title: mms 5 min

description: mms 5 min

scan_protocol: Pixel Size : 5 Scan Date : 2008-03-06 Scan Time : 09:56:53 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: mms 5 mins

treatment_protocol:
Time : 5 minutes
Compound Based Treatment : 0.1% MMS
Growth Condition : 25C YPD


title: 240 time point cell cycle of S.B

status: Public on Jan 24 2010

type: RNA

city: Seattle

first: Maitreya

middle: J.

last: Dunham

state: WA

address: Foege Building, S403B, Box 355065

department: Genome Sciences

country: USA

institute: University of Washington

description: 240 time point cell cycle of S.B

hyb_protocol: reference URL : http://genomics.princeton.edu/caudylab/Yeast_RNA_Prep_and_Labeling_070925.pdf

scan_protocol: Scan Power : 100 PMT Volts : 522 Pixel Size : 5 Laser on time : 13204 Laser Power : 4.99 Scan Date : 2007-08-03 Scan Temperature : 29.47 Scan Time : 12:05:50 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.0.1.25
Datafile type : GenePix Results 3
Image Origin : 0, 0
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

molecule: total RNA

organism: Saccharomyces bayanus

source_name: 240 mins after alpha-factor release

extract_protocol:
Extracted molecule : RNA
Extraction method : acid phenol extraction

treatment_protocol:
Compound Based Treatment : pronase after alpha factor arrest
Growth Condition : 25C YPD
Time : 240 minutes

tag: species

value: S. bayanus

tag: strain name

value: ACY7, prototroph MATa

source_name: asynchronous reference

treatment_protocol: Growth Condition : 25C YPD asynchronous

tag: genotype

value: prototroph

tag: sex/mating type

value: MATa


title: 250 time point cell cycle of S.B

description: 250 time point cell cycle of S.B

scan_protocol: Scan Power : 100 PMT Volts : 526 Pixel Size : 5 Laser on time : 13153 Laser Power : 4.94 Scan Date : 2007-08-03 Scan Temperature : 29.13 Scan Time : 11:14:28 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

source_name: 250 mins after alpha-factor release

label_protocol: reference URL : http://genomics.princeton.edu/caudylab/Yeast_RNA_Prep_and_Labeling_070925.pdf

treatment_protocol:
Compound Based Treatment : pronase after alpha factor arrest
Growth Condition : 25C YPD
Time : 250 minutes


title: time point cell cycle of S.B -2 270 min

description: time point cell cycle of S.B -2 270 min

scan_protocol: Scan Power : 100 PMT Volts : 505 Pixel Size : 5 Laser on time : 13876 Laser Power : 5.29 Scan Date : 2007-08-08 Scan Temperature : 28.89 Scan Time : 10:16:56 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

source_name: 270 mins after alpha-factor release

treatment_protocol:
Compound Based Treatment : pronase after alpha factor arrest
Growth Condition : 25C YPD
Time : 270 minutes


title: 230 time point cell cycle of S.B

description: 230 time point cell cycle of S.B

scan_protocol: Scan Power : 100 PMT Volts : 521 Pixel Size : 5 Laser on time : 13172 Laser Power : 4.96 Scan Date : 2007-08-03 Scan Temperature : 29.47 Scan Time : 11:33:35 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

source_name: 230 mins after alpha-factor release

treatment_protocol:
Compound Based Treatment : pronase after alpha factor arrest
Growth Condition : 25C YPD
Time : 230 minutes


title: NRRL-Y11845 vs. CY1 log growth

description: NRRL-Y11845 vs. Reference CY1 same norm with Sb only

scan_protocol: Pixel Size : 5 Scan Date : 2007-06-28 Scan Time : 12:56:29 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 5.1.0.10
Datafile type : GenePix Results 3
Image Origin : 0, 0
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

source_name: CY1 grown to log phase

treatment_protocol: Growth Condition : 25C YNB, midlog

source_name: NRRL-Y11845 grown to log phase

value: MATa/alpha


title: sorbitol 15 mins

description: sorbitol 15 mins

scan_protocol: Pixel Size : 5 Scan Date : 2008-03-06 Scan Time : 11:05:04 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.4
Datafile type : GenePix Results 3
Image Origin : 0, 0
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

source_name: reference

treatment_protocol: Growth Condition : mixed

value: mixed

source_name: sorbitol 15 mins

treatment_protocol:
Time : 15 minutes
Compound Based Treatment : 1 M sorbitol
Growth Condition : 25C YPD

value: MATalpha


title: sorbitol 30 mins

description: sorbitol 30 mins

scan_protocol: Pixel Size : 5 Scan Date : 2008-03-06 Scan Time : 11:21:38 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: sorbital 30 mins

treatment_protocol:
Time : 30 minutes
Compound Based Treatment : 1 M sorbitol
Growth Condition : 25C YPD


title: sorbitol 5 mins

description: sorbitol 5 mins

scan_protocol: Pixel Size : 5 Scan Date : 2008-03-06 Scan Time : 10:48:39 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: sorbitol 5 mins

treatment_protocol:
Time : 5 minutes
Compound Based Treatment : 1 M sorbitol
Growth Condition : 25C YPD


title: DBY10075 vs. CY1 log growth

description: DBY10075 vs. Reference CY1 same norm with Sb only

scan_protocol: Pixel Size : 5 Scan Date : 2007-06-28 Scan Time : 12:37:47 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: DBY10075 grown to log phase


title: sorbitol 10 mins

description: sorbitol 10 mins

scan_protocol: Pixel Size : 5 Scan Date : 2008-03-06 Scan Time : 10:56:51 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: sorbitol 10 mins

treatment_protocol:
Time : 10 minutes
Compound Based Treatment : 1 M sorbitol
Growth Condition : 25C YPD


title: sorbitol 20 mins

description: sorbitol 20 mins

scan_protocol: Pixel Size : 5 Scan Date : 2008-03-06 Scan Time : 11:13:25 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: sorbitol 20 mins

treatment_protocol:
Time : 20 minutes
Compound Based Treatment : 1 M sorbitol
Growth Condition : 25C YPD


title: sorbitol 0 mins

description: sorbitol 0 mins

scan_protocol: Pixel Size : 5 Scan Date : 2008-03-06 Scan Time : 10:40:27 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: sorbitol 0 min

treatment_protocol:
Time : 0 minutes
Compound Based Treatment : 1 M sorbitol
Growth Condition : 25C YPD


title: time point cell cycle of S.B -1 260 min

description: time point cell cycle of S.B -1 260 min

scan_protocol: Scan Power : 100 PMT Volts : 495 Pixel Size : 5 Laser on time : 13855 Laser Power : 5.26 Scan Date : 2007-08-08 Scan Temperature : 28.80 Scan Time : 09:55:40 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

source_name: 260 mins after alpha-factor release

treatment_protocol:
Compound Based Treatment : pronase after alpha factor arrest
Growth Condition : 25C YPD
Time : 260 minutes


title: CY1 vs. CY1 log growth

description: Reference CY1 vs. Reference CY1 same norm with Sb only

scan_protocol: Pixel Size : 5 Scan Date : 2007-06-28 Scan Time : 12:27:18 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1


title: mms 10 min

description: mms 10 min

scan_protocol: Pixel Size : 5 Scan Date : 2008-03-06 Scan Time : 10:05:05 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: mms 10 mins

treatment_protocol:
Time : 10 minutes
Compound Based Treatment : 0.1% MMS
Growth Condition : 25C YPD


title: 200 time point cell cycle of S.B

description: 200 time point cell cycle of S.B

scan_protocol: Scan Power : 100 PMT Volts : 539 Pixel Size : 5 Laser on time : 12559 Laser Power : 4.60 Scan Date : 2007-07-31 Scan Temperature : 27.61 Scan Time : 13:03:25 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.2
Datafile type : GenePix Results 3
Image Origin : 0, 0
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

source_name: 200 mins after alpha-factor release

treatment_protocol:
Compound Based Treatment : pronase after alpha factor arrest
Growth Condition : 25C YPD
Time : 200 minutes


title: 180 time point cell cycle of S.B

description: 180 time point cell cycle of S.B

scan_protocol: Scan Power : 100 PMT Volts : 545 Pixel Size : 5 Laser on time : 12617 Laser Power : 4.66 Scan Date : 2007-07-31 Scan Temperature : 28.65 Scan Time : 14:01:07 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

source_name: 180 mins after alpha-factor release

treatment_protocol:
Compound Based Treatment : pronase after alpha factor arrest
Growth Condition : 25C YPD
Time : 180 minutes

source_name: asynchronous reference


title: 220 time point cell cycle of S.B

description: 220 time point cell cycle of S.B

scan_protocol: Scan Power : 100 PMT Volts : 506 Pixel Size : 5 Laser on time : 13089 Laser Power : 4.88 Scan Date : 2007-08-03 Scan Temperature : 28.68 Scan Time : 10:10:09 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

source_name: 220 mins after alpha-factor release

treatment_protocol:
Compound Based Treatment : pronase after alpha factor arrest
Growth Condition : 25C YPD
Time : 220 minutes


