 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM10836


title: MI-CP200 scleroderma subject P21, Day 3, 10.0 mpk


title: MI-CP200 scleroderma subject P19, Day 85, 10.0 mpk


title: MI-CP200 scleroderma subject P20, Day 3, 10.0 mpk


title: irStress_95unrelated_GM10837_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM10837 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM10837


title: MI-CP200 scleroderma subject P19, Day 29, 10.0 mpk


title: MI-CP200 scleroderma subject P20, Day 29, 10.0 mpk


title: NB_E_69


title: irStress_95unrelated_GM07357_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM07357 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM07357


title: NB_E_67


title: irStress_95unrelated_GM07057_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM07057 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM07057


title: MI-CP200 scleroderma subject P18, Day 1 (pre-dose), Placebo


title: irStress_95unrelated_GM07345_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM07345 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM07345


title: irStress_95unrelated_GM07056_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM07056 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM07056


title: NB_E_66


title: MI-CP200 scleroderma subject P17, Day 85, 3.0 mpk

source_name: PAXgene Whole Blood

value: 3.0 mpk MEDI-551


title: MI-CP200 scleroderma subject P18, Day 3, Placebo

value: scleroderma subject


title: NB_E_65


title: MI-CP200 scleroderma subject P18, Day 29, Placebo

value: whole blood


title: MI-CP200 scleroderma subject P17, Day 29, 3.0 mpk


title: MI-CP200 scleroderma subject P17, Day 1 (pre-dose), 3.0 mpk


title: MI-CP200 scleroderma subject P17, Day 3, 3.0 mpk


title: irStress_95unrelated_GM07049_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM07049 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM07049


title: NB_E_64


title: MI-CP200 scleroderma subject P15, Day 85, 3.0 mpk

value: Day 85


title: MI-CP200 scleroderma subject P16, Day 29, 3.0 mpk

value: Day 29


title: NB_E_62


title: MI-CP200 scleroderma subject P16, Day 3, 3.0 mpk


title: irStress_95unrelated_GM07055_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM07055 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM07055


title: irStress_95unrelated_GM07034_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM07034 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM07034


title: NB_E_61


title: MI-CP200 scleroderma subject P16, Day 85, 3.0 mpk


title: MI-CP200 scleroderma subject P15, Day 29, 3.0 mpk


title: MI-CP200 scleroderma subject P16, Day 1 (pre-dose), 3.0 mpk

value: Day 1 (pre-dose)


title: MI-CP200 scleroderma subject P15, Day 1 (pre-dose), 3.0 mpk


title: irStress_95unrelated_GM07022_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM07022 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM07022


title: NB_E_60


title: MI-CP200 scleroderma subject P21, Day 85, 10.0 mpk


title: MI-CP200 scleroderma subject P15, Day 3, 3.0 mpk

value: Day 3


title: irStress_95unrelated_GM07002_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM07002 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM07002


title: MI-CP200 scleroderma subject P13, Day 85, Placebo

value: Placebo


title: MI-CP200 scleroderma subject P14, Day 29, 3.0 mpk


title: NB_E_59


title: MI-CP200 scleroderma subject P13, Day 3, Placebo


title: MI-CP200 scleroderma subject P14, Day 3, 3.0 mpk


title: MI-CP200 scleroderma subject P14, Day 1 (pre-dose), 3.0 mpk


title: NB_E_57


title: MI-CP200 scleroderma subject P13, Day 1 (pre-dose), Placebo


title: irStress_95unrelated_GM06994_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM06994 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM06994


title: MI-CP200 scleroderma subject P13, Day 29, Placebo


title: irStress_95unrelated_GM07000_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM07000 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM07000


title: MI-CP200 scleroderma subject P12, Day 3, 1.0 mpk

value: 1.0 mpk MEDI-551


title: NB_E_56

source_name: tumor sample


title: NB_E_58


title: MI-CP200 scleroderma subject P12, Day 29, 1.0 mpk


title: irStress_95unrelated_GM06990_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM06990 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM06990

