title: MDA-MB-468--5min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-7


title: MDA-MB-468--5min-SFM--rep2

status: Public on Jul 21 2016

type: protein

city: Pittsburgh

first: Alison

middle: M

last: Nagle

state: PENNSYLVANIA

address: 204 Craft Ave

department: Pharmacology and Chemical Biology

country: USA

institute: University of Pittsburgh

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-2

hyb_protocol: The diluted lysates were arrayed on nitrocellulose-coated FAST slides. Each slide was probed with a primary antibody and a biotin-conjugated secondary antibody.

scan_protocol: Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction. Slides were scanned on a flatbed scanner to produce 16-bit tiff image. Spots from tiff images were identified and the density was quantified by MicroVigene.

data_processing:
Relative protein levels for each sample were determined by interpolation of each dilution curves from the standard curve (supercurve) of the slide (antibody).
Supercurve is constructed by a script in R written by Bioinformatics.  These values (given as log2 values) are defined as Supercurve Log 2 value  (format reported in the Metadata). All the data points were normalized for protein loading and transformed to linear value designated as Normalized Linear.

label: biotin

molecule: protein

organism: Homo sapiens

source_name: breast cancer

growth_protocol: The cell lines were cultured in DMEM, DMEM/F-12, RPMI, L15 or McCoys 5A media supplemented with 10% FBS or horse serum according to the conditions provided by Neve et al. (2006).

extract_protocol: Cell lysates were harvested by washing in ice-cold PBS twice, and lysing immediately in RPPA lysis buffer with protease inhibitor (1% Triton X-100, 50mM Hepes, 150mM NaCl, 1.5mM MgCl2, 1mM EGTA, 100mM NaF, 10mM NaPPi, 10% Glycerol, 1mM Na3VO4, 1mM PMSF, 10g/ml Aprotinin). Lysates were sonicated and centrifuged at 14,000 rpm at 4oC for 10 min. Protein concentrations were determined by BCA. Then, lysates were mixed with 4X SDS/2-ME sample buffer to have final concentration of 1μg/ml. The lysates were boiled for 5 min and diluted two-fold five times.

treatment_protocol: Cells were plated in triplicate 6cm dishes per treatment time point and then switched to serum-free medium (SFM). Cells were serum starved for 24 hours, then treated with 10nM IGF1 or Ins for 5m, 10m, 30m, 6h, 24h, and 48h.

tag: cell line


title: MDA-MB-453--48hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-45


title: MDA-MB-468--5min-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-1


title: MDA-MB-453--48hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-40


title: MDA-MB-453--48hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-41


title: MDA-MB-453--48hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-39


title: MDA-MB-453--48hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-42


title: MDA-MB-453--48hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-44


title: MDA-MB-453--48hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-43


title: MDA-MB-453--48hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-38


title: MDA-MB-453--48hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-37


title: MDA-MB-453--24hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-34


title: MDA-MB-453--24hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-36


title: MDA-MB-453--24hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-33


title: MDA-MB-453--24hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-28


title: MDA-MB-453--24hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-31


title: MDA-MB-453--24hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-29


title: MDA-MB-453--24hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-30


title: MDA-MB-453--6hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-27


title: MDA-MB-453--6hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-26


title: MDA-MB-453--6hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-23


title: MDA-MB-453--6hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-25


title: MDA-MB-453--6hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-22


title: MDA-MB-453--30min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-19


title: MDA-MB-453--30min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-20


title: MDA-MB-453--30min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-18


title: MDA-MB-453--30min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-21


title: MDA-MB-453--30min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-17


title: MDA-MB-453--30min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-16


title: MDA-MB-453--10min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-13


title: MDA-MB-453--24hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-35


title: MDA-MB-453--10min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-12


title: MDA-MB-453--10min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-11


title: MDA-MB-453--24hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-32


title: MDA-MB-453--10min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-14


title: MDA-MB-453--10min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-10


title: MDA-MB-453--5min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-9


title: MDA-MB-453--10min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-15


title: MDA-MB-453--6hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-24


title: MDA-MB-453--5min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-7


title: MDA-MB-453--5min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-6


title: MDA-MB-453--5min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-5


title: MDA-MB-453--5min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-8


title: MDA-MB-453--5min-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-2


title: MDA-MB-453--5min-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-1


title: MDA-MB-453--5min-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-3


title: MDA-MB-435--48hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-45


title: MDA-MB-435--48hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-43


title: MDA-MB-435--48hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-41


title: MDA-MB-435--48hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-40


title: MDA-MB-435--48hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-44


title: MDA-MB-435--48hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-42


title: MDA-MB-435--24hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-36


title: MDA-MB-453--5min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-453-4


