title: PMN.hd.3

description: PMN cells, from heathy donor PMN.hd.3


title: C147

status: Public on Jul 22 2016

type: RNA

city: Bunkyo-ku

first: Kaoru

last: Mogushi

state: Tokyo

address: 2-1-1 Hongo

department: Intractable Disease Research Center

country: Japan

institute: Juntendo University

description:
Gene expression profile of surgically resected material
C147

hyb_protocol: Hybridization and signal detection of HG-U133 Plus 2.0 arrays (Affymetrix) was performed following the manufacturer's instruction using the Affymetrix Fluidics Station 450.

scan_protocol: GeneChips were scanned using Affymetrix GeneChip Scanner 3000.

data_processing: The data was normalized by robust multichip analysis (RMA) using R/Bioconductor.

label: biotin

molecule: total RNA

organism: Homo sapiens

source_name: patient 147, cancer, LCM

label_protocol: cRNA was prepared using two-cycle target labeling and control reagents kit (Affymetrix, Santa Clara, CA).

extract_protocol: Total RNA was extracted from tissue specimens using RNeasy kit (Qiagen, Hilden, Germany). Integrity of obtained RNA was assessed using Agilent 2100 BioAnalyzer (Agilent Technologies, Palo Alto, CA). All samples had RNA Integrity Number (RIN) greater than 5.0. Contaminant DNA was removed by digestion with RNase-free DNase (Qiagen).

treatment_protocol: Gene expression profiles for 152 cancer tissues from colorectal cancer patients were used.

tag: metastasis

tag: tissue

value: colorectal cancer


title: C146

description:
Gene expression profile of surgically resected material
C146

source_name: patient 146, cancer, LCM

value: distant metastasis


title: C57BL/6_control_4 weeks_rep4

city: Stanford

first: Vivek

last: Bhalla

state: CA

address: 777 Welch Road, Suite DE

country: USA

institute: Stanford University School of Medicine

hyb_protocol: Standard Illumina hybridization protocol

scan_protocol: Standard Illumina scanning protocol

data_processing: The data were normalised using quantile normalisation with IlluminaGUI in R

organism: Mus musculus

source_name: C57BL/6 glomeruli_control_4 weeks

label_protocol: Not provided

growth_protocol: Mouse were acclimated in standard animal housing facility, with free access to normal food and water.

extract_protocol: RNA were isolated from mouse glomeruli by Tri-reagent (Sigma), 1ug total RNA were applied to the microarray.

treatment_protocol: After 1 week acclimation, we injected streptozotocin (or vehicle- sodium citrate, pH 4.5) i.p. in 6 hour fasted mice daily for 5 consecutive days (40mg/kg body weight for DBA/2 mice, and 50mg/kg body weight for C57BL/6 mice). Mice were supplemented with 10% sucrose water for 1 week to prevent hypoglycemia. Four weeks after injection, we sacrificed mice to prepare glomerular total RNA.

tag: strain

tag: diabetic nephropathy susceptability

value: resistant


title: Brain CD11b+ cells [06_03-10-16_S2]

city: NORTH CHICAGO

first: Rahul

last: Vijay

state: IL

address: 3333 GreenBay Road, BSB 2.450

department: Microbiology and Immunology

institute: Rosalind Franklin University

hyb_protocol: RNA for the microarray was processed using a NuGEN WT-Ovation Pico RNA Amplification System together with a NuGEN WT-Ovation Exon Module. Samples were hybridized and loaded onto Affymetrix Mouse gene 2.0. Arrays

scan_protocol: scanned with a HiScan bead array system, and data were collected using GeneChip Operating Software

data_processing: Data from Affymetrix Mouse gene 2.0 Expression BeadChip were normalized and median polished using Robust Multichip Average background correction with log2-adjusted values. After obtaining the log2 expression values for genes, significance testing was performed comparing the two groups (CD11b+ cells from brains of WT and DP1 KO mice). Significance of expression differences was assessed using a false discovery rate (FDR) cutoff of 0.05 and a twofold change. Analysis and visualization of data and pathway analysis were performed using PartekGS software and Ingenuity Pathway Analysis software.

source_name: Naïve mouse brain

label_protocol: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).

extract_protocol: Trizol extraction of total RNA was performed according to the manufacturer's instructions.

value: Brain

tag: genotype

value: WT


title: C57BL/6_control_4 weeks_rep2


title: Brain CD11b+ cells [07_03-10-16_S2]


title: Brain CD11b+ cells [03_03-10-16_S2]

value: DP1 KO


title: C144

description:
Gene expression profile of surgically resected material
C144

source_name: patient 144, cancer, LCM


title: Brain CD11b+ cells [01_03-10-16_S2]


title: PMN.hd.2

city: Philadelphia

first: Priyankara

middle: J

last: Wickramasinghe

state: PA

address: 3601 Spruce Street

department: Bioinformatics

institute: The Wistar Institute

description: PMN cells, from heathy donor PMN.hd.2

hyb_protocol: Hybridized on Illumina Human-HT12 v4 Expression BeadChip Kit using Illumina whole-Genome Gene Exprssion with Intellihyb Seal system which detect signal with streptavidin-Cy3

scan_protocol: Illumina Setting with PMTFactor=2

data_processing: Illumina GenomeStudio software was used to export expression levels and detection p-values for each probe of each sample. Signal intensity data was quantile normalized and  genes that showed insignificant detection p-value (p>0.05) in all samples were removed from further analysis.

