title: EHUH001_ssGP_24h_5_RNA

source_name: Huh cells, ssGP-inoculated, 24h, bioreplicate 5


title: EHUH001_mock_48h_2_RNA

status: Public on May 07 2016

type: RNA

city: Richland

first: Natalie

last: Heller

address: 902 Battelle Blvd.

country: USA

institute: PNNL

hyb_protocol: Hybridization was performed according to Agilent One-Color Microarray-Based Gene Expression Analysis Protocol (Agilent Technology). The labeled cRNAs were purified by RNeasy Mini Kit (Qiagen). The concentration and specific activity of the labeled cRNAs (pmol Cy3/μg cRNA) were measured by NanoDrop ND-1000. 1 μg of each labeled cRNA was fragmented by adding 11 μl 10 × Blocking Agent and 2.2 μl of 25×Fragmentation Buffer, then heated at 60 °C for 30 min, and finally 55 μl 2 × GE Hybridization buffer was added to dilute the labeled cRNA. 100μl of hybridization solution was dispensed into the gasket slide and assembled to the gene expression microarray slide. The slides were incubated for 17 hours at 65°C in an Agilent Hybridization Oven.

scan_protocol: The hybridized arrays were washed, fixed and scanned with using the Agilent DNA Microarray Scanner (part number G2505C).

data_processing: Scanned images were analyzed using Agilent Feature Extraction Software (v11.0.1.1). The limma package for R (available on Bioconductor) was used to perform background correction, quantile normalization (normalizeBetweenArrays), and summarization (avereps) to derive a single normalized intensity value per probe. Outlier samples were detected using PCA and by visual inspection of heatmaps, and all data was re-processed after removing outlier samples. All data processing for each of the biological replicates was performed independently of the other.

molecule: total RNA

organism: Homo sapiens

source_name: Huh cells, mock-inoculated, 48h, bioreplicate 2

label_protocol: Total RNA was labeled according to Agilent’s Quick Amp Labeling protocol as part of the Agilent One-Color Microarray-Based Gene Expression Analysis Protocol (Agilent Technology). Total RNA from each sample was linearly amplified and labeled with Cy3-UTP.

growth_protocol: Cells and virus were grown on 10% FBS DMEM supplemented with L-glut, Pen/Strep, nonessential amino acids and sodium pyruvate.

extract_protocol: RNA was extracted with 1mL of Trizol.

treatment_protocol: Cells were infected with a multiplicity of infection of 3.

tag: cell line

value: Huh

tag: time

tag: virus

value: mock


title: EHUH001_ssGP_48h_4_RNA

source_name: Huh cells, ssGP-inoculated, 48h, bioreplicate 4

value: ssGP


title: EHUH001_mock_48h_1_RNA

source_name: Huh cells, mock-inoculated, 48h, bioreplicate 1


title: EHUH001_mock_48h_5_RNA

source_name: Huh cells, mock-inoculated, 48h, bioreplicate 5


title: EHUH001_ssGP_24h_3_RNA

source_name: Huh cells, ssGP-inoculated, 24h, bioreplicate 3


title: EHUH001_ssGP_48h_5_RNA

source_name: Huh cells, ssGP-inoculated, 48h, bioreplicate 5


title: EHUH001_ssGP_24h_2_RNA

source_name: Huh cells, ssGP-inoculated, 24h, bioreplicate 2


title: EHUH001_ssGP_24h_4_RNA

source_name: Huh cells, ssGP-inoculated, 24h, bioreplicate 4


title: ICL104_Cal04_0hr_RNA_1

status: Public on May 26 2016

growth_protocol: SCL002.0P: Maintenance, Plating, and Virus Infection of Calu-3 cells

extract_protocol: TCL001.0P: Preparation of Samples from Calu-3 cells for Isolation of RNA

treatment_protocol: Cells were infected with an MOI of 3. After infection, wash and replace with 2 ml of DMEM-F12 + .3% BSA and 1 ug/ml TPCK-trypsin.

