title: SScMH_28_base_LA

status: Public on Jul 25 2014

city: Lebanon

first: Tammara

last: Wood

state: NH

address: WTRB 674

department: Biomedical Data Science

institute: Dartmouth Medical School

description:
SScMH_28_base_LA
EXPT_ID: 72495
PRINT_ID: 1064

hyb_protocol: Hybridized using Agilent Hyb Kit.

scan_protocol: Fluorescent array images were collected for both Cy3 and Cy5 with a GenePix 4000B fluorescent scanner, and image intensity data were extracted and analyzed with GenePix Pro analysis software.

data_processing: Data for both channels were Lowess-normalized and then the log(2) ratio was taken. Data were filtered for genes with >80% good data.

label_protocol: Labeled using Agilent Quick Amp Labeling Kit.

extract_protocol: Total RNA isolated using Qiagen RNeasy Mini Kit.

treatment_protocol: Storage of human tissue in RNAlater.

tag: tissue

value: systemic sclerosis human forearm skin biopsy

source_name: Stratagene Universal Human Reference

extract_protocol: Total RNA isolated by Stratagene.

tag: sample type

value: reference


title: PSI-less/Scp-less, Biological replicate 3 of 3

status: Public on Mar 30 2015

type: RNA

city: Sydney

first: Miguel

middle: A

last: Hernández-Prieto

state: NSW

address: Macleay Building A12

department: School of life and environmental sciences

country: Australia

institute: University of Sydney

hyb_protocol: 500 ng Cy3-labelled sample was hybridized overnight at 65 °C following instruction of Agilent's Gene Expression Hybridization kit. Washes was performed using wash buffers from Gene Expression Wash Pack (Agilent) and following the manufacturers instructions.

scan_protocol: Slides were scanned on the Agilent Technologies Scanner (G2565CA) using scan profile AgilentHD_GX_1Color (Agilent HD 1- color gene expression microarrays).

data_processing: The scanned images were analyzed with Agilent's Feature Extraction program version 10.5.1.1 (Protocol: GE1_105_Dec08). gProcessedSignal value and SystematicName were used to get normalized signal intensities. Data were normalized in R using the bioconductor package OLIN.

molecule: total RNA

organism: Synechocystis sp. PCC 6803

source_name: Photosystem I less strain grown under low light and 10 mM glucose

label_protocol: Cyanine-3 (Cy3) labeled cDNA was prepared from 7 μg of total RNA using One-Color Microarray-Based Prokaryote Analysis (FairPlay III Labeling) Protocol Version 1.3 (August 2010) (Agilent). Agilent’s RNA Spike-In Kit was added to the samples allowing to monitor microarray workflow. Dye incorporation and cDNA concentration were measured with the NanoDrop ND-2000 Spectrophotometer.

growth_protocol: Synechocystis 6803 PSI-less and PSI-less/ScpABCDE- mutants were cultivated at 30°C at a light intensity of 4–5 μmol photons m–2 s–1 in BG-11 growth medium (Rippka et al 1979) supplemented with 10 mM glucose.

extract_protocol: RNA isolation was done as described previously (Eisenhut, M., von Wobeser, E.A., Jonas, L., Schubert, H., Ibelings, B.W., Bauwe, H. et al. (2007) Plant Physiol. 144: 1946-1959).

tag: strain

value: Photosystem I-less

source_name: Photosystem I-less/ScpABCDE-less strain grown under low light and 10 mM glucose

