title: Heart_3.5weeks_NT_rep1

description: C57 BDF1, heart, 3.5 weeks


title: Heart_3.5weeks_DSC2_rep2

status: Public on Jul 21 2016

type: RNA

city: Calgary

first: Andreas

last: Brodehl

state: Alberta

address: 3280 Hospital Drive NW

department: Libin Institute for Cardiovascular Research

country: Canada

institute: University of Calgary

description: C57 BDF1-Tg(alphaMHC-DSC2), heart, 3.5 weeks

hyb_protocol: Following fragmentation, 2.75 ug ssDNA were hybridized to Affymetrix GeneChip Mouse 1.0ST Array at 45°C for 16h.

scan_protocol: GeneChips were scanned using Affymetrix GeneChip Scanner 3000 7G System.

data_processing: Array data files were generated with GeneChip® Command Console® Software (AGCC). Normalization and statistical significant analysis was carried out using the RMA package in Bioconductor.  Twelve additional background arrays processed at the same facility were used in the normalization step. The Fold Change between treatment and control was based on the p<0.05 from unpaired T-test (Asymptotic and Benjamini Hochberg FDR).

label: biotin

molecule: total RNA

organism: Mus musculus

source_name: heart, non transgenic, 3.5 weeks

label_protocol: 250 ng of RNA of each sample with RIN higher than 9 was labelled with Whole Transcript plus kit (Affymetrix)

growth_protocol: DSC2 transgenic mice and control mice were analyzed at the age of 3.5 and 13 weeeks.

extract_protocol: Frozen hearts were pulverized under liquid N2. Afterwards, RNA was isolated with the PerfectaPure RNA fibrous tissue kit (5PRIME, Hilden, Germany) and concentrations were determined by using the NanoVue Plus spectrophotometer (GE Healthcare, Buckinghamshire, UK).

treatment_protocol: Mice were anesthetized by using isoflurane and euthanized by cervical dislocation. Subsequently, hearts were dissected, washed with PBS and immediately frozen in liquid N2 and stored at  80°C.

tag: strain

value: C57 BDF1-Tg(alphaMHC-DSC2)

tag: tissue

value: heart

tag: age

value: 3.5 weeks


title: HCC60--24hr-SFM--rep1

type: protein

city: Pittsburgh

first: Alison

middle: M

last: Nagle

state: PENNSYLVANIA

address: 204 Craft Ave

department: Pharmacology and Chemical Biology

country: USA

institute: University of Pittsburgh

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-28

hyb_protocol: The diluted lysates were arrayed on nitrocellulose-coated FAST slides. Each slide was probed with a primary antibody and a biotin-conjugated secondary antibody.

scan_protocol: Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction. Slides were scanned on a flatbed scanner to produce 16-bit tiff image. Spots from tiff images were identified and the density was quantified by MicroVigene.

data_processing:
Relative protein levels for each sample were determined by interpolation of each dilution curves from the standard curve (supercurve) of the slide (antibody).
Supercurve is constructed by a script in R written by Bioinformatics.  These values (given as log2 values) are defined as Supercurve Log 2 value  (format reported in the Metadata). All the data points were normalized for protein loading and transformed to linear value designated as Normalized Linear.

molecule: protein

organism: Homo sapiens

source_name: breast cancer

growth_protocol: The cell lines were cultured in DMEM, DMEM/F-12, RPMI, L15 or McCoys 5A media supplemented with 10% FBS or horse serum according to the conditions provided by Neve et al. (2006).

extract_protocol: Cell lysates were harvested by washing in ice-cold PBS twice, and lysing immediately in RPPA lysis buffer with protease inhibitor (1% Triton X-100, 50mM Hepes, 150mM NaCl, 1.5mM MgCl2, 1mM EGTA, 100mM NaF, 10mM NaPPi, 10% Glycerol, 1mM Na3VO4, 1mM PMSF, 10g/ml Aprotinin). Lysates were sonicated and centrifuged at 14,000 rpm at 4oC for 10 min. Protein concentrations were determined by BCA. Then, lysates were mixed with 4X SDS/2-ME sample buffer to have final concentration of 1μg/ml. The lysates were boiled for 5 min and diluted two-fold five times.

treatment_protocol: Cells were plated in triplicate 6cm dishes per treatment time point and then switched to serum-free medium (SFM). Cells were serum starved for 24 hours, then treated with 10nM IGF1 or Ins for 5m, 10m, 30m, 6h, 24h, and 48h.

