title: Blood_0_424

description: Hybridized to Axiom®_Exome_319


title: Blood_0_400

status: Public on Jun 11 2014

type: genomic

city: Gyeonggi-do

first: Boyoung

last: Park

address: 323 Ilsan-ro, Ilsandong-gu, Goyang-si

country: South Korea

institute: National Cancer Centerm Korea

description: Hybridized to Axiom®_Exome_319

hyb_protocol: Following hybridization, the bound target is washed under stringent conditions to remove non-specific background to minimize background noise caused by random ligation events. Each polymorphic nucleotide is queried via a multi-color ligation event carried out on the array surface.

scan_protocol: After ligation, the arrays are stained and imaged on the GeneTitan MC Instrument (Affymetrix, Santa Clara, CA, USA)

data_processing: Using rawdata(cel files) as input, data analysis was conducted by Affymetrix power tools(APT) in linux server. APT-probeset-gneotype program, one of the APT applications, was used to gain genotype call as well as CHP files, which was run by birdseed-v2 algorithm as analysis option.

label: Biotin

molecule: genomic DNA

organism: Homo sapiens

source_name: peripheral blood leukocytes_control

label_protocol: About 200ng of genomic DNA is amplified and randomly fragmented into 25 to 125 base pair (bp) fragments. gDNA initial amplification reacted in 40㎕ reaction volume, containing 20㎕ volume of genomic DNA at a concentration of 10ng/㎕, 20㎕ of Denaturation Master Mix. The reaction of initial amplification carried out as follows: 10min at Room Temperature. After the initial amplification, the incubated products were amplicated with 130㎕ of Axiom 2.0 Neutral Soln, 225㎕ Axiom 2.0 Amp Soln and 5㎕ Axiom 2.0 Amp Enzyme. The amplification reactions were carried out as follows: 23hour ± 1hour at 37℃. The amplification products were performed in optimized reaction to amplify fragments between 200-1,100 base pairs. A fragmentation step then reduced the amplified products to segments of approximately 25-50 bp, which were then end-labeled using biotinylated nucleotides.

growth_protocol: Peripheral blood drawn from venous was stored in an icebox after obtaining to keep cool and was centrifused within one hour. After discarding supernatant, the blood was isolated as serum, plasma, RBC, whole blood, and buffy coat and stored in cryotube.

extract_protocol: After the blood samples are thawed, standard citrate buffer is added, mixed, and the tubes are centrifuged and the top portion of the supernatant is discarded (twice). The pellet is resuspended in a solution of SDS detergent and proteinase K, and the mixture is incubated at 55deg C for one hour. The sample then is phenol extracted once with a phenol/chloroform/isoamyl alcohol solution, and after centrifugation the aqueous layer is removed to a fresh microcentrifuge tube. The DNA is ethanol precipitated, resuspended in buffer, and then ethanol precipitated a second time. Once the pellet is dried, buffer is added and the DNA is resuspended by incubation.

treatment_protocol: The isolated samples are stored in -80deg C deep freezer.