value: Photosystem I-less/ScpABCDE-less


title: SScMH_30_6mo_LA

country: USA

description:
SScMH_30_6mo_LA
EXPT_ID: 72501
PRINT_ID: 1064

organism: Homo sapiens


title: SScMH_26_base_LF

description:
SScMH_26_base_LF
EXPT_ID: 72450
PRINT_ID: 1064

value: systemic sclerosis human back skin biopsy


title: SScMH_27_base_LF

description:
SScMH_27_base_LF
EXPT_ID: 72484
PRINT_ID: 1064


title: SScReg_1207_LA

description:
SScReg_1207_LA
EXPT_ID: 72335
PRINT_ID: 1064


title: SScMH_30_6mo_LF

description:
SScMH_30_6mo_LF
EXPT_ID: 72502
PRINT_ID: 1064


title: SScMH_30_base_LF

description:
SScMH_30_base_LF
EXPT_ID: 72504
PRINT_ID: 1064


title: SScMH_32_base_RA

description:
SScMH_32_base_RA
EXPT_ID: 72499
PRINT_ID: 1064


title: SScMH_28_base_LF

description:
SScMH_28_base_LF
EXPT_ID: 72497
PRINT_ID: 1064


title: SScReg_1207_LF

description:
SScReg_1207_LF
EXPT_ID: 72336
PRINT_ID: 1064


title: SScMH_27_6mo_LF

description:
SScMH_27_6mo_LF
EXPT_ID: 72483
PRINT_ID: 1064


title: SScMH_32_6mo_RA

description:
SScMH_32_6mo_RA
EXPT_ID: 72498
PRINT_ID: 1064


title: SScMH_27_base_LF_replicate

description:
SScMH_27_base_LF
EXPT_ID: 72488
PRINT_ID: 1064


title: SScMH_28_6mo_LF

description:
SScMH_28_6mo_LF
EXPT_ID: 72494
PRINT_ID: 1064


title: SScMH_26_base_LA

description:
SScMH_26_base_LA
EXPT_ID: 72445
PRINT_ID: 1064


title: SScMH_27_6mo_LA

description:
SScMH_27_6mo_LA
EXPT_ID: 72487
PRINT_ID: 1064


title: SScMH_32_base_RF

description:
SScMH_32_base_RF
EXPT_ID: 72500
PRINT_ID: 1064


title: SScMH_28_6mo_LA

description:
SScMH_28_6mo_LA
EXPT_ID: 72493
PRINT_ID: 1064


title: SScMH_30_base_LA

description:
SScMH_30_base_LA
EXPT_ID: 72503
PRINT_ID: 1064


title: SScMH_26_6mo_LF

description:
SScMH_26_6mo_LF
EXPT_ID: 72446
PRINT_ID: 1064


title: SScMH_32_6mo_RF

description:
SScMH_32_6mo_RF
EXPT_ID: 72496
PRINT_ID: 1064


title: SScMH_26_6mo_LA

description:
SScMH_26_6mo_LA
EXPT_ID: 72448
PRINT_ID: 1064


title: SScMH_25_base_LF

description:
SScMH_25_base_LF
EXPT_ID: 72444
PRINT_ID: 1064


title: SScMH_25_base_LA

description:
SScMH_25_base_LA
EXPT_ID: 72449
PRINT_ID: 1064


title: SScMH_25_6mo_LA

description:
SScMH_25_6mo_LA
EXPT_ID: 72443
PRINT_ID: 1064


title: SScMH_25_6mo_LF

description:
SScMH_25_6mo_LF
EXPT_ID: 72447
PRINT_ID: 1064


title: SScMH_25_12mo_LA

description:
SScMH_25_12mo_LA
EXPT_ID: 72482
PRINT_ID: 1064


title: SScMH_23_Base_LF

description:
SScMH_23_Base_LF
EXPT_ID: 72332
PRINT_ID: 1064


title: SScMH_23_Base_LA

description:
SScMH_23_Base_LA
EXPT_ID: 72328
PRINT_ID: 1064


title: Mouse_Muscle_Dysf w ACVR2B/Fc_1

status: Public on Sep 04 2015

city: Baltimore

first: Yun-Sil

last: Lee

state: Maryland

address: 725 North Wolfe Street, PCTB 803

department: Molecular Biology and Genetics

institute: Johns Hopkins University School of Medicine

hyb_protocol: 17hrs at 45° C with rotation (60rpm) as described by Affymetrix in their GeneChip Expression Analysis Technical Manual

scan_protocol: Using Affymetrix’ GeneChip Scanner 3000 7G and default parameters described by the manufacturer in their GeneChip Expression Analysis Technical Manual

data_processing:
Affymetrix CEL files were extracted and their data normalized with the Partek GS 6.6 platform.  Partek's Extended Meta-Probeset set was used with RMA normalization to create quantile-normalized log2 transcript signal values, which were used in subsequent ANOVA analyses.
MoEx-1_0-st-v1.r2.pgf
MoEx-1_0-st-v1.na32.mm9.probeset_extended.mps

label: biotin

organism: Mus musculus

source_name: 10 weeks_quadriceps muscle

label_protocol: The Ambion Expression WT kit was used as described in the manufacturer's manual.

growth_protocol: None, surgical biopsy

extract_protocol: Quadriceps muscle (100 mg) was homogenized in TRIzol Reagent (Life Technologies), and RNA was isolated from the supernatant with the RNeasy Mini Kit (Qiagen), according to the manufacturer’s instructions. All samples were treated with RNase-free DNase set (Qiagen) to remove trace amounts of genomic DNA.

treatment_protocol: Male C57BL/6 (wt) and Dysf-/- mice beginning at 6 weeks of age were given four weekly Intraperitoneal injections of ACVR2B/Fc (10 mg kg-1, i.e., 200 ug per injection)

