description:
Biological replicate 1 of 2. Med7 occupancy in med13D cells treated with 6 microM NAPP1 for 15 minutes. Med7 ChIP sample (Cy5-labeled) was performed from a 13xMYC-tagged Med7 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED7 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med7 occupancy in med13D cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR2355vsFR2107_Med7-13MYCvsNoTag_KIN28med13D_15minNAPP1_20160406_rep1-2__S.cerevisiaeApr11_35740.bed;

growth_protocol: 60mL YEP+2% Glucose containing about 15 glass beads of 4mm-diameter is inoculated at OD600 0.15 from an overnight preculture in the appropriate media, agitated at 330rpm and allowed to grow until it reaches OD600 0.6-0.8.  NA-PP1 is added at final concentration 6uM and cultures allowed to grow for an additional 15 min.  Culture is poured in 50mL Falcon tube containing 1.4mL 37% formaldehyde for crosslinking, and incubate on rotating wheel for 30 min. Crosslinking is stopped by addition of 2.5mL 2.5M Glycine to quench formaldehyde (optional). Crosslinked cells are pelleted at 4'C, 3000 rpm, resuspended in 40mL ice cold TBS and pelleted again. This wash is repeated a second time. Cell pellets are resuspended in the remaining liquid (~1mL) and transfered to 1.5mL screw cap tubes. Cells are pelleted by centrifugation, the supernatant removed by pipetting, and the cells pellet snap-freeze in liquid nitrogen and stock at -80'C. Ref: Holstege lab, van de Peppel et al., Molecular Cell 2005.

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 med13Δ::kanMX4 MED7-13xMYC::HPHr

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 med13Δ::KanMX4


title: 4113

status: Public on Oct 25 2016

type: genomic

city: Seattle

first: William

middle: M

last: Grady

state: WASHINGTON

address: 1100 Fairview N.Ave

department: Department of Clinical Research

country: USA

institute: Fred Hutchinson Cancer Research Center

description: esophagus sample

hyb_protocol: per Illumina Infinium protocol

scan_protocol: per Illumina Infinium protocol

data_processing:
Filtered out probes with detection p-value > 0.05, probes on the X chromosome, and probes containing at least one SNP with low minor allele frequency (MAF=0) in the probe body. The background noise is corrected and SWAN normalization is performed by using the functions provided by the Bioconductor minfi package. Batch effects across three batch runs are corrected using the Combat algorithm from the R sva package.
MatrixSignalIntensities.csv: Unmethylated and methylated signal intensities and detection p-value

label: Cy5 and Cy3

molecule: genomic DNA

organism: Homo sapiens

source_name: esophagus

label_protocol: per Illumina Infinium protocol

extract_protocol: formalin-fixed, paraffin-embedded samples were extracted using the DNAeasy blood and tissue kit (Qiagen)

tag: run

tag: histology

value: EAC

tag: age

tag: gender

value: M

tag: Stage

value: I

tag: smokecigarettes

value: Yes

tag: alcoholicrinksperweek

tag: be status


title: 4106

description: esophagus sample

value: LGD

tag: bmi

value: III


title: yFR2356vsFR2108_Med7-13MYCvsNoTag_kin28asmed13D_15minNAPP1_20160406_rep2

city: Montreal

first: Francois

last: Robert

state: QC

address: 110 av des Pins Ouest

country: Canada

institute: IRCM

description:
Biological replicate 2 of 2. Med7 occupancy in kin28asmed13D cells treated with 6 microM NAPP1 for 15 minutes. Med7 ChIP sample (Cy5-labeled) was performed from a 13xMYC-tagged Med7 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED7 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med7 occupancy in kin28asmed13D cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR2356vsFR2108_Med7-13MYCvsNoTag_kin28asmed13D_15minNAPP1_20160406_rep1-2__S.cerevisiaeApr11_780120.bed;

hyb_protocol: Hybridization: Resuspend colored pellet in 110uL hybridization buffer (100uL DIGEasy Buffer, 5uL 10mg/mL Salmon Sperm DNA, 5uL 8mg/mL yeast tRNA). Put at 95?C for 5 minutes. Put at 42?C for 10 min (Keep samples at 42?C while waiting for hybridization). Follow standard Agilent chamber hybridization protocols. Incubate overnight (16-24h) at 42?C in Agilent hybridization oven at 20 rpm rotation speed.  Post-Hybridization Washes: Separate coverslip and slide in Wash buffer #1. Wash slides in Wash buffer #1 for 5 minutes at room temperature on an orbital shaker (cover the dish to avoid light on the slides). Wash in 31C Wash buffer #2 for 5 minutes on an orbital shaker. Take slides out of the buffer slowly to dry them. Wash Buffer #1: 6x SSPE, 0.005% N-lauroylsarcosine. Wash Buffer #2: 0.06x SSPE.  Stripping: Stripping is done in 1L of 5 mM Potassium phosphate buffer pH 6.6 after scanning of the slides. 1. Use a crystallization dish, and a metallic slide rack (use weighing spatulas to lift the rack from the bottom of the dish and avoid direct contact with the hot surface). Pour the stripping buffer, submerge the rack with slides (from 1 to 10) and heat it up slowly on heating plate until the liquid boils with big bubbles. It usually takes 15 to 20 minutes. Do not overboil it. 2. Remove the rack and briefly rinse in a container with water. Slowly remove the rack from the liquid. Cy5 channel will be stripped much better than Cy3, but this does not affect the performance of subsequent hybridizations.  Stock solution of 1M Potassium phosphate buffer, pH 6.6: Mix 3.81 ml of 1M K2HPO4 + 6.19 ml of 1M KH2PO4. Dilute 5 ml of this 1M solution in 1L Milli-Q water to obtain 5mM Potassium phosphate buffer, pH 6.6.

scan_protocol: Standard_Innopsys_Yeast4x180K  Set the scan as follow:  pixel size = 2Âµm;  speed = 20;  Scan Mode = Normal;  Acquisition Mode = Simultaneous;  Focus = Auto.    Adjust manually the detection gain for 635 and 532 wavelengths in order to have less than 5% spot saturation.

data_processing:
Correction = Foreground-Background  Normalisation = limma loess (subgrid)
Bed files: The ChIP-chip data from individual replicates were normalized using the Limma Loess method and replicates were combined as described previously (Jeronimo and Robert, 2014, Nat Struct Mol Biol 21(5):449-55). The data were subjected to one round of smoothing using a Gaussian sliding window with a standard deviation of 100 bp to generate data points in 10 bp intervals as described before (Guillemette et al., 2005, PLoS Biol 3(12):e384).

organism: Saccharomyces cerevisiae

label_protocol: Blunting: Do everything on ice! Add 70uL of the blunting solution (11uL NEB Buffer #2, 0.5uL BSA 10mg/mL, 0.5uL 20mM dNTPs, 0.2uL T4 DNA Polymerase 3U/uL, 57.8uL ddH2O) to 40uL of the resuspended DNA obtained at the end of the cleanup step. For WCEs, use 2uL + 38uL ddH2O instead of 40uL. Incubate 20 minutes at 12C. Add 11.5uL 3M NaOAc, pH 5.2, and 0.5uL Glycogen 20mg/mL and vortex briefly. Extract with 120uL Phenol/Chloroform/Isoamyl alcohol (25:24:1). Transfer 110uL to a new tube, and add 230uL -20C 100% EtOH. Put at -80C for 30min, then spin 20 minutes at 4C. Wash the pellet with 500uL -20C 70% EtOH and spin 15 minutes at 4C. Resuspend the pellet in 25uL ddH2O and place on ice. Ligation: Thaw everything on ice, and do all manipulations on ice. Add 25uL of ligase mix (10uL 5x T4 Ligase Buffer, 6.7uL 15uM unidirectional linkers, 0.5uL T4 DNA Ligase, High Concentration, 8uL ddH2O) to the resuspended pellet from the blunting step. Incubate overnight at 16C. Add 6uL 3M NaOAc, pH 5.2, and vortex. Add 130uL -20C 100% EtOH and centrifuge 20 minutes at 4C. Wash the pellet (should be a smear on the side of the tube) with 500uL -20C 70% EtOH. Spin 15 minutes at 4C. Dry the pellet and resuspend in 25uL ddH2O.    Labeling: Add 15uL of PCR Labeling mix (4uL 10x ThermoPol Buffer, 2uL 5mM aa-dUTP mix, 1.25uL Oligo FR1, 7.75uL ddH2O) to the pellet from the ligation step. Start the PCR program, and during the first step (4 minutes at 55C), add 10uL of the polymerase mix (1uL 10x ThermoPol buffer, 1uL Taq Polymerase 5U/uL, 0.01uL Pfu Polymerase Turbo 2.5U/uL, 8uL ddH2O).  PCR cycling conditions:  1) 5:00 at 55C  2) 5:00 at 72C  3) 2:00 at 95C  4) 0:30 at 95C  5) 0:30 at 55C  6) 1:00 at 72C  7) Repeat steps 4-6 31 more times.  8) 4:00 at 72C  9) Forever at 4C    Purify PCR reactions with Qiagen PCR Cleanup kits, following their protocol BUT use a phosphate wash buffer and a phosphate elution buffer instead of their buffers PE and EB, respectively. Also, wash the columns only once, and elute twice with 30uL elution buffer. Speed-vac the eluate to dryness.    Resuspend the pellets with fresh 4.5uL 0.1M Sodium Carbonate Buffer, pH 9.0. Add 4.5uL of the appropriate NHS-ester Cy-dye. Fresh tubes of NHS-ester Cy-dye should be resuspended in 73uL DMSO. Mix well, and incubate at room temperature in the dark for one hour.    Add 35uL 0.1M NaOAc, pH 5.2. Purify using the Qiagen PCR Cleanup protocol using all their buffers, but wash only once and elute twice in 30uL. It is a good idea to elute both samples of a pair (Cy5 and Cy3) in the same tube to avoid DNA loss upon later resuspension. Speed-vac until 10-20uL are left, or to dryness.      Phosphate Wash Buffer (100mL):  0.5mL 1M KPO4, pH 8.5  15.25mL ddH2O  84.25mL 95% EtOH  Solution will be cloudy upon mixing.    Phosphate Elution Buffer (10mL):  0.04mL 1M KPO4, pH 8.5  9.96mL ddH2O    1M KPO4, pH 8.5 (10mL):  9.5mL 1M K2HPO4  0.5mL 1M KH2PO4    1M Sodium Carbonate Buffer, pH 9.0 (100mL):  10.8g Na2CO3  80mL ddH2O  pH to 9.0 with concentrated HCl  Adjust volume to 100mL with ddH2O  DILUTE 1:10 WHEN USING!  Solution will change composition over time. Use only if less than a month old.

extract_protocol: Cells are thawed on ice, and resuspended in 700uL lysis buffer with protease inhibitors (solution composition detailed at the end). 500uL of acid-washed glass beads (Sigma) are added, and the cells are lysed using the bead-beater (4x5min cycles, with 5min breaks on ice). The tubes are pierced with a 18-gauge syringe needle to allow transfer of lysate, but not beads. The pierced tubes are put in 2mL collection tubes and spun briefly. The lysate is then homogenized by pipetting and sonicated 4x 20sec at output 7 Watts, with a 1 minute break between sonication cycles. The sonicated lysates is centrifuge for 5 min at 4'C max speed and supernatant used immediately for ChIP (or saved and frozen at -80'C). Lysis buffer: 50mM HEPES-KOH, pH 7.5 140mM NaCl 1mM EDTA 1% Triton X-100 0.1% Na-deoxycholate 1mM PMSF 1mM Benzamidine 10ug/mL Aprotinin 1ug/mL Leupeptin 1ug/mL Pepstatin.

treatment_protocol:
6microM NA-PP1 for 15 min
IP protocol: anti-MYCtag (9E10 lab stock 2015). Anti-mouse Dynabeads (50uL / ChIP) are washed twice with 10mL PBS + 0.5% BSA (freshly made), and resuspended in 1mL PBS + 0.5% BSA. 9E10 anti-Myc antibody (home-prepared, 2015)  is added (2.5uL / ChIP), and incubated overnight at 4'C in haematological mixer. Beads are washed twice with 10mL PBS + 0.5% BSA (freshly made), and resuspended in 30uL PBS + 0.5% BSA / ChIP. A mix of 250uL of cell lysate + 250uL of Lysis Buffer containing protease inhibitors are added to 30uL beads, and incubated overnight at 4'C in haematological mixer. Beads are then washed (using the Dynal magnet system) twice with 1mL ice cold Lysis Buffer (no protease inhibitors), twice with 1mL ice cold Lysis Buffer (no protease inhibitors) + additional 360mM NaCl (720uL of 5M NaCl per 10mL lysis buffer), twice with ice cold Wash Buffer, and once with ice cold TE. Remaining liquid is removed by pipetting, and 50uL TE + 1% SDS is added. Beads are incubated overnight at 65'C to reverse the crosslinks. Lysis buffer: 50mM HEPES-KOH, pH 7.5 140mM NaCl 1mM EDTA 1% Triton X-100 0.1% Na-deoxycholate 1mM PMSF 1mM Benzamidine 10ug/mL Aprotinin 1ug/mL Leupeptin 1ug/mL. Pepstatin.