tag: cell line


title: Heart_3.5weeks_DSC2_rep1

source_name: heart, DSC2-transgenic, 3.5 weeks


title: HCC60--6hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-26


title: HCC60--6hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-25


title: Heart_13weeks_NT_rep3

description: C57 BDF1, heart, 13 weeks

value: C57 BDF1

value: 13 weeks


title: Heart_13weeks_NT_rep2

description: C57 BDF1, heart, 13 weeks


title: HCC60--6hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-22


title: HCC60--6hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-23


title: HCC60--6hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-24


title: HCC60--30min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-20


title: HCC60--30min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-21


title: HCC60--30min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-18


title: HCC60--30min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-17


title: HCC60--6hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-27


title: HCC60--30min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-16


title: HCC60--10min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-15


title: HCC60--10min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-14


title: Heart_13weeks_DSC2_rep3

description: C57 BDF1-Tg(alphaMHC-DSC2), heart, 13 weeks


title: Heart_13weeks_DSC2_rep1


title: HCC60--10min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-13


title: Heart_13weeks_NT_rep1

description: C57 BDF1, heart, 13 weeks


title: HCC60--30min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-19


title: Heart_13weeks_DSC2_rep2


title: HCC60--10min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-12


title: HCC60--10min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-11


title: BMMs control, biological rep2

city: Chengdu

first: Quan

last: Yuan

state: Sichuan

address: 14 Third section, Renmin Nan Road

country: China

institute: West China Hospital of Stomatology, Sichuan University

description: Gene expression data from mosue BMMs

hyb_protocol: 5.15 ug sscDNA was biotin end-labeled with TdT enzyme for 17 hr at 45 °C at 60 rpm in an Affymetrix Hybridization Oven, followed by wash stain protocol FS450_007 on an  Affymetrix Fluidics Station FS450.

scan_protocol: Mouse Gene ST Arrays were scanned with an Affymetrix 3000 7G Gene Chip Scanner.

data_processing: Affymetrix Express Console (build 1.4.1.46) and Transcriptome Analysis Console 3.0

source_name: mouse BMMs

label_protocol: 150 ng total RNA input was converted to cDNA,and in vitro transcribed and amplified over night, followed by bead purification, 10 ug IVT cRNA was converted to ssCDNA with random dUTP incorporation, and bead purified. 5.5 ug sscDNA was enzymatically fragmented to an average size of 75 bp. All steps were performed using the Affymetrix GeneChip® WT PLUS Reagent Kit according to the manufacturer's instructions

growth_protocol: We cultured the cells in α-MEM (Gibco) supplemented with 10% fetal bovine serum (FBS), 100 units/ml penicillin, 100 μg/ml streptomycin and 10 mM HEPES. Tibiae of 9-week-old mice were aseptically removed and the bone marrow cells were flushed out. The cells were suspended and cultured in the medium containing 100 ng/ml M-CSF overnight at 37°C. The untouched cells were collected and further cultured with 100 ng/ml M-CSF for two days to obtain BMMs.

extract_protocol: Total RNA was isolated using an RNeasy kit (Qiagen) according to the manufacturer’s protocol

treatment_protocol: BMMs were cultured in the presence of 100 ng/ml M-CSF and 50 ng/ml RANKL, with or without 100 ng/ml GDF11 for 24 hours

value: bone marrow–derived macrophages (BMMs)

tag: treatment

value: Vehicle control


title: BMMs GDF11 treated, biological rep3

description: Gene expression data from mosue BMMs treated with 100ng/ml rGDF11 for 24 hours

value: 100 ng/ml rGDF11


title: BMMs control, biological rep3

description: Gene expression data from mosue BMMs


title: non-Exercise 331

city: Little Rock

first: Intawat

last: Nookaew

address: 4301 W Markham St

department: Department of Biomedical Informatics

institute: University of Arkansas for Medical Sciences

hyb_protocol: Samples were hybridized with GeneChip Mouse Gene 1.0 ST Arrays (Affymetrix)

scan_protocol: Array scanning was performed according to the manufacturer's instruction (Affymetrix) by A GeneChip Fluidics Station FS-400 and GeneChip Scanner 3000 7G

data_processing: Probe Logarithmic Intensity Error (PLIER) log2 normalize signal

source_name: melanoma tissue

label_protocol: Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).

extract_protocol: RNA was isolated using the RNeasy mini kit or the RNeasy Lipid tissue mini kit (QIAGEN), according to the manufacturer's instructions.

tag: gender

value: Male

value: melanoma tumor

tag: group

value: non-Exercise


title: HCC60--10min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-10


title: BMMs control, biological rep1

description: Gene expression data from mosue BMMs


title: non-Exercise 330


title: HCC60--5min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-9


title: BMMs GDF11 treated, biological rep1


title: HCC60--5min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-8


title: BMMs GDF11 treated, biological rep2


title: HCC60--5min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-7


title: HCC60--24hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-36


title: non-Exercise 326


title: Exercise 310

value: Exercise


title: HCC60--5min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-6


title: non-Exercise 327


title: HCC60--5min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-5


title: HCC60--5min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-4


title: HCC60--5min-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-3


title: non-Exercise 329


title: Exercise 306


title: Exercise 301


title: HCC60--5min-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-2


title: HCC60--5min-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
HCC60-1


title: ES[MC1R:tet Plac1] Dox-/+48 hour Replicate 1

city: Baltimore

first: Minoru

last: Ko

state: MD

address: 251 Bayview Blvd, Suite 100, 10C

department: National Institute on Aging

institute: NIH

description:
Plac1_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

hyb_protocol: Hybridized to Agilent-015087 arrays according to the manufacturer's protocol (Two-Color Microarray-Based Gene Expression Analysis Protocol, Product # G4140-90050, Version 5.0.1, August 2006).

scan_protocol: Slides were scanned with an Agilent DNA Microarray Scanner (model G2505-64120) at 100% and 10% PMT in both channels, with a scan resolution of 5um.

data_processing: Let x-norm = x - y + mean(y), where x = log10(Cy3), y =log10(Cy5). x-batch = x - median(new data) + median(old data) for every gene. The batched normalized value x-batch is used for the final processed VALUE.

source_name: Plac1 Dox- for 48 hours

label_protocol: Total RNA was labeled using the Agilent Low RNA Input Fluorescent Linear Amplification kit according to the manufacturer's instructions.

