title: H2195(A)

status: Public on Mar 07 2007

city: Dallas

first: Luc

last: Girard

state: TX

address: 5323 Harry Hines Blvd

department: Hamon Center for Therapeutic Oncology Research

institute: UT Southwestern Medical Center

description: lung cancer cell line

hyb_protocol: Following fragmentation, 20 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station

scan_protocol: GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A

data_processing: Data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250

source_name: lung cancer cell line

label_protocol: Biotinylated cRNA was prepared according to standard Affymetrix protocol from 5 microgram total RNA

growth_protocol: RPMI 5% BFS

extract_protocol: Qiagen RNeasy kit including DNase1 treatment

tag: Histology

value: SCLC

tag: Age

tag: Sex

value: M

tag: Race

value: Caucasian


title: SAEC(A)

type: RNA

country: USA

description: Unimmortalized control

label: biotin

molecule: total RNA

organism: Homo sapiens

source_name: Small Airway Epithelial Cell

growth_protocol: SAGM media from Clonetics

value: immortalized normal

tag: Age:

value: F

tag: Race:


title: HCC1833(A)

description: lung cancer cell line

value: adenocarcinoma


title: H82(A)

description: lung cancer cell line

value: caucasian


title: NHBEC(A)

description: Unimmortalized control

source_name: Normal Human Bronchial Epithelial Cell

growth_protocol: BEGM media from Clonetics


title: HCC970(A)

description: lung cancer cell line


title: HCC2429(A)

description: lung cancer cell line

value: NSCLC


title: HCC33(A)

description: lung cancer cell line


title: H661(A)

description: lung cancer cell line

value: large cell carcinoma


title: H23(A)

description: lung cancer cell line

value: black


title: H3122(A)

description: lung cancer cell line

tag: Sex:


title: H841f(A)

description: lung cancer cell line


title: H289(A)

description: lung cancer cell line


title: H28(A)

description: lung cancer cell line

value: mesothelioma


title: H524(A)

description: lung cancer cell line


title: H2227(A)

description: lung cancer cell line


title: H322(A)

description: lung cancer cell line

value: bronchoalveolar


title: HCC2450(A)

description: lung cancer cell line

value: squamous cell carcinoma


title: H2171(A)

description: lung cancer cell line


title: H2107(A)

description: lung cancer cell line


title: H2052(A)

description: lung cancer cell line


title: H2141(A)

description: lung cancer cell line


title: H526(A2)

description: lung cancer cell line


title: H378(A)

description: lung cancer cell line


title: H1963(A)

description: lung cancer cell line


title: H187(A)

description: lung cancer cell line


title: H209(A2)

description: lung cancer cell line


title: H1792(A)

description: lung cancer cell line


title: Human Uninfected Sample N141-CD4+ T cells

status: Public on Feb 23 2007

city: Toronto

first: Martin

last: Hyrcza

state: Ontario

address: 1 King's College Circle, Rm 6356

country: Canada

institute: University of Toronto

hyb_protocol: 20 µg of biotin-labeled cRNA was fragmented following GeneChip Sample Cleanup Module protocol (cat. # 900371, Affymetrix). Fragmented cRNA was hybridized to Hu133A microarrays (Affymetrix) for 16hr in GeneChip Hybridization Oven 640 (Affymetrix) in hybridization cocktail. Washing, staining and scanning were performed on-site using Fluidics Station 400 (Affymetrix) for washes.

scan_protocol: GeneArray Scanner (Agilent) was used for scans. Initial image processing was performed using GCOS ver. 1.2 (Affymetrix) with the following settings Alpha1: 0.05, Alpha2: 0.065, Tau: 0.015, Gamma1H: 0.0045, Gamma1L: 0.0045, Gamma2H: 0.006, Gamma2L: 0.006, Target: 500, NF: 1.