value: quadriceps muscle

tag: treatment

tag: genotype/variation

value: Dysf-/-

tag: gender

value: male


title: SScMH_25_12mo_LF

description:
SScMH_25_12mo_LF
EXPT_ID: 72486
PRINT_ID: 1064


title: SScMH_23_6mo_LA

description:
SScMH_23_6mo_LA
EXPT_ID: 72327
PRINT_ID: 1064


title: SScMH_23_12mo_LF

description:
SScMH_23_12mo_LF
EXPT_ID: 72490
PRINT_ID: 1064


title: SScMH_21_6mo_LA

description:
SScMH_21_6mo_LA
EXPT_ID: 70417
PRINT_ID: 1064


title: SScMH_23_6mo_LF

description:
SScMH_23_6mo_LF
EXPT_ID: 72331
PRINT_ID: 1064


title: SScMH_21_6mo_LF

description:
SScMH_21_6mo_LF
EXPT_ID: 70418
PRINT_ID: 1064


title: SScMH_20_6mo_LF

description:
SScMH_20_6mo_LF
EXPT_ID: 70415
PRINT_ID: 1064


title: SScMH_21_base_LA

description:
SScMH_21_base_LA
EXPT_ID: 70395
PRINT_ID: 1064


title: SScMH_23_12mo_LA

description:
SScMH_23_12mo_LA
EXPT_ID: 72489
PRINT_ID: 1064


title: SScMH_19_6mo_LF

description:
SScMH_19_6mo_LF
EXPT_ID: 70412
PRINT_ID: 1064


title: SScMH_20_base_LA

description:
SScMH_20_base_LA
EXPT_ID: 70394
PRINT_ID: 1064


title: SScMH_19_6mo_LA

description:
SScMH_19_6mo_LA
EXPT_ID: 70411
PRINT_ID: 1064


title: SScMH_19_12mo_LF

description:
SScMH_19_12mo_LF
EXPT_ID: 72343
PRINT_ID: 1064


title: SScMH_20_base_LF

description:
SScMH_20_base_LF
EXPT_ID: 70416
PRINT_ID: 1064


title: SScMH_21_12mo_LF

description:
SScMH_21_12mo_LF
EXPT_ID: 72485
PRINT_ID: 1064


title: SScMH_20_6mo_LA

description:
SScMH_20_6mo_LA
EXPT_ID: 70414
PRINT_ID: 1064


title: SScMH_17_Base_LF

description:
SScMH_17_Base_LF
EXPT_ID: 72326
PRINT_ID: 1064


title: SScMH_17_12mo_LA

description:
SScMH_17_12mo_LA
EXPT_ID: 72325
PRINT_ID: 1064


title: SScMH_19_12mo_LA

description:
SScMH_19_12mo_LA
EXPT_ID: 72339
PRINT_ID: 1064


title: SScMH_17_Base_LA

description:
SScMH_17_Base_LA
EXPT_ID: 72330
PRINT_ID: 1064


title: SScMH_19_base_LF

description:
SScMH_19_base_LF
EXPT_ID: 70413
PRINT_ID: 1064


title: SScMH_17_6mo_LA

description:
SScMH_17_6mo_LA
EXPT_ID: 72329
PRINT_ID: 1064


title: SScMH_21_base_LF

description:
SScMH_21_base_LF
EXPT_ID: 70419
PRINT_ID: 1064


title: SScMH_16_12mo_LF

description:
SScMH_16_12mo_LF
EXPT_ID: 72480
PRINT_ID: 1064


title: SScMH_21_12mo_LA

description:
SScMH_21_12mo_LA
EXPT_ID: 72481
PRINT_ID: 1064


title: SScMH_16_12mo_LA

description:
SScMH_16_12mo_LA
EXPT_ID: 72479
PRINT_ID: 1064


title: SScMH_19_base_LA

description:
SScMH_19_base_LA
EXPT_ID: 70393
PRINT_ID: 1064


title: SScMH_15_base_LA

description:
SScMH_15_base_LA
EXPT_ID: 70391
PRINT_ID: 1064


title: SScMH_15_6mo_LF

description:
SScMH_15_6mo_LF
EXPT_ID: 72478
PRINT_ID: 1064


title: SScMH_15_base_LF

description:
SScMH_15_base_LF
EXPT_ID: 70410
PRINT_ID: 1064


title: SScMH_15_6mo_LA

description:
SScMH_15_6mo_LA
EXPT_ID: 70409
PRINT_ID: 1064


title: SScMH_12_12mo_LA

description:
SScMH_12_12mo_LA
EXPT_ID: 72477
PRINT_ID: 1064


title: SScMH_15_12mo_LA

description:
SScMH_15_12mo_LA
EXPT_ID: 72338
PRINT_ID: 1064


title: SScMH_14_12mo_LA

description:
SScMH_14_12mo_LA
EXPT_ID: 72431
PRINT_ID: 1064


title: SScMH_15_12mo_LF

description:
SScMH_15_12mo_LF
EXPT_ID: 72342
PRINT_ID: 1064


title: SScMH_12_12mo_RF

description:
SScMH_12_12mo_RF
EXPT_ID: 72430
PRINT_ID: 1064


title: SScMH_07_24mo_LA

description:
SScMH_07_24mo_LA
EXPT_ID: 72340
PRINT_ID: 1064


title: SScMH_08_24mo_LF

description:
SScMH_08_24mo_LF
EXPT_ID: 72492
PRINT_ID: 1064


title: SScMH_08_24mo_LA

description:
SScMH_08_24mo_LA
EXPT_ID: 72491
PRINT_ID: 1064


title: SScMH_07_24mo_LF

description:
SScMH_07_24mo_LF
EXPT_ID: 72344
PRINT_ID: 1064


title: SScMH_06_24mo_RF

description:
SScMH_06_24mo_RF
EXPT_ID: 72432
PRINT_ID: 1064


title: SScMH_03_24mo_LA

description:
SScMH_03_24mo_LA
EXPT_ID: 70405
PRINT_ID: 1064


title: SScMH_03_24mo_LF

description:
SScMH_03_24mo_LF
EXPT_ID: 70406
PRINT_ID: 1064


title: SScMH_10_24mo_LA

description:
SScMH_10_24mo_LA
EXPT_ID: 72337
PRINT_ID: 1064


title: SScMH_06_24mo_RA

description:
SScMH_06_24mo_RA
EXPT_ID: 72435
PRINT_ID: 1064


title: SScMH_10_24mo_LF

description:
SScMH_10_24mo_LF
EXPT_ID: 72341
PRINT_ID: 1064


title: MH_Norm_10_LF

description:
MH_Norm_10_LF
EXPT_ID: 72334
PRINT_ID: 1064

extract_protocol: Total RNA isolated using Qiagen Fibrous Tissue Mini Kit.

value: normal human back skin biopsy


title: SScMH_08_12mo_LF

description:
SScMH_08_12mo_LF
EXPT_ID: 72436
PRINT_ID: 1064


title: SScMH_04_24mo_LF

description:
SScMH_04_24mo_LF
EXPT_ID: 70408
PRINT_ID: 1064


title: MH_Norm_10_LA

description:
MH_Norm_10_LA
EXPT_ID: 72333
PRINT_ID: 1064

value: normal human forearm skin biopsy


title: SScMH_08_12mo_LA

description:
SScMH_08_12mo_LA
EXPT_ID: 72433
PRINT_ID: 1064


title: Liver expressing TGFα and c-Myc transgene, then turned off for 12weeks Replicate 1

status: Public on Jul 15 2014

city: Madison

first: Ly

middle: L

last: Pham

state: WI

address: 2015 Linden Drive

department: Pathobiological Sciences

institute: UW-Madison

description: Biological Replicate 1 of 3. Liver expression TGFα and c-Myc transgene, Transgene turn off by Dox administration for 12 weeks

hyb_protocol: Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential

scan_protocol: Scanned on an Agilent Technologies Scanner G2505C US10130359.  Images were quantified using Agilent Feature Extraction Software.

data_processing: Limma was used for LOWESS normalization.