growth_protocol: Samples are cultured for 48 hours under Dox- (Cy3) and Dox+ (Cy5) conditions. Cells were plated in regular ES medium with Doxycycline (0.2ug/ml) and Puromycin (1.5ug/ml). Approximately 18 hours after plating, the cells were rinsed once with PBS, then the medium was replaced with warmed medium with Doxycycline. Three hours after the first medium change, the medium was changed again. Finally, at three hours after the second medium change, the medium was replaced for a final time. Thereafter, the medium was changed daily. At 48 hours after the final wash, the cells were harvested in Trizol. There was no medium change on the day of RNA harvest.

extract_protocol: The samples were dissolved in the well with TRIzol reagent 1/2 mL. They were then transferred to Phase Lock Gel Heavy tubes (eppendorf) and 0.2 volumes of chloroform (Sigma-Aldrich) was added to each sample. They were mixed by hand and allowed to stand for 2-5 min at room temperature. The samples were then separated by centrifugation for 5 minutes at 15,300 rpm on an eppendorf centrifuge at 4C. The aqueous phase was transferred to another eppendorf tube, and 0.8 volumes of ice-cold isopropanol was added. They were allowed to incubate for a minimum of 10 minutes at room temperature. If longer, they were placed at 4C. The sample was centrifuged again at the same conditions for 15 minutes. After centrifugation, the liquid was dumped out of the tubes and the pellet was washed with 1 mL of ice-cold 70% ethanol. The tube was spun for 5 minutes, then the ethanol was dumped out. Excess was removed with a pipette and the pellet was allowed to air dry until no liquid remained, but no longer. ddH2O was then added in the appropriate volume, the sample was allowed to dissolve for 5 minutes at 55C and then transferred to -80C for storage.

tag: transcription factor

tag: cell type

value: embryonic stem cells

tag: culture condition

value: Dox- for 48 hours

tag: developmental stage

value: embryo

tag: strain/background

tag: individual identifier

source_name: Plac1 Dox+ for 48 hours

value: Dox+ for 48 hours


title: Cama1--48hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-44


title: ES[MC1R:tet Plac1] Dox-/+48 hour Replicate 2

description:
Plac1_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: Cama1--48hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-45


title: Cama1--48hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-43


title: Cama1--48hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-41


title: Cama1--48hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-42


title: Cama1--48hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-40


title: Cama1--48hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-38


title: Cama1--24hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-36


title: Cama1--24hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-35


title: Exercise 309


title: ES[MC1R:tet Cdkn1a] Dox-/+48 hour Replicate 1

description:
Cdkn1a_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Cdkn1a Dox- for 48 hours

source_name: Cdkn1a Dox+ for 48 hours


title: ES[MC1R:tet Snai3] Dox-/+48 hour Replicate 2

description:
Snai3_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Snai3 Dox- for 48 hours

source_name: Snai3 Dox+ for 48 hours


title: Cama1--48hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-37


title: ES[MC1R:tet Pbx1] Dox-/+48 hour Replicate 2

description:
Pbx1_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Pbx1 Dox- for 48 hours

source_name: Pbx1 Dox+ for 48 hours


title: ES[MC1R:tet Cdkn1a] Dox-/+48 hour Replicate 2

description:
Cdkn1a_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: ES[MC1R:tet Pbx1] Dox-/+48 hour Replicate 1

description:
Pbx1_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: ES[MC1R:tet Zfp281] Dox-/+48 hour Replicate 1

description:
Zfp281_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Zfp281 Dox- for 48 hours

source_name: Zfp281 Dox+ for 48 hours


title: Exercise 300


title: Cama1--24hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-34


title: Cama1--24hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-33


title: ES[MC1R:tet Snai3] Dox-/+48 hour Replicate 1

description:
Snai3_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: Cama1--24hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-32


title: Cama1--24hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-31


title: ES[MC1R:tet Prdm1] Dox-/+48 hour Replicate 2

description:
Prdm1_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Prdm1 Dox- for 48 hours

source_name: Prdm1 Dox+ for 48 hours


title: ES[MC1R:tet Prdm1] Dox-/+48 hour Replicate 1

description:
Prdm1_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: Cama1--48hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-39


title: ES[MC1R:tet Nkx2-3] Dox-/+48 hour Replicate 2

description:
Nkx2-3_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Nkx2-3 Dox- for 48 hours

source_name: Nkx2-3 Dox+ for 48 hours


title: Cama1--24hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-30

source_name: breast cancer


title: ES[MC1R:tet Zfp281] Dox-/+48 hour Replicate 2

description:
Zfp281_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: ES[MC1R:tet Nkx2-3] Dox-/+48 hour Replicate 1

description:
Nkx2-3_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: ES[MC1R:tet Lhx1] Dox-/+48 hour Replicate 2

description:
Lhx1_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Lhx1 Dox- for 48 hours

source_name: Lhx1 Dox+ for 48 hours


title: ES[MC1R:tet Foxh1] Dox-/+48 hour Replicate 2

description:
Foxh1_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Foxh1 Dox- for 48 hours

source_name: Foxh1 Dox+ for 48 hours


title: ES[MC1R:tet Foxh1] Dox-/+48 hour Replicate 1

description:
Foxh1_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: Cama1--24hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-29