value: wild-type pandemic H1N1 (A/California/04/2009); Cal04


title: EHUH001_mucin_24h_4_RNA

source_name: Huh cells, mucin-inoculated, 24h, bioreplicate 4

value: mucin


title: EHUH001_ssGP_24h_1_RNA

source_name: Huh cells, ssGP-inoculated, 24h, bioreplicate 1


title: EHUH001_mucin_24h_3_RNA

source_name: Huh cells, mucin-inoculated, 24h, bioreplicate 3


title: EHUH001_wild_24h_5_RNA

source_name: Huh cells, wild-inoculated, 24h, bioreplicate 5

value: wild


title: EHUH001_mucin_24h_2_RNA

source_name: Huh cells, mucin-inoculated, 24h, bioreplicate 2


title: EHUH001_mucin_24h_1_RNA

source_name: Huh cells, mucin-inoculated, 24h, bioreplicate 1


title: EHUH001_wild_24h_1_RNA

source_name: Huh cells, wild-inoculated, 24h, bioreplicate 1


title: EHUH001_wild_24h_4_RNA

source_name: Huh cells, wild-inoculated, 24h, bioreplicate 4


title: EHUH001_ssGP_18h_4_RNA

source_name: Huh cells, ssGP-inoculated, 18h, bioreplicate 4


title: EHUH001_mock_24h_5_RNA

source_name: Huh cells, mock-inoculated, 24h, bioreplicate 5


title: EHUH001_mock_24h_4_RNA

source_name: Huh cells, mock-inoculated, 24h, bioreplicate 4


title: EHUH001_wild_24h_2_RNA

source_name: Huh cells, wild-inoculated, 24h, bioreplicate 2


title: EHUH001_mucin_24h_5_RNA

source_name: Huh cells, mucin-inoculated, 24h, bioreplicate 5


title: EHUH001_mock_24h_3_RNA

source_name: Huh cells, mock-inoculated, 24h, bioreplicate 3


title: EHUH001_ssGP_18h_5_RNA

source_name: Huh cells, ssGP-inoculated, 18h, bioreplicate 5


title: EHUH001_ssGP_18h_1_RNA

source_name: Huh cells, ssGP-inoculated, 18h, bioreplicate 1


title: EHUH001_wild_24h_3_RNA

source_name: Huh cells, wild-inoculated, 24h, bioreplicate 3


title: EHUH001_ssGP_18h_2_RNA

source_name: Huh cells, ssGP-inoculated, 18h, bioreplicate 2


title: EHUH001_mucin_18h_3_RNA

source_name: Huh cells, mucin-inoculated, 18h, bioreplicate 3


title: EHUH001_mucin_18h_4_RNA

source_name: Huh cells, mucin-inoculated, 18h, bioreplicate 4


title: EHUH001_mock_24h_2_RNA

source_name: Huh cells, mock-inoculated, 24h, bioreplicate 2


title: EHUH001_mucin_18h_1_RNA

source_name: Huh cells, mucin-inoculated, 18h, bioreplicate 1


title: EHUH001_mucin_18h_2_RNA

source_name: Huh cells, mucin-inoculated, 18h, bioreplicate 2


title: EHUH001_ssGP_18h_3_RNA

source_name: Huh cells, ssGP-inoculated, 18h, bioreplicate 3


title: EHUH001_mucin_18h_5_RNA

source_name: Huh cells, mucin-inoculated, 18h, bioreplicate 5


title: EHUH001_wild_18h_4_RNA

source_name: Huh cells, wild-inoculated, 18h, bioreplicate 4


title: EHUH001_mock_24h_1_RNA

source_name: Huh cells, mock-inoculated, 24h, bioreplicate 1


title: EHUH001_wild_18h_3_RNA

source_name: Huh cells, wild-inoculated, 18h, bioreplicate 3


title: EHUH001_wild_18h_1_RNA

source_name: Huh cells, wild-inoculated, 18h, bioreplicate 1


title: EHUH001_mock_18h_5_RNA

source_name: Huh cells, mock-inoculated, 18h, bioreplicate 5


title: EHUH001_ssGP_8h_3_RNA

source_name: Huh cells, ssGP-inoculated, 8h, bioreplicate 3


title: EHUH001_mock_18h_1_RNA

source_name: Huh cells, mock-inoculated, 18h, bioreplicate 1


title: EHUH001_mock_18h_4_RNA

source_name: Huh cells, mock-inoculated, 18h, bioreplicate 4


title: EHUH001_wild_18h_5_RNA

source_name: Huh cells, wild-inoculated, 18h, bioreplicate 5


title: EHUH001_ssGP_8h_4_RNA

source_name: Huh cells, ssGP-inoculated, 8h, bioreplicate 4


title: EHUH001_ssGP_8h_2_RNA

source_name: Huh cells, ssGP-inoculated, 8h, bioreplicate 2


title: EHUH001_mock_18h_3_RNA

source_name: Huh cells, mock-inoculated, 18h, bioreplicate 3


title: EHUH001_ssGP_8h_1_RNA

source_name: Huh cells, ssGP-inoculated, 8h, bioreplicate 1


title: EHUH001_wild_18h_2_RNA

source_name: Huh cells, wild-inoculated, 18h, bioreplicate 2


title: EHUH001_mock_18h_2_RNA

source_name: Huh cells, mock-inoculated, 18h, bioreplicate 2


title: EHUH001_mucin_8h_5_RNA

source_name: Huh cells, mucin-inoculated, 8h, bioreplicate 5


title: EHUH001_mucin_8h_2_RNA

source_name: Huh cells, mucin-inoculated, 8h, bioreplicate 2


title: EHUH001_mucin_8h_1_RNA

source_name: Huh cells, mucin-inoculated, 8h, bioreplicate 1


title: EHUH001_mucin_8h_4_RNA

source_name: Huh cells, mucin-inoculated, 8h, bioreplicate 4


title: EHUH001_wild_8h_2_RNA

source_name: Huh cells, wild-inoculated, 8h, bioreplicate 2


title: EHUH001_wild_8h_4_RNA

source_name: Huh cells, wild-inoculated, 8h, bioreplicate 4


title: EHUH001_ssGP_8h_5_RNA

source_name: Huh cells, ssGP-inoculated, 8h, bioreplicate 5


title: EHUH001_wild_8h_5_RNA

source_name: Huh cells, wild-inoculated, 8h, bioreplicate 5


title: EHUH001_mock_8h_3_RNA

source_name: Huh cells, mock-inoculated, 8h, bioreplicate 3


title: EHUH001_ssGP_00h_4_RNA

source_name: Huh cells, ssGP-inoculated, 00h, bioreplicate 4


title: EHUH001_mucin_8h_3_RNA

source_name: Huh cells, mucin-inoculated, 8h, bioreplicate 3


title: EHUH001_ssGP_00h_5_RNA

source_name: Huh cells, ssGP-inoculated, 00h, bioreplicate 5


title: EHUH001_ssGP_00h_1_RNA

source_name: Huh cells, ssGP-inoculated, 00h, bioreplicate 1


title: EHUH001_wild_8h_3_RNA

source_name: Huh cells, wild-inoculated, 8h, bioreplicate 3


title: EHUH001_mock_8h_5_RNA

source_name: Huh cells, mock-inoculated, 8h, bioreplicate 5


title: EHUH001_ssGP_00h_3_RNA

source_name: Huh cells, ssGP-inoculated, 00h, bioreplicate 3


title: EHUH001_mock_8h_1_RNA

source_name: Huh cells, mock-inoculated, 8h, bioreplicate 1


title: EHUH001_mock_8h_4_RNA

source_name: Huh cells, mock-inoculated, 8h, bioreplicate 4


title: EHUH001_mucin_00h_4_RNA

source_name: Huh cells, mucin-inoculated, 00h, bioreplicate 4


title: EHUH001_mucin_48h_3_RNA

source_name: Huh cells, mucin-inoculated, 48h, bioreplicate 3


title: EHUH001_mock_8h_2_RNA

source_name: Huh cells, mock-inoculated, 8h, bioreplicate 2


title: EHUH001_mucin_00h_5_RNA

source_name: Huh cells, mucin-inoculated, 00h, bioreplicate 5


title: EHUH001_mucin_00h_1_RNA

source_name: Huh cells, mucin-inoculated, 00h, bioreplicate 1


title: EHUH001_mucin_00h_2_RNA

source_name: Huh cells, mucin-inoculated, 00h, bioreplicate 2


title: EHUH001_ssGP_00h_2_RNA

source_name: Huh cells, ssGP-inoculated, 00h, bioreplicate 2


title: ICL104_Cal04_36hr_microRNA_4

tag: molecule subtype

value: microRNA


title: EHUH001_mucin_00h_3_RNA

source_name: Huh cells, mucin-inoculated, 00h, bioreplicate 3


title: EHUH001_wild_8h_1_RNA

source_name: Huh cells, wild-inoculated, 8h, bioreplicate 1


title: ICL104_Cal04_36hr_microRNA_3


title: EHUH001_wild_00h_3_RNA

source_name: Huh cells, wild-inoculated, 00h, bioreplicate 3


title: EHUH001_wild_00h_5_RNA

source_name: Huh cells, wild-inoculated, 00h, bioreplicate 5


title: EHUH001_wild_00h_2_RNA

source_name: Huh cells, wild-inoculated, 00h, bioreplicate 2


title: EHUH001_mock_00h_5_RNA

source_name: Huh cells, mock-inoculated, 00h, bioreplicate 5


title: EHUH001_wild_00h_1_RNA

source_name: Huh cells, wild-inoculated, 00h, bioreplicate 1


title: ICL104_Mock_36hr_microRNA_5

value: Mock


title: EHUH001_mock_00h_1_RNA

source_name: Huh cells, mock-inoculated, 00h, bioreplicate 1


title: ICL104_Mock_36hr_microRNA_2


title: ICL104_Cal04_36hr_microRNA_5


title: EHUH001_mock_00h_3_RNA

source_name: Huh cells, mock-inoculated, 00h, bioreplicate 3


title: EHUH001_wild_00h_4_RNA

source_name: Huh cells, wild-inoculated, 00h, bioreplicate 4


title: EHUH001_mock_00h_2_RNA

source_name: Huh cells, mock-inoculated, 00h, bioreplicate 2


title: ICL104_Cal04_36hr_microRNA_2


title: ICL104_Mock_36hr_microRNA_1


title: ICL104_Cal04_24hr_microRNA_5


title: ICL104_Mock_36hr_microRNA_3


title: ICL104_Cal04_24hr_microRNA_3


title: EHUH001_ssGP_0h_2_RNA

source_name: Huh cells, ssGP-inoculated, 0h, bioreplicate 2


title: EHUH001_ssGP_0h_4_RNA

source_name: Huh cells, ssGP-inoculated, 0h, bioreplicate 4


title: EHUH001_ssGP_0h_3_RNA

source_name: Huh cells, ssGP-inoculated, 0h, bioreplicate 3


title: EHUH001_mock_00h_4_RNA

source_name: Huh cells, mock-inoculated, 00h, bioreplicate 4


title: ICL104_Cal04_24hr_microRNA_1


title: ICL104_Mock_24hr_microRNA_5


title: ICL104_Cal04_24hr_microRNA_2


title: ICL104_Mock_36hr_microRNA_4


title: ICL104_Mock_24hr_microRNA_4


title: ICL104_Mock_24hr_microRNA_2


title: ICL104_Cal04_36hr_microRNA_1


title: ICL104_Mock_24hr_microRNA_1


title: ICL104_Cal04_24hr_microRNA_4


title: ICL104_Cal04_12hr_microRNA_5


title: ICL104_Cal04_12hr_microRNA_4


title: ICL104_Cal04_12hr_microRNA_2


title: EHUH001_ssGP_0h_1_RNA

source_name: Huh cells, ssGP-inoculated, 0h, bioreplicate 1


title: EHUH001_mucin_0h_5_RNA

source_name: Huh cells, mucin-inoculated, 0h, bioreplicate 5


title: EHUH001_mucin_0h_2_RNA

source_name: Huh cells, mucin-inoculated, 0h, bioreplicate 2


title: ICL104_Cal04_12hr_microRNA_1


title: EHUH001_mucin_0h_4_RNA

source_name: Huh cells, mucin-inoculated, 0h, bioreplicate 4


title: ICL104_Cal04_12hr_microRNA_3


title: EHUH001_mucin_0h_1_RNA

source_name: Huh cells, mucin-inoculated, 0h, bioreplicate 1


title: EHUH001_ssGP_48h_3_microRNA

description: microRNA

source_name: Huh cells, ssGP-inoculated, 48h, bioreplicate 3


title: ICL104_Mock_12hr_microRNA_1


title: ICL104_Mock_12hr_microRNA_5


title: EHUH001_wild_0h_5_RNA

source_name: Huh cells, wild-inoculated, 0h, bioreplicate 5


title: ICL104_Mock_12hr_microRNA_3


title: ICL104_Mock_12hr_microRNA_4


title: ICL104_Mock_12hr_microRNA_2


title: EHUH001_mucin_48h_5_microRNA

description: microRNA

source_name: Huh cells, mucin-inoculated, 48h, bioreplicate 5


title: EHUH001_mucin_48h_3_microRNA

description: microRNA

source_name: Huh cells, mucin-inoculated, 48h, bioreplicate 3


title: EHUH001_ssGP_48h_5_microRNA

description: microRNA

source_name: Huh cells, ssGP-inoculated, 48h, bioreplicate 5


title: EHUH001_wild_0h_3_RNA

source_name: Huh cells, wild-inoculated, 0h, bioreplicate 3


title: EHUH001_mucin_48h_2_microRNA

description: microRNA

source_name: Huh cells, mucin-inoculated, 48h, bioreplicate 2


title: EHUH001_mucin_0h_3_RNA

source_name: Huh cells, mucin-inoculated, 0h, bioreplicate 3


title: EHUH001_wild_0h_4_RNA

source_name: Huh cells, wild-inoculated, 0h, bioreplicate 4


title: EHUH001_wild_48h_5_microRNA

description: microRNA

source_name: Huh cells, wild-inoculated, 48h, bioreplicate 5


title: EHUH001_mock_0h_4_RNA

source_name: Huh cells, mock-inoculated, 0h, bioreplicate 4


title: EHUH001_wild_0h_2_RNA

source_name: Huh cells, wild-inoculated, 0h, bioreplicate 2


title: EHUH001_ssGP_48h_4_microRNA

description: microRNA

source_name: Huh cells, ssGP-inoculated, 48h, bioreplicate 4


title: EHUH001_mock_0h_3_RNA

source_name: Huh cells, mock-inoculated, 0h, bioreplicate 3


title: EHUH001_mock_0h_2_RNA

source_name: Huh cells, mock-inoculated, 0h, bioreplicate 2


title: EHUH001_ssGP_48h_1_microRNA

description: microRNA

source_name: Huh cells, ssGP-inoculated, 48h, bioreplicate 1


title: EHUH001_ssGP_48h_2_microRNA

description: microRNA

source_name: Huh cells, ssGP-inoculated, 48h, bioreplicate 2


title: EHUH001_mucin_48h_4_microRNA

description: microRNA

source_name: Huh cells, mucin-inoculated, 48h, bioreplicate 4


title: EHUH001_mucin_48h_1_microRNA

description: microRNA

source_name: Huh cells, mucin-inoculated, 48h, bioreplicate 1


title: EHUH001_wild_0h_1_RNA

source_name: Huh cells, wild-inoculated, 0h, bioreplicate 1


title: EHUH001_wild_48h_2_microRNA

description: microRNA

source_name: Huh cells, wild-inoculated, 48h, bioreplicate 2


title: EHUH001_wild_48h_4_microRNA

description: microRNA

source_name: Huh cells, wild-inoculated, 48h, bioreplicate 4


title: EHUH001_mock_0h_5_RNA

source_name: Huh cells, mock-inoculated, 0h, bioreplicate 5


title: EHUH001_mock_48h_4_microRNA

description: microRNA

source_name: Huh cells, mock-inoculated, 48h, bioreplicate 4


title: EHUH001_mock_48h_3_microRNA

description: microRNA

source_name: Huh cells, mock-inoculated, 48h, bioreplicate 3


title: EHUH001_wild_48h_3_microRNA

description: microRNA

source_name: Huh cells, wild-inoculated, 48h, bioreplicate 3


title: EHUH001_ssGP_24h_4_microRNA

description: microRNA

source_name: Huh cells, ssGP-inoculated, 24h, bioreplicate 4


title: EHUH001_mock_48h_2_microRNA

description: microRNA

source_name: Huh cells, mock-inoculated, 48h, bioreplicate 2


title: EHUH001_wild_48h_1_microRNA

description: microRNA

source_name: Huh cells, wild-inoculated, 48h, bioreplicate 1


title: EHUH001_ssGP_24h_3_microRNA

description: microRNA

source_name: Huh cells, ssGP-inoculated, 24h, bioreplicate 3


title: EHUH001_mucin_24h_3_microRNA

description: microRNA

source_name: Huh cells, mucin-inoculated, 24h, bioreplicate 3


title: EHUH001_mock_48h_1_microRNA

description: microRNA

source_name: Huh cells, mock-inoculated, 48h, bioreplicate 1


title: EHUH001_ssGP_24h_1_microRNA

description: microRNA

source_name: Huh cells, ssGP-inoculated, 24h, bioreplicate 1


title: EHUH001_mucin_24h_4_microRNA

description: microRNA

source_name: Huh cells, mucin-inoculated, 24h, bioreplicate 4


title: EHUH001_ssGP_24h_2_microRNA

description: microRNA

source_name: Huh cells, ssGP-inoculated, 24h, bioreplicate 2


title: EHUH001_mock_48h_5_microRNA

description: microRNA

source_name: Huh cells, mock-inoculated, 48h, bioreplicate 5


title: EHUH001_ssGP_24h_5_microRNA

description: microRNA

source_name: Huh cells, ssGP-inoculated, 24h, bioreplicate 5


title: EHUH001_mucin_24h_2_microRNA

description: microRNA

source_name: Huh cells, mucin-inoculated, 24h, bioreplicate 2


title: EHUH001_mucin_24h_5_microRNA

description: microRNA

source_name: Huh cells, mucin-inoculated, 24h, bioreplicate 5


title: EHUH001_wild_24h_1_microRNA

description: microRNA

source_name: Huh cells, wild-inoculated, 24h, bioreplicate 1


title: EHUH001_mock_24h_4_microRNA

description: microRNA

source_name: Huh cells, mock-inoculated, 24h, bioreplicate 4


title: EHUH001_mock_0h_1_RNA

source_name: Huh cells, mock-inoculated, 0h, bioreplicate 1


title: EHUH001_mock_24h_5_microRNA

description: microRNA

source_name: Huh cells, mock-inoculated, 24h, bioreplicate 5


title: EHUH001_wild_24h_5_microRNA

description: microRNA

source_name: Huh cells, wild-inoculated, 24h, bioreplicate 5


title: EHUH001_mucin_24h_1_microRNA

description: microRNA

source_name: Huh cells, mucin-inoculated, 24h, bioreplicate 1


title: EHUH001_wild_24h_4_microRNA

description: microRNA

source_name: Huh cells, wild-inoculated, 24h, bioreplicate 4


title: EHUH001_mock_24h_1_microRNA

description: microRNA

source_name: Huh cells, mock-inoculated, 24h, bioreplicate 1


title: EHUH001_ssGP_18h_4_microRNA

description: microRNA

source_name: Huh cells, ssGP-inoculated, 18h, bioreplicate 4


title: EHUH001_wild_24h_3_microRNA

description: microRNA

source_name: Huh cells, wild-inoculated, 24h, bioreplicate 3


title: EHUH001_wild_24h_2_microRNA

description: microRNA

source_name: Huh cells, wild-inoculated, 24h, bioreplicate 2


title: EHUH001_mucin_18h_5_microRNA

description: microRNA

source_name: Huh cells, mucin-inoculated, 18h, bioreplicate 5


title: EHUH001_ssGP_18h_1_microRNA

description: microRNA

source_name: Huh cells, ssGP-inoculated, 18h, bioreplicate 1


title: EHUH001_ssGP_18h_3_microRNA

description: microRNA

source_name: Huh cells, ssGP-inoculated, 18h, bioreplicate 3


title: EHUH001_mucin_18h_1_microRNA

description: microRNA

source_name: Huh cells, mucin-inoculated, 18h, bioreplicate 1


title: EHUH001_mucin_18h_4_microRNA

description: microRNA

source_name: Huh cells, mucin-inoculated, 18h, bioreplicate 4


title: EHUH001_mucin_18h_3_microRNA

description: microRNA

source_name: Huh cells, mucin-inoculated, 18h, bioreplicate 3


title: EHUH001_mucin_18h_2_microRNA

description: microRNA

source_name: Huh cells, mucin-inoculated, 18h, bioreplicate 2


title: EHUH001_mock_24h_3_microRNA

description: microRNA

source_name: Huh cells, mock-inoculated, 24h, bioreplicate 3


title: EHUH001_mock_24h_2_microRNA

description: microRNA

source_name: Huh cells, mock-inoculated, 24h, bioreplicate 2


title: EHUH001_wild_18h_2_microRNA

description: microRNA

source_name: Huh cells, wild-inoculated, 18h, bioreplicate 2


title: EHUH001_mock_18h_5_microRNA

description: microRNA

source_name: Huh cells, mock-inoculated, 18h, bioreplicate 5


title: EHUH001_wild_18h_4_microRNA

description: microRNA

source_name: Huh cells, wild-inoculated, 18h, bioreplicate 4


title: EHUH001_wild_18h_3_microRNA

description: microRNA

source_name: Huh cells, wild-inoculated, 18h, bioreplicate 3


title: EHUH001_mock_18h_2_microRNA

description: microRNA

source_name: Huh cells, mock-inoculated, 18h, bioreplicate 2


title: EHUH001_wild_18h_5_microRNA

description: microRNA

source_name: Huh cells, wild-inoculated, 18h, bioreplicate 5


title: EHUH001_mock_18h_4_microRNA

description: microRNA

source_name: Huh cells, mock-inoculated, 18h, bioreplicate 4


title: EHUH001_mock_18h_1_microRNA

description: microRNA

source_name: Huh cells, mock-inoculated, 18h, bioreplicate 1


title: EHUH001_ssGP_18h_2_microRNA

description: microRNA

source_name: Huh cells, ssGP-inoculated, 18h, bioreplicate 2


title: EHUH001_wild_18h_1_microRNA

description: microRNA

source_name: Huh cells, wild-inoculated, 18h, bioreplicate 1


title: EHUH001_ssGP_8h_3_microRNA

description: microRNA

source_name: Huh cells, ssGP-inoculated, 8h, bioreplicate 3


title: EHUH001_ssGP_8h_5_microRNA

description: microRNA

source_name: Huh cells, ssGP-inoculated, 8h, bioreplicate 5


title: EHUH001_ssGP_8h_4_microRNA

description: microRNA

source_name: Huh cells, ssGP-inoculated, 8h, bioreplicate 4


title: EHUH001_mucin_8h_3_microRNA

description: microRNA

source_name: Huh cells, mucin-inoculated, 8h, bioreplicate 3


title: EHUH001_mucin_8h_4_microRNA

description: microRNA

source_name: Huh cells, mucin-inoculated, 8h, bioreplicate 4


title: EHUH001_mucin_8h_5_microRNA

description: microRNA

source_name: Huh cells, mucin-inoculated, 8h, bioreplicate 5


title: EHUH001_ssGP_8h_2_microRNA

description: microRNA

source_name: Huh cells, ssGP-inoculated, 8h, bioreplicate 2


title: EHUH001_ssGP_8h_1_microRNA

description: microRNA

source_name: Huh cells, ssGP-inoculated, 8h, bioreplicate 1


title: EHUH001_wild_8h_4_microRNA

description: microRNA

source_name: Huh cells, wild-inoculated, 8h, bioreplicate 4


title: EHUH001_mock_18h_3_microRNA

description: microRNA

source_name: Huh cells, mock-inoculated, 18h, bioreplicate 3


title: EHUH001_mucin_8h_1_microRNA

description: microRNA

source_name: Huh cells, mucin-inoculated, 8h, bioreplicate 1


title: EHUH001_wild_8h_5_microRNA

description: microRNA

source_name: Huh cells, wild-inoculated, 8h, bioreplicate 5


title: EHUH001_mock_8h_5_microRNA

description: microRNA

source_name: Huh cells, mock-inoculated, 8h, bioreplicate 5


title: EHUH001_mock_8h_4_microRNA

description: microRNA

source_name: Huh cells, mock-inoculated, 8h, bioreplicate 4


title: EHUH001_mucin_8h_2_microRNA

description: microRNA

source_name: Huh cells, mucin-inoculated, 8h, bioreplicate 2


title: EHUH001_wild_8h_3_microRNA

description: microRNA

source_name: Huh cells, wild-inoculated, 8h, bioreplicate 3


title: EHUH001_mock_8h_3_microRNA

description: microRNA

source_name: Huh cells, mock-inoculated, 8h, bioreplicate 3


title: EHUH001_wild_8h_1_microRNA

description: microRNA

source_name: Huh cells, wild-inoculated, 8h, bioreplicate 1


title: MFB001_Mock_36h_microRNA_E

status: Public on Apr 28 2016

source_name: Primary human fibroblasts, mock-inoculated, 36h, bioreplicate 5

treatment_protocol: MERSCOV003.0P: Cells were infected with a multiplicity of infection of 5 PFU.

tag: cell type

value: primary fibroblast


title: MFB001_Mock_36h_microRNA_B

source_name: Primary human fibroblasts, mock-inoculated, 36h, bioreplicate 2


title: MFB001_Mock_36h_microRNA_C

source_name: Primary human fibroblasts, mock-inoculated, 36h, bioreplicate 3


title: MFB001_Mock_36h_microRNA_D

source_name: Primary human fibroblasts, mock-inoculated, 36h, bioreplicate 4


title: EHUH001_wild_8h_2_microRNA

description: microRNA

source_name: Huh cells, wild-inoculated, 8h, bioreplicate 2


title: MFB001_icMERS_36h_microRNA_A

source_name: Primary human fibroblasts, icMERS-inoculated, 36h, bioreplicate 1

value: icMERS


title: MFB001_icMERS_36h_microRNA_B

source_name: Primary human fibroblasts, icMERS-inoculated, 36h, bioreplicate 2


title: MFB001_Mock_24h_microRNA_D

source_name: Primary human fibroblasts, mock-inoculated, 24h, bioreplicate 4


title: MFB001_icMERS_36h_microRNA_C

source_name: Primary human fibroblasts, icMERS-inoculated, 36h, bioreplicate 3


title: MFB001_icMERS_36h_microRNA_D

source_name: Primary human fibroblasts, icMERS-inoculated, 36h, bioreplicate 4


title: MFB001_Mock_24h_microRNA_E

source_name: Primary human fibroblasts, mock-inoculated, 24h, bioreplicate 5


title: MFB001_Mock_24h_microRNA_B

source_name: Primary human fibroblasts, mock-inoculated, 24h, bioreplicate 2


title: MFB001_Mock_24h_microRNA_A

source_name: Primary human fibroblasts, mock-inoculated, 24h, bioreplicate 1


title: MFB001_icMERS_24h_microRNA_E

source_name: Primary human fibroblasts, icMERS-inoculated, 24h, bioreplicate 5


title: MFB001_icMERS_36h_microRNA_E

source_name: Primary human fibroblasts, icMERS-inoculated, 36h, bioreplicate 5


title: MFB001_Mock_24h_microRNA_C

source_name: Primary human fibroblasts, mock-inoculated, 24h, bioreplicate 3


title: MFB001_icMERS_24h_microRNA_D

source_name: Primary human fibroblasts, icMERS-inoculated, 24h, bioreplicate 4


title: MFB001_icMERS_24h_microRNA_B

source_name: Primary human fibroblasts, icMERS-inoculated, 24h, bioreplicate 2


title: MFB001_icMERS_12h_microRNA_C

source_name: Primary human fibroblasts, icMERS-inoculated, 12h, bioreplicate 3


title: MFB001_icMERS_12h_microRNA_D

source_name: Primary human fibroblasts, icMERS-inoculated, 12h, bioreplicate 4


title: MFB001_Mock_12h_microRNA_C

source_name: Primary human fibroblasts, mock-inoculated, 12h, bioreplicate 3


title: MFB001_Mock_12h_microRNA_D

source_name: Primary human fibroblasts, mock-inoculated, 12h, bioreplicate 4


title: MFB001_Mock_12h_microRNA_A

source_name: Primary human fibroblasts, mock-inoculated, 12h, bioreplicate 1


title: MFB001_Mock_12h_microRNA_B

source_name: Primary human fibroblasts, mock-inoculated, 12h, bioreplicate 2


title: MFB001_Mock_12h_microRNA_E

source_name: Primary human fibroblasts, mock-inoculated, 12h, bioreplicate 5


title: MFB001_icMERS_24h_microRNA_A

source_name: Primary human fibroblasts, icMERS-inoculated, 24h, bioreplicate 1


title: Gemcitabine Resistant Panc1 (Panc1-GR4)

status: Public on Apr 26 2016

city: Kamakura

first: Satoshi

last: Kondo

state: Kanagawa

address: Tebiro 6-10-1

department: New Projects Development Division

country: Japan

institute: Toray Industries,Inc.