value: 10 Gy ionizing radiation


title: NB_E_63

value: Neuroblastoma


title: MI-CP200 scleroderma subject P12, Day 85, 1.0 mpk


title: irStress_95unrelated_GM06993_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM06993 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM06993


title: MI-CP200 scleroderma subject P12, Day 1 (pre-dose), 1.0 mpk


title: MI-CP200 scleroderma subject P11, Day 1 (pre-dose), 1.0 mpk


title: NB_E_55

value: not amplified; Amp:amplified; n.a.:not available):1


title: NB_E_54


title: NB_E_52

value: F


title: NB_E_53


title: MI-CP200 scleroderma subject P11, Day 3, 1.0 mpk


title: MI-CP200 scleroderma subject P11, Day 29, 1.0 mpk


title: MI-CP200 scleroderma subject P11, Day 85, 1.0 mpk


title: MI-CP200 scleroderma subject P10, Day 85, 1.0 mpk


title: MI-CP200 scleroderma subject P10, Day 29, 1.0 mpk


title: MI-CP200 scleroderma subject P09, Day 85, 1.0 mpk


title: NB_E_51


title: MI-CP200 scleroderma subject P10, Day 1 (pre-dose), 1.0 mpk


title: MI-CP200 scleroderma subject P09, Day 29, 1.0 mpk


title: NB_E_50


title: MI-CP200 scleroderma subject P09, Day 1 (pre-dose), 1.0 mpk


title: MI-CP200 scleroderma subject P08, Day 85, 1.0 mpk


title: MI-CP200 scleroderma subject P10, Day 3, 1.0 mpk


title: Atseedlings_6h_ABA_rep2

city: Harbin

first: Yujia

last: Liu

address: 26 Hexing Road

country: China

institute: Northeast Forestry University

description: Gene expression after 6h in ABA-treatmented Arabidopsis seedlings

hyb_protocol: Each Slide was hybridized with 1.65μg Cy3-labeled cRNA using Gene Expression Hybridization Kit (Cat#5188-5242, Agilent technologies, Santa Clara, CA, US) in Hybridization Oven (Cat#G2545A, Agilent technologies, Santa Clara, CA, US), according to the manufacturer’s  instructions. After 17 hours hybridization, slides were washed in staining dishes (Cat#121, Thermo Shandon, Waltham, MA, US) with Gene Expression Wash Buffer Kit (Cat#5188-5327, Agilent technologies, Santa Clara, CA, US), followed the manufacturer’s instructions.

scan_protocol: Slides were scanned by Agilent Microarray Scanner (Cat#G2565CA, Agilent technologies, Santa Clara, CA, US) with default settings, Dye channel: Green, Scan resolution=5μm, PMT 100%, 10%, 16bit. Feature Extraction software 10.7 (Agilent technologies, Santa Clara, CA, US) Raw data were normalized by Quantile algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US).

data_processing: The scanned images were analyzed with R-software (The R Project for Statistical Computing, http:// www. r-project.org/) of SAS system statistics to obtain background subtracted and spatially detrended Processed Signal intensities.

organism: Arabidopsis thaliana

source_name: Arabidopsis seedlings, 6h, ABA, replicate 2

label_protocol: Total RNA was amplified and labeled by Low Input Quick Amp Labeling Kit, One-Color (Cat#5190-2305, Agilent technologies, Santa Clara, CA, US), followed the manufacturer’s instructions. Labeled cRNA were purified by RNeasy mini kit (Cat#74106, QIAGEN, GmBH, Germany).

growth_protocol: Arabidopsis thaliana ecotypes Columbia (Col-0) seedlings grown in freshwater-irrigated pots filled with the artificial soil (Vermiculite: perlite: soil = 3:1:1, v/v) in a culture condition of 70% (±5%) relative humidity, 16 h 120 μmol•m-2•s-1 light / 8 h dark photoperiod at 22°C (±2°C).