title: ES[MC1R:tet Lhx1] Dox-/+48 hour Replicate 1

description:
Lhx1_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: Cama1--24hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-28


title: ES[MC1R:tet St18] Dox-/+48 hour Replicate 1

description:
St18_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: St18 Dox- for 48 hours

source_name: St18 Dox+ for 48 hours


title: Cama1--6hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-27


title: Cama1--6hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-26


title: Cama1--6hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-25


title: ES[MC1R:tet St18] Dox-/+48 hour Replicate 2

description:
St18_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: Cama1--6hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-22


title: Cama1--6hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-23


title: Cama1--30min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-21


title: Cama1--30min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-20


title: Cama1--30min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-18


title: Cama1--30min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-19


title: Cama1--30min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-17


title: ES[MC1R:tet Barhl2] Dox-/+48 hour Replicate 1

description:
Barhl2_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Barhl2 Dox- for 48 hours

source_name: Barhl2 Dox+ for 48 hours


title: ES[MC1R:tet Dlx2] Dox-/+48 hour Replicate 2

description:
Dlx2_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Dlx2 Dox- for 48 hours

source_name: Dlx2 Dox+ for 48 hours


title: ES[MC1R:tet Barhl2] Dox-/+48 hour Replicate 2

description:
Barhl2_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: Cama1--10min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-15


title: Cama1--6hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-24


title: ES[MC1R:tet Nkx6-3] Dox-/+48 hour Replicate 2

description:
Nkx6-3_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Nkx6-3 Dox- for 48 hours

source_name: Nkx6-3 Dox+ for 48 hours


title: ES[MC1R:tet Esrrg] Dox-/+48 hour Replicate 2

description:
Esrrg_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Esrrg Dox- for 48 hours

value: Esrrg

source_name: Esrrg Dox+ for 48 hours


title: Cama1--10min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-14


title: ES[MC1R:tet Lhx8] Dox-/+48 hour Replicate 1

description:
Lhx8_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Lhx8 Dox- for 48 hours

source_name: Lhx8 Dox+ for 48 hours


title: ES[MC1R:tet Lhx8] Dox-/+48 hour Replicate 2

description:
Lhx8_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: Cama1--10min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-13

source_name: breast cancer


title: ES[MC1R:tet Dlx2] Dox-/+48 hour Replicate 1

description:
Dlx2_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: ES[MC1R:tet Etv2] Dox-/+48 hour Replicate 2

description:
Etv2_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Etv2 Dox- for 48 hours

source_name: Etv2 Dox+ for 48 hours


title: ES[MC1R:tet Sry] Dox-/+48 hour Replicate 1

description:
Sry_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Sry Dox- for 48 hours

value: Sry

source_name: Sry Dox+ for 48 hours


title: ES[MC1R:tet Etv2] Dox-/+48 hour Replicate 1

description:
Etv2_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: ES[MC1R:tet Sry] Dox-/+48 hour Replicate 2

description:
Sry_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: ES[MC1R:tet Nkx6-3] Dox-/+48 hour Replicate 1

description:
Nkx6-3_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: ES[MC1R:tet Cphx1] Dox-/+48 hour Replicate 2

description:
Cphx1_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Cphx1 Dox- for 48 hours

source_name: Cphx1 Dox+ for 48 hours


title: ES[MC1R:tet Esrrg] Dox-/+48 hour Replicate 1

description:
Esrrg_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: ES[MC1R:tet Cphx1] Dox-/+48 hour Replicate 1

description:
Cphx1_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: Cama1--10min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-12


title: ES[MC1R:tet Glis2] Dox-/+48 hour Replicate 2

description:
Glis2_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Glis2 Dox- for 48 hours

source_name: Glis2 Dox+ for 48 hours


title: Cama1--30min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-16


title: Cama1--10min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-11


title: ES[MC1R:tet Glis2] Dox-/+48 hour Replicate 1

description:
Glis2_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: Cama1--5min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-9


title: ES[MC1R:tet Sall2] Dox-/+48 hour Replicate 1

description:
Sall2_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Sall2 Dox- for 48 hours

source_name: Sall2 Dox+ for 48 hours


title: Cama1--5min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-8


title: Cama1--5min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-7


title: Cama1--5min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-6


title: ES[MC1R:tet Nr4a2] Dox-/+48 hour Replicate 2

description:
Nr4a2_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Nr4a2 Dox- for 48 hours

source_name: Nr4a2 Dox+ for 48 hours


title: Cama1--5min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-5


title: Cama1--5min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-4


title: ES[MC1R:tet Nr4a2] Dox-/+48 hour Replicate 1

description:
Nr4a2_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: ES[MC1R:tet Hoxb4] Dox-/+48 hour Replicate 1

description:
Hoxb4_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Hoxb4 Dox- for 48 hours

source_name: Hoxb4 Dox+ for 48 hours


title: ES[MC1R:tet Hoxb4] Dox-/+48 hour Replicate 2

description:
Hoxb4_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: Cama1--5min-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-3


title: ES[MC1R:tet Msx3] Dox-/+48 hour Replicate 2

description:
Msx3_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Msx3 Dox- for 48 hours

source_name: Msx3 Dox+ for 48 hours


title: Cama1--5min-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-2

source_name: breast cancer


title: Cama1--10min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-10


title: Cama1--5min-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
Cama 1-1