title: MDA-MB-435--48hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-39


title: MDA-MB-435--48hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-38


title: MDA-MB-435--48hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-37


title: MDA-MB-435--24hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-34


title: MDA-MB-435--24hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-33


title: MDA-MB-435--24hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-32


title: MDA-MB-435--24hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-30


title: MDA-MB-435--24hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-35


title: MDA-MB-435--6hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-27


title: MDA-MB-435--24hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-29


title: MDA-MB-435--6hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-25


title: MDA-MB-435--6hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-24


title: MDA-MB-435--6hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-23


title: MDA-MB-435--30min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-21


title: MDA-MB-435--30min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-20


title: MDA-MB-435--30min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-19


title: MDA-MB-435--30min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-17


title: MDA-MB-435--30min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-18


title: MDA-MB-435--6hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-22


title: MDA-MB-435--5min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-9


title: MDA-MB-435--30min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-16


title: MDA-MB-435--10min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-12


title: MDA-MB-435--10min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-10


title: MDA-MB-435--10min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-14


title: MDA-MB-435--24hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-31


title: MDA-MB-435--24hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-28


title: MDA-MB-435--10min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-15


title: MDA-MB-435--5min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-7


title: MDA-MB-435--5min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-5


title: MDA-MB-435--6hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-26


title: MDA-MB-435--5min-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-3


title: MDA-MB-435--5min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-4


title: MDA-MB-435--5min-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-2


title: MDA-MB-415--48hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-44


title: MDA-MB-435--5min-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-1


title: MDA-MB-415--48hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-45


title: MDA-MB-415--48hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-42


title: MDA-MB-415--48hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-37


title: MDA-MB-415--48hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-43


title: MDA-MB-415--48hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-40


title: MDA-MB-415--48hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-41


title: MDA-MB-435--10min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-13


title: MDA-MB-435--5min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-6


title: MDA-MB-415--24hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-36


title: MDA-MB-415--24hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-35


title: MDA-MB-415--48hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-39


title: MDA-MB-435--5min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-8


title: MDA-MB-435--10min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-435-11


title: MDA-MB-415--24hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-32


title: MDA-MB-415--48hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-38


title: MDA-MB-415--24hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-34


title: MDA-MB-415--6hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-27


title: MDA-MB-415--24hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-29


title: MDA-MB-415--6hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-25


title: MDA-MB-415--24hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-31


title: MDA-MB-415--6hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-23


title: MDA-MB-415--6hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-24


title: MDA-MB-415--24hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-33


title: MDA-MB-415--30min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-21


title: MDA-MB-415--24hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-30


title: MDA-MB-415--30min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-20


title: MDA-MB-415--30min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-17


title: MDA-MB-415--30min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-19


title: MDA-MB-415--30min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-18


title: MDA-MB-415--24hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-28


title: MDA-MB-415--6hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-26


title: MDA-MB-415--6hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-22


title: MDA-MB-415--30min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-16


title: MDA-MB-415--10min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-15


title: MDA-MB-415--10min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-14


title: MDA-MB-415--10min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-11


title: MDA-MB-415--10min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-12


title: MDA-MB-415--5min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-8


title: MDA-MB-415--5min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-9


title: MDA-MB-415--5min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-6


title: MDA-MB-415--5min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-4


title: MDA-MB-415--5min-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-3


title: MDA-MB-415--5min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-7


title: MDA-MB-415--5min-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-1


title: MDA-MB-415--5min-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-2


title: MDA-MB-361--48hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-45


title: MDA-MB-361--48hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-42


title: MDA-MB-361--48hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-44


title: MDA-MB-361--48hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-43


title: MDA-MB-361--48hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-40


title: MDA-MB-361--24hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-36


title: MDA-MB-361--48hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-38


title: MDA-MB-361--48hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-37


title: MDA-MB-415--10min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-13


title: MDA-MB-361--24hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-35


title: MDA-MB-415--10min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-10


title: MDA-MB-361--24hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-34


title: MDA-MB-415--5min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-415-5


