title: C-NNa35

city: Little Rock

first: Intawat

last: Nookaew

address: 4301 W Markham St

department: Department of Biomedical Informatics

institute: University of Arkansas for Medical Sciences

hyb_protocol: Fragmented aRNA was hybridized to Rat230 Genome 2.0 arrays (Affymetrix), containing 31 000 transcripts.

scan_protocol: The arrays were washed and processed on a Fluidics Station 450 and scanned with a confocal laser scanner (GeneChip Scanner 3000, Affymetrix). The scanned images (.DAT files) were converted into .CEL files by using the Command console software (Affymetrix).

data_processing: The raw expression signals were preprocessed following the method of Probe Logarithmic Intensity Error (PLIER) based on perfect match only (pm-only) calculation (Affymetrix).The robust quantile method was applied to get normalized expression values

label: Biotin

organism: Rattus norvegicus

source_name: thoracic aorta

label_protocol: 100 ng of purified RNA was reverse transcribed and labeled using the GeneChip 3’ IVT Express Kit, which utilizes an oligo dT based reverse transcription reaction followed by a T7 promoted in-vitro transcription biotin labeling reaction (Affymetrix, Santa Clara, CA).

extract_protocol: Total RNA was prepared using the RNeasy Fibrous Tissue Mini Kit (Qiagen).

treatment_protocol: Tissues were immersed in RNAlater overnight in 4°C and then flash-frozen in liquid nitrogen for -80°C storage.

tag: treatment

value: Control

tag: tissue

value: thoracic aorta

tag: strain

value: Sprague-Dawley


title: C-NNa37

status: Public on Jul 21 2016

type: RNA

country: USA

molecule: total RNA


title: MDA-MB-134--5min-SFM--rep2

type: protein

city: Pittsburgh

first: Alison

middle: M

last: Nagle

state: PENNSYLVANIA

address: 204 Craft Ave

department: Pharmacology and Chemical Biology

institute: University of Pittsburgh

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-2

hyb_protocol: The diluted lysates were arrayed on nitrocellulose-coated FAST slides. Each slide was probed with a primary antibody and a biotin-conjugated secondary antibody.

scan_protocol: Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction. Slides were scanned on a flatbed scanner to produce 16-bit tiff image. Spots from tiff images were identified and the density was quantified by MicroVigene.

data_processing:
Relative protein levels for each sample were determined by interpolation of each dilution curves from the standard curve (supercurve) of the slide (antibody).
Supercurve is constructed by a script in R written by Bioinformatics.  These values (given as log2 values) are defined as Supercurve Log 2 value  (format reported in the Metadata). All the data points were normalized for protein loading and transformed to linear value designated as Normalized Linear.

label: biotin

molecule: protein

organism: Homo sapiens

source_name: breast cancer

growth_protocol: The cell lines were cultured in DMEM, DMEM/F-12, RPMI, L15 or McCoys 5A media supplemented with 10% FBS or horse serum according to the conditions provided by Neve et al. (2006).

extract_protocol: Cell lysates were harvested by washing in ice-cold PBS twice, and lysing immediately in RPPA lysis buffer with protease inhibitor (1% Triton X-100, 50mM Hepes, 150mM NaCl, 1.5mM MgCl2, 1mM EGTA, 100mM NaF, 10mM NaPPi, 10% Glycerol, 1mM Na3VO4, 1mM PMSF, 10g/ml Aprotinin). Lysates were sonicated and centrifuged at 14,000 rpm at 4oC for 10 min. Protein concentrations were determined by BCA. Then, lysates were mixed with 4X SDS/2-ME sample buffer to have final concentration of 1μg/ml. The lysates were boiled for 5 min and diluted two-fold five times.

treatment_protocol: Cells were plated in triplicate 6cm dishes per treatment time point and then switched to serum-free medium (SFM). Cells were serum starved for 24 hours, then treated with 10nM IGF1 or Ins for 5m, 10m, 30m, 6h, 24h, and 48h.