title: BT474--48hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-44


title: BT474--48hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-42


title: BT474--48hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-45


title: ES[MC1R:tet Sall2] Dox-/+48 hour Replicate 2

description:
Sall2_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: BT474--48hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-43


title: BT474--48hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-41


title: ES[MC1R:tet Msx3] Dox-/+48 hour Replicate 1

description:
Msx3_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: ES[MC1R:tet Pax3] Dox-/+48 hour Replicate 1

description:
Pax3_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Pax3 Dox- for 48 hours

source_name: Pax3 Dox+ for 48 hours


title: ES[MC1R:tet Pax3] Dox-/+48 hour Replicate 2

description:
Pax3_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: ES[MC1R:tet Pitx1] Dox-/+48 hour Replicate 1

description:
Pitx1_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Pitx1 Dox- for 48 hours

source_name: Pitx1 Dox+ for 48 hours


title: ES[MC1R:tet Pitx1] Dox-/+48 hour Replicate 2

description:
Pitx1_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: ES[MC1R:tet Hoxd1] Dox-/+48 hour Replicate 1

description:
Hoxd1_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Hoxd1 Dox- for 48 hours

source_name: Hoxd1 Dox+ for 48 hours


title: ES[MC1R:tet Hoxd1] Dox-/+48 hour Replicate 2

description:
Hoxd1_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: ES[MC1R:tet Tcfap4] Dox-/+48 hour Replicate 2

description:
Tcfap4_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Tcfap4 Dox- for 48 hours

source_name: Tcfap4 Dox+ for 48 hours


title: BT474--48hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-39


title: BT474--48hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-40


title: BT474--48hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-38


title: ES[MC1R:tet Tcfap4] Dox-/+48 hour Replicate 1

description:
Tcfap4_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: BT474--48hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-37


title: ES[MC1R:tet Dmrtc2] Dox-/+48 hour Replicate 1

description:
Dmrtc2_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Dmrtc2 Dox- for 48 hours

source_name: Dmrtc2 Dox+ for 48 hours


title: BT474--24hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-36


title: BT474--24hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-35


title: ES[MC1R:tet Tcf15] Dox-/+48 hour Replicate 2

description:
Tcf15_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Tcf15 Dox- for 48 hours

source_name: Tcf15 Dox+ for 48 hours


title: BT474--24hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-34


title: BT474--24hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-33


title: BT474--24hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-32


title: BT474--24hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-31


title: BT474--24hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-30


title: ES[MC1R:tet Tcf15] Dox-/+48 hour Replicate 1

description:
Tcf15_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: BT474--6hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-27


title: BT474--24hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-28


title: BT474--24hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-29


title: BT474--6hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-26


title: BT474--6hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-25


title: BT474--6hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-24


title: ES[MC1R:tet Lin28] Dox-/+48 hour Replicate 2

description:
Lin28_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Lin28 Dox- for 48 hours

source_name: Lin28 Dox+ for 48 hours


title: ES[MC1R:tet Trim28] Dox-/+48 hour Replicate 1

description:
Trim28_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Trim28 Dox- for 48 hours

source_name: Trim28 Dox+ for 48 hours


title: ES[MC1R:tet Lin28] Dox-/+48 hour Replicate 1

description:
Lin28_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: ES[MC1R:tet Trp53] Dox-/+48 hour Replicate 2

description:
Trp53_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Trp53 Dox- for 48 hours

source_name: Trp53 Dox+ for 48 hours


title: ES[MC1R:tet Trim28] Dox-/+48 hour Replicate 2

description:
Trim28_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: ES[MC1R:tet Trp53] Dox-/+48 hour Replicate 1

description:
Trp53_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: ES[MC1R:tet Stra8] Dox-/+48 hour Replicate 2

description:
Stra8_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Stra8 Dox- for 48 hours

source_name: Stra8 Dox+ for 48 hours


title: BT474--6hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-23

source_name: breast cancer


title: ES[MC1R:tet Pdx1] Dox-/+48 hour Replicate 2

description:
Pdx1_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Pdx1 Dox- for 48 hours

source_name: Pdx1 Dox+ for 48 hours


title: ES[MC1R:tet Dmrtc2] Dox-/+48 hour Replicate 2

description:
Dmrtc2_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: BT474--6hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-22


title: ES[MC1R:tet Myb] Dox-/+48 hour Replicate 2

description:
Myb_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Myb Dox- for 48 hours

value: Myb

source_name: Myb Dox+ for 48 hours


title: ES[MC1R:tet Stra8] Dox-/+48 hour Replicate 1

description:
Stra8_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: ES[MC1R:tet Myb] Dox-/+48 hour Replicate 1

description:
Myb_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: Fe limited root, biological rep3

city: Mannheim

first: Carsten

last: Sticht

address: Theodor-Kutzer-Ufer

department: ZMF

country: Germany

institute: University Heidelberg

description:
fe3
Gene expression data from roots of 7-week-old Arabidopsis plants grown under Fe limitation.

hyb_protocol: Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.

scan_protocol: Affymetrix GeneArray Scanner3000

data_processing: The data were analyzed with a commercial software called JMP Genomics, version 6, from SAS. Gene expression profiling was performed using arrays of aragene10st -type from Affymetrix. A Custom CDF Version 18 with TAIRIDs based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Medianpolish Probeset Summary

organism: Arabidopsis thaliana

source_name: Arabidopsis roots, Fe limitation

label_protocol: Biotinylated cRNA were prepared according to the standard Affymetrix protocol.