description: MicroRNA data expression from gemcitabine-resistant Panc1 cell clones

hyb_protocol: Hybridized for 16 h at 37 C with rotary shake (250 rpm). Hybridization buffer and washing protocol was followed by the protocol supplied by TORAY Industries, Inc..

data_processing: The raw data of each spot was normalized by substitution with a mean intensity of the background signal determined by all blank spots’ signal intensities of 95% confidence intervals. Measurements of both duplicate spots with the signal intensities greater than 2 standard deviations (SD) of the background signal intensity were considered to be valid. A relative expression level of a given miRNA was calculated by comparing the signal intensities of the averaged valid spots with their mean value throughout the microarray experiments. 3D-Gene Scanner ((Toray Industries Inc., Tokyo, Japan) was used for scanning. Images were quantified using Extraction(Toray Industries Inc., Tokyo, Japan).

source_name: Panc1 human pancreatic cancer cell line, gemcitabine resistant

label_protocol: Extracted total RNA was labeled with Hy5 using the miRCURY LNA Array miR labeling kit (Exiqon, Vedbaek, Denmark)

growth_protocol: Cells were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum (FBS) with antibiotics and incubated at 37 C in a humidified incubator with 5% CO2.

extract_protocol: Total RNA was isolated from parental Panc1 and gemcitabine resistance Panc1 cell lines using Trizol reagent (Invitrogen) according to the manufacturer’s instructions.

treatment_protocol: GEM-resistant cells were generated by exposure to gradually increasing concentrations of the reagent for 2 months. Parental Panc1cells (Panc1-P) were exposed to GEM at an initial concentration of 2 ng/ml. When the cells adapted, the GEM concentration was increased. The final GEM concentration was 30 ng/ml. Using this process, we successfully generated GEM-resistant cells. Limiting the dilution of the established cells allowed the cloning of the GEM-resistant Panc1cells, and the three independent clones (Panc1-GRs: Panc1-GR1, -GR2, and –GR3) were used in the present experiments.

tag: treatment

value: Gemcitabine resistant Panc1 derivative generated from the parental Panc1 line by consecutive treatments with 30ng/ml gemcitabine over a period of 2 months


title: Gemcitabine Resistant Panc1 (Panc1-GR3)

scan_protocol: 3D-Gene Scanner ((Toray Industries Inc., Tokyo, Japan) was used for scanning. Images were quantified using Extraction(Toray Industries Inc., Tokyo, Japan).


title: MFB001_icMERS_12h_microRNA_B

source_name: Primary human fibroblasts, icMERS-inoculated, 12h, bioreplicate 2


title: Gemcitabine Resistant Panc1 (Panc1-GR3)

description: mRNA data expression from gemcitabine-resistant Panc1 cell clones

data_processing: The raw data of each spot was normalized by substitution with a mean intensity of the background signal determined by all blank spots’ signal intensities of 95% confidence intervals.

label_protocol: cDNA and Amino Allyl aRNA was synthesis by Amino Allyl MessageAmp II aRNA Amplification Kit (Ambion#1753). CyeDye Coupling and fragmentation were performed as the protocol supplied by TORAY Industries, Inc..

growth_protocol: Cells were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum (FBS) with antibiotics and incubated at 37°C in a humidified incubator with 5% CO2.

treatment_protocol: GEM-resistant cells were generated by exposure to gradually increasing concentrations of the reagent for 2 months.  Parental Panc1 cells (Panc1-P) were exposed to GEM at an initial concentration of 2 ng/ml.  When the cells adapted, the GEM concentration was increased.  The final GEM concentration was 30 ng/ml.  Using this process, we successfully generated GEM-resistant cells.  Limiting the dilution of the established cells allowed the cloning of the GEM-resistant Panc1 cells, and the three independent clones (Panc1-GRs: Panc1-GR1, -GR2, and –GR3) were used in the present experiments.


title: MFB001_icMERS_36h_RNA_D

status: Public on Apr 17 2016

source_name: Primary human fibroblasts, icMERS-inoculated, 36h, bioreplicate 4


title: MFB001_icMERS_36h_RNA_A

source_name: Primary human fibroblasts, icMERS-inoculated, 36h, bioreplicate 1


title: MFB001_Mock_48h_RNA_C

source_name: Primary human fibroblasts, mock-inoculated, 48h, bioreplicate 3


title: Gemcitabine Resistant Panc1 (Panc1-GR4)


title: MFB001_Mock_36h_RNA_B

source_name: Primary human fibroblasts, mock-inoculated, 36h, bioreplicate 2


title: MFB001_icMERS_48h_RNA_D

source_name: Primary human fibroblasts, icMERS-inoculated, 48h, bioreplicate 4


title: MFB001_Mock_36h_RNA_C

source_name: Primary human fibroblasts, mock-inoculated, 36h, bioreplicate 3


title: MFB001_icMERS_36h_RNA_C

source_name: Primary human fibroblasts, icMERS-inoculated, 36h, bioreplicate 3


title: MFB001_Mock_24h_RNA_D

source_name: Primary human fibroblasts, mock-inoculated, 24h, bioreplicate 4


title: MFB001_Mock_48h_RNA_E

source_name: Primary human fibroblasts, mock-inoculated, 48h, bioreplicate 5


title: MFB001_Mock_36h_RNA_D

source_name: Primary human fibroblasts, mock-inoculated, 36h, bioreplicate 4


title: MFB001_icMERS_48h_RNA_E

source_name: Primary human fibroblasts, icMERS-inoculated, 48h, bioreplicate 5


title: MFB001_Mock_24h_RNA_C

source_name: Primary human fibroblasts, mock-inoculated, 24h, bioreplicate 3


title: MFB001_Mock_48h_RNA_A

source_name: Primary human fibroblasts, mock-inoculated, 48h, bioreplicate 1


title: MFB001_Mock_48h_RNA_B

source_name: Primary human fibroblasts, mock-inoculated, 48h, bioreplicate 2


title: MFB001_Mock_48h_RNA_D

source_name: Primary human fibroblasts, mock-inoculated, 48h, bioreplicate 4


title: MFB001_icMERS_36h_RNA_B

source_name: Primary human fibroblasts, icMERS-inoculated, 36h, bioreplicate 2


title: MFB001_Mock_36h_RNA_A

source_name: Primary human fibroblasts, mock-inoculated, 36h, bioreplicate 1


title: MFB001_icMERS_48h_RNA_A

source_name: Primary human fibroblasts, icMERS-inoculated, 48h, bioreplicate 1


title: MFB001_icMERS_48h_RNA_C

source_name: Primary human fibroblasts, icMERS-inoculated, 48h, bioreplicate 3


title: MFB001_Mock_36h_RNA_E

source_name: Primary human fibroblasts, mock-inoculated, 36h, bioreplicate 5


title: MFB001_icMERS_36h_RNA_E

source_name: Primary human fibroblasts, icMERS-inoculated, 36h, bioreplicate 5


title: MFB001_Mock_24h_RNA_A

source_name: Primary human fibroblasts, mock-inoculated, 24h, bioreplicate 1


title: MFB001_icMERS_48h_RNA_B

source_name: Primary human fibroblasts, icMERS-inoculated, 48h, bioreplicate 2


title: MFB001_Mock_24h_RNA_B

source_name: Primary human fibroblasts, mock-inoculated, 24h, bioreplicate 2


title: MFB001_Mock_24h_RNA_E

source_name: Primary human fibroblasts, mock-inoculated, 24h, bioreplicate 5


title: MFB001_icMERS_24h_RNA_D

source_name: Primary human fibroblasts, icMERS-inoculated, 24h, bioreplicate 4


title: MFB001_icMERS_24h_RNA_B

source_name: Primary human fibroblasts, icMERS-inoculated, 24h, bioreplicate 2


title: MFB001_icMERS_24h_RNA_A

source_name: Primary human fibroblasts, icMERS-inoculated, 24h, bioreplicate 1


title: MFB001_icMERS_24h_RNA_E

source_name: Primary human fibroblasts, icMERS-inoculated, 24h, bioreplicate 5


title: MFB001_Mock_12h_RNA_C

source_name: Primary human fibroblasts, mock-inoculated, 12h, bioreplicate 3


title: MFB001_icMERS_24h_RNA_C

source_name: Primary human fibroblasts, icMERS-inoculated, 24h, bioreplicate 3


title: MFB001_icMERS_12h_RNA_D

source_name: Primary human fibroblasts, icMERS-inoculated, 12h, bioreplicate 4


title: MFB001_Mock_12h_RNA_A

source_name: Primary human fibroblasts, mock-inoculated, 12h, bioreplicate 1


title: MFB001_icMERS_12h_RNA_E

source_name: Primary human fibroblasts, icMERS-inoculated, 12h, bioreplicate 5


title: MFB001_Mock_12h_RNA_D

source_name: Primary human fibroblasts, mock-inoculated, 12h, bioreplicate 4


title: MFB001_Mock_0h_RNA_E

source_name: Primary human fibroblasts, mock-inoculated, 0h, bioreplicate 5


title: MFB001_icMERS_12h_RNA_A

source_name: Primary human fibroblasts, icMERS-inoculated, 12h, bioreplicate 1


title: MFB001_icMERS_12h_RNA_B

source_name: Primary human fibroblasts, icMERS-inoculated, 12h, bioreplicate 2


title: MFB001_icMERS_12h_RNA_C

source_name: Primary human fibroblasts, icMERS-inoculated, 12h, bioreplicate 3


title: MFB001_Mock_12h_RNA_E

source_name: Primary human fibroblasts, mock-inoculated, 12h, bioreplicate 5


title: MFB001_icMERS_0h_RNA_E

source_name: Primary human fibroblasts, icMERS-inoculated, 0h, bioreplicate 5


title: MFB001_Mock_0h_RNA_D

source_name: Primary human fibroblasts, mock-inoculated, 0h, bioreplicate 4


title: MFB001_icMERS_0h_RNA_D

source_name: Primary human fibroblasts, icMERS-inoculated, 0h, bioreplicate 4


title: MMVE001_icMERS_24h_microRNA_5

status: Public on Apr 06 2016

source_name: Primary human microvascular endothelial cells, icMERS-inoculated, 24h, bioreplicate 5

treatment_protocol: MERSCOV002.0P: Cells were infected with a multiplicity of infection of 5 PFU.


title: MMVE001_Mock_24h_microRNA_1

source_name: Primary human microvascular endothelial cells, mock-inoculated, 24h, bioreplicate 1


title: EHUH001_mock_8h_1_microRNA

description: microRNA

source_name: Huh cells, mock-inoculated, 8h, bioreplicate 1


title: MFB001_icMERS_0h_RNA_B

source_name: Primary human fibroblasts, icMERS-inoculated, 0h, bioreplicate 2


title: MFB001_Mock_12h_RNA_B

source_name: Primary human fibroblasts, mock-inoculated, 12h, bioreplicate 2


title: MMVE001_Mock_24h_microRNA_3

source_name: Primary human microvascular endothelial cells, mock-inoculated, 24h, bioreplicate 3


title: MMVE001_Mock_24h_microRNA_4

source_name: Primary human microvascular endothelial cells, mock-inoculated, 24h, bioreplicate 4


title: MMVE001_Mock_12h_microRNA_4

source_name: Primary human microvascular endothelial cells, mock-inoculated, 12h, bioreplicate 4


title: MMVE001_Mock_12h_microRNA_1

source_name: Primary human microvascular endothelial cells, mock-inoculated, 12h, bioreplicate 1


title: MFB001_Mock_0h_RNA_B

source_name: Primary human fibroblasts, mock-inoculated, 0h, bioreplicate 2


title: MMVE001_icMERS_12h_microRNA_3

source_name: Primary human microvascular endothelial cells, icMERS-inoculated, 12h, bioreplicate 3


title: MFB001_icMERS_0h_RNA_A

source_name: Primary human fibroblasts, icMERS-inoculated, 0h, bioreplicate 1


title: MMVE001_icMERS_12h_microRNA_5

source_name: Primary human microvascular endothelial cells, icMERS-inoculated, 12h, bioreplicate 5


title: MMVE001_icMERS_12h_microRNA_2

source_name: Primary human microvascular endothelial cells, icMERS-inoculated, 12h, bioreplicate 2


title: MMVE001_Mock_24h_microRNA_5

source_name: Primary human microvascular endothelial cells, mock-inoculated, 24h, bioreplicate 5


title: MFB001_Mock_0h_RNA_A

source_name: Primary human fibroblasts, mock-inoculated, 0h, bioreplicate 1


title: MFB001_icMERS_0h_RNA_C

source_name: Primary human fibroblasts, icMERS-inoculated, 0h, bioreplicate 3


title: MMVE001_Mock_12h_microRNA_5

source_name: Primary human microvascular endothelial cells, mock-inoculated, 12h, bioreplicate 5


title: MMVE001_icMERS_12h_microRNA_1

source_name: Primary human microvascular endothelial cells, icMERS-inoculated, 12h, bioreplicate 1


title: MMVE001_icMERS_24h_microRNA_4

source_name: Primary human microvascular endothelial cells, icMERS-inoculated, 24h, bioreplicate 4


title: MMVE001_icMERS_12h_microRNA_4

source_name: Primary human microvascular endothelial cells, icMERS-inoculated, 12h, bioreplicate 4


title: MFB001_Mock_0h_RNA_C

source_name: Primary human fibroblasts, mock-inoculated, 0h, bioreplicate 3


title: MMVE001_mock_48h_RNA_5

status: Public on Mar 25 2016

source_name: Primary human microvascular endothelial cells, mock-inoculated, 48h, bioreplicate 5


title: MMVE001_icMERS_48h_RNA_5

source_name: Primary human microvascular endothelial cells, icMERS-inoculated, 48h, bioreplicate 5


title: MMVE001_mock_48h_RNA_1

source_name: Primary human microvascular endothelial cells, mock-inoculated, 48h, bioreplicate 1


title: MMVE001_mock_48h_RNA_3

source_name: Primary human microvascular endothelial cells, mock-inoculated, 48h, bioreplicate 3