tag: cell line


title: MCF10A--48hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-45


title: MCF10A--48hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-44


title: C-NNa36


title: MCF10A--48hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-43


title: C-NNa38


title: C-NNa34


title: A-NNa18

value: A-CRF


title: C-NNa33


title: MCF10A--48hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-42


title: A-NNa13


title: A-NNa16


title: A-NNa17


title: A-NNa11


title: A-NNa14


title: A-NNa10


title: MCF10A--48hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-41


title: MDA-MB-134--5min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-7


title: MDA-MB-134--5min-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-134-1


title: MCF10A--48hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-40


title: Before abatacept administration, Remission-6

city: Minato-ku

first: Seiji

last: Nakamura

state: Tokyo

address: 1-15-1, Kaigan, Suzuebaydium 5F

country: Japan

institute: DNA Chip Research Inc.

hyb_protocol: Agilent one-color gene expression hyb/wash protocol

scan_protocol: Slides were scanned immediately after washing on an Agilent DNA Microarray Scanner using one color scan setting for 1x44k array slides.

data_processing: The scanned images were analyzed with Feature Extraction Software (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. We applied rank-based quantile normalization to the raw signal data.

source_name: Whole blood, Rheumatoid arthritis, Before abatacept administration, Remission

label_protocol: Agilent one-color Quick Amp Labeling Kit labeling protocol

extract_protocol: RNA was prepared using the PAXgene Blood RNA Kit (QIAGEN) according to the manufacture's instructions.

treatment_protocol: Before administration with biologic agent, blood samples were collected in PAXgene Blood RNA tubes.

value: Whole blood

tag: disease

value: Rheumatoid arthritis

tag: sampling point

value: Before abatacept administration

tag: clinical outcome

value: Remission

tag: gender

value: Female

tag: age


title: Before abatacept administration, Remission-5


title: Before abatacept administration, Remission-7


title: MCF10A--48hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-37


title: MCF10A--48hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-38


title: MCF10A--48hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-39


title: MCF10A--24hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-36


title: MCF10A--24hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-35


title: MCF10A--24hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-33


title: MCF10A--24hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-34


title: MCF10A--24hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-30


title: MCF10A--24hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-29


title: MCF10A--24hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-31


title: MCF10A--24hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-28


title: MCF10A--6hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-27


title: MCF10A--6hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-26


title: Before abatacept administration, Remission-4


title: MCF10A--24hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-32


title: Before abatacept administration, Non-remission-23

source_name: Whole blood, Rheumatoid arthritis, Before abatacept administration, Non-remission

value: Non-remission


title: Before abatacept administration, Remission-3

value: Male


title: MCF10A--6hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-24


title: MCF10A--6hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-25


title: MCF10A--6hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-23


title: Before abatacept administration, Non-remission-24


title: Before abatacept administration, Non-remission-21


title: Before abatacept administration, Non-remission-18


title: Before abatacept administration, Remission-2


title: Before abatacept administration, Non-remission-20


title: Before abatacept administration, Non-remission-17


title: Before abatacept administration, Non-remission-15


title: Before abatacept administration, Non-remission-16


title: Before abatacept administration, Non-remission-22


title: A-NNa15


title: Before abatacept administration, Remission-1


title: MCF10A--6hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-22


title: MCF10A--30min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-20


title: Before abatacept administration, Non-remission-14


title: Before abatacept administration, Non-remission-19


title: Before abatacept administration, Non-remission-12


title: MCF10A--30min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-21


title: Before abatacept administration, Non-remission-13


title: MCF10A--30min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-19


title: Before abatacept administration, Non-remission-9


title: MCF10A--30min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-18


title: Before abatacept administration, Non-remission-10


title: MCF10A--30min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-17


title: MCF10A--30min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-16


title: MCF10A--10min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-15


title: MCF10A--10min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-13


title: MCF10A--10min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-14


title: MCF10A--10min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-12


title: MCF10A--10min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-10


title: MCF10A--10min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-11


title: MCF10A--5min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-9


title: Before abatacept administration, Non-remission-11


title: MCF10A--5min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-7


title: Before abatacept administration, Non-remission-5


title: Before abatacept administration, Non-remission-7


title: MCF10A--5min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-8


title: MCF10A--5min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-6


title: Before abatacept administration, Non-remission-6


title: Before abatacept administration, Non-remission-8


title: MCF10A--5min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-5


title: Before abatacept administration, Non-remission-1


title: Before tocilizumab administration, Remission-6

source_name: Whole blood, Rheumatoid arthritis, Before tocilizumab administration, Remission