source_name: PMN cells from healthy donors

label_protocol: Biotin Labeling of RNA at 400ng with Ambion Illumina® TotalPrep RNA Amplification Kit

growth_protocol: Samples of peripheral blood were collected from patients at Helen F. Graham Cancer Center and University of Pennsylvania.

extract_protocol: total RNA from purified cells was isolated with TRIzol reagent according to the manufacturer’s recommendations

treatment_protocol: PMN-MDSC and PMN were isolated by centrifugation over on double density gradient Histopaque (Sigma) (1.077 to collect PBMC and 1.119 to collect PMN) followed by labeling with CD15-PE mAb (BD Biosciences) and then separated using anti-PE beads and MACS column (Miltenyi). For LOX1+/- isolation, whole blood was enriched for PMNs using MACSxpress® Neutrophil Isolation Kit (Miltenyi) following the protocol provided by the manufacturer.  Cells were then labeled with anti-Lox1-PE mAb (Biolegend) and then separated using anti-PE beads and MACS column (Miltenyi).

tag: selection

value: PMN

tag: cancer

value: heathy donor

tag: subject id


title: C141

description:
Gene expression profile of surgically resected material
C141

source_name: patient 141, cancer, LCM


title: C142

description:
Gene expression profile of surgically resected material
C142

source_name: patient 142, cancer, LCM


title: Brain CD11b+ cells [02_03-10-16_S2]


title: PMN.hd.1

description: PMN cells, from heathy donor PMN.hd.1


title: C57BL/6_control_4 weeks_rep1


title: DBA/2_diabetes_4 weeks_rep4

source_name: DBA/2 glomeruli_diabetes_4 weeks

value: susceptible


title: C143

description:
Gene expression profile of surgically resected material
C143

source_name: patient 143, cancer, LCM


title: DBA/2_diabetes_4 weeks_rep3


title: C57BL/6_control_4 weeks_rep3


title: DBA/2_diabetes_4 weeks_rep2


title: DBA/2_control_4 weeks_rep3

source_name: DBA/2 glomeruli_control_4 weeks


title: C135

description:
Gene expression profile of surgically resected material
C135

source_name: patient 135, cancer, LCM


title: DBA/2_diabetes_4 weeks_rep1


title: C140

description:
Gene expression profile of surgically resected material
C140

source_name: patient 140, cancer, LCM


title: S1_48h_IFN_none

city: York

first: Matthew

middle: Anthony

last: Care

state: North Yorkshire

address: Wentworth Way

department: Department of Biology

country: United Kingdom

institute: The University of York

hyb_protocol: Standard Illumina protocol

scan_protocol: Arrays were scanned on a BeadArray reader

data_processing: Data were quantile normalised using Lumi for R.

label: Biotin

source_name: MACS purified (negative selection) Total B cells from healthy donors.  Day 6 plasmablasts were reseeded. Conditions available in growth protocol.

label_protocol: Standard Illumina protocol

growth_protocol: MACS purified (negative selection) Total B cells from healthy donors.  Day 6 plasmablasts were reseeded at 1 x 10^6 in transwells in media supplemented with IL-6 (10 ng/ml), IL-21 (50 ng/ml), HybridoMax hybridoma growth supplement (11 ul/ml), Lipid Mixture 1, chemically defined (5 ul/ml) and MEM amino acid solution (20 ul/ml) on gamma irradiated M2-10B4 cells (4 x 104/well).

extract_protocol: RNA was extracted with Trizol and amplified using Illumina® TotalPrepTM-96 RNA Amplification Kit