tag: strain

tag: strain information

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 kin28::kin28as (L83G) [pSH579, CEN URA3 kin28as (L83G)] med13Δ::kanMX4 MED7-13xMYC::HPHr

tag: antibody

value: 9E10 (lab stock prepared by Pierre, 2015)

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 kin28::kin28as (L83G) [pSH579, CEN URA3 kin28as (L83G)] med13Δ::KanMX4


title: 4108

description: esophagus sample

value: Fundus


title: 3990

description: esophagus sample

value: BE

value: F

value: No

value: Less than one drink a week


description:
Biological replicate 1 of 2. Med7 occupancy in kin28asmed13D cells treated with 6 microM NAPP1 for 15 minutes. Med7 ChIP sample (Cy5-labeled) was performed from a 13xMYC-tagged Med7 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED7 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med7 occupancy in kin28asmed13D cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR2356vsFR2108_Med7-13MYCvsNoTag_kin28asmed13D_15minNAPP1_20160406_rep1-2__S.cerevisiaeApr11_780120.bed;


title: yFR2355vsFR2107_Med7-13MYCvsNoTag_KIN28med13D_15minNAPP1_20160406_rep2

description:
Biological replicate 2 of 2. Med7 occupancy in med13D cells treated with 6 microM NAPP1 for 15 minutes. Med7 ChIP sample (Cy5-labeled) was performed from a 13xMYC-tagged Med7 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED7 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med7 occupancy in med13D cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR2355vsFR2107_Med7-13MYCvsNoTag_KIN28med13D_15minNAPP1_20160406_rep1-2__S.cerevisiaeApr11_35740.bed;


title: 4030

description: esophagus sample

value: Cardia


description:
Biological replicate 1 of 2. Med7 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes. Med7 ChIP sample (Cy5-labeled) was performed from a 13xMYC-tagged Med7 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED7 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med7 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1900vsFR762-KIN28_Med7-13MYCvsUntagged_15minNAPP1_20160129_rep1-2__S.cerevisiaeApr11_131621.bed;

growth_protocol: YEP+2% Glucose is inoculated at OD600 0.1 from an overnight preculture in the appropriate media and allowed to grow until it reach OD600 0.6-0.8. NA-PP1 is added at final concentration 6uM and cultures allowed to grow for an additional 15 min. Culture is poured/divided in 50mL Falcon tube containing 1.4mL 37% formaldehyde for crosslinking, and incubate on rotating wheel for 30 min. Crosslinking is stopped by addition of 2.5mL 2.5M Glycine to quench formaldehyde. Crosslinked cells are pelleted at 4'C, 3000 rpm, resuspended in 40mL ice cold TBS and pelleted again. This wash is repeated a second time. Cell pellets are resuspended in the remaining liquid (~1mL) and transfered to 1.5mL screw cap tubes. Cells are pelleted by centrifugation, the supernatant removed by pipetting, and the cells pellet snap-freeze in liquid nitrogen and stock at -80'C

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 MED7-13xMYC::HPHr


title: yFR1902vsFR763_Med7-13MYCvsNoTag_kin28as_15minNAPP1_20160406_rep2

description:
Biological replicate 2 of 2. Med7 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes. Med7 ChIP sample (Cy5-labeled) was performed from a 13xMYC-tagged Med7 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED7 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med7 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1902vsFR763_Med7-13MYCvsNoTag_kin28as_15minNAPP1_20160406_rep1-2__S.cerevisiaeApr11_577886.bed;

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 kin28::kin28as (L83G) [pSH579, CEN URA3 kin28as (L83G)] MED7-13xMYC::HPHr


description:
Biological replicate 1 of 2. Med15 occupancy in kin28asmed13D cells treated with 6 microM NAPP1 for 15 minutes. Med15 ChIP sample (Cy5-labeled) was performed from a 9xMYC-tagged Med15 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED15 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med15 occupancy in kin28asmed13D cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR2109vsFR2108_Gal11-9MYCvsNoTag_kin28asmed13D_15minNAPP1_20160331_rep1-2__S.cerevisiaeApr11_61216.bed;

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 kin28::kin28as (L83G) [pSH579, CEN URA3 kin28as (L83G)] med13Δ::KanMX4 MED15-9xMYC::klTRP1


title: 4109

description: esophagus sample

value: HGD

value: M


title: yFR1902vsFR763-kin28as_Med7-13MYCvsUntagged_15minNAPP1_20160129_rep2

description:
Biological replicate 2 of 2. Med7 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes. Med7 ChIP sample (Cy5-labeled) was performed from a 13xMYC-tagged Med7 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED7 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med7 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1902vsFR763-kin28as_Med7-13MYCvsUntagged_15minNAPP1_20160129_rep1-2__S.cerevisiaeApr11_407586.bed;

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 kin28::kin28as (L83G) [pSH579, CEN URA3 kin28as (L83G)]


title: yFR2109vsFR2108_Gal11-9MYCvsNoTag_kin28asmed13D_15minNAPP1_20160331_rep2

description:
Biological replicate 2 of 2. Med15 occupancy in kin28asmed13D cells treated with 6 microM NAPP1 for 15 minutes. Med15 ChIP sample (Cy5-labeled) was performed from a 9xMYC-tagged Med15 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED15 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med15 occupancy in kin28asmed13D cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR2109vsFR2108_Gal11-9MYCvsNoTag_kin28asmed13D_15minNAPP1_20160331_rep1-2__S.cerevisiaeApr11_61216.bed;


title: 4107

description: esophagus sample

value: EAC


title: 4105

description: esophagus sample

value: Yes


description:
Biological replicate 1 of 2. Med7 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes. Med7 ChIP sample (Cy5-labeled) was performed from a 13xMYC-tagged Med7 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED7 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med7 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1902vsFR763_Med7-13MYCvsNoTag_kin28as_15minNAPP1_20160406_rep1-2__S.cerevisiaeApr11_577886.bed;


description:
Biological replicate 1 of 2. Med15 occupancy in med13D cells treated with 6 microM NAPP1 for 15 minutes. Med15 ChIP sample (Cy5-labeled) was performed from a 9xMYC-tagged Med15 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED15 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med15 occupancy in med13D cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR2102vsFR2107_Gal11-9MYCvsNoTag_KIN28med13D_15minNAPP1_20160331_rep1-2__S.cerevisiaeApr11_217747.bed;

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 med13Δ::KanMX4 MED15-9xMYC::klTRP1


title: yFR2362vs2274_Srb10-Cdk8-9MYC_med3Dmed15D_MYCvsNotag_20160406_rep2

description:
Biological replicate 2 of 2. Cdk8 occupancy in med3Dmed15D cells. Cdk8 ChIP sample (Cy5-labeled) was performed from a 9xMYC-tagged Cdk8 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the CDK8 gene is not tagged (Cy3-labeled).
genome browser-ready file for Cdk8 occupancy in med3Dmed15D cells
processed data file: SmthBED_yFR2362vs2274_Srb10-Cdk8-9MYC_med3Dmed15D_MYCvsNotag_20160406_rep1-2__S.cerevisiaeApr11_588100.bed;

growth_protocol: 50mL YEP+2% Glucose is inoculated at OD600 0.1 from an overnight preculture in the appropriate media and allowed to grow until it reach OD600 0.6-1.0. Culture is poured in 50mL Falcon tube containing 1.4mL 37% formaldehyde for crosslinking, and incubate on rotating wheel for 30 min. Crosslinking is stopped by addition of 2.5mL 2.5M Glycine to quench formaldehyde (optional). Crosslinked cells are pelleted at 4'C, 3000 rpm, resuspended in 40mL ice cold TBS and pelleted again. This wash is repeated a second time. Cell pellets are resuspended in the remaining liquid (~1mL) and transfered to 1.5mL screw cap tubes. Cells are pelleted by centrifugation, the supernatant removed by pipetting, and the cells pellet snap-freeze in liquid nitrogen and stock at -80'C

treatment_protocol:
null
IP protocol: anti-MYCtag (9E10 lab stock 2015). Anti-mouse Dynabeads (50uL / ChIP) are washed twice with 10mL PBS + 0.5% BSA (freshly made), and resuspended in 1mL PBS + 0.5% BSA. 9E10 anti-Myc antibody (home-prepared, 2015)  is added (2.5uL / ChIP), and incubated overnight at 4'C in haematological mixer. Beads are washed twice with 10mL PBS + 0.5% BSA (freshly made), and resuspended in 30uL PBS + 0.5% BSA / ChIP. A mix of 250uL of cell lysate + 250uL of Lysis Buffer containing protease inhibitors are added to 30uL beads, and incubated overnight at 4'C in haematological mixer. Beads are then washed (using the Dynal magnet system) twice with 1mL ice cold Lysis Buffer (no protease inhibitors), twice with 1mL ice cold Lysis Buffer (no protease inhibitors) + additional 360mM NaCl (720uL of 5M NaCl per 10mL lysis buffer), twice with ice cold Wash Buffer, and once with ice cold TE. Remaining liquid is removed by pipetting, and 50uL TE + 1% SDS is added. Beads are incubated overnight at 65'C to reverse the crosslinks. Lysis buffer: 50mM HEPES-KOH, pH 7.5 140mM NaCl 1mM EDTA 1% Triton X-100 0.1% Na-deoxycholate 1mM PMSF 1mM Benzamidine 10ug/mL Aprotinin 1ug/mL Leupeptin 1ug/mL. Pepstatin.

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 med3Δ::LEU2 med15Δ::kanMX4 MED7::HIS3 CDK8-9xMYC::klTRP1

value: 9E10 (lab stock prepared by Pierre, 2015)


title: yFR2287vsFR2263-med13D_Srb10-Cdk8-3FLAGvsNotag_20160202_rep2

description:
Biological replicate 2 of 2. Cdk8 occupancy in med13D cells. Cdk8 ChIP sample (Cy5-labeled) was performed from a 3xFLAG-tagged Cdk8 strain using the anti-FLAG antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the CDK8 gene is not tagged (Cy3-labeled).
genome browser-ready file for Cdk8 occupancy in med13D cells
processed data file: SmthBED_yFR2287vsFR2263-med13D_Srb10-Cdk8-3FLAGvsNotag_20160202_rep1-2__S.cerevisiaeApr11_16218.bed;

growth_protocol: 60mL YEP+2% Glucose containing about 15 glass beads of 4mm-diameter is inoculated at OD600 0.15 from an overnight preculture in the appropriate media, agitated at 330rpm and allowed to grow until it reaches OD600 0.6-0.8. Culture is poured in 50mL Falcon tube containing 1.4mL 37% formaldehyde for crosslinking, and incubate on rotating wheel for 30 min. Crosslinking is stopped by addition of 2.5mL 2.5M Glycine to quench formaldehyde (optional). Crosslinked cells are pelleted at 4'C, 3000 rpm, resuspended in 40mL ice cold TBS and pelleted again. This wash is repeated a second time. Cell pellets are resuspended in the remaining liquid (~1mL) and transfered to 1.5mL screw cap tubes. Cells are pelleted by centrifugation, the supernatant removed by pipetting, and the cells pellet snap-freeze in liquid nitrogen and stock at -80'C. Ref: Holstege lab, van de Peppel et al., Molecular Cell 2005.

treatment_protocol:
null
IP protocol: Flag_M2_SigmaF3165. Anti-mouse Dynabeads (PanMouse) (50uL / ChIP) are washed twice with PBS + 0.5% BSA (freshly made), and resuspended in PBS + 0.5% BSA. Anti-Flag M2 (Sigma F3165) is added (5ug/ChIP), and incubated overnight at 4'C in haematological mixer. Beads are washed twice (with PBS/BSA or Lysis buffer), and resuspended in 30uL (PBS/BSA or Lysis buffer) / ChIP. 30uL of resuspended beads/antibody is added to 500 uL of lysate, and incubated overnight at 4'C in haematological mixer. Beads are then washed in cold room (using the Dynal magnet system) twice with 1mL ice cold Lysis Buffer (no protease inhibitors), twice with 1mL ice cold Lysis Buffer (no protease inhibitors) + additional 360mM NaCl, twice with ice cold Wash Buffer, and once with ice cold TE. Remaining liquid is removed by pipetting after a brief centrifugation at 4'C, and 50uL TE + 1% SDS is added for elution. Beads are incubated overnight at 65'C for elution and reverse crosslink. Note for Whole Cell Extracts (WCE): WCE are included in the protocol only at the crosslink reversal step. Add 50uL TE + 1% SDS to 5uL WCE, and put overnight at 65'C to reverse the crosslinks with the ChIP samples. Lysis Buffer (no protease inhibitors): 50mM HEPES-KOH, pH 7.5 140mM NaCl 1mM EDTA 1% Triton X-100 0.1% Na-deoxycholate Wash Buffer: 10mM Tris-HCl, pH 8.0 250mM LiCl 0.5% NP40 0.5% Na-deoxycholate 1mM EDTA.