value: Before tocilizumab administration


title: MCF10A--5min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-4


title: MCF10A--5min-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-3


title: Before tocilizumab administration, Remission-8


title: Before tocilizumab administration, Remission-7


title: Before tocilizumab administration, Remission-4


title: Before tocilizumab administration, Remission-3


title: Before tocilizumab administration, Remission-2


title: Before tocilizumab administration, Remission-1


title: Before abatacept administration, Non-remission-4


title: Before abatacept administration, Non-remission-3


title: Before abatacept administration, Non-remission-2


title: MCF10A--5min-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-2


title: MCF10A--5min-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF10A-1


title: Before tocilizumab administration, Non-remission-30

source_name: Whole blood, Rheumatoid arthritis, Before tocilizumab administration, Non-remission


title: Before tocilizumab administration, Non-remission-29


title: Before tocilizumab administration, Non-remission-27


title: Before tocilizumab administration, Remission-5


title: MCF7--48hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-45


title: Before tocilizumab administration, Non-remission-28


title: MCF7--48hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-44


title: MCF7--48hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-43


title: MCF7--48hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-41


title: MCF7--48hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-40


title: Before tocilizumab administration, Non-remission-25


title: MCF7--48hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-38


title: Before tocilizumab administration, Non-remission-26


title: Before tocilizumab administration, Non-remission-24


title: MCF7--24hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-36


title: MCF7--24hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-35


title: Before tocilizumab administration, Non-remission-23


title: Before tocilizumab administration, Non-remission-22


title: MCF7--24hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-34


title: MCF7--24hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-33


title: MCF7--24hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-30


title: MCF7--24hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-31


title: Before tocilizumab administration, Non-remission-20


title: Before tocilizumab administration, Non-remission-17


title: Before tocilizumab administration, Non-remission-19


title: Before tocilizumab administration, Non-remission-14


title: MCF7--48hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-37


title: Before tocilizumab administration, Non-remission-18


title: Before tocilizumab administration, Non-remission-15


title: Before tocilizumab administration, Non-remission-16


title: Before tocilizumab administration, Non-remission-21


title: MCF7--48hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-39


title: MCF7--24hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-28


title: MCF7--6hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-27


title: MCF7--6hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-25


title: MCF7--6hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-24


title: MCF7--24hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-29


title: MCF7--6hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-23


title: MCF7--6hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-22


title: Before tocilizumab administration, Non-remission-10


title: MCF7--30min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-21


title: MCF7--30min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-20


title: MCF7--30min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-19


title: Before tocilizumab administration, Non-remission-13


title: MCF7--30min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-18


title: Before tocilizumab administration, Non-remission-7


title: Before tocilizumab administration, Non-remission-5


title: MCF7--6hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-26


title: Before tocilizumab administration, Non-remission-11


title: Before tocilizumab administration, Non-remission-8


title: Before tocilizumab administration, Non-remission-4


title: MCF7--30min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-17


title: Before tocilizumab administration, Non-remission-6


title: Before tocilizumab administration, Non-remission-3


title: Before tocilizumab administration, Non-remission-1


title: Before infliximab administration, Remission-42

source_name: Whole blood, Rheumatoid arthritis, Before infliximab administration, Remission