title: 170 time point cell cycle of S.B

description: 170 time point cell cycle of S.B

scan_protocol: Scan Power : 100 PMT Volts : 525 Pixel Size : 5 Laser on time : 12638 Laser Power : 4.67 Scan Date : 2007-07-31 Scan Temperature : 29.17 Scan Time : 14:22:21 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

source_name: 170 mins after alpha-factor release

treatment_protocol:
Compound Based Treatment : pronase after alpha factor arrest
Growth Condition : 25C YPD
Time : 170 minutes

treatment_protocol: Growth Condition : 25C YPD asynchronous


title: 210 time point cell cycle of S.B

description: 210 time point cell cycle of S.B

scan_protocol: Scan Power : 100 PMT Volts : 525 Pixel Size : 5 Laser on time : 13128 Laser Power : 4.92 Scan Date : 2007-08-03 Scan Temperature : 28.77 Scan Time : 10:49:00 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

source_name: 210 mins after alpha-factor release

treatment_protocol:
Compound Based Treatment : pronase after alpha factor arrest
Growth Condition : 25C YPD
Time : 210 minutes


title: 150 time point cell cycle of S.B fixed

description: 150 time point cell cycle of S.B fixed

scan_protocol: Scan Power : 100 PMT Volts : 615 Pixel Size : 5 Laser on time : 12416 Laser Power : 4.58 Scan Date : 2007-07-24 Scan Temperature : 29.20 Scan Time : 13:39:10 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

source_name: 150 mins after alpha-factor release

treatment_protocol:
Compound Based Treatment : pronase after alpha factor arrest
Growth Condition : 25C YPD
Time : 150 minutes


title: 160 time point cell cycle of S.B fixed

description: 160 time point cell cycle of S.B fixed

scan_protocol: Scan Power : 100 PMT Volts : 592 Pixel Size : 5 Laser on time : 12435 Laser Power : 4.62 Scan Date : 2007-07-24 Scan Temperature : 29.26 Scan Time : 13:57:56 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

source_name: 160 mins after alpha-factor release

treatment_protocol:
Compound Based Treatment : pronase after alpha factor arrest
Growth Condition : 25C YPD
Time : 160 minutes


title: BB11 vs. CY1 log growth

description: BB11 vs. Reference CY1

scan_protocol: Pixel Size : 5 Scan Date : 2007-06-28 Scan Time : 13:06:59 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: CY1 grown to log phase

treatment_protocol: Growth Condition : 25C YNB, midlog

source_name: BB11 grown to log phase


title: 190 time point cell cycle of S.B

description: 190 time point cell cycle of S.B

scan_protocol: Scan Power : 100 PMT Volts : 560 Pixel Size : 5 Laser on time : 12597 Laser Power : 4.65 Scan Date : 2007-07-31 Scan Temperature : 28.40 Scan Time : 13:41:25 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

source_name: 190 mins after alpha-factor release

treatment_protocol:
Compound Based Treatment : pronase after alpha factor arrest
Growth Condition : 25C YPD
Time : 190 minutes


title: 130 time point cell cycle of S.B f

description: 130 time point cell cycle of S.B f

scan_protocol: Scan Power : 100 PMT Volts : 592 Pixel Size : 5 Laser on time : 15373 Laser Power : 5.95 Scan Date : 2007-08-15 Scan Temperature : 27.55 Scan Time : 10:12:09 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

source_name: 130 mins after alpha-factor release

treatment_protocol:
Compound Based Treatment : pronase after alpha factor arrest
Growth Condition : 25C YPD
Time : 130 minutes


title: Alpha- factor II- 120 min

description: Alpha- factor II- 120 min

scan_protocol: Scan Power : 100 PMT Volts : 505 Pixel Size : 5 Laser on time : 12807 Laser Power : 4.80 Scan Date : 2007-07-31 Scan Temperature : 30.42 Scan Time : 17:16:55 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

source_name: Mixed reference

source_name: 120 min

treatment_protocol:
Time : 120 minutes
Compound Based Treatment : 50 ng/mL alpha factor
Growth Condition : 25C YPD


title: 0 time point cell cycle of S.B n

description: 0 time point cell cycle of S.B n

scan_protocol: Scan Power : 100 PMT Volts : 521 Pixel Size : 5 Laser on time : 16184 Laser Power : 6.49 Scan Date : 2007-08-23 Scan Temperature : 24.47 Scan Time : 10:19:04 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

source_name: 0 min after alpha-factor release n

treatment_protocol:
Compound Based Treatment : pronase after alpha factor arrest
Growth Condition : 25C YPD
Time : 0 minutes


title: Alpha- factor II- 90 min

description: Alpha- factor II- 90 min

scan_protocol: Scan Power : 100 PMT Volts : 520 Pixel Size : 5 Laser on time : 12788 Laser Power : 4.81 Scan Date : 2007-07-31 Scan Temperature : 30.11 Scan Time : 16:57:37 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

treatment_protocol: Growth Condition : mixed

source_name: 90 min

treatment_protocol:
Time : 90 minutes
Compound Based Treatment : 50 ng/mL alpha factor
Growth Condition : 25C YPD


title: 140 time point cell cycle of S.B fixed

description: 140 time point cell cycle of S.B fixed

scan_protocol: Scan Power : 100 PMT Volts : 575 Pixel Size : 5 Laser on time : 12331 Laser Power : 4.54 Scan Date : 2007-07-24 Scan Temperature : 27.82 Scan Time : 12:14:01 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

source_name: 140 mins after alpha-factor release

treatment_protocol:
Compound Based Treatment : pronase after alpha factor arrest
Growth Condition : 25C YPD
Time : 140 minutes


title: Alpha- factor II- 60 min

description: Alpha- factor II- 60 min

scan_protocol: Scan Power : 100 PMT Volts : 522 Pixel Size : 5 Laser on time : 12769 Laser Power : 4.80 Scan Date : 2007-07-31 Scan Temperature : 29.96 Scan Time : 16:39:14 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

source_name: 60 min

treatment_protocol:
Time : 60 minutes
Compound Based Treatment : 50 ng/mL alpha factor
Growth Condition : 25C YPD


title: 110 time point cell cycle of S.B

description: 110 time point cell cycle of S.B

scan_protocol: Scan Power : 100 PMT Volts : 529 Pixel Size : 5 Laser on time : 14247 Laser Power : 5.52 Scan Date : 2007-08-10 Scan Temperature : 29.10 Scan Time : 11:03:42 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

source_name: 110 mins after alpha-factor release

treatment_protocol:
Compound Based Treatment : pronase after alpha factor arrest
Growth Condition : 25C YPD
Time : 110 minutes


title: 120 time point cell cycle of S.B fixed

description: 120 time point cell cycle of S.B fixed

scan_protocol: Scan Power : 100 PMT Volts : 525 Pixel Size : 5 Laser on time : 14273 Laser Power : 5.55 Scan Date : 2007-08-10 Scan Temperature : 29.44 Scan Time : 11:29:54 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

source_name: 120 mins after alpha-factor release

treatment_protocol:
Compound Based Treatment : pronase after alpha factor arrest
Growth Condition : 25C YPD
Time : 120 minutes

source_name: asynchronous reference


title: Alpha- factor II- 45 min

description: Alpha- factor II- 45 min

scan_protocol: Scan Power : 100 PMT Volts : 509 Pixel Size : 5 Laser on time : 12751 Laser Power : 4.79 Scan Date : 2007-07-31 Scan Temperature : 30.02 Scan Time : 16:20:50 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

source_name: 45 min

treatment_protocol:
Time : 45 minutes
Compound Based Treatment : 50 ng/mL alpha factor
Growth Condition : 25C YPD


title: 90 time point cell cycle of S.B

description: 90 time point cell cycle of S.B

scan_protocol: Scan Power : 100 PMT Volts : 556 Pixel Size : 5 Laser on time : 11921 Laser Power : 4.53 Scan Date : 2007-07-13 Scan Temperature : 30.17 Scan Time : 14:08:39 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

source_name: 90 mins after alpha-factor release

treatment_protocol:
Compound Based Treatment : pronase after alpha factor arrest
Growth Condition : 25C YPD
Time : 90 minutes


title: 70 time point cell cycle of S.B n

description: 70 time point cell cycle of S.B n

scan_protocol: Pixel Size : 5 Scan Date : 2007-07-10 Scan Time : 10:28:33 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: 70 mins after alpha-factor release

treatment_protocol:
Compound Based Treatment : pronase after alpha factor arrest
Growth Condition : 25C YPD
Time : 70 minutes

treatment_protocol: Growth Condition : 25C YPD asynchronous


title: 100 time point cell cycle of S.B

description: 100 time point cell cycle of S.B

scan_protocol: Scan Power : 100 PMT Volts : 600 Pixel Size : 5 Laser on time : 9923 Laser Power : 3.82 Scan Date : 2007-06-19 Scan Temperature : 26.78 Scan Time : 12:25:59 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