value: MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 med13Δ::LEU2 CDK8-3xFLAG::kanMX

value: Flag M2 R (Sigma)


title: yFR1636vsFR762_Gal11-9MYCvsNoTag_KIN28_15minNAPP1_20160331_rep2

description:
Biological replicate 2 of 2. Med15 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes. Med15 ChIP sample (Cy5-labeled) was performed from a 9xMYC-tagged Med15 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED15 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med15 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1636vsFR762_Gal11-9MYCvsNoTag_KIN28_15minNAPP1_20160331_rep1-2__S.cerevisiaeApr11_399502.bed;

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 MED15-9xMYC::klTRP1


description:
Biological replicate 1 of 2. Cdk8 occupancy in med13D cells. Cdk8 ChIP sample (Cy5-labeled) was performed from a 3xFLAG-tagged Cdk8 strain using the anti-FLAG antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the CDK8 gene is not tagged (Cy3-labeled).
genome browser-ready file for Cdk8 occupancy in med13D cells
processed data file: SmthBED_yFR2287vsFR2263-med13D_Srb10-Cdk8-3FLAGvsNotag_20160202_rep1-2__S.cerevisiaeApr11_16218.bed;

value: MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 med13Δ::LEU2


title: yFR1638vsFR763_Gal11-9MYCvsNoTag_kin28as_15minNAPP1_20160331_rep2

description:
Biological replicate 2 of 2. Med15 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes. Med15 ChIP sample (Cy5-labeled) was performed from a 9xMYC-tagged Med15 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED15 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med15 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1638vsFR763_Gal11-9MYCvsNoTag_kin28as_15minNAPP1_20160331_rep1-2__S.cerevisiaeApr11_614092.bed;

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 kin28::kin28as (L83G) MED15-9xMYC::klTRP1 [pSH579, CEN URA3 kin28as (L83G)]


title: yFR2286vsFR117-WT_Srb10-Cdk8-3FLAGvsNoTag_20160202_rep2

description:
Biological replicate 2 of 2. Cdk8 occupancy in WT cells. Cdk8 ChIP sample (Cy5-labeled) was performed from a 3xFLAG-tagged Cdk8 strain using the anti-FLAG antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the CDK8 gene is not tagged (Cy3-labeled).
genome browser-ready file for Cdk8 occupancy in WT cells
processed data file: SmthBED_yFR2286vsFR117-WT_Srb10-Cdk8-3FLAGvsNoTag_20160202_rep1-2__S.cerevisiaeApr11_160525.bed;

value: MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 CDK8-3xFLAG::kanMX

value: MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0


title: yFR2187vsFR762-WT_15minNAPP1_Med6-18MYCvsNoTag_20151012_rep4

description:
Biological replicate 2 of 2. Med6 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes. Med6 ChIP sample (Cy5-labeled) was performed from a 18xMYC-tagged Med6 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED6 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med6 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR2187vsFR762-WT_15minNAPP1_Med6-18MYCvsNoTag_20151012_rep3-4__S.cerevisiaeApr11_898611.bed;

treatment_protocol:
6microM NA-PP1 for 15 min
IP protocol: anti-MYCtag (9E10 lab stock). Anti-mouse Dynabeads (50uL / ChIP) are washed twice with 10mL PBS + 0.5% BSA (freshly made), and resuspended in 1mL PBS + 0.5% BSA. 9E10 anti-Myc antibody (home-prepared)  is added (10uL / ChIP), and incubated overnight at 4'C in haematological mixer. Beads are washed twice with 10mL PBS + 0.5% BSA (freshly made), and resuspended in 30uL PBS + 0.5% BSA / ChIP. A mix of 250uL of cell lysate + 250uL of Lysis Buffer containing protease inhibitors are added to 30uL beads, and incubated overnight at 4'C in haematological mixer. Beads are then washed (using the Dynal magnet system) twice with 1mL ice cold Lysis Buffer (no protease inhibitors), twice with 1mL ice cold Lysis Buffer (no protease inhibitors) + additional 360mM NaCl (720uL of 5M NaCl per 10mL lysis buffer), twice with ice cold Wash Buffer, and once with ice cold TE. Remaining liquid is removed by pipetting, and 50uL TE + 1% SDS is added. Beads are incubated overnight at 65'C to reverse the crosslinks. Lysis buffer: 50mM HEPES-KOH, pH 7.5 140mM NaCl 1mM EDTA 1% Triton X-100 0.1% Na-deoxycholate 1mM PMSF 1mM Benzamidine 10ug/mL Aprotinin 1ug/mL Leupeptin 1ug/mL. Pepstatin.

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 MED6-18xMYC::klTRP1

value: 9E10 (lab stock by AF)


description:
Biological replicate 1 of 2. Med6 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes. Med6 ChIP sample (Cy5-labeled) was performed from a 18xMYC-tagged Med6 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED6 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med6 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR2187vsFR762-WT_15minNAPP1_Med6-18MYCvsNoTag_20151012_rep3-4__S.cerevisiaeApr11_898611.bed;

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0


description:
Biological replicate 1 of 2. Cdk8 occupancy in med3Dmed15D cells. Cdk8 ChIP sample (Cy5-labeled) was performed from a 9xMYC-tagged Cdk8 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the CDK8 gene is not tagged (Cy3-labeled).
genome browser-ready file for Cdk8 occupancy in med3Dmed15D cells
processed data file: SmthBED_yFR2362vs2274_Srb10-Cdk8-9MYC_med3Dmed15D_MYCvsNotag_20160406_rep1-2__S.cerevisiaeApr11_588100.bed;

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 med3Δ::LEU2 med15Δ::kanMX4 MED7::HIS3


description:
Biological replicate 1 of 2. Med15 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes. Med15 ChIP sample (Cy5-labeled) was performed from a 9xMYC-tagged Med15 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED15 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med15 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1636vsFR762_Gal11-9MYCvsNoTag_KIN28_15minNAPP1_20160331_rep1-2__S.cerevisiaeApr11_399502.bed;


description:
Biological replicate 1 of 2. Cdk8 occupancy in WT cells. Cdk8 ChIP sample (Cy5-labeled) was performed from a 9xMYC-tagged Cdk8 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the CDK8 gene is not tagged (Cy3-labeled).
genome browser-ready file for Cdk8 occupancy in WT cells
processed data file: SmthBED_yFR1685vs762_Srb10-Cdk8-9MYC_WT_MYCvsNotag_20160406_rep1-2__S.cerevisiaeApr11_12746.bed;

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 CDK8-9xMYC::klTRP1


title: yFR2188vsFR762-WT_15minNAPP1_Med16-Sin4-18MYCvsNoTag_20151012_rep4

description:
Biological replicate 2 of 2. Med16 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes. Med16 ChIP sample (Cy5-labeled) was performed from a 18xMYC-tagged Med16 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED16 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med16 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR2188vsFR762-WT_15minNAPP1_Med16-Sin4-18MYCvsNoTag_20151012_rep3-4__S.cerevisiaeApr11_871273.bed;

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 MED16-18xMYC::klTRP1


description:
Biological replicate 1 of 2. Cdk8 occupancy in WT cells. Cdk8 ChIP sample (Cy5-labeled) was performed from a 3xFLAG-tagged Cdk8 strain using the anti-FLAG antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the CDK8 gene is not tagged (Cy3-labeled).
genome browser-ready file for Cdk8 occupancy in WT cells
processed data file: SmthBED_yFR2286vsFR117-WT_Srb10-Cdk8-3FLAGvsNoTag_20160202_rep1-2__S.cerevisiaeApr11_160525.bed;


title: yFR2102vsFR2107_Gal11-9MYCvsNoTag_KIN28med13D_15minNAPP1_20160331_rep2

description:
Biological replicate 2 of 2. Med15 occupancy in med13D cells treated with 6 microM NAPP1 for 15 minutes. Med15 ChIP sample (Cy5-labeled) was performed from a 9xMYC-tagged Med15 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED15 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med15 occupancy in med13D cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR2102vsFR2107_Gal11-9MYCvsNoTag_KIN28med13D_15minNAPP1_20160331_rep1-2__S.cerevisiaeApr11_217747.bed;

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 med13Δ::KanMX4


title: yFR2184vsFR763-kin28as_15minNAPP1_Med6-18MYCvsNoTag_20151012_rep4

description:
Biological replicate 2 of 2. Med6 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes. Med6 ChIP sample (Cy5-labeled) was performed from a 18xMYC-tagged Med6 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED6 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med6 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR2184vsFR763-kin28as_15minNAPP1_Med6-18MYCvsNoTag_20151012_rep3-4__S.cerevisiaeApr11_366314.bed;

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 kin28::kin28as (L83G) [pSH579, CEN URA3 kin28as (L83G)] MED6-18xMYC::klTRP1


description:
Biological replicate 1 of 2. Med8 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes. Med8 ChIP sample (Cy5-labeled) was performed from a 9xMYC-tagged Med8 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED8 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med8 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR2183vsFR763-kin28as_15minNAPP1_Med8-9MYCvsNoTag_20151012_rep3-4__S.cerevisiaeApr11_975386.bed;

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 kin28::kin28as (L83G) [pSH579, CEN URA3 kin28as (L83G)] MED8-9xMYC::klTRP1


title: yFR1685vs762_Srb10-Cdk8-9MYC_WT_MYCvsNotag_20160406_rep2

description:
Biological replicate 2 of 2. Cdk8 occupancy in WT cells. Cdk8 ChIP sample (Cy5-labeled) was performed from a 9xMYC-tagged Cdk8 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the CDK8 gene is not tagged (Cy3-labeled).
genome browser-ready file for Cdk8 occupancy in WT cells
processed data file: SmthBED_yFR1685vs762_Srb10-Cdk8-9MYC_WT_MYCvsNotag_20160406_rep1-2__S.cerevisiaeApr11_12746.bed;

value: 9E10 (lab stock prepared by Pierre, 2015)


title: yFR1901vsFR763-kin28as_15minNAPP1_Srb6-13MYCvsNoTag_20151012_rep3

description:
Biological replicate 2 of 2. Med22 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes. Med22 ChIP sample (Cy5-labeled) was performed from a 13xMYC-tagged Med22 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED22 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med22 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1901vsFR763-kin28as_15minNAPP1_Srb6-Med22-13MYCvsUntagged_20151012_rep2-3__S.cerevisiaeApr11_34039.bed;

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 kin28::kin28as (L83G) [pSH579, CEN URA3 kin28as (L83G)] MED22-13xMYC::HPHr


description:
Biological replicate 1 of 2. Med13 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes. Med13 ChIP sample (Cy5-labeled) was performed from a 3xFLAG-tagged Med13 strain using the anti-FLAG antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED13 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med13 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1912vsFR763-kin28as_15minNAPP1_Srb9-Med13-FLAGvsUntagged_rep1-2__S.cerevisiaeApr11_46763

treatment_protocol:
6microM NA-PP1 for 15 min
IP protocol: Flag_M2_SigmaF3165. Anti-mouse Dynabeads (PanMouse) (50uL / ChIP) are washed twice with PBS + 0.5% BSA (freshly made), and resuspended in PBS + 0.5% BSA. Anti-Flag M2 (Sigma F3165) is added (5ug/ChIP), and incubated overnight at 4'C in haematological mixer. Beads are washed twice (with PBS/BSA or Lysis buffer), and resuspended in 30uL (PBS/BSA or Lysis buffer) / ChIP. 30uL of resuspended beads/antibody is added to 500 uL of lysate, and incubated overnight at 4'C in haematological mixer. Beads are then washed in cold room (using the Dynal magnet system) twice with 1mL ice cold Lysis Buffer (no protease inhibitors), twice with 1mL ice cold Lysis Buffer (no protease inhibitors) + additional 360mM NaCl, twice with ice cold Wash Buffer, and once with ice cold TE. Remaining liquid is removed by pipetting after a brief centrifugation at 4'C, and 50uL TE + 1% SDS is added for elution. Beads are incubated overnight at 65'C for elution and reverse crosslink. Note for Whole Cell Extracts (WCE): WCE are included in the protocol only at the crosslink reversal step. Add 50uL TE + 1% SDS to 5uL WCE, and put overnight at 65'C to reverse the crosslinks with the ChIP samples. Lysis Buffer (no protease inhibitors): 50mM HEPES-KOH, pH 7.5 140mM NaCl 1mM EDTA 1% Triton X-100 0.1% Na-deoxycholate Wash Buffer: 10mM Tris-HCl, pH 8.0 250mM LiCl 0.5% NP40 0.5% Na-deoxycholate 1mM EDTA.