title: MDA-MB-361--24hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-30


title: MDA-MB-361--24hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-29


title: MDA-MB-361--24hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-32


title: MDA-MB-361--24hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-31


title: MDA-MB-361--6hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-27


title: MDA-MB-361--24hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-28


title: MDA-MB-361--6hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-26


title: MDA-MB-361--6hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-25


title: MDA-MB-361--48hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-41


title: MDA-MB-361--6hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-24


title: MDA-MB-361--6hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-23


title: MDA-MB-361--30min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-20


title: MDA-MB-361--6hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-22


title: MDA-MB-361--30min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-21


title: MDA-MB-361--30min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-18


title: MDA-MB-361--30min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-16


title: MDA-MB-361--30min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-19


title: MDA-MB-361--30min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-17


title: MDA-MB-361--48hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-39


title: MDA-MB-361--10min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-14


title: MDA-MB-361--10min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-15


title: MDA-MB-361--24hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-33


title: MDA-MB-361--10min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-12


title: MDA-MB-361--10min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-13


title: MDA-MB-361--10min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-11


title: MDA-MB-361--5min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-9


title: MDA-MB-361--10min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-10


title: MDA-MB-361--5min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-7


title: MDA-MB-361--5min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-8


title: MDA-MB-361--5min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-5


title: MDA-MB-361--5min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-6


title: MDA-MB-361--5min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-4


title: MDA-MB-361--5min-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-2


title: MDA-MB-361--5min-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-3


title: MDA-MB-361--5min-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-361-1


title: MDA-MB-231--48hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-42


title: MDA-MB-231--48hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-45


title: MDA-MB-231--48hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-40


title: MDA-MB-231--48hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-41


title: MDA-MB-231--48hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-39


title: MDA-MB-231--48hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-38


title: MDA-MB-231--48hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-37


title: MDA-MB-231--24hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-36


title: MDA-MB-231--24hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-35


title: MDA-MB-231--24hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-34


title: MDA-MB-231--48hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-44


title: MDA-MB-231--24hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-33


title: MDA-MB-231--24hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-32


title: MDA-MB-231--48hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-43


title: LNCAP_72h_Treatment 2_v_Control 2

type: RNA

city: Seattle

first: Robert

last: Moritz

state: Washington

address: 401 Terry Ave Nth

institute: Institute for Systems Biology

hyb_protocol: Microarray experiments were carried out using whole human genome oligo arrays with 8x60k 60-mer probes (Agilent Technologies) with 25 ng total RNA starting material according to the manufacturer’s protocol

scan_protocol: Hybridized arrays were scanned with Agilent’s dual laser-based scanner. Feature Extraction software version 10.5 (Agilent Technologies) was used to link a feature to a design file and to determine the relative fluorescence intensity between two samples.

data_processing:
Array raw data were imported into BRB Array Tools version 4.3.0 for further analysis. Lowess normalization was applied to each array. The ratios of the average Lowess normalized gene expression values of treated versus control samples for each time point of each cancer cell line were used to determine differential gene expression over time.
Genes with absolute fold-change ≥2 at least in one time point of one cancer cell line were hierarchically clustered using clustergram MATLAB function with Euclidean distance metric and average linkage.

molecule: total RNA

source_name: Prostate cancer cell line

label_protocol: Dye swap strategy with alternate cy3 and cy5 labeling on docetaxel treated and control groups over four time points was used to have technical replicates and decrease dye bias.

growth_protocol: DU145 (ATCC HTB-81), LNCaP clone FGC (ATCC CRl-1740) and PC-3 (ATCC CRL-1435) cells were cultured at 37° C with 5% C02 in EMEM, RPMI-1640 and F-12K medium, respectively. Cell culture medium was supplemented with 10% FBS. Cells were tested for mycoplasma contamination.