tag: group

value: control

tag: gender

value: female


title: Blood_0_406

description: Hybridized to Axiom®_Exome_319


title: Blood_0_404

description: Hybridized to Axiom®_Exome_319

value: male


title: Blood_0_405

description: Hybridized to Axiom®_Exome_319


title: Blood_0_402

description: Hybridized to Axiom®_Exome_319


title: Blood_0_397

description: Hybridized to Axiom®_Exome_319


title: Blood_0_395

description: Hybridized to Axiom®_Exome_319


title: Blood_0_425

description: Hybridized to Axiom®_Exome_319


title: Blood_0_399

description: Hybridized to Axiom®_Exome_319


title: Blood_0_409

description: Hybridized to Axiom®_Exome_319


title: Blood_0_392

description: Hybridized to Axiom®_Exome_319


title: Blood_0_393

description: Hybridized to Axiom®_Exome_319


title: Blood_0_412

description: Hybridized to Axiom®_Exome_319


title: Blood_0_401

description: Hybridized to Axiom®_Exome_319


title: Blood_0_396

description: Hybridized to Axiom®_Exome_319


title: Blood_0_394

description: Hybridized to Axiom®_Exome_319


title: Blood_0_391

description: Hybridized to Axiom®_Exome_319


title: Blood_0_386

description: Hybridized to Axiom®_Exome_319


title: Blood_0_390

description: Hybridized to Axiom®_Exome_319


title: Blood_0_387

description: Hybridized to Axiom®_Exome_319


title: Blood_0_384

description: Hybridized to Axiom®_Exome_319


title: Blood_0_385

description: Hybridized to Axiom®_Exome_319


title: Blood_0_388

description: Hybridized to Axiom®_Exome_319


title: Blood_0_389

description: Hybridized to Axiom®_Exome_319


title: Blood_0_398

description: Hybridized to Axiom®_Exome_319


title: Blood_0_383

description: Hybridized to Axiom®_Exome_319


title: Blood_0_378

description: Hybridized to Axiom®_Exome_319


title: Blood_0_380

description: Hybridized to Axiom®_Exome_319


title: Blood_0_382

description: Hybridized to Axiom®_Exome_319


title: Blood_0_381

description: Hybridized to Axiom®_Exome_319


title: Blood_0_377

description: Hybridized to Axiom®_Exome_319


title: Blood_0_375

description: Hybridized to Axiom®_Exome_319


title: Blood_0_373

description: Hybridized to Axiom®_Exome_319


title: Blood_0_371

description: Hybridized to Axiom®_Exome_319


title: Blood_0_368

description: Hybridized to Axiom®_Exome_319


title: Blood_0_370

description: Hybridized to Axiom®_Exome_319


title: Blood_0_362

description: Hybridized to Axiom®_Exome_319


title: Blood_0_407

description: Hybridized to Axiom®_Exome_319


title: Blood_0_367

description: Hybridized to Axiom®_Exome_319


title: Blood_0_363

description: Hybridized to Axiom®_Exome_319


title: Blood_0_364

description: Hybridized to Axiom®_Exome_319


title: Blood_0_365

description: Hybridized to Axiom®_Exome_319


title: Blood_0_359

description: Hybridized to Axiom®_Exome_319


title: Blood_0_372

description: Hybridized to Axiom®_Exome_319


title: Blood_0_361

description: Hybridized to Axiom®_Exome_319


title: Blood_0_379

description: Hybridized to Axiom®_Exome_319


title: Blood_0_360

description: Hybridized to Axiom®_Exome_319


title: Blood_0_358

description: Hybridized to Axiom®_Exome_319


title: Blood_0_357

description: Hybridized to Axiom®_Exome_319


title: Blood_0_366

description: Hybridized to Axiom®_Exome_319


title: Blood_0_354

description: Hybridized to Axiom®_Exome_319


title: Blood_0_355

description: Hybridized to Axiom®_Exome_319


title: Blood_0_374

description: Hybridized to Axiom®_Exome_319


title: Blood_0_356

description: Hybridized to Axiom®_Exome_319


title: Blood_0_352

description: Hybridized to Axiom®_Exome_319


title: Blood_0_350

description: Hybridized to Axiom®_Exome_319


title: Blood_0_353

description: Hybridized to Axiom®_Exome_319


title: Blood_0_345

description: Hybridized to Axiom®_Exome_319


title: Blood_0_351

description: Hybridized to Axiom®_Exome_319


title: Blood_0_347

description: Hybridized to Axiom®_Exome_319


title: Blood_0_342

description: Hybridized to Axiom®_Exome_319


title: Blood_0_348

description: Hybridized to Axiom®_Exome_319


title: Blood_0_349

description: Hybridized to Axiom®_Exome_319


title: Blood_0_344

description: Hybridized to Axiom®_Exome_319


title: Blood_0_346

description: Hybridized to Axiom®_Exome_319


title: Blood_0_343

description: Hybridized to Axiom®_Exome_319


title: Blood_0_336

description: Hybridized to Axiom®_Exome_319


title: Blood_0_369

description: Hybridized to Axiom®_Exome_319


title: Blood_0_334

description: Hybridized to Axiom®_Exome_319


title: Blood_0_338

description: Hybridized to Axiom®_Exome_319


title: Blood_0_341

description: Hybridized to Axiom®_Exome_319


title: Blood_0_376

description: Hybridized to Axiom®_Exome_319


title: Blood_0_332

description: Hybridized to Axiom®_Exome_319


title: Blood_0_340

description: Hybridized to Axiom®_Exome_319


title: Blood_0_330

description: Hybridized to Axiom®_Exome_319


title: Blood_0_337

description: Hybridized to Axiom®_Exome_319


title: Blood_0_329

description: Hybridized to Axiom®_Exome_319


title: Blood_0_335

description: Hybridized to Axiom®_Exome_319


title: Blood_0_339

description: Hybridized to Axiom®_Exome_319


title: Blood_0_333

description: Hybridized to Axiom®_Exome_319


title: Blood_0_328

description: Hybridized to Axiom®_Exome_319


title: Blood_0_331

description: Hybridized to Axiom®_Exome_319


title: Blood_0_323

description: Hybridized to Axiom®_Exome_319


title: Blood_0_321

description: Hybridized to Axiom®_Exome_319


title: Blood_0_324

description: Hybridized to Axiom®_Exome_319


title: Blood_0_327

description: Hybridized to Axiom®_Exome_319


title: Blood_0_318

description: Hybridized to Axiom®_Exome_319


title: Blood_0_320

description: Hybridized to Axiom®_Exome_319


title: Blood_0_319

description: Hybridized to Axiom®_Exome_319


title: Blood_0_316

description: Hybridized to Axiom®_Exome_319


title: Blood_0_317

description: Hybridized to Axiom®_Exome_319


title: Blood_0_326

description: Hybridized to Axiom®_Exome_319


title: Blood_0_322

description: Hybridized to Axiom®_Exome_319


title: Blood_0_315

description: Hybridized to Axiom®_Exome_319


title: Blood_0_325

description: Hybridized to Axiom®_Exome_319


title: Blood_0_312

description: Hybridized to Axiom®_Exome_319


title: Blood_0_311

description: Hybridized to Axiom®_Exome_319


title: Blood_0_308

description: Hybridized to Axiom®_Exome_319


title: Blood_0_313

description: Hybridized to Axiom®_Exome_319


title: Blood_0_309

description: Hybridized to Axiom®_Exome_319


title: Blood_0_303

description: Hybridized to Axiom®_Exome_319


title: Blood_0_302

description: Hybridized to Axiom®_Exome_319


title: Blood_0_301

description: Hybridized to Axiom®_Exome_319


title: Blood_0_314

description: Hybridized to Axiom®_Exome_319


title: Blood_0_300

description: Hybridized to Axiom®_Exome_319


title: Blood_0_305

description: Hybridized to Axiom®_Exome_319


title: Blood_0_304

description: Hybridized to Axiom®_Exome_319


title: Blood_0_297

description: Hybridized to Axiom®_Exome_319


title: Blood_0_296

description: Hybridized to Axiom®_Exome_319


title: Blood_0_291

description: Hybridized to Axiom®_Exome_319


title: Blood_0_307

description: Hybridized to Axiom®_Exome_319


title: Blood_0_306

description: Hybridized to Axiom®_Exome_319


title: Blood_0_290

description: Hybridized to Axiom®_Exome_319


title: Blood_0_285

description: Hybridized to Axiom®_Exome_319


title: Blood_0_287

description: Hybridized to Axiom®_Exome_319


title: Blood_0_283

description: Hybridized to Axiom®_Exome_319


title: Blood_0_289

description: Hybridized to Axiom®_Exome_319


title: Blood_0_288

description: Hybridized to Axiom®_Exome_319


title: Blood_0_295

description: Hybridized to Axiom®_Exome_319


title: Blood_0_310

description: Hybridized to Axiom®_Exome_319


title: Blood_0_293

description: Hybridized to Axiom®_Exome_319


title: Blood_0_281

description: Hybridized to Axiom®_Exome_319


title: Blood_0_298

description: Hybridized to Axiom®_Exome_319


title: Blood_0_278

description: Hybridized to Axiom®_Exome_319


title: Blood_0_279

description: Hybridized to Axiom®_Exome_319


title: Blood_0_272

description: Hybridized to Axiom®_Exome_319


title: Blood_0_280

description: Hybridized to Axiom®_Exome_319


title: Blood_0_282

description: Hybridized to Axiom®_Exome_319


title: Blood_0_271

description: Hybridized to Axiom®_Exome_319


title: Blood_0_277

description: Hybridized to Axiom®_Exome_319


title: Blood_0_292

description: Hybridized to Axiom®_Exome_319


title: Blood_0_276

description: Hybridized to Axiom®_Exome_319


title: Blood_0_299

description: Hybridized to Axiom®_Exome_319


title: Blood_0_294

description: Hybridized to Axiom®_Exome_319


title: Blood_0_273

description: Hybridized to Axiom®_Exome_319


title: Blood_0_268

description: Hybridized to Axiom®_Exome_319


title: Blood_0_286

description: Hybridized to Axiom®_Exome_319


title: Blood_0_270

description: Hybridized to Axiom®_Exome_319


title: Blood_0_269

description: Hybridized to Axiom®_Exome_319


title: Blood_0_275

description: Hybridized to Axiom®_Exome_319


title: Blood_0_267

description: Hybridized to Axiom®_Exome_319


title: Blood_0_261

description: Hybridized to Axiom®_Exome_319


title: Blood_0_266

description: Hybridized to Axiom®_Exome_319


title: Blood_0_263

description: Hybridized to Axiom®_Exome_319


title: Blood_0_258

description: Hybridized to Axiom®_Exome_319


title: Blood_0_264

description: Hybridized to Axiom®_Exome_319


title: Blood_0_259

description: Hybridized to Axiom®_Exome_319


title: Blood_0_255

description: Hybridized to Axiom®_Exome_319


title: Blood_0_260

description: Hybridized to Axiom®_Exome_319


title: Blood_0_257

description: Hybridized to Axiom®_Exome_319


title: Blood_0_265

description: Hybridized to Axiom®_Exome_319


title: Blood_0_254

description: Hybridized to Axiom®_Exome_319


title: Blood_0_284

description: Hybridized to Axiom®_Exome_319


title: Blood_0_252

description: Hybridized to Axiom®_Exome_319


title: Blood_0_256

description: Hybridized to Axiom®_Exome_319


title: Blood_0_253

description: Hybridized to Axiom®_Exome_319


title: Blood_0_245

description: Hybridized to Axiom®_Exome_319


title: Blood_0_251

description: Hybridized to Axiom®_Exome_319


title: Blood_0_247

description: Hybridized to Axiom®_Exome_319


title: Blood_0_250

description: Hybridized to Axiom®_Exome_319


title: Blood_0_246

description: Hybridized to Axiom®_Exome_319


title: Blood_0_244

description: Hybridized to Axiom®_Exome_319


title: Blood_0_243

description: Hybridized to Axiom®_Exome_319


title: Blood_0_249

description: Hybridized to Axiom®_Exome_319


title: Blood_0_242

description: Hybridized to Axiom®_Exome_319


title: Blood_0_274

description: Hybridized to Axiom®_Exome_319


title: Blood_0_240

description: Hybridized to Axiom®_Exome_319


title: Blood_0_262

description: Hybridized to Axiom®_Exome_319


title: Blood_0_234

description: Hybridized to Axiom®_Exome_319


title: Blood_0_232

description: Hybridized to Axiom®_Exome_319


title: Blood_0_233

description: Hybridized to Axiom®_Exome_319


title: Blood_0_241

description: Hybridized to Axiom®_Exome_319


title: Blood_0_237

description: Hybridized to Axiom®_Exome_319


title: Blood_0_235

description: Hybridized to Axiom®_Exome_319


title: Blood_0_238

description: Hybridized to Axiom®_Exome_319


title: Blood_0_248

description: Hybridized to Axiom®_Exome_319


title: Blood_0_239

description: Hybridized to Axiom®_Exome_319


title: Blood_0_231

description: Hybridized to Axiom®_Exome_319


title: Blood_0_226

description: Hybridized to Axiom®_Exome_319


title: Blood_0_227

description: Hybridized to Axiom®_Exome_319


title: Blood_0_225

description: Hybridized to Axiom®_Exome_319


title: Blood_0_224

description: Hybridized to Axiom®_Exome_319


title: Blood_0_218

description: Hybridized to Axiom®_Exome_319


title: Blood_0_217

description: Hybridized to Axiom®_Exome_319


title: Blood_0_222

description: Hybridized to Axiom®_Exome_319


title: Blood_0_223

description: Hybridized to Axiom®_Exome_319


title: Blood_0_236

description: Hybridized to Axiom®_Exome_319


title: Blood_0_220

description: Hybridized to Axiom®_Exome_319


title: Blood_0_230

description: Hybridized to Axiom®_Exome_319


title: Blood_0_214

description: Hybridized to Axiom®_Exome_319


title: Blood_0_215

description: Hybridized to Axiom®_Exome_319


title: Blood_0_219

description: Hybridized to Axiom®_Exome_319


title: Blood_0_211

description: Hybridized to Axiom®_Exome_319


title: Blood_0_216

description: Hybridized to Axiom®_Exome_319


title: Blood_0_228

description: Hybridized to Axiom®_Exome_319


title: Blood_0_212

description: Hybridized to Axiom®_Exome_319


title: Blood_0_213

description: Hybridized to Axiom®_Exome_319


title: Blood_0_221

description: Hybridized to Axiom®_Exome_319


title: Blood_0_210

description: Hybridized to Axiom®_Exome_319


title: Blood_0_229

description: Hybridized to Axiom®_Exome_319


title: Blood_0_205

description: Hybridized to Axiom®_Exome_319


title: Blood_0_202

description: Hybridized to Axiom®_Exome_319


title: Blood_0_206

description: Hybridized to Axiom®_Exome_319


title: Blood_0_200

description: Hybridized to Axiom®_Exome_319


title: Blood_0_208

description: Hybridized to Axiom®_Exome_319


title: Blood_0_203

description: Hybridized to Axiom®_Exome_319


title: Blood_0_204

description: Hybridized to Axiom®_Exome_319


title: Blood_0_207

description: Hybridized to Axiom®_Exome_319


title: Blood_0_209

description: Hybridized to Axiom®_Exome_319


title: Blood_0_199

description: Hybridized to Axiom®_Exome_319


title: Blood_0_201

description: Hybridized to Axiom®_Exome_319


title: Blood_0_196

description: Hybridized to Axiom®_Exome_319


title: Blood_0_198

description: Hybridized to Axiom®_Exome_319


title: Blood_0_193

description: Hybridized to Axiom®_Exome_319


title: Blood_0_99

description: Hybridized to Axiom®_Exome_319


title: Blood_0_197

description: Hybridized to Axiom®_Exome_319


title: Blood_0_194

description: Hybridized to Axiom®_Exome_319


title: Blood_0_190

description: Hybridized to Axiom®_Exome_319


title: Blood_0_195

description: Hybridized to Axiom®_Exome_319


title: Blood_0_97

description: Hybridized to Axiom®_Exome_319


title: Blood_0_192

description: Hybridized to Axiom®_Exome_319


title: Blood_0_180

description: Hybridized to Axiom®_Exome_319


title: Blood_0_98

description: Hybridized to Axiom®_Exome_319


title: Blood_0_179

description: Hybridized to Axiom®_Exome_319


title: Blood_0_187

description: Hybridized to Axiom®_Exome_319


title: Blood_0_191

description: Hybridized to Axiom®_Exome_319


title: Blood_0_188

description: Hybridized to Axiom®_Exome_319


title: Blood_0_186