source_name: 100 mins after alpha-factor release

treatment_protocol:
Compound Based Treatment : pronase after alpha factor arrest
Growth Condition : 25C YPD
Time : 100 minutes


title: 80 time point cell cycle of S.B

description: 80 time point cell cycle of S.B

scan_protocol: Scan Power : 100 PMT Volts : 534 Pixel Size : 5 Laser on time : 11788 Laser Power : 4.42 Scan Date : 2007-07-13 Scan Temperature : 29.59 Scan Time : 11:55:22 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

source_name: 80 mins after alpha-factor release

treatment_protocol:
Compound Based Treatment : pronase after alpha factor arrest
Growth Condition : 25C YPD
Time : 80 minutes


title: 60 time point cell cycle of S.B n

description: 60 time point cell cycle of S.B n

scan_protocol: Pixel Size : 5 Scan Date : 2007-07-10 Scan Time : 10:45:12 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: 60 mins after alpha-factor release

treatment_protocol:
Compound Based Treatment : pronase after alpha factor arrest
Growth Condition : 25C YPD
Time : 60 minutes


title: Alpha- factor II- 0 min

description: Alpha- factor II- 0 min

scan_protocol: Scan Power : 100 PMT Volts : 509 Pixel Size : 5 Laser on time : 12677 Laser Power : 4.71 Scan Date : 2007-07-31 Scan Temperature : 29.41 Scan Time : 15:06:57 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

source_name: Mixed Reference

source_name: 0 min

treatment_protocol:
Time : 0 minutes
Compound Based Treatment : 50 ng/mL alpha factor
Growth Condition : 25C YPD


title: 50 time point cell cycle of S.B n

description: 50 time point cell cycle of S.B n

scan_protocol: Pixel Size : 5 Scan Date : 2007-07-10 Scan Time : 10:19:32 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: 50 mins after alpha-factor release

treatment_protocol:
Compound Based Treatment : pronase after alpha factor arrest
Growth Condition : 25C YPD
Time : 50 minutes


title: 2-deoxyglucose 20 min

description: cerevisiae exposed to drug for 20 min

scan_protocol: Pixel Size : 5 Scan Date : 2007-09-27 Scan Time : 13:45:05 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: reference

source_name: sample

treatment_protocol:
Time : 20 minutes
Compound Based Treatment : 2 mM 2-deoxy-D-glucose
Growth Condition : 25C YPD


title: 2-deoxyglucose 15 min. Again

description: 2-deoxyglucose 15 min. Again

scan_protocol: Scan Power : 100 PMT Volts : 465 Pixel Size : 5 Laser on time : 16696 Laser Power : 3.87 Scan Date : 2007-09-27 Scan Temperature : 29.87 Scan Time : 16:13:36 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.4
Datafile type : GenePix Results 3
Image Origin : 440, 4040
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

source_name: 2-deoxyglucose 15 min.

treatment_protocol:
Compound Based Treatment : 2 mM 2-deoxy-D-glucose
Growth Condition : 25C YPD
Time : 15 minutes

value: ACY12, prototroph MATalpha


title: 2-deoxyglucose 5 min

description: 5 minute timepoint, after adding 2-deoxyglucose

scan_protocol: Pixel Size : 5 Scan Date : 2007-09-27 Scan Time : 15:46:30 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: Reference

treatment_protocol: Growth Condition : mixed

source_name: Treatment 5 min

treatment_protocol:
Time : 5 minutes
Compound Based Treatment : 2 mM 2-deoxy-D-glucose
Growth Condition : 25C YPD


title: 2-deoxyglucose 10 min

description: 10 min timepoint, after adding 2-deoxyglucose

scan_protocol: Pixel Size : 5 Scan Date : 2007-09-27 Scan Time : 12:59:25 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: Treatment 10 min

treatment_protocol:
Time : 10 minutes
Compound Based Treatment : 2 mM 2-deoxy-D-glucose
Growth Condition : 25C YPD


title: 20 time point cell cycle of S.B

description: 20 time point cell cycle of S.B

scan_protocol: Scan Power : 100 PMT Volts : 600 Pixel Size : 5 Laser on time : 10109 Laser Power : 3.91 Scan Date : 2007-06-21 Scan Temperature : 28.43 Scan Time : 11:00:38 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

source_name: 20 mins after alpha-factor release

treatment_protocol:
Compound Based Treatment : pronase after alpha factor arrest
Growth Condition : 25C YPD
Time : 20 minutes


title: 10 time point cell cycle of S.B n

description: 10 time point cell cycle of S.B n

scan_protocol: Pixel Size : 5 Scan Date : 2007-07-10 Scan Time : 10:53:43 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: 10 mins after alpha-factor release

treatment_protocol:
Compound Based Treatment : pronase after alpha factor arrest
Growth Condition : 25C YPD
Time : 10 minutes

source_name: asynchronous reference


title: Alpha- factor II- 5 min

description: Alpha- factor II- 5 min

scan_protocol: Scan Power : 100 PMT Volts : 496 Pixel Size : 5 Laser on time : 12695 Laser Power : 4.73 Scan Date : 2007-07-31 Scan Temperature : 29.44 Scan Time : 15:24:48 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

source_name: Mixed reference

source_name: 5 min

treatment_protocol:
Time : 5 minutes
Compound Based Treatment : 50 ng/mL alpha factor
Growth Condition : 25C YPD


title: Alpha- factor II- 15 min

description: Alpha- factor II- 15 min

scan_protocol: Scan Power : 100 PMT Volts : 505 Pixel Size : 5 Laser on time : 12713 Laser Power : 4.75 Scan Date : 2007-07-31 Scan Temperature : 29.68 Scan Time : 15:42:50 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

source_name: 15 min

treatment_protocol:
Time : 15 minutes
Compound Based Treatment : 50 ng/mL alpha factor
Growth Condition : 25C YPD


title: 2-deoxyglucose 0 min

description: Zero timepoint, before adding 2-deoxyglucose

scan_protocol: Pixel Size : 5 Scan Date : 2007-09-27 Scan Time : 13:26:45 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: Treatment 0 min

treatment_protocol:
Time : 0 minutes
Compound Based Treatment : 2 mM 2-deoxy-D-glucose
Growth Condition : 25C YPD


title: a mating type bayanus

description: no change in norm with Sb only

scan_protocol: Pixel Size : 5 Scan Date : 2007-07-27 Scan Time : 20:23:27 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: BB11 grown to log phase

treatment_protocol: Growth Condition : 25C YPD midlog

source_name: a mating type bayanus

treatment_protocol: Growth Condition : 25C chemostat media + 0.24% glucose, midlog

value: BB11 MATa derivative


title: LovastatinT20

description: S. bayanus. Drug: Lovastatin. 20 min.

scan_protocol: Pixel Size : 5 Scan Date : 2007-09-27 Scan Time : 12:33:33 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: mixed reference S.  cerevisiae

source_name: S. bayanus

treatment_protocol:
Time : 20 minutes
Compound Based Treatment : 43 ug/mL lovastatin
Growth Condition : 25C YPD


title: Diauxic Shift 6

description: no change in norm with Sb only

scan_protocol: Pixel Size : 5 Scan Date : 2007-07-17 Scan Time : 16:25:51 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: BB11 in log phase growth

source_name: BB11 at 5:15 PM

treatment_protocol:
Time : 15.5 hours
Growth Condition : 25C chemostat media + 0.24% glucose


title: 40 time point cell cycle of S.B fixed

description: 40 time point cell cycle of S.B fixed

scan_protocol: Scan Power : 100 PMT Volts : 566 Pixel Size : 5 Laser on time : 12310 Laser Power : 4.45 Scan Date : 2007-07-24 Scan Temperature : 26.88 Scan Time : 11:53:21 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

source_name: 40 mins after alpha-factor release

treatment_protocol:
Compound Based Treatment : pronase after alpha factor arrest
Growth Condition : 25C YPD
Time : 40 minutes

treatment_protocol: Growth Condition : 25C YPD asynchronous


title: Diauxic Shift 5

description: no change in norm with Sb only

scan_protocol: Pixel Size : 5 Scan Date : 2007-07-17 Scan Time : 16:35:14 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: BB11 at 4:15 PM

treatment_protocol:
Time : 14.5 hours
Growth Condition : 25C chemostat media + 0.24% glucose


title: BB11 (diploid) bayanus

description: BB11 (diploid) bayanus

scan_protocol: Pixel Size : 5 Scan Date : 2007-07-27 Scan Time : 21:38:40 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: BB11 (diploid) bayanus


title: Timepoint 30min after treatment with Tunicamycin (2mg/mL in DMSD)

scan_protocol: Pixel Size : 5 Scan Date : 2007-09-27 Scan Time : 15:11:29 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: reference

source_name: Timepoint 30min after drug treatment

treatment_protocol:
Time : 30 minutes
Compound Based Treatment : 0.002% tunicamycin
Growth Condition : 25C YPD