title: MMVE001_mock_48h_RNA_4

source_name: Primary human microvascular endothelial cells, mock-inoculated, 48h, bioreplicate 4


title: MMVE001_icMERS_48h_RNA_1

source_name: Primary human microvascular endothelial cells, icMERS-inoculated, 48h, bioreplicate 1


title: MMVE001_icMERS_48h_RNA_4

source_name: Primary human microvascular endothelial cells, icMERS-inoculated, 48h, bioreplicate 4


title: MMVE001_icMERS_36h_RNA_4

source_name: Primary human microvascular endothelial cells, icMERS-inoculated, 36h, bioreplicate 4


title: MMVE001_mock_36h_RNA_5

source_name: Primary human microvascular endothelial cells, mock-inoculated, 36h, bioreplicate 5


title: MMVE001_mock_36h_RNA_4

source_name: Primary human microvascular endothelial cells, mock-inoculated, 36h, bioreplicate 4


title: MMVE001_mock_24h_RNA_5

source_name: Primary human microvascular endothelial cells, mock-inoculated, 24h, bioreplicate 5


title: MMVE001_icMERS_36h_RNA_2

source_name: Primary human microvascular endothelial cells, icMERS-inoculated, 36h, bioreplicate 2


title: MMVE001_mock_36h_RNA_3

source_name: Primary human microvascular endothelial cells, mock-inoculated, 36h, bioreplicate 3


title: MMVE001_mock_24h_RNA_3

source_name: Primary human microvascular endothelial cells, mock-inoculated, 24h, bioreplicate 3


title: MMVE001_icMERS_36h_RNA_1

source_name: Primary human microvascular endothelial cells, icMERS-inoculated, 36h, bioreplicate 1


title: MMVE001_icMERS_48h_RNA_3

source_name: Primary human microvascular endothelial cells, icMERS-inoculated, 48h, bioreplicate 3


title: MMVE001_icMERS_48h_RNA_2

source_name: Primary human microvascular endothelial cells, icMERS-inoculated, 48h, bioreplicate 2


title: MMVE001_mock_24h_RNA_2

source_name: Primary human microvascular endothelial cells, mock-inoculated, 24h, bioreplicate 2


title: MMVE001_mock_36h_RNA_2

source_name: Primary human microvascular endothelial cells, mock-inoculated, 36h, bioreplicate 2


title: MMVE001_icMERS_36h_RNA_3

source_name: Primary human microvascular endothelial cells, icMERS-inoculated, 36h, bioreplicate 3


title: MMVE001_mock_36h_RNA_1

source_name: Primary human microvascular endothelial cells, mock-inoculated, 36h, bioreplicate 1


title: MMVE001_mock_24h_RNA_1

source_name: Primary human microvascular endothelial cells, mock-inoculated, 24h, bioreplicate 1


title: MMVE001_icMERS_36h_RNA_5

source_name: Primary human microvascular endothelial cells, icMERS-inoculated, 36h, bioreplicate 5


title: MMVE001_icMERS_24h_RNA_4

source_name: Primary human microvascular endothelial cells, icMERS-inoculated, 24h, bioreplicate 4


title: MMVE001_icMERS_24h_RNA_3

source_name: Primary human microvascular endothelial cells, icMERS-inoculated, 24h, bioreplicate 3


title: MMVE001_mock_24h_RNA_4

source_name: Primary human microvascular endothelial cells, mock-inoculated, 24h, bioreplicate 4


title: MMVE001_icMERS_24h_RNA_2

source_name: Primary human microvascular endothelial cells, icMERS-inoculated, 24h, bioreplicate 2


title: MMVE001_icMERS_24h_RNA_1

source_name: Primary human microvascular endothelial cells, icMERS-inoculated, 24h, bioreplicate 1


title: MMVE001_mock_12h_RNA_1

source_name: Primary human microvascular endothelial cells, mock-inoculated, 12h, bioreplicate 1


title: MFB001_Mock_36h_microRNA_A

source_name: Primary human fibroblasts, mock-inoculated, 36h, bioreplicate 1


title: MMVE001_mock_12h_RNA_2

source_name: Primary human microvascular endothelial cells, mock-inoculated, 12h, bioreplicate 2


title: MMVE001_icMERS_12h_RNA_4

source_name: Primary human microvascular endothelial cells, icMERS-inoculated, 12h, bioreplicate 4


title: MMVE001_mock_12h_RNA_3

source_name: Primary human microvascular endothelial cells, mock-inoculated, 12h, bioreplicate 3


title: MMVE001_mock_0h_RNA_4

source_name: Primary human microvascular endothelial cells, mock-inoculated, 0h, bioreplicate 4


title: MMVE001_icMERS_12h_RNA_1

source_name: Primary human microvascular endothelial cells, icMERS-inoculated, 12h, bioreplicate 1


title: MMVE001_mock_12h_RNA_5

source_name: Primary human microvascular endothelial cells, mock-inoculated, 12h, bioreplicate 5


title: MMVE001_mock_0h_RNA_5

source_name: Primary human microvascular endothelial cells, mock-inoculated, 0h, bioreplicate 5


title: MMVE001_icMERS_12h_RNA_5

source_name: Primary human microvascular endothelial cells, icMERS-inoculated, 12h, bioreplicate 5


title: MMVE001_icMERS_0h_RNA_2

source_name: Primary human microvascular endothelial cells, icMERS-inoculated, 0h, bioreplicate 2


title: MMVE001_mock_0h_RNA_1

source_name: Primary human microvascular endothelial cells, mock-inoculated, 0h, bioreplicate 1


title: MMVE001_icMERS_0h_RNA_3

source_name: Primary human microvascular endothelial cells, icMERS-inoculated, 0h, bioreplicate 3


title: MMVE001_icMERS_12h_RNA_2

source_name: Primary human microvascular endothelial cells, icMERS-inoculated, 12h, bioreplicate 2


title: MMVE001_icMERS_0h_RNA_4

source_name: Primary human microvascular endothelial cells, icMERS-inoculated, 0h, bioreplicate 4


title: MMVE001_icMERS_24h_RNA_5

source_name: Primary human microvascular endothelial cells, icMERS-inoculated, 24h, bioreplicate 5


title: MMVE001_mock_0h_RNA_2

source_name: Primary human microvascular endothelial cells, mock-inoculated, 0h, bioreplicate 2


title: MMVE001_icMERS_0h_RNA_1

source_name: Primary human microvascular endothelial cells, icMERS-inoculated, 0h, bioreplicate 1


title: MMVE001_icMERS_0h_RNA_5

source_name: Primary human microvascular endothelial cells, icMERS-inoculated, 0h, bioreplicate 5


title: MMVE001_mock_0h_RNA_3

source_name: Primary human microvascular endothelial cells, mock-inoculated, 0h, bioreplicate 3


title: MMVE001_mock_12h_RNA_4

source_name: Primary human microvascular endothelial cells, mock-inoculated, 12h, bioreplicate 4


title: WLN002_WNVE218A_2d_microRNA_5

status: Public on Mar 19 2016

organism: Mus musculus

source_name: Lymph node, WNVE218A-inoculated, 2d, bioreplicate 5

growth_protocol: WNV001.0P: Culturing of cells for WNV propagation and quantification. WNV002.0P: Generation of WNV stocks.

extract_protocol: WNV006.0P: Harvesting protocol for transcriptomics of mouse tissue.

treatment_protocol: Mice are inoculated with WNV in both hind footpads. Draining lymph nodes (DLN) are collected from both hind legs for a total of 2 DLN. For mock inoculated mice, DLN from two mice are pooled for a total of 4 DLN. Infectivity is measured in paired serum samples from each WNV infected mouse.

tag: strain

tag: age

value: 5 weeks

tag: infection

tag: tissue

value: lymph node


title: WLN002_WNVE218A_2d_microRNA_3

source_name: Lymph node, WNVE218A-inoculated, 2d, bioreplicate 3


title: WLN002_WNVE218A_2d_microRNA_4

source_name: Lymph node, WNVE218A-inoculated, 2d, bioreplicate 4


title: WLN002_WNVE218A_1d_microRNA_5

source_name: Lymph node, WNVE218A-inoculated, 1d, bioreplicate 5


title: WLN002_WNVE218A_2d_microRNA_1

source_name: Lymph node, WNVE218A-inoculated, 2d, bioreplicate 1


title: WLN002_WNVE218A_1d_microRNA_4

source_name: Lymph node, WNVE218A-inoculated, 1d, bioreplicate 4


title: WLN002_WNVWT_2d_microRNA_5

source_name: Lymph node, WNVWT-inoculated, 2d, bioreplicate 5

value: WNVWT


title: WLN002_WNVE218A_1d_microRNA_1

source_name: Lymph node, WNVE218A-inoculated, 1d, bioreplicate 1


title: MMVE001_icMERS_12h_RNA_3

source_name: Primary human microvascular endothelial cells, icMERS-inoculated, 12h, bioreplicate 3


title: WLN002_WNVE218A_2d_microRNA_2

source_name: Lymph node, WNVE218A-inoculated, 2d, bioreplicate 2


title: WLN002_WNVE218A_1d_microRNA_3

source_name: Lymph node, WNVE218A-inoculated, 1d, bioreplicate 3


title: WLN002_WNVWT_2d_microRNA_4

source_name: Lymph node, WNVWT-inoculated, 2d, bioreplicate 4


title: WLN002_WNVWT_2d_microRNA_2

source_name: Lymph node, WNVWT-inoculated, 2d, bioreplicate 2


title: WLN002_WNVWT_2d_microRNA_1

source_name: Lymph node, WNVWT-inoculated, 2d, bioreplicate 1


title: WLN002_WNVWT_1d_microRNA_5

source_name: Lymph node, WNVWT-inoculated, 1d, bioreplicate 5


title: WLN002_WNVE218A_1d_microRNA_2

source_name: Lymph node, WNVE218A-inoculated, 1d, bioreplicate 2


title: WLN002_WNVWT_1d_microRNA_4

source_name: Lymph node, WNVWT-inoculated, 1d, bioreplicate 4


title: WLN002_WNVWT_2d_microRNA_3

source_name: Lymph node, WNVWT-inoculated, 2d, bioreplicate 3


title: WLN002_mock_2d_microRNA_3+4

source_name: Lymph node, mock-inoculated, 2d, bioreplicate 3+4


title: WLN002_mock_1d_microRNA_5+6

source_name: Lymph node, mock-inoculated, 1d, bioreplicate 5+6


title: WLN002_mock_1d_microRNA_3+4

source_name: Lymph node, mock-inoculated, 1d, bioreplicate 3+4


title: WLN002_WNVWT_1d_microRNA_2

source_name: Lymph node, WNVWT-inoculated, 1d, bioreplicate 2


title: WLN002_mock_2d_microRNA_1+2

source_name: Lymph node, mock-inoculated, 2d, bioreplicate 1+2


title: WLN002_WNVWT_1d_microRNA_1

source_name: Lymph node, WNVWT-inoculated, 1d, bioreplicate 1


title: WLN002_mock_1d_microRNA_1+2

source_name: Lymph node, mock-inoculated, 1d, bioreplicate 1+2


title: WLN002_WNVWT_1d_microRNA_3

source_name: Lymph node, WNVWT-inoculated, 1d, bioreplicate 3


title: WLN002_mock_2d_microRNA_5+6

source_name: Lymph node, mock-inoculated, 2d, bioreplicate 5+6


title: WLN002_WNVE218A_2d_RNA_5

status: Public on Mar 15 2016

source_name: Lymph node, WNVE218A-inoculated, 2d, bioreplicate 5


title: WLN002_WNVE218A_4d_RNA_3

source_name: Lymph node, WNVE218A-inoculated, 4d, bioreplicate 3


title: WLN002_WNVE218A_6d_RNA_2

source_name: Lymph node, WNVE218A-inoculated, 6d, bioreplicate 2


title: WLN002_WNVE218A_4d_RNA_4

source_name: Lymph node, WNVE218A-inoculated, 4d, bioreplicate 4


title: WLN002_WNVE218A_1d_RNA_5

source_name: Lymph node, WNVE218A-inoculated, 1d, bioreplicate 5


title: WLN002_WNVE218A_2d_RNA_2

source_name: Lymph node, WNVE218A-inoculated, 2d, bioreplicate 2


title: WLN002_WNVE218A_4d_RNA_1

source_name: Lymph node, WNVE218A-inoculated, 4d, bioreplicate 1


title: WLN002_WNVE218A_6d_RNA_5

source_name: Lymph node, WNVE218A-inoculated, 6d, bioreplicate 5


title: MDC001_icMERS_24h_RNA_3

source_name: Primary human dendritic cells, icMERS-inoculated, 24h, bioreplicate 3

growth_protocol: Cells were harvested by a group at UNC, picked up by this group, and infected. There was no real growth for this set of cells.

extract_protocol: MERSCOV001.0P: Harvesting protocol for panomics of mDC

treatment_protocol: MERSCOV001.0P: Cells were infected with a multiplicity of infection of 5 PFU.

tag: biological replicate

value: dendritic cells


title: WLN002_WNVE218A_6d_RNA_4

source_name: Lymph node, WNVE218A-inoculated, 6d, bioreplicate 4


title: WLN002_WNVE218A_4d_RNA_2

source_name: Lymph node, WNVE218A-inoculated, 4d, bioreplicate 2


title: WLN002_WNVE218A_2d_RNA_3

source_name: Lymph node, WNVE218A-inoculated, 2d, bioreplicate 3


title: WLN002_WNVE218A_2d_RNA_4

source_name: Lymph node, WNVE218A-inoculated, 2d, bioreplicate 4


title: WLN002_WNVE218A_4d_RNA_5

source_name: Lymph node, WNVE218A-inoculated, 4d, bioreplicate 5


title: MDC001_icMERS_24h_RNA_1

source_name: Primary human dendritic cells, icMERS-inoculated, 24h, bioreplicate 1


title: WLN002_WNVE218A_1d_RNA_4

source_name: Lymph node, WNVE218A-inoculated, 1d, bioreplicate 4


title: WLN002_WNVE218A_1d_RNA_2

source_name: Lymph node, WNVE218A-inoculated, 1d, bioreplicate 2


title: MDC001_icMERS_24h_RNA_2

source_name: Primary human dendritic cells, icMERS-inoculated, 24h, bioreplicate 2


title: WLN002_WNVE218A_2d_RNA_1

source_name: Lymph node, WNVE218A-inoculated, 2d, bioreplicate 1


title: WLN002_WNVWT_6d_RNA_5

source_name: Lymph node, WNVWT-inoculated, 6d, bioreplicate 5


title: WLN002_WNVE218A_1d_RNA_3

source_name: Lymph node, WNVE218A-inoculated, 1d, bioreplicate 3


title: WLN002_WNVE218A_6d_RNA_1

source_name: Lymph node, WNVE218A-inoculated, 6d, bioreplicate 1


title: WLN002_WNVWT_6d_RNA_4

source_name: Lymph node, WNVWT-inoculated, 6d, bioreplicate 4


title: WLN002_WNVE218A_6d_RNA_3

source_name: Lymph node, WNVE218A-inoculated, 6d, bioreplicate 3


title: MDC001_icMERS_12h_RNA_1

source_name: Primary human dendritic cells, icMERS-inoculated, 12h, bioreplicate 1


title: WLN002_WNVE218A_1d_RNA_1

source_name: Lymph node, WNVE218A-inoculated, 1d, bioreplicate 1


title: MDC001_icMERS_12h_RNA_3

source_name: Primary human dendritic cells, icMERS-inoculated, 12h, bioreplicate 3


title: MDC001_icMERS_12h_RNA_2

source_name: Primary human dendritic cells, icMERS-inoculated, 12h, bioreplicate 2


title: MDC001_icMERS_7h_RNA_1

source_name: Primary human dendritic cells, icMERS-inoculated, 7h, bioreplicate 1