description: Hybridized to Axiom®_Exome_319


title: Blood_0_178

description: Hybridized to Axiom®_Exome_319


title: Blood_0_174

description: Hybridized to Axiom®_Exome_319


title: Blood_0_189

description: Hybridized to Axiom®_Exome_319


title: Blood_0_177

description: Hybridized to Axiom®_Exome_319


title: Blood_0_176

description: Hybridized to Axiom®_Exome_319


title: Blood_0_172

description: Hybridized to Axiom®_Exome_319


title: Blood_0_168

description: Hybridized to Axiom®_Exome_319


title: Blood_0_175

description: Hybridized to Axiom®_Exome_319


title: Blood_0_163

description: Hybridized to Axiom®_Exome_319


title: Blood_0_173

description: Hybridized to Axiom®_Exome_319


title: Blood_0_164

description: Hybridized to Axiom®_Exome_319


title: Blood_0_160

description: Hybridized to Axiom®_Exome_319


title: Blood_0_161

description: Hybridized to Axiom®_Exome_319


title: Blood_0_162

description: Hybridized to Axiom®_Exome_319


title: Blood_0_156

description: Hybridized to Axiom®_Exome_319


title: Blood_0_155

description: Hybridized to Axiom®_Exome_319


title: Blood_0_159

description: Hybridized to Axiom®_Exome_319


title: Blood_0_165

description: Hybridized to Axiom®_Exome_319


title: Blood_0_150

description: Hybridized to Axiom®_Exome_319


title: Blood_0_154

description: Hybridized to Axiom®_Exome_319


title: Blood_0_149

description: Hybridized to Axiom®_Exome_319


title: Blood_0_151

description: Hybridized to Axiom®_Exome_319


title: Blood_0_152

description: Hybridized to Axiom®_Exome_319


title: Blood_0_142

description: Hybridized to Axiom®_Exome_319


title: Blood_0_141

description: Hybridized to Axiom®_Exome_319


title: Blood_0_146

description: Hybridized to Axiom®_Exome_319


title: Blood_0_147

description: Hybridized to Axiom®_Exome_319


title: Blood_0_140

description: Hybridized to Axiom®_Exome_319


title: Blood_0_144

description: Hybridized to Axiom®_Exome_319


title: Blood_0_167

description: Hybridized to Axiom®_Exome_319


title: Blood_0_153

description: Hybridized to Axiom®_Exome_319


title: Blood_0_143

description: Hybridized to Axiom®_Exome_319


title: Blood_0_138

description: Hybridized to Axiom®_Exome_319


title: Blood_0_139

description: Hybridized to Axiom®_Exome_319


title: Blood_0_148

description: Hybridized to Axiom®_Exome_319


title: Blood_0_136

description: Hybridized to Axiom®_Exome_319


title: Blood_0_133

description: Hybridized to Axiom®_Exome_319


title: Blood_0_134

description: Hybridized to Axiom®_Exome_319


title: Blood_0_132

description: Hybridized to Axiom®_Exome_319


title: Blood_0_135

description: Hybridized to Axiom®_Exome_319


title: Blood_0_166

description: Hybridized to Axiom®_Exome_319


title: Blood_0_131

description: Hybridized to Axiom®_Exome_319


title: Blood_0_130

description: Hybridized to Axiom®_Exome_319


title: Blood_0_129

description: Hybridized to Axiom®_Exome_319


title: Blood_0_124

description: Hybridized to Axiom®_Exome_319


title: Blood_0_126

description: Hybridized to Axiom®_Exome_319


title: Blood_0_125

description: Hybridized to Axiom®_Exome_319


title: Blood_0_127

description: Hybridized to Axiom®_Exome_319


title: Blood_0_123

description: Hybridized to Axiom®_Exome_319


title: Blood_0_121

description: Hybridized to Axiom®_Exome_319


title: Blood_1_1017

description: Hybridized to Axiom®_Exome_319

source_name: peripheral blood leukocytes_case

value: gastric cancer case


title: Blood_0_122

description: Hybridized to Axiom®_Exome_319


title: Blood_0_181

description: Hybridized to Axiom®_Exome_319


title: Blood_0_137

description: Hybridized to Axiom®_Exome_319


title: Blood_0_119

description: Hybridized to Axiom®_Exome_319


title: Blood_1_1020

description: Hybridized to Axiom®_Exome_319


title: Blood_1_1019

description: Hybridized to Axiom®_Exome_319


title: Blood_0_120

description: Hybridized to Axiom®_Exome_319


title: Blood_0_158

description: Hybridized to Axiom®_Exome_319


title: Blood_1_1016

description: Hybridized to Axiom®_Exome_319


title: Blood_0_128

description: Hybridized to Axiom®_Exome_319


title: Blood_1_1013

description: Hybridized to Axiom®_Exome_319


title: Blood_0_169

description: Hybridized to Axiom®_Exome_319


title: Blood_0_170

description: Hybridized to Axiom®_Exome_319


title: Blood_1_1021

description: Hybridized to Axiom®_Exome_319


title: Blood_1_1012

description: Hybridized to Axiom®_Exome_319


title: Blood_1_1011

description: Hybridized to Axiom®_Exome_319


title: Blood_1_1010

description: Hybridized to Axiom®_Exome_319


title: Blood_1_1007

description: Hybridized to Axiom®_Exome_319


title: Blood_1_1015

description: Hybridized to Axiom®_Exome_319


title: Blood_1_1008

description: Hybridized to Axiom®_Exome_319


title: Blood_1_1005

description: Hybridized to Axiom®_Exome_319


title: Blood_1_1006

description: Hybridized to Axiom®_Exome_319


title: Blood_1_1003

description: Hybridized to Axiom®_Exome_319


title: Blood_1_1001

description: Hybridized to Axiom®_Exome_319


title: Blood_1_1000

description: Hybridized to Axiom®_Exome_319


title: Blood_0_171

description: Hybridized to Axiom®_Exome_319


title: Blood_1_955

description: Hybridized to Axiom®_Exome_319


title: Blood_1_961

description: Hybridized to Axiom®_Exome_319


title: Blood_1_1014

description: Hybridized to Axiom®_Exome_319


title: Blood_1_982

description: Hybridized to Axiom®_Exome_319


title: Blood_1_959

description: Hybridized to Axiom®_Exome_319


title: Blood_1_948

description: Hybridized to Axiom®_Exome_319


title: Blood_1_950

description: Hybridized to Axiom®_Exome_319


title: Blood_1_947

description: Hybridized to Axiom®_Exome_319


title: Blood_1_949

description: Hybridized to Axiom®_Exome_319


title: Blood_1_952

description: Hybridized to Axiom®_Exome_319


title: Blood_1_957

description: Hybridized to Axiom®_Exome_319


title: Blood_1_958

description: Hybridized to Axiom®_Exome_319


title: Blood_1_946

description: Hybridized to Axiom®_Exome_319


title: Blood_1_945

description: Hybridized to Axiom®_Exome_319


title: Blood_1_941

description: Hybridized to Axiom®_Exome_319


title: Blood_1_954

description: Hybridized to Axiom®_Exome_319


title: Blood_1_940

description: Hybridized to Axiom®_Exome_319


title: Blood_1_943

description: Hybridized to Axiom®_Exome_319


title: Blood_1_1018

description: Hybridized to Axiom®_Exome_319


title: Blood_1_944

description: Hybridized to Axiom®_Exome_319


title: Blood_1_938

description: Hybridized to Axiom®_Exome_319


title: Blood_1_951

description: Hybridized to Axiom®_Exome_319


title: Blood_1_936

description: Hybridized to Axiom®_Exome_319


title: Blood_1_1009

description: Hybridized to Axiom®_Exome_319


title: Blood_1_931

description: Hybridized to Axiom®_Exome_319


title: Blood_1_928

description: Hybridized to Axiom®_Exome_319


title: Blood_1_929

description: Hybridized to Axiom®_Exome_319


title: Blood_1_1004

description: Hybridized to Axiom®_Exome_319


title: Blood_1_1002

description: Hybridized to Axiom®_Exome_319


title: Blood_1_927

description: Hybridized to Axiom®_Exome_319


title: Blood_1_926

description: Hybridized to Axiom®_Exome_319


title: Blood_1_934

description: Hybridized to Axiom®_Exome_319


title: Blood_1_930

description: Hybridized to Axiom®_Exome_319


title: Blood_1_922

description: Hybridized to Axiom®_Exome_319


title: Blood_1_932

description: Hybridized to Axiom®_Exome_319


title: Blood_1_916

description: Hybridized to Axiom®_Exome_319


title: Blood_1_918

description: Hybridized to Axiom®_Exome_319


title: Blood_1_921

description: Hybridized to Axiom®_Exome_319


title: Blood_1_923

description: Hybridized to Axiom®_Exome_319


title: Blood_1_913

description: Hybridized to Axiom®_Exome_319


title: Blood_1_915

description: Hybridized to Axiom®_Exome_319


title: Blood_1_912

description: Hybridized to Axiom®_Exome_319


title: Blood_1_919

description: Hybridized to Axiom®_Exome_319


title: Blood_1_925

description: Hybridized to Axiom®_Exome_319


title: Blood_1_911

description: Hybridized to Axiom®_Exome_319


title: Blood_1_914

description: Hybridized to Axiom®_Exome_319


title: Blood_1_909

description: Hybridized to Axiom®_Exome_319


title: Blood_1_910

description: Hybridized to Axiom®_Exome_319


title: Blood_1_907

description: Hybridized to Axiom®_Exome_319


title: Blood_1_939

description: Hybridized to Axiom®_Exome_319


title: Blood_1_920

description: Hybridized to Axiom®_Exome_319


title: Blood_1_902

description: Hybridized to Axiom®_Exome_319


title: Blood_1_906

description: Hybridized to Axiom®_Exome_319


title: Blood_1_897

description: Hybridized to Axiom®_Exome_319


title: Blood_1_903

description: Hybridized to Axiom®_Exome_319


title: Blood_1_899

description: Hybridized to Axiom®_Exome_319


title: Blood_1_900

description: Hybridized to Axiom®_Exome_319


title: Blood_1_901

description: Hybridized to Axiom®_Exome_319


title: Blood_1_896

description: Hybridized to Axiom®_Exome_319


title: Blood_1_898

description: Hybridized to Axiom®_Exome_319


title: Blood_1_908

description: Hybridized to Axiom®_Exome_319


title: Blood_1_891

description: Hybridized to Axiom®_Exome_319


title: Blood_1_889

description: Hybridized to Axiom®_Exome_319


title: Blood_1_893

description: Hybridized to Axiom®_Exome_319


title: Blood_1_895

description: Hybridized to Axiom®_Exome_319


title: Blood_1_883

description: Hybridized to Axiom®_Exome_319


title: Blood_1_894

description: Hybridized to Axiom®_Exome_319


title: Blood_1_885

description: Hybridized to Axiom®_Exome_319


title: Blood_1_890

description: Hybridized to Axiom®_Exome_319


title: Blood_1_886

description: Hybridized to Axiom®_Exome_319


title: Blood_1_878

description: Hybridized to Axiom®_Exome_319


title: Blood_1_879

description: Hybridized to Axiom®_Exome_319


title: Blood_1_882

description: Hybridized to Axiom®_Exome_319


title: Blood_1_876

description: Hybridized to Axiom®_Exome_319


title: Blood_1_867

description: Hybridized to Axiom®_Exome_319


title: Blood_1_868

description: Hybridized to Axiom®_Exome_319


title: Blood_1_888

description: Hybridized to Axiom®_Exome_319


title: Blood_1_874

description: Hybridized to Axiom®_Exome_319


title: Blood_1_881

description: Hybridized to Axiom®_Exome_319


title: Blood_1_880

description: Hybridized to Axiom®_Exome_319


title: Blood_1_905

description: Hybridized to Axiom®_Exome_319


title: Blood_1_892

description: Hybridized to Axiom®_Exome_319


title: Blood_1_873

description: Hybridized to Axiom®_Exome_319


title: Blood_1_872

description: Hybridized to Axiom®_Exome_319


title: Blood_1_864

description: Hybridized to Axiom®_Exome_319


title: Blood_1_866

description: Hybridized to Axiom®_Exome_319


title: Blood_1_863

description: Hybridized to Axiom®_Exome_319


title: Blood_1_860

description: Hybridized to Axiom®_Exome_319


title: Blood_1_884

description: Hybridized to Axiom®_Exome_319


title: Blood_1_877

description: Hybridized to Axiom®_Exome_319


title: Blood_1_870

description: Hybridized to Axiom®_Exome_319


title: Blood_1_858

description: Hybridized to Axiom®_Exome_319


title: Blood_1_859

description: Hybridized to Axiom®_Exome_319


title: Blood_1_853

description: Hybridized to Axiom®_Exome_319


title: Blood_1_857

description: Hybridized to Axiom®_Exome_319


title: Blood_1_854

description: Hybridized to Axiom®_Exome_319


title: Blood_1_856

description: Hybridized to Axiom®_Exome_319


title: Blood_1_851

description: Hybridized to Axiom®_Exome_319


title: Blood_1_855

description: Hybridized to Axiom®_Exome_319


title: Blood_1_852

description: Hybridized to Axiom®_Exome_319


title: Blood_1_845

description: Hybridized to Axiom®_Exome_319


title: Blood_1_862

description: Hybridized to Axiom®_Exome_319


title: Blood_1_850

description: Hybridized to Axiom®_Exome_319


title: Blood_1_849

description: Hybridized to Axiom®_Exome_319


title: Blood_1_861

description: Hybridized to Axiom®_Exome_319


title: Blood_1_846

description: Hybridized to Axiom®_Exome_319


title: Blood_1_840

description: Hybridized to Axiom®_Exome_319


title: Blood_1_841

description: Hybridized to Axiom®_Exome_319


title: Blood_1_843

description: Hybridized to Axiom®_Exome_319


title: Blood_1_839

description: Hybridized to Axiom®_Exome_319


title: Blood_1_838

description: Hybridized to Axiom®_Exome_319


title: Blood_1_848

description: Hybridized to Axiom®_Exome_319


title: Blood_1_842

description: Hybridized to Axiom®_Exome_319


title: Blood_1_844

description: Hybridized to Axiom®_Exome_319


title: Blood_1_847

description: Hybridized to Axiom®_Exome_319


title: Blood_1_833

description: Hybridized to Axiom®_Exome_319


title: Blood_1_837

description: Hybridized to Axiom®_Exome_319


title: Blood_1_829

description: Hybridized to Axiom®_Exome_319


title: Blood_1_834

description: Hybridized to Axiom®_Exome_319


title: Blood_1_832

description: Hybridized to Axiom®_Exome_319


title: Blood_1_831

description: Hybridized to Axiom®_Exome_319


title: Blood_1_835

description: Hybridized to Axiom®_Exome_319


title: Blood_1_828

description: Hybridized to Axiom®_Exome_319


title: Blood_0_117

description: Hybridized to Axiom®_Exome_319


title: Blood_1_836

description: Hybridized to Axiom®_Exome_319


title: Blood_1_826

description: Hybridized to Axiom®_Exome_319


title: Blood_1_830

description: Hybridized to Axiom®_Exome_319


title: Blood_0_114

description: Hybridized to Axiom®_Exome_319


title: Blood_0_116

description: Hybridized to Axiom®_Exome_319


title: Blood_0_118

description: Hybridized to Axiom®_Exome_319


title: Blood_1_824

description: Hybridized to Axiom®_Exome_319


title: Blood_0_112

description: Hybridized to Axiom®_Exome_319


title: Blood_0_115

description: Hybridized to Axiom®_Exome_319


title: Blood_1_825

description: Hybridized to Axiom®_Exome_319


title: Blood_0_109

description: Hybridized to Axiom®_Exome_319


title: Blood_1_818

description: Hybridized to Axiom®_Exome_319


title: Blood_0_110

description: Hybridized to Axiom®_Exome_319


title: Blood_1_823

description: Hybridized to Axiom®_Exome_319


title: Blood_1_817

description: Hybridized to Axiom®_Exome_319


title: Blood_1_827

description: Hybridized to Axiom®_Exome_319


title: Blood_0_111

description: Hybridized to Axiom®_Exome_319


title: Blood_1_821

description: Hybridized to Axiom®_Exome_319


title: Blood_1_820

description: Hybridized to Axiom®_Exome_319


title: Blood_1_814

description: Hybridized to Axiom®_Exome_319


title: Blood_0_108

description: Hybridized to Axiom®_Exome_319


title: Blood_1_812

description: Hybridized to Axiom®_Exome_319


title: Blood_0_103

description: Hybridized to Axiom®_Exome_319


title: Blood_1_815

description: Hybridized to Axiom®_Exome_319


title: Blood_0_105