extract_protocol: Total RNA was extracted using TRIZOL Reagent (Cat#15596-018，Life technologies, Carlsbad, CA, US) following the manufacturer’s instructions and checked for a RIN number to inspect RNA integration by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US). Qualified total RNA was further purified by RNeasy mini kit (Cat#74106, QIAGEN, GmBH, Germany) and RNase-Free DNase Set (Cat#79254, QIAGEN, GmBH, Germany).

treatment_protocol: Four-week-old Arabidopsis thaliana ecotypes Columbia (Col-0) were treated with either 150 mM NaCl or 10 μM ABA for 6 hours. The whole seedlings including root and shoot were harvested and pooled for each treatment before freezing in liquid nitrogen and storage at -80ºC for RNA isolation.

value: whole plant

tag: ecotype

tag: age

value: 4 week-old seedlings

value: ABA-stressed


title: MI-CP200 scleroderma subject P09, Day 3, 1.0 mpk


title: MI-CP200 scleroderma subject P07, Day 85, 1.0 mpk


title: Atseedlings_6h_NaCl_rep3

description: Gene expression after 6h in NaCl-treatmented Arabidopsis seedlings

source_name: Arabidopsis seedlings, 6h, NaCl, replicate 3

value: salt-stressed


title: MI-CP200 scleroderma subject P08, Day 3, 1.0 mpk


title: MI-CP200 scleroderma subject P08, Day 1 (pre-dose), 1.0 mpk


title: MI-CP200 scleroderma subject P08, Day 29, 1.0 mpk


title: NB_E_48

value: <18 months; 2:>18 months):2


title: irStress_95unrelated_GM06985_0hr

description: Gene expression data from lymphoblastoid cells following 10 Gy irradiation in a 137Cs irradiator from GM06985 0 hours after exposure.

source_name: Lymphoblastoid cell lines derived from GM06985


title: Atseedlings_6h_NaCl_rep2

source_name: Arabidopsis seedlings, 6h, NaCl, replicate 2


title: Atseedlings_6h_ABA_rep3

source_name: Arabidopsis seedlings, 6h, ABA, replicate 3


title: NB_E_49


title: Atseedlings_6h_NaCl_rep1

source_name: Arabidopsis seedlings, 6h, NaCl, replicate 1


title: MI-CP200 scleroderma subject P07, Day 29, 1.0 mpk

source_name: PAXgene Whole Blood

value: scleroderma subject


title: NB_E_45

value: M

value: <18 months; 2:>18 months):1


title: NB_E_47


title: MI-CP200 scleroderma subject P07, Day 3, 1.0 mpk

value: whole blood


title: Atseedlings_6h_ABA_rep1

source_name: Arabidopsis seedlings, 6h, ABA, replicate 1


title: NB_E_46


title: Atseedlings_6h_H2O_rep3

description: Gene expression after 6h in H2O-irrigated Arabidopsis seedlings

source_name: Arabidopsis seedlings, 6h, H2O, replicate 3

value: normal conditions


title: Atseedlings_6h_H2O_rep2

source_name: Arabidopsis seedlings, 6h, H2O, replicate 2


title: MI-CP200 scleroderma subject P07, Day 1 (pre-dose), 1.0 mpk

value: Day 1 (pre-dose)