extract_protocol: Total RNA was extracted from docetaxel treated cells and untreated controls at each time point and cell line using the RNeasy mini kit (Qiagen), cells for microarray analysis were from an equivalent preparation than for SRM analysis. Total RNA was treated on-column with RNase-free DNase (Qiagen). RNA quality was assessed with an Agilent 2100 Bioanalyzer to ensure RNA integrity.

treatment_protocol: Cells were allowed to incubate 48h prior to treatment with docetaxel (Biotang Inc.). Docetaxel was dissolved in ethanol and diluted in media. For the treated condition, DU145 was incubated with media containing 9 nM docetaxel, PC-3 with 10 nM and LNCaP with 3.5 nM (previously determined IC50 concentration for each cell line; LNCaP cells treated with 10 nM docetaxel showed visual damage by 8h and all cells died within 48h). For time point controls, cells were cultured in media containing an equal amount of ethanol (0.005%). Cells were harvested at 0h and treated and control cells at 8, 24, 48 and 72h post treatment.

value: LNCAP

tag: time point

value: 72 hour

tag: agent

value: docetaxel

value: Control


title: LNCAP_48h_Treatment 2_v_Control 2

value: 48 hour


title: LNCAP_24h_Treatment 2_v_Control 2

value: 24 hour


title: DU145_24h_Treatment 1_v_Control 1


title: DU145_24h_Treatment 2_v_Control 2


title: DU145_48h_Treatment 2_v_Control 2


title: LNCAP_72h_Treatment 1_v_Control 1


title: LNCAP_8h_Treatment 1_v_Control 1

value: 8 hour


title: LNCAP_8h_Treatment 2_v_Control 2


title: LNCAP_48h_Treatment 1_v_Control 1


title: DU145_72h_Treatment 1_v_Control 1


title: DU145_8h_Treatment 2_v_Control 2


title: DU145_72h_Treatment 2_v_Control 2


title: DU145_8h_Treatment 1_v_Control 1


title: LNCAP_24h_Treatment 1_v_Control 1


title: MDA-MB-231--24hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-31


title: MDA-MB-231--24hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-29


title: MDA-MB-231--24hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-28


title: MDA-MB-231--24hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-30


title: MDA-MB-231--6hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-27


title: PC3_72h_Treatment 1_v_Control 1


title: DU145_48h_Treatment 1_v_Control 1


title: MDA-MB-231--6hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-25


title: MDA-MB-231--6hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-26


title: PC3_72h_Treatment 2_v_Control 2


title: MDA-MB-231--6hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-22


title: MDA-MB-231--6hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-23


title: MDA-MB-231--30min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-21


title: MDA-MB-231--30min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-17


title: MDA-MB-231--30min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-16


title: MDA-MB-231--10min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-15


title: PC3_48h_Treatment 1_v_Control 1


title: MDA-MB-231--30min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-20


title: PC3_8h_Treatment 2_v_Control 2


title: PC3_24h_Treatment 1_v_Control 1


title: PC3_24h_Treatment 2_v_Control 2


title: MDA-MB-231--30min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-19


title: MDA-MB-231--30min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-18


title: MDA-MB-231--10min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-14


title: MDA-MB-231--6hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-24


title: MDA-MB-231--10min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-12


title: PC3_8h_Treatment 1_v_Control 1


title: T1444Sp.AVG_Signal

city: Oslo

first: Biljana

last: Stangeland

address: Sognsvannsveien 20

department: Institute of Surgical Research

country: Norway

institute: Oslo University Hospital

description:
Sp-Mes 4  (GBM sphere cultures)
biological replicate 4
T1444Sp

hyb_protocol: Standard Illumina hybridization protocol

scan_protocol: Standard Illumina scanning protocol

data_processing: The data were imported to gene expression analysis software “JExpress” (Molmine, Bergen, Norway) http://jexpress.bioinfo.no) and  normalised using quantile normalisation

source_name: patient cells growing adherently in sphere conditons

label_protocol: Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays

extract_protocol: RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.