value: Peripheral blood

tag: cell type

value: B cells

tag: gender

value: Male


title: S2_96h_IFN_none


title: C139

description:
Gene expression profile of surgically resected material
C139

source_name: patient 139, cancer, LCM


title: C136

description:
Gene expression profile of surgically resected material
C136

source_name: patient 136, cancer, LCM


title: S3_48h_IFN_none

value: Female


title: C138

description:
Gene expression profile of surgically resected material
C138

source_name: patient 138, cancer, LCM


title: S1_96h_IFN_none


title: S2_48h_IFN_none


title: C133

description:
Gene expression profile of surgically resected material
C133

source_name: patient 133, cancer, LCM


title: S3_96h_IFN_none


title: DBA/2_control_4 weeks_rep4


title: DBA/2_control_4 weeks_rep2


title: S3_24h_IFN_none


title: S2_24h_IFN_none


title: C130

description:
Gene expression profile of surgically resected material
C130

source_name: patient 130, cancer, LCM


title: DBA/2_control_4 weeks_rep1


title: C129

description:
Gene expression profile of surgically resected material
C129

source_name: patient 129, cancer, LCM


title: S2_12h_IFN_none


title: S3_12h_IFN_none


title: C12

description:
Gene expression profile of surgically resected material
C12

source_name: patient 012, cancer, LCM


title: S1_24h_IFN_none


title: C127

description:
Gene expression profile of surgically resected material
C127

source_name: patient 127, cancer, LCM


title: C125

description:
Gene expression profile of surgically resected material
C125

source_name: patient 125, cancer, LCM


title: C117

description:
Gene expression profile of surgically resected material
C117

source_name: patient 117, cancer, LCM


title: S2_6h_IFN_none


title: S1_12h_IFN_none


title: S1_6h_IFN_none


title: C119

description:
Gene expression profile of surgically resected material
C119

source_name: patient 119, cancer, LCM


title: S3_1h_IFN_none


title: Control rep4

first: AKINORI

last: KASHIO

address: 7-3-1 Hongo

department: Otolaryngology

institute: The University of Tokyo

description: Gene expression data treated with normal chow

hyb_protocol: Hybridization (Affymetrix Hybridization oven 640), washing, and staining (Affymetrix Fluidics station 400) were accomplished according to the manufacture’s instructions.

scan_protocol: Arrays were scanned on the Affymetrix GeneChip Scanner 3000. Image analysis was performed using the Affymetrix software.

data_processing: All arrays were scaled to 1500 to correct for differences in overall signal before analysis in GeneChip® Operating Software (GCOS) 1.3 (Affymetrix). Further analysis and comparisons between experiments was also done in GCOS 1.3 (Affymetrix) Software.

source_name: Mouse cochlea treated with normal chow

label_protocol: cDNA synthesis from total RNA (Invitrogen), cRNA amplification and labeling (Enzo BioArray IVT kit) were accomplished according to the manufacture’s instructions (Affymetrix).

growth_protocol: All mice were raised under 12:12 dark/light conditions, and housed in the the University of Tokyo-approved Animal Care Facility, and were provided a nonpurified diet and acidified water ad libitum.

extract_protocol: Total RNA was isolated from cochlea tissues using the TriZol reagent (Invitrogen) according to the manufacture’s instructions.

treatment_protocol: To identify genes associated with GeO2 application for 4 months in diet, each 6 month sample (n = 5) was compared to each 6 months old sample (n = 5) teated with normal chow, generating a total of 25 pairwise comparisons.

value: CBA

tag: age

value: 6 month old

tag: agent

value: control

value: cochlea


title: Control rep3


title: C123

description:
Gene expression profile of surgically resected material
C123

source_name: patient 123, cancer, LCM

value: colorectal cancer


title: C124

description:
Gene expression profile of surgically resected material
C124

source_name: patient 124, cancer, LCM


title: C116

description:
Gene expression profile of surgically resected material
C116

source_name: patient 116, cancer, LCM


title: S3_3h_IFN_none


title: S1_1h_IFN_none


title: S2_1h_IFN_none


title: S3_6h_IFN_none


title: Control rep5


title: C115

description:
Gene expression profile of surgically resected material
C115

source_name: patient 115, cancer, LCM


title: Control rep1


title: C110

description:
Gene expression profile of surgically resected material
C110

source_name: patient 110, cancer, LCM


title: S1_96h_IFN_gamma

source_name: MACS purified (negative selection) Total B cells from healthy donors.  Day 6 plasmablasts were reseeded. Conditions available in growth protocol.

growth_protocol: MACS purified (negative selection) Total B cells from healthy donors.  Day 6 plasmablasts were reseeded at 1 x 10^6 in transwells in media supplemented with IL-6 (10 ng/ml), IL-21 (50 ng/ml), HybridoMax hybridoma growth supplement (11 ul/ml), Lipid Mixture 1, chemically defined (5 ul/ml) and MEM amino acid solution (20 ul/ml) on gamma irradiated M2-10B4 cells (4 x 104/well) with IFN-γ (200 IU/ml).