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 kin28::kin28as (L83G) [pSH579, CEN URA3 kin28as (L83G)] MED13-3xFLAG::KanMX

value: Flag M2 R (Sigma)


description:
Biological replicate 1 of 2. Med8 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes. Med8 ChIP sample (Cy5-labeled) was performed from a 9xMYC-tagged Med8 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED8 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med8 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR2186vsFR762-WT_15minNAPP1_Med8-9MYCvsNoTag_20151012_rep3-4__S.cerevisiaeApr11_150444.bed;

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 MED8-9xMYC::klTRP1


title: yFR1898vsFR762-WT_15minNAPP1_Srb9-Med13-FLAGvsUntagged_rep2

description:
Biological replicate 2 of 2. Med13 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes. Med13 ChIP sample (Cy5-labeled) was performed from a 3xFLAG-tagged Med13 strain using the anti-FLAG antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED13 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med13 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1898vsFR762-WT_15minNAPP1_Srb9-Med13-FLAGvsUntagged_rep1-2__S.cerevisiaeApr11_201214

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 MED13-3xFLAG::KanMX


description:
Biological replicate 1 of 2. Med16 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes. Med16 ChIP sample (Cy5-labeled) was performed from a 18xMYC-tagged Med16 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED16 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med16 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR2185vsFR763-kin28as_15minNAPP1_Med16-Sin4-18MYCvsNoTag_20151012_rep3-4__S.cerevisiaeApr11_943117.bed;

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 kin28::kin28as (L83G) [pSH579, CEN URA3 kin28as (L83G)] MED16-18xMYC::klTRP1


title: yFR2185vsFR763-kin28as_15minNAPP1_Med16-Sin4-18MYCvsNoTag_20151012_rep4

description:
Biological replicate 2 of 2. Med16 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes. Med16 ChIP sample (Cy5-labeled) was performed from a 18xMYC-tagged Med16 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED16 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med16 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR2185vsFR763-kin28as_15minNAPP1_Med16-Sin4-18MYCvsNoTag_20151012_rep3-4__S.cerevisiaeApr11_943117.bed;

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 kin28::kin28as (L83G) [pSH579, CEN URA3 kin28as (L83G)]


title: yFR1912vsFR763-kin28as_15minNAPP1_Srb9-Med13-FLAGvsUntagged_rep2

description:
Biological replicate 2 of 2. Med13 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes. Med13 ChIP sample (Cy5-labeled) was performed from a 3xFLAG-tagged Med13 strain using the anti-FLAG antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED13 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med13 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1912vsFR763-kin28as_15minNAPP1_Srb9-Med13-FLAGvsUntagged_rep1-2__S.cerevisiaeApr11_46763


title: yFR2183vsFR763-kin28as_15minNAPP1_Med8-9MYCvsNoTag_20151012_rep4

description:
Biological replicate 2 of 2. Med8 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes. Med8 ChIP sample (Cy5-labeled) was performed from a 9xMYC-tagged Med8 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED8 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med8 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR2183vsFR763-kin28as_15minNAPP1_Med8-9MYCvsNoTag_20151012_rep3-4__S.cerevisiaeApr11_975386.bed;


description:
Biological replicate 1 of 2. Med13 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes. Med13 ChIP sample (Cy5-labeled) was performed from a 3xFLAG-tagged Med13 strain using the anti-FLAG antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED13 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med13 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1898vsFR762-WT_15minNAPP1_Srb9-Med13-FLAGvsUntagged_rep1-2__S.cerevisiaeApr11_201214


description:
Biological replicate 1 of 2. Med16 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes. Med16 ChIP sample (Cy5-labeled) was performed from a 18xMYC-tagged Med16 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED16 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med16 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR2188vsFR762-WT_15minNAPP1_Med16-Sin4-18MYCvsNoTag_20151012_rep3-4__S.cerevisiaeApr11_871273.bed;


title: yFR2186vsFR762-WT_15minNAPP1_Med8-9MYCvsNoTag_20151012_rep4

description:
Biological replicate 2 of 2. Med8 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes. Med8 ChIP sample (Cy5-labeled) was performed from a 9xMYC-tagged Med8 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED8 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med8 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR2186vsFR762-WT_15minNAPP1_Med8-9MYCvsNoTag_20151012_rep3-4__S.cerevisiaeApr11_150444.bed;


description:
Biological replicate 1 of 2. Med6 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes. Med6 ChIP sample (Cy5-labeled) was performed from a 18xMYC-tagged Med6 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED6 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med6 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR2184vsFR763-kin28as_15minNAPP1_Med6-18MYCvsNoTag_20151012_rep3-4__S.cerevisiaeApr11_366314.bed;


description:
Biological replicate 1 of 2. Med15 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes. Med15 ChIP sample (Cy5-labeled) was performed from a 9xMYC-tagged Med15 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED15 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med15 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1638vsFR763_Gal11-9MYCvsNoTag_kin28as_15minNAPP1_20160331_rep1-2__S.cerevisiaeApr11_614092.bed;

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 kin28::kin28as (L83G) MED15-9xMYC::klTRP1 [pSH579, CEN URA3 kin28as (L83G)]


title: yFR1938vsFR763-kin28as_15minNAPP1_Srb11-CycC-3FlagvsUntagged_20150316_rep2

description:
Biological replicate 2 of 2. CycC occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes. CycC ChIP sample (Cy5-labeled) was performed from a 3xFLAG-tagged CycC strain using the anti-FLAG antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the CYCC gene is not tagged (Cy3-labeled).
genome browser-ready file for CycC occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1938vsFR763-kin28as_15minNAPP1_Srb11-CycC-3FlagvsUntagged_20150316_rep1-2__S.cerevisiaeApr11_553303

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 kin28::kin28as (L83G) [pSH579, CEN URA3 kin28as (L83G)] CYCC-3xFLAG::kanMX


description:
Biological replicate 1 of 2. Cdk8 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes. Cdk8 ChIP sample (Cy5-labeled) was performed from a 3xFLAG-tagged Cdk8 strain using the anti-FLAG antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the CDK8 gene is not tagged (Cy3-labeled).
genome browser-ready file for Cdk8 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1937vsFR763-kin28as_15minNAPP1_Srb10-Cdk8-3FlagvsUntagged_20150316_rep1-2__S.cerevisiaeApr11_627601

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 kin28::kin28as (L83G) [pSH579, CEN URA3 kin28as (L83G)] CDK8-3xFLAG::kanMX


description:
Biological replicate 1 of 2. CycC occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes. CycC ChIP sample (Cy5-labeled) was performed from a 3xFLAG-tagged CycC strain using the anti-FLAG antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the CYCC gene is not tagged (Cy3-labeled).
genome browser-ready file for CycC occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1938vsFR763-kin28as_15minNAPP1_Srb11-CycC-3FlagvsUntagged_20150316_rep1-2__S.cerevisiaeApr11_553303


title: yFR1937vsFR763-kin28as_15minNAPP1_Srb10-Cdk8-3FlagvsUntagged_20150316_rep2

description:
Biological replicate 2 of 2. Cdk8 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes. Cdk8 ChIP sample (Cy5-labeled) was performed from a 3xFLAG-tagged Cdk8 strain using the anti-FLAG antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the CDK8 gene is not tagged (Cy3-labeled).
genome browser-ready file for Cdk8 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1937vsFR763-kin28as_15minNAPP1_Srb10-Cdk8-3FlagvsUntagged_20150316_rep1-2__S.cerevisiaeApr11_627601


title: yFR1905vsFR762-WT_15minNAPP1_Srb10-Cdk8-3FlagvsUntagged_20150316_rep2

description:
Biological replicate 2 of 2. Cdk8 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes. Cdk8 ChIP sample (Cy5-labeled) was performed from a 3xFLAG-tagged Cdk8 strain using the anti-FLAG antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the CDK8 gene is not tagged (Cy3-labeled).
genome browser-ready file for Cdk8 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1905vsFR762-WT_15minNAPP1_Srb10-Cdk8-3FlagvsUntagged_20150316_rep1-2__S.cerevisiaeApr11_139867

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 CDK8-3xFLAG::KanMX


description:
Biological replicate 1 of 2. Cdk8 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes. Cdk8 ChIP sample (Cy5-labeled) was performed from a 3xFLAG-tagged Cdk8 strain using the anti-FLAG antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the CDK8 gene is not tagged (Cy3-labeled).
genome browser-ready file for Cdk8 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1905vsFR762-WT_15minNAPP1_Srb10-Cdk8-3FlagvsUntagged_20150316_rep1-2__S.cerevisiaeApr11_139867

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0


description:
Biological replicate 1 of 2. Med17 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes. Med17 ChIP sample (Cy5-labeled) was performed from a 3xHA-tagged Med17 strain using the anti-HA antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED17 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med17 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1908vsFR762-WT_15minNAPP1_Srb4-Med17-3HAvsUntagged_rep1-2__S.cerevisiaeApr11_647158.bed;

treatment_protocol:
6microM NA-PP1 for 15 min
IP protocol: ChIP_HA-tag. Anti-mouse Dynabeads (50uL / ChIP) are washed twice with 10mL PBS + 0.5% BSA (freshly made), and resuspended in 1mL PBS + 0.5% BSA. F-7 anti-HA antibody (Santa Cruz)is added (15uL / ChIP), and incubated overnight at 4'C in haematological mixer. Beads are washed twice with 10mL PBS + 0.5% BSA (freshly made), and resuspended in 30uL PBS + 0.5% BSA / ChIP. A mix of 250uL of cell lysate + 250uL of Lysis Buffer containing protease inhibitors are added to 30uL beads, and incubated overnight at 4'C in haematological mixer. Beads are then washed (using the Dynal magnet system) twice with 1mL ice cold Lysis Buffer (no protease inhibitors), twice with 1mL ice cold Lysis Buffer (no protease inhibitors) + additional 360mM NaCl (720uL of 5M NaCl per 10mL lysis buffer), twice with ice cold Wash Buffer, and once with ice cold TE. Remaining liquid is removed by pipetting, and 50uL TE + 1% SDS is added. Beads are incubated overnight at 65'C to reverse the crosslinks. Lysis buffer: 50mM HEPES-KOH, pH 7.5 140mM NaCl 1mM EDTA 1% Triton X-100 0.1% Na-deoxycholate 1mM PMSF 1mM Benzamidine 10ug/mL Aprotinin 1ug/mL Leupeptin 1ug/mL. Pepstatin.