source_name: Mouse Liver with TGFα and c-Myc transgene

label_protocol: 1.2 ug of RNA which was primed with T7 promoter primer from Agilent's two-color Low RNA Input Linear Amplification kit. Then, 1 ul of 10mM dNTPs, 2ul 0.1M DTT and MMLV RT. That goes 2.5 hours at 40C, then 15 min at 65C. Then transcribed with 3ul DTT, 4 ul NTP mix, 3.2 ul 50% PEG, T7 polymerase, and 1.2 ul cy 3 or 5-CTP per sample for 2.5 hours at 40C

extract_protocol: Total RNA extracted using Qiagen protocol following manufacturer's instructions

tag: weeks on dox

tag: transgene

value: TGFα and c-Myc

source_name: Pooled mixed-gender reference mice

value: Liver


title: Liver expressing TGFα and c-Myc transgene, then turned off for 12weeks Replicate 3

description: Biological Replicate 3 of 3. Liver expression TGFα and c-Myc transgene, Transgene turn off by Dox administration for 12 weeks


title: SScMH_04_24mo_LA

description:
SScMH_04_24mo_LA
EXPT_ID: 70407
PRINT_ID: 1064


title: Liver expressing TGFα and c-Myc transgene, then turned off for 4weeks Replicate 2

description: Biological Replicate 2 of 3. Liver expression TGFα and c-Myc transgene, Transgene turn off by Dox administration for 4 weeks


title: SScMH_14_12mo_LF

description:
SScMH_14_12mo_LF
EXPT_ID: 72434
PRINT_ID: 1064


title: Liver expressing TGFα and c-Myc transgene, then turned off for 4weeks Replicate 3

description: Biological Replicate 3 of 3. Liver expression TGFα and c-Myc transgene, Transgene turn off by Dox administration for 4 weeks


title: Liver expressing TGFα and c-Myc transgene, then turned off for 12weeks Replicate 2

description: Biological Replicate 2 of 3. Liver expression TGFα and c-Myc transgene, Transgene turn off by Dox administration for 12 weeks


title: Liver expressing TGFα and c-Myc transgene, then turned off for 4weeks Replicate 1

description: Biological Replicate 1 of 3. Liver expression TGFα and c-Myc transgene, Transgene turn off by Dox administration for 4 weeks


title: Liver expressing TGFα  transgene, then turned off for 12weeks Replicate 3

description: Biological Replicate 3 of 3. Liver expression TGFα transgene, Transgene turn off by Dox administration for 12 weeks

source_name: Mouse Liver with TGFα transgene

value: TGFα


title: Liver tumor expressing TGFα and c-Myc transgene Replicate 2

description: Biological Replicate 2 of 3. Liver tumor expression TGFα and c-Myc transgene

source_name: Individual Mouse Liver Tumor with c-Myc and TGFα transgene


title: Liver tumor expressing TGFα and c-Myc transgene Replicate 3

description: Biological Replicate 3 of 3. Liver tumor expression TGFα and c-Myc transgene


title: Liver expressing TGFα  transgene, then turned off for 4weeks Replicate 3

description: Biological Replicate 3 of 3. Liver expression TGFα transgene, Transgene turn off by Dox administration for 4 weeks


title: Liver expressing TGFα  transgene, then turned off for 12weeks Replicate 1

description: Biological Replicate 1 of 3. Liver expression TGFα transgene, Transgene turn off by Dox administration for 12 weeks


title: Liver expressing TGFα  transgene, then turned off for 4weeks Replicate 2

description: Biological Replicate 2 of 3. Liver expression TGFα transgene, Transgene turn off by Dox administration for 4 weeks


title: Liver tumor expressing TGFα transgene Replicate 3

description: Biological Replicate 3 of 3. Liver tumor expression TGFα transgene

source_name: Individual Mouse Liver Tumor with TGFα transgene


title: Liver tumor expressing TGFα and c-Myc transgene Replicate 1

description: Biological Replicate 1 of 3. Liver tumor expression TGFα and c-Myc transgene


title: Liver tumor expressing TGFα transgene Replicate 1

description: Biological Replicate 1 of 3. Liver tumor expression TGFα transgene


title: Liver expressing TGFα  transgene, then turned off for 4weeks Replicate 1

description: Biological Replicate 1 of 3. Liver expression TGFα transgene, Transgene turn off by Dox administration for 4 weeks


title: Liver expressing TGFα  transgene, then turned off for 12weeks Replicate 2

description: Biological Replicate 2 of 3. Liver expression TGFα transgene, Transgene turn off by Dox administration for 12 weeks


title: Liver expressing Myc transgene, then turned off for 4weeks Replicate 3

description: Biological Replicate 3 of 3. Liver expression c-Myc transgene, Transgene turn off by Dox administration for 4 weeks

source_name: Mouse Liver with c-Myc transgene

value: c-Myc


title: Liver tumor expressing Myc transgene Replicate 7

description: Biological Replicate 7 of 7. Liver tumor expression c-Myc transgene

source_name: Individual Mouse Liver Tumor with c-Myc transgene


title: Liver tumor expressing TGFα transgene Replicate 2

description: Biological Replicate 2 of 3. Liver tumor expression TGFα transgene


title: Liver tumor expressing Myc transgene Replicate 5

description: Biological Replicate 5 of 7. Liver tumor expression c-Myc transgene


title: Liver expressing Myc transgene, then turned off for 12weeks Replicate 3

description: Biological Replicate 3 of 3. Liver expression c-Myc transgene, Transgene turn off by Dox administration for 12 weeks


title: Liver tumor expressing Myc transgene Replicate 6

description: Biological Replicate 6 of 7. Liver tumor expression c-Myc transgene


title: Liver expressing Myc transgene, then turned off for 4weeks Replicate 2

description: Biological Replicate 2 of 3. Liver expression c-Myc transgene, Transgene turn off by Dox administration for 4 weeks