source_name: CD4+ T cells derived by negative selection from leukopheresed blood sample

label_protocol: 5-6 µg of total RNA was reverse-transcribed using SuperScript II reverse transcriptase (Invitrogen) and second strand synthesis was performed using SuperScript Double-Stranded cDNA Synthesis Kit (cat. # 11917-010, Invitrogen) per manufacturer’s instructions. cDNA was purified using cDNA cleanup columns of the GeneChip Sample Cleanup Module (cat. # 900371, Affymetrix). In vitro transcription of biotin-labeled cRNA was performed with the BioArray HighYield RNA Transcript Labeling kit (cat. # 900182, Enzo Life Sciences) following manufacturer’s instructions. cRNA was purified using IVT cleanup columns of the GeneChip Sample Cleanup Module (cat. # 900371, Affymetrix)

extract_protocol: Qiagen RNeasy kit

treatment_protocol: Morning leukopheresis, 100ml, followed by standard Ficoll protocol, buffy coat separation and isolation of CD4+ T cells using negative selection kit from StemCell (StemSep Human CD4+ T Cell Enrichment Cocktail).

tag: Male, 32 years old


title: H1155f(A)

description: lung cancer cell line


title: H128(A)

description: lung cancer cell line


title: Human Uninfected Sample N050-CD4+ T cells

tag: Male, 27 years old


title: Human Uninfected Sample N001-CD8+ T cells

source_name: CD8+ T cells derived by negative selection from leukopheresed blood sample

treatment_protocol: Morning leukopheresis, 100ml, followed by standard Ficoll protocol, buffy coat separation and isolation of CD8+ T cells using negative selection kit from StemCell (StemSep Human CD8+ T Cell Enrichment Cocktail).

tag: Male, 42 years old


title: Human Uninfected Sample N001-CD4+ T cells


title: H1184(A)

description: lung cancer cell line


title: BEAS-2B(A)

description: Immortalized with SV40 Large T antigen

source_name: Human Bronchial Epithelial Cell


title: H146(A2)

description: lung cancer cell line


title: Human Non-progressor HIV Sample L128-CD4+ T cells

tag: Male, 29 years old, HIV infection of 3.5 years


title: Human Uninfected Sample N141-CD8+ T cells


title: Human Non-progressor HIV Sample L134-CD8+ T cells

tag: Male, 44 years old, HIV infection of 12 years


title: Human Uninfected Sample N110-CD8+ T cells

tag: Male, 43 years old


title: Human Non-progressor HIV Sample L128-CD8+ T cells


title: Human Uninfected Sample N110-CD4+ T cells


title: Human Uninfected Sample N071-CD4+ T cells

tag: Male, 34 years old


title: Human Non-progressor HIV Sample L134-CD4+ T cells


title: Human Non-progressor HIV Sample L015-CD8+ T cells

tag: Male, 39 years old, HIV infection of 11 years


title: Human Non-progressor HIV Sample L015-CD4+ T cells


title: Human Non-progressor HIV Sample L115-CD8+ T cells

tag: Male, 34 years old, HIV infection of 9 years


title: H1607(A)

description: lung cancer cell line


title: Human Uninfected Sample N071-CD8+ T cells


title: Human Uninfected Sample N050-CD8+ T cells


title: SNB-75 hg133a21

status: Public on Mar 26 2007

city: Bethesda

first: Uma

middle: T

last: Shankavaram

state: MD

address: 9000 Rockville Pike

department: NCI

institute: NIH

description:
Background Avg = 50.69
Background Max = 53.3
Background Min = 49.1
Background Stdev = 0.94
Central- Avg = 1131
Central- Count = 9
Corner+ Avg = 29
Corner+ Count = 32
Corner- Avg = 1179
Corner- Count = 32
Noise Avg = 2.36
Noise Max = 2.9
Noise Min = 2.0
Noise Stdev = 0.13
RawQ = 2.22
Columns = 712
Number of Cells = 506944
Rows = 712
OrganismPart: central nervous system