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 MED17-3xHA::HIS3

value: F7 (anti-HA)


title: yFR1908vsFR762-WT_15minNAPP1_Srb4-Med17-3HAvsUntagged_rep2

description:
Biological replicate 2 of 2. Med17 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes. Med17 ChIP sample (Cy5-labeled) was performed from a 3xHA-tagged Med17 strain using the anti-HA antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED17 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med17 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1908vsFR762-WT_15minNAPP1_Srb4-Med17-3HAvsUntagged_rep1-2__S.cerevisiaeApr11_647158.bed;


description:
Biological replicate 1 of 2. CycC occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes. CycC ChIP sample (Cy5-labeled) was performed from a 3xFLAG-tagged CycC strain using the anti-FLAG antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the CYCC gene is not tagged (Cy3-labeled).
genome browser-ready file for CycC occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1904vsFR762-WT_15minNAPP1_Srb11-CycC-3FlagvsUntagged_20150316_rep1-2__S.cerevisiaeApr11_715610

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 CYCC-3xFLAG::KanMX


title: yFR1903vsFR763-kin28as_15minNAPP1_Rgr1-Med14-13MYCvsUntagged_rep2

description:
Biological replicate 2 of 2. Med14 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes. Med14 ChIP sample (Cy5-labeled) was performed from a 13xMYC-tagged Med14 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED14 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med14 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1903vsFR763-kin28as_15minNAPP1_Rgr1-Med14-13MYCvsUntagged_rep1-2__S.cerevisiaeApr11_824063.bed;

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 kin28::kin28as (L83G) [pSH579, CEN URA3 kin28as (L83G)] MED14-13xMYC::HPHr

value: 9E10 (lab stock by AF)


description:
Biological replicate 1 of 2. Med17 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes. Med17 ChIP sample (Cy5-labeled) was performed from a 3xHA-tagged Med17 strain using the anti-HA antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED17 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med17 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1916vsFR763-kin28as_15minNAPP1_Srb4-Med17-3HAvsUntagged_rep1-2__S.cerevisiaeApr11_237668.bed;

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 kin28::kin28as (L83G) [pSH579, CEN URA3 kin28as (L83G)] MED17-3xHA::HIS3


title: yFR1916vsFR763-kin28as_15minNAPP1_Srb4-Med17-3HAvsUntagged_rep2

description:
Biological replicate 2 of 2. Med17 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes. Med17 ChIP sample (Cy5-labeled) was performed from a 3xHA-tagged Med17 strain using the anti-HA antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED17 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med17 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1916vsFR763-kin28as_15minNAPP1_Srb4-Med17-3HAvsUntagged_rep1-2__S.cerevisiaeApr11_237668.bed;


description:
Biological replicate 1 of 2. Med7 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes. Med7 ChIP sample (Cy5-labeled) was performed from a 13xMYC-tagged Med7 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED7 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med7 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1902vsFR763-kin28as_15minNAPP1_Med7-13MYCvsUntagged_rep1-2__S.cerevisiaeApr11_398296.bed;

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 kin28::kin28as (L83G) [pSH579, CEN URA3 kin28as (L83G)] MED7-13xMYC::HPHr


title: SUBJ.1560, SLE, week52

type: RNA

city: Indianapolis

first: Robert

middle: W

last: Hoffman

state: IN

address: Lilly Corporate Center

department: Lilly Bio-Medicines/Immunology

institute: Eli Lilly and Company

description: Gene expression measured in blood sample from SLE subject at week52 with LY 120 mg Q4W treatment

hyb_protocol: The cDNA generated during the amplification step was used in a fragmentation and labeling reaction to generate target for hybridization to the Affymetrix HTA2.0 arrays

scan_protocol: GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.

data_processing: HTA2.0 microarray pre-processing was performed. Probe level microarray data were summarized to two different levels, "gene" level and "exon-group" level. In both cases, the first two steps of the pre-processing were standard background correction and quantile normalization, as done in standard robust multi-array average (22). For the "gene" level analyses, data were then summarized by the sample effect estimated by robust regression (rlm function in the R MASS package) from a two-way ANOVA model with probe and sample effects. For the "exon-group" analysis (presented in the corresponding data matrix table), data were first summarized to the probeset level (as defined by Affymetrix) and low-expression probesets were filtered out as follows. An expression score was constructed for each probeset by computing the 80th percentile across all samples; probesets whose expression score was below the 95th percentile of the intronic normalization control probesets (normgene->intron) were discarded. The filtered probesets within a "transcript cluster" were then grouped using hierarchical clustering to form exon groups using the R hclust function. The distance metric between two probesets was defined as one minus the correlation of the pair. Probe level data were then summarized for each "exon-group" (cluster) using robust estimates of the sample effects as done in the "gene" level summarization.

label: biotin

molecule: total RNA

source_name: Blood sample from SLE subject at week52 with LY 120 mg Q4W treatment

label_protocol: Target preparation was performed according to Affymetrix WT Plus amplification protocol (www.affymetrix.com).

extract_protocol: Total RNA was extracted from whole blood (preserved in Tempus tubes) using the PerfectPureTM RNA Blood Kit as recommended by the manufacturer (Five Prime, South San Francisco, CA).

treatment_protocol: A subset of SLE patients were treated with the BAFF/BLyS-blocking, fully-human IgG4 monoclonal antibody, tabalumab (120 mg Q2W or 120 mg Q4W) for 16 weeks or 52 weeks prior to RNA extraction.

tag: time

tag: group

value: Systemic lupus erythematosus (SLE)

tag: treatment (q2w=once in 2 weeks; q4w=once in 4 weeks)

value: LY 120 mg Q4W

tag: batch

tag: race

value: white

tag: Sex

value: Female

tag: region

tag: adna_at_baseline (anti-dsdna level

value: Negative < 30 IU; Positive  ≥ 30 IU):Negative

tag: antidsdna_at_baseline (iu)

tag: c3base (low < 0.9 g/l; normal >= 0.9 g/l; h = high)

value: L

tag: c3_at_baseline (g/l)

tag: c4base (low < 0.1 g/l; normal >= 0.1 g/l; h = high)

tag: c4_at_baseline (g/l)

tag: tissue

value: Blood


title: SUBJ.1560, SLE, week16

description: Gene expression measured in blood sample from SLE subject at week16 with LY 120 mg Q4W treatment

source_name: Blood sample from SLE subject at week16 with LY 120 mg Q4W treatment


title: SUBJ.0638, SLE, week52

value: N


title: SUBJ.1595, SLE, week52

description: Gene expression measured in blood sample from SLE subject at week52 with LY 120 mg Q2W treatment

source_name: Blood sample from SLE subject at week52 with LY 120 mg Q2W treatment

value: LY 120 mg Q2W

value: europe

value: Negative < 30 IU; Positive  ≥ 30 IU):Positive


title: yFR1902vsFR763-kin28as_15minNAPP1_Med7-13MYCvsUntagged_rep2

description:
Biological replicate 2 of 2. Med7 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes. Med7 ChIP sample (Cy5-labeled) was performed from a 13xMYC-tagged Med7 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED7 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med7 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1902vsFR763-kin28as_15minNAPP1_Med7-13MYCvsUntagged_rep1-2__S.cerevisiaeApr11_398296.bed;

tag: strain

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 kin28::kin28as (L83G) [pSH579, CEN URA3 kin28as (L83G)]


description:
Biological replicate 1 of 2. Med14 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes. Med14 ChIP sample (Cy5-labeled) was performed from a 13xMYC-tagged Med14 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED14 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med14 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1903vsFR763-kin28as_15minNAPP1_Rgr1-Med14-13MYCvsUntagged_rep1-2__S.cerevisiaeApr11_824063.bed;

tag: strain information


title: SUBJ.1560, SLE, baseline

description: Gene expression measured in blood sample from SLE subject at baseline

source_name: Blood sample from SLE subject at baseline

value: baseline


title: yFR1901vsFR763-kin28as_15minNAPP1_Srb6-Med22-13MYCvsUntagged_rep2

description:
Biological replicate 1 of 2. Med22 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes. Med22 ChIP sample (Cy5-labeled) was performed from a 13xMYC-tagged Med22 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED22 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med22 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1901vsFR763-kin28as_15minNAPP1_Srb6-Med22-13MYCvsUntagged_20151012_rep2-3__S.cerevisiaeApr11_34039.bed;

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 kin28::kin28as (L83G) [pSH579, CEN URA3 kin28as (L83G)] MED22-13xMYC::HPHr

tag: antibody


title: SUBJ.0638, SLE, baseline

tag: time


title: yFR1896vsFR762-WT_15minNAPP1_Rgr1-Med14-13MYCvsUntagged_rep2

description:
Biological replicate 2 of 2. Med14 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes. Med14 ChIP sample (Cy5-labeled) was performed from a 13xMYC-tagged Med14 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED14 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med14 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1896vsFR762-WT_15minNAPP1_Rgr1-Med14-13MYCvsUntagged_rep1-2__S.cerevisiaeApr11_29443.bed;

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 MED14-13xMYC::HPHr

value: 9E10 (lab stock by AF)


title: SUBJ.0638, SLE, week16

tag: group


title: SUBJ.1595, SLE, week16

description: Gene expression measured in blood sample from SLE subject at week16 with LY 120 mg Q2W treatment

source_name: Blood sample from SLE subject at week16 with LY 120 mg Q2W treatment

tag: treatment (q2w=once in 2 weeks; q4w=once in 4 weeks)


title: yFR1897vsFR762-WT_15minNAPP1_Srb6-Med22-13MYCvsUntagged_rep2

description:
Biological replicate 2 of 2. Med22 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes. Med22 ChIP sample (Cy5-labeled) was performed from a 13xMYC-tagged Med22 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED22 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med22 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1897vsFR762-WT_15minNAPP1_Srb6-Med22-13MYCvsUntagged_rep1-2__S.cerevisiaeApr11_643381.bed;

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 MED22-13xMYC::HPHr


title: yFR1904vsFR762-WT_15minNAPP1_Srb11-CycC-3FlagvsUntagged_20150316_rep2

description:
Biological replicate 2 of 2. CycC occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes. CycC ChIP sample (Cy5-labeled) was performed from a 3xFLAG-tagged CycC strain using the anti-FLAG antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the CYCC gene is not tagged (Cy3-labeled).
genome browser-ready file for CycC occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1904vsFR762-WT_15minNAPP1_Srb11-CycC-3FlagvsUntagged_20150316_rep1-2__S.cerevisiaeApr11_715610

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 CYCC-3xFLAG::KanMX

value: Flag M2 R (Sigma)


title: SUBJ.1595, SLE, baseline

value: Systemic lupus erythematosus (SLE)

tag: batch

value: white


description:
Biological replicate 1 of 2. Med14 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes. Med14 ChIP sample (Cy5-labeled) was performed from a 13xMYC-tagged Med14 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED14 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med14 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1896vsFR762-WT_15minNAPP1_Rgr1-Med14-13MYCvsUntagged_rep1-2__S.cerevisiaeApr11_29443.bed;

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0


title: yFR1889vsFR763-kin28as_15minNAPP1_Rox3-Med19-18MYCvsUntagged_rep2

description:
Biological replicate 2 of 2. Med19 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes. Med19 ChIP sample (Cy5-labeled) was performed from a 18xMYC-tagged Med19 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED19 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med19 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1889vsFR763-kin28as_15minNAPP1_Rox3-Med19-18MYCvsUntagged_rep1-2__S.cerevisiaeApr11_3919.bed;

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 kin28::kin28as (L83G) [pSH579, CEN URA3 kin28as (L83G)] MED19-18xMYC::klTRP1


title: yFR1900vsFR762-WT_15minNAPP1_Med7-13MYCvsUntagged_rep2

description:
Biological replicate 2 of 2. Med7 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes. Med7 ChIP sample (Cy5-labeled) was performed from a 13xMYC-tagged Med7 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED7 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med7 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1900vsFR762-WT_15minNAPP1_Med7-13MYCvsUntagged_rep1-2__S.cerevisiaeApr11_644870.bed;

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 MED7-13xMYC::HPHr


description:
Biological replicate 1 of 2. Med19 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes. Med19 ChIP sample (Cy5-labeled) was performed from a 18xMYC-tagged Med19 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED19 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med19 occupancy in kin28as cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1889vsFR763-kin28as_15minNAPP1_Rox3-Med19-18MYCvsUntagged_rep1-2__S.cerevisiaeApr11_3919.bed;


title: SUBJ.1354, SLE, week52

description: Gene expression measured in blood sample from SLE subject at week52 with Placebo treatment

source_name: Blood sample from SLE subject at week52 with Placebo treatment

value: Placebo

tag: race

value: Female

value: N

value: Blood


title: SUBJ.1354, SLE, week16

description: Gene expression measured in blood sample from SLE subject at week16 with Placebo treatment

source_name: Blood sample from SLE subject at week16 with Placebo treatment

tag: Sex

value: Negative < 30 IU; Positive  ≥ 30 IU):Positive


title: SUBJ.0819, SLE, baseline

value: baseline

value: LY 120 mg Q2W

tag: region

value: europe

value: L


title: yFR1888vsFR762-WT_15minNAPP1_Rox3-Med19-18MYCvsUntagged_rep2

description:
Biological replicate 2 of 2. Med19 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes. Med19 ChIP sample (Cy5-labeled) was performed from a 18xMYC-tagged Med19 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED19 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med19 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1888vsFR762-WT_15minNAPP1_Rox3-Med19-18MYCvsUntagged_rep1-2__S.cerevisiaeApr11_349724.bed;