value: Before infliximab administration


title: Before infliximab administration, Remission-41


title: Before tocilizumab administration, Non-remission-9


title: Before infliximab administration, Remission-40


title: MCF7--30min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-16


title: MCF7--10min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-15


title: MCF7--24hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-32


title: MCF7--10min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-14


title: MCF7--10min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-13


title: MCF7--10min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-12


title: Before infliximab administration, Remission-36


title: MCF7--10min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-11


title: MCF7--10min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-10


title: MCF7--5min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-9


title: MCF7--5min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-7


title: Before infliximab administration, Remission-39


title: MCF7--5min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-8


title: MCF7--5min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-6


title: Before infliximab administration, Remission-37


title: MCF7--5min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-4


title: MCF7--5min-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-3


title: Before tocilizumab administration, Non-remission-2


title: Before infliximab administration, Remission-33


title: Before infliximab administration, Remission-38


title: MCF7--5min-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-1


title: Before infliximab administration, Remission-31


title: Before infliximab administration, Remission-34


title: Before tocilizumab administration, Non-remission-12


title: HS758T--48hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-45


title: Before infliximab administration, Remission-35


title: Before infliximab administration, Remission-30


title: Before infliximab administration, Remission-28


title: Before infliximab administration, Remission-32


title: Before infliximab administration, Remission-29


title: Before infliximab administration, Remission-26


title: Before infliximab administration, Remission-27


title: MCF7--5min-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-2


title: HS758T--48hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-43


title: Before infliximab administration, Remission-24


title: HS758T--48hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-41


title: HS758T--48hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-42


title: HS758T--48hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-40


title: HS758T--48hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-38


title: HS758T--48hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-39


title: Before infliximab administration, Remission-23


title: Before infliximab administration, Remission-22


title: Before infliximab administration, Remission-21


title: Before infliximab administration, Remission-25


title: Before infliximab administration, Remission-20


title: Before infliximab administration, Remission-19


title: HS758T--24hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-35


title: HS758T--24hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-34


title: HS758T--24hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-33


title: Before infliximab administration, Remission-18


title: Before infliximab administration, Remission-15


title: Before infliximab administration, Remission-17


title: Before infliximab administration, Remission-14


title: Before infliximab administration, Remission-16


title: HS758T--24hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-32


title: Before infliximab administration, Remission-11


title: Before infliximab administration, Remission-12


title: Before infliximab administration, Remission-13


title: HS758T--24hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-31


title: HS758T--48hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-37


title: HS758T--24hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-36


title: Before infliximab administration, Remission-9


title: HS758T--24hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-30


title: HS758T--24hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-28


title: HS758T--24hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-29


title: HS758T--6hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-27


title: HS758T--6hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-25


title: HS758T--6hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-24


title: HS758T--6hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-23


title: Before infliximab administration, Remission-8


title: Before infliximab administration, Remission-7


title: Before infliximab administration, Remission-10


title: Before infliximab administration, Remission-6


title: Before infliximab administration, Remission-4


title: HS758T--30min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-20


title: HS758T--30min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-18


title: Before infliximab administration, Remission-5


title: Before infliximab administration, Remission-3


title: Before infliximab administration, Remission-2


title: HS758T--6hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-22


title: HS758T--6hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-26


title: HS758T--30min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-17


title: HS758T--30min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-21


title: Before infliximab administration, Remission-1


title: HS758T--30min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-19


title: HS758T--10min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-15


title: HS758T--30min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-16


title: HS758T--10min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-14


title: HS758T--10min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-13


title: HS758T--10min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-12


title: HS758T--10min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-11


title: HS758T--10min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-10


title: HS758T--5min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-9


title: HS758T--5min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-7


title: HS758T--5min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-8


title: Before infliximab administration, Non-remission-95

source_name: Whole blood, Rheumatoid arthritis, Before infliximab administration, Non-remission