description: Hybridized to Axiom®_Exome_319


title: Blood_1_819

description: Hybridized to Axiom®_Exome_319


title: Blood_0_104

description: Hybridized to Axiom®_Exome_319


title: Blood_0_107

description: Hybridized to Axiom®_Exome_319


title: Blood_0_106

description: Hybridized to Axiom®_Exome_319


title: Blood_1_816

description: Hybridized to Axiom®_Exome_319


title: Blood_1_810

description: Hybridized to Axiom®_Exome_319


title: Blood_1_813

description: Hybridized to Axiom®_Exome_319


title: Blood_0_102

description: Hybridized to Axiom®_Exome_319


title: Blood_0_113

description: Hybridized to Axiom®_Exome_319


title: Blood_0_96

description: Hybridized to Axiom®_Exome_319


title: Blood_1_811

description: Hybridized to Axiom®_Exome_319


title: Blood_1_809

description: Hybridized to Axiom®_Exome_319


title: Blood_0_9

description: Hybridized to Axiom®_Exome_319


title: Blood_0_101

description: Hybridized to Axiom®_Exome_319


title: Blood_1_807

description: Hybridized to Axiom®_Exome_319


title: Blood_0_100

description: Hybridized to Axiom®_Exome_319


title: Blood_0_94

description: Hybridized to Axiom®_Exome_319


title: Blood_1_808

description: Hybridized to Axiom®_Exome_319


title: Blood_0_93

description: Hybridized to Axiom®_Exome_319


title: Blood_0_95

description: Hybridized to Axiom®_Exome_319


title: Blood_0_91

description: Hybridized to Axiom®_Exome_319


title: Blood_1_805

description: Hybridized to Axiom®_Exome_319


title: Blood_1_806

description: Hybridized to Axiom®_Exome_319


title: Blood_1_802

description: Hybridized to Axiom®_Exome_319


title: Blood_1_804

description: Hybridized to Axiom®_Exome_319


title: Blood_0_92

description: Hybridized to Axiom®_Exome_319


title: Blood_1_800

description: Hybridized to Axiom®_Exome_319


title: Blood_0_90

description: Hybridized to Axiom®_Exome_319


title: Blood_1_803

description: Hybridized to Axiom®_Exome_319


title: Blood_1_801

description: Hybridized to Axiom®_Exome_319


title: Blood_0_89

description: Hybridized to Axiom®_Exome_319


title: Blood_1_799

description: Hybridized to Axiom®_Exome_319


title: Blood_0_86

description: Hybridized to Axiom®_Exome_319


title: Blood_0_85

description: Hybridized to Axiom®_Exome_319


title: Blood_0_88

description: Hybridized to Axiom®_Exome_319


title: Blood_1_796

description: Hybridized to Axiom®_Exome_319


title: Blood_0_83

description: Hybridized to Axiom®_Exome_319


title: Blood_1_798

description: Hybridized to Axiom®_Exome_319


title: Blood_0_8

description: Hybridized to Axiom®_Exome_319


title: Blood_1_795

description: Hybridized to Axiom®_Exome_319


title: Blood_0_84

description: Hybridized to Axiom®_Exome_319


title: Blood_1_822

description: Hybridized to Axiom®_Exome_319


title: Blood_1_797

description: Hybridized to Axiom®_Exome_319


title: Blood_0_87

description: Hybridized to Axiom®_Exome_319


title: Blood_1_793

description: Hybridized to Axiom®_Exome_319


title: Blood_1_791

description: Hybridized to Axiom®_Exome_319


title: Blood_1_794

description: Hybridized to Axiom®_Exome_319


title: Blood_1_792

description: Hybridized to Axiom®_Exome_319


title: Blood_0_82

description: Hybridized to Axiom®_Exome_319


title: Blood_0_81

description: Hybridized to Axiom®_Exome_319


title: Blood_0_80

description: Hybridized to Axiom®_Exome_319


title: Blood_1_789

description: Hybridized to Axiom®_Exome_319


title: Blood_1_787

description: Hybridized to Axiom®_Exome_319


title: Blood_1_790

description: Hybridized to Axiom®_Exome_319


title: Blood_1_786

description: Hybridized to Axiom®_Exome_319


title: Blood_0_78

description: Hybridized to Axiom®_Exome_319


title: Blood_0_77

description: Hybridized to Axiom®_Exome_319


title: Blood_0_76

description: Hybridized to Axiom®_Exome_319


title: Blood_0_75

description: Hybridized to Axiom®_Exome_319


title: Blood_1_788

description: Hybridized to Axiom®_Exome_319


title: Blood_0_74

description: Hybridized to Axiom®_Exome_319


title: Blood_0_79

description: Hybridized to Axiom®_Exome_319


title: Blood_0_72

description: Hybridized to Axiom®_Exome_319


title: Blood_1_785

description: Hybridized to Axiom®_Exome_319


title: Blood_1_782

description: Hybridized to Axiom®_Exome_319


title: Blood_1_783

description: Hybridized to Axiom®_Exome_319


title: Blood_0_71

description: Hybridized to Axiom®_Exome_319


title: Blood_0_6

description: Hybridized to Axiom®_Exome_319


title: Blood_1_779

description: Hybridized to Axiom®_Exome_319


title: Blood_1_780

description: Hybridized to Axiom®_Exome_319


title: Blood_0_70

description: Hybridized to Axiom®_Exome_319


title: Blood_0_73

description: Hybridized to Axiom®_Exome_319


title: Blood_0_60

description: Hybridized to Axiom®_Exome_319


title: Blood_0_69

description: Hybridized to Axiom®_Exome_319


title: Blood_0_67

description: Hybridized to Axiom®_Exome_319


title: Blood_0_68

description: Hybridized to Axiom®_Exome_319


title: Blood_0_65

description: Hybridized to Axiom®_Exome_319


title: Blood_0_66

description: Hybridized to Axiom®_Exome_319


title: Blood_0_5

description: Hybridized to Axiom®_Exome_319


title: Blood_1_777

description: Hybridized to Axiom®_Exome_319


title: Blood_0_62

description: Hybridized to Axiom®_Exome_319


title: Blood_0_63

description: Hybridized to Axiom®_Exome_319


title: Blood_0_61

description: Hybridized to Axiom®_Exome_319


title: Blood_1_778

description: Hybridized to Axiom®_Exome_319


title: Blood_0_57

description: Hybridized to Axiom®_Exome_319


title: Blood_0_59

description: Hybridized to Axiom®_Exome_319


title: Blood_0_54

description: Hybridized to Axiom®_Exome_319


title: Blood_1_781

description: Hybridized to Axiom®_Exome_319


title: Blood_0_56

description: Hybridized to Axiom®_Exome_319


title: Blood_0_64

description: Hybridized to Axiom®_Exome_319


title: Blood_0_52

description: Hybridized to Axiom®_Exome_319


title: Blood_0_58

description: Hybridized to Axiom®_Exome_319


title: Blood_0_53

description: Hybridized to Axiom®_Exome_319


title: Blood_0_50

description: Hybridized to Axiom®_Exome_319


title: Blood_0_51

description: Hybridized to Axiom®_Exome_319


title: Blood_0_43

description: Hybridized to Axiom®_Exome_319


title: Blood_0_45

description: Hybridized to Axiom®_Exome_319


title: Blood_0_4

description: Hybridized to Axiom®_Exome_319


title: Blood_0_49

description: Hybridized to Axiom®_Exome_319


title: Blood_0_48

description: Hybridized to Axiom®_Exome_319


title: Blood_1_784

description: Hybridized to Axiom®_Exome_319


title: Blood_0_41

description: Hybridized to Axiom®_Exome_319


title: Blood_0_47

description: Hybridized to Axiom®_Exome_319


title: Blood_0_46

description: Hybridized to Axiom®_Exome_319


title: Blood_0_44

description: Hybridized to Axiom®_Exome_319


title: Blood_0_40

description: Hybridized to Axiom®_Exome_319


title: Blood_0_3

description: Hybridized to Axiom®_Exome_319


title: Blood_0_39

description: Hybridized to Axiom®_Exome_319


title: Blood_0_42

description: Hybridized to Axiom®_Exome_319


title: Blood_0_34

description: Hybridized to Axiom®_Exome_319


title: Blood_0_33

description: Hybridized to Axiom®_Exome_319


title: Blood_0_32

description: Hybridized to Axiom®_Exome_319


title: Blood_0_35

description: Hybridized to Axiom®_Exome_319


title: Blood_0_36

description: Hybridized to Axiom®_Exome_319


title: Blood_0_28

description: Hybridized to Axiom®_Exome_319


title: Blood_0_2

description: Hybridized to Axiom®_Exome_319


title: Blood_0_24

description: Hybridized to Axiom®_Exome_319


title: Blood_0_38

description: Hybridized to Axiom®_Exome_319


title: Blood_0_29

description: Hybridized to Axiom®_Exome_319


title: Blood_0_27

description: Hybridized to Axiom®_Exome_319


title: Blood_0_20

description: Hybridized to Axiom®_Exome_319


title: Blood_0_22

description: Hybridized to Axiom®_Exome_319


title: Blood_0_26

description: Hybridized to Axiom®_Exome_319


title: Blood_0_1

description: Hybridized to Axiom®_Exome_319


title: Blood_0_23

description: Hybridized to Axiom®_Exome_319


title: Blood_0_31

description: Hybridized to Axiom®_Exome_319


title: Blood_0_13

description: Hybridized to Axiom®_Exome_319


title: Blood_0_37

description: Hybridized to Axiom®_Exome_319


title: Blood_0_10

description: Hybridized to Axiom®_Exome_319


title: Blood_0_19

description: Hybridized to Axiom®_Exome_319


title: Blood_0_55

description: Hybridized to Axiom®_Exome_319


title: Blood_0_30

description: Hybridized to Axiom®_Exome_319


title: Blood_0_17

description: Hybridized to Axiom®_Exome_319


title: Blood_0_18

description: Hybridized to Axiom®_Exome_319


title: Blood_0_15

description: Hybridized to Axiom®_Exome_319


title: Blood_0_21

description: Hybridized to Axiom®_Exome_319


title: Blood_1_997

description: Hybridized to Axiom®_Exome_319


title: Blood_1_996

description: Hybridized to Axiom®_Exome_319


title: Blood_1_998

description: Hybridized to Axiom®_Exome_319


title: Blood_0_11

description: Hybridized to Axiom®_Exome_319


title: Blood_1_993

description: Hybridized to Axiom®_Exome_319


title: Blood_0_14

description: Hybridized to Axiom®_Exome_319


title: Blood_1_999

description: Hybridized to Axiom®_Exome_319


title: Blood_1_992

description: Hybridized to Axiom®_Exome_319


title: Blood_1_991

description: Hybridized to Axiom®_Exome_319


title: Blood_1_994

description: Hybridized to Axiom®_Exome_319


title: Blood_1_989

description: Hybridized to Axiom®_Exome_319


title: Blood_1_990

description: Hybridized to Axiom®_Exome_319


title: Blood_1_987

description: Hybridized to Axiom®_Exome_319


title: Blood_1_995

description: Hybridized to Axiom®_Exome_319


title: Blood_1_973

description: Hybridized to Axiom®_Exome_319


title: Blood_1_980

description: Hybridized to Axiom®_Exome_319


title: Blood_1_974

description: Hybridized to Axiom®_Exome_319


title: Blood_1_981

description: Hybridized to Axiom®_Exome_319


title: Blood_1_975

description: Hybridized to Axiom®_Exome_319


title: Blood_1_985

description: Hybridized to Axiom®_Exome_319


title: Blood_1_988

description: Hybridized to Axiom®_Exome_319


title: Blood_1_968

description: Hybridized to Axiom®_Exome_319


title: Blood_1_979

description: Hybridized to Axiom®_Exome_319


title: Blood_1_971

description: Hybridized to Axiom®_Exome_319


title: Blood_1_983

description: Hybridized to Axiom®_Exome_319


title: Blood_1_977

description: Hybridized to Axiom®_Exome_319


title: Blood_1_984

description: Hybridized to Axiom®_Exome_319


title: Blood_1_986

description: Hybridized to Axiom®_Exome_319


title: Blood_1_967

description: Hybridized to Axiom®_Exome_319


title: Blood_1_978

description: Hybridized to Axiom®_Exome_319


title: Blood_1_970

description: Hybridized to Axiom®_Exome_319


title: Blood_0_16

description: Hybridized to Axiom®_Exome_319


title: Blood_1_966

description: Hybridized to Axiom®_Exome_319


title: Blood_1_976

description: Hybridized to Axiom®_Exome_319


title: Blood_1_962

description: Hybridized to Axiom®_Exome_319


title: Blood_1_964

description: Hybridized to Axiom®_Exome_319


title: Blood_1_972

description: Hybridized to Axiom®_Exome_319


title: Blood_1_963

description: Hybridized to Axiom®_Exome_319


title: Blood_1_956

description: Hybridized to Axiom®_Exome_319


title: Blood_1_965

description: Hybridized to Axiom®_Exome_319


title: Blood_1_960

description: Hybridized to Axiom®_Exome_319


title: Blood_1_937

description: Hybridized to Axiom®_Exome_319


title: Blood_1_953

description: Hybridized to Axiom®_Exome_319


title: Blood_1_871

description: Hybridized to Axiom®_Exome_319


title: Blood_1_924

description: Hybridized to Axiom®_Exome_319


title: Blood_1_904

description: Hybridized to Axiom®_Exome_319


title: Blood_1_935

description: Hybridized to Axiom®_Exome_319


title: Blood_1_917

description: Hybridized to Axiom®_Exome_319


title: Blood_1_887

description: Hybridized to Axiom®_Exome_319


title: Blood_1_869

description: Hybridized to Axiom®_Exome_319


title: Blood_1_865

description: Hybridized to Axiom®_Exome_319


title: Blood_0_182

description: Hybridized to Axiom®_Exome_319


title: Blood_1_933

description: Hybridized to Axiom®_Exome_319


title: Blood_1_776

description: Hybridized to Axiom®_Exome_319


title: Blood_0_183

description: Hybridized to Axiom®_Exome_319


title: Blood_1_875

description: Hybridized to Axiom®_Exome_319


title: Blood_1_772

description: Hybridized to Axiom®_Exome_319


title: Blood_1_774

description: Hybridized to Axiom®_Exome_319


title: Blood_1_775

description: Hybridized to Axiom®_Exome_319


title: Blood_1_773

description: Hybridized to Axiom®_Exome_319


title: Blood_1_771

description: Hybridized to Axiom®_Exome_319


title: Blood_1_764

description: Hybridized to Axiom®_Exome_319


title: Blood_1_769

description: Hybridized to Axiom®_Exome_319


title: Blood_1_770

description: Hybridized to Axiom®_Exome_319


title: Blood_1_768

description: Hybridized to Axiom®_Exome_319


title: Blood_1_969

description: Hybridized to Axiom®_Exome_319


title: Blood_1_942

description: Hybridized to Axiom®_Exome_319


title: Blood_1_767

description: Hybridized to Axiom®_Exome_319


title: Blood_1_762

description: Hybridized to Axiom®_Exome_319


title: Blood_0_185

description: Hybridized to Axiom®_Exome_319


title: Blood_1_763

description: Hybridized to Axiom®_Exome_319


title: Blood_1_758

description: Hybridized to Axiom®_Exome_319


title: Blood_1_765

description: Hybridized to Axiom®_Exome_319


title: Blood_1_757

description: Hybridized to Axiom®_Exome_319


title: Blood_1_760

description: Hybridized to Axiom®_Exome_319


title: Blood_1_754

description: Hybridized to Axiom®_Exome_319


title: Blood_1_748

description: Hybridized to Axiom®_Exome_319


title: Blood_1_751

description: Hybridized to Axiom®_Exome_319


title: Blood_1_756

description: Hybridized to Axiom®_Exome_319


title: Blood_1_755

description: Hybridized to Axiom®_Exome_319


title: Blood_1_747

description: Hybridized to Axiom®_Exome_319


title: Blood_1_759

description: Hybridized to Axiom®_Exome_319


title: Blood_1_750

description: Hybridized to Axiom®_Exome_319


title: Blood_1_753

description: Hybridized to Axiom®_Exome_319


title: Blood_1_766

description: Hybridized to Axiom®_Exome_319


title: Blood_1_761

description: Hybridized to Axiom®_Exome_319


title: Blood_1_752

description: Hybridized to Axiom®_Exome_319


title: Blood_1_742

description: Hybridized to Axiom®_Exome_319


title: Blood_1_749

description: Hybridized to Axiom®_Exome_319


title: Blood_1_743

description: Hybridized to Axiom®_Exome_319


title: Blood_1_741

description: Hybridized to Axiom®_Exome_319


title: Blood_1_739

description: Hybridized to Axiom®_Exome_319


title: Blood_1_744