title: MDC001_icMERS_7h_RNA_2

source_name: Primary human dendritic cells, icMERS-inoculated, 7h, bioreplicate 2


title: WLN002_WNVWT_6d_RNA_3

source_name: Lymph node, WNVWT-inoculated, 6d, bioreplicate 3


title: WLN002_WNVWT_6d_RNA_2

source_name: Lymph node, WNVWT-inoculated, 6d, bioreplicate 2


title: MDC001_icMERS_7h_RNA_3

source_name: Primary human dendritic cells, icMERS-inoculated, 7h, bioreplicate 3


title: MDC001_mock_12h_RNA_3

source_name: Primary human dendritic cells, mock-inoculated, 12h, bioreplicate 3


title: WLN002_WNVWT_4d_RNA_5

source_name: Lymph node, WNVWT-inoculated, 4d, bioreplicate 5


title: MDC001_icMERS_3h_RNA_1

source_name: Primary human dendritic cells, icMERS-inoculated, 3h, bioreplicate 1


title: WLN002_WNVWT_2d_RNA_2

source_name: Lymph node, WNVWT-inoculated, 2d, bioreplicate 2


title: MDC001_icMERS_3h_RNA_2

source_name: Primary human dendritic cells, icMERS-inoculated, 3h, bioreplicate 2


title: MDC001_icMERS_0h_RNA_3

source_name: Primary human dendritic cells, icMERS-inoculated, 0h, bioreplicate 3


title: MDC001_icMERS_3h_RNA_3

source_name: Primary human dendritic cells, icMERS-inoculated, 3h, bioreplicate 3


title: WLN002_WNVWT_4d_RNA_2

source_name: Lymph node, WNVWT-inoculated, 4d, bioreplicate 2


title: WLN002_WNVWT_2d_RNA_4

source_name: Lymph node, WNVWT-inoculated, 2d, bioreplicate 4


title: WLN002_WNVWT_2d_RNA_3

source_name: Lymph node, WNVWT-inoculated, 2d, bioreplicate 3


title: WLN002_WNVWT_4d_RNA_1

source_name: Lymph node, WNVWT-inoculated, 4d, bioreplicate 1


title: MDC001_mock_12h_RNA_1

source_name: Primary human dendritic cells, mock-inoculated, 12h, bioreplicate 1


title: MDC001_mock_24h_RNA_3

source_name: Primary human dendritic cells, mock-inoculated, 24h, bioreplicate 3


title: MDC001_icMERS_0h_RNA_1

source_name: Primary human dendritic cells, icMERS-inoculated, 0h, bioreplicate 1


title: MDC001_icMERS_0h_RNA_2

source_name: Primary human dendritic cells, icMERS-inoculated, 0h, bioreplicate 2


title: MDC001_mock_24h_RNA_2

source_name: Primary human dendritic cells, mock-inoculated, 24h, bioreplicate 2


title: WLN002_WNVWT_2d_RNA_1

source_name: Lymph node, WNVWT-inoculated, 2d, bioreplicate 1


title: MDC001_mock_24h_RNA_1

source_name: Primary human dendritic cells, mock-inoculated, 24h, bioreplicate 1


title: WLN002_WNVWT_1d_RNA_5

source_name: Lymph node, WNVWT-inoculated, 1d, bioreplicate 5


title: WLN002_WNVWT_4d_RNA_4

source_name: Lymph node, WNVWT-inoculated, 4d, bioreplicate 4


title: WLN002_WNVWT_2d_RNA_5

source_name: Lymph node, WNVWT-inoculated, 2d, bioreplicate 5


title: WLN002_WNVWT_6d_RNA_1

source_name: Lymph node, WNVWT-inoculated, 6d, bioreplicate 1


title: MDC001_mock_12h_RNA_2

source_name: Primary human dendritic cells, mock-inoculated, 12h, bioreplicate 2


title: WLN002_WNVWT_1d_RNA_4

source_name: Lymph node, WNVWT-inoculated, 1d, bioreplicate 4


title: MDC001_mock_7h_RNA_3

source_name: Primary human dendritic cells, mock-inoculated, 7h, bioreplicate 3


title: WLN002_WNVWT_1d_RNA_3

source_name: Lymph node, WNVWT-inoculated, 1d, bioreplicate 3


title: MDC001_mock_7h_RNA_1

source_name: Primary human dendritic cells, mock-inoculated, 7h, bioreplicate 1


title: MDC001_mock_3h_RNA_3

source_name: Primary human dendritic cells, mock-inoculated, 3h, bioreplicate 3


title: WLN002_WNVWT_4d_RNA_3

source_name: Lymph node, WNVWT-inoculated, 4d, bioreplicate 3


title: MDC001_mock_7h_RNA_2

source_name: Primary human dendritic cells, mock-inoculated, 7h, bioreplicate 2


title: MDC001_mock_3h_RNA_2

source_name: Primary human dendritic cells, mock-inoculated, 3h, bioreplicate 2


title: WLN002_mock_6d_RNA_3+4

source_name: Lymph node, mock-inoculated, 6d, bioreplicate 3+4


title: WLN002_mock_6d_RNA_1+2

source_name: Lymph node, mock-inoculated, 6d, bioreplicate 1+2


title: MDC001_mock_0h_RNA_3

source_name: Primary human dendritic cells, mock-inoculated, 0h, bioreplicate 3


title: WLN002_mock_4d_RNA_5+6

source_name: Lymph node, mock-inoculated, 4d, bioreplicate 5+6


title: MDC001_mock_0h_RNA_2

source_name: Primary human dendritic cells, mock-inoculated, 0h, bioreplicate 2


title: WLN002_WNVWT_1d_RNA_1

source_name: Lymph node, WNVWT-inoculated, 1d, bioreplicate 1


title: WLN002_mock_4d_RNA_3+4

source_name: Lymph node, mock-inoculated, 4d, bioreplicate 3+4


title: WLN002_mock_2d_RNA_5+6

source_name: Lymph node, mock-inoculated, 2d, bioreplicate 5+6


title: MDC001_mock_0h_RNA_1

source_name: Primary human dendritic cells, mock-inoculated, 0h, bioreplicate 1


title: WCT001_WNVE218A_4d_microRNA_3

status: Public on Mar 12 2016

source_name: Cerebellum, WNVWE218A-inoculated, 4d, bioreplicate 3

treatment_protocol: Tissues were harvested from naïve or WNV inoculated 5 week old C57BL6/J mice. Mice were inoculated with a dose of 100 FFU of WNV intracranially.

tag: time (day)