title: Before infliximab administration, Non-remission-93


title: Before infliximab administration, Non-remission-96


title: Before infliximab administration, Non-remission-98


title: Before infliximab administration, Non-remission-97


title: Before infliximab administration, Non-remission-90


title: Before infliximab administration, Non-remission-87


title: Before infliximab administration, Non-remission-91


title: Before infliximab administration, Non-remission-94


title: Before infliximab administration, Non-remission-88


title: Before infliximab administration, Non-remission-84


title: Before infliximab administration, Non-remission-89


title: Before infliximab administration, Non-remission-83


title: Before infliximab administration, Non-remission-86


title: HS758T--5min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-6


title: HS758T--5min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-5


title: Before infliximab administration, Non-remission-82


title: HS758T--5min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-4


title: HS758T--5min-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-3


title: HS758T--5min-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-2


title: Before infliximab administration, Non-remission-81


title: HS758T--5min-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-1


title: HCC1569--48hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-45


title: Before infliximab administration, Non-remission-85


title: HCC1569--48hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-44


title: Before infliximab administration, Non-remission-79


title: HCC1569--48hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-43


title: Before infliximab administration, Non-remission-80


title: Before infliximab administration, Non-remission-77


title: Before infliximab administration, Non-remission-78


title: HCC1569--48hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-42


title: HCC1569--48hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-40


title: Before infliximab administration, Non-remission-75


title: Before infliximab administration, Non-remission-76


title: HCC1569--48hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-41


title: MCF7--48hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-42


title: Before infliximab administration, Non-remission-74


title: Before infliximab administration, Non-remission-70


title: Before infliximab administration, Non-remission-73


title: Before infliximab administration, Non-remission-72


title: HCC1569--24hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-36


title: HCC1569--48hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-39


title: Before infliximab administration, Non-remission-71


title: HCC1569--48hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-37


title: HCC1569--24hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-35


title: HCC1569--24hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-34


title: HCC1569--24hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-32


title: HCC1569--24hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-33


title: HCC1569--24hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-31


title: Before infliximab administration, Non-remission-67


title: HCC1569--24hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-30


title: Before infliximab administration, Non-remission-68


title: HCC1569--24hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-28


title: HCC1569--24hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-29


title: HCC1569--6hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-27


title: Before infliximab administration, Non-remission-65


title: HCC1569--6hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-26


title: Before infliximab administration, Non-remission-66


title: Before infliximab administration, Non-remission-63


title: HCC1569--6hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-25


title: Before infliximab administration, Non-remission-69


title: Before infliximab administration, Non-remission-61


title: HCC1569--6hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-24


title: HCC1569--6hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-23


title: Before infliximab administration, Non-remission-60


title: Before infliximab administration, Non-remission-59


title: Before infliximab administration, Non-remission-64


title: Before infliximab administration, Non-remission-58


title: HCC1569--6hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-22


title: Before infliximab administration, Non-remission-62


title: Before infliximab administration, Non-remission-55


title: Before infliximab administration, Non-remission-56


title: HCC1569--30min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-20


title: Before infliximab administration, Non-remission-57


title: HCC1569--30min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-19


title: HCC1569--30min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-18


title: HCC1569--30min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-17


title: HCC1569--10min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-15


title: HCC1569--30min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-16


title: HCC1569--30min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-21


title: Before infliximab administration, Non-remission-51


title: Before infliximab administration, Non-remission-54


title: HCC1569--10min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-13


title: Before infliximab administration, Non-remission-53


title: HCC1569--10min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-14


title: Before infliximab administration, Non-remission-50


title: Before infliximab administration, Non-remission-52


title: HCC1569--10min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-12


title: HCC1569--10min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-10


title: HCC1569--10min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-11


title: Before infliximab administration, Non-remission-46


title: HCC1569--5min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-8


title: Before infliximab administration, Non-remission-48


title: HCC1569--5min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-9


title: Before infliximab administration, Non-remission-47


title: HCC1569--5min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-7


title: Before infliximab administration, Non-remission-42


title: Before infliximab administration, Non-remission-45


title: Before infliximab administration, Non-remission-40


title: Before infliximab administration, Non-remission-44


title: Before infliximab administration, Non-remission-41


title: Before infliximab administration, Non-remission-43


title: HCC1569--5min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-6


title: HCC1569--5min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-5


title: Before infliximab administration, Non-remission-39


title: HCC1569--5min-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-3


title: HCC1569--5min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-4


title: Before infliximab administration, Non-remission-37


title: Before infliximab administration, Non-remission-38


title: HCC1569--5min-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-1