title: Alpha- factor II- 30 min

description: Alpha- factor II- 30 min

scan_protocol: Scan Power : 100 PMT Volts : 517 Pixel Size : 5 Laser on time : 12732 Laser Power : 4.77 Scan Date : 2007-07-31 Scan Temperature : 30.02 Scan Time : 16:02:13 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

source_name: 30 min

treatment_protocol:
Time : 30 minutes
Compound Based Treatment : 50 ng/mL alpha factor
Growth Condition : 25C YPD


title: Lovastatin Time Series - 5 Minutes

description: Other Time Points 0, 5, 10, 15 and 30 minutes

scan_protocol: Pixel Size : 5 Scan Date : 2007-09-27 Scan Time : 13:07:50 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: Untreated Reference

treatment_protocol: Growth Condition : mixed

source_name: 5 Minutes Lovastatin

treatment_protocol:
Time : 5 minutes
Compound Based Treatment : 43 ug/mL lovastatin
Growth Condition : 25C YPD


title: Diauxic Shift 3

description: norm same with Sb only

scan_protocol: Pixel Size : 5 Scan Date : 2007-07-17 Scan Time : 16:01:13 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: BB11 at 2:15 PM

treatment_protocol:
Time : 12.5 hours
Growth Condition : 25C chemostat media + 0.24% glucose


title: Timepoint 20min after treatment with Tunicamycin (2mg/mL in DMSD)

scan_protocol: Scan Power : 100 PMT Volts : 414 Pixel Size : 5 Laser on time : 42937 Laser Power : 2.28 Scan Date : 2007-09-27 Scan Temperature : 25.38 Scan Time : 15:47:35 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4200A 01  [111967] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.4
Datafile type : GenePix Results 3
Image Origin : 840, 4000
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

source_name: Reference

source_name: Timepoint 20 min

treatment_protocol:
Time : 20 minutes
Compound Based Treatment : 0.002% tunicamycin
Growth Condition : 25C YPD


title: alpha mating type bayanus

description: no change in normalizing with just Sb genes

scan_protocol: Pixel Size : 5 Scan Date : 2007-07-27 Scan Time : 21:08:16 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: BB11 grown to log phase

source_name: alpha mating type bayanus

value: BB11 MATalpha derivative


title: 2-deoxy-glucose, 30 min.

description: 2-deoxy-glucose, 30 min.

scan_protocol: Pixel Size : 5 Scan Date : 2007-09-27 Scan Time : 15:38:03 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: Mixed Reference

source_name: 2-deoxy-glucose, 30 min.

treatment_protocol:
Time : 30 minutes
Compound Based Treatment : 2 mM 2-deoxy-D-glucose
Growth Condition : 25C YPD


title: Diauxic Shift 2

description: Diauxic Shift 2

scan_protocol: Pixel Size : 5 Scan Date : 2007-07-17 Scan Time : 15:51:08 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: BB11 in log phase growth

source_name: BB11 at 1:15 PM

treatment_protocol:
Time : 11.5 hours
Growth Condition : 25C chemostat media + 0.24% glucose


title: Diauxic Shift 4

description: Diauxic Shift 4

scan_protocol: Pixel Size : 5 Scan Date : 2007-07-17 Scan Time : 16:45:55 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: BB11 at 3:15 PM

treatment_protocol:
Time : 13.5 hours
Growth Condition : 25C chemostat media + 0.24% glucose


title: 15 minute hydroxyurea

description: Time series experiment, stressing yeast with hydroxyurea, then taking time points at 0, 5, 10, 15, 20, and 30 minutes

scan_protocol: Pixel Size : 5 Scan Date : 2007-09-27 Scan Time : 15:19:58 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: Reference pool

source_name: 15 minute sample

treatment_protocol:
Time : 15 minutes
Compound Based Treatment : 50 mM hydroxyurea
Growth Condition : 25C YPD


title: Diauxic Shift 1

description: same norm with Sb only

scan_protocol: Pixel Size : 5 Scan Date : 2007-07-17 Scan Time : 15:41:48 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

treatment_protocol: Growth Condition : 25C YPD midlog

treatment_protocol:
Time : 10 hours
Growth Condition : 25C chemostat media + 0.24% glucose


title: Hydroxyurea (20 minute)

description: Hydroxyurea stress experiment in S. cerevisiae. Samples taken at 0, 5, 10, 15, 20, and 30 minutes.

scan_protocol: Scan Power : 100 PMT Volts : 580 Pixel Size : 10 Laser on time : 16498 Laser Power : 6.74 Scan Date : 2007-09-27 Scan Temperature : 26.33 Scan Time : 12:55:09 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.4
Datafile type : GenePix Results 3
Image Origin : 400, 3600
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

source_name: 20 minute sample

treatment_protocol:
Time : 20 minutes
Compound Based Treatment : 50 mM hydroxyurea
Growth Condition : 25C YPD


title: S. bayanus Tn Insert 9 vs. Mixed Reference

description: S. bayanus Tn Insert 9 grown to log phase

scan_protocol: Pixel Size : 5 Scan Date : 2007-12-15 Scan Time : 19:00:50 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: Mixed S. bayanus reference

source_name: S. bayanus Tn Insert 9 at log phase

value: Tn7 insert 9


title: Hydroxyurea (30 minute)

description: Hydroxyurea stress experiment in S. cerevisiae. Time points taked at 0, 5, 10, 15, 20, and 30 minutes.

scan_protocol: Pixel Size : 5 Scan Date : 2007-09-27 Scan Time : 12:50:23 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: 30 minute sample

treatment_protocol:
Time : 30 minutes
Compound Based Treatment : 50 mM hydroxyurea
Growth Condition : 25C YPD


title: Tunicamycin Treatment TimePoints t=10

description: Tunicamycin timepoint treatment

scan_protocol: Pixel Size : 5 Scan Date : 2007-09-27 Scan Time : 13:17:26 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: reference

source_name: drug timepoint t=10min

treatment_protocol:
Time : 10 minutes
Compound Based Treatment : 0.002% tunicamycin
Growth Condition : 25C YPD


title: Hydroxyurea (10 minute)

description: Hydroxyurea stress experiment on S. bayanus.

scan_protocol: Pixel Size : 5 Scan Date : 2007-09-27 Scan Time : 15:29:35 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: 10 minute sample

treatment_protocol:
Time : 10 minutes
Compound Based Treatment : 50 mM hydroxyurea
Growth Condition : 25C YPD


title: S. bayanus Tn insert 10 vs. Mixed Reference

description: S. bayanus Tn Insert 10 grown to log phase

scan_protocol: Pixel Size : 5 Scan Date : 2007-12-15 Scan Time : 20:11:07 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: S. bayanus Tn Insert 10 at log phase

value: Tn7 insert 10


title: Lovastatin Time Series - 30 Minutes

description: Other Time Points include 0, 5, 10, 15, 30 minutes

scan_protocol: Pixel Size : 5 Scan Date : 2007-09-27 Scan Time : 12:23:55 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: Untreated Reference

source_name: 30 Minutes Lovastatin

treatment_protocol:
Time : 30 minutes
Compound Based Treatment : 43 ug/mL lovastatin
Growth Condition : 25C YPD


title: tunicamycin treatment (t=0)

description: S.cerevisiae culture was treated with tunicamycin, a glycolyzation inhibitor. microarray assays were conducted at timepoints t=0,5,10,15,20,30. Sample is at t=0.

scan_protocol: Scan Power : 100 PMT Volts : 507 Pixel Size : 10 Laser on time : 16479 Laser Power : 6.68 Scan Date : 2007-09-27 Scan Temperature : 25.44 Scan Time : 12:37:00 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

source_name: tunicamycin treatment sample t=0

treatment_protocol:
Time : 0 minutes
Compound Based Treatment : 0.002% tunicamycin
Growth Condition : 25C YPD


title: S. bayanus Tn insert 34 vs. Mixed Reference

description: S. bayanus Tn insert 34

scan_protocol: Pixel Size : 5 Scan Date : 2008-01-04 Scan Time : 16:56:31 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: S. bayanus Tn insert 34

value: Tn7 insert 34


title: 0 minute hydroxyurea

description: RNA expression pattern for 0 minute time point in a series of 1, 5, 10, 15, 20, 30 mins in response to the addition of hydroxyurea

scan_protocol: Pixel Size : 5 Scan Date : 2007-09-27 Scan Time : 12:41:57 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: mixed reference

source_name: hydroxyurea 0 min (red)

treatment_protocol:
Time : 0 minutes
Compound Based Treatment : 50 mM hydroxyurea
Growth Condition : 25C YPD


title: Hydroxyurea (5 minutes)

description: Time point drawn from hydroxyurea stress experiment with S. bayanus. Time points drawn at 0, 5, 10, 15, 20, and 30 minutes.