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 MED19-18xMYC::klTRP1

value: 9E10 (lab stock by AF)


description:
Biological replicate 1 of 2. Med19 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes. Med19 ChIP sample (Cy5-labeled) was performed from a 18xMYC-tagged Med19 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED19 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med19 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1888vsFR762-WT_15minNAPP1_Rox3-Med19-18MYCvsUntagged_rep1-2__S.cerevisiaeApr11_349724.bed;


description:
Biological replicate 1 of 2. Med22 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes. Med22 ChIP sample (Cy5-labeled) was performed from a 13xMYC-tagged Med22 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED22 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med22 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1897vsFR762-WT_15minNAPP1_Srb6-Med22-13MYCvsUntagged_rep1-2__S.cerevisiaeApr11_643381.bed;

value: MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 MED22-13xMYC::HPHr


description:
Biological replicate 1 of 2. Med7 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes. Med7 ChIP sample (Cy5-labeled) was performed from a 13xMYC-tagged Med7 strain using the anti-MYC antibody and hybridized against a similar ChIP sample performed from an isogenic strain where the MED7 gene is not tagged (Cy3-labeled).
genome browser-ready file for Med7 occupancy in WT cells treated with 6 microM NAPP1 for 15 minutes
processed data file: SmthBED_yFR1900vsFR762-WT_15minNAPP1_Med7-13MYCvsUntagged_rep1-2__S.cerevisiaeApr11_644870.bed;


title: SUBJ.1242, SLE, week52

description: Gene expression measured in blood sample from SLE subject at week52 with LY 120 mg Q2W treatment

value: Systemic lupus erythematosus (SLE)

tag: adna_at_baseline (anti-dsdna level


title: SUBJ.1242, SLE, week16

value: white

tag: antidsdna_at_baseline (iu)


title: SUBJ.0819, SLE, week16

tag: c3base (low < 0.9 g/l; normal >= 0.9 g/l; h = high)


title: SUBJ.1354, SLE, baseline

description: Gene expression measured in blood sample from SLE subject at baseline

tag: c3_at_baseline (g/l)


title: SUBJ.0819, SLE, week52

tag: c4base (low < 0.1 g/l; normal >= 0.1 g/l; h = high)


title: SUBJ.1100, SLE, week52

value: Negative < 30 IU; Positive  ≥ 30 IU):Negative

tag: c4_at_baseline (g/l)


title: SUBJ.0759, SLE, week16

description: Gene expression measured in blood sample from SLE subject at week16 with LY 120 mg Q4W treatment

value: LY 120 mg Q4W

value: Male

tag: tissue


title: SUBJ.1600, SLE, week16


title: SUBJ.0759, SLE, week52

description: Gene expression measured in blood sample from SLE subject at week52 with LY 120 mg Q4W treatment


title: SUBJ.1695, SLE, baseline

value: Placebo


title: SUBJ.1695, SLE, week16

value: Female


title: SUBJ.1600, SLE, week52

value: N


title: SUBJ.1695, SLE, week52

value: Blood


title: SUBJ.0038, SLE, week52


title: SUBJ.1201, SLE, baseline


title: SUBJ.0038, SLE, week16

description: Gene expression measured in blood sample from SLE subject at week16 with LY 120 mg Q2W treatment


title: SUBJ.0759, SLE, baseline

value: Negative < 30 IU; Positive  ≥ 30 IU):Positive


title: SUBJ.1242, SLE, baseline

value: baseline


title: SUBJ.1100, SLE, week16


title: SUBJ.1100, SLE, baseline


title: SUBJ.1600, SLE, baseline


title: SUBJ.0215, SLE, week16

value: LY 120 mg Q2W


title: SUBJ.0038, SLE, baseline


title: SUBJ.0215, SLE, week52


title: SUBJ.1759, SLE, week52


title: SUBJ.1759, SLE, week16


title: SUBJ.0215, SLE, baseline


title: SUBJ.1735, SLE, week52

value: europe


title: SUBJ.1759, SLE, baseline


title: SUBJ.1534, SLE, week16


title: SUBJ.1735, SLE, week16

value: L


title: SUBJ.1534, SLE, week52


title: SUBJ.1735, SLE, baseline


title: SUBJ.1534, SLE, baseline


title: SUBJ.1653, SLE, week52


title: SUBJ.1670, SLE, week52


title: SUBJ.0369, SLE, week52


title: SUBJ.0369, SLE, week16


title: SUBJ.1670, SLE, baseline


title: SUBJ.0437, SLE, week16


title: SUBJ.1653, SLE, week16


title: SUBJ.0369, SLE, baseline


title: SUBJ.0109, SLE, week52


title: SUBJ.0050, SLE, week52


title: SUBJ.1670, SLE, week16


title: SUBJ.0109, SLE, baseline


title: SUBJ.0437, SLE, baseline


title: SUBJ.1653, SLE, baseline


title: SUBJ.0050, SLE, week16


title: SUBJ.0050, SLE, baseline


title: SUBJ.0437, SLE, week52


title: SUBJ.0109, SLE, week16


title: SUBJ.1405, SLE, week52


title: SUBJ.1166, SLE, week52


title: SUBJ.1405, SLE, baseline


title: SUBJ.0829, SLE, week52


title: SUBJ.1166, SLE, baseline


title: SUBJ.0877, SLE, week52


title: SUBJ.0829, SLE, baseline


title: SUBJ.0877, SLE, week16


title: SUBJ.0877, SLE, baseline


title: SUBJ.0325, SLE, week52


title: SUBJ.0325, SLE, week16


title: SUBJ.0865, SLE, week52

value: multiple


title: SUBJ.0325, SLE, baseline


title: SUBJ.0865, SLE, week16


title: SUBJ.1410, SLE, week52


title: SUBJ.0865, SLE, baseline


title: SUBJ.1410, SLE, baseline


title: SUBJ.0604, SLE, week52


title: SUBJ.0604, SLE, week16


title: SUBJ.0274, SLE, week52


title: SUBJ.0274, SLE, baseline


title: SUBJ.1166, SLE, week16


title: SUBJ.0289, SLE, week16


title: SUBJ.0951, SLE, week52


title: SUBJ.0274, SLE, week16


title: SUBJ.1675, SLE, baseline


title: SUBJ.0289, SLE, week52


title: SUBJ.0951, SLE, week16


title: SUBJ.0576, SLE, week52


title: SUBJ.0576, SLE, week16


title: SUBJ.0829, SLE, week16


title: SUBJ.0289, SLE, baseline


title: SUBJ.0951, SLE, baseline


title: SUBJ.0846, SLE, week16


title: SUBJ.1201, SLE, week52


title: SUBJ.0846, SLE, baseline


title: SUBJ.1570, SLE, week16


title: SUBJ.0846, SLE, week52


title: SUBJ.1323, SLE, week52


title: SUBJ.1323, SLE, week16


title: SUBJ.1570, SLE, week52


title: SUBJ.1675, SLE, week52


title: SUBJ.1570, SLE, baseline


title: SUBJ.1194, SLE, week52


title: SUBJ.1323, SLE, baseline


title: SUBJ.0433, SLE, week16


title: SUBJ.1194, SLE, baseline


title: SUBJ.0433, SLE, week52


title: SUBJ.0172, SLE, week52


title: SUBJ.0172, SLE, week16


title: SUBJ.1722, SLE, week16

value: asian


title: SUBJ.1722, SLE, week52


title: SUBJ.0172, SLE, baseline


title: SUBJ.1722, SLE, baseline


title: SUBJ.0217, SLE, week16


title: SUBJ.0997, SLE, week16


title: SUBJ.0997, SLE, baseline


title: SUBJ.0217, SLE, baseline


title: SUBJ.1582, SLE, week16


title: SUBJ.1582, SLE, week52


title: SUBJ.1319, SLE, week16


title: SUBJ.1319, SLE, week52


title: SUBJ.1319, SLE, baseline


title: SUBJ.0938, SLE, week52


title: SUBJ.0938, SLE, week16


title: SUBJ.1693, SLE, week52


title: SUBJ.0938, SLE, baseline


title: SUBJ.1693, SLE, week16


title: SUBJ.1582, SLE, baseline


title: SUBJ.0433, SLE, baseline


title: SUBJ.1194, SLE, week16


title: SUBJ.0490, SLE, week52


title: SUBJ.0490, SLE, week16


title: SUBJ.1649, SLE, week52


title: SUBJ.0997, SLE, week52


title: SUBJ.0490, SLE, baseline


title: SUBJ.0217, SLE, week52


title: SUBJ.0883, SLE, week16


title: SUBJ.0883, SLE, week52


title: SUBJ.1649, SLE, baseline


title: SUBJ.1566, SLE, week16


title: SUBJ.0883, SLE, baseline


title: SUBJ.1566, SLE, baseline


title: SUBJ.1566, SLE, week52


title: SUBJ.0840, SLE, week52


title: SUBJ.0300, SLE, baseline


title: SUBJ.0300, SLE, week16


title: SUBJ.1768, SLE, week52


title: SUBJ.1768, SLE, week16


title: SUBJ.1768, SLE, baseline


title: SUBJ.0631, SLE, week52


title: SUBJ.0062, SLE, week52


title: SUBJ.0631, SLE, week16


title: SUBJ.1693, SLE, baseline


title: SUBJ.0062, SLE, week16


title: SUBJ.1649, SLE, week16


title: SUBJ.0656, SLE, week52


title: SUBJ.0631, SLE, baseline


title: SUBJ.0062, SLE, baseline


title: SUBJ.1794, SLE, week16


title: SUBJ.1760, SLE, week16


title: SUBJ.1794, SLE, baseline


title: SUBJ.1760, SLE, baseline


title: SUBJ.0656, SLE, baseline


title: SUBJ.1794, SLE, week52


title: SUBJ.0300, SLE, week52


title: SUBJ.0859, SLE, week16


title: SUBJ.1760, SLE, week52


title: SUBJ.0892, SLE, week52


title: SUBJ.0892, SLE, baseline


title: SUBJ.0413, SLE, week52


title: SUBJ.0859, SLE, baseline


title: SUBJ.0824, SLE, week52


title: SUBJ.0824, SLE, week16


title: SUBJ.0413, SLE, baseline


title: SUBJ.0818, SLE, week16


title: SUBJ.0824, SLE, baseline


title: SUBJ.0674, SLE, week16


title: SUBJ.0228, SLE, week52


title: SUBJ.0413, SLE, week16


title: SUBJ.0656, SLE, week16


title: SUBJ.0228, SLE, week16


title: SUBJ.0535, SLE, week52


title: SUBJ.0601, SLE, week16


title: SUBJ.0859, SLE, week52


title: SUBJ.0674, SLE, baseline


title: SUBJ.0674, SLE, week52


title: SUBJ.0535, SLE, week16


title: SUBJ.1674, SLE, week52

value: H


title: SUBJ.0228, SLE, baseline

value: Systemic lupus erythematosus (SLE)