title: NB_E_43


title: NB_E_42


title: MI-CP200 scleroderma subject P05, Day 85, 0.3 mpk

value: 0.3 mpk MEDI-551

value: Day 85


title: MI-CP200 scleroderma subject P06, Day 85, 0.3 mpk


title: MI-CP200 scleroderma subject P06, Day 1 (pre-dose), 0.3 mpk


title: MI-CP200 scleroderma subject P06, Day 3, 0.3 mpk

value: Day 3


title: MI-CP200 scleroderma subject P06, Day 29, 0.3 mpk

value: Day 29


title: NB_E_44

value: Neuroblastoma

value: not amplified; Amp:amplified; n.a.:not available):1


title: MI-CP200 scleroderma subject P04, Day 1 (pre-dose), 0.3 mpk


title: MI-CP200 scleroderma subject P05, Day 29, 0.3 mpk


title: MI-CP200 scleroderma subject P04, Day 29, 0.3 mpk


title: NB_E_38


title: NB_E_39

value: F


title: MI-CP200 scleroderma subject P05, Day 3, 0.3 mpk


title: MI-CP200 scleroderma subject P05, Day 1 (pre-dose), 0.3 mpk


title: MI-CP200 scleroderma subject P04, Day 85, 0.3 mpk


title: MI-CP200 scleroderma subject P03, Day 85, 0.3 mpk


title: MI-CP200 scleroderma subject P03, Day 3, 0.3 mpk


title: MI-CP200 scleroderma subject P03, Day 29, 0.3 mpk


title: NB_E_41


title: NB_E_37

value: not amplified; Amp:amplified; n.a.:not available):Amp


title: NB_E_40


title: MI-CP200 scleroderma subject P03, Day 1 (pre-dose), 0.3 mpk


title: MI-CP200 scleroderma subject P01, Day 85, 0.1 mpk

value: 0.1 mpk MEDI-551


title: MI-CP200 scleroderma subject P02, Day 85, Placebo

value: Placebo


title: MI-CP200 scleroderma subject P04, Day 3, 0.3 mpk


title: MI-CP200 scleroderma subject P02, Day 1 (pre-dose), Placebo


title: MI-CP200 scleroderma subject P01, Day 3, 0.1 mpk


title: MI-CP200 scleroderma subject P01, Day 29, 0.1 mpk


title: MI-CP200 scleroderma subject P02, Day 3, Placebo


title: MI-CP200 scleroderma subject P02, Day 29, Placebo


title: NB_E_35


title: NB_E_36


title: MI-CP200 scleroderma subject P01, Day 1 (pre-dose), 0.1 mpk


title: NB_E_32

value: <18 months; 2:>18 months):2


title: Healthy Donor 24

value: healthy donor


title: Healthy Donor 20


title: NB_E_34


title: NB_E_31


title: Healthy Donor 22


title: Healthy Donor 21


title: NB_E_29

value: M


title: Healthy Donor 18


title: Healthy Donor 19


title: NB_E_30

value: <18 months; 2:>18 months):1


title: Atseedlings_6h_H2O_rep1

source_name: Arabidopsis seedlings, 6h, H2O, replicate 1

value: whole plant

value: 4 week-old seedlings


title: NB_E_28


title: Healthy Donor 16

value: whole blood


title: Healthy Donor 23


title: NB_E_33


title: Healthy Donor 17


title: NB_E_26


title: Healthy Donor 12


title: Healthy Donor 9


title: Healthy Donor 13


title: NB_E_25


title: Healthy Donor 14


title: NB_E_27


title: Healthy Donor 7


title: Healthy Donor 11


title: Healthy Donor 6


title: NB_E_24


title: Healthy Donor 8


title: NB_E_22


title: Healthy Donor 4


title: Healthy Donor 5


title: Healthy Donor 1


title: Healthy Donor 2


title: Healthy Donor 10


title: Healthy Donor 15


title: NB_E_18


title: NB_E_20


title: NB_E_21


title: Donor-2, Whole blood

description: HGU133 Plus array data

data_processing: The data was analyzed in R.

source_name: Whole Blood

label_protocol: Biotinylated cRNA were prepared according to the standard Ambion MessageAmp II-Biotin Enhanced Single Round aRNA Amplification protocol from 75 ng total RNA.

extract_protocol: Zymo RNA MicroPrep kit extraction of total RNA was performed according to the manufacturer's instructions.

treatment_protocol: To examine gene expression in purified cellular fractions, normal human blood was collected from 2 donors as per institutional policy. The granulocyte (CD15+), monocyte (CD14+), T cell (CD3+), B cell (Non-PC gated, CD19+), and PC (CD27++CD38++) fractions from peripheral blood were separated. White blood cells were washed with FACS buffer (PBS + 0.5%BSA + 2mM EDTA (Gibco)) and incubated with 20% heat-inactivated FBS for 10-15 minutes on ice. The following mAbs were added directly to the cells: CD15 (HI98); CD14 (M5E2), CD3 (UCHT1), CD27 (M-T271), CD38 (HB7), and DAPI (Molecular probes). Cells were sorted on a Becton Dickinson FACS Aria II flow cytometer. All sorted fractions were collected in FACS buffer, centrifuged, and the resulting cell pellet was suspended in RNA lysis buffer (Ambion).