title: WCT001_WNVE218A_4d_microRNA_4

source_name: Cerebellum, WNVWE218A-inoculated, 4d, bioreplicate 4


title: WCT001_WNVE218A_4d_microRNA_2

source_name: Cerebellum, WNVWE218A-inoculated, 4d, bioreplicate 2


title: WCT001_WNVE218A_4d_microRNA_5

source_name: Cerebellum, WNVWE218A-inoculated, 4d, bioreplicate 5


title: WLN002_WNVWT_1d_RNA_2

source_name: Lymph node, WNVWT-inoculated, 1d, bioreplicate 2


title: WLN002_mock_2d_RNA_1+2

source_name: Lymph node, mock-inoculated, 2d, bioreplicate 1+2


title: WLN002_mock_1d_RNA_5+6

source_name: Lymph node, mock-inoculated, 1d, bioreplicate 5+6


title: WLN002_mock_2d_RNA_3+4

source_name: Lymph node, mock-inoculated, 2d, bioreplicate 3+4


title: WCT001_WNVE218A_4d_microRNA_1

source_name: Cerebellum, WNVWE218A-inoculated, 4d, bioreplicate 1


title: WCT001_WNVE218A_2d_microRNA_1

source_name: Cerebellum, WNVWE218A-inoculated, 2d, bioreplicate 1


title: WCT001_WNVE218A_2d_microRNA_5

source_name: Cerebellum, WNVWE218A-inoculated, 2d, bioreplicate 5


title: WCT001_WNVE218A_2d_microRNA_2

source_name: Cerebellum, WNVWE218A-inoculated, 2d, bioreplicate 2


title: WLN002_mock_1d_RNA_3+4

source_name: Lymph node, mock-inoculated, 1d, bioreplicate 3+4


title: WCT001_WNVE218A_2d_microRNA_4

source_name: Cerebellum, WNVWE218A-inoculated, 2d, bioreplicate 4


title: WCT001_WNVE218A_2d_microRNA_3

source_name: Cerebellum, WNVWE218A-inoculated, 2d, bioreplicate 3


title: WLN002_mock_4d_RNA_1+2

source_name: Lymph node, mock-inoculated, 4d, bioreplicate 1+2


title: WLN002_mock_1d_RNA_1+2

source_name: Lymph node, mock-inoculated, 1d, bioreplicate 1+2


title: WCT001_WNVWT_4d_microRNA_5

source_name: Cerebellum, WNVWT-inoculated, 4d, bioreplicate 5


title: WCT001_WNVWT_4d_microRNA_1

source_name: Cerebellum, WNVWT-inoculated, 4d, bioreplicate 1


title: WCT001_WNVWT_2d_microRNA_5

source_name: Cerebellum, WNVWT-inoculated, 2d, bioreplicate 5


title: WCT001_WNVWT_4d_microRNA_4

source_name: Cerebellum, WNVWT-inoculated, 4d, bioreplicate 4


title: WCT001_WNVWT_2d_microRNA_4

source_name: Cerebellum, WNVWT-inoculated, 2d, bioreplicate 4


title: WCT001_WNVWT_2d_microRNA_3

source_name: Cerebellum, WNVWT-inoculated, 2d, bioreplicate 3


title: WCT001_WNVWT_4d_microRNA_3

source_name: Cerebellum, WNVWT-inoculated, 4d, bioreplicate 3


title: WCT001_WNVWT_2d_microRNA_1

source_name: Cerebellum, WNVWT-inoculated, 2d, bioreplicate 1


title: WCT001_mock_2d_microRNA_5

source_name: Cerebellum, mock-inoculated, 2d, bioreplicate 5


title: WCT001_mock_4d_microRNA_5

source_name: Cerebellum, mock-inoculated, 4d, bioreplicate 5


title: WCT001_mock_4d_microRNA_4

source_name: Cerebellum, mock-inoculated, 4d, bioreplicate 4


title: WCT001_mock_4d_microRNA_3

source_name: Cerebellum, mock-inoculated, 4d, bioreplicate 3


title: WCT001_WNVWT_2d_microRNA_2

source_name: Cerebellum, WNVWT-inoculated, 2d, bioreplicate 2


title: WCT001_WNVWT_4d_microRNA_2

source_name: Cerebellum, WNVWT-inoculated, 4d, bioreplicate 2


title: WCT001_mock_4d_microRNA_1

source_name: Cerebellum, mock-inoculated, 4d, bioreplicate 1


title: WCT001_mock_2d_microRNA_2

source_name: Cerebellum, mock-inoculated, 2d, bioreplicate 2


title: WCT001_mock_2d_microRNA_4

source_name: Cerebellum, mock-inoculated, 2d, bioreplicate 4


title: WCT001_mock_2d_microRNA_1

source_name: Cerebellum, mock-inoculated, 2d, bioreplicate 1


title: WCT001_mock_2d_microRNA_3

source_name: Cerebellum, mock-inoculated, 2d, bioreplicate 3


title: WCT001_mock_4d_microRNA_2

source_name: Cerebellum, mock-inoculated, 4d, bioreplicate 2


title: WCT001_WNVE218A_2d_RNA_5

status: Public on Mar 04 2016

source_name: Cortex, WNVE218A-inoculated, 2d, bioreplicate 5


title: WCT001_WNVE218A_6d_RNA_3

source_name: Cortex, WNVE218A-inoculated, 6d, bioreplicate 3


title: WCT001_WNVE218A_4d_RNA_5

source_name: Cortex, WNVE218A-inoculated, 4d, bioreplicate 5


title: WCT001_WNVE218A_4d_RNA_2

source_name: Cortex, WNVE218A-inoculated, 4d, bioreplicate 2


title: WCT001_WNVE218A_2d_RNA_4

source_name: Cortex, WNVE218A-inoculated, 2d, bioreplicate 4


title: WCT001_WNVE218A_4d_RNA_3

source_name: Cortex, WNVE218A-inoculated, 4d, bioreplicate 3


title: WCT001_WNVE218A_2d_RNA_1

source_name: Cortex, WNVE218A-inoculated, 2d, bioreplicate 1


title: WCT001_WNVE218A_6d_RNA_2

source_name: Cortex, WNVE218A-inoculated, 6d, bioreplicate 2


title: WCT001_WNVE218A_6d_RNA_5

source_name: Cortex, WNVE218A-inoculated, 6d, bioreplicate 5


title: WCT001_WNVE218A_4d_RNA_1

source_name: Cortex, WNVE218A-inoculated, 4d, bioreplicate 1


title: WCT001_WNVE218A_4d_RNA_4

source_name: Cortex, WNVE218A-inoculated, 4d, bioreplicate 4


title: WCT001_WNVE218A_1d_RNA_3

source_name: Cortex, WNVE218A-inoculated, 1d, bioreplicate 3


title: WCT001_WNVWT_6d_RNA_5

source_name: Cortex, WNVWT-inoculated, 6d, bioreplicate 5


title: WCT001_WNVE218A_2d_RNA_3

source_name: Cortex, WNVE218A-inoculated, 2d, bioreplicate 3


title: WCT001_WNVE218A_2d_RNA_2

source_name: Cortex, WNVE218A-inoculated, 2d, bioreplicate 2


title: WCT001_WNVE218A_6d_RNA_1

source_name: Cortex, WNVE218A-inoculated, 6d, bioreplicate 1


title: WCT001_WNVWT_6d_RNA_1

source_name: Cortex, WNVWT-inoculated, 6d, bioreplicate 1


title: WCT001_WNVWT_6d_RNA_4

source_name: Cortex, WNVWT-inoculated, 6d, bioreplicate 4


title: WCT001_WNVWT_4d_RNA_4

source_name: Cortex, WNVWT-inoculated, 4d, bioreplicate 4


title: WCT001_WNVWT_6d_RNA_2

source_name: Cortex, WNVWT-inoculated, 6d, bioreplicate 2


title: WCT001_WNVE218A_6d_RNA_4

source_name: Cortex, WNVE218A-inoculated, 6d, bioreplicate 4


title: WCT001_WNVWT_4d_RNA_3

source_name: Cortex, WNVWT-inoculated, 4d, bioreplicate 3


title: WCT001_WNVWT_4d_RNA_1

source_name: Cortex, WNVWT-inoculated, 4d, bioreplicate 1


title: WCT001_WNVE218A_1d_RNA_4

source_name: Cortex, WNVE218A-inoculated, 1d, bioreplicate 4


title: WCT001_WNVE218A_1d_RNA_5

source_name: Cortex, WNVE218A-inoculated, 1d, bioreplicate 5


title: WCT001_WNVWT_4d_RNA_5

source_name: Cortex, WNVWT-inoculated, 4d, bioreplicate 5


title: WCT001_WNVWT_6d_RNA_3

source_name: Cortex, WNVWT-inoculated, 6d, bioreplicate 3


title: WCT001_WNVE218A_1d_RNA_1

source_name: Cortex, WNVE218A-inoculated, 1d, bioreplicate 1


title: WCT001_WNVWT_2d_RNA_5

source_name: Cortex, WNVWT-inoculated, 2d, bioreplicate 5


title: WCT001_WNVWT_4d_RNA_2

source_name: Cortex, WNVWT-inoculated, 4d, bioreplicate 2


title: WCT001_WNVWT_2d_RNA_3

source_name: Cortex, WNVWT-inoculated, 2d, bioreplicate 3


title: WCT001_WNVWT_2d_RNA_4

source_name: Cortex, WNVWT-inoculated, 2d, bioreplicate 4


title: WCT001_WNVWT_2d_RNA_1

source_name: Cortex, WNVWT-inoculated, 2d, bioreplicate 1


title: WCT001_WNVWT_1d_RNA_4

source_name: Cortex, WNVWT-inoculated, 1d, bioreplicate 4


title: WCT001_WNVWT_1d_RNA_2

source_name: Cortex, WNVWT-inoculated, 1d, bioreplicate 2


title: WCT001_WNVWT_1d_RNA_3

source_name: Cortex, WNVWT-inoculated, 1d, bioreplicate 3


title: WCT001_WNVWT_1d_RNA_5

source_name: Cortex, WNVWT-inoculated, 1d, bioreplicate 5


title: WCT001_mock_4d_RNA_3

source_name: Cortex, mock-inoculated, 4d, bioreplicate 3


title: WCT001_mock_6d_RNA_4

source_name: Cortex, mock-inoculated, 6d, bioreplicate 4


title: WCT001_mock_6d_RNA_3

source_name: Cortex, mock-inoculated, 6d, bioreplicate 3


title: WCT001_WNVWT_1d_RNA_1

source_name: Cortex, WNVWT-inoculated, 1d, bioreplicate 1


title: WCT001_mock_6d_RNA_1

source_name: Cortex, mock-inoculated, 6d, bioreplicate 1


title: WCT001_mock_4d_RNA_2

source_name: Cortex, mock-inoculated, 4d, bioreplicate 2


title: WCT001_mock_6d_RNA_2

source_name: Cortex, mock-inoculated, 6d, bioreplicate 2


title: WCT001_WNVE218A_1d_RNA_2

source_name: Cortex, WNVE218A-inoculated, 1d, bioreplicate 2


title: WCT001_mock_4d_RNA_4

source_name: Cortex, mock-inoculated, 4d, bioreplicate 4


title: WCT001_mock_6d_RNA_5

source_name: Cortex, mock-inoculated, 6d, bioreplicate 5


title: WCT001_mock_4d_RNA_5

source_name: Cortex, mock-inoculated, 4d, bioreplicate 5


title: WCT001_WNVWT_2d_RNA_2

source_name: Cortex, WNVWT-inoculated, 2d, bioreplicate 2


title: WCT001_mock_2d_RNA_4

source_name: Cortex, mock-inoculated, 2d, bioreplicate 4


title: WCT001_mock_1d_RNA_1

source_name: Cortex, mock-inoculated, 1d, bioreplicate 1


title: WCT001_mock_1d_RNA_4

source_name: Cortex, mock-inoculated, 1d, bioreplicate 4


title: WCT001_mock_4d_RNA_1

source_name: Cortex, mock-inoculated, 4d, bioreplicate 1


title: WCT001_mock_2d_RNA_1

source_name: Cortex, mock-inoculated, 2d, bioreplicate 1


title: WCT001_mock_2d_RNA_2

source_name: Cortex, mock-inoculated, 2d, bioreplicate 2


title: WCT001_mock_2d_RNA_3

source_name: Cortex, mock-inoculated, 2d, bioreplicate 3


title: DZ12_lowgal_33 (DZ12-33)

status: Public on Mar 03 2016

type: genomic

city: Hangzhou

first: Daoqiong

last: Zheng

state: Zhejiang

address: 866 Yuhangtang Road

department: Institute of Microbiology

country: China

institute: College of Life Sciences, Zhejiang University

description: DZ12-derived isolates from one cycle growth on low-gal medium

hyb_protocol: The hybridization reactions were prepared using an Agilent Oligo aCGH/ChIP-on-Chip Hybridization kit (5188-5220) following kit instructions. Arrays were incubated for 24 hours at 62°. Following hybridization, the arrays were washed for 5 minutes in Oligo aCGH/ChIP-on-Chip Wash Buffer 1 (Agilent 5188-5221) and 1 minute in Oligo aCGH/ChIP-on-Chip Wash Buffer 2 (Agilent 5188-5222) that was pre-warmed to 37°. The arrays were then scanned at wavelengths of 635 and 532 nm using the GenePix scanner and the GenePix Pro software using settings recommended by the manufacturer.Microarrays could be re-used approximately 4-6 times by removing the hybridized labeled DNA sequences from the oligonucleotides. Microarrays and gasket slides were stripped separately in 1x stripping buffer (10 mM potassium phosphate, pH6.6). The slides were slowly heated to the boiling point in the stripping buffer for 30-45 minutes. After stripping, they were transferred to deionized water, and then slowly removed and stored in a nitrogen cabinet. The gasket slides were centrifuged at 500 rpm to remove excess liquid. Labels on microarrays were removed prior to stripping.

scan_protocol: The data generated by GenePix Pro were exported as .gpr files and analyzed with a homemade software pipeline. Probes that were flagged by the software were deleted from the analysis.

data_processing: The ratio of the medians (635 nm/532 nm) for each probe was used for analysis, and replicate probe medians were averaged. The data was centered around one by dividing each probe median by the average of all of the probe medians in order to normalize for differences in the hybridization levels for the reference and experimental strain samples. The data were plotted separately for each haploid parental strain and, in plots showing whole chromosomes, the medians were “smoothed” by averaging over nine consecutive probes. In the labels of the oligonucleotides “SF”, “SR”, “YF” and “YR” refer to the orientation and origin of the sequences used for the oligonucleotides on the microarrays. “S” and “Y” indicate whether the oligonucleotide contains sequences identical to the reference S288c genome (closely related to W303-1A) or the YJM789 genome, respectively. “F” and “R” indicate whether the orientation (5’ to 3’) is identical to the orientation in SGD (“F” standing for forward) or opposite to that shown in SGD (“R” for reverse). Thus, “F” and “R” have the sequences of the complementary Watson and Crick strands at each position. For all oligonucleotides for a particular SNP, the number used to label the coordinate is the coordinate of the first base of the oligonucleotide in the “F” orientation. In addition, the oligonucleotides for the YJM789-derived sequences were labeled with the same coordinate as the S288c-derived sequences. The sequence information for the YJM789 sequences was from SGD based on the paper by (Wei et al., 2007. PNAS 104:12825-12830). The Whole genome arrays were completed with Agilent platform with Design ID Agilent-027438 was used. Sectored colonies were mapped using Agilent platforsm with Design IDs Agilent-031671, which contains probes specific only to chromosome IV, and Agilent-047217, which contains many probes on chromsome IV, but few probes near the telomere and centromere of all chromosomes.

molecule: genomic DNA

organism: Saccharomyces cerevisiae

source_name: DZ12-derived isolates

label_protocol: The description of the labeling and hybridization protocol are copied with minor modifications from the Supplementary Information of our previous publication: DNA from these colonies was labeled with Cy5, and mixed with control DNA (DNA from the strain JSC24) labeled with Cy3. The two samples were mixed and competitively hybridized to the SNP microarrays.

growth_protocol: Yeast cells were cultured in low-gal (2% yeast extract, 2% peptone and 3% raffinose, 0.005% galactose) or high-gal medium (2% yeast extract, 2% peptone and 3% raffinose, 0.05% galactose) to extract DNA

extract_protocol: Genomic DNA were prepared DNA by standard procedures (St. Charles et al., 2012).

treatment_protocol: The expression of POL3 were downregulated using either low-gal medium or YPD medium.

tag: ploidy

value: Diploid

tag: background

value: diploid strain DZ12

tag: growth protocol

value: one cycle of growth on low-gal medium

source_name: Control strain JSC24

value: control strain JSC24


title: WCT001_mock_1d_RNA_5

source_name: Cortex, mock-inoculated, 1d, bioreplicate 5


title: DZ12_lowgal_35 (DZ12-35)


title: WCT001_mock_1d_RNA_2

source_name: Cortex, mock-inoculated, 1d, bioreplicate 2


title: DZ12_lowgal_34 (DZ12-34)


title: WCT001_mock_1d_RNA_3

source_name: Cortex, mock-inoculated, 1d, bioreplicate 3


title: DZ12_lowgal_30 (DZ12-30)


title: DZ12_lowgal_32 (DZ12-32)


title: DZ12_lowgal_28 (DZ12-28)


title: DZ12_lowgal_24 (DZ12-24)


title: WCT001_mock_2d_RNA_5

source_name: Cortex, mock-inoculated, 2d, bioreplicate 5


title: DZ12_lowgal_29 (DZ12-29)


title: DZ12_lowgal_18 (DZ18-18)


title: DZ12_lowgal_27 (DZ12-27)


title: DZ12_lowgal_19 (DZ12-19)


title: DZ12_lowgal_25 (DZ12-25)


title: DZ12_lowgal_21 (DZ12-21)


title: DZ12_lowgal_15 (DZ12-15)


title: DZ12_lowgal_14 (DZ12-14)


title: DZ12_lowgal_17 (DZ12-17)


title: DZ12_lowgal_23 (DZ12-23)


title: DZ12_lowgal_16 (DZ12-16)


title: DZ12_lowgal_26 (DZ12-26)


title: DZ12_lowgal_12 (DZ12-12)


title: DZ12_lowgal_20 (DZ12-20)


title: DZ12_lowgal_22 (DZ12-22)


title: DZ12_lowgal_9 (DZ12-9)


title: DZ12_lowgal_11 (DZ12-11)


title: DZ12_lowgal_31 (DZ12-31)


title: DZ12_lowgal_8 (DZ12-8)


title: DZ12_lowgal_10 (DZ12-10)


title: DZ12_lowgal_7 (DZ12-7)


title: DZ12_lowgal_4 (DZ12-4)


title: DZ12_lowgal_6 (DZ12-6)


title: DZ12_lowgal_13 (DZ12-13)


title: DZ12_lowgal_1 (DZ12-1)


title: DZ12_lowgal_3 (DZ12-3)


title: sample 17: P53+WDR77 siRNA, LNCaP cells, vehicle treated 2

status: Public on Mar 01 2016

city: Buffalo

first: Qiang

last: Hu

state: NY

address: Elm & Carlton Streets

institute: Roswell Park Cancer Institute

description: sample 17

hyb_protocol: The labeled probes were then mixed with hybridization reagents and hybridized overnight to the HumanHT12 v4 BeadChips.

scan_protocol: Following washing and staining, the BeadChips were imaged using the Illumina BeadArray Reader to measure fluorescence intensity at each probe.

data_processing: BeadChip data files are analyzed with Illumina’s GenomeStudio gene expression module (V1.9.0)

label: Biotin

source_name: LNCaP cells, vehicle treated 2

label_protocol: Initially, we converted 250-500 ng total RNA to cDNA, followed by in vitro transcription to generate biotin-labeled cRNA (Illumina TotalPrep RNA Amplification Kit, Ambion Inc., Austin, TX).

extract_protocol: Total RNA was isolated using TRIZOL reagent (Invitrogen).

value: LNCaP

tag: transfected with

value: P53+WDR77 siRNA


title: sample 23: WDR77 siRNA, LNCaP cells, vehicle treated 2

description: sample 23

value: WDR77 siRNA


title: sample 22: control siRNA, LNCaP cells, R1881 treated 3

description: sample 22

source_name: LNCaP cells, R1881 treated 3

value: control siRNA


title: sample 15: p53 siRNA, LNCaP cells, vehicle treated 1

description: sample 15

source_name: LNCaP cells, vehicle treated 1

value: p53 siRNA


title: DZ12_lowgal_5 (DZ12-5)


title: sample 9: P53+WDR77 siRNA, LNCaP cells, vehicle treated 3

description: sample 9

source_name: LNCaP cells, vehicle treated 3


title: sample 10: WDR77 siRNA, LNCaP cells, R1881 treated 2

description: sample 10

source_name: LNCaP cells, R1881 treated 2


title: DZ12_lowgal_2 (DZ12-2)


title: sample 21: p53 siRNA, LNCaP cells, vehicle treated 3

description: sample 21


title: sample 20: control siRNA, LNCaP cells, vehicle treated 2

description: sample 20


title: WCB001_WNVE218A_4d_microRNA_5

status: Public on Feb 21 2016

source_name: Cerebellum, WNVWE218A-inoculated, 4d, bioreplicate 5


title: WCB001_WNVE218A_4d_microRNA_4

source_name: Cerebellum, WNVWE218A-inoculated, 4d, bioreplicate 4


title: sample 4: P53+WDR77 siRNA, LNCaP cells, R1881 treated 2

description: sample 4


title: sample 12: p53 siRNA, LNCaP cells, vehicle treated 2

description: sample 12


title: sample 16: P53+WDR77 siRNA, LNCaP cells, R1881 treated 3

description: sample 16


title: sample 8: control siRNA, LNCaP cells, R1881 treated 2

description: sample 8


title: sample 24: p53 siRNA, LNCaP cells, R1881 treated 2

description: sample 24


title: sample 18: P53+WDR77 siRNA, LNCaP cells, R1881 treated 1

description: sample 18

source_name: LNCaP cells, R1881 treated 1


title: WCB001_WNVE218A_2d_microRNA_3

source_name: Cerebellum, WNVWE218A-inoculated, 2d, bioreplicate 3


title: WCB001_WNVE218A_2d_microRNA_2

source_name: Cerebellum, WNVWE218A-inoculated, 2d, bioreplicate 2


title: WCB001_WNVE218A_2d_microRNA_1

source_name: Cerebellum, WNVWE218A-inoculated, 2d, bioreplicate 1


title: WCB001_WNVWT_4d_microRNA_3

source_name: Cerebellum, WNVWT-inoculated, 4d, bioreplicate 3


title: WCB001_WNVWT_4d_microRNA_4

source_name: Cerebellum, WNVWT-inoculated, 4d, bioreplicate 4


title: sample 5: control siRNA, LNCaP cells, vehicle treated 3

description: sample 5


title: WCB001_WNVE218A_2d_microRNA_5

source_name: Cerebellum, WNVWE218A-inoculated, 2d, bioreplicate 5


title: WCB001_WNVE218A_4d_microRNA_3

source_name: Cerebellum, WNVWE218A-inoculated, 4d, bioreplicate 3


title: sample 6: P53+WDR77 siRNA, LNCaP cells, vehicle treated 1

description: sample 6


title: sample 3: WDR77 siRNA, LNCaP cells, vehicle treated 1

description: sample 3


title: WCB001_WNVE218A_4d_microRNA_1

source_name: Cerebellum, WNVWE218A-inoculated, 4d, bioreplicate 1


title: sample 7: WDR77 siRNA, LNCaP cells, vehicle treated 3

description: sample 7


title: WCB001_WNVE218A_2d_microRNA_4

source_name: Cerebellum, WNVWE218A-inoculated, 2d, bioreplicate 4


title: sample 13: control siRNA, LNCaP cells, R1881 treated 1

description: sample 13


title: WCB001_WNVE218A_4d_microRNA_2

source_name: Cerebellum, WNVWE218A-inoculated, 4d, bioreplicate 2


title: WCB001_WNVWT_4d_microRNA_2

source_name: Cerebellum, WNVWT-inoculated, 4d, bioreplicate 2


title: sample 11: p53 siRNA, LNCaP cells, R1881 treated 3

description: sample 11

source_name: LNCaP cells, R1881 treated 3


title: WCB001_WNVWT_2d_microRNA_5

source_name: Cerebellum, WNVWT-inoculated, 2d, bioreplicate 5


title: WCB001_WNVWT_2d_microRNA_4

source_name: Cerebellum, WNVWT-inoculated, 2d, bioreplicate 4


title: sample 2: p53 siRNA, LNCaP cells, R1881 treated 1

description: sample 2


title: WCB001_Mock_4d_microRNA_5

source_name: Cerebellum, mock-inoculated, 4d, bioreplicate 5

value: Mock


title: WCB001_WNVWT_2d_microRNA_2

source_name: Cerebellum, WNVWT-inoculated, 2d, bioreplicate 2


title: sample 14: WDR77 siRNA, LNCaP cells, R1881 treated 1

description: sample 14

source_name: LNCaP cells, R1881 treated 1


title: sample 19: WDR77 siRNA, LNCaP cells, R1881 treated 3

description: sample 19


title: WCB001_Mock_4d_microRNA_4

source_name: Cerebellum, mock-inoculated, 4d, bioreplicate 4


title: WCB001_WNVWT_4d_microRNA_1

source_name: Cerebellum, WNVWT-inoculated, 4d, bioreplicate 1


title: WCB001_WNVWT_2d_microRNA_3

source_name: Cerebellum, WNVWT-inoculated, 2d, bioreplicate 3


title: sample 1: control siRNA, LNCaP cells, vehicle treated 1

description: sample 1

source_name: LNCaP cells, vehicle treated 1


title: WCB001_Mock_4d_microRNA_2

source_name: Cerebellum, mock-inoculated, 4d, bioreplicate 2


title: WCB001_Mock_4d_microRNA_1

source_name: Cerebellum, mock-inoculated, 4d, bioreplicate 1


title: WCB001_WNVWT_2d_microRNA_1

source_name: Cerebellum, WNVWT-inoculated, 2d, bioreplicate 1


title: WCB001_Mock_2d_microRNA_4

source_name: Cerebellum, mock-inoculated, 2d, bioreplicate 4


title: WCB001_Mock_2d_microRNA_1

source_name: Cerebellum, mock-inoculated, 2d, bioreplicate 1


title: WCB001_Mock_2d_microRNA_2

source_name: Cerebellum, mock-inoculated, 2d, bioreplicate 2


title: WCB001_Mock_2d_microRNA_3

source_name: Cerebellum, mock-inoculated, 2d, bioreplicate 3


title: ICL103_NS1_12h_microRNA_2

status: Public on Feb 20 2016

source_name: Calu-3, NS1-inoculated, 12hr, bioreplicate 2

treatment_protocol: Cells were washed twice with PBS and infected with a multiplicity of infection of 1