scan_protocol: Affymetrix CEL analysis (Percentile); Protocol Type = feature_extraction; Parameter CellMargin = 2; Parameter OutlierHigh = 1.500; Parameter OutlierLow = 1.004; Parameter Percentile = 75; Software: Affymetrix MicroArraySuite, type: feature_extraction_software; Protocol Type = image_acquisition; Software: Affymetrix MicroArraySuite, type: image_acquisition_software; Hardware: ;

data_processing: ExpressionStat; Protocol Type = bioassay_data_transformation; Parameter Alpha1 = 0.05; Parameter Alpha2 = 0.065; Parameter BG = 4; Parameter Epsilon = 0.5; Parameter Gamma1H = 0.0045; Parameter Gamma1L = 0.0045; Parameter Gamma2H = 0.006; Parameter Gamma2L = 0.006; Parameter HZ = 4; Parameter NF = 1.000000000000; Parameter Perturbation = 1.1; Parameter SF = 1.000000000000; Parameter SmoothFactorBG = 100; Parameter Tau = 0.015; Parameter VZ = 4; Software: Affymetrix GeneChip Operating Software, type: bioassay_data_transformation_software;

source_name: astrocytoma

label_protocol: Affymetrix Labeling Protocol; The exact protocol is not available. But Standard Operating Proceadure can be found at          http://www.affymetrix.com/support/technical/manual/expression_manual.affx         or         http://www.affymetrix.com/Auth/support/downloads/manuals/expression_ever_manual.zip         Manual number: 701025 Revision 6, page 2.1.3; Protocol Type = labeling;

growth_protocol: NCI60Adherent; RNA harvesting protocol for adherent cells           Media used:         RPMI 1640 500 ml          FBS 25 ml -use the DTP serum if possible, from Bio Whittaker, not heat         inactivated          200 mM Glutamine 5 ml           1 flask = ~15 x 106 cells yields ~ 100 ugr RNA. Grow 10 flasks.                    Cells: start from growing cells from Frederick (not frozen). Started before at         passage #8-12. Do not use past passage 20.                    Growth schedule prior to harvest: Grow cells to         ~80 confluencey. Trypsinize cells with 5 ml try-EDTA per T162, 15 min., 370C. Pipet up and         down several times w 10 ml pipet to get good dispersment of cells. Count cells. Pass cells         to as many flasks as there are cells for. When passing cells, combine flasks into a single         pool. Pass 1x106 cells into each T162 w 30 ml media.  Repeat growth cycle until 10 flasks         are available.   Refeed Refeed cells the day prior to harvest without harvesting).  Draw off         media (wo cells). Add back media, 30 ml per T162. Add back to T-162âs. 37 deg C, ON  Harvest         Target confluency 80%  # of flasks = 10   Draw off media from 1st flask. Lyse cells in 15         ml lysis buffer (w 10 ul fresh BME per ml). Scrape cells. Draw off media from 2nd flask.         Pipet lysis buffer from flask 1 into flask 2. Repeat lysis with up to 4 T162âs. Pipet into         50 ml tube.  Repeat w next set of 4 flasks.  When done, vortex 10 sec.. Draw lysate thru a         20 guage needle 12xâs. Freeze at ö800C.  Purify using Quiagen Midi Kit.  Use a maximun of         100 x 106 cells per column. More will not bind to the column.                           Company Info         ------------           Quiagen Midi Kit Cat # 75144  RLT Buffer (lysis buffer) Cat. # 79216 $60-list, $51- discounted 1-800-362-7737                    Fetal Bovine Serum 500 ml, list $261- Cat #14-502F Lot # 9S083F Cambrex (old Bio Whittaker) 1-800-638-8174                   1x PBS pH 7.3-7.5 Bio Whittaker cat #17-516F $5.30 per 500 ml bottle (1-11)                    250 ml conical tubes Corning cat #25350-250                    RPMI-1640 wo L-glutamine cat # 12-167F  $13.25 per 500 ml bottle (for 1-11 bottles) Cambrex (old Bio Whittaker) Walkersville, Md. 21793 301-898-7025 1-800-638-8174                  Trypsin (0.05%)-EDTA (0.1%) In phosphate buffered saline without calcium and magnesium. cat # 118-087-061 $4.20 per 100 ml bottle Quality Biological, Inc. 301-840-9331                    L-glutamine 200 mM, 100x Gibco-BRL cat # 25030-149 20 ml 1-800-828-6686                    162 cm2 flask cat # 3150                    Costar Tissue Culture Cell Scrapper, 25 cm Sarstedt Cat. # 83.1830                    Cells are received from Nick Scudiero,  E-mail: SCUDIERO@dtpax2.ncifcrf.gov          Also trypsin, FBS and glutamine have been coming from there as well.; Protocol Type = grow;