title: S2_96h_IFN_gamma


title: S2_3h_IFN_none


title: C108

description:
Gene expression profile of surgically resected material
C108

source_name: patient 108, cancer, LCM


title: Control rep2


title: C103

description:
Gene expression profile of surgically resected material
C103

source_name: patient 103, cancer, LCM


title: C109

description:
Gene expression profile of surgically resected material
C109

source_name: patient 109, cancer, LCM


title: C102

description:
Gene expression profile of surgically resected material
C102

source_name: patient 102, cancer, LCM


title: S3_48h_IFN_gamma


title: C08

description:
Gene expression profile of surgically resected material
C08

source_name: patient 008, cancer, LCM


title: S3_96h_IFN_gamma


title: Germanium rep5

description: Gene expression data treated with 0.15% GeO2

source_name: Mouse cochlea treated with germanium


title: S3_12h_IFN_gamma


title: C101

description:
Gene expression profile of surgically resected material
C101

source_name: patient 101, cancer, LCM


title: Germanium rep3


title: Germanium rep1


title: Germanium rep4


title: S2_24h_IFN_gamma


title: S1_48h_IFN_gamma


title: S2_12h_IFN_gamma


title: Germanium rep2


title: S2_3h_IFN_gamma


title: S1_24h_IFN_gamma


title: S3_1h_IFN_gamma


title: S2_48h_IFN_gamma


title: S3_24h_IFN_gamma


title: S1_6h_IFN_gamma


title: C06

description:
Gene expression profile of surgically resected material
C06

source_name: patient 006, cancer, LCM

value: distant metastasis


title: S3_6h_IFN_gamma


title: S2_1h_IFN_gamma


title: S3_3h_IFN_gamma


title: S3_96h_IFN_alpha

growth_protocol: MACS purified (negative selection) Total B cells from healthy donors.  Day 6 plasmablasts were reseeded at 1 x 10^6 in transwells in media supplemented with IL-6 (10 ng/ml), IL-21 (50 ng/ml), HybridoMax hybridoma growth supplement (11 ul/ml), Lipid Mixture 1, chemically defined (5 ul/ml) and MEM amino acid solution (20 ul/ml) on gamma irradiated M2-10B4 cells (4 x 104/well) with IFN-α (100 IU/ml).


title: S2_6h_IFN_gamma


title: S1_12h_IFN_gamma


title: S2_48h_IFN_alpha


title: S3_24h_IFN_alpha


title: S1_1h_IFN_gamma


title: S3_48h_IFN_alpha


title: S1_96h_IFN_alpha


title: S3_3h_IFN_alpha


title: S3_6h_IFN_alpha


title: S2_12h_IFN_alpha


title: S1_6h_IFN_alpha


title: S3_1h_IFN_alpha


title: S1_24h_IFN_alpha


title: S3_0h_preTreatment

source_name: MACS purified (negative selection) Total B cells from healthy donors.  Day6 plasmablasts.


title: S2_6h_IFN_alpha


title: S1_12h_IFN_alpha


title: S1_48h_IFN_alpha


title: S2_1h_IFN_alpha


title: S2_0h_preTreatment

growth_protocol: MACS purified (negative selection) Total B cells from healthy donors.  Day6 plasmablasts.


title: S2_3h_IFN_alpha


title: S1_1h_IFN_alpha


title: S2_96h_IFN_alpha


title: S3_12h_IFN_alpha


title: S2_24h_IFN_alpha


title: S1_0h_preTreatment


title: Day1 siG9a2

status: Public on Jul 21 2016

city: Singapore

first: Vinay

middle: Kumar

last: Rao

address: 2 Medical drive

department: Physiology

country: Singapore

institute: NUS

description: Day1 siG9a2

hyb_protocol: Standard Illumina hybridizing protocol

data_processing: Data were normalized using quantile normalization

label_protocol: Standard Illumina labelling protocol

extract_protocol: RNA was isolated from cells and RNA clean up was performed from Qiagen kit. Quality control was performed using Agilent bioanalyzer

value: myoblasts

value: skeletal muscle


title: Day0 siG9a2

description: Day0 siG9a2


title: Day1 siRNA2

description: Day1 siRNA2


title: Day0 siRNA2

description: Day0 siRNA2


title: Cancer-associated fibroblasts_A

city: Lund

first: Björn

last: Nilsson

address: BMC

department: Hematology and Transfusion Medicine

country: Sweden

institute: Lund University

description: Gene expression data from fibrobast activated with Granulin

hyb_protocol: Following fragmentation, human HG U133v2 oligonucleotide microarrays (Affymetrix) were hybridized for 16 h at 45C, stained  and washed according to the Affymetrix protocol.

scan_protocol: Affymetrix GCS 3000 7G scanner using Affymetrix standard protocol

data_processing: Signal normalization was performed by RMA method. RMA expression measure was computed using Bioconductor Affy package in R language (Bioconductor 2.5; R version 2.11.0)

source_name: fibroblast

label_protocol: Biotinylated cRNA were prepared according to the standard Affymetrix protocol.

growth_protocol: Normal human mamary fibroblasts were cultured with DMEM medium containing 10% calf serum and 10% fetal bovine serum

extract_protocol: Total RNA was extracted from fibroblasts using RNA extraction kits according to the manufacturer’s instructions (Qiagen)

treatment_protocol: Cells were cultured for 6 days in presence of 1ug/ml recombinant human granulin protein or PBS control

tag: clinical status

value: Cancer-associated fibroblasts


title: Cancer-associated fibroblasts_B


title: Day0 siRNA1

description: Day0 siRNA1


title: Ascl1_d5_Dox+_rep1

city: Baltimore

first: Minoru

last: Ko

state: MD

address: 251 Bayview Blvd, Suite 100, 10C

department: National Institute on Aging

institute: NIH

hyb_protocol: Not provided

scan_protocol: Slides were scanned with an Agilent DNA Microarray Scanner (model G2565BA), with a scan resolution of 3um.

data_processing:
Data are extracted with Agilent Feature Extraction Software.
The arrays are normalized using quantile method
The data were further processed with NIA ExAtlas utilities. See http://lgsun.grc.nia.nih.gov/exatlas for details.

