title: dH1F_iPS_1

status: Public on Jun 10 2010

description: Gene expression data obtained from fibroblasts iPS

data_processing: RMA (Bioconductor Affy and RMA Packages).

source_name: iPS

growth_protocol: For feeder-free culture of iPSCs, the plate was coated with 0.3mg/ml Matrigel (BD-Bioscience, San Jose, CA) and mTeSR (StemCell Technologies, Vancouver, Canada) was used according to the manufacturer’s instruction.

tag: cell type

value: iPS cells

tag: iPS derived from


title: PBMC(GH)_iPS1_2

type: RNA

city: Boston

first: Hu

last: Li

state: MA

address: 3 Blackfan Circle

country: USA

institute: Wyss Institute

description: Gene expression data obtained from PBMCs blood iPS

hyb_protocol: cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol

scan_protocol: Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation

label: biotin

molecule: total RNA

organism: Homo sapiens

label_protocol: Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA

extract_protocol: Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications

value: peripheral blood derived mononuclear cells (PBMCs)


title: Control Microglia Sample Y20 24hrs

status: Public on Dec 25 2004

city: Minneapolis

first: Robert

middle: Bryan

last: Rock

state: MN

institute: University of Minnesota

description:
Control human microglia sample Y20 sampled at 24hrs
Keywords = Microglia
Keywords = Interferon-gamma

source_name: Primary Human microglia


title: Control Microglia Sample O 24hrs

description:
Control human microglia sample O sampled at 24hrs
Keywords = Microglia
Keywords = Interferon-gamma


title: B6R 23

status: Public on Jan 08 2009

city: toronto

first: brian

last: knight

address: 25 orde st

country: Canada

institute: samuel lunenfeld research institute

description: no additional information

scan_protocol: standard protocols for Affymetrix GeneChip™3000 7G laser confocal slide scanner

data_processing: standard protocols for Affymetrix GeneChip® Mouse Gene 1.0 ST Array using Affymetrix Expression Console software

label: Biotin

organism: Mus musculus

source_name: hepatic tissue, C57BL/6J, prenatal undernutrition

extract_protocol: Total Hepatic RNA was extracted from the frozen tissue using RNeasy Mini Kits with on-column RNase-free DNase Kit digestion (Quigen)

treatment_protocol: pregnant mice were either treated as controls (C) and fed ad libitum for the duration of pregnancy or were treated as restricted (R) and subjected to undernutrition restriction diet. Whole mouse livers were extracted from 27 week old offspring from C or R treated mothers and flash frozen in liquid nitrogen.

tag: male 7month old offspring


title: B6R 21

description: no additional information

hyb_protocol: standard protocols for Affymetrix GeneChip® Mouse Gene 1.0 ST Array


title: dH1F_iPS_2

value: dH1F fibroblast


title: Control Microglia Sample W 24hrs

description:
Control human microglia sample W sampled at 24hrs
Keywords = Microglia
Keywords = Interferon-gamma


title: IFNG Microglia Sample Y20 24hrs

description:
Interferon-gamma treated (200u/ml) primary human microglial cells at 24 hrs post exposure
Keywords = Microglia
Keywords = Interferon-gamma

source_name: Primary human microglia


title: IFNG Microglia Sample W 6hrs

description:
Interferon-gamma treated (200u/ml) primary human microglial cells at 6 hrs post exposure
Keywords = Microglia
Keywords = Interferon-gamma


title: Control Microglia Sample B18 6hrs

description:
Control human microglia sample B18 sampled at 6hrs
Keywords = Microglia
Keywords = Interferon-gamma


title: B6R 7

description: no additional information

label_protocol: standard protocols for Affymetrix GeneChip® Mouse Gene 1.0 ST Array


title: PBMC(GH)_iPS1_1


title: Control Microglia Sample B18 24hrs

description:
Control human microglia sample B18 sampled at 24hrs
Keywords = Microglia
Keywords = Interferon-gamma


title: Control Microglia Sample Y20 1hr

description:
Control human microglia sample Y20 sampled at 1hr
Keywords = Microglia
Keywords = Interferon-gamma


title: IFNG Microglia Sample O 6hrs


title: IFNG Microglia Sample W 24hrs


title: Control Microglia Sample W 6hrs

description:
Control human microglia sample W sampled at 6hrs
Keywords = Microglia
Keywords = Interferon-gamma


title: IFNG Microglia Sample O 1hr

description:
Interferon-gamma treated (200u/ml) primary human microglial cells at 1 hr post exposure
Keywords = Microglia
Keywords = Interferon-gamma


title: IFNG Microglia Sample B18 24hrs


title: IFNG Microglia Sample Y20 6hrs


title: Control Microglia Sample O 6hrs

description:
Control human microglia sample O sampled at 6hrs
Keywords = Microglia
Keywords = Interferon-gamma


title: IFNG Microglia Sample W 1hr


title: Control Microglia Sample O 1hr

description:
Control human microglia sample O sampled at 1hr
Keywords = Microglia
Keywords = Interferon-gamma


title: IFNG Microglia Sample O 24hrs


title: Control Microglia Sample Y20 6hrs

description:
Control human microglia sample Y20 sampled at 6hrs
Keywords = Microglia
Keywords = Interferon-gamma


title: IFNG Microglia Sample Y20 1hr


title: Control Microglia Sample B18 1hr

description:
Control human microglia sample B18 sampled at 1hr
Keywords = Microglia
Keywords = Interferon-gamma


title: IFNG Microglia Sample B18 6hrs


title: Control Microglia Sample W 1hr

description:
Control human microglia sample W sampled at 1hr
Keywords = Microglia
Keywords = Interferon-gamma


title: IFNG Microglia Sample B18 1hr