title: Liver tumor expressing Myc transgene Replicate 4

description: Biological Replicate 4 of 7. Liver tumor expression c-Myc transgene


title: Control liver Replicate 1

description: Biological Replicate 1 of 3. Control Normal Mouse Liver

source_name: Mouse Liver, Control


title: Liver tumor expressing Myc transgene Replicate 1

description: Biological Replicate 1 of 7. Liver tumor expression c-Myc transgene


title: Liver expressing Myc transgene, then turned off for 12weeks Replicate 1

description: Biological Replicate 1 of 3. Liver expression c-Myc transgene, Transgene turn off by Dox administration for 12 weeks


title: Liver tumor expressing Myc transgene Replicate 2

description: Biological Replicate 2 of 7. Liver tumor expression c-Myc transgene


title: Liver expressing Myc transgene, then turned off for 12weeks Replicate 2

description: Biological Replicate 2 of 3. Liver expression c-Myc transgene, Transgene turn off by Dox administration for 12 weeks


title: Liver tumor expressing Myc transgene Replicate 3

description: Biological Replicate 3 of 7. Liver tumor expression c-Myc transgene


title: Control liver Replicate 2

description: Biological Replicate 2 of 3. Control Normal Mouse Liver


title: Control liver Replicate 3

description: Biological Replicate 3 of 3. Control Normal Mouse Liver


title: Liver expressing Myc transgene, then turned off for 4weeks Replicate 1

description: Biological Replicate 1 of 3. Liver expression c-Myc transgene, Transgene turn off by Dox administration for 4 weeks


title: cultured fibroblast_INTS8 mutation III.2

status: Public on Sep 01 2013

city: Rotterdam

first: Renske

last: Oegema

address: Postbus 2040

department: Clinical Genetics

country: Netherlands

institute: Erasmus MC

description:
cultured fibroblast_INTS8 mutation
individual III.2

hyb_protocol: according to Affymetrix protocol

scan_protocol: according to Affymetrix protocol

data_processing:
Six Affymetrix Gene Chip 1.0 ST exon arrays were rma normalized on transcript level using the R package oligo (http://www.r-project.org).
probe group file: HuEx-1_0-st-v2.r2.pgf
meta-probeset file: HuEx-1_0-st-v2.na30.hg19.transcript.csv
logFC are contrasts of linear fit models; p.values based on empirical Bayes shrinkage (see linked as supplementary file on Series record)

source_name: cultured fibroblast_INTS8 mutation

label_protocol: according to Affymetrix protocol

growth_protocol: fibroblasts cultured in DMEM/FCS/PS, 10% CO2

extract_protocol: Invitrogen: Trizol LS followed by Qiagen Rneasy Mini Kit

treatment_protocol: not applicable

tag: individual

tag: cell type

value: cultured fibroblast

value: INTS8 mutation


title: cultured fibroblast_control C2

description:
cultured fibroblast_control
C2

source_name: cultured fibroblast_control

value: control


title: cultured fibroblast_control C3

description:
cultured fibroblast_control
C3


title: cultured fibroblast_INTS8 mutation III.3


title: cultured fibroblast_INTS8 mutation III.4

description:
cultured fibroblast_INTS8 mutation
individual III.4


title: cultured fibroblast_control C1

description:
cultured fibroblast_control
C1


title: B-cell precursors at S3_FBM14_S3_DNA methylation

status: Public on Mar 26 2013

type: genomic

city: Seoul

first: Seung-Tae

state: - None -

address: 50 Iron-Dong KanangmGu

department: Laboratory Medicine & Genetics

country: South Korea

institute: Samsung Medical Center

description: Methylation data of early B-cell precursors from human fetuses ranging in age between 20 and 24 weeks of gestation

hyb_protocol: bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol

scan_protocol: Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting

data_processing:
Illumina GenomeStudio software
matrix signal intensities value definition:Unmethylated and methylated signal intensities

label: Cy5 and Cy3

molecule: genomic DNA

source_name: B-cell precursors at S3

label_protocol: Standard Illumina Protocol

extract_protocol: AllPrep DNA/RNA Mini Kit (Qiagen) according to manufacturer's instructions

value: B-cell precursors at S3, predominantly pre-B-II cells

value: fetal bone marrow

tag: subjects

value: fetuses at San Francisco area

tag: age

value: normal fetuses ranging in age between 20 and 24 weeks of gestation


title: B-cell precursors at S4_FBM29_S4_DNA methylation

source_name: B-cell precursors at S4

value: B-cell precursors at S4, predominantly immature B cells


title: B-cell precursors at S4_FBM17_S4_DNA methylation


title: B-cell precursors at S3_FBM30_S3_DNA methylation


title: B-cell precursors at S2_FBM19_S2_DNA methylation

source_name: B-cell precursors at S2

value: B-cell precursors at S2, predominantly pre-B-I cells


title: B-cell precursors at S3_FBM18_S3_DNA methylation


title: B-cell precursors at S1_FBM12_S1_DNA methylation

source_name: B-cell precursors at S1

value: B-cell precursors at S1, predominantly multipotent progenitors and common lymphoid progenitors


title: B-cell precursors at S4_FBM9_S4_DNA methylation


title: B-cell precursors at S3_FBM10_S3_DNA methylation


title: B-cell precursors at S3_FBM26_S3_DNA methylation


title: B-cell precursors at S1_FBM32_S1_DNA methylation


title: Squamous_cell_carcinoma_sample_39A

status: Public on Nov 01 2011

city: Uppsala

first: Anders

last: Isaksson

address: Akademiska sjukhuset ing61 3tr

country: Sweden

institute: Uppsala University

description:
PA026_human_39A_070522
Hybridized to 250K_Nsp

hyb_protocol: Array experiments were performed according to the standard protocols for Affymetrix GeneChip® Mapping 250K Nsp I arrays (Gene Chip Mapping 500K Assay Manual, P/N 701930 Rev2., Affymetrix Inc., Santa Clara, CA, U.S.A. ). The arrays were then washed using Affymetrix fluidics stations.