scan_protocol: Scan Power : 100 PMT Volts : 551 Pixel Size : 5 Laser on time : 16601 Laser Power : 6.98 Scan Date : 2007-09-27 Scan Temperature : 29.04 Scan Time : 14:39:07 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.4
Datafile type : GenePix Results 3
Image Origin : 0, 2960
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

treatment_protocol: Growth Condition : mixed

source_name: 5 minute sample

treatment_protocol:
Time : 5 minutes
Compound Based Treatment : 50 mM hydroxyurea
Growth Condition : 25C YPD


title: Tunicamycin treatment (t = 5 min)

description: tunicamycin treatment of S. bayanus, 2mg/mL in DMSD. t = 5 min (2nd of 6 samples: t = 0, 5, 10, 15, 20, 30)

scan_protocol: Pixel Size : 5 Scan Date : 2007-09-27 Scan Time : 13:35:24 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: sample t = 5 min

treatment_protocol:
Time : 5 minutes
Compound Based Treatment : 0.002% tunicamycin
Growth Condition : 25C YPD


title: S. bayanus Tn insert 11 vs. Mixed Reference

description: S. bayanus Tn Insert 11 grown to log phase

scan_protocol: Pixel Size : 5 Scan Date : 2007-12-15 Scan Time : 18:35:18 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: S. bayanus Tn Insert 11 at log phase

value: Tn7 insert 11


title: Bleach Exposure (.25%, 20 min.)

description: Bleach Exposure (.25%, 20 min.)

scan_protocol: Scan Power : 100 PMT Volts : 522 Pixel Size : 5 Laser on time : 19919 Laser Power : 9.62 Scan Date : 2007-11-30 Scan Temperature : 30.45 Scan Time : 20:07:21 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.4
Datafile type : GenePix Results 3
Image Origin : 1640, 3040
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

source_name: Mixed Reference

source_name: Bleach Exposed

treatment_protocol:
Time : 20 minutes
Compound Based Treatment : 0.25% Bleach (NaOCl)
Growth Condition : 25C YPD


title: S. bayanus Tn Insert 3 vs. Mixed Reference

description: S. bayanus Tn Insert 3 grown to log phase,

scan_protocol: Pixel Size : 5 Scan Date : 2007-12-15 Scan Time : 20:27:30 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: Mixed S. bayanus reference

source_name: S. bayanus Tn Insert 3 at log phase

value: Tn7 insert 3


title: B25 bayanus grown at 25 C

description: B25 bayanus grown at 25 C

scan_protocol: Pixel Size : 5 Scan Date : 2007-07-27 Scan Time : 21:27:12 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: BB11 grown to log phase

source_name: bayanus grown at 25 C

treatment_protocol: Growth Condition : 25C chemostat media + 0.24% glucose, midlog


title: Tunicamycin Treatment time t=15

description: drug treatment  edited 3/4/2009: changed to swap

scan_protocol: Pixel Size : 5 Scan Date : 2007-09-27 Scan Time : 14:53:36 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: drug timepoint t=15 min

treatment_protocol:
Compound Based Treatment : 0.002% tunicamycin
Growth Condition : 25C YPD
Time : 15 minutes

source_name: reference


title: Bleach Exposure (.25%, 10 min.)

description: Bleach Exposure (.25%, 10 min.)

scan_protocol: Scan Power : 100 PMT Volts : 532 Pixel Size : 5 Laser on time : 19897 Laser Power : 9.63 Scan Date : 2007-11-30 Scan Temperature : 30.26 Scan Time : 19:46:35 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.4
Datafile type : GenePix Results 3
Image Origin : 1120, 3200
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

treatment_protocol:
Time : 10 minutes
Compound Based Treatment : 0.25% Bleach (NaOCl)
Growth Condition : 25C YPD


title: S. bayanus Tn Insert 1 vs. Mixed Reference

description: S. bayanus Tn Insert 1 grown to log phase,

scan_protocol: Pixel Size : 5 Scan Date : 2007-12-15 Scan Time : 18:52:38 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: S. bayanus Tn Insert 1 at log phase

value: Tn7 insert 1


title: B20 bayanus grown at 20 C

description: same norm with Sb only

scan_protocol: Pixel Size : 5 Scan Date : 2007-07-27 Scan Time : 20:52:36 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: bayanus grown at 20 C

treatment_protocol: Growth Condition : 20C chemostat media + 0.24% glucose, midlog


title: Bleach Exposure (.25%, 0 min.)

description: Bleach Exposure (.25%, 0 min.)

scan_protocol: Scan Power : 100 PMT Volts : 498 Pixel Size : 5 Laser on time : 19856 Laser Power : 9.46 Scan Date : 2007-11-30 Scan Temperature : 29.56 Scan Time : 19:04:02 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.4
Datafile type : GenePix Results 3
Image Origin : 800, 3200
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

treatment_protocol:
Time : 0 minutes
Compound Based Treatment : 0.25% Bleach (NaOCl)
Growth Condition : 25C YPD


title: B30 bayanus grown at 30 C

description: B30 bayanus grown at 30 C

scan_protocol: Pixel Size : 5 Scan Date : 2007-07-27 Scan Time : 21:17:52 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

treatment_protocol: Growth Condition : 25C YPD midlog

source_name: bayanus grown at 30 C

treatment_protocol: Growth Condition : 30C chemostat media + 0.24% glucose, midlog


title: Bleach Exposure (.25%, 5 min.)

description: Bleach Exposure (.25%, 5 min.)

scan_protocol: Scan Power : 100 PMT Volts : 518 Pixel Size : 5 Laser on time : 19876 Laser Power : 9.59 Scan Date : 2007-11-30 Scan Temperature : 29.96 Scan Time : 19:23:45 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.4
Datafile type : GenePix Results 3
Image Origin : 800, 3080
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

treatment_protocol:
Time : 5 minutes
Compound Based Treatment : 0.25% Bleach (NaOCl)
Growth Condition : 25C YPD


title: S. bayanus Tn insert 12 vs. Mixed Reference

description: S. bayanus Tn Insert 12 grown to log phase

scan_protocol: Pixel Size : 5 Scan Date : 2007-12-15 Scan Time : 20:19:19 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: S. bayanus Tn Insert 12 at log phase

value: Tn7 insert 12


title: S. bayanus Tn insert 18 vs. Mixed Reference

description: S. bayanus Tn insert 18

scan_protocol: Pixel Size : 5 Scan Date : 2008-01-03 Scan Time : 14:48:42 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

value: S. bayanus

source_name: S. bayanus Tn insert 18

value: Tn7 insert 18


title: Peroxide Treatment 30 min

description: Treated batch culture of S. bayanus with peroxide and took timepoints (Jul 9-12 2007, microarray class).

scan_protocol: Scan Power : 100 PMT Volts : 518 Pixel Size : 5 Laser on time : 11317 Laser Power : 4.28 Scan Date : 2007-07-11 Scan Temperature : 29.68 Scan Time : 11:31:07 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.2
Datafile type : GenePix Results 3
Image Origin : 640, 3200
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

source_name: Mixed reference

source_name: Peroxide 30 min

treatment_protocol:
Time : 30 minutes
Compound Based Treatment : 0.15 mM H2O2
Growth Condition : 25C YPD

value: prototroph


title: S. bayanus Tn insert 20 vs. Mixed Reference

description: S. bayanus Tn insert 20

scan_protocol: Pixel Size : 5 Scan Date : 2008-01-03 Scan Time : 15:40:08 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: S. bayanus Tn insert 20

value: Tn7 insert 20

value: MATa


title: Peroxide Treatment 20 min

scan_protocol: Pixel Size : 5 Scan Date : 2007-07-11 Scan Time : 11:25:44 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: Peroxide 20 min

treatment_protocol:
Compound Based Treatment : 0.15 mM H2O2
Growth Condition : 25C YPD
Time : 20 minutes

value: ACY12 prototroph MATalpha

value: mixed


title: S. bayanus Tn insert 16 vs. Mixed Reference

description: S. bayanus Tn insert 16

scan_protocol: Pixel Size : 5 Scan Date : 2008-01-03 Scan Time : 15:48:21 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: S. bayanus Tn insert 16

value: Tn7 insert 16


title: Peroxide Treatment 15 min

scan_protocol: Pixel Size : 5 Scan Date : 2007-07-11 Scan Time : 11:08:20 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: Peroxide 15 min

treatment_protocol:
Compound Based Treatment : 0.15 mM H2O2
Growth Condition : 25C YPD
Time : 15 minutes


title: Peroxide Challenge 30

description: 3O timepoint for S. bayanus peroxide challenge experiment Feline Cocktail

scan_protocol: Pixel Size : 5 Scan Date : 2007-07-11 Scan Time : 12:01:31 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: combined reference (class pool)

source_name: peroxide challenge 30 minutes

value: MATalpha


title: 5minH2O2

description: mRNA extracted from S. bayanus at 5mins after hydrogen peroxide shock

scan_protocol: Scan Power : 100 PMT Volts : 404 Pixel Size : 5 Laser on time : 41412 Laser Power : 2.27 Scan Date : 2007-07-11 Scan Temperature : 23.88 Scan Time : 11:10:26 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4200A 01  [111967] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.0.1.25
Datafile type : GenePix Results 3
Image Origin : 960, 2280
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