label_protocol: Total RNA was labeled using the Agilent G4170-90011 miRNA Protocol 3.0 kit according to the manufacturer's instructions.

growth_protocol: For neural differentiation, cells were cultured 3 days in alpha-MEM medium and then - in NeuroCult neural differentiation medium for 2-11 days (total up to 14 days). Doxycycline (Dox) was used to repress the expression of transgenic Ascl1, which was expressed in Dox- conditions and repressed in Dox+ conditions.

extract_protocol: RNA was extracted with mirVana kit (Thermo Fisher Scientific).

value: embryonic stem cells

tag: development stage

value: embryo

tag: strain identifier

tag: individual identifier


title: Day1 siG9a1

description: Day1 siG9a1


title: Day0 siG9a1

description: Day0 siG9a1


title: Ascl1_d7_Dox-_rep1


title: Ascl1_d7_Dox+_rep1


title: Ascl1_d10_Dox+_rep1


title: Ascl1_d5_Dox-_rep1


title: Cancer-associated fibroblasts_C


title: Normal mammary human_A

description: Gene expression data from fibrobast activated with PBS

value: Normal mammary human


title: Day1 siRNA1

description: Day1 siRNA1


title: Ascl1_d10_Dox-_rep1


title: Ascl1_d14_Dox-_rep1


title: Ascl1_d14_Dox+_rep1


title: Normal mammary human_C


title: Granulin-stimulated fibroblasts_B

value: Granulin-stimulated fibroblasts


title: Granulin-stimulated fibroblasts_C


title: Ascl1_d3_Dox+_rep1


title: Ascl1_d3_Dox-_rep1


title: MC1_d3_Dox+_rep1


title: Normal mammary human_B


title: Ascl1_d14_Dox-_mirVANA_rep1

description: Cy5 is mouse reference

scan_protocol: Slides were scanned with an Agilent DNA Microarray Scanner (model G2505-64120) at 100% and 10% PMT in both channels, with a scan resolution of 5um.

data_processing:
Data are extracted with Agilent Feature Extraction Software. The data were further processed with NIA ANOVA tool utilities. See http://lgsun.grc.nia.nih.gov/ANOVA for details.
The data is normalized with the following method:
(1) Take values from the columns gDyeNormSignal, rDyeNormSignal in the raw files and log-transform them using: log10(max(x,10)). These values will be referenced below as Xgi and Xri, where i is array number.
(2) Take average of Xri's for the oligo among 26 arrays in the series: AverXr = average(Xri).
(3) For each array, estimate Yi = Xgi-Xri+AverXr (adjust to UMR).
The result Yi is used as the normalized VALUE. More detailed information with error correction can be found at: http://lgsun.grc.nia.nih.gov/ANOVA/help.html#arrayjoin

label_protocol: Total RNA was labeled using the Agilent Low RNA Input Fluorescent Linear Amplification kit according to the manufacturer's instructions.

growth_protocol: For neural differentiation, cells were cultured 3 days in alpha-MEM medium and then in NeuroCult neural differentiation medium. Cells marked as PSANCAM+ were enriched by magnetic microbeads with anty-PSA-NCAM antibody (Miltenyi Biotec) on day 6 of culturing, whereas remaining cells are marked as PSANCAM-. Both PSANCAM+ and PSANCAM- cells were then cultured for another 8 days (total 14 days in differentiation). A time course experiment with Ascl1 induction did not include cell enrichment procedure. Doxycycline (Dox) was used to repress the expression of transgenic Ascl1, which was expressed in Dox- conditions and repressed in Dox+ conditions.

extract_protocol: RNA was extracted with either Trizol (invitrogen) or mirVana kit (Thermo Fisher Scientific).

source_name: Universal Mouse Reference

extract_protocol: Total RNA was extracted from cultured cells using a Stratagene Absolutely RNA kit. DNase-treated total RNA samples from 11 cultured cell lines derived from embryo, embryo fibroblast, kidney, liver/hepatocyte, lung/alveolar macrophage, B-lymphocyte, T-lymphocyte (thymus), mammary gland, muscle myoblast, skin, and testis were pooled in equal parts. The resulting UMR RNA was mixed with 129ES cell RNA (Lif+) at 2:1 ratio and 2.5ug of mixed RNA was used for labeling in each tube

tag: sample type

value: Reference


title: Granulin-stimulated fibroblasts_A


title: rtr1 H3K36me3 antibody replicate 2

type: genomic

city: Kansas City

first: Madelaine

last: Gogol

state: MO

address: 1000 E. 50th Street

department: Computational Biology Core

institute: Stowers Institute

hyb_protocol: A mass of 5ug cy labeled samples with specific activity of greater than 15 pmol/ug were combined for each channel and hybridized according to the “Agilent Yeast ChIP-on-chip Analysis protocol Version 9.2, Mat 2007”.  Hybridization occurred in an Agilent Rotisserie Hybridization Oven set to 20RPM at 65C for 40 hours.

scan_protocol: Scanning was performed on the Agilent DNA Microarray Scanner (Agilent Technologies, Model#G2505B)

data_processing: Agilent Feature Extraction Software (v 10.5.1.1) was used to quantify images. Data was read into R and normalized using median normalization.