growth_protocol: Plants were grown hydroponically for total 7 weeks under short-day conditions (8h light / 16 h dark)

extract_protocol: RNA was extracted using the peqGOLD Total RNA Kit according to the manufacturer´s instruction

treatment_protocol: For sulfur and iron limitation condition, Arabidopsis plants were grown in full nutrient supply for 2 weeks and then exposed to sulfur or to iron limitation for the following 5 weeks. For the potassium limitation, the concentration of total K+ in the growth media was reduced from 3 mM to 0.3 mM in the first two weeks of growth and then the plants were transferred to potassium free media for the following 5 weeks.

tag: ecotype

tag: genotype

value: Wild type

tag: nutrient limitation

value: iron

value: root


title: BT474--30min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-21


title: Fe limited root, biological rep1

description:
fe1
Gene expression data from roots of 7-week-old Arabidopsis plants grown under Fe limitation.


title: ES[MC1R:tet Tbx6] Dox-/+48 hour Replicate 1

description:
Tbx6_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Tbx6 Dox- for 48 hours

source_name: Tbx6 Dox+ for 48 hours


title: ES[MC1R:tet Tbx6] Dox-/+48 hour Replicate 2

description:
Tbx6_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: Fe limited root, biological rep2

description:
fe2
Gene expression data from roots of 7-week-old Arabidopsis plants grown under Fe limitation.


title: Fe limited root, biological rep4

description:
fe4
Gene expression data from roots of 7-week-old Arabidopsis plants grown under Fe limitation.


title: ES[MC1R:tet Msx1] Dox-/+48 hour Replicate 2

description:
Msx1_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Msx1 Dox- for 48 hours

source_name: Msx1 Dox+ for 48 hours


title: BT474--30min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-20


title: ES[MC1R:tet Msx1] Dox-/+48 hour Replicate 1

description:
Msx1_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: ES[MC1R:tet Pdx1] Dox-/+48 hour Replicate 1

description:
Pdx1_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: BT474--30min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-19


title: BT474--30min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-17


title: BT474--30min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-16


title: BT474--30min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-18


title: BT474--10min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-15


title: S limited root, biological rep4

description:
s4
Gene expression data from roots of 7-week-old Arabidopsis plants grown under S limitation.

source_name: Arabidopsis roots, S limitation

value: sulfur


title: S limited root, biological rep3

description:
s3
Gene expression data from roots of 7-week-old Arabidopsis plants grown under S limitation.


title: BT474--10min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-14


title: ES[MC1R:tet E2f1] Dox-/+48 hour Replicate 1

description:
E2f1_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: E2f1 Dox- for 48 hours

source_name: E2f1 Dox+ for 48 hours


title: ES[MC1R:tet Hoxc9] Dox-/+48 hour Replicate 1

description:
Hoxc9_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Hoxc9 Dox- for 48 hours

source_name: Hoxc9 Dox+ for 48 hours


title: ES[MC1R:tet E2f1] Dox-/+48 hour Replicate 2

description:
E2f1_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: ES[MC1R:tet Pitx2] Dox-/+48 hour Replicate 2

description:
Pitx2_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Pitx2 Dox- for 48 hours

source_name: Pitx2 Dox+ for 48 hours


title: ES[MC1R:tet Pitx2] Dox-/+48 hour Replicate 1

description:
Pitx2_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: BT474--10min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-13

source_name: breast cancer


title: BT474--10min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-12


title: K limited root, biological rep4

description:
k4
Gene expression data from roots of 7-week-old Arabidopsis plants grown under K limitation.

source_name: Arabidopsis roots, K limitation

value: potassium


title: S limited root, biological rep1

description:
s1
Gene expression data from roots of 7-week-old Arabidopsis plants grown under S limitation.


title: ES[MC1R:tet Hoxc9] Dox-/+48 hour Replicate 2

description:
Hoxc9_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: S limited root, biological rep2

description:
s2
Gene expression data from roots of 7-week-old Arabidopsis plants grown under S limitation.


title: K limited root, biological rep1

description:
k1
Gene expression data from roots of 7-week-old Arabidopsis plants grown under K limitation.


title: K limited root, biological rep2

description:
k2
Gene expression data from roots of 7-week-old Arabidopsis plants grown under K limitation.


title: K limited root, biological rep3

description:
k3
Gene expression data from roots of 7-week-old Arabidopsis plants grown under K limitation.


title: Control root, biological rep4

description:
control_4
Gene expression data from roots of 7-week-old Arabidopsis plants grown in full nutrient media.

source_name: Arabidopsis roots, control condition

value: control


title: BT474--10min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-11


title: Control root, biological rep3

description:
control_3
Gene expression data from roots of 7-week-old Arabidopsis plants grown in full nutrient media.


title: ES[MC1R:tet Foxn4] Dox-/+48 hour Replicate 1

description:
Foxn4_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Foxn4 Dox- for 48 hours

source_name: Foxn4 Dox+ for 48 hours


title: Control root, biological rep2

description:
control_2
Gene expression data from roots of 7-week-old Arabidopsis plants grown in full nutrient media.