scan_protocol: The arrays were scanned using the GeneChip® Scanner 3000 7G.

data_processing: Copy number normalization, to produce log(2) ratios, was performed using the Copy Number Analysis Tool (CNAT) 4.0.1. The reference set was 82 normal samples of Caucasian origin, analyzed at the Uppsala Array Platform. Subsequent copy number analyses and group comparisons were performed using the software BioDiscovery Nexus Copy Number 3.0 and the built-in Rank Segmentation algorithm, for estimating copy numbers and segmenting each interval.

label: Biotin

source_name: Non-small cell lung cancer tissue, long-term survival, squamous cell carcinoma

extract_protocol: DNA was extracted from 2-10 frozen tissue sections (10mm) using the QIAamp DNA Mini Kit (Qiagen, Hilden, Germany) and the RNeasy Mini Kit (Qiagen), respectively.

value: non-small cell lung cancer

tag: histology

value: squamous cell carcinoma

tag: survival

value: long

tag: registry year


title: B-cell precursors at S1_FBM16_S1_DNA methylation


title: Adenocarcinoma_sample_37A

description:
PA026_human_37A_070314
Hybridized to 250K_Nsp

source_name: Non-small cell lung cancer tissue, long-term survival, adenocarcinoma

label_protocol: Array experiments were performed according to the standard protocols for Affymetrix GeneChip® Mapping 250K Nsp I arrays (Gene Chip Mapping 500K Assay Manual, P/N 701930 Rev2., Affymetrix Inc., Santa Clara, CA, U.S.A. ).

value: adenocarcinoma


title: B-cell precursors at S1_FBM28_S1_DNA methylation


title: Squamous_cell_carcinoma_sample_6A

description:
PA026_human_6A_070314
Hybridized to 250K_Nsp


title: B-cell precursors at S2_FBM27_S2_DNA methylation


title: B-cell precursors at S1_FBM20_S1_DNA methylation


title: B-cell precursors at S2_FBM23_S2_DNA methylation


title: Adenocarcinoma_sample_81A

description:
PA026_human_81A_070511
Hybridized to 250K_Nsp

source_name: Non-small cell lung cancer tissue, short-term survival, adenocarcinoma

value: short


title: Squamous_cell_carcinoma_sample_88A

description:
PA026_human_88A_070403
Hybridized to 250K_Nsp

source_name: Non-small cell lung cancer tissue, short-term survival, squamous cell carcinoma


title: Adenocarcinoma_sample_373B

description:
PA026_human_373B_070918
Hybridized to 250K_Nsp


title: B-cell precursors at S2_FBM11_S2_DNA methylation


title: Large_cell_carcinoma_sample_87A

description:
PA026_human_87A_070403
Hybridized to 250K_Nsp

source_name: Non-small cell lung cancer tissue, short-term survival, large cell carcinoma

value: large cell carcinoma


title: B-cell precursors at S3_FBM22_S3_DNA methylation


title: B-cell precursors at S1_FBM24_S1_DNA methylation


title: B-cell precursors at S2_FBM31_S2_DNA methylation


title: B-cell precursors at S4_FBM21_S4_DNA methylation


title: Adenocarcinoma_sample_48A

description:
PA026_human_48A_070511
Hybridized to 250K_Nsp


title: B-cell precursors at S2_FBM15_S2_DNA methylation


title: Squamous_cell_carcinoma_sample_367A

description:
PA026_human_367A_070918
Hybridized to 250K_Nsp


title: Squamous_cell_carcinoma_sample_370A

description:
PA026_human_370A_070906
Hybridized to 250K_Nsp


title: Squamous_cell_carcinoma_sample_365A

description:
PA026_human_365A_070918
Hybridized to 250K_Nsp


title: Large_cell_carcinoma_sample_361A

description:
PA026_human_361A_070906
Hybridized to 250K_Nsp

source_name: Non-small cell lung cancer tissue, long-term survival, large cell carcinoma


title: Adenocarcinoma_sample_317A

description:
PA026_human_317A_071004
Hybridized to 250K_Nsp


title: Squamous_cell_carcinoma_sample_319A

description:
PA026_human_319A_070927
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_284A

description:
PA026_human_284A_070927
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_359A

description:
PA026_human_359A_070906
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_347A

description:
PA026_human_347A_070906
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_32A

description:
PA026_human_32A_070522
Hybridized to 250K_Nsp


title: Squamous_cell_carcinoma_sample_297A

description:
PA026_human_297A_070925
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_35A

description:
PA026_human_35A_070928
Hybridized to 250K_Nsp


title: Large_cell_carcinoma_sample_331A

description:
PA026_human_331A_070905
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_299B

description:
PA026_human_299B_070918
Hybridized to 250K_Nsp


title: Squamous_cell_carcinoma_sample_342A

description:
PA026_human_342A_070905
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_310A

description:
PA026_human_310A_070828
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_288A

description:
PA026_human_288A_070920
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_338A