molecule: genomic DNA

organism: Saccharomyces cerevisiae

source_name: yeast, rtr1, H3K36me3 ip, rep2

label_protocol: Input was labeled with Cy3 dye, while immunoprecipitated samples were labeled with Cy5 dye.

growth_protocol: Cells were grown to an OD600 of 0.8-1 in YPD at 30 degrees.

extract_protocol: Immune complexes were eluted at 65C for 30min with 1% SDS in TE supplemented with 250mM NaCl.  Samples were treated with RNaseA followed by Proteinase K and crosslinks were reversed overnight at 65C.  DNA was recovered by phenol chloroform isoamyl alcohol extraction followed by ethanol precipitation.

treatment_protocol: Immunoprecipitation was performed with 5 uL of antibody

tag: ip antibody

value: H3K36me3 antisera (5uL)

source_name: yeast, rtr1, H3K36me3 input, rep2

value: none (input)


title: Ascl1_d14_Dox+_mirVANA_rep1

description: Cy5 is mouse reference


title: Ascl1_d10_Dox-_mirVANA_rep1

description: Cy5 is mouse reference


title: Ascl1_d14_Dox+_PSANCAM-_mirVANA_rep1

description: Biological replicate 1 of 2, Cy3 is mouse RNA and Cy5 is mouse reference


title: Ascl1_d5_Dox-_mirVANA_rep1

description: Cy5 is mouse reference


title: Ascl1_d14_Dox+_PSANCAM-_mirVANA_rep2

description: Biological replicate 2 of 2, Cy3 is mouse RNA and Cy5 is mouse reference


title: Ascl1_d5_Dox+_mirVANA_rep1

description: Cy5 is mouse reference


title: Ascl1_d3_Dox+_mirVANA_rep1

description: Cy5 is mouse reference


title: rtr1 H3K36me3 antibody replicate 1

source_name: yeast, rtr1, H3K36me3 ip, rep1

source_name: yeast, rtr1, H3K36me3 input, rep1


title: WT H3K36me3 antibody replicate 1

source_name: yeast, wt, H3K36me3 ip, rep1

value: wt

source_name: yeast, wt, H3K36me3 input, rep1


title: Ascl1_d3_Dox-_mirVANA_rep1

description: Cy5 is mouse reference


title: Ascl1_d10_Dox+_mirVANA_rep1

description: Cy5 is mouse reference


title: Ascl1_d14_Dox-_PSANCAM-_mirVANA_rep1


title: WT H3K36me3 antibody replicate 2

source_name: yeast, wt, H3K36me3 ip, rep2

source_name: yeast, wt, H3K36me3 input, rep2


title: Ascl1_d14_Dox-_PSANCAM-_mirVANA_rep2


title: Smad7_d14_Dox-_PSANCAM+_mirVANA_rep1


title: Ascl1_d7_Dox-_mirVANA_rep1

description: Cy5 is mouse reference


title: Nr2f1_d14_Dox-_PSANCAM+_mirVANA_rep1

description: Cy5 is mouse reference


title: Smad7_d14_Dox-_PSANCAM+_mirVANA_rep2


title: Ascl1_d14_Dox-_PSANCAM+_mirVANA_rep2

description: Biological replicate 2 of 3, Cy3 is mouse RNA and Cy5 is mouse reference


title: Ascl1_d14_Dox-_PSANCAM+_mirVANA_rep3

description: Biological replicate 3 of 3, Cy3 is mouse RNA and Cy5 is mouse reference


title: C106

description:
Gene expression profile of surgically resected material
C106

source_name: patient 106, cancer, LCM

tag: metastasis

tag: tissue


title: Ascl1_d7_Dox+_mirVANA_rep1

description: Cy5 is mouse reference


title: ZR75-1--48hr-Ins--rep2

type: protein

city: Pittsburgh

first: Alison

middle: M

last: Nagle

state: PENNSYLVANIA

address: 204 Craft Ave

department: Pharmacology and Chemical Biology

institute: University of Pittsburgh

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-44

hyb_protocol: The diluted lysates were arrayed on nitrocellulose-coated FAST slides. Each slide was probed with a primary antibody and a biotin-conjugated secondary antibody.

scan_protocol: Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction. Slides were scanned on a flatbed scanner to produce 16-bit tiff image. Spots from tiff images were identified and the density was quantified by MicroVigene.

data_processing:
Relative protein levels for each sample were determined by interpolation of each dilution curves from the standard curve (supercurve) of the slide (antibody).
Supercurve is constructed by a script in R written by Bioinformatics.  These values (given as log2 values) are defined as Supercurve Log 2 value  (format reported in the Metadata). All the data points were normalized for protein loading and transformed to linear value designated as Normalized Linear.

molecule: protein

source_name: breast cancer

growth_protocol: The cell lines were cultured in DMEM, DMEM/F-12, RPMI, L15 or McCoys 5A media supplemented with 10% FBS or horse serum according to the conditions provided by Neve et al. (2006).