title: ES[MC1R:tet Foxn4] Dox-/+48 hour Replicate 2

description:
Foxn4_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: BT474--10min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-10


title: ES[MC1R:tet Hoxb1] Dox-/+48 hour Replicate 1

description:
Hoxb1_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Hoxb1 Dox- for 48 hours

source_name: Hoxb1 Dox+ for 48 hours


title: BT474--5min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-9


title: BT474--5min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-8


title: BT474--5min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-7


title: S2197F_37C_rep3

city: San Francisco

first: Megan

last: Mayerle

state: California

address: Genetech Hall Room N374, 600 16th St

department: Biochemistry and Biophysics

institute: University of California San Francisco

description:
Sample rep 3 - mutant vs wt
Each array provides data on total mRNA levels, pre-mRNA levels, and mature mRNA levels, as indicated in the associated raw data. For the associated FinalizedDataForPaper file (linked as supplementary file on Series record), each individual array was analyzed with its dye flip in 3 separate biological replicates, which were then averaged:
S2197F2 vs. WT2* = rep1
S2197F3 vs. WT3* = rep2
S2197F4 vs. WT4* = rep3

hyb_protocol: Hybridization occurred in Agilent Hybridization Buffer at 65C for 16 hours

scan_protocol: Microarray fluorescence was measured at 532 and 635 nm with an Axon Instruments GenePix 4000B

data_processing: Resultant images processed with GenePixPro v. 5.1. Ratio values derived from the median pixel intensities for the 635 nm and 532 nm images of each spot were used to represent probe behaviors in data preprocessing analysis. Spot ratio data were log2 transformed and normalized within each array using global loess regression implemented in the Bioconductor marray package. All experiments were performed as dye-flipped pairs using biological replicates.

organism: Saccharomyces cerevisiae

source_name: wild type

label_protocol: 50 ug of total RNA was primed with 5 mg/mL random dN9 primers and reverse transcribed in the presence of amino-allyl UTP to enable later dye labeling. Reactions were left for at least 2 hours at 42°C. RNA was subsequently removed by treatment with NaOH, and cDNA was split into 2 samples, one labeled with Cy3 and the other with Cy5 to enable comparison of dye-flipped samples. Labeling was performed according to manufacturer's directions using the Amersham Cy3 or Cy5 Monoreactive dye pack.

growth_protocol: A single yeast colony was inoculated into YEPD (rich) media and incubated overnight at 30C. In the morning, the resultant culture was diluted to an OD600 = 0.1 and then placed at 37C until it reached an OD600 of approximately 0.7.

extract_protocol: Total RNA was isolated using the hot phenol method. Frozen yeast pellets were thawed and resuspended in 50 mM Sodium Phosphate, 10 mM EDTA, 1% SDS. This was mixed with an equal volume of acid phenol and incubated at 65C for 10 min with frequent vortexing to mix samples. Samples were then extracted twice more with phenol, before a final chloroform wash. Samples were ethanol precipitated and then resuspended in a suitable volume of pure water.

treatment_protocol: At OD600 = 0.7, yeast cells were pelleted, washed with sterile water, and snap frozen and stored at -80C

tag: genotype/variation

value: Prp8 Wild Type

source_name: mutant

value: Prp8 mutant S2197F


title: BT474--5min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-5


title: BT474--5min-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-3


title: BT474--5min-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-2


title: S2197F_37C_rep2

description:
Sample rep 2 - mutant vs wt
Each array provides data on total mRNA levels, pre-mRNA levels, and mature mRNA levels, as indicated in the associated raw data. For the associated FinalizedDataForPaper file (linked as supplementary file on Series record), each individual array was analyzed with its dye flip in 3 separate biological replicates, which were then averaged:
S2197F2 vs. WT2* = rep1
S2197F3 vs. WT3* = rep2
S2197F4 vs. WT4* = rep3


title: BT474--5min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-4


title: S2197F_37C_rep2 dye flip

description:
Sample rep 2 - dye flip
Each array provides data on total mRNA levels, pre-mRNA levels, and mature mRNA levels, as indicated in the associated raw data. For the associated FinalizedDataForPaper file (linked as supplementary file on Series record), each individual array was analyzed with its dye flip in 3 separate biological replicates, which were then averaged:
S2197F2 vs. WT2* = rep1
S2197F3 vs. WT3* = rep2
S2197F4 vs. WT4* = rep3


title: BT20--48hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-45


title: S2197F_37C_rep1 dye flip

description:
Sample rep 1 - dye flip
Each array provides data on total mRNA levels, pre-mRNA levels, and mature mRNA levels, as indicated in the associated raw data. For the associated FinalizedDataForPaper file (linked as supplementary file on Series record), each individual array was analyzed with its dye flip in 3 separate biological replicates, which were then averaged:
S2197F2 vs. WT2* = rep1
S2197F3 vs. WT3* = rep2
S2197F4 vs. WT4* = rep3


title: BT474--5min-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-1


title: BT20--48hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-44


title: BT474--5min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT474-6


title: Control root, biological rep1

description:
control_1
Gene expression data from roots of 7-week-old Arabidopsis plants grown in full nutrient media.