tag: cell/tissue type

value: GBM sphere cultures

tag: culture type

value: Grown as spheres


title: MDA-MB-231--10min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-11


title: MDA-MB-231--5min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-7


title: MDA-MB-231--5min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-8


title: MDA-MB-231--5min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-6


title: MDA-MB-231--5min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-5


title: MDA-MB-231--5min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-9


title: MDA-MB-231--10min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-13


title: MDA-MB-231--10min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-10


title: T1370Sp.AVG_Signal

description:
Sp-Mes 2  (GBM sphere cultures)
biological replicate 2
T1370Sp


title: MDA-MB-231--5min-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-2


title: MDA-MB-134--48hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-45


title: T1344Sp.AVG_Signal

description:
Sp-Mes 3  (GBM sphere cultures)
biological replicate 3
T1344Sp


title: MDA-MB-231--5min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-4


title: T1403Sp.AVG_Signal

description:
Sp-Mes 1  (GBM sphere cultures)
biological replicate 1
T1403Sp


title: MDA-MB-231--5min-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-1


title: MDA-MB-134--48hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-43


title: MDA-MB-134--48hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-44


title: PC3_48h_Treatment 2_v_Control 2


title: MDA-MB-231--5min-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-231-3


title: 11_Sp_T1349

description:
Sp 3  (GBM sphere cultures)
biological replicate 3
11_Sp_T1349

source_name: patient cells growing as spheres


title: 43_Sp_T1402

description:
Sp 5  (GBM sphere cultures)
biological replicate 5
43_Sp_T1402


title: 35_AT

description:
AT-MSC (adipose tissue mesenchymal stem cells)
biological replicate 1
35_AT

source_name: patient cells growing as adherent monoleyer

value: Adipose tissue mesenchymal stem cells

value: adipose tissue-MSC


title: MDA-MB-134--48hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-41


title: 27_Sp_T1341

description:
Sp 4  (GBM sphere cultures)
biological replicate 4
27_Sp_T1341


title: 3_Sp_T1354

description:
Sp 1 (GBM sphere cultures)
biological replicate 1
3_Sp_T1354


title: 19_Sp_T1348

description:
Sp 2  (GBM sphere cultures)
biological replicate 2
19_Sp_T1348


title: 33_BM

description:
BM-MSC (bone marrow mesenchymal stem cells)
biological replicate 1
33_BM

value: Bone marrow mesenchymal stem cells

value: bone marrow-MSC


title: T1444Frfast.AVG_Signal

description:
Fr-Mes 4 (fresh GBM tissue)
biological replicate 4
T1444Frfast

source_name: fresh tissues tripsinized from glioblastoma patient (sample with mesenchymal properties)

value: fresh GBM tissue


title: 39_AD1_H1401

description:
Mes (adult human brain cell culture in 1% serum)
biological replicate 1
39_AD1_H1401

value: control normal in 1% FBS+TGF-a


title: MDA-MB-134--48hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-40


title: MDA-MB-134--48hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-39


title: MDA-MB-134--48hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-38


title: MDA-MB-134--24hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-36


title: MDA-MB-134--48hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-42


title: T1344Frfast.AVG_Signal

description:
Fr-Mes 3 (fresh GBM tissue)
biological replicate 3
T1344Frfast


title: MDA-MB-134--48hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-37


title: MDA-MB-134--24hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-34


title: MDA-MB-134--24hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-32


title: MDA-MB-134--24hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-31


title: 37_Fr_H1401

description:
Fr-brain (fresh normal human brain  tissue)
biological replicate 2
37_Fr_H1401

source_name: fresh tissues tripsinized from epileptic patient

value: fresh Normal human brain tissue


title: MDA-MB-134--24hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-30


title: MDA-MB-134--24hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-29


title: MDA-MB-134--24hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-28


title: 17_Fr_1348

description:
Fr 3 (fresh GBM tissue)
biological replicate 3
17_Fr_1348

source_name: fresh tissues tripsinized from glioblastoma patient


title: T1403Fr.AVG_Signal

description:
Fr-Mes 1 (fresh GBM tissue)
biological replicate 1
T1403Fr


title: T1370Frfast.AVG_Signal

description:
Fr-Mes 2 (fresh GBM tissue)
biological replicate 2
T1370Frfast


title: MDA-MB-134--24hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-35


title: 25_Fr_T1341

description:
Fr 4 (fresh GBM tissue)
biological replicate 4
25_Fr_T1341


title: 23_AD10_T1348

description:
AD10 3  (adherent cell culture in 10% serum)
biological replicate 3
23_AD10_T1348