extract_protocol: NCI60 Extraction Protocol; Harvest         Target confluency  80%            # of flasks = 10                  Draw off media from 1st flask.         Lyse cells in 15 ml lysis buffer (w 10 ul fresh BME per ml).         Scrape cells.         Draw off media from 2nd flask.         Pipet lysis buffer from flask 1 into flask 2.         Repeat lysis with up to 4 T162’s.         Pipet into 50 ml tube.                  Repeat w next set of 4 flasks.                  When done, vortex 10 sec..         Draw lysate thru a 20 guage needle 12x’s.         Freeze at –800C.                  Purify using Quiagen Midi Kit.          Use a maximun of 100 x 106 cells per column. More will not bind to the column.         ; Protocol Type = nucleic_acid_extraction;

tag: age

value: unknown

value: astrocytoma

tag: sex

value: female


title: Human Non-progressor HIV Sample L115-CD4+ T cells


title: Human Chronic HIV Sample C102-CD8+ T cells

tag: Male, 33 years old, HIV infection of >3 years


title: Human Non-progressor HIV Sample L004-CD8+ T cells

tag: Male, 59 years old, HIV infection of 15 years


title: Human Acute HIV Sample A189-CD4+ T cells

tag: Male, 32 years old, HIV infection of ~4 months


title: Human Chronic HIV Sample C101-CD8+ T cells

tag: Male, 32 years old, HIV infection of >2 years


title: Human Chronic HIV Sample C080-CD4+ T cells

tag: Male, 40 years old, HIV infection of 7 years


title: Human Chronic HIV Sample C102-CD4+ T cells


title: Human Acute HIV Sample A144-CD8+ T cells

tag: Male, 27 years old, HIV infection of ~1 months


title: Human Non-progressor HIV Sample L004-CD4+ T cells


title: Human Chronic HIV Sample C138-CD4+ T cells

tag: Male, 39 years old, HIV infection of 2 years


title: Human Chronic HIV Sample C062-CD8+ T cells

tag: Male, 36 years old, HIV infection of >1 year


title: Human Chronic HIV Sample C101-CD4+ T cells


title: Human Acute HIV Sample A051-CD4+ T cells

scan_protocol: GeneArray Scanner (Agilent) was used for scans. Initial image processing was performed using GCOS ver. 1.2 (Affymetrix) with the following settings Alpha1: 0.05, Alpha2: 0.065, Tau: 0.015, Gamma1H: 0.0045, Gamma1L: 0.0045, Gamma2H: 0.006, Gamma2L: 0.006, Target: 500, NF: 1. Scaling factors ranged from 1.2 to 4.8

data_processing: MAS5 algorithm, Alpha1: 0.05, Alpha2: 0.065, Tau: 0.015, Gamma1H: 0.0045, Gamma1L: 0.0045, Gamma2H: 0.006, Gamma2L: 0.006, Target: 500, NF: 1. Scaling factors ranged from 1.2 to 4.8