title: ES[MC1R:tet Hey2] Dox-/+48 hour Replicate 1

description:
Hey2_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Hey2 Dox- for 48 hours

source_name: Hey2 Dox+ for 48 hours


title: S2197F_37C_rep3 dye flip

description:
Sample rep 3 - dye flip
Each array provides data on total mRNA levels, pre-mRNA levels, and mature mRNA levels, as indicated in the associated raw data. For the associated FinalizedDataForPaper file (linked as supplementary file on Series record), each individual array was analyzed with its dye flip in 3 separate biological replicates, which were then averaged:
S2197F2 vs. WT2* = rep1
S2197F3 vs. WT3* = rep2
S2197F4 vs. WT4* = rep3


title: ES[MC1R:tet Hoxb1] Dox-/+48 hour Replicate 2

description:
Hoxb1_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: ES[MC1R:tet Dlx4] Dox-/+48 hour Replicate 1

description:
Dlx4_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Dlx4 Dox- for 48 hours

source_name: Dlx4 Dox+ for 48 hours


title: ES[MC1R:tet Hey2] Dox-/+48 hour Replicate 2

description:
Hey2_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: S2197F_37C_rep1

description:
Sample rep 1 - mutant vs wt
Each array provides data on total mRNA levels, pre-mRNA levels, and mature mRNA levels, as indicated in the associated raw data. For the associated FinalizedDataForPaper file (linked as supplementary file on Series record), each individual array was analyzed with its dye flip in 3 separate biological replicates, which were then averaged:
S2197F2 vs. WT2* = rep1
S2197F3 vs. WT3* = rep2
S2197F4 vs. WT4* = rep3


title: ES[MC1R:tet Fezf2] Dox-/+48 hour Replicate 1

description:
Fezf2_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Fezf2 Dox- for 48 hours

source_name: Fezf2 Dox+ for 48 hours


title: ES[MC1R:tet Dlx4] Dox-/+48 hour Replicate 2

description:
Dlx4_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: ES[MC1R:tet Fezf2] Dox-/+48 hour Replicate 2

description:
Fezf2_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: ES[MC1R:tet Pax6] Dox-/+48 hour Replicate 1

description:
Pax6_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Pax6 Dox- for 48 hours

source_name: Pax6 Dox+ for 48 hours


title: ES[MC1R:tet Lmx1a] Dox-/+48 hour Replicate 2

description:
Lmx1a_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Lmx1a Dox- for 48 hours

source_name: Lmx1a Dox+ for 48 hours


title: ES[MC1R:tet Myt1] Dox-/+48 hour Replicate 2

description:
Myt1_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Myt1 Dox- for 48 hours

source_name: Myt1 Dox+ for 48 hours


title: ES[MC1R:tet Lmx1a] Dox-/+48 hour Replicate 1

description:
Lmx1a_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: ES[MC1R:tet Pax6] Dox-/+48 hour Replicate 2

description:
Pax6_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: ES[MC1R:tet Myt1] Dox-/+48 hour Replicate 1

description:
Myt1_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: BT20--48hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-40

source_name: breast cancer


title: BT20--48hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-41


title: BT20--48hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-38


title: BT20--24hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-35


title: BT20--48hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-37


title: BT20--24hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-36


title: BT20--24hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-34


title: ES[MC1R:tet Isl1] Dox-/+48 hour Replicate 2

description:
Isl1_rep2
Biological replicate 2 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.

source_name: Isl1 Dox- for 48 hours

source_name: Isl1 Dox+ for 48 hours


title: BT20--48hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-42


title: BT20--48hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-43


title: BT20--24hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-32


title: ES[MC1R:tet Isl1] Dox-/+48 hour Replicate 1

description:
Isl1_rep1
Biological replicate 1 of 2, Cy3 is Dox- and Cy5 is Dox+ (control) for 48 hours.


title: BT20--24hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-31


title: BT20--24hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-29


title: BT20--24hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-28


title: BT20--48hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-39


title: BT20--6hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-27


title: BT20--6hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-25


title: BT20--6hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-24


title: BT20--6hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-22


title: BT20--30min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-20


title: BT20--6hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-26


title: BT20--30min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-16


title: BT20--30min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-19


title: BT20--30min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-18


title: BT20--30min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-17


title: BT20--10min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-15


title: BT20--10min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-11


title: BT20--10min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-14


title: BT20--24hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-33


title: BT20--10min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-10


title: BT20--10min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-12


title: BT20--10min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-13


title: BT20--5min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-6


title: BT20--5min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-9


title: BT20--24hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-30


title: BT20--5min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-5


title: BT20--5min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-7


title: BT20--30min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-21


title: BT20--6hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-23


title: AU565--48hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
AU565-44


title: BT20--5min-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-3


title: BT20--5min-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-2


title: AU565--48hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
AU565-45


title: AU565--48hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
AU565-40


title: AU565--48hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
AU565-42


title: BT20--5min-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-1


title: AU565--48hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
AU565-43


title: AU565--48hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
AU565-38


title: AU565--24hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
AU565-36


title: AU565--24hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
AU565-35


title: BT20--5min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-8


title: AU565--24hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
AU565-34


title: AU565--24hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
AU565-33


title: AU565--24hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
AU565-32


title: AU565--24hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
AU565-30


title: AU565--24hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
AU565-31


title: AU565--24hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
AU565-29


title: AU565--24hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
AU565-28


title: AU565--48hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
AU565-41


title: AU565--48hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
AU565-37


title: BT20--5min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
BT20-4


title: AU565--6hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
AU565-27


title: AU565--48hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
AU565-39


title: AU565--6hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
AU565-26


title: AU565--6hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
AU565-25


title: AU565--6hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
AU565-23


title: AU565--6hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
AU565-24


title: AU565--6hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
AU565-22


title: AU565--30min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
AU565-21