title: SUBJ.0535, SLE, baseline


title: SUBJ.0546, SLE, week16


title: SUBJ.0546, SLE, week52


title: SUBJ.1674, SLE, baseline


title: SUBJ.0546, SLE, baseline


title: SUBJ.1674, SLE, week16


title: SUBJ.0420, SLE, week52

value: white


title: SUBJ.0818, SLE, baseline


title: SUBJ.0698, SLE, week52


title: SUBJ.0698, SLE, week16


title: SUBJ.0420, SLE, baseline

value: Negative < 30 IU; Positive  ≥ 30 IU):Negative


title: SUBJ.0420, SLE, week16


title: SUBJ.1175, SLE, week52


title: SUBJ.1300, SLE, week52

value: LY 120 mg Q4W


title: SUBJ.0041, SLE, baseline


title: SUBJ.0698, SLE, baseline


title: SUBJ.1175, SLE, week16


title: SUBJ.1300, SLE, week16


title: SUBJ.1382, SLE, week16


title: SUBJ.1300, SLE, baseline


title: SUBJ.0349, SLE, week16


title: SUBJ.1382, SLE, week52


title: SUBJ.0349, SLE, week52


title: SUBJ.1382, SLE, baseline


title: SUBJ.0349, SLE, baseline


title: SUBJ.0441, SLE, baseline

value: Male


title: SUBJ.0441, SLE, week52


title: SUBJ.1049, SLE, week52


title: SUBJ.1049, SLE, week16


title: SUBJ.0658, SLE, week52


title: SUBJ.0658, SLE, week16


title: SUBJ.1049, SLE, baseline


title: SUBJ.0041, SLE, week52


title: SUBJ.1483, SLE, week52


title: SUBJ.1483, SLE, week16


title: SUBJ.1175, SLE, baseline


title: SUBJ.1250, SLE, week16


title: SUBJ.1483, SLE, baseline


title: SUBJ.1250, SLE, week52


title: SUBJ.0496, SLE, week16


title: SUBJ.0496, SLE, week52


title: SUBJ.1776, SLE, week52

value: Placebo


title: SUBJ.0496, SLE, baseline

value: Female


title: SUBJ.0441, SLE, week16


title: SUBJ.1776, SLE, baseline

value: N


title: SUBJ.0663, SLE, week16

value: Blood


title: SUBJ.1438, SLE, week16

value: Negative < 30 IU; Positive  ≥ 30 IU):Positive


title: SUBJ.1438, SLE, week52


title: SUBJ.0663, SLE, week52


title: SUBJ.0663, SLE, baseline

value: baseline


title: SUBJ.1438, SLE, baseline

value: LY 120 mg Q2W


title: SUBJ.0284, SLE, week52

value: europe


title: SUBJ.0284, SLE, week16

value: L


title: SUBJ.0440, SLE, week52


title: SUBJ.0284, SLE, baseline


title: SUBJ.0979, SLE, baseline


title: SUBJ.0440, SLE, baseline


title: SUBJ.0979, SLE, week52


title: SUBJ.0979, SLE, week16


title: SUBJ.1381, SLE, week52


title: SUBJ.1381, SLE, week16


title: SUBJ.1250, SLE, baseline


title: SUBJ.1441, SLE, week52


title: SUBJ.1441, SLE, week16


title: SUBJ.0695, SLE, week16


title: SUBJ.0695, SLE, baseline


title: SUBJ.1381, SLE, baseline


title: SUBJ.0695, SLE, week52


title: SUBJ.1441, SLE, baseline


title: SUBJ.0658, SLE, baseline


title: SUBJ.1776, SLE, week16


title: SUBJ.1652, SLE, week16


title: SUBJ.0756, SLE, week52


title: SUBJ.0313, SLE, baseline


title: SUBJ.1652, SLE, baseline


title: SUBJ.0266, SLE, week52


title: SUBJ.0756, SLE, baseline


title: SUBJ.0690, SLE, week52


title: SUBJ.0266, SLE, week16


title: SUBJ.0552, SLE, week52


title: SUBJ.0552, SLE, week16


title: SUBJ.0552, SLE, baseline


title: SUBJ.0313, SLE, week52


title: SUBJ.1652, SLE, week52


title: SUBJ.0690, SLE, baseline


title: SUBJ.0313, SLE, week16


title: SUBJ.0057, SLE, week52


title: SUBJ.0057, SLE, week16


title: SUBJ.0057, SLE, baseline


title: SUBJ.1399, SLE, week16


title: SUBJ.0756, SLE, week16


title: SUBJ.0225, SLE, week16


title: SUBJ.1436, SLE, week52


title: SUBJ.1436, SLE, baseline


title: SUBJ.0225, SLE, baseline


title: SUBJ.1399, SLE, week52


title: SUBJ.0393, SLE, week52


title: SUBJ.0393, SLE, week16


title: SUBJ.0266, SLE, baseline


title: SUBJ.1436, SLE, week16


title: SUBJ.0213, SLE, week52


title: SUBJ.0213, SLE, week16


title: SUBJ.1285, SLE, week52


title: SUBJ.0225, SLE, week52


title: SUBJ.1285, SLE, week16


title: SUBJ.0213, SLE, baseline


title: SUBJ.1285, SLE, baseline


title: SUBJ.1621, SLE, week52


title: SUBJ.0562, SLE, week52


title: SUBJ.0380, SLE, week52


title: SUBJ.1621, SLE, baseline


title: SUBJ.1621, SLE, week16


title: SUBJ.0380, SLE, baseline


title: SUBJ.0562, SLE, baseline


title: SUBJ.1399, SLE, baseline


title: SUBJ.0380, SLE, week16


title: SUBJ.1641, SLE, week52


title: SUBJ.1167, SLE, week52


title: SUBJ.1167, SLE, baseline


title: SUBJ.1167, SLE, week16


title: SUBJ.1641, SLE, week16


title: SUBJ.0107, SLE, week16


title: SUBJ.0107, SLE, week52


title: SUBJ.1048, SLE, week16


title: SUBJ.1048, SLE, week52


title: SUBJ.0670, SLE, week52


title: SUBJ.1048, SLE, baseline


title: SUBJ.0107, SLE, baseline


title: SUBJ.0670, SLE, week16


title: SUBJ.1753, SLE, week52


title: SUBJ.0204, SLE, week52


title: SUBJ.0204, SLE, week16


title: SUBJ.1753, SLE, baseline


title: SUBJ.0204, SLE, baseline


title: SUBJ.1753, SLE, week16


title: SUBJ.0670, SLE, baseline


title: SUBJ.0089, SLE, week16


title: SUBJ.1641, SLE, baseline


title: SUBJ.0941, SLE, week16


title: SUBJ.0089, SLE, baseline


title: SUBJ.1428, SLE, week16


title: SUBJ.1428, SLE, week52


title: SUBJ.0941, SLE, week52


title: SUBJ.0941, SLE, baseline


title: SUBJ.1428, SLE, baseline


title: SUBJ.0245, SLE, week52


title: SUBJ.1042, SLE, week16


title: SUBJ.0555, SLE, week52


title: SUBJ.1042, SLE, baseline


title: SUBJ.0555, SLE, week16


title: SUBJ.0555, SLE, baseline


title: SUBJ.0245, SLE, baseline


title: SUBJ.1123, SLE, week52


title: SUBJ.0089, SLE, week52


title: SUBJ.1383, SLE, week52


title: SUBJ.0332, SLE, week52


title: SUBJ.0332, SLE, week16


title: SUBJ.1123, SLE, week16


title: SUBJ.0520, SLE, week16


title: SUBJ.1123, SLE, baseline


title: SUBJ.0332, SLE, baseline


title: SUBJ.1383, SLE, baseline


title: SUBJ.1706, SLE, week52


title: SUBJ.1706, SLE, week16


title: SUBJ.1520, SLE, week52


title: SUBJ.1706, SLE, baseline


title: SUBJ.0774, SLE, week52


title: SUBJ.1520, SLE, week16


title: SUBJ.0774, SLE, baseline


title: SUBJ.1298, SLE, week16


title: SUBJ.1520, SLE, baseline


title: SUBJ.1042, SLE, week52


title: SUBJ.1228, SLE, week52

value: multiple


title: SUBJ.0774, SLE, week16


title: SUBJ.1228, SLE, week16


title: SUBJ.1298, SLE, week52


title: SUBJ.0520, SLE, baseline


title: SUBJ.1228, SLE, baseline


title: SUBJ.1479, SLE, week52


title: SUBJ.0813, SLE, week52


title: SUBJ.1479, SLE, baseline


title: SUBJ.1479, SLE, week16


title: SUBJ.0813, SLE, week16


title: SUBJ.1292, SLE, week16


title: SUBJ.0813, SLE, baseline


title: SUBJ.1540, SLE, week52


title: SUBJ.1195, SLE, week52


title: SUBJ.1540, SLE, week16


title: SUBJ.1292, SLE, baseline


title: SUBJ.1195, SLE, week16


title: SUBJ.1292, SLE, week52


title: SUBJ.1195, SLE, baseline


title: SUBJ.1273, SLE, week52


title: SUBJ.1273, SLE, week16


title: SUBJ.1273, SLE, baseline


title: SUBJ.0382, SLE, baseline


title: SUBJ.1812, SLE, week16


title: SUBJ.0382, SLE, week52


title: SUBJ.1812, SLE, baseline


title: SUBJ.1812, SLE, week52


title: SUBJ.1320, SLE, week52


title: SUBJ.0382, SLE, week16


title: SUBJ.0453, SLE, week52


title: SUBJ.1320, SLE, week16


title: SUBJ.0453, SLE, baseline


title: SUBJ.1320, SLE, baseline


title: SUBJ.1486, SLE, week52


title: SUBJ.1486, SLE, week16


title: SUBJ.1564, SLE, week16


title: SUBJ.1564, SLE, baseline


title: SUBJ.0453, SLE, week16


title: SUBJ.0984, SLE, week52


title: SUBJ.0112, SLE, week52


title: SUBJ.0984, SLE, baseline


title: SUBJ.0984, SLE, week16


title: SUBJ.0775, SLE, week16


title: SUBJ.0998, SLE, week52


title: SUBJ.0112, SLE, baseline


title: SUBJ.0775, SLE, baseline


title: SUBJ.1645, SLE, week52


title: SUBJ.0998, SLE, week16


title: SUBJ.0998, SLE, baseline


title: SUBJ.0322, SLE, week52


title: SUBJ.1645, SLE, week16


title: SUBJ.0322, SLE, week16


title: SUBJ.1510, SLE, week52


title: SUBJ.1564, SLE, week52


title: SUBJ.1011, SLE, week52


title: SUBJ.0322, SLE, baseline


title: SUBJ.1510, SLE, week16


title: SUBJ.1011, SLE, week16


title: SUBJ.1645, SLE, baseline


title: SUBJ.1509, SLE, week16


title: SUBJ.1510, SLE, baseline


title: SUBJ.0775, SLE, week52


title: SUBJ.0112, SLE, week16


title: SUBJ.1011, SLE, baseline


title: SUBJ.1824, SLE, week52


title: SUBJ.1509, SLE, week52


title: SUBJ.1824, SLE, week16


title: SUBJ.1509, SLE, baseline


title: SUBJ.1779, SLE, week52


title: SUBJ.1824, SLE, baseline


title: SUBJ.0726, SLE, week16


title: SUBJ.1779, SLE, baseline


title: SUBJ.0147, SLE, week52


title: SUBJ.1779, SLE, week16


title: SUBJ.0726, SLE, week52


title: SUBJ.0047, SLE, week52


title: SUBJ.0147, SLE, baseline


title: SUBJ.0147, SLE, week16


title: SUBJ.0288, SLE, week16


title: SUBJ.0908, SLE, week52


title: SUBJ.0288, SLE, baseline


title: SUBJ.1368, SLE, week52


title: SUBJ.1180, SLE, week52


title: SUBJ.1368, SLE, week16


title: SUBJ.0908, SLE, baseline


title: SUBJ.1180, SLE, baseline


title: SUBJ.1180, SLE, week16


title: SUBJ.1368, SLE, baseline


title: SUBJ.0438, SLE, week52


title: SUBJ.0356, SLE, week52


title: SUBJ.0438, SLE, week16


title: SUBJ.0878, SLE, baseline


title: SUBJ.0438, SLE, baseline


title: SUBJ.0878, SLE, week16


title: SUBJ.0878, SLE, week52


title: SUBJ.0356, SLE, baseline


title: SUBJ.0726, SLE, baseline


title: SUBJ.0356, SLE, week16


title: SUBJ.0450, SLE, week52


title: SUBJ.0450, SLE, week16


title: SUBJ.0450, SLE, baseline


title: SUBJ.0047, SLE, week16


title: SUBJ.0523, SLE, week52


title: SUBJ.0047, SLE, baseline


title: SUBJ.0351, SLE, baseline


title: SUBJ.0908, SLE, week16


title: SUBJ.0523, SLE, week16


title: SUBJ.0523, SLE, baseline


title: SUBJ.1082, SLE, week16


title: SUBJ.1472, SLE, week16


title: SUBJ.1082, SLE, baseline


title: SUBJ.1168, SLE, week16


title: SUBJ.1168, SLE, week52


title: SUBJ.1472, SLE, week52


title: SUBJ.1472, SLE, baseline


title: SUBJ.1168, SLE, baseline


title: SUBJ.0351, SLE, week52


title: SUBJ.0929, SLE, week16


title: SUBJ.1422, SLE, baseline


title: SUBJ.1422, SLE, week52


title: SUBJ.0342, SLE, week16


title: SUBJ.0342, SLE, week52


title: SUBJ.0929, SLE, baseline


title: SUBJ.0351, SLE, week16


title: SUBJ.0929, SLE, week52