tag: Male, 49 years old, HIV infection of ~3 months


title: Human Acute HIV Sample A064-CD8+ T cells

tag: Male, 26 years old, HIV infection of ~5 months


title: Human Chronic HIV Sample C080-CD8+ T cells


title: Human Chronic HIV Sample C062-CD4+ T cells


title: Human Acute HIV Sample A064-CD4+ T cells


title: Human Acute HIV Sample A189-CD8+ T cells


title: Human Chronic HIV Sample C138-CD8+ T cells


title: Human Acute HIV Sample A044-CD8+ T cells

tag: Male, 25 years old, HIV infection of ~2 months


title: Human Acute HIV Sample A144-CD4+ T cells


title: Human Acute HIV Sample A051-CD8+ T cells


title: Human Acute HIV Sample A044-CD4 T cells


title: H889(A)

description: lung cancer cell line

tag: Histology

tag: Age

tag: Sex

tag: Race


title: A673_siEF1

status: Public on Feb 14 2007

city: Paris

first: Olivier

last: Delattre

address: 26 rue d'Ulm

department: Genetics

country: France

institute: Institut Curie

description: Ewing A673 cell line transfected with specific siRNA directed against EWS/FLI-1.

hyb_protocol: According to Affymetrix Gene-Chip expression analysis technical manual, Affymetrix, Santa Clara, Calif.

source_name: Ewing A673 cell line

growth_protocol: Double transfected (Day0 and day4) with specific siRNA directed against EWS/FLI-1. Cells were harvested at day 9

extract_protocol: Total RNAs were isolated using the Trizol Reagent

tag: Ewing tumor cell line


title: SKNMC_shEF1_rep2

description: Ewing SK-N-MC cell line treated with shRNA lentivirus directed against EWS/FLI-1 for 3 days

scan_protocol: According to Affymetrix Gene-Chip expression analysis technical manual, Affymetrix, Santa Clara, Calif.

source_name: Ewing SK-N-MC cell line

growth_protocol: Treated with shRNA lentivirus directed against EWS/FLI-1 for 3 days


title: EW24_shEF1_rep1

description: Ewing EW24 cell line treated with shRNA lentivirus directed against EWS/FLI-1 for 3 days

source_name: Ewing EW24 cell line

label_protocol: According to Affymetrix Gene-Chip expression analysis technical manual, Affymetrix, Santa Clara, Calif.

treatment_protocol: Treated with shRNA lentivirus directed against EWS/FLI-1 for 3 days

tag: Ewing cell line


title: A673_siCT_rep2

description: Ewing A673 cell line transfected with scramble (control) siRNA

growth_protocol: Double transfected (Day0 and day4) with scramble (control) siRNA. Cells were harvested at day 9


title: EW24_shEF1_rep2

data_processing: MAS5 and GCRMA


title: SKNMC_shEF1_rep1


title: SKNMC_shCTL_rep1

description: Ewing SK-N-MC cell line treated with scramble (control) shRNA lentivirus for 3 days

growth_protocol: Treated with scramble (control) shRNA lentivirus for 3 days


title: P6M

status: Public on Sep 15 2006

city: Boston

first: Wenzhong Xiao

state: MA

address: 55 Fruit Street, GRV 1302

department: Department of Surgery

institute: Massachusetts General Hospital

description:
circulating whole white blood cells, isolated T cells and Monocytes
Data for Inflamation and Host Reponse to Injury, NIH Glue Grant, Study or Experiment

hyb_protocol: Standard Affymetrix procedures

scan_protocol: Standard Affymetrix procedures

data_processing: Affymetrix GCOS version 1.2

source_name: Patient 6 monocytes

tag: Human trauma patients with documented multiple organ failure and healthy volunteers


title: A673_siCT_rep1


title: P7T

source_name: Patient 7 T cells


title: P4M

source_name: Patient 4 monocytes


title: P2T

source_name: Patient 2 T cells


title: P5T

source_name: Patient 5 T cells


title: P3M

source_name: Patient 3 monocytes


title: P3L

source_name: Patient 3 leukocytes


title: P7L

source_name: Patient 7 leukocytes


title: P4L

source_name: Patient 4 leukocytes


title: P6T

source_name: Patient 6 T cells


title: P4T

source_name: Patient 4 T cells


title: EW24_shCTL_rep1

description: Ewing EW24 cell line treated with scramble (control) shRNA lentivirus for 3 days