value: GBM adherent cell culture in 10% serum

value: adherent 10% FBS


title: 47_AD10_T1402

description:
AD10 1 (adherent cell culture in 10% serum)
biological replicate 1
47_AD10_T1402


title: 1_Fr_T1354

description:
Fr 1 (fresh GBM tissue)
biological replicate 1
1_Fr_T1354


title: MDA-MB-134--24hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-33


title: 31_AD10_T1341

description:
AD10 4  (adherent cell culture in 10% serum)
biological replicate 4
31_AD10_T1341


title: MDA-MB-134--6hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-27


title: 9_Fr_T1349

description:
Fr 2 (fresh GBM tissue)
biological replicate 2
9_Fr_T1349


title: 7_AD10_T1354

description:
AD10 2  (adherent cell culture in 10% serum)
biological replicate 2
7_AD10_T1354


title: 41C_Fr_T1402

description:
Fr 5 (fresh GBM tissue)
biological replicate 5
41_Fr_T1402


title: 13_AD1_T1349

description:
AD1 4 (adherent cell culture in 1% serum)
biological replicate 4
13_AD1_T1349

value: GBM adherent cell culture in 1% serum

value: adherent 1% FBS+TGF-a


title: T1370AD1.AVG_Signal

description:
AD1 6 (adherent cell culture in 1% serum)
biological replicate 6
T1370AD1


title: MDA-MB-134--6hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-26


title: 29_AD1_T1341

description:
AD1 5 (adherent cell culture in 1% serum)
biological replicate 5
29_AD1_T1341


title: MDA-MB-134--6hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-24


title: MDA-MB-134--6hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-23


title: MDA-MB-134--30min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-20


title: MDA-MB-134--6hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-22


title: MDA-MB-134--6hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-25


title: MDA-MB-134--30min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-21


title: MDA-MB-134--30min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-18


title: MDA-MB-134--30min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-17


title: MDA-MB-134--30min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-16


title: MDA-MB-134--10min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-15


title: MDA-MB-134--10min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-14


title: MDA-MB-134--10min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-12


title: MDA-MB-134--30min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-19


title: MDA-MB-134--10min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-13


title: 45_AD1_T1402

description:
AD1 1 (adherent cell culture in 1% serum)
biological replicate 1
45_AD1_T1402


title: 5_AD1_T1354

description:
AD1 2 (adherent cell culture in 1% serum)
biological replicate 2
5_AD1_T1354


title: MDA-MB-134--10min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-10


title: MDA-MB-134--10min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-11


title: 21_AD1_T1348

description:
AD1 3 (adherent cell culture in 1% serum)
biological replicate 3
21_AD1_T1348


title: 15_AD10_T1349

description:
AD-Mix 1 (mixture of cells from adherent  and sphere cultured)
biological replicate 1
15_AD10_T1349

value: GBM cell culture in 10% serum; mixture of cells from adherent and sphere cultured

value: adherent in 10% FBS


title: T1341AD10M.AVG_Signal

description:
AD-Mix 3 (mixture of cells from adherent  and sphere cultured)
biological replicate 3
T1341AD10M


title: T1344AD10.AVG_Signal

description:
AD-Mix 4 (mixture of cells from adherent  and sphere cultured)
biological replicate 4
T1344AD10


title: MDA-MB-134--5min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-8


title: MDA-MB-134--5min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-9


title: T1403AD10.AVG_Signal

description:
AD-Mix 2 (mixture of cells from adherent  and sphere cultured)
biological replicate 2
T1403AD1

value: GBM cell culture in 1% serum; mixture of cells from adherent and sphere cultured

value: adherent in 1% FBS+TGF-a


title: MDA-MB-134--5min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-6


title: MDA-MB-134--5min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-5


title: MDA-MB-134--5min-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-3


title: MDA-MB-134--5min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-4