value: Whole Blood

tag: markers


title: NB_E_17


title: NB_E_19


title: NB_E_23


title: Healthy Donor 3


title: NB_E_16


title: NB_E_14


title: NB_E_15


title: NB_E_12


title: NB_E_13


title: Donor-1, Whole blood

description: HGU133 Plus array data


title: NB_E_10


title: hd3

city: padova

first: andrea

last: bozzato

address: via bassi

country: Italy

institute: university

description: Gene expression profile comparison from fibroblasts of Huntington individuals and normal ones

hyb_protocol: Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 plus Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.

scan_protocol: The GeneChip was scanned and data extracted using GeneChip scanner 3000 7G (Affimetrix, SantanClara, CA).

data_processing: The data were analyzed with Expression Console™ Software using Affymetrix default analysis settings and Robust Multichip Analysis (RMA). Subsequently analyses for differentially expressed genes cathegorization were performed with OnechannelGUI in R environment

source_name: dermal fibroblast

growth_protocol: Fibroblast cells were cultured in Eagle's minimum essential (MEM) supplemented with 10% FBS, 100 mg/mL streptomycin, 100 U/mL penicillin and incubated at 37 C in a humidified chamber with 5% CO2. Cells were harvested once they reached confluence by treating with trypsin (0.05% trypsin with 0.25% EDTA).

extract_protocol: Trizol extraction of total RNA  followed by Qiagen cleanup were performed according to the manufacturer's instructions.

tag: disease

value: Huntington

value: Fibroblast cells

value: male


title: hd6


title: ctrl3

value: normal


title: hd2


title: hd5


title: hd1


title: hd4


title: erStress_131grandparents_GM13133_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM13133 treated with 4 ug/ml tunicamycin 8 hours.

hyb_protocol: Following fragmentation, 15 ug of cRNA were hybridized for 16-18 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.

source_name: Lymphoblastoid cell lines derived from GM13133

label_protocol: Biotinylated cRNA were prepared according to the Ambion MessageAmp Premier RNA Amplification Kit protocol from 500 ng total RNA (MessageAmp Premier RNA Amplification Kit Instruction Manual, 2008, Ambion).

growth_protocol: The cells were grown at a density of 1 million cells/ ml in RPMI 1640 with 15% FBS, 1% penicillin-streptomycin, 2mM L-glutamine at 37°C in a humidified 5% CO2 chamber.

treatment_protocol: 4 ug/ml tunicamycin 8 hours

tag: cell line

value: 4 ug/ml tunicamycin


title: NB_E_9

source_name: tumor sample

value: Neuroblastoma

value: not amplified; Amp:amplified; n.a.:not available):1


title: ctrl1


title: ctrl2


title: erStress_131grandparents_GM13042_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM13042 treated with DMSO 8 hours.

source_name: Lymphoblastoid cell lines derived from GM13042

treatment_protocol: DMSO 8 hours

tag: treatment

value: DMSO


title: NB_E_11


title: erStress_131grandparents_GM13042_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM13042 treated with 4 ug/ml tunicamycin 8 hours.


title: erStress_131grandparents_GM12979_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12979 treated with 4 ug/ml tunicamycin 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12979


title: erStress_131grandparents_GM13133_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM13133 treated with DMSO 8 hours.


title: erStress_131grandparents_GM12978_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12978 treated with 4 ug/ml tunicamycin 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12978


title: NB_E_8


title: erStress_131grandparents_GM12979_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12979 treated with DMSO 8 hours.