extract_protocol: Cell lysates were harvested by washing in ice-cold PBS twice, and lysing immediately in RPPA lysis buffer with protease inhibitor (1% Triton X-100, 50mM Hepes, 150mM NaCl, 1.5mM MgCl2, 1mM EGTA, 100mM NaF, 10mM NaPPi, 10% Glycerol, 1mM Na3VO4, 1mM PMSF, 10g/ml Aprotinin). Lysates were sonicated and centrifuged at 14,000 rpm at 4oC for 10 min. Protein concentrations were determined by BCA. Then, lysates were mixed with 4X SDS/2-ME sample buffer to have final concentration of 1μg/ml. The lysates were boiled for 5 min and diluted two-fold five times.

treatment_protocol: Cells were plated in triplicate 6cm dishes per treatment time point and then switched to serum-free medium (SFM). Cells were serum starved for 24 hours, then treated with 10nM IGF1 or Ins for 5m, 10m, 30m, 6h, 24h, and 48h.

tag: cell line


title: ZR75-1--48hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-45


title: ZR75-1--48hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-43


title: ZR75-1--48hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-42


title: Ascl1_d14_Dox-_PSANCAM+_mirVANA_rep1

description: Biological replicate 1 of 3, Cy3 is mouse RNA and Cy5 is mouse reference


title: ZR75-1--48hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-41


title: Smad7_d14_Dox-_PSANCAM+_Trizol_rep1


title: Nr2f1_d14_Dox-_PSANCAM+_Trizol_rep1

description: Cy5 is mouse reference


title: Ascl1_d14_Dox-_PSANCAM+_Trizol_rep1


title: Smad7_d14_Dox-_PSANCAM+_Trizol_rep2


title: Ascl1_d14_Dox-_PSANCAM+_Trizol_rep2


title: MC1_d3_Dox+_Trizol_rep1

description: Cy5 is mouse reference


title: ZR75-1--48hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-40


title: ZR75-1--48hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-37


title: ZR75-1--48hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-39


title: ZR75-1--48hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-38


title: ZR75-1--24hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-36


title: ZR75-1--24hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-35


title: ZR75-1--24hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-32


title: ZR75-1--24hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-34


title: ZR75-1--24hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-33


title: ZR75-1--24hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-31


title: ZR75-1--24hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-30


title: ZR75-1--6hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-25


title: ZR75-1--24hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-28


title: ZR75-1--24hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-29


title: ZR75-1--6hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-23


title: ZR75-1--6hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-24


title: ZR75-1--30min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-21


title: ZR75-1--6hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-22


title: ZR75-1--30min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-20


title: ZR75-1--30min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-17


title: ZR75-1--10min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-15


title: ZR75-1--10min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-14


title: ZR75-1--10min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-12


title: ZR75-1--10min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-13


title: ZR75-1--5min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-9


title: ZR75-1--6hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-27


title: ZR75-1--10min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-11


title: ZR75-1--5min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-4


title: ZR75-1--5min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-6


title: ZR75-1--5min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-8


title: ZR75-1--5min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-7


title: ZR75-1--30min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-19


title: ZR75-1--6hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-26


title: ZR75-1--5min-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-2


title: ZR75-1--5min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-5


title: ZR75-1--5min-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-3


title: ZR75-1--30min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-18


title: ZR75-1--5min-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-1


title: ZR75-1--30min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-16


title: UACC812--48hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-45


title: UACC812--48hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-41


title: ZR75-1--10min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
ZR75-1-10


title: UACC812--48hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-43


title: UACC812--48hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-44


title: UACC812--48hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-40


title: UACC812--48hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-37


title: UACC812--48hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-42


title: UACC812--48hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-38


title: UACC812--48hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-39


title: UACC812--24hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-35


title: UACC812--24hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-36


title: UACC812--24hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-34


title: UACC812--24hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-30


title: UACC812--24hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-32


title: UACC812--30min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-21


title: UACC812--6hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-22


title: UACC812--24hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-28


title: UACC812--24hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-29


title: UACC812--6hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-25


title: UACC812--24hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-31


title: UACC812--24hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-33


title: UACC812--6hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-27


title: UACC812--6hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-26


title: UACC812--6hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-23


title: UACC812--30min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-20


title: UACC812--30min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-17


title: UACC812--30min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-19


title: UACC812--30min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-18


title: UACC812--30min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-16


title: UACC812--10min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-15


title: UACC812--6hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-24


title: UACC812--10min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-11


title: UACC812--10min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-12


title: UACC812--10min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-14


title: UACC812--5min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-8


title: UACC812--5min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-7


title: UACC812--5min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-9


title: UACC812--5min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-6


title: UACC812--5min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-5


title: UACC812--5min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-4


title: T47D--48hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-45


title: T47D--48hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-44


title: T47D--48hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-42


title: T47D--48hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-43


title: UACC812--5min-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-3


title: UACC812--5min-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-1


title: UACC812--5min-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-2


title: UACC812--10min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-13


title: T47D--48hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-40


title: T47D--48hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-37


title: T47D--48hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-39


title: T47D--48hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-38


title: T47D--24hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-36


title: UACC812--10min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
UACC812-10