source_name: Mixed Reference

source_name: H2O2 5min

treatment_protocol:
Time : 5 minutes
Compound Based Treatment : 0.15 mM H2O2
Growth Condition : 25C YPD


title: S. bayanus Tn insert 26 vs. Mixed Reference

description: S. bayanus Tn insert 26

scan_protocol: Pixel Size : 5 Scan Date : 2008-01-04 Scan Time : 16:28:05 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: Mixed S. bayanus reference

source_name: S. bayanus Tn insert 26

value: Tn7 insert 26


title: S. bayanus Tn Insert 6 vs. Mixed Reference

description: S. bayanus Tn Insert 6 grown to log phase

scan_protocol: Pixel Size : 5 Scan Date : 2007-12-15 Scan Time : 20:02:32 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: S. bayanus Tn Insert 6 at log phase

value: Tn7 insert 6


title: 0minH2O2

description: mRNA extracted from S. bayanus right before hydrogen peroxide shock

scan_protocol: Pixel Size : 5 Scan Date : 2007-07-11 Scan Time : 10:58:47 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: H2O2 0min

treatment_protocol:
Time : 0 minutes
Compound Based Treatment : 0.15 mM H2O2
Growth Condition : 25C YPD


title: S. bayanus Tn insert 33 vs. Mixed Reference

description: S. bayanus Tn insert 33

scan_protocol: Pixel Size : 5 Scan Date : 2008-01-04 Scan Time : 17:14:18 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

treatment_protocol: Growth Condition : mixed

source_name: S. bayanus Tn insert 33

value: Tn7 insert 33


title: S. bayanus Tn insert 36 vs. Mixed Reference

description: S. bayanus Tn insert 36

scan_protocol: Pixel Size : 5 Scan Date : 2008-01-04 Scan Time : 16:36:18 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: S. bayanus Tn insert 36

value: Tn7 insert 36


title: Peroxide Challenge 20

description: 2O timepoint for S. bayanus peroxide challenge experiment Feline Cocktail

scan_protocol: Pixel Size : 5 Scan Date : 2007-07-11 Scan Time : 12:10:25 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: peroxide challenge 20 minutes

treatment_protocol:
Time : 20 minutes
Compound Based Treatment : 0.15 mM H2O2
Growth Condition : 25C YPD


title: S. bayanus Tn insert 24 vs. Mixed Reference

description: S. bayanus Tn insert 24

scan_protocol: Pixel Size : 5 Scan Date : 2008-01-04 Scan Time : 15:51:16 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: S. bayanus Tn insert 24

value: Tn7 insert 24


title: S. bayanus Tn insert 15 vs. Mixed Reference

description: S. bayanus Tn insert 15

scan_protocol: Pixel Size : 5 Scan Date : 2008-01-03 Scan Time : 15:31:44 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: S. bayanus Tn insert 15

value: Tn7 insert 15


title: Hydrogen Peroxide Exposure (10 min.)

description: Hydrogen Peroxide Exposure (10 min.)

scan_protocol: Scan Power : 100 PMT Volts : 493 Pixel Size : 5 Laser on time : 19432 Laser Power : 8.88 Scan Date : 2007-11-29 Scan Temperature : 27.67 Scan Time : 13:06:03 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.4
Datafile type : GenePix Results 3
Image Origin : 440, 2880
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

source_name: Exposed

treatment_protocol:
Time : 10 minutes
Compound Based Treatment : 0.3 mM H2O2
Growth Condition : 25C YPD


title: S. bayanus Tn insert 27 vs. Mixed Reference

description: S. bayanus Tn insert 27

scan_protocol: Pixel Size : 5 Scan Date : 2008-01-04 Scan Time : 16:19:11 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: S. bayanus Tn insert 27

value: Tn7 insert 27


title: Peroxide Challenge 15

description: 15 timepoint for S. bayanus peroxide challenge experiment Feline Cocktail

scan_protocol: Pixel Size : 5 Scan Date : 2007-07-11 Scan Time : 11:44:06 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: peroxide challenge 15 minutes

treatment_protocol:
Time : 15 minutes
Compound Based Treatment : 0.15 mM H2O2
Growth Condition : 25C YPD


title: S. bayanus Tn insert 25 vs. Mixed Reference

description: S. bayanus Tn insert 25

scan_protocol: Pixel Size : 5 Scan Date : 2008-01-04 Scan Time : 16:09:55 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: S. bayanus Tn insert 25

value: Tn7 insert 25


title: 10minH2O2

description: mRNA extracted from S. bayanus at 10mins after hydrogen peroxide shock

scan_protocol: Pixel Size : 5 Scan Date : 2007-07-11 Scan Time : 11:17:02 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

value: S. bayanus

source_name: H2O2 10min

treatment_protocol:
Time : 10 minutes
Compound Based Treatment : 0.15 mM H2O2
Growth Condition : 25C YPD


title: Peroxide Challenge 0

description: O timepoint for S. bayanus peroxide challenge experiment Feline Cocktail

scan_protocol: Pixel Size : 5 Scan Date : 2007-07-11 Scan Time : 10:49:55 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: combined reference (class pool)

source_name: peroxide challenge 0 minutes


title: S. bayanus Tn insert 32 vs. Mixed Reference

description: S. bayanus Tn insert 32

scan_protocol: Pixel Size : 5 Scan Date : 2008-01-04 Scan Time : 16:47:10 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: S. bayanus Tn insert 32

value: Tn7 insert 32


title: Hydrogen Peroxide Exposure (60 min.)

description: Hydrogen Peroxide Exposure (60 min.)

scan_protocol: Scan Power : 100 PMT Volts : 477 Pixel Size : 5 Laser on time : 19495 Laser Power : 9.29 Scan Date : 2007-11-29 Scan Temperature : 29.23 Scan Time : 14:11:09 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.4
Datafile type : GenePix Results 3
Image Origin : 2080, 3200
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

source_name: Mixed Reference

treatment_protocol:
Time : 60 minutes
Compound Based Treatment : 0.3 mM H2O2
Growth Condition : 25C YPD

value: prototroph


title: Hydrogen Peroxide Exposure (30 min.)

description: Hydrogen Peroxide Exposure (30 min.)

scan_protocol: Scan Power : 100 PMT Volts : 467 Pixel Size : 5 Laser on time : 19540 Laser Power : 9.33 Scan Date : 2007-11-29 Scan Temperature : 29.65 Scan Time : 14:56:09 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.4
Datafile type : GenePix Results 3
Image Origin : 1600, 3400
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

treatment_protocol:
Time : 30 minutes
Compound Based Treatment : 0.3 mM H2O2
Growth Condition : 25C YPD


title: S. bayanus Tn insert 28 vs. Mixed Reference

description: S. bayanus Tn insert 28

scan_protocol: Pixel Size : 5 Scan Date : 2008-01-04 Scan Time : 17:05:13 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: Mixed S. bayanus reference

source_name: S. bayanus Tn insert 28

value: Tn7 insert 28


title: Hydrogen Peroxide Exposure (20 min.)

description: Hydrogen Peroxide Exposure (20 min.)

scan_protocol: Scan Power : 100 PMT Volts : 484 Pixel Size : 5 Laser on time : 19472 Laser Power : 9.12 Scan Date : 2007-11-29 Scan Temperature : 28.86 Scan Time : 13:47:02 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.4
Datafile type : GenePix Results 3
Image Origin : 880, 2760
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

treatment_protocol:
Time : 20 minutes
Compound Based Treatment : 0.3 mM H2O2
Growth Condition : 25C YPD


title: LovastatinT0

description: S. bayanus. Drug: lovastatin. Time=0.

scan_protocol: Pixel Size : 5 Scan Date : 2007-09-27 Scan Time : 15:02:12 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: mixed reference S.  cerevisiae

source_name: S. bayanus

treatment_protocol:
Time : 0 minutes
Compound Based Treatment : 43 ug/mL lovastatin
Growth Condition : 25C YPD


title: Hydrogen Peroxide Exposure (0 min.)

description: Hydrogen Peroxide Exposure (0 min.)

scan_protocol: Scan Power : 100 PMT Volts : 507 Pixel Size : 5 Laser on time : 19137 Laser Power : 8.88 Scan Date : 2007-11-28 Scan Temperature : 28.65 Scan Time : 14:55:42 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.4
Datafile type : GenePix Results 3
Image Origin : 880, 3440
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

treatment_protocol:
Time : 0 minutes
Compound Based Treatment : 0.3 mM H2O2
Growth Condition : 25C YPD


title: oxidative stress time point

description: edit 3/4/2009: changed to swap

scan_protocol: Pixel Size : 5 Scan Date : 2007-07-11 Scan Time : 11:34:26 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: 5 minute time point

treatment_protocol:
Compound Based Treatment : 0.15 mM H2O2
Growth Condition : 25C YPD
Time : 5 minutes

value: ACY12, prototroph, MATalpha

source_name: reference (class mix)


title: Zinc Exposure (60 min.)

description: Zinc Exposure (60 min.)