title: NB_E_6

value: F


title: erStress_131grandparents_GM12977_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12977 treated with 4 ug/ml tunicamycin 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12977


title: erStress_131grandparents_GM12978_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12978 treated with DMSO 8 hours.


title: erStress_131grandparents_GM12977_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12977 treated with DMSO 8 hours.


title: NB_E_7


title: NB_E_3


title: erStress_131grandparents_GM12908_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12908 treated with 4 ug/ml tunicamycin 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12908


title: erStress_131grandparents_GM12921_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12921 treated with 4 ug/ml tunicamycin 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12921


title: erStress_131grandparents_GM12890_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12890 treated with 4 ug/ml tunicamycin 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12890


title: erStress_131grandparents_GM12890_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12890 treated with DMSO 8 hours.


title: NB_CL24

source_name: Cell line

extract_protocol: Cells were homogenized in TRIzol reagent (Invitrogen, Karlsruhe, Germany) using the FastPrep FP120 cell disruptor (Qbiogene, Inc, Carlsbad, CA, USA).Total RNA was isolated following the TRIzol protocol (Invitrogen) and RNA integrity was assessed using the 2100 Bioanalyzer (Agilent, Waldbronn, Germany). Only samples with an RNA Integrity Number >7.5 were taken for further analysis.

value: SK-N-AS

tag: transgene

value: GFP


title: erStress_131grandparents_GM12921_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12921 treated with DMSO 8 hours.


title: NB_E_5

tag: tissue

value: M


title: erStress_131grandparents_GM12908_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12908 treated with DMSO 8 hours.


title: NB_E_4

tag: Sex

value: <18 months; 2:>18 months):1


title: erStress_131grandparents_GM12889_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12889 treated with 4 ug/ml tunicamycin 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12889


title: NB_E_2

tag: mycn (1

value: not amplified; Amp:amplified; n.a.:not available):Amp

value: <18 months; 2:>18 months):2


title: erStress_131grandparents_GM12889_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12889 treated with DMSO 8 hours.


title: erStress_131grandparents_GM12862_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12862 treated with 4 ug/ml tunicamycin 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12862


title: erStress_131grandparents_GM12863_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12863 treated with 4 ug/ml tunicamycin 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12863


title: NB_E_1

tag: Stage


title: erStress_131grandparents_GM12863_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12863 treated with DMSO 8 hours.


title: erStress_131grandparents_GM12862_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12862 treated with DMSO 8 hours.


title: NB_CL22


title: erStress_131grandparents_GM12860_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12860 treated with DMSO 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12860


title: erStress_131grandparents_GM12861_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12861 treated with 4 ug/ml tunicamycin 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12861


title: erStress_131grandparents_GM12843_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12843 treated with DMSO 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12843


title: erStress_131grandparents_GM12861_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12861 treated with DMSO 8 hours.


title: NB_CL23


title: erStress_131grandparents_GM12860_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12860 treated with 4 ug/ml tunicamycin 8 hours.


title: erStress_131grandparents_GM12842_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12842 treated with DMSO 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12842


title: NB_CL20

value: 6 h


title: NB_CL21


title: erStress_131grandparents_GM12843_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12843 treated with 4 ug/ml tunicamycin 8 hours.


title: erStress_131grandparents_GM12830_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12830 treated with 4 ug/ml tunicamycin 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12830


title: NB_CL18


title: erStress_131grandparents_GM12840_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12840 treated with DMSO 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12840


title: erStress_131grandparents_GM12841_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12841 treated with 4 ug/ml tunicamycin 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12841


title: erStress_131grandparents_GM12842_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12842 treated with 4 ug/ml tunicamycin 8 hours.


title: erStress_131grandparents_GM12841_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12841 treated with DMSO 8 hours.


title: NB_CL19

value: 0 h


title: erStress_131grandparents_GM12829_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12829 treated with 4 ug/ml tunicamycin 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12829


title: erStress_131grandparents_GM12830_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12830 treated with DMSO 8 hours.


title: erStress_131grandparents_GM12777_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12777 treated with 4 ug/ml tunicamycin 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12777


title: erStress_131grandparents_GM12829_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12829 treated with DMSO 8 hours.