treatment_protocol: Treated with scramble (control) shRNA lentivirus for 3 days


title: SKNMC_shCTL_rep2


title: P7M

source_name: Patient 7 monocytes


title: P6L

source_name: Patient 6 leukocytes


title: P5M

source_name: Patient 5 monocytes


title: P2M

source_name: Patient 2 monocytes


title: P1M

source_name: Patient 1 monocytes


title: P2L

source_name: Patient 2 leukocytes


title: P3T

source_name: Patient 3 T cells


title: P1T

source_name: Patient 1 T cells


title: P1L

source_name: Patient 1 leukocytes


title: C7M

source_name: Control 7 monocytes


title: C7L

source_name: Control 7 leukocytes


title: C7T

source_name: Control 7 T cells


title: C6T

source_name: Control 6 T cells


title: C5T

source_name: Control 5 T cells


title: C4T

source_name: Control 4 T cells


title: C4M

source_name: Control 4 monocytes


title: C5M

source_name: Control 5 monocytes


title: C4L

source_name: Control 4 leukocytes


title: C2L

source_name: Control 2 leukocytes


title: C3L

source_name: Control 3 leukocytes


title: C1T

source_name: Control 1 T cells


title: C2T

source_name: Control 2 T cells


title: C3M

source_name: Control 3 monocytes


title: C6M

source_name: Control 6 monocytes


title: C1M

source_name: Control 1 monocytes


title: C3T

source_name: Control 3 T cells


title: C6L

source_name: Control 6 leukocytes


title: C1L

source_name: Control 1 leukocytes


title: C5L

source_name: Control 5 leukocytes


title: HCC827(A)

status: Public on May 16 2006

source_name: lung cancer cell line

value: adenocarcinoma (BAC features)


title: C2M

source_name: Control 2 monocytes


title: HCC515(A)


title: HCC44(A)

tag: Age:


title: HCC366(A)

value: adenosquamous

tag: Race:


title: HCC95(A)

value: M


title: HCC78(A)


title: HCC2279(A)

value: asian


title: HCC461(A)


title: HCC2935(A)


title: HCC193(A)


title: HCC15(A2)


title: HCC4006(A)


title: HCC1171(A)


title: H460(A2)


title: H358(A)

value: Bronchoalveolar


title: HCC1195(A)

value: adenocarcinoma (mixed)


title: H441(A)

value: adenocarcinoma


title: H2887(A)


title: H1666(A)

value: F

value: caucasian


title: H1395(A)


title: H2347(A)


title: H1672(A2)

value: SCLC


title: H2122(A)


title: H1650(A)


title: H2009(A)


title: H1975(A)


title: H3255(A)


title: H2882(A)

value: NSCLC


title: H1648(A)

value: black


title: H820(A)


title: H2126(A)

value: large cell carcinoma


title: H157(A)

value: squamous cell carcinoma


title: H2087(A)


title: H1437(A)


title: H1355(A)


title: H1819(A)


title: HCC1359(A)


title: Calu-1(A)


title: Calu-3(A)


title: H1299(A)


title: A549(A)


title: H1993(A)


title: Calu-6(A)


title: Th cells_antiCD3+antiCD28+IL12_2h_replicate 2a (U133A)

status: Public on Mar 22 2006

city: Turku

first: Riikka

middle: Johanna

last: Lund

address: Tykistökatu 6 A

department: Turku Centre for Biotechnology

country: Finland

institute: University of Turku and Åbo Akademi University

source_name: Cord blood CD4+ cells

tag: CD4+ cells isolated from randomly collected cord bloods of healthy neonates, cells pooled from different individuals


title: Th cells_antiCD3+antiCD28+IL12+TGFbeta_48h_replicate 2b (U133A)


title: Th cells_antiCD3+antiCD28_48h_replicate 2b (U133A)