description: Hybridized to Axiom®_Exome_319


title: Blood_1_735

description: Hybridized to Axiom®_Exome_319


title: Blood_1_745

description: Hybridized to Axiom®_Exome_319


title: Blood_1_740

description: Hybridized to Axiom®_Exome_319


title: Blood_1_734

description: Hybridized to Axiom®_Exome_319


title: Blood_1_738

description: Hybridized to Axiom®_Exome_319


title: Blood_1_733

description: Hybridized to Axiom®_Exome_319


title: Blood_1_731

description: Hybridized to Axiom®_Exome_319


title: Blood_1_730

description: Hybridized to Axiom®_Exome_319


title: Blood_1_732

description: Hybridized to Axiom®_Exome_319


title: Blood_1_728

description: Hybridized to Axiom®_Exome_319


title: Blood_1_736

description: Hybridized to Axiom®_Exome_319


title: Blood_1_727

description: Hybridized to Axiom®_Exome_319


title: Blood_1_717

description: Hybridized to Axiom®_Exome_319


title: Blood_1_746

description: Hybridized to Axiom®_Exome_319


title: Blood_1_720

description: Hybridized to Axiom®_Exome_319


title: Blood_1_716

description: Hybridized to Axiom®_Exome_319


title: Blood_1_729

description: Hybridized to Axiom®_Exome_319


title: Blood_1_721

description: Hybridized to Axiom®_Exome_319


title: Blood_1_737

description: Hybridized to Axiom®_Exome_319


title: Blood_1_725

description: Hybridized to Axiom®_Exome_319


title: Blood_1_712

description: Hybridized to Axiom®_Exome_319


title: Blood_1_714

description: Hybridized to Axiom®_Exome_319


title: Blood_1_715

description: Hybridized to Axiom®_Exome_319


title: Blood_1_718

description: Hybridized to Axiom®_Exome_319


title: Blood_1_724

description: Hybridized to Axiom®_Exome_319


title: Blood_1_722

description: Hybridized to Axiom®_Exome_319


title: Blood_1_711

description: Hybridized to Axiom®_Exome_319


title: Blood_1_709

description: Hybridized to Axiom®_Exome_319


title: Blood_1_726

description: Hybridized to Axiom®_Exome_319


title: Blood_1_710

description: Hybridized to Axiom®_Exome_319


title: Blood_1_707

description: Hybridized to Axiom®_Exome_319


title: Blood_1_719

description: Hybridized to Axiom®_Exome_319


title: Blood_1_703

description: Hybridized to Axiom®_Exome_319


title: Blood_1_701

description: Hybridized to Axiom®_Exome_319


title: Blood_1_704

description: Hybridized to Axiom®_Exome_319


title: Blood_1_713

description: Hybridized to Axiom®_Exome_319


title: Blood_1_706

description: Hybridized to Axiom®_Exome_319


title: Blood_1_700

description: Hybridized to Axiom®_Exome_319


title: Blood_1_705

description: Hybridized to Axiom®_Exome_319


title: Blood_1_699

description: Hybridized to Axiom®_Exome_319


title: Blood_1_698

description: Hybridized to Axiom®_Exome_319


title: Blood_0_690

description: Hybridized to Axiom®_Exome_319


title: Blood_1_696

description: Hybridized to Axiom®_Exome_319


title: Blood_0_688

description: Hybridized to Axiom®_Exome_319


title: Blood_1_695

description: Hybridized to Axiom®_Exome_319


title: Blood_1_697

description: Hybridized to Axiom®_Exome_319


title: Blood_0_692

description: Hybridized to Axiom®_Exome_319


title: Blood_0_684

description: Hybridized to Axiom®_Exome_319


title: Blood_1_694

description: Hybridized to Axiom®_Exome_319


title: Blood_0_693

description: Hybridized to Axiom®_Exome_319


title: Blood_1_723

description: Hybridized to Axiom®_Exome_319


title: Blood_0_689

description: Hybridized to Axiom®_Exome_319


title: Blood_0_686

description: Hybridized to Axiom®_Exome_319


title: Blood_0_685

description: Hybridized to Axiom®_Exome_319


title: Blood_0_679

description: Hybridized to Axiom®_Exome_319


title: Blood_1_708

description: Hybridized to Axiom®_Exome_319


title: Blood_0_680

description: Hybridized to Axiom®_Exome_319


title: Blood_0_682

description: Hybridized to Axiom®_Exome_319


title: Blood_0_674

description: Hybridized to Axiom®_Exome_319


title: Blood_0_676

description: Hybridized to Axiom®_Exome_319


title: Blood_0_678

description: Hybridized to Axiom®_Exome_319


title: Blood_0_683

description: Hybridized to Axiom®_Exome_319


title: Blood_0_687

description: Hybridized to Axiom®_Exome_319


title: Blood_0_681

description: Hybridized to Axiom®_Exome_319


title: Blood_0_669

description: Hybridized to Axiom®_Exome_319


title: Blood_0_673

description: Hybridized to Axiom®_Exome_319


title: Blood_0_670

description: Hybridized to Axiom®_Exome_319


title: Blood_0_668

description: Hybridized to Axiom®_Exome_319


title: Blood_0_672

description: Hybridized to Axiom®_Exome_319


title: Blood_1_702

description: Hybridized to Axiom®_Exome_319


title: Blood_0_675

description: Hybridized to Axiom®_Exome_319


title: Blood_0_671

description: Hybridized to Axiom®_Exome_319


title: Blood_0_667

description: Hybridized to Axiom®_Exome_319


title: Blood_0_665

description: Hybridized to Axiom®_Exome_319


title: Blood_0_677

description: Hybridized to Axiom®_Exome_319


title: Blood_0_658

description: Hybridized to Axiom®_Exome_319


title: Blood_0_662

description: Hybridized to Axiom®_Exome_319


title: Blood_0_657

description: Hybridized to Axiom®_Exome_319


title: Blood_0_660

description: Hybridized to Axiom®_Exome_319


title: Blood_0_663

description: Hybridized to Axiom®_Exome_319


title: Blood_0_664

description: Hybridized to Axiom®_Exome_319


title: Blood_0_659

description: Hybridized to Axiom®_Exome_319


title: Blood_0_651

description: Hybridized to Axiom®_Exome_319


title: Blood_0_652

description: Hybridized to Axiom®_Exome_319


title: Blood_0_655

description: Hybridized to Axiom®_Exome_319


title: Blood_0_654

description: Hybridized to Axiom®_Exome_319


title: Blood_0_656

description: Hybridized to Axiom®_Exome_319


title: Blood_0_650

description: Hybridized to Axiom®_Exome_319


title: Blood_0_642

description: Hybridized to Axiom®_Exome_319


title: Blood_0_644

description: Hybridized to Axiom®_Exome_319


title: Blood_0_645

description: Hybridized to Axiom®_Exome_319


title: Blood_0_638

description: Hybridized to Axiom®_Exome_319


title: Blood_0_653

description: Hybridized to Axiom®_Exome_319


title: Blood_0_649

description: Hybridized to Axiom®_Exome_319


title: Blood_0_641

description: Hybridized to Axiom®_Exome_319


title: Blood_0_648

description: Hybridized to Axiom®_Exome_319


title: Blood_0_647

description: Hybridized to Axiom®_Exome_319


title: Blood_0_646

description: Hybridized to Axiom®_Exome_319


title: Blood_0_636

description: Hybridized to Axiom®_Exome_319


title: Blood_0_639

description: Hybridized to Axiom®_Exome_319


title: Blood_0_637

description: Hybridized to Axiom®_Exome_319


title: Blood_0_635

description: Hybridized to Axiom®_Exome_319


title: Blood_0_634

description: Hybridized to Axiom®_Exome_319


title: Blood_0_661

description: Hybridized to Axiom®_Exome_319


title: Blood_0_666

description: Hybridized to Axiom®_Exome_319


title: Blood_0_633

description: Hybridized to Axiom®_Exome_319


title: Blood_0_629

description: Hybridized to Axiom®_Exome_319


title: Blood_0_626

description: Hybridized to Axiom®_Exome_319


title: Blood_0_631

description: Hybridized to Axiom®_Exome_319


title: Blood_0_630

description: Hybridized to Axiom®_Exome_319


title: Blood_0_627

description: Hybridized to Axiom®_Exome_319


title: Blood_0_628

description: Hybridized to Axiom®_Exome_319


title: Blood_0_632

description: Hybridized to Axiom®_Exome_319


title: Blood_0_625

description: Hybridized to Axiom®_Exome_319


title: Blood_0_624

description: Hybridized to Axiom®_Exome_319


title: Blood_0_616

description: Hybridized to Axiom®_Exome_319


title: Blood_0_608

description: Hybridized to Axiom®_Exome_319


title: Blood_0_617

description: Hybridized to Axiom®_Exome_319


title: Blood_0_620

description: Hybridized to Axiom®_Exome_319


title: Blood_0_621

description: Hybridized to Axiom®_Exome_319


title: Blood_0_623

description: Hybridized to Axiom®_Exome_319


title: Blood_0_622

description: Hybridized to Axiom®_Exome_319


title: Blood_0_618

description: Hybridized to Axiom®_Exome_319


title: Blood_0_610

description: Hybridized to Axiom®_Exome_319


title: Blood_0_609

description: Hybridized to Axiom®_Exome_319


title: Blood_0_606

description: Hybridized to Axiom®_Exome_319


title: Blood_0_611

description: Hybridized to Axiom®_Exome_319


title: Blood_0_619

description: Hybridized to Axiom®_Exome_319


title: Blood_0_603

description: Hybridized to Axiom®_Exome_319


title: Blood_0_605

description: Hybridized to Axiom®_Exome_319


title: Blood_0_607

description: Hybridized to Axiom®_Exome_319


title: Blood_0_604

description: Hybridized to Axiom®_Exome_319


title: Blood_0_612

description: Hybridized to Axiom®_Exome_319


title: Blood_0_602

description: Hybridized to Axiom®_Exome_319


title: Blood_0_615

description: Hybridized to Axiom®_Exome_319


title: Blood_0_614

description: Hybridized to Axiom®_Exome_319


title: Blood_0_640

description: Hybridized to Axiom®_Exome_319


title: Blood_0_613

description: Hybridized to Axiom®_Exome_319


title: Blood_0_601

description: Hybridized to Axiom®_Exome_319


title: Blood_0_595

description: Hybridized to Axiom®_Exome_319


title: Blood_0_599

description: Hybridized to Axiom®_Exome_319


title: Blood_0_597

description: Hybridized to Axiom®_Exome_319


title: Blood_0_596

description: Hybridized to Axiom®_Exome_319


title: Blood_0_598

description: Hybridized to Axiom®_Exome_319


title: NON-UV3

city: Manchester

first: Simon

last: Furney

state: Lancashire

address: Wilmslow road

country: United Kingdom

institute: CRUKMI

description: array CGH of mouse melanoma sample

hyb_protocol: Hybridization was processing overnight at 42°C in Roche-NimbleGen hybridization system12

scan_protocol: Arrays were scanned on an Roche-NimbleGen MS200 scanner

data_processing: Arrays were processed using NimbleScan,which transformed image signal to digital signal, following using segMNT algorithm to analyse the normalized signal ratio

organism: Mus musculus

source_name: DNA from tumour from NON-UV3

label_protocol: The sample labelling was using NimbleGen Dual-Color DNA Labeling Kit

extract_protocol: DNA extraction was performed using a DNeasy blood and tissue kit (Qiagen).

tag: strain

value: C57 black 6

source_name: Germline DNA from NON-UV3


title: Blood_0_592

description: Hybridized to Axiom®_Exome_319


title: UV14

description: array CGH of mouse melanoma sample

source_name: DNA from tumour from UV14

source_name: Germline DNA from UV14


title: Blood_0_594

description: Hybridized to Axiom®_Exome_319


title: UV6

description: array CGH of mouse melanoma sample

source_name: DNA from tumour from UV6

source_name: Germline DNA from UV6


title: Blood_0_591

description: Hybridized to Axiom®_Exome_319


title: Blood_0_588

description: Hybridized to Axiom®_Exome_319


title: Blood_0_589

description: Hybridized to Axiom®_Exome_319


title: Blood_0_593

description: Hybridized to Axiom®_Exome_319


title: Blood_0_590

description: Hybridized to Axiom®_Exome_319


title: UV5

description: array CGH of mouse melanoma sample

source_name: DNA from tumour from UV5

source_name: Germline DNA from UV5


title: Blood_0_587

description: Hybridized to Axiom®_Exome_319


title: UV11

description: array CGH of mouse melanoma sample

source_name: DNA from tumour from UV11

source_name: Germline DNA from UV11


title: UV15

description: array CGH of mouse melanoma sample

source_name: DNA from tumour from UV15

source_name: Germline DNA from UV15


title: UV2

description: array CGH of mouse melanoma sample

source_name: DNA from tumour from UV2

source_name: Germline DNA from UV2


title: NON-UV5

description: array CGH of mouse melanoma sample

source_name: DNA from tumour from NON-UV5

source_name: Germline DNA from NON-UV5


title: UV3

description: array CGH of mouse melanoma sample

source_name: DNA from tumour from UV3

source_name: Germline DNA from UV3


title: Blood_0_586

description: Hybridized to Axiom®_Exome_319

source_name: peripheral blood leukocytes_control


title: NON-UV15

description: array CGH of mouse melanoma sample

source_name: DNA from tumour from NON-UV15

source_name: Germline DNA from NON-UV15


title: UV1

description: array CGH of mouse melanoma sample

source_name: DNA from tumour from UV1

source_name: Germline DNA from UV1


title: UV7

description: array CGH of mouse melanoma sample

source_name: DNA from tumour from UV7

source_name: Germline DNA from UV7


title: NON-UV11

description: array CGH of mouse melanoma sample

source_name: DNA from tumour from NON-UV11

source_name: Germline DNA from NON-UV11


title: NON-UV13

description: array CGH of mouse melanoma sample

source_name: DNA from tumour from NON-UV13

source_name: Germline DNA from NON-UV13


title: NON-UV1

description: array CGH of mouse melanoma sample

source_name: DNA from tumour from NON-UV1

source_name: Germline DNA from NON-UV1


title: UV9

description: array CGH of mouse melanoma sample

source_name: DNA from tumour from UV9

source_name: Germline DNA from UV9


title: NON-UV4

description: array CGH of mouse melanoma sample

source_name: DNA from tumour from NON-UV4

source_name: Germline DNA from NON-UV4


title: UV10

description: array CGH of mouse melanoma sample

source_name: DNA from tumour from UV10

source_name: Germline DNA from UV10


title: NON-UV2

description: array CGH of mouse melanoma sample

source_name: DNA from tumour from NON-UV2

source_name: Germline DNA from NON-UV2


title: Blood_0_584

description: Hybridized to Axiom®_Exome_319

source_name: peripheral blood leukocytes_control


title: Blood_0_583

description: Hybridized to Axiom®_Exome_319


title: Blood_0_582

description: Hybridized to Axiom®_Exome_319


title: serum + spiked miRs variable concentration repeat

status: Public on Jun 10 2014

type: RNA

city: Santa Clara

first: Stephanie

last: Fulmer-Smentek

state: CA

address: 5301 Stevens Creek Blvd

department: R&D

country: USA

institute: Agilent Technologies

description: SAMPLE 20

hyb_protocol: Hybridization was performed according to the Agilent miRNA microarray system with miRNA complete labeling and hyb kit protocol v.2.4 (Agilent publication number G4170-90011).  A hybridization solution for each of the of the labeled samples was prepared using the components of the gene expression hybridization kit (PN5188-5242) and were hybridized to Agilent human microRNA microarrays based on the miRBase v 16.0 release (microarray design ID 031181; PN G4870A).  The microarrays were hybridized rotating in a 55 °C oven for 20 hours.  After hybridization the microarrays were disassembled and washed in the Agilent gene expression wash buffer 1 at room temperature for 5 minutes and then washed in pre-heated Agilent gene expression wash buffer 2 (Agilent gene expression wash buffer kit; PN 5188-5327) at 37 °C for another 5 minutes.