title: WCB001_Mock_4d_microRNA_3

source_name: Cerebellum, mock-inoculated, 4d, bioreplicate 3


title: ICL103_NS1_12h_microRNA_3

source_name: Calu-3, NS1-inoculated, 12hr, bioreplicate 3


title: ICL103_NS1_12h_microRNA_4

source_name: Calu-3, NS1-inoculated, 12hr, bioreplicate 4


title: WCB001_Mock_2d_microRNA_5

source_name: Cerebellum, mock-inoculated, 2d, bioreplicate 5


title: ICL103_VN1203_12h_microRNA_3

source_name: Calu-3, VN1203-inoculated, 12hr, bioreplicate 3


title: ICL103_NS1_12h_microRNA_1

source_name: Calu-3, NS1-inoculated, 12hr, bioreplicate 1


title: ICL103_VN1203_12h_microRNA_5

source_name: Calu-3, VN1203-inoculated, 12hr, bioreplicate 5


title: WCB001_WNVWT_4d_microRNA_5

source_name: Cerebellum, WNVWT-inoculated, 4d, bioreplicate 5


title: ICL103_VN1203_12h_microRNA_4

source_name: Calu-3, VN1203-inoculated, 12hr, bioreplicate 4


title: ICL103_Mock_12h_microRNA_4

source_name: Calu-3, mock-inoculated, 12hr, bioreplicate 4


title: ICL103_Mock_12h_microRNA_5

source_name: Calu-3, mock-inoculated, 12hr, bioreplicate 5


title: ICL103_VN1203_12h_microRNA_2

source_name: Calu-3, VN1203-inoculated, 12hr, bioreplicate 2


title: ICL103_NS1_7h_microRNA_3

source_name: Calu-3, NS1-inoculated, 7hr, bioreplicate 3


title: ICL103_NS1_12h_microRNA_5

source_name: Calu-3, NS1-inoculated, 12hr, bioreplicate 5


title: ICL103_NS1_7h_microRNA_2

source_name: Calu-3, NS1-inoculated, 7hr, bioreplicate 2


title: ICL103_VN1203_7h_microRNA_3

source_name: Calu-3, VN1203-inoculated, 7hr, bioreplicate 3


title: ICL103_NS1_7h_microRNA_1

source_name: Calu-3, NS1-inoculated, 7hr, bioreplicate 1


title: ICL103_VN1203_7h_microRNA_5

source_name: Calu-3, VN1203-inoculated, 7hr, bioreplicate 5


title: ICL103_VN1203_7h_microRNA_4

source_name: Calu-3, VN1203-inoculated, 7hr, bioreplicate 4


title: ICL103_Mock_12h_microRNA_1

source_name: Calu-3, mock-inoculated, 12hr, bioreplicate 1


title: ICL103_VN1203_7h_microRNA_2

source_name: Calu-3, VN1203-inoculated, 7hr, bioreplicate 2


title: ICL103_Mock_7h_microRNA_4

source_name: Calu-3, mock-inoculated, 7hr, bioreplicate 4


title: ICL103_Mock_7h_microRNA_2

source_name: Calu-3, mock-inoculated, 7hr, bioreplicate 2


title: ICL103_NS1_7h_microRNA_4

source_name: Calu-3, NS1-inoculated, 7hr, bioreplicate 4


title: ICL103_Mock_12h_microRNA_2

source_name: Calu-3, mock-inoculated, 12hr, bioreplicate 2


title: ICL103_NS1_7h_microRNA_5

source_name: Calu-3, NS1-inoculated, 7hr, bioreplicate 5


title: WCB001_WNVE218A_6d_RNA_2

status: Public on Feb 15 2016

source_name: Cerebellum, WNVE218A-inoculated, 6d, bioreplicate 2


title: ICL103_Mock_12h_microRNA_3

source_name: Calu-3, mock-inoculated, 12hr, bioreplicate 3


title: WCB001_WNVE218A_6d_RNA_4

source_name: Cerebellum, WNVE218A-inoculated, 6d, bioreplicate 4


title: WCB001_WNVE218A_4d_RNA_1

source_name: Cerebellum, WNVE218A-inoculated, 4d, bioreplicate 1


title: WCB001_WNVE218A_2d_RNA_3

source_name: Cerebellum, WNVE218A-inoculated, 2d, bioreplicate 3


title: WCB001_WNVE218A_4d_RNA_4

source_name: Cerebellum, WNVE218A-inoculated, 4d, bioreplicate 4


title: WCB001_WNVE218A_6d_RNA_3

source_name: Cerebellum, WNVE218A-inoculated, 6d, bioreplicate 3


title: ICL103_Mock_7h_microRNA_3

source_name: Calu-3, mock-inoculated, 7hr, bioreplicate 3


title: ICL103_VN1203_12h_microRNA_1

source_name: Calu-3, VN1203-inoculated, 12hr, bioreplicate 1


title: WCB001_WNVE218A_4d_RNA_3

source_name: Cerebellum, WNVE218A-inoculated, 4d, bioreplicate 3


title: WCB001_WNVE218A_6d_RNA_1

source_name: Cerebellum, WNVE218A-inoculated, 6d, bioreplicate 1


title: WCB001_WNVE218A_1d_RNA_5

source_name: Cerebellum, WNVE218A-inoculated, 1d, bioreplicate 5


title: WCB001_WNVE218A_4d_RNA_5

source_name: Cerebellum, WNVE218A-inoculated, 4d, bioreplicate 5


title: WCB001_WNVE218A_6d_RNA_5

source_name: Cerebellum, WNVE218A-inoculated, 6d, bioreplicate 5


title: WCB001_WNVE218A_4d_RNA_2

source_name: Cerebellum, WNVE218A-inoculated, 4d, bioreplicate 2


title: WCB001_WNVE218A_2d_RNA_2

source_name: Cerebellum, WNVE218A-inoculated, 2d, bioreplicate 2


title: WCB001_WNVE218A_2d_RNA_4

source_name: Cerebellum, WNVE218A-inoculated, 2d, bioreplicate 4


title: WCB001_WNVE218A_1d_RNA_4

source_name: Cerebellum, WNVE218A-inoculated, 1d, bioreplicate 4


title: WCB001_WNVE218A_1d_RNA_2

source_name: Cerebellum, WNVE218A-inoculated, 1d, bioreplicate 2


title: WCB001_WNVWT_6d_RNA_4

source_name: Cerebellum, WNVWT-inoculated, 6d, bioreplicate 4


title: WCB001_WNVWT_6d_RNA_1

source_name: Cerebellum, WNVWT-inoculated, 6d, bioreplicate 1


title: WCB001_WNVE218A_2d_RNA_5

source_name: Cerebellum, WNVE218A-inoculated, 2d, bioreplicate 5


title: WCB001_WNVE218A_1d_RNA_1

source_name: Cerebellum, WNVE218A-inoculated, 1d, bioreplicate 1


title: WCB001_WNVWT_6d_RNA_3

source_name: Cerebellum, WNVWT-inoculated, 6d, bioreplicate 3


title: WCB001_WNVE218A_1d_RNA_3

source_name: Cerebellum, WNVE218A-inoculated, 1d, bioreplicate 3


title: WCB001_WNVE218A_2d_RNA_1

source_name: Cerebellum, WNVE218A-inoculated, 2d, bioreplicate 1


title: WCB001_WNVWT_6d_RNA_5

source_name: Cerebellum, WNVWT-inoculated, 6d, bioreplicate 5


title: WCB001_WNVWT_6d_RNA_2

source_name: Cerebellum, WNVWT-inoculated, 6d, bioreplicate 2


title: WCB001_WNVWT_4d_RNA_4

source_name: Cerebellum, WNVWT-inoculated, 4d, bioreplicate 4


title: WCB001_WNVWT_4d_RNA_1

source_name: Cerebellum, WNVWT-inoculated, 4d, bioreplicate 1


title: WCB001_WNVWT_1d_RNA_4

source_name: Cerebellum, WNVWT-inoculated, 1d, bioreplicate 4


title: WCB001_WNVWT_4d_RNA_2

source_name: Cerebellum, WNVWT-inoculated, 4d, bioreplicate 2


title: WCB001_WNVWT_2d_RNA_4

source_name: Cerebellum, WNVWT-inoculated, 2d, bioreplicate 4


title: WCB001_WNVWT_4d_RNA_5

source_name: Cerebellum, WNVWT-inoculated, 4d, bioreplicate 5


title: WCB001_WNVWT_2d_RNA_1

source_name: Cerebellum, WNVWT-inoculated, 2d, bioreplicate 1


title: WCB001_Mock_6d_RNA_5

source_name: Cerebellum, mock-inoculated, 6d, bioreplicate 5


title: WCB001_WNVWT_4d_RNA_3

source_name: Cerebellum, WNVWT-inoculated, 4d, bioreplicate 3


title: WCB001_WNVWT_1d_RNA_5

source_name: Cerebellum, WNVWT-inoculated, 1d, bioreplicate 5


title: WCB001_WNVWT_1d_RNA_3

source_name: Cerebellum, WNVWT-inoculated, 1d, bioreplicate 3


title: WCB001_WNVWT_1d_RNA_2

source_name: Cerebellum, WNVWT-inoculated, 1d, bioreplicate 2


title: WCB001_WNVWT_2d_RNA_3

source_name: Cerebellum, WNVWT-inoculated, 2d, bioreplicate 3


title: WCB001_WNVWT_2d_RNA_5

source_name: Cerebellum, WNVWT-inoculated, 2d, bioreplicate 5


title: WCB001_WNVWT_1d_RNA_1

source_name: Cerebellum, WNVWT-inoculated, 1d, bioreplicate 1


title: WCB001_Mock_6d_RNA_1

source_name: Cerebellum, mock-inoculated, 6d, bioreplicate 1


title: WCB001_Mock_6d_RNA_3

source_name: Cerebellum, mock-inoculated, 6d, bioreplicate 3


title: WCB001_Mock_4d_RNA_1

source_name: Cerebellum, mock-inoculated, 4d, bioreplicate 1


title: WCB001_Mock_4d_RNA_4

source_name: Cerebellum, mock-inoculated, 4d, bioreplicate 4


title: WCB001_Mock_4d_RNA_3

source_name: Cerebellum, mock-inoculated, 4d, bioreplicate 3


title: WCB001_Mock_6d_RNA_4

source_name: Cerebellum, mock-inoculated, 6d, bioreplicate 4


title: WCB001_WNVWT_2d_RNA_2

source_name: Cerebellum, WNVWT-inoculated, 2d, bioreplicate 2


title: WCB001_Mock_4d_RNA_5

source_name: Cerebellum, mock-inoculated, 4d, bioreplicate 5


title: WCB001_Mock_2d_RNA_5

source_name: Cerebellum, mock-inoculated, 2d, bioreplicate 5


title: WCB001_Mock_2d_RNA_2

source_name: Cerebellum, mock-inoculated, 2d, bioreplicate 2


title: WCB001_Mock_6d_RNA_2

source_name: Cerebellum, mock-inoculated, 6d, bioreplicate 2


title: WCB001_Mock_2d_RNA_1

source_name: Cerebellum, mock-inoculated, 2d, bioreplicate 1


title: skin  antigen (animal n°1 sample7)

status: Public on Jan 31 2016

city: Berlin

first: Hans-Joachim

last: Mollenkopf

address: Charitéplatz 1

country: Germany

institute: Max-Planck-Institute for Infection Biology

hyb_protocol: After precipitation, purification and quantification, 1.25µg labeled samples were  hybridized according to the supplier’s protocol (Agilent Technologies).

scan_protocol: Scanning of microarrays was performed with 5µm resolution and extended range using a G2565CA high resolution laser microarray scanner (Agilent Technologies).

data_processing: Microarray image data were extracted and analyzed with the Image Analysis / Feature Extraction software G2567AA (Version A.10.10.1, Agilent Technologies). The extracted MAGE-ML files were further analyzed with the Rosetta Resolver, Build 7.2.2.0 SP1.31 (Rosetta Biosoftware, Seattle, USA).

organism: Sus scrofa

source_name: skin

label_protocol: Total RNA labeling was performed with the single color Quick Amp Labeling Kit (Agilent Technologies).

extract_protocol: Animals were anesthetized at 4h post-immunization using Ketamin/Dormicum (4 animals per formulations/controls). Punch biopsies of skin were sampled using 8 mm diameter biopsy punch and frozen in liquid nitrogen. Total RNA from skin was extracted using TRI Reagent® (Ambion) method according to supplier's instructions after homogenization using Ultra Turrax homogenizer. Finally, total RNA was extracted again by column-based method using Nucleospin II RNA kit (Macherey-Nagel).

treatment_protocol: Pigs (Sus scrofa domestica, line TOPIG 20, 9 week old male animals, Lelystad) were first tested free of influenza antibodies using influenza hemagglutination inhibition assay. Animals were then anesthetized with Propofol and were immunized at various sites of the skin with various formulations (a total of 7 sites per animal). The formulations of vaccines were all done in phosphate buffered saline [PBS].  The TLR5 agonist: the flagellin FliC from Salmonella enterica serovar Typhimurium was administered at various doses  (0.1 to 10 micrograms) in combination with 15 micrograms of HA antigen from H1N1 influenza virus (cGMP grade) diluted in 100 microliters of PBS. Control sites were also injected with PBS alone or HA antigen from H1N1 in PBS. The intradermal administration was done using 29G x 1/2 hypodermic needle. The skin area of injection was marked for later sampling the site of flagellin administration. Finally, mock skin, i.e. un-injected skin was used as an additional negative control for microarrays.