description:
PA026_human_338A_070828
Hybridized to 250K_Nsp


title: Large_cell_carcinoma_sample_309A

description:
PA026_human_309A_070918
Hybridized to 250K_Nsp


title: Squamous_cell_carcinoma_sample_296A

description:
PA026_human_296A_070928
Hybridized to 250K_Nsp


title: Large_cell_carcinoma_sample_314A

description:
PA026_human_314A_071004
Hybridized to 250K_Nsp


title: Squamous_cell_carcinoma_sample_305A

description:
PA026_human_305A_070918
Hybridized to 250K_Nsp


title: Large_cell_carcinoma_sample_295B

description:
PA026_human_295B_070928
Hybridized to 250K_Nsp


title: Squamous_cell_carcinoma_sample_300A

description:
PA026_human_300A_070828
Hybridized to 250K_Nsp


title: Squamous_cell_carcinoma_sample_256A

description:
PA026_human_256A_070920
Hybridized to 250K_Nsp


title: Squamous_cell_carcinoma_sample_27A

description:
PA026_human_27A_070511
Hybridized to 250K_Nsp


title: Squamous_cell_carcinoma_sample_271A

description:
PA026_human_271A_070918
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_255A

description:
PA026_human_255A_070920
Hybridized to 250K_Nsp


title: Large_cell_carcinoma_sample_265A

description:
PA026_human_265A_070920
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_258A

description:
PA026_human_258A_071004
Hybridized to 250K_Nsp


title: Large_cell_carcinoma_sample_207A

description:
PA026_human_207A_070920
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_247A

description:
PA026_human_247A_070918
Hybridized to 250K_Nsp


title: Large_cell_carcinoma_sample_234A

description:
PA026_human_234A_070928
Hybridized to 250K_Nsp


title: Squamous_cell_carcinoma_sample_278A

description:
PA026_human_278A_070927
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_235A

description:
PA026_human_235A_070928
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_229A

description:
PA026_human_229A_070927
Hybridized to 250K_Nsp


title: Large_cell_carcinoma_sample_196A

description:
PA026_human_196A_070404
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_169D

description:
PA026_human_169D_070404
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_189A

description:
PA026_human_189A_070523
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_12B

description:
PA026_human_12B_070928
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_172C

description:
PA026_human_172C_070928
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_369A

description:
PA026_369A_081002_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: Squamous_cell_carcinoma_sample_14A

description:
PA026_human_14A_070314
Hybridized to 250K_Nsp


title: Squamous_cell_carcinoma_sample_13A

description:
PA026_human_13A_070522
Hybridized to 250K_Nsp


title: Squamous_cell_carcinoma_sample_98A

description:
PA026_98A_081001_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_61A

description:
PA026_61A_081001_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_375A

description:
PA026_375A_081002_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: Large_cell_carcinoma_sample_195B

description:
PA026_human_195B_070920
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_197B

description:
PA026_human_197B_070511
Hybridized to 250K_Nsp


title: Squamous_cell_carcinoma_sample_175B

description:
PA026_human_175B_070404
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_302A

description:
PA026_302A_081002_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: Squamous_cell_carcinoma_sample_344A

description:
PA026_344A_081002_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: Squamous_cell_carcinoma_sample_357A

description:
PA026_357A_081002_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: Squamous_cell_carcinoma_sample_274A

description:
PA026_274A_081002_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_311C

description:
PA026_311C_081002_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_11C

description:
PA026_human_11C_070516
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_292B

description:
PA026_292B_081002_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: Large_cell_carcinoma_sample_332Y

description:
PA026_332Y_081002_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_162A

description:
PA026_human_162A_070511
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_272B

description:
PA026_272B_081002_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: Squamous_cell_carcinoma_sample_291A

description:
PA026_291A_081002_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_341A

description:
PA026_341A_081002_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_301A

description:
PA026_301A_081002_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_282A

description:
PA026_282A_081002_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_112B

description:
PA026_human_112B_070928
Hybridized to 250K_Nsp


title: Large_cell_carcinoma_sample_260A

description:
PA026_260A_081002_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_228B

description:
PA026_228B_081002_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_165C

description:
PA026_human_165C_070404
Hybridized to 250K_Nsp


title: Squamous_cell_carcinoma_sample_249A

description:
PA026_249A_081002_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: Squamous_cell_carcinoma_sample_131A

description:
PA026_human_131A_070404
Hybridized to 250K_Nsp


title: Large_cell_carcinoma_sample_243A

description:
PA026_243A_081002_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_23A

description:
PA026_23A_081001_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_223A

description:
PA026_223A_081001_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: Huh-7 cells transfected with HCV replicon LucNS3-5.1 and treated with 25-hydroxycholesterol 24 hrs, 2

status: Public on Dec 25 2008

city: Ottawa

first: John

middle: Paul

last: Pezacki

state: ON

address: 150 Louis-Pasteur Pvt

department: Chemistry and Biomolecular Sciences

country: Canada

institute: University of Ottawa

hyb_protocol: standard Affymetrix procedures except for use of custom GNF chip washer

scan_protocol: standard Affymetrix procedures using GCS3000 scanner

data_processing: gcRMA, unlogged

label_protocol: 5 ug total RNA was used in a standard Affymetrix single amplification protocol.

extract_protocol: Total RNA was isolated from cell pellets using the RNeasy kit (Qiagen, Valencia, CA, USA).

treatment_protocol: RNA extraction from Huh-7 cells treated with compounds at the indicated time points was performed using RNeasy kits (Qiagen). The integrity and concentration of RNA was determined by microfluidic analysis on an Experion instrument (BioRad).


title: Adenocarcinoma_sample_224A

description:
PA026_224A_081002_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: Huh-7 cells transfected with HCV replicon NeoNS3-del5B (replication deficient), 1

treatment_protocol: RNA extraction from R7T1 beta-cells treated with compounds at the indicated time points was performed using RNeasy kits (Qiagen). The integrity and concentration of RNA was determined by microfluidic analysis on an Experion instrument (BioRad).