scan_protocol: The dried microarray slides were scanned using the Agilent microarray scanner system.

data_processing:
The data captured on the scanned images were extracted using the Agilent feature extraction software.  The Feature Extraction files were loaded into GeneSpring GX software where the data was log2 transformed and normalized using 90 percentile shift.
Series supplementary file "miRQC_Agilent_DataMatrix" values are log 2 transformed and 90th percentile shift normalized intensity values provided per miRNA

molecule: total RNA

source_name: Serum isolated RNA spiked with miR-10a-5p, let-7a-5p, miR-302a-3p and miR-133a (variable concentration)

label_protocol: Labeling was performed according to the Agilent miRNA microarray system with miRNA complete labeling and hyb kit protocol v.2.4 (Agilent publication number G4170-90011).  Each 100 ng (2 µl, 50 ng/µl) sample was labeled utilizing components from the Agilent miRNA complete labeling and hyb kit (PN 5190-0456).  For the serum samples, 10 µl RNA was dried and re-suspended in 2 µl of nuclease-free water.  Once re-suspended the serum samples were labeled in accordance to the protocol just as the 100 ng total RNA samples.

extract_protocol: Universal Human miRNA reference RNA (Agilent Technologies, #750700), human brain total RNA (Life Technologies, #AM6050), human liver total RNA (Life Technologies, # AM7960) and MS2 phage RNA (Roche, #10165948001) were diluted to a platform-specific concentration. RNA was isolated from serum prepared from 3 healthy donors using the miRNeasy mini kit (Qiagen) according to the manufacturer’s instructions and RNA samples were pooled. Synthetic miRNA templates for let-7a-5p, let-7b-5p, let-7c, let-7d-5p, miR-302a-3p, miR-302b-3p, miR-302c-3p, miR-302d-3p, miR-133a and miR-10a-5p were synthesized by Integrated DNA Technologies and 5’ phosphorylated. Synthetic let-7 and miR-302 miRNAs were spiked in MS2 phage RNA and total human liver RNA respectively at 5 x 106 copies / µg RNA. Synthetic miRNA templates for miR-10a-5p, let-7a-5p, miR-302a-3p and miR-133a were spiked in serum RNA at 6 x 103 copies per µl serum RNA or at 5-times higher, 2-times higher, 2-times lower and 5-times lower concentrations respectively. All vendors received 10 µl of each serum RNA sample.

tag: source


title: serum + spiked miRs variable concentration

description: SAMPLE 19

value: Serum isolated RNA spiked with miR-10a-5p, let-7a-5p, miR-302a-3p and miR-133a (variable concentration)


title: Blood_0_581

description: Hybridized to Axiom®_Exome_319


title: Blood_0_585

description: Hybridized to Axiom®_Exome_319


title: serum + spiked miRs constant concentration repeat

description: SAMPLE 18

source_name: Serum isolated RNA spiked with miR-10a-5p, let-7a-5p, miR-302a-3p and miR-133a (6 x 10^3 copies per µl serum RNA)


title: Male diet C liver tissue, biological rep2

city: Riyadh

first: Marya

middle: Zia

last: Zaidi

state: Riyadh

address: 1 Takhassussi Road

department: Biological & Medical Research

country: Saudi Arabia

institute: KFSH&RC

description:
CAT# 8_(HuGene-1_0-st-v1)
Gene expression data from 9-month-old male diet C liver.

hyb_protocol: Following fragmentation, 5.5 ug of ssDNA was hybridized for 17 hr at 45°C on GeneChip(R) Human Gene 1.0 ST arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.

scan_protocol: GeneChips were scanned using the Affymetrix 3000 7G Scanner, and GeneChip Operating Software version 1.4  was used to produce .CEL files.

data_processing:
Microarray analysis was performed using the Partek genomic suite software version 6.4 (Partek, MO, USA).  We imported the interrogating probes using the file HuGene-1_0-st-v1.na31.hg19.probeset.csv.zip downloaded from: http://www.affymetrix.com/estore/browse/products.jsp?productId=131453&categoryId=35676&productName=GeneChip-Human-Gene-1.0-ST-Array#1_3
Probe set data were summarized, background adjusted, and quantile normalized using the GC-Robust Multi-Array (GCRMA) algorithm, no log. A pre-background adjustment for probe sequence was also applied.

label: biotin

organism: Felis catus

source_name: liver, diet C

label_protocol: Biotinylated ssDNA were prepared according to the standard Affymetrix protocol from 250ng total RNA (GeneChip® Whole Transcript (WT) Sense Target Labeling Assay Technical Manual, 2009, Affymetrix).

growth_protocol: Our study animals were bred from female Felis catus previously placed on one of 4 different dietary regimens for a period of 3 weeks prior to mating. The four dietary regimens used in this study were: [1] Standard Chow Control feline diet (Test Diet Purina catalog #5003); [2] MSG diet consisting of Control diet with 1.125% added Monosodium Glutamate (Diet A: Test Diet Purina catalog #5C1J); [3] Trans-fat/HFCS diet, containing  8.6% Trans fat and 24% HFCS (Diet B: Test Diet Purina catalog #5B4K); and [4] Trans-fat/HFCS and MSG diet, containing 8.6% Trans fat, 24% HFCS and 1.125% MSG (Diet C: Test Diet Purina catalog #5C1H). Following mating, the 4 groups of dams were maintained on their respective diets throughout the gestation and nursing period.

extract_protocol: Hepatic tissues (4-5 per diet group) were used for RNA extraction. Total RNA was prepared from snap-frozen liver tissue using the QIAGEN RNeasy® Kit according to the manufacturer's instructions. The integrity of the RNA was measured using a 2100 Bioanalyzer instrument and an RNA 6000 Nano LabChip assay (Agilent Technologies).

treatment_protocol: Male offspring used in the following experiments were weaned onto the same diets as the dams and were maintained on their respective dietary regimens until they reached 9 months of age. The four dietary regimens used in this study were: [1] Standard Chow Control feline diet (Test Diet Purina catalog #5003); [2] MSG diet consisting of Control diet with 1.125% added Monosodium Glutamate (Diet A: Test Diet Purina catalog #5C1J); [3] Trans-fat/HFCS diet, containing  8.6% Trans fat and 24% HFCS (Diet B: Test Diet Purina catalog #5B4K); and [4] Trans-fat/HFCS and MSG diet, containing 8.6% Trans fat, 24% HFCS and 1.125% MSG (Diet C: Test Diet Purina catalog #5C1H).

tag: age

value: 9 months

tag: tissue

value: liver

tag: treatment

value: trans-fat, HFCS, and MSG diet


title: Male diet A liver tissue, biological rep2

description:
CAT# 4_(HuGene-1_0-st-v1)
Gene expression data from 9-month-old male diet A liver.

source_name: liver, diet A

value: MSG diet


title: Male diet A liver tissue, biological rep1

description:
CAT# 3_(HuGene-1_0-st-v1)
Gene expression data from 9-month-old male diet A liver.


title: NON-UV6

description: array CGH of mouse melanoma sample

source_name: DNA from tumour from NON-UV6

source_name: Germline DNA from NON-UV6


title: UV13

description: array CGH of mouse melanoma sample

source_name: DNA from tumour from UV13

source_name: Germline DNA from UV13


title: MS2 + let-7c

description: SAMPLE 15

organism: Escherichia phage MS2

source_name: MS2 phage RNA spiked with Let7c (5 x 10^6 copies / µg RNA )


title: Male diet C liver tissue, biological rep1

description:
CAT# 7_(HuGene-1_0-st-v1)
Gene expression data from 9-month-old male diet C liver.


title: MS2 + let-7a-5p

description: SAMPLE 13

source_name: MS2 phage RNA spiked with Let7a-5p (5 x 10^6 copies / µg RNA )


title: Male diet B liver tissue, biological rep2

description:
CAT# 6_(HuGene-1_0-st-v1)
Gene expression data from 9-month-old male diet B liver.

source_name: liver, diet B

value: trans-fat and HFCS diet


title: serum + spiked miRs constant concentration

description: SAMPLE 17

value: Serum isolated RNA spiked with miR-10a-5p, let-7a-5p, miR-302a-3p and miR-133a (6 x 10^3 copies per µl serum RNA)


title: Male control diet liver tissue, biological rep2

description:
CAT# 2_(HuGene-1_0-st-v1)
Gene expression data from 9-month-old male control diet liver.

source_name: liver, control diet

value: control diet


title: liver + miR-302c

description: SAMPLE 11

source_name: Total Liver RNA spiked with miR302c (5 x 10^6 copies / µg RNA)


title: Male control diet liver tissue, biological rep1

description:
CAT# 1_(HuGene-1_0-st-v1)
Gene expression data from 9-month-old male control diet liver.


title: liver + miR-302b

description: SAMPLE 10

source_name: Total Liver RNA spiked with miR302b (5 x 10^6 copies / µg RNA)


title: liver + miR-302d

description: SAMPLE 12

source_name: Total Liver RNA spiked with miR302d (5 x 10^6 copies / µg RNA)


title: liver + miR-302a

description: SAMPLE 9

source_name: Total Liver RNA spiked with miR302a (5 x 10^6 copies / µg RNA)


title: MS2 + let-7b-5p

description: SAMPLE 14

source_name: MS2 phage RNA spiked with Let7b-5p (5 x 10^6 copies / µg RNA )


title: miRQC D repeat

description: SAMPLE 8

source_name: 25% Universal Human Reference RNA+75% Human Brain RNA


title: miRQC D

description: SAMPLE 7

value: 25% Universal Human Reference RNA+75% Human Brain RNA


title: UV4

description: array CGH of mouse melanoma sample

source_name: DNA from tumour from UV4

source_name: Germline DNA from UV4


title: miRQC C repeat

description: SAMPLE 6

source_name: 75% Universal Human miRNA Reference RNA+ 25% Human Brain RNA


title: Male diet B liver tissue, biological rep1

description:
CAT# 5_(HuGene-1_0-st-v1)
Gene expression data from 9-month-old male diet B liver.


title: UV12

description: array CGH of mouse melanoma sample

source_name: DNA from tumour from UV12

source_name: Germline DNA from UV12


title: Blood_0_577

description: Hybridized to Axiom®_Exome_319

source_name: peripheral blood leukocytes_control


title: miRQC C

description: SAMPLE 5

value: 75% Universal Human miRNA Reference RNA+ 25% Human Brain RNA


title: miRQC B

description: SAMPLE 3

source_name: human brain RNA

value: human brain RNA


title: NON-UV9

description: array CGH of mouse melanoma sample

source_name: DNA from tumour from NON-UV9

source_name: Germline DNA from NON-UV9


title: miRQC A

description: SAMPLE 1

source_name: universal human miRNA reference RNA

value: universal human miRNA reference RNA


title: miRQC A repeat

description: SAMPLE 2


title: Blood_0_578

description: Hybridized to Axiom®_Exome_319

value: control


title: Blood_0_579

description: Hybridized to Axiom®_Exome_319


title: NON-UV14

description: array CGH of mouse melanoma sample

source_name: DNA from tumour from NON-UV14

source_name: Germline DNA from NON-UV14


title: MS2 + let-7d-5p

description: SAMPLE 16

source_name: MS2 phage RNA spiked with Let7d-5p (5 x 10^6 copies / µg RNA )


title: Blood_0_580

description: Hybridized to Axiom®_Exome_319


title: NON-UV10

description: array CGH of mouse melanoma sample

source_name: DNA from tumour from NON-UV10

source_name: Germline DNA from NON-UV10


title: leukocytes_human 47 (IV)

city: Moscow

first: Anastasia

last: Shindyapina

state: Moscow

address: Mosfilmovskaya 76

department: Biengineering

country: Russia

institute: MSU Lomonosov

description: SAMPLE 47

hyb_protocol: Standard Illumina hybridization protocol

scan_protocol: Standard Illumina scanning protocol

data_processing: The data were normalized using quantile normalization with J-Express 2012

source_name: human leukocytes

label_protocol: Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays

extract_protocol: RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.