scan_protocol: Scan Power : 100 PMT Volts : 545 Pixel Size : 5 Laser on time : 19663 Laser Power : 9.44 Scan Date : 2007-11-29 Scan Temperature : 30.57 Scan Time : 17:03:12 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.4
Datafile type : GenePix Results 3
Image Origin : 1040, 4760
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

treatment_protocol:
Time : 60 minutes
Compound Based Treatment : 1.5 mM ZnCl2
Growth Condition : 25C YPD


title: Zinc Exposure (10 min.)

description: Zinc Exposure (10 min.)

scan_protocol: Scan Power : 100 PMT Volts : 516 Pixel Size : 5 Laser on time : 19593 Laser Power : 9.37 Scan Date : 2007-11-29 Scan Temperature : 30.23 Scan Time : 15:52:40 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.4
Datafile type : GenePix Results 3
Image Origin : 1920, 3840
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

treatment_protocol:
Time : 10 minutes
Compound Based Treatment : 1.5 mM ZnCl2
Growth Condition : 25C YPD


title: S. bayanus Tn Insert 2 vs. Mixed Reference

description: S. bayanus Tn Insert 2 grown to log phase,

scan_protocol: Pixel Size : 5 Scan Date : 2007-12-15 Scan Time : 18:44:26 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 5.1.0.10
Datafile type : GenePix Results 3
Image Origin : 0, 0
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

source_name: S. bayanus Tn Insert 2 at log phase

treatment_protocol: Growth Condition : 25C YPD midlog

value: Tn7 insert 2

value: MATa


title: Copper Exposed (60 min.)

description: Copper Exposed (60 min.)

scan_protocol: Scan Power : 100 PMT Volts : 517 Pixel Size : 5 Laser on time : 20465 Laser Power : 10.00 Scan Date : 2007-12-01 Scan Temperature : 30.97 Scan Time : 18:12:03 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.4
Datafile type : GenePix Results 3
Image Origin : 920, 3960
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

value: mixed

source_name: Copper Exposed

treatment_protocol:
Time : 60 minutes
Compound Based Treatment : 2 mM CuSO4
Growth Condition : 25C YPD


title: S. bayanus Tn insert 21 vs. Mixed Reference

description: S. bayanus Tn insert 21

scan_protocol: Pixel Size : 5 Scan Date : 2008-01-03 Scan Time : 15:06:13 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: S. bayanus Tn insert 21

value: Tn7 insert 21


title: S. bayanus Tn insert 22 vs. Mixed Reference

description: S. bayanus Tn insert 22

scan_protocol: Pixel Size : 5 Scan Date : 2008-01-03 Scan Time : 14:32:02 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: S. bayanus Tn insert 22

value: Tn7 insert 22


title: Zinc Exposure (0 min.)

description: Zinc Exposure (0 min.)

scan_protocol: Scan Power : 100 PMT Volts : 523 Pixel Size : 5 Laser on time : 19518 Laser Power : 9.26 Scan Date : 2007-11-29 Scan Temperature : 29.44 Scan Time : 14:33:13 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.4
Datafile type : GenePix Results 3
Image Origin : 1280, 4160
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

source_name: Exposed

treatment_protocol:
Time : 0 minutes
Compound Based Treatment : 1.5 mM ZnCl2
Growth Condition : 25C YPD

value: MATalpha


title: S. bayanus Tn Insert 8 vs. Mixed Reference

description: S. bayanus Tn Insert 8 grown to log phase,

scan_protocol: Pixel Size : 5 Scan Date : 2007-12-15 Scan Time : 20:35:48 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: S. bayanus Tn Insert 8 at log phase

value: Tn7 insert 8


title: S. bayanus Tn Insert 7 vs. Mixed Reference

description: S. bayanus Tn Insert 7 grown to log phase,

scan_protocol: Pixel Size : 5 Scan Date : 2007-12-15 Scan Time : 19:18:52 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: S. bayanus Tn Insert 7 at log phase

value: Tn7 insert 7


title: Copper Exposed (20 min.)

description: Copper Exposed (20 min.)

scan_protocol: Scan Power : 100 PMT Volts : 512 Pixel Size : 5 Laser on time : 20552 Laser Power : 10.00 Scan Date : 2007-12-01 Scan Temperature : 31.09 Scan Time : 19:39:34 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.4
Datafile type : GenePix Results 3
Image Origin : 840, 3040
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

treatment_protocol:
Time : 20 minutes
Compound Based Treatment : 2 mM CuSO4
Growth Condition : 25C YPD


title: Zinc Exposure (20 min.)

description: Zinc Exposure (20 min.)

scan_protocol: Scan Power : 100 PMT Volts : 520 Pixel Size : 5 Laser on time : 19618 Laser Power : 9.41 Scan Date : 2007-11-29 Scan Temperature : 30.32 Scan Time : 16:18:28 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.4
Datafile type : GenePix Results 3
Image Origin : 1280, 3760
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

treatment_protocol: Growth Condition : mixed

treatment_protocol:
Time : 20 minutes
Compound Based Treatment : 1.5 mM ZnCl2
Growth Condition : 25C YPD


title: Zinc Exposure (30 min.)

description: Zinc Exposure (30 min.)

scan_protocol: Scan Power : 100 PMT Volts : 525 Pixel Size : 5 Laser on time : 19643 Laser Power : 9.42 Scan Date : 2007-11-29 Scan Temperature : 30.48 Scan Time : 16:43:08 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.4
Datafile type : GenePix Results 3
Image Origin : 800, 4320
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

treatment_protocol:
Time : 30 minutes
Compound Based Treatment : 1.5 mM ZnCl2
Growth Condition : 25C YPD


title: S. bayanus Tn insert 19 vs. Mixed Reference

description: S. bayanus Tn insert 19

scan_protocol: Pixel Size : 5 Scan Date : 2008-01-03 Scan Time : 15:22:29 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: S. bayanus Tn insert 19

value: Tn7 insert 19


title: S. bayanus Tn insert 14 vs. Mixed Reference

description: S. bayanus Tn insert 14

scan_protocol: Pixel Size : 5 Scan Date : 2008-01-03 Scan Time : 14:57:54 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: S. bayanus Tn insert 14

value: Tn7 insert 14


title: Zinc Exposure (5 min.)

description: Zinc Exposure (5 min.)

scan_protocol: Scan Power : 100 PMT Volts : 491 Pixel Size : 5 Laser on time : 19569 Laser Power : 9.33 Scan Date : 2007-11-29 Scan Temperature : 30.02 Scan Time : 15:24:31 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.4
Datafile type : GenePix Results 3
Image Origin : 1440, 3760
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

treatment_protocol:
Time : 5 minutes
Compound Based Treatment : 1.5 mM ZnCl2
Growth Condition : 25C YPD


title: Copper Exposed (10 min.)

description: Copper Exposed (10 min.)

scan_protocol: Scan Power : 100 PMT Volts : 557 Pixel Size : 5 Laser on time : 20288 Laser Power : 9.92 Scan Date : 2007-12-01 Scan Temperature : 30.63 Scan Time : 15:13:40 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.4
Datafile type : GenePix Results 3
Image Origin : 1400, 4600
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

source_name: Cadmium Exposed

treatment_protocol:
Time : 10 minutes
Compound Based Treatment : 2 mM CuSO4
Growth Condition : 25C YPD


title: Copper Exposed (5 min.)

description: Copper Exposed (5 min.)

scan_protocol: Scan Power : 100 PMT Volts : 503 Pixel Size : 5 Laser on time : 20600 Laser Power : 10.00 Scan Date : 2007-12-01 Scan Temperature : 31.21 Scan Time : 20:27:04 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.4
Datafile type : GenePix Results 3
Image Origin : 920, 3200
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

source_name: Mixed Reference

treatment_protocol:
Time : 5 minutes
Compound Based Treatment : 2 mM CuSO4
Growth Condition : 25C YPD


title: Copper Exposed (30 min.)

description: Copper Exposed (30 min.)

scan_protocol: Scan Power : 100 PMT Volts : 517 Pixel Size : 5 Laser on time : 20529 Laser Power : 10.00 Scan Date : 2007-12-01 Scan Temperature : 31.06 Scan Time : 19:17:01 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

treatment_protocol:
Time : 30 minutes
Compound Based Treatment : 2 mM CuSO4
Growth Condition : 25C YPD


title: 8D A7 vs. Mixed Reference

description: Segregant 8D A7 Kan+/lys, a

scan_protocol: Pixel Size : 5 Scan Date : 2007-12-17 Scan Time : 18:11:17 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: Mixed S. bayanus reference

source_name: 8D A7

value: 8D A7

value: Kan+/lys


title: 8D A6 vs. Mixed Reference

description: Segregant 8D A6 Kan-/LYS

scan_protocol: Pixel Size : 5 Scan Date : 2007-12-17 Scan Time : 17:14:02 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

value: S. bayanus

source_name: 8D A6

value: 8D A6

value: Kan-/LYS

value: MATa/alpha


title: Cadmium Exposed (20 min.)