title: Huh-7 cells treated with 25-hydroxycholesterol -24 hr, 1

description: Huh-7 cells treated with 25-hydroxycholesterol-24 hr


title: Naïve Huh-7 cells, 2

description: Naïve Huh-7 cells

source_name: Naïve Huh-7 cells

tag: Naïve Huh-7 cells


title: Huh-7 cells transfected with HCV replicon NeoNS3-del5B (replication deficient), 2


title: Huh-7 cells transfected with HCV replicon LucNS3-5.1, 1


title: Huh-7 cells treated with 25-hydroxycholesterol - 24hr, 2


title: Huh-7 cells transfected with HCV replicon LucNS3-5.1 and treated with 25-hydroxycholesterol 24 hrs, 1


title: Huh-7 cells transfected with HCV replicon LucNS3-5.1, 2


title: Naïve Huh-7 cells, 1

description: Naïve Huh-7 cells


title: Large_cell_carcinoma_sample_203B

description:
PA026_203B_081001_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_215A

description:
PA026_215A_081002_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_171A

description:
PA026_171A_081001_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: 62SmokeH

status: Public on Oct 12 2007

city: San Francisco

first: Prescott

middle: Gurney

last: Woodruff

state: CA

address: 505 Parnassus Ave

department: Medicine

institute: UCSF

description: Total RNA from freshly isolated epithelial cells

hyb_protocol: standard Affymetrix protocol

scan_protocol: standard Affymetrix protocol

data_processing: RMA/Bioconductor

label: NuGen Ovation RNA amplification

source_name: airway epithelial brushing

label_protocol: standard Affymetrix protocol

extract_protocol: unknown

value: Healthy control


title: Adenocarcinoma_sample_22B

description:
PA026_22B_081001_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_192C

description:
PA026_192C_081001_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: 52SMOKES

value: Smoker


title: Large_cell_carcinoma_sample_158A

description:
PA026_158A_081001_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_213A

description:
PA026_213A_081001_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: 56SMOKES


title: 25SMOKEH


title: 46SMOKES


title: 15SMOKES


title: Adenocarcinoma_sample_201A

description:
PA026_201A_081001_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_193B

description:
PA026_193B_081001_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: Adenocarcinoma_sample_200A

description:
PA026_200A_081001_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: 47SMOKES


title: Large_cell_carcinoma_sample_212A

description:
PA026_212A_081001_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: 51SMOKES


title: Squamous_cell_carcinoma_sample_202A

description:
PA026_202A_081001_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: 48SMOKES


title: 33SMOKEH


title: 24SMOKEH


title: Large_cell_carcinoma_sample_174A

description:
PA026_174A_081001_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: Large_cell_carcinoma_sample_214A

description:
PA026_214A_081001_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: Large_cell_carcinoma_sample_133A

description:
PA026_133A_081001_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: 57SMOKEH


title: Squamous_cell_carcinoma_sample_217A

description:
PA026_217A_081001_(Mapping250K_Nsp)
Hybridized to 250K_Nsp


title: 44SMOKEH


title: 20SMOKEH


title: 54SMOKES


title: 26SMOKES


title: 12SMOKES


title: 31SMOKES


title: 64SmokeH


title: 38SMOKEH


title: 34SAGEA

value: Asthmatic at baseline


title: 17SMOKES


title: 18SMOKES


title: 22SMOKEH


title: 61SMOKEH


title: 22GOBMUC2H


title: 36SMOKEH


title: 08SAGEA


title: 50SMOKES


title: 22SAGEA


title: 34GOBMUC2H


title: 10SMOKES


title: 38GOBMUC2H


title: 39GOBMUC2A


title: 43SMOKEH


title: 17GOBMUC2H


title: 32GOBMUC2H


title: 28GOBMUC2A


title: 16GOBMUC2A


title: 21GOBMUC2H


title: 31GOBMUC2A


title: 13GOBMUC2A


title: 27GOBMUC2H


title: 19GOBMUC2H


title: 26GOBMUC2H


title: 18GOBMUC2A


title: 23GOBMUC2H


title: 12GOBMUC2A


title: 27SMOKEH


title: 09EXPLANTH


title: 10EXPLANTH


title: 57SageA


title: 45SMOKES


title: 57SageA2

value: Asthmatic after Placebo


title: 55SageA


title: 20GOBMUC2H


title: 55SageA2


title: 53SageA


title: 53SageA2

value: Asthmatic after Flovent


title: 56SageA


title: 56SageA2


title: 50SageA


title: 48SAGEA


title: 48SAGEA2


title: 47SAGEA


title: 41SAGEA2


title: 50SageA2


title: 40SMOKEH


title: 40SAGEA2


title: 43SAGEA


title: 36SAGEA2


title: 38SAGEA2


title: 47SAGEA2


title: 39SAGEA


title: 41SAGEA


title: 36SAGEA


title: 29SAGEA


title: 32SAGEA2


title: 43SAGEA2


title: 31SAGEA2


title: 38SAGEA


title: 29SAGEA2


title: 32SAGEA


title: 40SAGEA


title: 27SAGEA


title: 39SAGEA2


title: 31SAGEA


title: 27SAGEA2


title: 25SAGEA2


title: 25SAGEA


title: 18SAGEA


title: 16SAGEA2


title: 16SAGEA


title: 19SAGEA2


title: 18SAGEA2


title: 15SAGEA2


title: 10SAGEA2


title: 20SAGEA2


title: 09SAGEA2


title: 20SAGEA


title: 15SAGEA


title: 11SAGEA2


title: 06SAGEA2


title: 19SAGEA


title: 11SAGEA


title: 09SAGEA


title: 05SAGEA2


title: 05SAGEA


title: 03SAGEA


title: 10SAGEA


title: 03SAGEA2


title: 04SAGEA2


title: 02SAGEA


title: 06SAGEA


title: 02SAGEA2


title: 04SAGEA


title: 01SAGEA2


title: 01SAGEA