treatment_protocol: The subjects fasted for 12 hours prior to beginning each experiment and evaluation session. Each volunteer swallowed capsules with PME containing citrus pectin (6 g) (Nittary Pharmaceuticals, VitaLine, Inc., USA). After 120 min blood samples were obtained. Total RNA was isolated from WBCs with TriReagent (MRC, USA) according to the manufacturer’s protocol.

tag: agent

tag: cell type

value: WBC


title: leukocytes_human_pectin_2h 57 (IV)

description: SAMPLE 57

source_name: human leukocytes, pectin, 2 h

value: male

value: pectin


title: leukocytes_human_pectin_2h 55 (IV)

description: SAMPLE 55


title: leukocytes_human_pectin_2h 56 (IV)

description: SAMPLE 56

value: female


title: leukocytes_human 43 (IV)

description: SAMPLE 43


title: leukocytes_human_pectin_2h 52 (IV)

description: SAMPLE 52


title: leukocytes_human_pectin_2h 54 (IV)

description: SAMPLE 54


title: leukocytes_human 46 (IV)

description: SAMPLE 46


title: leukocytes_human_pectin_2h 53 (IV)

description: SAMPLE 53


title: leukocytes_human 44 (IV)

description: SAMPLE 44


title: leukocytes_human 45 (IV)

description: SAMPLE 45


title: leukocytes_human_pectin_2h 8.2 (II)

description: SAMPLE 8.2

tag: individual


title: leukocytes_human_pectin_2h 5.2 (II)

description: SAMPLE 5.2


title: leukocytes_human_pectin_2h 3.2 (II)

description: SAMPLE 3.2


title: NON-UV7

description: array CGH of mouse melanoma sample

source_name: DNA from tumour from NON-UV7

value: C57 black 6

source_name: Germline DNA from NON-UV7


title: leukocytes_human_pectin_2h 4.2 (II)

description: SAMPLE 4.2


title: leukocytes_human 42 (IV)

description: SAMPLE 42


title: Blood_1_1022

description: Hybridized to Axiom®_Exome_319

source_name: peripheral blood leukocytes_case

value: gastric cancer case


title: NON-UV12

description: array CGH of mouse melanoma sample

source_name: DNA from tumour from NON-UV12

source_name: Germline DNA from NON-UV12


title: leukocytes_human 8.0 (II)

description: SAMPLE 8.0


title: leukocytes_human 4.0 (II)

description: SAMPLE 4.0


title: leukocytes_human_pectin_2h 1.2 (II)

description: SAMPLE 1.2


title: leukocytes_human 3.0 (II)

description: SAMPLE 3.0


title: UV8

description: array CGH of mouse melanoma sample

source_name: DNA from tumour from UV8

source_name: Germline DNA from UV8


title: leukocytes_human_pectin_2h 7.2 (II)

description: SAMPLE 7.2


title: leukocytes_human_pectin_2h 7B (I)

description: SAMPLE 7B

value: volunteer 7


title: leukocytes_human 1.0 (II)

description: SAMPLE 1.0


title: leukocytes_human_pectin_2h 4B (I)

description: SAMPLE 4B

value: volunteer 4


title: leukocytes_human_pectin_2h 6B (I)

description: SAMPLE 6B

value: volunteer 6


title: leukocytes_human 6A (I)

description: SAMPLE 6A


title: leukocytes_human 5.0 (II)

description: SAMPLE 5.0


title: leukocytes_human 7.0 (II)

description: SAMPLE 7.0


title: leukocytes_human_pectin_2h 1B (I)

description: SAMPLE 1B

value: volunteer 1


title: leukocytes_human 2A (I)

description: SAMPLE 2A

value: volunteer 2


title: leukocytes_human_pectin_2h 2B (I)

description: SAMPLE 2B


title: leukocytes_human 4A (I)

description: SAMPLE 4A


title: NON-UV8

description: array CGH of mouse melanoma sample

source_name: DNA from tumour from NON-UV8

source_name: Germline DNA from NON-UV8


title: miRQC B repeat

description: SAMPLE 4

source_name: human brain RNA


title: leukocytes_human 3A (I)

description: SAMPLE 3A

value: volunteer 3


title: leukocytes_human 7A (I)

description: SAMPLE 7A


title: leukocytes_human 1A (I)

description: SAMPLE 1A


title: leukocytes_human_pectin_2h 3B (I)

description: SAMPLE 3B


title: ovarian clear cell carcinoma 144

city: Tokyo

first: Mitsuyoshi

last: Urashima

address: Nishishimbashi 3-25-8, Minato-ku

country: Japan

institute: Jikei University School of Medicine

hyb_protocol: Scanned on an Agilent G4449A scanner.

scan_protocol: Images were quantified using Agilent Feature Extraction Software (version A.8.5.1.1).

data_processing: Agilent Feature Extraction Software (v 8.5.1.1) was used for background subtraction and LOWESS normalization.

source_name: cancer patient

label_protocol: following manufacturer's instructions

growth_protocol: Clinical samples

extract_protocol: 0.5 µg of total DNA were primed following manufacturer's instructions

treatment_protocol: Total DNA by out sorcing

tag: disease state

value: ovarian clear cell carcinoma

tag: population (japanese=1, german=0, korean=2)

tag: endometriosis (presence=1; absence=0)

tag: thrombosis (presence=1; absence=0)

tag: sensitivity to chemotherapy (sensitive=2; intermediate=1; resistant=0)

tag: figo stage

tag: relapse (relapsed=1; not relapsed =0)

tag: death (death=1; survive=0)

source_name: normal, healthy volunteer

value: peripheral blood

tag: reference composition

value: human mixed control


title: ovarian clear cell carcinoma 143


title: ovarian clear cell carcinoma 140


title: ovarian clear cell carcinoma 142


title: ovarian clear cell carcinoma 129


title: ovarian clear cell carcinoma 128


title: ovarian clear cell carcinoma 131


title: ovarian clear cell carcinoma 137


title: ovarian clear cell carcinoma 122


title: ovarian clear cell carcinoma 138


title: ovarian clear cell carcinoma 139


title: ovarian clear cell carcinoma 132


title: ovarian clear cell carcinoma 135


title: ovarian clear cell carcinoma 111


title: ovarian clear cell carcinoma 141


title: ovarian clear cell carcinoma 134


title: ovarian clear cell carcinoma 112


title: ovarian clear cell carcinoma 130


title: ovarian clear cell carcinoma 110


title: ovarian clear cell carcinoma 127


title: ovarian clear cell carcinoma 115


title: ovarian clear cell carcinoma 119


title: ovarian clear cell carcinoma 136


title: ovarian clear cell carcinoma 124


title: ovarian clear cell carcinoma 116


title: ovarian clear cell carcinoma 113


title: ovarian clear cell carcinoma 117


title: ovarian clear cell carcinoma 123


title: ovarian clear cell carcinoma 125


title: ovarian clear cell carcinoma 105


title: ovarian clear cell carcinoma 101


title: ovarian clear cell carcinoma 100


title: ovarian clear cell carcinoma 103


title: ovarian clear cell carcinoma 126


title: ovarian clear cell carcinoma 107


title: ovarian clear cell carcinoma 97


title: ovarian clear cell carcinoma 96


title: ovarian clear cell carcinoma 95


title: ovarian clear cell carcinoma 93


title: ovarian clear cell carcinoma 109


title: ovarian clear cell carcinoma 89


title: ovarian clear cell carcinoma 94


title: ovarian clear cell carcinoma 84


title: ovarian clear cell carcinoma 88


title: ovarian clear cell carcinoma 87


title: ovarian clear cell carcinoma 85


title: ovarian clear cell carcinoma 83


title: ovarian clear cell carcinoma 82


title: ovarian clear cell carcinoma 81


title: ovarian clear cell carcinoma 77


title: ovarian clear cell carcinoma 78


title: ovarian clear cell carcinoma 90


title: PAX_Psoriasis_025

city: Hellerup

first: Marianne

middle: Bengtson

last: Løvendorf

address: Niels Andersens Vej 65

department: Dermato-Allergology

country: Denmark

institute: Gentofte Hospital

hyb_protocol: The Hy3™-labeled samples was hybridized to the miRCURY™ LNA Array version 5th Generation (Exiqon, Denmark), which contains capture probes targeting all miRNAs for human, mouse or rat registered in the miRBASE version 15.0 at the Sanger Institute. The hybridization was performed according to the miRCURY™ LNA array manual using a Tecan HS4800 hybridization station (Tecan, Austria).

scan_protocol: The miRCURY™ LNA array microarray slides were scanned using the Agilent G2565BA Microarray Scanner System (Agilent Technologies, Inc., USA) and the image analysis was carried out using the ImaGene 9.0 software (BioDiscovery, Inc., USA).

data_processing: The quantified signals were background corrected (Normexp with offset value 10 – Ritchie et al., Bioinformatics; 23: 2700-2707, 2007) and normalized using the Quantile algorithm.

source_name: Whole blood

label_protocol: 500 ng total RNA from sample was labelled with Hy3™ fluorescent label using the miRCURY™ LNA Array power labelling kit (Exiqon, Denmark) following the procedure described by the manufacturer.

extract_protocol: Total RNA was isolated using the PAXgene blood miRNA kit (PreAnalytiX/Qiagen, Copenhagen, Denmark) following the manufacturer’s instructions. The quality of the total RNA was verified by an Agilent 2100 Bioanalyzer profile.

value: Whole blood

tag: disease

value: Psoriasis

tag: gender

value: M

tag: ethnicity

value: Caucasian


title: ovarian clear cell carcinoma 76

tag: age

tag: tissue


title: ovarian clear cell carcinoma 86


title: ovarian clear cell carcinoma 73


title: ovarian clear cell carcinoma 79


title: PAX_Psoriasis_023


title: PAX_Psoriasis_016

value: F


title: ovarian clear cell carcinoma 74


title: ovarian clear cell carcinoma 71


title: PAX_Psoriasis_015


title: PAX_Psoriasis_014


title: ovarian clear cell carcinoma 69


title: ovarian clear cell carcinoma 108


title: PAX_Psoriasis_012


title: PAX_Psoriasis_009


title: ovarian clear cell carcinoma 70


title: PAX_Psoriasis_008


title: PAX_Psoriasis_007


title: ovarian clear cell carcinoma 106


title: PAX_Psoriasis_005


title: ovarian clear cell carcinoma 65


title: PAX_Psoriasis_010


title: PAX_Psoriasis_002


title: ovarian clear cell carcinoma 92


title: PAX_Psoriasis_006


title: ovarian clear cell carcinoma 66


title: PAX_Psoriasis_003


title: PAX_Psoriasis_013


title: PAX_Psoriasis_011


title: PAX_healthy_015

value: Healthy Control


title: PAX_healthy_016


title: ovarian clear cell carcinoma 72


title: ovarian clear cell carcinoma 67


title: PAX_healthy_014


title: ovarian clear cell carcinoma 64


title: ovarian clear cell carcinoma 60


title: PAX_healthy_019


title: PAX_healthy_012


title: PAX_healthy_013


title: PAX_healthy_011


title: PAX_healthy_006


title: PAX_healthy_007


title: PAX_healthy_008


title: PAX_healthy_002


title: PAX_healthy_003


title: ovarian clear cell carcinoma 63


title: PAX_Psoriasis_001


title: PAX_healthy_010


title: PAX_healthy_017


title: ovarian clear cell carcinoma 75


title: PAX_Psoriasis_017


title: ovarian clear cell carcinoma 57


title: PAX_Psoriasis_004


title: ovarian clear cell carcinoma 55


title: ovarian clear cell carcinoma 56


title: ovarian clear cell carcinoma 54


title: ovarian clear cell carcinoma 50


title: ovarian clear cell carcinoma 61


title: ovarian clear cell carcinoma 53


title: PAX_healthy_009


title: ovarian clear cell carcinoma 49


title: ovarian clear cell carcinoma 52


title: ovarian clear cell carcinoma 48


title: ovarian clear cell carcinoma 51


title: ovarian clear cell carcinoma 37


title: ovarian clear cell carcinoma 45


title: ovarian clear cell carcinoma 44


title: ovarian clear cell carcinoma 46


title: ovarian clear cell carcinoma 38


title: ovarian clear cell carcinoma 36


title: ovarian clear cell carcinoma 47


title: ovarian clear cell carcinoma 40


title: ovarian clear cell carcinoma 34


title: ovarian clear cell carcinoma 39


title: ovarian clear cell carcinoma 25


title: ovarian clear cell carcinoma 33


title: ovarian clear cell carcinoma 28


title: ovarian clear cell carcinoma 29


title: ovarian clear cell carcinoma 20


title: ovarian clear cell carcinoma 19


title: ovarian clear cell carcinoma 27


title: ovarian clear cell carcinoma 24


title: ovarian clear cell carcinoma 26


title: T lymphoblastic pleural effusion_diagnosis [TLBL_28]

city: New York

first: Riccardo

last: Dalla-Favera

state: NY

address: 1130 St Nicholas Avenue

institute: Columbia University

hyb_protocol: Hybridization mix was prepared using the Human miRNA Microarray kit (Agilent Technologies) following the manufacturer's instructions. Hybridization was performed at 55C for 20h. Washing was performed using the Gene Expression Wash Buffer kit (Agilent Technologies) following the manufacturer's instructions.

scan_protocol: Scanning was performed by the Agilent Microarray Scanner and the Agilent's Scan Control Software v. A.7.0.1 (Agilent Technologies) using the XDR feature and the recommended parameter settings.

data_processing: Raw image data were processed by the Feature Extraction software 9.5.1 (Agilent Technologies).