title: erStress_131grandparents_GM12778_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12778 treated with 4 ug/ml tunicamycin 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12778


title: NB_CL12


title: erStress_131grandparents_GM12840_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12840 treated with 4 ug/ml tunicamycin 8 hours.


title: erStress_131grandparents_GM12827_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12827 treated with DMSO 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12827


title: erStress_131grandparents_GM12827_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12827 treated with 4 ug/ml tunicamycin 8 hours.


title: NB_CL16


title: NB_CL10


title: NB_CL13


title: erStress_131grandparents_GM12828_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12828 treated with 4 ug/ml tunicamycin 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12828


title: erStress_131grandparents_GM12777_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12777 treated with DMSO 8 hours.


title: NB_CL14


title: NB_CL8


title: NB_CL11


title: NB_CL9


title: erStress_131grandparents_GM12828_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12828 treated with DMSO 8 hours.


title: erStress_131grandparents_GM12775_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12775 treated with DMSO 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12775


title: erStress_131grandparents_GM12776_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12776 treated with DMSO 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12776


title: erStress_131grandparents_GM12776_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12776 treated with 4 ug/ml tunicamycin 8 hours.


title: erStress_131grandparents_GM12775_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12775 treated with 4 ug/ml tunicamycin 8 hours.


title: erStress_131grandparents_GM12749_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12749 treated with DMSO 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12749


title: erStress_131grandparents_GM12749_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12749 treated with 4 ug/ml tunicamycin 8 hours.


title: erStress_131grandparents_GM12718_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12718 treated with DMSO 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12718


title: erStress_131grandparents_GM12718_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12718 treated with 4 ug/ml tunicamycin 8 hours.


title: erStress_131grandparents_GM12569_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12569 treated with DMSO 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12569


title: erStress_131grandparents_GM12558_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12558 treated with 4 ug/ml tunicamycin 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12558


title: NB_CL15

source_name: Cell line


title: erStress_131grandparents_GM12569_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12569 treated with 4 ug/ml tunicamycin 8 hours.


title: erStress_131grandparents_GM12558_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12558 treated with DMSO 8 hours.


title: erStress_131grandparents_GM12489_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12489 treated with DMSO 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12489


title: NB_CL1


title: NB_CL6


title: NB_CL5


title: NB_CL7


title: NB_CL2


title: NB_CL4


title: erStress_131grandparents_GM12489_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12489 treated with 4 ug/ml tunicamycin 8 hours.


title: erStress_131grandparents_GM12466_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12466 treated with DMSO 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12466


title: erStress_131grandparents_GM12413_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12413 treated with DMSO 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12413


title: erStress_131grandparents_GM12481_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12481 treated with 4 ug/ml tunicamycin 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12481


title: erStress_131grandparents_GM12481_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12481 treated with DMSO 8 hours.


title: erStress_131grandparents_GM12482_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12482 treated with 4 ug/ml tunicamycin 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12482


title: erStress_131grandparents_GM12466_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12466 treated with 4 ug/ml tunicamycin 8 hours.


title: erStress_131grandparents_GM12482_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12482 treated with DMSO 8 hours.


title: erStress_131grandparents_GM12398_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12398 treated with 4 ug/ml tunicamycin 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12398


title: erStress_131grandparents_GM12400_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12400 treated with 4 ug/ml tunicamycin 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12400


title: NB_CL3


title: erStress_131grandparents_GM12383_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12383 treated with DMSO 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12383


title: erStress_131grandparents_GM12341_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12341 treated with DMSO 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12341


title: erStress_131grandparents_GM12341_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12341 treated with 4 ug/ml tunicamycin 8 hours.


title: erStress_131grandparents_GM12413_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12413 treated with 4 ug/ml tunicamycin 8 hours.


title: erStress_131grandparents_GM12400_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12400 treated with DMSO 8 hours.