title: Th cells_antiCD3+antiCD28_48h_replicate 1b (U133A)


title: Th cells_no treatment_0h_replicate 2b (U133A)


title: Th cells_antiCD3+antiCD28+IL12_48h_replicate 2b (U133A)


title: Th cells_antiCD3+antiCD28+IL12_6h_replicate 2a (U133A)


title: Th cells_no treatment_0h_replicate 1b (U133A)


title: Th cells_antiCD3+antiCD28+IL12+TGFbeta_6h_replicate 2a (U133A)


title: Th cells_no treatment_0h_replicate 2a (U133A)


title: Th cells_antiCD3+antiCD28+IL12+TGFbeta_2h_replicate 2a (U133A)


title: Th cells_antiCD3+antiCD28+IL4_6h_replicate 2a (U133A)


title: Th cells_antiCD3+antiCD28+IL4_6h_replicate 1a (U133A)


title: Th cells_antiCD3+antiCD28_6h_replicate 2a (U133A)


title: Th cells_antiCD3+antiCD28+IL4+TGFbeta_48h_replicate 2b (U133A)


title: Th cells_antiCD3+antiCD28+IL12_6h_replicate 1a (U133A)


title: Th cells_antiCD3+antiCD28+IL4+TGFbeta_2h_replicate 2a (U133A)


title: Th cells_antiCD3+antiCD28+IL4_48h_replicate 2b (U133A)


title: Th cells_antiCD3+antiCD28+IL4+TGFbeta_48h_replicate 1b (U133A)


title: Th cells_antiCD3+antiCD28_2h_replicate 2a (U133A)


title: Th cells_antiCD3+antiCD28+IL4_2h_replicate 1a (U133A)


title: Th cells_antiCD3+antiCD28+IL4_2h_replicate 2a (U133A)


title: Th cells_antiCD3+antiCD28+IL4+TGFbeta_6h_replicate 2a (U133A)


title: Th cells_antiCD3+antiCD28+IL4+TGFbeta_2h_replicate 1a (U133A)


title: Th cells_antiCD3+antiCD28_2h_replicate 1a (U133A)


title: Th cells_antiCD3+antiCD28+IL4_48h_replicate 1b (U133A)


title: Th cells_antiCD3+antiCD28+IL12+TGFbeta_48h_replicate 1b (U133A)


title: Th cells_antiCD3+antiCD28+IL12_48h_replicate 1b (U133A)


title: Th cells_antiCD3+antiCD28+IL12+TGFbeta_2h_replicate 1a (U133A)


title: Th cells_antiCD3+antiCD28+IL12+TGFbeta_6h_replicate 1a (U133A)


title: Th cells_antiCD3+antiCD28_6h_replicate 1a (U133A)


title: Th cells_no treatment_0h_replicate 1a (U133A)


title: Th cells_antiCD3+antiCD28+IL4+TGFbeta_6h_replicate 1a (U133A)


title: poly2

status: Public on Jan 30 2004

city: Cambridge

first: Ken

last: Ross

address: 7 Cambridge Center

department: Cancer Genomics

institute: Broad Institute

description:
Normal peripheral blood neutrophils isolated using Ficoll-Paque separation from a donor leukopack discard sample provided by the Dana Farber Cancer Institute Blood Bank. RNA extraction, target preparation, and hybridization as described in Stegmaier et al., Nature Genetics 2004.
Keywords = neutrophil

source_name: normal human neutrophils


title: mono2

description:
Normal peripheral blood monocytes isolated using Ficoll-Paque separation from a donor leukopack discard sample provided by the Dana Farber Cancer Institute Blood Bank. RNA extraction, target preparation, and hybridization as described in Stegmaier et al., Nature Genetics 2004.
Keywords = monocyte

source_name: nomral human monocytes


title: poly1


title: mono1

source_name: normal human monocytes


title: Th cells_antiCD3+antiCD28+IL12_2h_replicate 1a (U133A)