label_protocol: 100 ng of RNA were labeled using the Human miRNA Microarray kit (Agilent Technologies) following the manufacturer's instructions.

growth_protocol: Tissue or cells were lysated in Trizol Reagent (Invitrogen).

extract_protocol: Total RNA was extracted using Trizol Reagent (Invitrogen) following the manufacturer's instructions.

value: pleural effusion

tag: diagnosis

value: lymphoblastic tumor pleural effusion


title: T lymphoblastic tumor biopsy_diagnosis [TLBL_22]

source_name: lymphoblastic tumor biopsy_diagnosis

value: nodal tumor

value: lymphoblastic tumor biopsy


title: ovarian clear cell carcinoma 21


title: T lymphoblastic tumor biopsy_diagnosis [TLBL_18]


title: ovarian clear cell carcinoma 22


title: ovarian clear cell carcinoma 11


title: ovarian clear cell carcinoma 17


title: ovarian clear cell carcinoma 3


title: ovarian clear cell carcinoma 18


title: ovarian clear cell carcinoma 15


title: ovarian clear cell carcinoma 14


title: ovarian clear cell carcinoma 9


title: ovarian clear cell carcinoma 8


title: ovarian clear cell carcinoma 4


title: ovarian clear cell carcinoma 10


title: ovarian clear cell carcinoma 2


title: T lymphoblastic tumor biopsy_diagnosis [TLBL_12]


title: T lymphoblastic tumor biopsy_diagnosis [TLBL_13]


title: ovarian clear cell carcinoma 7


title: T lymphoblastic tumor biopsy_diagnosis [TLBL_07]


title: T lymphoblastic pleural effusion_diagnosis [TLBL_26]


title: T lymphoblastic tumor biopsy_diagnosis [TLBL_10]


title: T lymphoblastic tumor biopsy_diagnosis [TLBL_08]


title: T lymphoblastic pleural effusion_diagnosis [TLBL_27]


title: T lymphoblastic tumor biopsy_diagnosis [TLBL_05]


title: T lymphoblastic tumor biopsy_diagnosis [TLBL_04]


title: ovarian clear cell carcinoma 1


title: ovarian clear cell carcinoma 6


title: COVA

city: Goettingen

first: Gabriela

last: Salinas

state: Lower-Saxony

address: Kreuzbergring 57

department: Department of Pathology

country: Germany

institute: Universitaetsmedizin Goettingen

hyb_protocol: Hybridization oven Affymetrix; 45° 17 hs; Wash and stain Fluidic Station 450.

scan_protocol: GeneChip® Scanner 3000 7G.

data_processing: Data were quantile normalized and log2 transformed using R and Bioconductor package limma and partek.

organism: Macaca fascicularis

source_name: brain, non-infected control

label_protocol: Biotinylated cRNA was generated from 100 ng of total RNA with WT Sense Target Labeling and Control Reagent (Affymetrix).

growth_protocol: Animals were housed at the German Primate Center.

extract_protocol: Trizol extraction of total RNA was performed according to the manufacturer's instructions.

treatment_protocol: Animals were inoculated with BSE-brain homogenate intracerebrally (A1-A6), orally (B6) or were not inoculated (CovA-D2).

tag: genotype/variation

value: WT

value: 6 years

tag: Sex

value: female

value: brain

tag: infection


title: A6

source_name: brain, BSE-infected intracerebrally

value: bovine spongiform encephalopathy (BSE)-infected intracerebrally


title: BAM8_Ox, untreated, biological rep2

city: Tuebingen

first: Dierk

last: Wanke

address: Auf der Morgenstelle 32

department: Plant Physiology

institute: University of Tuebingen

description:
BAM8_Ox_B
Gene expression data from 3-week-old shoots; untreated plants.

hyb_protocol: After fragmentation of cRNA using the RNA Fragmentation Reagents (Ambion), 13.5 µg of cRNA was hybridized to Affymetrix Arabidopsis ATH1 Genome Array (ATH1-121501) according to manufacturer's manual. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the protocol 'EukGE-WS2V4'.

scan_protocol: GeneChips were scanned using the Affymetrix GeneChip® scanner GS3000 7G according to manufacturer's manual.

data_processing: The data was analysed with GeneSpring GX (vers. 7.3) from Agilent. Preprocessing was performed with included RMA Plugin.

organism: Arabidopsis thaliana

source_name: shoots, BAM8_Ox, untreated

label_protocol: 1µg of total RNA were used to synthesize amplified labeled cRNA (MessageAmp™ II aRNA Biotin Amplification Kit; Ambion).

growth_protocol: Arabidopsis thaliana (accession Columbia) were grown in a nutrient-rich, medium-grade, peat-based compost in Percival AR95 growth chambers (CLF Plant Climatics) at a constant temperature of 20°C, 70% humidity and a 12-h light period with constant, uniform light intensity of 150 μmol•m-2•s-1.

extract_protocol: For RNA extraction, RNeasy Plant Mini kit was used (according to instructions from Qiagen). Extracted RNA was precipitated ?ul RNA;  8 µl 3 M Sodium acetate (pH 5.2); 2 µl Glycogen(5mg/ml); -80°C? how long? than 30 min 13000 rpm 4°C. 2 times washing with 70% ethanol. resuspended in DEPC water. RNA quantity and integrity were assessed using RNA 6000 Nano LabChip Kit and the BioAnalyzer software (Agilent).

treatment_protocol: No treatment was applied.

tag: ecotype

value: shoot

value: 3 weeks

tag: treatment


title: BAM8_Ox, untreated, biological rep1

description:
BAM8_Ox_A
Gene expression data from 3-week-old shoots; untreated plants.


title: BAM8-N_Ox, untreated, biological rep2

description:
BAM8-N_Ox_B
Gene expression data from 3-week-old shoots; untreated plants.

source_name: shoots, BAM8-N_Ox, untreated


title: BAM8-N_Ox, untreated, biological rep1

description:
BAM8-N_Ox_A
Gene expression data from 3-week-old shoots; untreated plants.


title: B6

source_name: brain, BSE-infected orally

value: bovine spongiform encephalopathy (BSE)-infected orally


title: A2


title: BAM7_Ox, untreated, biological rep1

description:
BAM7_Ox_A
Gene expression data from 3-week-old shoots; untreated plants.

source_name: shoots, BAM7_Ox, untreated


title: COVC


title: A3


title: BAM7_Ox, untreated, biological rep2

description:
BAM7_Ox_B
Gene expression data from 3-week-old shoots; untreated plants.


title: BAM7-N_Ox, untreated, biological rep2

description:
BAM7-N_Ox_B
Gene expression data from 3-week-old shoots; untreated plants.

source_name: shoots, BAM7-N_Ox, untreated


title: BAM7-N_Ox, untreated, biological rep1

description:
BAM7-N_Ox_A
Gene expression data from 3-week-old shoots; untreated plants.


title: A1


title: A4


title: COVD2


title: A5


title: COVD1


title: COVB


title: adoptive transfer depleted BM-2

city: Milwaukee

first: Hartmut

last: Weiler

state: WI

address: 8727 Watertown Plank Road

department: Blood Research Institute

institute: BloodCenter of Wisconsin

description: heterozygous Leiden carriers with hematopoietic EPCR deficiency  were adoptively transferred with 10exp6 CD11c-POS splenocytes plus 10exp6 bone marrow cells depleted by FACS of (EPCR-F4/80)-POS cells. One day later, mice were infected intraperitoneally with S.aureus. & days later,  (Gr1-CD11b)-POS bone marrow cells were isolated by FACS and total RNA was prepared for expression analysis

hyb_protocol: fragmented, biotin-labelled cRNA was hybridized to The GeneChip Mouse Genome 430 2.0 Array in accordance to standard Affymetrix protocols (Affymetrix,  Santa Clara,  CA)

scan_protocol: After hybridization, arrays were washed and stained with Affymetrix fluidics protocol FS450_0001 and scanned with a 7G Affymetrix GeneChip Scanner

data_processing: Image data were analyzed with Affymetrix Expression Console™ software and normalized with Robust Multichip Analysis (RMA; www.bioconductor.org/)  to determine signal log ratios. The mean fold change was calculated from the 3 independent technical replicates for each of the four experimental conditions (12 arrays total) and assessed by a non-parametric rank product test. The statistical significance of differential gene expression and false discovery rates (FDR) were derived through a Rank Product algorithm available as an analysis package in R (http://www.r-product.org/).

source_name: bone marrow Gr1CD11b cells from heterozygous Leiden mice with hematopoietic EPCR-deficiency infused with EPCR-depleted bone marrow cells-7 days after infection with S.aureus-rep 1

label_protocol: 100 ng RNA was amplified using an Affymetrix two-cycle cDNA synthesis kit.   cRNA was synthesized, labeled with biotin, and fragmented in accordance with the Affymetrix GeneChip expression analysis technical manual.

extract_protocol: Trizol extraction of total RNA was performed according to the manufacturer's instructions

tag: background strain

value: heterozygous Leiden


title: BAM8-Lig_Ox, untreated, biological rep1

description:
BAM8-Lig_Ox_A
Gene expression data from 3-week-old shoots; untreated plants.

source_name: shoots, BAM8-Lig_Ox, untreated


title: BAM7-Lig_Ox, untreated, biological rep1

description:
BAM7-Lig_Ox_A
Gene expression data from 3-week-old shoots; untreated plants.

source_name: shoots, BAM7-Lig_Ox, untreated


title: BAM7-Lig_Ox, untreated, biological rep2

description:
BAM7-Lig_Ox_B
Gene expression data from 3-week-old shoots; untreated plants.


title: BAM8-Cat_Ox, untreated, biological rep1

description:
BAM8-Cat_Ox_A
Gene expression data from 3-week-old shoots; untreated plants.

source_name: shoots, BAM8-Cat_Ox, untreated


title: adoptive transfer wt BM-1

description: heterozygous Leiden carriers with hematopoietic EPCR deficiency  were adoptively transferred with 10exp6 CD11c-POS splenocytes plus 10exp6 wildtype bone marrow cells. One day later, mice were infected intraperitoneally with S.aureus. & days later,  (Gr1-CD11b)-POS bone marrow cells were isolated by FACS and total RNA was prepared for expression analysis

source_name: bone marrow Gr1CD11b cells from heterozygous Leiden mice with hematopoietic EPCR-deficiency infused with wildtype bone marrow cells-7 days after infection with S.aureus-rep 1


title: BAM7-Cat_Ox, untreated, biological rep1

description:
BAM7-Cat_Ox_A
Gene expression data from 3-week-old shoots; untreated plants.

source_name: shoots, BAM7-Cat_Ox, untreated


title: adoptive transfer depleted BM-1

description: heterozygous Leiden carriers with hematopoietic EPCR deficiency  were adoptively transferred with 10exp6 CD11c-POS splenocytes plus 10exp6 wildtype bone marrow cells depleted by FACS of (EPCR-F4/80)-POS cells. One day later, mice were infected intraperitoneally with S.aureus. & days later,  (Gr1-CD11b)-POS bone marrow cells were isolated by FACS and total RNA was prepared for expression analysis


title: BAM7-Cat_Ox, untreated, biological rep2

description:
BAM7-Cat_Ox_B
Gene expression data from 3-week-old shoots; untreated plants.


title: fV QQ LPS-aPC

description: homozygous Leiden mice, CD11cPOS splenocytes, 16h post intraperitoneal challenge with 40 mg/kg LPS, followed within 15 min by intravenous infusion of 10 μg recombinant mouse 5A-aPC

source_name: spleen dendritic cells-homozygous Leiden mice-16 hours after LPS/aPC

value: homozygous Leiden


title: RAW LPS normal plasma

description: RAW264.7 cells treated for 3h with LPS in the presence of 10% v/v plasma from wildtype mice

source_name: RAW264.7 cell-normal plasma-3 hours after LPS

value: Balb/c

value: wildtype


title: RAW LPS-aPC fV QQ plasma

description: RAW264.7 cells treated for 3h with LPS and recombinant mouse aPC (100 ng/mL each) in the presence of 10% v/v plasma prepared from homozygous Leiden mice

source_name: RAW264.7 cell-homozygous Leiden plasma-3 hours after LPS/aPC


title: wildtype LPS-aPC

description: wildtype mice, CD11cPOS splenocytes, 16h post intraperitoneal challenge with 40 mg/kg LPS, followed within 15 min by intravenous infusion of 10 μg recombinant mouse 5A-aPC

source_name: spleen dendritic cells-wildtype mice-16 hours after LPS/aPC


title: RAW LPS-aPC normal plasma

description: RAW264.7 cells treated for 3h with LPS and recombinant mouse aPC (100 ng/mL each) in the presence of 10% v/v plasma prepared from wildtype mice

source_name: RAW264.7 cell-normal plasma-3 hours after LPS/aPC


title: adoptive transfer wt BM-2

source_name: bone marrow Gr1CD11b cells from heterozygous Leiden mice with hematopoietic EPCR-deficiency infused with wildtype bone marrow cells-7 days after infection with S.aureus-rep 2


title: fV QQ LPS

description: homozygous Leiden mice, 16h post intraperitoneal challenge with 40 mg/kg LPS, CD11cPOS splenocytes

source_name: spleen dendritic cells-homozygous Leiden mice-16 hours after LPS


title: wildtype LPS

description: wildtype mice, 16h post intraperitoneal challenge with 40 mg/kg LPS, CD11c-POS splenocytes

source_name: spleen dendritic cells-wildtype mice-16 hours after LPS


title: BAM8-Lig_Ox, untreated, biological rep2

description:
BAM8-Lig_Ox_B
Gene expression data from 3-week-old shoots; untreated plants.