title: erStress_131grandparents_GM12340_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12340 treated with DMSO 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12340


title: erStress_131grandparents_GM12282_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12282 treated with 4 ug/ml tunicamycin 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12282


title: erStress_131grandparents_GM12383_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12383 treated with 4 ug/ml tunicamycin 8 hours.


title: erStress_131grandparents_GM12398_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12398 treated with DMSO 8 hours.


title: erStress_131grandparents_GM12286_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12286 treated with 4 ug/ml tunicamycin 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12286


title: erStress_131grandparents_GM12283_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12283 treated with 4 ug/ml tunicamycin 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12283


title: erStress_131grandparents_GM12282_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12282 treated with DMSO 8 hours.


title: erStress_131grandparents_GM12286_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12286 treated with DMSO 8 hours.


title: erStress_131grandparents_GM12274_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12274 treated with 4 ug/ml tunicamycin 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12274


title: erStress_131grandparents_GM12283_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12283 treated with DMSO 8 hours.


title: erStress_131grandparents_GM12340_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12340 treated with 4 ug/ml tunicamycin 8 hours.


title: NB_CL17


title: erStress_131grandparents_GM12274_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12274 treated with DMSO 8 hours.


title: erStress_131grandparents_GM12251_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12251 treated with DMSO 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12251


title: erStress_131grandparents_GM12097_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12097 treated with DMSO 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12097


title: erStress_131grandparents_GM12273_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12273 treated with 4 ug/ml tunicamycin 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12273


title: erStress_131grandparents_GM12058_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12058 treated with 4 ug/ml tunicamycin 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12058


title: erStress_131grandparents_GM12250_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12250 treated with DMSO 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12250


title: erStress_131grandparents_GM12097_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12097 treated with 4 ug/ml tunicamycin 8 hours.


title: erStress_131grandparents_GM12273_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12273 treated with DMSO 8 hours.


title: Buccal 1 rep 3

type: genomic

city: London

first: Robert

last: Lowe

address: 4 Newark Street

department: Blizard Institute

country: United Kingdom

institute: QMUL

description: Normal

hyb_protocol: Bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol

scan_protocol: Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting.

data_processing: BeadStudio software, Custom R scripts for normalisation provided in supplementary of paper.

label: Cy5 and Cy3

molecule: genomic DNA

source_name: Buccal

label_protocol: Standard Illumina Protocol

extract_protocol: DNA from Buccal cells was extracted using the Gentra Puregene Buccal cell kit, USA. CD14 and CD34 cells were extracted using Qiagen QIAamp DNA Blood Mini Kit, Sperm was extracted by Qiagen QIAamp DNA Mini Kit, Placenta (Catalog number D1234200 from Biochain, USA) and Pancreas (Catalog number D1234188 from Biochain, USA) were commercial samples

tag: gender

value: Male


title: erStress_131grandparents_GM12058_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12058 treated with DMSO 8 hours.


title: erStress_131grandparents_GM12045_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12045 treated with DMSO 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12045


title: erStress_131grandparents_GM12250_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12250 treated with 4 ug/ml tunicamycin 8 hours.


title: erStress_131grandparents_GM12045_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12045 treated with 4 ug/ml tunicamycin 8 hours.


title: erStress_131grandparents_GM12251_tunicamycin

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12251 treated with 4 ug/ml tunicamycin 8 hours.


title: erStress_131grandparents_GM12778_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM12778 treated with DMSO 8 hours.

source_name: Lymphoblastoid cell lines derived from GM12778


title: CD14 8

description: Normal


title: CD14 6

description: Normal


title: CD14 3

description: Normal


title: CD14 4

description: Normal


title: CD14 5

description: Normal


title: Buccal 1 rep 2

description: Normal


title: CD14 9

description: Normal


title: erStress_131grandparents_GM11933_dmso

description: Gene expression data under ER stress from lymphoblastoid cells of individual GM11933 treated with DMSO 8 hours.

source_name: Lymphoblastoid cell lines derived from GM11933


title: Buccal 1 rep 1

description: Normal


title: CD14 2

description: Normal