title: T47D--24hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-32


title: T47D--48hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-41


title: T47D--24hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-31


title: T47D--24hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-28


title: T47D--6hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-27


title: T47D--24hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-29


title: T47D--24hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-30


title: T47D--24hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-35


title: T47D--24hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-34


title: T47D--6hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-24


title: T47D--6hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-25


title: T47D--6hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-23


title: T47D--6hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-22


title: T47D--30min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-21


title: T47D--24hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-33


title: T47D--30min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-19


title: T47D--30min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-17


title: T47D--10min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-15


title: T47D--30min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-18


title: T47D--30min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-20


title: T47D--10min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-12


title: T47D--10min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-11


title: T47D--6hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-26


title: T47D--5min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-8


title: T47D--30min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-16


title: T47D--5min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-6


title: T47D--5min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-9


title: T47D--10min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-14


title: T47D--5min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-5


title: T47D--10min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-10


title: T47D--5min-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-3


title: T47D--5min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-7


title: T47D--10min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-13


title: SKBr3--48hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-45


title: T47D--5min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-4


title: T47D--5min-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-1


title: SKBr3--48hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-44


title: SKBr3--48hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-43


title: T47D--5min-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
T47D-2


title: SKBr3--48hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-42


title: SKBr3--48hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-38


title: SKBr3--24hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-35


title: SKBr3--48hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-41


title: SKBr3--24hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-36


title: SKBr3--48hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-37


title: SKBr3--24hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-31


title: SKBr3--24hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-28


title: SKBr3--24hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-32


title: SKBr3--24hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-30


title: SKBr3--6hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-23


title: SKBr3--30min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-21


title: SKBr3--30min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-20


title: SKBr3--24hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-29


title: SKBr3--30min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-19


title: SKBr3--24hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-33


title: SKBr3--6hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-24


title: SKBr3--6hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-22


title: SKBr3--48hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-40


title: SKBr3--6hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-26


title: SKBr3--10min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-12


title: SKBr3--10min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-14


title: SKBr3--30min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-16


title: SKBr3--10min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-13


title: SKBr3--48hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-39


title: SKBr3--10min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-15


title: SKBr3--30min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-17


title: SKBr3--24hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-34


title: SKBr3--6hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-27


title: SKBr3--30min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-18


title: SKBr3--6hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-25


title: SKBr3--10min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-10


title: SKBr3--5min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-9


title: SKBr3--5min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-8


title: SKBr3--5min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-7


title: SKBr3--5min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-4


title: SKBr3--5min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-6


title: SKBr3--5min-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-3


title: SKBr3--5min-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-2


title: MDA-MB-468--48hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-44


title: MDA-MB-468--48hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-43


title: MDA-MB-468--48hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-45


title: MDA-MB-468--48hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-39


title: MDA-MB-468--48hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-40


title: MDA-MB-468--48hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-37


title: MDA-MB-468--24hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-36


title: MDA-MB-468--24hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-34


title: MDA-MB-468--24hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-32


title: MDA-MB-468--24hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-33


title: MDA-MB-468--48hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-41


title: MDA-MB-468--24hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-31


title: MDA-MB-468--24hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-35


title: MDA-MB-468--24hr-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-28


title: MDA-MB-468--24hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-29


title: SKBr3--10min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-11


title: MDA-MB-468--6hr-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-27


title: SKBr3--5min-SFM--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-1


title: SKBr3--5min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
SKBr3-5


title: MDA-MB-468--6hr-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-23


title: MDA-MB-468--6hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-24


title: MDA-MB-468--6hr-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-25


title: MDA-MB-468--48hr-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-42


title: MDA-MB-468--6hr-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-26


title: MDA-MB-468--30min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-21


title: MDA-MB-468--48hr-SFM--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-38


title: MDA-MB-468--30min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-20


title: MDA-MB-468--24hr-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-30


title: MDA-MB-468--6hr-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-22


title: MDA-MB-468--30min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-18


title: MDA-MB-468--30min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-17


title: MDA-MB-468--30min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-19


title: MDA-MB-468--30min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-16


title: MDA-MB-468--10min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-10


title: MDA-MB-468--10min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-12


title: MDA-MB-468--10min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-15


title: MDA-MB-468--10min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-11


title: MDA-MB-468--5min-Ins--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-9


title: MDA-MB-468--5min-IGF--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-5


title: MDA-MB-468--10min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-14


title: MDA-MB-468--5min-Ins--rep2

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-8


title: MDA-MB-468--5min-IGF--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-6


title: MDA-MB-468--5min-SFM--rep3

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-3


title: MDA-MB-468--5min-IGF--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-4


title: MDA-MB-468--10min-Ins--rep1

description:
Lysates were two-fold-serial diluted for 5 dilutions (from undiluted to 1:16 dilution) and arrayed on nitrocellulose-coated slide in 11x11 format. Samples were probed with antibodies by CSA amplification approach and visualized by DAB colorimetric reaction.
MDA-MB-468-13