title: HCC1569--5min-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-2


title: Before infliximab administration, Non-remission-36


title: HCC1954--48hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-45


title: HCC1954--48hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-44


title: Before infliximab administration, Non-remission-34


title: Before infliximab administration, Non-remission-33


title: HCC1954--48hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-43


title: Before infliximab administration, Non-remission-35


title: Before infliximab administration, Non-remission-31


title: HCC1954--48hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-42


title: Before infliximab administration, Non-remission-30


title: HCC1954--48hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-41


title: HCC1954--48hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-40


title: Before infliximab administration, Non-remission-29


title: HCC1954--48hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-38


title: HCC1954--48hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-39


title: Before infliximab administration, Non-remission-27


title: Before infliximab administration, Non-remission-26


title: HCC1954--48hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-37


title: HCC1954--24hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-36


title: Before infliximab administration, Non-remission-28


title: HCC1954--24hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-35


title: Before infliximab administration, Non-remission-25


title: Before infliximab administration, Non-remission-49


title: HCC1954--24hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-34


title: Before infliximab administration, Non-remission-24


title: HCC1954--24hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-33


title: HCC1954--24hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-32


title: Before infliximab administration, Non-remission-23


title: Before infliximab administration, Non-remission-32


title: HCC1954--24hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-31


title: HCC1954--24hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-29


title: HCC1954--24hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-28


title: HCC1954--24hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-30


title: Before infliximab administration, Non-remission-21


title: Before infliximab administration, Non-remission-18


title: Before infliximab administration, Non-remission-20


title: HCC1954--6hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-27


title: Before infliximab administration, Non-remission-17


title: Before infliximab administration, Non-remission-15


title: Before infliximab administration, Non-remission-19


title: Before infliximab administration, Non-remission-16


title: HCC1954--6hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-26


title: MCF7--5min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MCF7-5


title: HCC1569--48hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1569-38


title: HCC1954--6hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-25


title: HS758T--48hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HS758T-44


title: HCC1954--6hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-23


title: HCC1954--30min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-21


title: HCC1954--6hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-22


title: HCC1954--30min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-20


title: Before infliximab administration, Non-remission-22


title: Before infliximab administration, Non-remission-12


title: Before infliximab administration, Non-remission-10


title: Before infliximab administration, Non-remission-92


title: Before infliximab administration, Non-remission-11


title: HCC1954--30min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-19


title: Before infliximab administration, Non-remission-13


title: Before infliximab administration, Non-remission-14


title: Before infliximab administration, Non-remission-9


title: HCC1954--30min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-17


title: HCC1954--30min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-18


title: Before infliximab administration, Non-remission-8


title: HCC1954--10min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-14


title: HCC1954--30min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-16


title: HCC1954--10min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-13


title: Before infliximab administration, Non-remission-7


title: Before infliximab administration, Non-remission-3


title: Before infliximab administration, Non-remission-5


title: HCC1954--6hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-24


title: Before infliximab administration, Non-remission-4


title: HCC1954--10min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-15


title: HCC1954--10min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-11


title: HCC1954--10min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-10


title: HCC1954--10min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-12


title: HCC1954--5min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-7


title: HCC1954--5min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-5


title: Before infliximab administration, Non-remission-6


title: HCC1954--5min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-4


title: HCC1954--5min-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-3


title: HCC1954--5min-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-2


title: HCC1954--5min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-9


title: HCC1954--5min-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-1


title: HCC60--48hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-45


title: SID 2.5µM 24hr 1

city: Stirling

first: Kevin

last: Petrie

state: Stirling

address: Stirling

department: Biological and Environmental Sciences

country: United Kingdom

institute: University of Stirling

hyb_protocol: The quality and quantity of labeled cRNA was checked and 750 ng of labeled cRNA were hybridized to a GeneChip Human Gene 1.0 ST Arrays using GeneChip Hybridization, Wash, and Stain Kit (Affymetrix).

scan_protocol: The arrays were scanned on a GeneChip Scanner 3000 7G.

data_processing: Raw data were processed with the Expression Console (Affymetrix) for background correction and normalization using RMA algorithm. Secondary analysis of the data was performed by Transcriptome analysis console (Affymetrix).

source_name: 234 breast cancer cell line

label_protocol: All samples were normalized to 200ng and processed according to standard Affymetrix protocols using GeneChip WT Terminal Labeling and Controls Kit (Affymetrix) and WT Expression Kit (Ambion).

growth_protocol: MDA-MB-231 cell lines were maintained in DMEM supplemented with 10% FBS, 1% GlutaMAX (Invitrogen), 10mM HEPES, 1mM sodium pyruvate, non-essential amino acids and 1% antibiotic-antimycotic solution.

extract_protocol: Total RNA was isolated using the ZR RNA MiniPrep Kit (Zymo Research). The concentration and quality of the total RNA was assessed on an Agilent 2100 BioAnalyzer (Agilent Technologies).

treatment_protocol: MDA-MB-231 cells were treated with scrambled SID control, 2.5µM SID peptide or untreated for 24h.