description: Cadmium Exposed (20 min.)

scan_protocol: Scan Power : 100 PMT Volts : 463 Pixel Size : 5 Laser on time : 20420 Laser Power : 10.00 Scan Date : 2007-12-01 Scan Temperature : 30.72 Scan Time : 17:27:27 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.4
Datafile type : GenePix Results 3
Image Origin : 1720, 4280
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

treatment_protocol:
Time : 20 minutes
Compound Based Treatment : 5 uM CdCl2
Growth Condition : 25C YPD

value: prototroph


title: Hydrogen Peroxide Exposure (5 min.)

description: Hydrogen Peroxide Exposure (5 min.)

scan_protocol: Scan Power : 100 PMT Volts : 482 Pixel Size : 5 Laser on time : 19179 Laser Power : 9.01 Scan Date : 2007-11-28 Scan Temperature : 29.20 Scan Time : 15:37:59 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.4
Datafile type : GenePix Results 3
Image Origin : 480, 3120
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

treatment_protocol:
Time : 5 minutes
Compound Based Treatment : 0.3 mM H2O2
Growth Condition : 25C YPD


title: Copper Exposed (0 min.)

description: Copper Exposed (0 min.)

scan_protocol: Scan Power : 100 PMT Volts : 541 Pixel Size : 5 Laser on time : 20577 Laser Power : 10.00 Scan Date : 2007-12-01 Scan Temperature : 31.12 Scan Time : 20:04:35 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.4
Datafile type : GenePix Results 3
Image Origin : 1320, 2720
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

treatment_protocol:
Time : 0 minutes
Compound Based Treatment : 2 mM CuSO4
Growth Condition : 25C YPD


title: Cadmium Exposed (30 min.)

description: Cadmium Exposed (30 min.)

scan_protocol: Scan Power : 100 PMT Volts : 475 Pixel Size : 5 Laser on time : 20442 Laser Power : 10.00 Scan Date : 2007-12-01 Scan Temperature : 30.81 Scan Time : 17:49:53 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.4
Datafile type : GenePix Results 3
Image Origin : 1160, 3880
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

treatment_protocol:
Time : 30 minutes
Compound Based Treatment : 5 uM CdCl2
Growth Condition : 25C YPD


title: Cadmium Exposed (5 min.)

description: Cadmium Exposed (5 min.)

scan_protocol: Scan Power : 100 PMT Volts : 485 Pixel Size : 5 Laser on time : 20251 Laser Power : 9.85 Scan Date : 2007-12-01 Scan Temperature : 30.20 Scan Time : 14:36:34 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.4
Datafile type : GenePix Results 3
Image Origin : 1480, 4120
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

treatment_protocol:
Time : 5 minutes
Compound Based Treatment : 5 uM CdCl2
Growth Condition : 25C YPD


title: Sulfite Exposure (30 min.)

description: Sulfite Exposure (30 min.)

scan_protocol: Scan Power : 100 PMT Volts : 492 Pixel Size : 5 Laser on time : 19014 Laser Power : 9.02 Scan Date : 2007-11-23 Scan Temperature : 29.93 Scan Time : 00:30:08 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.4
Datafile type : GenePix Results 3
Image Origin : 800, 3120
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

source_name: Sulfite Exposed

treatment_protocol:
Time : 30 minutes
Compound Based Treatment : 10 mM K2S2O5
Growth Condition : 25C YPD


title: Sulfite Exposure (60 min.)

description: Sulfite Exposure (60 min.)

scan_protocol: Scan Power : 100 PMT Volts : 470 Pixel Size : 5 Laser on time : 18353 Laser Power : 8.40 Scan Date : 2007-11-21 Scan Temperature : 29.32 Scan Time : 12:30:34 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.4
Datafile type : GenePix Results 3
Image Origin : 560, 2680
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

treatment_protocol:
Time : 60 minutes
Compound Based Treatment : 10 mM K2S2O5
Growth Condition : 25C YPD


title: 6B A9 vs. Mixed Reference

description: Segregant 6B A9 Kan+/lys, alpha

scan_protocol: Pixel Size : 5 Scan Date : 2007-12-17 Scan Time : 16:40:10 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: 6B A9

treatment_protocol: Growth Condition : 25C YPD midlog

value: 6B A9


title: galactose Acy732 65 min

description: galactose Acy732 65 min

scan_protocol: Pixel Size : 5 Scan Date : 2008-12-19 Scan Time : 11:24:37 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.4
Datafile type : GenePix Results 3
Image Origin : 0, 0
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

source_name: sb common reference

source_name: galactose Acy732 65 min

treatment_protocol:
Time : 65 minutes
Growth Condition : 25C YNB + 2% galactose

value: prototroph 670.20del:KanMX


title: galactose Acy732 45 min

description: galactose Acy732 45 min

scan_protocol: Pixel Size : 5 Scan Date : 2008-12-19 Scan Time : 11:07:01 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

value: mixed

source_name: galactose Acy732 45 min

treatment_protocol:
Time : 45 minutes
Growth Condition : 25C YNB + 2% galactose


title: Sulfite Exposure (10 min.)

description: Sulfite Exposure (10 min.)

scan_protocol: Scan Power : 100 PMT Volts : 482 Pixel Size : 5 Laser on time : 18452 Laser Power : 8.56 Scan Date : 2007-11-21 Scan Temperature : 30.39 Scan Time : 14:10:00 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.4
Datafile type : GenePix Results 3
Image Origin : 160, 2120
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

treatment_protocol: Growth Condition : mixed

treatment_protocol:
Time : 10 minutes
Compound Based Treatment : 10 mM K2S2O5
Growth Condition : 25C YPD


title: Cadmium Exposed (0 min.)

description: Cadmium Exposed (0 min.)

scan_protocol: Scan Power : 100 PMT Volts : 485 Pixel Size : 5 Laser on time : 20231 Laser Power : 9.80 Scan Date : 2007-12-01 Scan Temperature : 29.87 Scan Time : 14:16:48 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.4
Datafile type : GenePix Results 3
Image Origin : 1000, 4120
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

treatment_protocol:
Time : 0 minutes
Compound Based Treatment : 5 uM CdCl2
Growth Condition : 25C YPD


title: Sulfite Exposure (20 min.)

description: Sulfite Exposure (20 min.)

scan_protocol: Scan Power : 100 PMT Volts : 464 Pixel Size : 5 Laser on time : 18410 Laser Power : 8.51 Scan Date : 2007-11-21 Scan Temperature : 30.14 Scan Time : 13:27:43 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.4
Datafile type : GenePix Results 3
Image Origin : 160, 1840
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

treatment_protocol:
Time : 20 minutes
Compound Based Treatment : 10 mM K2S2O5
Growth Condition : 25C YPD


title: galactose Acy732 145 min

description: galactose Acy732 145 min

scan_protocol: Pixel Size : 5 Scan Date : 2008-12-19 Scan Time : 10:40:30 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

source_name: galactose Acy732 145 min

treatment_protocol:
Time : 145 minutes
Growth Condition : 25C YNB + 2% galactose


title: 6B A8 vs. Mixed Reference

description: Segregant 6B A8 Kan-/LYS

scan_protocol: Pixel Size : 5 Scan Date : 2007-12-17 Scan Time : 16:49:15 Scanner Make : Agilent Technologies Scanner Scanner Model : G2505B Scanning software : ChipScan Scanning software version : A.7.0.1

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 5.1.0.10
Datafile type : GenePix Results 3
Image Origin : 0, 0
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

source_name: 6B A8

value: 6B A8


title: Ammonium Exposure (60 min.)

description: Ammonium Exposure (60 min.)

scan_protocol: Scan Power : 100 PMT Volts : 465 Pixel Size : 5 Laser on time : 20211 Laser Power : 9.61 Scan Date : 2007-12-01 Scan Temperature : 29.53 Scan Time : 13:56:36 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.4
Datafile type : GenePix Results 3
Image Origin : 1160, 4200
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

source_name: Ammonium Exposed

treatment_protocol:
Time : 60 minutes
Compound Based Treatment : 0.5 M NH4Cl
Growth Condition : 25C YPD


title: Sulfite Exposure (5 min.)

description: Sulfite Exposure (5 min.)

scan_protocol: Scan Power : 100 PMT Volts : 510 Pixel Size : 5 Laser on time : 18477 Laser Power : 8.57 Scan Date : 2007-11-21 Scan Temperature : 30.48 Scan Time : 14:34:18 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [116465] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : GenePix Pro
Extraction Software Version : 6.1.0.4
Datafile type : GenePix Results 3
Image Origin : 240, 2640
Normalization: Channel 2 values were scaled such that the average ratio for each array was set at 1. This scaling factor was calculated using only features from S. bayanus (some arrays also contain S. cerevisiae features that were not used for this project) with >65% of pixels >1 standard deviations above background in both channels.

treatment_protocol:
Time : 5 minutes
Compound Based Treatment : 10 mM K2S2O5
Growth Condition : 25C YPD