value: skin

value: 15µg H1 N1 + PBS buffer

tag: line

value: TOPIG 20

tag: Sex

value: male

value: 9 weeks-old


title: WCB001_Mock_1d_RNA_3

source_name: Cerebellum, mock-inoculated, 1d, bioreplicate 3

value: 5 weeks

tag: time


title: WCB001_Mock_1d_RNA_1

source_name: Cerebellum, mock-inoculated, 1d, bioreplicate 1

tag: virus


title: skin antigen (animal n°4 sample7)


title: skin  mock (animal n°2 sample9)

value: PBS buffer


title: skin  antigen (animal n°3 sample7)


title: WCB001_Mock_1d_RNA_2

source_name: Cerebellum, mock-inoculated, 1d, bioreplicate 2


title: WCB001_Mock_1d_RNA_5

source_name: Cerebellum, mock-inoculated, 1d, bioreplicate 5


title: skin  mock (animal n°3 sample9)


title: skin  FliC 10 (animal n°4 sample3)

value: 10µg flagellin + 15µg H1N1 + PBS buffer


title: skin  FliC 10 (animal n°1 sample3)


title: skin  mock (animal n°3 sample8)


title: skin  mock (animal n°4 sample9)


title: skin  FliC 10 (animal n°3 sample3)


title: WCB001_Mock_1d_RNA_4

source_name: Cerebellum, mock-inoculated, 1d, bioreplicate 4


title: skin  FliC 10 (animal n°2 sample3)


title: skin  FliC 3 (animal n°3 sample4)

value: 3µg flagellin + 15µg H1N1 + PBS buffer


title: WCB001_Mock_2d_RNA_4

source_name: Cerebellum, mock-inoculated, 2d, bioreplicate 4


title: skin  mock (animal n°2 sample8)


title: skin  FliC 3 (animal n°1 sample4)


title: skin  mock (animal n°1 sample9)


title: skin  antigen (animal n°2 sample7)


title: skin  mock (animal n°4 sample8)


title: skin  FliC 3 (animal n°2 sample4)


title: skin  mock (animal n°1 sample8)


title: WCB001_Mock_2d_RNA_3

source_name: Cerebellum, mock-inoculated, 2d, bioreplicate 3


title: skin  FliC 1 (animal n°2 sample5)

value: 1µg flagellin + 15µg H1N1 + PBS buffer


title: skin  FliC 1 (animal n°3 sample5)


title: skin  FliC 1 (animal n°1 sample5)


title: skin  FliC 0.3 (animal n°1 sample6)

value: 0.3µg flagellin + 15µg H1N1 + PBS buffer


title: skin  FliC 0.3 (animal n°4 sample6)


title: ICL103_NS1_24hr_4

status: Public on Jan 30 2016

source_name: Calu-3, NS1-inoculated, 24hr, bioreplicate 4


title: skin  FliC 0.1 (animal n°3 sample2)

value: 0.1µg flagellin + 15µg H1N1 + PBS buffer


title: skin  FliC 0.3 (animal n°3 sample6)


title: skin  FliC 0.3 (animal n°2 sample6)


title: skin  FliC 0.1 (animal n°1 sample2)


title: skin  FliC 0.1 (animal n°4 sample2)


title: ICL103_NS1_24hr_3

source_name: Calu-3, NS1-inoculated, 24hr, bioreplicate 3


title: skin  FliC 1 (animal n°4 sample5)


title: ICL103_NS1_24hr_1

source_name: Calu-3, NS1-inoculated, 24hr, bioreplicate 1


title: ICL103_NS1_24hr_2

source_name: Calu-3, NS1-inoculated, 24hr, bioreplicate 2


title: ICL103_627E_24hr_4

source_name: Calu-3, 627E-inoculated, 24hr, bioreplicate 4


title: ICL103_VN1203_24hr_5

source_name: Calu-3, VN1203-inoculated, 24hr, bioreplicate 5


title: ICL103_627E_24hr_1

source_name: Calu-3, 627E-inoculated, 24hr, bioreplicate 1


title: skin  FliC 0.1 (animal n°2 sample2)


title: ICL103_627E_24hr_3

source_name: Calu-3, 627E-inoculated, 24hr, bioreplicate 3


title: skin  FliC 3 (animal n°4 sample4)


title: ICL103_VN1203_24hr_4

source_name: Calu-3, VN1203-inoculated, 24hr, bioreplicate 4


title: ICL103_VN1203_24hr_3

source_name: Calu-3, VN1203-inoculated, 24hr, bioreplicate 3


title: ICL103_627E_24hr_5

source_name: Calu-3, 627E-inoculated, 24hr, bioreplicate 5


title: ICL103_Mock_24hr_4

source_name: Calu-3, mock-inoculated, 24hr, bioreplicate 4


title: ICL103_Mock_24hr_3

source_name: Calu-3, mock-inoculated, 24hr, bioreplicate 3


title: WCB001_Mock_4d_RNA_2

source_name: Cerebellum, mock-inoculated, 4d, bioreplicate 2


title: ICL103_NS1_12hr_2

source_name: Calu-3, NS1-inoculated, 12hr, bioreplicate 2


title: ICL103_VN1203_24hr_1

source_name: Calu-3, VN1203-inoculated, 24hr, bioreplicate 1


title: ICL103_VN1203_12hr_5

source_name: Calu-3, VN1203-inoculated, 12hr, bioreplicate 5


title: ICL103_627E_24hr_2

source_name: Calu-3, 627E-inoculated, 24hr, bioreplicate 2


title: ICL103_Mock_24hr_5

source_name: Calu-3, mock-inoculated, 24hr, bioreplicate 5


title: ICL103_NS1_12hr_3

source_name: Calu-3, NS1-inoculated, 12hr, bioreplicate 3


title: ICL103_627E_12hr_4

source_name: Calu-3, 627E-inoculated, 12hr, bioreplicate 4


title: ICL103_Mock_24hr_1

source_name: Calu-3, mock-inoculated, 24hr, bioreplicate 1


title: ICL103_NS1_12hr_4

source_name: Calu-3, NS1-inoculated, 12hr, bioreplicate 4


title: ICL103_NS1_12hr_1

source_name: Calu-3, NS1-inoculated, 12hr, bioreplicate 1


title: ICL103_VN1203_12hr_2

source_name: Calu-3, VN1203-inoculated, 12hr, bioreplicate 2


title: ICL103_NS1_24hr_5

source_name: Calu-3, NS1-inoculated, 24hr, bioreplicate 5


title: ICL103_VN1203_12hr_4

source_name: Calu-3, VN1203-inoculated, 12hr, bioreplicate 4


title: ICL103_Mock_24hr_2

source_name: Calu-3, mock-inoculated, 24hr, bioreplicate 2


title: ICL103_627E_12hr_2

source_name: Calu-3, 627E-inoculated, 12hr, bioreplicate 2


title: ICL103_VN1203_12hr_3

source_name: Calu-3, VN1203-inoculated, 12hr, bioreplicate 3


title: ICL103_627E_12hr_5

source_name: Calu-3, 627E-inoculated, 12hr, bioreplicate 5


title: ICL103_NS1_12hr_5

source_name: Calu-3, NS1-inoculated, 12hr, bioreplicate 5


title: ICL103_627E_12hr_1

source_name: Calu-3, 627E-inoculated, 12hr, bioreplicate 1


title: ICL103_627E_12hr_3

source_name: Calu-3, 627E-inoculated, 12hr, bioreplicate 3


title: ICL103_VN1203_12hr_1

source_name: Calu-3, VN1203-inoculated, 12hr, bioreplicate 1


title: ICL103_Mock_12hr_5

source_name: Calu-3, mock-inoculated, 12hr, bioreplicate 5


title: ICL103_Mock_12hr_4

source_name: Calu-3, mock-inoculated, 12hr, bioreplicate 4


title: ICL103_Mock_12hr_2

source_name: Calu-3, mock-inoculated, 12hr, bioreplicate 2


title: ICL103_Mock_12hr_3

source_name: Calu-3, mock-inoculated, 12hr, bioreplicate 3


title: ICL103_NS1_7hr_3

source_name: Calu-3, NS1-inoculated, 7hr, bioreplicate 3


title: ICL103_NS1_7hr_1

source_name: Calu-3, NS1-inoculated, 7hr, bioreplicate 1


title: ICL103_Mock_7hr_5

source_name: Calu-3, mock-inoculated, 7hr, bioreplicate 5


title: ICL103_627E_7hr_1

source_name: Calu-3, 627E-inoculated, 7hr, bioreplicate 1


title: ICL103_NS1_7hr_5

source_name: Calu-3, NS1-inoculated, 7hr, bioreplicate 5


title: ICL103_NS1_7hr_4

source_name: Calu-3, NS1-inoculated, 7hr, bioreplicate 4


title: ICL103_627E_7hr_2

source_name: Calu-3, 627E-inoculated, 7hr, bioreplicate 2


title: ICL103_627E_7hr_3

source_name: Calu-3, 627E-inoculated, 7hr, bioreplicate 3


title: ICL103_VN1203_7hr_1

source_name: Calu-3, VN1203-inoculated, 7hr, bioreplicate 1


title: ICL103_VN1203_7hr_4

source_name: Calu-3, VN1203-inoculated, 7hr, bioreplicate 4


title: ICL103_NS1_7hr_2

source_name: Calu-3, NS1-inoculated, 7hr, bioreplicate 2


title: ICL103_Mock_12hr_1

source_name: Calu-3, mock-inoculated, 12hr, bioreplicate 1


title: ICL103_Mock_7hr_1

source_name: Calu-3, mock-inoculated, 7hr, bioreplicate 1


title: ICL103_Mock_7hr_2

source_name: Calu-3, mock-inoculated, 7hr, bioreplicate 2


title: ICL103_NS1_0hr_4

source_name: Calu-3, NS1-inoculated, 0hr, bioreplicate 4


title: ICL103_VN1203_7hr_3

source_name: Calu-3, VN1203-inoculated, 7hr, bioreplicate 3


title: ICL103_627E_7hr_4

source_name: Calu-3, 627E-inoculated, 7hr, bioreplicate 4


title: ICL103_Mock_7hr_4

source_name: Calu-3, mock-inoculated, 7hr, bioreplicate 4


title: ICL103_Mock_7hr_3

source_name: Calu-3, mock-inoculated, 7hr, bioreplicate 3


title: ICL103_NS1_0hr_5

source_name: Calu-3, NS1-inoculated, 0hr, bioreplicate 5


title: ICL103_VN1203_7hr_2

source_name: Calu-3, VN1203-inoculated, 7hr, bioreplicate 2


title: ICL103_NS1_0hr_2

source_name: Calu-3, NS1-inoculated, 0hr, bioreplicate 2


title: ICL103_627E_0hr_5

source_name: Calu-3, 627E-inoculated, 0hr, bioreplicate 5


title: ICL103_627E_0hr_3

source_name: Calu-3, 627E-inoculated, 0hr, bioreplicate 3


title: ICL103_NS1_0hr_3

source_name: Calu-3, NS1-inoculated, 0hr, bioreplicate 3


title: ICL103_NS1_0hr_1

source_name: Calu-3, NS1-inoculated, 0hr, bioreplicate 1


title: ICL103_627E_0hr_4

source_name: Calu-3, 627E-inoculated, 0hr, bioreplicate 4


title: ICL103_VN1203_0hr_5

source_name: Calu-3, VN1203-inoculated, 0hr, bioreplicate 5


title: ICL103_VN1203_0hr_4

source_name: Calu-3, VN1203-inoculated, 0hr, bioreplicate 4


title: ICL103_Mock_0hr_3

source_name: Calu-3, mock-inoculated, 0hr, bioreplicate 3


title: ICL103_VN1203_0hr_2

source_name: Calu-3, VN1203-inoculated, 0hr, bioreplicate 2


title: ICL103_Mock_0hr_5

source_name: Calu-3, mock-inoculated, 0hr, bioreplicate 5


title: WDC011_WNVE218A_MOI500_24h_2

status: Public on Dec 31 2015

growth_protocol: WNV009.0P: Preparing myeloid dendritic cells

extract_protocol: WNV003.0P: Harvesting protocol for panomics of mDC

treatment_protocol: Cells were infected with a multiplicity of infection of 500.

value: primary myeloid dendritic cells

tag: time (h post-infection)


title: ICL103_Mock_0hr_2

source_name: Calu-3, mock-inoculated, 0hr, bioreplicate 2


title: ICL103_VN1203_24hr_2

source_name: Calu-3, VN1203-inoculated, 24hr, bioreplicate 2


title: ICL103_627E_0hr_2

source_name: Calu-3, 627E-inoculated, 0hr, bioreplicate 2


title: ICL103_VN1203_0hr_3

source_name: Calu-3, VN1203-inoculated, 0hr, bioreplicate 3


title: WDC011_WNVE218A_MOI500_24h_3

tag: cell type


title: ICL103_VN1203_7hr_5

source_name: Calu-3, VN1203-inoculated, 7hr, bioreplicate 5


title: WDC011_WNVE218A_MOI500_12h_2


title: ICL103_Mock_0hr_1

source_name: Calu-3, mock-inoculated, 0hr, bioreplicate 1


title: WDC011_WNVE218A_MOI500_24h_1


title: WDC011_WNVE218A_MOI500_12h_1


title: ICL103_VN1203_0hr_1

source_name: Calu-3, VN1203-inoculated, 0hr, bioreplicate 1


title: WDC011_WNVE218A_MOI500_12h_3


title: ICL103_627E_0hr_1

source_name: Calu-3, 627E-inoculated, 0hr, bioreplicate 1


title: WDC011_WNVE218A_MOI500_8h_3


title: WDC011_WNVE218A_MOI500_8h_2


title: WDC011_WNVE218A_MOI500_8h_1


title: WDC011_WNVE218A_MOI500_1h_1


title: ICL103_627E_7hr_5

source_name: Calu-3, 627E-inoculated, 7hr, bioreplicate 5


title: WDC011_WNVMT_MOI500_24h_2

value: WNVMT


title: WDC011_mockulum_MOI500_12h_1

value: Mockulum


title: WDC011_WNVMT_MOI500_24h_3


title: WDC011_WNVMT_MOI500_1h_2


title: WDC011_WNVE218A_MOI500_1h_2


title: WDC011_WNVE218A_MOI500_1h_3


title: WDC011_mockulum_MOI500_8h_3


title: ICL103_Mock_0hr_4

source_name: Calu-3, mock-inoculated, 0hr, bioreplicate 4


title: WDC011_WNVMT_MOI500_12h_3


title: WDC011_WNVMT_MOI500_8h_3


title: WDC011_WNVMT_MOI500_8h_1


title: WDC011_WNVMT_MOI500_12h_1


title: WDC011_mockulum_MOI500_1h_2


title: WDC011_WNVMT_MOI500_8h_2


title: WDC011_mockulum_MOI500_1h_3


title: WDC010_WNVE218A_MOI500_12h_3_RNA

status: Public on Nov 04 2015

value: primary myeloid dendritic cell


title: WDC010_WNVE218A_MOI500_24h_2_RNA


title: WDC010_WNVE218A_MOI500_12h_2_RNA


title: WDC011_mockulum_MOI500_24h_2


title: WDC011_WNVMT_MOI500_1h_3


title: WDC011_WNVMT_MOI500_12h_2


title: WDC011_mockulum_MOI500_24h_3


title: WDC011_mockulum_MOI500_24h_1


title: WDC011_mockulum_MOI500_8h_2


title: WDC010_WNVE218A_MOI500_24h_3_RNA


title: WDC011_mockulum_MOI500_1h_1


title: WDC010_WNVE218A_MOI500_8h_2_RNA


title: WDC010_WNVE218A_MOI500_1h_1_RNA


title: WDC010_WNVMT_MOI500_24h_1_RNA


title: WDC010_WNVMT_MOI500_12h_1_RNA


title: WDC010_WNVMT_MOI500_12h_3_RNA


title: WDC010_WNVMT_MOI500_8h_2_RNA


title: WDC011_mockulum_MOI500_8h_1


title: WDC011_WNVMT_MOI500_1h_1