tag: cell type

value: Breast cancer cell line


title: Scr SID 3

source_name: 233 breast cancer cell line


title: HCC60--48hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-44


title: HCC1954--5min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-8


title: Untreated 3 (2)


title: SID 2.5µM 24hr 3

source_name: 236 breast cancer cell line


title: Before infliximab administration, Non-remission-1


title: Untreated 2 (2)

source_name: 232 breast cancer cell line


title: HCC60--48hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-43


title: Before infliximab administration, Non-remission-2


title: HCC1954--5min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC1954-6


title: Untreated 1 (2)

source_name: 231 breast cancer cell line


title: Scr SID 1


title: Heart_13weeks_NT_rep7

city: Calgary

first: Andreas

last: Brodehl

state: Alberta

address: 3280 Hospital Drive NW

department: Libin Institute for Cardiovascular Research

country: Canada

institute: University of Calgary

description: C57 BDF1, heart, 13 weeks

hyb_protocol: Following fragmentation, 2.75 ug ssDNA were hybridized to Affymetrix GeneChip Mouse 1.0ST Array at 45°C for 16h.

scan_protocol: GeneChips were scanned using Affymetrix GeneChip Scanner 3000 7G System.

data_processing: Array data files were generated with GeneChip® Command Console® Software (AGCC). Normalization and statistical significant analysis was carried out using the RMA package in Bioconductor.  Twelve additional background arrays processed at the same facility were used in the normalization step. The Fold Change between treatment and control was based on the p<0.05 from unpaired T-test (Asymptotic and Benjamini Hochberg FDR).

organism: Mus musculus

source_name: heart, non transgenic, 13 weeks

label_protocol: 250 ng of RNA of each sample with RIN higher than 9 was labelled with Whole Transcript plus kit (Affymetrix)

growth_protocol: DSC2 transgenic mice and control mice were analyzed at the age of 3.5 and 13 weeeks.

extract_protocol: Frozen hearts were pulverized under liquid N2. Afterwards, RNA was isolated with the PerfectaPure RNA fibrous tissue kit (5PRIME, Hilden, Germany) and concentrations were determined by using the NanoVue Plus spectrophotometer (GE Healthcare, Buckinghamshire, UK).

treatment_protocol: Mice were anesthetized by using isoflurane and euthanized by cervical dislocation. Subsequently, hearts were dissected, washed with PBS and immediately frozen in liquid N2 and stored at  80°C.

value: C57 BDF1

value: heart

value: 13 weeks


title: Scr SID 2


title: HCC60--48hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-42


title: Heart_13weeks_DSC2_rep5

description: C57 BDF1-Tg(alphaMHC-DSC2), heart, 13 weeks

value: C57 BDF1-Tg(alphaMHC-DSC2)


title: Heart_13weeks_DSC2_rep6


title: Heart_13weeks_NT_rep5

description: C57 BDF1, heart, 13 weeks


title: Heart_13weeks_DSC2_rep7


title: Heart_13weeks_NT_rep4

description: C57 BDF1, heart, 13 weeks

source_name: heart, DSC2-transgenic, 13 weeks


title: HCC60--48hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-40


title: HCC60--48hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-38


title: SID 2.5µM 24hr 2

source_name: 235 breast cancer cell line


title: HCC60--48hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-37


title: HCC60--24hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-35


title: HCC60--24hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-34


title: HCC60--24hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-33


title: Heart_13weeks_NT_rep6

description: C57 BDF1, heart, 13 weeks


title: Heart_3.5weeks_DSC2_rep6

description: C57 BDF1-Tg(alphaMHC-DSC2), heart, 3.5 weeks

value: 3.5 weeks


title: HCC60--48hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-41


title: Heart_13weeks_DSC2_rep4


title: HCC60--24hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-31


title: Heart_3.5weeks_NT_rep7

description: C57 BDF1, heart, 3.5 weeks


title: Heart_3.5weeks_DSC2_rep5


title: HCC60--48hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-39


title: Heart_3.5weeks_DSC2_rep4


title: HCC60--24hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-30


title: Heart_3.5weeks_NT_rep4

description: C57 BDF1, heart, 3.5 weeks

source_name: heart, non transgenic, 3.5 weeks


title: HCC60--24hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-32


title: Heart_3.5weeks_NT_rep6

description: C57 BDF1, heart, 3.5 weeks


title: Heart_3.5weeks_DSC2_rep7


title: Heart_3.5weeks_NT_rep3

description: C57 BDF1, heart, 3.5 weeks


title: Heart_3.5weeks_DSC2_rep3


title: Heart_3.5weeks_NT_rep5

description: C57 BDF1, heart, 3.5 weeks


title: Heart_3.5weeks_NT_rep2

description: C57 BDF1, heart, 3.5 weeks


title: HCC60--24hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-29