title: SUBJ.1422, SLE, week16


title: SUBJ.1416, SLE, week16


title: SUBJ.0342, SLE, baseline


title: SUBJ.0599, SLE, week52


title: SUBJ.1416, SLE, week52


title: SUBJ.0599, SLE, week16


title: SUBJ.0599, SLE, baseline


title: SUBJ.1082, SLE, week52


title: SUBJ.1554, SLE, week16


title: SUBJ.1554, SLE, baseline


title: SUBJ.0913, SLE, week52


title: SUBJ.0913, SLE, week16


title: SUBJ.1785, SLE, week52


title: SUBJ.0913, SLE, baseline


title: SUBJ.1785, SLE, week16


title: SUBJ.1010, SLE, week52


title: SUBJ.1010, SLE, week16


title: SUBJ.1785, SLE, baseline


title: SUBJ.1475, SLE, week52


title: SUBJ.1010, SLE, baseline


title: SUBJ.1475, SLE, week16


title: SUBJ.1416, SLE, baseline


title: SUBJ.1475, SLE, baseline


title: SUBJ.1341, SLE, week16


title: SUBJ.1341, SLE, baseline


title: SUBJ.0455, SLE, week52


title: SUBJ.0911, SLE, week52


title: SUBJ.1389, SLE, week52


title: SUBJ.0911, SLE, baseline


title: SUBJ.1239, SLE, week52


title: SUBJ.0911, SLE, week16


title: SUBJ.1239, SLE, week16


title: SUBJ.1389, SLE, baseline


title: SUBJ.1610, SLE, week52


title: SUBJ.1341, SLE, week52


title: SUBJ.1610, SLE, week16


title: SUBJ.1239, SLE, baseline


title: SUBJ.1389, SLE, week16


title: SUBJ.1554, SLE, week52


title: SUBJ.1610, SLE, baseline


title: SUBJ.0474, SLE, week16


title: SUBJ.1238, SLE, week52

value: asian


title: SUBJ.0474, SLE, baseline


title: SUBJ.0509, SLE, week52


title: SUBJ.0474, SLE, week52


title: SUBJ.0509, SLE, week16


title: SUBJ.0455, SLE, baseline


title: SUBJ.0465, SLE, week52


title: SUBJ.0509, SLE, baseline


title: SUBJ.0465, SLE, week16


title: SUBJ.0465, SLE, baseline


title: SUBJ.0231, SLE, week52


title: SUBJ.0377, SLE, week52


title: SUBJ.1742, SLE, week16


title: SUBJ.0166, SLE, week52


title: SUBJ.1742, SLE, week52


title: SUBJ.0231, SLE, baseline


title: SUBJ.0377, SLE, week16


title: SUBJ.0735, SLE, week52


title: SUBJ.0157, SLE, week16


title: SUBJ.0166, SLE, week16


title: SUBJ.0377, SLE, baseline


title: SUBJ.1238, SLE, week16


title: SUBJ.0157, SLE, week52


title: SUBJ.0060, SLE, week52


title: SUBJ.0060, SLE, week16


title: SUBJ.0735, SLE, baseline


title: SUBJ.0060, SLE, baseline


title: SUBJ.0493, SLE, week52


title: SUBJ.0231, SLE, week16


title: SUBJ.1238, SLE, baseline


title: SUBJ.0493, SLE, baseline


title: SUBJ.0735, SLE, week16


title: SUBJ.0493, SLE, week16


title: SUBJ.0166, SLE, baseline


title: SUBJ.0703, SLE, week52


title: SUBJ.0703, SLE, week16


title: SUBJ.0703, SLE, baseline


title: SUBJ.1742, SLE, baseline


title: SUBJ.0689, SLE, week52


title: SUBJ.0689, SLE, baseline


title: SUBJ.0272, SLE, week16


title: SUBJ.0272, SLE, week52


title: SUBJ.0272, SLE, baseline


title: SUBJ.1573, SLE, week16


title: SUBJ.1573, SLE, baseline


title: SUBJ.0273, SLE, week52


title: SUBJ.0273, SLE, week16


title: SUBJ.0273, SLE, baseline


title: SUBJ.0331, SLE, week52


title: SUBJ.0331, SLE, week16


title: SUBJ.0230, SLE, week52


title: SUBJ.0230, SLE, week16


title: SUBJ.0363, SLE, week52


title: SUBJ.0363, SLE, week16


title: SUBJ.1493, SLE, week52


title: SUBJ.0230, SLE, baseline


title: SUBJ.1493, SLE, week16


title: SUBJ.0363, SLE, baseline


title: SUBJ.1493, SLE, baseline


title: SUBJ.0689, SLE, week16


title: SUBJ.1122, SLE, baseline


title: SUBJ.1573, SLE, week52


title: SUBJ.0986, SLE, week16


title: SUBJ.0986, SLE, baseline


title: SUBJ.1710, SLE, week52

value: H


title: SUBJ.0986, SLE, week52

value: Systemic lupus erythematosus (SLE)


title: SUBJ.0331, SLE, baseline

value: white


title: SUBJ.1122, SLE, week52

value: Negative < 30 IU; Positive  ≥ 30 IU):Negative


title: SUBJ.1122, SLE, week16


title: SUBJ.1710, SLE, week16

value: LY 120 mg Q4W


title: SUBJ.0666, SLE, week52


title: SUBJ.0619, SLE, week52


title: SUBJ.0619, SLE, week16


title: SUBJ.0666, SLE, baseline


title: SUBJ.0619, SLE, baseline


title: SUBJ.1603, SLE, week16


title: SUBJ.1603, SLE, week52


title: SUBJ.1710, SLE, baseline


title: SUBJ.1603, SLE, baseline


title: SUBJ.0626, SLE, week16


title: SUBJ.1391, SLE, week52


title: SUBJ.1391, SLE, week16


title: SUBJ.0626, SLE, baseline


title: SUBJ.1449, SLE, week16


title: SUBJ.0930, SLE, week52


title: SUBJ.1449, SLE, week52


title: SUBJ.1369, SLE, week52


title: SUBJ.1784, SLE, week16


title: SUBJ.1784, SLE, week52


title: SUBJ.1391, SLE, baseline


title: SUBJ.0117, SLE, baseline


title: SUBJ.0930, SLE, baseline


title: SUBJ.0117, SLE, week16


title: SUBJ.0117, SLE, week52


title: SUBJ.1449, SLE, baseline


title: SUBJ.1369, SLE, baseline


title: SUBJ.0666, SLE, week16


title: SUBJ.0742, SLE, week52


title: SUBJ.0742, SLE, week16


title: SUBJ.1369, SLE, week16


title: SUBJ.0626, SLE, week52


title: SUBJ.0839, SLE, week52


title: SUBJ.0930, SLE, week16


title: SUBJ.1083, SLE, week52


title: SUBJ.0839, SLE, baseline


title: SUBJ.0742, SLE, baseline


title: SUBJ.0839, SLE, week16


title: SUBJ.1083, SLE, week16


title: SUBJ.0743, SLE, week52

value: Male


title: SUBJ.1083, SLE, baseline


title: SUBJ.1784, SLE, baseline


title: SUBJ.0743, SLE, week16


title: SUBJ.0743, SLE, baseline


title: SUBJ.1724, SLE, week52


title: SUBJ.1724, SLE, week16


title: SUBJ.1724, SLE, baseline


title: SUBJ.0198, SLE, week52

value: Placebo


title: SUBJ.0028, SLE, week16

value: Female


title: SUBJ.0028, SLE, week52

value: N


title: SUBJ.1006, SLE, week16

value: Blood


title: SUBJ.1006, SLE, week52


title: SUBJ.0028, SLE, baseline

value: Negative < 30 IU; Positive  ≥ 30 IU):Positive


title: SUBJ.0198, SLE, baseline

value: baseline


title: SUBJ.1006, SLE, baseline

value: LY 120 mg Q2W


title: SUBJ.0198, SLE, week16


title: SUBJ.0629, SLE, baseline


title: SUBJ.0629, SLE, week52


title: SUBJ.0629, SLE, week16


title: SUBJ.1602, SLE, week52

value: europe


title: SUBJ.1259, SLE, week16


title: SUBJ.1259, SLE, baseline


title: SUBJ.1602, SLE, week16


title: SUBJ.1271, SLE, baseline


title: SUBJ.1602, SLE, baseline


title: SUBJ.0259, SLE, week16

value: L


title: SUBJ.0607, SLE, week16


title: SUBJ.0607, SLE, week52


title: SUBJ.1271, SLE, week16


title: SUBJ.0607, SLE, baseline


title: SUBJ.0390, SLE, week16


title: SUBJ.1259, SLE, week52


title: SUBJ.1271, SLE, week52


title: SUBJ.0598, SLE, week52


title: SUBJ.1138, SLE, week52


title: SUBJ.1138, SLE, week16


title: SUBJ.1138, SLE, baseline


title: SUBJ.0598, SLE, week16


title: SUBJ.0390, SLE, baseline


title: SUBJ.0598, SLE, baseline


title: SUBJ.0948, SLE, week52


title: SUBJ.0948, SLE, baseline


title: SUBJ.1513, SLE, week52


title: SUBJ.1513, SLE, baseline


title: SUBJ.1513, SLE, week16


title: SUBJ.0482, SLE, week52


title: SUBJ.0482, SLE, baseline


title: SUBJ.0482, SLE, week16


title: SUBJ.0582, SLE, week16


title: SUBJ.1762, SLE, week52


title: SUBJ.0582, SLE, week52


title: SUBJ.1762, SLE, week16


title: SUBJ.0259, SLE, baseline


title: SUBJ.0390, SLE, week52


title: SUBJ.1762, SLE, baseline


title: SUBJ.1598, SLE, week16


title: SUBJ.1598, SLE, week52


title: SUBJ.1598, SLE, baseline


title: SUBJ.1274, SLE, week16


title: SUBJ.1274, SLE, week52


title: SUBJ.1278, SLE, week52


title: SUBJ.1274, SLE, baseline


title: SUBJ.1565, SLE, week52


title: SUBJ.1278, SLE, week16


title: SUBJ.1565, SLE, baseline


title: SUBJ.1278, SLE, baseline


title: SUBJ.1081, SLE, week52


title: SUBJ.1081, SLE, week16


title: SUBJ.1081, SLE, baseline


title: SUBJ.0942, SLE, week16


title: SUBJ.0233, SLE, week16


title: SUBJ.0233, SLE, week52


title: SUBJ.0942, SLE, week52


title: SUBJ.0233, SLE, baseline


title: SUBJ.1565, SLE, week16


title: SUBJ.1522, SLE, week16


title: SUBJ.1522, SLE, week52


title: SUBJ.0528, SLE, week52


title: SUBJ.0056, SLE, week16


title: SUBJ.1522, SLE, baseline


title: SUBJ.0528, SLE, baseline


title: SUBJ.0528, SLE, week16


title: SUBJ.0942, SLE, baseline


title: SUBJ.0056, SLE, week52


title: SUBJ.0660, SLE, week16


title: SUBJ.1543, SLE, week16


title: SUBJ.0660, SLE, week52


title: SUBJ.0056, SLE, baseline


title: SUBJ.1543, SLE, baseline


title: SUBJ.1543, SLE, week52


title: SUBJ.1806, SLE, week52


title: SUBJ.1806, SLE, week16


title: SUBJ.1222, SLE, week52


title: SUBJ.1806, SLE, baseline


title: SUBJ.1687, SLE, week52


title: SUBJ.1222, SLE, baseline


title: SUBJ.1687, SLE, week16


title: SUBJ.1687, SLE, baseline


title: SUBJ.0364, SLE, week52


title: SUBJ.1084, SLE, week52


title: SUBJ.0364, SLE, week16


title: SUBJ.0364, SLE, baseline


title: SUBJ.0660, SLE, baseline


title: SUBJ.0292, SLE, week16


title: SUBJ.0292, SLE, week52


title: SUBJ.1084, SLE, baseline


title: SUBJ.1294, SLE, week16


title: SUBJ.1294, SLE, week52


title: SUBJ.1222, SLE, week16


title: SUBJ.1294, SLE, baseline


title: SUBJ.0292, SLE, baseline


title: SUBJ.0720, SLE, week52


title: SUBJ.0133, SLE, baseline


title: SUBJ.0133, SLE, week16


title: SUBJ.0133, SLE, week52


title: SUBJ.0821, SLE, week52


title: SUBJ.0720, SLE, week16


title: SUBJ.0428, SLE, baseline


title: SUBJ.0821, SLE, baseline


title: SUBJ.0720, SLE, baseline


title: SUBJ.0428, SLE, week16


title: SUBJ.0792, SLE, baseline


title: SUBJ.0792, SLE, week16


title: SUBJ.0792, SLE, week52


title: SUBJ.0428, SLE, week52


title: SUBJ.1340, SLE, week52


title: SUBJ.1340, SLE, week16


title: SUBJ.1084, SLE, week16


title: SUBJ.0885, SLE, week52

value: multiple


title: SUBJ.1340, SLE, baseline


title: SUBJ.1421, SLE, week16


title: SUBJ.0885, SLE, baseline


title: SUBJ.1421, SLE, baseline


title: SUBJ.0885, SLE, week16


title: SUBJ.1203, SLE, week16


title: SUBJ.1421, SLE, week52


title: SUBJ.1203, SLE, baseline


title: SUBJ.0821, SLE, week16


title: SUBJ.1111, SLE, week52


title: SUBJ.1111, SLE, week16


title: SUBJ.0061, SLE, week16

value: asian


title: SUBJ.0061, SLE, week52


title: SUBJ.1612, SLE, week52


title: SUBJ.1612, SLE, week16


title: SUBJ.0061, SLE, baseline


title: SUBJ.1612, SLE, baseline